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Those seeking to know more about their candidates are invited to attend the Greendale candidate forums hosted by the Greendale Chamber of Commerce and the League of Women Voters.
Two candidate forums will be held this year, one for Village Board candidates and the other for School Board candidates.
The Village Board candidate forum is slated from 6:30 to 8:30 p.m. March 21. The School Board candidate forum will be held from 6:30 to 8:30 p.m. March 27. Both forums will be held in the Greendale High School multipurpose room, 6801 Southway.
All candidates have been invited and are expected to participate. The candidates will answer audience questions posed by a mediator.
For information, call Todd K. Michaels at (414) 423-2100.
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- Greendale School Board Academy to be held for prospective spring election candidates
- Greendale police report: Nov. 26, 2015 issue
- Greendale trustees raise tax levy 1 percent, bump up budget by 45 percent to construct fire station (4)
- Greendale police report: Nov. 19, 2015 issue
- Greendale's first fire engine, which entered service in 1938, returns home (10)
- Greendale Marching Band holds fundraiser for parade trip
- Greendale Assessor's Office to photograph homes, businesses for 2016 study (2)
- Greendale police report: Nov. 12, 2015 issue
- Greendale's tree lighting ceremony scheduled for Nov. 27
- Santa Claus to arrive early at Southridge Mall this weekend


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Thursday, July 14th, 2005 by
How do I get my computer clock to display 24- hour time rather than 12-hour AM and PM?
I know we have many readers in Europe and I'm sure there's a few in the military or who just prefer going to bed at 22 o'clock every night. Well, this tip is for you.
First go to Start/Control Panel and look for "Date, Time, language & Regional… Continue reading

subset: commoncrawl


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This Is By Far The Best Way To Pick Up Your New BMW
Earlier today, I mentioned I was starting on a cross-country trip to LA to drop off a reader-spec'd BMW 2 Series to our contest winner. What I didn't mention was that the trip has sort of started already, since I went to pick up the BMW from their Greenville, SC factory. This is by far the best way to pick up a new… »


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Participate in This Event or find another event
Saturday, April 30, 2016
11 a.m.
Beaumont Civic Center
701 Main St , Beaumont, TX Map it
Already a participant?
Log in here
19 teams and 106 participants have raised $5,124.94
Total above may not reflect all participants, teams, and donations
T-Shirt Order
Due TODAY!
Please bring your orders and money to the American Cancer Society officee between 4pm and 7pm.
It's time to seel some t-shirts! We have our event theme t-shirt as well as our holiday t-shirt ready to be sold. Please download the forms below and share them with everyone. These shirts are sure to be something everyone will want!
They are $15 CASH and orders are due on November 9, 2015.
We will be at the ACS Office from 4-7pm on November 9th for you to drop off tshirt orders.
Event Theme Shirts - Purple shirt with the event logo for this year's event.
Event Theme Shirt - Individual Order Form
Event Theme Shirt - Team Order Form
Holiday Theme Shirts - Gray shirt with the holiday artwork on back and Relay For Life logo on front of shirt.
Holiday Theme Shirt - Individual Order Form
Holiday Theme Shirt - Team Order Form
Relay Keepsake (Christmas Ornament)
This holiday season, you can shine that light even farther when you share our limited edition Relay For Life keepsake.
The palm-sized metal keepsake, embellished with a Relay For Life logo and antique silver finish and strung with a decorative purple ribbon, can be yours with a $35 contribution made through the keepsake page on any local Relay For Life event website. Each keepsake measures 2.15 inches wide by 3 inches high. It comes with a customizable card in a specially packaged box.
Contributions will help Relay event participants reach their fundraising goals, and most importantly, help fund the American Cancer Society's mission to save lives. It counts toward you or your team, just have those purchasing search for your name to give you credit. (You MUST be registered for 2016).
For timely delivery for the holidays, order through November 7 while supplies last. Order a keepsake today.
Mammograms = Donation to Relay For Life!
For the month of October, The ODC will be donating $10.00/Mammogram done at the facility to Relay for Life or the American Cancer Society.
If you are due for your mammogram, please give ODC a call to schedule yours. There's is nothing better than taking care of yourself with preventative measures and supporting the American Cancer Society!
Thank you ODC!!
The American Cancer Society Relay For Life movement is the world's largest and most impactful fundraising event to end cancer. It unites communities across the globe to celebrate people who have battled cancer, remember loved ones lost, and take action to finish the fight once and for all. With the support of thousands of volunteers like you, the American Cancer Society is helping save more than 500 lives a day. Please join us and take action against a disease that has taken too much!
Remember or honor a loved one who has battled cancer with a luminaria tribute bag »
Connect with others and find resources to help you participate in the Relay For Life movement »
Event sponsored by:
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My gyno called yesterday to tell me that the results from my Pap came back abnormal. Something about low grade cells...They already scheduled a colposcopy for me which I thought was unusal since normally they'd do a follow up pap first. I know abnormal results could be a number of things and I shouldn't be too concerned yet, just wondering if anyone else had this procedure done? Was it painful?
Abnormal Pap
Comments (5)
So first off, don't get worked up yet. There are a number of reasons why you can have abnormal PAPs. The colposcopy is not too bad. A little pinch that for me did not seem any worse than a regular pap but maybe a little more cramping after. The LEEP (to remove abnormal cells) is a bit more uncomfortable. I have had 2 colps and 1 LEEP done. It is not comfortable and you should plan on no intercourse until you feel okay with it, but the colp was not as bad as I expected. I had Grade 3 abnormal cells (1 level from cancer) and they were able to get all of the abnormal tissue with the LEEP. I had another abnormal PAP a few years later, had a colp and it was grade 1 abnormal, so no LEEP. You really can have an abnormal PAP for so many benign reasons though. Good luck and God Bless. I know it is scary to find out anything like that but this is exactly why as women we get PAPs done, to catch anything off early on.
Benjamin & Celeste = Grace 5-13-10 Isabella 8-31-11 Alexandria 10-17-13, Benjamin Levi 8/7/15, ^I^ 10-15-14, Loving God's Blessings,
I had an abnormal PAP one time in my early 30's, now in my early 40's and haven't ever had another one. So take a deep breath and don't worry too much. There are lots of reasons they can be abnormal. They did the Colp and found there to be nothing wrong. During the process it did hurt somewhat and I was light headed when standing up, directly after the procedure.
Lisa (43)
Married to Bill (41) since 8/08/09
Elizabeth Joy 6/1/10, 7lbs 11oz, 21 inches
I had an abnormal pap when I was 22. Instead of doing the colo I went back in 5 months. The follow up pap was fine. Last year I had another abnormal pap and this time they scheduled the colo. That came back fine.
Pain is relative - I thought the colposcopy hurt like hell.
Abnormal paps can be caused by a LOT of things, many of which are no big deal, so try not to worry.
Playing with scissors since 1998.
Making kids boutique clothes and trying to stay sane.
Kylie 6.23.10
Colton EDD 6.4.13


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I'm trying to kill two birds with one stone - I'd like to make some dresses for my daughter and I want to learn how to make button plackets.
She has a couple RTW dresses with 3-5 buttons up the center back. I though if I could find a similar pattern I could practice my buttons on something wearable, but not too important. Any suggestions?
BTW - I need a pattern with instructions, not BWOF or the like
Heirloom patterns almost always use this type of closure. Try patterns from "Children's Corner", Martha Pullen, and others. Google "heirloom sewing patterns". Picking up a copy of Sew Beautiful magazine at the local chain will give you many heirloom pattern makers.
Try Oliver + S patterns. I have the Family Reunion Dress and the Music Box Jumper and both button up the back. Also, their instructions are marvelous and the results are always nice.
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Phlox and Archer's actions in Dear Doctor
View Single Post
November 20 2011, 06:52 PM
The Overlord
Re: Phlox and Archer's actions in Dear Doctor
horatio83 wrote:
sonak wrote:
Wow, you responded to my post using LITERALLY the exact two arguments I just wrote about, which are both just sloppy slippery slope fallacies.
Well done, sir. That couldn't have worked out better if it was done intentionally.
I especially liked the "you'd HAVE to go to war with two to three powers!" part, that was funny.
because of course giving a cure for a people who ask for it is JUST LIKE fighting massive wars of liberation.
how can I attack logic like that?
You obviously can't with your big lettered words and your lack of an argument.
But you can try to explain why one should help a sick species but not a subjugated species. You might wanna pick up Overlord's point about genocides like in Rwanada to get something going.
The Overlord wrote:
The Cold War is not the same as a plague ravaging a population, the Cold War could have resulted in mass death, but didn't this plague did. As for WWIII. You are forgetting that the amount of effort Phlox and Archer had to put in to stop the plague was minimal compared the effort the Vulcans could have needed to stop WWIII.
Phlox and Archer got involved, they made the cure and then they decided not to give the cure, the broke the PD by getting involved in the first place. Heck the PD didn't even exist back then, so there was nothing really stopping them from getting involved in the first place. They fact they got involved, give the Valakans false hope and then decide not to give the cure to them. That comes off as extremely cruel and callous. As was mentioned before, this is like someone with a jug of water coming across a man dying of thirst and decides not give any of his water to the man and then says that was the moral thing to do.
I think the difference between us is that you talk about cruelty, psychopaths and morals whereas I talk about ethics.
Let me phrase it like this, I agree with you that what the Vulcans or Archer did was cruel or even sociopathic (only so if the society we talk about stands alone, not embedded into a world with many alien species) but it was also the ethically right thing to do. This sounds like a contradiction but I don't think it is. To kill someone is normally morally and ethically wrong whereas to kill Hitler might have been morally wrong but if there was any ethical injunction for my grand-grand-parents it was to kill this man.
Except I don't see the actions of Phlox and Archer akin to killing Hitler to stop the Holocaust, I see more akin to letting the entire German population die because of the Holocaust. I don't see the ethics in Phlox and Archer's actions, because you seem to suggest that all Valakans are responsible for the mistreatment of the Menk, not just the leaders. Should Valakan children be left to die because of the sins of their fathers? I do not think collective punishment is moral or ethical. If Archer and Phlox gave the Valakans the cure, perhaps they would have been so grateful that they would have stopped mistreating the Menk right away, if Phlox and Archer merely suggested it. That would kill 2 birds with one stone, literally a win-win scenario, instead Valakan children are dying in the streets because Phlox used Nazi like pseudo science to justify not helping them.
The problem with this episode is it makes Phlox and Archer extremely unlikable to a large portion of their audience and that was not good for a TV series struggling for ratings.
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As you head out to shop for the holidays this weekend -- if you're so inclined -- be sure to swing by a record store for the Autumn version of Record Store Day. There are a number of special releases dropping on 11/27 to celebrate the occasion. From that pack, I thought I'd spotlight a supplementary EP from the glorious Rhiannon Giddens, who recorded more songs for her solo debut, Tomorrow Is My Turn, than she needed. So, she and producer T Bone Burnett collected the remainders for an EP titled Factory Girl. If you loved Tomorrow, staff reviewer Jerome Clark thinks this one will knock you off your feet too. Rounding out this week's picks are a two-disc collection from Neil Young and a little holiday burst of brightness from the Felice Brothers. If the Christmas music must start now, let's do our best to make it rootsy.
Oral History of No Depression / Jason Isbell hits his stride / Dave Rawlings Machine / Greensky Bluegrass, Infamous Stringdusters, and Yonder Mountain Stringband / Punch Brothers, I'm With Her, and a New Generation of Stringbands
Jay Farrar / The Mavericks / Lucinda Williams / Madisen Ward & the Mama Bear / Lee Bains III & the Glory Fires ... and much more!
Backstage at ND Roots venues / On the road with Amy Speace / Why Banjo? / Where Songs Live / Behind the scenes with Guy Clark and friends / Stringband Family Tree


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Recent News
Ferris360. We are not just another gym, we offer our own signature carefully crafted Ultimate 60 Minute Full Body Workout! Our group fitness classes allow you to exercise and be motivated by others and yet the workouts are still very individualized. As one client said, "it is like having a personal trainer for a fraction of the price".
Learn about our unique approach
A Kidney Donor's Fitness Journey
Client Testimonial
"I started at Ferris360 in July 2014 after donating a Kidney for my mom. The
I Ate a Lot & Never Lifted a Weight: This Gym Changed My Life In 3 Months
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At Ferris360, our Instructors have developed a signature dynamic warmup to prepare our clients for our high
Getting Out of A Rut after a Long and Brutal Winter!
"I can't even tell you how happy Ferris360 makes me.
I was in such a rut after such


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I don't get my dh anything for father's day
- 40 Replies
He's not MY father.
am I crazy for feeling this way?
Quoting Anonymous: Everyone is different. I get my dh something. I love him and want him to feel special and loved in his day. He does the same for me on mothers day
I got mine a card from both me and DS and got him a guava jarritos. Lol
Did you get anything for mother's day?
I find father/mothers day a day to celebrate the important father/mother in YOUR life. So seeing as my husband is an awesome father yup I do celebrate fathers day. I bought him an ipad mini. My kids made him some crafts.
neither did I, we're just not big on Hallmark holidays in this house and no I didn't get anything on Mother's day before anyone asks.


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Bariew Backpackers Lodge & Homestay
Hostel | Bario, Kelabit Highlands, Sarawak, East Malaysia, Borneo. Bario, Malaysia 98050
3 out of 3 customers would recommend this property
Recent review (18/05/2011)
"Although he appears shy and quiet at first, the owner, Reddish, is extremely friendly and has a great sense of humor. He is very knowledgeable about the area's history and its people. We hired him to be our guide for a…"Read all Bariew Backpackers Lodge & Homestay reviews
- Air Conditioning
- Bar
- Self-catering Facilities
- Currency Exchange
- Free city tour
- Games room
- Secure lockers
- Lounge area
- Internet / Wi-Fi
- Nightclub
- 24 hour reception
- Restaurant
- Safety deposit
- Tours/Travel Desk
- Washing machines
Purchasable Extras
- Airport Pickup USD 2.36
- Breakfast USD 1.89
- Linen USD 2.36
- Towel USD 1.18
What's Included
- Luggage Room
Cancellation Policy
Customers can cancel their reservation free of charge up to 2 days before arrival (local time). Payments made to HostelBookers are non-refundable.
Group booking?
Group of 15 or more traveling to Bario?Contact us directly
Enter dates to check prices
|Private Rooms A private room is a room that is not shared with other people from the property – in other words, you book and pay for all the beds in the room. If you are traveling alone, for example, but want to book a double room, you must pay for two people in order to book the whole room.
Private Triple
Private (5 beds)
Private (4 beds)
Private Double
Family Room (1 Single & 1 Double)
About Bariew Backpackers Lodge & Homestay
On arrival at the Bario airport, ask for Julfiness and Reddish who also drives the local taxi. A 10 minute ride from the airport will find you at Bariew Backpacker Lodge and Homestay.
The lodge is safe, clean, friendly operators, centrally located. Situated 2km from airport and within walking distance to shops, longhouses, rice fields, police post, immigration post, army post, clinic, internet/telephone, schools, games field and to some attraction sites i.e. grave yard, army airplane wreckage etc.
The Lodge includes 2 lounge rooms, single/double beds, kitchen, showers and western toilets, laundry, left luggage storage, localised nature guides, reading corner, rattan made furniture's, airport pick up, coffee/tea making facilities, etc.
the Lodge has it's own 4 X 4WD vehicles which will take you to the start of each walk or to other lodges and longhouses.
Bario is a base for jungle treks. Bariew Backpacker Lodge can arrange many activities such as overnight jungle trekking, camping, bird watching, sports fishing, sight-seeing, longhouse-visits, longboat trips, traditional farming experience and cultural entertainment. Jungle trekking from overnight to 5-6 days can be organized accommodating your needs and fitness level.
All meals are served in Kelabit style with the worlds most famous Bario Rice and pineapple curry.
Detailed list of activities, prices and area map of Bario Highlands is available at the lodge.
Enquiries most welcome.
warmest regards,
Your host,
Reddish and Julfiness
Other languages
- Deutsch: Bariew Backpackers Lodge & Homestay
- Español: Bariew Backpackers Lodge & Homestay
- Français: Bariew Backpackers Lodge & Homestay
- Italiano: Bariew Backpackers Lodge & Homestay
- Português: Bariew Backpackers Lodge & Homestay


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If so, what have you found that is share worthy? If not, why the hell haven't you? We should do a community share day where we all share a thread to let the world know our awesomeness.
kappus 0002/24/2013 4:27:33 PM PermalinkNot yet, but your thread has kindly reminded me that I should.
dazarobbo2/24/2013 4:03:36 AM PermalinkIt was frustrating me every time I accidentally rolled over it, so I killed it with AdBlock.
Viltre2/24/2013 4:07:42 AM PermalinkDon't have it. Probably because I keep all that stuff blocked through RequestPolicy and NoScript.
CrazzySnipe552/24/2013 12:34:46 AM PermalinkNerp. Haven't found something I felt like leaving a mark on my friends' Facebook feeds for.
M94 Mushroom Man2/23/2013 9:39:02 PM PermalinkI don't have a Facebook or Twitter, or anything of the like. Plus, I haven't found much that is share-worthy.
Recon Number 542/23/2013 9:21:14 PM PermalinkWhen the Bungie foundation visited that one 9 year old kid who was recovering from a liver transplant and gave him Noble 1's helmet? Oh HELL YES did I share that.


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Lansdale Borough Police know more about a missing Lansdale woman, Sergeant Alex Kromdyk says, Amanda Schumacker was seen in Philadelphia on North Broad Street Tuesday with a male.
"From that report, she was o-k, but we would really like her to reach out to Lansdale Borough Police to make sure she's safe."
Schumacker, 26, was reported missing after leaving the hospital on June 20th. She left behind her clothing, money and medicine, which caused additional concern
Police say, a Bridgeport man wanted in connection with the theft of thousands of dollars in wedding gifts has turned himself in. 35-year-old, Joseph Franzone Jr. turned himself in to the District Justice in Skippack Friday Morning. Police were able to use surveillance video to determine Franzon's identity in connection with the Sunday, July 7th theft of wedding gifts at the William Penn Inn. Police say, Franzone was seen roaming around in the parking lot and inside the William Penn Inn. Franzone made off with an estimated three to five thousand dollars in wedding gifts. Franzone faces a list of charges, including theft.
Burglars take advantage of the July 4th holiday to burglarize Inglewood Elementary school on Allentown Road. Towamencin Township Police Chief Tim Dickenson.
"They gained entry through an area that was under construction at the time. They took ten 20-foot long copper pipes, 18 brass ball valves, 100 copper fittings of various sizes and five reels of nearly 2,500 foot each of solid number 12 copper wire."
Dickenson says, the burglars hit the site between 3pm July 4th and 6am of July 5th. The copper has an estimated value of 12-hundred dollars. The value of the rest of the stolen property is still being assessed.
Police have identified the victim of a one-vehicle fatal crash in Lower Salford Township earlier this week. The dead man is 20-year-old Tyler Rizol of Harleysville. His car hit two trees before overturning on Schlosser Road. Rizol was pronounced dead at at the scene. He was a graduate of Lansdale Catholic High School.
Address: 1210 Snyder RoadLansdale, PA 19446
Phone: (215) 855-8211
Phone: (215) 723-2116


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Super Smash Bros. is, first and foremost, a console experience. From the N64 all the way to the Wii, the all-star Nintendo franchise has been exclusive to the company. This all changes with the next installment of the chaotic fighting series … well, installments, I should say, as the game is not only launching on the Nintendo Wii U, but the 3DS, as well.
This is the first time Smash will ever appear on a portable platform, yet my reaction was immediately sour. Sakurai's revelation that resources will be split to create two games only sounded like trouble to me – any resources not being devoted to the console game seemed like a tragic misstep. Would the Wii U version suffer from the 3DS's existence? That's the question that dominated my train of thought.


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Understanding the Effects of Works Councils on Organizational Performance. A Theoretical Model and Results from Initial Case Studies from the Netherlands**
Wigboldus, Jan Ekke, Looise, Jan Kees, Nijhof, André, Management Revue
In this article, we present a conceptual model to understand the effects of works councils on organizational performance. The model is based on economic and HRM literature on employee participation and organizational performance, as well as on German and Dutch research into the economic and other effects of works councils. The model has been operationalized through a research approach and applied for the first time using in-depth case studies in a large insurance company in the Netherlands. In this paper we present the results of these case studies and the consequences for the model and the research approach.
The findings show that works councils not only affect performance through changing employee attitudes and behavior - in a not dissimilar way to HRM - but also in a direct way by adding asymmetric information to the decision-making process and in an indirect way through influencing management attitudes and behavior. Thus the works council - performance relationship differs substantially from the HRM - performance relationship. The influence of the three potential mechanisms will depend to an extent on the characteristics of the works council and the organizational context. Further research is needed to determine how contextual conditions influence the positive effects of works councils on organizational performance.
Key words: works councils, organizational performance, the Netherlands
1. Introduction
The impact of HRM on organizational performance has become a popular field for research over the past few decades (Guest 1997: 263; Delery/Shaw 2001; Boselie/Paauwe 2002). Conceptual models of their relationship can be visualized as an interrelated series of boxes, starting with company strategy and HR strategies and then connecting these through HR practices and employee outcomes with different interpretations of organizational performance: operational, financial, or societal. Employee participation - recognized as a relevant HR practice - has been given little attention in this research and, where present, in most cases the focus is on direct or financial participation (Summers/Hyman 2005; Perotin/Robinson 2002; Kaarsemaker 2006). Representative forms of employee participation are virtually absent. While the performance perspective is an increasingly accepted view of HRM, works councils - an important and sometimes dominant instrument for representative participation in most EU member states (Rogers/Streeck 1995: 11) - are traditionally not associated with economic effects and some even consider it immoral to make a study of the economic effects of works councils.
However, there are good reasons to discuss the economic effects of works councils, including in the Netherlands. First, despite a tradition of over fifty years, some 2530% of enterprises have yet to establish a works council despite an obligation to do so. This level of default has been steady for many years and is hardly falling (Engelen/ Kemper 2006). It would seem that works councils are not seen as potentially making a contribution to organizational performance in those enterprises. Second, works councils have become an institution over the years and, while contributing to stable industrial relations, institutions tend to develop enormous inertia. How vital and relevant are works councils to firm performance? Is management really interested in the contribution of works councils or are they sidelined to dealing only with harmless issues? Perhaps works councils have a positive effect on firm performance without anyone reading it? Third, the economy is globalizing fast and, as part of an open economy, Dutch enterprises are heavily influenced by international business. This also affects industrial relations. Although national legislation and traditions will not be erased overnight, the Dutch system of workers' participation will be put to the test by managements from other cultures such as the US, Great Britain and Japan. We need better arguments to defend the perceived advantages of our industrial relations system than the phrases that have proved adequate within the country. …


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The Crowne Plaza® Brand Brings PGA Tour Pro Rickie Fowler's Tips on Winning to an Inbox Near You
New Interactive Video Campaign Featuring PGA TOUR Pro Supports "The Big Win" Promotion From IHG® Rewards Club
ATLANTA – The modern day business traveler likes to win, both at work and when they're having fun. To make winning easier, the Crowne Plaza® Hotels & Resorts brand has teamed up with PGA TOUR pro Rickie Fowler to share secrets for winning on and off the course. The upscale hotel brand and Fowler, one of the most recognizable golfers on the PGA TOUR, have crafted a series of interactive videos to tee up the latest round of "The Big Win" promotion from its hotel rewards program, IHG® Rewards Club.
The light-hearted videos, released today, feature tips from Fowler on how to make the next business trip – and round of golf – a success. Using proprietary innovative technology from cloud-based platform Stargreetz that delivers personalized content, the videos begin with Fowler greeting IHG® Rewards Club members by name, providing tips on winning at golf, while highlighting offers from "The Big Win," a first of its kind promotion that provides each member with an individually tailored set of offers.
"For our Crowne Plaza guests, traveling for work is a necessary part of their career, and they feel like they're winning at travel when they get rewarded for staying at our hotels," said Gina LaBarre, vice president, Americas brand management, Upscale Brands, IHG. "This video campaign is a fun way to highlight how we help them win on the road, but we also share a few humorous golf tips from Rickie Fowler. In the spirit of 'The Big Win' promotion, we've used innovative technology to personalize the videos from Rickie, just like this promotion personalizes offers for our guests."
In addition to the personalized member videos, the campaign features a shareable element designed to spark interaction among consumers. Rather than helping their friends improve their golf game, IHG® Rewards Club members can "prank" their golf buddies by sending fake tips from Fowler designed to mess up their game. This marks the brand's third consecutive golf season engaging Fowler, who at just 25 has been dubbed the "next generation of golf."
"I'm not the typical golfer, and I enjoyed filming something that's not a typical hotel advertisement," said Fowler. "It's nice to poke a little fun at golf sometimes, like pretending that closing your eyes when putting will help you get closer to the hole. Hopefully some of my buddies will take this advice."
Created with PGA TOUR Entertainment and Ogilvy and shot at Riviera Country Club in Southern California, the videos will be progressively rolled out online starting today and will extend through the end of "The Big Win" promotion on April 30. Current IHG® Rewards Club members and new members can register for "The Big Win" promotion here.
For behind the scenes videos of Fowler on set, visit the Crowne Plaza YouTube page.
IHG, the parent company of the Crowne Plaza brand, is the Official Hotel and Resort Partner of the PGA TOUR, Champions Tour and Web.com Tour, and the Crowne Plaza brand is the title sponsor of the Crowne Plaza Invitational at Colonial. The 2014 Crowne Plaza Invitational at Colonial will be held May 19-25 at Colonial Country Club in Fort Worth, Texas, and Fowler has committed to playing in the 2014 tournament. The Crowne Plaza Invitational at Colonial is the longest running PGA TOUR tournament still held at the original site.
About Crowne Plaza® Hotels & Resorts
Part of the IHG global portfolio, the Crowne Plaza® Hotels & Resorts brand is a dynamic upscale hotel brand located in nearly 65 countries around the world in major urban centers, gateway cities and resort destinations. As the fourth largest upscale full-service hotel brand in the world and one of the fastest growing, the Crowne Plaza brand has a solid foundation for future growth. The portfolio has doubled since 2003 to nearly 400 hotels, and the global pipeline has increased fourfold to nearly 100 hotels. At Crowne Plaza hotels, we help our guests feel energized and productive, with 24-hour business services, wireless Internet access in the lobby, and fitness and leisure facilities. Our Sleep Advantage® program ensures our guests get a great night's sleep to power through their day. For reservations at Crowne Plaza properties, visit www.crowneplaza.com or call 1-800-2CROWNE. Connect with us on Twitter: or Facebook: .
About IHG® Rewards Club
With more than 77 million members globally, IHG® Rewards Club, formerly Priority Club® Rewards, is the first and largest guest loyalty program in the hotel industry. Named Best Hotel Rewards Program in the World nine years running by Global Traveler magazine, IHG® Rewards Club offers sought-after benefits and is easy to use. In addition to unique features like No Points Expiration, No Blackout Dates on Reward Nights, Points & Cash, Flights Anywhere™ and Hotels Anywhere, IHG® Rewards Club members have hundreds of options for point redemption including: Reward Nights, music downloads, brand merchandise and retail gift cards. Enrollment in IHG® Rewards Club is free. Guests can sign up at ihgrewardsclub.com, by calling 1-888-211-9874 or by inquiring at the front desk of any of IHG's 4,700+ hotels worldwide.
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IHG manages IHG® Rewards Club, the world's first and largest hotel loyalty program with over 77 million members worldwide. The program was relaunched in July 2013, offering enhanced benefits for members including free internet across all hotels, globally.
IHG franchises, leases, manages or owns over 4,700 hotels and 687,000 guest rooms in nearly 100 countries and territories. With more than 1,100 hotels in its development pipeline, IHG expects to recruit around 90,000 people into additional roles across its estate over the next few years.
InterContinental Hotels Group PLC is the Group's holding company and is incorporated in Great Britain and registered in England and Wales.
Visit www.ihg.com for hotel information and reservations and www.ihgrewardsclub.com for more on IHG Rewards Club. For our latest news, visit: www.ihg.com/media, , or www.youtube.com/ihgplc.
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I enrolled in First Amendment law this semester, more because I felt I shouldn't graduate law school without taking it than because I had a real passion for the subject material. It turns out that the decision is, quite possibly, the best course selection I made all year. As seems appropriate for a class on […]
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It all comes from the same account and my friends, she has ways of finding out, and will bring it up at a time when you least expect it to happen. I just heard about the lens I thought she knew nothing about. It is best just to tell her you did it, and it can't be returned, and then slink out to the couch for a couple of days leaving for work before she gets up and staying at work but calling home until she has gone to bed.
I am off to the couch


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- Overall Length:8.28"
- Blade Length:3.44"
- Cutting Edge:3.08"
- Blade Thickness:0.14"
- Blade Material:CPM-S35VN
- Blade Style:Clip Point
- Blade Grind:Flat
- Finish:Satin
- Edge Type:Plain
- Handle Length:4.81"
- Handle Thickness:0.46"
- Handle Material:G-10
- Color:Brown
- Frame/Liner:Stainless Steel
- Weight:3.75 oz.
- User:Right Hand, Left Hand
- Pocket Clip:Tip-Up, Tip-Down
- Knife Type:Manual
- Opener:Thumb Hole
- Lock Type:Compression Lock
- Brand:Spyderco
- Model:Paramilitary 2
- Model Number:C81GPBN2
- Country of Origin:USA
- Best Use:Tactical
- Product Type:Knife
Be sure to check out our other Spyderco Paramilitary 2 knives!
Spyderco Limited Run Para-Military 2 in earth brown G10 and S35-VN!
This next generation Para-Military knife features a number of inspiring revisions. The revised handle has a thinner profile at the end to improve the ergonomics and the ambidextrous four-way reversible pocket clip rests deeper in the pocket as well. The blade has an elongated profile, with an increased cutting edge, finger choil and thumb ramp. Implementation of a new Bushing Pivot System generates fluid action and more consistent manufacturing tolerances. The Para-Military still has the solid and confidence inspiring Compression Lock on the handle spine. Lastly, the size of the lanyard hole has been generously increased, giving the use more carry options. This all results in more than just change for the sake of change, it's a real improvement on an already great knife.
This Para-Military knife has a premium full flat ground S35VN blade with a plain edge. The handle scales are earth brown G10 for impressive strength and grip. Sprint-Run, limited quantities.


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At River Dance Lodge, Idaho's Outdoor Adventure Resort, our handcrafted log cabins are comfortable retreats with hot tubs and gas stoves on the Clearwater River. Vacation with your family, plan your destination wedding or retreat with a group.
Nestled in the Idaho Mountains, you'll find the Ashley Inn is a perfect place to rest, relax at an affordable rate. On our website you will find all the activities in our area. Your own fireplace & robes, breakfast included, Warm cookies every night
Super 8 Moscow is adjacent to University of Idaho and approximately 75 miles from Spokane Airport.
This recently remodeled property is located in Moscow, ID in the heart of the Palouse Region.
The hotel is one of the premier hotels in Moscow, Idaho and is adjacent to the University of Idaho and only eight miles away from Washington State University
The La Quinta Inn Moscow Pullman is situated approximately five miles from Pullman-Moscow Regional Airport and 36 miles from Lewiston-Nez Perce County
The Golden Oak Inn Lewiston, a Magnuson Hotels affiliate, is located one mile east of downtown Lewiston, Idaho on the North and South Highway.
The Econo Lodge Lewiston hotel is centrally located close to area attractions like Lewis-Clark State College, Hells Gate State Park, the Snake River, Hells
Located in Downtown District with a wonderful flare of Historic Tasteful Lewiston.
Unexpected quality, unexpected service, unexpected extras No other budget hotel comes close to the value you will find at the Inn America A Budget Motel.
Find great deals on quality hotels & resorts in Moscow Idaho, or indulge at one of our luxury resorts. Take advantage of the many amenities that can be included with your stay such as in-room internet access, wifi, room service, fitness center, pool, hot tub, steam room, and shuttle services to and from the airport. Browse our hotels and resorts in Moscow Idaho and plan your Moscow Idaho vacation today!


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Sgt. Ashley Ruple helps Alexis Tischer, 6, shop for a Justin Bieber shirt on Thursday afternoon at the Petal Walmart as part of the Petal Police Department's Shop with a Cop program.
- Filed Under
Two towheaded children pranced around Petal's Walmart on Thursday with eyes widened in Christmas cheer as they shopped for clothing and toys alongside two officers of the law.
Nathan Tischer, 9, and Alexis Tischer, 6, were two of 13 children benefiting from Petal's annual Shop with a Cop program.
Angela Hoeflich, administrative assistant to the chief of police, said fundraisers and donations allowed the program to help four families this year. ...


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Beecroft: What the ECB should do, and probably won'tOwen Thomas
Despite worryingly low inflation, an uncomfortably high euro dollar rate and feeble growth, the ECB, which meets on Thursday, probably won't bow to the sort of pressures to which other central bank would yield. So says the Chairman of Saxo Capital Markets, Nick Beecroft. He says it's less than 50-50 they'll do anything, certainly this month.
The problem, says Nick, is that alternative forms of easing entail some drawbacks, either politically or in terms of their efficacy. While Monday's low 0.5% year-on-year CPI reading must surely have increased the chances of some stimulus, the ECB may easily choose to wait and see what happens in the coming months, both in the States and in Europe, and are likely just to label any surprising data points as transitory.


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Protecting the First State
Celebrating 25th of working for Delaware's nature and people. Help us to ensure a future for Delaware!
Every month, the Conservancy's free e-newsletter brings you updates from our conservation work around the world — plus incredible nature photos, green living tips you can use and invitations to events near you!
Help us ensure the diversity of life on Earth by joining our global community online — it's simple and free! Check out some past issues of Great Places and then sign up today!
Latest News & Features
2015 Highlights
The Nature Conservancy's 25th anniversary year in Delaware saw big progress on our major initiatives.
Winning Big for Deep-Sea Corals
The Nature Conservancy played a major role in a new, historically significant protection for a sensitive deep water environment.
25 Great Years and Counting
From a little over an acre to more than 30,000, the Delaware chapter has helped protect some of the state's most precious natural resources.
Latest Photos
LEAF Slideshow
See what our interns have been up to this summer!
Shortleaf Pine Restoration
See what we're bringing back at our Pemberton Forest Nature Preserve's Ponders Tract.
Horseshoe Crabs
Watch a slideshow and see what volunteers witnessed at Big Stone Beach while measuring the abundance and health of horseshoe crabs, which have visited our shores since prehistoric times.
Latest Videos
Dogfish Dash
The 2013 Dogfish Dash raised $51,600 for The Nature Conservancy's work in Delaware.
Shorebird Miogration
Check out a video about the annual shorebird migration that occurs each May and June in conjunction with horseshoe crab spawning. It's a natural phenomenon that shouldn't be missed!
Annual Horseshoe Crab Count
Volunteers count crabs during May and June every year.
Recent Press
- August 25, 2015
- State Director Richie Jones Featured in Two Local Magazines
- June 09, 2015
- Pamela Sapko Joins The Nature Conservancy, Delaware Chapter, As Director of Operations
- May 15, 2015
- The Nature Conservancy, Delaware Chapter, Trustees and Staff Make Annual Horseshoe Crab Survey a Family Affair


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Pittsburgh, PA (Sports Network) - Jesus Montero hit a go-ahead solo home run in the seventh inning and Felix Hernandez tossed eight stellar innings to help the Seattle Mariners down the Pittsburgh Pirates, 2-1, in the finale a two-game interleague set at PNC Park.
Montero launched a one-out homer off of Pittsburgh starter A.J. Burnett (3-3), accounting for one of just two hits allowed by the right-hander, to snap a 1-1 tie. Hernandez (5-2), meanwhile, won his fourth consecutive start after allowing one run on six hits and striking out five.
Michael Saunders walked three times and scored the other run for Seattle, which has won four of six overall.
"It was big for us," Hernandez said. "We're coming from where we lost in Toronto the last game there and the first game here. We just wanted to win this one so bad."
Starling Marte had two hits and scored Pittsburgh's only run on a first-inning single by Andrew McCutchen.
Burnett struck out nine batters and allowed two runs over seven innings, though he walked four and had two wild pitches for the Pirates, who have lost three of four.
With the score deadlocked at 1-1 through six innings and Burnett's only allowed hit coming off of Endy Chavez' infield single in the fifth, Montero changed the game with one swing.
He smacked his solo shot over the right-center wall on Burnett's 100th pitch of the game to give the visitors a 2-1 advantage.
"I was looking for a fastball and he left it in the middle," Montero said, "and I hit it good."
Tom Wilhelmsen cruised through the ninth to earn his ninth save of the season.
"He never hit that other gear with his velocity -- but the spin and sequences were there," Pirates manager Clint Hurdle said of Hernandez.
Burnett retired the first nine batters he faced before running into some trouble in the fourth by walking two batters and throwing both of his wild pitches.
Saunders drew a leadoff walk and advanced to second on the first wild pitch of the inning. Jason Bay then walked, but Burnett struck out Kyle Seager and forced a groundout to get two quick outs. Saunders, though, scored from third on Burnett's second wild pitch of the frame to tie the game at 1-1.
The Pirates jumped on the board early in the first inning as Marte smacked a leadoff double down the left-field line. After Travis Snider struck out, McCutchen hit a single up the middle to give the hosts a 1-0 lead.
Hernandez has allowed two earned runs and struck out 28 over 30 innings in
his last four starts ... Pittsburgh recalled pitcher Duke Welker from Triple
A Indianapolis Wednesday and released pitcher Jonathan Sanchez ...
Hernandez is 14-7 in 27 interleague starts over his career ... Burnett has
not allowed more than three runs in any of his eight starts this season.
Pope Francis' visit to Philadelphia in September has altered the date of the Penn-Villanova football game.
Kyle Busch won Sunday's road course race in Sonoma, California, but he still has a long way to go to become eligible for a spot in the Chase for the Sprint Cup championship.
NFL players have a very short shelf life to make significant amounts of money so it's tough to fault anyone who has outperformed a contract when they go knocking on the door to ask for more.
Pete Mackanin will remain interim manager of the Philadelphia Phillies for the remainder of the season, the team announced Tuesday.
The Oklahoma City Thunder have reportedly retained restricted free agent forward Kyle Singler with a five-year contract extension.
The Detroit Red Wings signed veteran forward Brad Richards a one-year contract on Wednesday.


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Personal Campaign
Wai Ling is gathering 10 signatures to
Tell The Usda To Shut Down Law Breaking Puppy Mills!
It was because puppy mills bring all dogs pain , and cruelty for puppy alive until puppy death , the puppy mills no good health and welfare for dogs , this is wrong , must shut down all puppy mills . this must respect and love dogs , give real health and good welfare for their alive , make them happy and no pain for them , thank you


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3 to 6 days
Free delivery when you spend over £75 (GBP).
Will be calculated at checkout
Party blouses - Patterned
You must select a size
Top by TIGER OF SWEDEN. Transparent style with a round neckline. Shorter section in the front.
Made of 100% Polyester.
Tiger of Sweden Jeans is a strong competitor in the denim market. Innovation, quality and pride are some of the key words. Tiger of Sweden makes Scandinavian fashion with a clean and young design. Find Tiger of Sweden fashion for women and men here at Nelly.com UK.
Free delivery when you spend over £75. Standard shipping £3.95. Delivery time: 3-6 days. We deliver with UPS.
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Recent Headlines
Today in entertainment history: Nov. 30
A look at the Hollywood headlines of years past.
in Music
TOP 10: Count down this week's hottest tracks
The top 10 songs from the Billboard Hot 100 chart for the week ending Nov. 28.
Anne Hathaway expecting first child
The Oscar winner is expecting her first child, according to reports.
OPENING WEEKEND: Family-friendly 'The Good Dinosaur,' boxing drama 'Creed' among Thanksgiving releases
Here's a look at some of the films set to open this weekend.
in Music
Adele announces tour
Adele, whose latest album "25" has smashed sales records in its first week of release, announced on Thursday she would begin a 15-week concert tour.


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It is time for the Seventh-Annual Catchy Awards to honor the top student athletes of the year. The media and coaches have spoken with their all-state, all-league and all-area teams. This is a chance for you, the fan and supporter of high…
Time and time again this year, the Wichita Collegiate track and field team has proven that you don't need to have large numbers to perform well, just talent.
State Basketball began on Wednesday with quarterfinal play, while state champions will be named on Saturday, March 15. Below are the brackets for every State Tournament. (Links available on desktop only. Please check the Catch It Kansas…
Unlike the boys first round a day before, the 3A Girls State Tournament gave fans three opening round upsets, including the bracket's top seed, Riley County falling to No. 8 Lyons.
After losing two straight Sub-State Championship games to Holcomb, the Cimarron Lady Bluejays had to begin wondering if winning the tournament just wasn't in the cards.
With four of the top 10 ranked teams in girls Class 3A all in one sub-state bracket, there was bound to be some unhappy faces after Friday night's semifinals.
It's Friday night during Sub-State week and whether your team was in the Semifinals or the Championship the pressure of win or go home is imminent. Read on to see how your team performed.
Boys tournament week may have brought big changes to the rankings, but there was little change at the top of the rankings after girls tournament week.
Tournament week on the boys' side brought with it some big shake-ups in several classes of this week's KBCA Basketball polls.
While the number of school and teams may change from year-to-year, the Hi-Plains League Tournament has been a constant mid-season measuring mark for Southwest Kansas area teams since 1964.
Boys tournament week has arrived and the Catch-All will update you on scores from around the state.
The January 19th basketball rankings have been compiled and released. In 6A, the Maize girls dropped from second to third in the rankings while Wichita East boys moved up from third to second.
The KBCA has released rankings for the week of January 12. The Hesston Swathers look like a force to be reckoned with, as both boys and girls are top ranked in 3A.
Each year, Dodge City tries to schedule one basketball doubleheader at the United Wireless Arena to give the kids a chance to play under the bright lights of the city's state of the art arena. This year's match-up was billed the "Backyard…
Only seven of them left before postseason begins, but another Friday night is in the books for the girls' basketball teams around the state.
The first full Friday of 2014 has come and gone and we have all the scores and stats from Boys Basketball.
The first full week of basketball of 2014 is here. See how your team kicked off the year in the Girls Basketball Catch-All.
The new year is here and teams are ranked according to their performances from 2013. The brand new rankings were released and are the first of 2014.
Tonight marked the final night of high school basketball in 2013. This was the chance to win one last time before the kick off of 2014, but only half the teams in the state will go into the break with a win.
After 4 days and 24 games, the Bluejay Invitational in Cimarron came to a close late Saturday night and with a bang.
The Kansas Basketball Coaches Association has released the first rankings for the 2013-14 season.
The Hi-Plains League announced it's first and second team honors today. Cimarron holds five spots while Meade holds five.
Cheney High's Brooklyn Lonker shared an individual Class 3-1A state golf title to highlight the Cardinals' first state championship.
The first six weeks of the football season are important, but the real work begins in Week 7.
Post season is already here, and athletes are out and about competing for a spot at state.
Longtime area football coach Roger Robben has passed away.
Addison Root of Collegiate Wichita signed with Memphis University this morning. Root is the second volleyball player from Collegiate to sign with a Divison 1 school.
Four classifications full of Golf state tournaments are in progress today across the state. Check back for results from Class 6A, 5A, 4A, and 3-2-1A.
Derby's Tiffany Voss signed to the University of Sioux Falls for tennis. She said USF features a solid tennis program and great academics. She had other offers from Concordia University and Nebraska.
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DTS, Inc. to Exhibit at 2013 International CES, booth Central Hall, Booth 9839, Jan. 8 - 11, 2013, in Las Vegas NV, US 2013 International CES 2013 International CES:DTS, Inc. Business Wire CALABASAS, Calif. -- January 10, 2013 Press Kit Materials are Available at: http://www.tradeshownews.com/events/2013-International-CES/DTS/ Company: DTS, Inc. Booth/Stand: Central Hall, Booth 9839 2013 International CES Event: Jan 8 - 11, 2013 Las Vegas, NV, US Web: http://www.dts.com/ Facebook: http://www.facebook.com/DTS.Inc Twitter: http://twitter.com/DTS_Inc About DTS, Inc. DTS, Inc. (NASDAQ:DTSI) is dedicated to making digital entertainment exciting, engaging and effortless by providing state-of-the-art audio technology to more than a billion consumer electronics products worldwide. From a renowned legacy as a pioneer in high definition multi-channel audio, DTS became a mandatory audio format in the Blu-ray Disc™ standard and is now increasingly deployed in enabling digital delivery of compelling movies, music, games and other forms of digital entertainment to a growing array of network-connected consumer devices. With the acquisition of SRS Labs, DTS strengthens its position as a leader in providing end-to-end audio solutions to the rapidly growing network entertainment sector. DTS technology is in car audio systems, digital media players, DVD players, game consoles, home theaters, PCs, set-top boxes, smart phones, surround music content and every device capable of playing Blu-ray™ discs. Founded in 1993, DTS' corporate headquarters are located in Calabasas, California with its licensing operations headquartered in Limerick, Ireland. DTS also has offices in Los Gatos and Santa Ana, California, Washington, China, France, Hong Kong, Japan, Singapore, South Korea, Taiwan and the United Kingdom. For further information, please visit www.dts.com. DTS, the Symbol, and DTS and the Symbol together, are registered trademarks of DTS, Inc. All other trademarks are the properties of their respective owners. © 2013 DTS, Inc. All rights reserved. Contact: DTS, Inc. PR Contact Michael Farino, 949-442-5404 firstname.lastname@example.com or PR Contact Melody Demel, 619-234-0345 email@example.com
DTS, Inc. to Exhibit at 2013 International CES, booth Central Hall, Booth 9839, Jan. 8 - 11, 2013, in Las Vegas NV, US
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Home | About | Small Groups | Events | Team | Good to know
Founded in Zurich in 2009, The Powerhouse is a volunteer-run, internationally-focused network for professional women – whether currently in the work force or not.
Click HERE to join our mailing list.
Upcoming event
Women and Wellbeing
When women thrive, businesses thrive
Friday November 6th, 7pm
The Powerhouse is delighted to invite you to our next event to hear Aniela Unguresan speak about Women and Wellbeing – a critical issue for the business world today, and in the future.
More info on our Events Page
NEW regular event:
Area 2 Small Group – Zürich city
8:30 to 10:00 every first Thursday of the month
This group aims at tackling entrepreneurial related topics where you can not only socialise, network, but also have or give input on how to do things in a different way and brainstorm together to find solutions to entrepreneurial issues.
For more details, please contact Madalina or Mirkka directly
area2 [at] powerhousecollective [dot] com
or RSVP within the Powerhouse FB Group, section Events
Thank you ...
to everyone who made it along to our interactive evening with Ashley Ringger and Rebecca Wheatley, focusing on human-centered communication, grounded in representing your authentic character/brand.
Photos on our Facebook page.


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Lijiang Old Town Stock PhotoAncient,Architecture,Bridge - Man Made Structure,Building Exterior,Built Structure,Bush,Chinese Culture,Chinese Lantern,Color Image,Connection,Day,Flower Pot,Horizontal,Incidental People,Lijiang,Naxi,Old Town,Outdoors,Photography,River,Sky,Travel Destinations,Tree,Yunnan ProvincePhotographer MelindaChanCollection: Moment MelindaChanOld Town of Lijiang, well-preserved old city of ethnic minorities with brilliant culture, is central town of Lijiang Autonomous County of Naxi Ethnic Minority in Yunnan Province.


text: In Ostargar, there is a mission called Tainted Blood that takes place right before you become a Grey Warden. Simply collect the three Vials of Darkspawn Blood but NOT the Grey Warden Treaties. Return to camp and talk to Duncan and report "We have the blood, but not the scrolls." This will exit out of the conversation, but WILL give you the experience for the mission. Rinse and repeat until you are at the desired level. It takes very little time to shoot up to lvl 25(cap) if you press X to skip the conversations and speed up the process.


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United States Department of Agriculture
Agricultural Research Service
1a.Objectives (from AD-416):
Objective 1: Analyze mRNA and storage oil profiles of cotyledons over the course of seed maturation to infer biological networks underlying soybean seed oil composition and content, and to predict their key gene candidates.
Objective 2: Analyze small RNA profiles of cotyledons over course of the seed maturation, and further predict small RNA candidates regulating soybean seed oil composition and content.
1b.Approach (from AD-416):
Objective 1: Soybean oil synthesis and deposition occur mainly in cotyledons and are differentially regulated over the course of seed maturation. The oil synthesis and deposition are accomplished through the concerted activities of many gene products and biological pathways that are primarily regulated at transcription levels. Gene expression patterns change very quickly over the course of an organism's evolution if it is not subjected to functional constraints. Although it is not a universal rule, evidence suggests that expression patterns of many genes are intended to co-evolve with their biological functionalities. The co-evolution is reflected by a correlation of gene expression pattern with the related biological functions, and co-expression of functionally related genes such as those that encode proteins that reside in the same metabolic or signal pathways, or in the same cellular complexes under a variety of biological conditions (Stuart et al., 2003; Wei et al., 2006). The expression pattern correlation is widely used as criteria to predict biological functions of genes, functional relatedness between genes, and gene regulatory networks (Horan et al., 2008). We have examined transcriptomes and storage lipid profiles of cotyledons at six distinct developmental stages over the course of seed maturation.
Objective 2: We have conducted deep sequencing of small RNA populations in the same RNA preparations used for transcriptome analysis. It is believed that miRNAs function as master regulators in gene regulatory networks underlying diverse biological processes in Arabidopsis. However, much less is known about soybean miRNA species and their accumulation patterns over the course of seed maturation. As part of our effort to delineate regulatory networks and identify key genetic components controlling oil composition and content, we will conduct genome-wide characterization of small RNAs, particularly miRNAs, in cotyledons over the course of seed maturation. We will use a bioinformatic approach to analyze the small RNA sequences we have conducted to discover miRNA species and their accumulation patterns. MiRNA species recognize their target mRNA by high sequence complementation, and function mainly as suppressors of the accumulation of the target mRNA by directing the degradation of its functional target mRNAs in plants. Sequence complementarities and negative correlation of mRNA and miRNA accumulation patterns should offer a more effective approach to identify the functional target genes, and can be used to delineate the topology of small RNAs in the TF networks inferred in Objective 1. The miRNA species that locate in TF networks enriched with oil related genes or target oil related genes would be strong candidates for future investigations for oil composition and content improvement.
3.Progress Report:
Production of storage oil in seeds requires concerted activities of many genes and biological pathways over the course of seed maturation. However, lack of knowledge on the intricate biological network and the availability of its key regulatory components has been a bottleneck in the effective application of both breeding and biotechnological approaches for soybean oil quality improvement. To address this problem, we determined profiles of message RNAs, small RNAs, DNA methylation, and major lipid species in soybean cotyledons (soybean cv. Jack) over the course of seed maturation. Having developed and applied a variety of bioinformatic strategies and tools, we identified the genes and biological pathways that are differentially regulated over the course of seed maturation, and inferred a set of transcription factor networks underlying soybean seed maturation. The regulatory networks potentially regulating oil quality traits were also identified. We identified and characterized in detail a set of miRNAs expressed in soybean cotyledons. The research provided great insight into molecular regulatory mechanisms underlying seed maturation and oil qualities at the gene and systems level. The project expired on 3/25/2013.
Illustration of miRNAs and their underlying regulatory networks in soybean cotyledons. Micro-ribonucleic acids (miRNAs), small RNA molecules that control which genes are expressed or repressed in plant tissues, are an emerging class of genetic control agents that regulate a wide range of biological processes. Of prime interest to soybean researchers is if these new genetic regulators (miRNAs) control the synthesis and storage of oils and proteins found in the soybean cotyledons, the main site within soybean seeds of these important commodities. However, little is known about what miRNAs accumulate in soybean cotyledons during the stages where these compounds are made and stored during seed development, and how they may regulate these processes. ARS scientists in St. Louis, MO sequenced 292 million small RNA molecules isolated from soybean cotyledons and determined that a total of 243 miRNA genes were expressed in these tissues. The cotyledon miRNAs were predicted to regulate 1061 genes. By applying a computational model, the regulatory networks within which these mRNAs control the expression of the target genes in cotyledons was inferred. This comprehensive characterization of miRNAs and their regulatory networks will open up new avenues and strategies for developing new soybean varieties that have improved oil and protein qualities that benefit both producers and consumers.


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Chase Tyler Band
Baton Rouge, LA
Thursday, March 27th, 2014 at 6:30pm
All Ages
Chase Tyler Band @ Superior Grill
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A year later, Duck Lake fire region in U.P. continues to rebuild
3:16 PM, Aug. 22, 2013
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A year later, Duck Lake fire region in U.P. continues to rebuild
Fragrant piles of yellow sawdust cover the floor around Bruce Marshall's gleaming new band saw, while freshly cut boards lie in neat stacks around the shed that shelters his one-man sawmill operation.
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