text:
Emirates Team New Zealand competeing in day one of the Extreme Sailing Series regatta being sailed in Singapore. 20/2/2014
Emirates Team New Zealand finished the first day of the Extreme Sailing Singapore regatta fourth overall.
With two wins in the eight races sailed, Dean Barker and crew (Glenn Ashby, James Dagg, Jeremy Lomas and Edwin Delaat) are well placed in the 12-boat fleet.
Sitting on 46 points, they are only two shy of third and second placed teams and 10 from the leader. They started and finished the day with a win.
Fortunes fluctuated for every team in the very shifty breeze that was a light four to 10 knots.
Barker: 'We had good results in the first few races, then faded a little finishing towards the back of the fleet and returned to win the last race.'
'After the tough racing on practice day we knew we were in for a battle this week. We have to work on consistency and eliminating mistakes.'
Emirates Team New Zealand leads in race one of the Extreme Sailing Series regatta being sailed in Singapore. 20/2/2014
Extreme Sailing Series™ 2014 Act 1, Singapore
Standings after Day 1, 8 races
1st Realstone (SUI) 56 points.
2nd Alinghi (SUI) 48 points.
3rd Groupama sailing team (FRA) 48 points.
4th Emirates Team New Zealand (NZL) 46 points.
5th J.P. Morgan BAR (GBR) 44 points.
6th Red Bull Sailing Team (AUT) 43 points.
7th SAP Extreme Sailing Team (DEN) 39 points.
8th Oman Air (OMA) 38 points.
9th The Wave, Muscat (OMA) 29 points.
10th Gazprom Team Russia (RUS) 28 points.
11th GAC Pindar (AUS) 20 points.
12th Team Aberdeen Singapore (SIN) 17 points


text:
Woman bloodies man's nose at party, man hits her back
Cause if you kiss me then I'll kiss you back
A woman is being ejected from a party, says "what are you going to do, hit a girl?" She then hits a man in the face.
After a brief pause, he hits her back, engendering a long debate on the ethics of hitting a girl who hit you first.
tags: video
Recent Comments »
11/24/13 3:41 PM by Mihow
It's better when you're here I'mAbout to eat onion rings !!!
11/24/13 3:40 PM by Mihow
lol @ ResFantastic
11/24/13 3:39 PM by Res ipsa loquitar
and LOL at nobody cares about your stats...proceeds to gives his stats. HAhHAHAHAHAH. you truly are a dim one.
11/24/13 3:35 PM by Res ipsa loquitar
11/24/13 3:29 PM by role model
First off, nobody cares who you are, or your height, weight, or body fat. Thats not what any of this is about. Im bigger than you and ive wrestled, fought, and trained one way or another my whole life, and apparently im a lot meaner than you to boot. Dont start with the tough guy talk.Second"I dont need to punch a 5'6 girl to make a point."Punching someone isnt about making a point. Jesus fucking christ. Punching someone in the face isnt a metaphorical message, Socrates.The guy who punched the cunt didnt do it to prove he was tough, or any of thaf nonsensical shit youre babbling about. Its not like it was done to prove his manhood. Its to cause someone pain, and in this case, its just a retaliation for unprovoked violence. I dont give a fuck if youre the queen of england, you dont physically assault someone and not expect it back. Thats the only thing that matters in this conversation.
11/24/13 3:22 PM by VinegarStrokes
Ya she was hiding behind her vagina which by making a quick judgement based off the video title and her fashion sense is something she only does in a situation like that.
11/24/13 3:17 PM by VinegarStrokes
Don't throw punches at a man if you can't accept the consequences. However, he waited till she was turned and walking away that was a bitch move.
11/24/13 3:10 PM by Ronda Winter
strange behavior of both
11/24/13 3:05 PM by Imploze
I have enjoyed reading this thread. I'm glad he sit the dyke down. It's obvious some if you haven't encountered a woman like her that will say, and believe, they can beat a mans ass. While I may have just slapped the taste out of her mouth, I don't fault him for the closed fist.Hopefully the young lady learned a lesson that night, and won't go around hitting people that she doesn't agree with anymore.

subset: commoncrawl


text:
- Color: Black-painted finishing
- Pieces: 4PCS
- Application: Standard expansion slots
- Length: 120mm
- Venting Dimensions: 890mm²
- Venting Percentage: 70%
- Net Weight: 8g
Works as intended 01/23/2014
This review is from: Silverstone AEROSLOTS-BP Maximum Vented PCI Slot Covers - Black Painted Finishing
The vented slot covers work exactly as intended. Actually seems to be helping the idle temp of my GPU.
Wish the price was a couple bucks lower, but I'm still happy with the product.
Other Thoughts:
Saw a couple people mention that the metal is easily bent. I didn't see this as much of a con, as it actually made it a little easier to get the last one in underneath my GPU.
Did you find this review helpful? Yes / No Thanks for the valuable feedback you provided!


text:
Republicans need all the votes they can get next November if they are to have any hope of retaining the White House and winning back control of Congress. But one group of voters -- among whom the GOP has gained considerable ground over the last few elections -- now seems about to slip away, perhaps permanently.
Hispanic voters are poised to turn several red states blue come 2008, virtually guaranteeing a Democratic presidential victory and a pickup in congressional seats as well, according to a new analysis of Hispanic voting behavior. "Border Wars: The Impact of Immigration on the Latino Vote," released by the conservative group Americas Majority Foundation, demonstrates that congressional Republicans' ham-handed approach to immigration will cost them dearly at the polls.
Richard Nadler, the study's author, analyzed 2006 voting patterns in 145 Hispanic precincts or voting blocs in three congressional districts in the Southwest. In each district, candidates who favored an enforcement-only approach faced opponents who favored comprehensive immigration reform, including a guest worker program and a path to legalization for illegal aliens. Nadler found that the enforcement-only approach significantly eroded support for the Republican candidates among Hispanic voters from their 2004 levels, and in each case the Republican lost the seat.
In Arizona's 5th district, six-term GOP incumbent Rep. J.D. Hayworth actually switched positions on immigration from being a sponsor of guest worker legislation to becoming one of the most outspoken opponents of immigration, even advocating a moratorium on legal immigration from Mexico. In 2004, Hayworth received almost as many Hispanic votes as his Democrat opponent, with each receiving about 48 percent; but in 2006, Hayworth lost 59-36 percent in Hispanic precincts.
In the 8th district of Arizona, a pro-comprehensive reform Republican, incumbent Jim Kolbe, retired, and the Republican nominee, Randy Graf, made illegal immigration the centerpiece of his campaign. In 2004, Rep. Kolbe won almost 43 percent of the vote in Hispanic precincts. In 2006, Graf won barely 18 percent of those precincts' votes -- and Republicans lost the election 54-42 percent.
Linda Chavez is chairman of the Center for Equal Opportunity and author of Betrayal: How Union Bosses Shake Down Their Members and Corrupt American Politics .
Be the first to read Linda Chavez's column. Sign up today and receive Townhall.com delivered each morning to your inbox.


text:
Summit SPR601OS $1,500.99
Summit 5.5 Cu Ft Outdoor Glass Door Refrigerator
5.5 Cu Ft Outdoor Glass Door RefrigeratorModel:SPR601OS
Sorry, the manufacturer is no longer producing this product.
Description & Highlights
Summit Professional Outdoor Glass Door Refrigerator with Reversible Door
The Summit Professional Glass Door Outdoor Fridge (model: SPR601OS) features a reversible door, stainless steel cabinet finish and front ventilation system to allow for both undercounter or freestanding use. Made of 304 grade stainless steel, this unit is designed to take on the outdoor elements and be asthetically pleasing as well. The Summit Professional Glass Door Outdoor Fridge also has the industry's largest shelves for this class of refrigerator in addition to an interior light, adjustable thermostat, front mounted lock and automatic defrost. Display your beverages with class in the Summit Professional Glass Door Outdoor Fridge with Reversible Door!
- U.L. Approved for outdoor use100% CFC Free
- Automatic defrostFan cooled compressor
- Factory Reversible doorWrapped stainless steel body
- Glass doorStainless steel towel bar handle
- Front lockInterior light
- Adjustable shelvesElectrical: 120W / 115V / 2.6A
- Adjustable thermostatWeight: 120 pounds
- Warranty: 1 year parts and labor/5 years on compressorDimensions: 33 1/8"h x 23 3/4"w x 25 1/2"d
- Height: 33 1/8"
- Width: 23 3/4"
- Depth: 25 1/2"
Owners Manuals
You may download a product owner's manual below:
**Please note, Adobe Acrobat Reader is required to view pdf files


text:
Welcome to WebmasterWorld Guest from 22.214.171.124
Forum Moderators: open
All didn't work for me, but others may have a different opinion.
are marginal to ok. The narrower the niche, the better the results. You also have to consider the traffic partners. The three that I stated to stay away from partner with anyone who has a site. Just keep that in mind.
Many of the publishers on the networks of some 2nd/3rd tier will deliver decent traffic but there will also be rogues.
To consider (depending on sector) :
Looksmart Looklistings
Enhance-Interactive (fomerly ah-ha)
Lycos Insite
Outside of those we've not had a positive experience. In some cases the rewards were not worth the effort (poor traffic quality in some cases, no traffic in others), they didn't work for certain sectors due to the demographics of the desired end-user.
The other thing to consider is the expatriated potential US clients. That brings into play the likes of Espotting, Mirago, Webfinder, Overture UK.
There are plenty of searches with a US focus where the searcher happens to be in the UK or Europe on business but is looking for fulfilment in the US.
Certainly not to be overlooked.
I'd say I've had a positive experience in some industries with all the companies above epilot. Of course in some industries I have bad experiences with all or some of these companies (including google and overture)
Google AdWords
Overture US & UK
That's all. I've got friends who have used the smaller ones like ah-ha, and most were what looked like cheat clicks. I'm not saying all the clicks are like that, but of the 6 people I know that have used smaller engines, 2 of them demanded refunds and the rest never used them again. Plus, do you even know people who use the smaller engines? Just think to yourself when determining which PPC engines to use "Who do I know that uses these engines?". Should solve it fairly quickly. Goodluck.


text:
Welcome to the historic Village of Trunch, North Norfolk
The North Norfolk historic village of Trunch with a population of approximately 1,000 lies only two miles from the East coast, close to the coastal resort of Mundesley. Our nearest town inland is North Walsham, and the historic and fine city of Norwich is around 17 miles south of Trunch.
Special Events
* Autumn Show, Garden Society
* Open Gardens & ScareCrow Festival
* Christmas Fayre 26.11.
Groveland Taster DaySaturday 28 November, 10am-4pm: If you would like to try and buy Trunch Cider, please visit our stall at the coming Groveland Farmshop (Roughton) ...
Trunch Open Gardens & Scarecrows, July 2015Again this was a great and very successful event, with many attractions, lots of happy visitors and beautiful sunshine!
The picturesque village centre also features the fine medieval Church of St Botolph (see the new guidebook) and typical old Norfolk flintstone houses within a designated conservation area. Trunch is surrounded by the North Norfolk protected area of outstanding natural beauty, and there are many public footpaths and quiet lanes, very inviting for (dog) walkers, bird watchers and conservationists.
Trunch offers a lot to residents and visitors. The village shop Corner Store also hosts a post office. You can spend a nice evening at the Crown Inn, our village pub, which offers excellent beers and nice food. Alternatively, the Social Club also offers drinks and often Live Music. Lots of exciting special events like the annual Trunch Open Gardens & Scarecrow Festival, the monthly concerts in St Botolph's church or the unique Trunchonbury Festival 2014 attract many visitors, and regular activities such as meetings of Trunch Friends or the Trunch Art Group take place in the spacious and welcoming Trunch Village Hall.Trunch Village Society has various groups and activities under its umbrella, such as the Composting Group, the Cider Coop , the Beekeeping Group and the Art Group, and organises the annual Trunch Open Gardens & Scarecrow Festival.
Trunch website is a work in progress. If you want to advertise here, publish an event or have a story about Trunch to tell, please contact us. Co-editors are always welcome.


text:
Next-generation sequencing methods have contributed to rapid progress in the fields of genomics and population genetics. Using this high-throughput and cost-effective technology, a number of studies have estimated single nucleotide polymorphism (SNP) frequency by calculating the mean number of SNPs per unit sequence length (e.g., mean SNPs/kb). However, both read length and contig depth are highly variable and thus raise doubt about simple methods of SNP frequency estimation.
We used 454 pyrosequencing to identify 2,980 putative SNPs in the eastern tiger salamander (Ambystoma tigrinum tigrinum) transcriptome, then constructed analytical models to estimate SNP frequency. The model which considered only contig length (i.e., the method employed in most published papers) was evaluated with very poor likelihood. Our most robust model considered read depth as well as contig length, and was 7.5 × 1055 times more likely than the length-only model. Using this novel modeling approach, we estimated SNP frequency in protein-coding (mRNA) and non-coding transcripts (e.g., small RNAs). We found little difference in SNP frequency in the contigs, but we found a trend of a higher frequency of SNPs in long contigs representing non-coding transcripts relative to protein-coding transcripts. These results support the hypothesis that long non-coding transcripts are less conserved than long protein-coding transcripts.
A modeling approach (i.e., using multiple model construction and model selection approaches) can be a powerful tool for identifying selection on specific functional sequence groups by comparing the frequency and distribution of polymorphisms.
Keywords:Contig depth; Contig length; Model selection; SNP frequency; Transcriptome; 454 sequencing
Mutations and subsequent polymorphisms are important not only for identifying influences on genetic disease [1,2], but also for understanding selection, adaptation, and other evolutionary processes [3,4]. While some new mutants have higher fitness than others and therefore are retained by positive selection, most mutations are quickly removed by purifying selection because they are deleterious [5,6]. The intensity of selection may differ across genomic locations (e.g., genic and intergenic regions) and thus nucleotide polymorphisms have different evolutionary impacts depending on their genomic locations. Single nucleotide polymorphism (SNP) profiles can be used to infer the intensity of selection by comparing the frequency and distribution of SNPs of two or more sequence regions, such as genic vs. intergenic regions, exonic vs. intronic vs. untranslated regions, autosomes vs. sex chromosomes, and particular gene groups with specific functions [7-9].
To properly compare SNP frequency among groups (e.g., among functional gene groups, among chromosomes, among populations), large samples of similar size are desirable. For example, the International HapMap Consortium [10,11] estimated the frequency and distribution of human SNPs at the genome-wide level; they compared these among three geographically diverse populations by genotyping 90 individuals from each population. To date, such large-scale, systematic evaluations have been limited to human or a few model species e.g., [12].
The advent of high-throughput sequencing technologies (e.g., 454, SOLiD, and Illumina) has contributed to the rapid development of SNPs in non-model but important wild species [7,9,13,14]. In the context of next-generation sequencing, SNP discovery in non-model species is usually achieved by comparing the consensus sequence of a contig with the individual sequences that comprise the contig. However, it is not easy to estimate the frequency of such SNPs because of extreme variation in contig read length and depth; we are more likely to observe SNPs in a 10 kb-long-sequence than in a 1 kb-long-sequence. Furthermore, we have a higher probability of identifying SNPs when we compare contigs comprised of 100 reads than with those comprised of 2 sequence reads. Despite these multiple factors, most next-gen analyses estimate SNP frequency simply by determining the average number of SNPs per unit sequence length (e.g., mean SNP/kb), thus ignoring bias due to contig depth. One of our goals was to determine if such length-only-methods can effectively estimate SNP frequency from next-gen datasets or if more sophisticated approaches are needed.
A second goal was to compare SNP frequencies in protein-coding transcripts vs. non-coding transcripts. A "transcriptome" consists of transcripts not only from protein-coding mRNA but also from non-coding RNAs such as ribosomal RNA (rRNA) and transfer RNA (tRNA). In general, non-coding transcripts are expected to be less conserved (i.e., more polymorphic) than protein-coding regions because selection may eliminate deleterious mutations from functional sequences [15,16]. However, recent transcriptome studies have shown that there are many types of non-protein-coding RNAs with functions of biological significance such as chromatin modification and transcriptional regulation [17]. This may imply that at least some non-coding transcripts are as well conserved as protein-coding transcripts. However, (to our knowledge) no study has systematically evaluated SNP frequencies in coding and non-coding transcripts at the transcriptome level. Although there are some reports that long non-protein-coding RNAs in humans and mice have evolved rapidly [18-20], the data are still lacking—especially in non-mammalian species.
One of our ongoing research programs is focused on evolutionary and ecological genomics of tiger salamanders [21-24]. For this research, we sequenced the transcriptome of the eastern tiger salamander (A. t. tigrinum) using 454 pyrosequencing and then analyzed the distribution and frequency of SNPs. We studied the influence of contig depth on the estimation of SNP frequencies, and we tested the hypothesis that non-coding transcripts are less conserved than protein-coding transcripts by comparing their SNP frequencies.
Sample preparation and 454 transcriptome sequencing
We captured and sampled seven tiger salamanders at the Purdue Wildlife Area (Indiana, USA) consistent with IACUC 1203000614 and in accordance with guidelines by the American Society of Ichthyologists and Herpetologists and by the American Veterinary Medical Association (June 2007). RNA was extracted from gill, lung, skin and spleen using TRIzol® reagent (Invitrogen). We constructed a total of 19 tissue-specific cDNA libraries from these RNA samples using the ClonTech SMART cDNA synthesis kit, utilizing a modified poly-T primer [25]. Each double-stranded cDNA library was digested with SfiI to remove excess primers and subsequently purified with the QIAquick PCR purification kit (Qiagen). Approximately 5 μg of each cDNA library was sequenced using a 454 Genome Sequencer with FLX Titanium chemistry (454 Life Sciences). Briefly, the cDNA fragments were sheared via nebulization, hybridized to DNA capture beads, and amplified by emulsion-based PCR. Molecular barcodes with multiplex identifiers (MIDs, unique 10 bp sequences) were applied to specifically tag each cDNA library in our pooled sequencing run.
Contig assembly and SNP detection
We pooled all sequences from seven individuals in PCAP [26] and assembled contigs from the entire data set using default settings with the overlap cutoff of 92% identity. The consensus sequence from each of these contigs was used as a reference sequence to which individual sequence reads were aligned using PCAP. First, we identified SNPs only from contigs at least 100 bp in length and with a depth of 10 or more reads. We limited SNP scoring to contigs which have minor allele frequency of 0.01 or at least two minor allele reads. Furthermore, we limited SNPs to regions where 20 bp of high-quality sequence data was present both upstream and downstream of the variable site. We discounted SNPs in homopolymer repeats of > 4 nucleotides, and defined high-quality sites as those with a PCAP base quality score >20. We considered only biallelic SNP substitutions, excluding all indels and triallelic sites. Second (in a separate analysis), we identified SNPs in long contigs (at least 501 bp) [27] using otherwise identical methods. These long contigs are thus a subset of all contigs. Our use of these two datasets ("all" contigs of ≥100 bp and "long" contigs of >500 bp) allowed us to evaluate SNP frequencies in long transcripts.
Discriminating among protein-coding and non-protein-coding transcripts
We divided all contigs into categories of protein-coding and non-coding transcripts using the program CPC [28]. CPC employs supervised learning algorithms known as support vector machines to discriminate between protein-coding and non-protein-coding transcripts [28,29]. The program assessed the protein-coding potential scores of each contig based on the quality of the predicted open reading frame (ORF) and on the quality of the BLAST hit against UniProt Reference Clusters. CPC was used to determine ORF quality by log-odds score, coverage, and integrity of the ORF whereas BLAST hit quality was determined by BLAST hit number, feature of high-scoring segment pairs (HSP), and HSP frame score among three reading frames. Positive coding potential values reflect protein-coding transcripts whereas negative coding potential values designate non-protein-coding transcripts. Thus, we distinguished protein-coding from non-coding transcripts by considering coding potential scores of a contig (and by checking the score of its reverse complement strand).
Modeling SNP frequencies and comparisons between protein-coding and non-coding transcripts
Our first goal was to evaluate the effects of contig length and depth on the estimated frequency of SNPs. A priori, we predicted that SNP frequency would positively associate with both factors. We employed the information-theoretic approach suggested by Akaike [30] and extended by Burnham and Anderson [31] to evaluate the relative plausibility of models relating contig length and depth to the number of SNPs in contigs. Our second goal was to test the hypothesis that SNP frequency in non-coding transcripts is higher than in protein-coding transcripts. To test this, we included transcript type (protein-coding or non-coding) as a variable in all models. Therefore, the global model consisted of length (LENGTH, the number of bp), depth (DEPTH, the number of aligned sequence reads), and the variable of transcript type (C/NC, coded with 1 for protein-coding transcript and 0 for non-coding transcript) of each contig. We used SAS 9.2 to estimate parameter values.
Four candidate models were fitted using regression models based on a negative binomial distribution. To assess the relative fit of each model, we compared the Akaike's Information Criteria (AIC), with better fitting models having lower AIC [31]. The relative plausibility or weight of each model was evaluated by measuring Akaike weight (wi). We repeated construction of candidate models and model selection for the number of transitions (Ti) and transversions (Tv) as the response variables, as well as for the number of SNPs in a contig. For ease of interpretation of the relative effect of each variable, we plotted estimates of the relationships between length or depth of contigs and the number of SNPs found in protein-coding and non-coding transcripts.
Our 454 pyrosequencing run on the cDNA from seven tiger salamanders yielded a total of 670,408 sequence reads spanning 190.4 Mb. After quality control and other filtering (e.g., repetitive elements, short reads, etc.) we assembled the remaining 273,501 high-quality sequences into 51,391 contigs. Among contigs with at least 10 sequence reads, 4,552 and 3,181 contigs were ≥100 and ≥501 bp in length, respectively (Table 1; Additional file 1). In the "all" contig dataset of ≥100-bp contigs, protein-coding transcripts were slightly more rare than non-coding transcripts (2,146 vs. 2,406); the pattern was reversed in the "long" dataset of ≥501-bp contigs where protein-coding transcripts were more common (1,762 vs. 1,419). A total of 2,980 SNPs that passed our stringent scoring criteria were detected in the "all" contig dataset; of these, 2,515 (84%) were found in the "long" dataset (Additional file 1). The ratio Ti/Tv was 1.8 in both datasets. An overview of the assembled contigs and SNPs is presented in Table 1 and our short read sequence data will be deposited at Dryad (http://dx.doi.org/10.5061/dryad.5pd17).
Additional file 1. Contigs and SNPs. Information on 4,552 contigs with ≥10 sequence reads and ≥100 bp in length, and 2,980 SNPs found in the contigs.
Format: XLSX Size: 379KB Download file
Table 1. Summary statistics of assembled contigs (with depth of ≥10 reads) and SNPs
The correlation results were qualitatively similar between the "all" (Additional file 2: Figure S1) and "long" datasets (Figure 1). Here we focus on the long dataset, where the number of SNPs observed in a contig averaged 0.79 and ranged from 0 to 26. Mean contig length in the long dataset was 1,003 bp (range 501 – 5,354 bp) and mean contig depth was 32 reads (range 10–315). We found positive associations between the number of SNPs and contig length (r = 0.32; P < 0.001; Figure 1A), and between the number of SNPs and contig depth (r = 0.46; P < 0.001; Figure 1B). These same trends were apparent in both protein-coding (r = 0.36 for length vs. SNP frequency; r = 0.47 for depth vs. SNP frequency) and non-coding transcripts (r = 0.22 for length vs. SNP frequency; r = 0.44 for depth vs. SNP frequency); all of these associations were significant (P < 0.001).
Additional file 2. Supporting figures and tables. A file containing additional data: Figure S1; Figure S2; Table S1; and Table S2.
Format: PDF Size: 1003KB Download file
This file can be viewed with: Adobe Acrobat Reader
Figure 1. Correlations between the number of SNPs and both length and depth of contigs. Positive correlations between the number of SNPs and both (A) length and (B) depth of contigs with depth of ≥10 reads and length of ≥501 bp. In both cases, P < 0.001.
To estimate SNP frequency in the 'long' (≥ 501 bp and ≥10 reads) contig dataset, we used the negative binomial distribution to construct four regression models that considered contig length, contig depth, and the category of transcript (protein-coding and non-coding transcript). The most robust model of SNP frequency was the global model that used all of these variables; similarly, the global models were most robust for estimating the frequency of transitions (Ti) and transversions (Tv) (Table 2). The wi (relative plausability or weight of each model) of the global models for estimating the frequency of SNPs, Ti, and Tv, ranged from 0.68 to 0.99. In sharp contrast, those of the commonly-employed "length-but-no-depth" models ranged from 6.7 × 10-24 to 1.2 × 10-56. Our best-fit model predicting SNP frequency was 7.5 × 1055 (= 0.904/(1.2 × 10-56)) times more likely than the length-but-no-depth model (Table 2). Note the depth-but-no-length was also a much better model than the commonly employed length-but-no-depth model (Table 2). The "all" contig dataset yielded qualitatively similar results (Additional file 2: Table S1).
Table 2. Model selection among candidate models predicting frequencies of SNPs, transition and transversion in contigs
Estimation based on the best-fit model (Table 3) indicated that SNP frequency increased with contig length and depth in both protein-coding and non-coding transcripts. Using the long dataset, we estimated a marginally higher frequency of SNPs in non-coding transcripts than in protein-coding transcripts (C/NC coefficient of −0.09; P = 0.08) but this trend was not apparent in the "all" contig dataset (C/NC coefficient of 0.04; P = 0.43; Additional file 2: Table S2; Additional file 2: Figure S2). From this model, we estimated the number of SNPs in the long dataset as 0.48 (protein-coding) and 0.52 (non-coding) in 1 kb-long contigs with a coverage depth of 10 reads, respectively (Figure 2). Similarly, we predicted 1.78 (protein-coding) and 1.97 (non-coding) SNPs in 1-kb long contigs with a depth of 100 reads, respectively.
Table 3. Parameter estimates from best fitting model predicting frequencies of SNPs, transition and transversions in contigs
Figure 2. Estimates of the number of SNPs in transcripts based on length and depth of contigs. Estimates and comparison of the number of SNPs in protein-coding and non-coding transcripts based on various length and depth of contigs (depth of ≥10 reads; length of ≥501 bp), using best fitting models (Table 3).
We used 454 pyrosequencing to generate 190 Mbp of tiger salamander transcriptome sequences that contained 2,980 putative SNPs. These high quality candidate SNPs, identified using strict criteria, can be converted into genetic markers for studies of natural selection, genomic organization, and allele-specific expression [14,32-34]. We used these data to develop new models for the estimation of SNP, Ti, and Tv frequency; we then compared these estimates in coding and non-coding transcripts as discussed below.
Modeling frequency estimates of SNP, Ti, and Tv
A number of recent studies have estimated SNP frequency using high-throughput sequencing technology by calculating the mean number of SNPs per unit sequence length. For example, Vera et al.[14] estimated a frequency of 6.7 SNPs/kb in butterflies, Külheim et al.[9] estimated 27.1 and 38.3 SNPs/kb in exons and introns, respectively, in eucalyptus, and Renaut et al.[7] found SNP densities of >20 SNPs/kb in genes for DNA transposition, mitotic spindle organization, and biogenesis. Such findings have provided broad perspective on SNP distribution across diverse species, but all of these studies considered only the effect of sequence length variation; i.e., they estimated SNP frequencies per unit length without regard to the effect of variation in contig depth. Not unexpectedly, our data show that SNP frequency estimates are strongly and positively associated with contig length and with contig depth. Furthermore, our analyses suggest that polymorphism frequency estimates based on contig length-but-not-depth produce biased results; SNPs, Ti, and Tv were more abundant in longer contigs assembled with more sequence reads. This finding suggests that both length and depth need to be considered for estimating the frequency of polymorphisms from next-generation sequence data.
A multi-model inference framework is informative for comparing the relative importance of the variables in our models. Our most robust model of frequency estimation was the global model that included contig length, depth, and transcript type. Importantly, contig depth is a more important variable than contig length when estimating SNP frequencies. This is clear from the Akaike weight (wi) of the models, because wi is best interpreted as the relative likelihood or probability of the model [31]. Consider that our global model of SNP frequency estimation was only 9.4 times more likely than the next best model (contig depth and transcript type, but no length). In stark contrast, the global model was 7.5 × 1055 times more likely than the model of length and transcript type (i.e., no depth; Table 2). In the context of massively parallel sequencing, this suggests that SNP frequency estimation by simply correcting for contig length is inadequate. Our results support the idea that SNP frequency increases with contig length and depth, but that contig depth is a much more meaningful factor in SNP discovery.
Comparison of SNP frequency between protein-coding and non-coding transcripts
Our modeling approach can be used as a tool for inferring the intensity of selection on different categories of genes or genomic regions by comparing the SNP frequency among categories. Similar approaches have been developed for nucleotide diversity parameters such as beta (β) [35]. We studied the distribution of SNPs with regard to the protein-coding status of an expressed transcript, but our approach should prove informative in other comparisons across genomic categories (e.g., exons versus introns, class I versus class II transposable elements, etc.).
In the current analyses, we included the dummy variable C/NC in all candidate models to test for differences in SNP frequencies between protein-coding and non-coding transcripts. In the long dataset with 10 or more reads we found a negative coefficient of C/NC, implying SNP frequency in non-coding transcripts is higher than that in protein-coding transcripts. Our use of a poly-dT primer for cDNA synthesis may have biased our representation of the transcriptome, but a similar pattern has been reported in human genomic DNA. Zhao et al.[8] investigated the distribution of human SNPs and found that SNP frequency was higher in intronic or intergenic than in exonic regions. Our results support the hypothesis that reduced SNP frequency in protein-coding regions relative to non-protein-coding transcripts can be attributed to the selection pressure on functionally important protein-coding regions [15,16]. However, absolute SNP frequency values we report here should be viewed with some caution because our transcripts were sequenced from a pool of seven tiger salamanders and pooled contig depth does not directly correspond to the actual number of haplotypes [35]. Although we cannot estimate standard nucleotide diversity parameters such as theta (θ) [36] from pooled transcripts from multiple individuals, SNP frequency by our modeling approach is still useful as a relative estimate to compare the selection pressure on different transcripts [35,37].
Our data indicate there is considerable variation in SNP frequency across transcribed sequences in A. t. tigrinum. These results are consistent with the observation that different classes of functional, non-coding RNAs (e.g., microRNAs) evolve at different rates in humans and mice, with faster evolution in longer functional non-coding RNAs [18]. That said, our observed SNP frequency differences between coding and non-coding transcripts were subtle. There are several possible explanations for this. First, we relied on the CPC program [28] to parse protein-coding and non-protein-coding transcripts. CPC does so for each contig by evaluating the predicted ORF quality and the quality of the BLAST hit. This program generally performs well (accuracy of 92 ~ 96%; [28,29], but some transcripts remain ambiguous or are falsely allocated to the wrong category. In particular, categorical assignment (coding vs. non-coding) can be limited by the number of reference genomes available for comparison [29]. The tiger salamander is not a genomic model species (e.g., no complete genome sequence is available) and as of yet has no close relatives with sequenced genomes, so this complicates the identification of orthologous sequences and protein homologs used in categorical (coding/non-coding) transcript assignments.
A second source of bias may be the short read sequences generated by next generation sequencing. For example, some of our sequences may be derived from short sections of an untranslated region (e.g., 5' or 3' UTR) that occurs in a true protein-coding transcript. Such sequences will be erroneously categorized as non-coding transcripts due to poor ORF-related score, obfuscating SNP frequency differences between protein-coding and non-coding transcripts.
Finally, there may be true biological differences in SNP frequency between protein-coding and non-coding transcripts or within a transcript category—particularly when considering "long" transcripts. Recent analyses have revealed that even transcripts which do not encode proteins may have important functions and some of them are conserved to maintain functional domains and structures [17,38-40]. Many short non-coding transcripts such as microRNAs and small nucleolar RNAs are known to be highly conserved, functional non-coding RNAs across a diverse range of species [18]. In contrast, some long non-coding RNAs with a known function, such as Xist and Air, are poorly conserved across taxa [18,41]. This suggests rapid evolution of long non-coding RNAs despite their known functions (e.g., involved in X-chromosome dosage compensation for Xist and silencing an imprinted gene at the Igf2r locus for Air) [42,43]. Such biological differences between long and short non-coding RNAs might be reflected in our "all" contig and "long" contig datasets. We found no difference in SNP frequency between coding vs. non-coding transcripts when all contigs were considered, but we did observe a trend of greater SNP density in non-coding transcripts compared to coding transcripts when only long contigs were considered. This observation provides indirect evidence that "long" non-coding transcripts have relatively fast rates of molecular evolution.
Our transcriptome results revealed that estimates of polymorphism frequency are affected by both length and depth of contigs, such that simply dividing the total number of SNPs by the sequence length produces biased frequency estimates. In next-generation sequencing studies where reads vary dramatically among contigs, we propose that estimates of SNP frequency should be presented as the standardized number of SNPs for a given contig length and depth instead of the current "standard" of SNPs per unit length (i.e., SNPs/kb). For example, polymorphism frequency might be reported as the number of SNPs in a 1 kb-long transcript with coverage depth of 10 reads. Even longer and deeper contigs will become more common as sequencing technology continues to advance (so it is currently fruitless to recommend a particular read depth), but authors should at least provide readers with information about contig depth as well as length when reporting SNP frequencies.
Our modeling approach revealed that long non-coding transcripts have marginally higher SNP frequencies than protein-coding transcripts. Future models that consider more variables may further refine our ability to estimate SNP frequency, so the absolute frequency values we report herein should be viewed with some caution. Nevertheless, the relative polymorphism frequency values reported herein support the hypothesis that long non-coding transcripts are less conserved than long protein-coding transcripts, perhaps as a function of selective constraints. We think that our modeling approach (i.e., using multiple model construction and model selection approaches) can be a powerful tool for identifying selection on specific functional sequence groups by comparing the frequency and distribution of polymorphisms.
Competing interests
The authors declare no conflict of interest.
Authors' contributions
SHE and JAD conceived and designed the experiments. SHE performed the experiments and analyzed the data. JAD contributed reagents/materials/analysis tools. SHE and JAD wrote the manuscript. All authors read and approved the final manuscript.
We thank Dr. Robert K. Swihart and members of the DeWoody lab for comments on this manuscript. This research was supported by a grant from the National Science Foundation (DEB-0514815) to JAD and from University Faculty Scholar funds provided through Purdue's Office of the Provost.
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L2180 - Addition to lower extremity fracture orthosis, plastic shoe insert with ankle joints
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After President Barack Obama delivered his State of the Union address to a joint session of Congress on Tuesday night, it didn't take long for candidates running for the Republican presidential nomination to sound off on the speech.
The message conveyed in Obama's remarks is one he can be expected to reinforce in the months ahead on the campaign trail.
Below, a slideshow of what Newt Gingrich, Mitt Romney, Rick Santorum and Ron Paul had to say about this year's State of the Union speech.
(Click here for HuffPost's live blog coverage of the event. Click here for a recap and to watch highlights from the president's address.
Former House Speaker Newt Gingrich released the following statement on Obama's address. We have a crisis of work in this country and tonight President Obama proposed nothing in the way of policy changes that will get us to robust job creation and dramatic economic growth. Instead, the president described his conviction that his big government is built to last and should be paid for with higher taxes. But bigger government and higher taxes will not lead to jobs and growth. Bigger government and higher taxes will instead lead to more people on food stamps, a situation which the President and his party defend as a fair outcome. Here we have to confront the truth about President Obama. Economic growth and prosperity is not really at the top of his agenda. He will always prefer a food stamp economy to a paycheck economy and call it fair. For the president and a large part of the political class, it's about their power, their right to rule. They just want to take money from Joe the Plumber - the small business people who makes over 90 per cent of the new jobs -- and redistribute it to the government bureaucracy and their political friends and allies. That's why so much of that nearly trillion-dollar stimulus didn't create jobs but just went into the pockets of special interests who support President Obama and the leadership of the Democratic Party. No better example of this exists than in the crisis of American energy. President Obama and his political allies - not of few of whom love living in energy inefficient houses or driving gas-guzzling luxury vehicles - openly admit they want gas prices to remain high so that the rest of America will learn to live more modestly. They think it's good for rest of us. Only recently, the president canceled the Keystone XL Pipeline that would have created countless new jobs and helped America on the way to energy independence because he wanted to appease the far left of his party. And yet not a single word on the Keystone XL pipeline tonight. To create jobs and growth in this country, we must start with dramatic tax reform that lowers taxes and maximizes capital investment and job creation. We must return to a dollar as good as gold whose purchasing power is the same in thirty years as it is today. We must dramatically expand American energy production. We must have smarter regulation at the same time we abolish destructive and costly regulatory systems beginning with Obamacare,Dodd-Franks, and Sarbanes-Oxley. And finally, unlike the current administration, we must have faith in job creators. With these policies the state of the union will be much better. They will create an explosion in job creation and lead to robust economic growth and a return to prosperity. Furthermore, a paycheck economy will put us on a path to balanced budgets and paying down our national debt.
Mitt Romney gave his reaction to Obama's State of the Union address on NBC Tuesday night. Romney told Brian Williams Obama had "basically adopted a lot of thoughts that we've had on the campaign trail." He also touched on his time campaigning in Florida, noting that "the idea that we are on the right track is something very foreign to people here." Romney used the opportunity to explain that he feels capital gains taxes should be low to "encourage people to build new enterprises." "If you raise the capital gains tax rate, there will be less money going into creating jobs," Romney said. Romney also appeared on Fox News Tuesday night to discuss how he believes Obama views Americans. "I just dont think he understands what's happening in American homes," Romney said. "I just sat down with a group of Floridians... they're not feeling like the policies of this president have made things better." Romney said there was "no question" that Obama hasn't improved the country during his presidency. "This is a president with a gap between what he says and what he does so enormous, sometimes you just... shake your head in amazement." Romney questioned what Obama did during the first two years of his presidency, when he had "all that power" of a Democratic majority in the House and Senate. -- Paige Lavender
Former U.S. Senator Rick Santorum issued the following statement on the president's speech: "Tonight Barack Obama transformed the President's annual State of the Union address into the kick-off of his re-election campaign. From beginning to end, the American people heard more of the same - empty promises and grand platitudes that will do nothing to help the millions of Americans who are unemployed or under employed find a good paying job. Rather than call for decisive action in allowing projects like the Keystone Pipeline or reducing the regulatory burden his Administration has imposed, the President declared war on those who are most successful in our society. Barack Obama should realize he's the President of all Americans, but sadly, he has instead chosen to govern and campaign as the Divider-in-Chief. We need to unite America and create an environment that rewards hard work and success, allowing people the opportunity to rise in society. We need a President who will rebuild the sector that built American economic greatness - manufacturing. My plan is diametrically opposed to that of the President's. Barack Obama speaks of raising taxes and imposing barriers to growth, my plan would eliminate the corporate taxes on manufacturers, eliminate the burdensome regulations of this Administration, and free our market to explore for the energy necessary to grow our economy. Our Party needs to provide a clear contrast with Barack Obama in the general election. Our campaign does just that by focusing a positive message of a resurgent American manufacturing sector, an Administration that will believe in American exceptionalism again, and valuing the dignity of each and every human life. America deserves better than what they heard from Barack Obama tonight. "
U.S. Rep. Ron Paul issued the following statement on Obama's speech: "Tonight, President Obama once again showed that he does not represent the fundamental change this country needs. Instead of offering solutions to the problems our country faces, the President was intent on delivering a campaign speech, further dealing in the typical Washington political gamesmanship that has gotten us exactly nowhere close to improving the lives of the American people. "In a speech where much of the rhetoric was devoted to job creation, it was strange that President Obama would brag about his job-destroying national health care plan, Obamacare, and the Dodd-Frank bill, which, contrary to the President's claims, guarantees future taxpayer bailouts of large institutions. Unfortunately, President Obama's 'job creation' policies amount to little more than continuing to allow government bureaucrats to pick winners and losers, which is a recipe for continued economic stagnation. "President Obama claims to want an economy where everyone gets a fair shot, everyone does their fair share, and everyone plays by the same set of rules. Yet he remains committed to the same old system of debt, deficits, bailouts, and cronyism that created our economic problems. The President speaks of giving us energy independence from unstable nations, yet he refuses to allow the type of development needed to achieve this goal, while at the same time his administration hands out favors to the politically connected - those given to the likes of Solyndra, who fail to produce jobs or energy but succeed in ripping off the taxpayers. "Of course, President Obama refuses to even mention the role the Federal Reserve plays in creating an economic system where some are denied a fair shot or even to support my efforts at bringing transparency to the Federal Reserve. Also not mentioned by President Obama is the very crucial need for reining in spending and balancing the federal budget. What is called by some 'the greatest threat to our national security' seems not to be of great importance to this President, although I, like many Americans, believe it to be cause for immediate measures, like the $1 trillion in spending cuts that would take place in my first year as President under my Plan to Restore America. "In the area of foreign policy and civil liberties, President Obama's rhetoric may be different, but the substance of his polices - as shown by his administration's defense of the TSA's treatment of my son, Senator Rand Paul, is hardly 'change we can believe in.' No wonder more and more Americans, especially young people, are rejecting the phony alternatives of Obama and establishment Republicans and embracing my campaign to Restore America Now."
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1Therefore, as a fellow elder and a witness of the sufferings of Messiah, and also a sharer of the esteem that is to be revealed, I appeal to the elders among you:
2Shepherd the flock of Elohim which is among you, serving as overseers, not by compulsion but voluntarily, not out of greed for filthy gain, but eagerly,
3neither as being masters over those entrusted to you, but being examples to the flock.
4And when the Chief Shepherd#See 2:25. appears, you shall receive the never-fading crown of esteem.
5In the same way, you younger ones, be subject to elders. And gird yourselves with humility toward one another, for "Elohim resists the proud, but gives favour to the humble."
6Humble yourselves, then, under the mighty hand of Elohim, so that He exalts you in due time,
7casting all your worry upon Him, for He is concerned about you.
8Be sober, watch, because your adversary the devil walks about like a roaring lion, seeking someone to devour.
9Resist him, firm in the belief, knowing that the same hardships are experienced by your brotherhood in the world.
10And the Elohim of all favour, who called you to His everlasting esteem by Messiah יהושע, after you have suffered a while, Himself perfect, establish, strengthen, and settle you.
11To Him be the esteem and the might forever and ever. Amĕn.
12Through Sila the trustworthy brother, as I reckon, I have written to you briefly, encouraging and witnessing that this is the true favour of Elohim. In this stand fast.
13She who is in Baḇel, chosen together with you, greets you, also my son, Mark.
14Greet one another with a kiss of love. Peace to all of you who are in Messiah יהושע. Amĕn.
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"To find beauty in all forms is an expression of our humanity." -Deborah Stein, Designer/Director/Namer of Things, Bonbon Oiseau Designs
Artists and designers. {bonbon oiseau workshop} creates jewelry and adornment, experiences and ethereal objects since 2004. We are moving to a brighter and more beautiful space for all we have to offer you so stay tuned and follow us @bonbonoiseau on Instagram and Twitter for details and of course at Bonbon Oiseau Jewelry on Facebook too!
bonbon: {french}; sweet, a little candy, something good // oiseau (wazoe) {french}; bird


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Alternative vs. conventional treatment given on-demand for gastroesophageal reflux disease: a randomised controlled trial
1 Department of Medicine, Innlandet Hospital Trust, Gjøvik, Norway
2 Unit for Applied Clinical Research, Norwegian University of Science and Technology, Trondheim, Norway
3 Department of Medicine, Nord-Trøndelag Hospital Trust, Namsos, Norway
4 Department of Medicine, Innlandet Hospital Trust, Tynset, Norway
BMC Complementary and Alternative Medicine 2009, 9:3 doi:10.1186/1472-6882-9-3Published: 24 February 2009
Alternative treatments are commonly used for various disorders and often taken on-demand. On-demand treatment of gastroesophageal reflux disease (GERD) with pharmaceutical products is an established, cost-effective strategy. Comparisons between alternative medicine and pharmaceutical products are rare. The aim of this trial was to compare on-demand treatment with a pectin-based, raft-forming, natural, anti-reflux agent (PRA) with that of esomeprazole 20 mg (Eso20) in patients with mild/moderate GERD.
Patients with mild/moderate GERD were randomised to a six weeks' on-demand treatment with PRA or Eso20 in a pragmatic, open, multicentre trial. Overall satisfaction with treatment, satisfactory relief on a weekly basis, reflux symptoms, and treatment preferences were noted.
Seventy-seven patients were included in the analyses. Eso20 was significantly superior to PRA for proportion of overall satisfied patients (92% and 58% respectively; p = 0.001), reduction of symptoms (mean symptom scores at the end 5.9 and 8.0 respectively; p = 0.019), proportion of weeks of satisfactory relief (89% and 62% respectively; p = 0.008) and proportion preferring continuation with the same treatment (85% and 42% respectively; p < 0.001). Older patients were more satisfied than younger, and patients preferring on-demand treatment had lower symptom scores at inclusion than those preferring regular treatment.
On-demand treatment with esomeprazole 20 mg was clearly superior to the pectin-based raft-forming agent. Most patients preferred on-demand treatment to regular treatment. Those preferring regular therapy had significantly more symptoms at inclusion.
Trial registration
ClinicalTrials.gov: NCT00184522.


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Design champions 'failing to make a difference', says MP
Debra Shipley MP, Parliament's self-appointed architectural representative, has condemned the government's 'Design Champion' initiative, claiming the policy has 'failed to make any substantive difference'. Shipley said the much-heralded scheme - to ensure that construction carried out by government departments is of a high design standard - should be abandoned in its current form and reinvented at a local level.
Shipley told the AJ that many departmental champions have done 'absolutely nothing'. And those that have, 'have only focused on one or two landmark schemes'.
'I have written to all the departments to find out the progress that has been made, ' she said. 'It is very sad but there are very few positives. With the best will in the world, most of the champions are out of their depth. They have no idea about the positive impact design can have.'
She also maintained that progress such as that made by Lord Hunt in the Department of Health was only superficial. And that other champions, such as Baroness Ashton in the Department for Education and Skills, had made no progress at all.
Shipley asserted that 'major changes' are needed if 'any results are to be seen', including the introduction of design champions at the local level since ministers have failed. 'I want to see local education authorities, NHS trusts and local government all with their own champions. And rather than have it suggested that they consult CABE over big projects, it should be compulsory, ' Shipley added.
However, both the RIBA and CABE dismissed the criticism, admitting that while the scheme had been far from perfect it had had a largely positive impact. The RIBA's head of government affairs, Jonathan Labrey, said: 'It would be unrealistic to expect good design to be at the top of every agenda.
The last thing we should do is criticise them when we are really beginning to influence many of the departments.'
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is positive and released at the close of the trading session on Friday. Monday should be interesting.
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Roger Agness Model
Zack ZollPhotographer Male Glens Falls, New York, US
My Website: www.ZackZoll.comMy MM URL: http://www.modelmayhem.com/zzoll
Mayhem # 345508
About Me
I shoot both film and digital, but I heavily favour my film cameras, and shoot mostly black and white film. A lot of my shooting is with a view camera (the old thing with the heavy tripod and a cloth over my head), and that can be pretty slow going. I've had some bad experience with impatient and obnoxious escorts ruining the shoot; if you want to bring an escort, please make sure that they can entertain themselves until we're done.
Also, please don't bring an escort that is really into photography. It seems like a good idea, but if they're interested they may end up asking a bunch of questions, and that makes it harder for me to focus on you.
Right now, I'm primarily concerned with working on the two WIP projects in my portfolio. I don't have much trouble getting subjects, so I'm mostly doing TFP. But if you're willing to pose nude, and you're a man, or a woman that is curvy, non-white, or over 50, then we might be able to talk about compensation.
I will do other shoots too, but those other projects get priority.
Have you worked with Zack Zoll? Add Credits for Zack Zoll! >>
Credit Notes
Models whom I've worked with:
Kaitlyn Anne, #2116928
Lauren Price, #1566381
Lisa Delano, #2808122
Vanessa Dewar, #840733
Vyking, #2502097
Kimberly Moore, #307002
Rhea HT, #344123
Ana Ivanova, #2169531
Roger Agness Model
Pedro Trevino Photographer
johel Photographer
Jennifer Haggerty Photographer
chicagosho... Model
Author EJ Acosta Model
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text:
Sensual poetry.
The feel of silk on my skin
is like a lover's touch,
trailed oh so lightly
causing flesh to bump.
Soft as whispers in my ear
which move hairs on skin,
sending thoughts to other realms
where sanity is a sin.
Draped across my naked flesh
ignites fevered flames,
to later cover bodies
as sleep lays its claim.
Welcome to Authspot, the spot for creative writing.
Read some stories and poems, and be sure to subscribe to our feed!


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Bell Great Bridge
124 Fairwind Drive, Chesapeake, VA
Are you the manager of this property?Click here to claim this listing Check Your Credit Now
About Bell Great Bridge
Bell Great Bridge is an apartment home community located in the heart of Chesepeake, VA. We are mere minutes from Town Place at Greenbrier, businesses, hospitals, the Naval base, shopping, and restaurants galore. You'll also been within an hour of Virginia Beach and the beaches of North Carolina. At Bell Great Bridge you can enjoy urban excitement with suburban convenience. Bell Great Bridge apartments offer great features and luxury living. Our beautifully appointed homes are designed with contemporary interiors, inviting outdoor spaces and environmentally-responsible building materials. Our amenity package includes a resort-style pool, bark park, car care center, cyber cafe, and more. Call today to reserve your new home or set up a tour at Bell Great Bridge!
As you search for your perfect apartment, please keep in mind that rental rates (including specials and discounts) and availability are very fluid in the apartment industry. Based on many factors, the rental rates (including specials and discounts) and availability can actually change not just daily, but hourly, if not even more quickly. While our advertisers do take steps to keep their information current, the rental rates or availability you may find here now at ForRent.com may not be the same as when you contact the communities.
Here are some similar properties you might be interested in
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This Chicago Elementary School Makes Kids Want To Go To Class
Here at Architizer HQ, we are always thrilled when we come across a project that goes the extra mile while giving a little bit back. Well, the UNO Elementary School Soccer Academy, designed by JGMA in Chicago, does that—and much more. The project, which has won an A+ Popular Choice Award, is a model of community development and a stellar example of architecture's potential to change the world.


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Was it only a week ago this past Sunday that Secretary of State Hillary Clinton called the North Koreans "unruly children" on "Meet the Press" and said all they wanted was attention and that "we shouldn't give it to them"?
Yes, that was Hillary. The wife of the same Bill Clinton who gave Kim Jung Il 10 years' worth of free good publicity by traveling to North Korea and shining the global spotlight on the "Dear Leader's" generosity in releasing two journalists, whom Jung had illegally seized in the first place. North Korea's last good press was before 1949, but now they shine in the glow of worldwide approval thanks to Bill (and Hillary) Clinton.
Those two nuclear explosions? Hey, so what? Those rockets that can go 4,500 miles and someday hit Hawaii? Lots of countries have them. And haven't the North Koreans proven that they are just plain folks?
History is curiously repeating itself. In 1993, President Clinton was working up the gumption to impose sanctions against North Korea after they were caught enriching uranium, but his momentum -- always difficult to sustain at best -- was derailed when former President Jimmy Carter traveled to Pyongyang to announce a deal with North Korea to stop them from going nuclear. The deal turned out to be nothing more than a green light, but no sanctions were imposed.
Now former President Clinton has upended the world's efforts to isolate and punish North Korea by letting it in from the cold.
Why did he do it? He and Hillary saw a chance for positive publicity. She, newly consigned to the inside pages of the newspaper, and he, entirely absent from them, chaffed at their irrelevance and jumped at the chance to get back into the limelight.
Obama may or may not have initiated the trip, but he knew of it and approved it. Why did Obama OK it? In the upside-down world of Obama's foreign policy, the more a nation is our enemy, the more he feels he has to show it kindness, love, warmth and support. The more it is our ally (Colombia, Israel, Britain, Honduran democracy advocates), the more he must give it the cold shoulder. He calls it engagement. It is really something more than appeasement but, one hopes, less than disloyalty.
Whether Gun Violence or Abortion Violence, Harming Innocent Human Life Is Always Wrong | Ryan Bomberger
TPP Would Authorize Obama to Set $15 Minimum Wage, Card Check, and CO2 Emissions Regulation—All By Executive Fiat | Michael Hammond
The Heart of the Pro-Life Movement Is a Heart of Compassion: A Response to Colorado | Congressman Diane Black
McCain: Let's face it, Trump's rhetoric is turning Muslims all over the world against us | Allahpundit


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> Is the absence of the actual triples bothering you? If so, you're out > of luck, because there's really no good way to put them in. Drew, why should there be no good way of expressing RDF triples more directly in a rule language? Wouldn't something along the lines below, with a suitably extended semantics, work? ----------- <swrl:Variable rdf:ID="x1"/> <swrl:Variable rdf:ID="x2"/> <swrl:Variable rdf:ID="x3"/> <ruleml:Imp> <ruleml:body rdf:parseType="Collection"> <swrl:rdfTriple> <rdf:subject rdf:resource="#x1" /> <rdf:predicate rdf:resource="⪚hasParent"/> <rdf:subject rdf:resource="#x2" /> </swrl:rdfTriple> <swrl:rdfTriple> <rdf:subject rdf:resource="#x2" /> <rdf:predicate rdf:resource="⪚hasBrother"/> <rdf:subject rdf:resource="#x3" /> </swrl:rdfTriple> </ruleml:body> <ruleml:head rdf:parseType="Collection"> <swrl:rdfTriple> <rdf:subject rdf:resource="#x1" /> <rdf:predicate rdf:resource="⪚hasUncle"/> <rdf:subject rdf:resource="#x3" /> </swrl:rdfTriple> </ruleml:head> </ruleml:Imp> ---------- Gerd Wagner http://is.tm.tue.nl/staff/gwagnerReceived on Friday, 28 May 2004 05:56:36 UTC
This archive was generated by hypermail 2.3.1 : Tuesday, 6 January 2015 20:49:40 UTC


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Pet Community: Dog Community - Cat Community - All Pets - MySpace for Pets
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OFF Festival des harmonies et orchestres symphoniques du Québec
- May 05, 2016 - May 29, 2016
The OFF Festival offers a series of a hundred free musical shows at various sites in the downtown area and in severalrestaurants in Sherbrooke. Concerts with meals, outdoor shows and theme activities showcase artists from music education institutions in the area and young local talent.
Telephone : 819 821-7016E-mail
Activities and services
- Shows
- Other activities
- Bar
Restaurant and food services
- Restaurant on premises
- Picnic area
Schedules and rates
May 05, 2016 - May 29, 2016
- Free admission
- Free activities / shows


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With 1.7 million freelancers, we'll match you with the perfect talent.
The client has made the following changes to the job.
Client prefers freelancers from:
You are still able to submit a proposal for this job.
The client prefers freelancers from
a different location.
We are currently looking to develop a Reputation Management tool.
There should be 4 main features within this web application.
Once the user logs into the website they will see,
1) Scan through various forums and review sites including Facebook and Twitter
2) Identify the various posts made on a specific company (Identify through kw)
3) Identify the website
4) Quick reply button
We are trying to create a one-stop station for checking a company's review.
Please state your expected pay for such a project, time taken, and your planned outline.
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Lost Boys: The Tribe
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TVMuse Recommends
You may want to check out the following movies:
Beats Rhymes & Life: The Travels of a Tribe Called Quest (2011)
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"You can not find peace by avoiding life, Leonard." (Virginia Woolfe, The Hours)
"You can not find peace by avoiding life, Leonard." (Virginia Woolfe, The Hours)
Imagine two days full of people who get you, who get your desire for growth, who live their lives authentically, wholly and in service of the greater good.
Imagine a figurative kick in the butt so forceful that it propels you to reach higher and further towards your own best self, and that at the same time is gentle and supportive.
Imagine whole body dancing with, laughing with and hugging real amazing and interesting people who expand your vision of what is possible in this world in the best ways.
Sound delicious?
And I have yet to say a word about how wonderful, approachable and insightful the speakers were.
Alive was like meeting long lost friends I didn't know I have.
The four-story-high tree at the back of my house is blooming and buzzing with bees and other insects. The buzzing was so loud this morning, I heard it through the closed window.
That's what I call alive!
"When you leave this weekend it's going to be you vs. procrastination, vs. complacency, vs. statistics, vs. odds, vs. history."
– Greg Hartle (on why it is important to cultivate relationships and listening.)
We can't build it on our own.
"The point of engaging our fears isn't to show us that they are unfounded, but to realize that even if they are true, we are and will be ok."
'"Connectional Intelligence is the ability to build and realize value from networks of relationships, to harness units of knowledge and reuse them to innovate, to convene communities and to marshall a variety of resources for breakthrough results.
In our new world of an embedded digital infrastructure that connects all of our lives, the power of connectional intelligence holds exponential, and previously untapped, potential for breakthroughs in ways we can barely imagine."
Erica and Saj-nicole believe that contextual capacity – the ability to bring together different kinds of people and ideas to foster recombination of different ideas, and to see things from a different perspective – is a key part of connectional intelligence and a key skill for both individuals and institutions to develop if they want to remain competitive.'
From Pamela Slim's new book "Body of Work".
"So what did you do today?"
"Not much, just watch the sun rise from the ISS."
Why Americans Are the Weirdest People in the World
I stumbled on this interesting article in my feed
It describes why it is wrong for so much psychological and behavioral research to be done on American college students.
It turns out cognition is heavily influenced by culture. The implications for Dan Ariely's research then is that it is applicable only in a much narrower field than we are lead to believe.
Whenever I hear the first sentences of the Priests song in Les Miserables' prologue I start crying. There is so much love and beauty in these simple words of compassion.
"Come in, Sir, for you are weary
And the night is cold out here.
Though our lives are very humble
What we have, we have to share."
What if you had an entire week of play?
Play and it's secretly working on a project?
Play and it is secretly discovering the power of napping?
Play and it's secretly playing all the plays?
Friends of The Fluent Self already know about the power of play and the power of Rally, a group play event in Portland.
Last year I trekked (what felt like) half way around the world to take part in a Rally. This year we're bringing Rally to Europe.
We ( Hannah, Wiebke and I) have been working on this since September and it is truly my favorite project. It has been easeful, if not always easy, and processing your own stuff always came first.
If you want to know more, or join us follow this rabbit hole: http://eurorally.tumblr.com/letsplay


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What is Address Search?
Spokeo reverse address search helps people connect with their neighbors and find people by address. Search your neighborhood today to find out current residents, past residents, and family information. Find out who lives on Harris St today.
Other Spellings
Harris St is also spelled Harris Street, Harris Strt, Harris Str.
Atmore, AL
Time Zone & Current Time
Atmore, AL lies within the Central Standard Time (or CST). The current time in Central Standard Time is 08:40 AM on 11/30/2015.
Atmore, AL
Top Streets
10th Ave, 11th Ave, 12th Ave, 14th Ave, 18th Ave, 1st Ave, 1st St, 21st Ave, 2nd Ave, and 2nd St
Atmore, AL
Population Demographics
Household Income
Home Value
36 years old
53% male
Local Ethnicity
Ethnicity Ratio
About Atmore, AL
Atmore is a city in Escambia County, Alabama, United States. At the 2000 census the population was 7,676. According to the 2005 U.S. Census estimates, the city had a population of 7,530. The city was named for Mr. C.P. Atmore. The federally recognized Poarch Band of Creek Indians is headquartered here. (more on Wikipedia)


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(2015 World Red Cross Day)….It is this Friday.
The reception is being hosted by the Imperial County Chapter of the American Red Cross. The event is being held at Cheval Farms on Larsen Road in Imperial. The public is encouraged to attend and participate. The deadline for tickets is Wednesday. During the event they will recognize Red Cross volunteers and the goals of the American Red Cross. As a special presentation, local heroes will also be recognized at Friday's event. They will also announce upcoming fundraising events. The Red Cross operates on tax-deductible donations. For more information contact the American Red Cross office on Broadway in El Centro.


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MOMENTUM is a closed back, around-the-ear headphone specially designed for music lovers wanting an iconic, sophisticated appearance, luxurious wearing comfort and rich, detailed stereo sound. The headphone boasts soft breathable leather ear cushions, metal ear cups and a stainless steel ear cup slider providing maximum durability and a custom fit. Completing the package are interchangeable cables-one with an integrated smart remote with in-line microphone, and the other a standard connection cable for excellent connectivity and ease of use.
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| Email this Novel
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| Email this Novel
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These are letters I wrote for an RS project. They are from the POV of Jewish Prisoners of War during the Holocaust in WW2. The first bit is what I used as the 'description' of my piece, (the letters were tied up in a bundle along with some pictures, with the auction lot thing at the front).
This is in novel form as I didn't want to to put them all in one short story. It didn't feel right.
I know this is a touchy subject but I wanted to publish these on here to make people more aware of the horrific events. View table of contents...
Submitted:Apr 15, 2011 Reads: 74 Comments: 1 Likes: 0
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Other writing by Rhensis Dragosia-Book 1 Secrets and Fate Impossible to Accept Artemis-Why? (Virtual's Greek myth contest) Rhensis's Character contest! *closed* Smith
Romance, Historical, Fiction, Love, War, History, Adventure, Death, Drama, Action, Family, Politics, Friendship, Assassin, King, World, Revenge, Ancient, Rome, Revolution, Murder, Slavery, Mystery, Religious, Lust, Betrayal, Marriage, Princess, Kingdom


text:
BEGIN:VCALENDAR PRODID:Godengo, Inc VERSION:2.0 METHOD:PUBLISH X-WR-CALNAME:Calendar X-WR-CALDESC:This is a calendar exported from the Rivista calendaring syste m X-WR-TIMEZONE:US/Pacific BEGIN:VEVENT UID:email@example.com DTSTAMP:20151126T181139Z CONTACT:Stefano Campanini DESCRIPTION:
\n All we can think to say is "\;wow"\;\, when we co ntemplate the wines we&rsquo\;ll open for this event. \; Hands down\, only the best Merlot\, Cabernet Sauvignon and Red Blends will be served\, and you&rsquo\;ll be dreaming about these wines long after the event. RSVP is required to email@example.com or 305.455.9791.\n
\n All information is provided by the listing user and is deemed reliable bu t is not guaranteed and should be independently verified.\n\nPhone: 305.455.9791\n\n888 Biscayne Blvd.\nMiami\, FL\n33132\n\n DTSTART:20121101T190000 DTEND:20121101T210000 LOCATION:Wine by the Bay PRIORITY: SUMMARY:Miami\, FL: Wine by the Bay Presents Great Wines of California URL:http://www.winebtb.com END:VEVENT END:VCALENDAR


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Here are a few of my favorite adventure video projects. Click on the screen or title to watch. Enjoy!
Client: The Ouray Ice Park
Title: Everyone Has a First Time
Project: All Ice Park climbers remember their first experience on the man-made walls of ice.
Project: The Ouray Ice Park has "ice for everyone" regardless of age or climbing ability.
Project: Aspen Trail Finders needed a branding video to market the search engine to adventurers planning for a perfect day in the central Colorado mountains.
Client: Hotter N'Hell Hundred "Official Pace Group"
Title: Pace Group
The HHH100 Official Pace Group is the only one of it's kind - a pace group dedicated to breaking 5 hours in the countries biggest 100 mile Edurance Ride.
Client: The Ouray Ice Park
Title: Behind the Ice
The Ouray Ice Park needed a video to educate climbers about park operations and to increase memberships. The park is funded by members, donors and sponsors.
Ouray Ice Festival: Elite Climbing Competition Each year some of the best elite ice climbers in the world compete at the Ouray Ice Festival. Here is a sample of the 2014 action.
The Wind Catchers Preview Preview the short documentary film that I have been working on featuring the San Juan Airheads. I hope to finish it next winter.
Ice Frozen waterfalls are "farmed" at the Ouray Ice Park, where people risk thier lives to climb. "Ice" is winner of numerous awards from film festivals around the world.
Take Down High school wrestlers grapple to advance to the Colorado state championships the following week.
Elite Mixed Ice Competition Sampler The best ice climbers in the world compete at the 2013 Ouray Ice Festival.
Into to Ice This short sample shows the first two minutes of Chapter 1 of the "Intro to Ice" training series that I am producing for commercial release January, 2014. There are four video's in the entire series.
White Water Adventure Produced for Pagosa Outside Adventure to show the excitement of white water rafting in Colorado.
The Superdrome Experience the excitement of track racing an Olympic-class velodrome n Frisco, Texas called The Superdrome.
Final Run! The last run of the ski season shot entirely with GoPro HD cameras.
I am RBM: Darrell Green - Unicyclist. Darrell loves to ride... just not on two wheels. This video is part of the "I am RBM" marketing campaign I created for Richardson Bike Mart in Texas.
I am RBM: Derek King - Bike Commuter. Derek loves speed. And being legally blind doesn't slow him down!
I am RBM: Landon and Nicholas - Bike Brothers. Landon wanted a brother so his family traveled to an orphanage in Siberia, Russia where they found Nicholas.
I am RBM: Sebastian York - Late Bloomer. Sebastian was afflicted with tumors in his leg which kept him from off a bike until late in his childhood.
Morning Ride Ride in the peleton with Elite cyclists training for their next race.
The race for energy is always an adventure. Emerging technology like wind power is the new "Texas tea".
All video's produced, directed, photographed and edited by Barry Stevenson


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- Visual Effects
- Games
- Animation
- Interaction Design
- Indie Dev
- Blog
Wreck Center 2
Play Game Now
Disclaimer: This game is hosted on the NFLRZ and The Devil's Garage does not guarantee this link.
Wreck Center 2 is an action shooter game where Deaky D, a popular character from the NFL Rush Zone, battles endless waves of robots, collects power-ups and earns as many points as possible. Wreck Center 2 draws inspiration from the original arcade game Smash TV. Following the success of the first Wreck Center, which has over two million plays, Wreck Center 2 was designed to include more intuitive and tighter game play, tougher challenges, smarter AI and a robust engine.
Teaming up with programmer Sean James McKenzie, I worked on art and animation for the game.
Learning from the first Wreck Center's success, it was easier to implement a more fluid game mechanic for the sequel. The new challenge was to introduce entertaining elements that extended gameplay.
By introducing bonuses and power-ups that created a new set of rules, the player had a shorter learning curve to figure out what each one did. It was, ultimately, the laser tower that took the simple action shooter game to another level. The laser tower was programmed to activate at certain time intervals and shoot out powerful laser beams or a hail of cannons that would injure the player or deactivate enemies momentarily. This addition made the game more challenging because it became a vital strategy to study the laser tower's behavior and take maximum advantage of the situation.
Category: Games