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NF-κB is a master gene regulator involved in plethora of biological processes, including lymphocyte activation and proliferation. Reversible ubiquitinylation of key adaptors is required to convey the optimal activation of NF-κB. However the deubiquitinylases (DUBs), which catalyze the removal of these post-translational modifications and participate to reset the system to basal level following T-Cell receptor (TCR) engagement continue to be elucidated.
Here, we performed an unbiased siRNA library screen targeting the DUBs encoded by the human genome to uncover new regulators of TCR-mediated NF-κB activation. We present evidence that knockdown of Ubiquitin-Specific Protease 34 (USP34) selectively enhanced NF-κB activation driven by TCR engagement, similarly to siRNA against the well-characterized DUB cylindromatosis (CYLD). From a molecular standpoint, USP34 silencing spared upstream signaling but led to a more pronounced degradation of the NF-κB inhibitor IκBα, and culminated with an increased DNA binding activity of the transcription factor.
Collectively, our data unveils USP34 as a new player involved in the fine-tuning of NF-κB upon TCR stimulation.
Keywords:DUBs; NF-κB; Ubiquitinylation; T-Cell receptor
Nuclear factor-κB (NF-κB) transcription factors initiate transcription of genes essential for mounting an adequate immune response [1]. Ubiquitously expressed NF-κB heterodimers of Rel family proteins are normally sequestered in the cytosol of the cells by Inhibitors of NF-κB (IκBs) proteins [2]. In lymphocytes, the ligation of antigen receptors assembles the so-called CBM complex that consists of the scaffold CARMA1 and the heterodimer BCL10/MALT1 [3]. The CBM microenvironment drives oligomerized BCL10 and MALT1 to undergo K63-linked non-degradative ubiquitinylation [4-7]. This authorizes the recruitment and activation of the IκB kinase (IKK) complex that comprises two catalytic subunits (IKKα and IKKβ) and a regulatory subunit (NEMO, also called IKKγ) [8]. IKK phosphorylation of IκBs precipitates their K48-linked ubiquitinylation and proteasomal elimination, and thereby allows NF-κB to translocate to the nucleus where it binds DNA and initiates transcription [8]. NF-κB-dependent neosynthesis of IκBs subsequently drives NF-κB to shuttle back to the cytosol [1]. Although reversible ubiquitinylation processes are central for T-cell receptor-(TCR)-mediated NF-κB activation, the deubiquitinylases (DUBs) in charge of trimming these poly-ubiquitin chains to ensure optimal signaling, as well as to reset the system to basal levels remain poorly defined [9]. Thus far, two DUBs, namely cylindromatosis (CYLD) and A20 (also known as TNFAIP3), were demonstrated to negatively regulate antigen receptor signaling [9,10]. Herein, we provide evidence that Ubiquitin-Specific Protease 34 (USP34) also contributes to the fine-tuning of NF-κB upon TCR engagement.
To identify additional negative regulators of TCR-mediated NF-κB activation, we conducted a siRNA library screen against 98 DUBs through a gene reporter luciferase assay in Jurkat T cells stimulated with either anti-CD3 and anti-CD28 antibodies or PMA plus ionomycin to mimic TCR engagement (Figure 1A and Additional files 1 and 2). As expected, CYLD silencing led to an enhanced NF-κB activity upon TCR stimulation (Figure 1A). Furthermore, this screening also uncovered siRNA sequences specific for USP34 that potentiated NF-κB activation with a similar magnitude to CYLD siRNA (Figure 1A). USP34 encompasses a 404 kDa protein with a central catalytic domain [11]. However, little is known about this DUB, albeit it was previously linked to the Wnt developmental signaling pathway [12]. Subcellular fractionation experiments showed that USP34 was essentially distributed in the cytosol of cells regardless of TCR stimulation, and was notably absent from the nucleus and organelles (Figure 1B and Additional file 3A). We next verified by immunoblot that CYLD and USP34 endogenous levels were efficiently decreased by their respective siRNA sequences (Figure 1C). Of note, an additional siRNA duplex specific for USP34 was also included to reinforce our initial findings (named sequence 3). Consistent with the primary screening, NF-κB reporter activity was similarly boosted upon TCR stimulation in USP34- and CYLD-silenced Jurkat when compared to control non-targeting siRNA transfected cells (Figure 1D and E). As a consequence, the levels of the NF-κB targets NFKBIA (IκBα), interleukin-2 (IL-2) and TNFα, as measured by RT-PCR were increased in USP34-knocked down cells (Figure 1F). Accordingly, downstream IL-2 secretion was enhanced in supernatants of USP34-silenced cells (Figure 1G). Finally, ectopic expression of a plasmid encoding for the catalytic domain of USP34 (USP34-CD [13]) markedly dampened TCR-mediated NF-κB activity (Figure 1H). Because USP34-CD is a large segment (383 amino acids), it is possible that in addition to the catalytic domain, it also comprises a domain required for the binding to its partners to regulate NF-κB in lymphocytes. Collectively, our data suggest that USP34 is a cytosolic protein, which functions as a negative regulator of NF-κB upon TCR engagement.
Figure 1. Identification of USP34 as a negative regulator of TCR-mediated NF-κB activation. (A) NF-κB reporter luciferase assay screen of a siRNA library targeting 98 DUBs (2 siRNA/target) in Jurkat T lymphocytes stimulated with 20 ng.ml-1 PMA plus 300 ng.ml-1 ionomycin (P/I, top panel), or with 0.5 μg.ml-1 anti-CD3 and anti-CD28 (bottom panel). Fold activation compared to non-targeting (n.t.) siRNA-treated cells is shown. Green and red histograms indicate siRNA against USP34 and CYLD, respectively. (B) Nuclear (Nucl.) and cytosolic (Cyt.) fractions from Jurkat T cells stimulated with P/I as in (A) for 0 and 15 min were analyzed by immunoblot. PARP and tubulin served as loading and purity controls for nucleus and cytosol, respectively. Molecular weight markers (M.W.) are indicated. (C) Lysates from Jurkat cells transfected for four days with siRNA against CYLD, USP34 (three individual sequences), or with control n.t. siRNA were analyzed by immunoblot as indicated. (D and E) NF-κB reporter luciferase assay (mean ± S.D. of triplicate experiments) of n.t.-, CYLD- or USP34-silenced Jurkat cells stimulated as in (A). RLU, Relative Light Units; Unst, unstimulated cells. (F) Cells as in (C) were stimulated with 20 ng.ml-1 PMA plus 300 ng.ml-1 ionomycin (P/I) for 1 and 2 hours. mRNA levels of NFKBIA (IκBα), IL-2, TNFα, and ACTIN were measured by RT-PCR. (G) Enzyme-Linked ImmunoSorbent Assay (ELISA) of IL-2 secreted in the supernatant of Jurkat treated as in (C) and stimulated with P/I. (H) NF-κB reporter luciferase assay of Jurkat cells transfected with the catalytic domain of USP34 (V5-tagged USP34-CD) or with a control empty vector (EV) and stimulated as indicated. Histograms represent mean ± S.D. of triplicate experiments. Inset blot shows USP34-CD expression when overexpressed in HEK293T cells.
Additional file 1. Methods description.
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Additional file 2. Design of the siRNA library screen.
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Additional file 3. BCL10 and MALT1 ubiquitinylation, CBM assembly, and IKK phosphorylation in USP34-silenced cells.
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In addition to NF-κB, TCR ligation kindles various signaling pathways including Nuclear factor of activated T-cells (NFAT) and the Mitogen-activated protein kinase (MAPK) Extracellular signal-regulated kinases (ERK) [14]. Gene reporter assays showed only modest increase in NFAT activation in USP34-silenced when compared to control cells (Figure 2A). Furthermore, ERK phosphorylation occurred normally without USP34 (Figure 2B). Keeping with this, no overt change in the general pattern of tyrosine phosphorylation was observed upon TCR stimulation, further arguing against a general impairment of TCR signaling in the absence of USP34 (Figure 2C). We next investigated whether USP34 also curtailed NF-κB activity emanating from TCR-autonomous signaling triggers. To this end, USP34-silenced Jurkat cells were stimulated with the cytokine TNFα or with the genotoxic stress agent etoposide that functions via an unconventional ATM/PIASy/Sumoylated-NEMO axis [15]. Paralleling the situation with TCR, knocking down USP34 markedly increased NF-κB in cells treated with TNFα or etoposide (Figure 2D). Supporting previous studies with CYLD-deficient cells [10,16], CYLD silencing in Jurkat cells also increased TNFα- and etoposide-mediated NF-κB activation (Additional file 4). Combined, these results indicate that USP34 shares some functional similarities with CYLD and selectively targets the NF-κB signaling pathway.
Figure 2. Knockdown of USP34 selectively potentiates NF-κB activation. (A) NFAT reporter luciferase assay (mean ± S.D. of triplicate experiments) of Jurkat cells transfected with control non-targeting (n.t.) or USP34 siRNA and stimulated with 0, 0.1, 0.5, and 1 μg.ml-1 anti-CD3 and anti-CD28 antibodies (αCD3/28). RLU, Relative Light Units. (B) n.t.- and USP34-silenced Jurkat cells were stimulated with 20 ng.ml-1 PMA plus 300 ng.ml-1 ionomycin (P/I) for 0, 7.5, 15, 30 and 60 min. Cell extracts were prepared and analyzed by immunoblot as indicated. Molecular weight markers (M.W.) are shown. (C) Cells as in (B) were stimulated with 1 μg.ml-1 anti-CD3 and anti-CD28 antibodies. Cell extracts were prepared and general tyrosine phosphorylation pattern (P-Tyr) was evaluated by immunoblot. GAPDH served as a loading control. (D) NF-κB reporter luciferase assay of Jurkat cells transfected as in (A) and stimulated with 10 ng.ml-1 PMA plus 300 ng.ml-1 ionomycin (P/I), with 10 ng.ml-1 TNFα, or with 40 μM etoposide (VP16). Shown is the mean ± S.D. of triplicate experiments. Unst, unstimulated.
Additional file 4. CYLD and USP34 silencing enhanced etoposide- and TNFα-mediated NF-κB activation.
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To gain insights on the signaling basis for the exacerbated NF-κB activity in USP34-depleted cells, we first examined BCL10 and MALT1 ubiquitinylation status since it governs the strength of TCR-mediated NF-κB activation [4-6]. BCL10 ubiquitinylation, which can be assessed in fractions enriched with heavy membranes [17], remained unchanged without USP34 (Additional file 3A). Moreover, pull-down of CK1α to precipitate the CBM complex [7,17], showed similar amounts of ubiquitinylated MALT1 bound in both control- and USP34-siRNA transfected cells (Additional file 3B). Keeping with this, BCL10 association to CARMA1 occurred normally without USP34 (Additional file 3C). We finally evaluated the impact of USP34 on the phosphorylation of IKK, which reflects its activation [8]. IKK phosphorylation was not exacerbated in those cells, and rather appeared slightly decreased (Figure 3A, and Additional file 3D). Although puzzling, this might result from a feedback loop triggered by enhanced NF-κB. We next assessed directly NF-κB DNA binding activity. Consistent with the gene reporter assays, more active NF-κB-DNA complexes were detected in nuclear extracts from TCR stimulated cells when USP34 was silenced (Figure 3B). Although no obvious difference in NF-κB subunit p65 translocation into the nucleus was detected, the degradation of the primary NF-κB inhibitor IκBα was more pronounced in cytosolic fractions from USP34-silenced cells when compared to control cells (Figure 3C). Accordingly, IκBα degradation was also prolonged or more dramatic in lysates from cells transfected with USP34 siRNA even on longer time points up to 3 hours (Figure 3D). Hence, our results suggest that USP34 likely functions downstream of the CBM-IKK nexus to enhance NF-κB activation.
Figure 3. Silencing of USP34 enhances IκBα degradation and NF-κB binding to DNA. Jurkat cells were transfected with non-targeting- (n.t.) or USP34-targeting siRNA for 72-96 h. (A) Lysates from cells stimulated with 40 ng.ml-1 PMA plus 300 ng.ml-1 ionomycin (P/I) for 0, 10, 30 and 60 min were analyzed by immunoblot as indicated. M.W., molecular weight markers. (B) NF-κB binding to DNA was assessed on nuclear extracts from cells stimulated as in (A) for the indicated time by non-radioactive electrophoretic mobility shift assays (EMSA) using biotin-labeled κB element DNA sequence. Note that DNA:p65 complexes were chased away with a cold probe, and were shifted when a p65 antibody was added. (C) Nuclear and cytoplasmic extracts from cells stimulated as in (A) were analyzed by immunoblot as indicated. GAPDH and PARP served as loading controls. (D) Lysates from cells stimulated as in (A) were assessed by immunoblot as indicated.
Almost 100 DUBs were identified in the human genome and yet, only a few have been ascribed a function [11]. As for TCR signaling, the well studied A20 and CYLD thwart NF-κB at different levels [10]. CYLD targets and inhibits the ubiquitin-dependent IKKβ kinase TAK1 and therefore prevents aberrant lymphocyte activation [18,19], while A20 dampens NF-κB activity by trimming K63-ubiquitin chains attached to MALT1 [20,21]. Our study now unveils USP34 as an additional negative regulator of NF-κB in lymphocytes. How USP34 tempers NF-κB activity remains unclear. In contrast to CYLD and A20, which target apical signaling [18,20], USP34 rather seems to function downstream of the IKK complex. Given that USP34 does not bind to the NF-κB core components (our unpublished results), we favor a model in which USP34 impacts on the activity of a cytosolic co-activator to ensure IκBα fine-tuning [22,23]. Alternatively, USP34 might also intervene in other checkpoints to control NF-κB signal outcome and intensity such as post-translational modifications, nuclear shift, or DNA three-dimensional structure [22,24,25]. Nevertheless, our data illustrates how various layers of control cooperate to ensure the fine-tuning of NF-κB following engagement of the TCR.
CYLD: Cylindromatosis; DUBs: Deubiquitinylases; ERK: Extracellular signal-regulated kinases; IκBs: Inhibitors of NF-κB; IKK: IκBs kinase; MAPK: Mitogen-activated protein kinase; NFAT: Nuclear factor of activated T-cells; NF-κB: Nuclear factor-κB; PMA: phorbol 12-myristate 13-acetate; TCR: T-Cell receptor.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
KP designed and conducted most experiments, analyzed the data and wrote the manuscript. EMH, NC, SMD, HML, CL, and CA designed and conducted experiments, and analyzed the data. EMH and JG helped drafting the manuscript. JG and AV analyzed the data. NB conceived the project, analyzed the data and wrote the manuscript. All authors read and approved the final version of the manuscript.
We thank V. Quesada and C. Lopez-Otín (Universidad de Oviedo, Spain) for providing USP34-CD plasmid, and D. Arnoult (INSERM U1014) for helpful discussions. This work was supported by grants from ANR JCJC, Fondation ARC, Fondation de France, Ligue Nationale contre le Cancer, and by Fondation pour la Recherche Médicale. KP and SMD are supported by fellowships from Université Paris Sud, and EMH by la Ligue contre le Cancer.
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Comments by Evil Queen
I know tvd isn't know for its great wardrobe but wtf,Bonnie looks like a complete hobo in this pic,i mean even Elena the original hobo of this show looks better in comparison.jeez
Lol,wtf was that!*DEAD*
whoever does this promo shots needs to lay off ps for a while.lol wtf is this and more importantly why the fuck is this??lmao
wow this round table is really biased.Caroline is whiny just because she hates Damon for what he did to her and looking out for her friends?oh okay.
So Conrad's banging Ashley,no surprises there.is there anyone on the show who doesn't lie to Daniel though lol.and i hope the body covered in blood is Jack's.he seriously needs to gtfo of the show.
i meant 5-4 epi's lol
ugh why do they need 546 guest actors,i mean you can't even properly develop the main characters you have and still you have to get a new character for every 54-5 epi.seriously.
ofcourse the hate shifted from Caroline to Stefan lol.Is there anyone you Damon stans don't hate.srsly..Damon is allowed to kill people after getting rejected and Stefan can't even lash out at the reason of his break up.lol hypocrite much.

subset: commoncrawl


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The body politic: theorizing disability and impairment
Cole, Phillip (2007) The body politic: theorizing disability and impairment. Journal of Applied Philosophy, 24 (2). pp. 169-176. ISSN 1468-5930
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Marketplace for Thursday, September 27, 2012
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Defense contractors have warned for months they'll have to lay off thousands of workers if Congress takes us over the fiscal cliff at year's end. As crunch time approaches, how serious are they? Sears and Darden Restaurants have a new plan for employees' health care. The NFL and its referees union have agreed to a new, more lucrative contract. That got us wondering, what does it take to be a ref? Gallup's Frank Newport discusses why there hasn't been a major third-party presidential candidate in this election cycle. Plus, we look at Spain's tough new budget, the bankrupt city of San Bernardino, Calif., and author Nate Silver on statistical modeling.
The problem with statistical models and forecasting
Posted In: statistics, forecasting, numbers
Author Nate Silver discusses why he believes that numbers are part of the challenge in painting a clear picture of the American economy.
Heartbreak Hotel: Lil Wayne passes Elvis for Billboard record
Posted In: Elvis, Lil Wayne, billboard
Rapper Lil Wayne has surpassed Elvis Presley's record of appearances on Billboard's Hot 100 chart.
Spain's government announces tough new budget
Posted In: Europe debt crisis, spain, austerity
In further austerity measures, the government imposes some $50 billion of tax hikes and spending cuts
Missing this election: Third-party presidential candidates
Posted In: 2012 election
1992 was a banner year for the third-party candidates. Like this election cycle, the economy was bad and people were concerned about the national debt. But no third-party candidate like Ross Perot has emerged.
Pick-your-policy health care
Posted In: health insurance
Instead of offering policies to choose from, some big employers will be offering workers a lump sum and letting them shop for their own health care.
Defense layoffs or bluff?
Posted In: fiscal cliff, defense contractors
Contractors say 'fiscal cliff' could force layoffs; legal deadline for pink slips looms
San Bernardino struggles after bankruptcy
Posted In: San Bernardino, municipality
Long struggling, San Bernardino couldn't weather the storm of the 2008 recession. The city of more than 200,000 has declared bankruptcy, and that's just one of the big problems it's facing.
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At Asda, we're committed to working with local suppliers. This helps our local communities while bringing you a fresh and tasty product.
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We think it's important that we help out local businesses, while still being able to bring you the best quality products at everyday low prices.Read more about our local suppliers


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SponsorshipsCorporate sponsorship opportunities are available for many different Zoo programs and offerings from our trackless electric train and ZooMobile, to the Wildlife Stageshow and Zoo Key talk boxes. Let us tailor a sponsorship to meet your needs.
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Onsite SamplingThe Sacramento Zoo is available as a venue for a variety of product sampling. With almost 500,000 visitors a year, most being families with young children, we are a great place to promote your existing products or introduce something new into the Sacramento market.
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Star Trek: Full Speed Ahead #6: "A Great Perhaps"
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Re: Star Trek: Full Speed Ahead #6: "A Great Perhaps"
Act Four
Captain Andrew Estrada of the USS
patted Jesse Kincaid on the back. "Good to see you, again." They stood within the turbolift as it ascended toward deck one. "It's been what... four years since
Kincaid smiled. "A little under four, yes, sir." The lift came to a halt and the doors parted to admit them entry to the main bridge. He led the captain to the other side of the bridge and into the corridor leading to the observation lounge.
The commanding officer of USS
, Captain Nathan Brotman, had already been seated to Leone's left and appeared to be mid-conversation when Kincaid and Estrada entered. Upon seeing Estrada, however, Leone excused herself to walk to Estrada and greet him.
Kincaid said nothing as his captain leaned in to give Estrada a peck on the cheek. In response,
's captain half-smiled.
"Andy, I'm glad you're one of the guys I'm handing over this mission to," Leone said warmly. She turned to Kincaid and explained, "We served together on
under Captain DeSoto for a couple of years before he won that XO spot on
"Small universe," said Andy with a grin. "Jess took over for me as exec on
when I earned my fourth pip."
Leone breathed, "No kidding." She gestured with an open hand to the seat that Kincaid normally occupied. "Andy, if you please. You've met Captain Brotman, I'm sure?"
"Not in person, but we've had a lot of calls," Andy replied. Before sitting down, he offered his hand to Brotman, who accepted it. "Captain, good to finally meet you."
Brotman flashed a smile toward Andy. "Likewise, sir."
"Jesse," Leone said, "why don't you call in Ariel, Abbie, Petra, and Isira?" As Kincaid turned to summon them, Leone turned her attention back to Andy. "So, I heard they've got you roaming the Black Cluster?"
"Yes, but not for much longer," Andy said. "I'm being told that we'll be shifted to the Romulan border in the next six months as the command cruiser out of Deep Space Four."
Abbie and Isira arrived together from the same door that Kincaid used, while Ariel and Petra used the opposite entrance near the turbolifts. Kincaid could not help but notice that both visiting captains immediately fixated on Ariel's presence.
Leone made the quick introductions and then turned over the briefing to Kincaid and Abbie.
"Sirs, as you know, we've collected quite a bit of data, all of which has been transmitted to your ships. We indicated where we left off in our analysis, as well as where our ships left off in the search grids of the debris field. So far, we still haven't found any evidence of anything other than an accident causing the initial explosion. Likewise, we have yet to find any evidence that Ensign Thomas O'Day managed to evacuate the shuttle prior to its destruction," Kincaid summed up succinctly before looking expectantly at Abbie.
"We also forwarded to your Chief Engineers the enhanced sensor package we were using to cut through the distortions left by the freighter's cargo explosion," the lieutenant explained. "Lieutenant Bartlet has outlined the hardware modifications necessary, while I included the program modifications which you would simply be able to upload into your computers. We estimate that you can make the modifications in no more than twenty minutes per shuttle."
Andy asked, "I presume that you were able to make these modifications to some or all of your shuttles?"
Petra replied, "More or less, Captain. To be honest, we did not have enough stores to utilize across all forty-seven- er, forty-six of our shuttles, pardon me. We were only able to modify eleven shuttles before we began our recall."
"And this was a project undertaken by your division, Mister Bartlet?"
"In conjunction with Lieutenant Atherton, yes, sir," Petra turned her head to acknowledge Abbie.
Andy grinned. "I had a sneaking suspicion."
Leone tilted her head slightly. "I don't understand."
"We served together on
, sir, for a little over a year," Abbie explained to Leone.
"Had I an open berth for a chief science officer, I think I might've fought you over her," Andy said with a smirk. "Please continue with the briefing, Commander."
Kincaid waited a beat, taking in Leone's slight nod of approval, before noting, "We also included two other search patterns for you to consider. These patterns were based on the analysis we completed and are designed to focus on areas we think are more likely to contain any trace of the ensign."
Brotman leaned forward. "But, this is for recovery, right? It's been well over forty-eight hours, and those suits aren't really given to extending their ranges no matter what you do to them. Surely, you're not holding on to some hope that he's still alive."
As Brotman was speaking to Kincaid, Leone bristled at the mention of the ensign's death. Isira noticeably picked up on that and cleared her throat to distract Leone before she could say anything.
Kincaid gave the captain a bland look, though there was nothing mild about his tone. "Regardless of his possible survival, sir, I believe it is our duty to try and recover what we can for his family to mourn properly."
Andy nodded. "I agree. Whether we rescue or recover the ensign, we should make every expedient effort to locate him." He set his eyes on Brotman as he spoke, "We do not leave our own behind." As he said it, he realized his mistake and added, "I meant no disrespect, Captain Leone. Your ship was ordered away."
Leone stared at the long table. "Of course not, Andy." Though she said the words, Isira knew there was very little truth behind them. "Besides, we're all one fleet."
=/\= =/\= =/\= =/\= =/\=
Isira asked Leone and Kincaid to remain behind once the briefing concluded and the captains returned to their respective starships.
"What can we do for you, Counselor?" asked Leone.
"Sir, I'd like for you to strongly consider conducting a memorial service for Ensign O'Day." Off their uncomfortable expressions, Isira added, "Preferably while we're en route back to Starbase 310." She put up a hand to forestall any disagreement for the time being. "The crew is very upset. Very few people are ready to deal with this kind of loss when on this kind of mission. I think it is important to let people know that it is okay to begin to grieve even though we don't yet have his remains."
From Kincaid, Isira could sense his reluctant acceptance of her argument. "Captain, I have to agree with the Counselor. It would give the crew some closure after the frantic search we just went through."
Leone's uncertainty was replaced with discontent at their words. "A memorial service is also one way of saying to the crew that we've given up on the ensign. You don't think that would have an adverse impact on morale to know that their captain will throw in the towel if they were floating out there waiting for a rescue?" As she spoke, each word had more edge than the previous until its sharpness was unmistakable.
"No one thinks so, sir. I can assure you of that," Isira answered forcefully. "Moreover, the ceremony I had in mind was truly in keeping with the sentiments of a memorial. We will remember him, keep our thoughts with him though we cannot keep searching. We will give the crew a safe space to talk about Ensign O'Day without the conversation crashing to an uncomfortable halt."
The captain's jaw visibly flexed as she considered her response, though Isira could already tell that Leone's emotional state was in severe flux. Finally, after a few minutes of contemplation, she conceded. "Fine. Make the arrangements. Is there anything else?"
"Sir, would you like to speak with me privately?" Isira questioned. "If not now, then a bit later would be fine."
Before Leone could answer, Kincaid took that moment to lift himself from his seat. "By your leave, sir?"
Leone's gaze drifted from Kincaid to Isira. "That will be all, Jesse." Once they were left alone within the observation lounge, she continued to stare at the Counselor. "Something on your mind? Or perhaps mine?"
Isira let the joke pass with only a small smile. "I wanted to give you a chance to express what you're going through, sir, before you have to speak about Ensign O'Day publicly. I know this is a new situation for you."
"I've lost subordinates on missions, before."
"Not when you've been the captain of the ship."
Leone sighed. "I've only been captain for all of fifteen minutes, Counselor."
Isira said nothing, trying to prompt the captain to fill the silence herself.
The captain took a deep breath and laid her right palm against the surface of the table to turn away from Isira and look out of the large viewports. "In those fifteen minutes, I've managed to lose one of my crew. You don't think that's a little strange for a new captain?"
"There have been many captains who lose crewmembers the day they gain command, and not just in a time of war. Starfleet is hazardous duty; even routine missions contain dangers. That's why Starfleet is so careful in selecting its captains."
"Yeah, well... I'm not the other captains, Counselor."
"No, you are not. Why don't you tell me what you're thinking, sir?" Isira coaxed.
Leone's flat palm curled up into a clenched fist. "That this wasn't supposed to happen, and certainly not to a kid like Tommy O'Day. He deserved better than this, and he deserves us going to every extent of making sure that he's back on board this ship with the crew where he belongs. And I think that a memorial service is a clear sign that we'd abandoned him, as though leaving him behind literally doesn't already do that!"
"But for the Admiral's orders, we would still be a part of that search effort. Everyone is aware of that. And everyone is always aware of the near impossibility of the ensign's ability to survive in a suit for this length of time. Even though most of your crew won't say it, almost all of them believe he is gone. There are Vulcans, Tellarites and other members of your crew who are less hesitant who voice this sentiment right now. Everyone's just waiting for your permission to mourn."
She drew in a deep breath through her nose and exhaled through her lips. "And I thought I said that was fine. It doesn't mean I have to like it, but I'll handle it like I do everything else."
"If you don't speak honestly about that, sir, they won't see the memorial as anything but a formality."
Leone rose from her seat, clearly finished with the conversation. "Then so be it, Counselor. I need to return to my ready room so I can stop talking to you. You understand, I'm sure." She did not wait for a response, instead leaving Isira alone within the lounge.
=/\= =/\= =/\= =/\= =/\=
Abbie walked down the corridor with Captain Estrada, his informal escort to the transporter room.
"I meant what I said back there, Abbie. If I had the position open, you would have been my first choice," he said with a wide grin. As he did so, the laugh lines around his dark brown eyes formed.
"All from the year I was the most junior lieutenant on the ship, sir?" Abbie questions dryly. "I suppose I should thank you."
"As if I didn't keep tabs on my old ship. And as if Gleason wasn't campaigning on your behalf. I don't think you know half of what the Old Man does for you. If I didn't know better, I'd think you were related," he teased.
"I always thanked him for his recommendations, but really, we didn't serve together for that long. I tell him he doesn't need to chat me up so much."
"Jesus, I've never seen anyone so reluctant to acknowledge that they earned a Silver Star. At twenty, no less, and from someone who didn't go to the Academy. Listen, Abbie, in all seriousness, no one impresses the Old Man. I mean, no one. If he likes you, he calls you competent. The words he uses for you are, and I quote, 'knowledgeable, proactive, and excellent in all categories.' The
Old Man
. The Admiralty probably choked when they read that."
"I just don't like to be known for being lucky enough to survive and quick enough to save my own behind as if that was something anyone else wouldn't do, sir. I want to stand on my own, be known for what I do in my own department," Abbie explained, visibly growing uncomfortable as she guessed at the turn of the conversation.
"And about that. Abbie, you
to consider making a change to Ops down the line. If not Command directly. You have leadership potential all over you and I know that in time, you would make an excellent Captain. But you have to be in the right department for that." Andy grinned. "I see I'm not the first to make that suggestion. Gleason, huh?"
"Yes, sir. And I will tell you what I told him. I am a scientist and I intend to remain one. Respectfully," Abbie added with a smile of her own. "Are you trying to replace my rabbi, sir?" she added, raising a brow.
"I'm not senior enough to shove Gleason aside, but maybe I'm a bit more politic. Either way, we're both doing what we can for you."
"Andy," she replied in exasperation. "Please, don't. It's just so..."
"You don't want acknowledge it, but Starfleet is very political. You don't have the usual network that Academy graduates have, but what you do have is the ability to gain the respect of your commanding officers. Even if we're just helping you to better science positions, let us help you do that. You deserve it and you don't want to be edged out by someone with more strings to pull than you."
"I get it," Abbie accepted resignedly.
"Good," Andy responded triumphantly as they turned to walk through the open door of the transporter room. "So, are you seeing anyone?"
Abbie ignored the sudden smirk of the transporter chief at overhearing the question. "No, sir."
"Because my little brother is still available. I know he didn't graduate from the Vulcan Science Academy but MIT isn't a bad little institution. He'd be a great match for you."
"Thank you, sir, but like I told you before, I'm partial to men in uniform," Abbie answered easily.
Andy laughed as he shook her hand in farewell. "Good answer, Lieutenant. I'm sure you'll speak with Captain Gleason before I do, so please give him my regards. And forward my goodbye to Commander Kincaid as well."
"I will, sir. Good luck on your mission," Abbie replied as he stepped onto the transporter pad.
"Thank you, Lieutenant. See you around," Andy answered before giving the signal to the transporter chief to energize.
Michael D. Garcia
Head Writer, Star Trek: Full Speed Ahead (
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Fri Aug 31, 2012 - 12:22 pm EST
U.S. politics and the Culture of Death
August 31, 2012 (HLIWorldWatch.org) - Election season is upon us, and politics is on everyone's mind. There is always a lot at stake come election time: the direction of the country, the economy, wars and foreign affairs. For Catholics and pro-life activists, this year's presidential election is especially crucial if we are to protect and maintain our constitutionally-guaranteed religious liberties, and win the fight for life around the world.
Politics in the United States has an enormous impact on the international effort to defend life from conception until natural death. For example, there is a direct connection between the person who sits in the White House and how much money the United States spends promoting abortion overseas.
One of the first things President Obama did when he took office in January 2009 was reverse the Bush administration policy that restricted federal funding for international organizations that promote or provide abortions. The Obama administration, in collusion with an anti-life Congress, has since been funneling an average of more than $45 million a year to the abortion-pushing United Nations Population Fund (UNFPA). The Bush administration did not fund UNFPA at all.
If $45 million sounds like a lot, consider the nearly $600 million spent in 2010 alone on the U.S. Agency for International Development (USAID). USAID, under the Obama administration, pushes abortion and contraception around the world. These are just two examples of how your tax dollars are being spent, in the midst of serious domestic economic problems, to promote an anti-life agenda all over the world.
And this money has very real, very devastating effects – especially in the developing world. Human Life International (HLI) Kenya and other Kenyan pro-life organizations spent much of 2010 fighting against the ratification of a new constitution that would open the door for legalized abortion in this traditionally pro-life Eastern African nation. The old Kenyan constitution protected life from the moment of conception. Had the legalization of abortion not been so carefully hidden in its text, it is almost certain that the Kenyan people would have rejected the new constitution. According to a report from the U.S. Inspector General, the Obama administration spent over $23 million to support the new Kenyan constitution – money that went directly to pro-abortion groups engaged in lobbying and get-out-the-vote efforts. Additionally, Vice President Joe Biden and a string of congressmen visited Kenya in the months leading up to the vote, all in an effort to ensure that the referendum was adopted. The U.S. did not just tacitly support the legalization of abortion in Kenya, it aggressively lobbied for it. The United States bought abortion for Kenya with our tax dollars.
Unfortunately, the amount of money being spent to push the deadly sins of abortion and contraception on unsuspecting cultures is not our only concern. The current administration has also launched a direct assault on Catholics and our religious liberties. The now infamous mandate issued by the Department of Health and Human Services is a crystal clear example of the battle we are currently fighting. This mandate will force most Catholic institutions and all Catholic employers to provide health insurance coverage for contraception – which the Church defines as intrinsically evil!
We live in a country that was founded by settlers looking for religious freedom. But the Culture of Death has become so strongly entrenched in our nation that our leaders are poised to force us to violate our religious beliefs and to provide others with something we consider a great moral evil.
It should come as no surprise that anti-life activists have finally publicly set their sights on the Catholic Church. The Church has long been the loudest and often the only voice that cries out in opposition to the evils of the Culture of Death. Can you think of another institution, religious or otherwise, that has remained steadfast in its position on such life-issues as contraception, divorce and marriage? These aren't merely political issues to discuss with our neighbors; they are true and absolute evils. And evil's number one target will always be the Catholic Church, because the Church is the primary means of salvation for the world.
This evil has become so pervasive that society now considers contraception and abortion to be "healthcare" even though there are mounds of evidence that they can be very harmful to those who use them. Various forms of abortion and contraception have been linked to breast cancer(1), depression(2), and even death of the mother(3) (of course, in the case of abortion, there is always also the death of a child).
Even if our liberties were not under attack, we would have a moral obligation as Catholics to vote in November. We must always vote against the evils of the Culture of Death, and this year we must stand up for our own rights as well. Come Election Day, we are the voice of the Church.
Elisa Sumanski works at Human Life International (HLI), the world's largest international pro-life and pro-family organization. A version of this article appeared in HLI's official magazine FrontLines.
1. According to the National Cancer Institute, taking the pill seems to increase one's risk of breast cancer, cervical cancer, and ovarian cancer.
2. The Alfred Psychiatry Research Centre studied 60 women, and found that those taking the pill containing both estrogen and progesterone were almost twice as likely to be depressed as those who did not.
3. Complications from abortion and abortion-inducing drugs like RU486 have caused thousands of deaths. Conversely, in Chile maternal deaths dropped 70% after abortion was banned.
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K&B Transportation Company Profile at OTR Pro Trucker
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text:
new buddy
Look who arrived at my door today? A-D-O-R-A-B-L-E!
From the talented hands of Futuregirl. How do you do it?
I'm in love. No name yet but the first thought that came to me was that he is a super squirrel. The tail kills me.
Look for your mailman, he should have something special for you...
9 comments:
Awesome! He should have won a softie award, don't you think?ReplyDelete
I'm so glad he made it to his new home safely. :) He *is* a cutie, if I say so myself.ReplyDelete
If your little super squirrel ever gets photographed while adventuring, I could add it to my Travelogue section. (totally not expected nor required)
I can't wait for my package. I figured since we were both so silent that we'd finally met our deadline. :) Yay!
That is so fantastic!!!
What a great match for you!
What is his super power?
*swoooooon* Incredible!ReplyDelete
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text: Anna G. DeLorme "Tootles" (nee Davis) on Feb. 23, 2013. Beloved wife of the late Robert, Sr. Devoted mother of Robert, Jr., Maryann, Michael, Perry and the late Carolann. Grandmother of Crystal, Nicole, Bobby and Michael, Jr. Great grandmother of John-John. Sister of Ronald Davis and Roserita Russell. Relatives and friends are invited to her Viewing, Wednesday 9:30 to 10:30 am St. Richard Church, 18th and Pollock Sts., Phila. Funeral Mass at 10:30 am. Interment is private.


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Contact Us: 859.572.6399 • 859.572.6076 (fax) • email@example.com
NKU Department of Music
Northern Kentucky University's Department of Music offers a full range of performance and education opportunities to music majors. Our graduates pursue successful careers as professional performers, college and university faculty, public school music teachers, private studio instructors and in allied fields within the music industry.
Our students have been accepted for graduate study at such renowned institutions as the Juilliard School, Indiana University–Bloomington, The San Francisco Conservatory of Music, University of Wisconsin–Madison, Iowa State University, the Eastman School of Music, and the Cincinnati College-Conservatory of Music.
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text: Don't ever underestimate the value of good quality water for tea brewing. I used to live in an area with very hard water and was completely used to it, so I never gave it any thought. But at work, I made tea with bottled spring water. After a while, I could really tell the difference. …continue reading


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updated 02:05 pm EST, Wed November 22, 2006
HANNSpree sports LCDs
HANNSpree has introduced a new group of sports-themed LCDs. The 15-inch HANNSgolf features a dimpled white-and-green design, with a tee and two club irons providing support. The HANNSdribbling is a 9.6-inch soccer-themed screen, encased in a dome of real soccerball leather. The HANNSball.champions, meanwhile, is another 15-inch display, but is ready for wall-mounting, uses basketball leather, and has an embossed list of NBA Finals champions. The golf and ball.champions sets support resolutions up to 1024x768, while the dribbling is limited to 800x600. All three TVs are 4:3 displays with response times of 16ms. The golf is priced at �389 ($738), the dribbling at �299 ($568), and the ball.champions at �459 ($871).


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November 25, 2015
Hello again everyone!
Just got back from the softball state tournament and unpacked. What a great experience! A big congratulations goes out to all of the coverage area teams that made it to Owensboro this season. It takes a lot of hard work and determination to get there and it should be celebrated. Also, it was wonderful to get to meet some of you and watch your tournament runs!
I know our region teams weren't able to come away with the title, but there were some great stories along the way...
Magoffin County made the school's first appearance at the softball state tournament...congrats to the Lady Hornets and good luck to all of the graduating seniors! I think you'll realize how special that moment is later in life for sure.
North Laurel made another appearance at state...to the Lady Jags I had the pleasure of meeting before they left...good luck at the next level or next year at North!
Letcher County stuck it out at state and played two tough games. Congrats girls and good luck getting back there next year!
The past few days were so much fun covering the tournament...I hope the friendships and bonds the teams formed will always stay strong.
Good luck to all of our coverage area teams next year on their journeys to Owensboro!
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text:
1. What is the inspiration for your Loop name? I'm an engineer and I run thus running + engineer = running_eng which gets shortened to RE occasionally.2. A/S/L/F (age, sex, location, family)? 51, male, Maryland (just outside of DC), father, sister, 2 of the world's cutest nieces.
3. When is your Loopiversary (date of your first blog on the Loop)? I joined the Loop on 23 Sept 2009. Unfortunately my first blogs have been lost to the gremlins that infest software but the first one would have been from about that time, it was right before my first race.4. How long have you been running? I ran a couple of seasons of track in high school and sporadically through college to stay in shape for ROTC. I ran during the few years I was on active duty but when I left the Army I stopped running until the summer of 2009.5. What is your favorite distance to race? The half marathon seems to be the distance I've raced the most but I've pretty much enjoy running anything from a 5k to the marathon. The half marathon is a big enough of an event to justify traveling to a new city but doesn't require quite the same build up or recovery that a marathon does. 6. Do you belong to a local running club? Nope.7. Have you ever been to a Loopmeet or Loopfest? Quite a few now including Philadelphia, Surf City, Rehoboth and Shamrock. My first was a Loopmeet at the 2010 Country Music half marathon; got to meet Zam and RunningChick there.
Meeting Alice at RnR Vegas in 2010.
8. Have you ever lost a toenail? Just part of one. 9. What is your favorite post-race treat? Waffles.10. What one piece of running gear is your must-have item? Shorts - everything else is optional. 11. What socks do you wear when running? Hopefully clean ones. I've been partial to Balega socks in the past but I've used a bunch of different brands. Recently I've gotten some Feetures, Smartwool, and Wrightsocks that work well.12. How do your knees feel? Perfect. Never had a problem with my knees. Hips, back, and feet are entirely different story though.13. If you could go to any one running destination/event, where.what would you chose? Tough question. I'll be running the New York City Marathon in the fall which has been at the top of my wish list for a long time now (and since I will be running I won't count it for my answer to this). I'd like to qualify and run Boston. The London and Tokoyo marathons are also pretty high my wish list. Rigth now though my number 1 dream race would probably be the JFK 50 Miler; I'm not if or when I'll run this (26.2 is a pretty big challenge, almost twice that just seems insane) but I started to think about it a while ago as being the "what do I want to work toward after I BQ?" and this keeps coming to mind.14. Hot or cold--which is worse to run in? At some point either temperature extreme starts to become a real pain. Probably the cold for me, I really dislike trying to run on ice (and pretty much all other times where the coefficient of friction approaches 0).
15. If you have a running inspiration or mentor, who is it? Bangle. Brad and I are the same age but he's been running without a major break unlike my 20 plus year hiatus. He's shown me what is possible to acheive and his times have been a benchmark for me to strive for. 16. What is your running mantra? (If your answer is "I have no mantra" or something lame like that, you must answer "Da do run, run, run. Da do run, run") Okay Mike here it is: "Da do run, run, run. Da do run, run" -- yep no mantra.17. Are there any other runners in your family? My sister. She's a much more casual runner but has completed a few half marathons and shorter races (Philly 2011 - Loopfest - was her first half marathon).18. Roads or trails? Roads. I did my first trail race this last Sunday and it was a blast but I enjoy the roads.19. What's the funniest thing that has ever happened to you on a run? I refuse to answer based on my rights to not admit to looking really silly. We were in high school, it was the weekly long run and we were trying to entertain ourselves. Plus it's probably more embarrassing then funny.20. What is your most memorable running moment? Finishing my first race or my first half or full marathon or the incrediable PR I set at Shamrock are all right up there. I going to go with the half marathon at Philly Loopfest in 2011, one of my slowest half maratons ever.. The race and Loopfest were incrediably important for me because of the healing that occurred that weekend. I had struggled finishing the Chicago Marathon the month before due to a hip injury that still wasn't healed at Philly but I was still able run decently and finish strong. Also, a few weeks before the race my mother passed away -- being able to be around my fellow Loopsters really helped in bouncing back from that. It was also my sister's first half marathon -- sharing the race with her and seeing her DH and my nieces out on the course and then sightseeing with them afterwards was great. Sometimes a great running running moment isn't about conquering a new distance or a setting a new PR or even in the epic struggle of the race itself but rather in the people who you are surrounded by.
"Sometimes a great running running moment isn't about conquering a new distance or a setting a new PR or even in the epic struggle of the race itself but rather in the people who you are surrounded by" Amen. Without the people, it's just a selfish endeavor.
Life's a little better when we run.
Great list! I used to feel secretly competitive with you, but now I can't keep up any more!
Great list! Oh come on a teaser with the embarrassing one huh?
Number 19: Let's see, HS, trying to entertain yourselves? Sounds like us in HS. We had a run where we found this weird green "fruit" that had fallen from a tree. Started tossing it around among ourselves, one guy went long, got lead "perfectly" requiring him to leap over the realty sign in the yard. 6'1" of him gracefully cleared it. Unfortunately he was 6'2".
Great list!
"It is always easy to find an excuse not to do something."
My Blog
Awesome! Although, I think that spring marathon looks ideal!
~Quem corre por gusto nao cansa~
#20 is just perfect. Great list!
#20 is awesome as is the picture of your nieces! Long May You Run. Enjoy NYC, it is an awesome experience!!
#20 is great. You're the first Loopster I ever met! Thanks for playing, Ken.
Run fun! ~Mike
Great list!!
"Sometimes a great running running moment isn't about conquering a new distance or a setting a new PR or even in the epic struggle of the race itself but rather in the people who you are surrounded by."
JFK 50! That's on my radar, albeit not for another year or two at least. Although I may pace my friend who is slightly more ambitious than I am.
There should be something here about your ponytail.
Attitude is everything
Inquiring minds want to know about #19
I agree they are the cutest nieces (but I am the casual running sister so I may be biased). Believe me I am racking my brain to remember the story around #19 but I don't think he shared it at the time.
Whoa, thanks! I love being able to inspire with this blog. And you have about caught me! Now you are inspiring me to get my butt in gear. I'd say let's race NYC, but I am trying to commit to not pushing the pace there.
What a great list, especially #20. Let me know if you get closer on JFK 50. It's in the back of my head too.
Good answers!


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10 Selected SongsItem #: HL.50486751
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Composed by Samuel Barber.
High Voice, Book/CD Pack. Vocal. With BOOK W/CD. 9x12 inches. 32 pages. Published by G. Schirmer, Inc.. (HL.50486751)
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Found in: Modern Music Vocal Sing-Along, Modern Vocal Sheet Music
with a CD of performances and accompaniments including the first publication of two early songs Includes the first ever publication and recording of two early songs: Mother, I cannot mind my wheel, and Hey nonny no! These songs were chosen as being especially useful for student singers. Also includes the little known Slumber Song of the Madonna and Strings in the earth and the air, both published in Barber: Ten Early Songs (50482014). The lovely recorded performances will help teachers and students to become familiar with these songs. Other songs: Bessie Bobtail · The Crucifixion · The Daisies · The Secrets of the Old · Sure on this shining night · With rue my heart is laden.
Songs Include:


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Each September, Feminist Health Center hosts the Robert Eads Health Project in partnership with the Southern Comfort Conference.
This clinic was formed in memory of Robert Eads, a trans man who suffered under the transphobic and discriminatory health care system in Georgia. The Robert Eads clinic provides free services to trans men attending the conference that lack access to health care in their home towns. This project helped pave the way for our Trans Health Initiative program where our mission is to empower people through service, education, and advocacy and to provide trans-sensitive health care on an ongoing basis.


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Ed Fitzgerald, right, speaks to Rep Nina Turner at a meeting of Warren County Democrates, Friday, April 19,2013. (Tony Tribble for the Enquirer)
- Filed Under
Ohio Secretary of State candidate Nina Turner, a Democratic state senator from Cleveland, wants to reverse what she calls an "environment of fear" in Ohio voting.
But the current Republican Secretary of State, Jon Husted, said he's just enforcing voter laws - and if Turner isn't willing to do that, she's not qualified for the office.
Turner this week announced she'll challenge Husted next year for his position as elections chief in one of the nation's top battleground states. ...


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SideKick is not new to us, but T-Mobile has series of cellphones in similar technology which are expected to be launched every year with year number behind. T-Mobile has just announced a new and improved version of its very popular Sidekick model and named it T-Mobile SideKick 2008. And as usual this handset is also mainly comes with powerful features that lets you easy and advanced messaging services.
Other features includes: its smallers than earlier model just 4.7 x 2.3 x 0.7-inches, WQVGA 400 x 240 resolutions, QWERTY keyboard, LCD backlight. Other features of latest version is almost similar to earlier version.
Also T-Mobile has added a online service that lets you customize the design of your SideKick which is one of the latest revolution from T-Mobile it seems. This service is available at price of USD 15 only.
Below is the video of Unboxing Video of T-Mobile SideKick 2008
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The current government shutdown clearly illustrates a dysfunctional Congress and a very punitive executive. Our divided Congress has been unable to pass necessary legislation or even a budget for the last four years. Our Congress seems to focus entirely on winning partisan debate points rather than doing the business of the American people. Meanwhile, the president engages in vindictive, childish and punitive mischief against the general public and our military.
The Democrat Party continues to shout that the voters spoke and "we won the election." The reality is that voters chose a balanced government, preventing a one party rule. They voted for Democrats to maintain control of the Senate and for Republicans to retain control of the House of Representatives. Democrats and the president state they will not compromise, insisting the Republicans have no legitimate right to oppose their policies and must capitulate to their demands. The truth is they represent all of the people and must work together.
Our form of government was designed to prevent tyranny of the masses and one party rule. It deliberately created a government of three equal branches and specified the general duties of each. The House of Representatives initiates all spending legislation and therefore controls the federal government's purse strings. In this spending dispute, the House is doing what the Constitution requires. The Senate confirms, amends, or defeats budget bills passed by the House. The executive branch enforces (not creates) law.
The House of Representatives has sent numerous appropriations bills to the Senate to fund the government during this shutdown. The Senate has refused the traditional up or down vote. Additionally, the president threatened to veto them. It's ironic that the Democrat controlled Senate is now demanding that the House bring to the floor a clean Continuing Resolution (CR) for an up or down vote -- a same action they have been refusing in the Senate for constitutionally required budgets during the last four Congresses.
The president has permitted the IRS, EPA and other government agencies to use their regulatory authority to attack the activities of the Tea Parties, conservative businesses, and those who oppose him or criticize his political agenda. In the shutdown, he's withheld executive action to unnecessarily hurt aggrieved military families and veterans desiring to visit monuments. He's sanctioned IRS audits to intimidate individuals like Dr. Ben Carson who criticize authoritatively the harmful effects of Obamacare and questions the president's ability to lead the country.
Only about 17 percent of the federal government is affected by this shutdown. Most Americans wouldn't notice. So, to amplify the harm, this vindictive president has imposed targeted government shutdowns on activities and functions that maximize the "pain" on the public. His closure of the World War II Memorial while opening government property for an immigration rally was pure hypocrisy. It clearly demonstrates his disregard and disrespect of patriotic Americans. How else can anyone explain why he goes out of his way to use discretionary funds to hire additional staff, police, and rented barrier fencing to close otherwise unmanned and open monuments?
Our commander-in-chief should be doing everything in his vast power to protect those who sacrifice for our country. He is quite willing to send forces around the globe to impose his ill-conceived foreign policies knowing many will sustain tremendous physical and mental injuries. Yet he's failed to provide them with the medical care and help they require and earned. While hiring extra Park Police, his administration furloughed, perhaps illegally, staffs at VA hospitals.
His vindictiveness toward the military has included refusal to pay death benefits to the families of military personnel killed in action. Only public outrage reversed this treasonous conduct. His Department of Defense threatened to arrest contract Catholic chaplains if they voluntarily held services on military bases. Last year's sequestration was designed to disproportionately impose punitive and harmful policies on our military rather than applying these cuts equally to all departments.
So, as we stumble through the government shutdown and into the clutches of Obamacare, which uses problematic websites that lack even common sense controls to prevent more fraud, our government chooses to punish our military and the general population rather than compromise. We can no longer accept a dysfunctional government that is unwilling to compromise and find solutions to the problems that confront this nation. Meanwhile, only the most unobservant, the most partisan or delusional among us want to give the president, a vindictive mean-spirited bully, and our dysfunctional Congress more authority over our lives. Really?


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November 29, 2015
The Robertson County Sheriff's Office has released the name of a woman stabbed to death on Saturday night.
Angel Williams of Brazos County was killed when she was stabbed several times. The 43-year old woman was attending the Glover's Rodeo that was being held on the Robertson County Fairgrounds near Hearne off FM 485. County sheriff officials say Williams was involved in an altercation with another person on the fairgrounds when the stabbing occurred.
It's still unclear what started the fight. Reports say during the fight, a knife was pulled. Both the victim and the individual she was fighting with suffered cuts to their bodies, with Williams' injuries being fatal.
It took several days for the victim's name to be released. The Robertson County Sheriff's office had to locate and notify her family. Officials also had to wait for a formal cause of death. Angel Williams' body was taken to Dallas for an autopsy.
On Thursday, Robertson County Sheriff Gerald Yezak said there had been no arrests made in connection to Williams' death. Yezak said a grand jury was being convened to determined if there was enough evidence to file charges.
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The weather is heating up, and so is the battle against childhood obesity. To inspire tasty alternatives to sugary sodas, a "Rethink Your Drink" flavored water contest will take place this Wednesday afternoon at the Santa Rosa Wednesday Night Market.
Infused waters can be made with fresh fruits, vegetables, herbs and spices, in cool combos such as s cucumbers and mint, watermelon and lime, basil and ginger, nasturgiums and lemon zest.
The contest, sponsored by a North Coast Nutrition and Fitness Collaborative, will feature recipes from nine farmers' markets managers representing 11 markets throughout Sonoma County, including Bodega Bay, Cloverdale, Petaluma, Windsor, Healdsburg, Sonoma and Oakmont, Cotati, Sebastopol and Santa Rosa. .
Apprentices from the Worth Our Weight program will create the refreshing entries. The public is invited to sample the waters and vote for their favorite. The top 3 winners will be announced on the main stage at 6:30 p.m.
This recipe for a refreshing agua fresca recipe comes from Santa Rosa chef, author and teacher John Ash:
Agua Fresca
Makes 3 cups
4 cups coarsely chopped seedless watermelon flesh
1 medium fresh lime, juiced
1 cup crushed ice or 1 ¼ cups ice cubes
1 tablespoon superfine sugar (or to taste)
Orange slices and mint sprigs for garnish
Puree the melon in a blender. Add the lime juice, ice and sugar and blend until smooth. Pour into tall glasses and garnish with the orange slices and mint.


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Best Buy Painting is a family owned, full service painting company, dedicated to providing superior interior, exterior, and specialty painting services to both residential and commercial customers in the Cleveland and Northeast Ohio area. As Cleveland house painters, Best Buy Painting is proud to deliver on job quality, customer satisfaction, and of course, our reputation. Over 80% of our new business comes from previously satisfied customers. Read more »
Best Buy Painting's mission is to approach each project as an agreement, not between a business and its customers, but between partners that wish to create a mutually beneficial, long-lasting relationship.


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Exclusive finds with a new theme each month.
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Gunmetal hardware punctuates the clean white wash of stretch-denim skinny jeans designed with leg-lengthening zippered hems.


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What Makes
Colorado Colorado?
Colorado state officials have unveiled a logo and an accompanying slogan ‒ "It's our nature" ‒ as the focal points of a new Colorado brand. In what is described as the most ambitious branding effort ever undertaken by a state, Colorado is incorporating the logo into every activity of state government from print to signs to collateral to websites. "We have a state flag and seal, but until now Colorado has never had a unified brand," says Aaron Kennedy, the state's Chief Marketing Officer. The symbol was designed by Evan Hecox in response to Governor John Hickenlooper's query: "What makes Colorado Colorado?" LEARN MORE >


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By Brian Feinzimer
MLG Anaheim: This eSporting Life
By Charles Lam
Things Get Medieval in Passion Play
By Joel Beers
By LP Hastings
Roll Over, Beethoven...and Cats?
By Dave Barton
Happy 40th Birthday, Buffalo Exchange!
By LP Hastings
Next to Normal: Rock of the Age
By Joel Beers
"Valentine's Day is an occasion that people use to celebrate love and romance and all the wonderful aspects of relationships," Obel says. "And yet with so many women and girls surviving violence, it's hard to imagine that Valentine's Day would be a day of celebration for them. So in a sense, V-Day is about making Valentine's Day—and every day—a day when women can celebrate their sexuality, their safety and their strength."
"It's just reality, and it celebrates femininity, as well as educating people about the violence that women go through every day," Cal State Fullerton's Erwin said. "I've heard some men are scared by the title alone. They think it's going to be male-bashing right off the bat. But it's the exact opposite. It's a celebration as well as an educational tool that these things happen every day to the women you love."
And then Erwin offers what may be the most quotable quote of this still New Year: "Even if you don't have a vagina, I'm sure you love someone who does."V-Day College Campaign 2002 presents The Vagina Monologues at Cal State Fullerton Titan Student Union, 800 N. State College Blvd., Fullerton, (949) 574-1440. Fri.-Sun., 7 p.m. $14 in advance; $20 at the door; and at Cal State Long Beach University Theatre, 1250 Bellflower Blvd., Long Beach, (562) 972-3593; www.loudrmouth.com. Thurs., Feb. 14, 1 p.m.; Feb. 15-16, 8 p.m. $15 suggested donation at the door.
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Organizing parties for kids is much more complicated than organizing them for adults as in children's party one has to ensure maximum fun & entertainment. If you are a parent you then you must be familiar with the requirement of a fun filled birthday party. Right from the birthday invitations for kids to the food & refreshments as well as the entire evening, everything needs to be loaded with fun. If you wish to give your child a party that he/she and their friends can remember for a long time then it is good to hire the services of professional children's entertainer.
There are several companies in UK that provide children's entertainment services. These birthday party entertainers take charge of your kid's birthday party which starts with providing children's birthday invitations. Birthday invitations for kids are designed in the most attractive manner so that they please the children and make them want to come to the party. A lot of kids games and kids disco party form part of the children's entertainment service provided by children's entertainers London. The children's entertainers also cater to children's days out where kids are taken out for a picnic and are entertained with the most interesting children's games.
Some people also like to hire children's balloon entertainer and children's magician London. Having magic shows in kid's parties can be the most enthralling for the kids as the little ones love magic and are sure to get captivated by it. However, some of the entertainers also provide services for other occasions like family fun days, family gatherings, fun day events and corporate events UK. Crew of the entertainment companies, usually dressed up in special uniforms and attractive dresses ensures that everybody has a great time at the party. Some of the companies also organize magic shows London.
Apart from the kid's parties and family gatherings, corporate houses also avail the services of such entertainment companies for corporate fun days and special events. Employers organize corporate fun days so that their employees can relax and enjoy with their families so as to increase the sense of belongingness among them. London corporate events are especially quite popular for the entertainment and fun provided in them by these professional entertainers.
Children's entertainers are quite popular in different parts of UK including children's entertainers Essex, children's entertainers Kent, and children's party entertainers London. Most of these companies have their online presence. So one can easily check their websites and know about the services provided by them as well as their event schedule with the rates. Hence, it becomes quite easy to select the children's entertainer who can fulfill your expectation within your budget.


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How Much Do Online Colleges Typically Cost?
There are various costs involved with attending an online college, and many things to consider when estimating the overall cost of your education. Keep reading for more information.
Overview of Online College Costs
The cost online colleges will vary depending on the school and program. According to U.S. News & World Report (USNWR), online program costs can be determined by a number of factors. For example, some online programs award credit for life experience or previous college coursework, thereby relieving you of certain credit hours you might otherwise have to pay for in a traditional school (www.usnews.com). On the other hand, USNWR reported a survey from Babson Survey Research Group that found that traditional sources of funding for college, like scholarships, are less likely to be available to online students.
The Babson survey also found that tuition and fees for online students are usually the same as for on-campus students. Schools may have different methods of charging tuition which, while not affecting the total costs, can affect the amount of money you pay at any given time. Traditionally, attending an out-of-state college costs more than attending an in-state school. This is not always the case, but it is something to consider when trying to determine the overall cost of going to school.
Additional Costs
When you attend an online college, you are not just charged tuition. Extra costs and fees can add up and increase the overall cost of an online education. In an online program, you need to have access to a computer with an Internet connection. You may also be charged extra for shipping your books to your home and for software programs that you need for certain classes.
Benefits That Offset Costs
Despite the additional costs associated with attending an online college, you may actually save money in the long term due to other aspects of this type of course delivery. Going to college online allows you to have more control over the indirect costs associated with college. For example, online education is administered on a flexible schedule, allowing you to continue working while attending classes instead of having to cut back your work hours or stop working completely. You can also save money by not having to commute back and forth to the campus for classes.
If you're lucky enough to be able to live with your parents, you may be able to save a substantial amount of money by avoiding a dorm or other expensive on-campus housing. According to the College Board in 2013, the average expenses for room and board for full-time, undergraduate students at a 4-year public college for the 2013-2014 school year came to $18,391; non-profit private colleges charged over twice that amount (www.collegeboard.org).