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Inactivaion of tumor suppressor genes (TSGs) by promoter CpG methylation frequently occurs in tumorigenesis, even in the early stages, contributing to the initiation and progression of human cancers. Deleted in lung and esophageal cancer 1 (DLEC1), located at the 3p22-21.3 TSG cluster, has been identified frequently silenced by promoter CpG methylation in multiple carcinomas, however, no study has been performed for lymphomas yet.
We examined the expression of DLEC1 by semi-quantitative reverse transcription (RT)-PCR, and evaluated the promoter methylation of DLEC1 by methylation-specific PCR (MSP) and bisulfite genomic sequencing (BGS) in common lymphoma cell lines and tumors.
Here we report that DLEC1 is readily expressed in normal lymphoid tissues including lymph nodes and PBMCs, but reduced or silenced in 70% (16/23) of non-Hodgkin and Hodgkin lymphoma cell lines, including 2/6 diffuse large B-cell (DLBCL), 1/2 peripheral T cell lymphomas, 5/5 Burkitt, 6/7 Hodgkin and 2/3 nasal killer (NK)/T-cell lymphoma cell lines. Promoter CpG methylation was frequently detected in 80% (20/25) of lymphoma cell lines and correlated with DLEC1 downregulation/silencing. Pharmacologic demethylation reversed DLEC1 expression in lymphoma cell lines along with concomitant promoter demethylation. DLEC1 methylation was also frequently detected in 32 out of 58 (55%) different types of lymphoma tissues, but not in normal lymph nodes. Furthermore, DLEC1 was specifically methylated in the sera of 3/13 (23%) Hodgkin lymphoma patients.
Thus, methylation-mediated silencing of DLEC1 plays an important role in multiple lymphomagenesis, and may serve as a non-invasive tumor marker for lymphoma diagnosis.
Keywords:DLEC1; CpG; Methylation; Tumor suppressor; Lymphoma
Epigenetic silencing of tumor suppressor genes (TSGs) by promoter CpG methylation and histone modification has been widely recognized as one of the major causes of tumorigenesis including hematological malignancies [1,2]. Aberrant methylation of TSG is frequently detected even in the early stage of tumorigenesis, suggesting its potential as tumor biomarker for early detection and therapeutic targeting.
Deletions of the 3p22-21.3 region have been identified as one of the earliest molecular events in various malignancies [3], including naspharyngeal [4], head and neck [5], lung [6], gastric [7], breast [8], cervix [9] and renal [10] carcinomas, as well as lymphomas [11]. A growing number of TSGs, including RASSF1A[12,13], BLU/ZMYND10[14,15], and CACNA2D2[16], have been identified in this region [17,18]. Frequent inactivation of several 3p21.3 genes as functional TSGs, such as RASSF1 and BLU[14,19], by promoter CpG methylation had been identified associated with tumor initiation and progression. For example, promoter methylation of RASSF1A has been shown related to poor prognosis and advanced tumor stage of certain tumor types [20-33]. Restoration of RASSF1A in cancer cell lines inhibited tumor cell growth and metastasis [34]. Thus, 3p22-21.3 is a critical TSG cluster in tumorigenesis [3,18].
Deleted in lung and esophageal cancer 1 (DLEC1), a 3p22 cluster genes, was first identified as a TSG in esophageal and lung cancers [35]. Downregulation of DLEC1 by promoter methylation has been found in multiple cancers, including nasopharyngeal [36,37], ovarian [38], lung [39], hepatocellular [40], gastric [41], renal [42], and breast carcinomas [43], suggesting its potential as a broad TSG [44,45]. Remarkably, DLEC1 was methylated in breast cancer as well as pre-invasive lesions but rarely in normal breast tissues, indicating its potential as an epigenetic marker for early tumors [43].
In this study, we examined the expression and methylation status of DLEC1 in lymphoma cell lines and tissues, and evaluated its potential as a tumor marker for the early detection of hematologic tumors.
Cell lines and tumor samples
Non-Hodgkin and Hodgkin lymphoma cell lines studied included diffuse large B-cell lymphoma (DLBCL) cell lines (OCI-Ly1, Ly3, Ly7, Ly8, Ly18, SUDHL6); peripheral T cell lymphoma (PTCL) cell lines (Ly13.2, Ly17); Burkitt lymphoma (BL) cell lines (AG876, BJAB, Namalwa, Rael, Raji); Hodgkin lymphoma (HL) cell lines (L428, L540, L591, L1236, KM-H2, HD-LM-2, HD-MY-Z) (DSMZ cell collection, Braunschweig, Germany); and natural killer (NK)/T-cell lines (NL) (KHYG-1, SNK6, YT) [46-48]. Cell lines were maintained in RPMI1640 or Dulbecco's Modified Eagle's Medium supplemented with 10% fetal calf serum (FBS) (Invitrogen, Paisley, Scotland) and 1% streptomycin/penicillin at 37°C in 5% CO2. HL cell lines were treated with 5 μM of 5-aza-2′-deoxycytidine (Aza) (Sigma) for 3 days, or further treated with 100 nmol/L trichostatin A (Cayman Chemical Co., Ann Arbor, MI, USA) for additional ~16 h as described previously [15,49], and L428 and KM-H2 cell lines were treated with Aza for 6 days.
Normal peripheral blood mononuclear cells (PBMC), lymph node samples, different types of lymphoma samples, as well as sera from healthy individuals and HL patients were collected as previously described, and the reliability and quality of all the studied samples in this study have been confirmed before [41,47,50]. The study was approved by Johns Hopkins Medicine Institutional Review Board. Normal adult tissue RNA samples were purchased commercially (Stratagene, La Jolla, CA, USA or Millipore-Chemicon, Billerica, MA, USA). DNA and RNA were extracted from lymphoma cell lines and primary lymphomas using TRIzol reagent (Invitrogen) as previously described [51,52].
Semi-quantitative reverse transcription (RT)-PCR
RT-PCR was performed as previously described [40,41]. Primers used for RT-PCR are: DLEC1A: 5′-ttcctccctcgcctactc, DLEC1B: 5′-aaactcatccagccgctg; GAPDH33: 5′-gatgaccttgcccacagcct, GAPDH55: 5′-atctctgccccctctgctga. RT-PCR was done with 32 cycles for DLEC1 and 23 cycles for GAPDH.
Bisulfite treatment and promoter methylation analysis
Bisulfite modification of DNA, methylation-specific PCR (MSP) and bisulfite genomic sequencing (BGS) were performed as described [53,54]. MSP primers for DLEC1 were: DLEC1m1: 5′-gtttcgtagttcggtttcgtc; DLEC1m2: 5′-cgaaatatcttaaatacgcaacg; DLEC1u1: 5′-tagttttgtagtttggttttgtt; DLEC1u2: 5′-acaaaatatcttaaatacacaaca. For BGS, bisulfite-treated DNA was amplified using primers DLEC1BGS1: 5′-gaagatataaatgtttataatgatt; DLEC1BGS4: 5′-aactacaaccccaaatcctaa. ANKRD30Am1: 5′-cggtagttgttatttgtacgc; ANKRD30Am2: 5′-tcctctctcaataaaatcgcg. MSP and BGS were performed for 40 cycles by using the AmpliTaq-Gold DNA polymerase (Applied Biosystems). All primer sets were previously tested for not amplifying any unbisulfited DNA. Amplified BGS products were cloned and 5 to 10 colonies were randomly chosen for sequencing.
DLEC1 was downregulated by promoter CpG methylation in lymphoma cell lines
We first examined the expression of DLEC1 mRNA in 25 non-Hodgkin and Hodgkin lymphoma cell lines by RT-PCR. DLEC1 was highly expressed in the normal lymph node, PBMC samples, as well as human adult testis and bone marrow tissues (Figure 1A), but silenced or reduced in 33% (2/6) DLBCL, 50% (1/2) PTCL, 100% (5/5) BL, 86% (6/7) HL, and 67% (2/3) NL cell lines (Figure 1B), indicating that DLEC1 is a candidate TSG for lymphomas.
Figure 1. DLEC1 is downregulated in lymphoma cell lines.A. Expression analysis of DLEC1 in normal adult tissues, lymph nodes, and PBMC samples. B. Representative analyses of DLEC1 expression by RT-PCR in lymphoma cell lines. GAPDH was used as internal control. B.M: bone marrow; DLBCL: diffuse large B-cell lymphoma; PTCL: peripheral T cell lymphoma; BL: Burkitt lymphoma; HL: Hodgkin lymphoma; NL: nasal NK/T-cell lymphoma.
The promoter and exon 1 region of DLEC1 is a typical CpG island, with a T/C SNP site at CpG#29 (Figure 2A). MSP analysis showed that DLEC1 promoter methylation was frequently detected in 3/6 DLBCL, 1/2 PTCL, 5/5 BL, 7/7 (one weak) HL, and 2/3 NL cell lines (Figure 2B; Table 1), well correlated with its silencing or reduction. DLEC1 methylation was further verified in detail by BGS analysis of 61 CpG sites within the CpG island. CpG sites of DLEC1 examined were heavily methylated in BL cell lines Rael, Raji, and in the HL cell line L428, but rarely in all normal PBMC samples, which confirmed the MSP data (Figure 2C). These results indicate that DLEC1 silencing by promoter methylation is a critical event in lymphomagenesis.
Figure 2. Promoter CpG methylation of DLEC1 in lymphoma cell lines.A. Diagram shows the DLEC1 CpG island, exon 1 (indicated with a rectangle), and CpG sites (short vertical lines). Numbers from 1 to 61 indicate the individual CpG sites. B.DLEC1 methylation as measured by MSP in lymphoma cell lines. M: methylated; U: unmethylated; DLBCL: diffuse large B-cell lymphoma; BL: Burkitt lymphoma; HL: Hodgkin lymphoma; NL: nasal NK/T-cell lymphoma. C. High resolution mapping of the methylation status of the DLEC1 promoter by BGS in normal PBMCs, and representative BL and HL cell lines. The DLEC1 transcription start site is shown as bent arrows. Circles, CpG sites analyzed; row of circles, an individual promoter allele that was cloned, randomly selected, and sequenced; filled circle, methylated CpG site; open circle, unmethylated CpG site.
Table 1. Frequencies of DLEC1 methylation in various lymphoma tissues
Silencing of DLEC1 could be reversed by pharmacologic demethylation
To further confirm whether promoter methylation mediates the loss of DLEC1 expression in lymphoma, lymphoma cell lines with methylated and reduced DLEC1 were treated with the DNA methylation inhibitor, Aza, alone or combined with the HDAC inhibitor trichostatin A (TSA). After Aza treatment, DLEC1 expression was significantly induced in cell lines Rael, L428, L1236 and KM-H2. MSP analysis showed increased unmethylated alleles. Similar results were obtained using a combination treatment of Aza and TSA (Figure 3). Demethylation of the DLEC1 promoter was further confirmed by BGS. These results confirmed that promoter CpG methylation directly mediates DLEC1 silencing in lymphomas.
Figure 3. Pharmacologic demethylation restored DLEC1 expression in methylated/silenced lymphoma cell lines.A. Expression and methylation analyses of DLEC1 in HL cell lines with Aza treatment or combined with TSA (A+T). M: methylated; U: unmethylated. B. BGS analysis of DLEC1 methylation in lymphoma cell line KM-H2 before and after 5 μM Aza treatment.
DLEC1 is frequently methylated in primary lymphomas
We next investigated DLEC1 methylation in different types of primary lymphomas. Of 58 lymphoma tissues, DLEC1 methylation was detected in 5/6 (83%) BL, 16/30 (53%, one weak) HL, 1/10 (10%) DLBCL, 6/8 (75%) NL, and 4/4 (100%, two weak) follicular lymphoma (FL) tumor samples, while no methylation was detected in normal lymph node samples (Figure 4A and B; Table 1). Unmethylated bands were detected in all samples due to the inevitable inclusion of non-tumor cells in the analysis. Furthermore, the reduction or silencing of DLEC1 expression was observed in 6 out of 7 (86%) NL tumors as measured by RT-PCR.
Figure 4. DLEC1 is frequently methylated in lymphoma tissues. A. MSP analysis of DLEC1 methylation in normal lymph node samples. Templates used for positive controls are: bisulfite treated DNA from HCT116 (+ve-control1) and HT-29 (+ve-control2) cell lines for methylation; SW480 (+ve-control1) and SNU16 (+ve-control2) cell lines for unmethylation. B. Representative analyses of DLEC1 methylation in primary lymphomas by MSP in primary lymphoma BL, DLBCL, HL and FL samples. BL: Burkitt lymphoma; DLBCL: diffuse large B-cell lymphoma; HL: Hodgkin lymphoma; NL: nasal NK/T-cell lymphoma; FL: follicular lymphoma. C. Representative analysis of DLEC1 expression in NL tumors. GAPDH was used as an internal control. D. Representative MSP analysis of DLEC1 methylation in serum samples from HL patients and normal controls. Positive controls: bisulfite treated DNA from HCT116 (+ve-control1) and HT-29 (+ve-control2) cell lines. M: methylated; NS: non-specific band.
Furthermore as a pilot study, we examined DLEC1 methylation in serum samples of 13 HL patients with sera from 20 healthy individuals as controls. ANKRD30A is a normally methylated gene, thus used as an internal control for validating genomic DNA integrity of these samples, in addition to unmethylated DLEC1. Results showed that DLEC1 methylation was detected in 3/13 (23%) sera from HL patients (Figure 4D) but not in any normal sera.
DLEC1 is located in the commonly deleted region 3p22-21.3 [3,18]. A cluster of TSGs, including RASSF1A, BLU, and CACNA2D2, has been identified within this region. Silencing of RASSF1A, BLU, as well as DLEC1 by promoter CpG methylation had been extensively identified in multiple human cancers. Notably, promoter methylation of several 3p22-21.3 TSGs, such as RASSF1A and BLU, has also been identified in lymphomas [50,55], suggesting that TSGs in this region are frequently susceptible to epigenetic disruption during lymphoma pathogenesis.
Here, we report that DLEC1 is frequently silenced by promoter methylation both in lymphoma cell lines and primary lymphomas, but seldom in normal lymph nodes and PBMCs. Pharmacologic demethylation could reactivate DLEC1 expression, suggesting that epigenetic mechanism including DNA methylation and histone modification mediates DLEC1 transcriptional silencing. As primary tumor tissues usually contain infiltrating non-malignant cells such as lymphocytes, unmethylated alleles were detected in all the tumor samples which also acting as an internal control for genomic DNA integrity. DLEC1 unmethylated alleles and ANKRD30A methylated alleles detected in different types of normal and lymphoma samples indicated the reliability of these samples as shown before. DLEC1 methylation showed a relatively low frequency in DLBCL samples, compared to other lymphoma types. The reason for this difference is not clear probably due to the special biologic features of DLBCL, which needs to be further confirmed by large sample size. Our results suggest that epigenetic silencing of DLEC1 is important for lymphoma pathogenesis. Remarkably, DLEC1 is specifically methylated in sera of HL patients, suggesting its potential as an epigenetic biomarker for the non-invasive diagnosis of lymphomas.
This study identifies the frequent epigenetic inactivation of DLEC1 in various lymphomas and demonstrates its potential as a non-invasive tumor marker for the detection of lymphomas. More molecular studies on the tumor suppressive functions of DLEC1 in lymphoma pathogenesis are needed.
Competing interests
The authors declare no conflict of interest.
Authors' contributions
ZW and LL analyzed data and drafted the manuscript. XS and ZW acquired data. ZG, GS, PGM and AR provided material and reviewed the manuscript. PGM contributed to the writing of the manuscript. QT conceived and supervised the study, analyzed data and finalized the manuscript. All authors read and approved the final manuscript.
This study was supported by National Natural Science Foundation of China (# 81071634 and 81172582), Shenzhen Science Fund for Distinguished Young Scholars (#JC201005270328A) the Leukaemia Lymphoma Research of the United Kingdom and the grants from The Chinese University of Hong Kong. We thank Dr. Riccardo Dalla-Favera for the DLBCL cell lines, Drs. Teresa Marafioti and (David Y Mason) for the L1236 cell line, Drs. Norio Shimizu for the SNK-6 lymphoma cell line.
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Tao Q, Robertson KD, Manns A, Hildesheim A, Ambinder RF: The Epstein-Barr virus major latent promoter Qp is constitutively active, hypomethylated, and methylation sensitive.
J Virol 1998, 72:7075-7083. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Tao Q, Swinnen LJ, Yang J, Srivastava G, Robertson KD, Ambinder RF: Methylation status of the Epstein-Barr virus major latent promoter C in iatrogenic B cell lymphoproliferative disease. Application of PCR-based analysis.
Am J Pathol 1999, 155:619-625. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Toujani S, Dessen P, Ithzar N, Danglot G, Richon C, Vassetzky Y, Robert T, Lazar V, Bosq J, Da Costa L, et al.: High resolution genome-wide analysis of chromosomal alterations in Burkitt's lymphoma.
PLoS One 2009, 4:e7089. PubMed Abstract | Publisher Full Text | PubMed Central Full Text


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Monday, September 19, 2005
Queen meets members from Forum of Young Arab Leaders, underlines youth's role in region's development
(Office of Her Majesty Queen Rania Al-Abdullah-Press Department)Her Majesty Queen Rania Al-Abdullah, on Saturday, met with a group of men and women from the Forum of Young Arab Leaders (YAL), a network of Arab leaders aiming to contribute to the prosperity of the Arab world by creating the necessary environment for future leaders to grow and develop across all sectors of Arab society.
The YAL Forum is committed to implementing an action plan that sets the strategic vision of the organization whereby the leaders undertake projects focusing on mentorship and education and produce sector specific plans. Members are also expected to participate in offering time and effort to guide rising talents, to lead effective networking, to carry out fundraising activities and to secure sponsorships.
The meeting, which took place on the sidelines of the Clinton Global Initiative, which convened on Thursday, included an overview of the organization's accomplishments thus far.
During the event, Queen Rania, who is a firm believer in the need to involve youth in strategic discussions pertaining to the development of their communities, commended the young leaders and stressed that the next 15 years have immediate, urgent relevance – in all our roles, as business leaders, politicians, public figures, or perhaps as parents.
She added that we all have a moral responsibility and obligation towards the making of the future.
As the head of the Young Global Leaders (YGL) Nomination Committee, and the only Arab member of the World Economic Forum Foundation Board, Queen Rania also encouraged the Arab Leaders to work together towards the realization of their goals, irrespective of their individual differences, given that they come from around the world, and each have different agendas, as only then can they achieve what they have set out to do.
The YGL is a newly-formed, unique, multi-stakeholder community of exceptional young leaders, aged 40 years or younger, who share a commitment to shaping the global future. Queen Rania attended the YGL Inaugural Summit in Zermatt, Switzerland in June of this year.

subset: commoncrawl


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by November 19, 2015
What better way to bring in the New Year than to look back and see what we've learned in the past four? Here's a #TBT tribute to the posts that were a hit in 2012, plus some added questions for you to consider. Read on and explore how things have changed for your business over the years. Customer...read more
by October 17, 2014
Survey Innovation Series Part 1 It used to be that paper and postcard surveys were the way to go for gathering event feedback. The challenge with this approach? Receiving attendee feedback after the fact when the excitement has worn down and specific details are faded from memory. These days, thanks...read more
by October 14, 2014
Our faithful friend, the Cvent Web Surveys blog, turned six this month. So what better way to wish it happy birthday than to look back at six of the best posts of the year. Enjoy these reads and feel free to comment below to let us know what you'd like to see/read about in our blog for this coming...read more
by September 19, 2014
Tinder, the prolific online dating app that found its way onto the phones of millions of Americans searching for romance in one form or another, has revolutionized the dating scene for better or worse. And it's really a simple concept. The app integrates with Facebook, pulling in users' Facebook...read more
by August 18, 2014
Inter-generational differences between Baby Boomers, Generation X, and Generation Y are especially pronounced in the workplace. It's not hard to see why. The sheer range of communications styles, skill sets, aspirations, and values of different employees sitting side-by-side, confined within the...read more
by October 8, 2013
Every year, Forrester receives nominations for their Voice of the Customer Awards. As part of the process, the nominees answer a series of six questions that are designed ot get to the heart of their programs. Adele Sage shared the ten best pieces of VoC wisdom submitted by nominees based on answers...read more
by July 31, 2013
The Net Promoter Score (NPS) continues to receive a large volume of press coverage in the business and financial media. Originally, it was touted as the one number businesses needed to grow. However, time has not bared that out. Brand managers that focus solely on NPS often receive a wake-up call...read more
by April 17, 2013
Survey research can be used to assess the attitudes and opinions of virtually any group or sub-group. Most of my work has been in the areas of consumer and B2B market research, but from time to time my focus has shifted toward surveying employees. This makes perfect sense as our employees are the...read more
by October 18, 2012
Building on a previous entry about exceeding market expectations, this post will look at a particular type of survey, the planning survey. If you are in B2B market research you are likely familiar with the requests of internal clients who need to budget and schedule for the coming year. The timing...read more
by September 18, 2012
Hopefully by now we're all aware of how important social media can be to market a product or idea to a large audience. But did you also know how much social media can affect the research process? A few weeks ago The Guardian posted a great piece about how social media can be utilized during all...read more


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Tom Izzo and Mike Krzyzewski listen to a referee during the 2005 NCAA tournament game in Austin, Texas. / DAVID P. GILKEY/DFP
Chris Vannini is the managing editor of the Spartans blog The Only Colors. His opinions do not necessarily reflect those of the Detroit Free Press nor its writers. Read his column every Monday at here and follow Vannini on Twitter at @ChrisVannini.
Tom Izzo is a coaching legend.
His Final Fours, national championship, record wins at MSU and in March speak for themselves. Hes beloved by other coaches, media and fans. The Basketball Hall of Fame is waiting.
He turned MSU into a national power and always proclaimed that the program is bigger than him. But theres been one man that has almost always gotten past him. One more synonymous with a program than Izzo.
Mike Krzyzewski.
Izzo is 1-6 all-time against Coach K. Izzo has lost recruits to the Duke head coach, most recently Chicagos Jabari Parker, on whom Izzo went all in. Coach K has more Final Fours, national championships and the most wins of any mens college basketball coach, breaking the record with a win over Izzo.
Krzyzewski has two gold Olympic gold medals. Izzo has made it clear coaching the Olympic team would be a dream.
Izzo is a legend, but Krzyzewski is the greatest of all-time.
Izzo has been known more as Mr. March, and he has a respectable 1-1 record against Duke in the NCAA tournament. The loss came in the 1999 Final Four Izzos first one. Izzo got his revenge in 2005, when the No. 5 seed Spartans beat No. 1 seed Duke in the Sweet 16. The Spartans would reach the Final Four that year.
Who knows how much time either coach has left. They may never meet again in the NCAA tournament after Fridays tilt in Indianapolis.
Izzo wont surpass Coach Ks wins record, nor most of his accomplishments. But dont think it wouldnt give Izzo the utmost satisfaction to have a winning tournament record against Krzyzewski.
There is a tremendous amount of respect between the two. Izzo has admitted he has learned much from Coach K, but at some point, you want to beat him. You want the national respect and reputation Duke has. Who wouldnt?
Izzo is proud of the fact that MSUs 16-year active streak of reaching the NCAA tournament is third in the nation. Of course, its two years behind Dukes active streak.
There's no question, if you look at the NCAA tournament and what's been done, nobody's Duke, because Duke is Duke, Izzo said Monday. We are one of the closest things to it as far as consistency, and that should make for an incredible game.
No one can be Duke, but Izzo has an opportunity to finally have a leg-up on an icon when things matter the most.
Thatd be quite the feather in Izzo's cap.


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Local Catholics will have opportunities to pray with their new bishop during installation services being held in March in Harrisburg.
The Most Rev. Ronald W. Gainer, 66, will be installed as the 11th bishop of the Roman Catholic Diocese of Harrisburg during a two-service process, the diocese announced.
The Solemn Vespers service is at 7 p.m. Tuesday, March 18, at Holy Name of Jesus Church, 6150 Allentown Blvd., Harrisburg.
A Mass of Installation will be held at 2 p.m. Wednesday, March 19, at St. Patrick Cathedral, 212 State St., Harrisburg.
Service details:The Solemn Vespers service is open to the public and includes the singing of hymns, psalms, Bible readings and prayers. The service will be streamed live on the diocesan website at www.hgdiocese.org.
The Mass of Installation will be led by Archbishop Charles J. Chaput of Philadelphia. Representatives from local parishes and ministries will be invited to attend the event.
The diocese is still developing plans for live television, radio and web broadcast.
New leader:Gainer fills a leadership role left vacant by Bishop Joseph P. McFadden, who died May 2.
The new bishop will oversee 250,000 Catholics throughout York and 14 other counties.
Gainer was born in Pottsville, Schuylkill County, and ordained a priest in 1973 in Allentown, Lehigh County. Most recently, Gainer served as bishop of Lexington, Ky.
Gainer has served in parish, campus ministry, marriages and family, and tribunal positions.
-Reach Eyana Adah McMillan at email@example.com.


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The D-Link DIR-827 is a very good true dual-band router that needs a firmware upgrade so it can live up to its potential.
The top routers that support the new 802.11ac standard on the market. Since there are not many of them, all of those on this list are the best of their kind.
D-Link seems to be channeling Belkin WeMo with its new $120 Wi-Fi camera and optional bundled accessories.
The good-looking D-Link HD Media Router 1000 DIR-657 is a very good router, though it doesn't offer much over the company's previous model, the DIR-655.
Though not perfect, the D-Link DIR-857 HD Media Router 3000 makes a great router for homes thanks to its top-notch performance on the 5GHz band and a nice set of features.
The unique-looking and compact D-Link Amplifi Whole Home Router 1000 (DIR-645) makes a very good investment for homes that need a fast wired network and good Wi-Fi signal coverage.
D-Link announces the availability of its new routers and Powerline adapters in its new Amplifi family of networking products.
Browse our list of cameras to see if do-it-yourself home security is right for you.
Here's everything you need to know before taking the plunge into DIY home security.
Better placement means better protection.
The D-Link DIR-827 is a very good true dual-band router that needs a firmware upgrade so it can live up to its potential.


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Professional Development Advisory Committee
This committee will manage and supervise the Faculty/Staff Development Funds and Return-to-School Funds for Central Piedmont Community College to offer financial support to all employees interested in upgrading professional skills by attending workshops, conferences, or college classes.
Guidelines for Allocation of Faculty/Staff Development Funds
- Funding is available to all CPCC employees subject to availability of funds.
- Funding requests that directly relate to recertification or reaccreditation of a program, department, or individual will be given priority. All other funding requests must be directly work-related and mission critical to the College.
- Travel must occur within the current fiscal year.
- First time applicants, those who are presenting, members of a panel, or an officer of the sponsoring organization will be given priority.
- Employees may request a maximum of one (1) out-of-state trip and one (1) in-state trip per fiscal year.
- A completed application with required signatures, a paragraph explaining how this training benefits the College, a program brochure, and a Request-for-Authorization-to-Travel form must be submitted to the committee for approval prior to attending the conference.
- Funding awards cover transportation, lodging, meals, and registration.
- Upon return from funded travel or other professional development activities, attendees are expected to "give back to the College" in some way. Presenting conference information to your department, unit, or Learning College Showcase are examples of ways to "give back".
- Individuals who submit their applications for funding after attending an activity are not guaranteed an award.
- A call for applications will be done twice a year-contingent on funding.
- The maximum amount the will be awarded will be set at a $1,500.00 limit per trip. Any amount over $1,500.00 will be the responsibility of the traveler. Additional funding sources may be utilized.
- When there is more than one individual from a department requesting travel funds, the department may be awarded up to $3,000. The department may allocate this amount among department members to get the maximum benefit.
- Maximum benefits received over a three fiscal year time period will be set at $3,000.00. A fiscal year is July 1 through June 30.
- Funding to attend the same conference in consecutive years is not guaranteed.
- Upon reaching the maximum dollar amount an applicant must wait one fiscal year before re-applying for funds.
- All requests for reimbursements must be submitted to the Center for Leadership and Staff Development within 10 days of return to campus.
Guidelines for Allocation of Return to School Funds
- Funding is available to all CPCC employees subject to availability of funds.
- A completed application with appropriate signatures and a paragraph describing the benefits of this training to the College must be submitted to the committee for approval prior to beginning the semester.
- First time applicants are given priority.
- An applicant may be fully or partially funded based on the maximum allowance of $700.00 per semester not to exceed $2,100.00 over three semesters.
- Upon reaching the maximum dollar amount--$2,100.00--an applicant must wait one fiscal year before re-applying for funds.
- Schedule of application submittals:
- Summer Semester-Due by April 30
- Fall Semester-Due by August 30
- Spring Semester-Due by December 30
- All requests for reimbursement must be submitted in a timely manner to the Center for Leadership and Staff Development.
Committee Membership
- Chairperson
- Must have served on the committee at least one fiscal year prior to becoming chair
- Preside over and moderate all Professional Development Advisory Committee meetings.
- Serve a term of two fiscal years.
- Vice-Chairperson
- Serve a term of one fiscal year before assuming the position of vice-chair.
- Moderate the Professional Development Advisory Committee monthly meeting in the absence of the chairperson.
- Secretary
- Provide an up-to-date balance of both funds.
- Take the minutes at each meeting and make them available from prior meeting for approval.
- Members
- Fifteen members equally divided among Faculty, Administration, Professional and Classified Staff make up this committee.
- Committee members are to serve a term of three fiscal years.
- Upon completion of term the member will elect to remain on the committee or rotate off and be replaced by a member of the same classification.
- Decision Making
- A quorum must be present for decisions to be made.
- If a quorum is not met, the voting will take place via e-mail the next business day.
- If a member misses either 2 consecutive meetings or 3 total meetings during a fiscal year without advanced notice, the secretary will contact him or her with regard to their further service on this committee.
- If a committee member has an application involving Faculty/Staff Upgrade Allotment or Return-to-School funds up for review, that committee member will be asked to excuse himself from the discussion and vote of the aforesaid application.
- Absenteeism
- Any member who must miss a meeting should notify the secretary ahead of time.
- Missing either 2 consecutive meetings or 3 total meetings during a fiscal year without advanced notice, should be contacted as to his/her intentions about remaining on the committee.
- If a member is unable to serve the required three fiscal year term, a replacement shall serve to the end of the member's term and can be eligible to serve an additional three-year term.
Committee meetings will be held once a month unless Faculty/Staff Development and Return-to-School funds are depleted.
Changes to the Bylaws
The Bylaws will be reviewed and amended if necessary at the beginning of each fiscal
Committee Members 2012-2013
- Joann Alexander, Executive Administrative Assistant, Retention Services, Chair
- Teresa Caesar, Academic Advisor, Advising and Student Success Centers
- Saronda Easter, Director Campus Administration, Cato Campus
- Angela Eddie, Administrative Assistant III, Compliance and Audit
- Catherine Flores, Program Chair, Medical Assisting
- Fay Foster, Special Events Coordinator, Foundation/Institutional Advancement
- Erin Payton, Librarian Senior, Library Services
- Mona Rabon, Director, Leadership, Management & Professional Development (Corporate and Contract Training) and Teacher Education
- Elena Randolph, Executive Director, Budgets and Payroll
- Davandra Reed, Instructional Office Assistant, Mathematics
- Rowell, Ann, Instructor, Accounting and Business
- Brenda Sanner, Instructor, Health Sciences
- Diann Back, Director, Center for Leadership and Staff Development, Ex-officio Member


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Trevecca women's soccer coach Brett Armstrong has resigned after the most successful season in program history.
Last fall the Trojans went 16-5-3, claimed two tournament championships, the first Great Midwest Athletic Conference regular season and tournament titles and a fourth-place finish in the NCCAA championship.
The team also set program records for wins, win streak (eight), shutouts (15) and consecutive shutouts (six). ...


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- KNIA / KRLS - http://kniakrls.com -
Summer Iowa Games Deadlines Near
Posted By News On June 17, 2010 @ 5:58 am In Today's Local Sports | Comments Disabled
Residents in the Marion County area that are considering competing in this year's Summer Iowa Games are running short on time to register for many of the game's events. Registrations for many of the sports must be submitted by Monday, and some deadlines have already passed.
Last summer, 226 residents from Marion County participated in the games, and organizers are expected over 15,000 athletes from around the state to take part this year. More information on the 2010 Summer Iowa Games, including how to register, are available at www.IowaGames.org [1].
Article printed from KNIA / KRLS: http://kniakrls.com
URL to article: http://kniakrls.com/2010/06/summer-iowa-games-deadlines-near/
URLs in this post:
[1] www.IowaGames.org: http://www.IowaGames.org
Click here to print.
Copyright © 2009 KNIA/KRLS. All rights reserved.


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Four alumni were inducted into the Shawnee State Hall of Fame on Jan. 18 at the annual banquet. From left, are, Dan Grummitt (1996-98), baseball player drafted by the Tampa Bay Devil Rays and the only SSU player on an MLB affiliated roster, reaching Class AAA level in 2003; Becky Holden-Elkins, (1997-2002), the only student-athlete to play five different varsity sports and holds the career record in women's basketball for free-throw percentage (81.2 percent); Sarah Koehler Downing (2004-07), the only women's soccer player in program history to score 10 or more goals in every season; and Zanesville's Adam Davenport (2001-05), who ranks fifth all-time with 110 starts in 126 games and a two-time second-team All-American Mideast Conference honoree.
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WELCOME TO THE DAILIES THREAD!Anyone and everyone can post your runs, cross-training, abdominal work, rest days, race plans, training questions, etc. Hopefully the Dailies will be a terrific source of motivation for runners and serve as outstanding accountability. This group can be highly supportive of all life experiences. So, come on in, put your feet up and stay awhile, but don't forget to run, too. And whoever is up first is welcome to start the thread.I suggest the above paragraph be copied and posted as the start for every Dailies thread, then whoever starts the thread would post the first response.
An "epic" journey: From Miler to Ultra Marathoner Blogs
12/28/2015 - Across the Years 24-Hour, Phoenix, AZ
Kinda mumbling and stumbling through my morning routine. We had some great races from forum members over the weekend. Me? I went shopping and bought two pairs of shoes. With the mileage I'm running I'm going through shoes at an alarming rate. I was looking for something similar to the Saucony Ride 6 but with more cushioning to help alleviate the impact and pounding on long runs. I ended up with a shoe from my favorite shoe company, Asics, the Cumulus 16. Since I was at the LRS anyway I decided to save myself a trip in another month or so and picked up replacements for the Ride 6 with the successor, the Ride 7. I still want then for shorter runs, tempo runs, and intervals. In all the years I've been running, this is the first time I've had shoes for different types of workouts.
Hey George,
There are worst ways to spend a weekend!! LOL
Zumba tonight gang. Weight bearing. Fighting a chest thing. Hopefully it's just allergies and goes bye bye soon.
PR 2013 25:58 (only 35 seconds off from placing in my AG) Yahoo!!!!
Don't go to your grave with your treasure still inside you
:::the march is on:::
Winter racing series:
George I would love to hear how you like the Ride 7's. I currently use the Ride 6 and am curious if the 7's will be the same or if they changed a lot of things
wcrunner - A new pair of shoes makes any day a good one!
Lori - You will be in the 23s when you clear up!! My allergies have also been acting up through this last week. I take an allegra-d daily but I still feel like I gag and spit for every first mile of the morning. Hope you feel better..
My legs still feel a little trashed this morning but Ive only got a 2 mile run planned for today. Ill have to wait til after work but luckily its still 61 degrees and wet here in WV..
Hope everyone has a good day..
Hey, there are worst ways to spend a weekend George. I wounder how the Cumulus compares to the Nimbus. I've been running in Nimbus but the Cumulus is a bit cheaper, lol. It's really hard for me with shoes, there is no store near me so I order off line.
BA30 and Melissa, hope you are feeling better!
I did 2 miles this morning. The short runs are killers, I don't seem to hit my stride until somewhere in mile 2 and then I'm done. I do walk for 5+ mins to start and try to take it easy going out. I also think I need to plan my routes a bit better. Hills in that first mile are enough to make me want to quit. Wish there were more flatter areas to run, don't know who's idea it was to stick the darn town on a hill.
Lori - You will be in the 23s when you clear up!!
Thanks Mike--can I call you Mike? I'll start with 25:00 flat first.
Our local 5k course has about .75 miles then a monster hill, then flat bike path, then hill down. Honestly that hill at the beginning is a bastardly obstacle but I think it would be much worse at the end.
I still have nightmares of my epic crash and burn at the NYS HS X/C Championships which had a 1/4 mile hill to the finish. Horrible!!
Feel free
Mike mike mike mike
Are you freakin' kidding me?? Lori would have totally blown away the sub 25:00 group spreadsheet had she not been injured for 6 weeks prior to her race and then sick on the day of the race! You are one tough runner, Lori!
Lynn2015 PRs... 5K (23:58) /10K (53:45) /15K (1:21:50) /Half (2:06:32)
Get Lucky! Half Marathon: Chicago, IL... 03/12/2016
GO! St. Louis Half Marathon: St. Louis, MO... 04/09/2016
IL Marathon Half Marathon: Champaign, IL... 04/30/2016
Chicago's Spring Half Marathon: Chicago, IL... 05/22/2016 (if needed)
Rock Hall Half Marathon: Cleveland, OH... 08/14/2016
Runner's World Festival Hat Trick 5K/10K/HM: Bethlehem, PA... 10/14-16/2016
Rest day for me today... and back to work tonight. Fun Run, speedwork and trails coming up this week, so I'd better rest when I can and be ready to rock.
Thanks Lynn! I have to say two things---having fresh legs was a good thing BUT, I still have this sneaky suspicion that the course was under mileage. My Lifetrak has never been off by being under. I have no explanation for it. Some of the runners were using the race for points??? So I assume someone else will complain. For now I'll take the time as I know it was the best I could do on that day. First time I was totally relaxed and breathing comfortably the 1st mile.I'll take it!
25:00 seems not so far away now........
You know... it is a funny thing how the courses at races don't always seem to be the same! I had always assumed that there was a standardization process in place and there was no variability allowed. Melissa and I were just saying on Saturday that we thought the course we ran seemed a bit long according to our portable devices. Who knows??! And in fact... I was at the airport today for an issue with my airplane and on a whim decided to take a look at the course for my next race to scope it out and learn the turns and it came up short by my measurement and I drove it twice! Maybe these things all average out in the end... at our level, it's not that important to be down to the yards and feet. It's all fun!
And congrats on your new PR!! I have always thought that the first person who would break 25:00 from our forum group would be you.
I still have several hundred miles left on the Ride 6's so it may be 4-6 weeks before I start using the Ride 7's. They didn't feel different trying them on in the store but that can change when you're 5 miles into a run.
Here's a couple links with the specs so you can see the differences:
Nimbus 16
Cumulus 16
In a brief glance, the Cumulus has a 10mm drop while the Nimbus has an 11mm drop and is slightly heavier. since the description specifically mentions both mid and heel gel cushioning for the Nimbus, but doesn't for the Cumulus, I'm assuming the Cumulus lacks the mid-foot gel cushion. Overall the Nimbus sounds a little bulkier and cushier.
Just got in my 2 mile run. I left the watch at home today. I felt really good and am sure I was running a faster pace on mile one than Ive been running plus my breathing was good but mile 2 was a different story.. I had to slow down and my body wanted to walk. I have to keep reminding myself, it took 4 years for me to get in this shape and Im not going to get back overnight..
On course measuring: Im not sure how everyone measures a course but I remember the course of my first 5K. I was running well and had a goal time of around 27:00. I ran what felt like a great race and pushed myself very hard only to see a 29:30 posted.. I was discouraged. Later I came to find out it was a 3.45 mile course!
My wife and I direct a small 5k for a local Cancer foundation (Julia Price Cancer Foundation Spooktacular 5K) and when we first put the race together, I used google earth to lay out the course.. It was somewhat tricky but Im almost certain that its 3.1 exact.
Thanks George.
True, hills at the end suck too!They just released the map for my local 5K fun run which will be my first 'race'. It's really hilly, Including a hill to the finish line, eek!
Had the day off and wasn't sure if I was going to go out after 8.6 miles yesterday but took a nap and went out around 6:30. It was really humid and 80, but it looked like it was going to rain but I thought running in the rain might be fun. Just kind of lollygagged around at the battlefield...wasn't really pushing anything today and ran 2.9 miles. I have this weird thing going on with I believe my peroneus tendon on my right leg...right now I'm just rolling it and not pushing it. I'm trying to slowly ease into this hot weather. Last summer was difficult but I also learned alot. Right after I finished running the sky just opened up....it was a nice cool way to end a great day off!
Next Up:Wounded Warrior 5K, Farmington NM, Nov 8, 2014
Turkey Trot 5K, Farmington NM, Nov. 22, 2014Reindeer Romp 5 K, Farmington NM, Dec. 20, 2014
Nice job, folks!
I decided that it was time to put my streak to bed today. My soleus wasn't feeling any better, and in fact it was hurting quite a bit on the stairs down to the parking lot this afternoon. Discretion being the better part of valor and so forth, I decided that I'm satisfied with my two-week streak and took the night off. I may take tomorrow night off as well, depending on how it feels. I may try to do another streak during the #RWRunStreak period, but I'm not going to hurt myself over it. 14 days was longer than I thought I could go, from both a conditioning and (even more) logistical perspective. Sometimes I push too hard to achieve something, but I'm getting better at finding the perspective to back off before I do any damage to myself.
Keep Moving Forward!
My story
PRs: 5K - 34:26 (6/21/14), 4 mi - 48:57 (3/15/14), 7K - 55:06 (2/2/14), 10K 1:13:11 (5/18/14)
Dunkin Run 10K - 9/7/14
Insane Inflatable 5K - 9/13/14
Palio 5K - 9/21
Great Pumpkin 10K - 10/18/14
Disney's Wine & Dine Half Marathon - 11/8/14
Walt Disney World Half Marathon - 1/10/15
SEFCU Foundation 5K - 9/1/14
Crossings 5K Challenge - 9/28/14
Beat Beethoven 5K - 10/5/14
Squirrelly Six-Mile - 10/26/14
Troy Turkey Trot 10K or Ellis Hospital Cardiac Classic - 11/27/14
Mark--I think that is a wise decision--I am not sure why RR promotes those streaks. For many people running back to back to back days is really setting yourself up for injury . For a running organization to promote running streaks seems odd. Hope your soleus feels better soon.
I've thought the same thing because it also contradicts advice they give elsewhere about the importance of rest and days off. At one level running one mile to keep a streak going might as well be a day off for someone running 50 mpw, but that's not the case for lower mileage runners and beginners. Streaks do appeal to runners with a certain mindset. Every once in a while when I find I've run 8-9 days in a row I think about continuing to see how long I can keep the streak going, but that usually comes about the time I need a rest day, so the "streak" ends instead.
I expressed my sentiments at the beginning but also felt like, what's the use of just running 1 mile to say you "did"? I mean, how does that help your over all running fitness? To my mind, taking a day of rest and then running your normal 5-6 is more beneficial. But to each his/her own. I know I need the rest days. Straight up.
Since I'm still relatively new to running, I'm trying all sorts of different things to see what works and doesn't work for me. I just need to try to be aware of when I need to pull the plug when something isn't working. I've got a tendency to push through without paying attention to the signs that my body is sending me. I certainly did see where it could be a good thing, but not necessarily doing it long-term or when one is still pushing hard enough due to other factors, like me.
It's especially hard, at least for me, to NOT push when the weather is gorgeous. I wish I would have had the good sense to shut it down when I first started to get the knee twinges. Lesson learned.
I'm just glad you're not hurt Mark. You've worked too hard.


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Sporeling Sprout
Looks around and idles about.
Pit-pattering footsteps; vocalizations (on-click)
Can be found in Sporeggar.
Took the first one in the middle of the "circle" and it was a rare (Y)
After being so emotionally involved in the plight of the Sporelings back in BC, it does feel a little weird to capture one of their children and send him off into a series of deathmatches, but.... OH WELL.
I actually flew to the location and there was a rare one right in the middle of an enemy faction town. Caught it right away and then died. Worth <3
Very good humanoid pet, many breeds to chose from. P/P with 325 power or S/S with 325 speed are obvious choices. If you chose power, you can take Charge and still go first. S/S has a bit more advantage with Crouch. Use Leech Seed to be even more durable. Or go with a Moonlight team and use Creeping Fungus, possible partners Nether Faiere Dragon and Infected Squirrel. All in all this is a winner, and very durable with Crouch, Humanoid racial and Leech Seed.
Can people recommend which breeds are best for this guy? I found a rare H/S, and was wondering if I ought to keep it, or continue my sporeling genocide until I find another one. >.>
First time there, there were around 10 sporelings around. I checked everyone and forfeited, last one was a rare. Wasn't there for more than 10min.
Got my rare (B/B) lvl 17 after 1 hour trying. It was the only one left \o/.
Goldrinn - 02:10pm
spent almost 3 hours out here last night, no rare, logged in today, first one i get is a rare. 17.54, just outside sporeggar. good luck. lvl 17
I came up tonight and after about 2 hrs got a rare...behind the NPC Gzhun'tt It is now 4:21 am on Zangarmarsh server. There were about enough in the Sporeggar village area but most were gray. One other person was trying. Weather was mostly dry with occasional rain during my time here. Even though I had humanoids I bought, this one was the first to count towards my achievement Family Reunion. Thanks for comments! I read up before coming out. I love this website :)
CUTE! Attacks are the best EVER, and, see my comment on the Restless Shadeling to see what pets this pet should be teamed up with.
UPDATE: Poor is the one I have! Works GREAT!
I just caught this guy 1:15 pm PST on Skullcrusher. I first arrived at 1:11pm and found none around Sporeggar. So I read the advice of the other commentors and flew around and found one battle to kill time.
I found a Sporeling Sprout exactly next to Mycah, Sporeggar's Quartermaster. I was also extremly lucky that my first spawn was a Rare!
Suffice to say, I fought'em, I caught'em and I'm glad I got'em!
*90 Panda Monk, 2737/3000 Neutral - Sporeggar
Someone said on wow head that you might have to be friendly to find these. I was unfriendly with them and I found two of them on my way out for other pets. I accidently killed one then there was another one. It was poor but glad I have one. Also, I found them on the outskirts of Sporagar.
Found a Rare standing next to the Battle Master in the Sporeling Village. He leveled up very quickly and he packs quite a punch.
I use him as my main "go-to" for dragon fights. Throw down some 'shrooms, hit 'em with a leech seed and then power hit with the jab! He was soloing mobs very early on and with the self heal I had hardly any downtime waiting for the healing timer to reset.
Was flying around Sporeggar at 12:27pm server time, read a comment that says these spawns every 15 mins, so I waited, at 12:30pm server time, a few spawned, clicked the first one and got a rare xD I was at 19.49, 54.16
I was just at Sporegar to catch my first Sprout. When I got there there were at least 6 so I figured I might be able to get a rare. Well some stinking horde with the Tamer title decided to be "nice" and went around and killed them all! I managed to get into one battle and get a common but really? Was that necessary? Seems someone who worked that hard to get that title would be a little more respectful of others trying to do the same...Alliance/Horde or not!
Stood about waiting at Sporregar and at around 3:30pm, loads spawned! My 1st was poor and the 2nd was rare. Can't wait to level him up!
I had such a difficult time finding a rare one that I had begun to wonder if others were pulling my leg. I finally logged an alt in Sporeggar. After several days of random checking, I finally managed to encounter and capture a rare Sporeling Sprout.
Was not that hard to get one, I just hung out in the area while doing some low level quests ... after hanging out for a few days, I got uncommons and my first rare yesterday! Now ready to level it up quickly!
This little guy kicks some serious tush in the battles, very powerful little fellow i love him! got my rare at about 3pm server on Proudmoore, the whole area of them respawns every 15 mins. They actually spread out a little further than just sporeggar i found mine just outside the town the area was not classified on the map as sporegar.
I got mine about 5 minutes after the hour, waited and watched and another player got a second one by 10 minutes after.


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New York, New York - Posted on: Tuesday 01/14/14
- Build and execute against the marketing plan, developed in conjunction with the CMO and Exec Team
- Manage the marketing budget and the use of the marketing budget by sales and the other teams.
- Own the success of the marketing team - manage and assess the staff
- Work across the organization to drive total company success
- Develop a strong understanding of customer needs to inform product messaging and successfully communicate the LiveIntent value proposition to publishers and advertisers
- Product launch – plan the launch of new products and releases and manage the cross-functional implementation of the plan
- Lead global cross-functional initiatives to drive the marketing strategy for go-to-market plans for product launches
- Collaborate with sales to address their needs including maintaining a robust sales kit including presentation materials, product collateral, case studies and competitive positioning
- Market intelligence – be the expert on your buyers, how they buy and their buying criteria; be the expert on your competition and LiveIntent's competitive edge
- Lead large publisher & advertiser programs, communication planning and marketing campaigns to drive platform business growth.
- develop merchandising materials and other corporate and partner collateral
- Assist with sales training on product positioning – ensure all sales members can internalize and clearly communicate our product positioning and message.
- Define, develop and execute the product marketing programs for our direct clients and verticals, strategic partners and new channels
- Work closely with Analyst firms like Forrester
- Manage day to day relationship with outside PR and Marketing agencies
Minimum Qualifications
- Proven Success creating and/or differentiating a service or technology brand in a crowded marketplace
- 5-8 years of experience in B2B technology marketing, or launching new technology brands
- BA/BS degree. In lieu of degree, additional relevant work experience
- Previous management experience leading marketing and/or sales organizations
Preferred Qualifications
- B2B and/or technology marketing experience, and experience in the email, search, mobile, and/or online display advertising industry
- Experience driving sales teams engagement and success and working closely with the senior management
- Proficiency with marketing automation tool sets, including but not limited to Salesforce.com, Hubspot, Google Analytics and other similar applications.
- Self-starter with a willingness to operate at both highly strategic and very tactical levels
- Experience launching software as a service product suites and services
- Experience building and executing strategic product marketing plans
- Ability to work in an agile and occasionally chaotic startup environment
- Highly analytical; ability to define and track the key metrics for various product marketing efforts
- Outstanding communication skills, including strong writing abilities and proven experience in making complex technical subject matter accessible to a broader audience.
- Strong track record managing go-to-market activities for new product introductions and ability to develop marketing plans within a quickly changing competitive landscape
Interested candidates please apply online at: http://hire.jobvite.com/CompanyJobs/Careers.aspx?k=Job&c=qaj9Vfwh&j=oRx8Xfwd


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"Horses and cats are good!!!" I don't know about the pacheco pass..Must be a commute??
I'll have to friend you on here to keep in touch..My hubby stays gone during the wk while working he just stay's there and comes home on the weekends..Horses are my passion in life I have to have one...I had two different types of arabs before my appy I have now..I hated my H for it but had to get rid of them..He was upset because I went off one and broke my arm..Crazzzy! Things like that happen sometimes.


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November 28, 2015
Police say this is not a theft ring. They say the cars are just easy to steal. Ten were stolen this past weekend. Police have made six arrests but none of the suspects are connected. The thefts are mainly taking place on Tuesday and Sunday. Police say the thieves are boosting the cars, and then dumping them, and moving onto to another car. They recommend an after market alarm system to protect your investment. At Nevada Auto Sound in Reno, a basic system runs $199 dollars, with the most advanced being $599.
Click Here to access the online Public Inspection File
Viewers with disabilities can get assistance accessing this station's FCC Public Inspection File by contacting the station with the information listed below. Questions or concerns relating to the accessibility of the FCC's online public file system should be directed to the FCC at 888-225-5322, 888-835-5322 (TTY), or email@example.com.


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We can offer you every activity you can think of which can make your active holiday exciting and different. Experience yourself!
Groovy attractions for kids +
relaxation for parents = A wonderful familiy holiday!
Enjoy the perfect combination of relaxing and nature and return to daily life fresh and relaxed!
date unknown


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I run trainer as administrator... Run Terraria... Load up my game... Hit escape to bring up inventory, run around a bit... Then press F1. It says trainer activated, but none of the codes work Edit: Yeah just checked Steam news. There was a big patch JUST released. How lucky am I that they literally patched the game WHILE I was downloading the trainer O_O
[Edited by Vervex, 6/2/2011 9:39:57 AM]
I hear the usual "Trainer Activated" and "Trainer cannot find game" several times, but the number keys never activate the cheats.
Any help would be appreciated.I think my cheating days are over.
[Edited by tribes1tk, 6/3/2011 12:49:40 AM]
[Edited by nx1179s, 6/3/2011 9:15:32 PM]
I don't think a new trainer is in the works. Does anyone have any ideas?


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After about 24 hours of road work, the Pennsylvania Department of Transportation has reopened southbound I-83 in southern York County.
The lanes reopened at 1:30 a.m. Sunday, according to a PennDOT news release. They had been closed since 1 a.m. Saturday because of safety concerns about the stability of the pavement, the release said.
A deteriorating drainage pipe, located in Shrewsbury Township, had created a major dip in the highway, so crews from Kinsley Construction took Saturday to repair it, according to PennDOT.
Work involved excavating the area and installing a concrete slab with steel beams encased in it to provide support for vehicles traveling over the area, the release says.
The deteriorating pipe will still need to be addressed, PennDOT says, and work will be done within the next few weeks when material for the insertion of a pipe liner becomes available.


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ResearchToday in Mobile
Nokia this morning announced that it is ousting embattled CEO Olli-Pekka Kallasvuo in favor of Stephen Elop, who will leave his post as head of Microsoft's Business Division. As Kevin at… Read more »
Nokia this morning announced that it is ousting embattled CEO Olli-Pekka Kallasvuo in favor of Stephen Elop, who will leave his post as head of Microsoft's Business Division. As Kevin at… Read more »
Hewlett-Packard has no plans to move aggressively into the smartphone business following its $1.2 billion pickup of Palm, CEO Mark Hurd said last week. That's too bad, because the pieces are… Read more »
The good news continued to roll in for Android this morning with Millennial Media's claim that ad requests from Google's mobile OS grew 77 percent from March to April and have… Read more »
Microsoft used its MIX10 event in Las Vegas to showcase its upcoming Windows Phone, and to demonstrate that the OS already has the backing of some impressive third-party developers. And the… Read more »
Google has introduced a nifty app that allows users to search contacts and other data by drawing letters on the screen of their phones. But it's available only on handsets running… Read more »
Gartner released its latest smartphone sales estimates today, and it won't surprise you in the least to learn that Android and the iPhone OS were the two fastest-growing platforms in 2009… Read more »
The developer world is abuzz with reports that Google has forked Android, using a new lock type, new security hooks and a revamped framebuffer driver infrastructure. The news marks the latest… Read more »
From a mobile developer's point of view, the iPad is a revolution waiting to happen. The gadget's 9.7-inch screen, allowing for glorious visuals and vastly superior touch navigation, simply can't be… Read more »
Google today postponed the release of two Android phones that had been slated to come to market through China Unicom. The move ups the ante in the high-profile conflict between Mountain… Read more »
LG, like many of its fellow manufacturers, is shifting its focus toward Android and away from Windows Mobile. As Microsoft scrambles to churn out the newest version of its mobile operating… Read more »


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One Cleveland teen arrested, accused of stabbing another to death
CLEVELAND - A 17-year-old is under arrested after allegedly stabbing and killing another 17-year-old.
Police said they responded Monday at 3:26 p.m. to a report of a man stabbed in the 3500 block of Marvin Avenue.
Once at the scene, they said they found a 17-year-old male with a stab wound to the leg, as well an unresponsive 17-year-old male with head trauma and multiple stab wounds outside of the residence.
The teen with leg wound was taken into custody in the homicide after an initial investigation, according to police. He was brought to MetroHealth Medical Center, where he is being treated for his injuries.
A next of kin notification has not been made at this time.


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EMA has provided a variety of logo options for members to display as frequently and prominently as possible:
Members may also display the logo on their website, use it in communications or outreach to business employees and colleagues, schools and other groups in their local communities.
Click here to download recycle logo user guide (pdf file).
Click here to view downloadable version of logo.
Click here to download standard press release to send to businesses and customers in the community.


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Sophia Gorrell swims for a YMCA of Vineland team during the summer. / Staff photo/Charles J. Olson
VINELAND — Swimmers who want to get in shape for the coming season are invited to register for competitive swim camps at the YMCA of Vineland, 1159 E. Landis Ave. The camps will be offered from Monday through Oct. 18.
Stroke Camp, for ages 6 to 12, will meet from 6 to 7 p.m. Mondays, Wednesdays and Fridays.
Conditioning Camp, for ages 11 to 14, will meet from 7 to 8:30 p.m. Mondays, Wednesdays and Fridays.
High School Returning Seals Swimmer Camp, for ages 13 to 18, will meet from 4:30 to 6 p.m. Tuesdays and Thursdays and from 7:30 to 9 p.m. Fridays.
The annual swimmers and parents meeting, for new and returning swimmers at the YMCA, will take place at 6:30 p.m. Oct. 1. Everyone must attend to learn about team and parent participation, swimsuit sizing, schedules, the team handbook and other information.
For information, call (856) 691-0030 or visit www.ccaymca.org.


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The Black Cats manager is aware the upcoming transfer window will be his "only opportunity" to make addition, in an attempt to push his side above 15th place in the Premier LeagueSunderland manager Martin O'Neill is looking to take his "only opportunity" to add to his squad in the January transfer window.
The Black Cats sit 15th in the Premier League before the Boxing Day fixtures and are one of only three teams in the division to have scored fewer than 20 goals.
"It's the only opportunity that you're going to get," O'Neill told reporters of potential additions.
|Sunderland are 13/2 with Bet365 to draw 1-1 with Manchester City
"Easier said than done, of course, like everything else, and sometimes the prices become a bit prohibitive.
"But, that taken into consideration, I think we have to be looking at it just in terms of strength in depth."


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tnx bro.. been really busy to work and I would put my post game fosho just not enough time cuz when I have free time that's when i study my picks and put it here. But do the post game as I love reading about your post here like everyone else. Im just happy that pretty much my reasoning pays out and prolly my post game will be all similar to my reasoning since what I expect is what shows on most of the games yesterday.
MIA Def again shows up and with mil on fire 1st qtr but that's about it.
BK pushing hard to score right at the beginning and when CHI realizing it they step up their def and bk barely can't score until the 4th collapse or I would say relaxed from the chi.
MEM finally did what they suppose to do since game 1 and to slow down the phase it was close as garbage baskets was big on a low spread of 178 but right from the start of the game mem was able to control the phase of the game.
Hope magic still continues tonight
Originally Posted by joshua1014: nice job jpaco.. clippers messed up my 6 team parlay push on the heat o/u
tnx bro and you'll get your parlays tonight
Copyright © 1995-


text:
|T lymphocytes in food allergy: overview of an intricate network of circulating and organ-resident cells.
MedLine Citation:
|PMID: 12144637 Owner: NLM Status: MEDLINE
|Although food hypersensitivity might be divided in IgE- and non-IgE mediated food allergy, there is a large body of evidence implicating T lymphocytes overall in the pathogenesis of food allergy. Priming of naive T cells will occur mainly in Peyer's patches (PP), where surface receptors (l-selectin, CCR7 and CXCR4) will help to initiate diapedesis of the cells to the submucosa. Various antigen-presenting cells (e.g. dendritic cells, M cells) will present food antigen-derived epitopes and initiate either non-responsiveness, or a food-mediated immune response. Food-specific memory T cells express various surface receptors such as the alpha4beta7-integrin, or the cutaneous lymphocyte antigen. It is speculated, that they might also express specific chemokine receptors (CCR4, CCR7 or CCR9). Organ-specific homing will be facilitated through the corresponding receptors (i.e. MAdCAM-1 in the gut, VCAM-1 or fibronectin in other mucosal organs, or E-selectin in the skin). Locally secreted chemokines might help to attract T cells through their corresponding chemokine receptors. Finally, potential T-cell directed therapeutic interventions (peptide-derived immunotherapy, DNA vaccination, or strategies preventing T cells from trafficking to target organs) are discussed.
|Philippe A Eigenmann
Related Documents :
|6841827 - Role of immediate food hypersensitivity in the pathogenesis of atopic dermatitis.
20642207 - Safety, tolerability, and immunologic effects of a food allergy herbal formula in food ...
20490817 - Allergic rhinitis caused by food allergies.
7943997 - Adverse food reactions in patients with grass pollen allergic respiratory disease.
20373677 - Nitric oxide scavenging by food: implications for in vivo effects of diet.
22781507 - Maintenance of changes in food intake and motivation for healthy eating among norwegian...
Publication Detail:
|Type: Journal Article; Research Support, Non-U.S. Gov't; Review
Journal Detail:
|Title: Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology Volume: 13 ISSN: 0905-6157 ISO Abbreviation: Pediatr Allergy Immunol Publication Date: 2002 Jun
Date Detail:
|Created Date: 2002-07-29 Completed Date: 2003-02-19 Revised Date: 2008-05-28
Medline Journal Info:
|Nlm Unique ID: 9106718 Medline TA: Pediatr Allergy Immunol Country: Denmark
Other Details:
|Languages: eng Pagination: 162-71 Citation Subset: IM
|Department of Pediatrics, and Division of Immunology and Allergy, University Hospital of Geneva, Geneva, Switzerland. email@example.com
Export Citation:
|APA/MLA Format Download EndNote Download BibTex
|MeSH Terms
Cell Adhesion
Chemokines / metabolism
Digestive System / immunology
Food Hypersensitivity / etiology, immunology*, therapy
Immune Tolerance
Immunologic Memory
Integrins / metabolism
Models, Immunological
Peyer's Patches / immunology
Receptors, Chemokine / metabolism
T-Lymphocytes / immunology*
Reg. No./Substance:
|0/Allergens; 0/Chemokines; 0/Integrins; 0/Receptors, Chemokine
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Clinical management of hereditary angio-oedema in children.
Next Document: Could administration of bacille Calmette-Guérin vaccination at birth protect from the development o...


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For information 410.787.4000
There may be other brand names for this medicine.
You should not use this medicine if you have had an allergic reaction to dantrolene, or if you have active liver disease (especially hepatitis or cirrhosis). This medicine should not be used to treat muscle spasms caused by rheumatic disorders.
© 2015 UM Baltimore Washington Medical Center. All rights reserved.
301 Hospital Drive, Glen Burnie, MD 21061 | 410-787-4000 | TTY 410-787-4498


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Reebok Trainex Waterproof Safety Toe Work Boots for Men
Sale price: Clearance price: Starting at: Sale starting at: Clearance starting at: Reg. price:
Keep your feet dry and comfortable on the job without sacrificing safety with the Trainex Waterproof Safety Toe Work Boots for Men from Reebok®. The Trainex Waterproof Safety Toe combines a tough, water resistant, crazy horse leather upper with a smooth nylon lining and waterproof membrane for breathable, 100% waterproof protection. This boot also incorporates a lightweight, metal detector-friendly ASTM 2413-rated, composite safety toe for superior worksite protection. Under your feet, The Trainex 6'' Waterproof features a removable EVA cushion insole insert with sponge rubber heel wedge over a PU cushion midsole with Aircom heel airbag to cushion and support your foot on every step.The boot's dual density rubber outsole sports a work-friendly 90º heel, oil-, abrasion-, and chemical-resistant rubber, and a slip-resistant tread pattern. PU Direct Attach Construction locks this high performance outsole to the boot, giving it a tough watertight bond, less weight, and greater flexibility. Airline- and metal detector-friendly 100% non-metallic construction. Also rated for electrical hazard protection. Imported.
Manufacturer model # RB6766.
- Durable, supportive, and waterproof sport boot with composite safety toe protection
- Tough, water resistant, crazy horse leather upper
- Smooth nylon mesh lining with waterproof membrane for breathable, waterproof protection
- Removable EVA cushion insole insert with sponge rubber heel wedge
- PU cushion midsole for cushioning and support
- Aircom™ heel airbag - greater impact resistance and walking comfort
- PU Direct Attach Construction - tough watertight bond, less weight, greater flexibility
- Dual density, durable traction rubber outsole with work-friendly 90º heel
- Oil-, abrasion-, and chemical-resistant rubber outsole with slip resistant tread design
- Lightweight composite toe protection - meets ASTM 2413 standards
- Airline security friendly design - 100% non-metallic
- Rated for electrical hazard protection
Need Help? 7 days a week: 7am to 10pm CT or call 1.800.227.7776 anytime


text:
Updated 10/15/2013
Gracious, gregarious, and extremely confident, Aromi is easily among the top-tier of Italian restaurants in the city and proud of it. Classic pastas made in-house and fresh seafood shown off tableside are two of the crowd favorites, as are the superb salads and well-chosen Italian wines. With exposed brick, white-washed walls, and wine bottles on display, the dining room imparts a continental feel without being kitschy. Leave room for a post-dessert treat: Aromi stocks an exclusive list of rare grappas. The restaurant offers seasonal, multicourse tasting menus and excellent-value business lunches on workdays.
Mánesova 78, Prague, Bohemia, 120 00, Czech Republic
Website: www.aromi.cz
Restaurant Details:
Updated 10/15/2013
No Thanks


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- Filed Under
Hundreds of people will help raise money for cancer research during the annual Relay for Life event outside Two Rivers High School on Friday and Saturday. The Relay, however, is much more than a traditional fundraiser.
Beginning with the high-energy opening ceremony at 6 p.m. Friday and concluding with the closing ceremony at 9 a.m. Saturday, the overnight "campout" brings together those who have fought directly on the cancer battlefield. Some have won the battle; others have succumbed, but all share that special bond of having been in the fray against the insidious disease.
The event is mainly about hope and progress. Teams and individuals run laps continuously around the high school track - relay style - as a reminder of the ongoing fight against cancer. They have solicited more than $77,000 in donations in advance of their efforts, and people are welcome to stop by and donate at the site. The Manitowoc County Relay for Life also accepts donations via its website, through the American Cancer Society.
Following the opening ceremony, participants who have survived cancer take a lap around the track, followed by a lap devoted to those who provide care to cancer patients. At 9 p.m. as it gets dark, the moving Luminaria Ceremony is held to remember those who have died from cancer, and to also honor those who have fought cancer in the past.
Relay for Life has become a phenomenon throughout the United States and internationally. It began in the mid-1980s with a single individual, Dr. Gordy Klatt, a Tacoma, Wash., colorectal surgeon, raising money by circling the track at Baker Stadium at the University of Puget Sound in Tacoma. A marathon runner, Klatt ran for more than 83 miles as friends paid $25 to run or walk with him for 30 minutes.
In 1986, 19 teams took part in the first Relay event at the stadium and raised $33,000.
Each Relay event is unique, but all have in common a survivor's lap, luminaria lighting and the Fight Back Ceremony, where people make a personal commitment to save lives by taking up the fight against cancer. According to AmericanCancerSociety.org, that may include such things as getting a screening test, quitting smoking, or talking to elected officials about cancer.
"By taking action, people are personally taking steps to save lives and fight back against a disease that takes too much," according to the website.
We can all be grateful to organizers of this event in Manitowoc County for bringing help - and hope - to those afflicted with cancer.


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Students who sign up for a personal computing class through the St. Charles Community College Continuing Education Department this fall will be eligible for free refresher courses within the next year. The refresher course must be identical to the original class.
"We want to give our students a chance to take refresher courses for personal computing classes they've already taken," Kathy Stearns, continuing education program specialist. "We understand how hard it is to remember everything from those classes."
The SCC Continuing Education Department offers personal computing classes for all ages that teach the basics and beyond. The classes offered include training on QuickBooks, Adobe programs (Dreamweaver, Fireworks, Illustrator, InDesign and Photoshop), Microsoft applications (Excel, PowerPoint, Word, Outlook and Publisher), as well as more basic classes, such as "Beginner's Guide to Computers," "Computer Tutoring" and "Going Google."
Class start dates, times, number of sessions and costs vary. For more information on personal computing classes, class schedules and costs, or to register, visit stchas.edu/learnforlife or call 636-922-8233.
St. Charles Community College is a public, comprehensive two-year community college with associate degrees and certificate programs in the arts, business, sciences and career-technical fields. SCC provides workforce training and community-based personal and professional development as well as cultural, recreational and entertainment opportunities. For more information, visit http://www.stchas.edu.
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International Student Services
Welcome to the International Students Services at North Carolina Wesleyan College! Our objective is to welcome and serve all our international students by answering their questions on a variety of topics and by helping them to adjust to a new culture as well as a new educational system. International Student Services also offers English language support for all our international students through individual and group tutoring and consultation. For more information please contact Gene Heavner at 252.985-5124 or email@example.com