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Shahrukh goes out of his way for friendsSat, Jun 06, 2009 16:45 IST
Despite having vowed to not do anymore cameos in films, Shahrukh Khan went out of his way to make a guest appearance in good friend Makrand Deshpande's film 'Shahrukh Bola Khoobsurat Hai Tu'.
The movie which carries the star's name went on the floors on November 2 last year, which incidentally was also SRK's birthday. But its idea germinated many years ago when Makrand was working with SRK in Swades and proposed to cast the actor in a small role in his directorial venture.
Ever since the shoot began, Makrand has been in touch with Shahrukh, but the superstar was extremely busy and later injured his shoulder. Recently, the King Khan called Makrand at his house Mannat to finalize the shoot.
Makrand needed to shoot a scene in which SRK, playing himself, compliments a flower selling girl on a traffic signal and tells her she's beautiful. The girl is the story's protagonist.
During the shoot held recently, SRK not just allowed Makrand to shoot at his bungalow but also lent his personal car for a scene. Shahrukh completed his scenes before leaving to shoot for Karan Johar 's film My Name Is Khan abroad.
When it comes to cameos, Shahrukh finds hard to say no to friends. Last year, apart from Rab Ne Bana Di Jodi he mostly did cameos in films. But after a bad experience with producer-cum-friend Vivek Vaswani for his film Dulha Mil Gaya , SRK made it clear that he won't do cameos anymore.
However, since he had given his word to Makrand much earlier, the superstar stood by his promise.
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Mac OS X Leopard "Welcome" movie surfaces
updated 10:25 am EDT, Mon August 27, 2007
Leopard "Welcome" movie
A copy of Apple's Mac OS X 10.5 Leopard introduction video has surfaced on YouTube, displaying the company's traditional "Welcome" message in numerous languages set atop a deep-space theme. A simple techno beat accompanies a warm introduction for new Leopard users when installation completes. Mac OS X Leopard is officially scheduled to ship in October, but Apple released developer-only copies at its World Wide Developer Conference in San Francisco as early as June. The Cupertino-based company announced that Leopard would see delays as a result of the iPhone shipment, but released a Leopard early start kit with a beta version in late July.

subset: commoncrawl


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|View single post by Albert Sailhorst
|Posted: Tue Jul 11th, 2006 05:19 pm
Albert Sailhorst
I just finished reading "River Run Red" which is about Ft. Pillow. In the book, there are several quotes from soldiers, officers, etc. regarding "the rules of civilized warefare". I should think that these "rules" were unwritten; that they were simply common practice or "agreements" as it were (for example, the use of land mines was considered "ungentlemanly"). In any event, I wonder how these "rules" would apply to Sherman??
Unfortunately, I cannot quote the source (as I have forgotten) but I beleive that I read somewhere (or maybe heard a quote on Ken Burns' "The Civil War" series) that Sherman approved the stealing of personal property (or, at the very least, he turned a blind eye to it) and certainly the destruction of personal property, during the March to the Sea.
As to your statement about the things Sherman did to facilitate the end of the war, I agree....he did facilitate the end, but at what cost? Rape, robbery, murder, depriving citizens of food....I think he did more to deepen the hatred of Yankees and Union than anything. His destruction has kept the wounds of the Civil War open even today, so , yes, he is that bad. Gen. Grant is not nearly as hated today as Gen. Sherman is.
Albert Sailhorst
Scott's Battery


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So...here's the scoop. I'm a very mellow/ laid-back, kinda guy (naturally). I can enjoy a drink or two at a bar, but would usually rather be somewhere else. I'm an aspiring artist and enjoy being around liberal artsy types, but both of my closest friends have their M.B.A.'s...so who knows.
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The Pavilion is Tulsa's historic art deco arena, which is known for being the ideal venue for concerts, entertainment, horse shows, and fun for the whole family. With every seat, there is a perfect view, making the Pavilion the best choice in the heart of Tulsa.
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About Us
Established in 2000 by James D. Utterback, Rxcel Partners originally focused on supporting and serving the unmet needs of the Japanese Pharmaceutical and Biotechnology community in the United States.
Today, Rxcel supports pharmaceutical, biotechnology, medical device, and venture capital companies in their outsourcing strategies, including the efficient selection of quality, niche, CROs in key regions around the globe.
+ James Utterback
Founder & Chairman
"Jim" holds advanced degrees in Economics and Industrial Psychology. With over 30 years of experience in Life Sciences, he has held corporate staff roles in HR, Finance, and Corporate Communications as well as General Management/CEO positions in Pharma, CROs and start-up Device and IT product companies serving the clinical trials space. Jim has had P&L responsibility for businesses on 5 continents and has lived in the US, Europe and Africa.
+ David A. Dworaczyk PhD
Executive Partner
Dr. Dworaczyk has over 30-yrs of experience in the pharmaceutical, biotechnology, medical device and CRO industry where he has held senior level positions in R&D, Global Operations, Business Development (L&A/M&A), Clinical Development, Strategic Planning, R&D Portfolio Analysis, Project Management and Commercial Operations. He has done extensive work with numerous healthcare, Pharma, Biotech and CRO focused Venture and Private Equity investment groups assessing and evaluating investment opportunities from an operational, strategic and business potential perspective. Dr. Dworaczyk advanced degrees in Cell and Molecular Biology and in Leadership and Business Administration.
+ Patty Gebhardt
Sr Consultant- Technology and Life Sciences- Marketing and PR
As a strategic marketing expert, Patty excels in collaborating with healthcare and technology organizations to develop market-driven, differentiated products / services, brand preference, effective partnerships, and business results. Based in the Silicon Valley, Patty has spent the past twenty-five years helping both established global leaders and high growth, start-up organizations build focused, well integrated and efficient business and marketing plans. Patty's areas of expertise include: qualitative market research, competitive analysis, corporate/product/ service positioning strategies, brand identity, launch strategies, partner development, mergers and acquisitions, and accountability programs. Patty holds a Masters in Public Administration from Syracuse University. She has served on the Board of Directors for PowerOverCancer, a nonprofit organization based in Monterey, CA, since 2007.
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Senior Consultant- Biopharma Commercialization
Laura brings more than 17 years in sales and marketing roles in the biotech and pharmaceutical industry, with a proven ability to quickly analyze key business drivers and develop strategies to accomplish financial goals for pharmaceutical and biotech products. She has worked in both large pharma and small biotech companies, leading teams and planning launch strategies for multiple products. Laura has worked on three continents and many countries, enabling her to effectively communicate across cultural barriers and understand fundamental differences in marketing strategy across regions. Laura's core competencies include assessing product value propositions, building launch strategies and tactical plans, informing clinical trial planning and creating product acquisition or divestiture strategies and messages. She has particular expertise in identifying reimbursable differentiation throughout all phases of development, launch and lifecycle. Laura has an advanced degree in business administration.


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Last Updated: June 12, 2014
National City Tower Comes to Market
LOS ANGELES�Charles Dunn Co. lists the historic 93-unit, 12-story adaptive reuse building for $45 million with a cap rate of 4.7% on behalf of owners National City Towers LLC.
You can now be notified via email if this story is updated by clicking on the "Follow this Story" link. You must be a registered member to take advantage of this "members only" benefit.


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Honeymoon Over for Seahawks WRs
A not-so-funny thing happened to many of the Seattle Seahawks' young receivers on their way to another offseason of anonymity.
Sometime between the end of a 2007 season that saw Ben Obomanu, Courtney Taylor, Logan Payne and Jordan Kent get plenty of praise behind the scenes, and the beginning of the upcoming season, Seattle's young receivers became targets.
Not just of practice passes, but also of Mike Holmgren's ire.
Earlier this week, the Seahawks' intimidating head coach screamed at the mild-mannered Obomanu during a practice drill. A few minutes later, Holmgren barked at Payne.
The honeymoon is over for the Seahawks' young foursome.
"They could make a mistake last year, run a completely different play, and the coaches were like: 'That's OK. He doesn't know what he's doing,'" said receiver Nate Burleson, the 26-year-old veteran of the group. "But now, they're held at a higher standard. They're responsible to go out there and perform like a starter."
In the professional world, being the target of criticism is better than not being noticed at all. And so, in an odd way, the Seahawks' young receivers may well have arrived.
"You want that hands-on attention, especially from a guy like Coach Holmgren, who you respect," Taylor said. "When he's down my throat or someone else's throat, you look at it like: if he's not saying anything to you, you might be on your way out the door."
The current minicamp has gone on without starting receivers Bobby Engram, who is skipping the voluntary session while in a contract dispute, and Deion Branch, who will be out until at least September following knee surgery.
That leaves Burleson and a young receiving corps that includes eight other wideouts who have a combined 12 receptions at the NFL level. Obomanu, a third-year player who has appeared in just 13 professional games, is the second-most-experienced receiver at camp. Taylor, Payne and Kent, who spent most of last season practicing but not appearing in games, have had to grow up fast.
"It's a bigger transition than it was last year because more is expected of us," said Taylor, who was a rookie in 2007. "But you welcome it. That's why you're here: for those challenges. That's what it's all about."
The Seahawks expect the 35-year-old Engram to report to training camp next month and play during the regular season. Burleson is also penciled in as a starter.
Beyond that, the Seahawks have plenty of question marks.
So the buzz word at the current camp, with four receivers battling to open the regular season as the No. 3 wideout, is opportunity.
"It's a good opportunity for all of us," Obomanu said, "and at the same time, a good learning experience."
Added quarterback Matt Hasselbeck: "It's a great opportunity for these guys. I would say that they are doing their best to take advantage of it."
Based purely on experience, the 25-year-old Obomanu appears to have the inside track on the No. 3 receiver job. There's a chance he could even start, seeing as though the Seahawks would prefer to move Engram back to his natural role as a slot receiver.
But all four youngsters have shown enough flashes that the coaching staff has confidence in them to fill in the void left by Branch's injury and D.J. Hackett's free-agent defection to Carolina.
"They're very different," offensive coordinator Gil Haskell said. "What Jordan gives you, he's a tall, fast man. Logan, he's a tough (guy) who can catch the ball. Courtney Taylor might be the most skilled of them all. And Ben does it right every time.
"So you've got very different guys. They've just got to keep (improving)."
If the young receivers are searching for inspiration, they don't need to look far. Burleson was in a similar situation heading into his second NFL season with the Minnesota Vikings. After catching just 29 passes as a rookie, he broke out for 63 receptions and a team-high 1,006 yards in 2004.
"Everybody focused on Randy (Moss), and no one knew who Nate was," Burleson said. "And these guys are in the same position. Who knows who Logan Payne is? But he can come out and establish himself as a big-time player.
"These guys have a chance to establish themselves — overnight — as the next Wes Welker (the New England receiver who caught a league-best 112 passes last season). That's what they've got to understand."
Before that can happen, the Seahawks' young receivers have to endure a little tough love.
"That's pretty standard procedure," Payne said of the motivational tactics the coaches have taken with some of Seattle's inexperienced wideouts. "We're young, so (Holmgren is) going to try to over-coach us, get on us and make sure we're doing what we need to do. And we need that kind of stuff right now."
Burleson, for one, believes the youngsters will be up to the task.
"I've heard that the receiver position is a position of interest right now," Burleson said, "but honestly, I'm not worried. It's good to have young guys because they're hungry."
Who's got next?
A look at the Seattle Seahawks' receiving corps, which is in a state of transition:
D.J. Hackett 32 receptions in six games last season, but signed with Panthers
In limbo
Bobby Engram Skipping minicamps because of contract dispute
Deion Branch Knee injury will keep him out until September — at the earliest
Who's left
Nate Burleson Likely to return to starting lineup after solid 2007 season
Ben Obomanu Star of 2007 preseason is challenging for No. 3 role
Courtney Taylor Coaches love his potential, but he's got a lot to prove
Logan Payne Practice squad star in 2007 is challenging for playing time
Jordan Kent Former track/basketball star has speed and size, but is raw
Others: Michael Bumpus, Chas Gessner, Joel Filani and Trent Shelton
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Daily NewsView All News
SeatonCorp Revenue Up 21%
October 26 2011
SeatonCorp, a privately held Chicago-based staffing provider, reported third-quarter revenue rose 21 percent on a year-over-year basis. The company reported third-quarter revenue in its PeopleScout recruitment process outsourcing division rose 44 percent year over year.
SeatonCorp also reported its Staff Management vendor-on-premise staffing and managed service provider division added $62 million in incremental annualized revenue growth.
The company ranks No. 32 on Staffing Industry Analysts' list of largest U.S. staffing firms with 2010 revenue of $343 million.


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Mole, a small insect-eating mammal related to the shrew. It lives in underground burrows and is found throughout the Northern Hemisphere.The star-nosed mole has fleshy tentacles on the end of its nose.
The mole is covered by soft, velvety fur that ranges in color from gold to dark brown or black. It has tiny eyes and is almost completely blind. Its ears are small and are hidden beneath the fur. Moles are sensitive to underground vibrations but do not hear well above ground.
Unlike hedgehogs and shrews, moles spend almost their entire lives underground. They dig tunnels under the earth and live in them.
Their well-developed sense of touch makes up for their poor eyesight. Their sensitive whiskers and body hair help guide moles through the darkness. The tip of a mole's snout has tiny bumps that feel every object it comes in contact with. Moles also have excellent senses of smell and hearing.
An adult mole is 5 1/2 to 9 inches (14 to 23 cm) long and weighs from 1.2 to 6 ounces (34 to 170 g), depending on the species. It has a cylindrical body and a short tail that is either bare or hairy. The tubular, bare nose is used in digging tunnels and extends beyond the mouth. The legs are short and stout. The spadelike forefeet have sharp claws and the palms are turned outward, which aids in digging.
Moles are solitary creatures. They spend most of their time in a network of tunnels. The tunnels, usually less than six inches (15 cm) below the surface, leave ridges of earth, called molehills, above ground. Some species dig a second set of tunnels, up to 18 inches (45 cm) below ground, for nesting. In digging, the mole first loosens the earth with its snout and then thrusts its forefeet forward, pushing the earth out to the sides.
The mole feeds primarily on insects, worms, and vegetation; some species also eat crustaceans, fish, and mice. The mole has such a high metabolic rate that it must eat two or three times its own weight in food each day; it cannot go without food for more than 10 or 12 hours.
Although they eat insects, moles prefer big, juicy earthworms. Moles like them so much that some moles store extra worms away for future meals in special storage chambers. A scientist uncovered one storage chamber containing 1,280 earthworms and some grubs. The total weight of the stored food was about 4.4 pounds (2 kilograms).
Moles also like to feed on larger animals, such as lizards and mice. Like shrews, moles must eat an enormous amount of food every day to stay alive.
Moles mate in late winter or early spring. The female usually builds a grassy nest in a deep tunnel; however, the females of some species build a grassy nest above ground. Three to seven young are born in early spring. They are independent at three weeks and mature at 10 months. Moles live four to five years.
The mole is considered a nuisance because the ridges and mounds made by its tunneling can ruin the appearance of a lawn or golf course. On the other hand, moles help control harmful insects. Mole skins, which are warm and waterproof, are used to make fur wraps. Hundreds of skins are needed for a single garment. Moleskin is a fabric that resembles the mole's coat.
Moles are expert diggers. And moles love to tunnel through lawns and gardens. They create molehills on the surface as they go. Moles dig some of their tunnels close to the surface where insects and worms can be found. Their digging cuts off root systems of grass and plants. One mole can ruin a lawn, a garden, or a flower bed.
The bodies of moles are built for digging. The large forelegs have flat, shovellike feet that turn outward. At the end of each foot are large, broad claws or nails, perfect for scooping out dirt. The heads and backsides are tapered, starting wide and getting narrow. This allows moles to move easily through the earth.
It's very dark as you might imagine. But if you could see, here is what you would find. At the center of a mole's network of tunnels is a nest. This is where it lives and sleeps. Some distance away from the main tunnels may be a room where a female mole cares for her young.
The tunnels branch out from the nest. About every four hours, a mole can complete a tour through the tunnels—looking for worms, spiders, and insect larvae (Lahr vee) to eat.


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By Ryan Clark | April 29, 2011 | Comments
WWE held their annual stockholder's meeting today in Stamford, CT. During the meeting, the floor was opened for questions from stockholders:
Vince McMahon was asked about WWE's relationship with the Rock, specifically whether the company's new deal with him was cost-effective, since they obviously were having to pay a high premium for someone of his current stature. McMahon responded that he and Rock are working on a handshake deal and that is "how they have operated for a long, long time." McMahon said it's all based on increased value. He said that if there is a higher percentage in terms of growth in terms of what they would "normally expect", Rock is "going to get a piece of that". McMahon said their net was going to be much higher and he and Rock were proud of that.
How the other WWE PPV events were doing compared to the past was brought up. McMahon said the PPV business is down but he's hoping that Wrestlemania's PPV numbers will be a kickoff for a new year in terms of numbers for the company's PPV events.
McMahon was asked whether Smackdown had retained its audience in the transition from MyNetworkTV to SyFy, McMahon said that the show has just about the same penetration now and they are very happy with SyFy, stating that they have "been the number one show on the network" since they've debuted there. He said if there's been any change in distribution, it's offset by the advantages of being under the NBC Universal family through promotion. McMahon noted that the syndicated network system, such as MyNetworkTV, is in trouble. Being on SyFy gives them longevity and provides them with non-preemptive status (i.e. they won't be bounced out their timeslot), which is "so important" to the company. He explained how they would often be bounced for sports programming on MyNetwork and the audience would be diluted when they were moved out of the slots.
Triple H's international development of talent was brought up, as was the idea of launching other groups in other countries with locally cultivated stars. McMahon said that there will be a more aggressive attempt to bring in international talents and grooming them, but they need to become stars here, because if they are, they will be stars elsewhere. McMahon said that with Miz, Alberto Del Rio and Sin Cara, you are seeing talents on the "fast track" to becoming the new "Rocks and John Cena of the day."
The company's intellectual property was brought up and McMahon was asked what the biggest problem in terms of protecting it is. McMahon said piracy and that they have a very aggressive group that handles that aspect. They noted piracy is incredibly difficult outside the U.S. as well as inside of the country. There's no silver bullet that can stop it. The hope is that as countries become more developed as markets, they will help crack down on it. McMahon noted that they just went on the air in Russia and Brazil "but we've been there a long time." He said the best way to fight piracy in other countries was to actually bring the content to the countries.
In regard to expanding and re-branding the company, McMahon was asked whether he had learned anything from past failures like the XFL and the WWE restaurant. McMahon said that when you think about it, none of those ventures have used the core competencies of the company as their current plans. He said that they have no interest in going back into football or opening a restaurant, noting that they "had to inherit that business right before we went public." He also noted there was no interest in returning to bodybuilding. He said they aren't looking to invest into anything like that but acquisitions that they can "fit what we do" and things "we can, in essence, flip the model on". McMahon explained that they can acquire the license of an property and then run it through the same system of distributions, context and partners that they put the WWE property through.
2 Of 3 Falls Matches On WWE NXT DVD, Cesaro And Tyson Kidd Photo, Crazy New DDP Yoga Promo
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Daniel Bryan's Trainer Talks About Working With Shawn Michaels, His Attitude, Training Bryan, More
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Raven On Why He Recently Sued WWE, Drinking With Shane McMahon, Impressing Vince McMahon, More
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Jim Ross Blogs About Stars WWE Can Bring In For WrestleMania 32, Daniel Bryan Not Getting Cleared


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Retroelements are a large class of genetic elements that multiply by the reverse transcription of an RNA intermediate. The resulting cDNA is incorporated into the genome of host cells. In eukaryotes, the wide-spread success of long terminal repeat (LTR)-containing retroelements has led to replication mechanisms that are conserved among diverse families of retrotransposons and retroviruses. The medical importance of retroviruses such as HIV has intensified the need to understand the molecular details of the mechanisms responsible for the propagation of LTR-retroelements. Since LTR-retrotransposons exist in yeast, the powerful techniques of yeast genetics can be applied to answer basic questions about the function of LTR-retroelements. In turn, this fundamental information may identify new antiviral targets or stradegies that can be used to combat the spread of retroviruses such as HIV.
The similarities of retrotransposons to retroviruses includes the presence of two long terminal repeats (LTRs) and open reading frames (ORFs) with coding sequences homologous to retroviral protease (PR), reverse transcriptase (RT), and integrase (IN). The first step in the transposition pathway as shown in Figure 1 is synthesis of a full-length mRNA with sequence that begins in the 5' LTR and terminates in the 3' LTR. This is directly analogous to the initial step in retrovirus particle formation. Retroviral and retrotransposon mRNAs are translated into proteins that assemble along with the mRNA into large particle structures. Both retroviral and retrotransposon particles undergo a maturation process that includes the proteolytic processing of precursor proteins and the reverse transcription of the mRNA. Retrotransposon particles complete transposition by simply inserting their DNA into the genome of the original host cell. Because each step in retrotransposition is directly related to a retrovirus process, results from the investigation of yeast retrotransposition are relevant to aspects of retrovirus behavior.
Figure 1. Synthesis of a full-length mRNA with sequence that begins in the 5' LTR and terminates in the 3' LRT.
The retrotransposon we study is the Tf1 element of the fission yeast Schizosaccharomyces pombe. The transposon is 5 kb and contains a single ORF with coding sequences for Gag, PR, RT, and IN (5). We have previously demonstrated using an in vivo assay that uses a neo-marked version of Tf1, that at least one of our cloned copies is active and can transpose at a significant frequency (3, 4). Figure 2 shows that cells that contain transposition events grow readily on the drug G418. This system allows us to use a battery of powerful molecular and genetic techniques to identify and characterize factors that contribute to the transposition process. The following are summaries of our current research projects.
Figure 2. Cells containing transposition events grow readily on the drug G418.
For more information, see the Section on Eukaryotic Transposable Elements' current research projects:
The IN of Tf1 contains a Zn finger-like motif and a DDE motif, two sequences typical of retroelement integrases (Levin, Weaver et al. 19909). Further analysis of its sequence revealed that Tf1 IN also contains a chromodomain (CHD) at its C-terminus (Malik and Eickbush 199910). CHDs are present in chromatin modifying proteins such as Hp1 and interact directly with histone proteins with specific post-translational modifications. The presence of a CHD suggests Tf1 IN may interact directly with specific nucleosomes during integration. This is particularly interesting in the case of Tf1 because such an interaction may play a role in the insertion preference of Pol II promoters. In these first studies of the CHD, we examined its role in the catalytic activities of IN.
The full-length IN and IN lacking the CHD (CH-) were expressed in bacteria and purified extensively with cobalt agarose and heparin sepharose. To measure catalytic activity and optimize reaction conditions we used the disintegration assay. This assay measures the reverse of integration and was used initially because the substrate sequence and structural requirements are less stringent than for the forward reaction. The model substrate consisted of a 76 nt oligonucleotide that mimics the end of the LTR attached to the bottom strand of a target. A 20 nt oligonucleotide is added to mimic the top strand of the target. IN activity will catalyze a transesterification reaction that joints the 20 nt top substrate to the bottom strand of the target. This converts the 20 nt DNA into a 63 nt product, a change that can be readily detected on polyacrylamide gels. These experiments revealed that IN possessed substantial levels of catalytic activity. Surprisingly, the CH- protein was significantly more active then the full length IN.
To test the IN proteins for their ability to catalyze the forward reaction, a 24 nt double-stranded DNA was used as the substrate in strand transfer assays. The DNA contained the sequence of either the U3 or U5 ends of the Tf1 LTR and served both as donor and target. IN exhibited substantial strand transfer activity as indicated by the production of higher molecular weight DNAs. Once again, the IN lacking the CHD, CH-, had substantially more activity than the full-length IN. These data substantiated that Tf1 IN was active as a recombinant protein and that the CHD functioned to inhibit this activity.
The strand transfer activities of retrovirus and retrotransposon INs require that the highly conserved "CA" dinucleotide be present at the 3' end of the donor substrate. We tested whether the IN of Tf1 had this same requirement for "CA" and whether the CHD contributed to this specificity. IN and CH- were tested for whether each of the last three nucleotides of the LTRs, "ACA" was necessary for strand transfer. All the modifications introduced in the sequence led to a dramatic reduction of the strand transfer activity of IN. In sharp contrast to IN, CH- retained high levels of activity with most of the substituted substrates. For example CH- retained 50% of its activity with the substrate that had a minus one transition (AtA) whereas the full length IN had just 1% of its activity with this substrate. This large difference in the specificity of the two related proteins shows that IN was far more stringent than the CH- counterpart in selecting the DNA donor with the correct 3' end.Both contributions of the CHD, limiting IN activity and increasing sequence specificity, may result from a single mechanism that restricts access to the active site of IN. One intriguing hypothesis is that the CHD restricts IN activity until it interacts with histone H3 or some other factor and it is this interaction that relieves the inhibitory function of the CHD and allows integration to occur.
The INs of retroviruses have a 3' processing activity that removes the two or three nucleotides 3' of the "CA" so that strand transfer can occur. The primer for the minus strand of Tf1 is positioned adjacent to the LTR allowing the cDNA to terminate with the "CA" that is necessary for strand transfer. Thus it was predicted that no processing activity would exist (Levin 19957; Levin 19968). However, sequences of Tf1 cDNA extracted from particles revealed the surprising result that 85% of the 3' ends had one or more untemplated nucleotides (Atwood-Moore, Ejebe et al. 20051). Because these nucleotides are expected to block strand transfer, we tested whether Tf1 IN had a processing activity. In this experiment, the 5'-end labeled substrates contained nucleotides positioned 3' to the critical "CA". The data from these experiments showed that IN did have processing activity capable of removing as many as 5 nucleotides from the 3' end of the substrate. The processing activity could in theory remove the 3' nontemplated nucleotides and allow the bulk of the cDNA to participate in integration. This model raises the question why would nontemplated nucleotides be added just so they could be removed by IN. One possibility is that the nontemplated nucleotides could protect the conserved "CA" from attack by nonspecific 3' exonucleases.
The C-terminal domains in the INs of LTR-retrotransposons and retroviruses are not well conserved. However, close examination of C-termini did identify one motif that exists in a wide variety of INs (Malik and Eickbush 199910). This module termed the GPY/F motif is present in the INs of a diverse set of LTR-retrotransposons in the Metaviridae family (formally Ty3/gypsy) and in the gamma class of retroviruses (Fig. 1A) (Malik and Eickbush 199910; Jern, Sperber et al. 2005 5). The function of this motif has not been studied. The IN of Tf1, as a recombinant protein, is highly soluble and possesses robust catalytic activity (Hizi and Levin 2005 4). These properties motivated us to consider the IN of Tf1 as a potential model to study the function of the GPY/F domain.
Since little was known about the structure of Tf1 IN, our initial experiments tested whether it was sufficiently similar to other INs to serve as a model. IN purified from bacteria was subjected to partial proteolysis with trypsin to determine whether it possessed the three domain architecture found in other INs. The N-terminal amino-acid sequences of the protein fragments determined the cleavages occurred at amino acids 110 and 354. This confirmed IN was composed of N-terminal, central core, and C-terminal domains (Fig. 1B). When the full Tf1 IN is aligned with other INs its conserved residues and the size of its domains closely resembles the IN of Moloney murine leukemia virus (M-MuLV) (Fig. 1B). The cleavage between the central and C-terminal domains occurred in the middle of the GPY/F motif. This indicated the GPY/F region assembles into two stable segments split by less structured residues.
Figure 3. The GPY/F motif of IN. A.
An alignment of INs from the Metavirus family of transposons and the Gamma family of retroviruses shows the conserved residues of the motif (yellow). B. A scaled diagram of INs showing the three domain architecture reveals similarities between the INs of Tf1 and M-MuLV.
To validate whether the domains of Tf1 IN possessed the functions associated with the domains of other INs, and to study the function of the GPY/F motif, a series of recombinant proteins consisting of different sections of Tf1 IN were expressed in bacteria and purified (Fig 2). The central core domain of HIV-1 IN contains the catalytic module that is sufficient to support strand cleavage and joining as measured with the disintegration assay. However, in the IN of M-MuLV the domains are larger, and in this case both the central and C-terminal domains are necessary for catalytic activity. We tested which portions of Tf1 IN were required for catalytic activity using the same disintegration assay in the previous section. The central domain by itself lacked activity. Sequential deletions revealed the N-terminal domain and the GPY/F motif were necessary for activity.
Figure 4. Section of Tf1 IN produced as recombinant proteins.
Figure 5. Gel filtration of IN proteins revealed that the GPY/F fragment formed multimers.
In solution the INs of HIV-1, M-MuLV, and avian sarcoma virus (ASV) form a dimer-tetramer equilibrium. In initial experiments to test the IN of Tf1 for the propensity to multimerize we tested full-length IN for interactions with the individual portions of the protein. Using a precipitation procedure and our recombinant proteins, we found that the N-terminal domain, the central core, and the 71 amino acid GPY/F fragment all bound to the full-length IN. To test directly for stable multimers we performed gel filtration with superdex 200. In a buffer of 50 mM HEPES, pH 7.5, 0.5 M NaCl, and 1% (v/v) glycerol, IN at 1 mg/ml eluted as a single peak with an observed molecular weight of 126.5 kDa, the size predicted for a dimer. The central core by itself was also observed to form a stable dimer. This ability of the central core and the full-length proteins to dimerize was typical of other INs.
To investigate the contribution of the C-terminal domains to the multimerization of IN, the GPY/F fragment and the CHD were subjected to gel filtration with superdex 75 (Fig. 3A). The CHD had an estimated molecular weight of 11.7 kDa, indicating it was a monomer. The profile produced by the GPY/F fragment included three major peaks (Fig. 3B). The apparent sizes of these species were monomer, dimer, and trimer. To test whether the GPF residues in the center of the motif contributed to multimerization, single amino acid substitutions were generated. Both substitutions, GPF to APF and GPF to GAF completely disrupted multimerization of the GPY/F fragment (Figs. 3C and 3D). These data indicate that the GPY/F residues play a central role in promoting multimerization. In separate experiments to test for multimers we subjected the GPY/F fragment to the chemical cross-linker dithiobis succinimidyl propionate. Gel electrophoresis of the cross-linked sample indicated the protein was in an equilibrium of monomers, dimers, trimers and tetramers.
The C-terminal domains of INs are known to bind DNA without sequence specificity. To map which sections of Tf1 IN interact with DNA, each of the individual domains was assayed for DNA binding. Labeled oligonucleotides were mixed with the individual domains and the mixtures were cross-linked by UV. The full-length IN, CH-,, core, and the GPY/F fragment had substantial DNA binding activity. These DNA binding activities correspond well to what has been described for other INs. Interestingly, CH- bound significantly more DNA than the full-length IN. This indicated that the inhibitory activity of the CHD may act by blocking DNA binding.
In additional experiments the single amino acid substitutions in the GPF residues did not reduce DNA binding. This result indicates that other sequences in the GPY/F fragment mediated the DNA binding activity and the contribution of the GPF residues in the GPY/F fragment appears to be specific for promoting multimerization.
The contribution to catalysis of the GP residues was tested by generating recombinant IN with the substitutions GPF to APF and GPF to GAF. Both of these mutations abolished the strand transfer and disintegration activities. This requirement of the GPF residues for catalysis together with their contribution to multimerization indicated that the principle function of the GPY/F motif was to promote multimerization of the C-terminus. Our finding that the GPF to APF and GPF to GAF mutations did not reduce DNA binding indicated that multimerization was not required for DNA binding. The wide-spread conservation of the GPY/F motif supports the conclusion that these amino acids play a critical role in the function of IN.
Many transposable elements protect the coding capacity of their host by directing integration to nonessential regions of the genome. The preference of Tf1 for integrating upstream of genes is a mechanism that protects the coding sequences of S. pombe. This preference may be due to interactions of IN with chromatin factors. This possibility is supported by the presence in the IN of a CHD that may bind histones with specific modifications (Malik and Eickbush 1999 10). Alternatively, the integration of Tf1 upstream of ORFs could be due to interaction with chromatin remodeling complexes or transcription factors. To identify what determinants specify the positions of integration we developed a plasmid-based targeting assay in cells induced for Tf1 transposition.
To identify what factors determine the insertion sites of Tf1 integration we developed a plasmid based targeting assay that measured the integration activity of specific sequences. A strain of S. pombe was generated that contained a plasmid for the expression of Tf1 and a plasmid for target sequences. Tf1 was marked with neo so that integration would result in resistance to G418. After transcription of Tf1-neo was induced, the expression plasmid was removed by counter selection and cells with insertion events were selected on medium containing G418. Target plasmids extracted from individual patches were introduced into bacteria by selecting for resistance to ampicillin. Due to the presence of neo in Tf1, target plasmids with insertions resulted in colonies that were resistant to both ampicillin and kanamycin. These plasmids were readily identified and the position of their insertions sequenced.
Figure 6. Integration pattern of Tf1 into a target plasmid containing ade6.
To test the integration preferences of Tf1 a target plasmid was created that contained a potential target consisting of the ORF of ade6, its upstream intergenic region, and a portion of the adjacent ORF, bub1 (Fig. 1). The ade6 and bub1 genes were in divergent orientation so the 405 bp integenic sequence contained two promoters. This particular segment of the pombe genome contains chromatin structure that is maintained when placed on a plasmid. The only other portion of the target plasmid that contained a promoter was LEU2d, a selectable gene from S. cerevisiae that has a damaged promoter. The target plasmid was introduced into a strain of S. pombe and individual patches of cells were induced for the expression of Tf1-neo. No selection was placed on the function of ade6. The patches were then replica printed to medium containing G418 to select for cells with integration events. Target plasmids were isolated by preparing DNA from each G418R patch and electroporating the DNA into bacteria. Colonies resistant to ampicillin and kanamycin contained target plasmids with insertions of Tf1-neo. Of 43 independent insertions of Tf1 isolated in the plasmid, 41 (95%) occurred in the intergenic region between bub1 and ade6 (Fig. 1). These results demonstrated that the preference of Tf1 for integration upstream of ORFs was reconstituted within the context of a plasmid. All 41 insertions in the intergenic region occurred within a 160 bp window. Interestingly, the insertions clustered at specific nucleotides where no preferences for orientation were observed.
Figure 7. Integration frequency and target efficiency of plasmids with deletions in ade6. The 160 bp target window (gray line) in the promoter of ade6 is the only sequence required for Tf1 integration.
To identify which sections of bub1-ade6 were important for targeted integration, we made a series of deletions and tested their impact on the frequency of integration (Fig. 2). These data revealed that the 160 bp target window was the only portion of bub1-ade6 that was important for efficient integration. To test whether the insertion window was sufficient to be an integration target, a plasmid was created that contained just the 160 bp window. These experiments showed that just 173 bp of the promoter sequence functioned as a highly specific target for integration.
The targeting of Tf1 to the region of the ade6 and bub1 promoters suggests that the transcription of these two genes may play a critical role in integration. This possibility was tested by comparing the amounts of mRNA produced by the deletion plasmids to their efficiency of targeted integration. Two deletions in the promoter sequences exhibited sharp reductions in the transcription from both promoters and yet both plasmids supported efficient targeting. This indicates that the transcription activity of the promoters per se did not play an important role in targeted integration.The result that active transcription was not important for efficient integration into the promoters of bub1-ade6 suggests that features such as DNA binding proteins may be recognized by Tf1. To determine whether nucleosomes or DNA-bound transcription factors were positioned at integration sites we mapped the chromatin at the promoters of bub1-ade6 with micrococcal nuclease. These experiments revealed four supersensitive sites clustered together within the intergenic sequence. The intensity of these bands and their close spacing within the promoter region suggests they were created by transcription factors bound to regulatory sequences. Of the five major sites of insertion, four corresponded closely with positions of micrococcal sensitivity. This close association suggests the promoter binding proteins responsible for the micrococcal sites played a role in recruiting IN to the sites of integration. This is consistent with the finding that 160 bp of the bub1-ade6 promoters was the only sequence required for targeted integration. Unfortunately, the transcription factors that bind the promoters of bub1-ade6 are unknown. As a result, we chose to study the integration activity of a promoter for which the transcription factors are known.
To test whether Tf1 integration was directed by transcription factors, we studied the insertion activity of a promoter that has been characterized extensively. The promoter of fbp1 is highly regulated and contains an upstream activating sequence (UAS1) that consists of an eight bp binding site for the transcription activator Atf1p (Neely and Hoffman 2000 11). In addition, UAS2 consists of a short binding sequence for the transcription activator Rst2p as well as other factors (Neely and Hoffman 200011); Higuchi, Watanabe et al. 20023).We tested whether the promoter of fbp1 was a target for Tf1 integration using the target plasmid assay. Eighty-six percent of the insertions in the plasmid occurred within the promoter region of fbp1. Thirteen ( 62%) of the 21 insertions isolated in the plasmid occurred at the two dominant positions, 4923 and 4933 (Fig. 3A). These positions were just 30 and 40 bp downstream of UAS1. The lack of any insertions in the open reading frame and the clustering of inserts upstream of the open reading frame indicated that the insertions were specific for the promoter of fbp1. The role of transcription factors in integration was further supported by the concentration of integrations that occurred adjacent to the binding site of Atf1p at UAS1.
Figure 8. Integration sites in the fbp1 promoter. A. The wild type promoter has two dominant insertion sites. B. The eight nt mutation of UAS1 reduced integration at the dominant sites by 9-fold.
The integration activity of fbp1 and the published information about UAS1 allowed us to ask whether the clustering of inserts in the promoter region was the result of UAS1 function. We changed the sequence of all eight nucleotides in UAS1 of the fbp1 plasmid and used the target assay to ask whether the pattern of integration was changed. In the plasmid with the mutated UAS1, just three of 42 insertions (7%) occurred at positions 4923 and 4933 (Fig. 3B). Thus, the mutated UAS1 resulted in a 9-fold reduction in integration at the two dominant positions of insertion. The defect in the ability of UAS1 to be recognized by Tf1 allowed the secondary targets 5183 and 5149 to become major sites of integration. This result demonstrated that a functional binding site for the transcription factor Aft1p plays an important role in the targeting of Tf1 integration to the two major insertion sites in the fbp1 promoter.
The integration of Tf1 in the promoter of fbp1 may result from a tethering of IN to UAS1 by Aft1p. An interaction of Atf1p with IN could be direct or through proteins that bind Aft1p, such as the coactivator Pcr1p. Immunoprecipitation of Atf1p from cell extracts revealed that IN was in a complex with Atf1p. Further experiments tested whether Atf1p itself contributed to integration in the promoter of fbp1. Results from plasmid target experiments revealed that in the strain lacking Atf1p, insertions were no longer directed to the fbp1 promoter. Together, these results indicate that Atf1p mediates integration of Tf1 in fbp1 by tethering IN to UAS1. Whether integration at other promoters is due to Aft1p or other transcription factors remains to be tested.
The relationship between transposable elements and their hosts allows for integration of the transposon that does not impair the fitness of the host. The insertion of Tf1-neo introduces approximately 6 kb of DNA into the promoter region of genes. This form of DNA damage might be expected to disrupt promoter function. For example, one deletion we made in the promoter of ade6 revealed key promoter elements were upstream of all the major sites of integration. We tested whether the integration of Tf1-neo at the primary site of insertion, nucleotide 4573, disrupted the function of the ade6 promoter. Target plasmids with Tf1-neo insertions were introduced into S. pombe and the levels of ade6 mRNA were measured on RNA blots. The ade6 mRNA was quantified relative to the amounts of actin mRNA. The copy number of the plasmids in each transformant was determined on DNA blots and these values were used to adjust the levels of ade6 mRNA. Surprisingly, insertion of Tf1-neo actually stimulated ade6 transcription. Insertion in the left orientation at nucleotide 4573 increased ade6 mRNA by 2.1-fold while insertion in the right orientation increased ade6 expression by 3.6-fold. Even though the insertions were downstream of essential promoter elements, ade6 expression was not reduced. The absence of defects in the expression of ade6 together with the increases observed suggests the intriguing possibility that Tf1 carries promoter elements that are capable of repairing the promoters disrupted by the transposon.
We tested directly whether Tf1 was capable of activating a damaged version of the ade6 promoter that lacked key promoter elements. A deletion that removed the sequences between the target site 4573 and the ATG of bub1 reduced levels of ade6 mRNA 4.3-fold. An identical construct except that it contained Tf1-neo inserted at nucleotide 4573 produced 7.2 and 2.9 times more ade6 mRNA depending on the orientation of Tf1-neo. These data demonstrate that Tf1-neo introduced promoter elements that substituted for the key transcription components located upstream of nucleotide 4573.
The insertion of Tf1 sequences could cause the target promoters to adapt the regulatory properties of Tf1 transcription. If Tf1 expression is similar to a closely related element Tf2, conditions of oxidative stress would induce a wave of transposition that in turn could cause cellular genes to become regulated by stress. This process could provide a selective advantage for the host by inducing genes that could increase survival. This would be an evolutionary strategy for creating populations of cells expressing different genes in response to stress that would improve the chances of survival for the species.
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The state of Ohio might want to give some of its moisture to us.
The governor has declared a state of emergency in nine counties as part of the state pushes through its worst flooding in nearly 100 years.
Authorities are shutting down schools, streets and at least 500 people have been evacuated.
At least 22 people were killed by two storm systems, one that's thrashing the upper Midwest and the other that soaked Texas, Oklahoma and Missouri.
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Taking a look at the chart I made below you can see quite a few positive indicators for Orbite. Our symmetrical triangle also indicates a breakout in the near future.
Trading above the 200dma (red line) - POSITIVE
50d SMA (blue line) in an upward trend - POSITIVE
SP coming very close to touching the bottom bollinger band indicating the stock is oversold - POSITIVE
RSI of 37.4. Getting close to the "magical" number of 30 indicating a buy signal - POSTIVE


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MacGyver: Season 2, Episode 8
Eagles (17 Nov. 1986)
Ratings: 7.8/10 from 132 users
Reviews: write review
Two rare golden eagles facing extinction are helped by MacGyver. He must simultaneously confront the poacher trying to kill the eagles and eliminate MacGyver.
Director:Paul Krasny
Writers:Lee David Zlotoff (created by), George Lee Marshall
Stars:Richard Dean Anderson, Dana Elcar, Julie Cobb | See full cast and crew »


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The Royal Gorge is a canyon on the Arkansas River near Cañon City, Colorado and is often called the "Grand Canyon of the Arkansas." With a width of 40-50 feet at its base, a few hundred feet at its top, and a depth of 1,200 feet in places, the 10-mile-long canyon is a narrow, steep gorge through the granite of Fremont Peak.


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I keep finding these things whenever I weed the garden.
Very good, but a little blurry around the eye sockets. Still, a very good entry
Well sorry dudes, its a fake.. look at the graphic and lighting errors around the teeth.
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Kyle Bonner
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Bond was set at $1 million for an 18-year-old man charged with murder in connection with a March fatal shooting in Walnut Hills.
Kyle Bonner of College Hill was arraigned in Hamilton County Municipal Court Friday in front of Judge Lisa Allen. Bonner was assigned a public ...


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Grilling blogger and cookbook author Chris Grove is t he author of "The Kamado Smoker & Grill Cookbook". For more on his recipes visit www.nibblemethis.com
1 large (11-inch x 9-inch x 2 3/8-inch) aluminum foil baking pan
2 to 3 cans (12 ounces each) beer
2 tablespoons butter
1 medium yellow or white onion, sliced
Johnsonville® Brats
1. Put the pan right on the grill, being careful not to singe your knuckle hair. Pour in the beer, and add the butter and onions.
2. Grill your Johnsonville Brats to a juicy, golden-brown perfection.
3. Serve immediately and place any remaining brats into the steaming hot tub.
Mango Mustard Slaw Dressing
2 Tbs. El Diablo Mango Mustard
2 Tbs. Mayonnaise
1 Tbs. Apple Cider Vinegar
1 tsp. ground black pepper
1 tsp. kosher salt
1 tsp. sugar
Whisk all ingredients together. Makes enough for 5 cups of shredded slaw veggies or a bab og slaw mix from the grocery store.
Chris Grove is a grilling blogger at www.nibblemethis.com
His new book "The Kamado Smoker and Grill Cookbook" (Ulysses Press) is on sale now.


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UPDATE: On May 16, 2012, the CDC announced acceptance of the ACCLPP's recommendation to replace "level of concern" for lead poisoning with a new reference value of 5 mcg/dL. To learn more, read the CDC's response.
Citing new data that lead causes harm to children at levels lower than previously thought, an advisory committee for the Centers for Disease Control and Prevention (CDC) urges that public health efforts focus on preventing lead exposure, a policy called "primary prevention," and also now recommends that the cutoff value for blood lead levels be lowered from 10 micrograms per deciliter (mcg/dL) to 5 mcg/dL. The January 4 report by the Advisory Committee on Childhood Lead Poisoning Prevention (ACCLPP) notes that even very low levels of lead can have long-term adverse health effects on children.
In emphasizing primary prevention, the committee aims to shift the focus of efforts to prevent lead exposure before children are harmed, rather than reacting to exposure that has already occurred. Their recommendations list ways that exposure from lead-tainted soil, water, dust and paint can be eliminated. For example, children should not be present when lead in old paint is exposed, such as when sanding or peeling paint, in homes built prior to 1978. They also advise prenatal counseling of mothers when possible. An assessment of the child's environment for potential sources of lead is encouraged even before screening for lead in the blood. For details from the CDC on how to prevent lead exposure, see web page Lead: Prevention Tips.
The recommendations in the report are based on a growing body of evidence from several studies that demonstrate harmful effects in children with blood lead levels less than the current limit of 10 mcg/dL. Up to now, this limit has been referred to as the "level of concern," but the ACCLPP recommends dropping that designation and putting the emphasis on prevention, noting there is no threshold level at which exposure to lead is considered safe. When testing blood lead levels, the committee favors using a lower 5 mcg/dL cutoff to notify public health officials when a child has been exposed to lead at a level greater than the general population (greater than 97.5% of all children in the U.S.).
A level above the established cutoff alerts doctors and public health officials to institute measures to reduce further harm. These actions usually include identifying the environmental source of the lead so as to eliminate it or minimize further exposure, providing education about lead poisoning, and establishing frequent testing and closer medical monitoring of those affected.
Over the years that U.S. children have been screened for lead, the limit has gradually been lowered as new evidence accumulates on the health problems from minute amounts, such as impaired development, lower IQ, learning disabilities, and behavioral disorders. Once a child has been affected, the problems are essentially irreversible. The committee's report also points to studies that are currently evaluating lead's influence on the health of the cardiovascular system and hormones (endocrine system). Young children are especially vulnerable to lead because they grow so quickly and are more likely to ingest or absorb lead from their environment.
Screening children for elevated blood levels has long been part of the strategy for minimizing their exposure to lead and preventing lead poisoning. Increased lead in the body does not always result in any immediate or obvious signs or symptoms, so an elevated level is often only detected through testing. Depending on children's risk of exposure, doctors may test them between 1 and 2 years old and again between 3 and 6 years old.
The ACCLPP recognizes the challenges that laboratories face in testing for lead at the new lower level. There is normally a certain amount of variation in results even when repeating the test on the same sample, and this variation can have greater impact when measuring very small amounts. Laboratories will have to ensure that the lead test is as precise and accurate as possible. To help ensure accuracy, the report states that all positive results are to be confirmed with repeat testing, and that results greater than 45 mcg/dL, or symptoms of lead poisoning, must be confirmed within 48 hours. Because the test can be contaminated from lead on a child's finger, a positive result from a fingerstick sample must be confirmed using a blood sample collected from a vein in the arm. Once lead exposure has been confirmed, activities to prevent further harm are set in motion.
Lowering the testing cutoff would increase the number of children identified as having lead exposure. Approximately 450,000 children in the U.S. have lead levels above the recommended revised limit, says the ACCLPP, compared to the 250,000 children estimated by the CDC using the current cutoff value. The lower threshold could impact efforts to address this public health problem, improving detection so that actions can be taken sooner.
Though the ACCLPP has proposed changes to the policy on lead along with the 5 mcg/dL cutoff value for lead testing, it may be some time before they are put into practice. The committee notes that the CDC and the Department of Health and Human Services will perform an internal review of the full report and will consider all the recommendations before deciding which to formally adopt and determining how those approved will be implemented.
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Tests: Lead
Conditions: Lead Poisoning
Screening: Infants - Lead poisoning; Children - Lead poisoning
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Article Sources
NOTE: This article is based on research that utilizes the sources cited here as well as the collective experience of the Lab Tests Online Editorial Review Board. This article is periodically reviewed by the Editorial Board and may be updated as a result of the review. Any new sources cited will be added to the list and distinguished from the original sources used. To access online sources, copy and paste the URL into your browser.
(January 4, 2012) Report of the Advisory Committee on Childhood Lead Poisoning Preventions of the Centers for Disease Control and Prevention, Low Level Lead Exposure Harms Children: A Renewed Call for Primary Prevention. PDF available for download at http://www.cdc.gov/nceh/lead/ACCLPP/Final_Document_011212.pdf through http://www.cdc.gov. Accessed Jan 31, 2012.
(June 1, 2009) Centers for Disease Control and Prevention. Lead. Available online at http://www.cdc.gov/nceh/lead/ through http://www.cdc.gov. Accessed Jan 31, 2012.


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EdgeFuse: A Pinterest For Making Weapons
Because of defense budget cutbacks, military contractors are looking for new money-saving innovations. So defense firm Mav6 is unrolling EdgeFuse, a social-media-inspired tool to connect the defense industry to soliders.
It was inevitable that defense contractors would embrace social media for R&D sooner or later. Mav6, a smallish aerospace outfit, is promoting a new platform called EdgeFuse that lets defense contractors, military personnel, and government employees trade relevant tweets and Instagram photos via smartphone—in short, a hybrid of Delicious and Pinterest. Will social networking build better weapons?
Mav6's senior vice president Gregg Sypeck seems to think so. Sypeck, a former IBM and Fujitsu consultant, works at a firm heavily staffed by Blackwater alums and military personnel with tech backgrounds. In an interview with Co.Exist, Sypeck described EdgeFuse as "a collection and ecosystem of various tools," of which the smartphone app is only the first component. The company's long-term goal, according to promotional materials, is to "provide defense contractors with a rich library of real problems worth solving."
To date, Mav6 is best known for their work on a gigantic missile-equipped spy blimp, the Blue Devil II, that David Axe of Wired's Danger Room blog detailed got caught in a convoluted tug-of-war with the Air Force and other defense contractors. Mav6 is currently working on deals with other branches of the American military, as well as international organizations like NATO. The University of Colorado Boulder's Boulder Digital Works (BDW) is partnered with Mav6 on the EdgeFuse project, along with digital strategy firm Undercurrent and designers Future Partners.
In basic terms, Mav6 is trying to leverage social networking to create a collaboration platform for engineers, researchers, and sales teams at defense contractors that also lets military users comment on functionality. Longer-term plans for the project include integration with LinkedIn and Facebook's APIs. EdgeFuse, which will launch in beta in September 2012, will also reward users with "points" for sharing information. By creating a secure social network for defense contractors, the idea is that visual sharing for the field as a whole will drive R&D costs downwards.
According to BDW's David Slayden, EdgeFuse allows Mav6 to "hack the defense acquisition bureaucracy" by connecting military end-users with ideas generated outside of the military by start-ups, small businesses, innovators, and entrepreneurs. Future apps developed by graduate students in the BDW program for Mav6 include a "What's F**kin' Broken" tool that allows troops to enter problems with the equipment they're using and watch the progress of their complaint ticket.
For Mav6, the primary motivation behind EdgeFuse appears to be the unoccupied niche for collaboration platforms in the defense industry. Due to very specific security requirements and issues related to everything from the use of personal smartphones for work to preserving trade secrets, collaborative platforms for defense contractors have lagged behind those in most other scientific sectors. Defense contractors have money to throw around; a smartphone-based collaborative tool that meets Bring Your Own Device (BYOD) scrutiny could be very lucrative indeed.
Most importantly, targeting defense contractors and the third-party firms that do business with them is good business for Mav6 and other small- and medium-sized defense contractors. The United States' defense budget has entered a new phase of belt tightening, with savings goals made for most military branches that almost guarantee a clampdown on super-lucrative contracts. Selling the defense industry on social networks and collaborative tools that have been around the private sector for a while simply makes good business sense.
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@ Brendon. Yep. War is bad news for longevity of life. But it's unfortunately a part of our world today. EdgeFuse is a way to design faster solutions -- and get those solutions into the hands of edgefighters faster -- to prevent the deaths of those fighting to overcome hateful regimes and/or establish stability within each respective theatre.
I can think of 50-60 other places in the world that could desperately use a "Whats F***ING Broken?" app.
50-60 at least. And why is Co-Exist---meant to highlight stories that "alter the way we live for the better" ---writing on military innovation? Are not war and death the exact opposite of existence...?


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this is standing on top of a hill on the 7 Gates Road heading into Lake Eucumbene, with the Snowy Mountains as a background
Laguna Blanca, La Cumbre Country Club, and the Santa Ynez Range near Santa Barbara, California.
I went on a bit of a walkabout outside of Hursley, and discovered why the English talk of 'walking holidays' and why they value walking through the countryside. Short answer: paths and trails and beautiful country.
- Sold Out Darius Rucker True Believers Tour, Enid Event Center and Convention Hall, Enid, Oklahoma with Eli Young Band and David Nail, March 7, 2014 by Mike Klemme
Centro de Tequisquiapan, en el Estado de Querétaro, Qro; Mexico Plaza Miguel Hidalgo, al fondo el Templo de Santa María de la Asunción This is the center of Tequisquiapan in Queretaro, ; Mexico Square Market, Miguel Hidalgo, and the Santa Maria de la Asuncion Temple. www.wix.com/elpavi/elizabeth ...
This 360 panoramic shows a wedding reception setup, just a few hours before beginning, at the LittleEasy on the Green at the Detroit Country Club. See www.SpeakEasyDL.com for more information about reserving our Banquet or Catering Service.
Caserio en Iturrioz, cerca de Ernio. Tipical Basque house in village Iturrioz near San Sebastian, Guipuzcoa, Basque Country (SPAIN) House on the left is actually a small church - chappell.
Another shot of the Badlands this time as the sun was receding. After turning off I-90 onto 240 near the town of Cactus Flat we entered the Northeast Entrance of Badlands National Park. I took my first Gigapan at the Big Badlands Overlook and then moved south through the park toward the town of Interior stopping befo...


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Top Baby Products! From strollers to rockers and more, these are the top baby products that parents - and gift givers - will want to know about!
Kaia Compact Stroller
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from Manhattan Toy $22.09
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from VTech $12.99
Go! Go! Smart Friends Trot & Go Pony
from VTech $14.99
Peanuts Ryan Baby Bag
from LeSportsac $168.00


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Author Issa Asad
Issa Asad's latest book Issa Asad Instant Profits with Instagram book has been a huge hit on Amazon for its no-nonsense look at how to use Instagram for your business. Instagram is the fastest growing social media site. It has millions of users and is expanding rapidly, but most businesses haven't figure out how to harness the power of Instagram.
Li-Young Lee's fifth book of poetry, Behind My Eyes, is a tender hymn to the hardships of his childhood, expertly combining the feelings of an adult who's seen too much of the world with the innocence of a child who doesn't understand the world's injustice.
In addition to holding a third-degree black belt, Francis Levy knows a thing or two about raising hell. Still, after glutting his first two novels with a blitz of psychosexual disorders and more bare-assed buggery than a stroke mag, should a stranger stop Levy on the subway and gush about being a huge, HUGE fan, the author's first instinct would have to be an uh-oh backward step.
As someone who rabidly composes and reads mixed-genre works it is often the case that I can only open a book of "prose" or "stories" and read a small portion of it before I begin to get distracted or frustrated. I admit that I am a stimulus junkie and the usual pace of more traditional prose or story-based works is hard for me to stay in.
In Act I of Death of a Salesman, Willy Loman is growing increasingly upset at his lack of accomplishment. At sixty years old, he is working on commission, he can't manage to pay his bills without borrowing money from his neighbor, and his son Biff is working odd jobs on ranches out West, directionless. Willy bemoans the current state of life to his wife, Linda. He has been working his entire life and it has all added up to nothing. Even when they finally pay off their house, there will be nobody to live in it, to which Linda replies, "Well, dear, life is a casting off.
To ask an American poet about international verse is often a waiting game, wherein one counts how many ticks of the second hand it takes for the responder to sing Neruda's praises, quizzically gaze out the window, and deftly change the subject after a dramatic pause like a Wes Anderson protagonist. With the proliferation of, and plurality within, our many aesthetic cliques, it is lamentable that so few of us (this reviewer included) break out and explore the many emerging voices in the grand chorus of English language poetry.
I began reading Ethel Rohan's Hard to Say(PANK 2011), a slim volume of very short fiction, with very little exposure to her previous work. I'm not sure what I was expecting from those fifteen little stories, but it's fair to say that I was unprepared for just how deeply I would be affected by them.
When I first received Sarah Rose Etter's Tongue Party in the mail, I knew nothing about it other than it had won the 2010 Caketrain chapbook competition. In hindsight, I'm extremely glad I knew nothing about this collection, because watching each beautiful, terrifying, utterly bizarre story unfold is part of what makes reading this cohesive collection so enjoyable.


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Not surprisingly, there's some massive interest in Sarah Palin's nature-themed reality television show about Alaska. Discovery Communications and A&E Networks are apparently the top bidders for the program — which would focus on the ex-governor giving a guided tour of her native Alaska.
Obviously, this one is all about the potential ratings — since linking Sarah Palin to environmental conservation is laugh-out loud tenuous. Maybe the show would work better as a comedy, inserting a laugh track under every quip about how much she loves nature and all its inhabitants.
Speaking of hilarity, Palin reportedly wants between $1-$1.5 million per episode — a huge amount for a freshman cable series.
"It will sell," predicted one insider to Reuters. "One way or another." And that's the sad truth.
About Michael dEstriesMichael has been blogging since 2005 on issues such as sustainability, renewable energy, philanthropy, and healthy living. He regularly contributes to a slew of publications, as well as consulting with companies looking to make an impact using the web and social media. He lives in Ithaca, NY with his family on an apple farm.
View all posts by Michael dEstries →
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I'm looking to prototype some paintball markers (guns) I've been creating in 3D. I've never made a gun in my life so this is extremely difficult to model because I don't understand the mechanisms in a certesian sense. But I'm working on it. I'm not even sure if these guys will do guns or not. Anyway, hi! I'm a teenager, but don't let that turn you off to my experience and skill.
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Tag:New York Jets
Posted on: August 15, 2010 8:43 pm
LT not thrilled by Gates and Rivers' comments
Posted by Will Brinson
A few weeks ago, we discussed a feature out of San Diego in which Philip Rivers and Antonio Gates addressed the departure of LaDainian Tomlinson.
Neither Gates nor Rivers bashed LdT by any means, but their comments, which indicated that Tomlinson was the rough equivalent of a dark cloud floating around the locker room, weren't exactly filled with effusive praise.
In an article from Sam Farmer of the Los Angeles Times from Saturday, Tomlinson responded to their quotes.
"I thought they were my guys," Tomlinson said. "People always say, and my family has said it to me, that you know who your real friends are when you're at your lowest point and you don't have a job or whatever. And guys, they said what they felt, whether they were taking shots at me or really just saying what they felt needed to be said."
Tomlinson also addressed his leaving the Chargers, stating that he "started to see my departure out of San Diego way before" the media did ... although it was apparently only two years ago. (Which, you know, is when we ALL knew it.)
"Obviously, they had to start to build that team around Philip and get the guys they needed around him," he said. "I didn't fit that. That's why I kind of found myself on the outside looking in, and looking for work after this past season."
It's understandable that he'd be upset by the comments from Rivers and Gates -- even if it's their team (and it may have been before even this year), what they said is tough to hear regardless of how far removed Tomlinson is from San Diego.
That being said, though, Gates and Rivers were both honest and accurate and it's tough to fault them just for being candid. Just as it's tough to fault the Chargers for making a smart business decision and letting LT go, even if it was a year too late.
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Category: NFL
Posted on: August 15, 2010 11:46 am
Hot Routes 8.15.10: Overhyping the preseason
Got a link for Hot Routes? Tweet us or shoot an email to andy [dot] benoit [at] cbs [dot] com.
Posted on: August 15, 2010 11:39 am
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Posted on: August 13, 2010 11:00 am
Jets' DBs doing pre-practice rain dance for Revis
Posted by Will Brinson
There's no question that the Jets defense will miss (or does miss, as it were) Darrelle Revis. Even with Rex Ryan's schemes helping to mask certain flaws, no one wants Antonio Cromartie playing the role of No. 1 cornerback on a team that already won the 2010 Super Bowl*.
Which may explain why the members of the secondary are doing a rain dance (for lack of a better phrase) around Revis' jersey on the practice field.
"We just have to keep his memory alive while he's not here," safety Jim Leonhard said of the dance. "The DBs always dance before games. We wanted to let him know that we're still thinking about him and he's still one of us."
Cromartie didn't even think the dance was enough from his end, either, running over to the right side of the field right before the snap as a tribute to the missing DB.
"That's his side," Cromartie said of why he vacated the left side. "I went over to the right side for that one play. That was my tribute."
Clearly, they like him. And miss him -- although it's unlikely that any sort of dance, short of one that can produce $15.2 million a year, will bring Revis back. His relationship with his teammates isn't the issue -- it's the relationship with the team that's the problem.
*only in Rex Ryan's mind
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Category: NFL
Posted on: August 12, 2010 9:25 pm
Namath has a take on Revis v. Jets
Of course he does, baby! It's Broadway Joe (dot, um, t-v)! Anyway, Joe's got his take up on his new fancy website about Darrelle Revis and the Jets and it's worth watching, if only for the stunning homemade v-neck 1982 Nike cutoff t-shirt that he's wearing.
I can't tell for certain, but I feel confident he may be wearing jean shorts. Bonus: at the three minute mark as he starts discussing whether or not "life is fair," his dog starts barking. Still, it's interesting nonetheless, particularly when Joe draws on his own experience to point out that if Revis goes back and plays hard for the Jets and eventually gets paid there, New Yorkers will continue to love him.
Category: NFL
Tags: Darrelle Revis, Joe Namath, New York Jets
Posted on: August 12, 2010 12:00 am
Hard Knocks, episode 1 review
Posted by Josh Katzowitz
I talked about this in my preview , but I didn't love last year's version of Hard Knocks, featuring the Bengals. So, I was interested tonight to see if I could resume my love affair with the show if I wasn't intimately involved with covering the team. And the end result: Yeah, it was pretty good. Had to set up some storylines, but there were some laughs and some good in-practice footage. I'll keep watching.
-The first voice we hear is coach Rex Ryan, and he's already very salty. While talking to his team before he releases the players for the summer, he says, "We're right where we want to be, no question about it. Enjoy this time. Get away from it. No matter what you're doing, it's better than my (bleeping) vacation. I'm cruising the Baltic's with my in-laws and (bleep)." Great, great stuff already from Rex.
-I probably shoulld have counted his F-bombs, but I never did. If I had to guess? Maybe 30.
-It's not often you get to see players training in the offseason. Dragging a tire behind you as you run hills doesn't look fun.
-Like usual: a great intro. I love watching the mundane stuff you see every day but would never stop to focus on. Stuff like the FieldTurf spraying when a guy runs his sneaker along it in slow motion. I remember one day before practice last year when a Hard Knocks camera crew spent 30 minutes filming a worker painting one of the lines on the field before Bengals practice in Georgetown, Ky. Of course, the next week on TV, it looked like the most interesting thing in the world. Sure enough, just after I wrote that, the crew filmed somebody spray-painting a line in Cortland.
-Somehow I expected Rex Ryan to look thinner. But yeah, he looks better than before. Just not as much as I thought after his surgery.
-I was wondering how much they would focus on Darrelle Revis. I was wondering if they'd have filmed him. The answers: a pretty fair amount and no.
-Rex knows how to draw laughs from his squad. "Revis isn't in this building right now. Does it matter that Revis isn't here? Goddamn, he's pretty (bleeping) good." Big laugh.
-Ah, a Laveranues Coles sighting. This is his second Hard Knocks in a row. He must be loving it. That's a joke, of course. Coles does not like dealing media. And when I say does not like, I mean he hates it. I interviewed him just once last year – which was more than just about anybody else - and he got pissed at me and abruptly ended it after a couple questions.
-How about Buddy Ryan in a tie on the sidelines in that archived film? I think Rex should bring back that look.
-Yep, we've got our long-shots to make the squad. Brashton Satele, a rookie LB, and Aaron Kia, a rookie OL from Hawaii. OL coach Bill Callahan is talking about out how Kia played in a spread offense in college and how he doesn't know much the techniques around the NFL. Not surprisingly, he's getting coached up quite a bit. Rex Ryan then calls him awful. Ugh. Satele, meanwhile, is impressing some people. Then, he sprains his ankle – which drastically decreases his chances of making the team.
-Lots of people being fined. Apparently, it costs you $5 if you're caught running shirtless. Even Rex isn't immune. He's fined $1 per snacking offense.
-How about the hair-pulling by GM Mark Tannenbaum when dealing with the Revis stuff> I thought he was going to pull it out of his head.
-It's a half-hour into the show before we're introduced to Mark Sanchez. And he's telling LB Bart Scott to shut up.
-Good lord, Joe Namath looks like my grandfather getting out of the front seat of that SUV. High belt line and a bit stooped. He does make a good point about how people shouldn't fumble.
-I love the skills competition between Rex and Tannenbaum.
-Could there be a better name for a bone-crushing fullback than John Conner, who's obviously nicknamed the Terminator? He should make the team just on his name alone.
-Well, they didn't paint the Revis picture in very positive terms after the diner meeting, did they?
-We have our first casualties. Rookie LB Kevin Basped and his bad knees are let go, and Kia is laid off as well. Poor dudes. They don't even make the second episode. Kia is told, though, that he could play in the UFL. So, he's got that going for him. But man, he looks pretty pissed. Kia hands in his playbook, slams the door in the cameraman's face and the episode ends.
-Once again, solid work from HBO. Looking forward to next week
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Category: NFL
Posted on: August 11, 2010 6:33 pm
Edited on: August 11, 2010 7:12 pm
Top moments of Hard Knocks
Posted by Josh Katzowitz
Hard Knocks premieres tonight at 10 p.m. ET on HBO, and it's tough not to be a little excited. I've watched every season of the show, even when I was a casual NFL fan. Even when while watching teams I didn't care much about, the drama, the film-making, the voice-overs and the interviews make for a beautiful viewing experience.
Last year, while covering the Bengals for CBSSports.com, I was a part of it. Early in training camp – actually, I believe it was in Episode 1 – TE Ben Utecht went down with a scary-looking neck injury, and it's likely the last time Utecht will step on a field. After the ambulance took him away, Bengals PR extraordinaire Jack Brennan got all the reporters together on the side of the field to give us an update.
The Hard Knocks cameras were there, and they got each of our faces as we listened hard for the news. The next day, a buddy said to me, "Hey man, you look like crap in HD." And that was the extent of my starring role on Hard Knocks.
I didn't love the show last year, probably because I already knew what had happened by the time each episode aired – one of the best parts of the show is the drama that's built by the fringe guys trying to make the 53-man roster – and because I learned a few of the tricks that go into making a TV show like that.
But aside from that, it's just a cool behind-the-scenes experience (and frankly, most everything that HBO shoots is gorgeous), and this year, it should be extra fun because the ever-loquacious Rex Ryan will be one of the stars. I'll watch the show tonight and get my review of it up on the blog about midnight or so. Until then, here are the top three moments (as picked by me) in Hard Knocks history.
3. Dallas' Marion Barber playing the piano (I'm a huge fan of somebody who's not a pianist that can actually play the piano. That's one reason I love Chico and Harpo Marx, and why Zach Galifianakis has become such a big star).
2. Kansas City's Bernard Pollard can dance! In his underwear! Like a stripper! With his teammates going wild around him!
1. Marvin Lewis, in a phrase the Bengals beat writers imitated all season long: "Be a pro!" Warning: this video has some naughty language.
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Category: NCAA Hockey
Posted on: August 11, 2010 5:12 pm
Ryan really wants Revis back at practice
Posted by Josh Katzowitz
Rex Ryan sounds serious about solving this mess between his New York Jets club and CB Darrelle Revis. He said today he would cancel practice and get the entire team together with Revis and his representation to hammer out a solution to the contract dispute and end his hold-out.
Seriously, Rex? Oh yeah. He said he was absolutely serious.
"This is what I would like to have happen -- for everybody to put their cards on the table," Ryan told reporters, including the NY Daily News . "Have Darrelle come here with anybody he wanted. We'll have (owner Woody) Johnson here. In fact, this is the way I would handle it: We'll call off practice. We'll have our whole team there and meet. That way there's no he said-she said. Just get the thing done. And let's work it that way.
"Let's do this. I'm inviting them to come in - Darrelle to come in with everybody else. Anybody he wants. We'll have the whole team there, including the owner. The entire organization. … And then that's maybe how we'll get a solution. Everybody wants a solution. Well, let's figure a way to do it. Maybe this is a way."
OK, I've covered a few fun coaches/managers on the beat in the past – my favorites were Brian Kelly and Dusty Baker, just for the way they could fill up a notebook with fantastic anecdotes – but man, how great would it be to have to talk to Ryan every day?
Word out of Jets camp has been that New York's defense could withstand the loss of Revis – in fact, Ryan said that very thing the other day – with Antonio Cromartie and rookie Kyle Wilson in the secondary. But obviously, they need Revis to compete for a Super Bowl. And they know they need Revis to compete for a Super Bowl.
Manish Mehta caught up with Neil Schwartz, Revis' agent, after Ryan's proclamation. Said Schwartz: "If the Jets want to have a meeting, the Jets know how to reach us."
I said Tuesday on CBS's Around the League video that I don't believe Revis will miss a regular-season game for the Jets. After Ryan's idea today, I'm not changing my mind.
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Category: NFL


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Firefighters found the Johnson family's boxer Sascha hiding in the basement.
- Filed Under
An Indianapolis family -- and their dog -- are safe after a fire ravaged their Far-Eastside home this morning.
Fire crews arrived at the residence, 1940 Herford Drive, at 10:12 a.m. expecting a routine residential alarm. But when they arrived, they saw fire and smoke pouring out of the two-story house.
Crystal Johnson, 52, and her 10-year-old daughter weren't home ...