text: The Saturday Show at Festibelly - Cribsz By Colin and Matt We took Colin and Matt from The Saturday Show to Festibelly 2011, gave them a... more »We took Colin and Matt from The Saturday Show to Festibelly 2011, gave them a backstage pass and a camera and let them loose. In this video we find out how they live festival style. The Saturday Show is broadcast every Saturday on Amazing Radio from 9am-12pm. You can follow Colin and Matt on Twitter @colinandmatt or become a fan on Facebook.com/colinandmatt Music Lerix - I Got This http://www.amazingtunes.com/users/feelthelerix Arty McFly - The Thrill http://www.amazingtunes.com/users/artymcfly « less Published August 30, 2011 13 views More Info » Full Credits Colin and Matt Uploader Stats & Data 0 Funny Votes 0 Die Votes 13 Views Published August 30, 2011 Categories: Sketch Clean Comedy Parody Keywords: festibelly 2011 amazing radio colin and matt the saturday show cribs parody comedy funny spoof sketch humor Up Next: Carl & Chet sell fitness AUTOPLAY Up Next In: 10 Carl & Chet sell fitness The Sauna Suit Behind The Microphone - A potato addiction MTV Cribs Parody Follow Facebook Twitter Tumblr Instagram Browse Related Comments 5+ more View All ▶ Colin and Matt Vaguely Related Videos Vaguely Related Pics & Words Advertisement Advertisement


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Celebs Out & About: Rihanna
Sultry singer Rihanna rang in the New Year with her reported lesbian lover Melissa Forde and friends at Do Brazil restaurant at Shell Beach in Saint Barts. Rihanna's fans are happy that Rihanna cleaned up her act and lived to see 2015.
Plush Blue Entertainment's New Year's Eve Party
Atlanta megastars Tip "T.I." Harris and his lovely wife Tameka "Tiny" Harris rang in the New Year at Plush Blue Entertainment's New Year's Eve party at Vibe Restaurant and Lounge in Atlanta! If you weren't there, you missed one of the most talked about New Year's parties in the A! Also in the house was rapper/philosopher David Banner, and a blogger you know very well.
Above: Friend of the blog Omar "Slim" White, right, posed with David Banner, left, and T.I., center.
Celebrities Celebrating the New Year: Monica, T.I. and Tiny, LeBron and Savannah, Ludacris and Eudoxie
R&B Diva Monica celebrated New Year in Miami with T.I., Tiny, socialite La La, rapper Future, BoB, producer Rico Love and others.
T.I. and Tiny celebrate the New Year in Miami
Rapper Tip "T.I." Harris and his wife, Tameka Harris, celebrated the New Year at Cameo Nightclub in Miami. Other celebs in the house included rappers Future and BoB.
Photos: Thad McAdams/ Exclusiveaccess.net
More from Sandrarose.com:

subset: commoncrawl


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Artist: Grand Funk Railroad
Song Title: Carry Me Through
To start my search for tomorrow.
On and on,
There's just no end to the sorrow.
But I designed a way,
To see my life through pity.
I don't have too much to say,
And the tears won't let me be.
Carry me through the hard part.
Help me find a new start.
Carry me through the hard part.
Far too long,
I've thought of all the excuses.
Try to go on,
And stay my time to an end.
Dreams in my head,
All turning dark and useless.
Help me find the hand,
That knows where I've been.
Ahhhhhh ...
Ahhhhhh ...
Carry me through ...
Carry me through ...
Carry me through ...
Carry me, carry me, carry me ...
From http://www.lyrics007.com


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153 Views 2 Replies Latest reply: Nov 15, 2012 6:46 AM by lllaass
AthenaMuze Nov 14, 2012 8:12 PM
Re: music won't stay synced in response to AthenaMuze
Couple of clarifications:
1. No audio at all excepting podcasts
2. Yes I have checked my sync settings, music is checked, always has been
lllaass Nov 15, 2012 6:46 AM
Re: music won't stay synced in response to AthenaMuze
- Reset the iOS device. Nothing will be lost
Reset iOS device: Hold down the On/Off button and the Home button at the same time for at
least ten seconds, until the Apple logo appears.
- Unsync all music and resync
- Reset all settings
Go to Settings > General > Reset and tap Reset All Settings.
All your preferences and settings are reset. Information (such as contacts and calendars) and media (such as songs and videos) aren't affected.
- Restore from backup. See:
- Restore to factory settings/new iOS device.


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View Single Post
December 20 2011, 04:18 PM
Location: The Barmuda Triangle
Re: Dexter
I thought this season was pretty good, probably about on par with Season 3, which was the previous worst "good" season. I wish they hadn't left that thread dangling with intern-guy-whose-name-I-still-don't-know. I was confused by the ending because I thought Deb had told Dexter to go do a sweep of the HOUSE where they found Travis's last painting not the chuch so I was confused why she had shown up there. I guess I was wrong though and she had said the church, which doesn't make a lot of sense in the context of the storyline, and seems pretty careless of Dexter to use as his kill site (but then, his judgment has been pretty off for a few seasons now). Ugh. Anyway, despite that last bit of bad writing, I thought it was a pretty good season and not as gratingly stupid as the 5th one.
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This is Caiden Cowger: He has his own radio show and everything! Which seems cute… until you realize he's a young gay basher. When you listed to his twice-weekly internet talk show based out of West Virginia, you realize that Caiden doesn't like gays. Not at all. Homosexuality is a belief. It is a– it [Read More...]
A Rush Limbaugh For the Next Generation
Says Pastor: This is a Christian Nation and Atheists Should Move Out
Pastor John Hagee was feeling the Christian love this past Sunday. He was reflecting on the sacrifices our soldiers made on D-Day, which happened 68 years ago tomorrow, when he thought it was a perfect moment to call out the atheists. They're saying that the 10 Commandments have been taken out of the courtrooms and out of the schoolhouses [Read More...]
Atheist(s) Don't Exist… God Said So
Sorry guys. Pack it up. We are apparently a figment of someone's imagination. Or exist in a snow globe like on that show I'm too young to have watched. Blogger Ralph Barker just wrote an article entitled "Atheist Don't Exist-God Said So". I don't know about you guys, but that's all the evidence Ineed to know that [Read More...]
Are They Sure They Want To Brag About This?
This picture has been going around on the Facebooks: Just a few quick thoughts: Inappropriate use "of" quotation marks is one of my favorite things. Really? Tuition is free? What happened to tithing? The "winning souls" thing makes me vaguely uncomfortable. As if I'm the assignment they need to work on. Does anyone spellcheck these things before [Read More...]
Who is Whining About Contraception Now?
Surprise! It's the Catholics, Lutherans, and Presbyterians in Orlando, FL! Bishop John Noonan thinks that insurance covering contraception and sterilization is an "affront to [their] religious beliefs": The payment of our insurance premiums will also subsidize abortion inducing drugs even though we do not believe in this culture of death. *Cue thunder clap* Culture of death?! [Read More...]


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Product Details
Wireless Back-Up Camera For RoadMate 5255/5265/9250/1700/9055/9165/RV9145/RV9165 GPS Navigators (CPBCKUSGXXX) by Magellan®. The Award-winning Magellan Wireless Back-up Camera is the perfect companion for your Magellan GPS navigator. (please verify compatibility)
- Wireless Camera Mount- No need to run wires throughout your car
- Extended 45 foot Range- Great for most Cars, Trucks, Vans, RVs and Small Trailers
- Adjustable to 45 degrees- Great for mounting on Trucks, RVs and SUVs
- Wide-Angle View- Expansive 120° horizontal and 100° vertical viewing area
- Automatic Switch to Rear-View Mode- Put your car in reverse and your navigator will automatically switch to rear-view mode
- Camera is Dustproof and Waterproof- Rated IP67, camera is waterproof and dustproof
- Transmitter is Weatherproof- Rated IPX4, transmitter is water resistant
- Mirror Output from Camera- Plug into compatible mirrors and see an enhanced perspective
- A/V Input- Compatible with video systems that have a 3.5mm A/V input
When you put your car in reverse, your Magellan navigator will automatically switch from navigation mode to rear-view mode, allowing you to see what is behind your car – including children, pets and other cars.
The substantial 45 foot wide-angle viewing range gives you added awareness to prevent accidents and property damage. Parking your car is much easier with the Magellan Wireless Back-up Camera, giving you a much better sense of proximity to objects behind you. Both the Wireless Camera and the Transmitter have been tested and rated to withstand bad weather and harsh conditions; the transmitter is water resistant and the camera is waterproof and dustproof.
The Back-up camera can be connected to compatible mirror systems as well as compatible video systems that have a 3.5mm A/V input.173.23Brand: Magellan Part Number: CPBCKUSGXXX UPC: 763357126365
Magellan brand allows people to travel, work and play their way with leading portable navigation and positioning solutions across multiple consumer markets. Recognized as an industry innovator, the company is the creator of the award-winning Magellan RoadMate and Maestro series portable car navigation systems, as well as the Magellan Triton outdoor handheld navigation devices. MiTAC Digital Corporation is privately held and headquartered in Santa Clara, Calif. The company is a wholly-owned subsidiary of MiTAC International Corporation and continues to operate under the Magellan name and logo.
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You will never pay more with CARiD! Not only will we match prices from any competitor's website or other reputable source, we will continue to honor that promise for up to one year after your purchase.
We'll match the price at time of purchase, or refund you the difference if done after the purchase. Either way, we are totally committed to providing you the best online shopping experience.
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Our huge database is regularly cross-checked to keep it current and optimized. At times, our thoroughness makes our data even more accurate than our suppliers! This rigorous process means that you can always order with confidence.


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MENLO PARK, Calif., May 07, 2007 /PRNewswire-FirstCall/ -- Citing issues related to potentially sub-optimal therapeutic dosing of paclitaxel, Conor Medsystems, LLC has concluded that the COSTAR II (CObalt Chromium STent with Antiproliferative for Restenosis) trial, the pivotal study for the CoStar cobalt chromium paclitaxel-eluting coronary stent, failed to meet its primary endpoint. The trial did not identify safety issues, and the overall rates of death, myocardial infarction and stent thrombosis were consistent with those observed in other clinically relevant drug-eluting stent studies.
As a result of these outcomes, the company will terminate ongoing clinical trials with the CoStar(R) stent and will not conclude the submission of its Pre-Market Approval application to the U.S. Food and Drug Administration for the product. Further, Conor Medsystems will discontinue sale of the product through commercial partners in certain countries in Europe, Asia and Latin America where the CoStar stent is already approved, and will work with those partners to facilitate return of product in inventory in customer accounts.
The COSTAR II trial compared the CoStar(R) stent with the Taxus Express(2) paclitaxel drug-eluting stent, and was designed to demonstrate non-inferiority at eight-month follow-up with respect to major adverse cardiac events (MACE) in patients with multi-vessel or single-vessel disease. In this trial, MACE was defined as a composite of clinically driven target vessel revascularization, heart attack (new myocardial infarction) related to the target vessel and cardiac death related to an intervened vessel.
"While we are disappointed in these results, we remain
optimistic about the ability of the novel Conor Medsystems
reservoir platform to provide precise and controlled delivery of a
therapeutic agent based on earlier proof of concept testing and
trials," said Campbell Rogers, M.D., chi


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Briefly: Garmin Mac support; Seagate 1TB
updated 10:25 am EDT, Mon April 2, 2007
Garmin Mac support
In brief: The first Apple Store in Italy, which has opened at the ROMAEST mall, is offering shoppers 5 percent off all goods in the store. Garmin has released WebUpdater for Mac 126.96.36.199 Beta, and Seagate may be preparing to unveil its promised 1TB hard drive by the end of April. Italy's first Apple Store opened with a 5 percent discount to shoppers in the ROMAEST mall, a tactic that is rarely seen with Apple Store openings. New photos have surfaced of the store from setteB.IT, depicting the 5 percent off sale and the long shelves of software, games, accessories, and other Apple-related products. Desktop and portable Macs adorn many table tops, while iPod accessories stand waiting for purchase on others. Supplies are apparently high as customers make purchases without hindrance or "out of stock" notifications.
WebUpdater For Mac 126.96.36.199
Garmin has released a Mac version of its WebUpdater software, an application used to install firmware as well as other updates for Garmin products. Garmin warns, however, that its WebUpdater For Mac 22.214.171.124 Beta is believed to be reliable but has not yet been released for production. The release is available for download to offer Mac users experimental support as the company moves to bring full, tested support to Mac systems.
1TB Seagate drive before May?
Seagate earlier this year promised to release a 1TB hard drive by the second half of 2007, and with three months left before that deadline arrives the company claims it is still on track to deliver the new drive. The company will unveil a 7,200rpm drive with a 3Gbps SATA interface, according to Spanish site Chilehardware, using four platters with eight heads and a 16MB buffer.
The new drive is expected by the end of May, and will join the only 1TB hard drive currently on the market from Hitachi -- the Deskstar 7K1000.


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User talk:Azrael Nightwalker
Hello, Azrael Nightwalker, and welcome to Wikipedia! Thank you for your contributions. I hope you like the place and decide to stay. Here are some pages that you might find helpful:
- The five pillars of Wikipedia
- Tutorial
- How to edit a page
- How to write a great article
- Manual of Style
I hope you enjoy editing here and being a Wikipedian! Please sign your messages on discussion pages using four tildes (~~~~); this will automatically insert your username and the date. If you need help, check out Wikipedia:Questions, ask me on my talk page, or ask your question and then place
{{helpme}} before the question on your talk page. Again, welcome! -- Craigtalbert (talk) 18:43, 24 December 2007 (UTC)
Hay, I just wanted to say thank you for contributing to this article. If you have any questions, just let me know, and I look forward to your future edits. Coffeepusher (talk) 07:07, 9 January 2008 (UTC)
Your edit/summary comment on AT&T Computer Systems[edit]
Hi, your recent summary comment says "SCO didn't bought the USL, they've just licensed Unix". The Novell press release of the time says, "SCO has purchased the UnixWare business from Novell"; that seems like something more than just a licensing arragement. Do you have more info on this, especially citations? It would help in other articles.
I don't think your edit is off base, since the point of that part of the AT&T article is more about AT&T getting out of the UNIX business than where the pieces eventually landed, but I'm not sure your summary comment was accurate. Cheers,--NapoliRoma (talk) 15:49, 16 January 2008 (UTC)


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Working in the United States
|Initial Period of Stay
|Extension of Stay
|1 to 6 months; 6 months is the maximum
|Up to 6 months; maximum total amount of time permitted in B-1 status on any one trip is generally 1 year.
At the port of entry, an immigration official must authorize your admission to the United States, and, if you are eligible for admission, you may be admitted initially for the period necessary to carry out your business activities, up to a maximum period of 1 year. If you who wish to stay beyond the time indicated on the Form I-94 without departing from the United States, you must file Form I-539, Application to Extend/Change Nonimmigrant Status and submit any required supporting documents to USCIS. For more information, see the "Extend my Stay" page.
Family of B-1 Visa Holders
Your spouse and children are not eligible to obtain a dependent visa. Each of your dependents who will be accompanying or following to join you must apply separately for a B-2 visa and must follow the regulations for that visa.
Certain B-1 Activities that Require an Employment Authorization Document
The following types of B-1 business visitors require employment authorization:
- A personal or domestic servant who is accompanying or following to join an employer who seeks admission into, or is already in, the United States in a B, E, F, H, I, J, L, or TN nonimmigrant classification.
- A domestic servant of a U.S. citizen accompanying or following to join his or her U.S. citizen employer who has a permanent home or is stationed in a foreign country, and who is temporarily visiting the United States.
- An employee of a foreign airline engaged in international transportation of passengers freight, whose position with the foreign airline would otherwise entitle the employee to treaty trader nonimmigrant classification (E-1) and who is precluded from such classification solely because the employee is not a national of the country of the airline's nationality or because there is no treaty of commerce and navigation in effect between the United States and the country of the airline's nationality.
Note: All applicants for a B-1 visa or admission as a B-1 business visitor as a personal or domestic servant described above must demonstrate the following:
- You have a residence abroad in which you have no intention of abandoning
- You have at least 1 year of experience as a personal or domestic servant
- You have been employed abroad by your employer for at least 1 year prior to the employer's admission into the United States or if you have been employed abroad by the employer for less than 1year, the employer must show that while abroad, he or she has regularly employed a domestic servant in the same capacity as that intended for your employment
Before you may commence employment in any of the above three activities, you will need to file Form I-765, Application for Employment Authorization. For more information on filing the Form I-765, see the "Work Authorization" page.
More Information
- I-539, Application To Extend/Change Nonimmigrant Status
- I-765, Application for Employment Authorization


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Our Morgan City, LA Hotel's Amenities are the Best Located off Highway 90 and right in the middle of everywhere is the Holiday Inn® Morgan City hotel. Our location means guests are only minutes from local attractions and businesses, and we're within 60 miles of Lafayette, New Orleans and Baton Rouge. Southern hospitality with a Cajun flair welcomes our hotel's guests. Lodging in Morgan City, LA gives you many options for family fun, from picnics at Lake End Park to boutique shopping downtown. Visitors love attending the Louisiana Shrimp & Petroleum Festival, which celebrates the state's heritage with authentic Cajun cooking, Zydeco and Country music performances, a 5K run and more. Ideal for travelers planning meetings or corporate events, our hotel's facility in Morgan City, LA includes a 2,500-sq-ft ballroom, a Business Center and audio/visual support. Business guests also appreciate an easy commute to such companies as Oceaneering International, J Ray McDermott, PHI and Cameron. When it's time for a break from the grind, the Atchafalaya at Idlewild Golf Course is only minutes away. Quality perks like free high-speed, Wi-Fi Internet access and a Fitness Center make lodging in Morgan City a great choice. Our restaurant, Morgan's, is a local favorite, and Legend's, our lounge, is the local hot spot. You'll feel pampered in a room with HDTV, a microwave and a refrigerator. Let us help you make lifetime memories on your next visit!InterContinental
24 hour front desk
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Outdoor pool
Free parking
Pets allowed on request. Charges may apply.
Wheelchair accessible
Room service
Wedding services
Free Wi-Fi
Get Rates
Get Flight + Hotel


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11. Cornely OA et al. Fidaxomicin versus vancomycin for infection with Clostridium difficile in Europe, Canada, and the USA: a double-blind, non-inferiority, randomised controlled trial. Lancet Infect Dis. 2012;12:281-289.
12. Cornely OA et al. Resolution of Clostridium difficile-Associated Diarrhea in Patients With Cancer Treated With Fidaxomicin or Vancomycin. J Clin Onc. 2013;31(19):2493-2499.
13. Mullane KM et al. Efficacy of Fidaxomicin Versus Vancomycin as Therapy for Clostridium difficile Infection in Individuals Taking Concomitant Antibiotics for Other Concurrent Infections. Clin Infect Dis. 2011;53(5):440-447.
14. Mullane KM et al. Renal impairment and clinical outcomes of Clostridium difficile infection in two randomized trials. Am J Nephrol. 2013;38(1):1-11.
15. Loo VG et al. A predominantly clonal multiinstitutional outbreak of Clostridium difficile-associated diarrhea with high morbidity and mortality. N Engl J Med. 2005;353 (23):2442-2449.
16. Campbell R et al. Length of stay and hospital costs among high-risk patients with hospital-origin Clostridium difficile-associated diarrhea. J. Med Econ. 2013;16(3):440-448.
17. Kim JH et al. Clostridium difficile infection in a long-term care facility: hospital-associated illness compared with long-term care-associated illness. Infect Control Hosp Epidemiol. 2011;32(7):656-660.
18. Zilberberg MD et al. Clostridium difficile-associated disease and mortality among the elderly critically ill. Crit Care Med. 2009;37(9):2583-2589.
19. Bauer MP et al. European Society of Clinical Microbiology and Infectious Diseases (ESCMID): treatmen
|SOURCE Astellas Pharma Europe Ltd
Copyright©2012 PR Newswire.
All rights reserved


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UCLA student Rocco Pucillo has won the top prize at the 58th annual Samuel Goldwyn Writing Awards for his script "Inspector Sun and the Curse of the Black Widow."
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Samuel Goldwyn Award Won by Rocco Pucillo
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As The New York Times
The 9/10 President
March 10, 2003
It disappeared so quickly that it is easy to forget the bipartisan patriotism and common purpose that existed in Washington immediately after September 11, 2001. Perhaps the most memorable event from that period was the gathering of members of Congress from both parties on the steps of the Capitol to sing "God Bless America." Another such episode--little-noticed, but actually more remarkable--occurred the following month.
Fair Ball
July 01, 2002
Italy's a wonderful place to honeymoon. Every city has plush hotels with vistas. Every restaurant has extraordinary cuisine. And every television in every bar has soccer. On my just-completed Italian honeymoon, I watched approximately 30 of the 48 first-round matches of the World Cup. My wife and I coordinated our visits to vineyards and duomos so we could catch essential games like Paraguay versus South Africa.


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:O jaw dropping
the green thing that you can see when the turbolasers hit it are very nice to!
Thrawn's Revenge is a large-scale mod for Empire at War: Forces of Corruption. It includes the events of the Galactic Civil War after Palpatine's death at Endor, up until the signing of the Pellaeon-Gavrisom treaty. If you own the Steam version of EaW, you will have to complete extra steps to run the mod after installation. Please see the manual for these steps.
:O jaw dropping
the green thing that you can see when the turbolasers hit it are very nice to!
!!!!!!!!!!!!!! *died of stroke, too much to handle*( last thoughts: this is ******* badass! i hope i hope i hope i get to play it, damn why isn't my heart beating?!? NOOOOOOOOOooooooooooooooo *dead*)
Spaztastic aray of colours.
i love the effects!!!!!
I'd prefer the particle effects of the lasers to be more subdued and washed out. Color is nice...but not at the expense of several million cells in my retina. Also, canonically, they weren't THAT colorful. Maybe you could make two versions and let people exchange them? For the future installments like Ascendancy I mean.
The new Ascendancy model ingame. Design, model and skin by Corey. Still needs an updated light map and spec map but for all intents and purposes it's done.
Remember to vote for us in MotY, you know you want to!


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Being Human
Psychological and Philosophical Perspectives
(If any tax is payable it will be calculated and shown at checkout.)
Preview (read now) Add to my own site Give this ebook to a friend Add to my wishlist Author's page Publisher's page
moreRichard Gross turns his expert eye to the psychology of human nature in a contemplative account encompassing cognition, consciousness, language, time perception, sense of mortality and human society. This book will help you to consider the unique aspects of being human and to understand the biological underpinnings for the intriguing and distinct behaviours and experiences common to human beings.
The book is enhanced throughout by:
- its logical arrangement of topics, with key questions, issues for additional focus and reflection points highlighted throughout
- useful chapter introductions and summaries to provide clarity and insight
- diagrams to help explain difficult concepts
- detailed selection of references and useful sources including works from the fields of psychology, philosophy, religion and literature
This book is essential reading for students of psychology and related disciplines as well as general readers seeking insights into one of the most enduring questions to have faced humankind throughout history. less
Taylor and Francis; January 2013
350 pages; ISBN 9781134657582
Read online, or download in secure EPUB or secure PDF format
Title: Being Human
Author: Richard Gross
350 pages; ISBN 9781134657582
Read online, or download in secure EPUB or secure PDF format
Title: Being Human
Author: Richard Gross
Buy, download and read Being Human (eBook) by Richard Gross today!
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Mindset 2006 US$ 10.99
Feeling Good 2012 US$ 8.99 736 pages
Understanding Homicide 2005 US$ 61.00 368 pages
Emotional Intelligence 2012 US$ 13.99
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Resolution: standard / high
Figure 2.
Fluorescent antibody staining of GAS (red) within the crypts (green) of pediatric tonsils removed due to recurrent GAS tonsillopharyngitis. (A) GAS within in vivo biofilm from a chinchilla. (B and C) GAS within the tonsillar crypts.
Roberts et al. BMC Pediatrics 2012 12:3 doi:10.1186/1471-2431-12-3


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Gig Harbor's new mayor says revitalizing downtown is priority
Gig Harbor's new mayor-elect, Jill Guernsey, has a long history of serving the people of Gig Harbor.
She has lived here for nearly three decades. She served 10 years on the school board, including seven as president. She was a member of the city's Planning Commission from 2005 until 2011, when she was elected to the City Council.
And, on Nov. 5, she was elected the town's 10th mayor, after running unopposed.
Guernsey, 62, and her husband live within walking distance of City Hall, "and if it snows, I can walk a few blocks and catch the bus to my day job in Tacoma," she said.
Her day job, which she's had for 31 years, is as a land use attorney for Pierce County, though she's quick to say that she has "just three years until retirement."
She's also a Small Cities representative on the Tacoma-Pierce County Economic Development Board — which gives her very good insight into what she considers to be two of the most important issues facing Gig Harbor today — land use and economic development.
"We're very fortunate in Gig Harbor to have property owners who want to improve their properties to encourage economic growth," she said.
Guernsey feels strongly that the change in height limits in the downtown business district will be a good thing for the harbor.
"In 2012 we made some very minor changes to the downtown parking regulations and those changes are really almost invisible. I think that people will find the same thing with the 27-foot heights," she said. "There's really not going to be much visible change at all, contrary to some of the things that have been said."
That's typical of Guernsey's way of looking at things.
She doesn't characterize things as problems, but rather as issues with opportunities.
"I believe that communication and perseverance are important and issues are best resolved sooner rather than later," she said. "And you can't let your ego get in the way."
The No. 1 issue for Gig Harbor right now is making downtown as vibrant and healthy as possible, she said.
"We're all very passionate about downtown, but you can love a city to death and we need balance. We have to lift some of the barriers to allow that vibrancy to happen.
"Good things happen when you lift barriers and if we're all working together, we can keep doing the good things that have happened here."
She noted that the trolley that served the town this past summer was a very good thing. "It was a great project that just sort of fell into our lap. It was very successful and we're working out the details to have it become a permanent thing," she said.
Guernsey has several items on her idea list for revitalizing downtown.
As one who loves to travel and sail with her husband on their sailboat, she sees a need to attract more recreational boaters to the harbor "from all over Puget Sound and Canada. We have something very unique here and we need to entice more boaters to come here and spend the night, spend money and then leave," she said.
She noted that the city's 2014 budget includes installing water and power at the city dock and she also wants to see a fuel dock constructed somewhere in the harbor.
She also supports the move that's underfoot in the local arts community to build a regional arts center nearby. "That's another good, quality project and Gig Harbor North would be a very good location for it," she said, adding that many of the ideas presented by the Storefront Studio were also very exciting possibilities.
"I love the idea of having a year-round indoor-outdoor farmers market downtown," she said. "Up in Friday Harbor a group got together and bought an old brickyard building and turned it into a year-round market. It's all done by volunteers and it's very successful."
Guernsey said she was very surprised that she ran unopposed for the mayor's job. "I've always wanted to do it, but I thought that some other council member would want to run. When they all encouraged me, I just decided to go for it," she said.
Come January, she said, she's looking forward to working with the council in her new capacity.
"And I'm also looking forward to working with city staff and our business people," she said, "and building relationships with organizations on the other side of the bridge such as the Port of Tacoma."
Outgoing mayor Chuck Hunter, who's led the city since 2006, said, "I wish Jill the best as she takes over the city. She's very well qualified and I'm sure she's going to be a good mayor."
As for Hunter, come January, he and his wife, Diane, have a trip to Panama on their schedule. "And we've got plenty of stuff here at home to keep us busy. Frankly, I'm looking forward to having my freedom," he said with a smile.
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8520 Highway 1 Unit F-11
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Just wanted to say a big thank you to you and the rest of the Aztec team for making our wedding day perfect and run so smoothly. Lots of our guests commented on how nice the hotel and staff were. Thanks again!
We had an amazing day and we can't thank yourself and Kristina enough for everything you have done for us to make our day so special. Everything was perfect and the whole hotel couldn't have done enough for us and our families. Thank you all so much.
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Integration of molecular, genetic and cytological maps is still a challenge for most plant species. Recent progress in molecular and cytogenetic studies created a basis for developing integrated maps in cucumber (Cucumis sativus L.).
In this study, eleven fosmid clones and three plasmids containing 45S rDNA, the centromeric satellite repeat Type III and the pericentriomeric repeat CsRP1 sequences respectively were hybridized to cucumber metaphase chromosomes to assign their cytological location on chromosome 2. Moreover, an integrated molecular cytogenetic map of cucumber chromosomes 2 was constructed by fluorescence in situ hybridization (FISH) mapping of 11 fosmid clones together with the cucumber centromere-specific Type III sequence on meiotic pachytene chromosomes. The cytogenetic map was fully integrated with genetic linkage map since each fosmid clone was anchored by a genetically mapped simple sequence repeat marker (SSR). The relationship between the genetic and physical distances along chromosome was analyzed.
Recombination was not evenly distributed along the physical length of chromosome 2. Suppression of recombination was found in centromeric and pericentromeric regions. Our results also indicated that the molecular markers composing the linkage map for chromosome 2 provided excellent coverage of the chromosome.
Cucumber (Cucumis sativus L., 2n = 2x = 14) is an economically important vegetable crop in the Cucurbitaceae family. The cucumber genome has been sequenced using a novel combination of traditional Sanger and next-generation Illumina GA sequencing technologies [1]. Illumina GA sequencing technology has significantly improved high throughput sequencing efforts at reasonable cost. However, an intrinsic characteristic of the technology is short read lengths (~50 bp), which prevents their direct application for de novo genomic assembly. Within a total of 72.2-fold genome coverage generated for cucumber genome, Sanger reads provided 3.9-fold coverage and Illumina GA reads provided 68.3-fold coverage [1]. The total length of assembled cucumber genome was 243.5 Mb which is 30% smaller compared to cucumber genome size. Of these, only 72.8% of the assembled sequences were anchored onto the chromosomes using information from high density genetic map previously developed by Ren et al. [2]. However, the genetic map reports only the linear order of markers and the amount of recombination between linked markers. Because linkage map distances are not simply related to physical distances, the linkage map does not provide sufficient detail to support genome assembly. The molecular cytogenetic map incorporating data from both genetic and cytological maps can provide sufficient detail of the physical locations of genetic markers. Such maps can contribute significantly to the assembly of ongoing cucumber genomic sequences by resolving the order of closely linked markers, confirming the physical positions of markers on the linkage groups and evaluating the size of the putative remaining gaps [3,4].
The direct way to generate a cytogenetic map is to localize genetic markers onto chromosomes by fluorescence in situ hybridization. However, most genetic markers (0.5-4.0 kb) are too small to generate consistent and reliable in situ hybridization signals on plant chromosomes [5]. Large insert DNA clones, such as bacterial artificial chromosome (BAC) or yeast artificial chromosomes (YAC) clones, are likely to contain dispersed repetitive sequences that will cause high background signal in FISH [6]. BACs from species such as wheat, with very large genomes do not generate unique locus-specific FISH signals [7]. Small fosmid clones (30-40 kb) likely to contain less dispersed repetitive sequences compared to large insert DNA clones, will be more suitable as DNA probes. A fosmid library was recently constructed for C. sativus inbred line 9930 which was previously used for International Cucumber Genome Project. A high-density polymorphic simple sequence repeat (SSR) genetic map was developed based on whole genome shotgun sequences [2]. In addition, a karyotype showing the position and fluorescence intensity of signals generated by several tandem repeat sequences has been developed for C. sativus inbred 9930 [8]. These accomplishments have created the basis for the integration of molecular, genetic and cytological maps of cucumber. FISH mapping of DNA clones anchored with genetically mapped DNA markers to pachytene bivalents is a very efficient approach to integrate genetic linkage maps with chromosomal maps [9]. Not only do the pachytene chromosomes provide superior mapping resolution compared to somatic metaphase chromosomes, but the euchromatin and heterochromatin features can be visualized on pachytene chromosomes, thereby allowing DNA probes to be mapped to specific euchromatic or heterochromatic regions. To date, FISH-based cytogenetic maps on pachytene chromosomes have been developed in A. thaliana for chromosome 4 [10], maize chromosome 9 [11,12], potato chromosome 6 [13,14], Brassica oleracea chromosome 6 [15], rice chromosomes 5 and 10 [4,16], tomato chromosomes 1, 2 and 6 [17-19], soybean chromosome 19 [20], cotton chromosomes 12A and 12D [21] and for all the Sorghum chromosomes [22,23]. We previously reported integrated cytogenetic maps for cucumber chromosomes 6 and 7 [24]. Here, we report an integrated cytogenetic map for cucumber chromosome 2 using similar methods as described previously [24].
The distribution of 45S rDNA, Type III and CsRP1 sequences on cucumber metaphase chromosome 2
Our previous study demonstrated that the satellite repeat sequence Type III located at cytologically defined cucumber centromeres, and the Type III signals on chromosome 2 were the weakest among the seven chromosome pairs [8]. In this study, we found that minor Type III signals also occurred at the interstitial regions of chromosome pairs 2 (arrows in Figure 1a, b) and 4 (arrowheads in Figure 1a, b) which were identified by FISH mapping of the 45S rDNA probe simultaneously on optimal chromosome preparations (Figure 1c). The minor signals on chromosome 4 (arrowheads in Figure 1b) were much weaker than those on chromosome 2 (arrows in Figure 1b). Interestingly, an unusual distribution of the 45S rDNA sequence was found. The signals of 45S rDNA, which are usually associated with a secondary constriction, occupied the exclusive constriction region. The major and minor signals of Type III sequence, which located at the primary constriction regions on other cucumber chromosomes, flanked the constriction on chromosome pairs 2 (arrows in Figure 1c) and 4 (arrowheads in Figure 1c). Moreover, the minor Type III signals on the two chromosome pairs colocalized with strong signals of the CsRP1 sequence (Figure 1d-f), which is a pericentromeric heterochromatin specific repeat [25]. The stronger signals of CsRP1 were close the minor Type III signals on chromosome 2 (arrows in Figure 1d-f).
Figure 1. Distribution of 45S rDNA, Type III and CsRP1 sequences on cucumber metaphase chromosomes 2 and 4. a-b Minor Type III (green) signals in the interstitial regions of chromosome pairs 2 (arrows) and 4 (arrowheads). c The 45S rDNA (red) signals occupied the exclusive constriction region and the signals of major and minor Type III satellite repeat flanked the constriction on chromosome pairs 2 (arrows) and 4 (arrowheads). d-f The minor Type III (green) signals were colocalized with strong signals of the CsRP1 (red) on chromosome pairs 2 (arrows) and 4 (arrowheads). Bars, 5 μm
The construction of molecular cytogenetic map of chromosome 2
To construct a cytogenetic map with a high resolution, an initial set of 16 SSR markers from linkage group 2 [2] was selected to screen a fosmid library developed from the cucumber inbred line 9930. The SSR markers were distributed at an average distance of ~6 cM along the linkage group from 0.0 cM (bin 1) to 100.2 cM (bin 113). Only 11 fosmid clones were selected for FISH mapping which produced little or no background signal when hybridized to cucumber chromosomes without the aid of C0t-1 DNA for blocking (Table 1).
Table 1. Genetic and physical locations of SSR markers and their corresponding fosmid clones
We first determined the physical order of adjacent fosmid clones based on the genetic positions of their corresponding SSR markers by dual-color FISH on somatic metaphase chromosomes (Figure 2a). On the basis of these results, multi-fosmid FISH probe cocktails were developed and hybridized to the pachytene chromosomes together with the cucumber centromere-specific DNA probe Type III (Figure 2b). The relative position of all probes can be clearly distinguished on spreads of pachytene bivalents. Three computationally straightened chromosomes 2 from three independent cells were shown (Figure 2c). The left chromosome is straightened from the image shown in Figure 2b. The DAPI-stained pachytene chromosomes in Figure 2c were converted into a black-white image to show heterochromatin distribution (Figure 2d). Pachytene chromosome 2 showed a distinct heterochromatin and euchromatin distribution pattern based on DAPI staining. The heterochromatic region spanned ~18% of the length of the chromosome. Most of the heterochromatin was confined to the pericentromeric region. Small heterochromatic domains were observed at both ends of the chromosome (Figure 2d). Using the FISH signals from Type III repeat as a reference, the centromere for chromosome 2 was placed between the fosmids 2-6 and 2-7.
Figure 2. FISH of 11 fosmids on cucumber somatic and pachytene chromosomes. a FISH of 11 fosmids on somatic chromosomes. Adjacent probes were labeled with different fluorochromes and hybridized together to test the order and position. b Cucumber chromosomes at the pachytene stage were probed by a set of eleven fosmid clones together with the Type III satellite repeat. c Three straightened cucumber pachytene chromosome 2. The left chromosome is straightened from the image shown in Figure 2b. d The chromosomes in Figure 2c were converted into black-white image. Distinct heterochromatin is visible at the centromeric region and at the distal ends of both chromosome arms. Bars, 5 μm
The order of individual fosmids along the chromosome was generally colinear with the order of the corresponding SSR markers along the linkage map except for 2-10 (94.3 cM) and 2-11 (97.7 cM). The FISH signals derived from fosmid clones 2-10 and 2-11 were located adjacently on pachytene chromosomes but 2-10 was located closer to the telomere (Figure 2c). We examined information from the genome sequence in the region that showed conflict between the genetic map and our FISH result. We found that genome sequence information supported our FISH result (unpublished data). The conflict may be either due to a result of chromosomal rearrangement that occurred between the sequenced genotype 9930 and the genotypes used to create the mapping population or these markers may have been placed incorrectly on the genetic map.
To assess the rate of recombination, measurements of fosmid positions along the six straightened chromosomes 2 were taken and transformed into fractional lengths (FL) using a methodology reported by Cheng et al. [4]. The relationship between the genetic and physical distances along chromosome 2 is summarized in Table 1 and Figure 3. In general, recombination is more or less evenly distributed along the physical length of chromosome 2 (Figure 3). However, a significant disproportion between genetic and physical distances was found in the centromeric region. SSR03758 (57.5 cM) and SSR23732 (61.5 cM) are the closest SSR markers flanking the centromere. These two markers are separated genetically by only 4 cM but physically by 30.9 FL.
Figure 3. Comparison of the genetic linkage map and pachytene FISH-based cytogenetic map for cucumber chromosome 2. The linkage map was drawn according to Ren et al. [2]. The position of each fosmid on the pachytene chromosome is calculated as the fractional length using a methodology reported by Cheng et al. [4]. FL (fraction length) = (S/T) × 100.2, where S = distance (μm) from the FISH site to the end of the long arm, T = total length of the chromosome (μm), and 100.2 is the length (in cM) of the linkage group 2
One of the most important criteria to judge the quality of a genetic linkage map is its physical coverage of the corresponding chromosome. The FISH signals for two fosmids (2-1 and 2-10) were very near on the opposing ends of the chromosome. These results indicate that the molecular markers composing the linkage map for chromosome 2 provide excellent coverage of the chromosome.
Centromere position for cucumber chromosome 2
The centromere is the most characteristic landmark of monocentric chromosomes in higher eukaryotic species and appears cytologically as a distinct primary constriction on condensed metaphase chromosomes. Satellite DNA and retrotransposons are the most abundant DNA elements found in plant centromere regions [26]. Due to large arrays of highly homogenized satellite repeats, elucidating the assembly and structure of chromatin at centromeres is extremely difficult. Thus, the centromeres have been left as gap in the sequence maps in most model eukaryotes. rDNA sequences and several types of satellite sequences primarily located in the centromeric and telomeric regions comprised the majority of unassembled reads in cucumber genome shotgun sequences [1]. FISH is a powerful technique to delineate the structure and DNA composition of such genomic regions [9].
Results of the cytogenetic studies on cucumber chromosome 2 have been inconsistent in previous karyotypes [8,25,27,28], due to different positions assumed for centromere of chromosome 2. In our study, FISH results showed an unusual distribution of the 45S rDNA sequence and the existence of minor centromere-specific satellite loci on chromosome 2, which may have led to confusion about the actual centromere position on this chromosome. The centromere of chromosome 2 was previously assigned to positions which correspond to the chromosomal regions of either the 45S rDNA, the major or the minor Type III repeat signals [8,25,28]. CENH3 is a good marker for assaying centromere activity since it is found only in functional centromeres in eukaryotes [29-35]. The preparation of anti-CENH3 antibody in cucumber would be used to prove the location of functional centromere on cucumber chromosome 2.
Relationship between genetic and physical distance for cucumber chromosome 2
Our fosmid map of cucumber chromosome 2 revealed a significant disproportion between genetic and physical distances in the centromeric region. Indeed, reduction of recombination around the centromere is a common feature, especially for grasses with large genomes and high repetitive DNA content, such as wheat, barley, and sorghum. Crossing-over in the (peri-)centromeric regions, which may account for as much as 50% of the chromosome length, is essentially suppressed [23,36-40]. In genomes with smaller chromosomes, such as rice and Arabidopsis, cross-over suppression at the centromere is restricted within a relatively small region [4,41,42]. Like rice and Arabidopsis, the region of recombination suppression on chromosome 2 is also restricted to a relatively small region corresponding to ~30% of the chromosome length. Since the region of recombination suppression correlates directly with sizes of centromeric heterochromatin regions [4], the regions of recombination reduction from other cucumber chromosomes should be smaller than the region on chromosome 2 because the proportion of centromeric heterochromatic regions on chromosome 2 is the highest one of all cucumber chromosomes [25]. This has been confirmed by our previous study which demonstrated that an obvious recombination reduction was not detected for cucumber chromosome 6. Also the region of suppressed recombination of chromosome 7 was smaller than that of chromosome 2 [24].
The integrated genetic and cytogenetic maps can serve as a template to facilitate sequence assembly because the maps provided information on the distribution of heterochromatin, euchromatin, centromeres and markers across chromosomes [4,43]. In addition, the cytogenetic maps can be used to determine if the linkage gaps represent recombination hot spots or large chromosomal segments. The assembled cucumber sequences were anchored onto the chromosomes using a high-density genetic map information developed by Ren et al. [2]. Although the genetic linkage map contains more markers per centimorgan than any other cucumber genetic map, it still contains large gaps. Integrated molecular cytogenetic maps can indicate whether these gaps are associated with recombination hot spots and/or represent a large chromosomal segment. Such information will be valuable for designing corresponding strategies to eventually close these gaps.
Using a combination of molecular, genetic and cytological approaches to the analysis of cucumber genome, we built an integrated map of cucumber chromosome 2, to provide also an explanation for previous different localization of the centromere of this chromosome and find that the meiotic recombination frequency is reduced around the cucumber centromeres. With the establishment of cytogenetic maps of other cucumber chromosomes, this integrated set of information will not only provide a framework for cucumber genome assembly but also provide a solid foundation for cucumber genetic and genomic research such as map-based gene isolation, comparative genomics and evolutionary studies.
Plant materials and chromosome preparation
C. sativus 'Chinese long' inbred line 9930 was used for cytological studies. Root tips were harvested from germinated seeds, pretreated in 0.002M 8-hydroxyquinoline at room temperature for 2 h to accumulate metaphase cells, and fixed in methanol:glacial acetic acid (3:1). Root tips were macerated in 2% cellulase Onozuka R-10 (Yakult Pharmaceutical, Tokyo) and 1% pectolyase Y-23 (ICN) at 37°C for 2 h and squashes were made in the same fixative. Young panicles were harvested and fixed in 3:1 (100% ethanol:glacial acetic acid) Carnoy's solution. The procedure for meiotic chromosome preparation was largely the same as that used for preparing mitotic chromosomes from root tips with the following modification: anthers were digested in the enzyme mixture for 4.5 h at 37°C. The digested anthers were macerated on glass slides in 50% acetic acid solution with fine-pointed forceps and then flame-dried.
Fluorescence in situ hybridization (FISH)
All fosmid clones were provided by the Beijing Genomics Institute, Beijing, China. The fosmid library was constructed from genomic DNA of inbred line 9930 which was also used for whole genome sequencing. SSR markers spaced ~6 cM apart across linkage groups 2 [2] were used to select fosmids for FISH. Fosmid DNA was isolated using QIAGEN plasmid midi kit and further purified by Plant DNeasy spin columns (QIAGEN). The 45S rDNA, Type III and CsRP1 clones of cucumber [8] were used. FISH was performed according to published protocols [44]. DNA probes were labeled with digoxigenin-dUTP or biotin-dUTP via nick translation and detected with antidigoxigenin antibody coupled with Rhodamine (Roche) or avidin-conjugated with FITC (Vector Laboratories), respectively. Chromosomes were counterstained by 4,6-diamidino-2-phenylindole (DAPI) in a VectaShield antifade solution (Vector Laboratories). Images were captured digitally using a CCD camera (QIMAGING, RETIGA-SRV, FAST 1394) attached to an Olympus BX61 epifluorescence microscope. Gray-scale images were captured for each color channel and then merged. Chromosome straightening was performed using the 'straighten-curved-objects' plug-in of Image J [45], and final image optimization was performed using Adobe Photoshop (Adobe Systems).
Authors' contributions
YH, SH and WJ designed research. YH and ZZ performed research. YH and WJ analyzed data and wrote the paper. All authors read and approved the final manuscript.
The authors are grateful to Dr. Bala Pudota (University of Wisconsin_Madison) for valuable comments on the manuscript and Ms. H. Miao (Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences) for supplying young panicles of C. sativus inbred line 9930. This research was supported by the financial support from China Postdoctoral Science Foundation to Yonghua Han (20090460049), by the the National Science Foundation of China (31025018) to Weiwei Jin and by the Ministry of Agriculture ("948" program 2008-Z42) to Sanwen Huang.
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Koo DH, Hur Y, Jin DC, Bang JW: Karyotype analysis of a Korean cucumber cultivar (Cucumis sativus L. cv. Winter Long) using C-banding and bicolor fluorescence in situ hybridization.
Mol Cells 2002, 13:413-418. PubMed Abstract
Henikoff S, Ahmad K, Malik HS: The centromere paradox: Stable inheritance with rapidly evolving DNA.
Science 2001, 293:1098-1102. PubMed Abstract | Publisher Full Text
Talbert PB, Masuelli R, Tyagi AP, Comai L, Henikoff S: Centromeric localization and adaptive evolution of an Arabidopsis histone H3 variant.
Plant Cell 2002, 14:1053-1066. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Zhong CX, Marshall JB, Topp C, Mroczek R, Kato A, Nagaki K, Birchler JA, Jiang JM, Dawe RK: Centromeric retroelements and satellites interact with maize kinetochore protein CENH3.
Plant Cell 2002, 14:2825-2836. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Nagaki K, Cheng Z, Ouyang S, Talbert PB, Kim M, Jones KM, Henikoff S, Buell CR, Jiang J: Sequencing of a rice centromere uncovers active genes.
Nat Genet 2004, 36:138-145. PubMed Abstract | Publisher Full Text
Nagaki K, Kashihara K, Murata M: A centromeric DNA sequence colocalized with a centromere-specific histone H3 in tobacco.
Chromosoma 2009, 118:249-257. PubMed Abstract | Publisher Full Text
Jin WW, Melo JR, Nagaki K, Talbert PB, Henikoff S, Dawe RK, Jiang JM: Maize centromeres: organization and functional adaptation in the genetic background of oat.
Plant Cell 2004, 16:571-81. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Han YH, Wang GX, Liu Z, Liu JH, Yue W, Song RT, Zhang XY, Jin WW: Divergence in centromere structure distinguishes related genomes in Coix lacryma-jobi and its wild relative.
Chromosoma 2010, 119:89-98. PubMed Abstract | Publisher Full Text
Werner JE, Endo TR, Gill BS: Toward a cytogenetically based physical map of the wheat genome.
Proc Natl Acad Sci USA 1992, 89:11307-11311. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Gill KS, Gill BS, Endo TR: A chromosome region-specific mapping strategy reveals gene-rich telomeric ends in wheat.
Chromosoma 1993, 102:374-381. Publisher Full Text
Delaney DE, Nasuda S, Endo TR, Gill BS, Hulbert SH: Cytogenetically based physical maps of the group-2 chromosomes of wheat.
Delaney DE, Nasuda S, Endo TR, Gill BS, Hulbert SH: Cytogenetically based physical maps of the group-3 chromosomes of wheat.
Künzel G, Korzun L, Meister A: Cytologically integrated physical restriction fragment length polymorphism maps for the barley genome based on translocation breakpoints.
Genetics 2000, 154:397-412. PubMed Abstract | PubMed Central Full Text
Mayer K, Schüller C, Wambutt R, Murphy G, Volckaert G, et al.: Sequence and analysis of chromosome 4 of the plant Arabidopsis thaliana.
Nature 1999, 402:769-777. PubMed Abstract | Publisher Full Text
Wu J, Mizuno H, Hayashi-Tsugane M, Ito Y, Chiden Y, Fujisawa M, Katagiri S, Saji S, Yoshiki S, Karasawa W, Yoshihara R, Hayashi A, Kobayashi H, Ito K, Hamada M, Okamoto M, Ikeno M, Ichikawa Y, Katayose Y, Yano M, Matsumoto T, Sasaki T: Physical maps and recombination frequency of six rice chromosomes.
Plant J 2003, 36:720-730. PubMed Abstract | Publisher Full Text
Kulikova O, Gualtieri G, Geurts R, Kim DJ, Cook D, Huguet T, de Jong JH, Fransz PF, Bisseling T: Integration of the FISH pachytene and genetic maps of Medicago truncatula.
Plant J 2001, 27:49-58. PubMed Abstract | Publisher Full Text
Jiang JM, Gill BS, Wang GL, Ronald PC, Ward DC: Metaphase and interphase fluorescence in situ hybridization mapping of the rice genome with bacterial artificial chromosomes.
Proc Natl Acad Sci USA 1995, 92:4487-4491. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Kocsis E, Trus BL, Steer CJ, Bisher ME, Steven AC: Image averaging of flexible fibrous macromolecules: the clathrin triskelion has an elastic proximal segment.
J Struct Biol 1991, 107:6-14. PubMed Abstract | Publisher Full Text


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GM Announces Ugly, 'Semi-Ruggedized' Hummer Laptop
In GM's endless search to capitalize on the popularity of the Hummer brand, they decided it would be a good idea to introduce a Hummer laptop. As one might expect, the laptop is big, ugly and probably consumes more power than any other laptop. This overpriced machine is a result of a 3-year exclusive deal between Hummer and Itronix and the laptop is being billed as 'semi-ruggedized' (sort of like semi-off-road). What that means is that it can withstand a drop from 30 inches onto two layers of plywood over concrete. (Who holds their laptop 30 inches above plywood?).
The only 'semi-cool', 'semi-unique' feature about this new product is that it has built in GPS.
The laptop will come in black and silver with yellow, red or pewter-gray trim and will start at $2,988.
Christen Costa
Grew up back East, got sick of the cold and headed West. Since I was small I have been pushing buttons - both electronic and human. With an insatiable need for tech I thought "why not start a blog focusing on technology, and use my dislikes and likes to post on gadgets."


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While Democratic fundraiser Norman Hsu was arrested last week, blogger Philip "Flip" Pidot was searching for data and crunching numbers to document that the political contributions made by Hsu's network totaled nearly $1.6 million, substantially more than news organizations previously reported.
Posted at his "Suitably Flip" site (http://suitablyflip.blogs.com), Mr. Pidot's blogging reflects the 31-year-old's various interests in economics, technology and politics. A financial analyst with a master's degree in business administration from the University of Virginia, Mr. Pidot was a Republican candidate for the New York state Senate last year, winning 21 percent of the vote in a race against an incumbent Democrat in a liberal district on Manhattan's East Side.
The following questions are excerpts of a recent e-mail interview with Mr. Pidot:
Question: How does a University of Virginia MBA end up blogging about politics?
Answer: If Thomas Jefferson were with us today, I think we'd certainly find him doing some political blogging on the side (think of the time savings if King George was able to read the Declaration in a feed reader).
Before business school, I worked as a corporate fraud investigator. I think part of the appeal of blogging is the opportunity to indulge that sleuthing urge. My primary interests being finance and politics (and the intersection of the two), that's generally what I'm blogging about.
Q: Does blogging help pay your bills? Or is it just a hobby for you?
A: I think I generated about 80 cents when I briefly experimented with Google AdSense a couple of years ago, but since I never received it, I think technically I get to maintain my amateur status.
Q: You did database research, spreadsheets and charts on the Norman Hsu fundraising scandal. Are those kinds of business-school skills helpful in blogging?
A: I think research skills in general are valuable blogging assets, which is probably why we see a lot of lawyers and professors (and law professors) among the most influential bloggers. Being comfortable seeking out, harvesting, analyzing and commenting on data (and doing it fast) is an advantage, and business school is definitely a good training ground for that, but probably no more so than law school, journalism school, etc.
Q: There's been a lot of discussion about why the liberal blogosphere is bigger than the conservative side. If graduate-school skills are so valuable in blogging, do you think the left's online advantage is partly a reflection of the overall liberal dominance of academia?
A: Possibly, though I think there are larger factors affecting the evolution of the political Web. The blogosphere (and the Internet generally), as a free market for ideas, will tend to favor dissenters, since — by definition — they're the ones less well represented by an establishment, whether that establishment is the government or the media. Fueling the growth of the lefty blogosphere is the left's unifying, pathological hatred of our president and everything he does. Fueling the growth on the right is a frustration with the time-honored liberal bias in the traditional media.
Lefty blogging also tends to be more concentrated among a few extremely influential sites, while the conversation on the right tends be more distributed. A couple of years ago, I ran a Herfindahl Index for two blog-hemispheres and came up with a value of 0.31 for the left and 0.15 for the right. The index is typically used to measure an industry's market share concentration, but I think it's equally instructive here. Because influence is more concentrated among fewer voices on the left, the messaging is more focused and consistent. But I'd argue that that's at best a temporary advantage. Like any market, a free market for ideas functions best with vigorous competition among a large number of participants.
Q: If you had to bet $100 on the outcome, who do you think will be the Republican and Democratic presidential nominees in 2008? And, if you had another $100 on the line, who would win that matchup?
A: Mitt Romney over Hillary Clinton.


text: http://www.linuxquestions.org – Linux Educacional is a Brazilian version of Linux, made for digital inclusion in schools. I'm in a project to instruct the children on games development, for this we're using RPG Maker XP, running in Wine.The program works great, in first view the only problem is the windows bar, because it crash when clicked. But now I discovered another problem. (HowTos)


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Saving Private Ryan (2 Disc Academy Awards)DVD
Internationally acclaimed by critics and audiences alike, Steven Spielberg's Saving Private Ryan is an unforgettable film achievement that has had profound and lasting impact throughout the world. Winner of five Academy Awards including Best Director, the film also captured Oscars for Cinematography, Film Editing, Sound and Sound Effects Editing. More than 70 critics (including those at Time magazine, USA Today, The New York Times and Entertainment Weekly) and critics' groups in New York, Chicago, Dallas-Ft. Worth and Great Britain named the film Best Picture of the Year, while the Los Angeles, Toronto and Broadcast Film Critics honoured it with both Best Picture and Best Director awards. In addition, Spielberg received his third Directors Guild of America Award, the American Legion "The Spirit of Normandy" Award, a USO Merit Award from the USO of Metropolitan Washington, as well as the highest civilian public service award from the Department of the Army. Selected for more than 160 Top Ten lists, Saving Private Ryan's other honours include Golden Globes for Best Picture (Drama) and Best Director, the Producers Guild of America Award and ten nominations from the British Academy Film Awards. Saving Private Ryan was the top-grossing motion picture of 1998. Seen through the eyes of a squad of American soldiers, the story begins with World War II's historic D-Day invasion, then moves beyond the beach as the men embark on a dangerous special mission. Captain John Miller (Tom Hanks) must take his men behind enemy lines to find Private James Ryan, whose three brothers have been killed in combat. Faced with impossible odds, the men question their orders. Why are eight men risking their lives to save just one? Surround by the brutal realities of war, each man searches for his own answer - and the strength to triumph over an uncertain future with honour, decency and courage.
Product Details
|Tom Hanks, Matt Damon, Tom Sizemore, Edward Burns
|Steven Spielberg
|2 Discs
|Running Time:
|163 Minutes
|Region 4 DVD - Suitable for Australia and New Zealand
|Dutch, Swedish, Turkish, Hungarian, English, Greek, Icelandic, Romanian, Finnish, Croatian, Serbian, Norwegian, Arabic, Portuguese, Slovenian, Hebrew, Polish, Bulgarian, Danish, Czech
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"Art making is a form of alchemy, in a way, because you are trying…to make gold from nothing. When it works very well is when you manage to turn the ordinary into the extraordinary."
"Excerpt (battle track)" (2003)
"There are different types of work that have informed mine, in terms of how it's made and what it may be quoting. But if you think of an abstractionist who really tried to deal with the social through abstraction, it's Kandinsky."
-Julie Mehretu


text:
Biltmore Membership GolfGolf Membership 2015 - Experience the restored Donald Ross Classic
Annual Membership Rates
Coral Gables Residents
(Pricing Before Taxes)
Singles Walking
Payment in full$3,720
Payment in full$3,720
12-month financing$341
Couples Walking
Payment in full$5,580
Payment in full$5,580
12-month financing$512
Junior (17 years old & under) Walking
Payment in full$900
Singles Riding
Payment in full$5,520
Payment in full$5,520
12-month financing$506
Couples Riding
Payment in full$8,280
Payment in full$8,280
12-month financing$759
Non-Gables Residents
(Pricing Before Taxes)
Singles Walking
Payment in full$4,620
Payment in full$4,620
12-month financing$424
Couples Walking
Payment in full$6,930
Payment in full$6,930
12-month financing$636
Junior (17 years old & under) Walking
Payment in full$1,860
Singles Riding
Payment in full$6,420
Payment in full$6,420
12-month financing$589
Couples Riding
Payment in full$9,630
Payment in full$9,630
12-month financing$883
Membership Amenities
Unlimited Greens Fees for Walkers for 12 months
Unlimited Greens and Cart Fees for Riders for 12 months
Unlimited Practice Balls for 12 months
Seven (7) days in advance starting time reservations
USGA Golf Handicap
20% discount off full retail pricing in The Biltmore Shops, Spa and Restaurants
Tournaments and Events
Complimentary Valet Parking at the lower level Front Entrance to Hotel
The Executive Golfer Collection Partnership. Access 109 golf courses throughout the United States. Learn more
ClubCorp The World Leader in Private Clubs - Access to ClubCorp's 160 golf and country clubs, business clubs, sports clubs and alumni clubs in 25 states, the District of Columbia and two foreign countries. Learn more
*Inquire about Junior Golf Memberships for Children under 17. All Fees are subject to Florida Sales Tax.
Additional Options (AnNual Fees Listed)
Single Bag Storage Fee$300
Couples Bag Storage Fee$450
Additional Golf Bag Storage$150/bag
Questions? Contact Us
Do you have some golf membership questions? Email us at firstname.lastname@example.com or call us at (305) 913-3230


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The study included more than 900 participants and provided estimates of the distributions of dioxin concentrations in blood, soil and dust in the Midland-Saginaw area as well as a reference area for comparison 100 miles to the south. EPA's review found that the UMDES was well-suited to identify patterns of serum dioxin, furan and PCB levels among adults. Among the study's other findings: people living the Midland-Saginaw area have higher blood dioxin levels than those in the reference area and national averages, and that properties in Midland-Saginaw tend to have higher soil dioxin levels than in the reference area. As has been found in other studies, it also found that higher blood dioxin levels were associated with demographic factors such as increased age, dietary choices and being overweight.
Representatives from EPA's Office of Research and Development and Office of Solid Waste and Emergency Response will hold a public meeting in the Midland-Saginaw area in late October to discuss the UMDES review. More information will be announced soon.
|Contact: Mick Hans
U.S. Environmental Protection Agency


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The next step in the butterfly garden will be to install the fence between the garden and road. We'll be doing this on Saturday, October 31st starting at 8:30 a.m. Any help would be appreciated.
Work Days
We are adding a Green Power Hour work session on the second Monday of the month, from 5-6 p.m.
Regularly scheduled work days will be the third Saturday of the month, 9-noon. We generally have enough tools for everyone, but bring your favorite work gloves, a hat and water bottle!
Please circle the dates on your calendar and plan to join in the garden work day fun. If you have questions...
Contact Ed McNally at 828-333-3883 with questions.
Design Plans
Now that the butterfly garden has been planted, we're working on a design for the BIRD GARDEN. This garden will be located on the back side of the library. Access the area by going through the interior doors on either side of the large windows, or just sit inside and watch as the landscape changes.
Interested in helping? Give Ed a call at 828-333-3883 to volunteer
Below are some of the native grasses we used in the circle garden.... Check them out!
You'll notice some of the same plants that were used in the entry gardens will be used in the circle design. Some basic principles of design are repetition and continuity. So you'll find as we design the library grounds that we repeat plants and color to help pull all the gardens together.
Have design questions, call Ed 828-333-3883 or Susan 828-656-2729.
Designs for other gardens are being worked on as well. Interested in getting involved?
We've started on the butterfly garden and will focus our planting this spring in that area. The paths have been established and some of the larger plants put into the ground. There is a lot of interest in the monarch project and we will plant to encourage monarch butterflies to feed and reproduce in our gardens. Call Bobbi at 828-689-5100 if you want to help with this project.
Michael Tousey is interested in designing and installing the woodland garden, but first needs to spend some serious time removing the invasives in that area. If you are interested in working on this project with him, give him a call at 828-689-5100.
Ed McNally and Michael Redfox are working on the outcrop area and the far edge of the the parking area, across from the entry gardens. Take a look at the work that has been started. Its looking great!! Give Ed a call at 828-333-3883 and let him know what you think.
Below are some of the interesting native plants we used in the entry garden. Check them out and see if they might fit into your landscape as well!
Comments or questions? call Ed at 828-333-3883.
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Views: 47
Homes For Haiti Benefit Dinner
Saturday, February 26, 2011 @ 7:00 p.m.
Christ Church Riverdale (Episcopal)
5030 Henry Hudson Parkway
Bronx, NY 10471
What Is The Event About?
An evening of Haitian cuisine and music to raise funds for a home building workshop in Port-au-Prince.
Tickets: $25.00 for adults. Free for children 12 and under. Checks should be made payable to Kleiworks International.
Guest Speaker: The Rev. Nathanael Saint-Pierre, Priest in Charge of the Haitian Congregation of the Good Samaritan, Bronx, NY.
Our work is a collaboration with Kleiwerks International, a non-profit, sustainable building organization, and the Partnership Program of the Episcopal Diocese of Haiti.
12 students from the Partnership Program will participate in a home building workshop through Kleiwerks. They will use their acquired skills to help build earthquake resistant homes in their local communities.
For More Information: Email Bryan Hiott: firstname.lastname@example.com
© 2015 Created by Arikia Millikan. Powered by


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- 4 total posts
- 4 questions
Questions from December2013
My drooping fluctuates. What can surgeons can do to minimize over or under correction? (photo)
Hooded brows corrected w/ blepheroplasty? My drooping fluctuates. I literally hold my eyes open sometimes so i dont look funny. Tested for myasthenia & thyroid - negative.... READ MORE
I would Like to improve my side profile - have considered a nose Job And Not sure how/if it can be corrected? (photo)
My eyes turn upward, I have a large nose, and high set ears. I tend to look down and im not sure if that is an anatomical issue or just a bad habit or what. Gods cruel joke to... READ MORE
What's the best option for my resting face? Cheek augmentation, rhinoplasty, lift lower eyelids and improve shape? Lips? (Photo)
I work in customerservice. Always told I look stoic/tired/weird. always told to smile/people assume something is wrong. My face is a bit asymmetric. I think some sort cheek... READ MORE
Is there something that can be done to prevent squinting without affecting the ability to close the eye?
I have an involuntary habit of squinting without realizing. I have a slight ptosis in one eye but sometimes it looks like I have a more severe ptosis in both eyes. Upon closer... READ MORE


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Wireless Connectivity Issues Resolved - 9/23
Today's issues with the "WSU-Secure" wireless connection have been
resolved. If you are still experiencing issues with wireless
connectivity, please contact the CaTS Help Desk at (937) 775-4827 for
further assistance.
WSU-Secure Wireless Unavailable
Currently, the "WSU-Secure" wireless connection is unavailable. CaTS is
aware of the situation and is actively working with the appropriate
vendor to resolve the issue. In the meantime, wireless connectivity is
still available by using the "WSU_EZ_Connect" connection.
If you have any questions, please contact the CaTS Help Desk at
(937) 775-4827.
Office 365 Password Change Now Available
The password change feature for Office 365 accounts is now available.
If you have questions, please call the CaTS Help Desk at (937) 775-4287 or email@example.com.
Office 365 Password Change Unavailable
Currently, the password change feature for user's Office 365 accounts is unavailable.
If you have questions, please contact the Help Desk at (937) 775-4827 or email@example.com.
Missing Links in WINGS Beta
Currently, some users are missing bookmarks they have previously added to the 'My Links' section in WINGS beta.
If you have any questions, please contact the CaTS Help Desk at (937) 775-4827, or toll free at 1-888-775-4827.
Featured Services
Wright State App
The Wright State App helps you stay connected to the university like never before. Access critical information about your courses, accounts, and other information directly from your phone or tablet.
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Learn more about IT security and how to protect both your personal information and the university's sensitive data.
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Atomic Learning offers video tutorials on current and popular software titles, including Microsoft Office and products from Adobe. Free for all faculty, staff, and students!


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Hansbrough, Beasley, Love headline Wooden finalists
Updated: April 1, 2008, 1:12 PM ETAssociated Press
LOS ANGELES -- D.J. Augustin of Texas, Michael Beasley of Kansas State, Stephen Curry of Davidson, Tyler Hansbrough of North Carolina and UCLA's Kevin Love are finalists for the John R. Wooden Award, given to college basketball's top player.
The award will be presented April 11 in Los Angeles.
Those five were named to the Wooden All-America team announced Tuesday. Joining them are Memphis teammates Derrick Rose and Chris Douglas-Roberts, Brandon Rush of Kansas, Luke Harangody ...
Read full story
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- EcuadorAgricultureFunding via Cooperativa de Ahorro y Credito Mujeres Unidas (CACMU)
Maria BelenEcuadorHealthFunding via Fundacion Alternativa
Marìa TransitoEcuadorHealthFunding via VisionFund Ecuador
Estrella sells glasses and doing eye exams in Guayaquil. After working at an optometrist's office for some time Estrella saved enough money to start her own business. She had to make a $480 investment in the glasses kit that she uses to take people's measurements. After she takes the measurements and her clients chose the frames she sends the glasses in to get the correct lens put in. She mostly sells in her community, but also travels to colleges, churches and hospitals selling her products. She is very popular because there are not any optometrists that will go to their clients, especially in these marginalized communities.
She is currently separated from her husband and has the responsibility of providing for herself and her 15 year old child while he finished high school. Her dream is to finish her studies at the university so she can be a certified optometrist. She eventually wants to open her own office. She will invest the money in new frames and a second display case.
Mifex offers its clients microinsurance and access to business training and educational programs. For more information about microfinance, Ecuador, and our services, please visit www.mifex.org.
About Ecuador
89View loans »
US Dollars
Success!! The loan was 100% repaid
- Repayment Term
- 10 months (Additional Information)
- Repayment Schedule
- Monthly
- Disbursed:
- Nov 27, 2006
- Listed
- Nov 13, 2006
- Currency Exchange Loss:
- Ended:
- Aug 9, 2007


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- Musician's Friend Best Selection, Price & Service. Guaranteed.
Call our Gear Heads
at: 800-449-9128
Call our Gear Heads
at: 800-449-9128
An excellent collection of 34 songs, especially useful in teaching students. This collection is a good alternative or companion to the standard Baroque Italian songs and arias. Now with recorded accompaniments by pianist Laura Ward.
Our product catalog varies by country due to manufacturer restrictions. If you change the Ship-To country, some or all of the items in your cart may not ship to the new destination.