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Rimmel 60 Seconds Nail Polish
302 reviews
81% would repurchase
Package Quality: 3.9
Price: $$
Package Quality: 3.9
Price: $$
Age: 19-24
Skin: Combination, Dark, Warm
Hair: Brown, Other, Other
Eyes: Brown
I have this in green with envy. Can't remember the exact price I got it for but it was a buy 2 get 1 free at superdrug. The pigmentation is amazing and it lasts a really long time and I do dishes every now and then. U don't even need a base coat or top coat with this and it doesn't stain nails too when u take it off which is a huge plus from me.
Age: 19-24
Skin: Combination, Fair-Medium, Cool
Hair: Brunette, Curly, Medium
Eyes: Brown
I got mine on clearance at cvs for 75% off so I ended up paying 1.25 great price for a great polish. It literally dries in 60 seconds or even less and lasts for roughly about a week. I'm excited that it dries so quickly because I'm extremely clumsy and usually tend to mess up my polish before it dries this formula doesn't give me a chance to do that.
Age: 25-29
Skin: Acne-prone, Tan, Not Sure
Hair: Black, Other, Other
Eyes: Brown
Pretty good polish, it dries as fast as it says it does and the application is fast too. It doesn't chip if you put on a top coat (even a cheapie low-end top coat) but will chip if you don't. It is a little streaky but that can be disguised with another coat (I'd only go 2 coats). Pretty good for the price and good colours too!
Age: 18 & Under
Skin: Combination, Fair-Medium, Not Sure
Hair: Brunette, Other, Other
Eyes: Blue
I have one colour of this nailpolish, grey. It's a simple but a real nice colour. The product is �7,99 and I think that's a bit expensive, but if you compare that with the quality, wauw. It stayed around 4 days without topcoat on my nails. No chipping. I have mostly Catrice nailpolish and that stays like 1/2 days? It's a lot cheaper but I would chose for Rimmel.
Age: 25-29
Skin: Dry, Fair, Not Sure
Hair: Red, Straight, Medium
Eyes: Green
Love this polish! Great thickness, goes on so easily with the big brush, and dries fast. I rarely "colour outside the lines" with this brush! Also lasts really well too. Pity they don't make all Rimmel polishes in this formula. My favourite colour is Grey Matter.
Age: 18 & Under
Skin: Oily, Olive, Not Sure
Hair: Brown, Other, Coarse
Eyes: Black
I would definately buy this product again. The colours are great and the shimmer ones look really good on my nails. It does dry very quickly but it does take more than one stroke to get the nail polish on. I always put two coats on so it gives my nails the full colour and not just a translucent look. But it is a really good buy and its cheap at around $8
Age: 19-24
Skin: Combination, Olive, Not Sure
Hair: Brunette, Other, Other
Eyes: Brown
I don't think Rimmel colours are that great. They don't offer a huge variety and there are no 'stand out' colours which offer shades other brands don't.
The bottles are usually rather small for the price you pay and they don't last very long. You normally have to apply two coats to get an even colour.
I don't think quality is that great either. Nearly every Rimmel polish I have bought dry up or get clumpy in the bottles very quickly. I constantly have to thin them out myself a few weeks after buying the polish.
I hardly ever buy from this brand anymore as I think other brands offer much better nail products.
Age: 18 & Under
Skin: Acne-prone, Fair, Not Sure
Hair: Brown, Other, Other
Eyes: Blue
have got this is Shine on the silver, dries quick but yeah that can be a pain cuz you cant rreally spread it right. so i end up going over it again and agian and it gets really thick. otherwise, lasts like a whole week but i dont wash dishes or anything and only shower like every other day
Age: 18 & Under
Skin: Dry, Medium, Not Sure
Hair: Brunette, Other, Other
Eyes: Green
Good. I have it in red carpet. The red is perfect. The downside is this
Owned 2 days: dries in 40 secs
Owned 2 months: dries in 2 mins
Other than that great.
Age: 18 & Under
Skin: Combination, Fair, Not Sure
Hair: Brunette, Other, Other
Eyes: Brown
It dries fast, like it says on the bottle, and the colour is good, very opaque, so one coat really does the job:)
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Marc Jacobs Lust for Lacquer Lip Vinyl in 'Overprotected' + Barry M Gelly Nail Paint in 'Huckleberry'
Have you got any summery combination you think I should try?
- Elodie x
Love all of these combinations!
Ah I do love bright nails! Great picks Elodie!
Love the first combinations! If only glitter polishes were easier to remove!ReplyDelete
Those YSL lippies look gorgeous!ReplyDelete
Lovely combinations for charming and gorgeous look. Keep sharing!ReplyDelete
Thanks for commenting. I love hearing from you!
If you have any question, feel free to tweet me at @ElleYeah and I'll make sure to get back to you as soon as possible.
-Elodie x

subset: commoncrawl


text: Leno, Fallon Duet on Tonight's Future Hosts show their friendship, West Side Story -style By Matt Cantor, Newser User Posted Apr 2, 2013 7:18 AM CDT STORY COMMENTS (4) (Newser) – Jay Leno and Jimmy Fallon have had it up to here with hearsay about the Tonight Show's future, and they're doing something about it: singing. The two hosts launched into a version of "Tonight, Tonight" from West Side Story last night, reflecting on what's next for the show, Entertainment Tonight reports. "Tonight, tonight. Why do they say we fight? I like you. You like me. We're OK," they sang. "Tonight, tonight, who cares who hosts Tonight? People just watch online the next day." Leno, Fallon Duet on Tonight's Future Back to story » Jimmy and Jay sing. (YouTube) Popular Video Stories Entertainment Tonight (Source Grid ») Entertainment • Celebrity • Media entertainment • NBC • humor • Jay Leno • late night talk show • late night host • song • Tonight Show • Jimmy Fallon CLICK BELOW TO VOTE 70% 4% 0% 15% 0% 11% RETURN TO THE STORY To report an error on this story, notify our editors.


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Browse the comprehensive database of Beaver City car dealers online at Autobytel.com. Look for your future new or used automobile by manufacturer, value, type, or production year. Autobytel has a huge directory of Beaver City car dealerships with their inventories of new and used car, truck, and SUV listings online. We will help with your automobile shopping experience every step of the way. View up-to-date values of automobiles, study vehicle stats, browse photos, research extensive analysis of the cars, and find prices pertaining to the Beaver City car dealership.
Times have changed and the need for visiting Beaver City car dealerships has been rendered obsolete. Thanks to the world wide web; Beaver City car dealers have the choice of having all of their stock of vehicles listed at various places like Autobytel.com. And the majority of car shoppers will begin to research automobiles online. Most car buyers will seek out the inventories of Beaver City car dealers once they have figured out which automobile is best for them. Odds are they will find themselves located at a viable online automotive resource like Autobytel.com. Get a price quote from multiple Beaver City car dealers online at our website.
Putting faith in one Beaver City car dealership can represent a huge step. Do not presume that the only trip you will make to the Beaver City car dealer is to close the deal on a new or used car. There is a good chance that since Beaver City car dealers offer much more than new or used car sales; you will do business there again. Proper Beaver City car dealerships will have more than a car sales division; they will also have on the premises: a parts and accessories department, a service and repair center, a collision shop, and a finance and lease department. The possibility is very high that you will again go back to the Beaver City car dealer establishment if you ever need anything for your vehicle. That's why it is a good idea to form a close bond with the Beaver City car dealership in the event that you need to use one of their multiple services.
The Beaver City car dealerships directory at Autobytel.com also features some great information in the form of reviews and ratings. Peruse accounts given by shoppers of Beaver City car dealers to find out valuable details. This could be an effective tool at narrowing down which Beaver City car dealerships can most closely fit your new or used car buying needs.


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Not sure if this had been posted before, but after reading this I could not help but laugh out loud. A couple gems:
"I got the most out of my opportunities. Let's face it – they didn't throw the ball to me as much as they should have. You can look at the tapes and see how open I was. Should I have had more catches? Absolutely."
"I did the best with what I was given. The two years of losing? Miserable. Steve (Young) never threw me the ball enough. Jeff (Garcia) never threw me the ball enough. They could have thrown more balls my way."
I started thing about JJ today, because of Crabtree. I remember what a shock it was when we traded up to NUMBER 10 to grab him. I distinctly remember Berman saying "And the rich get richer" referring of course to the defending super bowl champs getting a supposed play-maker. I do not for one second believe that Crabtree will have a similar underachieving career like Stokes, but I will say that the excitement is somewhat remniscent to draft day 14 years ago.
But way to go J.J., I'm sure sports talk radio in Modesto is every Bruin's dream.


text:
"West Hartford" West Hartford by johncudw
West Hartford Travel Guide: 7 reviews and 22 photos
This bridge is off Waterside Lane
View from Tunxis Road - early summer evening
Nice place for photos of the view of this small lake
This is the view east from Lower Rumford Street
Hartford skyline is visible on the horizon - CityPlace
This is the neighborhood that I grew up in and is 1.5 miles south
of West Hartford Center. A residential section of town
about 1 mile long and 1/2 mile wide.
Dogwood tree in bloom
This is a beautiful park with flowering trees and a rose garden
There are tulips and flowering trees in April and roses in June.
Off Prospect Avenue near the Hartford border
This is my former home town where I grew up and went to
high school
- Pros:short commute to Hartford, nice residential town
- Cons:not many open spaces left
Reviews (4)
Beautiful extensive tulip garden over on the west of the rose garden. Doesn't last too long, This was May 3, 2007. Nice... more travel advice
This town is fairly flat and has neighborhoods that are good for bicycle riding. This is me with my new Motobecane... more travel advice
There are roads, paved, and dirt for bicycles and mountain bikes. Some of the dirt roads and all trails, are off limits... more travel advice
In mid-June is when most of the roses come out. Off Prospect Avenue - the border between West Hartford and Hartford. A... more travel advice
Comments (1)
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text:
Credit unions offer voter registration at branches
Credit unions may be a convenient place to obtain a loan, open a savings account or, now, register to vote.
Utah credit unions launched a 30-day campaign Wednesday at the Capitol seeking to make it easier for the state's estimated 750,000 unregistered adults to get on Utah voter rolls before next month's political-party caucuses, the first step to nominating candidates.
"We're trying to make that as easy as possible," by allowing registration at credit-union branches or finding links online at utahscreditunions.org, said Scott Simpson, president of the Utah Credit Union Association.
He said many may ask why the association is doing the campaign now instead of nearer to the November general election. He said the election in many cases would be over long before then with March caucuses, April conventions and June primaries choosing real winners in heavily Republican Utah.
Simpson said when he was the executive director of the state GOP, it never spent a dime to contact anyone who was not a registered voter. "If you are not registered, you really are not part of the dialogue."
Credit unions had about 100 of their members who had just registered at their announcement, in front of decorations depicting the beginning of a race with the theme: Ready, Set, Vote.
"We're walking the walk by registering our employees and members to vote and encourage the public to join us," he said.


text:
"When man first set woman on two wheels with a pair of pedals, did he know, I wonder, that he had rent the veil of the harem in twain? A woman on a bicycle has all the world before her where to choose; she can go where she will, no man hindering." The Typewriter Girl, 1899.
"Every so often a bird gets up and flies some place it's drawn to. I don't suppose it could tell you why, but it does it anyway." Ian Hibell, 1934-2008
Looks like a trap. Notice how much those pods look like those in The Matrix?
Pivot 5.7
Rawland Drakkar 650B love it with 38mm Soma tires
2009 Specialized Roubaix Ultegra
Santana Fusion Tandem
2010 Specialized TriCross
Challenge Concept Trike
One knee scoped so far.
Homophobia is so gay.
Give a maglev frictionless coupling, and I'm in.
My Book: Drive, Ride, Repeat: The Mostly-True Account of a Cross-Country Car and Bicycle Adventure
".... let the faster people "push" the slow rider in front... so the front one is taking the wind resistance but all the ones behind her are basically extra engines to counter that wind resistance. They'll act as a team. Everyone will just go up to a higher gear, and they'll all pedal together and all their energies will be combined."
Hello everybody! I'm the new girl here! Schweeb is the only way I could ever be on a team! I like the thought of that : ) I'll take the front and pedal as hard as I can for as long as I can, and when I'm beat, I'll rest while the "extra engines" keep going. Then it's my famous apple pie for the team-- its special because I make it with brown sugar *
Wish they'd stick to "Bike Lanes" mainly, the "Bike Paths" around here are pretty but they reall DO NOT get you to any useful destination! I end up taking the "street or road" to get to where I need to go, maybe that's just me being a "Utility/Rec./Touring" Rider, I need to get some place in a certain amount of time. LOL, pls. don 't say take a City Bus, here in Colorado Springs, the Bus is worse than the "Bike Path's" for getting anywhere in a decent amount of time, oh and NO buses after 6 PM, Arrrrugh!
Although I really like the idea of being "pushed" by others, LOL!
Take care, RIDE SAFE, have FUN!
B.J. Ondo
1993 Mongoose Switchback Ridged MTB, converted to a "Rail Trail Bike"! :)
I saw this in a thread in Road Cycling sub-forum this morning:
The article that the OP posted is here.
And the YouTube...
Wow you old guys sure are grumpy and closed minded.
Thankfully one day you'll all be dead leaving the rest of us to remake the world as we see fit.
The dumbest cycling ideas ever? These gems all come from cyclists:
"Bonking is caused by not using clipless pedals on a long ride." (How important is cleat placement for blood sugar levels?)
"Bicycles don't harm the earth." (Yep, having that Taiwan made frame shipped to your shop didn't cost a drop of fossil fuel.)
"Most bike tourists are female and slender." (Perhaps if you never tour outside the Isle of Lesbos.)
But, you can trust me. I'm not like the others.
RANS V3, RANS V-Rex, RANS Screamer - (cromo all)
Wouldn't work well in this corner of the 'verse".
2008 Kona Fire Mountain/Xtracycle
I void warranties.
Cycling well IS Cycling Advocacy
Originally Posted by Steely Dan: if you're riding a bike and not having fun, then you're doing it wrong.
I think this is awesome. Given the combination of low wind resistance and roll resistance and the fact that you could bypass stations these could nearly compete with rapid transit for speed. In certain city corridors the cost and speed of this could make it a very attractive option.
Obviously there are practical issues to overcome, everything has practical issues to overcome.
Why such vehement opposition though? Just because it's new and different it's inherently bad? It's a wonder we ever got the wheel rolling.
Throw in a little sweat,body oder and a few farts and I am sure every one would want to get in one.
^^^Not to mention the homeless guys sleeping in them getting pushed around by all the busy workers.
never even considered the pathogen factor, that's why I hate the the streetcar in the winter... everyone refuses to open a window to create airflow and then sits there coughing and sniffling.


text:
A Living Heritage of Faith: The Bondarenko Icon Collection-Rediscovered Treasures
Click for Enlarged View
Item Number:
50 pages
Fondiazione Russia Cristiana
Copyright date:
This product usually ships on the next business day.
A soft cover, spiral bound book with protective over cover, measuring 12 x 13.5 inches and containing 24 full-page color plates. This book was originally produced as a calendar, and this printing and binding remove the calendar portions and deliver a durable book on icons to be treasured. The subject is the icons comprising the Bondarenko collection, spanning the fifteenth to twentieth centuries, and published herein for the first time. The accompanying text by Irina Salina describes the Bondarenko collection, its formation and the breadth of its icons, centered on very old icons from Novgorod, the North and Central Russia. The format of this book is to provide indepth treatment of the selected 24 icons from the collection presented in color plates. Thus, each icon has its own essay treating history and provenance, subject matter and imagery, etc. Subject icons include: Georgian Mother of God Hodigitria (c. 1530); Saviour Pantokrator with view of Kremlin (early 18th C.); St. Nicholas of Zarajsk (late 15th C.); Mother of God of the Caves (late 18th C.); Entry into Jerusalem (c. 1430); Christ the Savior (early 15th C.) Nikitin?s Trinity of the Old Testament (c. 1690); Mother of God of Kazan (c. 1720).
Benjamin Williams
While this book is akin to museum?s exhibit book for sale during exhibitions, it is also different in that it focuses on 24 exquisite examples, and doesn?t overwhelm with reams of detail. The quality of the book printing and binding is top notch, and the price reflects high quality paper and the printing of full color plates. Admittedly, it is focused on icons in the Bondarenko collection, but the treatment afforded each ion spanning the 15th to 20th centuries provides an in-depth understanding of the development of Russian iconography, as well as glorious images to be savored.
Copyright © 2000-


text:
Most actors are lucky to have one iconic TV role in their lifetimes. Andy Griffith, who died on Tuesday (July 3) at age 86, had two, in addition to a big screen career that included one of legendary director Elia Kazan's powerful, prophetic cautionary tales about the power of TV, "A Face in the Crowd."
WITN News reported that paramedics were called to Griffith's Dare County, North Carolina, home on Tuesday, where they found the actor dead.
For photos of Andy Griffith through his life, click here.
Griffith is best known for his role as the genial sheriff of the fictional town of Mayberry on the long-running "The Andy Griffith Show," which not only made Griffith a star, but also helped launch the career of actor/director Ron Howard, who played the sheriff's son, Opie. The show ran from 1960-1968 and though its iconic whistling theme song and his character became a part of TV history, Griffith was never nominated for an Emmy during his run.
Born Andrew Samuel Griffith on June 1, 1926, in Mount Airy, North Carolina, Griffith was an only child who cultivated a love of acting from an early age, graduating with a degree in music from the University of North Carolina Chapel Hill in 1949. He went on to star on Broadway and graduated to the movies in 1957 with Kazan's "A Face in the Crowd."
The movie tells the tale of the folksy drifter Larry "Lonesome" Rhodes who is discovered by a talent scout and lifted to fame in a national TV show and a role as a media coach for an ambitious presidential candidate. The rise-and-fall of Rhodes, whose arrogance and cynicism about his audience eventually get the best of him, is a still relevant tale of the wages, and costs, of instant fame, the power of TV and the capacity for Americans to forgive and forget the trespasses of their Hollywood heroes.
The "Griffith Show" was spawned from a 1960 appearance on the Danny Thomas show "Make Room for Daddy" and turned into the role of a lifetime for the actor. Playing widower Andy Taylor, Griffith radiated a wholesome good-heartedness that endeared him to the audience and helped make the show a hit. It also featured rubber-faced character actor Don Knotts in the role of bumbling deputy and Andy's best friend, Barney Fife. While Griffith never scored Emmy gold, Knotts won five straight Emmys for his work.
Griffith left the show in 1968 over a contract dispute and went on to star in a series of lesser-known series throughout the 1970s. He hit the jackpot again, though, in 1986 with the role of genial, sometimes-cranky country criminal defense attorney Benjamin Matlock. The legal drama ran on NBC from 1986-1992 and on ABC from 1992-1995.
The Presidential Medal of Freedom honoree, who showed off his pipes in "Crowd," "Matlock" and "Griffith," was also a recording artist, releasing more than a dozen albums of gospel hymns and country and bluegrass tunes.


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Find out what you can buy with a Take it away loan
Find out how much you can borrow with Take it away
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Take it away offers interest-free loans for the purchase of musical instruments
There are still tickets for @musicforyouth #proms tonight and tomorrow!
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24 Nov
@JazzUK will hold its first ever #4Jazz Festival in Coventry on the 27-28 November, make sure you check them out
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Couric / Palin Open
Couric / Palin Open
The Olympia Restaurant
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Sarah Palin already has a place in history. But her legacy also overlaps with that of Tina Fey, who did a dead-on impression of the Alaska governor on "Saturday Night Live" during the 2008 election season. When the Palin pick was announced, executive producer Lorne Michaels recalled to Entertainment Weekly, "the audience cast Tina. You'd read or people would come up to you and say, what a gift." Never mind that Fey had left the show to write and star in the sitcom "30 Rock" and a current cast member was available. "I think if we had used Kristin [Wiig], who I think would've done a brilliant Sarah Palin, the audience would've been disappointed. No question about it," Michaels added. When Fey donned the trademark Palin specs and hairstyle, the likeness was so uncanny that Fox News once accidentally ran a photo of Fey instead of Palin during a news story about the politician. Palin eventually came on the show, but Fey did not want to share the screen with her, worried that her appearance with the candidate would come off as an endorsement, Fey recounted in her book, "Bossypants." So Palin appeared with Lorne Michaels backstage and not with Fey. Even though the two kept their distance, the joint appearance resulted in the highest ratings since the 1994 show hosted by Olympian Nancy Kerrigan. According to Fey's account in "Bossypants," Palin reached out to Fey, even offering her daughter Bristol's babysitting services during the show for Fey's child. Fey declined the offer — her daughter was too young to stay for the show at the time. Still, Fey wrote, "I appreciated the mom-ness of Mrs. Palin's offer." In this 2008 clip, Tina Fey as candidate Palin satirizes her interview with Katie Couric, played by Amy Poehler. Couric, through silent wide-eyed looks, can't quite believe Palin's answers, like this one about her visit to the United Nations: "I was disheartened by how many of them were foreigners. I promise when Sen. John McCain and I are elected, we are going to get those jobs back in American hands." Tina Fey/Sarah Palin, you are missed. — Claudine Zap (@zapkidd)
At this grill there is very little room for variety.
Hi boys and girls, it's Mr. Robinson!
Sean Connery, French Stewart, and Burt Reynolds square off.
Christopher Walken's "I gotta have more cowbell," is the line everyone remembers from this popular 2000 "SNL" spoof of Blue Oyster Cult on "VH1's Behind the Music." What's less well known is the wardrobe change that helped make it so memorable. Jimmy Fallon, who famously cracks up during the sketch, says the sketch wasn't as funny in dress rehearsal. Then Will Ferrell changed his shirt. Playing an overzealous cowbell player on "Don't Fear the Reaper," Ferrell's hard-working crop top brought hilarious ridiculousness. "When he lifted the cowbell, his gut came out of the bottom …. He's the funniest man on Earth," Fallon told Larry King. Enjoy the beloved moment and other classics from "Saturday Night Live."
Get something special for your girlfriend this Christmas.


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Pepsico Incorporated (PEP) maintained its quarterly dividend of 53.75 cents per share. The dividend is payable March 29, 2013, to shareholders of record on March 1, 2013.
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Saturday, October 22, 2005
© Copyright 2015 Clayton News Daily
Just accept criticismWho can forget the "How dare you question my patriotism?" claim that was routinely used leading up to last year's presidential election? Whenever a Bush supporter had the audacity to rebut a letter or column with a different opinion, that was the liberal's standard defense. This was often replaced with "You can't deprive me of my right to freedom of speech." Mike Bence, in his letter ("Swarm's nest quickly responds to attacks," To the Editor, Oct. 14), adds a third technique. Apparently he believes anti-Bush letters are so right-headed and reasonable that to express a different opinion is to somehow be unfair to those writers. Like it or not, when one puts their opinion in writing and submits it for printing, the result is often another writer with an opposing opinion will rebut it. Sometimes it can be brutal, and yes, sometimes it can be unfair. Liberals cannot claim to be exempt from critique because their opinions are so logical, pure and correct. Face it, when you put your opinions out there, the underlying purpose is to generate thought, influence opinion, add perspective or create discussion. - Johney R. Friar Auburn Bush not all-powerful There are many misguided people who have no concept of the office of the president, how our Congress works and how the business of the American government is done. These same people have repeatedly logged complaints and criticisms about President Bush and how he is running his administration. Recently there was talk as Bush is actively encouraging the building of new refineries. This has been long overdue, but unfortunately, the fault is not the president's. The reason no new refineries have been built in 30 years is due to the horrifically crushing regulations, specifically ridiculous and deliberately counter-productive "environmental" regulations that have been foisted on this country and its business climate by liberals who only seek to cripple our nation. You reap what you sow. Someone laughingly suggested that new oil refineries should be built on closed military bases, as if the president could snap his fingers and make this happen. The problem with this reasoning is manifold: It would put the government in the oil business as "competition" to existing companies; many military bases are not suited for the purpose, and most notably, some Americans are far too concerned with not-in-my-backyard issues and wouldn't actually want them there. Add this to the ludicrous number of specific gasoline formulations that must be made, again thanks to the enviro-whackos, and you have a perfect storm of reasons why the cost is up and why new refineries haven't been built. Much has been made recently of the enormous profits of the oil companies. Did anyone expect less? The petroleum business has more customers buying more oil than ever before. As most economists have already pointed out, the money amounts are due to more sales of more products, but the profit margin itself has not changed. It's amazing how little people know when the Internet is now available at our public libraries. Those who constantly carp about the state of our country should start doing something positive about it. President Bush was duly elected by a majority for his strong defense of our nation and the willingness to do what is right, even if it isn't popular. - Tony Rivera Suwanee
More like this story
- Letters to the Editor ( October 15, 2005 )
- Letters to the Editor ( September 23, 2005 )
- Less demand means fewer shortages, more gas ( October 3, 2008 )
- Why can't we get rid of the
oil vampires - Curt Yeomans ( September 12, 2008 )
- Letters to the Editor ( July 21, 2005 )


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Windsurfing News
- 04 August 2009 | Windsurfing
Cypriot star Michalis MALEKKIDES and Britain's Izzy HAMILTON were crowned the 2009 RS:X Youth World Champions after an action-packed week of racing in Turkey.
Scheduled to start just one week after the Volvo Youth Sailing ISAF World Championship finished in Buzios Brazil, the RS:X Youth World Championships - staged by the RS:X Class with the support of Palmalife, the Yalikavak local governement, the Turkish Sailing Federation and Turkish Airlines - was the biggest yet with 109 competitors taking part.
By the end of the competition, there was a perfect symmetry in the two RS:X Youth World Champions, Michalis MALEKKIDES (CYP) and Izzy HAMILTON (GBR) – both had won silver medals at the Volvo Youth Sailing ISAF World Championship just a couple of weeks earlier.
Seventy-eight youth men (including 27 under 17s) and 26 youth women (five under 17s) from 14 countries and three continents enjoyed champagne racing over six days. The TYF put in place a fantastic organization to manage the event with logistics being handled by Meet Travel.
Yalikavak built a mini-'Arc De Triomphe' in the centre of town in honour of the visiting youth athletes. Palmalife were a discreet but valued presence as the title sponsor without whom the major success of this championship would have foundered on the rocks of financial woes.
The event was covered by a four-man video production team and two stills photographers. Twenty-eight videos were loaded onto more than 10 video hosting sites. More than 1,000 images were loaded onto Flikr and received more than 15,000 views.
The Palmalife RS:X Youth Windsurfing Worlds website received more than 9,000 visits and 40,000 page views. Five races were run in under 10 knots of breeze, a further five in under 15 knots and the remaining three in 15 knots plus. These are dry statistics but they help to tell the story of the championships.
- 04 August 2009 | Windsurfing
British Airways will change their baggage policy from October 7th 2009.
When you fly with BA you can take certain types of sporting equipment in addition to your normal checked baggage allowance. If your equipment bags meet the dimensions and weight restrictions, they may be carried free of charge until 7 October 2009.
Baggage restrictions
In addition to your free checked baggage allowance, you are allowed to take one of the stated pieces of sporting equipment with you free of charge, provided it meets the following restrictions:
* Each bag must be no more than 240cm x 75cm x 65cm (94.5ins x 29.5ins x 25.5ins) including the handle, pockets and wheels. Sporting equipment listed below is exempt from the oversized bag charge. * Each bag must not exceed 23kg (51lbs). * Any bag that is more than 23kg (51lbs) and less than 32kg (70lbs) is subject to a charge at check-in. * If you have more bags to check in over your free allowance (including your one piece of equipment), these will be subject to an additional fee per bag.
- 03 August 2009 | Windsurfing
After six rounds of spectacular speed sailing Finian Maynard (BVI) is still leading the 2009 ISWC Speed Windsurfing World Championship. He is now three points ahead of Antoine Albeau (FRA) in second, with Anders Bringdal (SWE) in third, but already clear below. Bjorn Dunkerbeck (SUI, Starboard, Severne) and Hans Kreisel (NED) complete the Top 5.
Valerie Ghibaudo (FRA, Loft Sails) has won all heats of the womens competition and is ahead of Marion Raisi (FRA) and Marie-Paule Geldhof (BEL).
Tomorrows racing should allow for the second discard, which could mix up the rankings.
The actual results can be found here for download: Results after day 3
The day started with skippersmeeting at 10:00 and competition comenced at 11:00. Although the weather forecast was not promising, a complete round of silver and gold was completed before the lunch break. As the conditions became tricky at the end of the gold fleet heat, the AP was put up and the competitors put on a longer hold.
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Destiny's new live-action trailer is filled with space, futuristic weaponry, big bad monsters and snarky music choices. It's almost exactly like the actual game, except for that last bit – that's a little more Star-Lord, a little less Activision. It's definitely a lot more rock and roll, though.
Destiny is due out across PS3, PS4, Xbox 360 and Xbox One on September 9.
Buy from $298
- Key specs
- Prices
- Discussions
- Game format Optical disc, Downloadable
- Online features Multiplayer, Voice chat, Video chat, Store, Browser
- Drive capacity 250 GB
- Controller type Wired, Wireless
- Motion controls Accelerometer, Gyroscopic
- Video outputs HDMI (v1.3), RCA / composite
- Released 2012-09-25
Sony PlayStation 4
Microsoft Xbox One


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No Stranger To Group Sex, Kacey Jordan Sometimes Can't…
Views: 194 | Added: 3 years agoMoreWatch Video
If there's a group fucking, Kacey Jordan is there. What a slutty chick! She absolutely has a great time getting fucked in this video. Who wouldn't want to fuck that fuck hole? Kacey Jordan sure has a great time getting fucked. She also likes joining in on group sex.
Submitted by: mofos


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micoach is all you need to get the results you want.
(70 Products)
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Just bought this shoes this week and after 2 days it was delivered. Fast - super fast shipping. Been looking for this shoes and my size, luckily I was able to purchase it only. The quality is perfect and the style is vintage. With Adidas Stan Smith you won't go wrong baby! its off the hook shoes. I will pair this with my skinny and jogger pants. ... read more
Based from the reviews of many women about the size on this sneakers, I ordered size 6 kids and it worked for me. Was a little bit hesitant fighting between sizes 6-6.5 kids. I'm always 7.5 on sneakers and most of the regular shoes, women. I'm 5'5", 115 lbs and size 6 kids works great on me. I still have room on my toes and it is not tight at all. I'm so happy I bought this and I followed the reviews of the women here, now I'm thinking about getting a pink one with velco straps in 6 kids. ... read more
My partner swears by these trainers. Each year for Christmas and his birthday he gets a new pair and we have to rotate the colour. The trainer is excellently made and wears incredibly well. Even I brought myself a pair !! ... read more
These trainers look fab worn, they're a a modern look 70's style trainer. Very comfortable and light on your feet. Would like them to come in a few different widths. Would definitely recommend these trainers. Best buy I made! ... read more
I used to wear the all-white 'Originals' as a kid, so i had to get my hands on these! Love their simplicity and how they look casual but stylish. I originally bought the women's size in 6 1/2 (my original size) but they where way to big! I had to return them. So as recommended I went down a size and got a 4 1/2 in kids/youth. They fit perfectly. Def recommend going down a half or a full size! ... read more
I used to wear the all-white 'Originals' as a kid. I love their simplicity and the casual but stylish look of them. I originally bought a 6 1/2 women (my normal size) but they where way too big! Had to return. I went down a size like recommended and bought a 4 1/2 in Kids/Youth. I do recommend going down a size!! Fits perfectly!! Shipping took only 4 days for both pairs. ... read more
I'm normally a Woman size 7.0 and purchased these in size 5Y The top part of my pinky hurts a little when I have them on but I know these are meant for YOUTH which that might be the reason. Overall I love my superstars. Don't think about it twice before buying these. It took me over a week to find these online & In stores :) YOU WILL LOVE THEM! ... read more
Recently I went to Adidas and I was looking for Superstars *cough* of course. I stumbled across the Pharrell superstars and I fell in love with them. When I go to school or out everyone stares at them. I feel like most people do not like them. One of my close friends says they are trash and the others do not really have opinions for the shoes, but I absoloutly love them. When I walk down the hallways everyone seems to look at my shoes. I am now used to it for a second day. I must say that the shoes are worth the money because the are SOOO COMFY and COOl. I mean even if most people do not like them, I LOVE THEM! ... read more
I can get cheap clothes but I never get cheap shoes because foot comfort is essential to a runner who has back pain like me. I love the look of these shoes, they are classic. But they also feel good on my feet after standing four hours in front of a classroom. So good all the way. Good surprise. ... read more
Always stuck with what I like but decided for a change this time and the superstars stood out straight away. 10/10 for looks 9/10 for comfort & this is only because for the first few days there's an uncomfortable lump on the inside centre of the trainer but once I got use to the feel of a different trainer I don't even know I've got them on ... read more
This is my first stan smith shoe and also my first white sneaker. I used to wear shoes with dark colors and my favorite color is always black. But this one totally change my mind. It looks so stunning and matches with most of my clothes! I love it. ... read more
too big for my usual size. but it's ok since it's good to have an allowance on my big toe. looks good. had a problem with shipping (charged with shipping fee) but customer service refunded it to me. so all is well. ... read more
I'm a size 6.5 in women, I first tried a size 5 and they were too large so I went for a 4.5 and they are perfect. So, I recommend 2 sizes down for women. I'm normally a 37 euro and the tricky thing is that size 5 is 37+1/3 and size 4.5 is 36+2/3 so you could be 1.5 sizes down or 2... depends on how fluffy your socks are too. ... read more
I've had countless pairs of Superstars over the years, but these are particularly comfy. I wouldn't normally go for something as stylish as these (I usually prefer more plain styles) but I fancied a change and got them personalised with my kids names on them. Delivery was pretty speedy for being customised too. ... read more
I've been looking for these shoes everywhere and now that I've got them I'm so happy!!! At first I bought a 7 for men as I am normally an 7.5-8 in women, but these were EXTREMELY big. I returned them and started looking at reviews and different size charts. I ended up ordering them in 5.5 men, which is under the category youth/kids. They fit PERFECTLY!!! ... read more
I am size 7 in woman and i choose 4.5 in kids, it's fit with me, a little bit big, so i have to tight lace tightly, wear socks ( not soft socks), that's good. maybe you should buy size 4 , if not fit, u can exchange size 4.5 ... read more
I always go for classic shoes and Stan Smith is just one of those that will never get out of style. My size US W-8.. I got big kids 6.5 this is comfortable with a little allowance.. I can also fit in size 6... that's how big are the kids..also saved me a little money compared to adult shoes ... read more
I usually do not write reviews unless I feel compelled to praise something and honestly these shoes are incredible. I am thirty year old and been wearing adidas since I was a little kid and have always been a huge shoe buff. I have never owned the superstar style and definitely not the skate version so wasn't really sure what to expect. I was blown away with how nice they are. They are super well made, incredibly comfortable, and look killer. A lot of shoe companies will say they have this and that added for comfort but wasn't really expecting anything extraordinary. But these really are extraordinarily comfortable, the insole is so plush and soft. TL;DR These shoes are awesome. ... read more
REAL LEATHER!...silver! classic Stan Smith style with velcro bands. These were SO popular, they became an icon for active seniors! Awesome as a dress shoe.All over Paris , France. oooops! have a men's 10.5>need 11.5....anyone? love them 5***** ... read more
Bought it online, delivered on time. I cannot find blue stripe colour in high street. Been waiting for it for a long time . Bought it as present for my husband who refused to wear shoes other than superstar adidas . He is very delighted with the colour and so comfy and he said he can walk thousand steps without pain on his feet and calves....plus I have a 15% off. Thanks Adidas. Wish you will produce green stripe next time. ... read more
love these shoes - bought for friend as a birthday present and she really loves them. only slight thing is they took a day longer to arrive than it said but they came in perfect condition so can't complain ... read more
First of all, I have to say they are perfect to build up different styles, vintage, sports, casual, semi-causl. it most could work for most of our daily occations. Secondly, i want to recommend that maybe it should have 1/2 size, because it is a unisex product following us men's size, it is awkward for some girls to select their suitable size ... read more
Great value for money. Shoes fit perfectly. Highly recommend these shoes for the following reasons: Durability - 10/10 Comfort - 10/10 Style - 10/10 Don't need to write anymore as these bad boys speak for themselves. Best shoes ever!!~ ... read more
Hopefully I can help with the people out there who are wondering what size to get in this shoe with the child size. I was in the store today and tried on multiple pairs of the Stan Smith (LOVE these. Classic. Timeless. Comfortable. Amazing.) I wore these in my younger years and they are so durable, comfortable, stylish, etc. etc. I normally wear a 7.5 womens shoe. I bought the 5.5 and they are absolutely PERFECT. Yes, the women's sizes do run big. I also purchased another pair of Stan's in a women's size 6. Perfect fit...... I hope this helps. I love you ADIDAS!! ... read more
These shows are not only really good looking, but they fit perfectly! They're super comfortable and give a lot of arch support and I honestly just feel good when I wear them. I highly recommend them, these are my favorite shoes ... read more


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Respond to Review of Garden Cafe of Woodstock
by JennyB
Review: As ethical & health-conscious vegans living in Woodstock - life is complete for us now! We love Garden Cafe--from it's creative and filling meals to it's welcoming and tastefully designed decor (now complete with a beautiful outdoor dining area!) The menu is varied, inventive and not overpriced and the owner, Pam Brown, puts a tremendous amount of care and energy into every detail. My husband and I are steady regulars and it's a favorite for many friends in town - veg and non-veg. We would go every night if we could! For a restaurant just closing in on it's first full year of operation, we're extremely impressed by the ongoing improvements we've seen in every aspect of the operation. Things just keep getting better & better. Too many favorites to list here but make sure to try the Strawberry Shortcake! THUMBS WAY UP!
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Grove City oh heating & cooling repair & installation
Over 12 years of experience & technical expertise, customer satisfaction & service that is second to none. We'll make sure you're as comfortable as possible with your home's heating & cooling system.
In a large sea of "best value" and "lowest price", it may be confusing to choose a service company that is sincere and offeres quality work. We are not going to talk about limited time offers and restrictions that may apply. We will just give you the facts and let you decide if we are the company for you.
As a company, we think of ourselves as the good guys. We can always be super heros, but we can do our best everyday to improve the comfort level of our clients in Grove City OH and surrounding areas.
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I love my ssk8. Try xtol straight or 1:1 it works so much better than anything I get back from the pro labs. Also I find xtol not to fiddly for timing when throwing different films at it.
turn down rotational speed to minimum. Have yet to lose a 120 roll so far
4 to 4:30 works great for most bw films in xtol 1:1


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Readingtime: 4 minutes
Global toy sales rise 7%; DIY SOS announced for toys and games; ... Read the most important news and announcements of the toy industry from 16 Nov - ...
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The expansion and ubiquity of smartphones are shaping not only the way we communicate but also the way we play. The cultural aspects of it are...
Star Wars is more than a movie, it is the name of some of the most valuable collectible toys on the planet, an international phenomenon unlike any...
These dates in the toy industry you should remember in November 2015!
Star Wars & other movies are driving the toy industry up
These dates in the toy industry you should remember in October 2015!
25. November 2015 | 16:21
U.S.: NRF predicts Top Toys for holiday 2015
25. November 2015 | 14:13
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WAF 210: Welding Metallurgy
In this course, students will study the fundamental behaviors and properties of ferrous and nonferrous metals. Students will prepare samples for macro and micro inspection to identify different grain structures and properties. Following the American Welding Society codes and specifications, students will be able to recognize, repair, troubleshoot, and determine various weld procedures.
Level I Prereq: Academic Reading and Writing Levels of 6; Academic Math Level 1; WAF 112 and WAF 215, minimum grade "C"; both courses may enroll concurrently
- Credits
- 3
- Lecture Hours
- 45
- Clinical Hours
- 0
- Lab Hours
- 15
- Other Hours
- 0
- Total Hours
- 60
Some classes are not offered every term. To see if this class is currently available, check the schedule.
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Save a trip to campus. Just fire up your computer webcam and microphone to talk to an advisor online.more »
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What Students Say About Online Classes
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CHELMSFORD, Mass.--(BUSINESS WIRE)--Axis Communications, the world leader in network video surveillance, today unveils AXIS P5414-E PTZ Dome Network Camera, the first in a new line of uniquely designed outdoor-ready pan/tilt/zoom domes with "direct drive" technology meant for wall-mounting. The new HDTV 720p-quality PTZ dome with 18x optical zoom is ideal for use in city and perimeter surveillance applications, parking garages, campuses and schoolyards, and outside of retail and medical buildings.
The intelligent direct drive pan/tilt system found in AXIS P5414-E facilitates easy wall-mount installation and supports mechanical PTZ endurance by limiting the amount of moving parts. The camera is directly attached to the drive shaft of two motors – one for panning and another for tilting the camera. With no mechanical parts such as belts or gears, AXIS P5414-E creates a durable and highly efficient pan/tilt system to provide customers with a reliable and robust PTZ camera. Other innovations include a built-in wall bracket that eliminates the need to purchase a separate mounting accessory, which provides additional cost-savings and makes installation quicker and easier. See demo movie.
With the ability to pan 270°, AXIS P5414-E can also be mounted on the corner of a building using a corner mount bracket to enable wide area coverage and high optical zoom for detailed surveillance in HDTV resolution.
"About half of all PTZ cameras [installed today] are mounted on walls. AXIS P5414-E addresses this market with a unique design," says Erik Frännlid, director of product management, Axis Communications. "The result is an attractively priced, high-performance HDTV PTZ camera that's easy to install."
AXIS P5414-E features an integrated sunshield designed for easy removal, allowing customers to paint it to match the installation environment. This sunshield's sloping form helps the camera resist attempts by vandals to pull it down. The camera has a hidden junction box that supports two-way audio, a memory card slot, four configurable inputs/outputs to external devices, and the option to use either 24 V DC or Power over Ethernet Plus (IEEE 802.3at). AXIS P5414-E also supports Advanced Gatekeeper, which enables the camera to automatically pan, tilt and zoom to a preset position when motion is detected in a predefined area.
AXIS P5414-E is expected to be available in July 2013 through Axis distribution channels at the suggested retail price of $1599.
Features of AXIS P5414-E include:
- HDTV 720p performance in compliance with the SMPTE 296M standard for resolution, full frame rate, HDTV color fidelity and 16:9 format.
- 18x zoom with autofocus in combination with HDTV resolution delivers the advantage of a wider (16:9) field of view while providing zoomed-in views with the same level of detail as a standard resolution, 36x-zoom camera.
- Multiple, individually configurable streams in H.264 and Motion JPEG video formats.
- True day/night functionality with an automatically removable infrared(IR)-cut filter for color video during day time, and high-quality black and white video in low-light.
- Intelligent video functionalities such as video motion detection, Advanced Gatekeeper and audio detection, as well as support for additional applications through AXIS Camera Application Platform.
- Outdoor-ready with IP66 and NEMA 4X ratings that ensure protection against dust, rain, snow and corrosion.
AXIS P5414-E will be supported by the industry's largest base of video management software through the Axis Application Development Partner Program, AXIS Camera Station and the complimentary AXIS Camera Companion. The camera also includes support for AXIS Video Hosting System and ONVIF for easy camera system integration.
For photos and other resources, please visit:
About Axis Communications
As the market leader in network video, Axis is leading the way to a smarter, safer, more secure world – driving the shift from analog to digital video surveillance. Offering network video solutions for professional installations, Axis' products and solutions are based on an innovative, open technology platform.
Axis has more than 1,400 dedicated employees in 41 locations around the world and cooperates with partners covering 179 countries. Founded in 1984, Axis is a Sweden-based IT company listed on NASDAQ OMX Stockholm under the ticker AXIS. For more information about Axis, please visit our website www.axis.com.


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Welcome to Ainsley's Angels of America
Keep the Race going!
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Working together for a common goal.
Upcoming race information.
The precious girl that inspired a nation.
Read more...
"Our daily positive themes - Motivation Monday - Teamwork Tuesday - Writing Wednesday Thoughtful Thursday - Fun Filled Friday - Story's Behind The Bib Saturday - Spirit Sunday "
Together, we shall accomplish what we might have once thought unachievable.
... Kim Rossiter
There Is power and inspiration In wanting to be the legs for someone who can't fully use their own.
You have never really lived until you have done something for someone who can never repay you.
The real purpose of running isn't to win a race, it's to test the limits of the human heart
- Bill Bowerman


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(based on 7 reviews)
Ratings Distribution
5 Stars
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of respondents would recommend this to a friend.
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Reviewed by 7 customers
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By shadowcatAZ
from AZ
About Me Bargain Hunter
Comments about Cobalt Blue Depression Style Glass Cat Candy Dish:
It's very pretty. I like the simple design, and it looks nice on my bookcase. The lid doesn't fit the base as well as it should so it's a little unstable. The "cat" part is wobbly, so it's on a shelf in a place where it's unlikely to be bumped. I love the overall design, and I wish it came in some other colors.
Bottom LineYes, I would recommend this to a friend
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So cute!
By BD
from FL
About Me Practical
I collect all kind of unique cat gadgets... I already have a pink cat candy dish, identical in shape to this blue one, so this was simply an expansion of my candy cat dish collection. :) Glass is heavy and sturdy, and looks beautiful whether in my curio or on my countertop, holding candy. Would like this item being available in more colors.
(1 of 1 customers found this review helpful)
It was in pieces when I got it all broke
By LOLO
from Vancouver WA
Would not know it was all broken when I got it and would have cost me $12.00 to sent it back almost as much as the dish itself
Bottom LineNo, I would not recommend this to a friend
Beautiful addition to my collection
By Katbalu
from New Mexico
About Me Stylish
Since I love cobalt blue glass and cats, this was a natural for my collection and I love it. The only problem was that it has a small chip on the rim of the dish. I'm afraid to use it for candy for fear someone may cut themselves. So, I will just look at it and enjoy it.
Very Sweet
By Ladybug2535
from Portland, OR
I'm using this adorable container to store my artificial sweetener packs. It works perfectly and looks decorative.
I love it very nice
By Snooks
from Colorado springs
Very nice
(0 of 1 customers found this review helpful)
Sleeping Kitty
By physprof
from Akron, OH
This fits in well with my cobalt blue glass collection.


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Sol a Gogo Campsite
St Hilaire-de-Riez, VendeeSite code: VN016
Parc Summary
- Close to lively resorts including Saint-Hilaire-de-Riez and Saint-Jean-de-Monts
- Take the parc's private path to the beach next door
- Hop on a bike and explore a number of nearby cycle routes
Roman themed, and great for roaming by bike
Where else on the Vendée can you get a thoroughly modern parc with direct beach access and a pool with a quirky, Roman ruins-theme?
Stay at Sol a Gogo and it's not only a superb pool complex with water slides, covered pool and Jacuzzi that will keep everyone occupied, there's direct access to a wonderful, sandy beach, as well as a host of kids' activities that are organised on parc. Because of its beach-side location, pitches on this parc are sandy and on the smaller side. In high season the 'animateur' organises a range of activities including regular discos in the bar. Stay at Sol a Gogo and you'll be treated to fun, laughter and a little ancient history thrown in for good measure!
Extras with Eurocamp
- Linen Hire
- Eurocamp Couriers on site
- Gas BBQ provided free of charge
- Welcome pack
- Games
General Information
Parc size
Small - 197 Pitches:
Opening Dates:
20/05/2016 - 03/09/2016
Nearest Airport:
Nantes (NTE) - 86km
Nearest Port:
St Malo (STM) - 265km
Nearest Beach:
Direct Access
Nearest Town:
Saint-Hilaire-de-Riez - 6km


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The Juvenile Refraction
A compilation of photography by Chantel from the age of 15-17
by Chantel Beam
Chantel's first book. The photos range from portraits to still life and all in between. They were taken beggining at the age of 15 to 17.
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An enegmatic blend of creative angst and passion, Leoh's music is her way of being the change she'd like to see in the world. Though a talented musician with her own sound, collaboration with fellow musicians has allowed her to explore her creativity to give a selection of songs with differing sounds and context. Singing in the new energy, Leoh has honest vocals, lyrics that confront, challenge and desire to inspire.


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RAPID Projects on Hurricane Sandy
Mon. 11:30 a.m. to 12:30 p.m., Interlocken B
Hurricane Sandy might be the second most expensive weather-related hazard in the United States, following Hurricane Katrina in 2005. This session will discuss three National Science Foundation Rapid Response Research (RAPID) grant projects that explore varying aspects of Hurricane Sandy. Topics include communication and comprehension of surge forecasts and warnings, postdisaster risk during short-term recovery, and short-term emergent groups and aid mobilization.
Rob Schwartz, Presenter
University of Akron
Betty Morrow, Presenter
SocResearch Miami
Ken Mitchell, Presenter
Rutgers University
Organizer: Rob Schwartz, University of Akron


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Chefs Veg Out: Chadae Rickett of Kushi
Short ribs and baby octopus may dominate many menus in the city, but that doesn't mean local chefs can't find love in an acorn squash. In our ongoing series, Chefs Veg Out, we'll prove D.C.'s chefs can play with more than just meat.
Name: Chadae Rickett Title: Sous chef Restaurant: Kushi Izakaya & Sushi Twitter: @eatkushi Cooking Since: Eight years ago. "I went to Johnson and Wales [University] in Charlotte [N.C.] and cooked in Alexandria and Fairfax. I'm a local." Random Fact: "I'm a really picky eater: no mushrooms, no shellfish, not big on pork or steak. The list goes on. And nothing really raw." (How do you work at Kushi? "We have good grilled food too.") Favorite Vegetable: Potatoes. "I loved mashed potatoes. Everything except for hash browns or tater tots. It's the texture. Something about minced potatoes formed feels weird."
Least Favorite Vegetable: Mushrooms. "The flavor, smell, taste, everything. They're kinda weird 'cause you're eating dirt." Memorable Meatless Dish: Green bean casserole. "My dad made it for me every year for my birthday since I was probably 7. It's so easy and so cheap: canned green beans, fried onions, mushroom soup. It's the only time I'll eat mushrooms. And I still get it every year for my birthday and Thanksgiving." Best Vegetable Dish at Kushi: "I really like the kinpira burdock root. It's a root that comes from Japan that we julienne and slow cook in sake, mirin, and sugar. It's surprisingly sweet and really good. We serve it chilled and top it with white and black sesame seeds." Quick and Dirty Meatless Idea: Grilling. "Asparagus is best when it's grilled with just olive oil, salt and pepper. Throw it on a hot grill for a few minutes and it's tasty."
Photo courtesy ThreeLockharts Communications


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RegionalCare's Rash giving up CEO role
RegionalCare Hospital Partners co-founder Marty Rash will at the end of the month relinquish his CEO title to Kent Wallace, who is leaving his president and COO job at fellow local hospital chain Vanguard Health Systems. Wallace also will fill the COO role at RegionalCare now held by John Rutledge, who is leaving the company he helped launch in mid-2009 for another, unnamed venture.
For 57-year-old Wallace, the move to RegionalCare means a reunion with Rash, with whom he worked at the former Province Healthcare as a regional vice president from July 2001 to December 2002. He has been with Vanguard since September of 2005 and was its Texas market president for three years before being promoted to his current posts.
"Kent is one of the leading healthcare service executives in the country and brings a tremendous depth of experience and proven leadership," Rash said in a statement. "I look forward to working closely with Kent to accelerate the growth of RegionalCare's seven-hospital system and to expand its geographic presence across the United States."
Starting next month, Rash's title will be executive chairman and he will continue to be involved in RegionalCare's strategy and business development efforts. RegionalCare last month announced it had signed a letter of intent with executives at Sierra Vista Regional Health Center in southeast Arizona for a long-term lease and the construction of a new 100-bed hospital.
At Vanguard, Chairman and CEO Charlie Martin also will take on Wallace's president role come March 1. In a filing with the Securities and Exchange Commission, Vanguard said it has waived the non-compete clause in Wallace's contract but that the executive will have to abide by non-solicitation and confidentiality clauses.


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The Signs and Symptoms of Teenage Stress
In today's competitive society, stress is something commonly found in a teenagers life. Along with the competitive society, teens are also going through some dramatic changes in their life that make them feel odd and, as a result, add even more stress. Each day they are trying to adapt to changes in their life, school, school activities, social life, and, much more. However, for some teens this stress can become so overwhelming that it disrupts their everyday life. To better understand teen stress and how to deal with it, both teens and parents need to become aware of the signs any symptoms.
Physical Symptoms
A lot of times stress in a teen's life can lead to a variety of different physical symptoms. Although occasional stress in a teen's life only causes temporary symptoms that quickly go away once the stress disappears, there is a dangerous level that teens and parents need to be aware of. This dangerous level can lead to a wide variety of different physical symptoms which occur quite frequently. If you find that your teen is experiencing these physical more often than normal, than they may have a dangerously high level of stress in their life and should have some kind of counseling.
Mental Symptoms
A lot of times in a teens life stress can cause a variety of different mental symptoms. However, prolonged stresses in a teen's life can lead to such things as forgetfulness, low concentration, and others. Also lower grades than average can also be a sign of these symptoms and, can suggest that the teen has a high amount of stress in their life. Although it is common for some teens to have this type of stress in their life, if it is prolonged it can cause big problems.
Emotional Symptoms
What a lot of parents may not realize is that stress can have a major effect on their teens emotional health. Stress can lead to a variety of different symptoms including; sudden bursts of anger, crying fits, and easily irritated among others. However, if your teen seems to be having problems controlling these symptoms they may have severe stress in their life. If your child has frequent screaming fits, or shows signs of violence their stress may be too high.
Disorder Symptoms
When your teen suffers from a high level of stress it can quickly lead to a variety of different disorder symptoms. Things such as insomnia are often signs that your child is suffering from too much stress. Frequent nightmares is also a big trigger caused by too much stress. If your teen shows any of these symptoms you should consult your physician for a referral to a counselor.


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Clinical Focus
- Allergy and Immunology
- Aspirin Desensitization
- Asthma
- DiGeorge Syndrome
- Food Allergies
- Primary Immunodeficiency Diseases
- 22q11 Deletion Syndrome
Academic Appointments
Clinical Associate Professor, Pediatrics - Immunology and Allergy
Administrative Appointments
Clinical Assistant Professor, Stanford Hospital and Clinics (2011 - Present)
Clinical Assistant Professor, Lucile Packard Children's Hospital (2011 - Present)
Medical Director, Allergy & Immunology Clinics (2011 - Present)
Program Director, Allergy & Immunology Fellowship Program (2011 - Present)
Honors & Awards
Cecil H. Short Prize, Pomona College (1992)
Vaile Prize, Pomona College (1992)
Alice Littman Moss Award, UCLA Pediatric Residency Program (2001)
Centocor Immunology/Rheumatology Scholar Award, Western Society for Pediatric Research (2004)
Professional Education
Fellowship:David Geffen School of Medicine at UCLA (2005) CA
Residency:David Geffen School of Medicine at UCLA (2002) CA
Internship:David Geffen School of Medicine at UCLA (1999) CA
Medical Education:Stanford university School of Medicine (1998) CA
Board Certification: Pediatrics, American Board of Pediatrics (2001)
Board Certification: Allergy and Immunology, American Board of Allergy and Immunology (2005)
Community and International Work
Painted Turtle Summer Camps
Summer camps for children with chronic disease
Ongoing Project
Opportunities for Student Involvement
Madisons Foundation
Pediatric rare diseases
Populations Served
Patients, families and loved ones with rare pediatric diseases
Ongoing Project
Opportunities for Student Involvement
Current Research and Scholarly Interests
2015-16 Courses
Independent Studies (5)
- Directed Reading in Pediatrics
PEDS 299 (Win, Spr)
- Early Clinical Experience
PEDS 280 (Win, Spr)
- Graduate Research
PEDS 399 (Win, Spr)
- Medical Scholars Research
PEDS 370 (Win, Spr)
- Undergraduate Directed Reading/Research
PEDS 199 (Win, Spr)
- Directed Reading in Pediatrics
Stanford Advisees
Postdoctoral Faculty Sponsor
Cathleen Collins
Graduate and Fellowship Programs
All Publications
Large deletions and point mutations involving the dedicator of cytokinesis 8 (DOCK8) in the autosomal-recessive form of hyper-IgE syndrome
2009; 124 (6): 1289-1302
The genetic etiologies of the hyper-IgE syndromes are diverse. Approximately 60% to 70% of patients with hyper-IgE syndrome have dominant mutations in STAT3, and a single patient was reported to have a homozygous TYK2 mutation. In the remaining patients with hyper-IgE syndrome, the genetic etiology has not yet been identified.We aimed to identify a gene that is mutated or deleted in autosomal recessive hyper-IgE syndrome.We performed genome-wide single nucleotide polymorphism analysis for 9 patients with autosomal-recessive hyper-IgE syndrome to locate copy number variations and homozygous haplotypes. Homozygosity mapping was performed with 12 patients from 7 additional families. The candidate gene was analyzed by genomic and cDNA sequencing to identify causative alleles in a total of 27 patients with autosomal-recessive hyper-IgE syndrome.Subtelomeric biallelic microdeletions were identified in 5 patients at the terminus of chromosome 9p. In all 5 patients, the deleted interval involved dedicator of cytokinesis 8 (DOCK8), encoding a protein implicated in the regulation of the actin cytoskeleton. Sequencing of patients without large deletions revealed 16 patients from 9 unrelated families with distinct homozygous mutations in DOCK8 causing premature termination, frameshift, splice site disruption, and single exon deletions and microdeletions. DOCK8 deficiency was associated with impaired activation of CD4+ and CD8+T cells.Autosomal-recessive mutations in DOCK8 are responsible for many, although not all, cases of autosomal-recessive hyper-IgE syndrome. DOCK8 disruption is associated with a phenotype of severe cellular immunodeficiency characterized by susceptibility to viral infections, atopic eczema, defective T-cell activation and T(h)17 cell differentiation, and impaired eosinophil homeostasis and dysregulation of IgE.
View details for DOI 10.1016/j.jaci.2009.10.038
View details for Web of Science ID 000273071500022
View details for PubMedID 20004785
Newborn screening for severe combined immunodeficiency and T-cell lymphopenia in California: Results of the first 2 years
Assay of T-cell receptor excision circles (TRECs) in dried blood spots obtained at birth permits population-based newborn screening (NBS) for severe combined immunodeficiency (SCID).We sought to report the first 2 years of TREC NBS in California.Since August 2010, California has conducted SCID NBS. A high-throughput TREC quantitative PCR assay with DNA isolated from routine dried blood spots was developed. Samples with initial low TREC numbers had repeat DNA isolation with quantitative PCR for TRECs and a genomic control, and immunophenotyping was performed within the screening program for infants with incomplete or abnormal results. Outcomes were tracked.Of 993,724 infants screened, 50 (1/19,900 [0.005%]) had significant T-cell lymphopenia. Fifteen (1/66,250) required hematopoietic cell or thymus transplantation or gene therapy; these infants had typical SCID (n = 11), leaky SCID or Omenn syndrome (n = 3), or complete DiGeorge syndrome (n = 1). Survival to date in this group is 93%. Other T-cell lymphopenic infants had variant SCID or combined immunodeficiency (n = 6), genetic syndromes associated with T-cell impairment (n = 12), secondary T-cell lymphopenia (n = 9), or preterm birth (n = 8). All T-cell lymphopenic infants avoided live vaccines and received appropriate interventions to prevent infections. TREC test specificity was excellent: only 0.08% of infants required a second test, and 0.016% required lymphocyte phenotyping by using flow cytometry.TREC NBS in California has achieved early diagnosis of SCID and other conditions with T-cell lymphopenia, facilitating management and optimizing outcomes. Furthermore, NBS has revealed the incidence, causes, and follow-up of T-cell lymphopenia in a large diverse population.
View details for DOI 10.1016/j.jaci.2013.04.024
View details for Web of Science ID 000321052300019
View details for PubMedID 23810098
Use and interpretation of diagnostic vaccination in primary immunodeficiency: A working group report of the Basic and Clinical Immunology Interest Section of the American Academy of Allergy, Asthma & Immunology
A major diagnostic intervention in the consideration of many patients suspected to have primary immunodeficiency diseases (PIDDs) is the application and interpretation of vaccination. Specifically, the antibody response to antigenic challenge with vaccines can provide substantive insight into the status of human immune function. There are numerous vaccines that are commonly used in healthy individuals, as well as others that are available for specialized applications. Both can potentially be used to facilitate consideration of PIDD. However, the application of vaccines and interpretation of antibody responses in this context are complex. These rely on consideration of numerous existing specific studies, interpolation of data from healthy populations, current diagnostic guidelines, and expert subspecialist practice. This document represents an attempt of a working group of the American Academy of Allergy, Asthma & Immunology to provide further guidance and synthesis in this use of vaccination for diagnostic purposes in consideration of PIDD, as well as to identify key areas for further research.
View details for DOI 10.1016/j.jaci.2012.07.002
View details for Web of Science ID 000308464300001
View details for PubMedID 22935624
Public health comes to immune deficiency
2012; 119 (11): 2433-2435
View details for DOI 10.1182/blood-2011-12-397836
View details for Web of Science ID 000301941700005
View details for PubMedID 22422811
How the practice of allergy shows the promise and challenge of personalized medicine
2011; 104 (1-2): 3-6
Personalized medicine seeks to stratify therapies according to individual characteristics, and by so doing improve effectiveness and reduce complications. However, there are not many models of care that is highly stratified within a single diagnosis in this manner. One potential model is the practice of allergy, in which care is tailored to specific allergens for individual patients within the broader context of care for rhinitis or asthma. Allergists have already confronted many of the same regulatory issues anticipated for personalized medicine. The history of allergy practice also anticipates some of the patient safety concerns that may arise from tracking and using highly personalized medical information. Finally, the therapy of allergy and asthma has been at the forefront of attempts to incorporate pharmacogenomics information into patient care. Individualized therapy has always been central to the practice of allergy, and so provides a useful proving ground for personalized medicine as a concept of care.
View details for DOI 10.1016/j.ymgme.2011.07.017
View details for Web of Science ID 000295151300002
View details for PubMedID 21810545
DOCK8 immune deficiency as a model for primary cytoskeletal dysfunction
2010; 29 (3-4): 151-156
DOCK8 deficiency is a newly described primary immune deficiency resulting in profound susceptibility to cutaneous viral infections, elevated IgE levels, and eosinophilia, but lacking in the skeletal manifestations commonly seen in hyper IgE syndrome, which it otherwise resembles. Although little is known about the DOCK8 protein, it resembles other atypical guanine exchange factors in the DOCK family, and is known to bind to CDC42. This suggests that a likely role for DOCK8 is in modulating signals that trigger cytoskeletal reorganization. As a result, DOCK8 may also be related to other immune deficiencies that involve the cytoskeleton and Rho GTPase signaling pathways, such as Wiskott-Aldrich syndrome and Rac2 deficiency.
View details for DOI 10.3233/DMA-2010-0740
View details for Web of Science ID 000285458400005
View details for PubMedID 21178274
Immunologic reconstitution in 22q deletion (DiGeorge) syndrome
2009; 45 (1): 37-45
Adoptive transfer of mature T cells (ATMTC) through bone marrow (BM) transplantation, first attempted over 20 years ago, has recently emerged as a successful therapy for complete 22q deletion syndrome (22qDS). This provides a potential option to thymic transplantation (TT) for immune reconstitution in 22qDS. Compared to thymic transplant, ATMTC is an easier procedure to accomplish and is available at more centers. However, there are differences in the nature of the T-cell reconstitution that results. Predictably, more naïve T cells and recent thymic emigrants are present in patients treated with thymus transplant. There are no significant differences in mortality between the two procedures, but the number of patients is too limited to conclude that the procedures are equally effective. Adoptive transfer should be pursued as a reasonable treatment for 22qDS patients requiring immune reconstitution when thymus transplant is not available.
View details for DOI 10.1007/s12026-009-8108-7
View details for Web of Science ID 000269904000004
View details for PubMedID 19238335
Defects along the T(H)17 differentiation pathway underlie genetically distinct forms of the hyper IgE syndrome
2009; 124 (2): 342-348
The hyper IgE syndrome (HIES) is characterized by abscesses, eczema, recurrent infections, skeletal and connective tissue abnormalities, elevated serum IgE, and diminished inflammatory responses. It exists as autosomal-dominant and autosomal-recessive forms that manifest common and distinguishing clinical features. A majority of those with autosomal-dominant HIES have heterozygous mutations in signal transducer and activator of transcription (STAT)-3 and impaired T(H)17 differentiation.To elucidate mechanisms underlying different forms of HIES.A cohort of 25 Turkish children diagnosed with HIES were examined for STAT3 mutations by DNA sequencing. Activation of STAT3 by IL-6 and IL-21 and STAT1 by IFN-alpha was assessed by intracellular staining with anti-phospho (p)STAT3 and -pSTAT1 antibodies. T(H)17 and T(H)1 cell differentiation was assessed by measuring the production of IL-17 and IFN-gamma, respectively.Six subjects had STAT3 mutations affecting the DNA binding, Src homology 2, and transactivation domains, including 3 novel ones. Mutation-positive but not mutation-negative subjects with HIES exhibited reduced phosphorylation of STAT3 in response to cytokine stimulation, whereas pSTAT1 activation was unaffected. Both patient groups exhibited impaired T(H)17 responses, but whereas STAT3 mutations abrogated early steps in T(H)17 differentiation, the defects in patients with HIES with normal STAT3 affected more distal steps.In this cohort of Turkish children with HIES, a majority had normal STAT3, implicating other targets in disease pathogenesis. Impaired T(H)17 responses were evident irrespective of the STAT3 mutation status, indicating that different genetic forms of HIES share a common functional outcome.
View details for DOI 10.1016/j.jaci.2009.05.004
View details for Web of Science ID 000268860400023
View details for PubMedID 19577286
Immunoglobulin Replacement Therapy in Children
2008; 28 (4): 833-849
The benefit of immunoglobulin (IG) replacement in primary antibody deficiencies is unquestionable. Many of these congenital disorders present early in life and this therapy is often first implemented in the young. This article focuses on the indications of IG replacement in children, with an emphasis on the specific diagnostic problems encountered in this population. Also presented is an overview of the practical aspects of IG administration in the pediatric setting, including the recognition and management of adverse reactions. Finally, the advent of subcutaneous IG, a therapeutic IG modality with the potential to have a great impact on the quality of life of children with antibody deficiencies and their families, is discussed.
View details for DOI 10.1016/j.iac.2008.07.001
View details for Web of Science ID 000261161800010
View details for PubMedID 18940577
Representational oligonucleotide microarray analysis (ROMA) and comparison of binning and change-point methods of analysis: Application to detection of de122q11.2 (DiGeorge) syndrome
2008; 29 (1): 176-181
DiGeorge (del22q11.2) syndrome is estimated to occur in 1:4,000 births, is the most common contiguous-gene deletion syndrome in humans, and is caused by autosomal dominant deletions in the 22q11.2 DiGeorge syndrome critical region (DGCR). Multiple microarray methods have been developed recently for analyzing such copy number changes, but data analysis and accurate deletion detection remains challenging. Clinical use of these microarray methods would have many advantages, particularly when the possibility of a chromosomal disorder cannot be determined simply on the basis of history and physical examination data alone. We investigated the use of the microarray technique, representational oligonucleotide microarray analysis (ROMA), in the detection of del22q11.2 syndrome. Genomic DNA was isolated from three well-characterized cell lines with 22q11.2 DGCR deletions and from the blood of a patient suspected of having del22q11.2 syndrome, and analyzed using both the binning and change-point model algorithms. Though the 22q11.2 deletion was easily identified with either method, change-point models provide clearer identification of deleted regions, with the potential for fewer false-positive results. For circumstances in which a clear, a priori, copy-number change hypothesis is not present, such as in many clinical samples, change-point methods of analysis may be easier to interpret.
View details for DOI 10.1002/humu.20593
View details for Web of Science ID 000252143300024
View details for PubMedID 17694540
Long-term results of bone marrow transplantation in complete DiGeorge syndrome
2007; 120 (4): 908-915
Therapeutic options for DiGeorge syndrome (DGS) with profound T-cell deficiency are very limited. Thymic transplantation has shown promising results but is not easily available. Hematopoietic cell transplantation (HCT) has been successful in restoring immune competence in the short term.Present the long-term follow-up of 2 patients with complete DGS who received bone marrow transplants in the neonatal period from HLA-matched siblings, and perform a multicenter survey to document the status of other patients with DGS who have undergone HCT.Immune function assessment by immunophenotyping, lymphocyte proliferation, T-cell receptor excision circles, single nucleotide polymorphism mapping arrays, spectratyping, cytogenetics, and fluorescence in situ hybridization were used.Among reported patients with DGS receiving HCT, survival is greater than 75%. Our patients are in their 20s and in good health. Their hematopoietic compartment shows continuous engraftment with mixed chimerism, normal T-cell function, and humoral immunity. Circulating T cells exhibit a memory phenotype with a restricted repertoire and are devoid of T-cell receptor excision circles.These features suggest that T-cell reconstitution has occurred predominantly through expansion of the donors' mature T-cell pool. Although restricted, their immune systems are capable of providing substantial protection to infection and respond to vaccines. We conclude that bone marrow transplant achieves long-lived reconstitution of immune function in complete DGS and is a good alternative to thymic transplantation in patients with a suitable donor.Bone marrow transplant in complete DGS using an HLA-matched sibling donor provides long-lasting immunity and is a suitable and more available alternative to thymic transplantation.
View details for DOI 10.1016/j.jaci.2007.08.048
View details for Web of Science ID 000250157700027
View details for PubMedID 17931564
Genome-wide testing: Genomic medicine
2006; 60 (3): 243-244
View details for DOI 10.1203/01.pdr.0000233116.85413.cd
View details for Web of Science ID 000239861300001
View details for PubMedID 16923947
Two-tiered universal newborn screening strategy for severe combined immunodeficiency
2005; 86 (4): 427-430
Outcomes for infants with severe combined immunodeficiency (SCID) would be improved by universal newborn screening, but there are not yet screening tests of sufficient accuracy for the disorder. In a pilot study, we assessed the ability of a two-tiered strategy to improve accuracy. Dried blood samples from patients were assessed with two tests for lymphopenia: interleukin-7, a T-cell growth cytokine, and TRECs, a byproduct of T-cell receptor recombination. IL-7 screening has a specificity of 96.1% and TRECs have a specificity of 92.3%. Combining these tests in a two-tiered strategy increases specificity to 100% (97-100% CI). Sensitivity was 85% for IL-7 screening and 100% for TREC screening. A two-tiered strategy may be of sufficient accuracy to enable universal SCID screening, and should be assessed in a prospective trial.
View details for DOI 10.1016/j.ymgme.2005.09.005
View details for Web of Science ID 000234282500002
View details for PubMedID 16260163
Potential costs and benefits of newborn screening for severe combined immunodeficiency
2005; 147 (5): 603-608
Severe combined immunodeficiency (SCID) is a rare, treatable disorder of the immune system. The incidence is unknown but may be more common than published estimates because infants frequently die of infection before diagnosis. SCID is a candidate for universal newborn screening, so there is a need to determine under which circumstances screening would be cost-effective.We assumed a screening program for SCID would use T-cell lymphopenia as the screening criterion and performed a cost-utility analysis comparing universal screening with screening only those with a family history of SCID.Assuming society is willing to pay $50,000 for every quality-adjusted life-year saved, a SCID screening test that cost less than $5 with a false-negative rate of 0.9% and a false-positive rate of 0.4% would be considered cost-effective. A nationwide screening program would cost an additional $23.9 million per year for screening costs but would result in 760 years of life saved per year of screening. The cost to detect 1 case of SCID would be $485,000.SCID screening could result in a large benefit to detected individuals, making screening relatively cost-effective in spite of the low incidence of the disease. However, an adequate test is critical to cost-effectiveness.
View details for DOI 10.1016/j.jpeds.2005.06.001
View details for Web of Science ID 000233500000012
View details for PubMedID 16291349
Persistent parvovirus-associated chronic fatigue treated with high dose intravenous immunoglobulin
2005; 24 (3): 272-274
We report a 16-year-old boy with no evidence of immunodeficiency who had a 2-year history of chronic fatigue, low grade fever and slapped-cheek rash associated with chronic parvovirus B19 viremia. Prolonged intravenous immunoglobulin therapy resulted in resolution of his symptoms and viremia. Intravenous immunoglobulin may be useful in the resolution of parvovirus viremia regardless of immune status.
View details for DOI 10.1097/01.inf.0000155194.66797.20
View details for Web of Science ID 000227567700017
View details for PubMedID 15750469
Childhood posttraumatic stress disorder and efforts to cope after Hurricane Floyd
2002; 28 (2): 61-71
The authors report on the level of posttraumatic stress disorder (PTSD) experienced by fourth-grade children 6 months after Hurricane Floyd and describe the children's efforts to cope with their stress. All of the children they studied were directly affected by the hurricane, secondary to the destruction of their school by floodwaters. The homes of 37% of these children were also flooded. Ninety-five percent of the children experienced at least mild symptoms of PTSD, and 71% had symptoms that were moderate to very severe. Children who reported that their homes were flooded were 3 times more likely to report symptoms than those whose homes were not flooded, and the girls were twice as likely as the boys to report symptoms. The high PTSD prevalence rates are comparable to findings from other studies involving violence in which 94% of the victims reported experiencing symptoms. For further analyses, the authors used symptom clusters of hyperarousal, numbing/avoidance, and reexperiencing symptoms.
View details for Web of Science ID 000180870600003
View details for PubMedID 12613287
Role of nonspecific cross-reacting antigen, a CD66 cluster antigen, in activation of human granulocytes
1996; 64 (11): 4574-4579
Nonspecific cross-reacting antigen (NCA) is the name of a family of highly glycosylated bacterial-binding receptors found on human granulocytes and other tissues. These glycoproteins are members of the immunoglobulin supergene family and are related structurally to carcinoembryonic antigen. In this study, we demonstrate that ligation of granulocyte NCA results in the activation of the cells, as measured by degranulation and the flux of intracellular calcium. These studies further the proposition that NCA has a function in the immune response of granulocytes against bacterial infections.
View details for Web of Science ID A1996VP42800024
View details for PubMedID 8890209
1994; 69 (1): 25-30
The organic solvent octane has been used routinely to permeabilize the hydrophobic vitelline membrane surrounding the Drosophila embryo, thereby allowing the movement of small molecules into the egg. We present evidence that hexane is a more effective permeabilizing agent than octane and compare the effects of these solvents on uniformity of permeabilization and embryonic viability. The ability of each solvent to make the embryo accessible to a range of biological stains was compared. The effect of octane versus hexane permeabilization on subsequent embryonic viability was measured at seven different stages during early embryogenesis. We found that although hexane is a superior solvent for permeabilizing the vitelline membrane, it decreases the viability of embryos exposed between 0 and 3 hr of age. Older embryos treated with either hexane or octane are usually viable. We also showed that molecules with a molecular mass of 984 Daltons or more did not diffuse into the embryo following treatment with either hexane or octane. Results presented here challenge a phase-partition model that has been proposed previously to explain the molecular basis of permeabilization of the Drosophila egg. An alternative model is described as well as an optimized protocol for permeabilizing and staining Drosophila embryos at any stage during early embryogenesis while maintaining viability for subsequent culture.
View details for Web of Science ID A1994MU12300004
View details for PubMedID 7511938


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Kyle Hollingsworth Band Tour Dates
September 8 Beachland Ballroom - Cleveland, OH (with The Macpodz)
September 9 The Werk Out Music & Arts Festival - Bellefontaine, OH
September 10 Bell's Eccentric Cafe - Kalamazoo, MI (with The Macpodz)
September 11 Shoe Fest - Earlville, IL
Kyle Hollingsworth Band Tour Dates :: Kyle Hollingsworth Band News