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Q. Does the Washington Law Against Discrimination include protections for people based on their sexual orientation or gender identity?A. Yes, the Washington Law Against Discrimination prohibits discrimination on the basis of sexual orientation and gender identity in the areas of employment, housing, public accommodation, credit, and insurance. That prohibition became part of the Washington State Law Against Discrimination (RCW 49.60) on June 8, 2006.
A. As defined in the law, "sexual orientation" means heterosexuality, homosexuality, bisexuality, and gender expression or identity.
A. As defined in the law, "gender expression or identity" means having or being perceived as having a gender identity, self-image, appearance, behavior, or expression, whether or not that gender identity, self-image, appearance, behavior, or expression is different from that traditionally associated with the sex assigned to that person at birth.
Q. What does gender expression or identity mean?
Q. If a covered entity (such as an employer) believes or assumes a person's sexual orientation is different from what it really is, and acts in a discriminatory manner based on that perception, is the person protected?
A. No. A Washington Appeals Court decision, Davis v. Fred's Appliance, Inc., 287 P.3d 51 (2012) determined that the definition of sexual orientation does not cover "perceived" sexual orientation. So, for example, if someone is not hired because an employer believes that they are gay, but they really are not gay, that person is not protected under the law.
Q. Does the law just protect gay people?
A. The law protects all people, because everyone has a sexual orientation and a gender identity. Although persons who are gay, lesbian, or transgendered experience discrimination most frequently, heterosexual individuals may also be discriminated against based on sexual orientation. For example, if a heterosexual person who is not hired for a job because the business believes a gay person will better fit customers' expectations for the position, then that person may have a claim of sexual orientation discrimination.
A. In an employment situation, if benefits or privileges of employment are given to heterosexual persons who are married (insurance coverage for a spouse, for example, or an invitation for the family to join a company function), then the employer may be required under non-discrimination law to provide the same benefit or privilege to a person who is married to a same sex partner.
Q. How does gay marriage affect the rights people have under RCW 49.60?


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You can get a FREE movie ticket for any Disney movie up to $12 when you enter 6 codes from select Kellogg's products with Monster's U codes.
Kellogg's products with Monster's U codes include Mini Wheats, Rice Krispies, Crunch Nut, Frosted Flakes, Corn Pops, Krave, and other Kellogg's cereals.
To enter you codes, you need to sign up for Kellogg's Family Rewards.
You can enter codes until 12/31/13.
You can also get 2 FREE Disney music downloads for 1 code or a Mike Pillowcase from Monsters U with 4 codes.
The movie ticket comes as e-movie cash and is good on any Disney release. Some of the Disney movie releases for this year include Monsters U, The Lone Ranger, Planes, Frozen, and Saving Mr Banks.
Not sure if your theater will accept e-movie cash? Check HERE.

subset: commoncrawl


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Daily NewsView All News
Leading index slips 0.7%
August 21 2008
The Conference Board's U.S. leading index of economic indicators fell 0.7% in July, and now stands at 101.2.
The six-month change in the index is -0.9% for an annual rate change of about -1.8%. That's up from the -3.4% annual rate at the end of the first quarter. However, The Conference Board said weaknesses among leading indicators continue to be widespread.


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PARIS: Two incredibly rare albino alligators have arrived at their new French home - a Paris aquarium - after being bought from a reptile collector in Florida.
Thursday's snappy new reptiles, who are just over one year old, are part of an elite group of no more than 30 such alligators in the world.
reply to post by snarky412
Surely a DNA test would have told you what bloody sex they are and I would want to know that before I made the purchase.
Seems strange. Was obviously expensive, freight costs alone would be stiff.
Wow, never seen albino alligators before. I hope they have a long and happy life in their new home.
Surely a DNA test would have told you what bloody sex they are and I would want to know that before I made the purchase.
In babies this size it is very difficult to determine the sex and also quite stressful for the animal to be going around inserting fingers in order to find their copulatory organs... thus being the reason why they prefer to wait. (plus the fact that the clitoris can be mistaken for the penis in juvenile animals).
Just putting this out there as I am a vet and have worked from time to time with crocodilians.
Just don't get the ends mixed up and stick your finger in the mouth


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Label images from wines tasted
tasting notes
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tasting note
Louis Jadot, Corton-Charlemagne Grand Cru 1988
Gorgeous. Medium straw/yellow colour. Wonderfully rich nose of flowers, brioche and baked apples. Medium-full bodied on the palate, with mouth-filling, soft, evolved fruity flavours, but braced by lime acidity and a slatey edge that keeps it keen and fresh. A glorious, long finish with ever-changing nuances of fruit/mineral flavours. Drinking beautifully but I'd guess it still has time on its side. Terrific stuff. (03/98)


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jGuru Forums
Posted By:
Posted On:
Friday, November 8, 2002 12:59 AM
Hi everybody,
I need to know how i can resize the JComponents inside a JFrame. Once the size of the JFrame is made bigger by pushing the maximun button, in the frames upper right corner. The frames size is made bigger, but the components remain at the preffered size givin to them.Is it any simple method one can use.
The components have a prefferedSize() wich matches the prefferedSize() of the JFrame..
Re: Resize components in a JFrame
Posted By:
Posted On:
Friday, November 8, 2002 11:08 AM
If you use a LayoutManager, when the frame is resized, the LayoutManager should take care of resizing and redisplaying the components within the Frame.
Hope that helps,


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Undefeated Five Strike Red & Black Snapback Hat
Item # 196556
Take An Additional 50% Off
Stash Points: 1,297
What is this?This is the number of points you get in The Zumiez Stash for purchasing this item. Stash points are redeemable for exclusive rewards only available to Zumiez Stash members. To redeem your points check out the rewards catalog on thestash.zumiez.com.
Stay up on your style game like a true street champ in the Undefeated Five Strike snapback hat in the black and red colorway.
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Random: sometimes I think about moving my "blogging" to Twitter & using my website for free webcomics + other content.
Even Google doesn't want me to use Google Reader anymore: they hid the Reader link in the drop-down today.
anyone fancy drawing some webcomics for free? hahahaha oh god I think it's time for (say it with me) #DoctorWhisky


text:
Tactical Ripstop Pant - Dickies
Dickies Tactical Ripstop pant, engineered to work as hard as the officers who...
Officer Jefferson "Jeff" Taylor, who was struck by lightning on Monday, May 23, while on tornado disaster response duty in Joplin, passed away at a hospital here on Friday. He was 31 years old. He worked for Riverside, a suburb on the north side of Kansas City.
"Jeff Taylor volunteered to assist with the Joplin disaster recovery," said Greg Mills, Chief of Police and Director of Public Safety for the City of Riverside. "He was a highly dedicated officer and a devoted public servant, and we will always remember his sacrifice."
Officer Taylor is the first officer in the history of Riverside Police Department to die in the line of duty, and the first emergency disaster responder to pass away as a result of the tornado in Joplin on May 22.
Source: KY3.com
Mo. Officer Struck by Lightning In Medically Induced Coma


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Renowned make-up artist Laura Geller's cosmetics range features her innovative baked formulas and easy-to-apply, wearable colours and collections, perfect for women of all ages and skin types.
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Form 976, Claim for Deficiency Dividends Deductions by a Personal Holding Company, Regulated Investment Company, or Real Estate Investment Trust
This form is used by personal holding companies, regulated investment companies, and real estate investment trusts to claim a deficiency dividend deduction under section 547 or 860.
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The Graduate Center offers more than thirty doctoral programs in the arts, humanities, social sciences, and natural sciences, including History.
Learn about the Graduate Center's core faculty,
including Distinguished Professor David Joselit (pictured),
on the Faculty Profiles page.
Through its extensive public programming, the Graduate Center contributes to the intellectual and cultural vitality of New York City.
The Graduate Center has announced its
Doctoral Student Parental Accommodation Policies.
Support for the Graduate Center ensures continued excellence in arts and
sciences graduate education, research, and public programming.
David Scott Kastan, series ed.
A Midsummer Night's Dream
(Barnes & Noble, Shakespeare Series, 2007)
Barnes & Noble's Shakespeare Series features newly edited texts prepared by leading scholars from America and Great Britain, in collaboration with one of the world's foremost Shakespeare authorities, David Scott Kastan of Columbia University. Together they have produced texts as faithful as possible to those that Shakespeare wrote, providing contemporary scholarship, contextualizing essays, better notes, and an annotated bibliography of titles for further reading. Midsummer Night's Dream, Shakespeare's hilarious though often unnerving story of desire, confusion, and magic has delighted audiences for four hundred years. The editor, Mario DiGangi, provides a set of notes rich in insight into the play's many literary and cultural points of reference. Mario DiGangi is a associate professor of English at Lehman College and the Graduate Center.
Purchase this book
Submitted on: JUL 30, 2007
Category: English | Faculty Books


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Yesterday's winner tells you to eat your vegetables.
Today's contestants are:
Gif 1:
Gif 2:
Yesterday's winner was beard slap.
From now on, the former winners will battle it out for the crown. Here are today's contestants:
Yesterday's winner was even more sunglasses.
Today's contestants are in the slap category:
Yesterday's winner was WWE surprise.
Today's contestants are in the sunglasses category:
Gif 2
Eat your Vegetables was the narrow winner of yesterday's battle.
Today's contestants are in the omg category:


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Cliché Society back from the brink
The Crawley Cliché Society has made a dramatic recovery, only months after it nearly calling it a day. The society has been at the heart of the community for ever and a day, however, due to dwindling footfall, the writing had been on the wall until new society secretary Robert Gordon took the reins.
Posted: Apr 1st, 2014
More from News In Brief


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A man's body was found Sunday afternoon about a mile offshore from the Haulover Marine Center in North Miami Beach.
The body was discovered floating in the water by the captain of a fishing boat.
"It turned out to be something a little bit more gruesome," Captain Chad told Local 10.
The nationality or identity of the man has not been determined, but the U.S. Coast Guard said they will be searching the waters for other people who may be in distress.
Investigators will try to determine approximately how long the man's body was in the water.


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Macrophages are the dominant phagocyte at sites of wound healing and inflammation, and the cellular and acellular debris encountered by macrophages can have profound effects on their inflammatory profile. Following interaction with apoptotic cells, macrophages are known to switch to an anti-inflammatory phenotype. Activated platelets, however, are also a major component of inflammatory lesions and have been proposed to be pro-inflammatory mediators. In the present study, we tested the hypothesis that macrophage interaction with activated platelets results in an inflammatory response that differs from the response following phagocytosis of apoptotic cells.
Human monocyte-derived macrophages (hMDMs) were co-incubated with autologous activated platelets (AAPs) and the platelet-macrophage interaction was examined by electron microscopy and flow cytometry. The cytokines TNF-α, IL-6, and IL-23 were also measured during LPS-activated hMDM co-incubation with AAPs, which was compared to co-incubation with apoptotic lymphocytes. Cytokine secretion was also compared to platelets pre-treated with the gluococorticoid dexamethasone.
Macrophages trapped and phagocytized AAPs utilizing a mechanism that was significantly inhibited by the scavenger receptor ligand fucoidan. LPS-induced macrophage secretion of TNF-α, IL-6, and IL-23 was inhibited by co-incubation with apoptotic cells, but enhanced by co-incubation with AAPs. The platelet-dependent enhancement of LPS-induced cytokines could be reversed by pre-loading the platelets with the glucocorticoid dexamethasone.
The interaction of human macrophages with autologous platelets results in scavenger-receptor-mediated platelet uptake and enhancement of LPS-induced cytokines. Therefore, the presence of activated platelets at sites of inflammation may exacerbate pro-inflammatory macrophage activation. The possibility of reversing macrophage activation with dexamethasone-loaded platelets is a promising therapeutic approach to treating unresolved inflammation.
A major function of macrophages is phagocytosis of cellular and acellular debris during inflammation and wound healing, and the activation response of macrophages following phagocytosis can be varied depending on the local extracellular environment [1-6]. The importance of phagocytosis in the resolution of inflammation is emphasized by pathological conditions involving impaired phagocytosis, which may manifest as persistent infections or chronic inflammatory lesions such as diabetic ulcers and atherosclerotic plaques [7-11].
During their differentiation from primary monocytes, macrophages acquire specialized receptors and machinery for recognizing and clearing both apoptotic and infected cells [12]. In clearing apoptotic cells, macrophages use receptors such as scavenger receptors and integrins that function independently or in cooperation with each other depending on the type of cell targeted for phagocytosis [13-19].
Platelets are anucleate cells which play an integral role in maintaining vascular integrity. Within their 8-10 day lifespan, platelets can become activated either in the circulation or during adherence at a site of injury, and during this process they become targeted for destruction by macrophages [20]. The process of platelet activation involves several changes to the cell surface, including expression of P-selectin and loss of membrane asymmetry [21-23]. These changes in the platelet membrane may provide molecular signals to macrophages that trigger phagocytosis, although the precise mechanism by which macrophages recognize and phagocytose activated platelets remains to be identified.
Cells that have become apoptotic as part of their normal life cycle are recognized and cleared by phagocytosis in a manner that usually inhibits pro-inflammatory responses [24-27]. Although circulating platelets can exert a pro-inflammatory effect on circulating monocytes [28,29], their effect on differentiated macrophages, particularly at sites of inflammation, is not clear. We show here that phagocytosis of autologous platelets results in an pro-inflammatory profile that is opposite to the macrophage response following phagocytosis of apoptotic cells. Importantly, the platelet-enhanced, pro-inflammatory response of macrophages can be inhibited when the platelets are loaded with the glucocorticoid dexamethasone. In addition to novel insight into the macrophage inflammatory profile that exists in several diseases, these results also provide evidence that platelet-macrophage interactions are an important therapeutic target for reducing inflammation.
Monocyte-derived Macrophages
Human monocytes were isolated and cultured using techniques similar to those previously described [30,31]. Briefly, blood from healthy human donors was collected into citrate and peripheral blood mononuclear cells (PBMCs) were isolated by using Lymphoprep (Accurate Chemical) according to the manufacturer's instructions. Monocytes were further isolated by plating the PBMCs on gelatin-coated tissue culture flasks for 45 min at 37°C followed by 10 washes with phosphate buffered saline (PBS) to remove non-adherent lymphocytes. Monocytes were then detached from the flasks by incubation in 10 mM EDTA for 2 min at 37°C. Monocytes (250,000 in 500 μl volume) were then plated in 24-well plates overnight in RPMI 1640 supplemented with 10% fetal bovine serum (FBS) and 10 ng/ml recombinant human GM-CSF (R&D Systems). Monocytes were plated on glass coverslips for scanning electron microscopy (SEM) analysis and plastic tissue-culture plates for TEM analysis and phagocytosis experiments. Media was changed on day 2 and day 5, and after 7 days of culture the MDMs were used for phagocytosis and cytokine assays.
Platelets were isolated from whole blood collected into acid-citrate-dextrose (ACD) from healthy human donors and centrifuged for 15 min at 500 × g to generate platelet-rich plasma (PRP). PRP was pelleted by centrifugation for 10 min at 800 × g and the platelet pellet subsequently washed 2 times in citrated saline (pH 6.8). A portion of the platelet samples were degranulated by incubating 1 ml of platelets (250,000/μl in citrated saline) with 10 μl of 10 μM calcium ionophore A21387 (Sigma) for 15 min with rocking at room temperature, followed by three centrifugational washes with citrated saline. For phagocytosis experiments, platelets were fluorescently labeled with Cell Tracker Green CMFDA (Invitrogen) as previously described [32]. After the final wash, and prior to use in phagocytosis assays, platelets were resuspended in warm serum-free RPMI for 15 min at 37°C.
For analysis of surface P-selectin and phosphatidylserine, platelets (250,000/μl) were first incubated in either citrated saline or serum-free RMPI media for 1 hr at 37°C. A portion of the platelets were activated by including thrombin (King Pharmaceuticals, 1 U/ml final concentration) in the incubation reaction. To detect surface expression of activation markers in each platelet treatment group, a 10 μl aliquot of platelets was stained with either FITC-anti-CD62P (Biolegend) or FITC-Annexin-V (Biolegend) for 30 min at room temperature, after which the cells were fixed and analyzed immediately by flow cytometry. Flow cytometry analysis was performed using a CyAn flow cytometer (Beckman Coulter) and the Summit analysis software.
Dexamethasone-loaded platelets were prepared by incubating 1 ml of platelets (250,000/ul) in citrated saline with 5 ul of dexamethasone (Sigma, 10 mM in DMSO) for 15 min on a rocker at room temperature. Platelets were then washed three times with citrated saline to remove unbound dexamethasone. For subsequent experiments, 5 × 106 dex-platelets were added to each well of macrophages in a 24-well plate.
To prepare apoptotic cells, PBMCs were isolated as above, and following monocyte-adherence to gelatin-coated flasks the non-adherent lymphocytes were collected. Cells were rendered apoptotic (Annexin-V positive) by UV-irradiation for 10 min followed by overnight incubation in RPMI + 10% FBS at 37°C + 5% CO2.
Phagocytosis Experiments
Thirty minutes prior to the start of each experiment, 7-day old MDMs were washed 3 times with PBS and incubated with 500 μl fresh RPMI media. In some experiments the media was supplemented with 10% autologous human serum. A 25 μl aliquot of fluorescently labeled platelets (250,000/μl) was added to each well of macrophages. Platelets and macrophages were co-incubated for 45 min.
For SEM analysis, the co-cultures were washed once with PBS and fixed in 2% paraformaldehyde and 0.5% glutaraldehyde. The samples were then processed as previously described [33] and examined using a Cambridge S200 scanning electron microscope at 20 kV. For TEM analysis, the co-cultures were washed 5 times with PBS and fixed in 2% paraformaldehyde, processed as previously described [34], and examined using a Leo EM 910 transmission electron microscope. For flow cytometric analysis, warm trypsin/EDTA was then added to the macrophages to remove adherent platelets (confirmed by microscopy) and cells were incubated 15 min at 37°C. Macrophages were then collected and fixed in 1% cold paraformaldehyde and analyzed using a CyAn flow cytometer (Beckman Coulter) and the Summit analysis software. Data is expressed as the percentage of FL1-positive macrophages in a given collection of 10,000 macrophages. Data shown represent the average of at least 3 independent experiments and for each experiment 10,000 macrophages were analyzed.
Latrunculin (Sigma, 1 μg/ml final concentration), used as a pan-phagocytosis inhibitor, was added to a portion of macrophage-containing wells 30 min prior to addition of platelets. Fucoidan (Sigma) was added to macrophages at a final concentration of 250 μg/ml 30 min prior to addition of platelets.
Cytokine Experiments
Each well of MDMs was washed 3 times with PBS and incubated with fresh RPMI + 10% autologous human serum. Activated, degranulated, or dexamethasone-loaded platelets (5 × 106) were added to each well in addition to LPS (100 ng/ml). Some samples also received dexamethasone alone at a final concentration of 1 μM. After 24 hrs, supernatants were collected, spun 10 min at 14,000 g, and frozen at -80°C. Cytokines were measured by ELISA using capture and detection antibodies (eBioscience) per the manufacturer's instructions. Cytokines were measured in duplicate and averaged. The amount of protein secreted was normalized to the amount secreted by macrophages treated with LPS alone. Each experiment was performed at least 3 times using 3 different MDM donors. In each experiment, the platelets added were from the same donor as the MDMs. Treatment groups were compared using an unpaired t-test.
Macrophage Phagocytosis of Autologous Platelets
To examine the interaction between human MDMs (hMDMs) and autologous platelets, we utilized an in vitro co-culture system consisting of 7-day old hMDMs to which we added freshly isolated autologous platelets. The use of autologous platelets excludes the possibility that platelet-macrophage interactions are the result of an immune response triggered by the recognition of platelets as 'foreign.' The hMDMs and platelets were first co-cultured in serum-free RPMI media and examined by SEM and TEM at various time points to visualize the interaction between these two different cell types. As shown in Figure 1A, we observed platelets interacting with hMDMs during the first hour of co-culture. Platelets near the macrophages became entrapped by a network of macrophage filopodia, and although the macrophages were firmly attached to the coverslip and did not migrate, they appeared to direct groups of filopodia in the direction of nearby platelets that had settled on the dish. Visualization of these cultures suggests that an interaction between human macrophages and autologous activated platelets (AAPs) occurs in vitro, and that it occurs in the absence of serum proteins.
Figure 1. Electron Microscopic Analysis of Platelet-Macrophage Interactions. (A) hMDMs were incubated with fresh AAPs for 15 min (left panel) or 45 min (right panel) before processing for SEM analysis. (B) TEM analysis of hMDMs after 1 hr co-incubation with platelets. Arrow indicates phagocytic vacuole at high magnification.
Platelet phagocytosis by the macrophages in our co-culture system was subsequently confirmed by TEM and flow cytometry. Macrophages that were co-incubated with AAPs for one hour developed vacuoles, many of which contained contents that were the same size and shape as platelets (Figure 1B). These phagocytic vacuoles did not appear in control macrophages cultured in the absence of platelets. We then quantified phagocytosis by flow cytometric analysis of macrophage fluorescence after co-incubation with fluorescently labeled platelets and removal of adherent platelets with trypsin. When freshly isolated platelets were incubated in serum-free RPMI media and added in excess to 7-day old macrophages, approximately 50% of the macrophages internalized at least one platelet within 45 min (Figure 2). As expected, pretreatment of the hMDMs with the actin inhibitor latrunculin almost completely blocked phagocytosis, confirming the role of actin polymerization that occurs in all cases of phagocytosis (Figure 2A). The amount of phagocytosis increased if the platelets were pre-stimulated with thrombin (Figure 2B), and was significantly inhibited in the presence of fucoidan, a known competitive inhibitor to Scavenger Receptors [35,36] (Figure 2C). The presence of 10% autologous human serum had no significant effect on phagocytosis, which excludes the possibility that the platelet-macrophage interaction requires a soluble serum-bound "bridging" molecule. Together these results suggest that phagocytosis of platelets correlates with platelet activation, and that macrophage phagocytosis of autologous platelets may be mediated, at least in part, by scavenger receptors.
Figure 2. Flow Cytometric Analysis of Platelet Phagocytosis. hMDMs were incubated with an excess of fluorescently labeled platelets for 45 min in RPMI media alone (white bars) or containing 10% autologous human serum (striped bars). Macrophage fluorescence was measured by flow cytometry and the average percentage of FL1-positive macrophages after removal of adherent platelets are shown for 3 independent experiments for each sample. Data for control samples are repeated in each panel, and compared to treatment with (A) latrunculin (1 ug/ml), (B) thrombin (0.1 U/ml), or (C) fucoidan (250 ug/ml). Statistically significant differences are indicated with their corresponding p-values. The addition of 10% serum did not result in any statistically significant difference (NS).
We also used flow cytometry to more accurately examine the level of platelet activation in different culture conditions. Platelets were analyzed for expression of P-selectin, an alpha granule component expressed during early platelet activation, and phosphatidylserine, a membrane lipid exposed on the surface of completely (and irreversibly) activated platelets. Incubation in serum-free media alone for 1 hour resulted in an approximately ten-fold increase in P-selectin expression but did not induce surface expression of phosphatidylserine (Table 1). Treatment of platelets with thrombin, known to cause complete degranulation and irreversible platelet activation [23,37], resulted in even higher levels of P-selectin and also increased surface expression of phosphatidylserine (Table 1). Interestingly, the data in Figures 1 and 2 showing phagocytosis of platelets incubated only in RPMI media suggests that only partial platelet activation, in the absence of complete degranulation or phosphatidylserine exposure, is sufficient to trigger phagocytosis. Although phagocytosis was enhanced when the platelets did express phosphatidylserine, we conclude that surface exposure of phosphatidylserine is not an absolute requirement for phagocytosis of platelets.
Table 1. Platelet Activation in Co-Culture Conditions
Inflammatory Cytokines are Enhanced Following Platelet Phagocytosis
The hypothesis that macrophage phagocytosis of activated platelets results in an inflammatory response that differs from the response following phagocytosis of apoptotic cells was tested by measuring the secretion of cytokines following addition of platelets or apoptotic cells to LPS-stimulated hMDMs. Autologous platelets in two different activation states were used in the co-culture experiments: platelets that were "partially activated" (with surface exposure of measurable quantities of P-selectin and CD40L) by preparing in serum-free media or "irreversibly activated" (with phosphatidylserine exposure in addition to P-selectin) by treatment with the calcium ionophore A23187 [37].
The inflammatory response of the hMDMs was assessed by measuring the levels of TNF-α, IL-6, and IL-23 after incubation with autologous primed platelets, autologous activated platelets, or control apoptotic leukocytes in the presence of LPS for 24 hours. As an additional control, we analyzed "platelets-only" cultures using the same media and incubation times as the platelet-macrophage co-cultures and were unable to detect any TNF-α, IL-6, or IL-23 in platelets alone (with or without LPS, data not shown). We therefore conclude that the cytokines secreted in this system are macrophage-derived, and in each experiment the cytokine levels were normalized to the amount of cytokine secreted by hMDMs incubated with LPS alone.
When compared to LPS stimulation alone, macrophage co-incubation with apoptotic cells inhibited LPS-induced secretion of all three pro-inflammatory cytokines (Figure 3A, grey bars). However, co-incubation with primed or activated platelets enhanced macrophage secretion of TNF-α, IL-6, and IL-23. Induction of pro-inflammatory cytokines in the presence of platelets was 20-60% higher than the levels obtained by LPS treatment alone. Furthermore, the macrophage cytokine secretion was enhanced to a similar degree after co-incubation with both partially activated and degranulated platelets (Figure 3A). These data suggest activated platelets enhance LPS-induced macrophage cytokine secretion even when they present phosphatidylserine to the macrophage.
Figure 3. Cytokine analysis of macrophages in the presence of activated platelets, apoptotic cells, and dexamethasone. Cytokines were measured by ELISA 24 hrs after stimulation with LPS (100 ng/ml) in the presence or absence of (A) apoptotic cells or platelets, and (B) dexamethasone alone (1 μM) or dexamethasone-loaded platelets. Data for activated platelets is repeated in both panels. Cytokine levels are expressed relative to treatment with LPS only. Macrophages were incubated with equivalent numbers of platelets in each condition. Shown are the averages of at least 3 independent experiments. Statistically significant differences are indicated with corresponding p-values.
Based on the knowledge that platelets can bind glucocorticoids via glucocorticoid receptors [38], we tested the hypothesis that glucocorticoid-bound platelets would be less inflammatory than platelets that are activated, but otherwise unmodified. Platelets were incubated with dexamethasone, then unbound glucocorticoid was removed by washing the platelets in citrated saline. Although it is unlikely that all of the dexamethasone becomes bound to the platelets, complete retention of the dexamethasone would yield a final concentration in the DEX-plt stock of 50 μM and a final (effective) concentration in the platelet-macrophage co-cultures of 1 μM. As shown in Figure 3B (striped bars), the levels of cytokines produced after co-culture with dexamethasone-loaded platelets were inhibited to 30-50% of the levels produced by stimulation with LPS alone. The dexamethasone-loaded platelets had a similar effect on cytokine secretion as 1 μM dexamethasone alone. These results indicate that the pro-inflammatory platelet effect on macrophage activation can be reversed by pre-loading the platelets with glucocorticoids.
Phagocytosis is an important means of clearing both immunologically compromised and apoptotic cells. Monocyte-derived macrophages are efficient phagocytes in organs of the reticuloendothelial system and within injured tissues; however, the process of platelet clearance by macrophages is poorly understood. This work has demonstrated phagocytosis of fresh autologous activated platelets (AAPs) by monocyte-derived macrophages using an entirely human-derived in vitro system. In this system, uptake of freshly isolated platelets is dependent on actin polymerization, but occurs independently of any soluble serum factors. Additionally, phagocytosis of platelets is enhanced with platelet activation.
Previous studies on platelet phagocytosis have focused on modified platelets such as chilled platelets, opsonized platelets, and aged platelets [39-46], each of which involves distinct changes to the platelet surface. Aged platelets most closely resemble freshly activated platelets because during aging platelets increase expression of phosphatidylserine and P-selectin [20]. Interestingly, Brown et al. have also shown in vitro that phagocytosis of aged platelets is mediated by scavenger receptors [20]. Therefore, macrophages may recognize freshly activated platelets in the same way that they clear aged platelets. The finding that phosphatidylserine exposure is not a requirement for phagocytosis of AAPs in the present study was somewhat unexpected, considering that phosphatidylserine is a well documented 'eat me' signal for the phagocytosis of many different types of cells undergoing apoptosis [47-50].
Because phagocytosis correlates with platelet activation, we would expect no phagocytosis to occur in the presence of quiescent platelets. However, RPMI media alone causes platelet activation (Table 1), probably due to the presence of low levels of calcium and phosphate. Additionally, the use of platelet inhibitors such as aspirin, EDTA, or prostaglandin, which may have maintained the platelets in a resting state, could not be used because they directly affect macrophage function [51,52]. Thus, one disadvantage of our system is that the platelet-macrophage interaction using activated platelets could not be compared to an interaction in which the platelets were in a truly resting state. Nonetheless, the interaction involving activated platelets is relevant because platelets are most likely activated at sites of tissue injury and perhaps during removal in the spleen. Thus, the interaction involving activated platelets was the focus of this work.
The macrophage response following phagocytosis of cells expressing surface phosphatidylserine is usually immunosuppressive [2,26,27,53,54]. In the present study, co-culture with apoptotic cells inhibited production of pro-inflammatory cytokines by LPS-activated macrophages. These results are in agreement with previous findings for TNF-α, IL-1β, IL-8, IL-12 [2,4,55,56], and are extended to now include IL-6 and IL-23.
In contrast to the effect of apoptotic cells, activated platelets enhanced pro-inflammatory cytokine secretion from LPS-activated macrophages. The cytokines measured in the current study, TNF-α, IL-6 and IL-23, are significant readouts because they are known to be secreted by macrophages, but not platelets, and they play important roles in mediating pro-inflammatory responses. Interestingly, the pro-inflammatory cytokine secretion was also enhanced by platelets with surface phosphatidylserine exposure. The finding that degranulated platelets, washed free from their secreted proteins, also enhanced LPS-induced macrophage cytokine secretion suggests that a secreted platelet factor is not likely to be responsible for this effect. However, a secreted platelet factor could exert the observed effect if it remained bound to the platelet surface after secretion from the platelet. Because the platelets remained in the co-incubation for the entire experiment (24 hrs), the possibility also exists that the inflammatory consequences of platelet-macrophage interactions occur independently of phagocytosis. Cell contact itself could be responsible for the observed effects. Nonetheless, we have shown the pro-inflammatory effect of platelets does occur in conditions which favor platelet uptake (Figure 1).
Recent studies have highlighted additional roles of platelets beyond hemostasis, particularly with respect to platelet-mediated effects on inflammation [28,57,58]. These results are particularly relevant to chronic inflammatory diseases, during which macrophages may interact with apoptotic or necrotic cells, as well as platelets, for prolonged periods of time. Studies in mice have demonstrated that depletion of platelets or platelet proteins affects macrophage infiltration and inflammation in lesions of the skin, joints, gut, and vasculature [59-63]. Although the precise mechanisms by which platelets impact macrophage activation remain unclear, the current study provides direct evidence, using human cells, of specific macrophage cytokines that are enhanced by activated platelets.
Pro-inflammatory cytokines secreted by macrophages can also exert effects on surrounding cells and tissues. For example, IL-6 and IL-23 stimulate T-cells for induction of Th17 immune responses, which are operant in autoimmune diseases such as inflammatory bowel disease, lupus, psoriasis and arthritis [64-67]. We speculate, therefore, that in addition to amplifying general pro-inflammatory responses, platelet-macrophage interactions might also play a role in Th17-mediated autoimmune diseases.
Glucocorticoids such as dexamethasone can exert powerful immunosuppressive effects on leukocytes and are thus an attractive therapy for modulating inflammation [68]. After steroid binding to glucocorticoid receptors, which occurs within the cytoplasm, activated glucocorticoid receptors translocate to the nucleus and inhibit transcription of a variety of pro-inflammatory cytokines [68]. We speculate, therefore, that the immunosuppressive action of dexamethasone-loaded platelets occurs by facilitating delivery of dexamethasone to macrophage glucocorticoid receptors. Because macrophage glucocorticoid receptors are cytoplasmic, we further speculate that the immunosuppressive effect of dex-platelets is a result of phagocytosis. The use of dexamethasone-loaded platelets for modulating macrophage action may prove useful in treating diseases characterized by excessive and unresolving inflammation. Our results demonstrating similar levels of immunosuppression with both free dexamethasone and dexamethasone bound to platelets suggests that tethering glucocorticoids to platelets may increase drug targeting and reduce the need for high systemic doses of glucocorticoids, which can have unwanted side effects [69]. Furthermore, given the role of IL-6 and IL-23 in Th17-mediated inflammatory responses, the platelet-macrophage interaction is therefore a rational pharmacological target for inhibiting some Th17-related diseases.
We have shown here that the interaction of human macrophages with autologous platelets results in scavenger-receptor-mediated platelet uptake and enhancement of LPS-induced cytokine secretion. Given the presence of activated platelets together with macrophages during the response to injury and during inflammation, activated platelets at sites of inflammation most likely exacerbate the macrophage response. The presence of platelets must therefore be carefully considered when studying the cellular interactions occurring in inflammatory lesions.
We have also presented evidence here that platelets can be engineered to exert anti-inflammatory effects on macrophages. Given the emerging role of platelets in inflammatory diseases, the possibility of reversing macrophage activation with dexamethasone-loaded platelets is a promising therapeutic approach to treating unresolved inflammation.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
CMS conceived of the study, participated in its design and coordination, performed phagocytosis assays and cytokine measurements, and drafted the manuscript. WDH participated in the design of the study, coordinated the selection and participation of blood donors, and processed the blood samples. THF was the principal investigator on this project and provided guidance and advice on the experiments and manuscript. All authors read and approved the final manuscript.
This work was supported by sponsored research funds from Entegrion, Inc. (Research Triangle Park, NC, USA) and a collaborative research grant from the North Carolina Biotechnology Center.
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Obama wants background checks on you
Posted By Joseph Farah On 01/20/2013 @ 5:18 pm In Commentary,Opinion | No Comments
What's the definition of irony?
How about a sitting president who, after four years, has provided next to zero validated information about himself demanding that citizens of the United States undergo more federal background checks to exercise their most basic Second Amendment rights?
That's just one of the outrages I find in the 23 executive orders issued by the White House designed to discourage law-abiding U.S. citizens from purchasing firearms for their own self-defense and to ensure that tyrants like himself have a monopoly on force in our country.
Sure he wants background checks on all firearms purchases. Why? Because the government wants to know where all the guns are. Never mind that the tragedy that prompted this government and media hysteria for more gun control would not have been averted with all the background checks in the world. The shooter stole his weapon.
But turnabout is fair play.
Background checks?
I think the American people deserve to see some background checks on Barack Obama.
Here's what most Americans would like to see:
Now, you can agree with me and Sheriff Joe Arpaio on the last one or not. But it really doesn't matter. In fact, I'm even willing to close the chapter on the birth certificate matter. Though the question remains, how is this guy eligible to be president when he, according to his own story, is the son of a Kenyan goat herder and an American woman too young to bestow natural born citizenship on her son – no matter where he was born? And why all the secrecy about something as innocent as a birth certificate?
But forget that, for a moment. Nobody cares. It's water under the bridge. Obama has, among other things, successfully redefined the constitutional requirement to serve as president.
Still, Obama has more secrets than any would-be gun purchaser I have ever met in my life. Yet he has Secret Service protection. He obviously sees the value of armed defense or he would dispatch those agents tout suite. He obviously cares about the safety of his own children because they are provided with armed security, too. He just doesn't think your kids should have any – even if his is provided courtesy of the taxpayers of the U.S. and yours is provided with your own money and time.
So, I say it's time for Obama to submit to some public background checks.
How is it that with so many secrets regarding his life and identity, he has the cojones to demand background checks on Joe Q. Citizen, who simply wants to exercise his constitutional rights to protect his life, liberty and property?
He wants background checks on me?
My life is an open book.
He wants background checks on you?
Are you asking to handle highly classified documents like him? Or are you just being a responsible citizen and watching out for your family – at no expense to the taxpayer?
I'm shocked that no one else has seen the irony in the only man one could honestly characterize as the "Manchurian President" demanding background checks on ordinary, hard-working, law-abiding Americans to whom he is pledged serve.
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text: The Christmas season is here and as an adult I am supposed to be completely stressed out. Shopping, family gatherings, overeating and Yankee swaps are supposed to push me to the edge. I am supposed to feel bad that I am not a kid anymore and get depressed that my quest to recapture the innocence of my childhood will never be fulfilled. I say "Bah Humbug" to all that. The Christmas season actually makes me feel really good and, frankly, I think kids are the one who experience the most stress during the holidays.


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Tenerife Tourism - Beaches - Attractions - Tours - Activities - Golf
Either click on the links below or scroll down
- Statistics
- Protected Areas
- Disabled Tourists
- Flights
- Accommodation
- Beaches
- Attractions and Excursions
- Tours
- Activities
- Golf
- Eating Out
- Nightlife
- Shopping
- Health care
Tourism is of major importance to the economy of Tenerife.
Statistics show that the tourism attracted almost five and a half million visitors during 2006 and together with related service industries tourism generates more than 60% of GDP.
Of those visitors, approximately 33% were from the United Kingdom, 27% were from the Iberian Peninsula and 12% were from Germany. The remaining 28% were spread between Ireland, Holland, Belgium, France, Italy, Sweden, Finland, Denmark, Norway and Russia.
According to the 2006 statistics Tenerife has just over 185,000 beds, 46% of which were in hotels and 54% were in self catering accommodation.
Protected Areas
The fragile Eco-system of Tenerife has been of great importance to the Government and almost 45% of the island is protected to preserve the National Heritage.
The enormous Mount Teide National Park was created in 1954 but there are also the Coronal Forestal Nature Park and the Anaga and Teno Rural Parks. During 1994 the Canary Islands Natural Areas Act defined different categories of protection and planning regulations to protect the island's natural resources and introduced penalties to guarantee various objectives.
The Mount Teide National Park, at an altitude of over 200 metres was designated as a UNESCO World Heritage Site during 2007. The committee took into account that the park scenery is uniquely spectacular site attracting four million visitors per year.
The natural Tenerife habitats for extensive flora and fauna remain well protected but a network of established paths and thirty recreation zones within the protected areas still make enjoyment of the environment possible.
There are some camp sites with good facilities within the protected areas but it is necessary to obtain a permit from the Department of the Environment, Tenerife Cabildo.
Disabled Tourists
Tenerife seems to have catered very well for disabled travellers. There a number of companies specialising in disabled airport transfers and excursions; wheelchair and electric buggy hire.
Most of Tenerife holiday accommodation has been designed for wheelchair access and the resort of Los Cristianos is almost totally accessible by wheelchair. The number of disabled tourists visiting Los Cristianos is remarkable.
There are two airports on Tenerife, Los Rodeos Airport is located in La Laguna in the north and Reina Sofia Airport is located in the south. Both are busy commercial airports with significant traffic.
Los Rodeos Airport is approximately an hour by car from the south of the Island and Reina Sofia is about 20 minutes by car from Los Cristianos so it is important to bear this in mind when booking flights.
Los Rodeos Airport mostly handles domestic inter-island flights with some international connections, there are 13 airlines operating form this airport including;
Air Berlin - Air Europa – Clickair – Iberia – Lufthansa - Spanair and Monarch.
Reina Sofia Airport mostly handles international flights to the major cities of the world, over 50 airlines use this airport which handles almost 9 million passengers a year.
Airlines using Reina Sofia Airport include;
Aer Lingus - Air Europa – Easyjet - First Choice – Globespan - Iberia - Jet2.com Monarch - My Travel - Ryanair – Spanair – Sterling - Thomas Cook – Thomsonfly – Topfly - Windjet and possibly new owners of XL Airways
There are already many foreign property owners in Tenerife, many will allow their family and friends use their accommodation.
The choice of hotel accommodation in the Tenerife tourist resorts is very good ranging from basic to five star. Self catering units are also available in all the resorts ranging from basic studios to luxury villas with private swimming pools.
Tenerife attracts many independent travelers who book their accommodation through various means; the internet is now probably the most popular way booking flights and accommodation. Others sources are private adverts, the local papers usually have many classified adverts for holiday accommodation.
Many people use travel agents to make their arrangements including flights and accommodation and although sometimes more costly, the availability of "all inclusive" deals can actually save money.
The peak Tenerife holiday season is from Christmas to Easter with many people enjoying a winter sunshine break; the choice of accommodation may be limited during this period so it is advisable to book early.
The beaches of Los Cristianos, Las Americas and Las Vistas in between are all sandy and have great facilities, sun beds, parasols etc. Some have water sports activities and of course they are in the actual resort areas so bars, restaurants and shops are always close at hand. The waters are calm and swimming is fairly safe, lifeguards are usually on duty.
Other notable sandy Tenerife beaches are Playa Tejita, (sometimes known as Red mountain beach), a natural sandy beach just past Los Abrigos, or Playa El Medano just the other side of the Red Mountain. Both of these beaches are outstandingly beautiful but strong winds sometimes make sea conditions dangerous for swimmers. These beaches are very popular wind and kite surfers.
To the north of the island, past Santa Cruz there is a man made sandy beach known as Playa de Teresitas which is very popular with the inhabitants of Santa Cruz. The beach has many tropical palm trees and sits beneath dramatic steep mountains and cliffs.
There are numerous other beaches all around the coast of Tenerife, some are in the smaller coastal towns and villages with good access, others are not so accessible and many are stony.
Attractions and Excursions
Tenerife is a successful holiday destination, apart from enjoying the weather there are many interesting places to visit and activities to suit all people of all ages. Even if you live on the island you could never be bored, the choice is amazing and there are always new things to discover.
The following Tenerife attractions and excursions can be booked through the many local agents with transport included. If you are staying in a hotel or apartment complex the reception will often make the arrangements.
A sample of some of the most popular Tenerife tours available on the Island;
- Mount Teide and Las Canadas Spectacular coach tour, optional cable car to summit.
- Masca Valley, visit the village that time forgot, spectacular views.
- La Gomera Ferry crossing and coach tour of the Island and national park.
- Tenerife Island Tour Coach tour with stops in Candelaria, Santa Cruz, Puerto de la Cruz, Icod de Vinos and Los Gigantes.
There are numerous Tenerife activities for all ages, here is a sample of the most popular;
- Aqua Park Water park with slides and rides, also dolphinarium.
- Big Game Fishing Tuna, Barracuda, Marlin and other large species.
- Diving Various schools and facilities.
- Dolphins Various trips form most harbours, glass bottom boat trips.
- Freebird Sailing Various trips, see the Dolphins and Whales.
- Go Karting More than one circuit to choose from.
- Jungle Park Zoo and Botanical Park with Birds of Prey show.
- Loro Parque Trip to Puerto de la Cruz to see the Zoological gardens, tropical birds, lions and other animals aquariums, dolphins and penguins.
- Medieval Night In the castle at San Miguel with banquet and ballroom with live show.
- Pirate Ship The voyage of a lifetime.
- Quad Bikes Off road exploring
- Siam Park The new oriental themed water park.
- Submarine Safaris Explore the underwater world.
- Trekking Various options including Masca.
- Water Ski-ing Various locations, paragliding too.
- Mountain Biking
Tenerife has a good choice of golf courses to suit all levels of play.
Tenerife South
- Abama Exceptional 18 hole golf course created by Dave Thomas.
- Amarilla Beautiful 18 hole golf course created by Donald Steel.
- Costa Adeje Unusual 18+9 hole golf course designed by Pepe Gancedo
- Golf del Sur Interesting 18+9 hole golf course designed by Pepe Gancedo
- Los Palos 9 hole golf course, perfect for beginners.
Tenerife North
- Buenavista Golf Cliff side 18 holes course designed by Seve Ballasteros
Eating Out
The choice of places to eat out sometimes makes the decision difficult. All the main Tenerife tourist areas have restaurants serving all types of international cuisine. Prices are competitive and standards are high.
Most restaurants display their priced menus outside the choice of dishes is exception in most restaurants. Fish dishes are very popular because of the abundance of fish. Fish and meat dishes are usually served with vegetables and Canarian potatoes, wine is optional of course and very inexpensive, especially if you drink the local wines which are very good.
The nightlife in Tenerife is laid back with many options to choose from in most areas.
Many bars have live music and cabaret where you can relax and have a drink whilst you are being entertained. If you look around the main areas you will probably be able to choose the type of entertainment you like.
Many of the venues employ cover acts ranging from Elvis Presley to Ronan Keating. There are quite a few Piano Bars and Jazz bars, particularly in Las Americas.
Most of the large hotels put on their own cabaret shows and visitors from outside the hotel are usually welcomed. Life in the Tenerife entertainment world is quite competitive so entertainment standards tend to be high.
There is no shortage of Nightclubs for those that want to stay out late, especially in Las Americas. The choice of clubs is varied so is the music ranging from Hip Hop and House to Swing. The night clubs are concentrated in one area and security is not bad. All age groups are mostly welcomed but the clubs are predominantly frequented by the younger generation. Entrance is usually free.
The range of shops would stimulate any shopaholic, prices are fiercely competitive and virtually duty free. There is a very upmarket shopping centre known as the Safari Centre opposite the Parque Santiago complex in Las Americas. This area has more designer shops than you could ever imagine, a shopping paradise.
There are numerous upmarket retail outlets selling designer clothes, jewelry, watches, cameras and electronic goods, all at very good prices. You will also find local Canarian wares including pottery, embroidery and hand-crafted goods.
Alcohol and Cigarettes are probably the best bargains in Tenerife because they do not attract penalty taxes in Tenerife as they do in some countries. Perfume is also a popular buy, virtually all the designer names are available at excellent prices.
Health Care
If you are a non-resident or tourist in Tenerife your are entitled to reciprocal health care under the terms of an EU Agreement between the members states. You will however need to produce a document from your own country prior to you visit which is usually obtainable through the Post Office. If you are a UK resident you will be given a "European Health Insurance Card" which contains a reference number.
If you need health care in you will need to visit one of the government health clinics or hospitals to obtain free health care but prescription medicines are not free.
There are numerous private health clinics all over the Island, especially in tourist areas. Although you will be charged, if you have travel insurance, you may be able to recover your costs subject to the policy terms and conditions.
See our selected best Tenerife tourism and holiday activity videos.


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>Sonora / Tuolumne News, Sports, & Weather, Angels Camp, Twain Harte, Jamestown | Union Democrat
Two girls' soccer teams brimming with talented high school players from around the Mother Lode League will clash Tuesday for the inaugural "Foothill Finale, North versus South All-Star Game" at Bret Harte High.
Mother Lode League Goalkeeper of the Year Larissa Barrett, of Summerville, makes a diving stop Tuesday at practice for an All-League game being played June 11 at Bret Harte High. Maggie Beck / Union Democrat
A "North" team comprised of the MLL's All-League MVP, First Team, Second Team and Honorable Mention selections from the Amador Buffaloes, Argonaut Mustangs and Linden Lions is set to face a "South" team made up of the corresponding picks from the Calaveras Redskins, Bret Harte Bullfrogs and Summerville Bears in the first-of-its-kind game that coaches are looking to make an annual tradition.
"Every athlete wants to play that one more game and they deserve it," said Bret Harte head coach Rosie Sundell during an interview while "South" team players practiced Tuesday at Calaveras High's Frank Meyer Field. "All-star games exist for other sports, so why not girls' soccer? I know they're going to have a good time and there's going to be some great soccer on display with this level of talent on the field."
Sundell and the league's coaches came up with the idea for the game after the season ended at a meeting to discuss this year's All-League selections. She saw all the names written on the white board and suggested they organize the MLL's first-ever all-star game.
And the other coaches at the meeting liked Sundell's idea, including Calaveras head coach Rob Leetham and Summerville boss Brad Schultz.
"For years, I've always thought how fun an all-star game would be," Schultz said, "and when Rosie mentioned it, I thought, 'Yes, let's get this done!' "
Sundell said that by the end of the meeting, the six MLL coaches had formed a basic plan for how they would go about getting the new event off the ground and then started putting those plans into action.
However, the event is still a work in progress. Sundell said there may be a small admission fee that would be used to fund future all-star games, but those details have yet to be finalized with the school.
"It's a lot of work organizing something like this for the first time," Schultz said. "We have to make sure we aren't violating any rules and that everything is covered, but it all seems to be going OK."
Players for the "South" who practiced Tuesday at Calaveras High were excited for the unique opportunity to play on the same team as their rivals.
"I never thought I would ever get to play with some of these girls," said Bullfrog defender Diana Munoz. "It's definitely going to be a fun — and different — experience."
The "South" team features the MLL's Forward of the Year Tiffany Souza, of the Redskins, and Goalkeeper of the Year Larissa Barrett, of the Bears, while the "North" squad includes Defender of the Year Alex Giannecchini, of the Lions, and Midfielder of the Year Madissen Ostergaard, of the Buffaloes. Ostergaard helped lead Amador to the Sac-Joaquin Division VI title and a share of the league championship with Calaveras.
Despite her season having ended weeks ago in the first round of the Division V Championships, Souza said she was "fired up" to face the girls from the "North" squad, especially Ostergaard, for one last time in her high school career.
"I think we have a really good shot at doing well," Souza said. "I already have confidence in the girls from my team, and I know there are some ruthless girls from Bret Harte and Summerville who are going to be assets."
The Union Democrat is now online in a Replica E-edition form and publishes Tuesday through Saturday. E-Edition or Print Plus subscribers have full access.
If you are not a current subscriber, subscribe today for immediate access. Circulation: 209-533-3614
Weekly Arts and entertainment guide for Calaveras and Tuolumne counties
Available online every Friday or get it early in Thursday's print edition.


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By IBD Staff01/10/2013 04:35 PM ET
Stocks climbed Thursday, with the S&P 500 hitting a five-year high as good news from abroad painted a picture of a healing global economy.
The S&P 500 added 0.8%. The index hasn't seen these levels since last September — and it hasn't closed this high since December 2007.
The Dow Jones industrial average rose 0.6% and the Nasdaq gained 0.5%. Volume was mixed: Nasdaq's action fell a smidge but the NYSE's increased, according to preliminary data.
The good news was indeed good: China's exports rose to their highest in seven months in December. That's nice for China. But more importantly, the data suggest an improvement in global demand, an area of great concern in recent months. Looking at China's exports as a barometer of the world economy, and you'll be heartened by this report.
Separately, the European Central Bank kept its benchmark short-term interest rate steady at 0.75%. Some analysts had expected a rate cut. ECB President Mario Draghi said he expects the eurozone's economy to improve later this year. Upbeat comments from the beleaguered ECB chief have been few and far between, and Thursday's remarks caught some off-guard.
LinkedIn (LKND) shot up 4% in 46% above-average volume. The stock cleared a 116.97 buy point in a cup-with-handle base. CLSA started coverage of the professional social networking website operator with an outperform rating and a $133 price target.
Celgene (CELG) added 3% to a fresh record high. The stock has been on a hot streak since staging an upside reversal at its 50-day line Dec. 31. It's now 17% past an 81.34 buy point. Earlier this week, the biotech announced positive trials of a psoriasis drug and outlined rosy long-term financial targets. Celgene aims to double sales by 2017.
Urban Outfitters (URBN) surged 5% in active trading. Earlier Thursday, the retailer reported total holiday season sales that jumped 15% over the same period last year. Comparable retail segment sales rose 9% in November and December, boosted by gains in the company's direct-to-consumer channel. Minus that, same-store sales slipped 1%. The stock cleared a 40.75 cup-with-handle buy point Jan. 2. It is now about 4% above that mark.
Femsa (FMX) added 2% to a record high. The lift puts it 7% past a 98.20 buy point in a flat base. The Mexican firm is the largest Coca-Coca bottler in the world. In November, the company bought a 75% stake in Farmacias YZA, a leading drugstore chain. The deal is expected to close in the first quarter of this year.
Comments reported by Reuters from Deutsche Telekom (DTEGY) CEO John Legere about a pending iPhone from T-Mobile USA helped send Apple (AAPL) ahead 1%. The comments may also have helped boost IBD 50 stock ARM (ARMH), which surged 5%. That's a new high and 43% past a 29.29 buy point from a breakout in October.
U.S. Silica (SLCA) swooned 6%, erasing all of Wednesday's gain and then some after being cut to hold from buy at Jefferies. The stock is now 4% past a 17.35 buy point cleared Jan. 2. U.S. Silica broke out in weak turnover.
Michael Kors (KORS) slipped 2%. The Hong Kong-based fashion designer has been struggling to hold above its 10-week line as it shapes a possible base. Much of that base has formed below the line, a sign of potential weakness.
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How do I download the data?
- Visit data.medicare.gov - Opens in a new window to get the downloadable data.
- Get archived Home Health Compare data - Opens in a new window.
About using government data
Works of the U.S. Government are in the public domain and permission isn't required to reuse them. An attribution to the agency as the source is appreciated. Your materials, however, shouldn't give the false impression of government endorsement of your commercial products or services. See 42 U.S.C. 1320b-10.


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American Embassy
If you lose your passport, a visit to the American Embassy is necessary to get a new one. First, file a police report and then bring the report as well as a photocopy of your passport and a few passport-sized photos of yourself to the embassy.
The American Embassy is located at 2 rue St. Florentin in the 1st arrondissement (metro: Concorde) and is open from 9 a.m. – 1 p.m. from Monday to Friday, except for French and American holidays.
The American Embassy web site has information on services and procedures.
Foreign Exchange
There are foreign exchange offices on just about every major thoroughfare in Paris. They are open during business hours; though don't be surprised if some are closed for lunch or for a holiday. If you need money for that last glass of côte du Rhône one night, the ATMs of local banks can provide you with money from your bank account at any hour. The local bank often charges a small fee in addition to the potentially hefty fee charged by your bank at home. Contact your bank to find out the exact surcharge.
The American Hospital
If you find yourself in need of medical care and anxious to talk to an English-speaking doctor, visit the American Hospital of Paris. It is located at 63, boulevard Victor Hugo in Neuilly, just outside of Paris proper. Take the metro line 1 to Porte Maillot, then get on bus #82. The Hôpital Américain is the last stop.
If you only have a cold, walk into any pharmacy (distinguishable by the green neon crosses outside of the shop) and describe your symptoms to the pharmacist as best you can. French pharmacists can normally suggest the right treatment for minor ailments.
Tourist Information
Since Paris is the world's premier tourist destination, you will not have to look very hard for tourist information. The main office of tourism is located at 127, avenue des Champs élysées, near the Charles-de-Gaulle-étoile metro stop.
Places to Eat, Drink and Stay
These days of the strong euro mean that eating in fine restaurants is not as easy as it used to be. Good deals can still be found on prix fixe menus (sometimes also called a formule), where you pay a single price for 2 or 3 courses and wine. One can do especially well at lunchtime.
If you speak French, consider picking up a copy of Paris pas cher (roughly translated: Paris on the cheap). It's an annual guide to the best values on everything from a meal out to a haircut.
Eating Out
While it's been too long since my last trip to Paris for me to recommend a restaurant I am sure will be good, friends recommend Chez Ramulaud for an excellent traditional French meal at a great price (prix fixe menu at 35-50€).
Chez Ramulaud
269, rue du faubourg Saint-Antoine
Metro: Faidherbe
Night Out
In the style of the East Village of Manhattan, hip young Parisians have invaded the blue-collar 11th arrondissement. Stop by Café Charbon early into your evening (before the crowds make it uncomfortable) and then head to Cithea, where you'll find live musical performances and DJs at very reasonable prices.
Café Charbon
109, rue Oberkampf
metro: Parmentier
114, rue Oberkampf
Where to Stay
If you arrive in the summer, you may find that all of the places listed in your guidebook are full. This is especially true if you use one of the Lonely Planet guides. In my experience, the best course of action is to travel to a neighborhood that interests you and find a hostel/hotel that matches your budget. You may hit upon an amazing, wonderful place or, more likely, you will find a place that is acceptable. Rooms without en-suite bathrooms and showers are always cheapest and a good way to save money. France has a star system (1-4) to rate hostels/hotels. One star might be termed "acceptable," 2 "economical," 3 "comfortable" and 4 "luxurious." If I am not already familiar with a hostel/hotel, I try to make sure it has at least a 2 star rating.
If you don't mind being a little far from the center of the city, one of my favorite places to stay is the Hotel Dorée in the 18th arrondissement. It is located near Montmartre and Sacré Coeur in a predominantly African and Arab neighborhood. The owners are a friendly older couple. The husband enjoys painting and has decorated the rooms with his work. Because the hotel is somewhat outside the city center, the room prices are quite reasonable (double bed with shower is 64€).
Hotel Dorée
66 Boulevard Barbès. Metro Marcadet-Poissoniers or Barbès Rochechouart. The hotel has a web site, but it does not work well.


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Get the latest Flash player
Date: 5 years ago
Runtime: 3:24
Views: 41,498
Top Comments
All Comments
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The buzz continues to build for a community favorite - the HEAL Empty Bowl Event. Competitors for the Nest Best Soup contest are amping up their signature soups to present to the public at Mt. Annie's from 4-7pm this Sunday, October 28th. Each entrant will be present to serve their soups and answer questions about its origin, ingredients and maybe even share a tip or two about making great soup.
The entrants for the Nest Best Soup are Zocca Coffee, Laura Weaver & Elizabeth Frey on behalf of JBarJ Church, IGA Lawrence Brothers, Schlotzkys, Senior Citizen's Center in Ruidoso Downs, Bonito Park, Pena's Place, Paul Kolb and West Kelly on behalf of the Disabled Skiers Program, LPN Jessica McWhirter from Artesia, Mario's Market, Christie Herrera, staff and residents of the NEST, Village Buttery, Sue Lovett Francis, Britta Magnusson, Mrs. Ruidoso 2012 Amber Buster, Sue Hutchison, "Veggie Girl" Angie Fernandez, The Olive Oil Shoppe, Billy The Kid Casino, JR from Dreamcatcher Catering and C and C Consulting and Alto Lake Golf & Country Club.
The Nest Best Soup competition is the brainchild of HEAL volunteer Chef Brendan Gochenour. Gochenour has been approaching various Chefs, restaurants, foodies and organizations about entering the contest. The public will select the Best Soup by voting on the ones they sample. The winner of the competition will receive bragging rights for a year and be presented with a Nest Best Soup plaque to display in their home, office or restaurant. In addition to helping her husband solicit soups, HEAL volunteer Audrey Gochenour is managing the production of cookies and asks local residents to help by donating a dozen or two.
Tickets to the HEAL Empty Bowl Event are $15 and include a handmade pottery bowl, soup tastings, crackers, ice tea and dessert, which will be homemade cookies made by the HEAL committee, various community members, groups, Nest staff and residents.
There will also be a limited number of artisan bowls available, made by local artists specifically for this event, for an additional $10. A large silent auction will be occurring during the Empty Bowl, featuring beautiful and unique pieces of art and local business gift certificates.
Business spotlight
Many thanks to Alan Miner and Renne Bradley from the Gazebo Potters in midtown for coordinating with the community to assist in making bowls for the HEAL Empty Bowl Event. Both artists were very accommodating to artists of all skill levels, going as far as to teach people with no experience how to make a great bowl for this charity event. Miner and Bradley also went inside the Nest Domestic Violence Shelter to work with the Nest residents so they too could make bowls. The children especially loved this activity and were still talking about "their" bowls the next morning. Thanks Alan and Renne!


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Sean's Ships: Sail to Steam
Ep 6: Love ships and all things nautical? Sean certainly does and along with the help of his trusty Boatadex he is going to design the best ship ever!
This time Sean and Boatadex take a guide from sail to steam boats!
Player loadingDownload File


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Narrow your search
Reddit just joined the original-video scene. With its first series of documentary shorts debuting Wednesday, Reddit wants to raise the profile of its brightest communities and appeal to more outsiders.
Will my Verizon iPhone 5S work on AT&T? That's a question asked by one Ask Maggie reader confused by the puzzling state of device interoperability. This week's column tackles the issue.
The complex system of white matter fibres that relay information in the brain has been accurately recreated in a 3D-printed model.
The plant to make strong iPhone screens will open this month with 700 full-time jobs and 1,200 temporary construction jobs, Bloomberg reports.
The US National Security Agency placed malicious software on more than 50,000 computer networks around the world, says a report based on documents leaked by Edward Snowden.
CNET tracks all the Apple iPad 3 rumors -- from the likely to the crazy -- that we've heard so far.
What does Google's new "instant" search result page mean for users and for advertisers? We explore the new system with search experts Tom Krazit from CNET, and Danny Sullivan from Search Engine Land.
Wiseguys' Theo Keating melds deceptive dance-style tracks with Simian Mobile Disco's Simon Lord's sinister lyrics that alchemically transmute the otherwise alluring dance-pop elements. Download a free MP3 of "Any Way You Choose To Give It" courtesy of CNE


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- Early Learning
- Active Play
- Tunnels
- Tunnels of Fun Super Play Jumbo Junction Set
Tunnels of Fun Super Play Jumbo Junction Set
Large View | View Related Items
Product Description
Create creative play spaces! Tents, junction, and tunnels attach to each other in various combinations for instant hide-and-seek fun. Pieces set up easily and fold compactly in only seconds for convenient storage. Easy-to-clean, moisture-proof polyethylene floor. Mesh center for visibility and ventilation. Nylon taffeta. Includes a middle "junction" connecting point, four 3-ft. x 18" dia. tunnels, and nylon storage bag. Tunnel junction measures 18-1/2" H x 35-1/2" dia. Ages 3-6.


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white sapphire and silver pear earrings
designer's notes
Classic with modern touches, Judith Ripka's designs will remain a true favorite among your jewelry collection.
color: Silver Ridged sterling silver Twisted front post with tear drop white sapphire stone Teardrop drops with white sapphire pave stone center Approximate total white sapphires 0.108 ct Post with omega clasp Measures ⅜'' wide x ⅞'' long All measurements are approximate 14 Day Return Policy; returns must be received within 14 days. No returns will be accepted on international orders. See FAQ for more details. Sterling Silver / White Sapphire; U.S.A; style# 319896601


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GDK Enterprises, Inc. (dba GDK Technologies, Inc.)
|GDK Enterprises, Inc. (dba GDK Technologies, Inc.) Patent applications
|Patent application number
|TREMOLO ASSEMBLY - A bridge assembly having a saddle plate with a first aperture, and a tremolo block with a second aperture aligned with the first aperture. A bushing extends through the first and second apertures and is rotatable relative to the tremolo block. The bushing has an internal bore with a longitudinal axis and being substantially concentric with the tremolo block's second aperture. An engagement member extends through the bushing and is partially disposed in the internal bore. A tremolo arm has an engagement section with a partially cylindrical shape and a flat engagement surface that defines a plane substantially parallel to a longitudinal axis of the engagement section. The engagement member is perpendicular to and laterally disposed from the internal bore's longitudinal axis. The engagement member is in frictional engagement with the flat engagement surface when the engagement section is disposed in the internal bore. The tremolo arm's engagement section rotates with the busing as a unit relative to the tremolo block.


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IM Global, EMJAG team up for Stallone thriller Headshot
IM Global will commence pre-sales in Berlin this week on the Sylvester Stallone action project Headshot.
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Parents are raising concerns about the lack of seat belts on school buses; this after a crash in Georgia critically injured two students.
According to local Laidlaw officials, school buses use a passive restraint system known as Compartmentalization, which they compare to an "egg in a carton." So if an accident occurs the child is bounced around in a cushioned seat and not held rigid by a seat belt.
According to a study done by the National Highway Transportation Safety Administration, approximately 450,000 public school buses transport more than 23 Million children every year, and on average, six children die in school bus crashes each year. Four kids died in a Minnesota bus crash in February, the bus was not equipped with seatbelts.
Marvin Shipley of Laidlaw says, "N.H.T.S.A. did quite a study and the conclusion was that the compartmentalization was still better than the seatbelt."
Another study done by the National Academy of Science highlights that kids are at a greater risk in the bus loading zone then while they are on the bus.
KKCO firmly believes in freedom of speech for all and we are happy to provide this forum for the community to share opinions and facts. We ask that commenters keep it clean, keep it truthful, stay on topic and be responsible. Comments left here do not necessarily represent the viewpoint of KKCO 11News.powered by Disqus