text:
Istanbul on track for return to F1Comment on this story
Turkey's state-run agency says authorities have agreed "in principle" for Istanbul to return to the Formula One calendar in 2013.
Anadolu Agency quotes businessman Vural Ak - who has taken over the management of the Istanbul Park circuit - as saying that an agreement had been reached with Formula One boss Bernie Ecclestone.
Ak said, however, a final decision would rest with the Turkish government.
Turkey joined the F1 calendar in 2005, but was dropped in 2012 following a disagreement with Ecclestone over the fee to stage the race.
Turkish media say Istanbul is likely to fill the July 21 spot that was left vacant on the calendar after the removal of the Grand Prix of America.
Istanbul Park's challenging circuit was popular with many of the drivers. - Sapa -AP
Comment Guidelines
- Please read our comment guidelines.
- Login and register, if you haven' t already.
- Write your comment in the block below and click (Post As)
- Has a comment offended you? Hover your mouse over the comment and wait until a small triangle appears on the right-hand side. Click triangle () and select "Flag as inappropriate". Our moderators will take action if need be.
- Verified email addresses: All users on Independent Media news sites are now required to have a verified email address before being allowed to comment on articles. You are only required to verify your email address once to have full access to commenting on articles. For more information please read our comment guidelines


text:
Knoxville (WVLT) - West Knoxville's the hot spot for sales of the much-abused drug, Oxycontin. Two pharmacies in the Bearden area fill more prescriptions for the pain killer than other in the state.
Pill after pill...
"We do a lot of heart meds. We do a lot of cholesterol meds," Jim Peck says.
Pharmacist Jim Peck fills more than 400 prescriptions a day at Long's Pharmacy.
But when it comes to Oxycontin...
"Not a lot, compared to the total number of prescriptions we run through here," Peck says.
In fact, it's less than one percent. But the Knox County D.A's office says that's not the case in every pharmacy.
"We have a massive problem with Oxycontin and other prescription drugs," John Gill says.
The cost is clear.
"We have almost as many people dying from prescription drug overdoses as we do from homicides in the State of Tennessee," Gill says.
And Gill says two pharmacies in zip code 37919...
That's somewhere here in Bearden...bottle up more oxycontin than any other pharmacy statewide.
"I'm not really sure what that means," Gill says.
Which ones boast the sales?
"That came from law enforcement sensitive sources so I can't name those," Gill continues.
But that's doesn't mean pharmacists are breaking the law.
Even if pharmacists aren't doing anything wrong, and people come to the counter with a prescription, Gill says the number's are still a problem.
"When you've got more people dying from legal drugs than you do illegal drugs, and almost as many are drying from homicides....then you've got a problem," Gill says.
The solution could be a prescription monitoring program system now in the works.
"That's a computer based system to trace and identify problems where it appears people are getting drugs at too many different places," Gill says.
Until then, Peck will keep double counting...and asking customers questions.
"The only way we could do any better would be if we didn't stock it," Peck says.
To keep them safe.
While WVLT allows comments on articles, we ask that you respect the online community. Comments may be removed at any time for violations including:
Comments may be checked for inappropriate content or rule violation, but the station is under no legal obligation to monitor or remove comments. If you believe a comment violates the above rules, please use the Flagging Tool to alert a Moderator.
Flagging does not guarantee removal.

subset: commoncrawl


text:
- Jessa Duggar shares a rare photo of her brother Josh Duggar's daughter Meredith - US Weekly
- Michael Lohan's estranged wife Kate Major is detained by cops at an airport - TMZ
- Is there something between Ben Affleck and Halle Berry? - Gossip Cop
- The Hunger Games author Suzanne Collins says goodbye to the popular series after the final movie is released - Just Jared Jr
- What happened backstage with Kylie Jenner at The Ellen Show? - Radar
- Bryan Cranston tears up over a Breaking Bad memory - Huffington Post
- Leonardo DiCaprio's Oscar race heats up - Lainey Gossip
- The Captain America: Civil War movie may be better than the comic book - The Hollywood Reporter
31 May 2013 at 7:15 AM
Sarah Shahi: Bikini Family Vacation with Shirtless Steve Howey!
About this photo set: Sarah Shahi and Steve Howey spend the afternoon at the beach with their son William while on vacation on Thursday (May 30) in Maui, Hawaii. The 35-year-old Shameless… Read More Here
Photo #: 2881156


text:
Barney Miller: Season 8, Episode 19
Bones (30 Apr. 1982)
A museum presses charges when an Indian lifts his tribe's ancestral bones from an exhibit. A scoutmaster catches a mugger.
Director:Max Gail (as Maxwell Gail)
Writers:Jordan Moffet (teleplay), Nat Mauldin (teleplay), 5 more credits »
Stars:Hal Linden, Max Gail, Ron Glass | See full cast and crew »


text:
6 Common Misconceptions about Laser Hair Removal
We offer Laser Hair Removal in our Corpus Christi office and we are...Read More →
Experience our comprehensive approach to skincare. During your complimentary private consultation, our highly trained and experienced medispa staff will work with you to design a treatment plan customized to fit your unique goals and address your specific skincare needs.
PCA SKIN is well known in the skin health industry for quality education rega…
by appt
Deluxe mineral makeup
Beautifully transform your skin to
look amazing
Join us on Facebook!
Be the first to hear about our specials.
5242 Holly Road
Corpus Christi, TX 78411-4633
(361) 994-7500
9:00 am - 2:00 pm
Tuesday, Wednesday, Friday:
8:30 am - 5:00 pm
Thursday, extended hours:
11:00 am – 7:00 pm
9:00 am - 2:00 pm
Welcome! We are passionately committed to providing you with the least invasive yet scientifically tested, medically approved treatment options.
High-tech MediSpa providing medical aesthetics, Cosmetic services, Advanced skin care technology.
We offer Laser Hair Removal in our Corpus Christi office and we are...Read More →
Laser Hair Removal is a great option for those who have become unha...Read More →


text:
Rollover image for closeup
Takamine Jasmine JO-37 Orchestra-style Acoustic GuitarItem #: MC.JO37-NAT
List Price: $304.99
Unrated ( Be the first to review this item )
by Takamine
Orchestra-style Acoustic Guitar. Made by Takamine. (MC.JO37-NAT)
Free Shipping on this item in orders above $199
Temporarily Out of Stock -- Notify Me when Back in Stock
Found in: 6-String Acoustic Guitars
The Jasmine JO-37 is an elegant orchestra-style guitar that offers refined features and solid-top construction for a sweet, singing sound with great balance and articulation.
For players seeking an enhanced tonal experience, the JO-37 features a select solid-spruce top with Jasmine's Advanced "X" Bracing, and sapele back and sides. The slim neck and short 24 3/4" scale length provide comfortable feel and exceptional playability, making this compact orchestra-style guitar a dream to play.
Other great features include a rosewood fingerboard and bridge, chrome die-cast tuners for great tuning stability, ivory body binding, beautiful abalone rosette, pearl inlays and full gloss Natural finish.
• Solid spruce top with Jasmine Advanced "X" Bracing
• Sapele back and sides
• Rosewood fingerboard and bridge
• Slim neck profile
• 24 3/4" scale length
• Synthetic bone nut and saddle
• Gloss Natural finish


text:
Yitzhak Rabin
A Political Biography
|Publication Date
|January 2014
|Hardcover Ebook (EPUB) Ebook (PDF)
|Palgrave Macmillan
A political, critical, and analytical biography of Yitzhak Rabin, this book makes use of recently-opened archival material and provides explanations for the important episodes in Rabin's life. Leslie Derfler examines Rabin's longtime leadership of the Israeli military and state and Rabin's efforts to secure a peace with Egypt and the Palestinians. He reveals that, while Rabin's opposition to a two-state solution remained firm, Rabin had a longstanding belief in Palestinians' right to shape their own destiny and an underlying awareness of the need for a political rather than military solution to the problem of Israeli security.


text:
Palomar College freshman Ryan Lamb divided his time between defensive end and wide receiver a year ago as a senior at Oceanside High.
Injuries and a bevy of competition caused Lamb to leave his natural position of quarterback. He returned under center this year in his first season with the Comets.
Lamb helped Palomar conclude the campaign on a high note Saturday as he guided the Comets to a 33-28 National Division Southern Conference victory over Grossmont College (5-5).
The 6-foot-4, 215-pound Lamb completed 23 of 50 passes for 295 yards and three touchdowns as Palomar concluded its season at 4-6.
Two of his scoring strikes were 13 yards and 11 yards to Ryan Ena, his former rival from El Camino High. He also hit Anthony Mount from 19 yards out.
Ena reeled in five passes for 106 yards.
"That guy is so athletic," Lamb said of the 6-foot-6, 240-pound freshman, also a basketball standout. "He has deceptive speed and great hands. He'll catch anything he can reach."
Earlier in the season the Comets handed Southwestern its only loss of the year and did not play San Diego Mesa.
"The way we look at it we won the city championship," said Lamb.
The Comets led 33-21 after DeMar Henderson returned a blocked field goal 71 yards with 11:07 to play.
Grossmont scrambled back as quarterback Chris Bonner (22-52, 296, 2 TDs) hit Nickolas Kurtz on a 4-yard scoring pass. It was the second time those two had hooked up in the contest.
Grossmont had two chances to pull out a victory in the final 3:10 but turned the ball over both times in the red zone.
Southwestern 55, Chaffey 25: Visiting Southwestern finished the regular season 9-1 and will host Santa Monica in the American Bowl next Saturday at Olympian High.


text:
Yangon Division What to Pack
Yangon Division Hotels
The Kandawgyi Palace Hotel
Kandawgyi Palace hotel is located on the shore of Royal Lake. I hope these pics will help you to...more
The Governor's Residence
This is a really beautiful hotel fully renovated from an old teak mansion, set in its own gardens. ...more
Traders Hotel Yangon
Location is everything !!! Yes it is, this hotel located in center of city, walking distance to...more
Savoy Hotel Yangon
The hotel was very well maintained, and all the staff were excellant. There were antiques everywhere...more
Parkroyal Yangon
This was where I stayed on my first trip to Yangon. Location wise is pretty good because it is...more
MiCasa Hotel Apartments
If you're planning on staying in Yangon for sometime, it would be a good idea to stay in a service...more
Sedona Hotel Yangon
An international standard 5-star hotel, right at the side of INYA lake. It is 20 mins to the...more
Summit Parkview Hotel
The best thing about this hotel is its wifi service (although with a minimal fee of $2 per hour)...more
Clover City Center Plus Hotel
This is a great budget hotel. It manages to do a few things most budget hotels do not. First – it's...more
Top Yangon Division Hotels
- Rangoon Hotels
- 587 Reviews - 1638 Photos
Instant Answers: Yangon Division
Get an instant answer from local experts and frequent travelers


text: Krome-Pressings Krome Angels Balears PLEASE, DO NOT CONTACT US VIA THIS PROFILE! FOR THIS PURPOSE USE: http://www.myspace.com/kromeangels http://www.myspace.com/kromepressings The backbone for dance innovation, amazing global parties and unquestionable musical talent throughout the last two decades has risen to a new level. Three veterans of the scene who share a mutual respect for each other's music and joined forces back in 2006 to form KromeAngels now deliver their first album. But who are they? This sonic tripartite comprises of Brighton-born Dino Psaras, the first Dragonfly DJ, first release on the Perfecto Fluoro label and member of Cydonia and Ayahusca, Dado, the renowned French music producer who has collaborated with the likes of Bamboo Forest and spearheaded projects such as Transwave, and Shanti, once the young prodigy of the Voov Festival with his infamous baselines and originator of the Visionquest 2003 DVD signature tune. Having identified the need for an alternative from what can arguably been termed as the trance 'norm", these three came together to create a fresh sound, taking the genre to new realms to encompass breakbeat, techno, house and electro and everything in between. A second album will follow as well as a third live album of the best of what has gone before. Look out for them and listen. -------------------------------------------------------------- Krome Pressings is the new home for the KromeAngels' productions, their personal projects and collaborations with like minded music producers. The international sound of the label takes its influence from all music past and present, including trance, electro, acid and progressive house breaking down the boundaries to form a new direction and sound for club trance. Label founders Dino Psaras, Shanti Matkin and Frederic Holyszewski hail from the UK, Holland and France retrospectively. Now based in Ibiza, they bring together the sounds from three of Europe's most influential dance scenes to create the sound of Krome Pressings. First release hot of the press is 'Bounce' a proven favourite from the KromeAngels live show, it features electro heavyweights Product.01 supplying the vocal hooks on top of a stone cold killer synth riff, guaranteed to get crowds jumping worldwide. On the remix is the hot new production team 'Stripper' whose releases on Ministry of Sound, Superstar, Surfer Rosa, Giant Pussy and the legendary Jooris Voorn's Rejected label have established them as one of the names to watch in 2011. The boys with the studio to die for turn out a flawless electro mix with deep-tech influences and devastating breakdowns. Krome-Pressings's tracks Krome Angels - Bounce (Original Mix) by Krome-Pressings published on 2010/12/08 22:17:47 +0000 Krome Angels & Product01 - "Bounce" - Stripper Rmx by Krome-Pressings published on 2010/12/07 03:46:31 +0000


text:
keeping it Real
This is funny, but if a white guy was doing this skit saying black people need a black president, there would be outrage, people saying he was racist.
By the way 72% of the American population is White, only 12% are black. This means more white people voted for Obama than black people.
By the way being racist is not a crime, but people are trying to turn it into that.
The constitution states FREEDOM OF SPEECH, so Chris can do this skit, I love Chris Rock, and think this skit is funny, but just want to point out how Black people have more Freedom than White people, in 2012. Taking White people's rights away does not make it right, with what happened with white on black racism years ago. Now, there is more Black on White Racism, and I know cause I used to date a black girl, and when we walked arm in arm in white neighborhood people said hello, in the black neighborhoods, they would call her MOTHERF****KIN WHITE BITCH.
SOCIETY NEEDS TO GET A GRIP. As long as black people and the media make race an issue, it will always be an issue. Racism will never end and you can't make people change their beliefs, Racism is wrong, but people have the freedom to believe what they want, and if you don't think so then you are wanting USA to be a communist country.


text:
cc'); } -->
MEDICAL BILLING SKILLS IN DEMAND! Become a Medical Office Assistant! NO EXPERIENCE NEEDED! Online training can get you job ready! HS Diploma/GED & PC/Internet needed! 1-888-528-5547More info Email a friend
CNA, LPN, RN, Classes CPR, Med tech, Phlebotomy www.flacna.com 941-727-2273More info Email a friend
Need a job?
Need to hire some help?


text:
Looking to pick up a pair of lights for my teenage son for Christmas. I can't afford to go the Lupine route and my LBS is willing to make me a screaming deal on a combination of the NiteRider Pro 1200 Race and/or 1800 Race. So which is the best combo? 1200/helmet with 1800/bar or simply a pair of 1800's?
Results 1 to 4 of 4
Similar Threads
Hot Deals See All Hot Deals >>
Latest Mountain Bike Articles


text:
Mike McCosker always feared that his having a heart attack was a foregone conclusion. There is a history of heart trouble in his family, including his father, who has survived two attacks. Still, it came as a shock when it actually happened since he was only 44 years old. "There are so many emotions that […]
Archive | Patient Experience RSS feed for this section
You are here: Home > Patient Experience
Never Too Young: Mike McCosker's story
April 29, 2015 in Patient Experience
A Commitment to Healing
March 25, 2014 in Patient Experience
When Jim Leonard came to the Brattleboro Memorial Hospital Center for Wound Healing last summer, he was ready to try anything to treat the deep, open wound on his left foot. It had been about two years since he had an operation to remove an infection. His weekly follow-up appointments with his doctor consisted of […]
Jane Rosser and Ken Vitale
October 25, 2013 in Patient Experience
Jane Rosser came to Brattleboro in the 1970s as the means to a beginning rather than an end. The School for International Training provided her with a master's degree and the opportunity to work abroad; initially in Kenya, then other countries and continents. She met Ken Vitale in between assignments during the early 1980s when […]


text:
Star Trek: Full Speed Ahead
View Single Post
February 1 2008, 03:39 AM
First Faster Than Light
Location: Silicon Valley, CA, USA
Re: Star Trek: Full Speed Ahead
All right, here's Act I:
NCC-60597/03 (Shuttle Ptolemy)
Spaceport Two, Antares Ship Yards
Stardate 43222.03
Captain Krystine Leone stepped in from the shuttle's passenger compartment. It was one of the type-seven shuttlecraft in use by Starfleet; with its curvaceous exterior, it reminded her of a reverse-colored panda bear. She ran a hand across the line of the console in front of her and tapped in a few commands to check on the shuttle's status. The yardmaster promised her a pilot, but she had yet to see him or her arrive, yet.
The Ptolemy was to be one of forty-seven auxiliary craft assigned to her ship, and just being inside it made her feel giddy at the prospect of assuming command of a Nebula-class starship. The shuttle felt like an extension of her impending status as the newest starship captain in the United Federation of Planets.
She stood from her seat and rechecked her stowed gear in the shuttle's storage area underneath the portside bench seat in the rear compartment. Satisfied, she turned to go back to the cockpit when she heard the familiar click of uniform boots on the shuttle's deck. Her eyes looked down at the shuttle's chronometer, counting away the seconds upon the corner display of the console.
The clicking stopped, just behind her. She turned her head, ready to introduce herself to the pilot, when she realized that she did not have to.
"Wilson," she smiled.
Lieutenant Wilson Nieves returned the smile, the skin around his brown eyes wrinkling as he did so. "Congratulations on your promotion, Captain."
She beamed, "Thank you! How are you?"
He settled into the pilot's seat and activated the console. "I'm doing fine, sir."
"Last time I saw you, you were high-tailing it off the Potemkin. I heard you went home."
"I spent a few weeks on leave," he nodded. "But then I heard they promoted you and were giving you a ship. So, I decided to cut my leave short so I could at least see you off."
She winced, inwardly. He applied to be her new chief tactical and security officer, but he lost to a more qualified officer by the name of Daniel Hunter. "I'm sorry you didn't get the berth, Wilson. I really am."
Nieves waved her off with a shake of his head. "No, it's all right, sir. You went with the right man. Danny's going to make you proud." He turned his head, after signaling for launch clearance. "And besides, you've got Commander Elannis, too. So, you're definitely in good hands."
Leone grinned. Ariel Elannis, Wilson, and she had all served together on the Excelsior-class starship USS Potemkin, under the command of Captain T'Cirya. When T'Cirya received her promotion to rear admiral, the crew dispersed to different ships in the fleet, as Potemkin's new captain began to assemble his new senior staff. "I know she's already here."
"She's been here for weeks. She and your chief engineer have been making use of the time during the final stages of the refit," he informed her. "Control, this is Ptolemy. Engaging thrusters for launch."
The shuttlecraft shuddered slightly as the primary thrusters lifted it off of the deck and pass the energy barrier holding the atmosphere inside. They slipped into space from the launch bay.
"Petra Bartlet. I haven't had much of a chance to meet everyone... except Commander Kincaid."
"Your new first officer?"
"I didn't see him on board."
"He's still at Starbase Three-One-Zero, waiting for us to pick him up. Actually, most of the crew are there."
Nieves asked, "Because of the warp tests, right?"
She nodded. Farragut's refit included an upgrade to the warp's core and field coils. It required a warp test to tune the coils to the new core and that would fall to Lieutenant Bartlet and her entire division. In order to accomplish this, they'll need to take the ship out away from any stellar body and engage the engines. "We'll only have a hundred seventy-four in crew, including the civilian engineers who'll observe and consult. You know, the new core was originally intended for the Sutherland."
"You say that with such pride, sir."
"Are you making fun of me, Lieutenant?"
Nieves smirked, but forced a dispassionate expression onto his face. "No, sir, Captain, sir!"
Unable keep a straight face for very long, both dissolved into laughter. Leone placed a hand on his shoulder and smiled. "I'm glad you were able to make it to the ceremony."
"I wouldn't have missed it for anything, sir." Under his command, the shuttle banked around the marker beacon for Yard Thirty-Nine-Alpha, where Farragut was dry-docked. The bow of the shuttle swung into view and he knew it would be a special moment. "There she is, sir."
Leone looked away from him and out to the scene before them. Cradled within the span of the drydock facility was NCC-60597. From their vantage, she could see underneath the Nebula-class heavy cruiser's saucer, which was an exact duplicate of the saucer one would find on a Galaxy-class starship. She breathed, "Oh, my gods..."
He expected her response. "Beautiful?"
"She looks... cold."
Nieves looked at the ship again. None of its lights were on, including the warp nacelles or the navigational deflector. "They're keeping her on internal power, right now. They already did the power system tests-"
"Two days ago," she interrupted him. "I've been keeping up with the reports."
He grinned. "Yes, sir."
"Can you take us under the saucer and then over?"
"Of course."
The shuttle dived below the 'waterline' of the saucer section and the large block numbers of the ship's naval construction contract number were as big as her eyes. Pride surged within her as she reached out with her hand to touch the viewport plexiglass, as though her fingers might pass through so she could touch the hull with her bare hand. When her fingers hit the glass, she pulled them back quickly and gave Wilson a sheepish grin.
The underside slowly crawled by the shuttle. They continued on and she noticed a big divot at the very bottom. "Hey, where's my gig?"
Wilson looked at the docking port for the missing captain's yacht and shook his head. "I don't know, sir. Maybe it'll be delivered later?"
She frowned. "Damn. I wanted to take a look at her."
"Did you have a name picked out?"
"No. I was hoping I would be inspired, later."
"Would you like me to find out where it is?"
Leone shook her head. "No, I'm sure I'll find out once I'm aboard."
"Aye, sir." When they reached the navigational deflector, he announced, "I'm bringing her about, now." They moved along the port side of the saucer and floated up and over the lip.
The upper half looked more impressive than the bottom. Ptolemy approached the ship's bridge module and she looked closely at the viewports. "There's my ready room," she grinned uncontrollably.
"Yes, sir."
The shuttle's speakers crackled to life. A woman with a contralto voice called to them, "This is Farragut Control to inbound shuttle. Please identify yourself. You are entering the approach and landing for our main shuttlebay."
He tapped at his console and spoke, "Control, this is Shuttle Three, Ptolemy, on approach. We are inbound from Antares Central."
"Your landing code, please."
"Code Six-Farragut." The code announced to control that a ranked captain was on board, and additionally, that captain was their new commanding officer. "Request landing clearance at your convenience."
A different voice replied; a deep male baritone. "Code Six-Farragut acknowledged. Welcome, Captain Leone. Commander Tennyson sends his respects."
Leone spoke up. "This is Captain Leone. My compliments to the commander."
"Ptolemy, you are cleared to land. Approach vector alpha."
Wilson nodded. "Approach vector alpha, confirmed. On final approach, now."
The cavernous main shuttlebay looked more like a fully-fledged planetside spaceport than its name implied. Shuttles and pods of varying sizes moved within the bay and out, while other shuttles approached and landed on one of the four light strips available for them to do so. Ptolemy approached on the port-side strip and then guided to a waiting compartment where a staff of flight deck personnel burst forth to tend to the shuttle.
Leone smiled. "Smooth landing. Thanks for taking me over, Wilson."
"Anytime, sir," replied Nieves with a smile of his own. "I'll take care of post-flight. Why don't you look around and get familiar with your new digs?"
Even if he hadn't mentioned it, she would've done so. "I will. See you later?"
"I'll be here for the ceremony, so definitely, sir."
"Excellent." She didn't wait another moment, instead gathering her gear and making for the exit.
"Captain Leone, I presume?" asked the stocky dark-skinned man in the gold Commander's uniform.
"You presume correctly, Commander Tennyson."
He smiled and extended his hand. "Commander Robert Tennyson, sir. A pleasure to meet you."
"I feel like I know you, already, Commander," she replied, accepting the firm and warm handshake. Since the Farragut arrived at the Antares Ship Yards for its five-year refit nearly eight months ago, Commander Tennyson assumed engineering command of the ship. "I've read every one of your reports going back to the beginning of the refit."
"Then you have me at a disadvantage, sir," he said as they ended the handshake. "But I do know that congratulations are in order, Captain."
"Thank you. Do you prefer Robert or Bob?"
"Rob, actually."
Leone smiled and asked, "Rob, where are you bunking me?"
"Deck eight, section alpha one."
"Nice. Is it ready?"
Tennyson grinned. "In anticipation of your arrival today."
"Then, lead on."
"Aye, sir." He barked, "Yeoman, get the Captain's gear and follow us, please."
A yeoman third class moved quickly and offered to carry her duffel and garment bag. She relinquished both to the young enlisted petty officer and followed behind the broad back of Rob Tennyson.
---- Scene Change ----
Saleb finished installing the device and tucked the isolinear connection behind the bulk of the control computer. He began entering in commands on the scanner, satisfied with the response and looked up.
Leaning over the rail, Belkis shot an expectant look at him. "Well?"
"It's ready."
"Are you sure?"
Saleb looked back down at his scanner. "I think so."
"You think?"
"Look, I've only done this once before."
"Gods, help me..."
"Do you want to come down here and do it?"
Belkis raised both hands in dismissal. "No, no." He sighed. "When we activate it, what's going to happen?"
"One of two things, I guess. Either, it'll work and we'll have succeeded..."
"Or it'll destroy the navigational deflector and possibly the entire bottom half of the ship."
Saleb ran a few more calculations and then snapped his head back up. "No, wait."
Belkis perked up. "Yes?"
"I was wrong."
He let out a sigh of relief. "Oh, good."
"Both could happen. We could succeed and then blow up."
Belkis placed his hand over his face and closed his eyes. "I should've stayed home."
And there's Act I. The whole thing is only 9k in word count, so the acts are a little short, but this first episode is a multi-parter.
Thanks for reading.
Michael D. Garcia
Head Writer, Star Trek: Full Speed Ahead (
United Trek
my writing blog
my fanfic profile at Ad Astra
View Public Profile
Send a private message to Zefram_Cochrane
Visit Zefram_Cochrane's homepage!
Find More Posts by Zefram_Cochrane


text: Warning: Declaration of vB_ProfileBlock_vBSEOLikes::block_is_enabled() should be compatible with that of vB_ProfileBlock::block_is_enabled() in Unknown on line 0 View Profile: edrex - MP3Car.com Register Help Remember Me? Blog Technology Events Products and Technology How To Videos mp3Car News Product Reviews Store Sales Write a blog Auto App Mart Store Shop Store Sales Contact Store Survey New Vendor Signup Forum Forum Home Contact Projects Admin Mod Log In Enterprise Free Phone Consultation What We Do Partners Contact About Us History Team Press Release Contact Resources wiki FAQ Videos How to Use the Forums CarPC 101 Videos Videos mp3Car Today's Posts Community Groups Albums Member List Forum Actions Mark Forums Read Quick Links View Site Leaders New Posts Live Forum Rules Advanced Search Member List edrex under nav bar edrex FLAC Array Find latest posts Find latest started threads View Articles View Blog Entries Likes Received (0) Likes Given (0) Mini Statistics Join Date 12-03-2004 Last Activity 08-17-2008 07:02 AM Avatar Recent Visitors The last 10 visitor(s) to this page were: drug_online, durwood, ecog, Enforcer, Kalt, mazda3owner13, Pills_online, Quattro, StephJones, Tilley This page has had 1,606 visits Tab Content About Me My PhotosLikes Given About edrex Basic Information Date of Birth May 12, 1979 (36) About edrex Car Details: 2005 Nissan Altima Status: Eaten by Cannibals Ranking: Mr. 20,000!! Location: West Chestinghamshire, UK init!! Signature Spent so far: $1,755 Check Out My Work Log for Specs/Pics CarPC Progress[!!!!!!!!!!!!!!].....Installation[---------+].....Pastrami Progress[!!!!!!!!!!!!!!] Don't mess with Texas. It's not nice to pick on retards! Statistics Total Posts Total Posts 1,088 Posts Per Day 0.27 General Information Last Activity 08-17-2008 07:02 AM Join Date 12-03-2004 View All Images My Photos No image hosted 0 Likes -- Default Style -- Default Mobile Style Contact Us mp3Car Forums Archive Top All times are GMT -5. The time now is 06:08 PM. Powered by vBulletin® Version 4.2.2 Copyright © 2015 vBulletin Solutions, Inc. All rights reserved. Content Relevant URLs by vBSEO 3.6.0 PL2 Copyright © 1999 - 2011 Mp3Car.com Inc.


text:
User Stats
Profile Images
User Bio
Marhaba (Welcome) to the new Qatar Motor and Motorcycle Federation and Losail International Circuit video contents website which we hope you find easy to navigate.
This is an exciting time for QMMF and LIC, the home of motorsport in Qatar. Our highly interactive website has many new features, including regular motorsport news updates, hot motorsport topics and new trends, and it is a great way for grassroots fans and professionals to stay informed about motor sports in Qatar.
Be sure to come back regularly to discover the latests news and what's happening at Qatar Motor and Motorcycle Federation and Losail International Circuit.
External Links
Related RSS Feeds
Recently Uploaded+ See all 202 videos
Recent Activity
Shaun Wilson46 subscribed to 2013 Losail Motocross Championship
Bike Middle East followed QMMF TV
ابراهيم الهيال subscribed to QCH Qatar Challenge


text:
Claudia Hiersche
Born Feb 22, 1977
Age 38
Hometown Frankfurt (Oder)
Other Names Claudia Hiersche
Claudia Hiersche (* 22 February 1977 in Frankfurt, German Democratic Republic is a German host and actress and is well known for her portrayal of the lesbian fictional character Carla von Lahnstein in the soap opera Verbotene Liebe, where she was on contract… Read More
View newly released photos of Claudia Hiersche.
News + Updates
Browse recent news and stories about Claudia Hiersche.
Gntm Gewinnt Quotenschlacht: Heidi Klum überzeugt Werberelevante Zielgruppe Stern.DeGoogle News - May 27, 2011
Claudia Hiersche: Diese Frau Ist Eine Waffe BildGoogle News - May 25, 2011
Rtl Hauptrolle Für Überlinger Schauspieler Suedkurier.DeGoogle News - May 24, 2011
Claudia Hiersche: Vorsprechen Im Eigenen Wohnzimmer Lübecker NachrichtenGoogle News - May 16, 2011
Learn about the memorable moments in the evolution of Claudia Hiersche.
1977 Birth Born on February 22, 1977.
2000 23 Years Old She attended a drama school in Manhattan for three months. Before her work on Verbotene Liebe, she was the host of the travel magazine television show Wolkenlos from 2000 to 2002. … Read More
2006 29 Years Old As for her character in Verbotene Liebe she became very popular, when her character Carla had a relationship with Hanna Novak. In 2006, Carla was the first lesbian character on German soap opera that got married to another woman. … Read More
Original Authors of this text are noted on http://en.wikipedia.org/wiki/Claudia_Hiersche.
Text is made available under the Creative Commons Attribution-ShareAlike License.
Text is made available under the Creative Commons Attribution-ShareAlike License.


text:
Jaycee P.
Interests: I'm just a big kid myself. I am a German car enthusiast. I love riding four wheelers, playing sports (soccer especially), being outdoors, and spending time with friends and family.
Experience: I used to babysit my four cousins on a regular basis for about 2-3 years. They were all under the age of 7 at the time, two of them being a set of infant twin girls. I have babysat several different children (ages 2-11) over the years on various occasions. This included a job where I was hired as a role model, soccer trainer, babysitter for the summer of 2008 for an 11-year-old girl. I was employed by soccer team where I coached youth soccer players, ages 5-9. I constructed and directed activities to help develop and improve skills while evoking fun in learning.


text:
Photos: Ranking every NFL team's draft from 1 to 32; where do the Cowboys fall?
Photo: Kevin C. Cox/Getty Images
21. Buffalo Bills: GM Buddy Nix picked up a pair of promising corners (Stephon Gilmore, pictured, Ron Brooks) and offensive linemen (Cordy Glenn, Zebrie Sanders), allowing them to upgrade talent while filling cracks. This team is inexorably moving toward snapping its league-high 13-year playoff drought.
To post a comment, log into your chosen social network and then add your comment below. Your comments are subject to our Terms of Service and the privacy policy and terms of service of your social network. If you do not want to comment with a social network, please consider writing a letter to the editor.


text:
about Todd FlowerdayA Roman Catholic lay person, married (since 1996), with one adopted child (since 2001). I serve a parish in music ministry.
about John Donaghy
John is a lay missionary since 2007 with a parish in western Honduras. Before that he served in campus ministry and social justice ministry in Iowa. His ministry blog is http://hermanojuancito.blogspot.com
He also blogs reflections on the lectionary and saints/heroes/events of the date at http://walktheway.wordpress.com
He'll be a long-term contributor here analyzing the Latin American bishops' document from their 2007 Aparecida Conference.
Bible Readings
Vatican II pages
Recent Posts
Recent Comments
Devin Rice on Apple or Pumpkin? Liam on Songleader to Choir Liam on Apple or Pumpkin? Liam on Apple or Pumpkin? Liam on The Warm-Up Liam on The Warm-Up Liam on Acclamation or Call/Response? Jim McCrea on Acclamation or Call/Response? Mary on The Coming Mercy Atheist Max on Laudato Si 162: Decline in Eth…
- … deep in your soul
- A Concord Pastor Comments
- dotCommonweal
- Dyspeptic Mutterings
- Fr Richmond
- Gentle Reign
- Gotta Sing Gotta Pray
- Hermano Juancito
- In All Things
- Liturgy & Catechesis Shall Kiss
- More Meredith Gould
- Optima Musica Dei Donum
- Perspective
- PrayTell
- The Parish Blog of St. Edward the Confessor
- The Sacred Page
- The Steeplechase
- There Will Be Bread
- Universe Today
- Walk The Way
- WordPress.com
- WordPress.org
Interesting sites
Blog Stats
- 5,773,260 hits
Daily Archives: 26 November 2011
Enough Loss For A Lent
Or maybe a few Lents. And it's not even 2012 yet, let alone February. People have commented on the difficulty of dieting the past several months. I don't know what to tell them. While I love certain foods, especially sweets, … Continue reading
Posted in Food, My Family, Parish Life 3 Comments
Retired Books
In addition for the preparations for a new liturgical year, it's also a cleaning-out day in the sacristy. Cycle A lectionary: to my office. Cycle B: bookmarked and in place. Gospel book gets its ribbon moved back to the start. … Continue reading
Posted in Liturgy, Parish Life 1 Comment
GDC 69: "Continuing education in faith within the Christian community"
Today we get a new topic, ongoing faith formation, covered in GDC 69-72. Language is more than political lingo, and I think there are good reasons to avoid "education" where "faith formation" is a more descriptive term. The heading for … Continue reading
GIRM 46: Introductory Rites
Today we begin a long section of the GIRM (numbered sections 46 through 90) that reviews and legislates for the individual parts of the Mass. It's important to remember these sections do not exhaust what the Church teaches on the … Continue reading
Posted in GIRM, post-conciliar liturgy documents Leave a comment


text:
My Nonprofit Reviews
300 profile views
1 reviews
Review for Chiara Home, Inc., South Bend, IN, USA
We have utilized Chiara Home since 2004. At the time we had no respite. Sister Gretchen, Brenda and the staff have been such a blessing to our family. Chiara Home is also a connection for other Disability resources. This past Saturday we took our daughter to a Fall O Ween Party that was e-mailed to us as an upcoming event for Special Needs Children.
Role: Client Served & We received Respite for our daughter, and other Special Needs events.


text:
The National Fish Habitat Conservation Act, S. 2080, was introduced on March 5, by Sens. Benjamin Cardin (D-MD) and Mike Crapo (R-ID). This vital piece of
legislation would strengthen a program that has been in place for close to a
decade and contributes to river rehabilitations, reservoir enhancements,
salt-marsh protection efforts and other fishery conservation projects across
the country.
Fish habitat resources are of enormous significance to the
economy of the United States providing recreation for 60 million anglers; more
than 828,000 jobs and approximately $115 billion in economic impact each year
relating to recreational fishing; and 575,000 jobs.
Healthy habitat = healthy fish populations = better fishing!
Please urge your Senators to
become cosponsors of this important bill.
If you take action, you will receive periodic updates and communications from KeepAmericaFishing and the American Sportfishing Association.
Please Support S. 2080, the National Fish Habitat Conservation Act
Dear [Decision Maker],
As your constituent and an angler who supports fisheries conservation, I urge you to cosponsor the National Fish Habitat Conservation Act (S. 2080).Our state is fortunate to have many important fishery resources that are a vital part of our quality of life. Overall, recreational fishing has a $115 billion impact on our nation's economy annually, contributing over $11 billion in state and local taxes. The National Fish Habitat Conservation Act will, for the first time, establish a national framework for freshwater and coastal habitat conservation, restoration and enhancement.
Sincerely,[Your Name] [Your Address] [City, State ZIP]


text:
Rollover image for closeup
More Movie and TV SongsItem #: HL.02500304
Our Price:
Unrated ( Be the first to review this item )
Composed by Various.
Just for Kids - Not!. Big Note. Easy piano arrangements with large, easy to read notation. Piano Level: Easy. 9x12 inches. 40 pages. Published by Cherry Lane Music. (HL.02500304)
Free Shipping on this item in orders above $199
Temporarily Out of Stock -- Notify Me when Back in Stock
Found in: Easy Movies/TV Piano Sheet Music
Features 11 songs from Fantasia, Cinderella, Sesame Street, The Prince of Egypt and other kids' favorites arranged to be played in two ways: as solos in easy-to-play big-note notation, and as duets with accompaniments to be played by a teacher or parent. Includes: Chicken Run Main Theme · Dance of the Sugar Plum Fairy · In My Own Little Corner · Pokémon Theme · Rubber Duckie · Sing · When You Believe · and more!
Songs Include:


text: Has anyone experienced glitches or problems playing Madden? I was disgusted by NCAA this year but I gave Madden a chance and I love the game but I experienced freezing and glitches like no other. The freezing occurs only when I play a season and two glitches I experienced was with the Steelers on "Hardcore" Mode playing the Browns in a season. I challenged a play and the challenge I wanted wasn't what I was challenging in the first place and they ended up moving the ball back 5-7 yards and giving me 3rd an Inches! So that was a goofy glitch there, and then my FB (Davis) was flying in the air and I couldn't hike the ball, the only thing I could do is special team plays. So I kicked a field goal and thought "Maybe this will fix things." but when I was on defense my LB (Harrison) was flying up and down on the ground bouncing all over lol. I recorded these on my phone and I am going to post them YouTube in a few days to prove it.


text:
1. Left drag the objects pointed by red arrows in the following diagram to alter their positions.
2. Right click (or control-click) anywhere and then drag the whole system to move.
3. The beam is capable to be turned to any, but not a vertical position.
4. The masses of the beam and the spring balance are neglected.
5. If the beam "collapses" unfortunately, reset the page and start again
About torque


text:
Copper Flats
13711 East Richthofen Circle, Aurora, CO
Are you the manager of this property?Click here to claim this listing Check Your Credit Now
About Copper Flats
At Copper Flats we are dedicated to providing you with the best in living experiences. With that goal in mind, we have furnished each of our homes with the highest quality stainless steel appliances for an up to date, polished look. The first stylish aspect you will notice upon entering the home is the beautiful wood-style flooring placed throughout the common areas. Our wood-style flooring is only matched by the dark wood cabinetry. Weve placed a washer and dryer in each home, so you never have to worry about sitting around at a laundromat to clean your clothes. Our homes are fitted with walk-in closets to give you plenty of room for all of your clothing and storage needs. When thinking about comfort, weve placed carpet in the bedrooms of the homes for that extra sense of relaxation. And after a long day, step out onto your very own balcony or patio to enjoy the beautiful views of the surrounding area.
As you search for your perfect apartment, please keep in mind that rental rates (including specials and discounts) and availability are very fluid in the apartment industry. Based on many factors, the rental rates (including specials and discounts) and availability can actually change not just daily, but hourly, if not even more quickly. While our advertisers do take steps to keep their information current, the rental rates or availability you may find here now at ForRent.com may not be the same as when you contact the communities.
Here are some similar properties you might be interested in
Aurora Meadows
Aurora, CO
From $644
Studio - 3 Beds
Aspenwood Apartments
Aurora, CO
From $935
1 - 2 Beds
21 Fitzsimons Apartment Homes
Aurora, CO
From $1121
Studio - 3 Beds
Elk Ridge
Aurora, CO
From $900
1 - 3 Beds


text:
|City of license
|Boston, Massachusetts
|Broadcast area
|Greater Boston
|Hot 96.9
96.9 Irish(HD2)
|Boston's New #1 for Today's Hottest Music & All the Best Throwbacks (Analog/HD1)
Boston's Irish HD Station (HD2)
|96.9 MHz (also on HD Radio)
|First air date
|Rhythmic Top 40
HD2: All Irish music
|22,500 watts
|224 meters (735 ft)
|Facility ID
|Transmitter coordinates
|42°20′49″N 71°04′59″W / 42.347°N 71.083°WCoordinates: 42°20′49″N 71°04′59″W / 42.347°N 71.083°W
|Former callsigns
|Greater Media
(Greater Boston Radio, Inc.)
|Sister stations
|Listen Live (requires login) or Listen via iHeart
HD2: Listen Live
HD2: www
WBQT is a commercial radio station serving the Boston area on 96.9 FM, currently airing an urban-leaning Rhythmic contemporary format, branded as "Hot 96.9". Its studios are located in Dorchester and broadcasts from a transmitter atop the Prudential Tower in Boston's Back Bay neighborhood.
The station originated in 1945 as W1XHR (later WXHR), owned by Harvey Radio Laboratories and programmed a classical music format. In 1966, WXHR was sold to a joint venture of Kaiser Broadcasting and the Boston Globe and in 1967, became beautiful music station WJIB (whose AM successor operates out of the old Harvey Radio Labs building in Cambridge). After several further ownership changes — first to General Electric in 1972, then to NBC in 1983 (three years before the merger between NBC's parent company, RCA, and GE) and Emmis Communications in 1988 — it flipped to smooth jazz as WCDJ, "CD96.9", beginning in 1990. After Greater Media bought the station in May 1993, the station began stunting with a simulcast of new sister station WMJX at 1 PM on May 4 of that year. 2 days later, at 4 PM, WCDJ flipped to country as WBCS. The station became WKLB-FM in August 1996 after the previous WKLB-FM was bought by Greater Media and consolidated with WBCS, with its frequency being converted to WROR-FM. Smooth jazz returned at Noon on August 22, 1997 as WSJZ, after a format swap with what had been WOAZ (now WCRB); in September 1999, it flipped to Talk, completing a 4-month transition to the format. Shortly after the flip, the station changed its call letters to WTKK. During its tenure as a talk station, WTKK used several monikers: "96.9 FM Talk" from its 1999 launch until 2007, "96.9 WTKK: Boston's Talk Evolution" from 2007 until 2010, "96.9 Boston Talks" from 2010 until September 2012, and "News Talk 96.9" from September 2012 until its January 2013 demise.
In April 2007, WTKK management attempted to add Boston Herald columnist and talk-show host Howie Carr to its lineup, after the station's syndicated morning show Imus in the Morning was cancelled after Don Imus' comments about the Rutgers University Women's Basketball team got him fired by CBS. But Carr's long-time radio-station employer, Entercom-owned WRKO, blocked that move by exercising a clause in Mr. Carr's contract allowing it to make a matching counter-offer.[1] Carr held out until November 16, 2007, after which point Carr returned to WRKO. On December 3, WTKK resumed broadcasting Imus in the Morning when it was picked up by WABC, but over time, was locally limited in its broadcast time, ultimately only airing for 2 hours every weekday morning.[2] In January 2011, WTKK dropped Imus from their lineup completely in favor of an extended edition of their late morning team of Jim Braude and Margery Eagan.[3]
WTKK fired the controversial Jay Severin, a host since 1999, in April 2011 after he said he had slept with female interns at a company he had owned, and defended the practice.[4] He was officially replaced in June 2011 by radio host Doug Meehan.[5] Two months later, when rival station WXKS hired Severin for afternoon drive, WTKK shuffled its lineup to place politics-heavy Michael Graham in the 3 p.m. slot. The lineup at this point included Eagan and Braude, followed by various hosts in late mornings, then syndicated talker Michael Smerconish; an open slot, followed by "The Daily Wrap", hosted by Michael Castner and syndicated by the Wall Street Journal Radio Network, then the syndicated John Batchelor and Overnight America shows.
End of Talk and the launch of Hot 96.9[edit]
In December 2012, Greater Media registered several web domains for WTKK, pointing that the future of the news/talk format was in serious doubt due to low ratings (in its last book as a talk station, WTKK had a 1.9 share of the market in the Boston Arbitron radio market ratings). Many of the domains included the word "Beat", pointing to an urban contemporary, Rhythmic CHR or Rhythmic AC format; if this were to occur, this would have marked a return for either the Rhythmic AC format (which was last heard in 2005, when WQSX flipped to adult hits) or the urban format (which was last carried on a Boston station in 2006, when the former WILD-FM flipped to a simulcast of WAAF) in the market; in either case, this would have caused the station to compete against long-dominant WJMN.[6] Other domains pointed to possible adult hits, oldies, all-news, sports talk, active rock, dance music, adult contemporary, classic rock, mainstream rock, alternative rock, adult album alternative, or CHR formats. Further fueling the rumors of a format flip, on December 17, 2012, morning host Doug Meehan left to pursue a television opportunity in Arizona, while afternoon host Michael Graham left when his contract was not renewed.[7] In addition, weekend hosts were told the last weekend of 2012 that their shows would not continue in the new year.
In compliance with a press release by Greater Media on January 1, the station flipped at 10:00am on January 2 after Jim Braude and Margery Eagan's morning show, starting its new incarnation as Urban Contemporary, "Power 96.9", beginning with "Diamonds" by Rihanna.[8] However, this would only turn out to be a stunt; at 10:00am the following day, the station shifted to Dance, branded as "Nova 96.9."[9] At Noon on January 4, the stunting shifted to adult hits, branded as "96.9 Mike FM" (using the former moniker/format of WMKK). WTKK shifted to classic rock at midnight on January 6, as "96.9 The Bone."
On January 8, at 11:00am, the station debuted its new, permanent format: Rhythmic AC, with the branding "Hot 96.9"; "Run This Town" by Jay-Z was the first song played. According to a press release from Greater Media, the station's direction will primarily be focused on current Rhythmic and Dance hits mixed in with recurrents from the 1980s and 1990s, as it targets an audience who grew up listening to WJMN in the 1990s and 2000s, but want an alternative to Adult Top 40 WBMX, whose presentation is more Pop/Rock oriented. The flip brings the format back to Boston for the first time since WQSX flipped to WMKK in 2005. In addition, like WQSX, WTKK is also using "The Rhythm of Boston" as its on air slogan (albeit briefly; the station adopted its "Today's Rhythm & All the Best Throwbacks" slogan a month later, which was modified to "Today's Hottest Music..." in July 2014).[10] On March 20, 2013, WTKK changed its call sign to WBQT. Clear Channel soon obtained the former call sign for use on their current 106.1 FM facility in Raleigh, North Carolina. Since the flip, the station's ratings have improved, with WBQT currently ranked 11th, with a 4.0 share of the market.
In terms of staff announcements, Greater Media confirmed on January 3 that Pebbles, former long-time morning co-host on WJMN, will be WTKK's new morning show host.[11] Also brought on from WJMN was Melissa, who is the afternoon drive time host from 2PM to 7PM. In addition, on January 4, Greater Media announced that Jackson "Cadillac Jack" McCartney has been hired as Director of Programming for all 5 stations in their Boston cluster.[12] On February 20, it was announced that former WJMN DJ Roy Barboza was named Music Director and Mix Show Coordinator.[13] On March 1, it was announced that Baltazar, who co-hosted WJMN's morning show with Pebbles from 1995-2001, would become the new co-host of Hot 96.9's morning show beginning on March 4, reuniting him with Pebbles.[14] In August 2013, former WJMN personalities Diedre Dagata and Hustle Simmons became weekend hosts at the station. On January 8, 2014, former WXKS personality Jackson Blue became WBQT's new afternoon host, with Melissa moving to middays. In April 2014, former WJMN personality Bobby Blaze briefly became a Sunday afternoon/evening host (he would be replaced by Reggie Beas). On August 18, 2014, Baltazar was released from the station, and was replaced by former New England Patriots player and WBZ-FM host Jermaine Wiggins; in addition, Melissa moved to morning drive, with Dagata replacing her in middays.
With the station having moved further to Rhythmic Top 40, and in effect on par with WJMN, Mediabase has moved the station to the Rhythmic panel in January 2015.
WBQT-HD2 programs an all-Irish music format as "96.9 Irish", due in part to Boston's Irish connections and background. The HD2 subchannel has been on-air since 2006, when it debuted as "Classical 2.0." In late 2006, when Greater Media purchased classical station WCRB, 96.9-2 flipped to their current all-Irish format.[15]
- ^ http://www.boston.com/business/globe/articles/2007/09/20/ruling_puts_carr_in_limbo_between_two_stations/
- ^ http://www.bostonherald.com/business/media/view.bg?articleid=1201852 WTKK dials back Don Imus, tunes into local talent
- ^ http://www.bostonherald.com/jobfind/news/media/view/20110112wtkk_kicks_imus_out_adds_more_of_eagan/srvc=home&position=also WTKK kicks Imus out, adds more of Eagan
- ^ http://www.boston.com/ae/tv/articles/2011/04/07/contentious_talk_show_host_severin_fired/?rss_id=Boston+Globe+--+Today%27s+paper+A+to+Z Contentious Talk Show Host Severin Fired
- ^ by http://bostonherald.com/blogs/news/messenger/index.php/2011/06/14/its-official-meehan-replace-severin/
- ^ http://radioinsight.com/blog/headlines/netgnomes/79932/whos-dropping-a-beat-into-boston/
- ^ http://www.allaccess.com/net-news/archive/story/113639/michael-graham-exits-wtkk Michael Graham Exits WTKK
- ^ http://www.greatermedia.com/?p=4303
- ^ All Access Music Group (January 3, 2013). "Power 96.9 Now Stunting As Nova 96.9 In Boston". All Access Music Group. Retrieved January 3, 2013.
- ^ Lance Venta (January 8, 2013). "Hot 96.9 Boston Debuts". Radio Insight. Retrieved January 8, 2013.
- ^ http://www.greatermedia.com/?p=4321 Veteran Boston Morning Personality Pebbles Joins 96.9FM
- ^ http://www.greatermedia.com/?p=4328 Cadillac Jack Named Director of Programming
- ^ "Barboza Named MD at Hot 96.9". Greater Media. February 20, 2013. Retrieved March 2, 2013.
- ^ "Baltazar Returns to Boston to Co-Host Mornings on Hot 96.9 FM". Greater Media. March 1, 2013. Retrieved March 2, 2013.
- ^ http://www.bostonradio.org/stations/25050
External links[edit]
- Official website
- Station History
- Query the FCC's FM station database for WBQT
- Radio-Locator information on WBQT
- Query Nielsen Audio's FM station database for WBQT


text: We like us some matte finish here at Autoblog, but we have to admit that the usual smattering of matte black vinyl wraps leave us a bit in the dark. That's why our eyes pop just a little wider when we see a car that cuts the glare but keeps the palette. That's also why the storied brands of the Fiat group are getting top marks in our books these days.


text:
The Year in Television, 2009: A Catalog of New and Continuing Series, Miniseries, Specials and TV Movies
McFarland, Sep 3, 2010 - Performing Arts - 315 pages
This reference work is a chronicle of all the first run entertainment programs broadcast from January 1 to December 31, 2009. Included are series, TV movies, aired pilots, specials, miniseries and Internet series. Alphabetically arranged entries provide casts, storylines, production credits, networks, broadcast dates, and excerpts from newspaper reviews. New to this volume is a listing of the highlights of the year and coverage of all the unaired pilots produced for the 2008-2009 season.
What people are saying - Write a review
We haven't found any reviews in the usual places.
Selected pages
Returning SeriesDebuted Prior to 2009
Other editions - View all
The Year in Television, 2008: A Catalog of New and Continuing Series ...
Vincent Terrace
No preview available - 2009
The Year in Television, 2009: A Catalog of New and Continuing Series ...
Vincent Terrace
No preview available - 2010
Common terms and phrases
ABC Family Adam Alex Andy April Awards Barack Obama BBC America become Bill Billy Brian broadcast Cartoon Cartoon Network celebrity Charlie Chris Comedy Comedy Central contestants Credits Crime Drama Dane Cook Daniel Danny David Director ducer Emily episode Eric Executive Producer film friends Game girl Guests Hollywood Reporter Host Jack James Jane Jason Jeff Jennifer Jessica John July June Kate Katie Kelly Kevin Kyle Larry Lisa lives married Mary Matt Michael miered Mike Miley Cyrus mother Music N.Y. Daily N.Y. Post Narrator Network Nick Paul perform Peter player Premiered Reality Reviews Richard Robert Ryan Samantha Sarah Scott Sept Series Special Spike TV Star Steve Stories follow Supervising Producer Taylor Taylor Swift teenage television Tony TV Movie Variety viewers Voice Cast Wanda Sykes Williams wins women Writer young


text:
Connective tissue growth factor (CCN2) is a matricellular protein that plays a role in hepatic stellate cell (HSC)-mediated fibrogenesis. The aim of this study was to investigate the regulation by CCN2 of cell survival pathways in primary HSC.
Primary HSC were obtained by in situ enzymatic perfusion of rat liver. NF-κB activation was assessed by immunoblotting for IκBα phosphorylation and degradation and by NF-κB p50 or p65 nuclear accumulation. NF-κB DNA-binding activity was determined by gel mobility shift assay while NF-κB response gene expression was evaluated using a luciferase reporter. Cell viability was assessed by Trypan blue staining or ATP luminescent assay while apoptosis was evaluated by caspase-3 activity.
CCN2 induced IκBα phosphorylation and degradation as well as nuclear accumulation of NF-κB. Activated NF-κB comprised three dimers, p65/p65, p65/p50 and p50/p50, that individually bound to DNA-binding sites and subsequently triggered transcriptional activity. This was confirmed by showing that CCN2 promoted activity of a NF-κB luciferase reporter. CCN2 promoted survival of serum-starved HSC and protected the cells from death induced by blocking the NF-κB signaling pathway using Bay-11-7082, a specific inhibitor of IκBα phosphorylation.
CCN2 contributes to the survival of primary HSC through the NF-κB pathway.
Keywords:connective tissue growth factor; CCN2; hepatic stellate cell; NF-κB; survival; apoptosis; fibrosis
Hepatic stellate cells (HSC) are the primary targets of fibrogenic stimuli in the injured liver. During the development of fibrosis, HSC undergo a transition from resting vitamin A-rich cells to an activated myofibroblastic phenotype characterized by loss of vitamin A, expression of α-smooth muscle actin, enhanced proliferation and increased production of various extracellular matrix components [1-4]. Activation of HSC has been identified as a central event in hepatic fibrosis and is regulated by a wide variety of molecules including cytokines, cell-surface receptors, signal transduction molecules and factors that regulate HSC gene expression at the transcriptional and post-transcriptional levels [3-6].
Connective tissue growth factor (CCN2, also known as CTGF) is a cysteine-rich matricellular protein that regulates cell adhesion, migration, proliferation, survival, and differentiation [7]. It has fibrogenic properties in vitro and is over-expressed in many fibrotic lesions, including those of the skin, lung, kidney and liver [8-12]. CCN2 production is enhanced during progressive activation of primary rat HSC in vitro as well as by transforming growth factor-β [11-13]. CCN2 induces migration, proliferation and adhesion of HSC as well as enhanced expression of type I collagen [14-19].
Transcription factor NF-κB is a key regulator of the growth, differentiation, and fate of mammalian cells [20]. NF-κB exists in virtually all cell types and represents a family of inducible transcription factors that are activated by a variety of stimuli including viral infection, lipopolysaccharide, oxidative stress, and cytokines [21]. The active form of NF-κB is found in the nucleus as either a heterodimer or a homodimer composed of five members of the Rel family of proteins (p65, p50, p52, c-Rel, and RelB) [20]. It has recently been reported that transcriptional repressor CBF1 plays a key role in regulating NF-κB activity through its interaction with a dual NF-κB/CBF1-binding site in the IκBα promoter [22,23]. IκBα regulates NF-κB activity by directly interacting with the transcriptional factor to form inactive complexes that are located to the cytoplasm. Following specific signaling, phosphorylation of IκBα at serine 32 and 36 by IκB kinase leads to its ubiquitinylation and degradation by the proteasome, and transport of active NF-κB to nucleus [24]. Active NF-κB is involved in the expression of numerous cytokines, acute phase response proteins, adhesion molecules and Rel/IκB proteins [20]. Also, when NF-κB activation is prevented or inhibited, cells undergo enhanced apoptosis showing that active NF-κB exerts a cytoprotective role by inhibiting apoptosis [25].
Several studies have compared NF-κB activity in quiescent versus activated HSC [26-28]. NF-κB activity is increased in cultured activated HSC but it is not required for either cell proliferation or the process of activation. In contrast, active NF-κB plays an important role in preventing apoptosis of activated HSC [26,29]. Understanding mechanisms of HSC survival may provide the basis for novel anti-fibrotic therapies that focus on the ability to clear activated HSC from the liver by inducing them to undergo apoptosis. Since the principal CCN2 receptor on HSC is integrin αvβ3 [19], which is intimately associated with HSC survival [30], we have investigated the role of CCN2 in NF-κB activation and HSC survival.
CCN2 induces phosphorylation of IκBα and translocation of NF-κB
In most cell types, NF-κB is found in the cytoplasm as an inactive dimer bound to one of the IκB inhibitory proteins (IκBα, IκBβ, or IκBγ) that mask its nuclear localization signal. As assessed by Western blotting of cytoplasmic protein extracts day 4 primary HSC, phospho-IκBα was elevated while total IκBα was decreased following stimulation by CCN2 (Figure 1A), indicating that CCN2 could induce IκBα phosphorylation and degradation. Additionally, following CCN2 stimulation, levels of p65 and p50 were reduced in the cytoplasm but increased in the nucleus (Figure 1B), consistent with the notion that CCN2-induced IκBα phosphorylation and degradation was associated with translocation of cytoplasmic NF-κB to the nucleus.
Figure 1. Effect of CCN2 on stimulation of IκBα phophorylation and NF-κB translocation. Freshly isolated rat HSC were cultured for 24 h in 5% FBS DMEM and for another 48 h in serum-free medium. The cells were harvested following incubating the cells for 30 min in the absence or presence of 100 ng/ml CCN2, and nuclear extracts were prepared. Western blot analysis shows that CCN2 induces IκB phosphorylation and degradation (A) and translocation of the NF-κB subunits p65/p50 from cytoplasm to nucleus (B).
CCN2 promotes NF-κB DNA binding activity
To further explore whether active NF-κB can bind to its target DNA sequence and activate gene transcription in response to CCN2 stimulation, NF-κB DNA binding activity was determined by EMSA following incubation of HSC nuclear protein extracts with 32P-labeled NF-κB oligomers containing NF-κB/CBF1 binding sites. Two complexes were significantly enhanced by CCN2, reaching a plateau about 30 min after CCN2 addition (Fig. 2A). The NF-κB inhibitor, Bay11-7082, prevented complex formation when added to the cells prior to CCN2 treatment but not when added after CCN2 treatment (Figure 2B). As shown in Figure 2C, active NF-κB induced by CCN2 comprised three separate dimers (p65/p65, p65/p50 and p50/p50) based on the fact that a supershift (S1) was obtained with anti-p65 and anti-p50 antibodies with the concomitant disappearance of all three bands and that a supershift (S2) with anti-p65 antibody was associated with loss of the top two bands. Three dimers of NF-κB induced by CCN2 were also demonstrated following incubation of the nuclear extract with a CBF-1 mutant oligonucleotide probe but not with a p50/p65 mutant oligonucleotide probe (Figure 2D). These results indicate that CCN2 induces activation of NF-κB and its assembly into three dimers that individually bind to NF-κB DNA binding site.
Figure 2. Modulation of NF-κB DNA binding activity by CCN2. HSC were harvested at the desired time points after treatment with or without 100 ng/ml CCN2. 6 μg nuclear protein extract were used in 20 μl reactions, containing 0.2 ng 32P-labeled double strand NF-κB oligonucleotides. Reactions were fractioned through a nondenaturing 4% polyacrylamide gel. (A) Complex 1 (p65/p50) and complex 2 were enhanced after stimulation with CCN2. "Ctrl" represents a reaction lacking nuclear extract. (B) Bay11-7082 inhibited complex formation when added prior to CCN2 treatment ("Bay 11 + CCN2") but not when added subsequent to a 1 hour pretreatment with CCN2 ("CCN2 + Bay11"). (C) A supershift assay was performed by incubating pre-assembled gel shift assay complexes containing 8 μg nuclear extract with either 2 μg normal rabbit IgG or 2 μg anti-NF-κB antibody prior to separation through 8% polyacrylamide gel, showing that CCN2 stimulates the formation of an anti-p65/p65/anti-p50/p50/NF-κB oligonucleotide (S1) and an anti-p65/p65/NF-κB oligonucleotide (S2) supershift complexes. (D) A gel shift assay was performed following pre-incubating the nuclear extracts with either 32P-labeled p50/p65 site mutant oligonucleotides or CBF-1 site mutant oligonucleotides.
Effect of CCN2 on expression of NF-κB target genes
To explore the effect of CCN2 on transcriptional signaling by NF-κB, day 4 primary HSC cultures were transfected with luciferase reporter genes driven by either a minimal promoter alone (pTA) or together with four tandem copies of the NF-κB DNA binding sites (pNF-κB) that were identical to those used for the EMSA studies. pNF-κB generated 1.5-fold higher luciferase activities than pTA in HSC, whereas, the luciferase activities of pNF-κB in the cells following stimulation with CCN2 were 13-fold higher than control cells (Figure 3A). CCN2-mediated elevation of pNF-κB luciferase activity was completely abrogated by treatment of the cells with Bay11-7082, a specific inhibitor of IκBα phosphorylation (Figure 3B), suggesting that CCN2 modulates the transcriptional and translational event of NF-κB target genes via NF-κB signaling pathway.
Figure 3. Effect of CCN2 on expression of NF-κB response genes. (A) Freshly isolated rat HSC were placed in 12-well plates, transfected with 1 μg pTA-Luc or pNF-κB-Luc, and then incubated for another 24 h in the absence or presence of 100 ng/ml CCN2. (B) Transfected cells were pre-treated with 10 μM Bay11-7082 for 30 min and cultured for another 24 h in the absence or presence of 100 ng/ml CCN2. **P < 0.01 vs. pNF-κB-Luc; ##P < 0.01 vs. pNF-κB-Luc + CCN2.
CCN2 sustains HSC survival through NF-κB signaling pathway
To examine the effect of CCN2 on the fate of HSC, day 4-primary HSC cultures were treated with or without CCN2 for 24 h. Cell viability was determined by Trypan blue exclusion or by luminescent assessment of cellular ATP levels. As shown in Figures 4A and 4B, each assay showed that HSC viability was significantly elevated by CCN2. To determine if the NF-κB pathway was involved in this effect, Bay11-7082 was added to HSC cultures. As mentioned above, when added prior to CCN2, the inhibitor completely blocked the ability of CCN2 to stimulate NF-κB DNA binding activity whereas it had little inhibitory effect on DNA binding activity in cells that has been pre-treated with CCN2 (Figure 2B). While Bay11-7082 inhibited cell survival as expected, it had little inhibitory effect in cells that had been pre-treated with CCN2 (Figure 4A,B), consistent with the data shown in Figure 2B and supportive of the notion that prior stimulation of NF-κB by CCN2 was sufficient to overcome the effects of subsequently blocking NF-κB with Bay 11. Similarly, Bay-11-induced caspase-3 activity in HSC was reduced as much as 25% in CCN2-stimulated cells, consistent with the ability of CCN2 to rescue cells from apoptosis (Figure 4C).
Figure 4. Effect of CCN2 in sustaining HSC survival. Freshly isolated rat HSC were cultured in 6-well plates in 5% FBS DMEM for 24 h, followed by serum deprivation for 48 h. The cells were cultured for another 24 h in the absence or presence of 100 ng/ml CCN2 ("CCN2"). In the CCN2 protection assay, the cells were incubated with 10 μM Bay11-7082 for 24 h alone ("Bay11") or following pre-treatment of the cells with CCN2 for 1 h ("CCN2+Bay11"). For the Bay11 blocking assay, the cells were pre-treated with Bay11-7082 for 30 min and cultured for another 24 h in the presence of 100 ng/ml CCN2 ("Bay11+CCN2"). At the end of incubation time period, (A) cells were trypsinized and survival was determined by Trypan blue exclusion, or (B) cell viability was also quantified by measurement of the fluorescence intensity using CellTiter-Glo™ reagent, or (C) cell apoptosis was assessed by measurement of caspase-3 activity at 405 nm using a luminescence assay kit. *P < 0.05 vs. control; #P < 0.05 vs. CCN2 group; **P < 0.01 and * P < 0.05 vs. Bay11-7082 group.
Collectively, these data suggest that CCN2 is a survival factor for HSC and that survival is regulated via NF-κB signaling.
CCN2 has emerged as a key mediator of fibrosis in both acute and chronic diseases [8-12]. In the liver, CCN2 expression is associated with hepatic fibrosis in both human subjects and animal models [10-12,14,17,31]. CCN2 appears to be directly involved in HSC biology as it is produced as a function of activation or exposure the cells to various fibrogenic stimuli including transforming growth factor-β, platelet-derived growth factor, alcohol and acetaldehyde [14]. Additionally, CCN2 promotes HSC adhesion, migration, proliferation, and synthesis of collagen type I [14,19], all of which are properties of activated HSC. [1-4,32]. Activation of HSC is regulated by several soluble factors, including growth factors, cytokines, and products of oxidative stress, as well as by extensive changes in the composition and organization of the extracellular matrix. HSC activation has previously been linked to activation of NF-κB while over-expression of IκBα in HSC has been shown to suppress NF-κB activation [26-29]. Our data show that serum-starved day 4 HSC demonstrate very low levels of nuclear NF-κB and no detectable DNA-binding activity. Following CCN2 stimulation, a marked nuclear translocation of NF-κB was evident along with a persistent DNA binding activity of its three dimers, and an induction of IκBα phosphorylation and degradation. Collectively, these data show that CCN2 activates the NF-κB signaling pathway in HSC. Moreover, as assessed using a NF-κB reporter construct, we showed that NF-κB response gene expression is induced by CCN2 in day 4 cultured HSC and support previous findings that NF-κB response genes are induced in activated HSC [26,29].
Apoptosis has been described as the nexus between liver injury and fibrosis [33] and increasing evidence suggests that NF-κB is involved in survival pathways in multiple hepatic cell types. For example, NF-κB prevents hepatocyte apoptosis following liver regeneration or exposure to tumor necrosis factor-α [34-36]. In addition, NF-κB also protects hepatocarcinoma cells or activated HSC from apoptosis [26,29,37]. Consistent with this latter observation, we showed that CCN2 promoted HSC survival and that NF-κB was involved in the response as shown by the finding that pretreatment of HSC with CCN2 protected the cells from Bay11-7082-induced decreased cell survival and increased caspase-3 activity.
The ability of CCN2 to sustain HSC survival supports previous observations showing that CCN2 is a survival factor for other cell types such as endothelial cells or chicken embryo fibroblasts [38,39]. Additionally, the related molecule, CCN1 (also known as CYR61) promotes anti-apoptotic pathways when over-expressed in breast cancer MCF7 cells in an integrin-dependent manner [40], consistent with the recognition of integrins as signaling receptors for CCN proteins [41]. In the case of HSC, the principal CCN2 receptor is integrin αvβ3 [19] which transduces survival signals in activated HSC [30] as well as during angiogenesis, wound healing, osteoporosis, and tumor metastasis [42-51]. Apoptosis in HSC is inhibited by engagement of integrin αvβ3 [30] and disruption of integrin-mediated HSC adhesion leads to induction of apoptosis [52]. Since expression of CCN2 and integrins is enhanced during HSC activation and liver fibrosis [53-57], persistence of the activated fibrogenic phenotype in HSC may occur, at least in part, by NF-κB survival pathways that are triggered via the binding of CCN2 to its integrin αvβ3 receptor.
Activation of HSC is also associated with the expression of death receptors such as Fas and TRAIL-R2, suggesting that HSC fate is likely determined by a balance between survival and apoptotic stimuli [33]. For example, HSC undergo apoptosis following treatment with nerve growth factor, a response that is due to the expression of the p75 nerve growth factor receptor [58] which has recently been implicated as a CCN2 signaling molecule in kidney mesangial cells [59]. Furthermore, pro-apoptotic effects of CCN2 have been reported in vascular smooth muscle cells and breast cancer cells, although the underlying mechanisms have yet to be understood [60-62]. Thus, depending on the presence and activity of its cognate cell surface receptors and their associated signaling pathways, CCN2 may be able to drive either apoptosis or survival in HSC. This points to a complex scenario whereby CCN2 may exert apparently opposing or contradictory effects on HSC viability, and future investigations will need to clarify this issue. Nonetheless, clinical fibrosis is now regarded as a largely reversible process that is strongly linked to apoptosis of activated HSC [33,63] and our data showing that CCN2 can promote HSC survival via NF-κB provide support for the development of new anti-fibrotic strategies that target CCN2, its receptors, or its signaling pathways.
In addition to promoting HSC fibrogenesis [17], CCN2 confers a survival advantage on HSC which is attributable, at least in part, to its ability to activate NF-κB signaling pathways in the cells.
Isolation and culture of HSC
In a protocol approved by the Institutional Animal Care and Use Committee of Children's Research Institute, Columbus, OH, primary HSC were isolated from normal male Sprague-Dawley rats as described [19]. Cells were grown in Dulbecco's modified Eagle's medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS), 100 U/ml penicillin and 100 μg/ml streptomycin. Cells were placed in 20 × 100 mm cell culture dishes (Falcon; Becton Dickinson, Franklin Lakes, NJ, USA) for nuclear extraction, 6-well tissue culture plates (Falcon) for Western blot and for cell viability assays, 12-well tissue culture plates (Falcon) for luciferase reporter gene transfection. The cells were then cultured DMEM/5% FBS for 24 h, followed by serum-free medium for 48 h. On day 4, the cells were treated with or without 100 ng/ml CCN2, and harvested at the desired time points. Human recombinant 38 kDa CCN2 was produced in a Chinese hamster ovary cell expression system as described [15].
Electrophoretic mobility shift assay (EMSA)
Nuclear extracts were prepared as described [64]. 32P-end-labeled double-stranded oligonucleotide probes used in this study comprised either wild type NF-κB oligonucleotide (sense: 5'-tgaggggactttcccagg-3'), p50/p65 mutant oligonucleotide (sense: 5'-tgaggcgactttcccagg-3') or CBF1-mutant oligonucleotide (sense: 5'-tgaggggacttcccgagg-3') [23]. The double-stranded NF-κB oligmers were used in nuclear protein-DNA binding reactions (20 μl volume) in which 1 μg poly dI:dC and 6 μg nuclear protein extract were incubated for 20 min at 4°C prior to addition of 0.2 ng 32P-labled double-stranded oligonucleotide for 30 min at 4°C. The contents of each tube were electrophoresed on non-denaturing 4% polyacrylamide gels which were then dried and analyzed by autoradiography. Supershift assays were performed by incubating pre-assembled gel shift assay complexes containing 8 μg nuclear extract with either 2 μg rabbit normal IgG, 2 μg rabbit polyclonal anti- p65 NF-κB IgG or/and 2 μg rabbit polyclonal anti- p50 NF-κB IgG (Santa Cruz Biotechnology Inc, CA, USA) for 2 h at 4°C before electrophoresis. The samples were then electrophoresed on 8 % polyacrylamide gels [65].
Transfections and luciferase assay
HSC were transfected with pNF-κB-Luc or pTA-Luc control vector using Superfect transfection reagent (QIAGEN, Valencia, CA, USA) under serum-free conditions for 3 h. The transfected cells were incubated for another 24 h in the absence or presence of 100 ng/ml CCN2. After normalization of transfection efficiency by β-galactosidase expression, luciferase enzyme activity was then quantified using a reporter assay kit (Clontech, Palo Alto, CA, USA).
SDS-PAGE and immunoblotting
25 μg cytoplasmic or nuclear extracts (see above) were subjected to SDS-PAGE in 5–15% gradient gels at 120 V for 1.5 h. Proteins were transferred to nitrocellulose membranes which were individually incubated with 1:500 dilutions of rabbit anti-IκBα, -phospho-IκBα, -NF-κB p65, or -NF-κB p50 polyclonal IgG (Santa Cruz Biotechnology Inc, CA, USA) in 5% nonfat milk TBST for 24 h at 4°C. The filters were then incubated with 1:1000 dilutions of HRP-conjugated goat anti-rabbit IgG for 1 h at room temperature. The membrane was washed extensively before detection using chemiluminescence.
Cell viability assay
Freshly isolated HSC were placed in 6-well tissue culture plates and incubated in DMEM/5% FBS for 24 h followed by serum-free medium for 48 h. The cells were incubated for an additional 24 h in the absence or presence of 100 ng/ml CCN2. In CCN2 protection assays, 10 μM Bay11-7082 was added to the medium either alone or following treatment of the cells with CCN2 for 1 h. At the end of the incubation period, the cells were trypsinized, mixed 1:1 with Trypan Blue solution (Sigma) and counted within 3 minutes under light microscopy using a hemocytometer.
In an alternative approach for measurement of cell viability, the CellTiter-Glo™ Luminescent assay kit was employed to assess the relative levels of cellular ATP. At the end of the incubation period, cells were treated with CellTiter-Glo™ reagent according to the manufacturer's instructions. Fluorescence was measured using black/clear tissue culture plates (BD Biosciences, Bedford, MA, USA). Cell viability was quantified by measurement of the sample fluorescence intensity at 560EX/590EM.
Caspase-3 activity assay
Freshly isolated HSC were placed in 12-well tissue culture plate, and incubated in DMEM/5% FBS for 24 h followed by serum-free medium for 48 h. The cells were incubated for an additional 24 h in the presence or absence of 100 ng/ml CCN2. 10 μM Bay11-7082 was added to the medium either alone or following treatment the cells with CCN2 for 1 h. Protein extracts were prepared following manufacturer's instructions. Caspase-3 activity was measured using an assay kit (Promega, Madison, WI, USA) in which cell extracts were mixed with Ac-DEVD-pNA substrate for 1-hour incubation at 30°C in 96-well microtiter plates prior to colorimetric measurement of p-nitroanilide product at 405 nm.
Statistical analysis
Data are presented as mean ± SE. Differences were analyzed statistically with paired sample student's t-test.
Competing interests
The author(s) declare that they have no competing interests.
Authors' contributions
RG carried out the experiments and wrote the draft manuscript. DRB participated in the experimental design and edited the manuscript.
This work was supported by NIH grant AA12817 awarded to D.R.B.
Gressner AM, Bachem MG: Cellular communications and cell-matrix interactions in the pathogenesis of fibroproliferative diseases: liver fibrosis as a paradigm.
Pinzani M: Novel insights into the biology and physiology of the Ito cell.
Pharmacol Ther 1995, 66(2):387-412. Publisher Full Text
Eng FJ, Friedman SL: Fibrogenesis I. New insights into hepatic stellate cell activation: the simple becomes complex.
Am J Physiol Gastrointest Liver Physiol 2000, 279(1):G7-G11.
Friedman SL: Molecular regulation of hepatic fibrosis, an integrated cellular response to tissue injury.
J Biol Chem 2000, 275(4):2247-2250. Publisher Full Text
Pinzani M, Gesualdo L, Sabbah GM, Abboud HE: Effects of platelet-derived growth factor and other polypeptide mitogens on DNA synthesis and growth of cultured rat liver fat-storing cells.
Friedman SL: Cytokines and fibrogenesis.
Brigstock DR: The connective tissue growth factor/cysteine-rich 61/nephroblastoma overexpressed (CCN) family.
Endocr Rev 1999, 20(2):189-206. Publisher Full Text
Igarashi A, Nashiro K, Kikuchi K, Sato S, Ihn H, Fujimoto M, Grotendorst GR, Takehara K: Connective tissue growth factor gene expression in tissue sections from localized scleroderma, keloid, and other fibrotic skin disorders.
J Invest Dermatol 1996, 106(4):729-733. Publisher Full Text
Lasky JA, Ortiz LA, Tonthat B, Hoyle GW, Corti M, Athas G, Lungarella G, Brody A, Friedman M: Connective tissue growth factor mRNA expression is upregulated in bleomycin-induced lung fibrosis.
Abou-Shady M, Friess H, Zimmermann A, di Mola FF, Guo XZ, Baer HU, Buchler MW: Connective tissue growth factor in human liver cirrhosis.
Liver 2000, 20(4):296-304. Publisher Full Text
Williams EJ, Gaca MD, Brigstock DR, Arthur MJ, Benyon RC: Increased expression of connective tissue growth factor in fibrotic human liver and in activated hepatic stellate cells.
J Hepatol 2000, 32(5):754-761. Publisher Full Text
Paradis V, Dargere D, Vidaud M, De Gouville AC, Huet S, Martinez V, Gauthier JM, Ba N, Sobesky R, Ratziu V, Bedossa P: Expression of connective tissue growth factor in experimental rat and human liver fibrosis.
Hepatology 1999, 30(4):968-976. Publisher Full Text
Gao R, Brigstock DR: Low density lipoprotein receptor-related protein (LRP) is a heparin-dependent adhesion receptor for connective tissue growth factor (CTGF) in rat activated hepatic stellate cells.
Hepatol Res 2003, 27(3):214-220. Publisher Full Text
Paradis V, Dargere D, Bonvoust F, Vidaud M, Segarini P, Bedossa P: Effects and regulation of connective tissue growth factor on hepatic stellate cells.
Ball DK, Moussad EE, Rageh MA, Kemper SA, Brigstock DR: Establishment of a recombinant expression system for connective tissue growth factor (CTGF) that models CTGF processing in utero.
Reproduction 2003, 125(2):271-284. Publisher Full Text
Ball DK, Rachfal AW, Kemper SA, Brigstock DR: The heparin-binding 10 kDa fragment of connective tissue growth factor (CTGF) containing module 4 alone stimulates cell adhesion.
J Endocrinol 2003, 176(2):R1-7. Publisher Full Text
Rachfal AW, Brigstock DR: Connective tissue growth factor (CTGF/CCN2) in hepatic fibrosis.
Hepatol Res 2003, 26(1):1-9. Publisher Full Text
Gao R, Ball DK, Perbal B, Brigstock DR: Connective tissue growth factor induces c-fos gene activation and cell proliferation through p44/42 MAP kinase in primary rat hepatic stellate cells.
J Hepatol 2004, 40(3):431-438. Publisher Full Text
Gao R, Brigstock DR: Connective tissue growth factor (CCN2) induces adhesion of rat activated hepatic stellate cells by binding of its C-terminal domain to integrin {alpha}v{beta}3 and heparan sulfate proteoglycan.
J Biol Chem 2004, 279(10):8848-8855. Publisher Full Text
Ghosh S, May MJ, Kopp EB: NF-kappa B and Rel proteins: evolutionarily conserved mediators of immune responses.
Annu Rev Immunol 1998, 16:225-260. Publisher Full Text
Ghosh S, Karin M: Missing pieces in the NF-kappaB puzzle.
Cell 2002, 109 Suppl:S81-96. Publisher Full Text
Berti R, Williams AJ, Moffett JR, Hale SL, Velarde LC, Elliott PJ, Yao C, Dave JR, Tortella FC: Quantitative real-time RT-PCR analysis of inflammatory gene expression associated with ischemia-reperfusion brain injury.
J Cereb Blood Flow Metab 2002, 22(9):1068-1079. Publisher Full Text
Oakley F, Mann J, Ruddell RG, Pickford J, Weinmaster G, Mann DA: Basal expression of IkappaBalpha is controlled by the mammalian transcriptional repressor RBP-J (CBF1) and its activator Notch1.
J Biol Chem 2003, 278(27):24359-24370. Publisher Full Text
Mann DA, Smart DE: Transcriptional regulation of hepatic stellate cell activation.
Gut 2002, 50(6):891-896. Publisher Full Text
Sonenshein GE: Rel/NF-kappa B transcription factors and the control of apoptosis.
Semin Cancer Biol 1997, 8(2):113-119. Publisher Full Text
Elsharkawy AM, Wright MC, Hay RT, Arthur MJ, Hughes T, Bahr MJ, Degitz K, Mann DA: Persistent activation of nuclear factor-kappaB in cultured rat hepatic stellate cells involves the induction of potentially novel Rel-like factors and prolonged changes in the expression of IkappaB family proteins.
Hepatology 1999, 30(3):761-769. Publisher Full Text
Hellerbrand C, Jobin C, Licato LL, Sartor RB, Brenner DA: Cytokines induce NF-kappaB in activated but not in quiescent rat hepatic stellate cells.
Rippe RA, Schrum LW, Stefanovic B, Solis-Herruzo JA, Brenner DA: NF-kappaB inhibits expression of the alpha1(I) collagen gene.
DNA Cell Biol 1999, 18(10):751-761. Publisher Full Text
Lang A, Schoonhoven R, Tuvia S, Brenner DA, Rippe RA: Nuclear factor kappaB in proliferation, activation, and apoptosis in rat hepatic stellate cells.
J Hepatol 2000, 33(1):49-58. Publisher Full Text
Zhou X, Murphy FR, Gehdu N, Zhang J, Iredale JP, Benyon RC: Engagement of alpha Vbeta 3 integrin regulates proliferation and apoptosis of hepatic stellate cells.
Hayashi N, Kakimuma T, Soma Y, Grotendorst GR, Tamaki K, Harada M, Igarashi A: Connective tissue growth factor is directly related to liver fibrosis.
Gaca MD, Zhou X, Benyon RC: Regulation of hepatic stellate cell proliferation and collagen synthesis by proteinase-activated receptors.
J Hepatol 2002, 36(3):362-369. Publisher Full Text
Canbay A, Friedman S, Gores GJ: Apoptosis: the nexus of liver injury and fibrosis.
Hepatology 2004, 39(2):273-278. Publisher Full Text
Iimuro Y, Nishiura T, Hellerbrand C, Behrns KE, Schoonhoven R, Grisham JW, Brenner DA: NFkappaB prevents apoptosis and liver dysfunction during liver regeneration.
Black D, Bird MA, Hayden M, Schrum LW, Lange P, Samson C, Hatano E, Rippe RA, Brenner DA, Behrns KE: TNF alpha-induced hepatocyte apoptosis is associated with alterations of the cell cycle and decreased stem loop binding protein.
Surgery 2004, 135(6):619-628. Publisher Full Text
Imose M, Nagaki M, Naiki T, Osawa Y, Brenner DA, Asano T, Hayashi H, Kato T, Moriwaki H: Inhibition of nuclear factor kappaB and phosphatidylinositol 3-kinase/Akt is essential for massive hepatocyte apoptosis induced by tumor necrosis factor alpha in mice.
Liver Int 2003, 23(5):386-396. Publisher Full Text
Chiao PJ, Na R, Niu J, Sclabas GM, Dong Q, Curley SA: Role of Rel/NF-kappaB transcription factors in apoptosis of human hepatocellular carcinoma cells.
Cancer 2002, 95(8):1696-1705. Publisher Full Text
Babic AM, Chen CC, Lau LF: Fisp12/mouse connective tissue growth factor mediates endothelial cell adhesion and migration through integrin alphavbeta3, promotes endothelial cell survival, and induces angiogenesis in vivo.
Gygi D, Zumstein P, Grossenbacher D, Altwegg L, Luscher TF, Gehring H: Human connective tissue growth factor expressed in Escherichia coli is a non-mitogenic inhibitor of apoptosis.
Biochem Biophys Res Commun 2003, 311(3):685-690. Publisher Full Text
Lin MT, Chang CC, Chen ST, Chang HL, Su JL, Chau YP, Kuo ML: Cyr61 expression confers resistance to apoptosis in breast cancer MCF-7 cells by a mechanism of NF-kappaB-dependent XIAP up-regulation.
J Biol Chem 2004, 279(23):24015-24023. Publisher Full Text
Lau LF, Lam SC: The CCN family of angiogenic regulators: the integrin connection.
Exp Cell Res 1999, 248(1):44-57. Publisher Full Text
Cheresh DA, Spiro RC: Biosynthetic and functional properties of an Arg-Gly-Asp-directed receptor involved in human melanoma cell attachment to vitronectin, fibrinogen, and von Willebrand factor.
Cheresh DA, Pytela R, Pierschbacher MD, Klier FG, Ruoslahti E, Reisfeld RA: An Arg-Gly-Asp-directed receptor on the surface of human melanoma cells exists in an divalent cation-dependent functional complex with the disialoganglioside GD2.
J Cell Biol 1987, 105(3):1163-1173. Publisher Full Text
Gladson CL, Cheresh DA: Glioblastoma expression of vitronectin and the alpha v beta 3 integrin. Adhesion mechanism for transformed glial cells.
Schwartz MA: Signaling by integrins: implications for tumorigenesis.
Brooks PC, Clark RA, Cheresh DA: Requirement of vascular integrin alpha v beta 3 for angiogenesis.
Brooks PC, Montgomery AM, Rosenfeld M, Reisfeld RA, Hu T, Klier G, Cheresh DA: Integrin alpha v beta 3 antagonists promote tumor regression by inducing apoptosis of angiogenic blood vessels.
Cell 1994, 79(7):1157-1164. Publisher Full Text
Montgomery AM, Reisfeld RA, Cheresh DA: Integrin alpha v beta 3 rescues melanoma cells from apoptosis in three-dimensional dermal collagen.
Drake CJ, Cheresh DA, Little CD: An antagonist of integrin alpha v beta 3 prevents maturation of blood vessels during embryonic neovascularization.
Scatena M, Almeida M, Chaisson ML, Fausto N, Nicosia RF, Giachelli CM: NF-kappaB mediates alphavbeta3 integrin-induced endothelial cell survival.
J Cell Biol 1998, 141(4):1083-1093. Publisher Full Text
Miyauchi A, Alvarez J, Greenfield EM, Teti A, Grano M, Colucci S, Zambonin-Zallone A, Ross FP, Teitelbaum SL, Cheresh D, et al.: Recognition of osteopontin and related peptides by an alpha v beta 3 integrin stimulates immediate cell signals in osteoclasts.
Iwamoto H, Sakai H, Tada S, Nakamuta M, Nawata H: Induction of apoptosis in rat hepatic stellate cells by disruption of integrin-mediated cell adhesion.
J Lab Clin Med 1999, 134(1):83-89. Publisher Full Text
Reeves HLDCLDCPBAD: ß1-integrin expression in hepatic stellate cells from normal and diseased human livers: 1997; Bordeaux, France,. Volume 6. Edited by Wisse EKDLBC. The Kupffer Cell Foundation, Leiden, The Netherlands; 1997::154-155.
Richter HB, Franke H, Dargel R: Expression of tenascin, fibronectin, and laminin in rat liver fibrogenesis--a comparative immunohistochemical study with two models of liver injury.
Carloni V, Romanelli RG, Pinzani M, Laffi G, Gentilini P: Expression and function of integrin receptors for collagen and laminin in cultured human hepatic stellate cells.
Gastroenterology 1996, 110(4):1127-1136. Publisher Full Text
Iwamoto H, Sakai H, Nawata H: Inhibition of integrin signaling with Arg-Gly-Asp motifs in rat hepatic stellate cells.
J Hepatol 1998, 29(5):752-759. Publisher Full Text
Kato R, Kamiya S, Ueki M, Yajima H, Ishii T, Nakamura H, Katayama T, Fukai F: The fibronectin-derived antiadhesive peptides suppress the myofibroblastic conversion of rat hepatic stellate cells.
Exp Cell Res 2001, 265(1):54-63. Publisher Full Text
Trim N, Morgan S, Evans M, Issa R, Fine D, Afford S, Wilkins B, Iredale J: Hepatic stellate cells express the low affinity nerve growth factor receptor p75 and undergo apoptosis in response to nerve growth factor stimulation.
Wahab NA, Weston BS, Mason RM: Connective tissue growth factor CCN2 interacts with and activates the tyrosine kinase receptor TrkA.
J Am Soc Nephrol 2005, 16(2):340-351. Publisher Full Text
Hishikawa K, Nakaki T, Fujii T: Connective tissue growth factor induces apoptosis via caspase 3 in cultured human aortic smooth muscle cells.
Eur J Pharmacol 2000, 392(1-2):19-22. Publisher Full Text
Hishikawa K, Oemar BS, Tanner FC, Nakaki T, Fujii T, Luscher TF: Overexpression of connective tissue growth factor gene induces apoptosis in human aortic smooth muscle cells.
Hishikawa K, Oemar BS, Tanner FC, Nakaki T, Luscher TF, Fujii T: Connective tissue growth factor induces apoptosis in human breast cancer cell line MCF-7.
J Biol Chem 1999, 274(52):37461-37466. Publisher Full Text
Friedman SL, Arthur MJ: Reversing Hepatic Fibrosis.
Dignam JD, Lebovitz RM, Roeder RG: Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
Pizzi M, Goffi F, Boroni F, Benarese M, Perkins SE, Liou HC, Spano P: Opposing roles for NF-kappa B/Rel factors p65 and c-Rel in the modulation of neuron survival elicited by glutamate and interleukin-1beta.
J Biol Chem 2002, 277(23):20717-20723. Publisher Full Text


text:
realmomsrealviews.com Real Moms Real Views – mommy blog product reviews giveaways
mommy blog product reviews giveaways
circleofmoms.com Circle of Moms - Online mom groups, mom blogs, and parenting resources around motherhood, new...
Circle of Moms is the largest and fastest-growing online community for moms, connecting over 6 million moms worldwide to share advice and support...
indianapolis.momslikeme.com The Indianapolis Star, Indystar
Indianapolis Indiana News - IndyStar is the home page of The Indianapolis Star with in depth local news, sports and entertainment.
philcafecomputing.tk Nepali having hot sex talk at bed. Pusy with hairs.
I want a girl who feels good about nepali having hot sex talk at bed herself also likes. Love to give oral well first of all im not a pusy with hairs slim nepali sex talk at bed having hot.
apartmani-omis.net Apartmani Omiš-Lokva Rogoznica-Ruskamen-Obriž-Pušić
Apartmani Pušić žele vam dobrodošlicu u mjesto Ruskamen kraj grada Omiša, mjesto na kojem se susreću more i sunce, čovjek i priroda, kamen i maslina - sve što je potrebno za istinski odmor...
Keywords: Ruakamen
mommeetmom.com Hello Mamas | Meet Mamas Like You!
Hello Mamas allows you to meet moms in your area with similar interests and lifestyles. The site helps moms meet other local moms who get along with one another based on dating site-like criteria. If you are looking to meet local moms, trying to find...
momgenerations.com Mom Generations - Mom Fashion and Beauty - Creating a Stylish Life for Moms - Style for Moms, Mom Fashion, Kid Fashion, Mom Beauty, Mom Fashion Tips
Style for Moms, Mom Fashion, Kid Fashion, Mom Beauty, Mom Fashion Tips
mamajamba.com Savvy Moms | Educated Moms | Talented Moms | Professional Moms
The community of savvy moms, educated moms, talented moms and professional moms.
whoistashanelson.com Who Is Tasha Nelson – Helping You Make Wellness A Priority
Helping You Make Wellness A Priority
lil-miss.com Advice for Moms- Best Advice for New Moms and Moms to Be
Modern moms give advice for new moms at MISS Magazine. Browse advice for moms, both new and moms to be.
Keywords: new moms advice, best advice for moms to be, sadie grace lenoble, jetsetter clothing, maggie fox at little warrior
nymomsworld.com New York Moms Groups & Parenting | NY Mom's World
NY Mom's World is the go-to guide for new moms, expectant moms, moms of toddlers & big kids and also MomPreneurs! We offer great giveaways, parenting advice, events, support groups, NYC mom groups in Manhattan, Westchester & Long Island.
Related keywords for pusy of momharga mom and jo beautiful mom, real mom exploited moms, pusy mom indonesia, hindi mom stories desi mom, single moms in jhb and sugar mom, karachi moms pusy image, foto mom pusy, blak mzansi mom pusy, mom ka choot bangali mom, mom or mom ki behan ko jamkar choda


text:
Product Description
Lancome Galateis Douceur Gentle Softening Cleansing Fluid for face and eyes contains natural cleansing agents such as papaya and pear.
Helps remove dead skin cells and leaves your skin clean and translucent.
Additional Information
|Package Content
|50 ml
|How to use
|Apply to the face with circular movements, then remove with a cotton pad.


text:
Posted: 05/17/2007
Posted: 05/17/2007
In addition to standup appearances on Comedy Central and NBC, Jeremy Schachter has been a host/correspondent on Tru TV, Spike TV, Discovery Channel, and Animal Planet. He is also a member of the Arts and Entertainment Law Journal at the Benjamin N. Cardozo School of Law in New York City.
You can follow his career at www.jeremyschachter.com.
© 2015 Comedy Partners. All Rights Reserved. Comedy Central and all related titles, logos and characters are trademarks of comedy partners.