text:
Case Study: Describes issues of healthcare data breaches, and offers solutions from Intel Security Group that can protect healthcare organizations.
Case Study: Explores issues associated with healthcare data breaches and details solutions offered by Intel Security Group, which has a broad portfolio of software and hardware solutions to lower risks and protect healthcare organizations.
White Paper: Describes how Intel IT's SaaS security best practices enhance security and allow business groups to quickly adopt new SaaS solutions.
White Paper: Discover Intel IT's SaaS security best practices, which recommends a well-defined strategy and reference architecture, a way to balance risk and productivity, SaaS security controls, and vigilant review of technology developments.
In this video, Intel CISO Malcolm Harkins gives his unique point of view on security and social computing, suggesting we embrace it to reduce risk.
Intel CISO Malcolm Harkins gives his unique point of view on security and social computing. In this video blog, Malcolm suggests we should embrace social media and social computing to reduce risk.Full View >
Video blog: Intel CISO Malcolm Harkins shares why Intel IT has undertaken a radical new five-year redesign of our information security architecture.
In this video blog, Intel CISO Malcolm Harkins talks about why Intel IT has undertaken a radical new five-year redesign of our information security architecture. Malcolm says that compromise is inevitable under almost any compute model, find out why.Full View >
Intel CISO Malcolm Harkins talks enterprise security and BYOD consumerization and the challenges of balancing security and employee expectations.
Intel CISO Malcolm Harkins discusses the benefits, challenges, and opportunities for enterprise security and BYOD consumerization, including how to maintain security while keeping the policy flexible enough to meet employee expectations.Full View >
User Guide: Describes the Intel® Active Management Technology Configuration Utility, including prerequisites, and security and network requirements.
User Guide: Describes how to use the Intel® Active Management Technology Configuration Utility (Intel® AMT Configuration Utility), which configures systems to use Intel® AMT, including user prerequisites, and security and network requirements.
White Paper: Intel® AES New Instructions set, guidelines and demonstrations.
This white paper gives an overview of the Advanced Encryption Standard (AES) algorithm and the Intel® AES New Instructions. It provides guidelines and demonstrations to write secure, high performance AES implementations.
Intel® Advanced Encryption Standard Instructions update
White Paper: guide reviews steps to configure a server and client to use Intel® AES New Instructions (AES-NI) when performing secure web transactions.
This guide will review the steps to configure a server and client to use Intel® Advanced Encryption Standard New Instructions (Intel® AES-NI) when performing secure web transactions.
Guide: a short test case on how to use Intel® AES-NI with Oracle Transparent Data Encryption* (TDE).
This guide outlines a short test case on how to use Intel® AES-NI with Oracle* Transparent Data Encryption (TDE). Intel AES New Instructions (AES-NI) was first introduced in March 2010 and are also on the Intel® Xeon® processor codenamed Westmere-EX.


text:
With world-renowned brands like EIZO, Alwan, Shuttle, EPSON, Microtek, X-Rite and more
To cater to our diverse clientele across the middle east, we house experts of various industries and offer distinctive solutions.
We offer state-of-the-art solutions that are built to deliver precision and efficiency in your business-related graphical activities.
As the authorized regional service center for various high-end products, we observe quality standards and service diligently.
IDN's latest stock of recommended products
We operate under a quality management system which complies with ISO 9001:2008.
We are part of a group that's been in the business of advising, consulting and supporting clients of the graphics industry for over 30 years.
For all your business IT needs, no matter which industry you belong to.
Specialized workforce that can guide your business setups to adopt smart solutions for an efficient and effective change.

subset: commoncrawl


text:
< Back to front page Text size – +
Patriots trade No. 28 pick to New Orleans Saints
Posted by Zuri Berry, Boston.com Staff April 28, 2011 11:07 PM
FOXBOROUGH -- The Patriots traded their No. 28 pick in the NFL draft to the New Orleans Saints for two later picks.
The Patriots give the Saints their No. 28 in the first round for the Saints' second round pick (No. 56 overall) and a first round pick in 2012.
The Saints selected Alabama running back Mark Ingram with the pick. With the trade, that should be the end of the Patriots' day in the draft.
Now, the Patriots have five picks tomorrow in the second and third rounds. The Nos. 33 (from Carolina), 56 (from New Orleans), 60, 74 (from Minnesota) and 92.
News, analysis and commentary from Boston.com's staff writers and contributors, including Zuri Berry and Erik Frenz.
Tweets from @GlobePatriots/boston-com-patriots
get updates
browse this blogby category


text:
America's SBDC Iowa helped Erika Jensen with business plan development, market research, financial projections and more so she could expand her online chocolate business into a chocolate shop and café located in downtown Algona.
America's SBDC Iowa helped Aaron Serrano develop a business plan, identify target markets, and make the right connections so he could grow his military rewards program.
Alex Dinh and Emily Embree received help from America's SBDC Iowa in writing a business plan and negotiating a lease so they could open their own professional nail salon. Read More...
America's SBDC Iowa assisted Sandra Hendred with business plan development, financial projections, Quickbooks instruction, and more so she could successfully open her frozen yogurt shop. Read More...
America's SBDC Iowa helped Ann March with business plan development, financials and general business advice so she could start her dog daycare business. Read More...
America's SBDC Iowa helped Jim Shepherd with financial projections, market research, business planning, and more so he could start his racing car engine development company. Read More...
America's SBDC Iowa provided business plan assistance, market research, financial projections, and more to help Joe Roush open his vehicle repair and restoration business.
Read More...
America's SBDC Iowa assisted Paul Kongshaug and James Hoeger with business plan development, financial planning, and other necessary advice and connections so they could start a multi-faceted marketing and loyalty platform business. Read More...
Pete and Tara Wright received market research and business plan development from America's SBDC Iowa and were then able to open their own plumbing, well, and septic service business.
Read More...
Leonard Hoogerwerf and Kirk Brown received business advice and assistance from America's SBDC Iowa so they could further develop and market their environmentally-friendly products. Read More...
We provide FREE ONLINE BUSINESS TRAINING. Click here to try it out!
Ofrecemos la formación empresarial en línea gratis. Haga clic aquí para probarlo!
TO FIND YOUR NEAREST BUSINESS ASSISTANCE CENTER, Click Here.
For Frequently Asked Questions (FAQs), Click Here.
America's SBDC Iowa provides free, confidential, customized, professional business advice and consulting in all 99 Iowa counties to entrepreneurs and existing businesses with 500 employees or less. If you're looking for help writing a business plan, planning a marketing strategy, developing cash flow projections, doing market research, or much, much more, you've found the right organization. Along with individual business consulting, our regional business assistance centers also present affordable workshops that teach practical skills and techniques, conduct research, provide comprehensive information services, and offer access to subject matter experts in a variety of fields. Our organization is the only nationally-accredited entrepreneurial development program in Iowa.
Our state center, located at Iowa State University in Ames, is open Monday through Friday from 7:30 a.m. to 4:30 p.m. central time. Our 15 regional business centers are open similar hours, depending on their host institutions' policies. To contact the business center nearest you to find out its open hours, go to our Services page.
To find out how we can help you and your business, call the SBDC State Center at 515-294-2030, or visit our Services page to find the business assistance center nearest you.
For special business tips that might be helpful, follow us on Facebook and Twitter.
Funded in part through a
cooperative agreement with the
U.S. Small Business Administration.
An outreach program of the Iowa State University College of Business.
© 2014 Iowa Small Business Development Centers
| Credits: Development
| Login


text:
- Age / Gender:
- 21, Male
- Location:
- cheese world
- Joined:
- 7/7/07
- All Stats >
I like Newgrounds, a lot, and I like videogames as well. I have 6 cats and a dog. My best friend in real life and on Newgrounds is Dekuboy (Evan). Thats about it. Also, i like to go outdoors and i like nature and animals.
User Statshot
- Community Stats
Level 11 Blank Slate
Normal Whistle
Ranked as Civilian
Contact Info / Websites
- robotcheesehead
Latest News
Does anyone want a custom background for their desktop or laptop? If so, I will make one. I will have a sample in a few. In your response, if you want one, fill out an order.
1. Main theme
2. Colors
3. Details
4. Any words?
5. Username
Here is an example:
Favorite Audio
|Video Game Song
|Your Prelude (True)
|Cinematic Song
Favorite Movies
to the bomm sheltard! Rated 3.1837 Stars The bomm sheltar is once again invaded, requiring the gang to go and liberate it. Comedy - Parody to the bomm sheltar! Rated 3.7918 Stars A Spy Cheez It box infiltrates the Clock Crew Headquarters. Sweet, sweet love ensues. Comedy - Parody Charlie the Unicorn Rated 4.4299 Stars Charlie the Unicorn gets a surprise visit! Comedy - Original The Ultimate Showdown Rated 4.3087 Stars Everyone is fighting for ultimate superiority. Yes, even batman. Comedy - Parody
Favorite Games
UO:Defender Map Editor Rated 3.4311 Stars Map Editor for the game UO:Defender ! Fishy ! Rated 4.1138 Stars ! Fishy ! Skill - Collect Bubbles 2 Rated 4.1861 Stars The sequel to Bubbles, with new power-ups and a physics based 2 Player modus! Skill - Avoid Gravitee Rated 4.2209 Stars Gravitee is basically golf in space! Sports - Golf


text:
Campsites in England
Browse our selection of the best
campsites in England,
or click to refine your campsite search
Loveland Farm
The Snug and Hut at Park Farm
York Cycle Stop
Salhouse Broad
Ten Acres Vineyard
Tregarton Park
Vallis Veg
Baby Moon Glamping
Cobbleacre Holidays
Britchcombe Farm
Wimbles Farm
Fen End Farm
East Thorne Glamping
The Secret Campsite
East Sussex's best kept secret...
Eco Camp UK at Beech Estate Woodland Campsite
Wild glamping and tent pitch camping in a wonderful Sussex woodland hideaway.
Bloomfield Camping
True organic, family glamping in the heart of the Dorset countryside.
Eco Camp UK at Wild Boar Wood
Eco-friendly wild glamping in a magical woodland in West Sussex.
The Sunny Field
A convivial rural campsite with 15 pitches in a hilltop grassy meadow in the Garden of England. Play in the wildflowers, cook atop your campfire, or just plain chill.
Housedean Farm Campsite
An isolated walker's paradise hidden sweetly on the South Downs Way.
Northmoor Lock Paddocks
Back-to-basics camping and unbeatable canoeing on the gorgeous Oxfordshire stretch of the Thames Path.
Farrs Meadow
A charming Dorset campsite overlooking the beautiful Stour valley and a short walk from both the river and the market town of Wimborne Minster.
Thistledown Farm
Get back to nature with this agricultural treat...
Town Farm
If you're looking for a quick weekend break from the big smoke, then this is the campsite for you...
Roll on the river and run through the dreamy meadows at this oh so enchanting site...
Westland Farm
How often do you get to stay in a hand-built home?
Hook Farm Caravan and Camping Park
Find the inner-archeologist at this Dorset treat – Indiana eat your heart out...
Hole Station Adult Only Campsite
Opening its gates to campers as recently as 2009, Hole Station has already become a firm favourite among the camping fraternity, which is hardly surprising given its secluded woodland pitches, campfire encouragement, and natural beauty.
Gooseberry Field Campsite
Luxury glamping accommodation in an old orchard cast against the pleasant greenery of rural Kent.
Hooks House Farm
Hooks House Farm provides an easy going, friendly and attentive site and service. You'll be hooked. Heh.
Nethergong Camping
A marsh frog, heron and a erm...wardobe. Here you'll find the balance between peaceful campsite and the kids' Narnia playground almost perfect.
Greenacres Camping
When it comes to encouraging wholesome outdoor play for the kiddywinks, this site has it sorted with a capital S.
Granary Barn Camping
A warm welcome is guaranteed at this refreshingly basic tents-only site between Helston and Porthleven.
The Hideaway at Baxby Manor
Chilled out camping and glamping in the heart of North Yorkshire.
Treen Farm Campsite
Beach babes and sun lovers, oil up and grab your flip-flops – Treen Farm campsite is just a towel's throw from some of England's finest beaches.
Cotswolds Camping, Charlbury
Civilised and simple campsite surrounded by all the delicious Oxfordshire and Cotswolds village food offerings.
South Penquite
Come to the Fairmans' organic farm on the fringes of Bodmin Moor for a taste of luxury in one of their Mongolian glamping yurts or to pitch your tent on one of the generous spaces on their fields. Let the surrounding moorland breathe a waft of tranquility and fresh countryside air over you.
The Green Escape
Exclusive hire shepherd hut glamping sleeping up to 20 amid six acres of secret woodland in the Surrey Hills.
Whitlingham Broad Campsite
Just a stone's throw from beautiful Norwich, this wonderfully laid back site is your gateway to the famous Norfolk Broads.
Hop Pickers Wood
A secret, shady glade near an old castle... the stuff of fairy tales...
Little Meadow
Bucking the holiday-park trends that abound in this part of the world, Little Meadow remains happily…well, little. Simple, friendly and unassuming, it's a low-impact, ecological hillside site with terraced pitches that offer great views .
The Mains, Eden Valley
Boutique and bijou farm camping in a small orchard and kitchen garden.
Yurts Cornwall, Tremeer Farm
Yurt-tastic, Cornish retreat...A ger-eat place to catch up on your chilling.
High Meadow Yurts
Two wonderfully furnished glamping Yurts, set outside the Brecon Beacons National Park on a traditional working farm.
Manor Farm
No matter where you pitch your tent up, you can always see the stunning Norfolk coastline. You can't get a better holiday than that.
Under the Milky Way
A spacious, eco-friendly glamping site within driving distance of Devon's finest beaches and Exmoor National Park.
The Meadows
A pleasing mix of the traditional, the luxurious and the wild, The Meadows is ideally located to explore Cornwall's idyllic Pentewan Valley.
Gambledown Farm (Feather Down Farm)
Impeccably finished family glamping on a typically welcoming Hampshire farm.
Dot's Camping
If you're hunting that harmonious feeling, then come to Dot's. Pleasant Hampshire countryside matched with a helpful host.
Happy Days Retro Vacations
1950s airstreams decorated with pretty, homely touches, with plentiful fresh, countryside air and wonderful sunsets. Glamping at its best.
Chapelfield Campsite at Sarah's Field
Friendly, back-to-basics site with plenty of space for the kids play. AND it's right in the New Forest!
Badgell's Wood Camping
An immersive camping experience in the beautiful natural setting of an ancient woodland.
Lanefoot Farm
View-tastic camping nestled in the stunning Cumbrian valleys.
The Quiet Site
Sshh, don't tell anyone but this place offers a great escape, supreme walking and caters well for kids too, just don't go shouting about it.
Twitey's Tipis and Camping Meadows
Busy days, peaceful nights. Life's good at Twitey's Tipis...
The Original Hut Company
Relax in the lap of luxury at this romantic, glamping woodland hideaway.
Alde Garden
Small in size, diverse in style. Alde garden will most certainly spoil you!
Inside Out Camping
Crag and fell views, and riverside fun.
Firle Camp
Beautiful green countryside, all around.
Abbey Home Farm
An eco-camping experience in a choice of settings on an organic farm with an award-winning organic shop and café on your yurt-step. Which, of course, is green in colour.
Bedgebury Camping
Lush, wide open, spacious camping with some small touches of finesse thrown in...
Polgreen Glamping
Cornwall's finest surfing beaches, and some of the most dramatic stretches of coast are just a short hop away - so grab your wetsuit or boots and get out there!
Mount Harry Glamping
A private glamping party in the grounds of a quintessentially English country manor house. What more could you want? How about the South Downs and the coastal delights of the south coast on your doorstep?
Petruth Paddocks
A live-and-let-live place where all are welcome. Kids can be kids, campfires are encouraged, music is not a problem and people feel free to wander round making new friends.
Broad Meadow House
Broad? Maybe not by the size of the campsite but certainly by the size of the beauty at this sea view-tastic spot.
Long Valley Yurts
Morrocan, magical, star-gazing, yurt-loving fun. This is a real somewhere-for-everyone glampsite.
Devon Yurt
Get back to nature without giving up your creature comforts. Glamping on the edge of Dartmoor comes with a sleigh bed under the stars, a wood-fired bath with a view and a ride-on tractor for budding farmers.
Hidden Meadows Glamping
Two safari tents, set in 25 acres of private wildflower meadows, peppered with ponds and reed beds.
Scaldbeck Cottage
Traditional and petite camping. And being near the beach, there's plenty of activities, such as spotting a few seals or you could try your hand at sailing.
Clippesby Hall
Safe, comfortable family camping, with plenty of fun-filled stuff to occupy you, that's if the beautiful Norfolk Broads fail to.
Can't decide whether you prefer wood camping or field? Then make your mind up at this site which offers you both.
Tom's Field (Hampshire)
Sometimes you just can't fight your destiny; this is the campsite formerly known as farmland where the owners caved in to campers constant requests and now, for 28 days a year, this field gets to fulfil its wonderful campsite potential
Low Wray National Trust Campsite
Low Wray National Trust campsite sits on the shore of Lake Windermere. It's a small site with two main areas for camping; one set back from the lake in a clearing surrounded by trees, and another tree-scattered spot right on the shore with expansive views across the water.
Cerenety Eco Camping
'Happy eco camping' brags the tagline. And happy is just what you'll feel here if you're eco-minded and wild about camping. Just a short walk from the kiss-me-quick seaside at Bude, at Cerenety tent life is all about being green and taking it easy.
Plush Tents
Boutique woodland camping in beautiful yurts and bell tents at the foot of the South Downs.
Pop-Up by the River
Back-to-nature glamping, camping and riverside campfires in rural West Sussex.
The Withies Campsite
A friendly Shropshire campsite in an Area of Outstanding Natural Beauty on the beautiful Wenlock Edge.
Hollands Wood Camping in the Forest Site
Described by David Bellamy as a "paradise park", Hollands Wood is a campsite where nature is valued more highly than commercialism.
The Little Wild Campsite
A rustic 28 day pop-up site with excellent views and a wild location on the Cornish coast.
Ruthern Valley Holidays
Did you know that aside from beaches, Cornwall has a few woodland treats up its sleeve? Go chase a squirrel or two at this woody retreat.
Usha Gap
For those seeking a traditional North Yorkshire Dales camping experience, Usha Gap is pitch-up perfection.
Hare & Hounds Camping
A small, peaceful campsite with a relaxed atmosphere and an unbeatable location for visiting Rye and East Sussex.
Wardley Hill Campsite
A relaxed, back-to-basics campsite on the Norfolk Suffolk border.
Burnbake Campsite
Seriously chilled out vibes here in this woodland wonderland of a campsite.


text:
'Hunger Games' Fans: Take a Tour of the Capitol! (EXCLUSIVE)
Click the "guide" in the lower left corner and once you login, or create an account, you can then tour the welcome center and even see Seneca Crane's control room.
Seriously– it looks just like the movie! Lionsgate worked with Microsoft to create the Capitol Tour, which is optimized for Internet Explorer 9.
Once you are in the control room you can watch an exclusive clip showing the reaping. Just type "ivolunteer" then press enter and it will appear on your screen.
With Grammy winning band Arcade Fire playing in the background, take a tour around and see if you can find Peeta, Katniss, Effie, and Haymitch!
Want more from the cast? Check out our interviews from the world premiere:


text:
Brian Austin
Hawk and Tom are not wearing pants right now.
Hawk & Tom Morning Show
Hawk Harrison
Tom Steele
Heidi Aiken
Kato Keller
Supa Dave
Ryan Seacrest
Listen Live
Listen on Your Phone
Audio Vault
Photo Gallery
E On The B
Heidi's Blog
B Events
Add YOUR Community/Charity Event
Today's Music News
Yesterday's Music News
Artist Profile
Deal Seekers
B Bling
Upstate Perks
1 Thing
Hit a deer with my Jeep
by Hawk
posted May 12 2010 3:14PM
Well if you haven't heard the latest I hit a deer on my way to work. I was driving down Scuffletown Rd. about 5:15 when all of a sudden I see this deer. I slowed down thinking to myself ......hey.......I almost hit that ddddeeeeerrrr (bam). Yep just as the first one crossed over the 2nd deer was traveling behind. He or she wasn't so lucky. I felt so bad all day long...... I had killed Bambi's Mom. The funny thing is( if there is such a thing when speaking of death) I happen to be listening to the fireflies song by Owl City. That is one of the happiest songs ever making references about nature and all. Since then ever one has told me that if you see one deer watchout because there is probably another following behind. I did find out that if you hit any kind of animal deer, racoon, dog , cat, cow, etc... the insurance company calls it an act of nature and usually you are covered for any damage done to the automobile...... I don't know why the county puts up all those deer crossing signs because the deer read the signs and think its ok to cross here.....Well no it's not if I'm driving.....thanks for reading ...hawk
Tags :
Social :
Back To Blog
Hit a deer with my Jeep
Please Enter Your Comments Below
Title :
Comment :
Please enter the text as it is shown in the box below :
Refresh Image
advertise with us
Recent Posts
Say "Hello" with Classroom Instruments
The Farce Was Missed with this One
There's a 75% Chance of This Dress Showing Up on Your Weather Report
Literally Walks in for the Touchdown
"This Movie Has a Lot of Boxing"
Book Club
Fashion Friday
Save $ this week!
Blog Tag Cloud
No Tags Found !
Nov 2015
Oct 2015
Sep 2015
Aug 2015


text:
Get the latest Flash player
Date: 3 years ago
Runtime: 12:10
Views: 334,045
Raw ass to mouth in the woods (bareback)
After long time we decided to make new video including ass to mouth action, but this time outdoor, in the woods near road, so you can hear cars. We were really horny before this video. My boyfriend start to fingering my tight ass while I was wanking my hard cock and then he started fucking me raw with his nice big cock (02:40). I swear it was so hot moment to fuck outdoor in the woods and a bit risky, because it was near resting place for cars so somebody could come and find us. If you have already seen our "Ass to mouth" video, you know for sure, that I love to suck cock after it has been in my ass. In the woods I was doing it many times so you can enjoy ATM in times: 03:50; 05:10; 06:50; 09:10 (with cumshot/facial); and at 11:00 again. We are waiting for your comments and hope, you will love this video even more than our one year old video Ass to mouth. So please, if you like this outdoor ATM video, let us know. Post comment, rate, add to favourites and click on Feature icon. Thank you!
cum, cumshot, wank, ass, butt, fuck, gay, suck, big, young, blowjob, facial, load, twink, bareback, muscle, wanking, public, deepthroat, outdoor, risky, woods, jekring, atm, a2m
Top Comments
All Comments
damn HOT man - beautiful men - great cock - BIG load - you've got it all guys
so hot, fun & risky
u boys r so fuckin gorgeous
luv the outdoors setting
great camera work & sound
luv raw fuckin and suckin
one of the best outdoors vids ever!
ty 4 sharing
plz make more!
addin to my favs
very hot, men
i love ass to mouth
Hot, guys!
Hot fucking...
love this video may be cos i never had sex in wood mmm
Damn, this is so hot.. You should totally make more of these videos!! (Favorited this one and also the 'one year old ATM' video as well - also subbed... looking forward for more hot vids) Keep up the good work!
ps. Fantastic cock and body of top and the same goes for the bottom, in this case, a fuckable ass
Very hot clip! Agree with the top comment. Love the top's big cock.. and how he plows his bottom. I would get on my knees for that big cock too.
mmm...love a cock after it has probed an ass
so hot sexy i wanna try it
Very exciting !
Awesome video. You guys are the best. And the ass to mouth action is so fucking hot...made me blow a huge load. And to have it outside in the woods....perfection.
damn, damn, damn
that was fucking hot.... would love to do you in the woods as well, while the top does me ;-)
hot fucking session! how can i get on your sessions?
fucing hot *****stars
Nothing nicer than a bottom bitch who loves to lick clean a hard cock that's been pounding and breeding his slutty cunt!! Yea for ass-to-mouth!!!
Thank you!
yeah fuck that ass..very nice and hot
Great video, ass to mouth is so hot ;-)
yeah..excellent..ass to mouth..dirty fukkas..I loved it..
watching this vid full screen is the ultimate....the cum facial is way HOT...and the ATM...mmm....this will go on my wank repertoire for sure....
So Fucking HOT / Raw ! ronnie
So fucking hot, guys! You make really horny!
love ass to mouth too.
THis is just what men fucking should be - ************breeding up the ass. I'd love to come and stick my big massive cock in all those loads and add my strain to them in his hungry shitter
Five stars
Very hot video
I love it !!!! More of this !!!!
The top's cock looks so thick! I would want to enjoy every inch


text:
The SEEN is a growing network of local and regional businesses, organizations, and individuals who recognize that a strong economy depends on the health of our community and natural environment.
See who is in the SEEN → Alphabetical Listing of Businesses and Organizations
Save money while supporting SEEN members through our Discount program!
Ready to join the SEEN?


text:
Cedar Fair appoints chief marketing officer
SANDUSKY -- Cedar Fair LP has named Kelley Semmelroth to the new position of executive vice president and chief marketing officer.
Ms. Semmelroth most recently was senior vice president of marketing at TD Bank and previously was a marketing executive with Bank of America and Walt Disney Parks & Resorts.
Search: Go →


text:
If you are looking for strongly dedicated technicians to help you out with your heating and cooling problems, Adams Heating and Cooling is the right choice for you. They offer services such as air conditioning repair, furnace repair and duct cleaning services. Upon availing of their services, every client can also benefit from their One Year Warranty on all parts and labor.
Adams Heating and Cooling is committed in providing impeccable service to their customers, it is their mission to put every client's comfort at top priority. All their professional service technicians fully understand how your cooling or heating system works so you do not have to worry anymore!
So if you are having troubles with your heating or cooling system, call Adams Heating and Cooling now! They are the experts when it comes to air conditioning repair, furnace repair, duct cleaning services or any other heating or cooling problem you might have!
For more information, log on to http://www.adamsheatingandcooling.com/


text:
The riders have arrived and are ready to battle at the 2014 AWT Pistol River Wave Bash. Day one saw light wind giving competitors an extra day of rest and the forecast looks promising for late Friday through the weekend.
High speed boat ride with Jerry's Rogue Jets
The no wind day gave everyone a chance to experience a jet boat ride up the Rogue River, just at the north end of the town of Gold Beach, Oregon, with Jerry's Rogue Jets. If speeding up the river and sliding through 360-degree banked turns is your thing then this is a must booking on your next trip to the Pistol River area.
Inn of the Beachcomber gives $1,500 cash prize: With no official title sponsor at this event Ted Eady, owner of the Inn of the Beachcomber in Gold Beach, has stepped up in a huge way offering $1,500.00 in prize money for the event. Ted is a huge part of making the Wave Bash a success every year and we thank him greatly. Please support the Inn of the Beachcomber on your next windsurfing road trip along the Oregon/California coast.
Quote from Head Judge Chris Freeman: 'It has been a long couple of weeks for the AWT riders and staff and we are yet to run a windy heat but the wait looks to be almost over. Pistol River normally delivers massive winds and waves but the machine is taking a break today on the first day of possible competition. Have a look at the stacked brackets in all divisions.
In the Pro Division we have three world champions including the current PWA World Champion Marcillio Browne, 2012 and 2013 AWT Champion Camille Juban and 2012 AWT Champion Nathan Mershon. Add to these the best wave riders in the US and an array of foreign talent including the frenetic Antoine Martin and Moroccan magician Boujmaa Guilloul. Don't forget two time Aloha Classic AWT Champion Bernd Roediger and PWA Aloha Classic winner Levi Siver together with Ho'okipa master Morgan Noireaux.
Together these riders make up one of the toughest brackets ever put together for an AWT contest. In light of the depth of talent on display this weekend for the first time the Pro bracket will feature two repechage rounds (#2 and #4). To promote progressive riding riders winning in Round #1 and Round #3 will skip the next round while the others fight it out for the right to challenge. A lot of work has gone into improving all areas of judging in the off season and we are working to increase transparency and help riders improve their performance, the result is already being realized as a number of Amateur riders now recognizing what a difference jumping can make to their score card were seen practicing their Forwards yesterday, with some completing them for the first time.
Back from injury reigning Women's Champion Ingrid Larouche will be fighting strong competition from rising teenage star Fiona Wylde and big wave magnet Sarah Hauser of New Caledonia. The Soccer World Cup might be starting today but be sure not to miss any of the AWT heats as there is going to be some insane action, huge upsets, massive crashes, tears and joy in the frigid Oregon waters this weekend.'
AWT website


text:
Mundfickstute - Deepthroat-Training
Tags: deep throat shemales
Really Nice Deepthroat Training
Tags: amateur blowjobs deep throat matures
Tags: amateur
Tags: double penetration latex
sissy training
Tags: shemales
Tags: anal ass bisexuals cum fetish fitness gym
geisha training
Tags: asian
anal training
Tags: anal toys
Training Wheels
Tags: black ebony teens
Sissy Training
Smother training
Tags: big tits deep throat face sitting femdom hairy pussy smothering
Girlfriend Training
Tags: amateur girlfriend
Puppy Training
Tags: amateur anal ass bdsm bondage fetish group sex
Obedience Training
Tags: amateur bizarre bondage feet femdom fetish hairy
bareback training
Tags: gays
Hardcore Training
Tags: fetish hardcore
Sensitivity Training
Tags: blondes blowjobs doggystyle hardcore stockings
Personal Training
Tags: anal babes blowjobs hardcore
Anal Training
Tags: anal
Asshole training
Tags: anal asshole blowjobs couple gays oral
Sexual Training
TrafficHolder.com - Buy and Sell Adult Traffic|Trafficshop.com - Let your business grow|Contact Us|Webmaster
All content, including images, text, graphics, video and audio content contained in the website is protected under the laws of copyright, owned or under license to company or its designees,and represents proprietary and valuable intellectual property.
Contact Webmaster 18 U.S.C. 2257 Record-Keeping Requirements Compliance Statement
Powered by Stream Rotator


text:
|A lubrication analysis of pharyngeal peristalsis: application to flavour release.
MedLine Citation:
|PMID: 20816856 Owner: NLM Status: In-Process
|After eating a liquid or a semi-liquid food product, a thin film responsible for the dynamic profile of aroma release coats the pharyngeal mucosa. The aim of this article was to analyse the fluid mechanics of pharyngeal peristalsis and to develop a simple biomechanical model in order to understand the role of saliva and food bolus viscosity on the coating of pharyngeal mucosa. We began by analysing the physiology and the biomechanics of swallowing in order to determine relevant model assumptions. This analysis of the literature clarified the types of mechanical solicitations applied on the food bolus. Moreover, we showed that the pharyngeal peristalsis in the most occluded region is equivalent to a forward roll coating process, the originality of which is lubrication by a film of saliva. A model based on the lubrication theory for Newtonian liquids was developed in dimensionless form. The parametric study showed the strong influence of relative saliva thickness on the food bolus coating. A specific experimental device was designed that confirms the model predictions. Two sets of conditions that depend on the relative thickness of saliva were distinguished. The first is characterised by a relatively thin film of saliva: food bolus viscosity has a strong impact on mucosa coating. These phenomena are well represented by the model developed here. The second is obtained when the saliva film is relatively thick: hydrodynamic mixing with saliva, interdiffusion or instabilities may govern mucosa coating. Finally, these results were extrapolated to determine the influence of food bolus viscosity on the dynamic profile of flavour release according to physiological parameters.
|Clément de Loubens; Albert Magnin; Eric Verin; Marion Doyennette; Ioan Cristian Tréléa; Isabelle Souchon
Related Documents :
|2324436 - When it's hard to swallow. feeding techniques for dysphagia management.
1057016 - Periodontal considerations in operative dentistry.
22347616 - Combined fruit and vegetable intake is correlated with improved inflammatory and oxidan...
22833556 - Does the school food environment influence the dietary behaviours of norwegian 11-year-...
18926606 - Forty years of atmospheric radiocarbon monitoring around bohunice nuclear power plant, ...
25063856 - Mitochondrial uncoupling as a regulator of life history trajectories in birds: an exper...
Publication Detail:
|Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-15
Journal Detail:
|Title: Journal of theoretical biology Volume: 267 ISSN: 1095-8541 ISO Abbreviation: J. Theor. Biol. Publication Date: 2010 Dec
Date Detail:
|Created Date: 2010-10-25 Completed Date: - Revised Date: -
Medline Journal Info:
|Nlm Unique ID: 0376342 Medline TA: J Theor Biol Country: England
Other Details:
|Languages: eng Pagination: 300-11 Citation Subset: IM
Copyright Information:
|Copyright © 2010 Elsevier Ltd. All rights reserved.
|INRA, UMR 782 Génie et Microbiologie des Procédés Alimentaires, CBAI, 78850 Thiverval Grignon, France. email@example.com
Export Citation:
|APA/MLA Format Download EndNote Download BibTex
|MeSH Terms
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Disambiguation in the biomedical domain: the role of ambiguity type.
Next Document: Quantifying the interaction structure and the topological importance of species in food webs: a sign...


text:
Social Media jobs
Found 5 jobs for £45,000 - £55,000 matching your criteria
Order by: Newest first Salary
Client Services Director - Upto £60,000 plus visa sponsorship and bonus
- Sydney (Region), New South Wales (AU)
- Upto £60,000 plus visa sponsorship and bonus
How would you like to work "down under" - swapping the streets of England for a sandy beach in Australia? Wouldn't you love the opportunity to beco...
Digital Marketing Manager, London
- London
Well-rounded Marketer, with a background gained in the wine / spirits sector, sought by this high-end drinks retailer
Business Development Manager - Upto £40k plus commission
- Brighton, East Sussex
- Upto £40,000 with commission
Our client is among the world's leading Digital Marketing Agencies, with the objective to improve the performance of global websites and online pla...
SEO Manager - Upto £45k
- Hertfordshire (23 minutes train journey from Central London)
- Upto £45,000 DOE
Based outside of London, this robust, fast growing Digital Marketing Agency is just a 23 minute journey from London's Marylbone station. Based in t...
eCommerce Manager - Upto £50k DOE
- Just outside of London
- Upto £50,000 DOE
Based just outside of London, our client is a Leading Online Retailer in Luxury Motorcycle apparel. With over 250,000 customers on their database, ...


text:
We help to rethink public services, build seamless connections and put vital information in the hands of those who need it.
We help housing providers make the right decisions today and plan effectively for the future.
We are helping police forces to revolutionise collaboration, transform productivity and protect communities as part of the Athena programme.
We help to streamline eligibility processes, speeding up applications for Blue Badge parking, housing benefit and emergency welfare payments.
We run health registries and screening programmes that help people to get the treatment they need fast and to stay fit for longer
We'd like to make it as easy as possible for you to get a response enquiry.
For general enquiries about our solutions and services the best way is to submit the email contact form below. this way we can get the right person to give you call back. We will normally be able to get back to you on the same day, but if not, we will aim to get back to you on the following working day.


text:
Californication: Season 5, Episode 11
The Party (25 Mar. 2012)
Ratings: 8.2/10 from 508 users
Reviews: write review
Hank has finally overstayed his welcome at the house of Karen and Richard Bates, so it's time to hit the road. But not before one last stop at Malibar, where he runs into Lizzie, who uses ... See full summary »
Director:Michael Lehmann
Writers:Tom Kapinos (creator), Tom Kapinos


text:
Lol, just lol.
I want to play 2 player coop zombies with my friends online, but to be able to do that we both need 2 controllers. we need to join a online game, sign off with the xtra ones right before the game starts to be able to just play with 2.
No, im not playing custom games as it doesnt count on your leaderboard round ranking.
Just find a split screener and ask them nicely to back out on the last 2 seconds before game starts up.
Although, I will agree with you. It would be so nice if Treyarch would allow for a selection of 2, 3 or 4 player co-op selection. I also think you should have a check mark box that only links you to other people with microphones because seriously people you cannot play this game right without a mic..
Rank selection/preference would be nice as well but this would never happen!
hahahah that's too easy...right...
}{appy }{unting!!
Here we go agin thanking trasharch cares what it's customers thank/ ask for good luck.


text:
Singapore Botanic Gardens Excursion Reviews
Lose yourself in the lush greenery and vibrant assortment of plant life in the 20-plus-acre playground for nature lovers, known as the Singapore Botanic Gardens. There's a rainforest, a lake and all manner of grounds -- some showcasing things like medicinal herbs
1 review
Jan 2013
Beautiful orchidsRead Mezzakat's full Celebrity Millennium cruise review
Provider: Celebrity Cruises
2 reviews
Jan 2013
Very nice and the tour of the city was wonderful.
Provider: Celebrity Cruises
L. A. McDaniel
1 review
Nov 2012
Botanic Gardens were magnificent, especially the orchid exhibit, which does charge admission.
Provider: Celebrity Cruises
Sponsored links
More Singapore Information


text:
Race & Spider-Man: Sony Confirms a Glass Ceiling for Miles Morales
View Single Post
May 16 2014, 06:14 AM
Rear Admiral
Location: On the run.
Re: Race & Spider-Man: Sony Confirms a Glass Ceiling for Miles Morales
Mr Light wrote:
How about a Peter Parker starring movie that introduces Miles Morales as his partner?
, if there ever was one, I'd rather keep them separate...but I can see the appeal of that approach.
How about a rebooted Spidey series where Parker Parker (as an already well-known Spider-Man, ie: no origin story needed) dies very early on in the first film and inspires the newly powered Morales to take up the mantle? ...Or I can see that back-firing if fans rage against being tricked into seeing a Spidey movie "where he's barely in it".
View Public Profile
Send a private message to Tosk
Find More Posts by Tosk


text:
Kidsday meets Selena Gomez
Former Disney Channel star Selena Gomez talks to Kidsday about her many dogs and her clothing line. Videojournalist: Patrick Mullooly (Jan. 20, 2013)
Newsday.com now uses Facebook for our comment boards. Please read our guidelines and connect your Facebook account to comment.
Good Morning LI: It's National Eat a Red Apple Day! Gillibrand: 'FEMA has failed to do its job' Good Morning LI: It's Cyber Monday! Learning to be grateful for wildlife - even opossums Police seek thief who took PlayStation Brentwood man killed, second man wounded in shooting Two injured in early morning fire at North Babylon house Marchers rally for action on climate change in Manhattan North Babylon mother killed crossing Southern State Parkway after surviving accident Local retailers talk Small Business Saturday 2 dead in fiery truck-car collision in Inwood Black Friday protest


text:
Support our Indie go go campaign for a Parklet outside Saul's!
The parklet will be public space for all to enjoy.
We're selling gift cards at discounts we've never offered before and won't be able to again.
You can pitch in by scooping up deals on Saul's gift cards, and letting all your friends know about this chance to build a parklet.


text:
Come and celebrate Swiss National Day with us!
Saas-Fee, 1. August
Beside folkloristic performances of some associations of Saas-Fee as the folkloristic dance group, the alphornfriends of the glacier village, the yodelling club Gletscherecho, the Music of old times and the wind orchestra Alpenrösli a music band will be setting the mood and invite for a swinging evening.
Saas-Grund, 1. August
It's already the 45rd street-parade with the motto "Life in the olden times in the Saas Valley". Over 35 very impressively decorated floats display the customs and traditions of the people of the Saas Valley.
Saas-Balen (31. July)
And also in Saas-Balen the traditional festivities will take place. Please note: the festivities in this community will already take place on the 31 July.
Alter Dorfplatz
3910 Saas-Grund


text:
Lifetree Cafe describes itself as a "conversation cafe" focusing on thought-provoking topics and respectful dialogue relating to life and faith.
The nonprofit group meets from 5 to 6 p.m. Saturdays at Francis Family YMCA, 2000 W. Dean Rd. The free, local group is sponsored by New Life Community Church of Toledo.
- Topics include: Saturday: "Coping With Grief: Finding Your Path to Peace."; July 27: "UFOs: Chilling Tales from the Most Abducted Man." For information, go to lifetreecafe.com or facebook.com/LifetreeCafeToledo.
The Francis Family YMCA will host a Day Camp Carnival from 10 a.m. to noon July 30.
Outdoor games and a cake walk inside the facility will be held.
Tickets are 25 cents for each game or five tickets for $1. Proceeds will go to the YMCA Scholarship fund.


text:
she just downed another ounce or two of vitamin d
Thanks for helping us associate the correct Pornstars to this video!
Help Us: Do these Pornstars appear in this video?
Pornstar Name:
Thanks for helping us sort this video!
Help Categorize This Video
Help YouPorn show the right videos when you search by categorizing even more videos!Play Next Video
Hidden camera video of sexy wife masturbating with her fingers deep to orgasm.
All Comments (43)
she just downed another ounce or two of vitamin d
Would love to she her fist a woman like this.
she can rub and finger me like that any time, and I will return the favor. Very beautiful
Beautiful and sexy, thanks!
jaime les voir se masturber comme sa c trop kiffant surtout de les entendre, entendre la mouille
i hope the person taping this got some after she warmed it up. Seems like she would be a great fuck, i would do her if i could.
Super hot chick, who is she? Wish we could see her face when she cums
That is one sexy wife, her body is awesome, too bad she hasn't to do all the work herself! Little help next time?
MMMmmm, delicious. I'd give her several more orgasms with my mouth, fingers, tongue and cock.
slap your thang some more for us
Delicious!! More please!
That was great and it is also to bad that a fine looking lady has to satisfy herself but it appears she is used to having a big dick as she was working three fingers inside her pussy so she has to be used to a thick dick what do you think?
What a nice looking wife to bad her husband does not take care of that fine looking pussy I would love to help her achive an orgasm without her having to do it by herself.
that's a lot of fingers in her pussy but it sounds tight and wet, bet its hot too. this girl knows how to make herself feel good.
Now this woman knows how to get off. Nice tight pussy! Wish we could see her face too.
Really hot and nasty, shes definitely a milf i'd fuck
Awesome, beautiful woman, very sexy!
I want to get up on that wet hot momma, you can hear her pussy cum on her fingers
We caught our mother enjoying solo sex by watching her from the balcony. We have been irritated for several days.
Very sexy woman, nice moves, hot body
I so want to fuck this guy's wife, I could make her cum 10 more times
eine schöne Muschi Fingern tief würde gerne ficken
Hey naughty wife, hot fisting, join us sometime!
Her toes curl when she cums, very sexy feet, wish I could jack off and cum on her feet while she fingers herself
Really sexy! Did she really not know she was being videotapped? I loved seeing her fingers go deep!
can I eat your pussy
You can hear how wet she is while she fucks herself, great tits and intense orgasm, let's see more of this milfy.
I want a wife like this!! Hot body and loves to rub her slit and finger fuck for the camera? Where do I place an order?
Gorgeous big dark nipples and natural breasts. I'll bet she'd enjoy having those sucked while her man fucks her.
I want to suck the cum out her sexy cunt now that she's all hot!
Hot! Would she let me lick it now that its all wet with her cum?
what tempo is that ? allegretto or allegro?
Awesome soft tits, great frigging too.
Really good quality for a hidden camera. She's stunning and sexy! Wish we could see more and up close.
Delicious pussy want to taste that all wet and hot
I like her pussy and how she gets her fingers in there
Busted! Girls do it too! Really hot to sneak a peak of her playing.
I love watching my wife do this to her pussy and i love to join in when she doing herself like this
This is sooo hot, I hope its real, makes me want to jerk it
this is great because its not so up close that it looks luke a gyno exam. I feel like a voyeur who is spying on her from the door or window, and it make me excited
Hot and she has a great bod!


text:
iHerb Customer Reviews
Customer Ratings
Reviews 4-star
Sort By
Can't really tell
Posted by Reviewer2860699 on Nov 14, 2012
Since I take so many supplements, I am not always sure what each of them contribute to my overall excellent health. My wife takes this regularly and says it helps her.
Was this review helpful to you?
Note: iHerb does not imply any medical claims from this review. Disclaimer.


text:
Ski resorts Lueneburg Heath
Best rating:
4 out of 5 stars
Rated by Skiresort.info, the world's largest test portal for ski resorts.
Click here for rating process info
Skiresort.info presents the best ski resorts for the family ski holiday in the Lueneburg Heath.
The offering for families and children is very large at the Snow Dome Bispingen. The main... more


text:
Bump for Pets :)
Let's all stop in and show our pride for our pets!
You can bump with pics, words or just hit the bump button... I want to see how much you love your pets :)
My three ferrets are in the 2014 calendar Ferret's North Information & Rescue Society put together. They included all the ferrets' pictures that were sent in this year for the photo contest. I love my guys!!
Good morning, sunshine!


text:
The H1N1 flu virus and your student – Part Two
So the Quebec government has turned down McGill's request to hold a vaccination clinic on campus. Does that mean my child cannot get vaccinated against H1N1? No it doesn't!
The provincial vaccination campaign got underway on October 26 according to a specific roll-out schedule. This vaccination sequence was determined using guidelines established by federal public health authorities to ensure optimal protection for those people who run the greatest risk of developing complications and requiring intensive care if they catch the virus.
Vaccination: where and when – and is it available to all?
The H1N1 flu vaccine will be made available to all Quebec residents, at various locations in and around the island of Montreal. All students will have access to the H1N1 flu vaccine through the Quebec Government's vaccination campaign. Here's how it works:
According to the Quebec government's schedule, students who are 20 and over, do not have a chronic disease nor care for a child less than 6 months of age will have to wait until December 7 to get vaccinated. The vaccine will be offered free of charge at vaccination sites across Quebec. Do note that this schedule has been amended several times, so it is important to go to the source to make sure it is accurate. The list of Montreal vaccination centres, published by the Ministry of Health, and the current roll-out schedule are available at www.santemontreal.qc.ca/en/.
As of November 12, the roll-out schedule states that your child will be able to receive the H1N1 vaccine according to the following sequence:
- If your student has a chronic medical problem such as asthma or diabetes or cares for a child less than 6 months of age: right away
- If your student is 19 or under without a medical condition: as of November 19
- If your student is 20 or over without a medical condition: as of December 7
Which documents does my child need to bring and what will it cost?
Out-of-province students who are covered by another provincial health insurance plan must present both their provincial health card and proof of residence* in Montreal. The vaccine is free of charge.
International students must present both CAQ/Student Permit and a proof of residence* in Montreal. The vaccine is free of charge.
Students not covered by a provincial health insurance plan, or, studying as an international student may be required to pay for the vaccine out-of-pocket (approximately $15-$20). These students must also provide proof of residence* in Montreal.
*Proof of residence can be in the form of a lease or a utility bill, and can also be obtained by clicking the Personal tab on Minerva
Where are the vaccination centres located and which is closest to campus?
Currently, the two clinics that have been announced and that will be accessible to our student population residing in the downtown core are:
Plaza Alexis Nihon, 1500 Atwater Avenue, metro level, Atwater metro (green line).
This clinic is currently in operation;
Palais des congrès, 1001 place Jean-Paul-Riopelle, metro Place-d'Armes (orange line).
This clinic is set to open on November 16.
Both clinics are to be open 7 days a week, from 8:00 a.m. to 8:00 p.m. They each are approximately 25 minute walk from campus or some 15 minutes away by public transportation.
For the most up-to-date information of vaccination centres as well as other clinics on the island of Montreal, please visit www.santemontreal.qc.ca/en/.
Note that the delivery process of the vaccine has been greatly improved by a wait-line management system. Currently, this means coupons are distributed each morning to people arriving at vaccination centres; each coupon corresponds to a time slot that same day when the recipient is asked to come back to get vaccinated, hence cutting down on waiting time. This means student won't have to waste a whole day sitting in a waiting room and can instead return to class or to their daily tasks.
What should my student do in the meanwhile?
The Ministère de la Santé et des Services sociaux reminds Quebecers that in 90-95% of cases, people who catch the flu do not need to see a doctor and can take of themselves very well at home. To help citizens protect themselves and others, the government has issued a Self-Care guide which is available here.
What you can do as a parent is stress the importance for your student to receive the H1N1 vaccine. You can also direct your student toward McGill resources, as well as the latest information released by local health officials, presenting the most up-to-date information on where to get vaccinated. There is also a new website featuring physicians from the McGill University Health Centre and which uses the latest in online communication technology to help you and your student understand and better fight the spread of H1N1. The six clips currently available on this site present pertinent information in a way that is very accessible and easy to understand, particularly for the digital generation! More information is also available at www.fightflu.ca.


text:
a list of important American figures, including Kim Kardashian, Paris Hilton, Mel Gibson, Ashton Kutcher,
This content community relies on user-generated content from our member contributors. The opinions of our members are not those of site ownership who maintains strict editorial agnosticism and simply provides a collaborative venue for free expression.
All content copyright 2015, The Above Network, LLC.


text:
104 minutes... it would be nice if the useless banter in between topics could be slashed out.
Kreedz Climbing is a unique, non-violent, puzzle-type, 3D-First Person View game where the player attempts jumping and 17 other disciplines like shooting flare targets, racing vehicles, surfing, bungee, bunnyhop, longjump, or sliderace until he/she reaches the top (or end) of any given environment. In Kreedz Climbing no map is the same and can vary with any combination of these skills. It's a race against time and against other players with any discipline in the game.
William is joined by Hectic Glenn and Bluewolf72. They talk about Team Fortress 2's War!, L4D2 SDK, Orion, Overheid, City 17: Episode 1, Black Mesa, SHIFT, Visage, Return to Church, Post Script and more…
Posted by William on Dec 15th, 2009
(Special thanks to the Overgrowth team and Iiro Jäppinen for providing these nice icons!)
104 minutes... it would be nice if the useless banter in between topics could be slashed out.
Yeah, should slash only some, but NOT all.
@Podcast 17
Thanks to them that they noticed RTC and talked about it, it was nice to hear what they think and talk about it.
It's great to know that RTC is being noticed by more and more people also thanks to Podcast 17 more people will find this mod. :)
I enjoy the banter, however I feel it would be nice if they included the extended show notes and the related links along with the ModDB post, instead of having to go to their site to get it.
We used to do that, but then we found that it cluttered the moddb post and fewer people really wanted to listen to the show. We figure those people who want to see the extended show notes won't mind clicking an extra link in another window to read them. I hope this isn't too much of an inconvenience... maybe I can put one of those "more+" links that expand and contract for convenience. Hmm... /me has an idea.
Banter is the best bit. =D
been dying for this weeks edition
i love it ^_^
Still working up something to email to valve...
THERE MY BLOODT HEROS. There level design is just shear brilliance.
I hope one day i can reach the skill level to be able to work for them.
Orion is not worth playing , 2 players online right now , its a big disapointment considering it took so many year's to make , imo this mode is a big faliure , sorry to say it
Desura will probably change that. Haven't tried Orion but from what I've seen it looks good enough for the Moddb-team to release it on the desura platform.
Just because people aren't playing it doesn't make it a failure. The game is pretty solid and patch 2.0 will be pretty good imo. Tune in this week to hear the full story.
mode = mod
Woo KZMod was announced for a December 21st release. Glad that you guys talked about it.
We'll be touching on it again this week.
About Overheid: there are two maps included, and they're from what is basically a "crescendo event", to use L4D terminology.
It's really just a small taste in the demo, but it gives you an idea of what else is coming. Check it out.
Overheid: Moddb.com


text:
July 28, 2014
Maurice Wingate
Kevin Ollie (UConn) and Eddie Jordan (Rutgers) had their eye on a promising NY forward that played for a less recognizable team in Vegas in the Fab 48. ...More... To continue reading this article you must be a member. Sign Up Now!
Already a member? Click here to sign in


text:
1The word of the Lord that came to #(2 Pet. 1:21)Jer. 26:18Micah of Moresheth in the days of #2 Kin. 15:52 Chr. 27:1–9Is. 1:1Hos. 1:1Jotham, Ahaz, and Hezekiah, kings of Judah, which he saw concerning Samaria and Jerusalem.
The Coming Judgment on Israel
2Hear, all you peoples!
Listen, O earth, and all that is in it!
Let the Lord God be a witness against you,
The Lord from #(Ps. 11:4)His holy temple.
3For behold, the Lord is coming out of His place;
He will come down
And tread on the high places of the earth.
4#Amos 9:5The mountains will melt under Him,
And the valleys will split
Like wax before the fire,
Like waters poured down a steep place.
5All this is for the transgression of Jacob
And for the sins of the house of Israel.
What is the transgression of Jacob?
Is it not Samaria?
And what are the #Deut. 32:13De 33:29Amos 4:13high places of Judah?
Are they not Jerusalem?
6"Therefore I will make Samaria #2 Kin. 19:25Mic. 3:12a heap of ruins in the field,
Places for planting a vineyard;
I will pour down her stones into the valley,
And I will #Ezek. 13:14uncover her foundations.
7All her carved images shall be beaten to pieces,
And all her #Hos. 2:5pay as a harlot shall be burned with the fire;
All her idols I will lay desolate,
For she gathered it from the pay of a harlot,
And they shall return to the #Deut. 23:18Is. 23:17pay of a harlot."
Mourning for Israel and Judah
8Therefore I will wail and howl,
I will go stripped and naked;
#Ps. 102:6I will make a wailing like the jackals
And a mourning like the ostriches,
9For her wounds are incurable.
For #2 Kin. 18:13Is. 8:7it has come to Judah;
It has come to the gate of My people—
To Jerusalem.
10#2 Sam. 1:20Tell it not in Gath,
Weep not at all;
In Beth Aphrah
Roll yourself in the dust.
11Pass by in naked shame, you inhabitant of Shaphir;
The inhabitant of Zaanan does not go out.
Beth Ezel mourns;
Its place to stand is taken away from you.
12For the inhabitant of Maroth pined for good,
But #Is. 59:9–11Jer. 14:19Amos 3:6disaster came down from the Lord
To the gate of Jerusalem.
13O inhabitant of #Josh. 10:32 Kin. 14:19Jos 18:14Is. 36:2Lachish,
Harness the chariot to the swift steeds
(She was the beginning of sin to the daughter of Zion),
For the transgressions of Israel were #Ezek. 23:11found in you.
14Therefore you shall #2 Sam. 8:2give presents to Moresheth Gath;
The houses of #Josh. 15:44Achzib shall be a lie to the kings of Israel.
15I will yet bring an heir to you, O inhabitant of #Josh. 15:44Mareshah;
The glory of Israel shall come to #2 Chr. 11:7Adullam.
16Make yourself #Job 1:20bald and cut off your hair,
Because of your #Lam. 4:5precious children;
Enlarge your baldness like an eagle,
For they shall go from you into #2 Kin. 17:6Amos 7:1117(Mic. 4:10)captivity.
Loading reference in secondary version...


text:
Program Rankings for Engineering Degrees
Going to a top engineering school can significantly increase your chances of landing a job after you graduate. At all colleges, engineering students take classes in design, math, physics, and related areas, but at top programs, you'll not only have access to the best professors in the world, but you'll also have the opportunity to complete internships with top engineering firms, access to alumni networks and students services, and more. Here are some of the top schools for engineers:
Massachusetts Institute of Technology
MIT is the top school in a number of math and science fields, and for engineers, it is ranked #1 by U.S. News and World Report, as well as highly regarded by a number of other publications. Specifically, MIT is great for chemical, aerospace, electrical, materials, and mechanical engineers, as it is ranked first in all of these specialties. They also have top programs in nuclear and biomedical engineering. At MIT, students have access to 20 different research centers, and if you're interested in coming to engineering from another field, you can enter their part-time Career Reengineering Program.
Stanford University
Like, MIT, Stanford is a top-ranked engineering school according to U.S. News and World Report. This is a slightly larger campus than you'll find at MIT, and they rank #1 for computer and environmental engineering, as well as in the top five for industrial, materials, mechanical, civil, electrical, chemical, and aerospace engineering. They have 65 centers and labs for engineering students to do research at this school, and they're committed to getting women in the classroom at Stanford, even though engineering is traditionally a male-dominated field. Stanford also offers a China Internship Program, where engineer students in certain specialties can study abraod for a semester in an intense internship experience.
University of California at Berkeley
Berkeley is a great engineering education option if you want to attend a top school but don't have the money for a private school like MIT or Stanford. This college has top-ranked programs in the field of environmental engineering, computer engineering, and chemical engineering, and top five programs in a number of other specialties, yet tuition is less than a third of the cost of private school. They have 40 different research centers on campus to help you complete your education, as well as flexible degree options for graduate students.
Guide to Engineering Degree Online Programs
|BS - Electronics Engineering MS - Electrical Engineering
|DeVry University – DeVry University, a highly-regarded leader in online education, offers two Engineering degree programs. These programs combine industy-driven curriculum and hands-on exercises and assignments to give students the skills and knowledge they need to successfully become Electrical Engineers or Electronic Technicians.
|Master of Civil Engineering
|Norwich University – Norwich University prides itself on its commitment to excellence in providing education that follows contemporary techniques and standards. The industry-driven curriculum allows students to successfully fill high-demand positions upon graduation. Students can take online course in order to obtain a Masters of Civil Engineering degree.
|MS in Civil Engineering
|NJIT – New Jersey Institute of Technology is backed by years of experience in helping students reach their career goals by providing online and campus degree programs. The MS in Civil Engineering degree uses industry-driven curriculum and hands-on assignments to engage students in their education.
|MBA – Engineering Management
|Northcentral University – Northcentral University is a provider of several online, accredited degree and diploma programs that focus on bringing flexibility and convenience to students. These programs can be completed from home, school, or even on-the-go. The engineering degree available from NU allows students to fill management positions in the engineering and technology fields.


text:
WASHINGTON - The new pollution rule the Obama administration announces Monday will be a cornerstone of President Barack Obama's environmental legacy and arguably the most significant U.S. environmental regulation in decades.
WASHINGTON - In a major anti-pollution ruling, the Supreme Court on Tuesday backed federally imposed limits on smokestack emissions that cross state lines and burden downwind areas with bad air from power plants they can't control.
WASHINGTON — A bipartisan Senate proposal to expand background checks for gun buyers gained the backing of one Republican and the potential support of a second Sunday as sponsors said the vote expected this week was too close to call.The plan would "strengthen the background check system without i...
WASHINGTON - Energy companies bid hundreds of millions of dollars Wednesday to explore for oil and natural gas beneath 1.8 million acres in the western Gulf of Mexico, while looking forward to the possibility of future drilling in federal waters now off-limits....
WASHINGTON - Four of the nation's largest homebuilders have agreed to pay $4.3 million in fines for failing to control runoff at construction sites in 34 states and the District of Columbia, the Environmental Protection Agency and Justice Department announced Wednesday....
Subscriber Services:
Digital Network:
Social Networking:
Savannahnow.com, Savannah Morning News ©2015. All Rights Reserved.


text:
This document describes how GATK commands are structured and how to add arguments to basic command examples.
Commands for GATK always follow the same basic syntax:
java [Java arguments] -jar GenomeAnalysisTK.jar [GATK arguments]
The core of the command is
java -jar GenomeAnalysisTK.jar, which starts up the GATK program in a Java Virtual Machine (JVM). Any additional java-specific arguments (such as -Xmx to increase memory allocation) should be inserted between
java and
-jar, like this:
java -Xmx4G -jar GenomeAnalysisTK.jar [GATK arguments]
The order of arguments between
java and
-jar is not important.
There are two universal arguments that are required for every GATK command (with very few exceptions, the
clp-type utilities),
-R for Reference (e.g.
-R human_b37.fasta) and
-T for Tool name (e.g.
-T HaplotypeCaller).
Additional arguments fall in two categories:
Engine arguments like
-L (for specifying a list of intervals) which can be given to all tools and are technically optional but may be effectively required at certain steps for specific analytical designs (e.g. the
-L argument for calling variants on exomes);
Tool-specific arguments which may be required, like
-I (to provide an input file containing sequence reads to tools that process BAM files) or optional, like
-alleles (to provide a list of known alleles for genotyping).
The ordering of GATK arguments is not important, but we recommend always passing the tool name (
-T) and reference (
-R) first for consistency. It is also a good idea to consistently order arguments by some kind of logic in order to make it easy to compare different commands over the course of a project. It's up to you to choose what that logic should be.
All available engine and tool-specific arguments are listed in the tool documentation section. Arguments typically have both a long name (prefixed by
--) and a short name (prefixed by
-). The GATK command line parser recognizes both equally, so you can use whichever you prefer, depending on whether you prefer commands to be more verbose or more succinct.
Finally, a note about flags. Flags are arguments that have boolean values, i.e. TRUE or FALSE. They are typically used to enable or disable specific features; for example,
--keep_program_records will make certain GATK tools output additional information in the BAM header that would be omitted otherwise. In GATK, all flags are set to FALSE by default, so if you want to set one to TRUE, all you need to do is add the flag name to the command. You don't need to specify an actual value.
This is a very simple command that runs HaplotypeCaller in default mode on a single input BAM file containing sequence data and outputs a VCF file containing raw variants.
java -Xmx4G -jar GenomeAnalysisTK.jar -R human_b37.fasta -T HaplotypeCaller -I sample1.bam -o raw_variants.vcf
If the data is from exome sequencing, we should additionally provide the exome targets using the
-L argument:
java -Xmx4G -jar GenomeAnalysisTK.jar -R human_b37.fasta -T HaplotypeCaller -I sample1.bam -o raw_variants.vcf -L exome_intervals.list
If we just want to genotype specific sites of interest using known alleles based on results from a previous study, we can change the HaplotypeCaller's genotyping mode using
-gt_mode, provide those alleles using
-alleles, and restrict the analysis to just those sites using
java -Xmx4G -jar GenomeAnalysisTK.jar -R human_b37.fasta -T HaplotypeCaller -I sample1.bam -o raw_variants.vcf -L known_alleles.vcf -alleles known_alleles.vcf -gt_mode GENOTYPE_GIVEN_ALLELES
For more examples of commands and for specific tool commands, see the tool documentation section.
A new tool has been released!
Check out the documentation at CommandLineGATK.
I'm not sure why it hadn't occurred to us to do this before, but we've finally done it: an FAQ article that formally explains how GATK commands are structured, what are the basic types of arguments, and how to string them all together.
We realized that command structure requirements can be confusing, if you are new to command line programs, if only because so many toolkits use fairly different ones. For example, Picard tools (which are also developed at the Broad!) have separate jar files for each tool in the toolkit, while GATK has one jar file containing all the tools. The Picard syntax for passing argument values is also different; they use
= to join the argument name and value, while GATK commands just take a space.
So if that's something you need help with, check out the doc! We'd love to hear from people who are new to GATK about whether this is helpful and how we can improve it further.
Because the thread for distribution of custom walkers is retired and I found another thread that does not help me in the issue that I have, I would like to ask this question again.
I'm planning to write custom walkers for the GATK public framework (using a Maven dependency with jitpack.io) and distribute that software including the custom walkers as a jar file with dependencies, where the only available walkers from the command line will be the custom ones. Although in the logging for my command line will be the version of the GATK framework in use, I would like to use a different logging for the beginning of the program and the error (instead of the link to the GATK webpage), so I will need to create and/or extend classes from the GATK engine (for the command line).
Of course, the software will be open source (MIT license), but I wonder if it could be possible to distribute a software in such a way. In addition, I would like to know if it is possible for the developers to made a tutorial on how to start the GATK engine with a custom walker without using the GATK command line.
Thank you very much in advance.
Hi, I'm trying to extract a few samples from a large VCF file with many samples using SelectVariants and I keep running into this error.
Command: java -Xmx4g -jar ~/GenomeAnalysisTK.jar -T SelectVariants -R /Volumes/odin/reference/16484/mafa5/mafa5. -V 16557.all.exons.mafa5.ann.vcf.gz -o 16580.mafa5.M1M1.vcf -sn CY0320 -sn CY0321 -sn CY0322 -sn CY0323 -sn CY0324 -sn CY0325
at java.nio.ByteBuffer.allocate(ByteBuffer.java:334)
at htsjdk.samtools.reference.IndexedFastaSequenceFile.getSubsequenceAt(IndexedFastaSequenceFile.java:195)
at org.broadinstitute.gatk.utils.fasta.CachingIndexedFastaSequenceFile.getSubsequenceAt(CachingIndexedFastaSequenceFile.java:329)
at org.broadinstitute.gatk.engine.datasources.providers.LocusReferenceView.initializeReferenceSequence(LocusReferenceView.java:150)
at org.broadinstitute.gatk.engine.datasources.providers.LocusReferenceView.
Any thoughts as to why this might be happening?
I am receiving the following error. I am working with SAM files that were exported from CLC, then edited with Picard-tools to addReadGroups. I am not sure if I need to add an additional step to solve this problem, I cannot find any documentation regarding this error.
Please let me know what I need to do to correct this issue.
Thank you!
gatk -T HaplotypeCaller -R spinach_assembly-repeatdetect_PACBIO_V1.3_formated_60.fa -I .sam.list -drf DuplicateRead --alleles Unfiltered_Spinach_PacBio_Reseq_12_Geno_Assay_SNP.fixed.noblanks.vcf --genotyping_mode GENOTYPE_GIVEN_ALLELES --output_mode EMIT_ALL_SITES -o output_raw_unfiltered_spinach_snps_gbs.vcf
INFO 14:48:44,450 HelpFormatter - ---------------------------------------------------------------------------------
INFO 14:48:44,453 HelpFormatter - The Genome Analysis Toolkit (GATK) v3.4-46-gbc02625, Compiled 2015/07/09 17:38:12
INFO 14:48:44,454 HelpFormatter - Copyright (c) 2010 The Broad Institute
INFO 14:48:44,454 HelpFormatter - For support and documentation go to http://www.broadinstitute.org/gatk
INFO 14:48:44,458 HelpFormatter - Program Args: -T HaplotypeCaller -R spinach_assembly-repeatdetect_PACBIO_V1.3_formated_60.fa -I .sam.list -drf DuplicateRead --alleles Unfiltered_Spinach_PacBio_Reseq_12_Geno_Assay_SNP.fixed.noblanks.vcf --genotyping_mode GENOTYPE_GIVEN_ALLELES --output_mode EMIT_ALL_SITES -o output_raw_unfiltered_spinach_snps_gbs.vcf
INFO 14:48:44,468 HelpFormatter - Executing as email@example.com on Linux 2.6.18-348.12.1.el5 amd64; Java HotSpot(TM) 64-Bit Server VM 1.8.0_05-b13.
INFO 14:48:44,469 HelpFormatter - Date/Time: 2015/07/31 14:48:44
INFO 14:48:44,469 HelpFormatter - ---------------------------------------------------------------------------------
INFO 14:48:44,470 HelpFormatter - ---------------------------------------------------------------------------------
INFO 14:48:45,102 GenomeAnalysisEngine - Strictness is SILENT
INFO 14:48:45,385 GenomeAnalysisEngine - Downsampling Settings: Method: BY_SAMPLE, Target Coverage: 500
INFO 14:48:45,394 SAMDataSource$SAMReaders - Initializing SAMRecords in serial
INFO 14:48:48,432 SAMDataSource$SAMReaders - Init 50 BAMs in last 3.04 s, 50 of 80 in 3.04 s / 0.05 m (16.46 tasks/s). 30 remaining with est. completion in 1.82 s / 0.03 m
INFO 14:48:50,052 SAMDataSource$SAMReaders - Done initializing BAM readers: total time 4.66
INFO 14:48:50,164 HCMappingQualityFilter - Filtering out reads with MAPQ < 20
INFO 14:48:54,742 RMDTrackBuilder - Writing Tribble index to disk for file /local/scratch/scratch/Amanda/Spinach_GBS/Unfiltered_Spinach_PacBio_Reseq_12_Geno_Assay_SNP.fixed.noblanks.vcf.idx
INFO 14:48:58,784 GenomeAnalysisEngine - Preparing for traversal over 80 BAM files
INFO 14:49:00,054 GATKRunReport - Uploaded run statistics report to AWS S3
I'm encountering a problem similar to what I experienced with a mismatch between reference and cosmic files. Specifically, I created .bam files using human_g1k_v3.fasta, which reference coordinates as 1...22,X,Y etc. When I try to run UnifiedGenotyper with this reference file, the .bam file I created, and the dbsnp_137.b37.vcf, I get an error on account of the fact that snp coordinates are listed as chr1...chr22,chrX,chrY etc. Other than writing a script to remove all occurrences of "chr" is there another way to get around this problem, i.e. a dbsnp reference file that has the desired coordinates without the "chr"?
I resolved the problem with reference/cosmic by finding a cosmic file with consistent notation, but can't find a similar fix for this one. I'd appreciate any suggestions.
Hi, I hope this is a quick question; but does using the 'include intervals' command line option '-L' only include the region specified?
For instance if I have an file that includes reads for chromosomes 1,2,6,X,and Y and I specifiy "-L 6", will the walker only process chromosome 6, or will it include the rest of my data as well?
Thank you for the clarification!
Hi everyone,
I'm using GATK Haplotype Caller and recently I read a document about optimization and GATK evoking GPU, IBM Power8, etc... Conclusions are that GATK could run faster than the actual implementation. In this document you suggest a C or C++ development in parallel of the actual Java implementation. And I wonder if you have made any progress so far and if you have planned a release? I'm also interested to know about the technology you're considering for GATK HC : C, C++, MPI, OpenMP, GPU...?
I have used the following commands using DepthOfCoverage tool with two different bed files:
java -jar GenomeAnalysisTK.jar -T DepthOfCoverage -R ucsc_hg19.fa -I WT_recalibrated.bam -L coverage_summary.bed -ct 1 -ct 10 -ct 20 -ct 30 -ct 50 -ct 100 -o WT_cov
The line count for the input and output:
$wc -l WT_cov.sample_interval_summary
4988 WT_cov.sample_interval_summary
$ wc -l coverage_summary.bed
10585 coverage_summary.bed
In the other case:
java -jar GenomeAnalysisTK.jar -T DepthOfCoverage -R ucsc_hg19.fa -I WT_recalibrated.bam -L exon.bed -ct 1 -ct 10 -ct 20 -ct 30 -ct 50 -ct 100 -o WT_exon
Line count for the input and output:
$ wc -l WT_exon.sample_interval_summary
5065 WT_exon.sample_interval_summary
$ wc -l exon.bed
5065 exon.bed
The input in both the cases is of the standard format as shown below:
chr1 6529578 6529755
chr1 6530273 6530442
chr1 6530543 6530721
chr1 6530773 6530980
chr1 6531028 6531730
chr1 6531768 6531914
chr1 6532563 6532713
chr1 6533023 6533273
Could anyone help to interpret the discrepancy between number of target regions in bed file and _interval_summary file in the above two cases?
Hi all, I tried to apply the following command to my raw vcf file to filter it with the command: java -Xmx30g -jar ../GATK/GenomeAnalysisTK.jar -R ../ref.fa -T VariantFiltration --filterExpression " QD < 20.0 || ReadPosRankSum < -8.0 || FS > 10.0 || QUAL < $MEANQUAL || MQ <30.0 || DP< 10.0 " --filterName LowQualFilter --missingValuesInExpressionsShouldEvaluateAsFailing --variant ../s1.raw.vcf --logging_level ERROR -o ../s1.makered.raw.vcf
grep -v "Filter" s1.makered.raw.vcf >s1.flt.vcf
After that, I checked the result file s1.flt.vcf and found the following makered "PASS" .Obviously, the command doesn't work as 'DP=8" should be makered "LowQualFiter".
Chr01 231575 . A G 241.78 PASS AC=2;AF=1.00;AN=2;DP=8;FS=0.000;MLEAC=2;MLEAF=1.00;MQ=29.00;MQ0=0;QD=30.22 GT:AD:DP:GQ:PL 1/1:0,8:8:24:270,24,0 Chr01 237476 . T C 238.78 PASS AC=2;AF=1.00;AN=2;DP=8;FS=0.000;MLEAC=2;MLEAF=1.00;MQ=29.00;MQ0=0;QD=29.85 GT:AD:DP:GQ:PL 1/1:0,8:8:24:267,24,0
There is no error reported.Any suggestion will be appreciated.
I'm trying to call variants on WGS data using the following command (on a high-performance cluster using 4 cores per job) :
java -Xmx6G -jar $CLASSPATH -T HaplotypeCaller --dbsnp GRCh37-lite.vcf -nct 4 -R GRCh37-lite.fa -I /user/data/gent/gvo000/gvo00027/vsc40035/StJude/001/001_D.bam -maxAltAlleles 10 --genotyping_mode DISCOVERY -stand_emit_conf 10 -stand_call_conf 30 -o 001_D.vcf
$CLASSPATH contains the location of the .jar file.
For 20 out of 70 samples the script ended without a problem, for the other 50 samples the same error messages was returned, given below. Can you tell what's going wrong?
Kind regards, Steve
INFO 07:28:05,514 ProgressMeter - 2:92269030 2.49e+08 3.1 h 44.0 s 11.0% 27.9 h 24.8 h INFO 07:29:05,515 ProgressMeter - 2:92309304 2.49e+08 3.1 h 44.0 s 11.0% 28.1 h 25.0 h INFO 07:30:05,516 ProgressMeter - 2:92320228 2.49e+08 3.1 h 44.0 s 11.0% 28.2 h 25.1 h INFO 07:30:06,052 GATKRunReport - Uploaded run statistics report to AWS S3
java.lang.NullPointerException at org.broadinstitute.sting.gatk.walkers.haplotypecaller.PairHMMLikelihoodCalculationEngine.computeDiploidHaplotypeLikelihoods(PairHMMLikelihoodCalculationEngine.java:443) at org.broadinstitute.sting.gatk.walkers.haplotypecaller.PairHMMLikelihoodCalculationEngine.computeDiploidHaplotypeLikelihoods(PairHMMLikelihoodCalculationEngine.java:417) at org.broadinstitute.sting.gatk.walkers.haplotypecaller.GenotypingEngine.calculateGLsForThisEvent(GenotypingEngine.java:385) at org.broadinstitute.sting.gatk.walkers.haplotypecaller.GenotypingEngine.assignGenotypeLikelihoods(GenotypingEngine.java:222) at org.broadinstitute.sting.gatk.walkers.haplotypecaller.HaplotypeCaller.map(HaplotypeCaller.java:880) at org.broadinstitute.sting.gatk.walkers.haplotypecaller.HaplotypeCaller.map(HaplotypeCaller.java:141) at org.broadinstitute.sting.gatk.traversals.TraverseActiveRegions$TraverseActiveRegionMap.apply(TraverseActiveRegions.java:708) at org.broadinstitute.sting.gatk.traversals.TraverseActiveRegions$TraverseActiveRegionMap.apply(TraverseActiveRegions.java:704) at org.broadinstitute.sting.utils.nanoScheduler.NanoScheduler$ReadMapReduceJob.run(NanoScheduler.java:471) at java.util.concurrent.Executors$RunnableAdapter.call(Executors.java:471) at java.util.concurrent.FutureTask.run(FutureTask.java:262) at java.util.concurrent.ThreadPoolExecutor.runWorker(ThreadPoolExecutor.java:1145) at java.util.concurrent.ThreadPoolExecutor$Worker.run(ThreadPoolExecutor.java:615) at java.lang.Thread.run(Thread.java:745)
I have used CombineVariants to combine variants from GATK and samtools as shown below:
java -jar GenomeAnalysisTK.jar -T CombineVariants -R ref.fa --variant:GatkSNP GATKsnp.vcf --variant:GatkINDEL GATKind.vcf --variant:SamSNP Samsnp.vcf --variant:SamINDEL Samind.vcf -o allvar.vcf -genotypeMergeOptions PRIORITIZE -priority GatkSNP,GatkINDEL,SamSNP,SamINDEL --filteredrecordsmergetype KEEP_UNCONDITIONAL
This merges all the variants. However, with the above command, i do get the variants present in both GATK and samtools emitted from samtools.
I would like to get all the variants such that:
could someone suggest any ideas or of there is something to be fixed in the command.
Dear GATK help team,
I have a cut chromosome file (cur 17) in which I have processed through sorting, alignment, adding headers, and even through the realignertargetcreator. Yet, when I would like to call my indels from the Indel realigned. I have received an error. ERROR MESSAGE: Badly formed genome loc: Parameters to GenomeLocParser are incorrect:The contig index 0 is bad, doesn't equal the contig index 17 of the contig from a string chr17
I have cut these chromosomes and processed the chr17 first since that is my region of interest, and did it since I thought it might save memory issues.
I am currently a newbie, have check the forum for help, yet only found one similar post with no solution. Please help-- stuck at this stage. My code for the index realigned is the following: java -jar /Users/yotsukurasohiya/build/softwares/GenomeAnalysisTK-3.2-2/GenomeAnalysisTK.jar -T IndelRealigner -R /Volumes/Pegasus/broadref/ucsc.hg19.fasta -I /Volumes/Pegasus/tmp/mardup.pregatk.bam -targetIntervals 2_target_intervals.list -known /Volumes/Pegasus/broadref/Mills_and_1000G_gold_standard.indels.hg19.vcf -known /Volumes/Pegasus/broadref/dbsnp_138.hg19.vcf -known /Volumes/Pegasus/broadref/1000G_phase1.snps.high_confidence.hg19.vcf -o 2_realigned_reads.bam
The heads that I have added are through picard softwares addorreplacereadgroups. SO=coordinate CREATE_INDEX=true SM=temp PL=Illumina PU=barcode LB=bar ID=id
Using GATK on command-line the CatVariants command fails.
Program version: GATK 3.1-1-g07a4bf8.
ERROR MESSAGE: Invalid command line: Malformed walker argument: Could not find walker with name: CatVariants
Code to invoke:
java -jar GenomeAnalysisTK-3.1-1/GenomeAnalysisTK.jar -T CatVariant -R file.fasta
Note that in the current documentation for CatVariants the example lists the name as 'org.broadinstitute.sting.tools.CatVariants' rather than just CatVariants. Trying the listed string fails with the same error.
Below is the command:
java -cp $CLASSPATH/GenomeAnalysisTK.jar org.broadinstitute.sting.tools.CatVariants \
-R GATK_ref/hg19.fasta \
-V ../GATK/VQSR/parallel_batch/raw.snps_indels-1.vcf \
-V ../GATK/VQSR/parallel_batch/raw.snps_indels-2.vcf \
-V ../GATK/VQSR/parallel_batch/raw.snps_indels-3.vcf \
-out ../GATK/VQSR/parallel_batch/combined_raw.snps_indels.vcf \
-log ../GATK/VQSR/parallel_batch/log/combined.log \
After this, the combined_raw.snps_indels.vcf file only contains the header from raw.snps_indels-1.vcf, what might be wrong?
I'm running the latest GATK nightly build to process human exome-seq data (has 12 samples). It seemed be faster than the older version until I run the HaplotypeCaller. The run summary shows it will take 14 days to finish. I am wondering if there's anything in my below command: How to make it faster without losing data in the output?
java -Xmx10g -Djava.io.tmpdir=/temp/GATK_temp
-jar $CLASSPATH/GenomeAnalysisTK.jar \
-T HaplotypeCaller \
-R ../GATK_ref/hg19.fasta \
-I ./compressedbam.list \
-L ../GATK_ref/hg19knownGene_UCSC_sorted.bed \
-log ../GATK/VQSR/log/HaplotypeCaller_20131018.log \
-o ../GATK/VQSR/raw.snps_indels.vcf
If I put my input files as a list in the file named "input.list", how do I set the output names? or do I just need to set the output folder and the output file names will be automatically named?
I started with BWA-MEM to do alignment, used Picard to process the .SAM files (converted to bam, reorder, addorreplacegroup, etc). The GATK version I'm using is version 2.5-2-gf57256b, I cannot run 2.6 because the server only has Java 6 and I cannot upgrade it to Java 7.
I got a huge stack of error message when I run this command line (RealignerTargetCrator):
java -Xmx2g -jar $CLASSPATH/GenomeAnalysisTK.jar \ -T RealignerTargetCreator \ -R /Volumes/files/Users/user1/GATK_ref/hg19.fasta \ -I sorted_Deduped_reorder_grp.bam \ -o ./GATK/forIndelRealigner.intervals>
The error messages are these (sorry, a lot): I don't know why GATK needs to connect to window server? what permission problem? I am using a Mac OS X built server (remote). Thank you
java.lang.InternalError: Can't connect to window server - not enough permissions.
at java.lang.ClassLoader$NativeLibrary.load(Native Method)
at java.lang.ClassLoader.loadLibrary0(ClassLoader.java:1827)
at java.lang.ClassLoader.loadLibrary(ClassLoader.java:1724)
at java.lang.Runtime.loadLibrary0(Runtime.java:823)
at java.lang.System.loadLibrary(System.java:1045)
at sun.security.action.LoadLibraryAction.run(LoadLibraryAction.java:50)
at java.security.AccessController.doPrivileged(Native Method)
at java.awt.Toolkit.loadLibraries(Toolkit.java:1605)
at java.awt.Toolkit.
Dear GATK Users,
Could anybody tell me how to identify the deletions from the bam file using GATK module?? Actually i used UnifiedGenotyper i am getting list like
gi|262 48155 . G A 80.77 . AC=1;AF=0.500;AN=2;BaseQRankSum=0.103;DP=10;Dels=0.00;FS=0.000;HaplotypeScore =0.0000;MLEAC=1;MLEAF=0.500;MQ=28.61;MQ0=0;MQRankSum=-1.453;QD=8.08;ReadPosRankSum=-0.336 GT:AD:DP:GQ:PL 0/1:5,5:10:99:109,0,146
Thanks Sridhar
Hi all: I find that among all the work flows of GATK http://www.broadinstitute.org/gatk/guide/topic?name=methods-and-workflows there are no workflows for RNA-seq analysis. I understand that GATK mainly focuses on variant calling, can anyone tell me how to use GATK for RNA-seq analysis?
thanks daniel
Hello Team,
I am attempting to run GATK's PhasebyTransmission command to phase a vcf file contains a father, mother, son trio generated from complete genomics mkvcf command.
After creating the ped file and running the command I generate the error: "MESSAGE: BUG: Attempted to get likelihoods as strings and neither the vector nor the string is set!". I am not exactly sure what this means.
When I check my file and the documentation I am able to see that the 'GL' field is contained in the file, but could this not be the case? I have attached a few lines from the vcf I am using.
Any help with resolving the this issue would be of great help.
Thank you
Hello, I`m new to GATK and Queue. I understand that we can write a QScript in Queue to generate separate GATK jobs and run them on a cluster of several nodes. Can we implement GATK or Queue on google hadoop?
I got this error message, when trying to use a file to specify at which positions to emit variants:
ERROR MESSAGE: Couldn't read file /lustre/scratch109/sanger/tc9/agv/wgs/pipeline/union4x.positions because The interval file /lustre/scratch109/sanger/tc9/agv/wgs/pipeline/union4x.positions does not have one of the supported extensions (.bed, .list, .picard, .interval_list, or .intervals). Please rename your file with the appropriate extension. Is there a GATK page describing those 5 file formats? Some of them are unknown to me; e.g. .list.
I asked my question here, but please ignore it: http://gatkforums.broadinstitute.org/discussion/2219/l-option
Thanks a lot.
Also, the error message does not mention support for vcf files, but the documentation does. Are vcf files supported?
hi all! I'm trying to complete my first GATK run, I'm doing the step in the "EXECUTION STEP" following section.
please tell me if the step execution are globally correct.
the step 4.1 isn't executed without -maxCycle 1500.
when try to execute 4.2 step I got the following error:
org.broadinstitute.sting.utils.exceptions.ReviewedStingException: Key 1036 is too large for dimension 2 (max is 1001)
at org.broadinstitute.sting.utils.collections.NestedIntegerArray.put(NestedIntegerArray.java:128)
at org.broadinstitute.sting.utils.recalibration.RecalibrationReport.parseAllCovariatesTable(RecalibrationReport.java:157)
at org.broadinstitute.sting.utils.recalibration.RecalibrationReport.
java -Xmx4g -Djava.io.tmpdir=/tmp -jar MarkDuplicates.jar INPUT=M9.bam OUTPUT=m9.marked.bam METRICS_FILE=metrics CREATE_INDEX=true VALIDATION_STRINGENCY=LENIENT
java -Xmx4g -jar GenomeAnalysisTK.jar -T RealignerTargetCreator -R ucsc.hg19.fasta -knowndbsnp_137.hg19.vcf -o m9.list -I m9.marked.bam
java -Xmx4g -Djava.io.tmpdir=/tmp -jar GenomeAnalysisTK.jar -I m9.marked.bam -R ucsc.hg19.fasta -T IndelRealigner -targetIntervals m9.list -known dbsnp_137.hg19.vcf -o m9.marked.realigned.bam
java -Xmx4g -jar GenomeAnalysisTK.jar -R ucsc.hg19.fasta -T ReduceReads -I m9.marked.realigned.bam -o m9.marked.realigned.reduce.bam
java -Djava.io.tmpdir=/tmp/flx-auswerter -Xmx4g -jar FixMateInformation.jar INPUT=m9.marked.realigned.reduce.bam OUTPUT=m9.marked.realigned.reduce.fixed.bam SO=coordinate VALIDATION_STRINGENCY=LENIENT CREATE_INDEX=true
java -Xmx4g -jar GenomeAnalysisTK.jar -l INFO -R ucsc.hg19.fasta -knownSites dbsnp_137.hg19.vcf -I m9.marked.realigned.reduce.fixed.bam -T BaseRecalibrator -maxCycle 1500 -cov ReadGroupCovariate -cov QualityScoreCovariate -o m9.recal_data.grp
4.2 ***********************************
java -Xmx4g -jar GenomeAnalysisTK.jar -T PrintReads -R ucsc.hg19.fasta -I m9.marked.realigned.reduce.fixed.bam -BQSR m9.recal_data.grp -o m9.marked.realigned.reduce.fixed.recal.bam
java -Xmx4g -jar GenomeAnalysisTK.jar -nct 4 --num_threads 4 -glm BOTH
-R ucsc.hg19.fasta
-T UnifiedGenotyper
--sample_ploidy 5
-I m9.marked.realigned.reduce.fixed.bam
-D dbsnp_137.hg19.vcf
-o m9.vcf
-stand_call_conf 20.0
-stand_emit_conf 20.0
-A DepthOfCoverage -A AlleleBalance
HI When I run Base recabrator with the following command:
java -Xmx4g -jar /usr/bin/GenomeAnalysisTK.jar -T BaseRecalibrator -I realignedBam.bam -R /data1/human_g1k_v37.fasta --knownSites /data1/snp132.vcf -o recalibration_report.grp
I get the following error :
INFO 07:15:53,380 HttpMethodDirector - I/O exception (javax.net.ssl.SSLException) caught when processing request: Unrecognized SSL message, plaintext connection?
INFO 07:15:53,380 HttpMethodDirector - Retrying request
INFO 07:15:53,386 HttpMethodDirector - I/O exception (javax.net.ssl.SSLException) caught when processing request: Unrecognized SSL message, plaintext connection?
INFO 07:15:53,387 HttpMethodDirector - Retrying request
INFO 07:15:53,393 HttpMethodDirector - I/O exception (javax.net.ssl.SSLException) caught when processing request: Unrecognized SSL message, plaintext connection?
INFO 07:15:53,393 HttpMethodDirector - Retrying request
INFO 07:15:53,398 HttpMethodDirector - I/O exception (javax.net.ssl.SSLException) caught when processing request: Unrecognized SSL message, plaintext connection?
INFO 07:15:53,398 HttpMethodDirector - Retrying request
INFO 07:15:53,405 HttpMethodDirector - I/O exception (javax.net.ssl.SSLException) caught when processing request: Unrecognized SSL message, plaintext connection?
INFO 07:15:53,405 HttpMethodDirector - Retrying request
##### ERROR A USER ERROR has occurred (version 2.0-34-g07bda93):
##### ERROR The invalid arguments or inputs must be corrected before the GATK can proceed
##### ERROR Please do not post this error to the GATK forum
##### ERROR See the documentation (rerun with -h) for this tool to view allowable command-line arguments.
##### ERROR Visit our website and forum for extensive documentation and answers to
##### ERROR commonly asked questions http://www.broadinstitute.org/gatk
##### ERROR MESSAGE: Invalid command line: No tribble type was provided on the command line and the type of the file could not be determined dynamically. Please add an explicit type tag :NAME listing the correct type from among the supported types:
##### ERROR Name FeatureType Documentation
##### ERROR BCF2 VariantContext http://www.broadinstitute.org/gatk/gatkdocs/org_broadinstitute_sting_utils_codecs_bcf2_BCF2Codec.html
##### ERROR BEAGLE BeagleFeature http://www.broadinstitute.org/gatk/gatkdocs/org_broadinstitute_sting_utils_codecs_beagle_BeagleCodec.html
##### ERROR BED BEDFeature http://www.broadinstitute.org/gatk/gatkdocs/org_broad_tribble_bed_BEDCodec.html
##### ERROR BEDTABLE TableFeature http://www.broadinstitute.org/gatk/gatkdocs/org_broadinstitute_sting_utils_codecs_table_BedTableCodec.html
##### ERROR EXAMPLEBINARY Feature http://www.broadinstitute.org/gatk/gatkdocs/org_broad_tribble_example_ExampleBinaryCodec.html
##### ERROR GELITEXT GeliTextFeature http://www.broadinstitute.org/gatk/gatkdocs/org_broad_tribble_gelitext_GeliTextCodec.html
##### ERROR OLDDBSNP OldDbSNPFeature http://www.broadinstitute.org/gatk/gatkdocs/org_broad_tribble_dbsnp_OldDbSNPCodec.html
##### ERROR RAWHAPMAP RawHapMapFeature http://www.broadinstitute.org/gatk/gatkdocs/org_broadinstitute_sting_utils_codecs_hapmap_RawHapMapCodec.html
##### ERROR REFSEQ RefSeqFeature http://www.broadinstitute.org/gatk/gatkdocs/org_broadinstitute_sting_utils_codecs_refseq_RefSeqCodec.html
##### ERROR SAMPILEUP SAMPileupFeature http://www.broadinstitute.org/gatk/gatkdocs/org_broadinstitute_sting_utils_codecs_sampileup_SAMPileupCodec.html
##### ERROR SAMREAD SAMReadFeature http://www.broadinstitute.org/gatk/gatkdocs/org_broadinstitute_sting_utils_codecs_samread_SAMReadCodec.html
##### ERROR TABLE TableFeature http://www.broadinstitute.org/gatk/gatkdocs/org_broadinstitute_sting_utils_codecs_table_TableCodec.html
##### ERROR VCF VariantContext http://www.broadinstitute.org/gatk/gatkdocs/org_broadinstitute_sting_utils_codecs_vcf_VCFCodec.html
##### ERROR VCF3 VariantContext http://www.broadinstitute.org/gatk/gatkdocs/org_broadinstitute_sting_utils_codecs_vcf_VCF3Codec.html
Hi I´ve a strange problem with the GATK. Everytime I try to run it my Console shows the following error Message.
30.10.12 15:10:06 [0x0-0xe20e2].com.apple.JarLauncher[1114] ##### ERROR ------------------------------------------------------------------------------------------
30.10.12 15:10:06 [0x0-0xe20e2].com.apple.JarLauncher[1114] ##### ERROR A USER ERROR has occurred (version 2.1-13-g0f021e6):
30.10.12 15:10:06 [0x0-0xe20e2].com.apple.JarLauncher[1114] ##### ERROR The invalid arguments or inputs must be corrected before the GATK can proceed
30.10.12 15:10:06 [0x0-0xe20e2].com.apple.JarLauncher[1114] ##### ERROR Please do not post this error to the GATK forum
30.10.12 15:10:06 [0x0-0xe20e2].com.apple.JarLauncher[1114] ##### ERROR
30.10.12 15:10:06 [0x0-0xe20e2].com.apple.JarLauncher[1114] ##### ERROR See the documentation (rerun with -h) for this tool to view allowable command-line arguments.
30.10.12 15:10:06 [0x0-0xe20e2].com.apple.JarLauncher[1114] ##### ERROR Visit our website and forum for extensive documentation and answers to
30.10.12 15:10:06 [0x0-0xe20e2].com.apple.JarLauncher[1114] ##### ERROR commonly asked questions http://www.broadinstitute.org/gatk
30.10.12 15:10:06 [0x0-0xe20e2].com.apple.JarLauncher[1114] ##### ERROR
30.10.12 15:10:06 [0x0-0xe20e2].com.apple.JarLauncher[1114] ##### ERROR MESSAGE: Argument with name '--analysis_type' (-T) is missing.
30.10.12 15:10:06 [0x0-0xe20e2].com.apple.JarLauncher[1114] ##### ERROR ------------------------------------------------------------------------------------------
Can you show me my mistakes please? With regards Oliver
During running of the depthOfCoverage tool, I get the error: /tmp/RsQHCt1W: No space left on device
I have tried changing the TMPDIR environment variable (and exporting) but eventually I get the same error. Is there a way to change the temporary directory that GATK uses?
I'm running GATK v2.1-8-g5efb575 on a Linux system.
Thanks, Rick