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The SSD market gets passed a TRIM command
Subject: General Tech, Storage | March 7, 2012 - 01:39 PM | Jeremy Hellstrom
Tagged: western digital, ssd, hitachi, flash, EMC
Hitachi Global Storage Technologies, which was the result of the merger of Hitachi and IBM's HDD businesses, is likely being purchased by Western Digital tomorrow for about $4.3 billion. This makes sense as WD has been using Hitachi GST as a sales partner when providing EMC with high end flash disks. This deal comes on the heels of a major sell, the SSD400S flash disk which uses Intel's 34nm SLC NAND and the SSD400S-B which utilizes the new 25nm NAND developed by Intel. Check out the specifications of the flash drives as well as the new SSD company over at The Register.
"WD is buying Hitachi GST and the acquisition is expected to be formally announced tomorrow with a condition of two years of independence for Hitachi GST - imposed by a Chinese anti-competition regulator. EMC has certified Hitachi GST's SSD400S flash disks for use in its VNX mid-range unified storage arrays, including the all-flash VNX5500-F, so WD will effectively fulfil this deal once the acquisition is announced."
Here is some more Tech News from around the web:
- TSMC suddenly halts 28nm production @ SemiAccurate
- AMD cuts loose GlobalFoundries stake @ The Register
- Peter Molyneux has left Microsoft @ The Inquirer
- Workers can't escape Windows 8 Metro - Microsoft COO @ The Inquirer
- 5 Tips and Tricks for Using Yum @ The Register
- Adobe lobs out Flash update to plug 3D security hole @ The Register
- Six foot speaker shakes buildings to their foundation @ Hack a Da
- Digital Innovations Accessories @ TechwareLabs
- Cebit 2012 HardwareHeaven Coverage
Intel / Micron Flash Technology Venture Expands, Micron Assumes Two Plants
Subject: Storage | February 28, 2012 - 05:40 PM | Allyn Malventano
Tagged: micron, Intel, imft, flash, fab
Earlier today we caught some news of Intel and Micron extending their joint agreement to develop and create flash memory under the IMFT name. Along with this extension came some rearrangements to the current plan. Intel will be selling off their stake in two of the smaller fabs, located in Singapore and Manassas, VA. The sale is for $600 million, half of which will stay with Micron as a credit that Intel can use to later purchase NAND flash produced from those factories.
The 'tip of the spear' IMFT fab located in Lehi, Utah, will remain jointly owned and operated. This makes good business sense as the Lehi fab is the first to shift to smaller process nodes. IMFT announced 25nm flash memory production at this very fab in early 2010.
PC Perspective toured IMFT Utah during the 25nm launch announcement.
Some may see this as Intel taking a step away from flash memory, but I see it as quite the contrary. Micron has always tended towards being a bulk producer of memory products, while Intel are the promary innovators in the arena. This move allows Intel to focus on the bleeding edge plant while Micron handles the particulars of cranking out those technologies developed at the Lehi Plant. It is likely that the highest grade flash comes from the Lehi plant, and Intel's half of the output is more than enough to supply their SSD production lines.
Click to view full article for the press release.
Overcoming Hurdles
A paper, titled "The Bleak Future of NAND Flash Memory" was recently jointly published by the University of California and Microsoft Research. It has been picked up by many media outlets who all seem to be beating the same morbid drum, spinning tales of a seemingly apocalyptic end to the reign of flash-based storage devices. While I agree with some of what these authors have to say, I have reservations about the methods upon which the paper is based.
TLC and beyond?
The paper kicks off by declaring steep increases in latency and drops in lifetime associated with increases in bits-per-cell. While this is true, flash memory manufacturers are not making large pushes to increase bits-per-cell beyond the standard MLC (2 bits per cell) tech. Sure some have dabbled in 3-bit MLC, also called Triple Level Cell (TLC) which is a bit of a misnomer since storing three bits in a cell actually requires eight voltage level bands, not three as the name implies. Moving from SLC to MLC doubles density, but the diminishing returns increase sharply after that – MLC to TLC only increases capacity by a another 1.5x, but sees a 2-4x reduction in performance and endurance. In light of this, there is little demand for TLC flash, and where there is, it's clear by the usage cases that it is not meant for anything beyond light usage. There's nothing wrong with the paper going down this road, but the reality is that increasing bits per cell is not the envelope being pushed by the flash memory industry.
Wait a second – where is 25nm MLC?
Looking at the above we see a glaring omission – 25nm MLC flash, which has been around for close to two years now, and constitutes the majority of shipping flash memory parts currently in production. SLC was also omitted, but I can see the reason for this – it's hard to get your hands on 25nm SLC these days. Why? Because MLC technology has been improved upon to the point where 'enterprise MLC' (eMLC) is rapidly replacing SLC even despite the supposed reduction in reliability and endurance over SLC. The reasons for this are simple, and are completely sidestepped or otherwise overlooked by the paper:
- SSD controllers employ write combination and wear leveling techniques.
- Some controllers even compress data on-the-fly as to further reduce writes and provisioning.
- Controller-level Error Correction (ECC) has improved dramatically with each process shrink.
- SSD controllers can be programmed to compensate for the drift of data stored in a cell (eMLC).
Continue reading our editorial on the not-so-bleak future of NAND Flash Memory!!!
CES Storage Roundup Part 4 - Sandisk, PQI
Subject: Storage, Shows and Expos | January 16, 2012 - 06:36 PM | Allyn Malventano
Tagged: ssd, sandisk, PQI, memory, flash, CES
Sandisk had a booth with a large array of small nand flash storage devices, though most of it appeared to be SD, CF, or for embedded mobile applications:
One of the more interesting pieces was a 64GB e.MMC nand flash part that fit *within* the dimensions of a penny! This is not a plug-in module - it's the type that would be soldered onto the mainboard of a cell phone or other small mobile device:
While the booth was generally light on SSD's, there were a couple on display, namely the U100, in both 7mm (left) and 9.5mm (right) form factors:
The U100 is also available in even smaller form factor. We're currently taking a look at an Ultrabook equipped with the same Sandisk U100 SSD - mounted to an even smaller PCB.
PQI has been a favorite of mine for years. They were among the first to make a really tiny thumb drive, and I'm glad to see they continue to make a versatile line of products:
A little known fact is that PQI also has a line of SATA SSD's:
The S525 Series (also available as the S518 - 1.8" form factor), is a bit long in the tooth and uses a dated JMicron controller, but PQI made the extra effort to include the optional USB 2.0 interface that most other manufacturers chose to omit.
More to follow
I've still got some pics to sift through, so stay tuned for more CES Storage goodies!
PC Perspective's CES 2012 coverage is sponsored by MSI Computer.
Follow all of our coverage of the show at http://pcper.com/ces!
CES Storage Roundup Part 3 - Intel Cherryville and IMFT 20nm flash die spotted!
Subject: Storage, Shows and Expos | January 16, 2012 - 05:33 PM | Allyn Malventano
Tagged: ssd, micron, Intel, imft, flash, cherryville, CES, 20nm
CES is sort of like a Where's Waldo book. There are thousands of places to look, with new technology spread around all over the place. Some of that unreleased tech shows up right in front of you and you don't even realize what you were looking at until later on. It's how we caught a look at prototype Light Peak (now Thunderbolt) two years ago, and this year we saw some more goodies not previously seen in the wild. I tend to be a bit of a shutterbug, and I take seemingly random pics of things as the PCPer gang runs around the various vendor booths and hotel suites. While going through the pics from my phone, I ran across this shot of what I thought was an Intel 320 Series SSD:
Definitely not a 320, that's an Intel 520 Series (Cherryville) SSD. While Intel had their 520 Series locked up tight at their Storage Visions booth, this one was powering another motherboard makers product elsewhere in Vegas. Unfortunately this system was only to demo the motherboard itself, without a connected display, so it would not have been possible to run our own benches.
At storage visions, we also saw this display at the Micron booth. It's interesting to see how 16GB of flash memory has shrunk over the past few years. We've certainly come a long way from the good old X25-M:
Some of you may know that I'm a sucker for a good die shot, so I snuck back out to Micron's suite later on to get my own macro shot of the 20nm IMFT flash die:
Micron is, like many other vendors, working on their own SSD solution specifically for SSD caching applications. It's currently unreleased, so more to follow on this.
PC Perspective's CES 2012 coverage is sponsored by MSI Computer.
Follow all of our coverage of the show at http://pcper.com/ces!
CES Storage Roundup Part 2 - Corsair, Patriot
Subject: Storage, Shows and Expos | January 12, 2012 - 12:29 AM | Allyn Malventano
Tagged: ssd, patriot, memory, flash, corsair, CES
While roaming Vegas, we came across lots of storage goodies. Here are a few:
Corsair showed their line of SSD's, with a new addition:
The new addition is on the right. This is the 'Accelerator' series, an SSD primarily meant for caching duties:
The Accelerator series will be available in 30, 45, and 60GB capacities, and will be packaged with caching driver software for those not running a Z68 or better caching capable Intel board.
Also on display was a refresh to the Voyager, Voyager GT, and Survivor series, bringing their interface up to USB 3.0 speeds.
We also saw Patriot's lineup:
Pictured above, from top down, is the following:
- Wildfire (Sandforce 6Gb/sec / Toshiba Toggle-mode flash)
- Pyro SE (Sandforce 6Gb/sec / IMFT Sync flash)
- Pyro (Sandforce 6Gb/sec / IMFT Async flash)
- Magma (Phison / Async flash)
- Mac Series (identical Pyro SE, but Apple certified)
Next is the USB lineup, with many new USB 3.0 models replacing the older 2.0 units. The Transporter series is a bit shorter than it used to be, which is a welcome bonus.
Here are Patriot's portable flash offerings, consisting of high capacity SD cards and Phison-driven mSATA and smaller (!) form factors:
This is 'mSATA mini', which is about half the length of a standard mSATA SSD. On the other end of that spectrum is a 240GB Macbook Air unit (just off camera in the above pic).
Stay Tuned!
...I've got a few more goodies to post!
PC Perspective's CES 2012 coverage is sponsored by MSI Computer.
Follow all of our coverage of the show at http://pcper.com/ces!
CES Storage Roundup Part 1 - Toshiba, Kingston
Subject: Storage, Shows and Expos | January 11, 2012 - 09:26 PM | Allyn Malventano
Tagged: toshiba, ssd, memory, kingston, flash, CES
While roaming Vegas, we came across lots of storage goodies. Here are a few:
Toshiba was showing a 19nm flash memory wafer and all of their products containing them.
They seem to be taking their SATA SSD lines less seriously, as there were none on display. While there were no SSD's to speak of, there were USB devices:
There was also plenty of SDHC, including their own SD WiFi card - used to upload photos as they are taken.
They were also showing an SDXC card. While the shown card was a functioning 64GB unit, the SDXC format is capable of taking to cards up to 1TB in capacity.
We saw some cool stuff over at the Kingston booth:
If you look inside that case, you'll see they are now making an mSATA SSD:
They also showed their ever expanding line of USB 2.0 and 3.0 devices:
...and this really tiny model, which packs 8GB of storage into something barely big enough to unplug without the use of needle nose pliers:
Stay Tuned!
...more storage stuff is coming soon!
PC Perspective's CES 2012 coverage is sponsored by MSI Computer.
Follow all of our coverage of the show at http://pcper.com/ces!
Kingston Digital Launches its Fastest USB Flash Drive
Subject: Storage | November 17, 2011 - 12:40 PM | Jeremy Hellstrom
Tagged: usb 2.0, usb 3.0, kingston, hyperx, flash
Fountain Valley, CA -- November 17, 2011 -- Kingston Digital, Inc., the Flash memory affiliate of Kingston Technology Company, Inc., the independent world leader in memory products, today announced the Kingston DataTraveler HyperX 3.0. The DataTraveler HyperX 3.0 is designed for enthusiasts and gamers, and features the fastest speeds and largest capacities that Kingston has to offer in a USB Flash drive.
Its high-speed eight-channel architecture provides USB 3.0 data transfer rates of up to 225MB/s read and 135MB/s write. Users can save time associated with opening, editing and copying large files and applications between devices. The fast write speeds also allow users to work on large files or applications directly from the USB 3.0 drive without performance lag.
"Enthusiasts have long known HyperX as the memory of choice for overclockers and power users who need the most performance from system memory," said Andrew Ewing, Flash memory business manager, Kingston. "The new DataTraveler HyperX 3.0 USB Flash drive continues this tradition. With the fastest speeds and largest capacity, this storage device is the perfect solution for users who require high performance and carry a lot of data."
The DataTraveler HyperX 3.0 is available in 64GB, 128GB and 256GB capacities. The 256GB capacity can store approximately 10 Blu-ray Discs (25GB each), 54 DVDs (4.7GB each), 48,640 MP3s (4MB each) or 13,473,684 Microsoft Word files pages with various formatting and basic graphics.
With a durable and sleek design, the Kingston DataTraveler HyperX 3.0 is a terrific portable storage solution for gamers, enthusiasts, early adopters and high-end consumers who require the best performance and highest capacities to carry their digital library. Faster speeds and higher capacities enable users to quickly store their digital files and keep it with them at all times, in HyperX style.
DataTraveler HyperX 3.0 features a metal and rubberized casing with a reinforced key ring hole for daily use. It allows users to keep it with them at all times, as it can be easily attached to a key ring or lanyard.
A video of the device can be found here.
DataTraveler HyperX 3.0 is backed by a five-year warranty, 24/7 tech support and legendary Kingston reliability. For more information visit www.kingston.com.
Kingston DataTraveler HyperX 3.0 Features and Specifications:
- Capacities: 64GB, 128GB, 256GB
- Speed:
- USB 3.0: up to 225MB/s read and 135MB/s write
- USB 2.0: up to 30 MB/s read/write
- 8-Channel Architecture Backwards Compatible: with USB 2.0 and USB 1.1 ports
- Solid/rugged design: durable metal and rubberized casing provides increased protection
- ReadyBoost Support
- Dimensions: 2.952" x 0.916" x 0.626" (74.99mm x 23.29mm x 15.9mm)
- Operating Temperature: 32°F to 140°F (0°C to 60°C)
- Storage Temperature: -4°F to 185°F (-20°C to 85°C)
- Simple: just plug into any USB port
- Practical: durable casing with a solid lanyard loop
- Warranty: 5 Years
How about a battery free RAMdrive? The Viking ArxCis-NV writes to flash if it loses power
Subject: General Tech | October 20, 2011 - 11:35 AM | Jeremy Hellstrom
Tagged: Viking, ramdisk, DRAM to SLC, flash, super cap
Move over Fusion-io and RAMdisks with battery back up, Viking Technology has a surprise in store for you. Their DDR3 ArxCis-NV works as a standard DIMM in your machine, making installation and compatibility a snap. The difference is the super capacitor, available in a variety of sizes, which provides power long enough for the entire contents of the DIMM to be dumped to SLC flash for non-volatile storage in the case of a power outage or expected shut down. Once power is restored the contents of the SLC flash is dumped back to the DIMM and once again your storage media is back to running at DDR3 speeds. The slowest part of your storage will be the flash drive! If that sounds like something you'd like to know more about head to The Register.
"Viking Technology is a division of Sanmina-SCI, and its DDR3 ArxCis-NV is a DIMM that comes in 2, 4 and 8GB capacity points and operates at DRAM speed. It integrates into industry-standard x86 motherboards and functions in the host environment as a JEDEC standard DDR3 registered DIMM. If there is a power failure, or a host driven command, the ArxCis-NV will save all data in the DRAM to SLC (single-level cell) flash; upon power being restored, the data is written back to the DRAM ready for the system to access immediately following boot-up, provided there's sufficient operating system-level support for such a restore."
Here is some more Tech News from around the web:
- Major Chinese supplier halts rare earths production in attempt to boost prices @ Engadget
- Nvidia Tegra roadmap slips a year @ SemiAccurate
- Unbricking and upgrading an ASUS wl520 router @ Hack a Day
- Rosewill Wireless-N WiFi USB Adapter @ Benchmark Reviews
- ARM's Cortex A7: Bringing Cheaper Dual-Core & More Power Efficient High-End Devices @ AnandTech
- Can AMD survive Bulldozer's disappointing debut? @ Ars Technica
- Leaked Intel roadmap reveals PCIe flash kit @ The Register
- AMD taps Papermaster as CTO @ The Register
FeTRAM is higher and lower; gives the best of both worlds
Subject: General Tech | September 28, 2011 - 01:02 PM | Jeremy Hellstrom
Tagged: ram, FeTRAM, low power, flash
There's a new type of Flash RAM looking to make its name on the street called FeTRAM, which sounds as interesting as the phase change memory that we've been hearing about. It is an improved version of Ferrous RAM, which is very fast and uses very low power but uses a destructive reading technique. The T in the new RAM stands for transistor, so instead of the charge on the memory cell being negated by a read, the transistor will hold onto the charge so that the data can be held long term. That spells the difference between a memory module good only for RAM and a module that can be used in an SSD. The Register points to an article citing a 99% reduction in power usage when compared to current flash memory technology.
"Nanotechnology boffins are exploring a new type of nonvolatile memory that not only has the potential of being faster than today's flash RAM, but also requires 99 per cent less energy.
Called ferroelectric transistor random access memory – FeTRAM, for short – the scheme is based on a new type of transistor that combines silicon nanowires with an organic ferroelectric polymer – P(VDF-TrFE) – that switches polarity when an electric field is applied to it."
Here is some more Tech News from around the web:
- Information explosion: how rapidly expanding storage spurs innovation @ Ars Technica
- Amazon Kindle Fire Surfaces @ Slashdot
- BM partners with Intel, Samsung and TSMC for fab research @ The Inquirer
- Lenovo, Compal snuggle up to build notebook plant @ The Register
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I would like a few observations as to how Im riding. Go ahead and dish it out, I can take it. First a few fast facts so we know what we are talking about. Age 64, retired for 9 years. Most of working life was pushing a pencil behind a desk. Took up golf after retirement and saw that I was not going to get ANY exercise from that. Bought my bike about 18 months ago. Live in Florida and ride the flats, but in some pretty extreme summer heat and humidity. Rides are about 90% paved trails, and 10% roads. The bike is a very good quality road bike, very light, so I cant blame anything on it.
My standard ride, which I dont get to do nearly as often as I want due to weather, other commitments, etc. but I try hard to get out 2 or 3 times a week.
The trail I ride is 60 miles round trip, two or three intersections calling for very slow if not stopped briefly. The best I have done speed wise for the round trip is exactly 15mph, actual measured average speed.
I tend to not ride at a very steady pace. I know I start out too fast. I do a sprint or two up to 25-27 mph, but these are short lived. I also tend to chase faster riders not to catch their wheel obviously but just to see if I can keep their pace. Lots of expended energy for no really good reason?
So, should I be going lots faster? I do continue to improve slowly, and by summers end I think I can do the 60 at perhaps 17mph.
Go ahead and shoot me down. I would also like any suggestions for learning to maintain a more constant pace. Thanks.

subset: commoncrawl


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The peritoneum consists of the parietal peritoneum –
a heterogeneous, serous, semi-permeable membrane that lines the abdominal wall – and the visceral peritoneum, which covers the abdominal organs (Figure 1). Its surface area is approximately 1-2 m2. In males, the peritoneum is a closed-sac system, whereas in females it is an open-sac system with the fallopian tubes and ovaries connecting to the peritoneal cavity. The parietal peritoneum derives its blood supply from the abdominal wall (lumbar, intercostals, and epigastric regions) and drains into the inferior vena cava, while the visceral peritoneum receives its blood supply from the superior mesenteric artery and drains into the portal vein. The total peritoneal blood flow ranges from 50–150 mL/min. The peritoneal cavity, located between the parietal and visceral peritoneum2, contains approximately 100 mL of serous fluid1 and becomes the dialysate compartment during peritoneal dialysis (PD) from which exchange of solutes with the blood can occur1,2. Drainage of the peritoneal cavity is mainly accomplished by the lymphatic system. Importantly, the subdiaphragmatic lymphatic system is responsible for 70-80% of the lymphatic flow from the peritoneal cavity. The lymphatic system also serves as a pathway for the removal of foreign substances and macromolecules. In stable patients undergoing PD, the rate of lymphatic flow varies from 7-20 mL/hr with total fluid losses between 60-91 mL/hr3.
Six Layers of the Peritoneum
The peritoneal membrane is comprised of six layers consisting of the capillary fluid film, capillary endothelium, endothelial basement membrane, interstitium, mesothelium, and the fluid film (Figure 2). It was previously thought that all six layers provided different levels of resistance to solutes, and the mesothelial layer with its large surface area functioned as the dialyzer. However, the three-pore model (below) suggests that the peritoneal capillary is the critical barrier to peritoneal transport.
The Three-Pore Model
The three-pore model (TPM) of the peritoneum defines solute and water transport across the peritoneal capillary through pores of three different sizes: Large, small, and ultra-small pores4. This model has been validated by clinical observations5,6. The large pores range from 10-20 nm (100-250 A°) in size and are formed by clefts between endothelial cells. Large pores are small in numbers, < 0.01% of total pores, and account for < 10% of solute removal (macromolecules). Small pores are more numerous, also formed by clefts between endothelial cells, account for > 90% of solute removal (small solutes) and water, and range from 4-6 nm in diameter. Ultra-small pores, comprised mainly of Aquaporin-1, range in size from 0.4-0.6 nm and are transcellular channels in the endothelial cells that transport water only and provide 0% of solute removal. The transport of water via the Aquaporin-1 is also known as "free-water transport" and contributes to ~50% of the ultrafiltration in PD4,7.
Although this model suggests that the interstitium may also contribute to resistance to solute transport, there is no resistance from the mesothelium itself or from stagnant fluid layers. Additional research has expanded on this model. Ronco proposed that the peritoneal vasculature, particularly the surface area of the peritoneal capillaries, rather than the entire surface area of the peritoneum and the interstitium, is responsible for facilitating solute transport8. Specifically, the distance of each peritoneal capillary from the mesothelium determines that capillary's relative contribution to effective surface area and the resistance properties of the membrane. This concept is called the "nearest capillary hypothesis", where the capillaries closer to the mesothelium experience a greater osmotic effect compared to those farther away. Since patients with the same total peritoneal surface area may have very different degrees of peritoneal vascularity, their effective peritoneal surface areas would also vary widely. Moreover, in a single patient, peritoneal surface area may be altered by specific events such as episodes of peritonitis9.
P/N 102479-01 Rev. A 12/2014


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Sponsored Financial Reporting
Sponsored Financial Reporting (SFR) is both a set of business processes and the institutional unit reporting to the Controller's Office that is responsible for submitting financial reports and invoices to sponsors to fulfill award requirements. Most of the steps in the sponsored financial reporting business process are completed in cooperation with (or by) SFR staff accountants. For example, SFR staff prepare sponsor-required financial invoices and reports with assistance from Principal Investigators and Department Administrators. Invoices and reports are prepared based on the frequency and due date requirements established by the sponsor and written in the award documents.
Sponsored Financial Reporting receives all revenue on behalf of the University for sponsored grants and contracts. SFR collection representatives follow up with the sponsor on all past due invoices to ensure receipt of payment on invoices and work with both sponsors and academic departments on quality or quantity issues related to non-payment.
Sponsored Financial Reporting is responsible for closing all sponsored awards in the financial system once the award is over, all spending complete and all payments received.
Sponsored Financial Reporting is responsible for coordinating sponsor, state and federal financial audits including the A-133 audit of expenditures of federal awards. They provide information for the University's annual reports, supplemental schedules and consolidated financial statements.
Uniform Guidance 2014
As you know, we will be facing significant policy, procedure, business process, system, and training changes in the months ahead as we implement the new federal Uniform Guidance (replacing OMB Circulars A-21, A-110, and A-133), as well as implement other significant agency changes, such as the move to NIH subaccounts.
We are most grateful to the U community for the great response to our request for volunteers to help with the Uniform Guidance. We have created seven Uniform Guidance Work Groups, shown below, in addition to our Uniform Guidance Steering Committee and some very talented individual specialists.
- Costing
- Pre-Award / Sub-Award
- COI Work
- Purchasing
- Property
- HR / Effort
- Post-Award
You can follow the work of these groups, as well as the continuing national efforts and agency implementation guidance, on our new Uniform Guidance web site, at: http://www.ospa.umn.edu/documents/UG.html.
How to
How to prepare your sponsored award for close out
Closing a sponsored award in Peoplesoft Financials is a joint effort between SFR and the academic departments. SFR has created a checklist (pdf) as a tool to facilitate the close out process. Below are some resources that support the process on the checklist.
How to process journal entries to fund cost share on sponsored projects and do an F&A redistribution
Training Services has created a document titled Analysis Types (pdf) that has useful information and sample journal entries for the two analysis types introduced in March 2010: Cost share revenue (CSR) and Reallocate F&A (RFA). More information about Analysis Types can be found here (pdf) in "slide" format.
How to move payroll and fringe on sponsored projects
An announcement was made December 13, 2011 about changes to this process. Read the brief announcement of the change on the EFS message screen archive here and more details about the procedure itself here.
NIH Salary Cap
A job aid and queries are available to assist department staff with NIH salary cap payroll and cost sharing calculations. Posted on July 17, 2009.
Did you know that there is a fair amount of confusion when departments calculate the cost sharing towards payroll expenses for individuals who exceed the NIH Salary Cap? To help alleviate this issue SFR and Training Services created a job aid to assist users with calculating payroll and cost-sharing associated with the NIH Salary Cap. The job aid provides a high-level summary of the NIH Salary Cap process and procedures as well as links to a helpful spreadsheet and queries payroll and accounting staff can utilize in calculating and monitoring cost-sharing for individuals exceeding the NIH Salary Cap.
The job aid (pdf) is located on the Training Services website.
Salary Cap Summary (FY1990 - FY2010)
Payroll and cost share calculation worksheet (xlsx)
Special website for sponsored activity
SPA and SFR jointly created an EFS Sponsored Projects Web site that provides comprehensive information of all the changes related to sponsored projects due to the implementation of the new financial system in July 2008. Follow this link to visit the site.
Frequently asked questions
Departments typically have 60 days or fewer after the end date of the sponsored project to record activity in the general ledger, depending on the sponsor and type of award--please read the information below. Contact your Grant Administrator in SPA or your Grant Accountant in SFR for more specific deadlines for your project.
If for some reason there is activity that belongs on a sponsored account but the deadlines have passed, contact SFR as soon as possible. The staff there can provide further guidance.
For Federal awards the University generally has 90 days to liquidate all encumbrances and submit the final financial report. In order to meet that deadline all transactions should be processed within 60 days of the end date so that the final financial report can be prepared and still allow the PI and department adequate time to review it before it is submitted to the sponsor. If departments were allowed the full 90 days to process transactions, there would not be adequate time to prepare, review, and submit the report before the sponsors deadline.
The deadlines are different if the University is a subrecipient on a Federal award. In these cases the University has 60 days to submit a final report/invoice to the institution that we received the subaward from so that they can incorporate that information into their final financial report to the prime sponsor by the 90 day deadline. For these types of awards departments would have less than 60 days after the end date to process transactions.
PeopleSoft Financials will stop any transactions beginning at 90 days after the end date.
For non salary journal entries, the only difference when processing the journal entry is the CF1 value should be YR000020XX (the XX represents the FY of the original transaction).
The Historical Salary Adjustment (HSA) processing window for sponsored or cost share ChartField strings is now a rolling five years. It remains the case that HSAs on sponsored accounts are expected to be processed within the period allowed under policy (currently, within the active Effort Certification period). This change does not impact this requirement. Instead, this change is meant to allow the system to technically allow HSAs outside of this window (in those rare cases when these are approved) as opposed to being forced to use a journal entry for corrections older than 26 pay periods.
After reviewing several options and working with the module support team we have a long term solution to the situation. Change the GPC processing box to begin at 7:00 pm - this would ensure the box is complete by the time the copy of PRD to RPT is made and remove the dependency that the other modules processing boxes complete before the GPC box starts.
How does this change impact the academic departments? Transactions processed on a project on a Monday in the feeder modules are cost collected into GPC on Tuesday's GPC nightly processing box run which starts at 7:00 pm and will be in the reporting instance Wednesday morning. This gives consistent and accurate data and departments can feel confident that "everything" is there when running sponsored reports out of PS Reporting Instance or UM Reports.


text:
OSI-Approved Open Source (21)
- GNU General Public License version 2.0 (19)
- GNU Library or Lesser General Public License version 2.0 (7)
- Apache License V2.0 (5)
- BSD License (5)
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- Artistic License (2)
- Academic Free License (1)
- Attribution Assurance License (1)
- Open Software License 3.0 (1)
- PHP License (1)
- wxWindows Library Licence (1)
- Other License (2)
- Windows (79)
- Linux (61)
- Mac (58)
- Android (42)
- Grouping and Descriptive Categories (33)
- Modern (12)
- Other Operating Systems (6)
- Embedded Operating Systems (4)
Top Apps
- Audio & Video
- Business & Enterprise
- Communications
- Development
- Home & Education
- Games
- Graphics
- Science & Engineering
- Security & Utilities
- System Administration
yunus and my other projects
Yunus is a simple "visual" script language. Yunus is obsolete (left in 2004). However this site exhibits many projects; you will find my other "PHP, VS.NET, Flash, Delphi" projects. eOgr is my second complete application after Yunus.93 weekly downloads
Libre Photo Illustrator
Illustrator for free/libre photos that are in the public domain or at least published under one of the CC-BY licenses. This sidebar widget has the aim to enrich your weblog with selectableand configurable OpenContent materials.
R3 Registration System
R3S is a simple registration system for events of any kind and size. It is written in Perl, using the Catalyst MVC framework and uses jQuery.. It has two levels of access, featuring mail confirmation, reports and GPG-signed certificates.
OpenRecord is a web-based collaboration system. It's designed to be similar to a wiki, but with some additional database features.
Mundo PlaneShift
Spanish translation of PlaneShift, CrystalSpace and CEL documentation and localization of these apps. and libs. Traducción al español de la documentación de PlaneShift, CrystalSpace y CEL, así como la localización de esas aplicaciones y librerías.
Perl Beginners Site
A Portal site for Perl Beginners that aims to be a useful first stop in their quest to learn more about Perl.
Eyecandy/Braincandy Quake Engine derived from TomazQuake.
Fallen Warrior
You are a Knight, hack , slash , stab and build your way through the game. Use arrow keys to move click on people to talk or double click to kill.
busca dentro de archivo vcf y crea tantos archivos como contactos hay1 weekly downloads
Adobe AIR app (Windows OSX Linux) for renaming movie files & DVD folders, downloading posters, and creating .url shortcut links to IMDB. Uses NFO file when available. Remembers words to save (720p, DTS) and remove (like release group names).17 weekly downloads
Quiz Web App done for a UML/Software Design course.
MPages Commons: Open MPage Development
Collaborative project between academic medical centers to develop rich internet applications. The products of this collaboration will be shared with other institutions.1 weekly downloads
C Preprocessor for ActionScript
Integration of Make/C Preprocessor into Adobe Flex 2 SDK, along with IDE integration tips and some handy preprocessor macros and classes. Works under Windows or Linux, with GNU tools or Microsoft tools.1 weekly downloads
RED3GL examples code tutorial
bidi for flash
trying to properly display combined hebrew and english text in flash but you can't because flash is lame? here lies an implementation of the bidirectional algorithm in actionscript.
Simple UGC site with Flex and PHP
This is a simple UGC site sample made with Flex and PHP. For more information, please visit project web site (http://ugcwithflexphp.sourceforge.net/)
qxbbs toolbox
QXtoolbox is a free open source end user tool to better use the internet more safely and more convenient.
GUI tool for Flex applicaions layout composition. Provides basic functionality of the Flex Builder Design View.3 weekly downloads
Profile IT is a FLASH CMS that generate FLASH CONTENT at run time and at the same time also generate a HTML page that is mobile friendly of the very same site (WYSIWYG for both of version)
Min hemsidan som jag försöker fixa med foton4 weekly downloads
2D 3D Flash Fighting Game
Open Source Game is a 2D 3D fighting game based in flash. Please help us to devlop our game!2 weekly downloads
Better THings To Do
A Small website/screensaver to remind us to leave our desks and have some fun, and let your cheesy screensaver play
The Immortals Website
This project is the development of a Content Management System (CMS) based website for a Lord Of The Rings Online kinship group, located on the Brandywine server, named "The Immortals".
This is an mmorpg for u 2 enjoy. it is very good and you will like it, it has different classes which makes it different from other mmorpgs.I am going to add a lot of updates to it. Many people should play this game for my and your benefit.
VideoChatFlex is a webchat build with flex and flash media server, and will have the limit of 20 users with camera and mic online. and you can see who's online, who have camera and mic, and also a text based chat.


text:
With 1.7 million freelancers, we'll match you with the perfect talent.
The client has made the following changes to the job.
Job awarded.
Client prefers freelancers from:
You are still able to submit a proposal for this job.
The client prefers freelancers from
a different location.
This assignment is for an ebook that provides tips on networking which includes social media tips. The ebook would be approximately 15 - 18 pages (8 1/2" x 11") double spaced. A sample tip booklet is attached
The title of the tip book is The New Networking: 111 Ways to Become a Magnetic Networker . The writer would provide all content. The writing must be original (no plagiarism) and I would own all copyrights to the material.
Need completed document within 5-6 days upon acceptance of bid.
Sign in or Register to see more


text:
Book your room at Quality Inn
Standard Room, 2 Queen Beds, Non SmokingMax Occupancy: 4 guests (up to 3 children, 3 infants)
Standard Room, 1 King Bed, Non SmokingMax Occupancy: 2 guests (up to 1 child, 1 infant)
Get an overview of this hotelHotel in Mankato with free breakfast and indoor pool
Situated in Mankato, this hotel is within 6 mi (10 km) of Verizon Wireless Center, Blakeslee Stadium, and Minnesota State University. Mount Kato Ski Area is 6.6 mi (10.6 km) away.
Hotel Features
An indoor pool, a spa tub, and dry cleaning are available at this hotel. Free buffet breakfast, free WiFi in public areas, and free self parking are also provided. Additionally, a 24-hour front desk, gift shops/newsstands, and a front-desk safe are onsite.
Room Amenities
Rooms feature free WiFi, coffee makers, and cable TV. Guests will also find satellite TV, free local calls, and hair dryers.Information missing or incorrect? Tell us!
Hotel Amenities
Hotel Amenities
Quality Inn features an indoor pool and a spa tub. High-speed wireless Internet access is complimentary. Guests can enjoy a complimentary breakfast. This Mankato hotel also offers gift shops/newsstands, dry cleaning/laundry services, and a safe deposit box at the front desk. Onsite self parking is complimentary.
- 24-hour front desk
- Dry cleaning/laundry service
- Free breakfast
- Free self parking
- Free WiFi
- Gift shops or newsstand
- Indoor pool
- Safe-deposit box at front desk
- Spa tub
Available in all rooms: Free WiFi
Available in some public areas: Free WiFi
Free self parking
Room Amenities
- Air conditioning
- Cable TV service
- Coffee/tea maker
- Free local calls
- Free WiFi
- Hair dryer
- Iron/ironing board
- Pay movies
- Phone
- Satellite TV service
Where to Eat
Guests are offered a complimentary buffet breakfast.
Nearby Things to Do
An indoor pool and a spa tub are on site.
To make requests for specific accessibility needs, complete the "Special or Accessibility Requests" section of "Trip Preferences" when you book your hotel.
- In-room accessibility
Hotel Policies
Check-in time starts at 3 PM
Check-out time is 11 AM
Payment types
Children and extra beds
- Children are welcome.
You need to know
Extra-person charges may apply and vary depending on hotel policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
Optional extras
- Pet fee: USD 15.00 per night


text:
Quick Links
Contact Information:
Department of Toxic Substances Control
2014/15 Green Ribbon Science Panel Members
The 2014/15 Green Ribbon Science Panel has 15 members consisting of reappointed members from the first Panel and new experts. The Panel meets approximately twice a year; additional meetings are scheduled as needed. Meetings are subject to the Bagley-Keene Open Meeting Act.
The service term of the prior membership is completed under the provisions of the statute.
The Green Ribbon Science Panel will continue to advise the Department on a variety of scientific and technical matters related to Green Chemistry. Some specific topical subjects will be related to aspects of the implementation of the Safer Consumer Product regulations. As the Safer Consumer Product program moves forward and matures, the Panel may also tackle other topics. Refer to the Past Meeting Archives and Subcommittees pages to see topics discussed at past meetings.


text:
Holly Madison Twitterview
- Photo: RD/Kabik/Retna Digital1 of 11
By Dana Flax
Curious what exactly former "Girl Next Door" star and Playboy model Holly Madison has to do with Pee-wee Herman? Check out the transcript from our Twitterview with the reality star to learn about her E! show, "Holly's World," her favorite Vegas flicks and what she looks for in a guy! (Oh, and then make sure you're following @WonderwallMSN and @hollymadison123 on Twitter.)
@WonderwallMSN: Hey @hollymadison123! You ready to start this Twitterview?
@hollymadison123: I'm ready to start my interview!
Get out the vote
Show CommentsNextWhen using Facebook Connect your image and name may display on Wonderwall. All Privacy Settings are controlled by Facebook
- button.LOL
- button.AWW
- button.OMG
- button.FAIL


text: My team is sponsoring a 20 mile TT an I will be designing the T-shirt. Does anyone have a good image of a racer getting held at the start of a TT in an elevated gate. I would prefer a picture from the front looking straight at the racer. I plan just to use the silhouette and trace the entire scene. I won't put actual faces. Either PM me or attach a thumbnail. Thanks everyone!


text:
A $400 marble angel on a headstone at Whiteford Union Cemetery was reported stolen Friday, the Monroe County Sheriff's Office said.
A woman called authorities after she visited her son's grave and discovered the statue missing from the top of her son's headstone. She last saw the statue Oct. 6 and reported the theft to the grounds keeper.


text:
Nanoscribe sets new benchmarks for microfabrication.
Our integral approach combines a disruptive 3D printing technology with user-friendly software and innovative materials leading to reproducible and lean processes.
Our customers are innovators. They overcome prevailing technological boarders and serve a broad spectrum of applications in science and in emerging markets.
Tiny 3D-printed gift for Chinese President
November 19, 2015
During his visit of the Hamlyn Centre at Imperial College London, the Chinese president Xi Jinping was very…
GT-User Meeting Europe 2015
October 13, 2015
We are proud to announce that our GT-User Meeting Europe has been finished very successfully! We thank all …
MRS Fall
Boston, MA (US), December 01 - 03, 2015
Booth: 830
BiOS Expo
San Francisco, CA (US),
February 13 - 14, 2016
Booth: 8740
Photonics West
San Francisco, CA (US),
February 16 - 18, 2016
Booth: 431 (Hall A)


text:
Aviation Place
402 E. Aviation Blvd., Universal City, TX
Are you the manager of this property?Click here to claim this listing Check Your Credit Now
About Aviation Place
Come visit Pebble Beach Village. A pleasant, affordable place to call home! A hop, skip & a jump to Randolph Air Force Base. Conveniently located to Retama Park and local shopping malls. Easy access to most major highways and near the Schertz, Cibolo, Universal City Independent School District. Come home to our 1 & 2 bedroom apartment homes with Gas and Electricity Paid! Enjoy our built in bookcases/dressers, mini-blinds, spacious closets and extra storage available. Our community has 2 on-site clothes care center, 2 Sparkling Swimming Pools and best of all Large Pets are Welcome! Come home to Pebble Beach Village Today!
As you search for your perfect apartment, please keep in mind that rental rates (including specials and discounts) and availability are very fluid in the apartment industry. Based on many factors, the rental rates (including specials and discounts) and availability can actually change not just daily, but hourly, if not even more quickly. While our advertisers do take steps to keep their information current, the rental rates or availability you may find here now at ForRent.com may not be the same as when you contact the communities.
Here are some similar properties you might be interested in
Gold Talon
Universal City, TX
From $515
Studio - 2 Beds
Sable Ridge
Universal City, TX
From $505
Studio - 3 Beds
Sycamore Creek
Schertz, TX
From $585
Studio - 2 Beds
Waterford Park
Converse, TX
From $760
1 - 3 Beds


text:
By ROBERT PETŘÍK on Jun 16, 2009 | Reply Lucky Day Lottery NL
Promotional/Sweepstakes Office
Laan van Hoornwijck 55
2289 DG Rijswijk
KvK: 41151075
The Netherlands.
Dear. Robert Petřík
We are pleased to inform you that your email address has won 1,520,000:00 Euro (One Million Five Hundred & Twenty Thousand Euro Only) in the Netherlands Lucky Day Lottery Sweepstakes promotional program, conducted on the 2nd of June 2009, and the results were release today 16th of June 2009. This is a free promotional program sponsored by consortium of software companies, which mean Lottery tickets were not sold.
For more information's/procedure of your winning claim, you are advice to contact our processing department with the contact information below, provide them with your winning details below and your winning email address.
Bob Bongers (Mr).
Lottery Processing Dept.
Tel: +31 647231839
Fax: +31 847512664
Fax: +31 847512664
Email: email@example.com
Your Winning Details.
Ref No: STLI/772-5/7009
Batch No: 889/5557JQ.
E-Ticket No: ZUK-7708-217-777
Serial No: NL/9667/04465
NOTE: Please be warned, your winning and its entire information are to be kept strictly confidential, this is to avoid previous bad experience this program has suffered, such as abuse of this program by other internet user who use the name of this company for unscrupulous activities and double claiming of winning entitlement because of insecurity of winning information on the part of beneficiaries. Always call to ensure you are dealing with the right office.
Mrs. Maltida Brook.
Sweepstakes Coordinator.
Visit our website at: http://www.luckyday.nl
Lotto is een onderdeel van De Lotto. Copyright(c) 2007 by De Lotto, the Netherlands.
The Free Lotto Awards is organized by consortium firm companies to encourage the use of the internet and promote computer literacy worldwide.
Any views of this e-mail are those of the sender except where the sender specifically states them to be that of Lucky Day Lottery or its subsidiaries. The message and its attachments are for designated recipient(s) only and may contain privileged, proprietary and private information. If you have received it in error, kindly delete it and notify the sender immediately. Lucky Day Lottery accepts no liability for any loss or damage resulting directly and indirectly from the transmission of this e-mail message.


text:
"Your reputation certainly precedes you and, based upon my first hand
observation, it is a reputation that is well deserved."
For 25 years, Gary McGowan has served as an arbitrator and mediator in both national and international disputes, often complex, high-stakes matters.
An attorney for 40 years, McGowan practiced business litigation for 16 years, most notably as a founding partner of Susman, Godfrey & McGowan, a highly successful boutique litigation firm. As a neutral, he has established a reputation for effectiveness, thorough preparation, and quick discernment of the pivotal points for deciding or resolving a dispute.
Based on a national survey, Law Dragon 500 named him as one of the 500 best judges (both public and private) in the United States, and the Texas Lawyer, relying on a survey of Texas lawyers, picked him as one of five "Top Notch" arbitrators and mediators in Texas. Texas Monthly has selected him as a Texas Super Lawyer in each of the last nine years.
Recent Publications
12 Ways to Achieve Efficiency and Speed in Arbitration, published in Corporate Counsel. http://www.law.com/corporatecounsel/PubArticleCC.jsp?id=1202596761854&12_Ways_to_Achieve_Efficiency_and_Speed_in_Arbitration
Sanctions in US and International Arbitration: Old Law in Modern Context, published in Kluwer Arbitration Blog. http://kluwerarbitrationblog.com/blog/2013/10/10/sanctions-in-us-and-international-arbitrations-old-law-in-modern-context/
Don't Call It A Trial: What Eavery Lawyer should Know About Arbitration, published in Houston Lawyer, July-August 2014. http://issuu.com/leosur/docs/thl_julaug14/15?e=0
Recent Honors:
Accepted to the Energy Arbitrators List, a global panel of energy industry arbitrators sponsored by the International Centre for Dispute Resolution. http://energyarbitratorslist.icdr.org/ealsearch/faces/eal?_afrLoop=5143603581967443&_afrWindowMode=0&_adf.ctrl-state=14s53gax6c_4
Listed on Institute for Energy Law Directory of Energy Arbitrators.
Fellow, Chartered Institute of Arbitrators (London).
Member, Commercial Panel, Large Case Panel, and Oil & Gas Panel, American Arbitration Association.
Member, International Panel of Arbitrators, International Centre for Dispute
Resolution (New York), a division of the American Arbitration Association.
Member, Roster of Arbitrators, American Health Lawyers Association.
Selected by Texas Lawyer as one of five "Top Notch" neutrals in Texas.
Listed in 21st Edition of The Best Lawyers in America in the practice area of Arbitration.
Listed in The International Who's Who of Commercial Mediation 2013 (WHO'S WHO LEGAL, London).
Panel of Arbitrators, Hong Kong International Arbitration Centre, http://www.hkiac.org/en/arbitration/arbitrators
Member, Board of Directors, American College of Civil Trial Mediators.


text:
Washoe Sheriff Haley Announces He Won't Run for Third Term
Updated: 01/17/2014 - Washoe County Sheriff Mike Haley announced Thursday he will not run for a third term.
Updated: 01/17/2014 - Washoe County Sheriff Mike Haley announced Thursday he will not run for a third term.
Updated: 01/16/2014 - Carson City's fire chief says the powder that led to an evacuation of the state Supreme Court building is not hazardous.
Updated: 01/16/2014 - A Nevada-based contractor will be remodeling John Ascuaga's Nugget Casino Resort in February for a $50 million property-wide renovation program.
Updated: 01/16/2014 - Just like yesterday, highs in the 50s.
Posted: 01/16/2014 - Back in early January, Lyon County Sheriff's deputies began an investigation of a possible homicide taking place at a house located at the 100 block of Railroad Street in Dayton. Officials now say this allegation is unfounded.
Updated: 01/16/2014 - Nobody is seriously hurt in a crash in Lemmon Valley Thursday morning, according to Reno police.
Updated: 01/16/2014 - The U.S. Department of Agriculture has designated nine counties in Nevada as primary natural disaster areas due to the drought.
Posted: 01/16/2014 - Reno police are investigating a stabbing Wednesday night.
Updated: 01/16/2014 - There may not be enough water to support recreation this summer in the Truckee River.
Updated: 01/16/2014 - While Tahoe Resorts are getting the short end of the stick this season, golf courses are benefiting. With the dry climate, snow lovers are trading in their ski poles and snowboards for golf clubs.
Updated: 12/17/2014 - An iconic business which helped introduce generations of northern Nevada to music is closing its doors.
Updated: 01/16/2014 - Want to send your child off to a weekend of fun?
Updated: 01/16/2014 - The Rosewood Lakes golf course isn't self-sustaining, and is costing Reno half a million dollars a year to maintain, according to a consultant.
Updated: 05/12/2014 - Champagne Family Dentistry is providing underprivileged children with free dental services during its 'Give Kids a Smile' event.
Updated: 01/15/2014 - For the 23rd year, KTMB's Christmas Tree Recycling enabled residents to recycle their family holiday trees for use in area park and fire protection projects, instead of sending them to the landfill.
Posted: 01/15/2014 - Are you in the market for adopting a horse?
Updated: 01/15/2014 - Expect another sunny afternoon with above average highs.
Updated: 01/15/2014 - Reno police have arrested a Reno man accused of stabbing a man in the downtown area.
Updated: 12/17/2014 - Reno's Maytan Music Center is closing soon, after being open since 1959.
Posted: 01/15/2014 - A Reno gang leader heard on jailhouse telephone recordings describing himself as a "shot caller" has been sentenced to up to life in prison after he was convicted of ordering the shooting death of a 16-year-old rival gang member.
Posted: 01/15/2014 - With the roll-out of the new Driver Authorization Cards which allow undocumented people to drive legally on Nevada's roads, many people speculated the cost of auto insurance could start to decline. But insurance experts say it is going to take some time before we can really determine how the cards will affect rates.
Updated: 01/14/2014 - There's no lack of opinions about the Sparks City Council's approval of a WalMart at Legends, but they may have had no options.
Updated: 01/14/2014 - Firefighters are investigating a house fire on Meadowlark Circle in the Gardnerville Ranchos in Douglas County Tuesday night. Flames broke out around 8:45 p.m.
Updated: 01/14/2014 - With a very mild winter thus far, businesses in Tahoe City are hoping for more snow to help their bottom line.
Posted: 01/14/2014 - Reno fire crews are investigating an apartment fire Tuesday night. Flames broke out at the Rosewood Park Apartments on Mira Loma Drive around 6:00 p.m.


text:
HeadFirst EJB 3.0 Book wrote:The @Table.UniqueConstraint attribute allows you to specify unique column constraint.
JPA Specs- (9.1.4) wrote:The UniqueConstraint annotation is used to specify that a unique constraint is to be included in the generated DDL for a primary or secondary table.
Christophe Verré wrote:It allows to generate unique constraints in the DDL. Unique constraints are explained here. (I don't think this kind of annotation is at the exam, but who knows...)


text: Bearing in mind the difference in labeling that Zvonimir mentions, if you are used to G2 with other papers, you may prefer the middle grade of Emaks (G3 from Freestyle). I tend to use more G2 than G3 and very little G4, but others use G3 as their normal grade. G2 is a little softer than other G2 papers, and G3 is a little harder. I think when J&C was selling it they called the middle grade "Normal," though Cachet and Maco labeled it G3, and Freestyle labels it G3.


text:
PLEASURE UNDER THE SUN (3) by Lindsay Evans: Determined to become a partner at her firm, financial advisor Bailey Hughes spends far too much time working and not enough time having fun. Artist Seven Carmichael makes it his mission to uncover Bailey's fun side and force her to see that there's more to life than making and saving money. But first, he must penetrate Bailey's aversion to artists, brought on by the unstable, hippie lifestyle she grew up with. There was a slight disconnect between the two main characters, but the storyline is entertaining. The author has exceptional skills in painting the scenery, making it easy for readers to visualize the story as it unfolds.
Reviewed by:
B. Nakia Garner


text:
Djafar S.
My name is Djafar, and I have 3 years of work experience as a senior care worker. I found this job interesting. I have met lots of nice people and I can call them my friend(s) now. I can provide all home support services such as cooking, transportation, grocery shopping, and daily check up. I am flexible with timing and days. Currently I am living in San Jose and I can drive 40 miles away. For more details please contact me.


text:
Oklahoma Businesses Making Strides with Employee Health Incentives, but Challenges Remain
Davis, KirLee, THE JOURNAL RECORD
For a microcosm of what's behind Washington's health care debates, one need look no further than the challenges businesses face adapting employee wellness practices.
Widely seen as one step to help contain a firm's growing health care costs, Oklahoma's Certified Healthy Business Program was started in 2003 to recognize firms providing employee health and wellness incentives and services. More than 900 firms have received certification statewide, Tulsa Health Department Wellness Coordinator Jill Almond said, up from 497 in 2008. She said 66 Tulsa businesses have received certification, with 16 establishing tobacco- free policies.
"If your business is healthy, if people recognize that, it shows what your focus is," Almond told a Tuesday morning audience at the Tulsa Metro Chamber's Health Care Forum. "It shows what you believe in."
A number of companies have taken steps supporting healthier lifestyles, from providing exercise breaks and fitness center memberships to encouraging nutritional snacks and meals. However, Almond said many businesses fall short of enacting actual written policies.
"A lot of elemental pieces are in place," she said. "But policy is where we really need to hang our hat. We really need to develop that."
That stumbling block may reflect a number of factors, from embarrassing employees and forcing them to confront personal weaknesses to angering those seeking to protect personal freedoms.
"It's such a personal habit that we didn't want to step on anyone's toes," Plan Benefits Analysts Nutrition and Wellness Specialist Beth Miller said of adopting a tobacco-free policy.
Tulsa's Inverness Village embraced the healthy business concept.
"We really wanted a wellness culture," Wellness Director Jana Headrick said.
However, the retirement community operator has struggled with putting some sort of wellness performance measurements into a policy form.
"We're still kind of fumbling with that," Headrick said.
Cultural habits tend to interfere at Explorer Pipeline and others.
"We like to eat and we like to celebrate and we like to have that family culture," said Explorer Compensation and Benefits Administrator Michelle L. Griffith. "Those are all wonderful things."
But since that translates into frequent morning doughnuts and numerous birthday cakes, she said it naturally offsets many corporate wellness efforts.
"That is an area we struggle with and trying to find that balance where we can celebrate in other ways," she said. …


text:
heather grey long sleeve 'Rosangela Pina' linen chest pocket tee
designer's notes
Linen provides a special drape fit that's exceptionally chic. This long sleeve tee from Vince embodies easy, elegant style you can wear all year.
color: Heather Lightweight linen knit Crewneck Long sleeves Rounded hem 100% Linen; Hand Wash; Imported; style# 328528001


text:
We sell specialty waxes from usa
Witcovar 146 Sonneborn
Packing 23.5 kgs
Contact our sales
Microcrystalline Wax Applications:
Hot Melt Adhesives
Ink Grades
Laminating Grades
We Are KIRANA TIGA BINTANG INDONESIA Company
We are chemicals company of specialty applications
We sell additive for plastic industrial manufacturer
We are polymer compounders and masterbatch....
We sell Titanium Dioxide KRONOS
Form powder white
Packaging 25 kilograms paperbag
For plastics which require high outdoor durability and very good optical properties.
Uses in PVC, ....
Phone: + 62-87821111445. + 62-8567288845
Kitachem Ekasedjati Company
Purwokerto, Indonesia
SUPPLIER of Special Chemicals = TRUST and FRIENDLY SERVICES = Fine chemicals... WE ARE SUPPLIER OF....
Heat Resistant Paint Coating or Fire Retardant Paint Coating for protective and protection material from high temp products manufacturer industry
Heat resistant paint and heat resistant coating....
Paint coating industry that is the best paint coatings product company industry International
Industrial paint coating manufacturers, paint coating companies, protective and protection paint coating....
Arsih Natural Paint , give the texture of the exterior Natural Stone Looks, combines elements of natural, luxury natural stone, and contribution to the the environment preservation.
We use natural....
We produce Bamboo Furniture, Bamboo House and Gazebo, Bamboo Home and garden accesories, Lamps, carvings, and Rustic Furniture, in Export Quality.
We create various range of product from Gazebo & ....
F probes measure the thickness of material non - magnetic ( eg paint , plastic , porcelain enamel , copper , zinc , aluminum , chrome etc. ) on magnetic
materials ( eg iron , nickel etc. ) . often....
General Supplier distributor& representative of laboratory, medical and industrial Equipment
Gum Rosin ( Gondorukem) Local quality of the results of Pine Gum Processing Factory Industrial Workers in Indonesia.
Products are packaged according to customer demand / buyers.
Available for....
CV. Idea Cipta Sentosa founded on 2007 in district of Pekalongan province Central Java Indonesia as entity in general trade activity and consultancy business.
We have business networking in Central....
Thinner products for furniture painting and car body painting.
We are looking for automotive paint products to distribute in Central Java and Jogjakarta


text:
Spring training breakdown: Twins 5, Pirates 4
Pirates/MLB Videos
Tuesday's game
Orioles at Pirates, 1:05 p.m.
McKechnie Field, Bradenton, Fla.
Webcast: pirates.com
Pirates starter: LHP Wandy Rodriguez
Orioles starter: RHP Chris Tillman
Monday's result
Twins 5, Pirates 4
Hammond Stadium, Fort Myers, Fla.
Twins starter: Vance Worley 2 IP, 2 H, 1 SO
Pirates starter: Kyle McPherson 2 IP, 1 H
Boom: Jose Tabata got his first hit, a solo homer to left that tied the game at 4 in the seventh inning. Tabata did not play in the Grapefruit League opener, and he has come off the bench in the past two games.
The decider: Left-hander Kris Johnson gave up the tiebreaking run in the eighth. Chris Colabello's two-out double scored Brandon Boggs from first base.
Stock up: Jordy Mercer batted in the 2 spot and had two hits, both of which moved a baserunner from first to third and led to runs.
Stock down: RHP Jeanmar Gomez was torched for four runs in 1 1⁄3 innings. He gave up three hits (two of them doubles) and walked two.
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PLM implementation for manufacturing
PLM implementation for manufacturing News
April 28, 2015
Manufacturing pioneers embrace product as a service
Despite the hoopla over the IoT, it's still early days for product as a service as manufacturers steer a slow course to new business models.
March 21, 2012
Medical device maker puts product compliance tools at everyone's disposal
SAP PLM integration gave Varian compliant designs, but downstream enforcement at sales, supplier and production stages proved even more critical.
November 23, 2011
Study finds PLM software moving into process manufacturing
Research firm ARC Advisory Group also says more manufacturers are using PLM-based social media along with improved PLM-MES integration and three-dimensional simulation.
May 13, 2011
Harris integrates Agile PLM with Oracle Autovue to boost document visualization
To boost its document visualization capabilities, the communications equipment maker integrated Oracle AutoVue with Agile PLM. But seamless integration remains a work in progress.
PLM implementation for manufacturing Get Started
Bring yourself up to speed with our introductory content
Which technologies do companies need to support product as a service?
While companies may be considering the product as a service trend, most will need to make some profound changes to their technology infrastructure -- and their perspective on product lifecycle management -- to make the new model work. Continue Reading
How can manufacturers improve field service operations?
Equipment providers and distributors are shifting their focus to improving field service. What are some best practices and technologies that can boost customer engagement and increase sales? Continue Reading
product lifecycle (in marketing)
The product lifecycle, in a marketing context, is all the stages of a product's life span that are related to its promotion and sales. The marketing lifecycle is traditionally broken down into four stages: introduction, growth, maturity and decline. Continue Reading
Evaluate PLM implementation for manufacturing Vendors & Products
Weigh the pros and cons of technologies, products and projects you are considering.
What can PLM technology do for enterprises?
Product lifecycle management can be an opaque topic. Here's help understanding exactly what PLM technology is and how it can help your company innovate. Continue Reading
Digital manufacturing helps manufacturers reduce risk
Digital manufacturing software helps manufacturers plan, simulate and optimize production processes before adding costly infrastructure and tooling. Continue Reading
Manufacturers eye product as a service as new cash cow
For some, making a thing and selling it is so 2000-whatever. Instead, those companies are creating ongoing revenue streams on after-sales services. Continue Reading
Product ReviewsPowered by IT Central Station
It makes searching for parts easy with the Pro/E data, but the publishing and viewing process needs to be simplified.Powered by IT Central Station
Valuable Features: The connection between the search engine and parameters in the Pro/E data makes searching for parts easy. •...Continue Reading
Manage PLM implementation for manufacturing
Learn to apply best practices and optimize your operations.
How SAP QIM provides comprehensive insight into customer complaints
Companies can use SAP's Quality Issue Management application to get a 360-degree view of customer complaints, from their origin through the complaints handling process. Continue Reading
Product data management software: Secret weapon for manufacturing performance?
Manufacturers today rely on technology such as product data management software to manage critical product data, especially when problems arise. Continue Reading
Manufacturers consider the product as a service trend
Manufacturers are starting to embrace the product as a service trend -- but correctly pricing the service component could be a steep challenge. Continue Reading
Problem Solve PLM implementation for manufacturing Issues
We've gathered up expert advice and tips from professionals like you so that the answers you need are always available.
Manufacturing PLM tools beginner's tutorial
In this guide, learn the basics of PLM tools, including software selection, implementation, and cloud computing options. Continue Reading
SCM PLM integration gives manufacturers greater supply chain perspective
Manufacturers are seeing the connection between supply chain management (SCM) and product lifecycle management (PLM) software. Through SCM PLM integration, problems in the supply chain can be address -- and solved -- with greater ease. Continue Reading
PLM-supply chain integration helps manufacturing firms see the big picture
To get the most out of product lifecycle management (PLM), manufacturers need to integrate it with all parts of the supply chain, including supply chain management (SCM). Continue Reading


text:
Automating the world one-liner at a time…
A couple a weeks ago I got a call from BillG's Technical Assistant (TA) telling me that Bill and Ray (Ozzie) wanted to get a demo of the new stuff we were doing with PowerShell. The setting was Bill's conference room that would be organized with a number of Demo stations. You'd get called in and were allowed to bring one other person but then it would be Bill and Ray sitting next to you getting the demo – so you pretty much have their full attention. That's great except for the fact that I am a bad typist and make mistakes all the time. That is why PowerShell supports wildcards, parameter disambiguation and tab completion – you benefit from my flaws. J Still, this was all the new stuff and had never been demo (to anyone) before and wasn't even to ready for alpha testing so I was taking a risk in showing it.
In the past, I dealt with this sort of situation by having a text file which contained all the things I wanted to type in it. Doing a cut-n-paste from notepad during a demo looks REALLY bad so what I would do is to paste the entire sequence into a PowerShell session. This would execute all the commands and then I would use the CMD.EXE command history feature and use the UP arrow to get back to the first command and then just type ENTER and DOWNARROW to rerun the demo one command at a time. This allows you to focus on WHAT you want to say instead of putting all your mental energy into typing things correctly. This is what I did when I did a demo at Bob Muglia's keynote address in Barcelona last year.
Let me be clear on this point - this technique doesn't work very well! It's a toss up as to whether it is better or worse they just typing things in from scratch. The big problem in both Barcelona and in the BillG/RayO demo was STATE. When you run a command, it can change the state of the system so you might not be able to just go to the top of a demo and run it all over again because the state might be changed. The other issue is that the CMD.exe mechanism is pretty fragile. If you change the command in any way shape or form, it decides that it is a new command and thus it resets your position in the command history to be at the end. ARRRGGG! I'll spare you the horror stories and get to the punch line. I decided I had had enough of that nonsense and decided to write a script which would run a demo for me. I've called this function: Start-Demo. I've attached it to this blog entry. I have to say that I'm pretty please with how it came out.
I strongly encourage you to use this if you ever have to give a demo – it totally rocks! It completely transforms the experience of giving a demo, allowing you to focus on your messages instead of typing. It is REAL in the sense that the commands REALLY run, the script just eliminates your typing. I put in all sort's of "grace notes" including:
I think you'll find some pretty useful techniques in the script itself so even if you do use it for your demos, you might want to review the script:
function Start-Demo{ param($file=".\demo.txt", [int]$command=0) Clear-Host $_lines = Get-Content $file $_starttime = [DateTime]::now Write-Host -for Yellow "<Demo [$file] Started>" # We use a FOR and an INDEX ($_i) instead of a FOREACH because # it is possible to start at a different location and/or jump # around in the order. for ($_i = $Command; $_i -lt $_lines.count; $_i++) { $_SimulatedLine = $("`n[$_i]PS> " + $($_Lines[$_i])) Write-Host -NoNewLine $_SimulatedLine # Put the current command in the Window Title along with the demo duration $_Duration = [DateTime]::Now - $_StartTime $Host.UI.RawUI.WindowTitle = "[{0}m, {1}s] {2}" -f [int]$_Duration.TotalMinutes, [int]$_Duration.Seconds, $($_Lines[$_i]) if ($_lines[$_i].StartsWith("#")) { continue } $_input=[System.Console]::ReadLine() switch ($_input) { "?" { Write-Host -ForeGroundColor Yellow "Running demo: $file`n(q) Quit (!) Suspend (#x) Goto Command #x (fx) Find cmds using X`n(t) Timecheck (s) Skip (d) Dump demo" $_i -= 1 } "q" { Write-Host -ForeGroundColor Yellow "<Quit demo>" return } "s" { Write-Host -ForeGroundColor Yellow "<Skipping Cmd>" } "d" { for ($_ni = 0; $_ni -lt $_lines.Count; $_ni++) { if ($_i -eq $_ni) { Write-Host -ForeGroundColor Red ("*" * 80) } Write-Host -ForeGroundColor Yellow ("[{0,2}] {1}" -f $_ni, $_lines[$_ni]) } $_i -= 1 } "t" { $_Duration = [DateTime]::Now - $_StartTime Write-Host -ForeGroundColor Yellow $("Demo has run {0} Minutes and {1} Seconds" -f [int]$_Duration.TotalMinutes, [int]$_Duration.Seconds) $_i -= 1 } {$_.StartsWith("f")} { for ($_ni = 0; $_ni -lt $_lines.Count; $_ni++) { if ($_lines[$_ni] -match $_.SubString(1)) { Write-Host -ForeGroundColor Yellow ("[{0,2}] {1}" -f $_ni, $_lines[$_ni]) } } $_i -= 1 } {$_.StartsWith("!")} { if ($_.Length -eq 1) { Write-Host -ForeGroundColor Yellow "<Suspended demo - type 'Exit' to resume>" $host.EnterNestedPrompt() }else { trap [System.Exception] {Write-Error $_;continue;} Invoke-Expression $($_.SubString(1) + "| out-host") } $_i -= 1 } {$_.StartsWith("#")} { $_i = [int]($_.SubString(1)) - 1 continue } default { trap [System.Exception] {Write-Error $_;continue;} Invoke-Expression $($_lines[$_i] + "| out-host") $_Duration = [DateTime]::Now - $_StartTime $Host.UI.RawUI.WindowTitle = "[{0}m, {1}s] {2}" -f [int]$_Duration.TotalMinutes, [int]$_Duration.Seconds, $($_Lines[$_i]) [System.Console]::ReadLine() } } } $_Duration = [DateTime]::Now - $_StartTime Write-Host -ForeGroundColor Yellow $("<Demo Complete {0} Minutes and {1} Seconds>" -f [int]$_Duration.TotalMinutes, [int]$_Duration.Seconds) Write-Host -ForeGroundColor Yellow $([DateTime]::now)}
Let's see it in action. NOTE - I've gone back and colored my INPUT red so that you can see it more clearly. It will just be normal when you run it.
PS> Start-Demo<Demo [.\demo.txt] Started>[0]PS> # ****** PowerShell is a SHELL *****[1]PS> hostname?Running demo: .\demo.txt(q) Quit (!) Suspend (#x) Goto Command #x (fx) Find cmds using X(t) Timecheck (s) Skip (d) Dump demo[1]PS> hostnamejpsvista1[2]PS> net useNew connections will be remembered.Status Local Remote Network-------------------------------------------------------------------------------Unavailable Y: \\axp-test\builds\vistartmMicrosoft Windows NetworkUnavailable Z: \\powershell\public Microsoft Windows NetworkThe command completed successfully.[3]PS> notepad#2[2]PS> net useS<Skipping Cmd>[3]PS> notepadd[ 0] # ****** PowerShell is a SHELL *****[ 1] hostname[ 2] net use********************************************************************************[ 3] notepad[ 4] # ******* Structured Commands ******[ 5] Get-Process -ProcessName lsass[ 6] gps -p lsass[ 7] gps l*s[ 8] gps |Where {$_.Handles -ge 500} |Sort Handles |ft Handles,Name,Desc* -Auto[ 9] gps [b-t]*[c-r] |stop-process -whatif[3]PS> notepad#6[6]PS> gps -p lsassTDemo has run 1 Minutes and 52 Seconds[6]PS> gps -p lsassHandles NPM(K) PM(K) WS(K) VM(M) CPU(s) Id ProcessName------- ------ ----- ----- ----- ------ -- -----------1377 21 8872 5168 71 111.97 592 lsass[7]PS> gps l*s!GPS *SHandles NPM(K) PM(K) WS(K) VM(M) CPU(s) Id ProcessName------- ------ ----- ----- ----- ------ -- -----------755 6 2276 3844 91 13.38 456 csrss762 13 7076 23340 211 291.06 516 csrss1369 20 8872 5168 71 111.97 592 lsass778 4 3020 2920 40 0.05 508 psxss299 8 5416 6744 43 20.14 568 services32 1 248 536 4 0.11 388 smss[7]PS> gps l*s!<Suspended demo - type 'Exit' to resume>PS> gps c*sHandles NPM(K) PM(K) WS(K) VM(M) CPU(s) Id ProcessName------- ------ ----- ----- ----- ------ -- -----------751 6 2276 3844 91 13.38 456 csrss760 13 7076 23340 211 291.19 516 csrssPS> exit[7]PS> gps l*sHandles NPM(K) PM(K) WS(K) VM(M) CPU(s) Id ProcessName------- ------ ----- ----- ----- ------ -- -----------1369 20 8872 5168 71 111.97 592 lsass[8]PS> gps |Where {$_.Handles -ge 500} |Sort Handles |ft Handles,Name,Desc* -AutoQ<Quit demo>
Jeffrey Snover [MSFT]Windows PowerShell/MMC ArchitectVisit the Windows PowerShell Team blog at: http://blogs.msdn.com/PowerShellVisit the Windows PowerShell ScriptCenter at: http://www.microsoft.com/technet/scriptcenter/hubs/msh.mspx
A keeper! This is terrific stuff, please keep it coming.
Very nice, thanks!
This will be handy for those of us that are doing demos to try to convince the "higher ups" that we need to build our administrative apps on Powershell (ala Exchange). :)
Very nice but you didn't tell us what Bill and Ray thought about the demo. :-)
> Very nice but you didn't tell us what Bill and Ray thought about the demo. :-)
My badge still works. :-)
I have done demos using the cut and paste method and it does look clumsy but it is better than trying to type - especially long lines.
This is a superb idea and I'll be trying it out later in the month
This is going to make life much easier for demos - thanks for sharing
freakin amazing stuff Jeffrey!!
i hope the next version of vista just boots straight into powershell, with no other gui at all.
During my webcast on 5/3/2007 I used jeffrey's start-demo script to run my powershell demos. Lot of folks
Great script. But it doesn't seem to work for my variables that I am using in my demo...
Try this in your demo.txt:
$f = dir *.*
I changed this line, and things seem to work better for me.
Invoke-Expression $($_lines[$_i] + "| out-host")
Invoke-Expression $("(" + $_lines[$_i] + ")" + "| out-host")
Thanks for the great site,
- Joe
Tried to run but no result come out...(No error and just a return) please help
So embarrassed to ask, but start-demo doesn't run in POwershell for me and I am sure there is a simple thing to do to make it happen. I tried including it in the v1.0 directory and it will run, but just leaves me with a normal powershell prompt. Do we put it in a profile or put it in a directory. How do I use this amazing tool?
-Gordon - Toronto
Jeffry Snover and many other have used Start-Demo in powershell.exe http://blogs.msdn.com/powershell/archive/2007/03/03/start-demo-help-doing-demos-using-powershell.aspx
Jeffry Snover and many other have used Start-Demo in powershell.exe http://blogs.msdn.com/powershell


text: You can get baby room monitors that are one way transmitters. You hear them but they don't hear you. They are available at most Wal-Mart/Target kinds of stores and for sure they could be found online. Under $20 too, what a deal! Just pick the place for the transmitter that will be the most advantageous for you to listen to while you print.


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Thursday, July 30, 2015
Awarded By
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Award Amount
$1,000 annually, non-renewable.
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Deadline Info
Applications and information about this award are available on the Grapevine Evaluations website at the address provided. In addition to the completed application form, students must submit the follo ... sign up for full scholarship deadline information.


text:
A native of St. Croix in the U.S. Virgin Islands, Raja Bell intends to spend part of his offseason vacationing with family in the Caribbean. He plans to play plenty of golf over the next few months, too, which shouldn't prove problematic when home is in Miami.
But perhaps more than anything, the Jazz shooting guard -- now that the 2010-11 NBA season is in his rearview mirror -- looks forward to taking some time improving his aim.
"I'll be shooting a lot of jump shots this summer," the veteran guard said.
"Make no mistake, this year I felt it (his shot) failed me a little bit," Bell added. "But I didn't do enough work last summer (in 2010), so I'll do my best ... to try to work that up."
Bell's offseason routine last year was thrown off in part by a wrist injury that required surgery in 2009 and cost him an overwhelming majority of the 2009-10 season, which he started with Charlotte and finished with Golden State.
The Jazz signed the 11-year pro anyway last July, using a three-year, $10 million contract offer to keep him from joining the Los Angeles Lakers and instead bring him back for a second stint in Utah.
But the lack of summer shooting cost both Bell and the Jazz, evidenced by disappointing 40.9 percent shooting from the field, his worst in the last seven seasons. Bell's 35.2 percent shooting on 3-point attempts also was the first time in seven consecutive seasons that he's been below 40 percent from behind the long-distance line.
"I think he felt like he had an up-and-down year," Jazz general manager Kevin O'Connor said of Bell, who averaged 8.0 points -- his lowest scoring average since the 2002-03 season -- while starting 63 of 68 games played for Utah. "Defensively, he felt like he did a great job. But he said, 'My bread-and-butter was my jump shot,' and he said, 'I wasn't as good at it as I had been.' I'm just repeating what he said."
If there was a saving grace for Bell, it was that he was mostly healthy throughout the season. He missed a few games with adductor muscle issues and a few more with a calf strain. He also missed the season's final seven games with a sprained right foot but admitted afterward that once the Jazz were eliminated from playoff contention he chose not to play simply "because it wasn't worth the risk." The wrist, though, held up just fine.
"I felt really good. I'm not upset about this season physically," the 34-year-old said. "My body was really, really good."
A 27-13 start for the Jazz was highlighted without a doubt by what has been dubbed the "Miracle in Miami," a Nov. 9 overtime win over the Heat in which Paul Millsap beat the buzzer with a putback to force OT and finished with a career-high 46 points. The Jazz rallied from 22 down, including 19 at halftime. Millsap made a career-high 19 field goals, including three uncharacteristic three-pointers in the final 30 seconds of the fourth quarter. Not to be forgotten either is Al Jefferson's last-second tip of a Devin Harris miss for a two-point win at Toronto on March 9.
It would be easy to point to coach Jerry Sloan's Feb. 10 resignation as the turning point in the Jazz season, and just as easy to look at the trade of All-Star point guard Deron Williams to New Jersey about two weeks after that. But reality is that after opening the season 27-13, Utah went 12-30 the rest of the way -- and the slide started well before Sloan and Williams exited. A six-game losing streak in January hurt tremendously, as did the fact that the Jazz went 4-10 in Sloan's final 14 games -- including that aforementioned six-game skid.
Copyright (C) 2011 The Sports Xchange. All Rights Reserved.


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Patrick Carrier spreads herbicide for crabgrass control April 13 at a home in Montezuma. (Photo by Michael Reilly / DN-R)
Broadway local Patrick Carrier began Blue Ridge Turf, LLC. (Photo by Michael Reilly / DN-R)
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Hi all,
I've been working with the GS115 strain of P. pastoris for the past month. I'm using Invitrogen's EasySelect kit, and I did not have any issues with the cloning or selection (all clones are zeocin-resistant and show desired inserts). In the beginning I was working with the X33 strain and had no issues with growth or expression, then my cells suddenly wouldn't grow well in MGY media, only YPD. Even when I inoculated MGY with a strong YPD culture of the yeast the growth was weak. I switched strains to GS115, proceeding from scratch, and I see the same issue. It's been 4 days since I picked colonies from zeocin plates into MGYH media, and the cultures are very weak and smell sour. I've tried varying glycerol, YNB, and biotin sources to make sure I wasn't using a bad reagent, and I keep the shaker set at 220rpm, 28 degrees. Is it an issue with pH or some other factor?
Any advice? I need strong MGY/MGYH cultures to get our fermenter going. Thanks!
Submit your paper to J Biol Methods today!
Pichia growth in MGY/MGYH media
Started by sd_labber, Jul 10 2012 09:23 AMPichia Minimal Media Yeast Protein Expression
1 reply to this topic
Posted 10 July 2012 - 09:23 AM
Posted 19 July 2012 - 10:22 AM
Update: for whatever reason, we figured out that our Pichia strains (X33 and GS115) would not grow in generic YNB, only Invitrogen's YNB. Very frustrating! Unfortunately, the cultures are still not very strong, but I plan to try a few other conditions to improve their strength.
Also tagged with one or more of these keywords: Pichia, Minimal Media, Yeast, Protein Expression
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text:
To reauthorize the DC opportunity scholarship program, and for other purposes.
Be it enacted by the Senate and House of Representatives of the United States of America in Congress assembled,
This Act may be cited as the ``Scholarships for Opportunity and Results Act'' or the ``SOAR Act''.
Congress finds the following: (1) Parents are best equipped to make decisions for their children, including the educational setting that will best serve the interests and educational needs of their child. (2) For many parents in the District of Columbia, public school choice provided under the Elementary and Secondary Education Act of 1965, as well as under other public school choice programs, is inadequate. More educational options are needed to ensure all families in the District of Columbia have access to a quality education. In particular, funds are needed to provide low-income parents with enhanced public opportunities and private educational environments, regardless of whether such environments are secular or nonsecular. (3) While the per student cost for students in the public schools of the District of Columbia is one of the highest in the United States, test scores for such students continue to be among the lowest in the Nation. The National Assessment of Educational Progress (NAEP), an annual report released by the National Center for Education Statistics, reported in its 2009 study that students in the District of Columbia were being outperformed by every State in the Nation. On the 2009 NAEP, 56 percent...