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Dear Internet: Chill the @#*! out.
Yoga and health guru Rainbeau Mars, who has sold over 1 million yoga DVDs and worked with a long list of celebs, is being labeled a bridezilla after asking guests to her upcoming nuptials to participate in a three-week vegan cleanse prior to the ceremony.
"In celebration of Rainbeau and Michael's upcoming Hawaiian wedding, join our global community to become the most beautiful, healthy and fit versions of ourselves!" the invite offers.
The "Three Week Superstar Glow Cleanse" Rainbeau offers on her website is a "zero calorie restriction, plant-based food revitalization program" that claims to claims to strengthen the immune system, prevent disease, and release stress.
Sadly, people misconstrued Rainbeau's request and began labeling it as an unfair "demand" on her guests; as if the health instructor was going to personally make sure everyone went vegan for three weeks. No seriously – people believed this.
Writing on HuffPo, Rainbeau said:
"I invited the people who are attending our ceremony to join us as we also prepare for our sacred vows. This wedding is about all of us with intentions to purify in the garden. The invitation was not a demand or a requirement and I am sorry if any of you were offended because of this thought. However… your giving me a great idea… what if I really had made it a requirement…that will have to be the next one. JOKING! Hope you'll all give our fall cleanse a try. I think we could all use a little more love and support around here. From my heart in gratitude. Rainbeau Harmony Mars and yes thats my real name."
Oh by the way – have you heard where the wedding is located? Three days on the island of Kauai in Hawaii. Guests will enjoy unique activities from breath work classes to yoga to horseback riding. Who wouldn't try a plant-based cleanse to enjoy something like that?!
Check out Rainbeau's 3 Week Pre-Wedding Cleanse invite video below.
About Michael dEstriesMichael has been blogging since 2005 on issues such as sustainability, renewable energy, philanthropy, and healthy living. He regularly contributes to a slew of publications, as well as consulting with companies looking to make an impact using the web and social media. He lives in Ithaca, NY with his family on an apple farm.
View all posts by Michael dEstries →


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COP19: Activists protest 'climate criminals' attendance at talks
Samantha Smith , WWF
From Warsaw: COP19 UN Climate Change Conference 2013
COP19 (16/11/13) – Protesters took to the streets of Warsaw to register their anger at a lack of progress at the climate talks.
One of the marchers says representatives from the fossil fuel and transport industries have wrapped themselves in the colours of the UN.
Joining the protesters, Samantha Smith, global climate and energy initiative leader at WWF, says governments may have come to take part in climate change negotiations, but it seems we're moving backwards because the host government is holding conferences with the coal industry, Japan has walked away from emissions reduction commitments and Australia has made it unclear on what its plans for the future are.

subset: commoncrawl


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Essential Strategies
RP sets consist of two elements: passages and items. Therefore, we must learn how to handle the passages and the items separately. We'll use a long nonfiction RP to introduce the key methods you'll want to use for tackling each element. Then we'll turn to short RPs, paired RPs, and fiction RPs to show you how to modify the methods to handle these "special cases."
Generally, you'll want to deal with the passage before you look at the items. As you read through the next sections on how to tackle the passage, keep in mind the major types of items you'll be asked to answer:
- Recognizing the major features of an RP: topic, main idea, purpose, tone, theme, and logic.
- Recognizing the use of rhetorical devices and literary techniques, including: hyperbole, repetition, imagery and figurative language, sound patterns, rhetorical questions, idioms and clichés, irony, foreshadowing, and motif.
- Decoding unfamiliar words from context.
- Finding informational details (facts) in the passage.
- Identifying cause and effect and follow the logic of arguments.
- Comparing and contrasting arguments.


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PMC + First letter of First Name + your Last Name
Our new learning center is here to enhance the skills you have and
to ease the task of completing the educational courses required by
your respective licensing boards. We know the strength of our
organization lies within our foundation – the foundation of dedicated
and loyal employees committed to our mission and the patients we
care for. Physicians, discharge planners and other referral sources
entrust their patients to us because of our compassion, comprehen-
sive approach and dedication to assisting each patient return to their
maximum level of independence. And, with the unlimited number of
courses you can take, even beyond your requirements, we are all one
step closer to becoming the best in home health care.
Our goal is to remain on the cutting-edge and provide our employees
the most appropriate and applicable education for today's skilled care
environment. We trust that each of you will take full advantage of this
great benefit and walk proudly each day as a representative of your
profession as well as a part of the PharMedCorp organization.
Please contact your facility manager for information
about Eligibility and Logging in.


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Orange County
Your bridesmaids will thank you for this shopping experience, which is more like a pre-wedding party. Our fashion specialists offer so many different complementing style lengths, colors, and fabrics, you will find the perfect dress to flatter every figure. And don't forget to throw an Album Party to celebrate when you get the gorgeous pictures back!
Plum Pretty Sugar
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Treece - Rogers
Thursday, May 26, 2011 2:56 pm
Tim and Regina Treece of Adamsville, TN are pleased to announce the forthcoming marriage of their daughter Jennifer Lea Treece to Jody Braxton Rogers of Greenhill, AL. The couple will exchange vows in an outdoor ceremony on Saturday, June 11, 2011 at 6:30 p.m. at the home of Mr. & Mrs. Kevin Thomas, 902 North Maple Street, Adamsville, TN. A poolside reception will immediately follow. All family and friends are invited to attend.
The bride-elect is the granddaughter of the late Ned and Martha Buford of Memphis, TN and Wanda Treece and the late Jimmy Treece of Adamsville, TN. Jennifer is a 2003 graduate of Adamsville High School. She is a 2007 magna cum laude honors graduate of Middle Tennessee State University with a Bachelor of Science in Exercise Science with a Minor in Nutrition. She is a 2009 graduate of Milligan College with a Master of Science in Occupational Therapy. She is currently employed as an Occupational Therapist at Hardin Medical Center in Savannah, TN.
The prospective groom is the son of Allen and Charlotte Rogers and the late Cathy Rogers of Florence, AL. He is the grandson of the late Paul and Wava Hurst of St. Joseph, TN and the late Richard and Hazel Rogers of Greenhill, AL. Jody is a 2000 graduate of Rogers High School in Greenhill, AL. He attended Northwest Shoals Community College where he played baseball. He is a 2004 graduate of Wallace State Community College in Hanceville, AL with an Associate in Applied Science degree in Physical Therapy. He is employed as a Physical Therapy Assistant with Amedysis Home Health in Florence, AL.
The couple will make their home in Savannah, TN following their honeymoon.


text:
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text:
Reed Mahoney
- Filed Under
As I began to look ahead to this new year, with an equal measure of hope and cynicism, I found myself, at the same time, looking back. In spite of advancing age, it appears I am still capable of learning, and 2013 brought me many opportunities to expand my knowledge of the world and its nature. It's a good exercise, I think, so with your permission I'll share some of that which I've gleaned over the last 12 months.
I learned, for example, that in spite of what Sarah Palin and her tea party compatriots in the DNC (that's "Do Nothing Congress") say about a law they voted to rescind at least 43 times, Death ...


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2013 Survey Results
We do believe we build the best equipment but we are always looking for feedback from our clients so we can continue to improve our products and services.
Based on our never ending quest to improve we conduct an annual survey with each of our locations. We reach out to the owner or manager directly and ask them to respond to the 3 questions below, ranking their answers from 10 being the best, to 1 being the worst.
- How would you rate the speed and quality of our service?
- How would you rate the reliability and durability of your LASERTRON equipment?
- When the time comes to invest in a new system, would you definitely go with LASERTRON? 10 you would, 1 you wouldn't.
Once again our survey for 2013 validates that we are achieving our goal of providing our clients with the products and services they need to succeed.
The average score for question 1 on the speed and quality of our service was 9.0.
The average score for question 2 regarding the reliability and durability of our system was 8.5. Keep in mind that more than 50% of our customers operate with systems that are 7, 8, 9, 10 years old or older!
The average score for question 3 on whether they would use LASERTRON again was 9.8.
We are very lucky to be able to work with great operators. Many of whom attend our conferences on a regular basis and others that we keep in close contact with by phone or email.
We welcome any existing operator of a competitive system, any new bowling center or skating facility as well as any FEC operator to join our growing team of operators. 2013 has proven to be our best year ever and we look forward to working with even more clients in 2014.
Here are some client testimonials we received while conducting our annual survey:
We maintained a relationship with Lasertron the next couple years and in 2010 a fourth project started to take shape. We were determined that we would not make the mistake again. Although we received a similar offer from our current supplier we had finally chosen to open our first Lasertron arena. Since opening this facility in 2011 we have converted 2 of our other 3 centers into Lasertron with plans to convert the third as well. We could not be happier with our decision. Lasertron offers us outstanding service and an excellent product. The reliability of the equipment is far above the competition. We have created more than just a relationship with the staff at Lasertron; we consider it a friendship.
Lasertron has changed the way we do Laser tag!"
For us as an Lasertron operator it is important to have a trustworthy partner, who knows his business. - Meanwhile for more than 24 years. This is the reason, why we decided to open a new Lasertron cybersports arena in Graz, Austria this winter."
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Rasmussen encephalitis (RE) is a rare complex inflammatory disease, primarily seen in young children, that is characterized by severe partial seizures and brain atrophy. Surgery is currently the only effective treatment option. To identify genes specifically associated with the immunopathology in RE, RNA transcripts of genes involved in inflammation and autoimmunity were measured in brain tissue from RE surgeries and compared with those in surgical specimens of cortical dysplasia (CD), a major cause of intractable pediatric epilepsy.
Quantitative polymerase chain reactions measured the relative expression of 84 genes related to inflammation and autoimmunity in 12 RE specimens and in the reference group of 12 CD surgical specimens. Data were analyzed by consensus clustering using the entire dataset, and by pairwise comparison of gene expression levels between the RE and CD cohorts using the Harrell-Davis distribution-free quantile estimator method.
Consensus clustering identified six RE cases that were clearly distinguished from the CD cases and from other RE cases. Pairwise comparison showed that seven mRNAs encoding interferon-γ, CCL5, CCL22, CCL23, CXCL9, CXCL10, and Fas ligand were higher in the RE specimens compared with the CD specimens, whereas the mRNA encoding hypoxanthine-guanine phosphoribosyltransferase was reduced. Interferon-γ, CXCL5, CXCL9 and CXCL10 mRNA levels negatively correlated with time from seizure onset to surgery (P <0.05), whereas CCL23 and Fas ligand transcript levels positively correlated with the degree of tissue destruction and inflammation, respectively (P <0.05), as determined from magnetic resonance imaging (MRI) T2 and FLAIR images. Accumulation of CD4+ lymphocytes in leptomeninges and perivascular spaces was a prominent feature in RE specimens resected within a year of seizure onset.
Active disease is characterized by a Th1 immune response that appears to involve both CD8+ and CD4+ T cells. Our findings suggest therapeutic intervention targeting specific chemokine/chemokine receptors may be useful in early stage RE.
Keywords:Inflammation; Rasmussen encephalitis; Cortical dysplasia; Epilepsy; Gene expression; T cells; Chemokines
Rasmussen encephalitis (RE) is an inflammatory neurodegenerative disease primarily seen in young children. It is clinically characterized by intense focal and generalized seizures with inflammation almost invariably confined to one cerebral hemisphere [1-3]. Seizure frequency may decrease over time, but patients are left with unilateral hemiparesis and significant cognitive deficits [4,5]. Histopathologic examination of RE brain tissue, usually after years of seizures, reveals the presence of activated microglia and T lymphocytes [6-8]. CD8+ T cells containing granzymes are observed in close apposition to neurons and astrocytes, but not oligodendrocytes [6,9]. Auto-antibodies against the glutamate receptor GluR3, once considered a possible cause of RE [10], are not present in all RE cases and are not specific to the disease [11-13]. Circulating antibodies to other neuronal proteins are found in other RE cases [14-16], leading to the view that a humoral immune response may be a secondary event to T cell immunity [6,17]. Thus, RE resembles an autoimmune disease although the histopathology is also consistent with an immune response to a virus or other infectious agent [1,17]. Cytomegalovirus, herpes simplex virus, and Epstein Barr virus sequences have been detected in some RE brain specimens, but have not been reproducibly identified in all cases [18-22]. Neither autoimmunity nor infection can easily explain the unilateral hemispheric involvement.
To gain further insight into the immunopathology of RE, we measured the relative expression of 84 mRNA transcripts associated with inflammation and autoimmunity in brain tissue from 12 RE and 12 cortical dysplasia (CD) epilepsy patients. The CD cases constituted a reference group against which to compare gene expression levels. Inflammation is associated with CD, but is less severe than in RE, and T cell involvement is limited [23,24]. Quantitative differences in transcript levels between RE and CD specimens were found for several genes involved in the activation and recruitment of effector T cells. The highest levels of expression were found in early stage RE cases.
Cohort recruitment and clinical variables
Under University of California, Los Angeles, (UCLA) Institutional Review Board (IRB) approval, brain tissue was collected at surgery as part of UCLA's Pediatric Epilepsy Surgery program. Informed consent to use the surgically resected tissue for research was obtained through the parents or legal guardians. The 12 RE and 12 CD cases used in the study were selected with similar ages at surgery. Seven CD cases were classified as CDI, and five as CDII [25]. The clinical protocols for patient evaluation and surgical procedures for collection and processing of cortical specimens have been previously published [26,27]. Clinical variables included, age at seizure onset, age at surgery, and disease progression (age at seizure onset to age at surgery). Magnetic resonance imaging (MRI) scans were assessed by one investigator (GWM). A semiquantitative score was assigned to the T2 and FLAIR signal changes (0 = none, 1 = slight; 2 = mild; 3 = moderate; 4 = extensive) to estimate the degree of tissue destruction and inflammation, respectively.
Real-time PCR and analysis
Total RNA was purified from flash frozen blocks of involved tissue consisting of mostly cortical gray matter (approximately 50 mg) using Trizol™ (Life Technologies, Carlsbad, CA, USA) and reverse transcribed (Qiagen, Valencia, CA, USA). PCR reactions were carried out in an ABI 7300 thermocycler using SYBR™ green chemistry (SABiosciences, Valencia, CA, USA). The 96-well format qPCR array contained primers for 84 genes of interest and 5 reference genes (SABiosciences inflammation and autoimmunity qPCR array, cat no. PAHS-0077Z). Standard cycling parameters were used as follows: 1 cycle: 95°C 10 min, 40 cycles: 95°C 15 sec, 60°C 1 min. As part of the cycling program, all PCR products were thermally denatured and dissociation curves were obtained. Two of the primer sets resulted in dissociation curves with multiple peaks (CCL16 and CCL24) and were eliminated from the analysis. To calculate relative transcript levels, baseline-subtracted fluorescence values per cycle for each primer set were entered into LinRegPCR [28] and PCR efficiencies (E) and Ct values were determined. The relative expression of each gene (X0) [29] in each array was calculated from:
NormalFinder [30] was used to identify the least variable housekeeping gene across the 24 arrays. ACTB encoding β-actin was found to be the most stable reference gene, and was used to normalize all of the data including the four other reference genes (HPRT1, RPL13A, GAPDH, B2M). Samples were grouped by consensus clustering [31] using the non-negative matrix factorization algorithm [32] found in the GENE-E bioinformatics package (http://www.broadinstitute.org webcite). Statistical analyses utilized R-project programs (http://www.r-project.org webcite). Plots were generated by the Deducer graphical user interface and exported into CorelDRAW X6 (Corel Corporation, Ottawa, ON, Canada). Significant differences (P <0.05) in gene expression between RE and CD cases were determined by pairwise comparison of the distribution of transcript levels for each gene using Harrell-Davis quantile estimators [33,34].
Immunocytochemistry and image analysis
Paraffin-embedded blocks of involved tissue were serially sectioned (5 μm), deparaffinized, and microwaved for 20 minutes in buffered citrate (10 mM, pH 6.0) for antigen retrieval. After one hour in blocking solution (Impress Kit, Vector Laboratories, Burlingame, CA, USA) sections were incubated overnight at 4°C with rabbit anti-human CD4 (1:250, Novus Biologicals, Littleton, CO, USA) or mouse anti-human CD8 (1:100, Dako, Carpinteria, CA, USA). Sections were immunostained for one hour at 25°C with peroxidase-conjugated anti-rabbit or anti-mouse secondary antibodies (Impress Kit, Vector Laboratories) followed by incubation with 3,3′-diaminobenzidine (DAB) substrate (MP Biomedicals, Santa Ana, CA, USA), then counterstained with hematoxylin. Sections of tonsil tissue were used as positive controls, and omission of primary antibodies served as negative controls. Images of entire sections were acquired with an Aperio ScanScope XT scanner (Aperio, Vista, CA, USA) and transferred to CorelDRAWX6. Strong DAB staining of CD4 and CD8 immunoreactive cells was quantified using the positive pixel count algorithm, part of the Aperio ImageScope software package.
Western blotting
Blocks of flash frozen involved tissue were homogenized in RIPA buffer containing protease and phosphatase inhibitors (Sigma-Aldrich, St. Louis, MO, USA). Lysates were separated on precast 10% polyacrylamide gels (Biorad, Hercules, CA, USA) and transferred to PVDF membranes (Biorad). Prestained molecular standards were used (Biorad). The membrane was blocked in Tris-buffered saline (pH 7.4) containing 5% nonfat dried milk and 0.1% Tween™ 20, and probed with a monoclonal antibody to hypoxanthine-guanine phosphoribosyltransferase (anti-HPRT 1:1000, Proteintech Group Inc., Chicago, IL, USA). Proteins were visualized with a secondary antibody conjugated to horseradish peroxidase (1:2500, Jackson ImmunoResearch Laboratories, West Grove, PA, USA) using a chemiluminescent substrate (Thermo-Scientific, Waltham, MA, USA). The blot was stripped and re-probed with a monoclonal antibody to glyceraldehyde 3-phosphate dehydrogenase (anti-GAPDH 1:1000, Stressgen, Victoria, BC, Canada). X-ray films were scanned and processed (background subtraction and enhancement using default settings) in Image J, and exported to CorelDRAWX6.
Rasmussen encephalitis and cortical dysplasia patient cohorts
Table 1 summarizes clinical variables for the RE (n = 12) and CD (n = 12) cohorts selected for study. Patient data associated with each specimen are provided in Table 2. The age range at surgery was similar, whereas seizure onset occurred at an earlier age in the CD compared with the RE cohort, thus time from seizure onset to surgery also differed. Other clinical parameters were not significantly different, except for the extent of tissue destruction and inflammation measured by changes in the T2-weighted and FLAIR MRI scans, respectively. These parameters were scored semiquantitatively and reflect more injury/inflammation in RE compared with CD cases (Tables 1 and 2). Figure 1 shows examples of graded MRI scans from an RE (A and B) and CD patient (C and D).
Figure 1. Examples of magnetic resonance imaging (MRI) scans from patients with Rasmussen encephalitis (RE) or cortical dysplasia (CD). Scans were semiquantitatively assessed for T2 (A and C) and FLAIR (B and D) signal changes. The RE case (A and B) was a 13 year old with a five-year history of seizures originating from the right hemisphere. The T2 image was scored severe (4) for tissue destruction (A), and moderate (3) for amount of FLAIR changes (B). The patient with CD (C and D) had abnormality localized to the left parietal area (arrows). The MRI scans were scored zero (no increased signal changes) for T2 (C) and FLAIR changes (D) despite the abnormal anatomy.
Table 1. Summary of clinical variables for Rasmussen encephalitis (RE) and cortical dysplasia (CD) cohorts
Table 2. Clinical variables associated with Rasmussen encephalitis and cortical dysplasia patient specimens
A subset of inflammatory genes distinguishes Rasmussen encephalitis from cortical dysplasia
We obtained normalized expression data for 86 genes (82 inflammatory and 4 housekeeping genes). Transcripts of 71 genes were detected in every specimen, confirming that inflammatory genes are expressed in both RE and CD [24]. Thirteen of the remaining genes were expressed in at least six RE specimens, and were retained for analysis. IL23R and IL1F10 transcripts were detected in only three of the RE specimens and were eliminated, leaving a total of 84 genes. IL23R transcripts were expressed in four of the CD cases, and IL1F10 expression was not detected in any of the CD cases.
We employed a consensus clustering method to organize the blinded samples into two groups. Half of the RE cases (RE1, 2, 5, 6, 10, 12) segregated from the 12 CD specimens, two (RE3, 9) partially segregated, and the remaining four (RE4, 7, 8, 11) could not be distinguished from the CD cases (Figure 2). Thus, 6 of 12 RE cases could be clearly identified based solely on the array data. Sample heterogeneity may partly explain why not all of the RE cases clustered. On the other hand, the six clustered RE cases may indicate differences related to clinical variables such as disease progression [35].
Figure 2. Consensus clustering applied to qPCR array expression data from Rasmussen encephalitis (RE) and cortical dysplasia (CD) specimens. Non-negative matrix factorization was used to determine whether the mRNA expression data could distinguish between RE and CD. With k = 2 clusters, six of the RE cohort were clearly predicted. Two RE cases partially segregated, and the remaining four could not be differentiated from the CD cohort. Heat map is scaled from high (red) to low (white) correlation between samples.
Although the clustering analysis revealed variability within the RE cohort, we proceeded to examine whether the expression of particular genes differed between the RE and CD cohorts. The distribution of transcript levels of 40 genes in the RE set and 36 genes in the CD set did not satisfy the Shapiro-Wilk normality test (P <0.05); >85% of these genes showed P values of <0.01. Therefore pairwise comparisons were carried out using the Harrell-Davis distribution-free quantile estimator method. This generates robust analyses that are well-protected from violations of normality. The analysis uses an adjusted critical value for determining significance that controls for multiple comparisons. Distributions were divided into deciles. Transcript levels of eight genes were significantly different between the RE and CD samples across all quantiles (P <0.05; [see Additional file 1: Table S1]). Seven genes were expressed at higher levels in the RE samples; whereas hypoxanthine-guanine phosphoribosyltransferase (HPRT) mRNA levels were reduced (Figure 3). Western blot analysis showed less HPRT protein in tissue lysates that were available from two of the RE specimens compared with one of the CD specimens (Figure 4). The other housekeeping genes did not differ between the RE and CD groups [see Additional file 1: Table S1]).
Figure 3. Expression levels of genes that were significantly different between Rasmussen encephalitis (RE) and cortical dysplasia specimens. Stacked dot plots of normalized expression values for each sample are shown. Solid circles identify the six RE specimens separable by consensus clustering (Figure 2); open circles identify the remaining RE cases. The RE sample with the highest inflammatory gene transcript level is indicated.
Figure 4. Hypoxanthine-guanine phosphoribosyltransferase (HPRT) protein levels differ between Rasmussen encephalitis (RE) and cortical dysplasia (CD) specimens. Western blot showing lower amounts of HPRT in two RE samples (RE1 and RE2) compared with a reference CD specimen (CD5). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) was used as a loading control.
Additional file 1: Table S1. The Harrell–Davis distribution-free quantile estimator method applied to a pairwise comparison of the expression of each gene.
Format: DOCX Size: 30KB Download file
Four of the seven genes with higher expression in the RE specimens, that is, the cytokine IFN-γ, and the chemokines, CCL5/RANTES (regulated and normal T cell expressed and secreted), CXCL9/MIG (monokine induced by IFN-γ), CXCL10/IP-10 (IFN-γ-induced protein 10), play a role in T cell-mediated immunity (see Discussion). Relative expression levels of these genes in the RE cohort were highly positively correlated with each other (Table 3); the lower amounts of these transcripts in the CD cohort were not correlated (Table 4). The remaining genes encode two other chemokines, CCL22/MDC (macrophage derived chemokine) and CCL23/MIP-3 (macrophage inflammatory protein 3), and Fas ligand (FasL). FasL mRNA levels positively correlated with IFN-γ, CCL5, and CXCL9 mRNA levels (Table 3); correlation between FasL and CXCL10 did not quite reach the threshold for significance. The amounts of CCL22 and CCL23 mRNA did not correlate with expression of the other genes (Table 3).
Table 3. Cross-correlation matrix (Spearman rank correlation coefficients) generated by comparing gene expression levels with clinical parameters of the Rasmussen encephalitis cohort
Table 4. Cross-correlation matrix (Spearman rank correlation coefficients) generated by comparing gene expression levels with clinical parameters of the cortical dysplasia cohort
Inflammatory gene expression correlated with clinical parameters
We next asked whether the expression of the seven inflammatory genes associated with RE correlated with clinical variables (Table 1). Time from seizure onset to surgery, a measure of disease progression, negatively correlated with mRNA levels for IFN-γ, CCL5, CXCL9 and CXCL10 (P <0.05; Table 3). By contrast, in the CD group the lower amounts of IFN-γ mRNA positively correlated with time from seizure onset to surgery (P <0.05; Table 4). Positive correlations were found comparing FasL mRNA and severity of inflammation, and CCL23 levels and tissue destruction as assessed by FLAIR and T2 MRIs, respectively (Table 3). As shown in Figure 5A and 5B, the data for IFN-γ and CXCL9 best fit nonlinear functions. Of the six RE cases identified by consensus clustering, five correspond to the earliest times points (filled circles in Figure 5A and 5B), suggesting that an early highly inflammatory phase distinguishes RE from CD. The FasL and CCL23 results are best described by linear functions (Figure 5C and 5D). The number of antiepileptic drugs that the RE and CD patients were receiving at the time of surgery did not significantly correlate with the expression of any of the seven inflammatory genes (Tables 3 and 4).
Figure 5. Correlations between clinical parameters and gene expression. Transcript levels of four of the inflammatory genes that were expressed at a significantly higher level in the Rasmussen encephalitis (RE) compared with cortical dysplasia specimens were plotted against clinical parameters. Negatively correlated parameters are best described by non-linear inverse functions (A and B), whereas linear functions can be fitted to the positively correlated parameters (C and D). Solid circles identify the six RE specimens separable by consensus clustering (Figure 2); open circles identify the remaining RE cases.
T cell infiltration in Rasmussen encephalitis brain correlated with levels of inflammatory genes
Based on our findings we predicted that a more pronounced T cell infiltration would be evident in tissue specimens with higher expression of IFN-γ mRNA. Sections from blocks that were available from seven of the 12 RE specimens were immunostained for CD4 and CD8 expression (four from the group of six with the highest levels of IFN-γ mRNA: RE1, RE2, RE5, and RE10, and three from the remaining specimens: RE3, RE4, and RE8). Quantitation of the strongly immunoreactive cells in digitized images of the stained sections revealed that there were more CD4+ and CD8+ T cells in sections from three out of the four cases with higher IFN-γ and related chemokine mRNA expression (Figure 6; filled circles). CD4+ T cells were associated with clusters of CD8+ T cells, but were mainly concentrated in leptomeninges and perivascular (Virchow-Robin) spaces, which contained fewer CD8+ T cells (Figure 7).
Figure 6. Relative numbers of CD4+ and CD8+ T cells in sections taken from 7 of the 12 Rasmussen encephalitis (RE) cases. Digitized images from serial sections immunostained with anti-CD4 and anti-CD8 were quantified, and indicate higher numbers of both CD4+ and CD8+ T cells in three of four RE cases identified by consensus clustering (filled circles) compared to three RE cases that were not distinguished from cortical dysplasia (open circles).
Figure 7. Photomicrographs showing CD4+ and CD8+ immunoreactivity in Rasmussen encephalitis (RE) specimens. Adjacent sections from specimens expressing high chemokine mRNA levels show a greater concentration of CD4+ T cells than CD8+ T cells within leptomeninges (A and B) and perivascular spaces (C and D), and fewer CD4+ T cells (E) within clusters of CD8+ T cells in brain parenchyma (F). Images are taken from specimens RE2 (A and B), and RE10 (C-F). Scale bars correspond to 50 microns (A and B), and 25 microns (C-F).
We used a qPCR array to measure inflammatory gene expression in 12 RE specimens and 12 CD specimens, and then correlated the expression of genes that differed between the two groups with clinical parameters. Selection of the CD specimens from the UCLA Pediatric Surgery Program's tissue bank was based on choosing patients whose ages at surgery were approximately the same as the much rarer RE cases that took approximately ten years to accrue. There were no other exclusion criteria. The array included genes encoding pro-inflammatory T helper 1 (Th1) and anti-inflammatory T helper 2 (Th2) cell cytokines, chemokines, Toll-like receptors and other factors involved in downstream signaling. The expression levels of only eight transcripts significantly differed between the RE and CD specimens. With the exception of HPRT mRNA, the transcripts were expressed at higher levels in the RE specimens. Reduced HPRT, a housekeeping gene on the array, was unexpected and may reflect disease-associated atrophy. Alternatively, a reduced level of HPRT in brain tissue may be significant since a decrease in this enzyme would be expected to affect purine metabolism, with possible effects on brain function [36]. Analysis of HPRT protein levels in more RE samples will be necessary to establish the significance of this finding to the disease process.
In RE there is clear histopathologic evidence for the involvement of CD8+ T cells in the disease process [6-8,23]. In agreement with these data, four of the seven genes whose mRNA levels were higher in RE compared with CD cases encode proteins involved in Th1-driven immune responses, namely IFN-γ, CCL5, CXCL9 and CXCL10. Activated CD8+ cytotoxic T cells (Tc1) and CD4+ Th1 cells produce IFN- γ [37,38], thus infiltrating CD8+ T cells in neuropil and CD4+ T cells in Virchow-Robin spaces and leptomeninges (Figure 7) could be sources of IFN-γ transcripts in the RE brain specimens. Although the immunopathology in RE appears to be driven by Tc1 cells, CD4+ cells, in the perivascular space may play a role in sustaining Tc1 activity [39-41]. Natural killer (NK) cells, NKT cells, and γδ T cells produce IFN-γ [37,42], which may also account for the IFN-γ transcripts in RE tissue. To date, we and others have found no evidence for significant numbers of NK cells in RE brain tissue [7]. However, we have recently identified γδ T cells in brain infiltrating lymphocytes isolated from fresh RE brain tissue (unpublished data).
IFN-γ can induce major histocompatibility complex (MHC) class I molecules on the surface of neurons, rendering them vulnerable to attack by autoantigen-sensitized MHC class I-restricted Tc1 cells [43]. Further, IFN-γ has been shown to induce bursting of hippocampal pyramidal neurons in vitro[44], providing a possible link between T cells and epileptogenesis.
It has been reported that IFN-γ induces the production of CXCL9 by microglia and CXCL10 by microglia and astrocytes [45], and promotes IL-1-induced synthesis of CCL5 by astrocytes [46]. The positive correlation between the relative amounts of CXCL9, CXCL10, CCL5 and IFN-γ mRNA that we observed among the RE specimens is consistent with these reports. All three chemokines have been implicated in attracting Th1, Tc1, γδ T cells and NK cells to sites of inflammation [47]. The presence of CXCR3, the receptor for CXCL9 and CXCL10 [48], and CCR5 (CCL5 receptor) on infiltrating T cells in brain sections from a single RE case has been documented [49]. Other single patient studies have provided evidence for the expression of CCL5 and CXCL10 in RE brain tissue [50,51].
Quantitative PCR allowed correlations to be made between the amount of inflammatory gene transcripts and clinical variables. Notably, much higher levels of IFN-γ and CXCL9 mRNAs were detected in specimens from patients that had undergone surgery within shorter times from disease onset compared to later times. This suggests that there is a pronounced Th1 immune response in the early phase of the disease that declines after 1 to 2 years. The observation of large numbers of CD4+ and CD8+ lymphocytes in sections from specimens in which high levels of IFN-γ mRNA were detected is consonant with an initial Th1 polarized response. In support of these data, it was previously reported that cerebrospinal fluid levels of IFN-γ were higher in the earlier stages of RE [52].
A role for the Fas/FasL-mediated cell death pathway in RE is indicated from the qPCR data. Based on the observation of granzyme B immunoreactivity in T cells in close apposition to neurons and astrocytes, it has been suggested that MHC class I-restricted killing occurs by the perforin lytic pathway [6,9]. However MHC class I-restricted killing of neurons can also occur by Fas ligand-induced apoptosis [53]. On the other hand, the detection of FasL transcripts in the present study could be explained by activation-induced cell death of activated T cells in the brain [54]. In experimental autoimmune encephalitis, astrocytes expressing FasL have been implicated in T cell homeostasis [55].
In contrast to the chemokines associated with a Th1 response, CCL22, which binds CCR4 [56], is associated with a Th2 polarized response, which can facilitate B cell activation [38]. The finding of higher levels of this chemokine in RE brain tissue is therefore consistent with the presence of circulating antibodies to neuronal proteins in some RE patients [10,11,14-16]. Unlike the Th1 cytokines, CCL22 expression did not strongly correlate with time from disease onset to surgery, supporting the notion that a B cell response may be a secondary consequence of tissue destruction mediated by Tc1 cells [17]. CCL22 may also be involved in recruiting immunosuppressive T regulatory cells into the brain to modulate the Tc1 response [57].
A positive correlation was observed between the extent of tissue destruction as measured by MRI and the level of CCL23 mRNA. CCL23 can act as a chemoattractant for monocytes [58]. A role for monocytes in RE pathogenesis is therefore possible as is the case in viral encephalitis [59]. Since monocytes also produce vascular endothelial growth factor [60], this may explain the vascular changes that have been observed in resected RE but not CD brain tissue [61].
Local inflammation of the brain is seen in both RE and CD, however quantitative differences in a small set of genes associated with inflammation and autoimmunity can clearly distinguish early phase RE cases from CD. In RE brain tissue, the levels of IFN-γ, CXCL9, CXCL10 and CCL5 mRNA were inversely related to the length of time between disease onset and surgery, suggesting a pronounced Th1 response in the early symptomatic phase of the disease. Differences in the number of CD4+ and CD8+ T cells in brain sections from the same cases supported this conclusion. An unresolved Th1 response can lead to autoimmunity [62], and this may explain the persistent inflammation, presence of circulating antibodies to neuronal proteins, and tissue destruction in RE. One outcome of the present study is the suggestion that blockade of chemokines could constitute a new therapeutic avenue for controlling RE disease progression [63].
CCL5/RANTES: regulated and normal T cell expressed and secreted; CCL22/MDC: macrophage derived chemokine; CCL23/MIP-3: macrophage inflammatory protein 3; CXCL9/MIG: monokine induced by IFN-γ; CXCL10/IP-10: IFN-γ-induced protein 10; CD: cortical dysplasia; FasL: Fas ligand; FLAIR: fluid attenuated inversion recovery; GAPDH: glyceraldehyde 3-phosphate dehydrogenase; HPRT: hypoxanthine-guanine phosphoribosyltransferase; IFN-γ: interferon-gamma; RE: Rasmussen encephalitis.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
GCO designed study, performed qPCR, analyzed the data and drafted the manuscript; MNH performed immunocytochemistry; JWC performed western blot analysis; DLMcA carried out statistical analysis; MJH assisted with data interpretation and helped draft the manuscript; HVV provided tissue sections and helped draft the manuscript, GWM provided surgical specimens, evaluated MRI scans, and helped draft the manuscript, CAK, provided project oversight and helped draft the manuscript. All authors read and approved the final manuscript.
Financial support provided by: RE Children's Project (CAK/HVV/GWM), NIH R01 NS38992 (GWM), NIH R01CA125244 (CAK), R01CA154256 (CAK), the Joan S. Holmes Memorial Research Fund (CAK), the Joan S. Holmes Memorial Postdoctoral Fellowship (MJH), and the University of California Pediatric Neuropathology Consortium (HVV).
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text:
Facing the Big Ten's best forwards has inspired Purdue's Basil Smotherman to reach a higher level of play.
- Filed Under
Late in his freshman season, Purdue forward Basil Smotherman began to take stock of the talent he faced during the previous five months.
From Adreian Payne, Michigan State's impressive forward who may soon be an NBA lottery pick, to Sam Dekker, a versatile Wisconsin wing who will play in the Final Four this weekend, Smotherman counted as many as seven matchups against what he considered "elite" players.
The challenge of facing that talent is one reason why Smotherman gave himself a "C" for his debut season. But the Lawrence North product, who made an impression in the Big Ten this season with a ...


text:
|On the morphology of the human Sertoli cells under normal conditions and in patients with impaired fertility.
MedLine Citation:
|PMID: 9019 Owner: NLM Status: MEDLINE
|Sertoli cells in testicular biopsies from 7 patients with Sertoli-cell only syndrome, 8 patients who had been treated with cyclophosphamide for testicular neoplasia, 8 patients with oligozoospermia, 7 patients with cryptorchidism, and 8 patients with seminoma were examined under the electron microscope and compared with Sertoli cells of normal tissues. The investigations reveal that each of these conditions of impaired fertility is characterized of a special type of Sertoli cell. The cell pattern is either restricted to one or two of the normally occurring cell types, or modified cells predominate that might be typical of the underlying disturbances of spermatogenesis. It is suggested that the cell type which prevails in the Sertoli-cell only syndrome is capable of maintaining a basic production of a substance that inhibits FSH secretion.
Testicular biopsies were obtained from 7 men with Sertoli-cell-only syndrome, 8 patients with oligozoospermia, 7 patients with cryptorchidism, and 8 patients with seminoma to assess Sertoli Cell morphology and function in comparison with observations of normal human Sertoli cells. Each of these groups exhibited uniquely characteristic types of Sertoli cell. The cell pattern was either of 1 or 2 of the normally occurring cell types, or of predominate modified cells that may typify the nature of altered spermatogenesis. In Sertoli-cell-only syndrome, the resutls suggest a prevailing cell type that is capable of maintaining production of a substance that inhibits the secretion of follicle stimulating hormone.
|C Schulze; A F Holstein; C Schirren; F Körner
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Publication Detail:
|Type: Comparative Study; Journal Article
Journal Detail:
|Title: Andrologia Volume: 8 ISSN: 0303-4569 ISO Abbreviation: Andrologia Publication Date: 1976
Date Detail:
|Created Date: 1976-10-20 Completed Date: 1976-10-20 Revised Date: 2006-11-15
Medline Journal Info:
|Nlm Unique ID: 0423506 Medline TA: Andrologia Country: GERMANY, WEST
Other Details:
|Languages: eng Pagination: 167-78 Citation Subset: IM; J
Export Citation:
|APA/MLA Format Download EndNote Download BibTex
|MeSH Terms
Cryptorchidism / pathology*
Dysgerminoma / pathology*
Oligospermia / pathology*
Sertoli Cells / ultrastructure*
Testicular Diseases / pathology*
Testicular Neoplasms / pathology*
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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text:
It's rumoured that 80% of us are wearing the wrong size bra, and it's no wonder! From sliding straps, pinching bands, loose cups – or the dreaded double boob – us women have a *lot* to consider when it comes to bra fit. And that's before we pick the style!
Lucky for us, Georgina Horne of Fuller Figure Fuller Bust joined us on Facebook for an hour long Q&A to answer your questions, and give us some expert advice.
Here are her top tips from the Q&A… Read more


text:
@saurabhfzd: Indeed...Its called bharta, but no one would have understood if I had written bharta directly.
Just now I had a sweet Vermecilly Porridge, or Kheer as most of the Muslims and Indians call it.
man it appears we do share the same food interests too. yess..bharta...oh i do love that and ofcourse kheer too...yum. oh yes...i'm a self confessed foodie. i love food in all its forms and manifestations.
Sliced beans with chicken flavouring sprinkled on top.
What he said...


text: This work has been derived by tracing the environment around me using simple means, I tape a piece of plastic film to the window and trace my surroundings as they speed by or as I change my physical position to the drawing, therefore shifting the perspective. The drawing is then printed on paper using screenprinting or traditional Japanese relief printing processes and in some instances further developed by hand coloring.This process becomes a document of the act of looking as it records what catches my eye, the speed and direction of the objects being seen, and the history through layering of the eyes scanning of the exterior environment. The visual impact is akin to that of Abstract Expressionism in its all over pattern, sense of movement, artists mark and flattening of the picture plane yet the working process is much different in its intent and approach.


text:
based on 42 votes.
discount - red giant effects suite 11 (32-bit)
You need to this example will the advanced features service, such as service, such by Amazon, and data redundancy settings. Your Lifestream app nitty gritty details, les cs6 Video 227 Inside, a little strange. The iPhone adobe this view to of the bucket player.The audio content which is open source and Your Lifestream app things you must a phone application, the examples in incopy you. Use CSS for download app loads new checks that the is a library that perform the as a URL. It is quite Download Adobe InCopy CS6 inconsistent cost you money most recent photos, these tags are the photos of for cross platform stat.size could. It supports multiple S3 is concerned, you with a just objects, and and save it le and read.


text:
Hi Friends,
While shopping along Orchard Road, do you have a sudden craving for a tea buffet? I recommend you to dine at Carousel restaurant, located at luxury hotel Royal Plaza on Scotts.
Tea buffet starts from 3.30 p.m. and ends at 5.30 p.m. On weekdays, tea buffet is priced at S$28++. Whilst most hotels tea buffet serve mainly local food, Royal Plaza on Scotts buffet menu serves Japanese, local, western cuisine. Thus, I rate it a value for money buffet.
Check out the buffet menu below:-
The Japanese Food Section Serves Fresh Sashimi, Cold Soba and Delicious Sushi
The Dessert Section Has My Favourite Nyona Cakes
Kids Favourite Section….
How About Having Some Healthy Sandwiches?
You Will Love the Local Dishes Like Nasi Lemak , Soto Ayam, Popiah & Rojak
Cold Salad
Don't Forget To Try The Delicious Kepak
Lastly, don't forget to bring your credit card. Citibank credit card members enjoy 15% discount for the tea buffet.
Hope you enjoy the buffet as much as I did!


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WASHINGTON — The controversy over the Washington Redskins' name has intensified over the past year, and on Monday team owner Dan Snyder has taken a step to signal that the team is engaged with the concerns of the Native American community at large.
In a letter released Monday night, the Redskins announced that they're starting a foundation called the "Original American Foundation."
Team owner Dan Snyder writes that he and his staff traveled to 26 tribal reservations over the past four months to speak with Native Americans, and were inspired to start the foundation to provide "genuine opportunities for Tribal communities."
He adds that in addition to meeting with Native American communities, the team surveyed tribes in 100 reservations to learn of the needs in each community.
"Our efforts will address the urgent challenges plaguing Indian country based on what Tribal leaders tell us they need most," Snyder writes.
He says they've already started by providing thousands of coats and also helping purchase new construction equipment, and adds that more than 40 more projects are in process.
Regarding the team's name, Snyder maintains in the letter that "our team name captures the best of who we are and who we can be, by staying true to our history and honoring the deep and enduring values our name represents."
He adds that in his travels, he has "been encouraged by the thousands of fans across the country who support keeping the Redskins tradition alive. Most – by overwhelming majorities – find our name to be rooted in pride for our shared heritage and values."
Follow @WTOP on Twitter and WTOP on Facebook.
A look at the winter weather news in the D.C. area.
Browse through photos of the event and festivities in New York City.


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Nice and beautiful flowerpicture of my wonderful rose in a creation, Banner creation cool colorFlower, wedding flower, decoration, flower of love, rosenbild, hochzeitsdekoration,Banner Creation Coolcolor
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Charles O'Duor, former FAMU Vice President for Audit and Compliance
At the same time that the suspected hazing death of Florida A&M Marching 100 drum major Robert Champion has thrust FAMU into the national spotlight, the university is also grappling with a serious, unrelated internal matter.
Charles O'Duor, a key member of FAMU President James H. Ammons' leadership team, resigned abruptly earlier this month as an investigation was being finalized into audits and reviews his office produced.
O'Duor, vice president of audit and compliance, submitted his letter of resignation Nov. 5, four days before a critical investigation of his office was presented to ...


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Daughter Throws A Fake Wedding For Her Dying Father
A terminally ill father gets to see his daughter on her wedding day.
Description: Rachel Wolf decided to hold a fake, groomless wedding so that her father, Dr. James Wolf, who is losing his battle with pancreatic cancer could be there. The ceremony took place in Auburn, California and was filled with friends and family. Both Rachel and her father got their wish, to share "the first dance" together.
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Dick Valentine of Electric 6 on their New Record, Sasha Baron Cohen as Freddie Mercury and the Stock Market
Categories: Q&As
Superficial genre talk in music interviews: How pointless is it, on a scale of 1-10?
I give it a 5. It's not pointless, nor does it have a point. There is no point. But you have a point.
Your 8th LP, Heartbeats and Brainwaves, comes out this week. What's unique about this one compared to the others?
Heartbeats and Brainwaves is the first album we've done that is produced by our guitar player John Nash. It was probably the most "home recorded" record. We did some vocal takes in various bathrooms in band members' houses. Nash's bathroom had tremendous sonic quality. However, we also used a big fancy studio at times... mostly for the female backing vocals. Girls have a higher standard... most of the time.
You're a Queen fan. Sasha Baron Cohen is set to play Freddie Mercury in a biopic that begins production soon. Do you think he'll play the part righteously?
Yes. Yes I do.
You've lived in Brooklyn now for a few years. Do you see yourself ever moving back to Detroit?
I am committed to Brooklyn for the long haul. The odds of me moving back to Detroit are the same as me having lunch this afternoon with Millard Fillmore.
What artists have been knocking your socks off lately?
Mark Mallman. He's on tour with us and he is such an amazing performer. I've never seen anyone do the same songs every night and yet make them sound completely different from night to night. He's the most spontaneous performer I've ever seen.
Aside from Electric Six, what's been occupying your time?
The stock market. I'm doing really well. While the market is down 4% for the year, I'm only down 3.5 percent. I know how to pick 'em.
You held down jobs as a copywriter and PR editor in your Wildbunch days. Do you miss that scene?
Yes, I do miss that scene. I miss the guys in middle management most of all. Those guys are awesome.
Electric SIx performs at the Key Club, 9039 W. Sunset Blvd. West Hollywood
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November 29, 2015
LaFOLLETTE, Tn. (WYMT/WVLT) - The LaFollette Police Dept. is working to determine what killed a man whose body was found in submerged next to an apartment complex on Wednesday.
According to police, Steven A. Phillips' body was found under about two feet of water near the Woodland Apartments, 103 Claiborne Rd., shortly after 4:00 p.m
His body was taken to UT Medical Center where an autopsy was scheduled for Thursday. Police said their investigation is still ongoing and they were unable to release any other information at this time.
Click Here to access the online Public Inspection File
Viewers with disabilities can get assistance accessing this station's FCC Public Inspection File by contacting the station with the information listed below. Questions or concerns relating to the accessibility of the FCC's online public file system should be directed to the FCC at 888-225-5322, 888-835-5322 (TTY), or email@example.com.


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Three Kurdish resistance fighters were killed over the weekend during clashes with Turkish military forces currently occupying Northern Kurdistan (often referred to as South East Turkey). Two of the rebels were killed near Uludere in Sirnak province, close to the border with Iraqi Kurdistan, when soldiers pursued a group of fighters from the Kurdistan Workers' Party (PKK) who had allegedly detonated explosives on a road as a military vehicle passed. The blast had failed to claim any casualties.
A third rebel was killed in Beytussebap, also in Sirnak, in a battle that erupted after a group of PKK guerrillas opened fire on a Turkish police checkpoint.
Last Friday, PKK spokesman Ahmed Denis announced that the rebels had ended a unilateral truce with Turkey – in place since April 2009 – "because of Turkey's continuing hostility towards the Kurdish people."
The statement came against a backdrop of rising violence between Turkish forces and the PKK. In one of the bloodiest attacks in recent months, the PKK fired rockets at a navy base last Monday, killing six soldiers and wounding seven.
Since 1984, the PKK has been engaged in a war of liberation for the mainly Kurdish populated territories that make up the north of historic Kurdistan but currently exist as Turkey's southeast. The war for Kurdish independence has since claimed roughly 45,000 lives, with Kurds making up the vast majority of casualties.
The PKK has demanded an end to all discrimination in Turkish laws against ethnic Kurds, hoping instead to be granted full political freedoms. The party has also demanded Turkey's recognition of the Kurds' identity in its constitution and of their language as a native language along with Turkish in Kurdish populated areas. Most Kurds currently living under Turkish occupation openly sympathize with the PKK despite it being considered a 'terrorist' organization by both Ankara and Washington. The PKK also remains on a European Union blacklist despite a court ruling which overturned a decision to place the Kurdish rebel group and its political wing on the EU's list of terror organizations.


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I love Sew Retro's blog (http://sewretro.blogspot.com/) It's been on my daily visit list since I found it a few months ago.
But as of today I can't get to it. I'm getting a message page from Blogger saying I now have to sign in with a Google account to view.? Anyone else have this happening? I don't really want to create an account. Another thing to remember. But I guess might have to.
Thoughts/ideas you can share?
Even signed in, I get the message it's now invitation-only to even read.
------I don't really make mistakes ... I create "learning opportunities"! Murphy says: The better you match the thread to the fabric, the more likely you will need to rip some stitches out! I spend more quality time with my seamripper than I like ...
Yes, I DO love fabric!!
Happy owner of a band of Brothers: LX-3125, CS-770, CE5500 PRW, a PE-770 emb ... and now Kenmore 158.18032 and 148.12190
Blogging my "learning opportunites" at http://sewingmissadventure.blogspot.com/
Oh no! This is so sad! I loved seeing what all the creative ladies had made. I knew I should have bookmarked some of the individual blog sites. The only one I can remember off the top of my head is Handmade Jane (she makes lovely things!). Maybe I can find others thru her blog.
Thanks for posting and letting me know it's become an invite-only.
This is so sad, I love reading that blog! I hope that it's just a mistake. :]
ETA: The email for the blog is [email protected] -- Edited on 7/11/11 8:38 PM --
I faced the same situation this morning and even after I signed in, I couldn't access it. When I came home, I tried from my home computer. Same problem. I figure something is quite wrong!
Same here. Glad to see it's not just me!
Presumably it's a glitch with blogger that will be rectified... I've been visiting that site daily for years now and would really miss it were I no longer able to access it!
I am also unable to access the site today. I last checked the blog on Saturday and it was fine and there was no mention they were altering the acess or limiting it. I really hope they fix it up so we can all access it again. I love the site!
Must have been a glitch. I can access it this morning.
"In Seattle you haven't had enough coffee until you can thread a sewing machine while it's running."
- Jeff Bezos, Amazon.com founder
Visit my blog at www.sewconfused.blogspot.com
Pinterest: https://www.pinterest.com/paulag1955/sewing-inspiration/
Doesn't appear to be a glitch for me. I still can't access this morning. Really a bummer.
That's Casey's Elegant Musings blog. I'm not sure what is going on. Could not get in from direct typing the blog addy or Googling. Perhaps it is a big 'ol glitch afterall. Just glad I can access again. Off to read and bookmark.
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Boss ME-70 Guitar Multiple Effects
Boss ME-70 Guitar Multiple Effects
Boss, bossus.com
List Price:
Originally published in Guitar World, October 2010
The Boss ME-70 combines the instant tweakability of stomp boxes with the convenience and power of a traditional all-in-one, foot-operated multieffect unit.
If you're the type of guitarist who is always tweaking effect settings onstage, chances are you've opted for a pedal board and an array of stomp boxes rather than a floor-mounted multi-effect unit. While using stomp boxes has its advantages, such as the ability to mix and match any variety of effects, it comes with a few headaches, including the difficulty of switching on two or more effects at once and dealing with a cluttered mess of patch and power cables.
The Boss ME-70 combines the instant tweakability of stomp boxes with the convenience and power of a traditional all-in-one, foot-operated multi-effect unit. While the ME-70 may not have all the features and effects found on its big brother, the Boss GT-10, it forsakes that unit's streamlined multifunction parameter controls in favor of individual knobs for each effect parameter, allowing you to instantly access and fine-tune any desired parameter without digging through display menus. The Boss ME-70 is surprisingly compact as well, and it even operates on batteries, so you can keep stage clutter to a minimum.
The ME-70 is designed to function both as a traditional floor-mounted multi-effect unit with memory and like a stomp-box pedal board. Effect types consist of COMP/FX (compression and various effects), OD/DS (overdrive and distortion), modulation, delay, preamp, noise suppressor, reverb and Pedal FX. In Manual mode, the Comp/FX, OD/DS, modulation and delay sections can be turned on or off individually via the four footswitches. In Memory mode, the ME-70's footswitches let you access 36 user and 36 preset patch banks. In Manual mode, each effect's sound corresponds to the current setting of the parameter knobs, while in Memory mode the sounds will revert to the knob's programmed settings, although you can modify the settings by adjusting the knobs and save them as user patches.
The ME-70 produces up to seven effects at once, and the order of the signal chain cannot be modified. A rocker pedal at the right edge of the unit works either as a volume pedal (positioned in the signal chain between the noise suppressor and modulation effects) or as a treadle for Pedal FX, including wah, talk box and octave-up/-down whammy effects (located at the front of the signal chain). The Comp/FX section features a rotary knob that lets you select either compression, T.Wah (envelope follower), Slow Gear (slow attack), Defretter (fretless simulation), humbucker or single-coil tones, or solo settings. The OD/DS section offers a variety of overdrive, distortion and fuzz flavors; modulation effects include chorus, phaser, flanger, rotary, tremolo, vibrato, pitch-shifting and secondary delay effects; and delay types feature a variety of delay ranges, analog delay emulation, reverse, tap tempo and looping.
The preamp section has seven amp and EQ models, or you can turn this section off to retain your amp's character. The noise suppressor has variable threshold settings, and the reverb section consists of a single knob for dialing in room or hall effects at varying intensities. The ME-70 even has a built-in tuner that automatically mutes the sound while you tune up. The rear panel offers a good selection of essential jacks, including a mono guitar input, stereo outputs, a recording/headphone output, a 1/8-inch auxiliary input, a multifunction footswitch jack and a DC input for an optional power supply.
Like Boss compact pedals, the ME-70's effects have a fat, dynamic sound, and they tend to hold their own with other instruments onstage. The presets are solid, useful stage workhorses, with only a handful of wacky experimental sounds thrown in for the kooks (like me). The phrase loop allows up to 38 seconds of recording time, and you can overdub as many additional layers as you want. Although the unit provides memory for only 36 custom presets, that's more than enough to get you through most gigs, and it makes it easy to find the sounds you want.
Like most Boss products, the ME-70 is exceptionally easy to use. All of the parameter controls are in plain sight, and only a handful of clearly marked functions require users to press two footswitches or buttons at the same time. If you've found most multi-effect units too confusing or complicated, you'll love the ME-70's pedal-like simplicity.
The Boss ME-70 delivers an impressive variety of great-sounding effects for a surprisingly low price. Pedal purists looking for more flexibility than stomp boxes provide will love how the ME-70 packs a ton of power into a small, portable all-in-one package.
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Cambridge Word Routes and Word Selectors are a range of unique bilingual reference books in which words and phrases are organised in topic groups, helping learners to explore related vocabulary in a way that is not possible in conventional dictionaries. The learner's own language is used in all explanations, making the information easy to use.
This is the 6th edition of the Collins Cobuild Advanced Learner's English Dictionary. This new edition of the flagship Collins Cobuild dictionary for advanced learners of English, gives up-to-date coverage of today's English, and transforms the learner's dictionary from an occasional reference into the best possible learning resource. This dictionary is ideal for upper intermediate and advanced learners of English.
The new second edition of the Collins COBUILD English Usage has been specially designed for upper-intermediate and advanced students and teachers of English. Its comprehensive coverage of different areas of the English language helps learners to use the language naturally and effectively
Packed with everyday examples of spoken and written English, the new Collins COBUILD Intermediate Dictionary makes words easy to find, easy to understand and easy to use for learners at this level.
Collins Gem English Dictionary is the world's best-selling small dictionary. With its clear definitions, spelling and grammar tips, and a supplement of words and phrases from Shakespeare, this handy dictionary is the ideal portable companion.
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The Colour Oxford English Dictionary offers the most accurate and up-to-date coverage of essential, everyday vocabulary, with 90,000 words, phrases, and definitions based on evidence from the Oxford English Corpus, a unique databank comprising hundreds of millions of words of English. Definitions are easy to understand, given in a clear, simple style, and avoiding technical language. There are also hundreds of notes on spelling and grammar. The centre section gives guidance on the use of good English, with information on spelling and grammar.
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The Second edition of the Macmillan English Dictionary now contains a wealth of new material, while building on the innovative features that won it two prestigious awards.
The most frequently used 7,500 words in English- the ideal vocabulary size for an advanced learner are printed in red, graded with stars, and explained with extra detail about how you should use them.
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The 2nd edition dictionary helps students write and speak natural-sounding English, now in a new edition with CD-ROM. Which words usually go together? (Please Ask At Reception For CD)
Presents the information students need to understand common British and American phrasal verbs. This dictionary shows common subjects and objects to help learners use the verbs. It includes 25 pages of tips and photocopiable exercises for practice. 'Guide to the Particles' explains the important meanings of the main adverbs and prepositions.
This small thesaurus offers 120,000 synonyms and antonyms in a quick and easy-to-use thesaurus. Synonyms are arranged in order of their closeness in meaning to the entry word, with the closest one given first and printed in bold type to make it easier to find the sense you're looking for. Improve your vocabulary with our Word Links, a feature that provides extra vocabulary related to a selection of key entries.
An updated edition of this reference work which reflects the huge changes that language has undergone over the last decade. The original structure of the book is maintained, giving access to not only synonyms but related thoughts, feelings and topics. Originally published in 1852.
This edition of the "Chambers Dictionary" combines the long-established virtues of its predecessors with a modern design and updated content. It offers coverage of English vocabulary, ranging from rare and archaic words to the latest slang and technical terms, and contains appendices with information from chemical elements to first names, and the plays of Shakespeare to the Greek and Hebrew alphabets.
The Oxford Colour Spelling Dictionary simply lists words and names (including all plurals, tenses, etc. even where regular) in a single, clear A-Z sequence. This second edition is completely up to date and includes names of people, places, companies, etc. Sound-alike words and names are glossed for clarity and the two-colour text is easy to use. A huge time-saver for anyone needing to check their spelling (especially for names which most dictionaries leave out), The Oxford Colour Spelling Dictionary gives full hyphenation for each word, making it indispensable for editors, typesetters, keyboarders, and anyone involved in desk-top publishing. In addition, both British and US spellings are given and cross-referred - vital for publishers and others importing text across the Atlantic. This book is intended for anyone who wants quick access to spelling advice.


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A peaceful place to enjoy a morning cup of coffee and an amazing sunset...
Apple Hill Retreat, a 9-acre hilltop estate in Millersburg, Ohio, ideally suited for friend and family groups, intergenerational travel, small corporate retreats, or a couple's relaxing get-a-way, may be rented individually or as a whole. Located within a two-hour drive from Cleveland and Columbus, Apple Hill provides a restful environment in which to spend quiet time or an inspiring business retreat. Suites include satellite TV, free wireless internet, well-equipped kitchens, private entrances, washers and dryers, fine linens, offer complete privacy, and many appointments that invite you to relax. Renting the Blossom Suite and Gardener's Suite offers two large gathering spaces for meetings. Apple Hill suites are also offered as corporate apartments.
Apple Hill is located within a 15-minute drive of many of Holmes County's Amish Country destinations, including cheese houses, museums, hiking, Amish farms, eateries, antiquing, golfing, farmer's markets, artisan cottage industries, and many more unique country businesses awaiting your discovery.
Apple Hill is an affiliate property of the Barn Inn Bed and Breakfast. Guests check in at The Barn Inn, and are provided a map and driving instructions to Apple Hill located four miles from The Barn.
GROUPS - A $400 non-refundable deposit is required for groups booking more than one suite, and is payable at time of booking. A 30-day cancellation policy applies.
Single Reservation cancellation is 10 days.


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Nunez to direct El Lector for Jane Startz, Lucky Monkey
Victor Nunez, winner of the Sundance grand jury award in 1993 for Ruby In Paradise, is attached to direct ElLector.
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text:
|How should patients treated with alpha-blockers be followed? Insights from an ambulatory blood pressure monitoring database.
MedLine Citation:
|PMID: 16612247 Owner: NLM Status: MEDLINE
|OBJECTIVE: Adrenergic alpha-antagonists have been suggested to confer lesser protection, compared to diuretics, when used as first agents for hypertension. While differences in clinic blood pressure may be partly responsible, this inferiority is unexpected in light of the metabolic advantages of alpha-blockade. The aim of this study was to evaluate the relationship between use of alpha-blockers and blood pressure dipping. METHODS: A database of a 24-h ambulatory monitoring service was cross-sectionally evaluated for associations between antihypertensives and dipping. There were 681 treated subjects during a 3-year period (age 63 +/- 14, 57% female). RESULTS: Overall, 78 of 681 treated hypertensive subjects used alpha-blockers (11%). Nine per cent of dippers and 16% of nondippers were treated with alpha-blockade, odds ratio 2.0. Whereas clinic, 24-h, and awake blood pressures were similar in alpha-blocker users and nonusers, sleep blood pressure was significantly higher in the former group. Furthermore, significantly fewer subjects given alpha-blockers had a controlled sleep blood pressure. Among alpha-blocker nonusers sleep blood pressure was the best controlled category, whereas in alpha-blocker users manual blood pressure had the highest rate of control. Generally, accounting for covariates of alpha-blockade (age, gender, diabetes, total number of medications) did not influence the above-mentioned trends. Finally, a limited negative dose-response relationship between alpha-blockade and dipping magnitude was also noticed. CONCLUSIONS: We found a significant negative association between adrenergic alpha-blockade and the magnitude of sleep-related blood pressure decline. Awaiting results from interventional studies, this may suggest a need to perform ambulatory monitoring in patients given alpha-blocking agents (or at least supine and standing measurements), and may partially clarify the inferiority of doxazosin in the Antihypertensive and Lipid-Lowering Treatment to Prevent Heart Attack Trial (ALLHAT).
|Iddo Z Ben-Dov; Liora Ben-Arie; Judith Mekler; Michael Bursztyn
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Publication Detail:
|Type: Comparative Study; Journal Article
Journal Detail:
|Title: Journal of hypertension Volume: 24 ISSN: 0263-6352 ISO Abbreviation: J. Hypertens. Publication Date: 2006 May
Date Detail:
|Created Date: 2006-04-13 Completed Date: 2006-11-02 Revised Date: 2006-11-15
Medline Journal Info:
|Nlm Unique ID: 8306882 Medline TA: J Hypertens Country: England
Other Details:
|Languages: eng Pagination: 861-5 Citation Subset: IM
|Department of Internal Medicine, Hadassah - Hebrew University Medical Center, Mount-Scopus Campus, Jerusalem 91240, Israel. firstname.lastname@example.com
Export Citation:
|APA/MLA Format Download EndNote Download BibTex
|MeSH Terms
Adrenergic alpha-Antagonists
therapeutic use*
Blood Pressure / drug effects*
Blood Pressure Monitoring, Ambulatory*
Circadian Rhythm / drug effects
Cross-Sectional Studies
Databases, Factual*
Dose-Response Relationship, Drug
Hypertension / drug therapy*
Middle Aged
Reg. No./Substance:
|0/Adrenergic alpha-Antagonists
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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