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AngloGold sells Namibian mineComment on this story
Johannesburg - AngloGold Ashanti will sell its entire interest in AngloGold Ashanti Namibia (AGAN) to a wholly-owned subsidiary of QKR Corporation Limited, the company said on Monday.
AGAN owns the Navachab open-pit gold mine near Karibib, western Namibia.
"The agreement provides for an upfront consideration based on an enterprise value of US110 million (about R1.2 billion) which will be adjusted to take into account AGAN's net debt and working capital position on the closing date of the transaction (closing date).
"The upfront consideration is payable in cash on the closing date. In addition, AngloGold Ashanti will receive deferred consideration in the form of a net smelter return (NSR)," the company said in a statement.
The NSR would be paid quarterly for seven years.
The transaction is subject to fulfilment of a number of conditions precedent, including Namibian and South African regulatory and third party approvals.
These were expected to be obtained over the next few months, AngloGold said.
"We are executing on our strategy to focus our efforts on assets of scale that drive value in the business," AngloGold Ashanti executive vice president of strategy and business development Charles Carter said.
"We're pleased to have reached agreement to sell Navachab for fair value in the midst of a difficult market. We believe that QKR is the right group to take Navachab forward."
QKR president Lloyd Pengilly said Navachab was the company's first acquisition.
He said QKR believed Navachab had significant potential.
AngloGold Ashanti would continue to operate Navachab until the closing of the transaction. - Sapa
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The Top 10 In Action Sports
01Kenton Cool And The Legend Of Everest
02The Martyn Ashton Interview: Meet the Man Who Got Back on the Bike
03Legends Behind the Lens: The Dan Milner Interview
04We Asked Surf Star Coco Ho For Her Step-by-Step Guide to the Basics
05The Tony Hawk Interview: How Is The Birdman Still Flying High After All These Years?
06We Toured Britain's Action Sports Hotspots With The New Ford S-MAX
07The Legend Of El Dorado: One Man's Obsession With Finding A City Made Of Gold
08I Am Legend: How Audiences – Real Or GoPro'ed – Can Affect Your Mind And Performance
09Watch A Freerunning King Defy The Laws Of Gravity In This Urban Assault On London
10Living On The Blood and Urine of Dead Sea Turtles: The Shocking Story of A Sailor Stuck in Hell
Dark Meadows Collection Redux Edit
A Snowboarding video by Mpora - 1,213 views
This first collection was many years in the mind and a few nights shooting. Felt it needed one more round of editing before we moved forward to Antique Futuristic. Thank you to all that supported, helped, and made it real. Pilots Matt Boudreaux Jack Thonvold Joe Mertes Scott Kulzer Filmed By Eric Schleicher Craig Williams Jake Durham Photography Ryan Taylor Cameron Strand Shot on Location at The Klocker Farm The Sandberg Farm The Kulzer Farm Track Beach House/New Year Thanks to Juhyo, Autumn Line, Matt Porter, Adam Porter, Kevin Castanheira, Brian Wolfe, Gustav Ohlsson, All the Shops, Snowboarder Mag Mary, VG Justin, Ashbury, Neff Headwear, Howl, Travis Peterson, Vinny, Garrett and Mary McKenzie. http://vimeo.com/theipproject

subset: commoncrawl


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True Religion Jeans
Denise Richards in True Religion
On a relaxing vacation with her daughters, Denise Richards was seen with them hanging out by the pool. She must need a vacation with all of the drama that happens with ex-husband, Charlie Sheen! Although, these shots of her on her cell phone don't look as if she's having very much fun in Maui!
She does look great in her True Religion shorts and a bikini top. Denise has worn True Religion quite a few times before, you can see how she's styled them before, here. You can buy True Religion jeans at Truereligionjeans.com.
Can You Guess Whose Butt This Is In True Religion?
Can you guess whose butt this is in a pair of True Religion jeans?
True Religion's Friends & Family Promotion
True Religion will be holding their first friends and family sale on December 2nd until the 5th. They will be offering you 20% off of your order with the code TRUEFRIEND being entered at the checkout. You can also print out the coupon above and show it at the True Religion store to receive the in store discount. The discount is only valid at True Religion retail stores and True Religion outlet stores. 20% is an amazing discount and it's right before the holidays! You can snap up some amazing deals!
Katie Price in True Religion
Ex glamour model Katie Price, posed for photos at the launch of her new fragrance "Precious Love" at Semichem in Belfast, Northern Ireland. Katie has always been one to have a really different dress sense but she looks a bit like a yeti to me here, I'm not a fan of this look at all. There is too much white fluff going on! She would look much better with heels and a jacket.
Katie's jeans are a pair of True Religion skinnies which look like they are not done up, the zip fly is completely spread apart. I'm not sure what's going on with that. Is anyone actually a fan of Katie's style?
Images courtesy of Wenn.com


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Updated 12/19/2014
The acres of flowers, lush lawns, and canyon views at this riverside hotel make it more than a place to rest your head, and you could throw a rock and hit the south entrance to the park. A variety of room choices include modern suites with large soaking tubs and vaulted ceilings, and some have direct access to the gardens; those on the second floor have a balcony overlooking the Virgin River. The beautiful grounds include a pair of two-tier waterfall hot tubs—one by the pool and the other close to the river—which are great for soothing your muscles after a day's hiking in the park. The Cliffrose Café serves breakfast, lunch, and dinner, and will provide picnic sandwiches to go.
281 Zion Park Blvd., Springdale, Utah, 84767, United States
435-772–3234; 800-243–8824
Website: cliffroselodge.com
Hotel Details:
Updated 12/19/2014
No Thanks


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Oysters are said to be an aphrodisiac, much like a bottle of red wine or a powerful brain visibly pulsing beneath a skull. Start the evening off right with this Groupon.
Choose Between Two Options
- $23 for $40 worth of elevated cuisine, valid Friday–Sunday
- $20 for $40 worth of elevated cuisine, valid Sunday–Thursday
- See the menus.
Silo Elevated Cuisine
Considering the farm-inspired name and the refined dining rooms—white tablecloths, towering wooden wine racks holding bottles filled from carefully polished grapes—you could be forgiven for assuming that Silo Elevated Cuisine is a steakhouse. But a look at the dishes emerging from the kitchen reveals a less-predictable beast. On a menu that stars Silo's signature chicken-fried oysters, sources of gastronomic inspiration range from southern (applewood-smoked bacon, shrimp-and-andouille white-cheddar grits) to European (red-wine and cognac sauces), with more than a dash of Asian influence.
This is especially apparent in seafood dishes—crusted with Sichuan peppercorn or sesame seeds—and duck, served with five-spice seasoning or in spring rolls. Accordingly, Frommer's has hailed the restaurant as "one of the best places for fine dining when you want something other than French food." The original Alamo Heights location was joined by a new outpost in 2007, whose space Texas Monthly called a "minimalist study in black and white illuminated by crystal chandeliers and silvery globes."
92% of 1,785 customers recommend
"For someone who's never been it can be confusing but in the back is free valet parking and it's apart of another restaurant called NOSH but you can go into NOSH and..."
"For someone who's never been it can be confusing but in the back is free valet parking and it's apart of another restaurant called NOSH but you can go into NOSH and they will escort you to silo the building is connected nosh looked very casual but when you go into silo in the elevator it's extremely elegant so I recommend dressing up it was so beautiful and the environment was so calm I recommend the silo surf n turf it was a beef tenderloin with roasted garlic mash potatoes and spinach and two large scallops very delicious the menu is pricey so stuff your wallet :)"
"Great food, great service and a great atmosphere"
1133 Austin Hwy
San Antonio, TX 78209
+12108248686Get Directions


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Sam Alex
Taste of Country Nights with Sam Alex, heard Monday through Friday on Townsquare Media stations nationwide from 7PM to midnight.
Taste of Country Nights with Sam Alex, heard Monday through Friday on Townsquare Media stations nationwide from 7PM to midnight.
LeAnn Rimes is back in the swing of things with a new, full-length Christmas album, Today Is Christmas, released in October.
Granger Smith hasn't always been proud of his Halloween costume choices — in fact, one year, he admits he really embarrassed himself. Smith recently came to the ToC Nights Studio and got real with us about that awful costume from his past.
It's going to be a big year for Shania Twain. Not only is the legendary country singer hitting the road for her final tour this June, but she is also turning 50 in 2015!
These days, Carrie Underwood is balancing preparing to be a mom with releasing new music ... by blending the two together, sort of.
Carrie Underwood and husband Mike Fisher have stayed busy choosing a name for their first baby, a boy, due next spring. The couple has found a few they're happy with.
Talk about making lemonade from lemons! Little Big Town's Karen Fairchild couldn't sing one afternoon, but instead of calling it a day she created one of the most memorable moments from 'Day Drinking,' the group's hit song from the just-released 'Pain Killer' album.
Jason Aldean's exciting 'Old Boots, New Dirt' album impacted Tuesday (Oct. 7), and the 'Burnin' It Down' hitmaker couldn't wait to chat about it. He stopped by the Taste of Country Nights studio to dish on the release, how it came to fruition and why he's a self-proclaimed "song Nazi."
Joe Nichols stopped by the Taste of Country Nights studio to chat with host Sam Alex about baseball, his new baby and getting fired from his late-night Djing gig.
Country artists are on the road a lot! While touring, many artists just want to make their tour bus or time on the road feel like home, and they do that by bringing a few special things with them.
The last 30 seconds of Brett Eldredge's new 'Beat of the Music' video finds the singer locking lips with a beautiful blonde. The scenes took multiple takes, and he tells Taste of Country Nights host Sam Alex he wasn't complaining.


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On Tuesday, December 1, the HRC Foundation will participate in this year's CrowdRise Giving Tower in honor of Giving Tuesday.Read Article
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- Have questions about health & HIV? Check out @HRC's Handbook to Understanding Health & HIV https://t.co/qEwKFOJZDv... via @hrc
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Sarah returns to the cobbles thinking that she can put what happened behind her. We spoke to Tina O'Brien about the next twist in the story!
Poor Sarah's head is about to explode!
Kylie's relieved at first to see Sarah in a happier frame of mind but David remains unconvinced.
Then, after one-too-many drinks in the Rovers, Sarah accidentally stumbles into the path of Tim's van which opens up a whole can of worms.
Did David have anything to do with her accident? Kylie thinks so!
But that's nothing compared to the shock revelation the Doctor has for Sarah - will there be no escape from Callum's legacy?
FAVOURITE QUOTES: Mary's Mother-isms
INTERVIEW: Tracy's Jealousy
Tonight on Coronation Street...
Craig panics that he's not ready to move his relationship forward with Caitlin.
She wants me to come around tonight, while her dad's away.
Kylie accuses David of causing Sarah's accident.
Meanwhile, Sarah reels as the enormity of her situation hits home.
Nick and Carla have something very important to tell Gail.
Nick proposes to Carla!
Carla Connor, will you take this ring as a token of our engagement?
Mother always said a swift kick to the unmentionables would do the trick but frankly I'm a lot more comfortable up at the throat end.
I wasn't allowed to watch films like that as a child… Mother wouldn't let me watch anything with robots. One of her little rules. Sex, violence or robots. Or talking animals… Avalanches, beach scenes, anything with Olivia Newton-John, volcanoes, monks, haunted vehicles of any kind.
Did I ever tell you Mummy was psychic? …Six weeks before Amanda Burton left Peak Practice, she saw it in the bottom of her Horlicks. Now that's a gift.
Mummy wouldn't go out after dark because of her fear of owls.
My mother always said there was something very Slavic about me… It wasn't a compliment. Whenever I wore a headscarf she said I looked like I should be queuing for beetroot.
I've always considered myself a student of life – although Mother reckoned I'd failed my entrance exam."
I once took a set of pliers to Mother's chaise longue but it didn't end well.
Mother was once struck by a bolt that had fallen off the undercarriage of a passing helicopter... She had trouble remembering some months of the year after that and she developed an irrational fear of the colour yellow, so that was it for custard in our household. And she called me 'Daphne' occasionally, which was odd because she didn't know any Daphnes."
Whenever anyone used the expression 'Always the bridesmaid, never the bride,' my mother used to say, 'Our Mary'll never even make bridesmaid.' She was right."
I'm turning into my mother. What a terrible thought. 'Complicated woman' is a polite way of putting it.
We last saw the dastardly Pat Phelan riding off into the sunset having blackmailed Anna into sleeping with him and swindling her family out of their life savings.
How will Anna and Gary react to see the bothersome builder back on the cobbles?
Connor McIntyre started filming this week and will be back on screens in the new year!
Tonight on Coronation Street's hour-long episode...
Sarah can you hear me?
Tonight on Coronation Street...
Sarah returns home but will she be welcome?
It's not going to be fine...
We put our pedal to the metal and joined the cast and crew as they got the behind the wheels of Luke's car race.
What happens if you put Dean Fagan, Tisha Merry, Oliver Farnworth and James Atherton out on a freezing street in the middle of Manchester?
Car-nage! (sorry)
Dean talks about stunt drivers and sitting in the driving seat, James reflects on playing the villain and see how we filmed the heart-pounding race - including drone-cam!
EXCLUSIVE: Mikey North on Gary's return
PICTURES: Nick proposes to Carla!


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Tagged: Tempzone
The Cost of Radiant Heated Floors is Now More Affordable
Living rooms that need a flooring upgrade are great choices for in-floor heating. WarmlyYours offers affordable radiant heating options that will suit any room.
Read Post
WarmlyYours Fourth-Quarter, Full-Year 2014 Report Shows Growth
WarmlyYours reveals its performance for fourth-quarter and full-year 2014. Outdoor heating solutions and mirror defoggers stood out as some of the fastest-growing product lines last year.
Read Post
Radiant heated floors cost very little to spruce up a small bathroom
Adding affordable radiant floor heating to a small bathroom can enhance its value, style and comfort.
Read Post
Homeowner Uses Humor, Helpful Tips to Install Heated Floors
Phil Brind'Amour describes how he installed a WarmlyYours' radiant floor heating in the first-floor bathroom of his old farmhouse.
Read Post
In the Kitchen: Low Cost of Adding Radiant Heat
Installing heated floors in a large kitchen is an affordable way to keep family and friends comfortable.
Read Post


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Shannon Terry is CEO of 247Sports.
- Filed Under
247Sports has partnered with coaching news site, CoachingSearch.com, to expand its coverage of college and professional coaching moves.
CoachingSearch.com owner Pete Roussel will join Brentwood-based 247Sports, a network of about 65 recruiting websites, as part of the deal, according to a release.
"Since our launch in August 2010, 247Sports has been aggressively building a footprint in team news and recruiting scoop," 247Sports CEO Shannon Terry said in the statement. "It is a natural progression to make a greater investment in the rapidly growing coaching news and transaction ...


text: Blog For Someone, Not About Something… By Kevin McKeown on March 24, 2015 Posted in Business Development, Relationships, Social Networking When I start a blog post, I do so with a specific person in mind. I think about that one reader that I know will read my blog post. Thinking about and connecting with just one reader helps me build momentum as a writer. I often end up with a solid draft post. At that point, … Continue Reading


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Welcome to Belfast Bupa CentreOur Belfast centre is run in partnership with BioKinetic Europe and is centrally located within walking distance of bus and train services. There are several public car parks in the area.
We offer a range of health assessments. These offer insight into your current health and potential future health risks and can help highlight any necessary lifestyle changes.
Centre open today: 8:00am to 4:30pm
0345 600 3458 ^
Lines are open 8am to 8pm Monday to Thursday, 8am to 6pm on Friday and 8am to 1pm on Saturday
Services and treatments available
Advanced Health Assessment
Our most comprehensive health, fitness and lifestyle assessment. This is designed to give you a thorough understanding of your overall health, fitness levels and potential health risks.
More about Advanced heath assessment
At a glance:
150 minutes maximum duration
39 tests and measures
up to 45 minutes with a Bupa doctor
Complete Health Assessment
The all-round health assessment. This health and lifestyle assessment gives a detailed picture of your state of health and potential future health risks.
More about Complete health assessment
At a glance:
120 minutes maximum duration
32 tests and measures
up to 45 minutes with a Bupa doctor
Essential Health Assessment
The Essential assessment is available for anyone aged 18-65, however is designed especially for those under 45. It assesses your current health and helps to identify any potential future health risks.
More about Essential health assessment
At a glance:
60 minutes maximum duration
17 tests and measures
up to 25 minutes with a Bupa doctor
Mature Health Assessment
If you're over 65, get a picture of your health and potential future health risks. This is a two hour assessment, including age and gender specific tests
More about Mature health assessment
At a glance:
120 minutes maximum duration
up to 60 minutes with a Bupa doctor
Female Health Assessment
This assessment involves a thorough health check, including a breast check, smear test and allows the opportunity to discuss any health concerns.
More about Female health assessment
At a glance:
60 minutes maximum duration
up to 30 minutes with a Bupa doctor
Breast health check
Our breast check involves a series of tests designed to help to identify changes or abnormalities in your breasts. The mammogram part of the breast check is usually only available for women aged 45 and over, in accordance with the NICE guidelines. However if you have a clinical indication of risk or a family history of breast cancer you may be eligible to be tested. If you're having a breast health check as part of a health assessment, it may be delivered at a different location. Please contact us for more information.
More about Breast health check
GP services
We provide private GP appointments, and can usually provide an appointment on the next working day, if not sooner. Customers must be 18 years and above.
More about GP services
Seasonal flu vaccinations
Minor illnesses such as flu are one of the main reasons for short-term sickness in the UK. Help protect yourself and others in the colder months with a seasonal flu vaccination available at our centre.
More about vaccinations
Opening times
- Monday 8:00am to 4:30pm
- Tuesday 8:00am to 4:30pm
- Wednesday 8:00am to 4:30pm
- Thursday 8:00am to 4:30pm
- Friday 8:00am to 12:30pm
- Saturday Closed
- Sunday Closed
What's next?
Request a callback
Make a booking enquiry
^Calls may be recorded and may be monitored
Bupa Health Assessments, Occupational Health services, 360 Health Risk Management services and Absence Management services are provided by Bupa Occupational Health Limited. Registered in England and Wales No. 631336. Registered office: Bupa House, 15-19 Bloomsbury Way, London WC1A 2BA.


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Beyond the Grave
How visions of what awaits us after death have changed across the ages
'O you who are still on earth and pass this stone, you who love life and hate death, make offerings of fruit, fowl and meats to Osiris on behalf of the child buried here." This anonymous utterance from beyond the grave, inscribed on an Egyptian funeral stone some time between 2050 B.C. and 1650 B.C., is one of the more haunting testimonies included in "After Lives: A Guide to Heaven Hell, and Purgatory." British scholar John Casey's wide-ranging study shows the many ways in which mankind has tried to make sense of life after death.
Exploring beliefs about postmortem existence from ancient Egypt, Mesopotamia, Israel, Greece and Rome—as well as from the theologies of Christianity and Islam—Mr. Casey discovers no broad pattern or shared assumption. According to anthropologists, the earliest cultures appear to have feared the dead more than death, burying corpses bound by rope and hemmed in with heavy stones for fear they might return and terrorize the living. For the Homeric Greeks death itself was the cause of dread, even as it might bestow honor. "Throughout the Iliad we have warriors who are reduced to nothing as their soul flees to Hades," Mr. Casey writes. "It is as though human greatness must include a sense of the nothingness that awaits us, and that the hero have a clear sense of this nothingness."
The ancient Egyptians were the first people who developed fully worked-out beliefs about personal immortality and who connected an afterlife with good and evil conduct in this world. Thanks to mummification, the corpse could lie uncorrupted in the tomb and be reunited with its spirit after death. What began as an afterlife available only to the pharoahs quickly democratized into a widespread belief in the possibility of resurrection for everyone. Intensely loving life, the Egyptians imagined unapologetically physical rewards for the risen: "I walk upright," one anonymous writer's expectations went. "My phallus is united with me. My anus is united with me. I eat with my mouth. I have a motion with my anus."
The Christian mythology of hell-fires and heavenly angels appears to start with "The Apocalypse of Peter," an apocryphal biblical text dating from the first half of the second century B.C. Its themes were amplified by writers such as Tertullian (160-225). Mr. Casey has little sympathy for what he calls the "extreme crudity and silliness" of these attempts to terrify a heathen audience into renouncing its worldly ways. By contrast, he argues, Dante's "Divine Comedy," from the early 14th century, is the most psychologically convincing portrayal of heaven and hell, largely because Dante followed Aquinas in joining the moral psychology of Aristotle with Christian doctrine.
Dante also described Purgatory. It had very little scriptural warrant but which greatly increased the spiritual influence of the Roman Catholic Church (because one's fate was not settled at death) and gave rise to many abuses, since the soul's time in Purgatory could supposedly be shortened by the earthly sale of "indulgences." Yet Mr. Casey argues that Purgatory—introduced in the 13th century—was Rome's "happiest inspiration." The whole idea of a place between heaven and hell, where sins might be punished but also ultimately atoned for, widened the possibilities of salvation and made Christian after-life judgment more humane.
The New Testament's pronouncements on salvation, Mr. Casey notes, were often ambiguous or at odds with one another, supporting different interpretations. The Church Fathers thus tried to codify the proper Christian view of what death might (or might not) bring. "Augustine's doctrines of original sin," Mr. Casey writes, "and the consequent depravity of human nature, of the absolute necessity of God's grace in procuring man's salvation, and above all his unrelenting insistence upon a doctrine of predestination have indelibly marked all subsequent theology." The writings of St. Augustine (354-450), he says, were particularly influential in the 16th century on the thinking of John Calvin, "whose own account of predestination molded and terrified millions through the Protestant centuries."
After Lives
By John Casey
Oxford, 468 pages, $35
Augustine won out in his battle against two early Christian thinkers, Origen and Pelagius, who were declared heretics for suggesting that moral self-help could co-exist with divine grace as a means of gaining salvation. Mr. Casey notes an irony: The Vatican has never formally repudiated predestination, but the church "now in practice allows the faithful to be as cheerfully and unconsciously Pelagian as everyone else." And "everyone else" is just about right when it comes to the U.S. A recent Gallup survey reported that 71% of Americans believe in heaven and that 93% of them think they have an excellent, good or fair chance of getting there.
Whether what awaits them is an 18th-century gentlemen's club, or a place of conjugal love, or a Muslim paradise of the senses, we don't know, but all are well-documented theories. "After Lives" catalogs them with mischievous pedantry. Mr. Casey reports that the 19th-century philosopher Emanuel Swedenborg even claimed to have visited heaven, where he conversed with angels. A version of conversation was part of the spiritualism of the early 20th century, as Mr. Casey recounts: Table-tapping psychics met the emotional needs of a generation of otherwise intelligent men and women who had lost children in World War I.
"After Lives" has its shortcomings. It is over-long in places and often feels like a synthesis of secondary sources. The book's pugnacious style, although immensely readable, may infuriate specialist scholars more used to disinterested debate. Most disappointing, Mr. Casey pulls his final punch. Concluding that "our image of heaven and hell is finally an image of how we judge ourselves," he suggests that the dismissive attitude of "educated moderns" toward today's apocalyptic Muslims and evangelical Christians may reflect a lack of self-belief about their own values. What these values could be is left tantalizingly blank. Still, "After Lives" offers a humane and often witty engagement with the concepts of heaven and hell in different times and according to different faiths. It is ironic that a book about life after death so enlivens one of the most important conversations we can have in the here and now.
—Mr. Archer is a writer in London.


text: When Darren Criss came to Billboard's studios for a live Q&A on April 26 to answer questions from his many listeners, he was also kind enough to sign four copies of his Billboard cover issue to give away to fans. Want to be one of those lucky four? Just fill in the form below by May 26, 2011.


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Where Are You Located?
Family Montessori is currently located at 220 East Main Street directly next to St. Patrick's Church. We are very blessed to be holding classes in this lovely space through the generosity of St. Patrick's Church this year. Our classroom can be reached by using the parking lot behind St. Patrick's Church and following the sidewalk into the fenced-in courtyard area where the two classrooms are on the first floor. The first door is for the 6-9 classroom. The second door is for the 3-6 classroom.
When Can We Enroll?
Enrollment for Family Montessori's 2015-2016 year is now open to the community. We do anticipate filling both classes so we will accept applications on a first come, first served basis. For students that apply after all spots are filled, we will be maintaining a wait list for those interested. These students will also be served on a first come, first served basis.
Program Offerings & Tuition for the 2015-2016 school year:
- Preprimary & Kindergarten: 3 days/week (W/Th/F) from 9:30-12:30
- Tuition: $3,500
- Preprimary & Kindergarten: 5 days/week** from 9:30-12:30
- Tuition: $4,600
- Lower Elementary: 3 days/week (W/Th/F) Homeschool (M/T) from 9:30-3:00
- Tuition: $4,500
- Lower-Elementary: 5 days/week** from 9:30-3:00
- Tuition: $6,700
** The 5 days/week option must have a minimum number of students enrolled in order for the program to be held.
Sliding Scale Tuition Option
Because part of our mission is make a Montessori education available for most everyone, we are pleased to be able to offer a Sliding Scale Tuition Option based on family income and size. To determine your tuition rate based on the Sliding Scale Option, please email the following information to our Business Administrator, Lisa O'Bryan at email@example.com. Once she has your information, she will be able to tell you your adjusted tuition rate for the 2015-2016 school year.
- Name(s) and age(s) of children to be enrolled
- Which program they would attend
- Number of persons in the family
- 2014-2015 Adjusted Gross Income, line 37 (income verification due with enrollment contract)
Please note that this adjusted tuition rate is just a quote for your purposes of determining your decision to enroll in Family Montessori and is not at this a binding quote. Your information will also remain confidential.
How Do I Enroll?
- Please call 513-800-8023 to get an Application for Admission for the 2015-2016 School Year.
- Next, to see if you qualify for our Sliding Scale Tuition Option, please email our Business Administrator, Lisa O'Bryan, at firstname.lastname@example.com and provide her with the information detailed above to see if you qualify. She will respond with your adjusted tuition rate based on your submitted information.
- Mail in your Application for Admission along with a non-refundable check for the $50 application fee (per family) to: Family Montessori of Lebanon, P.O. Box 562, Lebanon, OH 45036
- Receive a contract either by mail or personal meeting which reflects your tuition rate.
- Sign and return contract.
Once your child is enrolled, you will be given additional paperwork that can be turned in prior to the first day of school. All children in the 3-6 classroom will need to have a Medical Statement Form signed by their pediatrician.
Please feel free to call us with any questions you have about this enrollment process. You can reach us at these numbers:
Erica Nichols, Executive Director, 6-9 Teacher
Lisa O'Bryan, Business Adminstrator
Tina Evans, Director of Preprimary & Kindergarten, 3-6 Teacher
State Guidelines
Elementary families wishing to participate in the Family Montessori Homeschool Option will need to officially register their child for home education by notifying the district Superintendent.
Based on state home school requirements, the parent takes ultimate responsibility for curriculum and education. However, our full-time, certified teacher works to assist families in making sure that Ohio curriculum standards are met as well as the requirement for 900 hours, both in and out of the classroom.
According to ODE guidelines, at the end of each year, the home school parent must have the child evaluated by either a standardized test, portfolio review by certified teacher or per agreement between the parent and the Superintendent.
ODE home education facts at: http://education.ohio.gov/GD/Templates/Pages/ODE/ODEDetail.aspx?page=3&TopicRelationID=698&ContentID=5223&Content=104696
- Family Montessori of Lebanon, Inc. admits students of any race, color, and national or ethnic origin.


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Work to close Fort Duquesne Bridge ramps
PennDOT crews will close ramps to and from the Fort Duquesne Bridge nightly this week, officials said.
From 10 p.m. Monday to 5 a.m. Tuesday, the ramp from the Fort Pitt Bridge to Fort Duquesne Boulevard will be closed; traffic will be detoured via Liberty Avenue and Stanwix Street.
On Tuesday night, the ramp from the Fort Duquesne Bridge to Fort Duquesne Boulevard will be closed. On Wednesday night, the ramp from the 10th Street Bypass to the Fort Duquesne Bridge will close.
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PIERRE, SD — The Oahe Hockey Association (OHA) invites boys and girls ages four to nine years old to the ExpoCenter in Ft. Pierre on Saturday, November 2nd for a Try Hockey for Free clinic from 10:30 a.m. to noon. At this free event the OHA will provide skates, helmets and sticks for the young skaters to experience the sport of ice hockey in a fun and safe environment. Certified coaches will teach the basics of the sport to participating kids, and OHA volunteers will provide information about the youth hockey program to interested parents. Kids who attend will also be entered into a drawing to ride the Zamboni to clean the ice at the end of the clinic. Families can register in advance at www.TryHockeyForFree.com. The first 40 kids to register will receive a free jersey. Kids should wear loose fitting sweat pants or jeans, winter gloves or mittens, and a sweatshirt or jacket for the clinic. The OHA is partnering with the National Hockey League and USA Hockey to offer the free clinic as part of a nationwide effort to introduce kids to the sport of ice hockey. More than 500 local rinks throughout the country will host a Try Hockey for Free clinic on November 2, 2013. For more information about Try Hockey for Free Day, contact Laura Schoen-Carbonneau at 605-222-8639or firstname.lastname@example.com.
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Performance golf ball fitting focuses on finding the best ball for your game and lowering your score.
Be the first to hear about product introductions, surveys, promotions, and sweepstakes. Through Team Titleist News we will provide exclusive communication not available in any other forum.
Titleist offers the most precise club fitting experience in the game.
The full-set Titleist Golf Club Trial program provides golfers with an easy way to experience Titleist products on either the course or practice tee to help determine the right equipment for their game.
Track your stats. Get video tips. Set goals for your golf game.
Started by :
alex m
3 years ago
3 years ago
Neither can we Alex. Remember to keep your profile up to date because we will be inviting Team Titleist ANZ members out to some special preview days to try the new 913 before it goes to stores.
Thanks for your note.
When and where will that be ? Saving up to get one, can't wait
Are the AP2 coming out aswell with the 713 ? Or should i just buy the AP2 712 this week, the buy the 913 in november ?
Hi Joshua,
About how Titleist name our golf clubs.
913 is the series designation for the new family of metals. Titleist continues to release metals under the 900 number designation with the "9" standing for metals and the "13" indicating the new model year, 2013.
The same is for the irons - 712 is the series designation for the family of irons released in 2012. etc and previous models 710 (2010).
There are no new Titleist iron models scheduled for release in November 2012.
Hope that clears up any confusion.
Just played my first round with my brand new 913D2 driver :) it is crazy good :) i love it to bits and cant wait to get its brothers and sisters in the 913 fairways....worth waiting for :)
Hi there, I recently bought a Titleist driver 913 9.5 and I'm very happy with it. I was however wondering how good or genuine is the Diamana Ahina 72 D+ shaft that came standard with it. I did tell the fitter that I ant a low flight low spin shaft and tha's the one that he recommended.
As I said I'm very happy with it but I was recently looking at the Motore Speeder Tour spec VC7.2 and was wondering how does it compare with that has anyone hit with both. The MOtore is a very expensive shaft and it's hard to get it up here on the Gold Coast to try out, so I was wondering how does it compare and how good or genuine is the diamana shaft. In some blogs I read that a real diamana is $300???
i want to get the sáme
I've previously been told at a club fitting - the cost of the shaft is only an indication of how many manufactures use the shaft as stock v's those who have an up-charge. It's a supply and demand game really.
I've gone from Diamana to Motore. Very happy
i just got titleist 910d3 910h and 910f love them alot. Simply are the best!!
2 years ago
these drivers are the best out


text:
A wicked little collection that really lives up to its name – a set that's filled with warmth and good energy throughout – not to mention a great array of overlooked soul tracks too! Most of the numbers here are lesser-known gems from the late 70s and early 80s – upbeat, but not in a disco way – and usually somewhere in a space between modern soul and boogie, which works perfectly for the positive vibes of the tunes! There's a soaring feel that definitely takes off from an Earth Wind & Fire sort of style – and although most of the artists here are familiar, you'll really be surprised at the tracks chosen for the set. Titles include "Love Vibes" by The Emotions, "Something On Your Mind" by Melba Moore, "Razzle Dazzle" by Heatwave, "Better Things" by Sylvia St James, "Riding" by Michael Henderson, "Can You See Where I'm Coming From" by Instant Funk, "All In The Way You Get Down" by Ray Parker Jr & Raydio, "Sweet Fire" by Mighty Fire, "Here I Am" by Dynasty, "No One" by Flakes, "Love Is Such A Crazy Thing" by The Spinners, "Wonderful" by GQ, and "When I'm Dancin" by Lenny Williams. © 1996-2015, Dusty Groove, Inc.
We realize that there are many different interpretations of the standard grades used for pre-owned vinyl record albums & CD, so we thought we'd offer you the ones that we are working with, so you have an idea what we mean when we give the grade for a non-new item on our pages.
Below are stated conditions for a used vinyl records at Dusty Groove. Grading for the cover should be assumed to be near (within a "+" or "-") the grading for the vinyl. If there is significant divergence from the condition of the vinyl, or specific flaws, these will be noted in the comments section of the item. However, please be aware that since the emphasis of this site is towards the music listener, our main concern is with the vinyl of any used item we sell. Additionally, please note that all of our records are graded visually; considering the volume of used vinyl we handle, it is impossible for us to listen to each record. If we spot any significant flaws, we make every attempt to listen through them and note how they play.
The following grading conditions apply to the vinyl component of an album or single:
This is what it says, that the record is still held fast in shrink-wrap. We tend to be pretty suspicious about these things, so if the shrink-wrap doesn't look original, or if the record seems to have undergone some damage over time, we'll probably take it out of the wrapper to ensure that it's in good shape — which is why we don't have more of these. In some cases the shrink-wrap may be torn in spots, but if it's not possible the record has been taken out and played, the record will still qualify as "Sealed".
As a rule, Dusty Groove does not use the grades of Near Mint (or Mint, for that matter) because in our experience, we find that no records ever qualify for such a high grade. Even sealed records tend to have one or two slight faults, enough to usually qualify them for a grade of NM- or lower. We've often found that records which are clearly unplayed will have a slight amount of surface noise, especially in quieter recordings.
This is a grade we rarely use, as we try not to sell records in very bad condition, though in some rare cases we will list a record in such bad shape that it does not conform to the standards above. A "Fair" record will have enough marks or significant flaws that it does not even qualify as "Good", but is a copy you might consider for playing, if you're willing to put up with noise and/or flaws. An example might be a recording with surface noise so heavy that it is equal to the volume of the music. For records listed as "Fair", we will describe the extent of the condition in the comments.
Like "Fair", we rarely list records in this condition, as they represent the extreme low end of spectrum. These records typically have multiple serious problems, and we offer them as "relics" or "objects" only — for those who want to at least have a copy of a record, even if it is not really worthy of play, perhaps for the cover alone. For these records, we will describe the extent of the condition in the comments.
If something is noteworthy, we try to note it in the comments — especially if it is an oddity that is the only wrong thing about the record. This might include, but isn't limited to, warped records, tracks that skip, cover damage or wear as noted above, or strictly cosmetic flaws.
We have only one grade for non-new CDs at Dusty Groove — "Used CD". This grade is somewhat all-encompassing, but we choose it because we try to offer Used CDs in the best shape possible. All of our Used CDs are guaranteed to play without skipping or flaws. If you purchase a Used CD from Dusty Groove, you have 1 week to play it to determine that it plays correctly — and if it does not, then you may return it for a full refund.
With our Used CDs, you can expect the disc to be free of all but the lightest of surface marks — clean, and not dirty at all. You can also expect the case to be clean (we often change the cases ourselves — putting fresh cases on Used CDs we handle) — and you can expect the booklet to be in good shape, unless noted otherwise. We will list any specific details/defects underneath the item — so look for notes on cutout marks in the case, stamps on the barcode, or details like that.


text:
Page 1
Volume 37, Issue 5, May 2009
Online English edition of the Chinese language journal
Cite this article as: Chin J Anal Chem, 2009, 37(5), 758–763.
Received 2 August 2008; accepted 20 November 2008
* Corresponding author. Email: firstname.lastname@example.com
This work was supported by the Guangxi Science Foundation of China (Nos. 057027, 0718007b-30).
Copyright © 2009, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences. Published by Elsevier Limited. All rights reserved.
Application and Progress of Raman Tweezers in Single Cells Application and Progress of Raman Tweezers in Single Cells
AI Min1,2, LIU Jun-Xian1, HUANG Shu-Shi2, WANG Gui-Wen2, CHEN Xiu-Li1,2, CHEN Zhi-Cheng1,2,
YAO Hui-Lu2,*
1 College of Physics and Electronic Engineering, Guangxi Normal University, Guilin 541004, China
2 Lab of Biophysics, Guangxi Academy of Sciences, Nanning 530003, China
Abstract: Raman tweezers is a new optical technique that combines laser tweezers with Raman spectroscopy, which has the
capability of studying single biological cells or organelles in nearly natured solution. Owing to its characteristics of noncontact,
noninvasion, fast identification, and real-time detection, it has found application in improving the Signal Noise Ratio of Raman
spectroscopy, optimizing the detection and sorting single cells or organelles, practicing the real-time detection of biochemistry
dynamics process so as to make the activity and mechanisms of biomolecules understood deeply. The source, principle of Raman
tweezers, and its optical application in biology are depicted and reviewed particularly in this article.
Key Words: Raman spectroscopy; Optical tweezers; Single cell; Raman tweezers; Review
1 Introduction 1 Introduction
Raman spectroscopy is based on the inelastic scattering of
photons due to the vibrations of molecules or crystal lattice
level. It is named after the famous India physicist C.V.
Raman who first found the famous phenomenon of the
Raman effect in 1928[1]. It is one of the most powerful
analytical techniques in the different areas of physics,
chemistry, biology, and corresponding interdisciplinary
branches of science. Coupling Raman spectroscopy with a
confocal optical microscope (micro-Raman spectroscopy),
one can achieve chemical characterization on a scale of
femtoliter volumes within the sample to be probed. But this
technique acts only on the sample immobilized in experiment.
However, there is a large class of objects of micrometer sizes,
in particular living cells (blood cells, yeast cells, for instance),
which keep normal in a liquid environment. Both the above
mentioned objects are extremely important. Cells are parts of
living organisms. However, Brownian motion and motility
(for some living cells) in the surrounding medium prohibit the
direct use of the Raman technique and can acquire the
comprehensive information without combining it with an
immobilization technique.
A micropipette or chemical methods could be applied to
living cell immobilization on planar surfaces. However, in
many cases, this may induce the undesirable perturbations of
chemical reactions in living cells. Chemical immobilization
can change the chemical microenvironment of living cell and
may yield other unknown effects. And there is the limitation
of the micropipette. Surface effects become more important
as the size of the microobject decreases. It will induce the
membrane change of cells and destroy the normal shape of
cells by micropipette in experiment. The analytical
capabilities of Raman spectroscopy are limited by its inability
to manipulate and therefore thoroughly analyze the biological
molecules under study without making physical contact. This
limitation has been solved by optical tweezers' invention and
improvement gradually[2,3].
As early in 1984, Thurn et al realized that using only the
force of radiation pressure successfully trapped microsized
solid particles in stable optical potential wells and excited
Raman spectra of them called Raman microprobe[4]. However,
it brought photodamage to the living cells because they
absorbed the energy of visible light. Thus, the method of
coupling Raman spectroscopy with optical tweezers was used
in aerosol particles, chemical reactions in microdroplets, gas
Page 2
AI Min et al. / Chinese Journal of Analytical Chemistry, 2009, 37(5): 758–763
bubbles, and other nonliving particles earlier[5?7]. The optical
tweezers, which was first developed by Arthur Ashkin et al of
Bell Laboratory in 1986, has the ability to control objects
ranging in size from 5 nm to over 100 mm, whether they are
atoms, viruses, bacteria, proteins, cells, or other biological
molecules[8,9].The essential process of life depends on the
intracellular harmonious actions between many molecules and
similar molecules. If human beings expect to understand the
mechanisms of life, they must mastery the activity of
biological molecules based on the single-molecule level and
then intervene and embellish it to serve life sciences.
However, a laser used for Raman spectroscopy should be a
continuous wave with a power to trap and control the particles.
The wavelength and power of the laser are the most important
criteria for determining both the viability of the sample during
measurements and the ultimate signal noise ratio (SNR).
There is photodamage to cells brought by laser in early study
due to the limitation of technical levels of the day. Until 2002,
Ajito et al combined the optical tweezers with micro-Raman
spectroscopy and first successfully identified single living
biological ones from many organelles[10]. In another study
published almost simultaneously, Xie et al used near-infrared
laser with a wavelength of 785 nm for capture laser and
developed a laser tweezers Raman spectroscopy (LTRS)
system, which obviously reduced photodamage. Then, this
group first observed the biochemical dynamics process of
single trapped living cells[11?13].
Raman tweezers is an optical technology to couple optical
tweezers with Raman spectroscopy, also called the Raman
spectroscopy combined with optical trap (RSOT) technique or
the laser tweezers Raman spectroscopy (LTRS) system[11,14?17].
It can fix the sample in the suspending medium by optical
tweezers. This optical immobility technique can control the
sample without physical contact or absorption of it to a
surface or addition of any chemical agent to immobilize it. It
has realized the detection of Raman spectra of living cells in a
long time nearly without damage, conquered the limitation of
traditional Raman spectroscopy in the immobility of living
cells, and improved the activity of biological single cells in
experiment. Recently, the studies of Raman tweezers have
widely emerged in today's world rapidly, such as the studies
in American East Carolina University, University of
California, United States Army laboratory, Spanish ICFO,
and so on. As a new powerful technique is developed
constantly, more and more people have an interest on it.
2 Brief introduction of Raman tweezers 2 Brief introduction of Raman tweezers
Raman tweezers is a relatively new technology that couples
Raman spectroscopy with optical tweezers to achieve the
previously unheard control and resolution of sample. A laser
beam is introduced into an inverted microscope through a
high numerical aperture objective to form an optical trap
termed optical tweezers[18]. The optical tweezers can trap the
particle in three dimensions nearly in the balance of the focus
of a laser beam. The same laser beam is used to excite Raman
scattering of the trapped particle. The scattering light from the
particle is collected by the objective and coupled into a
spectrograph (Fig.1)[19].
A diode laser (DL) beam passes through an interference
filter (IF), a pinhole (PH), and then enters into an inverted
microscope through a dichroic mirror (DM) to form an optical
trap. The Raman-scattering light of the trapped particles
collected with the same objective passes through a
holographic notch filter (HNF1) and a pinhole (PH), and then
it focuses onto the entrance of an imaging spectrograph
equipped with a CCD detector. M denotes reflection mirror, L
denotes lens, BS denotes beam splitter, Obj denotes objective
lens, EP denotes eyepiece, VC denotes video camera; Lamp
denotes Lamp-Green-filtered xenon illumination light [19].
The combination of the two techniques makes it have two
advantages of both optical tweezers and Raman spectroscopy.
Biological cells contain complex mixtures of biomolecules
enclosed within cell membranes. These biomolecular include
nucleic acids, proteins, polysaccharides, and lipids[13].
Understanding various cellular processes requires identify
these molecules inside the living cells. Raman spectroscopy
provides a convenient means of attaining this information
because of its ability to detect and identify molecules in
microregions individually. Valuable information about the
composition, secondary structure, and interactions of
DNA-protein complexes inside living cells can be attained
from the position, intensity, and line width of the Raman
peaks in the spectra[17]. This optical trap also allows Raman
tweezers to easily separate molecules for isolated studies,
such as their response to different conditions and/or
treatments. Optical tweezers provides the further advantages
of eliminating stray light and fluorescence, as well as holding
a molecule in place in an optical trap, allowing for the best
possible excitation and collection of Raman spectra[11].
Fig.1 Experimental setup
Page 3
AI Min et al. / Chinese Journal of Analytical Chemistry, 2009, 37(5): 758–763
The results of Raman tweezers are depicted in the form of
Raman spectrum profiles. Each peak on the spectrogram
represents a particular molecule (DNA, amino acid, amide,
for instance) in the sample. The Raman shift is different for
every unique molecule, thereby allowing Raman tweezers to
create spectroscopic "fingerprints" of molecules that can be
used as reference in analytical studies. Many treatment
methods and arithmetic are widely used in the classification
and identification of spectra data, such as principal
components analysis (PCA), discriminant function analysis
(DFA), hierarchical cluster analysis (HCA), two-dimensional
correlation analysis, multivariate linear regression (MLR),
and artificial neural networks (ANNs)[11,19?21].
In this article, the progresses of Raman tweezers are
described, especially in the application of medicine diagnosis
and the monitoring of biochemical dynamics process. As a new
field, it requires continuous development in both the technique
and study of essential problems of cell molecular biology.
3 Applications of Raman tweezers 3 Applications of Raman tweezers
3.1 Cancer and virally infected cells3.1 Cancer and virally infected cells
The Raman spectra of cancer cells have been studied for
decade years, which are resulted from the mean of the cell
colony information. Also, the process of identification of
normal or cancer cells is laggardly. How to quickly diagnose
whether the tissue is cancerous has gained increased interest
among doctors and investigators recently. The tissue is
cancerous mainly due to the changes of the components and
structure of cells, such as protein, nucleic acid, lipid, and
others. Yao et al[22] studied the Raman spectra of single
normal airway epithelial cells and carcinoma of nasopharynx
cells and found obvious difference between both the spectra.
They could be distinguished by PCA or DFA drastically
(Fig.2). This technique was also used to study rectal cancers[23]
and Kaposi's sarcoma-associated herpesvirus (KSHV)[15].
Raman tweezers, while yet far from having proven to be one
of the most effective analytical tools to identify quickly
normal and cancer or virally infected cells, is still in infancy.
With proper nurturing, this technique has the potential to
blossom into a high useful tool in the doctors' arsenal.
3.2 Organelle or subcellular structure 3.2 Organelle or subcellular structure
In cell biology, an organelle is a discrete cellular structure
having specialized functions. Ajito et al[10] used Raman tweezers
to distinguish aggregated and single synaptosomes in the optical
trap. It has the analytical capabilities of distinguishing quickly
the structure of cells, which other techniques cannot. Ojeda et
al[24] found that Raman tweezers has the capability of sorting out
chromosomes. With respect to the traditional method of
G-banding, the advantages of Raman tweezers are noninvasive
and lack of sample preparation. Tang et al[25] first studied the
Raman spectra of intact and swollen mitochondria induced by
calcium ions to analyze the chemical composition changes of
them. It was found that the band at 1602 cm?1 changed very
rapidly during experiment. Thus, the band at 1602 cm?1 can be
the biomarks of single living mitochondria. Compared with
fluorescent label, the characteristic band as a bioprobe label is
noncontaminative and noncontact to cells. As such, Raman
tweezers has the analytical capability to study living samples at
the deeper level of organelle or subcellular structure. For further
researches, its potential capability cannot be ignored in the
phenomenon detection (for instance, cell communication and
cell signal transduction, the mechanism of membrane channel)
of molecule biology.
3.3 Stress response of red blood cell 3.3 Stress response of red blood cell
One of the central problems in cell biology is the
understanding of cell responses to the changes in
environmental conditions. Cell stress response is the term for
the reaction of a living cell to ambient changes, which are
potentially harmful, for example, an increase in temperature,
the pH, the saline concentration, or the presence of toxins.
Yue et al[26] selected different direct currents ranged from 0 to
15 mA to act on different red blood cells (RBCs).
Fig.2 Analyses of single-cell averaged spectra with PCA (a) and DFA (b)[22]
Page 4
AI Min et al. / Chinese Journal of Analytical Chemistry, 2009, 37(5): 758–763
Fig.3 Comparison of NIR Raman spectra of intact and Ca2+
induced swollen mitochondria[25]
The results were observed that the structure of hemoglobin
changed due to the effect of direct current. When the current
increased, the content of hemoglobin decreased rapidly. Then,
this group[27] investigated the temperature effect on RBCs. It
was found that the RBCs cryopreserved could not be
conserved for more than 3 h after thawing in the solution, or
else the process of hemolytic occurs. The exposure of RBCs
to high concentration alcohol solutions may cause unwanted
biological effects. The denaturation process destroys the cell
in such a way that it will no longer be able to carry out its
cellular function. Deng et al[28] investigated the time evolution
of the Raman band at 752 cm?1 of single RBCs, which
corresponds to the porphyrin breathing mode. It was found
that the intensity of this band decreased by 34% over a period
of 25 min after the administration of alcohol. Also, it was
found that the decrease in the intensity of the band at 752
cm?1 became more rapid and more prominent as the alcohol
concentration increased. When the alcohol concentration
exceeded 30%, the RBCs broke down within 3 min and the
cell structure collapses. Therefore, such analytical potential of
Raman tweezers has been put to good use in real-time
detecting the stress responses at the single-cell level in
biomedicine. As such, it has the potential application in the
blood examination and the curative effect in the related
3.4 Metabolism of yeasts 3.4 Metabolism of yeasts
With technical development, Raman tweezers has a
capability of monitoring the phenomenon of life via studying
biological macromolecules. The changes of Raman spectra of
single yeast at the live and dead states are obvious[11]. When a
culture of yeast cells is inoculated in a fresh growth medium,
they enter a brief lag phase where they are biochemically
active but not divisional. The synthesis and degradation of
biological macromolecules are easily neglected by researchers
in this process. After this lag phase, cells enter their cell cycle
and start to divide. Messenger ribonucleic acid (mRNA) is
RNA that encodes and carries information from DNA during
transcription to the sites of protein synthesis to undergo
translation in order to yield a gene product. Singh et al[29]
found that the decrease observed in this RNA peak at 782
cm?1 in the ?rst 8 min after inoculation might thus be
attributed to either the biochemical degradation of mRNA or
a change in the conformation of ribosomes already present in
the cytoplasm or both. Otherwise, they found another
important result that during the initial lag phase, there was an
immediate pause or a shutdown in the synthesis of many
proteins that occurred just after inoculation. The levels of
protein present in the cells subsequently increased as the lag
phase progressed. The changes in the rate of the synthesis of
rRNA and ribosomal proteins during the lag phase enable the
cells to multiply at a rate adapted to the new environmental
conditions. As such, Raman tweezers has potential
capabilities to detect the law of life for example, consider
yeasts for a pattern at the single-cell level. However, this
technique is only fit to study the microbe with a short
life-cycle. For the microbe with a long life-cycle, the problem
of culturing circumstance in vitro and experimental condition
should be solved.
3.5 Germination of bacillus spores 3.5 Germination of bacillus spores
Ca2+-Dipicolinic Acid (Ca2+-DPA) is a composition of
Pyridine-2,6-dicarboxylic acid (dipicolinic acid [DPA]) in a
1:1 chelate with calcium ion. Ca2+-DPA is important for spore
resistance to many environmental stresses and spore stability.
In the germination of Bacillus thuringiensis spores, the
time-to-germination was found to be stochastic for individual
spores with a typical value of –30 min under the experimental
conditions. Within this time, the Ca2+-DPA content has two
states: the Ca2+-DPA content remains almost a constant in the
first period approximately from 0 to 29 min and then
decreases very rapidly due to its release into the medium
(within –2 min)[30]. Besides real-time detection, Raman
tweezers has the quantitative analytical capability of the
Ca2+-DPA level. In the Raman spectra of single bacillus
spores, Huang et al[16] found that Ca2+-DPA concentrations in
the spore core are > 800 mM, well above Ca-DPA solubility.
As such, in the dynamics process of the real-time detection of
the single germination of bacillus spores, Ca2+-DPA is taken
as biomarks to explore the mechanism of germination. Raman
tweezers is a technique to combine the noninvasive mark
technique with real-time detection of life, which is a great
progress of technical innovation. All these analytical
capabilities and progress would give the biologists a much
clear window to study life phenomena.
Page 5
AI Min et al. / Chinese Journal of Analytical Chemistry, 2009, 37(5): 758–763
3.6 Dynamics detection of proteins 3.6 Dynamics detection of proteins
Raman tweezers has high delicacy at the real-time,
noninvasive, quantitative detection of biological processes,
such as the express process of single protein[31,32]. Wang et al[33]
studied the Raman spectra of single parasporal crystal from
Bacillus thuringiensis using Raman tweezers. Chan et al[34]
used Raman tweezers to monitor the dynamics of the lipid
metabolism of individual triglyceride-rich lipoprotein
particles as they interacted with lipoprotein lipase in the
endothelial cell wall via Raman spectroscopy. However, these
studies only involved the dynamics processes between
proteins themselves. In the membrane channel, there still is a
vacuum in the detection of energy exchange in protein
transportation processes by means of Raman tweezers.
4 Advantages of Raman tweezers 4 Advantages of Raman tweezers
In this article, the part applications of Raman tweezers
were introduced to readers. In a word, besides high resolution,
delicacy, very short data collection, Raman tweezers has other
unique advantages as follows. First, it is a special technique to
optically fix single cells or organelles. Optical tweezers has
the capability to fix the cells or organelles nearly on the focus
of laser beam. Without fixation, cells or organelles will leave
the area of laser beam for exciting. However, other physical
or chemical method is usually used to fix the cells on the
glass in tradition, in order to keep cells not to leave the laser
beam. But this fixative technique cannot be used to act on the
cells in the solution and will change the microenvironment
around the cells, which will bring unwanted effects on them.
Second, it enhances the SNR of Raman signal from cells. The
scattering optical path is optimized due to trapping the cells
on the focus of laser beam, which can enhance SNR and
delicacy. Meanwhile, the fluorescence from the glass and the
stray light from optical path were decreased obviously due to
trapping the cells above the glass of about 10 ?m. Third, it is
an optical label technique and has the capability of the
real-time detection of biochemical processes. Instead of
fluorescence label, the characteristic peak is considered as
noninvasive label to real-time to detect single molecules of
cells and the physiological changes in the special time and
space. To sum up, Raman tweezers has many obvious
advantages of controlling single cells, noninvasive label,
detection of Raman signal, and it makes a new start at the
single-cell technique.
5 Prospects 5 Prospects
Raman tweezers has the unique capability that other
techniques cannot, especially in the analysis of single living
microparticles (0.5?10 ?m in diameter). However, many parts
of it require improvement for higher resolution and SNR,
better means to analyze Raman spectra. In order to make
optical tweezers trapping effectively and obtain high SNR,
some people used double-trap and confocal devices to
improve on it[35]. Double-trap is realized using two
spectroscopes in the optical path. One laser beam is divided
into two beams, which are then introduced into an inverted
microscope to form two tweezers. This double-trap has the
ability of acquiring the Raman signals of both cells and
background simultaneously. However, the signals of them are
weaker than that from single trap, respectively. These
relational experiments have been carried out in our laboratory
now. Double-trap is also realized by two different laser beams
from different laser setups, whose laser powers can be
adjusted, respectively. However, it is difficult to adjust the
focus of two traps on the same horizontal plane. The
settlement of these problems requires the improvements and
perfection of several theories and techniques in future.
Raman tweezers can exploit its potential application by
combining with other
microfluidics[16,24,31,36]. The study of Raman tweezers coupled
with a microfluidic device is on the beginning stage[37]. Some
people have successfully combined optical tweezers with
microfluidic chip[38], which has realized the simple
automation of a monitor by a computer. However, this
technique has not been combined with Raman spectroscopy.
Recently, the primary work has been carried out in our
laboratory. Based on the combination of Raman tweezers and
microfluidic chips[36], a computer controlled digital image
processing technique will be coupled with them and then
realize the automation of image acquisition and digital
processing, and finally realize the microfluid Raman-tweezers
biosensor system that includes Raman spectroscopy, optical
tweezers, and microfluidic device. In the near future, the
highly effective integration of multitechniques would have
been put into potential application in the studies of single-cell
detection, identification, sorting, and dynamics process,
especially in the physiological and biochemical researches,
high-throughput screening, diseases examination, medicine
diagnoses, pharmacological experiments, and the researches
of the law of life.
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[37] Jess P R T, Garcés-Chávez V, Smith D, Mazilu D, Paterson L,
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[38]Lin C C, Chen A, Lin C H. Biomed. Microdevices, 2008,
10(1): 55?63


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Guest Promise
East Burke, VT is a place carved from the natural abundance and shared values of its stalwart community.
It's a place built upon generations of preservation, pride, and unwavering integrity in an environment that's rooted in labor and love. And it is this spirit that is infused into every timber, nut, bolt, bed, and blanket you'll find in the Q Burke Hotel and Conference Center. From endless outdoor adventures to the exquisite indoor amenities within our Hotel, our goal is to provide you with an authentic experience uniquely your own, one which you can't wait to share or treasure yourself.
One of the greatest gifts the Northeast Kingdom offers to its residents and guests is the natural beauty and infinite opportunity for experience. Whether you like the outdoors or indoors, winter, summer, spring, or fall, the talented and familiar concierge staff at the Q Burke Hotel and Conference Hotel can point you in the right direction.
From skiing our 50 trails and glades, mountain biking over 100 miles of trails on and around Q Burke Mountain Resort, hiking, paddling, swimming, snowshoeing, cross country skiing, fat biking, and paragliding….well, we think you get the point. Your adventure starts here.
Hotel Services
- F&B: Fine-dining restaurant | Pub | Café
- Concierge
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Rates and packages
Early Season (December 11th through December 25th, 2015) starting from $173.00**
Holiday (December 26th – December 31st, 2015 ) starting from $403.00**
Regular Season (Jan 2nd through March 26th, 2016 | excluding Jan 15-17 and Feb 13-20) starting from $271.00**
Peak (Jan 15-17) & Presidents Week (Feb 13-20) starting from $353.00**
Late Season (March 27 through Apr 9) starting from $190.00**
**Ski & Stay packages for Studio room (2 adults) per night. 1-bedroom, 2-bedroom, 3-bedroom unit types also available. Click here for more information on our Hotel Room Options,
Early / Late Summer (Apr 10th through July 1st | Sept. 5th through Dec 9th, 2016) starting from $134.00**
Peak Summer (July 2nd through Sept. 4th, 2016) starting from $166.00**
**Bike & Stay packages for Studio room (2 adults) per night. 1-bedroom, 2-bedroom, 3-bedroom unit types also available as well as lodging only packages. Click here for more information on our Hotel Room Options.
Projected opening date is December 11th, 2015, now accepting Reservations.
The front of the hotel.
The view from the mountain.


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Andrew Smith witnessed a lesson in evasion, denial and fabrication.
Filed in: Arms Corporations England Trade
Page 2 of 10
Andrew Smith witnessed a lesson in evasion, denial and fabrication.
Filed in: Arms Corporations England Trade
Campaigners' blind defence of the government's development strategy is making things worse, says Nick Dearden.
Filed in: Development (Aid) United Kingdom
Where have the traditions of intellectual freedom gone? ask Benoit Dutilleul and Holly-Rae Smith.
Eastern European migrants in Britain are fielding candidates to keep out the xenophobes, reports Ylenia Gostoli.
Slovakia isolates this persecuted population from the rest of society by building walls and segregating schools, says Lydia James.
Filed in: Minorities Slovakia
Hanneke Hagenaars, a transplant co-ordinator for deceased organ donors in the Netherlands, speaks about her liaison work with soon-to-be-bereaved families.
Filed in: Health Netherlands Society
A government that favours the rich and shuns the poor could not be further away from the Bible's vision of an equal and radical society, says Symon Hill.
Filed in: Christianity United Kingdom
When will the government take action to protect migrant domestic workers? asks Tony Mckenna.
The rise of the 'hidden homeless' is being ignored by the country's government. Cinzia D'Ambrosi has captured lives in limbo.
Filed in: Housing Poverty United Kingdom
Jacob Wills laments landlords and local councils' willingness to evict the capital's tenants. Could it be because a third of MPs are landlords?
Filed in: Housing United Kingdom
Support community initiatives by walking from Devon to Dorset, says Jonny Gordon-Farleigh.
Filed in: Social Change United Kingdom
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Happy Birthday. by Afrosoldier303Watch
©2014-2015 Afrosoldier303
A bit of a concept for Jordy Before he was damaged. Fresh out of the box and fully assembled.
This was actually an idea I had for a larger project I'm doing for my school. Our principal wants more art to be
hung up around the school and I was among the few who volunteered.
This'l be an interesting experience indeed because I'm gonna have to paint this on a much larger canvas.


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- Jessa Duggar shares a rare photo of her brother Josh Duggar's daughter Meredith - US Weekly
- Michael Lohan's estranged wife Kate Major is detained by cops at an airport - TMZ
- Is there something between Ben Affleck and Halle Berry? - Gossip Cop
- The Hunger Games author Suzanne Collins says goodbye to the popular series after the final movie is released - Just Jared Jr
- What happened backstage with Kylie Jenner at The Ellen Show? - Radar
- Bryan Cranston tears up over a Breaking Bad memory - Huffington Post
- Leonardo DiCaprio's Oscar race heats up - Lainey Gossip
- The Captain America: Civil War movie may be better than the comic book - The Hollywood Reporter
11 December 2013 at 12:01 PM
Kim Kardashian: THR's Women in Entertainment Breakfast!
About this photo set: Kim Kardashian is a golden gal while attending the 2013 Hollywood Reporter Women in Entertainment Breakfast held at the Beverly Hills Hotel on Wednesday morning… Read More Here
Photo #: 3009788
Posted to Kim Kardashian


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Tirtha Luhur WeddingHere at SMP, we're total suckers for a good love story. So when I heard about these two sweeties whose paths fatefully crossed on the isle of Greece 7 YEARS after their first meeting, I nearly went weak at the knees. It'sRead More On SMP


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Ms. Couch shouldn't look for bait so hard! I'm well aware of the injustices she cites. I merely said that addressing them did not...
Thanks, Tybeenian! I think what this country needs is a religious revival. Electing any specific politicians instead of any other...
God Bless!
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I have found that people who "preach" logic and science over God are basically just enamored of their own intellects. If...


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Kabuki Plays on Stage: Darkness and desire, 1804-1864
James R. Brandon, Samuel L. Leiter
University of Hawaiʻi Press, Jan 1, 2002 - Drama - 416 pages
Darkness and Desire, 1804-1864, is the third volume in a monumental new series - the first collection of kabuki play translations to be published in nearly a quarter of a century. Fifty-one plays, published in four volumes, vividly trace kabuki's changing relations to Japanese society during the premodern era. The fourteen plays translated in Volume 3, Darkness and Desire, 1804-1864, mark an extreme point in the development of kabuki dramaturgy. The plays are remarkable, even within kabuki, for their intense theatricality, gutsy individualism of character, cold-blooded and ferocious violence, realism pushed into fantasy and grotesquery, novelty for its own sake, sexual aggressiveness, and assertion of female will. The plays depict a society in extremis, the end of an era, a time often marked by unmitigated darkness and desire.
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actor Akamaya audience bell bows BUNYA characters CHOBEI clack curtain dance Danjuro DOEMON dorodoro double tsuke beats face father flute front GENJI GENZAEMON ghost plays GONPACHI GOROZO hanamichi hand head HEINAI HENCHMAN holds hundred gold pieces Ichikawa Ichimura-za IEMON JINBEI JUZABURO kabuki Kabuki-za Kamigata KASANE KIHEI Kikugoro kimono KISEN KISUKE KIYOMOTO KOMACHI KUSUKE kyogen KYUSAKU large drum looks Lord Masakado mimes MITSUKUNI Mokuami NAGAUTA Nakamura-za Nanboku NAOSUKE offstage shamisen OIWA OROKU OSAN OSHO OSHU Osome OSOME AND HISAMATSU OTOMI OTOSE OYUMI palanquin pattern performed poses ROBBER role samurai Sanbaso SATSUKI Scarface Scarface Otomi scene shamisen accompaniment shichisan shoulder sits Six Poet sleeve SOGO SOKAN song STAGE ASSISTANT stands stick drum stops Stories at Yotsuya Suzugamori sword TAKEMOTO TAKIYASHA temple Three Kichisas TOKUBEI translated Tsuruya Nanboku IV Waseda University wears wisteria YASU Yasuna YOSABURO ZENROKU


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Sue May specializes in conducting searches for leadership, academic, and managerial positions within higher education, with additional experience finding leaders for select non-profit institutions, think tanks, and independent schools.
Before joining Storbeck/Pimentel, Sue worked for higher education strategy consulting firm Art & Science Group, LLC. She managed year-long client engagements with more than 25 institutions across the country, ranging from small women's colleges to large research universities. She regularly presented to faculty bodies and governing boards, and provided confidential counsel to college and university leadership.
An expert on alternative careers for graduate students, the third edition of Sue's book -- So What Are You Going to Do With That?: Finding Careers Outside Academia– was published by University of Chicago Press in 2015. Sue has published 18+ articles in the Chronicle of Higher Education's "Beyond the Ivory Tower" column and given more than 35 invited lectures at universities around the country.
Storbeck / Pimentel & Associates, LP
Baltimore, MD
Phone: 410/243-1012
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The first Thanksgiving was a celebration of the Pilgrim's bountiful 1621 harvest and a thank you to the Wampanoag tribe, members of which had taught the starving zealots to plant New World-appropriate crops and catch exotic species of fish. One good turn deserved another.
Unfortunately, most meetings of so-called modern communities don't lead to bread breaking, much less national holidays. Not to rain on the Thanksgiving parade, but New England's tribes found themselves at war with invaders little more than a decade after the great feast. Indigenous communities have been pushed to the edges of the map by prospectors and political agendas.
They aren't gone.
The world may be flat and globalization may be an inexorable trend, but tribes stills persist all over the world. Finding them may not be easy, but these native peoples have managed to maintain traditions while living in the forests they've called home for as long as they can remember.
Survival International's Joanna Eede shared the following tour of the shrinking world of modern, indigenous peoples. SI has just publishedTribal People For Tomorrow's World, Stephen Corry's survey of the issues affecting native populations the world over.
'We love the forest as we love our own bodies,' say the 'Pygmy' peoples who live in the densely forested regions of Central and West Africa. Each group -- the Twa, Aka, Ba'Aka and Mbuti -- is a distinct people with diverse languages, yet one word is common to all: jengi, which means the spirit of the forest. Pygmy men will scale immense trees in search of honey, and are such proficient mimics they can imitate the sound of a distressed antelope in order to lure another out of the bush. Picture © Kate Eshelby
In recent years the pygmies' homelands have been devastated by logging, war and encroachment by farmers. Many conservation schemes that aim to establish wilderness reserves deny the land rights of tribal peoples, who are evicted to the margins of their homelands; the Batwa pygmies were forcibly moved from Uganda's Bwindi Forest in order to protect the mountain gorillas. This variant of land theft is rapidly emerging as one of the biggest problems confronting indigenous peoples today, says Stephen Corry of Survival International All my ancestors have lived on these lands, said a Batwa man. As a result of the eviction, everybody is now scattered. Picture © Kate Eshelby
Penan hunters in the ancient rainforest of Sarawak in Borneo -- one of the most biologically rich forests on earth. The Penan have long lived in harmony with their forest and its vast trees, rare orchids and fast-flowing rivers. Until the 1960s all Penan people lived as nomads, moving camp frequently in search of boar, and following the cycles of fruiting trees and wild sago palm. Today, most of the 10-12,000 Penan have settled in riverside communities, although some are still largely nomadic. The land is sacred, they say. It belongs to the countless numbers who are dead, those who are living and the multitudes yet to be born.
The Penan call the forest okoo bu'un; the place of their origins. Since the 1970s, the Penan's rainforests have been cleared for logging, oil-palm plantations, gas pipelines and hydroelectric dams, says Sophie Grig of Survival International The steep-sided valleys, that once were filled with birdsong, now resound with the noise of trucks and falling trees. Dusty red logging roads transport bulldozers deep into the forest. It is hard for us to look at the red land, say the Penan.
To conquistadores, colonists and corporations, the Amazon -- the largest rainforest in the world -- has always signified power and profit. To 1 million Indians, it is simply home. We Indians were born here, we live, work and we will die here, said an Harakmbut Indian from Peru. Picture © Hutukara/ISA
Deep in one of the remotest parts of the Brazilian Amazon, uncontacted Indians look up at an aeroplane. There are over 100 uncontacted tribes worldwide: peoples with no peaceful contact with anyone else. We know very little about them. But we do know that they want to be left alone. That is their choice - and their right. Picture © Gleison Miranda / FUNAI
We know our forest land well, says Davi Kopenawa. As they would: the Yanomami people have lived in it for thousands of years. Their botanical knowledge is extraordinary. Babies' slings are made from silk-grass twine, arrow shafts from the stems of pampas grass and salt is extracted from the ashes of the great Taurari tree. The Yanomami think and speak with the soul of the forest, says Davi. Picture © Fiona Watson
In recent decades, the Yanomami have suffered greatly. In the 1980s, over 1,000 goldminers invaded their territory. As a result, nearly a fifth of the population died from measles and other illnesses to which they had no immunity. The campaign led by Survival International resulted in the creation of the Yanomami Park in 1992. Threats are ever-present, however. Gold-miners are still working illegally in the forest, alongside cattle ranchers who are deforesting the eastern fringe of their land, says Fiona Watson of Survival. You can't uproot us and put us in another land, says Davi Kopenawa.We don't exist away from the forest. We belong in it. Picture © Survival International
In the heart of the southern Brazilian state of Mato Grosso do Sul, Enawene Nawe children throw themselves into a tannin-coloured river. The Enawene Nawe are expert fishermen; the men spend up to four months living deep in the forest, smoking fish caught with intricate wooden dams and sending them back to villages by canoe. All this land belongs to the yakairiti, who are the owners of the natural resources, they say. Picture © Fiona Watson
It was beautiful here, says an Enawene Nawe man. Telegráfica Dam is one the many dams under construction on the River Juruena. It is killing the fish the Enawene Nawe rely on. The tribe were not consulted about the project. If you finish off the earth and the fish, the yakairiti will take vengeance and kill the Enawene Nawe, says an Enawene Nawe man. Picture © Survival International
A Guarani man stands at the side of a dusty road, his arms outstretched, a mbaraka rattle in his left hand. The deforestation of Mato Grosso do Sul has forced many Guarani -- the original owners of the forest -- onto tiny patches of land. Gone, for most, are the gardens where they planted manioc and corn; gone is the ability to hunt game freely. Instead, they are surrounded by cattle ranches, soya fields, and sugar cane plantations. The impact of this land loss on the Guarani people has been profound. You become spiritually empty,said one man. Picture © Sarah Shenker/Survival
The tribal peoples of the world's rainforests believe their homes demand greater respect. Yet still the forests are slashed, felled and mined. As the trees fall and the smoke rises, so the indigenous communities are illegally evicted from their homes. One of the simplest ways of preserving the world's rainforests is to secure the rights of tribal peoples who live in them. We, the indigenous peoples, have not forgotten that man is part of nature, says Davi Kopenawa. If we hurt nature, we also hurt ourselves. We know how to protect the forests. Give them back to us, before they die. Picture © Kate Eshelby
The trees hold a very special meaning and purpose to all living things. In return, they must be treated with kindness and respect. Will their kindness to us be forgotten? Will the respect they are due be ignored? Will the truth they represent be, quite literally, cut down? Mike Koostachin, Cree, Canada. Picture © Thomas Quirynen
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This lovely vintage Fenton vase is in the Flute and Dot pattern in cranberry.
The vase measures 5 1/8 inches high and approximately 4 inches in diameter at the top.
This hand blown vase has a smoothly ground pontil mark on the bottom.
This Fenton vase is in excellent condition with no chips, cracks. There are two construction issues: a tiny included bubble and a cooling mark (see smaller pictures for close-ups).
The charming piece of Fenton glass would be an outstanding addition to your Fenton or antique or vintage glass collection. Makes a wonderful gift.
WELCOME!! We have that special antique or vintage collectible to suit every need and taste.
Antiques & Vintage Collectibles are ideal gifts for Birthdays Anniversaries Weddings Graduations WHY? See our Home page!


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In liberal land, also known as Los Angeles, it should be no shock that the City Council wants to repeal the First Amendment. After all, they are a bunch of liberal hacks and they cannot stand the idea that someone disagrees with them. The LA city council would do so well in the kind of socialist dictatorship they seem to prefer.
What has happened now?
From Los Angeles CBS:
City Council members took a step closer on Wednesday to becoming the first in the nation to adopt a resolution condemning certain types of speech on public airwaves.
Councilmember Jan Perry introduced legislation that would call upon media companies to ensure "on-air hosts do not use and promote racist and sexist slurs" on radio and other broadcasts.
Members of Black Media Alliance, National Hispanic Media Coalition, Korean-American Bar Association, American Indians in Film and Television were on hand to voice their support for the proposal.
The resolution — which was also supported by Councilmember Bernard Parks and Council President Herb Wesson — called attention to the recent uproar over comments by KFI 640 AM talk show hosts John Kobylt and Ken Chiampou.
Kobylt and Chiampou were suspended after they called the late pop singer Whitney Houston a "crack ho" three days after her death in February.
The proposal cites a "long history of racially offensive comments as well as deplorable sexist remarks, particularly towards women and Black, Latino, and Asian communities" at KFI 640 and calls for parent company Clear Channel Communications to hire a more diverse workforce to offset the trend.
I have one brief message for the Los Angeles city council and my message is protected by the First Amendment.
The Whitney Houston incident is simply a pretext. They do not want diversity. In fact, they want just the opposite. They want conservatives silenced.
The Los Angeles city council's idea of deplorable comments is conservative speech. They do not like people pointing out that socialism sucks.
I have a better idea. Instead of a resolution repealing the First Amendment, why don't you clowns on the LA city council resign? You are not public servants. You are a disgrace. You are a disgrace to America.
Since one third of LA students drop out, perhaps you Bozos could join them in a remedial civics class that would start with an explanation of the First Amendment and freedom of speech.
In the meanwhile, hopefully Clear Channel will tell the LA city council to go to hell and how to get there.
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But it is still OK to hurt the white male right? I'll bet it is. No one will care what happens to the white man as long as no blacks or any other race and women don't get hurt. Riiight. SCREW YOU is right Judson. I am so sick of this B.S. Really I am. I refuse to bow to anyone! The only ones I let dictate my life were Mom and Dad..I am glad that I did. Out side of that..NO ONE TELLS ME WHAT TO DO!
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