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Bill Text — Dec 27, 2012
H.R. 4203 (112th): Women's Procurement Program Improvement Act of 2012
This bill's text for status Reported by House Committee (Dec 27, 2012) is now available.
GovTrack Insider, our Kickstarter-backed work to summarize bills in Congress, has resumed. Here's what we've been up to:
Mar 19, 2012
112th Congress, 2011–2013
This bill was introduced on March 21, 2012, in a previous session of Congress, but was not enacted.
Representative for New York's 12th congressional district
Read Text »
Last Updated: Dec 27, 2012
Length: 8 pages
To amend the Small Business Act with respect to the procurement program for women-owned small business concerns, and for other purposes.
The bill's title was written by its sponsor.
This is the first step in the legislative process.
Reported by Committee
A committee has issued a report to the full chamber recommending that the bill be considered further. Only about 1 in 4 bills are reported out of committee.
This is a House of Representatives bill in the United States Congress (indicated by the "H.R." in "H.R. 4203"). A bill must be passed by both the House and Senate in identical form and then be signed by the President to become law.
The committee chair determines whether a bill will move past the committee stage.
There have been no roll call votes related to this bill.
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Civic Impulse. (2016). H.R. 4203 — 112th Congress: Women's Procurement Program Improvement Act of 2012. Retrieved from https://www.govtrack.us/congress/bills/112/hr4203
"H.R. 4203 — 112th Congress: Women's Procurement Program Improvement Act of 2012." www.GovTrack.us. 2012. February 14, 2016 <https://www.govtrack.us/congress/bills/112/hr4203>
{{cite web
|title=H.R. 4203 (112th)
|accessdate=February 14, 2016
|author=112th Congress (2012)
|date=March 19, 2012
|quote=Women's Procurement Program Improvement Act of 2012


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the article reports that
-Redbull type coanda. The reason why the 2012 coanda overheated the tyres is known. It had to do with the shape of the sidepod.
-Small gearbox with rear suspension designed so it can be changed quickly to suit the tyres.
-all crash tests passed
Thanks for this Timstr11
google translation
Mechanically the 2013er Silver Arrow has been a face. The new ultra-small gear has become ready in time and brought its first successful test runs behind. The crash tests were passed on time. The chassis has its baptism of fire in the lab already behind. The rear wheel suspension is designed so that it can change the geometry quickly when needed. The tire development requires that flexibility.
While Aldo Costa has the field of mechanical grip, about the aerodynamics is a question mark. The new Mercedes is very different from the old. One need not be a soothsayer that he is similar in many details to the Red Bull, especially around the exhaust. Course 2013 Mercedes continues with the Coanda solution. "This is the way to go," confirmed team boss Ross Brawn at the season finale in Sao Paulo. Meanwhile, the engineers know why the exhaust gases, the rear tires have heated up excessively. The side boxes were to blame. The exhaust jet was lost in free space, after he left the tailpipes and the Coanda slot. Red Bull he will continue beyond the side boxes and the bottom plate to the diffuser edge. Disturbing influences by the natural flow Adrian Newey met with two shafts, where he sucks the trending along the sidepods air into the vehicle. The same is expected from Mercedes.
Even Michael Schumacher is optimistic that many of the structural changes in the team last fall. "In the first two years, we definitely do not have the capacity to fight on several fronts. If there were problems, we had to take care of the problems. Since the development was interrupted. Only now there is an infrastructure that enables both. Properly will grab this infrastructure until 2013 and beyond. the car that we had last year, was indeed built under the old conditions. I'm talking about quantity, not quality. This has always been the reason why development stagnated. Why the last was still the case, I do not understand. Since we created the basic conditions for to come forward. But maybe have a few more cogs ineindergreifen in our structure, until that happens. "
Edited by BernieEc, 27 December 2012 - 11:50.

subset: commoncrawl


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Boon Tiger Hoodie$56.95SizeChestSmall34" - 36"Medium38" - 40"Large42" - 44"XLarge46" - 48"
Ahh yes, the beloved sweat shirt. Here at MMA Industries, we love our hoodies. No other garment offers the combination of warmth, comfort, and style that a quality, well-worn hoody does, just ask Adam Sandler. Just because it's getting a little chilly outside doesn't mean you can't show the world that you've chosen the path of the warrior. Take a look at our selection of quality sweatshirts and hoodies from top Muay Thai and MMA manufacturers. Besides being a comfortable way to beat the elements and show your style, the sweatshirt can also be a valuable training tool. Wearing one while you're warming up allows you to break that first sweat quicker, and it keeps your muscles warmer, reducing the chance of injury. Wear a hoody on that long walk to the cage or the ring and keep your warm up sweat going. The sweatshirt can also be a weight-cutting tool. We've all seen Vision Quest; sometimes you just gotta wear a sauna suit under a hoody to cut weight. Shop for your sweatshirts at MMA Industries and proudly proclaim your dedication to the fight sports to the world!
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The Art of The Craft is a series from The Ritz-Carlton that gives you a behind-the-scenes look at how our Ladies and Gentlemen create long-lasting memories for our guests. Each video focuses on a different individual from The Ritz-Carlton, exploring who they are and how they use a blend of talent, discipline and artistry within their craft.
The Ski Valet at The Ritz-Carlton, Bachelor Gulch creates personalized and memorable ski adventures for our guests they'll always remember. From expert ski tuning, to trail planning, he makes sure that all their ski equipment is as ready for the mountain as they are.
Like a symphony conductor, the General Manager of The Ritz-Carlton Shanghai, Pudong excels at bringing individual talents together to orchestrate a memorable experience for each and every guest at his hotel. His commitment to showing genuine care for his team, his guests and his hotel is immeasurable.

The cultural host at Al Bustan Palace, a Ritz-Carlton Hotel proudly shares his heritage with hotel guests. By making century-old traditions a brand new experience, he creates memories of Oman to last a lifetime.

The concierge at The Ritz-Carlton Residences, San Francisco has her finger on the pulse of the city she loves to provide her owners with unique and memorable experiences. Her inspiration comes from her own passion for making an ordinary request an extraordinary one, no matter what it takes.

The Naturalist at The Ritz-Carlton, Grand Cayman is a true champion of the environment. Inspired by her guests and love for the natural world, she crafts unique opportunities for children and adults alike to connect with their surroundings and introduces moments that last a lifetime.

Poised on the edge of famed Red Square, not far from the Kremlin,
 The Ritz-Carlton, Moscow offers premier accommodations with history surrounding you at every turn. There, the Pastry Chef consistently wows guests and visitors alike with his extraordinary work. On the recent occasion of the re-opening of the Bolshoi Theater after nearly six years of restoration, he designed a three-meter high gingerbread replica, with every architectural detail rendered to perfection. Displayed in the lobby of The Ritz-Carlton, hundreds of opera and ballet enthusiasts came to see this achievement and to photograph it so they would remember this artist's salute to the Bolshoi for years to come. Which is exactly why this pastry chef followed his childhood passion—to use his talents and artistry to find new and unexpected ways to please guests and create memories that will last far beyond their stay.

As a retreat, The Ritz-Carlton, Sanya is the perfect haven for families to escape the urban chaos with its large tropical garden hidden away inside a secluded mangrove forest. There, a true craftsman, in his own time, has created a secret garden for Ritz-Carlton guests to explore and enjoy. Uncle Martin's Secret Garden is the vision of the hotel's Director of Engineering who developed an expansive garden as part of the hotel's environmental protection program. All in his spare time with nearly every feature of this space is made from recycled materials. His unique vision has given parents an opportunity to revisit their own childhood in the eco-friendly garden with their children. He has made it not just a garden, but a setting for parents and children to connect, bond and share time that will be remembered and reflected upon for many years to come. In that light, it might be the perfect escape. A shining example of a craftsman at The Ritz-Carlton, he uses his passion, talents and artistry to find new and unexpected ways to please guests and create memories that will last far beyond their stay.

The florist at Hotel Arts Barcelona is a true artist, inspired by her guests and her love of simplicity. She loves that her job lets her share her passion for art and flowers, stimulating each guest's senses and creating indelible memories.

At The Ritz-Carlton Washington, D.C., the steward plays a pivotal role in all events. He focuses on every detail. Through constant communication he sees to it that no matter how large, last-minute or complex a function may be, to guests it is perfectly planned, as if it were choreographed and rehearsed for months.

The shoe shine valet at The Ritz-Carlton, Istanbul, and his approach to service are not soon forgotten. His meticulous care for shoes is legendary, but it's his willingness to measure his day by how many people he makes happy that leaves a lasting impression.

Walking into a room for the first time, the housekeeper at The Ritz-Carlton, Hong Kong looks for ways to comfort. Her gift is knowing the guest, understanding their preferences and seeing to it that every detail is attended to. Creating memories that can last a lifetime is simply part of her day.

The sommelier at The Ritz-Carlton, Half Moon Bay is happiest when introducing guests to new and interesting wines. He pays particular attention to flavor pairings and their ability to create the perfect meal and the memories of a lifetime.

At The Ritz-Carlton, Wolfsburg, the Chef de Cuisine plays a fundamental role in the experiences of our guests. Paying particular attention to personal service, his unique cooking techniques and craftsmanship create memorable dining experiences.

At the Ritz-Carlton, Tokyo, the sous chef creates unforgettable, award-winning desserts that typify the craftsmanship on display in all The Ritz-Carlton hotels. The chef was part of the Japanese national team whose seven-foot-tall chocolate piedmont finished second in 2011's la Coupe du Monde de la Patisserie contest. "I love to match desserts with main courses," he says. "It's a creative challenge because the dessert needs to be visually appealing as well as being a fresh and complementary taste to the main course." His signature dish, yuzu soufflé, marries the devilishly difficult French delicacy with a delicious Japanese seasonal fruit. Recently, he recreated the Tokyo Midtown Tower--in chocolate. His greatest thrill, however, is seeing the delighted faces of the guests as they encounter his creations.
The event designer at The Ritz-Carlton, Naples is inspired to craft meetings and social celebrations that wow every guest by fine tuning the smallest details, rising to the greatest challenges and making perfection his personal mantra. His motivation is the memories that become as treasured as the Gulf Coast sunsets.
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Costa Rica Art Vacations
Costa Rica has actually quietly been expanding in the art world, however in recent times there has actually been more of an establishing art neighborhood. While this island is located in an area that is heavily influenced by Pre-Columbia art, artists that are native and those that have elected to move to Costa Rica are producing brand-new and interesting works of art. For them it is a world of art where they are breaking new ground.
There are more than enough galleries in the capital of Costa Rica, San Jose. But there are no real "art" museums yet. One method of showing exactly what their artist's have is in the artists themselves showing there art in their own environments, their homes. There is a tour that takes you to 5 or 6 artist's homes in a day's tour, and you get a chance to truly experience the their work.… more
Asian Botanical Paintings Are One Of My Passions
I have a love for Asian botanical paintings. I have actually been seeking them out for a very long time. I have many in my collection and love every one of them.
The first Asian botanical painting that I bought was Vietnamese. It was one of a series of twelve paintings that I bought that were developed by Vu Viet Hung. I have them all over my home.
These oil landscapes by Vu Viet Hung are spectacular. These Asian botanical paintings truly set a serene tone in my home. The subtleties of the colors and the simple styles go so well on my walls.
I discovered a charming Asian botanical painting rather by chance at a garage sale. I don't normally make a routine of looking for paintings at garage sale, but this one was displayed out front and attracted me to stop. At first glimpse, I thought I was looking at a painting of a palm tree.… more
Preserving And Securing Your Valuable Oil Paintings
You could have simply bought your first oil painting, to include that touch of style to your study, living room, or bed room. Oil paintings are beautiful additions to the house, and are terrific conversation pieces also. They can likewise be a great investment, but for any of these draws of the oil painting to remain true they should be taken care of in a proper way. Right here are some basic steps in the preservation of the value and look of your new oil painting.
Constantly check the area where you plan to put your piece. Constantly prevent any area that is prone to severe heat or cold, or humidity. This consists of places like over the fireplace and in a front room that isn't really generally heated. Also keep in mind that prolonged sunshine will cause the paint to fade, and choose the location appropriately.
A layer of dust will also spoil the painting, as it will often dry out the oil colors out and therefore fade the piece.… more
Modern Oil Paintings
I have actually been purchasing modern-day oil paintings for a show in my gallery. I have discovered lots of nice pieces. I found a painting called Town in winter in a private collection in Illinois. The artist was Fern Isabel Coppedge and she was an American. I liked the piece since of the snowy scene. It advised me of my childhood.
While on a purchasing journey, I found Clouds Over Buckingham. This was a truly nice contemporary oil painting that was in a private collection in Pennsylvania. I have actually seen work by this artist prior to and it always resells very well.
Purchasing modern oil paintings has taken me to various websites throughout the United States. I specifically liked Main Line, Pennsylvania. That is where I obtained Leaning Silo. The artist of Leaning Silo was Arthur Meltzer. I had actually been formerly unpracticed him.
I enjoyed my trip to Long Island City.… more


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Fitness and Yoga Apparel
women's yoga and fitness clothing – workout in comfort and style!
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Customer Reviews for On Sale Fabric Family Room / Den Roman Window Shades
"Sophisticated Styling"
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The Roman shade is a perfect way to finish your window with a professional looking product. The light filtering fabric is great for blocking out the damaging suns rays, but letting the light still fill your room.View this product
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I purchased the roman shade for my bathroom window which is small but I wanted something nice. I added on the open down for privacy in the bathroom. It was a perfect choice!!!View this product


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Used Golf Drivers from Top Brands
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You are hereHome ›
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Earth and space science
Earth, moon and sun
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In this self-paced tutorial, learners examine data to determine the relative contribution of what we call natural climate cycles and that of human-caused greenhouse gases to contemporary climate change. Changes in the Earth-Sun geometry over time... (View More) are explored using interactive digital applets. A section supporting pedagogical techniques that begin with student prior conceptions and a list of common Earth science student misconceptions are included. Vocabulary terms are hot linked to a glossary. This is the sixth of ten self-paced professional development modules providing opportunities for teachers to learn about climate change through first-hand data exploration. (View Less)
Keywords: Greenhouse gases; Ice ages; Insolation; Louis Agassiz; Milankovich; Vostok ice core; Precession; Perihelion; Misconceptions
Audience: Vocational/professional development
Materials Cost: Free per student
Materials Cost: Free per student


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The Baker County Commission agreed to spend money that wasn't budgeted for recreational improvements after publicly acknowledging that some of the old bleachers at the Knabb Sports Complex fields in north Macclenny had deteriorated to the point of being dangerous.
Commissioners approved County Manager C.J. Thompson's recommendation to spend $18,194 to repair or replace most of the wooden bleachers after expressing concerns for public safety at the ball fields.
Commissioner James Croft, who was elected in November, cast the only dissenting vote, saying this was the first time he had heard of any safety issues with the bleachers. He said the time to discuss expenditures for repairs was before the 2012-13 budget went into effect on October 1.
"I doubt very seriously that those bleachers got that way since October 1," Mr. Croft said, adding that mid-year is not the time to increase expenditures in a deficit budget that was only balanced last year after an infusion of about $1.6 million in reserve funds.
"I'm not anti-Little League and not anti-safety, and I'm not anti-youth," he said. "But this board has got to face reality. We are on a down-hill pull."


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Energy, biomedical and health R&D included
About the Author
Alice Lipowicz is a staff writer covering government 2.0, homeland security and other IT policies for Federal Computer Week.
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Check out the digital edition of FCW magazine -- the federal IT community's premier publication.
In this issue: Tony Scott's busy year ahead, Rising Star profiles and more.
See the latest issue
DHS pulled a two-year-old solicitation for administrative support for cyber mission offices.
The president put the federal government's portfolio of aging IT systems front and center in his final budget request to Congress.
The Obama administration's proposed Cybersecurity National Action Plan for FY2017 includes $3.1B for IT modernization and a new federal chief information security officer.
Looking for success stories and exceptional change in federal IT? Meet the women and men who are making it happen.
In a new survey, federal leaders say they're making digital progress, but most also say they're merely updating old systems, not reinventing processes for the Digital Age.
The U.S. Customs and Immigration Services created new deputy CIO slot to help balance everyday IT operations and burgeoning DevOps, agile and cloud activities.
NIST is looking to increase trust in the technical underpinnings of encryption, by strengthening cryptographic random bit generators.
Steve Kelman applauds ASI Government for sharing valuable knowledge -- and notes that there are still plenty of other questions to answer.
In an op-ed for Business Insider, the GOP presidential candidate called for "the federal government must put its own house in order, prioritizing to reflect the urgency and importance of protecting key databases and communications."
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Symptoms usually occur 10-21 days after contact with the chickenpox virus. Initial symptoms include:
- Headache
- Fever
- General feeling of discomfort
- Loss of appetite
- Cough
Within 1-2 days after the initial symptoms, a rash develops. Characteristics of the rash include:
- At first, the rash consists of small, flat, red spots.
- The spots become raised and form clusters of round, itchy, fluid-filled blisters on a red base.
- New clusters form over 5-6 days.
- After the rash develops, there is usually a variety of spots visible: flat red areas, blisters with clear fluid, blisters with cloudy fluid, and open blisters.
- The rash usually develops on the skin above the waist, including the scalp. Exposed areas are often most affected.
- The rash may sometimes appear on the inside of the eyelids, in the mouth, nose, throat, upper airway, voice box, rectum, or vagina.
- In healthy children, the rash usually crusts over by day 6-7. The crusts are gone within three weeks, usually without scarring. Note: Adults or patients who are immunocompromised may have more severe cases that last longer.
Revision Information
- Reviewer: David L. Horn, MD, FACP
- Review Date: 03/2015 -
- Update Date: 03/15/2015 -


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Medicare Forms
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UB04 Condition Codes
UB04 Revenue Codes
786.09 - Other respiratory abnormalities
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UN.GIFT Publications
OSCE Guide on Gender-Sensitive Labour Migration Policies Russian
Jan 11 2011
Download right click "save as"
This guide attempts to analyse some of the most pressing gender issues in contemporary labour migration. It seeks to highlight the rationale for including gender-sensitive measures and ways to implement them into the migration policies of countries of origin and destination, as well as in bi- and multilateral agreements. By presenting good practices and providing tools on how to shape migration processes, the Guide aims to assist and encourage OSCE participating States and Partners for Co-operation to enforce current legislation as well as develop and adopt policies and programmes for female migrant workers that are more gender sensitive. In this way, it should encourage migration policies to be formed in a more gender-just way. This guide is also available in English on the website of OSCE.
- Walk Free Global Slavery Index 2013
- EHTN Case Study: Beulah London
- Manpower Group Case Study
- La Strada: Findings and Results of the European Action for Compensation for Trafficked Persons
- The Protection Project: 100 Best Practices in Combating Trafficking in Persons
- CSO_Ateneo_ Trafficking in Women and Children in ZAMBASULTA.pdf
- CSO_Annual Report 2009 - 2010
- Manpower-Verite - Ethical Framework
- Investor Guide for Effective Supply Chain Accountability
- LEFO: Qualitätsstandards
- LEFO: Quality Standards
- Summary of Rozrada Publications


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Harbour Club
Main Number: 843.723.9680
Our Story
Lunch Service[ X ]
Date: Wed, Dec 19, 2012
Time: 11:30am - 2:00pm
Add to your Calendar: Download Now
Lunch Service
11:30am to 2:00pm
The Club is the perfect place for a power lunch,
reconnecting with old friends or making new ones.
Lunch is served Tuesday through Friday in the Chairman's Grill,
Palmetto Room & Founding Governor's Lounge.
Enjoy the daily buffet, soup & salad bar, or order off our A La Carte Menu!
Members receive special buffet pricing. Business Casual Attire.
Reservations requested for parties of five or more.


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STUDENTS from around the globe are making Ambleside their home after enrolling on the University of Cumbria's first ever international course.
Outdoor enthusiasts from countries including China, Australia and Germany are among the 17 students spending the Autumn term in the Lake District as part of their masters in Transcultural European Outdoor Studies.
The qualification is the first of its kind and is being run by the university, Germany's University of Marburg and Norway's School of Sport Sciences.
Course leader Dr. Christopher Loynes said: "Within this time frame, our ambition is to make the course a coveted choice for people interested in pursuing a career in the outdoor field. We also aim to develop tight links with non-European universities and make the course truly global. Eventually, we plan to make the course financially self-sustaining through the charging of student fees."
The course will be funded by the European Commission for the next five years and once students have left Ambleside in the New Year they will spend their next two terms at the other institutions.
While some of the students area already experienced in outdoor studies, for many this is a new area of knowledge and expertise.
Katerina Pata from Greece, who previously studied to be a pre-school teacher, said:"I couldn't find a relevant masters course in Greece and applied for courses elsewhere in Europe. I liked this course because I get a chance to go to three different countries and learn various approaches to the subject. My goal is to make the concept of outdoor learning more prominent in my country and create my own outdoor kindergarten."
However, others come to the course with a lot of experience like Wilson Wai Yin Cheung who is a former president of the mountaineering association of Hong Kong.
The students, who come from 15 countries, will be living in university accommodation in Ambleside and learning the theoretical knowledge and practical qualifications necessary to be successful outdoor industry professionals.
During their first semester they will undertake two week-long hiking expeditions, one in the Scottish Highlands and one in the Lake District and a week-long canoeing trip.
Rothay Manor Hotel has been in every issue. "Standards are as high as ever: immaculate, beautifully furnished bedrooms and lounges; excellent food; a warm welcome."
To celebrate we received their Gold award in recognition of this achievement, one of only four hotels in the UK.
Web Development by Click Innovate


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Master Calendar
Wright Museum of Art Fall Exhibit(s) opening reception
From site: News & Events
Date: Thursday, September 5th, 2013
Time: 4:00 pm
Duration: 2 hours
The Wright Museum of Art
Sponsored by: The Wright Museum of Art
Contact: firstname.lastname@example.com, Joy Beckman 363-2097
Featuring Three Exhibits:
The Sacred Lives of Objects.
North Gallery, August 26 to November 10
This exhibition is student curated from Natalie Gummer's Religious Studies class and includes objects from both the Logan Museum of Anthropology and the Wright Museum of art.
There and Back Again: Pilgrims and Souvenirs
2013 Exhibition Workshop.
Neese Gallery, August 26 to November 10
The exhibition workshop is a five day workshop where students create, curate, plan and install an art exhibit. This year's exhibition takes a look at pilgrimage across the globe and the people and objects that return.
The Trash Caste
South Gallery, August 26 to October 28
An exhibition of the photographs of Amiran White; an associated press reporter who, among other assignments, explored the Mumbai Municipal Dump. This work is being exhibited in conjunction with the FYI reading; Behind the Beautiful Forevers, by Katherine Boo.
This event is free and open to the campus and community.


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Do you wish you had an easy way to move your tripod or pier mounted telescope from garage to patio or driveway? Wheeley Bars (telescope wheels/dolly) were created for just that purpose. The sturdy base with locking wheels and leveling bolts allows you to easily move, lock, level and use the complete telescope assembly. And best of all, you don't have to disconnect and reconnect all the electrical and computer cabling.
Copyright © 2016


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Whether you're out and about or at home, baby's sleep and play time is covered with traveller™ - the only portacot that is lighter than the baby.
How lightweight? A super light and compact 7lbs for travel near or far, traveller keeps your child happy and comfy either asleep in the cot or at play in the playpen.
See your baby clearly with traveller's full mesh sides. These sides have the added benefit of creating a gentle cocooned affect for peaceful sleeping as they remain slightly raised off the ground when you baby is inside. Globally safety certified for your peace of mind & your baby's comfort.


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Recent Forum Posts
View More
Recent Comments
- 2 days ago I Want one... Always wanted to try Samsung Android phones, never had a chance...
- 3 years ago I Hope I Win
- Member for
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Gimme that camera
Look before you buy
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Solid for the money
Portions of this page are modifications based on work created and shared by the Android Open Source Project
and used according to terms described in the Creative Commons 2.5 Attribution License. AndroidCentral is an independent site
that is not affiliated with or endorsed by Google.


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We encourage you to consider joining the supporters who have made a legacy gift to the University of Hartford. Read more.
Learn which type of gift may be appropriate at your stage of life.
Under Age 60 | Ages 60–70 | Over Age 70
© Pentera, Inc. Planned giving content. All rights reserved.


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Used Fairway Woods by the Hundreds
We get dozens and dozens of new arrival used fairway woods every day. Poke around to find the fairway wood that fits your game. 3balls.com is your place for top brand used fairway woods at discount golf prices.
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Fly from San Jose Costa Rica to San Andres, Colombia . Typical airfare prices on the San Jose Costa Rica (SJO) to San Andres (ADZ) flight route vary greatly depending on the season you are looking to book in.
Please note, our system could not find pricing for this route in our database cache. Please use the search box above to find up to date pricing. If no flights can be found for this route, please try to search again using the closest major cities.


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Digital Divide Open Lab Hours
Date and Time:Wednesday, June 5, 2013 - 10:30am–12:30pm
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H.E. Amb. Zachary D. Muburi-Muita, was on 21 st January, 2010 elected President
of the United Nations High-Level Committee on South-South Cooperation for a period
of two years.
The UN High-Level Committee on South-South Cooperation is an important organ in
the governance structure of South-South Cooperation within the UN System. The Committee
serves as the governing body for South-South Cooperation in the UN General Assembly.
It collaborates with and is supported by the Special Unit for South-South Cooperation,
as well as the global South-South Cooperation focal points system at National level
and within the UN agencies. The High-Level Committee also oversees the intergovernmental
review and policy making of SouthSouth Cooperation. It meets biennially and its
decisions are endorsed by the UN General Assembly as the basis for strengthening
South-South Cooperation.
The next High-Level Committee meeting, scheduled to take place on February 4, 2010
at the UN Headquarters in New York, will be the first major decision-making session
presided over by the newly-elected President. The meeting is expected to agree on
the agenda and organization of work as well as key activities and programmes to
advance South-South Cooperation. Of critical importance will be a decision on how
to follow-up and fully implement the Nairobi Outcome on South-South Cooperation
following the historic UN High-Level Conference on South-South Cooperation that
Kenya was honoured to host in Nairobi from 1st – 3rd December, 2009.
The election Amb. Zachary D. Muburi-Muita as President of the High-Level Committee
is an indication of the confidence the UN Member States have on Kenya and an appreciation
of H.E. Ambassador Muburi-Muita's, as well as the Kenya Mission's contribution to
issues on the agenda of the UN, particularly those of interest to developing countries.
Other members of the Bureau of the High-Level Committee who were elected are Suriname
and Canada as Vice-Presidents, representing the Latin American and Caribbean States
and the Western European and other States Group respectively. The Philippines, representing
the Asian States Group, was elected the Rapporteur. The nominee for the Eastern
European Group is yet to be decided upon.
Ambassador Muburi-Muita is already serving as Vice-President of the Bureau of the
Assembly of States Parties to the Rome Statute of the International Criminal Court
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text:
2002 Mustang Parts
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If there's any issue that routinely frustrates executives in many organizations, it's how to get a true fix on the value that information technology adds to the businesses it serves. IT is undoubtedly central to creating value and therefore continues to account for a rising share of total investment. But defining, measuring, and maximizing that value remain elusive. To throw light on this crucial issue, McKinsey collaborated with CIGREF1 1.
CIGREF, the "Club Informatique des Grandes Entreprises Françaises," founded in 1970, strives to "promote the use of information systems as a driver of value creation and a source of innovation." It includes more than a hundred public and private organizations from every economic sector in France. to study the best practices of major French and international companies across various sectors.2 2.
"The dynamics of information-system-driven value creation," CIGREF and McKinsey & Company, 2008.
We interviewed 11 CIOs from French companies over a period from March 2007 to November 2007. The in-depth nature of these interviews provided valuable insights, as it allowed us to draw directly from the experiences of CIOs—many of whose companies have successfully used IT to gain competitive advantage. Analyzing their approaches to information technology helps to show how it can promote economic performance. We complemented these insights with international case examples.
IT generates value at two complementary levels (Exhibit 1). The core asset value includes tangible items such as hardware and software, as well as softer benefits such as the IT organization's processes and skills. IT's vitally important value-in-use varies with a company's core business priorities, such as whether it aims for an organizational transformation or operational excellence. A different set of metrics is needed to measure value-in-use, to account for both its economic and strategic dimension.
IT generates value at two complementary levels: the core asset value (eg, hardware and software) and the vitally important value-in-use.
Take the example of a group focused on optimizing investments among its various businesses—say, a banking group with multiple business units such as retail banking, consumer finance, capital markets, asset management, and the like. The economic value expected from the IT department can be measured through the improvement in the overall cost-to-revenue ratio, while the strategic value can translate into a competitive edge in terms of investment or acquisition capacity. (Since 80 to 90 percent of all synergies from banking mergers involve reducing the cost of operations, IT is indeed a key enabling factor during an acquisition.) The indicators that are tracked will be mainly financial, such as the ratio of IT spending to revenue, and will then be compared with the operating ratio—for example, operating costs over revenue (Exhibit 2).
In the context of optimizing investments, the value of IT derives from dynamic control over the impact of IT investments on each company's operating ratios.
Similarly, in companies for which the priority is operational excellence (understood as quality and productivity of processes), the business value from IT will be measured in terms of key performance indicators (KPIs) at the process level. For example, IT will be seen as valuable if the systems helped to reduce the delay for processing an insurance claim or to ensure a no-error delivery of supplies to the production line (Exhibit 3).
At one global logistics company in our study, IT greatly improved supply chain operations—a key factor in a radical transformation—by helping the company to optimize its parcel-loading and truck-routing activities and to develop new value-added services, such as same-day delivery and made-to-order solutions for customers. IT also provided important data for more efficient risk management and better pricing.
For CIOs focused on operational improvements, IT's value is measured mainly by process performance indicators: productivity, on-time delivery, and quality.
Traditionally, a CIO's main responsibility has been using standard practices and performance measures to maintain IT's asset value. Developing value-in-use is a different ball game, however, and CIOs need to examine new levers found at points where the IT department and the business units intersect (Exhibit 4). To succeed, CIOs must take on new roles—bridging functional silos—that may take them beyond their comfort zones. For one thing, they will need to collaborate with executives in the business units to work on major transformation projects, to coordinate strategic planning, or to manage investments collaboratively (see sidebar following this article, "Next steps: Identifying the challenges").
Developing value-in-use requires a CIO to examine new levers found at points where the IT department and the business units intersect.
Cementing new alliances within the organization is critical (Exhibit 5). A CIO in charge of optimizing IT investments at the group level will need to assume responsibility for managing a portfolio of investments. To do so effectively, the CIO will have to join forces with the CFO, who has expertise in maximizing returns on investment. If the corporate goal is operational excellence, HR is more likely to be the CIO's preferred ally. This is due to the critical role of change management. Take the example of deploying a new enterprise-resource-planning (ERP) system: the critical challenge is ensuring that the target processes are codified correctly in the system, and that when it is implemented, the end users are sufficiently trained to effectively leverage the potential of the new tool. This requires a joint effort from HR and IT to synchronize and coordinate their tasks from the initial design to the rollout and subsequent life of the system.
The CIO builds alliances with peers in areas relevant to supporting the company's priorities.
The businesses that are the best at creating value-in-use, we found, embed their IT governance within the broader governance practices. In practical terms, this requires IT representatives to participate in company forums that traditionally have been the exclusive domain of business unit leaders. At successful companies, certain core business processes, such as managing the business project portfolio or determining the allocation of resources, dovetail with IT processes. This notion of an integrated business–IT governance model can also apply the other way around: we have witnessed examples of companies where strategic planning for IT actually serves as a platform for broader strategic planning by establishing mixed business–IT forums (Exhibit 6).
The best companies at creating value-in-use embed their IT governance within the broader governance practices.
Next steps: Identifying the challenges
At the best companies in our study, the CIO, the CEO, and the business units essentially cocreate value-in-use when they integrate the elements of our framework (Exhibit A). But to tap the potential reservoir of value, the new partners must have a clear view of the challenges they face (Exhibit B).
CIOs need to understand what the business units expect from the IT organization and to articulate IT goals in terms that business leaders can grasp. That means eschewing technology jargon, making innovative proposals, and taking firm positions on cross-functional projects. To be secure in these new roles, CIOs must develop a range of skills that transcend their IT core competencies; to give the emerging collaborative effort better grounding, they should create forums where IT and the business units can set common priorities.
For CEOs and business unit leaders, the main issue is mind-sets: they need to stop thinking of IT as a service provider and consider ways to build alliances with IT executives. IT priorities should be set in clear business terms. Leaders of businesses should proactively draw their IT counterparts into strategic and operational planning sessions.
When this approach works, it produces a range of benefits. Fresh synergies between IT and the business units create a wider palette of skills for both as they take ownership of shared projects and increase the intensity of their interactions. With leaders from the two groups finally reading from the same script, communications become more efficient, since less translation time elapses between the formulation of business plans and their execution by IT. Of course, the real bottom line is that these benefits combine to raise IT's value-in-use across the enterprise.
A simple framework summarizes the best practices we observed in our interviews (see Exhibit A in sidebar, "Next steps: Identifying the challenges"). The value-in-use of information technology emerges when the IT department, building on a foundation of core performance, attacks problems and seeks solutions in areas that interest the business units and IT alike. Alliances with business leaders create new roles for CIOs and increase their scope of action. Governance practices that bring IT and business leaders together institutionalize this new way of operating.
Michael Bloch and Andres Hoyos-Gomez are principals in McKinsey's Paris office.
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text:
Lines Written in Early Spring
Source: The Longman Anthology of Poetry (Pearson, 2006)
Discover this poem's context and related poetry, articles, and media.
Poet William Wordsworth 1770–1850
POET'S REGION England
SCHOOL / PERIOD Romantic
Subjects Nature, Spring, Social Commentaries
Poems by William Wordsworth
- "Why art thou silent! Is thy love a plant"
- A Complaint
- A Poet! He Hath Put his Heart to School
- A Slumber did my Spirit Seal
- Character of the Happy Warrior
More poems by William Wordsworth (58 poems)
- Composed upon Westminster Bridge, September 3, 1802
- Elegiac Stanzas Suggested by a Picture of Peele Castle in a Storm, Painted by Sir George Beaumont
- Extempore Effusion upon the Death of James Hogg
- I Travelled among Unknown Men
- I Wandered Lonely as a Cloud
- Influence of Natural Objects in Calling Forth and Strengthening the Imagination in Boyhood and Early Youth
- Inside of King's College Chapel, Cambridge
- It is a Beauteous Evening, Calm and Free
- It is not to be Thought of
- Laodamia
- Lines Composed a Few Miles above Tintern Abbey, On Revisiting the Banks of the Wye during a Tour. July 13, 1798
- London, 1802
- Most Sweet it is
- Mutability
- November, 1806
- Nuns Fret Not at Their Convent's Narrow Room
- Nutting
- October, 1803
- Ode to Duty
- Ode: Intimations of Immortality from Recollections of Early Childhood
- On the Departure of Sir Walter Scott from Abbotsford, for Naples
- On the Extinction of the Venetian Republic
- Resolution and Independence
- Scorn not the Sonnet
- September, 1819
- She Dwelt among the Untrodden Ways
- She Was a Phantom of Delight
- Simon Lee: The Old Huntsman
- Song at the Feast of Brougham Castle upon the Restoration of Lord Clifford, the Shepherd, to the Estates and Honours of his Ancestors
- Sonnets from The River Duddon: After-Thought
- Surprised by Joy
- The French Revolution as It Appeared to Enthusiasts at Its Commencement
- The Green Linnet
- The Power of Armies is a Visible Thing
- from The Prelude: Book 1: Childhood and School-time
- from The Prelude: Book 2: School-time (Continued)
- The Reverie of Poor Susan
- The Simplon Pass
- The Solitary Reaper
- The Tables Turned
- The Thorn
- The Virgin
- The World Is Too Much With Us
- There was a Boy
- Three Years She Grew
- To a Highland Girl
- To a Skylark
- To the Cuckoo
- We Are Seven
- Written in London. September, 1802
- Yarrow Revisited
- Yarrow Unvisited
- Yarrow Visited. September, 1814
Poem Categorization
SUBJECT Nature, Spring, Social Commentaries
POET'S REGION England
SCHOOL / PERIOD Romantic
If you disagree with this poem's categorization, make a suggestion.


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James William "Bill" Davis, 84, of Jacksonville died February 24, 2012. He was a member of Evangel Temple Assembly of God and he will be greatly missed. Bill was preceded in death by daughter Stacey Ann.
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Executives are increasingly positive about current economic conditions at both the global and national levels, according to our most recent survey on economic conditions.1 1.
The online survey was in the field from March 5 to March 9, 2012, and received responses from 2,060 executives representing the full range of regions, industries, company sizes, titles, and functional specialties. The data are weighted by the contribution of each respondent's nation to global GDP to adjust for differences in response rates.
The future of the eurozone economy remains uncertain—60 percent expect a minimal contraction or recession there within the next six months—but, compared with three months ago, significantly larger shares of executives around the world expect improving conditions in their own countries. Concern about sovereign-debt default as a barrier to growth is down, while worries about commodity prices and geopolitical instability are up since December.
There are also positive indicators at the company level: low demand is still the most-cited risk to growth in executives' countries, yet larger shares expect demand for their companies' products and services to increase. Profit expectations continue their upward trend, while workforce changes and investment decisions are consistent with results from three months ago, perhaps indicating broadly that companies aren't yet ready to move on these newly optimistic expectations.
For the first time since June 2011, executives are more optimistic than pessimistic about their national economies (Exhibit 1). The share saying economic conditions in their countries are better now than six months ago has more than doubled since December, even in the eurozone, and an even larger share expect their economies will be better in six months.
Unlike what we saw in December, expectations appear to be moving in a more consistent, upward direction across regions (Exhibit 2). Sixty-five percent of executives in India—the largest share—expect their economy to improve, while 59 percent in North America and 49 percent in developed Asia say the same.
At the regional level, expectations about unemployment vary. Respondents in North America notably have the most positive view, with 52 percent expecting the rate to decrease in the next six months. In developing markets,2 2.
Includes China and Latin America.
a nearly equal share expect the unemployment rate in their countries to stay the same, and only 22 percent predict a decrease.
At the global level, concerns about inflation rates have held steady but differ across regions (Exhibit 3). Inflation is cited as a risk to economic growth in respondents' countries by the same share as three months ago, yet views on other risks have shifted notably since December (Exhibit 4). Low consumer demand still tops the list but is now followed by government regulation, geopolitical instability, and lack of credit; three months ago, volatility and sovereign-debt defaults were in the top three.
Executives' optimism also extends to the global economy. Indeed, 42 percent say conditions are better now compared with six months ago, and 48 percent expect better conditions six months from now—up from 26 percent in December. Respondents in India report the most positive outlook on the world's prospects, and the share in the eurozone expecting improvement climbed 17 percentage points since the previous survey.
The eurozone remains, unsurprisingly, a locus of uncertainty: 60 percent expect either a minimal contraction or recession there in six months, while only 23 percent expect at least minimal growth. When asked about potential eurozone outcomes, fewer expect either short-term or long-term integration than in December, though nearly half expect rescue packages to maintain the status quo. A larger share in Europe expect integration than do their peers in other regions.
Executives expect uncertainty in other areas, which hints that the recent optimism may still be tenuous. One area of concern is around resources: 58 percent say oil prices will be higher in six months, and a growing share (19 percent, up from 11 percent in December) cite high commodity prices as a barrier to growth. More executives also cite geopolitical instability as a barrier to growth than did three months ago; at 31 percent, it's the barrier cited most often in North America.
While low demand is still seen as the biggest threat to growth, more respondents expect customer demand to increase than have said so since last June (Exhibit 5), and executives are especially positive in emerging economies. The share expecting their companies' profits to increase continues to grow: 62 percent say so now, up from 56 percent in December and 45 percent in September. As in December, executives in the eurozone are less positive about profits than elsewhere, but even their overall expectations have improved over time.3 3.
At 74 percent, respondents in India are most likely to say their companies' profits will increase in the next six months, followed by respondents in North America (69 percent), developing markets (66 percent), developed Asia (59 percent), non-eurozone Europe (54 percent), and eurozone countries (52 percent).
Executives report smaller changes with respect to their companies' hiring and investment decisions: they are still likeliest to say their workforce size will stay the same in the next six months,4 4.
Forty-eight percent say they expect the size of their companies' workforce to stay the same, 32 percent expect an increase, and 18 percent expect a decrease.
and slightly smaller shares say their companies are postponing capital investment decisions. They indicate no change since December in their companies' willingness to pursue (or not pursue) M&A deals.5 5.
Thirty percent say their companies are postponing or deciding not to pursue capital investments they would typically consider good for growth, compared with 33 percent in December. In both this survey and December's, 24 percent say their companies are postponing M&A decisions they would consider good for growth.
These results also seem to reflect a cautious optimism: while executives are more positive than not on the future of the economy as a whole, their companies aren't readier to make major strategic decisions than they were in December—or perhaps they lack confidence that conditions will improve substantively.
December 2015—This continually updated interactive tracks how executives around the world have viewed economic conditions and the economic prospects of their companies, and how those views have differed over time and across industries, regions, and types of company.more
December 2011—Executives' views about the global economy and their companies' prospects in most regions are more positive than in September, though they are still gloomier than in June.more
March 2012—The Pulitzer Prize–winning author and global energy expert sees rising demand from the East spurring innovation.more
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This page describes Bio.SeqIO, the standard Sequence Input/Output interface for BioPython 1.43 and later. For implementation details, see the SeqIO development page.
There is a whole chapter in the Tutorial (PDF) on Bio.SeqIO, and although there is some overlap it is well worth reading in addition to this WIKI page. There is also the API documentation (which you can read online, or from within Python with the help command).
Bio.SeqIO provides a simple uniform interface to input and output assorted sequence file formats (including multiple sequence alignments), but will only deal with sequences as SeqRecord objects. There is a sister interface Bio.AlignIO for working directly with sequence alignment files as Alignment objects.
The design was partly inspired by the simplicity of BioPerl's SeqIO. In the long term we hope to match BioPerl's impressive list of supported sequence file formats and multiple alignment formats.
Note that the inclusion of Bio.SeqIO (and Bio.AlignIO) in Biopython does lead to some duplication or choice in how to deal with some file formats. For example, Bio.Nexus will also read sequences from Nexus files - but Bio.Nexus can also do much more, for example reading any phylogenetic trees in a Nexus file.
My vision is that for manipulating sequence data you should try Bio.SeqIO as your first choice. Unless you have some very specific requirements, I hope this should suffice.
File Formats
This table lists the file formats that Bio.SeqIO can read, write and index, with the Biopython version where this was first supported (or git to indicate this is supported in our latest in development code). The format name is a simple lowercase string. Where possible we use the same name as BioPerl's SeqIO and EMBOSS.
|Format name
|Reads the ABI "Sanger" capillary sequence traces files, including the PHRED quality scores for the base calls. This allows ABI to FASTQ conversion. Note each ABI file contains one and only one sequence (so there is no point in indexing the file).
|Reads the contig sequences from an ACE assembly file. Uses Bio.Sequencing.Ace internally
|The alignment format of Clustal X and Clustal W.
|The EMBL flat file format. Uses Bio.GenBank internally.
|This refers to the input FASTA file format introduced for Bill Pearson's FASTA tool, where each record starts with a ">" line. Resulting sequences have a generic alphabet by default.
|fastq-sanger or fastq
|FASTQ files are a bit like FASTA files but also include sequencing qualities. In Biopython, "fastq" (or the alias "fastq-sanger") refers to Sanger style FASTQ files which encode PHRED qualities using an ASCII offset of 33. See also the incompatible "fastq-solexa" and "fastq-illumina" variants used in early Solexa/Illumina pipelines, Illumina pipeline 1.8 produces Sanger FASTQ.
|FASTQ files are a bit like FASTA files but also include sequencing qualities. In Biopython, "fastq-solexa" refers to the original Solexa/Illumina style FASTQ files which encode Solexa qualities using an ASCII offset of 64. See also what we call the "fastq-illumina" format.
|FASTQ files are a bit like FASTA files but also include sequencing qualities. In Biopython, "fastq-illumina" refers to early Solexa/Illumina style FASTQ files (from pipeline version 1.3 to 1.7) which encode PHRED qualities using an ASCII offset of 64. For good quality reads, PHRED and Solexa scores are approximately equal, so the "fastq-solexa" and "fastq-illumina" variants are almost equivalent.
|genbank or gb
|1.48 / 1.51
|The GenBank or GenPept flat file format. Uses Bio.GenBank internally for parsing. Biopython 1.48 to 1.50 wrote basic GenBank files with only minimal annotation, while 1.51 onwards will also write the features table (see Bug 2294).
|This refers to the IntelliGenetics file format, apparently the same as the MASE alignment format.
|This refers to the IMGT variant of the EMBL plain text file format.
|The NEXUS multiple alignment format, also known as PAUP format. Uses Bio.Nexus internally.
|PHD files are output from PHRED, used by PHRAP and CONSED for input. Uses Bio.Sequencing.Phd internally.
|An alignment format. Truncates names at 10 characters.
|A "FASTA like" format introduced by the National Biomedical Research Foundation (NBRF) for the Protein Information Resource (PIR) database, now part of UniProt.
|Simple sequence XML file format.
|Standard Flowgram Format (SFF) files produced by 454 sequencing.
|The Stockholm alignment format is also known as PFAM format.
|Swiss-Prot aka UniProt format. Uses Bio.SwissProt internally. See also the UniProt XML format.
|Simple two column tab separated sequence files, where each line holds a record's identifier and sequence. For example, this is used by Aligent's eArray software when saving microarray probes in a minimal tab delimited text file.
|Qual files are a bit like FASTA files but instead of the sequence, record space separated integer sequencing values as PHRED quality scores. A matched pair of FASTA and QUAL files are often used as an alternative to a single FASTQ file.
|UniProt XML format, successor to the plain text Swiss-Prot format.
With Bio.SeqIO you can treat sequence alignment file formats just like any other sequence file, but the new Bio.AlignIO module is designed to work with such alignment files directly. You can also convert a set of SeqRecord objects from any file format into an alignment - provided they are all the same length. Note that when using Bio.SeqIO to write sequences to an alignment file format, all the (gapped) sequences should be the same length.
Sequence Input
The main function is Bio.SeqIO.parse() which takes a file handle and format name, and returns a SeqRecord iterator. This lets you do things like:
from Bio import SeqIO handle = open("example.fasta", "rU") for record in SeqIO.parse(handle, "fasta") : print record.id handle.close()
In the above example, we opened the file using the built-in python function open. The argument 'rU' means open for reading using universal readline mode - this means you don't have to worry if the file uses Unix, Mac or DOS/Windows style newline characters.
Note that you must specify the file format explicitly, unlike BioPerl's SeqIO which can try to guess using the file name extension and/or the file contents. See Explicit is better than implicit (The Zen of Python).
If you had a different type of file, for example a Clustalw alignment file such as 'opuntia.aln' which contains seven sequences, the only difference is you specify "clustal" instead of "fasta":
from Bio import SeqIO handle = open("opuntia.aln", "rU") for record in SeqIO.parse(handle, "clustal") : print record.id handle.close()
Iterators are great for when you only need the records one by one, in the order found in the file. For some tasks you may need to have random access to the records in any order. In this situation, use the built in python list function to turn the iterator into a list:
from Bio import SeqIO handle = open("example.fasta", "rU") records = list(SeqIO.parse(handle, "fasta")) handle.close() print records[0].id #first record print records[-1].id #last record
Another common task is to index your records by some identifier. For small files we have a function Bio.SeqIO.to_dict() to turn a SeqRecord iterator (or list) into a dictionary (in memory):
from Bio import SeqIO handle = open("example.fasta", "rU") record_dict = SeqIO.to_dict(SeqIO.parse(handle, "fasta")) handle.close() print record_dict["gi:12345678"] #use any record ID
The function Bio.SeqIO.to_dict() will use the record ID as the dictionary key by default, but you can specify any mapping you like with its optional argument, key_function.
For larger files, it isn't possible to hold everything in memory, so Bio.SeqIO.to_dict() is not suitable. Biopython 1.52 inwards includes the Bio.SeqIO.index() function for this situation, but you might also consider BioSQL.
from Bio import SeqIO record_dict = SeqIO.index("example.fasta", "fasta") print record_dict["gi:12345678"] #use any record ID
Biopython 1.45 introduced another function, Bio.SeqIO.read(), which like Bio.SeqIO.parse() will expect a handle and format. It is for use when the handle contains one and only one record, which is returned as a single SeqRecord object. If there are no records, or more than one, then an exception is raised:
from Bio import SeqIO record = SeqIO.read(open("single.fasta"), "fasta")
For the related situation where you just want the first record (and are happy to ignore any subsequent records), you can use the iterator's .next() method:
from Bio import SeqIO first_record = SeqIO.parse(open("example.fasta", "rU"), "fasta").next()
Sequence Output
For writing records to a file use the function Bio.SeqIO.write(), which takes a SeqRecord iterator (or list), output handle and format string:
from Bio import SeqIO sequences = ... # add code here output_handle = open("example.fasta", "w") SeqIO.write(sequences, output_handle, "fasta") output_handle.close()
There are more examples in the following section on converting between file formats.
Note that if you are writing to an alignment file format, all your sequences must be the same length.
If you supply the sequences as a SeqRecord iterator, then for sequential file formats like Fasta or GenBank, the records can be written one by one. Because only one record is created at a time, very little memory is required. See the example below filtering a set of records.
On the other hand, for interlaced or non-sequential file formats like Clustal, the Bio.SeqIO.write() function will be forced to automatically convert an iterator into a list. This will destroy any potential memory saving from using an generator/iterator approach.
File Format Conversion
Suppose you have a GenBank file which you want to turn into a Fasta file. For example, lets consider the file 'cor6_6.gb' which is included in the Biopython unit tests under the GenBank directory.
You could read the file like this, using the Bio.SeqIO.parse() function:
from Bio import SeqIO input_handle = open("cor6_6.gb", "rU") for record in SeqIO.parse(input_handle, "genbank") : print record input_handle.close()
Notice that this file contains six records. Now instead of printing the records, let's pass the SeqRecord iterator to the Bio.SeqIO.write() function, to turn this GenBank file into a Fasta file:
from Bio import SeqIO input_handle = open("cor6_6.gb", "rU") output_handle = open("cor6_6.fasta", "w") sequences = SeqIO.parse(input_handle, "genbank") count = SeqIO.write(sequences, output_handle, "fasta") output_handle.close() input_handle.close() print "Converted %i records" % count
Or more concisely using the Bio.SeqIO.convert() function (in Biopython 1.52 or later), just:
from Bio import SeqIO count = SeqIO.convert("cor6_6.gb", "genbank", "cor6_6.fasta", "fasta") print "Converted %i records" % count
In this example the GenBank file started like this:
LOCUS ATCOR66M 513 bp mRNA PLN 02-MAR-1992 DEFINITION A.thaliana cor6.6 mRNA. ACCESSION X55053 VERSION X55053.1 GI:16229 ...
The resulting Fasta file looks like this:
>X55053.1 A.thaliana cor6.6 mRNA. AACAAAACACACATCAAAAACGATTTTACAAGAAAAAAATA... ...
Note that all the Fasta file can store is the identifier, description and sequence.
By changing the format strings, that code could be used to convert between any supported file formats.
Input/Output Example - Filtering by sequence length
While you may simply want to convert a file (as shown above), a more realistic example is to manipulate or filter the data in some way.
For example, let's save all the "short" sequences of less than 300 nucleotides to a Fasta file:
from Bio import SeqIO short_sequences = [] # Setup an empty list for record in SeqIO.parse(open("cor6_6.gb", "rU"), "genbank") : if len(record.seq) < 300 : # Add this record to our list short_sequences.append(record) print "Found %i short sequences" % len(short_sequences) output_handle = open("short_seqs.fasta", "w") SeqIO.write(short_sequences, output_handle, "fasta") output_handle.close()
If you know about list comprehensions then you could have written the above example like this instead:
from Bio import SeqIO input_seq_iterator = SeqIO.parse(open("cor6_6.gb", "rU"), "genbank") #Build a list of short sequences: short_sequences = [record for record in input_seq_iterator \ if len(record.seq) < 300] print "Found %i short sequences" % len(short_sequences) output_handle = open("short_seqs.fasta", "w") SeqIO.write(short_sequences, output_handle, "fasta") output_handle.close()
I'm not convinced this is actually any easier to understand, but it is shorter.
However, if you are using Python 2.4 or later, and you are dealing with very large files with thousands of records, you could benefit from using a generator expression instead. This avoids creating the entire list of desired records in memory:
from Bio import SeqIO input_seq_iterator = SeqIO.parse(open("cor6_6.gb", "rU"), "genbank") short_seq_iterator = (record for record in input_seq_iterator \ if len(record.seq) < 300) output_handle = open("short_seqs.fasta", "w") SeqIO.write(short_seq_iterator, output_handle, "fasta") output_handle.close()
Remember that for sequential file formats like Fasta or GenBank, Bio.SeqIO.write() will accept a SeqRecord iterator. The advantage of the code above is that only one record will be in memory at any one time.
However, as explained in the output section, for non-sequential file formats like Clustal write is forced to automatically turn the iterator into a list, so this advantage is lost.
If this is all confusing, don't panic and just ignore the fancy stuff. For moderately sized datasets having too many records in memory at once (e.g. in lists) is probably not going to be a problem.
Using the SEGUID checksum
In this example, we'll use Bio.SeqIO with the Bio.SeqUtils.CheckSum module (in Biopython 1.44 or later). First of all, we'll just print out the checksum for each sequence in the GenBank file ls_orchid.gbk:
from Bio import SeqIO from Bio.SeqUtils.CheckSum import seguid for record in SeqIO.parse(open("ls_orchid.gbk"), "genbank") : print record.id, seguid(record.seq)
You should get this output:
Z78533.1 JUEoWn6DPhgZ9nAyowsgtoD9TTo Z78532.1 MN/s0q9zDoCVEEc+k/IFwCNF2pY ... Z78439.1 H+JfaShya/4yyAj7IbMqgNkxdxQ
Now lets use the checksum function and Bio.SeqIO.to_dict() to build a SeqRecord dictionary using the SEGUID as the keys. The trick here is to use the python lambda syntax to create a temporary function to get the SEGUID for each SeqRecord - we can't use the seguid function directly as it only works on Seq objects or strings.
from Bio import SeqIO from Bio.SeqUtils.CheckSum import seguid seguid_dict = SeqIO.to_dict(SeqIO.parse(open("ls_orchid.gbk"), "genbank"), lambda rec : seguid(rec.seq)) record = seguid_dict["MN/s0q9zDoCVEEc+k/IFwCNF2pY"] print record.id print record.description
Giving this output:
Z78439.1 P.barbatum 5.8S rRNA gene and ITS1 and ITS2 DNA.
Random subsequences
This script will read a Genbank file with a whole mitochondrial genome (e.g. the tobacco mitochondrion, Nicotiana tabacum mitochondrion NC_006581), create 500 records containing random fragments of this genome, and save them as a fasta file. These subsequences are created using a random starting points and a fixed length of 200.
from Bio import SeqIO from Bio.SeqRecord import SeqRecord from random import randint #There should be one and only one record, the entire genome: mito_record = SeqIO.read(open("NC_006581.gbk"), "genbank") mito_frags=[] limit=len(mito_record.seq) for i in range(0, 500) : start=randint(0,limit-200) end=start+200 mito_frag=mito_record.seq[start:end] record=SeqRecord(mito_frag,'fragment_%i' % (i+1),'','') mito_frags.append(record) output_handle = open("mitofrags.fasta", "w") SeqIO.write(mito_frags, output_handle, "fasta") output_handle.close()
That should give something like this as the output file,
>fragment_1 TGGGCCTCATATTTATCCTATATACCATGTTCGTATGGTGGCGCGATGTTCTACGTGAAT CCACGTTCGAAGGACATCATACCAAAGTCGTACAATTAGGACCTCGATATGGTTTTATTC TGTTTATCGTATCGGAGGTTATGTTCTTTTTTGCTCTTTTTCGGGCTTCTTCTCATTCTT CTTTGGCACCTACGGTAGAG ... >fragment_500 ACCCAGTGCCGCTACCCACTTCTACTAAGGCTGAGCTTAATAGGAGCAAGAGACTTGGAG GCAACAACCAGAATGAAATATTATTTAATCGTGGAAATGCCATGTCAGGCGCACCTATCA GAATCGGAACAGACCAATTACCAGATCCACCTATCATCGCCGGCATAACCATAAAAAAGA TCATTAAAAAAGCGTGAGCC
Writing to a string
Sometimes you won't want to write your SeqRecord object(s) to a file, but to a string. For example, you might be preparing output for display as part of a webpage. If you want to write multiple records to a single string, use StringIO to create a string-based handle. The Tutorial (PDF) has an example of this in the SeqIO chapter.
For the special case where you want a single record as a string in a given file format, Biopython 1.48 added a new format method:
from Bio import SeqIO mito_record = SeqIO.read(open("NC_006581.gbk"), "genbank") print mito_record.format("fasta")
The format method will take any output format supported by Bio.SeqIO where the file format can be used for a single record (e.g. "fasta", "tab" or "genbank"). Note that we don't recommend you use this for file output - using Bio.SeqIO.write() is faster and more general.
If you are having problems with Bio.SeqIO, please join the discussion mailing list (see mailing lists).
If you think you've found a bug, please report it on RedMine.


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The module works great but the css for the registration page is messed up.
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I've noticed that since Pligg 2.0.0 has come out it's become increasingly more difficult for a module to appear the same across all templates. In your case I believe it's simply that the additional dialog it's adding to the instructions is pushing down some other stuff..
A simple and quick work around would be to open abyss_antispam_ini.php and comment out or delete line 12
Then clear your cache from the main admin panel.
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