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Yingli Gets $324 Million From China Development Bank
Yingli Green Energy Holding Co., a Chinese solar-module maker, has a "framework agreement" for $324 million of China Development Bank funding for a so-called trade platform in Hong Kong used to import raw materials.
The state-run lender will provide 150 million yuan ($24 million) and $300 million, Yingli said in an e-mailed statement yesterday.
The platform will help Yingli reduce foreign-exchange and financing costs, it said. Yingli bought about 17 percent of raw materials through Hong Kong in 2011 and the proportion will probably surge to 40 percent this year, the company said.
China Development Bank has offered at least $47 billion in credit lines to help China's solar- and wind-energy companies since 2010. The support drew complaints from U.S. producers led by Solarworld AG that have sought duties on Chinese companies accused of using cheap funds from state lenders to sell solar panels on Western markets below the cost of production.
"It's just a framework agreement," Miao Qing, director of investor relations at Yingli, said in a telephone interview. "Yingli borrowed little from a $5.6 billion credit-line deal signed in 2010."

subset: commoncrawl


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For more than 35 years, NEDCO has been building opportunities in our community. By supporting financial stability and responsible homeownership, providing access to markets for local farmers and food producers, creating unique programs for micro-businesses and fueling the renewed interest in downtown Springfield, NEDCO is helping people build a future with promise.
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35 Comments | Add Yours
I'm celiac and I don't find this offensive. TV is tv, they push boundaries all the time. I'm much happier seeing something like this than watching a sex focused show like all the others. The only worry I have about this is the kids at schools starting this...
by XxStarlessNightxX on 5/22/2013 10:06:26 PM
I have a severe peanut allergy, where I'd most likely die if I eat it. My friends, as a joke, will wave their PB&J sandwich in my face and go "oooo". It's really scary for me and this is JUST like it. How rude.
by xxnycbeauty on 5/22/2013 9:38:42 PM
It's not that big of a deal, I mean it was just an episode. This is real life stuff and how is telling kids on disney to stop bullying and stuff, if they use that example of disney i can guaruntee you they will only get teased even more. This world is brutal
by hottie1250 on 5/22/2013 9:34:19 PM
As someone with a laundry list of allergies (and not just food allergies) I think that Disney needs to be more sensitive about stuff like this. Allergies can be deadly, and for some people just being near items containing their allergen can kill them. I'm lactose and soy intolerant, and the soy is worst. I accidentally eat one thing with soy in it and I'm sick for days. If I get stung by a bee, I could die, as very serious bee allergies run in my family (my mom and grandmother have both almost died from being stung.) I can't even use normal chapstick or lipstick or my face swells up, and my throat could close, all because I'm very, very, allergic to beeswax. I'm allergic to grass/plant oils/pollen, and just sitting in the grass with shorts on gives me welts and a rash. Poison ivy/oak could land me in the hospital. I'm even allergic to artificial frangrances, which means I can't even wear perfume without a rash and headache. And bullying, no matter how "funny" is always wrong.
by rockinlishy on 5/22/2013 9:30:21 PM
That's pretty mean of the show to make fun of kids with allergies like that. A good friend of mine can't eat anything with gluten.
by myfatsquirrel on 5/22/2013 9:30:03 PM
Are you kidding me? Where was the outrage when the writers of "Shake It Up" made a disgustingly insensitive joke about anorexia? I expected better of Girlslife. Instead of talking about that, you glorify their show and put them on the cover.In fact, I think all Disney writers should go to a 10 month course on reality and being sensitive to matters that affect youth today. Just sickening
by riot_grrrl on 5/22/2013 9:29:02 PM
That was kind of inconsiderate towards people with actual allergies, because they can be life threatening. But Disney, Dreamworks, and Nickolodeon have done worse things in the past. I was reading the Davinci Code, and apparently in the Lion King, if you pause it at a certain point, the fireflies spell s*x. And I only now realize how inapropriate Spongebob is, and it's hilarious behe cause all the 7 year olds that watch it have no idea how bad it is. Like the episode with the spanking train? What even?!
by prettypinkelephants on 5/22/2013 9:00:09 PM
Well I think that every is being entirely too sensitive. there are a lot more offensive things in this world than someone joking about your food allergy. everyone just needs to get over it. if I had that food allergy, I wouldn't care about the joke I would have laughed. so deleting the episode after all of there hard work because of one joke is stupid.
by cgirl109 on 5/22/2013 8:52:40 PM
While there is no need for Disney to be so boorish about allergies (saying that it affects many people and can come with life-threatening symptoms), it's not their responsibility nor has it ever been to educate any one. Jessie is a show that is made for entertainment, not education. I have to agree somewhat with HokeyLuver. In my opinion, I felt that Disney is always going overboard, so I never watch it. I don't feel that it is anyone's right to impose their opinion on Disney channel. If they don't like a joke that is being made or a message that is being displayed, they nor their children are being forced to watch it. They can simply change the channel and find something else to watch. I just don't understand why some people feel the need to smother everyone in their opinions.
by appleduck101 on 5/22/2013 8:03:25 PM
I agree with LittleUsagiTewi
by Chloe<3 on 5/22/2013 7:59:12 PM
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Date: 4 years ago
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A Lesson in True Style from African Diaspora Awards view
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464 Views 1 Reply Latest reply: Dec 8, 2012 2:05 PM by Limnos
Limnos Dec 8, 2012 2:05 PM
Re: how do i update my ipad from3.2.2 verson to the latest update and where in response to motorcycle1
iOS: How to update your iPhone, iPad, or iPod touch - http://support.apple.com/kb/HT4623 - If currently using pre-iOS5 you can only upgrade by connecting with iTunes, not with Software Update.
Back up your device first as updating it can erase content! See the suggestions in the second paragraph of King_Penguin's 12/2012 post https://discussions.apple.com/message/20520311


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text:
The expansin superfamily of plant proteins is made up of four families, designated α-expansin, β-expansin, expansin-like A and expansin-like B. α-Expansin and β-expansin proteins are known to have cell-wall loosening activity and to be involved in cell expansion and other developmental events during which cell-wall modification occurs. Proteins in these two families bind tightly to the cell wall and their activity is typically assayed by their stimulation of cell-wall extension and stress relaxation; no bona fide enzymatic activity has been detected for these proteins. α-Expansin proteins and some, but not all, β-expansin proteins are implicated as catalysts of 'acid growth', the enlargement of plant cells stimulated by low extracellular pH. A divergent group of β-expansin genes are expressed at high levels in the pollen of grasses but not of other plant groups. They probably function to loosen maternal cell walls during growth of the pollen tube towards the ovary. All expansins consist of two domains; domain 1 is homologous to the catalytic domain of proteins in the glycoside hydrolase family 45 (GH45); expansin domain 2 is homologous to group-2 grass pollen allergens, which are of unknown biological function. Experimental evidence suggests that expansins loosen cell walls via a nonenzymatic mechanism that induces slippage of cellulose microfibrils in the plant cell wall.
Gene organization and evolutionary history
Expansins are plant cell-wall loosening proteins involved in cell enlargement and in a variety of other developmental processes in which cell-wall modification occurs [1]. They are typically 250-275 amino acids long and are made up of two domains (domain 1 and domain 2) preceded by a signal peptide (Figure 1). On the basis of phylogenetic sequence analysis (Figure 2), four families of expansins are currently recognized in plants [2]. From the largest family to the smallest they are designated α-expansin (EXPA), β-expansin (EXPB), expansin-like A (EXLA) and expansin-like B (EXLB). α-Expansin and β-expansin proteins have been demonstrated experimentally to cause cell-wall loosening [3,4], whereas expansin-like A and expansin-like B proteins are known only from their gene sequences.
Figure 1. The domain structure of expansins and a comparison with that of distantly related single-domain plant proteins (G2A, p12 and barwin). The expansin signal peptide (SP) is 20-30 amino acids long, domain 1 is 120-135 amino acids, and domain 2 is 90-120 amino acids. Some barwin proteins have an additional chitin-binding domain after the signal peptide (not shown). The positions of the introns that are present in more than one expansin family are indicated by lettered triangles; homologous introns are present in p12 and barwin proteins. Intron letters are as in [7]. The position of intron B suggests that it could have participated in exon shuffling.
Figure 2. A phylogenetic tree of the expansin superfamily, including protein sequences from Arabidopsis thaliana (At), Oryza sativa (Os), Pinus species (pine) and Physcomitrella patens (moss). These sequences were selected to showcase expansin diversity. They were aligned with CLUSTALW (see Additional data file 1) and a neighbor-joining tree was constructed with MEGA 3. Bootstrap values above 60 are indicated next to the relevant node, and the four families are labeled at their roots. Clades, defined as all the descendants of the same ancestral gene in the last common ancestor of monocots and eudicots, are indicated by black bars to the right and given Roman numbers as in [7]. This tree does not correctly resolve clades EXPA-I and EXPA-II, possibly because of changes in amino-acid usage between Arabidopsis and rice expansins [7]. The numbers for pine sequences are from TIGR Pinus Gene Index [70]; GenBank accession numbers are shown for moss sequences. EXPA, α-expansin; EXPB, β-expansin; EXLA, expansin-like A; EXLB, expansin-like B.
It has not been established when expansins first appeared in evolution, but the α-expansin and β-expansin families already existed by the time the vascular plants and mosses diverged (Figure 2) [5,6]. So far, the expansin-like A and expansin-like B families can be traced back only to the last ancestor of angiosperms and gymnosperms (Figure 2). More recently the expansin families have continued to grow and diversify in different plant lineages. Table 1 shows the number of genes for each family found in the available angiosperm genomes, as well as the numbers of genes estimated for the last common ancestor of eudicots (including Arabidopsis) and monocots (including rice). On the basis of this reconstruction, we have recently proposed a subdivision of the four expansin families of angiosperms into 17 clades (Figure 2) [7]. As shown in Table 1, the number of genes has doubled in the Arabidopsis lineage and more than tripled in rice since these two species diverged, approximately 150 million years ago. The main reason for this difference is the larger number of tandem duplications present in the rice genome (Figure 3). The growth of the β-expansin family in grasses is particularly impressive, with 18 genes in rice compared with 6 in Arabidopsis.
Table 1. Sizes of the four expansin families in different plants
Figure 3. Genomic locations of expansin genes. (a) Arabidopsis; (b) rice. Genes in tandem are indicated by triangles and chromosome numbers are shown with Roman numerals. EXPA, α-expansin; EXPB, β-expansin; EXLA, expansin-like A; EXLB, expansin-like B.
Curiously, grasses (but only grasses) also have an additional group of secreted proteins homologous only to expansin domain 2; these are known in the immunological literature as grass group-2 pollen allergens (G2As). They seem to have evolved from a truncated copy of a β-expansin gene and they share about 35-45% protein identity with their closest β-expansin relatives; their native biological function is uncertain. Although G2As evolved from a β-expansin ancestor, because of the loss of domain 1 they are considered a separate family and not part of the expansin superfamily.
Two other families of plant proteins show distant homology to expansin domain 1, but as they lack domain 2 they are not considered part of the superfamily. The closest (approximately 25-35% identity) has been variously called p12 and plant natriuretic peptide (PNP). These proteins become abundant in the xylem of blighted citrus trees [8], and they have been ascribed a signaling function [9,10]. No wall-loosening activity has been found in extracts containing p12 (D.J.C. and T. Ceccardi, unpublished observations). More distantly related (about 20-30% identity) is the barwin-like domain that defines the PR4 family of antifungal proteins [11]. Both these protein families were already present in the last ancestor of mosses and vascular plants.
Turning to non-plant organisms, various proteins with distant homology to full-length expansins or exclusively to domain 1 are found from bacteria to nematodes and mollusks [12-15]. Many of these are probably involved in the digestion of plant cell-wall material. A family of expansin-like proteins has been found in the slime mold Dictyostelium discoideum, where they could help to maintain the fluidity of the cellulosic cell walls in the stalk structure [16]. Recent nomenclature rules [2] recommend that only proteins with homology to both expansin domains should be designated expansins. The polyphyletic group of non-plant expansins, such as those in Dictyostelium, can be referred to as expansin-like family X (EXLX). The relationship of the various groups of expansin-like X proteins with the plant expansins is unclear at the moment. Their divergence could predate the origin of land plants, or they could have been acquired later through horizontal transfer of a gene from one of the plant expansin families. The same applies to proteins with homology only to domain 1, both in plants and other organisms, in that it is possible that some of them originally evolved from an expansin protein with both domains.
Characteristic structural features
Expansin proteins from different families share only 20-40% identity with each other. The degree of conservation is highest in domain 1, as shown in Figure 4. Expansin domain 1 has a distant homology to glycoside hydrolase family 45 (GH45) proteins [17], most of which are fungal β-1,4-D-endoglucanases. Proteins from this family have been crystallized and their mechanism of action determined [18]: they form a six-stranded β-barrel with a groove for substrate binding (Figure 5a). Barwin also has a similar β-barrel structure [19]. On the basis of hydrophobic cluster analysis, we expect this structural motif also to be present in expansins (Figure 6). Furthermore, expansin domain 1 shares with GH45 a number of conserved cysteines that form disulfide bridges in the fungal enzymes. It is interesting that several residues that make up the catalytic site of GH45 endoglucanases are also conserved in expansin (see Figures 4, 5). Despite the presence of these conserved GH45 motifs, no hydrolytic activity has been detected for either α-expansin or β-expansin proteins.
Figure 4. Sequence conservation in the expansin superfamily. Sequence logos for the four expansin families were generated with WebLogo [71] and manually aligned. The signal peptide and the poorly conserved amino terminus of the mature proteins have been removed from the alignment; because some expansins have exceptionally large signal peptides and amino-terminal extensions the alignment starts around position 60. In these sequence logos the height of the stack of amino-acid symbols at each position indicates the degree of sequence conservation, and the height of each letter within the stack indicates the frequency of the corresponding amino acid. Residues conserved between families are shaded, and the boundary between the two domains is indicated by arrows. Key residues that are part of the catalytic site of GH45 proteins and that are conserved in domain 1 of some expansin families are shown in circles above the logos. EXPA, α-expansin; EXPB, β-expansin; EXLA, expansin-like A; EXLB, expansin-like B.
Figure 5. Structure of proteins homologous to expansin domains. (a) Expansin domain 1 (the catalytic domain of a GH45 endoglucanase from Humicola insolens; Protein Data Bank (PDB) code 2ENG). (b) Expansin domain 2 (a G2A protein from Phleum pratense; PDB 1WHO). In (a), the domain forms a β barrel; amino-acid residues that are conserved in expansins are indicated in the single-letter amino-acid code.
Figure 6. Aligned hydrophobic cluster analysis (HCA) plots of the catalytic domains of two GH45 proteins and domain 1 of an α-expansin protein, Arabidopsis EXPA15, with additional annotation based on the crystal structure of Humicola GH45. The GH45 sequences are from Humicola insolens (GenBank accession number P43316) and Trichoderma reesei (AAQ21385). HCA plots were constructed with DrawHCA [72]. In these plots, the amino-acid sequence of each protein is written out in duplicate in a helical representation that puts together amino-acid residues that would be next to each other in an α helix. The six β sheets that form a barrel in the GH45 from Humicola (see Figure 5a) are indicated by boxes above the plot. Cytosine residues involved in intramolecular bridges and conserved in expansins and GH45 proteins are shown by blue dots connected by blue lines, also above the plot. Selected conserved motifs are highlighted in pink and the differences in their relative positions between proteins are indicated by black lines between the plots. The interpretation of HCA plots is summarized in [73]. HCA uses the standard one-letter amino acid abbreviations except for four amino acids, as shown in the key. Hydrophobic residues are outlined. Clusters of hydrophobic residues are usually associated with regular secondary structures (α helices or β sheets). Zigzagging vertical lines of hydrophobic residues indicate alternating hydrophobic and non-hydrophobic residues, typical of exposed β sheets (for example, β2, β3, β5 and β6). Continuous hydrophobic clusters are more common in internal β sheets (for example, β4). Conservation of clusters and sequence motifs suggests that the core β-barrel structure with stabilizing cysteine bridges is conserved in the three proteins and that the differences are mostly in the size of the intervening loops. In Humicola GH45, the loops between β1 and β2 and between β5 and β6 have expanded considerably, while the other loops appear reduced in comparison with Trichoderma GH45. The latter appears more similar to expansin domain 1, which has an even more compact structure.
α-Expansin proteins can be distinguished from other expansins by the presence of a large insertion and a nearby deletion in domain 1; these are at either side of a conserved motif that is part of the conserved GH45 active site (HFD in the single-letter amino-acid code; Figure 4). Expansin-like A and expansin-like B proteins lack the HFD motif, which suggests that their action may differ from that of other expansins. Furthermore, expansin-like A proteins have a unique conserved motif (CDRC) at the amino terminus of domain 1, and their domain 2 has an extension of approximately 17 amino acids that is not found in other expansin families (Figure 4). The functional implications of these differences among families are currently unknown.
No proteins homologous to expansin domain 2 have yet been identified except for the G2A family. The structure of a G2A protein consists of two stacked β sheets with an immunoglobulin-like fold (Figure 5b) [20]. On the basis of this structure, some highly conserved aromatic residues present in expansin domain 2 have been hypothesized to form a binding strip for cell-wall polysaccharides [1,21], but this speculative idea has yet to be tested experimentally.
Localization and function
Expansins were first identified as wall-loosening proteins in studies of 'acid-induced growth' [3,22-24]. It was known for years that low extracellular pH (< 5.5) causes cell-wall loosening in land plants as well as in a subset of green algae that have walls of similar structure [25]. The process is mediated in large part by wall-bound expansins with an acidic pH optimum [3]. Wall pH is normally determined by the activity of the plasma membrane H+ ATPase, which pumps protons to the cell wall; the pH of the wall is typically about 5.5 but may go below 4.5 in some circumstances [25,26]. Acid-induced growth and expansin action are implicated in the growth responses of plants to hormones and to external stimuli such as light, drought, salt stress and submergence (anoxia) and in morphogenetic processes such as root-hair formation [27-31].
Expansin activity is usually assayed as the ability of a protein sample to induce extension of isolated cell walls (Figure 7). It may also be measured in stress-relaxation assays, in which the decay in wall stress is measured after the wall is rapidly extended and then held to a constant dimension [22]. Plant cell walls extend or relax by a process of molecular 'creep', in which the cellulose microfibrils and associated matrix polysaccharides separate from one another [32]. The energy needed to overcome the viscous resistance and entanglement of wall polymers comes from cell-wall stress, which in living plants arises from the turgor pressure within cells. Such molecular creep occurs only when the cell wall is loosened by expansins or by other factors (Figure 8); otherwise, the cellulose microfibrils are firmly held in place by matrix polysaccharides [27]. Artificial cell walls made of bacterial cellulose and xyloglucan have also been used as materials to investigate expansin action [33].
Figure 7. A common method for measuring the cell-wall extension activity of expansins. (a) Cell-wall specimens are excised from the growing region of a young seedling that has been grown in the dark (etiolated). The specimens are frozen and thawed in order to destroy the cells but leave the cell walls intact (the cuticle is abraded to facilitate penetration of proteins). The specimens are heat-treated to inactivate endogenous expansins and then clamped under constant tension in an extensometer. The extensometer measures the change in length of the sample, with or without the addition of exogenous expansins. Walls may be collected in parallel from other seedlings and extracted to obtain fractions with expansin activity, assayed as an increase in cell-wall length. (b) Photograph of a typical cell wall sample, placed on an index finger for scale, prior to clamping in the extensometer. (c) Time course for irreversible wall extension (creep) of heat-treated walls with and without the addition of expansin.
Figure 8. A simplified model of the plant cell wall and its loosening by expansins. The cell wall consists of a scaffold of cellulose microfibrils (shaded areas) to which are bound various glycans such as xyloglucan or xylan (thin strands); together these polysaccharides form a strong, flexible, load-bearing network based on hydrogen bonds (indicated by rows of short lines). Extension of the cell wall entails movement and separation of the cellulose microfibrils by a process of molecular creep. α-Expansins (EXPA) may promote such movement by inducing local dissociation and slippage of xyloglucans on the surface of the cellulose, whereas β-expansins (EXPB) work on a different glycan, perhaps xylan, for similar effect. Expansin-like A (EXLA) and expansin-like B (EXLB) proteins are predicted to be secreted to the cell wall, but their activity has not yet been established.
Expansin activity is most often associated with cell-wall loosening in growing cells [34]; this connection has been confirmed and extended by experiments in which expansin gene expression is manipulated in transgenic plants [35-38]. In most cases, silencing of expansin genes leads to inhibition of growth, whereas excessive ectopic expression leads to faster or abnormal growth. Localized expression of expansins is associated with the meristems and growth zones of the root and stem, as well as the formation of leaf primordia on shoot apical meristems [39] and the outgrowth of the epidermal cell walls during root-hair formation [40]. Additionally, expansins are implicated in other developmental processes during which wall loosening occurs, such as fruit softening [41-46], xylem formation [47], abscission (leaf shedding) [48], seed germination [49], penetration of pollen tubes through the stigma and style [4,50], formation of mycorrhizal associations with symbiotic fungi in root tissues [51], development of nitrogen-fixing nodules in legumes [52], development of parasitic plants [53,54], and rehydration of 'resurrection' plants, which curl up tightly when dry and expand when wet [55]. Some plants that are adapted to an aquatic environment respond to submergence with a pronounced elongation. This depends on wall acidification [56] and is correlated with activation of expansin gene expression [57-59].
In cell-fractionation studies, expansins are found bound to the cell wall, as expected from their activity [23,60,61]. With immunolocalization by light and electron microscopy, expansin proteins were localized to the cell wall [51,61,62], where they were found to be distributed throughout the thickness of the walls rather than concentrated in specific strata. There is at least one report that expansin mRNA can be found specifically at the polar ends of developing xylem cells [63]; transcript localization may be a means for ensuring that protein production and secretion is directed to the ends of these cells. It is not clear whether this mRNA targeting is unique to expansins in xylem or whether it is a more general phenomenon. Finally, grass pollen produces and secretes specialized β-expansin proteins in copious amounts (they are known as grass pollen group-1 allergens) [4,64], but this is an unusual situation: expansins in other tissues have been found only at low concentrations.
Mechanism and regulation
All the α-expansin proteins that have been characterized so far have a pH optimum for cell-wall extension of about 4 [3,23,60]. This situation permits the cell to regulate α-expansin activity rapidly by modulating wall pH. The pH optimum of only one class of β-expansin proteins has been characterized, namely the group-1 grass pollen allergens (such as EXPB1 from maize), and it has a broad pH optimum centered at about 5.5 [65]. These pollen proteins are probably not involved in acid growth but rather in the wall loosening that is associated with invasion of maternal tissues by pollen tubes. It is expected that β-expansin proteins in somatic tissues have a pH dependence more similar to that of α-expansins, but so far β-expansin proteins in an active state have not been extracted from somatic tissues, so this expectation remains to be tested experimentally. Also, both α-expansin and β-expansin proteins are activated by reducing agents [3,4,65]; this could be biologically significant, as the cell-wall redox potential can be modulated by electron transport across the plasma membrane.
Expansins do not have hydrolytic activity or any of the other enzymatic activities yet assayed [64,66,67]. A report that they are proteases was later refuted [64]. Expansins also act very quickly - they induce rapid extension within seconds of addition to wall specimens, but they do not affect the plasticity or elasticity of the cell wall [68]. In contrast, cell-wall creep caused by an endoglucanase has a long lag time and is accompanied by large increases in wall plasticity and elasticity, indicative of major structural changes in the cell wall (cutting of cross-links) [68]. Thus, expansin's effects on cell walls are distinct from those expected of hydrolytic enzymes.
A nonenzymatic mechanism has been proposed for expansin action, in which expansin disrupts noncovalent bonds that tether matrix polysaccharides to the surface of cellulose microfibrils or to each other [1,66,69]. In this model, the expansin is thought to act like a zipper that enables microfibrils to move apart from each other by ungluing the chains that stick them together. This idea is also supported by experiments in which an expansin is applied to artificial composites made of bacterial cellulose and xyloglucan [33]. Whatever their biochemical mechanism of action, expansins act in catalytic amounts to stimulate wall polymer creep without causing major covalent alterations of the cell wall [66].
In the published literature on expansins, gene expression has drawn the greatest amount of attention, but given the large size of the superfamily, the expression and presumptive role of many expansin genes remains unexplored. Although expression of specific expansin genes has been shown to be induced by hormones, by submergence, by drought stress, or by other stimuli, the signaling pathway has not been worked out in detail in even a single case. Major biochemical questions also remain regarding the specific wall polysaccharides on which expansins act, the differences between the action of α-expansins and β-expansins, and the molecular mechanisms underlying wall loosening. Answering these questions will require a much deeper understanding of cell-wall structure and in particular of how the cell wall is able to expand in a controlled fashion. Finally, it remains to be established whether expansin-like A and expansin-like B proteins have cell-wall loosening activity or not.
Additional data files
An alignment of the sequences used to make the phylogenetic tree in Figure 2 is available as Additional data file 1.
Additional data file 1. An alignment of the sequences used to make the phylogenetic tree in Figure 2
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This work has been supported by grants to D.J.C. from the US National Science Foundation, the Department of Energy, and the National Institutes of Health.
Cosgrove DJ: Loosening of plant cell walls by expansins.
Nature 2000, 407:321-326.
A review of cell-wall structure and the action of expansins.PubMed Abstract | Publisher Full Text
Kende H, Bradford K, Brummell D, Cho HT, Cosgrove DJ, Fleming A, Gehring C, Lee Y, Queen-Mason S, Rose J, Voesenek LA: Nomenclature for members of the expansin superfamily of genes and proteins.
Plant Mol Biol 2004, 55:311-314.
This article by the expansin research community defines expansins and recommends naming conventions.PubMed Abstract | Publisher Full Text
McQueen-Mason S, Durachko DM, Cosgrove DJ: Two endogenous proteins that induce cell wall expansion in plants.
Plant Cell 1992, 4:1425-1433.
The purification of two related proteins that cause pH-dependent wall extension, later named expansins.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Cosgrove DJ, Bedinger P, Durachko DM: Group I allergens of grass pollen as cell wall-loosening agents.
Proc Natl Acad Sci USA 1997, 94:6559-6564.
Shows that group-1 grass pollen allergens have expansin activity and defines the second family of expansins (β-expansins)PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Li Y, Darley CP, Ongaro V, Fleming A, Schipper O, Baldauf SL, McQueen-Mason SJ: Plant expansins are a complex multigene family with an ancient evolutionary origin.
Plant Physiol 2002, 128:854-864.
Phylogenetic analysis of the expansin superfamily in Arabidopsis and identification of more distantly related sequences; also proposes a nomenclature system superseded by [2].PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Schipper O, Schaefer D, Reski R, Fleming A: Expansins in the bryophyte Physcomitrella patens.
Plant Mol Biol 2002, 50:789-802.
Identification of expansin family members in a moss and analysis of gene expression.PubMed Abstract | Publisher Full Text
Sampedro J, Lee Y, Carey RE, dePamphilis CW, Cosgrove DJ: Use of genomic history to improve phylogeny and understanding of births and deaths in a gene family.
Plant J 2005, 44:409-419.
Traces the evolution of the expansin superfamily in Arabidopsis and rice since their last common ancestor.PubMed Abstract | Publisher Full Text
Ceccardi TL, Barthe GA, Derrick KS: A novel protein associated with citrus blight has sequence similarities to expansin.
Plant Mol Biol 1998, 38:775-783.
Identification of a 12 kDa protein (p12) in xylem of blighted citrus trees that is distantly related to expansin domain 1.PubMed Abstract | Publisher Full Text
Rafudeen S, Gxaba G, Makgoke G, Bradley G, Pironcheva G, Raitt L, Irving H, Gehring C: A role for plant natriuretic peptide immuno-analogues in NaCl- and drought-stress responses.
Physiol Plant 2003, 119:554-562.
Implicates a 12 kDa protein, with distant sequence similarity to expansin domain 1, in plant water stress responses.Publisher Full Text
Gehring CA, Irving HR: Natriuretic peptides - a class of heterologous molecules in plants.
Int J Biochem Cell Biol 2003, 35:1318-1322.
Reviews the properties of PNPs, which are homologous to expansin domain 1.PubMed Abstract | Publisher Full Text
Friedrich L, Moyer M, Ward E, Ryals J: Pathogenesis-related protein 4 is structurally homologous to the carboxy-terminal domains of hevein, Win-1 and Win-2.
Mol Gen Genet 1991, 230:113-119.
Characterizes the PR-4 family, whose barwin-like domain is distantly related to expansin domain 1.PubMed Abstract | Publisher Full Text
Laine M, Haapalainen M, Wahlroos T, Kankare K, Nissinen R, Kassuwi S, Metzler MC: The cellulase encoded by the native plasmid of Clavibacter michiganensis subsp. sepedonicus plays a role in virulence and contains an expansin-like domain.
Physiol Mol Plant Pathol 2001, 57:221-233.
A Clavibacter virulence factor contains a domain distantly related to expansin domain 1.Publisher Full Text
Xu B, Janson JC, Sellos D: Cloning and sequencing of a molluscan endo-beta-1,4-glucanase gene from the blue mussel, Mytilus edulis.
Eur J Biochem 2001, 268:3718-3727.
Cloning and sequence analysis of the first family-45 endoglucanase from a mollusk.PubMed Abstract | Publisher Full Text
Kudla U, Qin L, Milac A, Kielak A, Maissen C, Overmars H, Popeijus H, Roze E, Petrescu A, Smant G, et al.: Origin, distribution and 3D-modeling of Gr-EXPB1, an expansin from the potato cyst nematode Globodera rostochiensis.
FEBS Lett 2005, 579:2451-2457.
Phylogenetic analysis and molecular modeling of a nematode protein with homology to expansin domain 1.PubMed Abstract | Publisher Full Text
Saloheimo M, Paloheimo M, Hakola S, Pere J, Swanson B, Nyyssonen E, Bhatia A, Ward M, Penttila M: Swollenin, a Trichoderma reesei protein with sequence similarity to the plant expansins, exhibits disruption activity on cellulosic materials.
Eur J Biochem 2002, 269:4202-4211.
Identification of a fungal protein with distant sequence similarity to expansin and which disrupts cotton fibers with hydrolytic action.PubMed Abstract | Publisher Full Text
Darley CP, Li Y, Schaap P, McQueen-Mason SJ: Expression of a family of expansin-like proteins during the development of Dictyostelium discoideum.
FEBS Lett 2003, 546:416-418.
Identifies a small group of Dictyostelium genes related to expansins and characterizes their expression during development.PubMed Abstract | Publisher Full Text
Carbohydrate-Active enZYmes [http://afmb.cnrs-mrs.fr/CAZY/] webcite
A database that describes the families of structurally related catalytic enzymes that degrade, modify, or create glycosidic bonds.
Davies GJ, Tolley SP, Henrissat B, Hjort C, Schulein M: Structures of oligosaccharide-bound forms of the endoglucanase V from Humicola insolens at 1.9 Å resolution.
Biochemistry 1995, 34:16210-16220.
A structural analysis of the Humicola GH45 enzyme complexed with cello-oligosaccharide identifies key catalytic residues as well as a large conformational change in the enzyme upon substrate binding.PubMed Abstract | Publisher Full Text
Ludvigsen S, Poulsen FM: Three-dimensional structure in solution of barwin, a protein from barley seed.
Biochemistry 1992, 31:8783-8789.
The barwin structure includes a β barrel somewhat resembling the catalytic domain of GH45 proteins.PubMed Abstract | Publisher Full Text
Fedorov AA, Ball T, Valenta R, Almo SC: X-ray crystal structures of birch pollen profilin and Phl p 2.
Int Arch Allergy Immunol 1997, 113:109-113.
Structural analysis of the group-2 grass pollen allergen, Phl p 2, which is homologous to expansin domain 2.PubMed Abstract
Barre A, Rouge P: Homology modeling of the cellulose-binding domain of a pollen allergen from rye grass: structural basis for the cellulose recognition and associated allergenic properties.
Biochem Biophys Res Commun 2002, 296:1346-1351.
A homology model of domain 2 from EXPB with identification of a groove and extended strip of aromatic and polar residues that might function in carbohydrate binding.PubMed Abstract | Publisher Full Text
Cosgrove DJ: Characterization of long-term extension of isolated cell walls from growing cucumber hypocotyls.
Planta 1989, 177:121-130.
An analysis of acid growth of isolated cell walls, hypothesizing a wall-loosening enzyme with unusual properties, later purified and named expansin.PubMed Abstract | Publisher Full Text
Li Z-C, Durachko DM, Cosgrove DJ: An oat coleoptile wall protein that induces wall extension in vitro and that is antigenically related to a similar protein from cucumber hypocotyls.
Planta 1993, 191:349-356.
The first use of the name expansin; this article identifies a wall-loosening protein from oat seedlings with many similarities to cucumber expansins.Publisher Full Text
Shcherban TY, Shi J, Durachko DM, Guiltinan MJ, McQueen-Mason SJ, Shieh M, Cosgrove DJ: Molecular cloning and sequence analysis of expansins - a highly conserved, multigene family of proteins that mediate cell wall extension in plants.
Proc Natl Acad Sci USA 1995, 92:9245-9249.
The cloning of α-expansin shows that it belongs to a multigene family and lacks sequence similarity to any enzymes known at the time of publication.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Rayle DL, Cleland RE: The acid growth theory of auxin-induced cell elongation is alive and well.
Plant Physiol 1992, 99:1271-1274.
Reviews the evidence that cell-wall acidification is part of the mechanism of auxin-induced cell elongation.PubMed Abstract | PubMed Central Full Text
Bibikova TN, Jacob T, Dahse I, Gilroy S: Localized changes in apoplastic and cytoplasmic pH are associated with root hair development in Arabidopsis thaliana.
Development 1998, 125:2925-2934.
Shows that outgrowth of the outer epidermal cell wall during root-hair initiation starts with local wall acidification (to pH 4.5).PubMed Abstract | Publisher Full Text
Cosgrove DJ: Growth of the plant cell wall.
Nat Rev Mol Cell Biol 2005, 6:850-861.
This review summarizes wall structure and recent progress in understanding the biosynthesis and expansion of the plant cell wall.PubMed Abstract | Publisher Full Text
Bogoslavsky L, Neumann PM: Rapid regulation by acid pH of cell wall adjustment and leaf growth in maize plants responding to reversal of water stress.
Plant Physiol 1998, 118:701-709.
Provides evidence that water availability affects leaf-cell elongation by changes in cell-wall acidification and consequent changes in wall extensibility.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Sabirzhanova IB, Sabirzhanov BE, Chemeris AV, Veselov DS, Kudoyarova GR: Fast changes in expression of expansin gene and leaf extensibility in osmotically stressed maize plants.
Plant Physiol Biochem 2005, 43:419-422.
Maize seedlings respond to osmotic stress by an increase in wall extensibility that is correlated with an increase in EXPA transcript levels.PubMed Abstract | Publisher Full Text
Link BM, Cosgrove DJ: Acid-growth response and alpha-expansins in suspension cultures of bright yellow 2 tobacco.
Plant Physiol 1998, 118:907-916.
Shows that cells in suspension culture elongate faster in response to fusicoccin (which induces wall acidification) and express expansins at the mRNA and protein level. Cells also grow faster upon treatment with exogenous expansin.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Downes BP, Steinbaker CR, Crowell DN: Expression and processing of a hormonally regulated beta-expansin from soybean.
Plant Physiol 2001, 126:244-252.
Auxin and cytokinin synergistically enhance the accumulation of EXPB protein (CIM1) in soybean cell cultures. Evidence for stepwise proteolysis of the extracellular protein is also reported.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Marga F, Grandbois M, Cosgrove DJ, Baskin TI: Cell wall extension results in the coordinate separation of parallel microfibrils: evidence from scanning electron microscopy and atomic force microscopy.
Plant J 2005, 43:181-190.
Shows that cell-wall extension does not entail passive reorientation of cellulose microfibrils, implying a specific loosening mechanism that results in lateral separation of microfibrils.PubMed Abstract | Publisher Full Text
Whitney SE, Gidley MJ, McQueen-Mason SJ: Probing expansin action using cellulose/hemicellulose composites.
Plant J 2000, 22:327-334.
EXPA induces rapid, transient extension of artificial cellulosic composites (derived from Acetobacter pellicles) made with xyloglucan, but not those made with glucomannan or galactomannan.PubMed Abstract | Publisher Full Text
Lee Y, Choi D, Kende H: Expansins: ever-expanding numbers and functions.
Curr Opin Plant Biol 2001, 4:527-532.
A review of expansin gene expression and gene structure and their implications for expansin function and evolution.PubMed Abstract | Publisher Full Text
Cho HT, Cosgrove DJ: Altered expression of expansin modulates leaf growth and pedicel abscission in Arabidopsis thaliana.
Proc Natl Acad Sci USA 2000, 97:9783-9788.
Transgenic experiments to increase or reduce expansin gene expression support the role of expansin in cell growth and in abscission.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Choi DS, Lee Y, Cho HT, Kende H: Regulation of expansin gene expression affects growth and development in transgenic rice plants.
Plant Cell 2003, 15:1386-1398.
Plants expressing an antisense EXPA RNA were shorter than controls, whereas plants overexpressing the EXPA gene had more leaves but a mixed phenotype, some taller and some shorter than controls.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Pien S, Wyrzykowska J, McQueen-Mason S, Smart C, Fleming A: Local expression of expansin induces the entire process of leaf development and modifies leaf shape.
Proc Natl Acad Sci USA 2001, 98:11812-11817.
The authors used transient local micro-induction of EXPA genes on the shoot apical meristem and the flanks of leaf primordia to demonstrate that EXPA overexpression can markedly stimulate plant cell growth.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Lee DK, Ahn JH, Song SK, Choi YD, Lee JS: Expression of an expansin gene is correlated with root elongation in soybean.
Plant Physiol 2003, 131:985-997.
This study shows that GmEXPA1 expression is maximal in the elongation zone of the soybean root, and ectopic expression of this gene in tobacco roots leads to faster root growth.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Reinhardt D, Wittwer F, Mandel T, Kuhlemeier C: Localized upregulation of a new expansin gene predicts the site of leaf formation in the tomato meristem.
Plant Cell 1998, 10:1427-1437.
Shows that localized expression of an EXPA gene on the flanks of the shoot apical meristem precedes emergence of the leaf primordium.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Cho HT, Cosgrove DJ: Regulation of root hair initiation and expansin gene expression in Arabidopsis.
Plant Cell 2002, 14:3237-3253.
Two expansin genes (AtEXPA7 and AtEXP18) are turned on specifically at the place and time of root-hair formation.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Brummell DA, Harpster MH, Civello PM, Palys JM, Bennett AB, Dunsmuir P: Modification of expansin protein abundance in tomato fruit alters softening and cell wall polymer metabolism during ripening.
Plant Cell 1999, 11:2203-2216.
Transgenic tomato fruits with higher levels of LeEXPA1 gene expression were much softer than controls, whereas fruits with reduced LeEXPA1 expression were firmer.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Rose JK, Lee HH, Bennett AB: Expression of a divergent expansin gene is fruit-specific and ripening-regulated.
Proc Natl Acad Sci USA 1997, 94:5955-5960.
Identifies a tomato EXPA gene (LeEXPA1) that is expressed during fruit ripening and proposes a role for expansins in cell-wall disassembly in nongrowing tissues.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Kalamaki MS, Powell AL, Struijs K, Labavitch JM, Reid DS, Bennett AB: Transgenic overexpression of expansin influences particle size distribution and improves viscosity of tomato juice and paste.
J Agric Food Chem 2003, 51:7465-7471.
Overexpression of EXPA1 in tomato fruit leads to higher-viscosity tomato paste and juice with larger particle size, perhaps as a result of increased cell-wall hydration.PubMed Abstract | Publisher Full Text
Yoo SD, Gao ZF, Cantini C, Loescher WH, van Nocker S: Fruit ripening in sour cherry: changes in expression of genes encoding expansins and other cell-wall-modifying enzymes.
J Am Soc Hortic Sci 2003, 128:16-22.
Expression of four EXPA genes is strongly upregulated upon onset of fruit ripening in the sour cherry.
Harrison EP, McQueen-Mason SJ, Manning K: Expression of six expansin genes in relation to extension activity in developing strawberry fruit.
J Exp Bot 2001, 52:1437-1446.
Developmental analysis of expression of six EXPA genes in strawberry fruit indicates distinctive roles for the different genes.PubMed Abstract | Publisher Full Text
Obenland DM, Crisosto CH, Rose JKC: Expansin protein levels decline with the development of mealiness in peaches.
Postharvest Biol Technol 2003, 29:11-18.
Downregulation of EXPA gene expression in pear fruit is associated with onset of mealiness.Publisher Full Text
Gray-Mitsumune M, Mellerowicz EJ, Abe H, Schrader J, Winzell A, Sterky F, Blomqvist K, McQueen-Mason S, Teeri TT, Sundberg B: Expansins abundant in secondary xylem belong to subgroup a of the alpha-expansin gene family.
Plant Physiol 2004, 135:1552-1564.
Analysis of expansin gene expression indicates that certain EXPA genes are involved in secondary wall formation.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Belfield EJ, Ruperti B, Roberts JA, McQueen-Mason SJ: Changes in expansin activity and gene expression during ethylene-promoted leaflet abscission in Sambucus nigra.
J Exp Bot 2005, 56:817-823.
Transcripts of two EXPA genes accumulate specifically in leaf abscission zone during ethylene-stimulated leaf abscission.PubMed Abstract | Publisher Full Text
Chen F, Bradford KJ: Expression of an expansin is associated with endosperm weakening during tomato seed germination.
Plant Physiol 2000, 124:1265-1274.
An EXPA gene is expressed specifically in the endosperm region that is selectively loosened to allow emergence of the root from the seed.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Pezzotti M, Feron R, Mariani C: Pollination modulates expression of the PPAL gene, a pistil-specific beta-expansin.
Plant Mol Biol 2002, 49:187-197.
Documents expression of an EXPB mRNA in the secretory zone of the stigma and in the epidermal layer of the placenta.PubMed Abstract | Publisher Full Text
Balestrini R, Cosgrove DJ, Bonfante P: Differential location of alpha-expansin proteins during the accommodation of root cells to an arbuscular mycorrhizal fungus.
Planta 2005, 220:889-899.
Evidence by immunolocalization experiments that α-expansins are involved in cell-wall remodeling during formation of the mycorrhizal infection.PubMed Abstract | Publisher Full Text
Giordano W, Hirsch AM: The expression of MaEXP1, a Melilotus alba expansin gene, is upregulated during the sweetclover-Sinorhizobium meliloti interaction.
Mol Plant Microbe Interact 2004, 17:613-622.
Identified an EXPA gene in a legume (sweetclover) that is upregulated during Sinorhizobium-induced nodule formation.PubMed Abstract
O'Malley RC, Lynn DG: Expansin message regulation in parasitic angiosperms: marking time in development.
Plant Cell 2000, 12:1455-1465.
When Striga switches to its parasitic mode, three EXPA genes are activated and are involved in developmental changes that include formation of the root-like parasitic organ (haustorium).PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Wrobel RL, Yoder JI: Differential RNA expression of alpha-expansin gene family members in the parasitic angiosperm Triphysaria versicolor (Scrophulariaceae).
Gene 2001, 266:85-93.
The authors identified EXPA genes that are upregulated in the parasitic plant Triphysaria by cytokinin application; they are probably not involved in haustorium development.PubMed Abstract | Publisher Full Text
Jones L, McQueen-Mason S: A role for expansins in dehydration and rehydration of the resurrection plant Craterostigma plantagineum.
FEBS Lett 2004, 559:61-65.
Wall extensibility and expression of an EXPA gene are markedly increased during dehydration and rehydration of this resurrection plant.PubMed Abstract | Publisher Full Text
Ridge I, Osborne DJ: Wall extensibility, wall pH and tissue osmolality: significance for auxin and ethylene-enhanced petiole growth in semi-aquatic plants.
Plant Cell Environ 1989, 12:393.
Submergence-induced and ethylene-induced elongation of aquatic plants depends on cell-wall acidification.
Cho HT, Kende H: Expression of expansin genes is correlated with growth in deepwater rice.
Plant Cell 1997, 9:1661-1671.
Expression of certain EXPA genes in rice is increased upon submergence-induced internode elongation.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Vreeburg RA, Benschop JJ, Peeters AJ, Colmer TD, Ammerlaan AH, Staal M, Elzenga TM, Staals RH, Darley CP, McQueen-Mason SJ, Voesenek LA: Ethylene regulates fast apoplastic acidification and expansin A transcription during submergence-induced petiole elongation in Rumex palustris.
Plant J 2005, 43:597-610.
In Rumex, a leafy herb adapted to wet conditions, submergence increases ethylene concentration which promotes RpEXPA1 expression and apoplastic acidification, which combine to stimulate growth.PubMed Abstract | Publisher Full Text
Colmer TD, Peeters AJ, Wagemaker CA, Vriezen WH, Ammerlaan A, Voesenek LA: Expression of alpha-expansin genes during root acclimations to O2 deficiency in Rumex palustris.
Plant Mol Biol 2004, 56:423-437.
An analysis of EXPA gene expression, showing selective expression of certain genes in root tips, cambium and other cells involved in plant growth.PubMed Abstract | Publisher Full Text
Cho HT, Kende H: Expansins in deepwater rice internodes.
Plant Physiol 1997, 113:1137-1143.
Purification and partial characterization of two EXPA proteins from rice internodes.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Cosgrove DJ, Li LC, Cho HT, Hoffmann-Benning S, Moore RC, Blecker D: The growing world of expansins.
Plant Cell Physiol 2002, 43:1436-1444.
A review of expansin gene expression and its actions on the plant cell wall.PubMed Abstract | Publisher Full Text
Zhang N, Hasenstein KH: Distribution of expansins in gravi-responding maize roots.
Plant Cell Physiol 2000, 41:1305-1312.
By fluorescence immunolocalization, EXPA signals in the cell wall were shown to become stronger on the upper side of roots bending downwards during gravitropism.PubMed Abstract | Publisher Full Text
Im KH, Cosgrove DJ, Jones AM: Subcellular localization of expansin mRNA in xylem cells.
Plant Physiol 2000, 123:463-470.
EXPA mRNAs show polar localization to the apical or basipetal ends of xylem cells.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Li LC, Cosgrove DJ: Grass group I pollen allergens (beta-expansins) lack proteinase activity and do not cause wall loosening via proteolysis.
Eur J Biochem 2001, 268:4217-4226.
Using five protease assays, this study tests the idea that grass pollen EXPBs (group-1 allergens) loosen cell walls via proteolytic action; the results refute this idea.PubMed Abstract | Publisher Full Text
Li LC, Bedinger PA, Volk C, Jones AD, Cosgrove DJ: Purification and characterization of four beta-expansins (Zea m 1 isoforms) from maize pollen.
Plant Physiol 2003, 132:2073-2085.
A detailed biochemical characterization of the physical properties and action of maize group-1 pollen allergens (EXPBs).PubMed Abstract | Publisher Full Text | PubMed Central Full Text
McQueen-Mason SJ, Cosgrove DJ: Expansin mode of action on cell walls. Analysis of wall hydrolysis, stress relaxation, and binding.
Plant Physiol 1995, 107:87-100.
A detailed biochemical and biophysical analysis of how α-expansins loosen plant cell walls.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
McQueen-Mason SJ, Fry SC, Durachko DM, Cosgrove DJ: The relationship between xyloglucan endotransglycosylase and in vitro cell wall extension in cucumber hypocotyls.
Planta 1993, 190:327-331.
Presents evidence that expansins do not have xyloglucan endotransglycosylase (XET) activity and that XET lacks wall extension activity, either by itself or in the presence of xyloglucan oligosaccharides.PubMed Abstract | Publisher Full Text
Yuan S, Wu Y, Cosgrove DJ: A fungal endoglucanase with plant cell wall extension activity.
Plant Physiol 2001, 127:324-333.
The first demonstration that plant cell walls extend as a result of hydrolysis by an endoglucanase (fungal not plant); the characteristics of this extension are very different from that caused by expansin action.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
McQueen-Mason S, Cosgrove DJ: Disruption of hydrogen bonding between plant cell wall polymers by proteins that induce wall extension.
Proc Natl Acad Sci USA 1994, 91:6574-6578.
This report shows that α-expansin loosens paper made of pure cellulose, without hydrolysis, and proposes a model for expansin action involving local dissolution of the adhesion of matrix polysaccharides to the surface of cellulose microfibrils.PubMed Abstract | Publisher Full Text | PubMed Central Full Text
The Institute for Genomic Research [http://www.tigr.org] webcite
The Institute for Genomic Research (TIGR) website.
Weblogo [http://weblogo.berkeley.edu] webcite
WebLogo is a web-based application for the generation of sequence logos (graphical representations of amino acid or nucleic acid multiple sequence alignments).
Salle de Modelisation et d'Imagerie [http://smi.snv.jussieu.fr] webcite
The DrawHCA program available at this site allows the construction of HCA plots.
Callebaut I, Labesse G, Durand P, Poupon A, Canard L, Chomilier J, Henrissat B, Mornon JP: Deciphering protein sequence information through hydrophobic cluster analysis (HCA): current status and perspectives.
Cell Mol Life Sci 1997, 53:621-645.
A summary of the interpretation of HCA plots.PubMed Abstract | Publisher Full Text


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Camp Necessity La.
Grand Ecore & Texas Road
Aug. 31st 1844
Your two letters of July and August have just been recieved and read you can scarsely immagine with how much pleasure. I have waited so long for an answer to my three letters (I have written you three times Julia one of them you probably had not time to get when you wrote yours) that I began to dispare of ever recieving a line from you; but it come at last and how agreeable the surprise! Take example in punctuality by me Julia, I have rec'd your letters only to day and now I am answering them. But I can forgive you since the tone of your last letter, the one in pencil, is so conclusive of constancy. I am sorry to hear that Mrs. Dent thinks there is nothing serious in our engagement with me nothing is more serious or half as pleasant to think of—Since the arrival of your letters I have read them over and over again and will continue to do so until another comes. I have not been into Camp Salubrity yet to deliver to Mr. Hazlitt verbally the messages you sent him, but I wrote him a note this


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Natural Selection 2 is the sequel to the popular Half-Life mod Natural Selection. It is built on our own engine and will be distributed through Valve's Steam platform.
Dec 5th, 2013 0 people agree 0 people don't
I would have given the game a 10 but i just made this account to rate the game and it wouldn't let me give it anything higher then an 8 because my account was to new. this game deserves a 10
If you liked:
natural selection 1
savage 1 and 2
command and conquer renegade
You will like this game.


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Does it get any worse than this for President Obama?
The first Gallup poll out since the president's make-or-break jobs speech show only 45 percent of Americans approve of his jobs bill and still there isn't much faith it will actually help the economy. How about the reaction of Democratic lawmakers like Sens. Bob Casey Jr. of Pennsylvania, Joe Manchin of West Virginia and Mary Landrieu of Louisiana, who already complained about it on the record? And notice Senate Majority Leader Harry Reid (D-Nev.) isn't rushing to schedule it for a vote on the Senate floor, which he controls.
Then the Democrats lose former Rep. Anthony Weiner's seat Tuesday in a Democratic stronghold in New York's 9th district in Queens. Badly. By eight points. It was a referendum on President Obama and proved that disappointment, frustration and anger Democrats feel toward Obama go well beyond the troubled economy to Israel and other issues.
On Wednesday the House held a hearing into the collapse of Solyndra, the too-tight-with-the-administration solar panel company floated a $535 million loan guarantee from the Department of Energy before going belly-up. It is nothing short of a public-relations disaster for the White House.
Thursday things plunged into newly treacherous territory for the president. James Carville, Democratic strategist and loyalist through and through, felt the need to come out in a CNN op-ed and tell the White House, and the rest of the world, that it's time for Team Obama to panic. Literally. "The time has come to demand a plan of action that requires a complete change from the direction you are headed," wrote Carville. "It's not going to work with the same team, the same strategy, and the same excuses."
Perhaps if President Obama had received Carville's warning on Wednesday, he would have stopped himself before a crowd in North Carolina from exhorting, "If you love me, you've got to help me pass this bill." He is the president of the United States and right now only his family and friends — and not one remaining Democratic constituency group — loves him.
It won't be possible to turn things around if the president and his team don't grasp how untethered they are from the country right now.
PERRY JUMPED 9 POINTS IN FLORIDA AFTER THE CNN DEBATE — CAN ROMNEY STOP HIM? Ask A.B. returns Tuesday, Sept. 20. Please join my weekly video Q&A by sending your questions and comments to firstname.lastname@example.com. Thank you.
Panic time for the White House
Does it get any worse than this for President Obama?


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Could Damon be innocent?
It is possible that another vampire used his mind powers to make him think it was Damon who killed Alaric wife. He doesn't have many fans and it does seem like allot of people hate the Salvador brothers and knew Katherine quite well. Perhaps they may have even been jealous or even blame Damon for what happened to Katherine.
If it turns out that Alaric is Elena's father or his wifes child then things could get quite sticky. I guess we will have to wait to see what Damon says about it when it all comes to light, but I wouldn't be surprised if Damon didn't do the deed as it would kill any chance of him been with Elena or Bonnie.


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On February 12th, 2015 during the annual meeting of De Groene Zaak, the report "Governments Going Circular" was launched. It is the result of a global scan in search for governments that stimulate the transition towards a circular economy. Accessible through an interactive website, the best practices are a source of inspiration for governments alike and set the example for thought leadership.
In recent years, it has become increasingly apparent that the global economic system is imposing an increasing strain onessential natural resources. Although resources do not yet face complete depletion, the economical available proportion of these reserves will, sooner or later, be exhausted. With this in mind, many companies started to develop and apply circular business models, rather than the traditional linear, 'end-of-life' model. Read the rest of this entry »Read the full article


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Sacramento Law Group was created to "help people in the greater Sacramento area get out of debt and get a fresh financial start" says Sacramento bankruptcy attorney Adam Garcia. "Sacramento Law Group is beginning the new year by helping people who had a rough financial year in 2012 get a fresh start in 2013."
How does Sacramento Law Group help people get a fresh start in the new year? "The firm helps people file for chapter 7 or chapter 13 bankruptcy with the desired result being the discharge of debt, cessation of collection efforts, and restoration of financial stability."
From stopping bill collectors to discharging overwhelming debt, Sacramento Law Group can help people in the greater Sacramento area achieve meaningful debt relief. To learn more about Sacramento Law Group visit their website at http://sacramentolawgroup.com/
Sacramento Law Group, based in Sacramento, California, is a bankruptcy firm that provides consumer bankruptcy services to individuals in financial difficulty. Visit the firm's website to learn more about the firm and its services.
Sacramento Law Group helps people file for bankruptcy relief under the bankruptcy code. As a bankruptcy firm, Sacramento Law Group is a debt relief agency whose services, benefits, and assistance may involve bankruptcy relief under Title 11 of the United States Code. Bankruptcy Attorney Adam Garcia is responsible for this press release. Advertisement.


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- Jessa Duggar shares a rare photo of her brother Josh Duggar's daughter Meredith - US Weekly
- Michael Lohan's estranged wife Kate Major is detained by cops at an airport - TMZ
- Is there something between Ben Affleck and Halle Berry? - Gossip Cop
- The Hunger Games author Suzanne Collins says goodbye to the popular series after the final movie is released - Just Jared Jr
- What happened backstage with Kylie Jenner at The Ellen Show? - Radar
- Bryan Cranston tears up over a Breaking Bad memory - Huffington Post
- Leonardo DiCaprio's Oscar race heats up - Lainey Gossip
- The Captain America: Civil War movie may be better than the comic book - The Hollywood Reporter
26 July 2007 at 6:34 AM
Fashion Faceoff: Emanuel Ungaro Dress
Posted in Fashion Faceoff: Emanuel Ungaro Dress
About this photo set: Catherine Zeta-Jones looked stunning in a red spangled-sequin starburst microdress by Emanuel Ungaro at the NY premiere of No Reservations on Wednesday. The 37-year-old… Read More Here
Photo #: 507521
Posted to Catherine Zeta Jones, Fashion Faceoff


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America's First Choice In Restoration
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- Home
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Biker Business Card Holder
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Product Description
Our friends at H&K Steel Sculptures have done it again with this great Biker Business Card Holder! Perfect to display in the office, at the register, or anywhere in your business.
The moment you hold an H&K Steel Sculpture, you'll feel the weight and solidity, see the craftsmanship and appreciate its quality and value.
Dimensions: All H&K Business Card Holders are 5-7" Tall.
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Picture: Robert De Niro, Jessica Alba and Ben Stiller The World Premiere of 'Little Fockers' held at the Ziegfield Theatre - Arrivals New York City, USA...
Robert De Niro, the legendary Hollywood actor and star of new comedy movie 'Little Fockers' is set to make his first appearance on 'The Late Show with David Letterman' after taping an interview that will air on Christmas Eve, reports CBS News. During the chat, the 67-year-old was teased by Letterman for his previous reluctance to appear on the show.
In the interview, which also features De Niro's co-star Dustin Hoffman, Letterman asks the 'Taxi Driver' star what took him so long to appear on the program, saying, "We've been on the air for 30 years. Right now, we're on CBS. We used to be on NBC. I live in your neighbourhood, literally, your neighbourhood. We're neighbours, and this is your first time", before asking "What have I done?" The actor passed the blame on to his co-star, saying, "Oh, God, I don't know. I just, well, whatever. Dustin, he'll explain to you", to which Hoffman replied, "I can maybe answer for him. I am the De Niro Whisperer".
De Niro's new movie 'Little Fockers' is the third film in the 'Meet the Parents' comedy franchise and sees Ben Stiller, Owen Wilson, and Hoffman reprise their roles as members of the dysfunctional families. The film opened in cinemas worldwide yesterday (22nd December 2010).


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Facebook fends off telecom firms' complaints
Facebook founder Mark Zuckerberg fended off complaints on Monday that the hugely popular social network was getting a free ride out of telecom operators who host its service on smartphones.
Facebook founder Mark Zuckerberg fended off complaints on Monday that the hugely popular social network was getting a free ride out of telecom operators who host its service on smartphones.
China Telecom Ltd., one of the country's three main state-owned carriers, said Wednesday its profit rose 11.8 percent in the first half of the year as its Internet and mobile data businesses grew.
State-owned phone company China Unicom said Thursday its first-half profit jumped on higher mobile data revenue and lower fees to connect to a rival's network.
Mobile wallet service Isis is changing its name to distance itself from an acronym used to refer to militant group Islamic State of Iraq and Syria.
US telecom service firms Sprint and T-Mobile were nearing a deal valued about $32 billion and likely to be scrutinized by anti-trust regulators, US media reported Wednesday.
French conglomerate Bouygues offered 10.5 billion euros ($14.4 billion) for control of mobile operator SFR telecommunications, launching a bidding war Thursday that could extend the wireless industry's attempts to consolidate ...
Somalia's al-Qaida-linked group has ordered telecom companies to shut down mobile internet services over fears the U.S. can use the data to target militants.
China has issued licences for "fourth generation", or 4G, mobile phone services, the government said Wednesday, in a move to boost economic growth and job creation.
Nokia's shareholders were set Tuesday to vote on a historic decision to sell the Finnish company's mobile business to Microsoft, as the once-proud Nordic brand struggled to regain its edge.
State-owned China Telecom, the country's third-biggest mobile phone operator by subscribers, said Monday that quarterly profit grew by a fifth as it continued to benefit from spending by new users of data-hungry iPhones.


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Rock Shop Rehearsal Studios
Phone: 703-547-7454
Contact Us
Homestead website templates
Create a website today
Performance Rehearsal Space
If you're looking for high quality equipment and amazing sound, you've come to the right place. We are conveniently located in Tysons/Vienna right off 267, Rt. 7 and 495. 8455 R Tyco Road Vienna VA 22182. One block from Silver Line Metro - Springhill Stop. Free on site parking.
Rock Shop studios is a huge space (the stage is 25ft x 15 ft) with all the amenities your band needs. We have heat/AC, bathrooms, and great equipment. It has a rec space with table, chairs, air hockey table, foosball and vending machine with drinks and snacks. We have a 5 piece drum kit, 1 Marshall Valvestate AVT 275 2x12 amp, 1 korg m50 workstation,1 Korg 88 key digital piano, 1 mackie 10 channel board, sm58 mics, vocal monitors, 2 vox ac30 2x12 amps, 1 bass amp with ampeg head.
Studio Rental Pricing:
$30 per hour plus a 2.9% service fee
Studio Rental Requests:
Lessons in Voice, Guitar,Drums - Lessons in Vienna/Tysons and Ashburn
: Have a professional look at what you do...from your stage show to the way you package and present yourself, and provide you ideas on how to make your act stand out. We can help you fine tune your music. Working together we can make your "act" as professional and polished as possible and highlight your unique style and sound. Pat Sommers who is a seasoned recording and performance artist who has recorded and toured with such Rock n; Roll legends as The Who, Roger Daltrey (Rocks in the Head) and Kiss's Ace Frehley (Trouble Walkin, 12 Picks, Loaded Deck, and Greatest Hits Live) just to name a few. Pat Sommers has recorded and performed professionally as Lead Singer, lead guitarist and drummer and is musically proficient in all instruments in a Rock Band.
Rock Shop Studios also runs a hands-on music workshop specializing in performance and song writing. The teen program is run by Pat Sommers. Members are placed in bands that work together as a team to create a set list of original music and covers.The set list is based on the interests and talents of each band. Teen musicians can check out our band program at
Click to read more about the Pat Sommers' Rock Shop Teen Program:
Fairfax Times - It's all about the music in Vienna
Pat Sommers' Rock Shop helps musicians hone their craft
Northern Virginia Magazine - Vienna Kids Hit the Big Time
Patch.com - Pat Sommers Rock Shop at Viva Vienna
Rock Shop Studios Open by appointment:
8455 R Tyco Road 703-547-7454
Vienna Va 22182 Email : email@example.com
Studio Rental Requests:


text:
Now Playing
Attorney General Grilled On Security Leaks
Attorney General Eric Holder found himself in a familiar position Tuesday — up on Capitol Hill and under fire from Senate Republicans. One senator called on Holder to resign. Others on the Judiciary Committee wanted to know whether his Justice Department could properly pursue leak investigations — and why he wouldn't share more information about a botched gun operation.


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- ParaguayRetailFunding via Fundación Paraguaya
MartinaParaguayRetailFunding via Fundación Paraguaya
Virgen Del ...ParaguayRetailFunding via Fundación Paraguaya
La Amistad Group
One of the members is Señora Elva A., who prepares different types of jams in great quantities. She buys the fruits and prepares the jams and then goes around to a number of businesses where they offer her products. In addition, she has a business where she sells toys, clothing, and household utensils. She is a very hard-working woman and provides for her children in the best way possible. She is applying for this loan to purchase containers of different sizes because she wants to package her products for better presentation and handling.
Ellas están trabajando de forma unida para generar mas ingresos para sus familias.
Una de las integrantes es la señora Elva A. quién se dedica a elaboración de diversos tipos de dulces gran cantidad.
Ella compra las frutas y prepara los dulces y luego recorre varios negocios donde ofrece sus productos.
Además tiene un negocio donde vende juguetes, ropas, y utensilio para el hogar.
Ella es una señora muy trabajadora y mantiene a sus hijos de la mejor manera posible.
Solicita el préstamo para la compra de envases de diversos tamaños ya que desea envasar sus productos para una mejor presentación, y manejo de los dulces.
Additional Information
About Fundación ParaguayaFundación Paraguaya is a financially self-sustaining social enterprise that promotes entrepreneurship in urban and rural areas through microfinance, education programs, and more. Its focus on agriculture has helped many farmers boost their crop yields. And a number of other borrowers have taken out loans to start and grow social businesses that have helped deliver eyeglasses, medicines and other helpful services in their local communities.
This is a Group Loan
In a group loan, each member of the group receives an individual loan but is part of a larger group of individuals. The group is there to provide support to the members and to provide a system of peer pressure, but groups may or may not be formally bound by a group guarantee. In cases where there is a group guarantee, members of the group are responsible for paying back the loans of their fellow group members in the case of delinquency or default.
Kiva's Field Partners typically feature one borrower from a group. The loan description, sector, and other attributes for a group loan profile are determined by the featured borrower's loan. The other members of the group are not required to use their loans for the same purpose.
About Paraguay
33View loans »
Success!! The loan was 100% repaid
- Repayment Term
- 5 months (Additional Information)
- Repayment Schedule
- Monthly
- Pre-Disbursed:
- May 20, 2010
- Listed
- Jun 16, 2010
- Currency Exchange Loss:
- Covered
- Ended:
- Sep 15, 2010


text:
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You tired to make us "vodka tacos". Which was just you dipping pitas in vodka.
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Well. FUCK YA. But that's beside the point
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How the fuck do some people not know how to spell "yeah"? Jesus Christ.
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Forget password?
Remember Me
Towards the end of a cover of Simple Minds's "Don't You (Forget About Me)," Bayliss
stepped to the mic and reflected on the last 16 years since the band formed. It's been a
long road from the days
when UM performed in front of a handful of fans at small bars to Saturday's show at the
2,200-capacity Fillmore. Brendan talked about that long road and told the crowd that
everyone experiences self-doubt at some point. He ended his motivational talk by telling
the audience "everything will work out in the end."
Watch a montage of Mike Mirro photos with "Don't You (Forget About Me)," including
Bayliss's speech, as the soundtrack:
Here's the text of Bayliss's speech:
"You know Detroit, looking out tonight, seeing so many people...words cannot express
how good it feels to be onstage playing live music after so many years together we've been
doing this. I don't even know how many, it's like 16 or something. Way back when we were
in college we were starting this thing and it was like, when you're in college you're a
kid, you have no idea what the fuck you're supposed to do with the rest of your life, but
they tell you you have 4 years to figure it out and you gotta do it well, right?
You know when we started, we were playing bars and 15, 16, 20 people would come out and
we'd make about $18 and we were full of self doubt and we had no idea that it was going to
get to this point where we are right now. And so I want you guys to know when you're full
of doubt and you have no idea what's going on in your life and you're down and you're out,
you're not thinking anything is going anywhere, it happens to everybody, everybody in this
room is in the same position. We all have no fucking clue what is going on. So just
believe and have faith because everything will work out in the end. It works out for me,
it works out for him, it works out for him, it works out for her, for you, for you, for
him, for everybody." - Brendan Bayliss
Brendan gave a similar speech during UM's debut of the cover on December 30.
[Transcription via Amy McMenemy]
UPDATE 2/4 10:20 a.m. PT: Umphrey's has shared a video containing a slide show that
features Mike Mirro set to audio of "Don't You (Forget About Me)" from Detroit that
includes Bayliss's speech.


text:
Have you heard about Giving Tuesday? This new movement called Giving Tuesday is an event that happens on the Tuesday after Thanksgiving. This event was inspired in order to create a nationally recognized day that would kick off the season for giving. It was intended to be a tremendous response towards consumerism and commercialization after… Read More »
30 Inspirational Quotes About Gratitude
As we celebrate the Thanksgiving holiday with our friends and families, let's remember that Thanksgiving is about gratitude. Here is a great collection of quotes about gratitude that remind you of just how much you have to be thankful for. "Gratitude is the inward feeling of kindness received. Thankfulness is the natural impulse to express… Read More »
23 Things To Be Thankful For In Life Every Single Day
Thanksgiving has always been one of my favorite holidays. I love getting together with family and friends and sitting down together to eat a big meal. I also love the fact that on this day, people take the time to give thanks. I've always wondered though, why people wait until Thanksgiving to express gratitude. Thanksgiving… Read More »
7 Great Quotes About Autumn
Autumn is a time of change and transformation. Its beauty can be seen in all aspects of the surrounding environment, ranging from the stunning, colorful changes in the fall foliage to the refreshing, crisp weather. There is no shortage of activities to enjoy in this vibrant time of the year, many of which revolve around… Read More »
10 New Year's Quotes to Inspire You
With every new year, people always have new goals that that they wish to accomplish. Here are some inspirational quotes for the new year to help you in reaching those goals. "Cheers to a new year and another chance for us to get it right." ~Oprah Winfrey "Write it on your heart that… Read More »
10 Lovely Quotes About Spring
It's finally starting to feel like spring here. We've had a long cold winter here in NJ. After being confined to the indoors for so long, I am ready to head outdoors to do some gardening, and to just enjoy the warmer weather. Check out this list of 10 lovely quotes about spring I compiled. Do… Read More »
Have a Great Weekend.
The past few days were really hot and steamy around here so we plan on keeping cool this weekend. Philip's cousins from Toronto will be in town so most likely there will be some sort of family gathering going on this weekend. What are your plans this weekend? Before you start your weekend, I wanted… Read More »
D is For Dr. Seuss – My Favorite Dr. Seuss Quotes
I don't really remember reading Dr. Seuss books when I was young. When I was pregnant with my son, I went crazy buying books for him, but when I came across the Dr. Seuss books, I held off on buying them because at first glance I thought they looked like a whole bunch of nonsense. … Read More »
A is for Admiration – 6 Inspirational Women I Admire
When it comes to role models, who do you admire? Some admire athletes, movie stars and singers. Not me. Instead, I admire people who have made a contribution to this world due to their hard work and perseverance. There are many women who fit that bill, but there's no way for me to list them… Read More »
{Love}ly Quotes for Valentine's Day
In honor of Valentine's day, I wanted to list some lovely quotes about love. Enjoy! Hope you enjoyed these Cute Quotes for Valentine's Day Happy Valentine's Day!
Inspiring Words of Wisdom
Here are a few inspiring words of wisdom to motivate you!


text:
Posted April 24, 2013 on AP on Fox
Chris Stewart reached career milestones of 100 goals and 100 assists and the St. Louis Blues clinched a playoff berth with two games to spare, beating the Colorado Avalanche 3-1 on Tuesday night. Brian Elliott had to make just 17 saves two nights after getting yanked in a 5-3 loss at Colorado that squandered the Blues' first chance at clinching. St. Louis, which entered the game needing a point to qualify, scored two power play goals for the first time in 32 games since Feb. 15 in the home-and-home rematch. Patrik Berglund and Andy McDonald beat Semyon Varlamov on consecutive shots in the second period to make it 3-0. Together, that duo had mustered two assists in 14 games. The Blues have made the playoffs in consecutive seasons for the first time since 2003-04. They've won four in a row at home to match their season best, taking the first three by 2-1 scores, and were in fifth place in the Western Conference and a point ahead of San Jose, at home against the Stars later Tuesday....
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But fear not - there is plenty to explore...Shop all The North Face Shop sale The North Face
Hey, maybe the words "Baker" and "Delux" seem a little incongruous if you've spent much time riding at Mt. Baker, but the touch of faux fur on the collar of The North Face Baker Delux Jacket is just to throw you off the scent (and it's removable) - this jacket's got the burly weatherproofing of HyVent® 2L fabric and 100 grams of Heatseeker™ insulation to keep you toasty in the most heinous winter weather. Go Delux and live a little, girl!
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Comments about The North Face Baker Delux Jacket - Women's:
I love the style. The fit is not quite right, not because of me but the sizing is off. I had to order a different size. I am not the first to say that about this jacket.


text:
Congratulations, Cara Buono!
According to Michael Ausiello, the actress - best known for roles on The Sopranos and Dead Zone - has landed the role of ER's final fatality. Most likely.
Buono character, Lisa, is a 36-year-old pregnant woman who goes into labor after getting into a car crash. An ER rep would only say that Buono is playing one of ER's "last trauma patients.
The ER series finale is scheduled to air on April 2.


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Tags: deep, throat, latina, beauty, cuban, ass, pounded, hard
Categories: Inter Racial
Submited: 6 years old
Views: 5566
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Section 2257
Deep throat latina beauty gets her cuban ass pounded so hard


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- Filed Under
SATURDAY: Ithaca (7-17, 2-11) at Alfred (17-7, 8-5)
WHEN: 2 p.m.
WHERE: McLane Center gym, Alfred
RADIO: None
ONLINE: bombers.ithaca.edu
LAST MEETING: Senior guard Thomas Tsatsis scored 17 points to lead the visiting Saxons past the Bombers, 94-67, on Jan. 10 at Light Gym. Senior Frank Mitchell led all scorers with 30 points for Ithaca.
LEADERS: For Ithaca, Frank Mitchell (16.8 ppg., 8.0 rpg.), Tom Sweeney (9.0 ppg.), Sam Bevan (6.8 ppg., 3.76 apg.). For Alfred, Thomas Tsatsis (13.3 ppg.5.3 rpg.), Tyler Seelman (10.8 ppg.), Steve Lothridge (6.3 rpg.).
FAST FACTS: Ithaca will close its season Saturday at Alfred; the Bombers qualified for the NCAA Division III tournament two of the previous three seasons, last year advancing to the Sweet 16 for the first time in program history. ... Alfred can clinch a berth in the Empire 8 tournament with a win over the Bombers.
SATURDAY: Ithaca (20-4, 12-1) at Alfred (6-18, 1-12)
WHEN: 4 p.m.
WHERE: McLane Center gym, Alfred
RADIO: None
ONLINE: bombers.ithaca.edu
LAST MEETING: Ithaca raced to a 46-23 halftime lead and cruised past the visiting Saxons, 91-48, on Jan. 10 at Light Gym. Senior forward Jenn Escobido led a balanced scoring attack for IC with 17 points, while junior guard Ally Mnich added 15.
LEADERS: For Ithaca, Mary Kate Tierney (13.4 ppg., 8.5 rpg., 3.5 apg.), Kathryn Campbell (11.5 ppg.), Jenn Escobido (11.0 ppg., 5.5 rpg.). For Alfred, Harley Cleary (15.2 ppg.), Emilea Harrison (8.5 ppg., 2.2 apg.), Sarah Federico (6.6 rpg.).
FAST FACTS: The Bombers, winners of 12 straight, are tied with Hartwick atop the conference standings, both at 12-1. The top finisher in the regular season hosts the conference tournament; if there is a tie, Ithaca would win the first tie-breaker (NCAA Div. III regional ranking). ... With a win, Ithaca is guaranteed to host next weekend's Empire 8 conference tournament.


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Alchemist are a 6 piece band from Auckland, NZ that defines themselves by their message, not their genre. Drawing influences from all over the place, Alchemist has a love for all music that inspires and hopes to give something back.


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Marcus Llewellyn Dowdell
Position: WR
Height: 5-10 Weight: 179 lbs.
Born: May 22, 1970 in Birmingham, AL
High School: Banks
College: Tennessee St. (school history)
Drafted by the New Orleans Saints in the 10th round (276th overall) of the 1992 NFL Draft.
Weighted Career AV (100-95-...): 4 (9734th overall since 1950)
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Output includes all games from 1920 to 2015.


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Originally Posted by thefizz
Actually I've seen Dinesh in action and its safe to say there will be plenty of beer left for the rest of us. ..
What can I say, I put on a pretty poor show in Dublin. Peter, come to Canada and I'll give it another go!
Originally Posted by Fintan
Dinesh get in training, you got 18 months before you can shut this dude up for good.
I'm starting as I type.
Kick his ass, Sea Bass!