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Non-lethal ways to get rid of mice
Humane Mouse Removal
TDS Reader Contributors
Related Articles
Eliminating Field Mice
Getting Rid of Mice
Mouse Removal Tools & Traps
Scarecrows for Mice?
I've got mice in my garage. As I have to store car seats, kid toys, and other things I don't want doused with mouse wastes, I'm looking for a way to drive them out, short of getting a garage cat. Any "scarecrows" or other non-lethal ways of driving them off?
Marc B
Predator Urine
We have often used and heard of others successfully using Predator Urine to rid their yards, garages, etc. of varmints! We are fortunate that our local hardware store carries these products but if yours does not here is the Web Site: PredatorPee.com They seem like nice people and in fact referred us to a local distributor thus saving shipping costs!
Cats Are Still Best
Get a cat, the living mousetrap. The cat will never get tired of going out into the garage to find mice. The ones she kills, she will bring to you and the ones she doesn't will have run off and stayed off, for the smell of cat will keep them away. On top of this expert 'round the clock service you will have a barrel of fun playing with the cat indoors. You will hardly even notice the cat taking over your life and your home. By then you will not care. Tip: Get your mouser at a local animal shelter. Ours had to put down 600 unwanted cats last month but gives coupons for vet services for those who are adopted.
Instead of a Cat
This solution does work, and it is short of getting a cat. Get some USED kitty littler from a friend's cat. place it in small tubs (margarine) in several places around the garage. Mice think "smell like cat--must be a cat". Same with gophers pour it down the hole. Wear gloves and do not use if you are expecting.
Blanch T.
Remove Food Source
The best solution for getting rid of mice is to get rid of their food source. Find out what is attracting the mice to the garage and get rid of it or seal it up. We had a mouse problem in our home and did this. We put our garbage in a sealed container, wiped up any food spills, sealed up pet food, and didn't leave food setting out. Our mouse problem disappeared!
Melissa G
Moth Balls
I use this method and it keeps my garage completely clear of all living creatures. Buy a net laundry bag for washing nylons and other delicate fabrics at Wal Mart. Buy 5 boxes of moth balls. Put them in the bag and hang it in your garage near the door. That's all there is to it. I use this in my out building and in the storage facility that I rent. No bugs, no mice, and no birds.
editor's note: moth balls are poisonous to children, pets and other small creatures. So make sure that they're hung out of reach.
Chew Proof
To prevent mice from gaining entry to your garage, stop up any openings (i.e., around door frames, holes in baseboards, etc) with steel wood pads. Mice will not chew through these!
Humane Traps
I thought up a way to help my mother with her mouse problem, and it worked pretty good, without killing the mice. Build a "pit trap." You need a narrow,deep, smooth sided container- a five gallon bucket might work, but make sure it has no scratches on the inside walls. You place a ramp up to the lip of the bucket and place some sort of smelly bait (peanut butter, cheese) in the bottom. It works best if you have some sort of lid on the bucket with a hole about 3 inches in diameter that the mice can drop down through, as mice can jump rather high. This way, they will hit the lid when trying to leap out. If the bucket has any scratches or grooves on the inside, the mice will be able to climb out.
To release them, you simply turn the bucket on its side with the hole at the bottom. Make sure you are some distance from your house (and anyone else's) when you release them, or they may move right back in! Depending on your mouse problem, you may have to do this several times.
Pepper Liberally
This trick works great and won't kill the mice: sprinkle ground cayenne around the garage (inside & out), and especially near entry holes. The critters can't stand the stuff!
Try Pellets for Humane Mouse Removal
Having lived on dairies and in rural areas for all of my adult life, I have had to deal with mice on occassion. I found my answer in a product called *Just One Bite*. I have never in the years I've used it had a mouse die in the house. I put the pellets out in jar lids and within 2 or 3 days the mice are gone. I can't recommend it highly enough!
Katharine W.
see The Ultimate Green Store - your one-stop green shopping destination
A 'Good-Bye' Mint
Mice hate the scent of peppermint extract. Simply sprinkle *pure* peppermint extract on the items in your garage you don't want the mice in or on. A friend of mine has used this in the interior of his antique car as well as on it's upholstery and never had a problem. It needs to be repeated monthly.
Take the Next Step:
- Looking for affordable pest control solutions? DIY pest control could be just the answer! (affiliate link)
- Could spending 5 minutes reading a newsletter twice a week save you time and money every day? Dollar Stretcher Tips readers think so. Subscribe and find out how many ideas stretch your day and your dollar!
Also in Home
- 6 cheap, effective home security solutions
- 5 simple and affordable luxuries for your home
- 5 frugal ways to expand your living space
- Top 10 DIY mistakes made by home 'handymen'
- Tha basics of financing a home improvement project
- 4 secrets to budgeting for a home purchase
- Winter energy saving ideas for renters
- How to control carpenter ants
- 3 things you need to know about free firewood
- Essential fall home repairs
- Fill your home with things that you love
- Organize your garage
Helpful Resources
- Find the best mortgage rates in your area
- 3 ways to use a mortgage calculator
- Mortgage calculator: Calculate your payment and more
- Home equity calculator: HELOC vs. line of credit
- Should I borrow from my home equity?
- How much can additional payments save me on my mortgage?
- Who offers the most home insurance discounts?


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NEW YORK, June 19 /PRNewswire/ -- WallSt.net's 3-Minute Press Show is a daily video program hosted by WallSt.net reporter, Tracee Tolentino.
Shows air Monday through Friday on: http://tv.wallst.net.
WallSt.net's 3-Minute Press Show features in-depth interviews with public company executives on their company and most recent press releases. The show is designed to provide viewers with insight into a company's most recent press release, and its impact on the company's growth.
The following executives were interviewed on today's show:
Han Tjan, Head of North American Corporate Communications for Daimler AG (NYSE: DAI) (http://www.daimler.com). To view this clip in its entirety, visit:
David McWilliams, President and CEO of Opexa Therapeutics, Inc. (Nasdaq: OPXA) (http://www.opexatherapeutics.com). To view this clip in its entirety, visit:
Declan Byrne, Chief Marketing Officer of Airspan Networks, Inc.
|SOURCE WallStreet Direct, Inc.; Opexa Therapeutics, Inc.;BrainStorm Cell
Copyright©2008 PR Newswire.
All rights reserved

subset: commoncrawl


text:
The new Yardbarker app is here! Install on your iPhone, iPad or Android device. You can also find us in Google Newsstand.
FSU Basketball Player of the Week: Devon Bookert
Florida State split a pair of contests this week as the Seminoles defeated Boston College on Wednesday before falling to No. 2 Virginia on Sunday night. It wasn't a week filled with great individual performances, but Devon Bookert was clutch in Florida State's home victory on Wednesday. Bookert is the Florida State Basketball Player of the Week for the week of February 16th through...
Seminoles' Rally Falls Short vs. Duke
Florida State made another furious rally at home on Monday night, but in the end, it was too little, too late as the Seminoles came up on the short end of a 73-70 score against No. 4 Duke. Devon Bookert set a new career-high with 23 points on 6-for-8 shooting against the Blue Devils and tied a season-high with 6 assists as FSU (13-12, 5-7 ACC) whittled what was a 14-point Duke lead...
Seminoles beat Gators on accidental final-second tip-in
TALLAHASSEE, Fla. -- Florida forward Jacob Kurtz accidentally tipped in a Devon Bookert miss with 0.4 seconds left to give Florida State a 65-63 win against its in-state rival. The victory Tuesday snapped a Gators' five-game win streak in the series dating back to 2008. The fluke play saved the Seminoles after they gave up an alley-oop from Kasey Hill to Dorian Finney-Smith with...
Via AP on Fox
December 30, 2014 | Discuss
Seminoles save their best for March
TALLAHASSEE, Fla. -- Florida State has saved its best basketball for late March. Unfortunately, it was in a Monday night NIT game and not a Sunday night NCAA Tournament game. But impressive, regardless. Devon Bookert, for a long stretch, couldn't miss a shot and was spot on with his passes on what was a career-high 21-point night. Aaron Thomas made 8 of 10 shots and had 26 points...
Via Fox Sports Florida
March 25, 2014 | Discuss
FSU Basketball: Seminoles using NIT to build for next season
Devon Bookert scored 21 points in Florida State's win over Georgetown on Monday. (Garry Thompson/Sneakin' Deacon) Early NIT success has FSU with promising outlook for 2014-15 For a team like Florida State that sat on the bubble on Selection Sunday, it was a mystery as to how the Seminoles would respond at the opportunity of playing in the NIT. After a lackluster 58-53 victory...
Via isportsweb.com
March 25, 2014 | Discuss
FSU keeps tourney hopes alive with GT win
Devon Bookert scored 16 points off the bench, going 4 for 4 on 3-pointers, as Florida State defeated Georgia Tech 81-71 on Sunday.
Via Philly Sports Live
Facing down must-win situation, FSU delivers against Georgia Tech
TALLAHASSEE, Fla. -- Florida State coach Leonard Hamilton didn't want to say it but he voluntarily admitted that the Seminoles were in a "must" situation on Sunday night. Must win. Must gain confidence. Must build some momentum in the final few games of the regular season. Florida State delivered. Okaro White scored 18 points and Devon Bookert came off the bench to add 16 points...
Via Fox Sports Florida
Okaro White, Devon Bookert lead FSU past Georgia Tech at home
TALLAHASSEE, Fla. (AP) -- Devon Bookert scored 16 points off the bench, going 4 for 4 on 3-pointers, as Florida State defeated Georgia Tech 81-71 on Sunday. The Seminoles (17-11, 8-8 ACC) kept their slim NCAA tournament hopes alive with the win, with two regular-season games remaining. Okaro White finished with 18 for Florida State as Aaron Thomas added 15. Montay Brandon grabbed...
Via Fox Sports Florida
Florida State Records a Triple-Dribble in Loss to Minnesota
After watching the Florida State/Minnesota game Tuesday night Washington Redskins fans can rest a little easier with the knowledge that they aren't the only ones getting screwy refs.From jimmyv.orgTuesday night the Seminoles took on the Gophers and at one point it appeared that Devon Bookert (I think; kind of hard to see his number clearly) picked up the ball and then started to...
Via FatManWriting
December 04, 2013 | Discuss
FSU beats Northeastern in dramatic fashion 62-60
Devon Bookert hit a 10-foot fade away jumper off a missed layup with 0.5 seconds to give Florida State a dramatic 62-60 win over Northeastern in the third place game of the Puerto Rico Tip-Off on Sunday. Northeastern's Scott Eatherton got off a desperation 3-pointer from the top of the key at the buzzer, but it bounced harmlessly off the backboard. Aaron Thomas had 16 points to...
Via AP on Fox
November 24, 2013 | Discuss
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Air Quality Regulations Companies in Kiribati
Category: Air & Climate ×
Subcategory: Air Quality Regulations ×
Location: Australasia ×
Location: Kiribati ×
Emissions Guru Inc.
based in Yorba Linda, CALIFORNIA (USA)
Emissions Guru Inc. provides emissions compliance and reduction solutions for On-highway vehicles and Off-road equipment. We also provide `go to market` services for emission control technology manufacturers and / or companies wishing to sell, install and service emissions control devices.
EMIT Environmental Brokers (EMIT)
based in Melbourne, AUSTRALIA
EMIT Environmental Brokers (EMIT) is an Australian based origination, advisory and transaction services company operating in the emerging global carbon trading and renewable energy markets. Formed in 2005, EMIT operates in both the regulated and voluntary carbon trading markets with an emphasis on developing market based solutions to assist clients manage their market ...
Shield Air Solutions Inc.
based in Houston, TEXAS (USA)
Shield Air Solutions, Inc. is the global leader in the design and manufacturing of explosion proof HVAC systems. In 2009, Shield Air was formed by uniting two of the industry's most well-known hazardous location HVAC manufacturers into one company. With more than 85 years of collective expertise in providing industrial grade, hazardous duty equipment, Shield Air is the ...
Environmental Monitor Service, Inc.
based in Yalesville, CONNECTICUT (USA)
Environmental Monitor Service Inc. is a leader in the field of advance technology Opacity and Dust Monitor Systems. EMS' EPA Compliant Opacity and Non-compliant Opacity Monitors are guaranteed to exceed CFR 40, Part 60 Performance Specification 1 for Opacity Monitors as well as ASTM D 6216. We offer a wide variety of models to fit every budget and suit any application. ...
STEAG Energy Services LLC
based in Kings Mountain, NORTH CAROLINA (USA)
STEAG Energy Services provides a full scope of environmental engineering and consulting service to the North American energy industry including SCR/SNCR system design and review, SCR catalyst management services including catalyst data interpretation, catalyst exchange strategies, catalyst cleaning, rejuvenation and regeneration, STEAG's patented/ pleated large particle ...
Pem-Tech, Inc.
based in Sugar Land, TEXAS (USA)
PemTech offers world-class quality and unparalleled customer service with our full range of industrial gas detection systems. Our industrial gas detectors and wireless gas sensors are equipped to handle a variety of toxic and combustible gases. We have detectors specifically designed to monitor Carbone Dioxide (CO2), Hydrogen Sulfide (H2S), various combustible gases and ...
based in Montmeló ( Barcelona ), SPAIN
The brand name of Prisma Actuators has become well-known all around the world and is now a point of reference in this industry because of the robustness and reliability of its products. This success relies on continuously improving the quality and enhancing the development of our products and also to tailoring our service to meet the individual requirements of every one ...
Envirocare International, Inc.
based in American Canyon, CALIFORNIA (USA)
For 30 years, EnviroCare International has designed and manufactured pollution control systems across a wide range of industrial and municipal applications for clients worldwide. Each of our over 1,000 installations is custom engineered and fabricated to meet the exact specifications of our clients and their individual applications. We offer advanced solutions to the ...
PPC Air Pollution Control Systems
based in Longview, TEXAS (USA)
PPC specializes in MACT compliant air pollution control using different methods. These methods include using biofilters and biofiltration for VOC control and wet and precipitator and dry electrostatic precipitator devices ( ESP ) for particulate. The biofilter, bioscrubber and biofiltration products are manufactured for the abatement of odors and the destruction of VOC's ...


text:
Originally posted by PatrickGarrow17
Atlantis existed
edit on 12/12/2012 by PatrickGarrow17 because: (no reason given)
My opponent said the following:
[ quote]Without understanding what consciousness is, the hope of creating AI is futile. We simply cannot create a machine to mimic us flawlessly without first understanding ourselves.[/ headline]
If your child's DNA tested positive for Tay-Sachs, and there was a gene therapy treatment available, would you cure your child?


text:
SAN DIEGO and SUNNYVALE, Calif., April 16 /PRNewswire-FirstCall/ -- Pharmacyclics, Inc. (Nasdaq: PCYC) today presented preclinical results demonstrating the anti-tumor activity of PCI-27483, the company's small-molecule Factor VIIa inhibitor. The data were presented during the Annual Meeting of the American Association for Cancer Research (AACR) being held this week in San Diego, CA.
Researchers reported that in animal models of human pancreatic tumors, treatment with PCI-27483 led to inhibition of tumor growth. PCI-27483 was administered twice daily at either 60 mg/kg or 90 mg/kg, resulting in 42 percent and 85 percent inhibition of tumor growth, respectively, after 15 days of treatment. Additional mechanistic studies showed that PCI-27483 blocked key tumor growth signals. Previous work has shown significant growth inhibition in lung and colorectal cancer models. Pharmacyclics anticipates filing an IND for PCI-27483 in the second half of 2008.
"These results support our IND-enabling studies for use of PCI-27483 in treatment of cancer and to protect against associated thromboembolic diseases," said David J. Loury, Ph.D., vice president of preclinical studies for Pharmacyclics. "These findings indicate that Factor VIIa inhibition is potentially a novel treatment approach for patients whose tumors depend on Factor VIIa activity for progression."
Factor VII is a blood protein involved in clotting. Many types of
cancer, such as lung, breast, pancreatic, colorectal, gastric and others,
express high levels of a cell surface protein known as tissue factor. After
binding to tissue factor, Factor VII becomes activated and triggers a host
of physiologic processes that facilitate the growth, invasion and spread of
many tumors, a
|SOURCE Pharmacyclics, Inc.
Copyright©2008 PR Newswire.
All rights reserved


text:
2004 Dodge Caravan Repair and Maintenance
A guide to problems, costs, maintenance and repair for your 2004 Dodge Caravan
- Our car conks out when stopped. Runs fine until stopping, then the idle stops
- takes 15 min to heat up, before that I put transmission on D , it doesn't move.
- My 2004 dodge caravan looses power what should i do?
- van won't shift up. rpm = 5000 at 60 mph
- P0016 code? Replaced both sensors but same code, now it doesn't want to accel
- Radiator Replacement $428 - 697
- Tie Rod End Replacement - Inner $160 - 520
- Tie Rod End Replacement - Outer $142 - 575
- Axle/Half Shaft Replacement - Front $479 - 1,417
- Fuel Pump Replacement $303 - 491
- Ray & Dana's Inman Auto Care (732) 856-5054 | 3.46mi
- 12 Volt Doctor (718) 412-9553 | 2.98mi
Related Content
2004 Dodge Caravan Problems
Worn sway bar bushings or sway bar end links may cause a rattling or clunking noise from the front suspension during low-speed driving. Though not always the root cause, front struts are often replaced for this condition.
Due to the crankshaft end play being out of specification, the front crankshaft seal can be popped or pushed out. Revised crank main thrust bearings will solve the problem.
Some vehicles may develop a musty odor coming from the HVAC system, Dodge has issued a cleaner/disinfectant and a coating for the evaporator designed to inhibit bacterial growth.
2004 Dodge Caravan Questions
When your drive it, it feels like somethings holding it back. Misfiring/ pulsing at 1500 rpm. Thinking a timing issue or what?
I replace the alternator belt and spark plugs today but the mechanic stated the plugs was wet with gas and thats why it's so hard to start. But all day I had no problem starting it. What would cause that and is it expensive to fix?
a/c leak water into passage floorboard when I drive it or run it 10 or 15 minture why
2004 Dodge Caravan Recalls
2004 Dodge Caravan Reviews
check engine will not go off everyone said it was the gas cap bought a new one and after two weeks it is still on.
2004 SXT 20th Anniversary Edition, 154,000 miles: Great van, crappy brakes -- 4 wheel disc . Front rotors prone to warping. Have been told that the brakes aren't big enough for the van's 4300 lbs. All electronics work great -- DVD player etc. Power sliders quit at 100,000 and 120,000. Water pump leaks a tad, but what the hell, we've driven the piss out of it. Suspension parts were replaced earl...


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Sorry, no definitions found.
Sorry, no etymologies found.
The scriptural usage of the word sarx is very extensive.
They also pointed out the correct meaning of the Scriptural passages alleged by Apollinaris, remarking that the word sarx in St. John, as in other parts of Holy Writ, was used by synecdoche for the whole human nature, and that the true meaning of St. Paul (Philippians and
A Sarcoma (from the Greek 'sarx' meaning "flesh") is a cancer of the connective tissue (bone, cartilage, fat) resulting in mesoderm proliferation.
WN.com - Articles related to Physicians in Qatar work on treatment of ovarian cancer
Sarcoidosis, also called sarcoid (from the Greek sarx, meaning "flesh") or Besnier-Boeck disease, is a multisystem disorder characterized by non-caseating granulomas (small inflammatory nodules).
The word "sarcopenia" is derived from the Greek root words sarx, meaning "flesh," and penia, meaning "loss."
The NIV translates sarx or the flesh as sinful nature.
TEXAS FAITH: Do disorders of the mind stop us from being moral actors? | RELIGION Blog | dallasnews.com
ORIGIN mid 16th cent.: from French sarcasme, or via late Latin from late Greek sarkasmos, from Greek sarkazein 'tear flesh,' in late Greek 'gnash the teeth, speak bitterly' from sarx, sark- 'flesh' .
Beowulf, is there some reason you believe that the words ho logos sarx egeneto couldn't bear such an interpretation?
* Ei poinun he sarx he despotike, to kuriakon plasma, ho xenos anthropos, ho ouranios, to neon blastema, to apo tes xenes hodinos anthesan houtos lambanei to pneuma hagion: [4912] 1
[72] Ei poinun he sarx he despotike, to kuriakon plasma, ho xenos anthropos, ho ouranios, to neon blastema, to apo tes xenes hodinos anthesan houtos lambanei to pneuma hagion, etc.


text:
Corrections and clarificationsIn our obituary of Lord Forte, page 40, March 1, we mistakenly said that the "Monico site" in central London housed the Criterion theatre. In fact the theatre is in the Criterion building on the other side of Piccadilly Circus.
In praise of... guilty pleasuresLeader: Lost rationalist values might be a worry, but other vanishings should be celebrated, and none more so than that of the fierce snobbery that for decades inhibited lovers of spirited music.
Country diaryVeronica Heath: Northumberland
Turning up the volumeLeader: In the best of all possible worlds, Zimbabwe has finally reached the tipping point that will bring about change. In the real world, little that happens in the fag end of Robert Mugabe's rule is either inevitable or irreversible.
How red is Brown?Leader: Budgets always have an element of theatre, and Gordon Brown's performance this week was more West End than Westminster.
Editorials & reply p40
We're not squeezing locals out of our Olympic plansWe are regenerating the area in partnership with those who live and work there, says David Higgins.
Foot, fraternity and the future of the EULetters: Footite Labour was by no means anti-European (In praise of ... Michael Foot, March 21). It was against the European Common Market of the day that was essentially a club for big farmers and bankers.
Hopes and fears over GM mosquitoes
Letters: The level of optimism displayed over the potential ability of GM Plasmodium-refractory mosquitoes to curb malaria transmission (GM mosquitoes offer new hope for millions, March 20) reminds one of a time after the invention of DDT, when mankind again set out to eliminate malaria.
The dog's blogLetters: The sad news that the Gay's the Word bookshop is fighting for survival is further evidence that the book trade is in danger of losing a rich tradition of independent, committed and knowledgable booksellers (Report, March 22).
Salvaging SmithLetters: Adam Smith, like other thinkers of his time, did regard indigenous peoples as "savage" (Letters, March 20), but also castigated the "savage injustice" that made trade between Europe and the Americas "ruinous" to south Asia and the Caribbean.


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1-16-15 Calendar
Winter Cribbage Tournament held by the Robert Leroux Council 10934, Knights of Columbus at St. Joseph in Belmont. Doors open at 5:30 p.m. followed by the game at 6 p.m. Entry fee of $15 per person.
Events at Gilford Public Library: Storytime, 10:30 a.m. to 11:15 p.m.; Social Bridge, 10:30 a.m. to 12:30 p.m.; Knit Wits, 1:30–2:30 p.m. Conversational German Class, 2:30–3:30 p.m.
"Joseph and the Amazing Technicolor Dreamcoat," 7:30 p.m., Winnipesaukee Playhouse, presented by Playhouse's Education Department. Tickets available at www.winnipesaukeeplayhouse.org or by calling (603) 279-0333.
Cribbage Tournament, 6 p.m., St. Joseph Church Hall, 96 Main St., Belmont; sponsored by Robert Leroux Knights of Columbus Council 10934.
Multimedia tour of Spain titled "Spain: A Magical Mystery Tour" presented by a UNH Spanish professor, 7 p.m. at the Rey Center in Waterville Valley as part of its Winter Lecture Series.
Al-Anon Meeting at the Congregational Church Parish House (18 Veterans Square) in Laconia. 9:30 to 11 a.m. each Friday. Al-Anon offers hope and help to families of alcoholics. No dues or fees. All are welcome. Call 645-9518.
Giggles & Grins playgroup at Family Resource Center in downtown Laconia (719 No. Main Street, Laconia). Free group for parents children from birth through age 5. For more information call 524-1741.
Friday Night Meltdown Public Skating event held at the Laconia Ice Arena from 6:45 to 8:15 p.m. Features Live DJ, Public Skating, laser light show, games and prizes. Open for all ages. $10 per person.
Events at the Gilman Library in Alton: Story time with Miss Lily, 9:15 a.m.; Story time with Granny Apple, 2:15 p.m.
Zentangle Workshops at the VynnArt Gallery in Meredith. For more information or to sign up call 279-0557.
Events at Gilford Public Library: Tot Time Reading Circle at the Meredith Library 9:30-10:30 a.m.; Young Writers Group at the Meredith Library 3:30-4:30 p.m.
Last Updated on Thursday, 15 January 2015 09:04
Hits: 251
1-15-15 Calendar
Lakes Region Chamber of Commerce annual Membership Meeting, noon-1:30 p.m., Margate Resort, Laconia.
Program about "Birding Marvelous Morocco and the Canary Islands," 7 p.m. at the Loon Center in Moultonborough. Refreshments will be served.
Lakes Region Builders Members Meeting, 5-8 p.m., Huot Technical Center. Join the discussion on Worker's Comp and why reform is needed. Also 2015 Scholarships Available for qualified students! $15/members and $20/non-members, includes dinner & tours of Huot Tech School. Contact: 387-1817.
Events at the Gilford Public Library: Toddler Time (Ages 2-3 years), 10:30-11:15 a.m. Brown Bag Book Discussion, 12:30 pm – 1:30 p.m.; Open Painting at the Library, 1:30–3:30 p.m.; Make It! Crayon Creations (Teens), 3-4 p.m.; Conversational French Class, 3:30–4:30 p.m.; Crafter's Corner, 6-7:30 p.m.; Brown Bag Book Discussion, 6:30–7:30 p.m.
Guys' Night Out, Gilford Community Church, 6 p.m. Cost is $12 per person. To make a reservation call 524-6057.
Craft Night at Belmont Library, 5:30 p.m.
Al-Anon Meeting at the Congregational Church Parish House (18 Veterans Square) in Laconia. 8 to 9:15 p.m. each. Al-Anon offers hope and help to families of alcoholics. No dues or fees. All are welcome. Call 645-9518.
American Legion Post 1 Bingo, 849 N. Main Street in Laconia. Doors open at 4 p.m. Bingo starts at 6:30.
Giggles & Grins playgroup at Family Resource Center in downtown Laconia (719 No. Main Street, Laconia). Free group for parents children from birth through age 5. For more information call 524-1741.
Visit the Gilman Library in Alton on Thursday evenings at 6 p.m. for a thought provoking game of chess and Pajama Story Time with Miss Bailey. Boards and game pieces for chess will be provided.
Thursday evenings at 6 p.m. Pajama Story Hour with Miss Lily will be held at the Gilman Library in Alton. Attendees are encouraged to wear pajamas and bring a teddy bear.
Families Sharing Without Shame, an open meeting for parents to discuss their child's drug addiction, alcoholism and recovery. 7 to 8:30 p.m. on Thursdays, except Holidays, Concord Hospital's Fresh Start Therapy Room. For more information call 568-0533.
Little Learners Pre-School Skating Lessons, 11:30 a.m to noon. Price is $10 per person, which includes a lesson and skating during public skate. Adult chaperones must pay an admission fee of $3. Rental skates are $5. For more information visit www.laconiaicearena.com.
Last Updated on Wednesday, 14 January 2015 08:12
Hits: 222
1-14-15 Calendar
Events at Gilford Public Library: Line Dancing for Beginners, 9-10 a.m.; Check–Out–An-Expert!, 10 a.m. to noon. Friends of the Gilford Library Monthly Meeting, 6:30–7:30 p.m.
Meeting held by the Belknap Mill Society to discuss the financial challenges facing the society's ownership and operation, 7:30 p.m. at the Belknap Mill in Laconia.
Preschool Story time and Craft at Belmont Public Library, 10:30 p.m.
10th Annual Keep the Heat On fundraiser in Plymouth State University's Prospect Hall. For more information call 238-2995.
SCORE Lakes Region holds a workshop designated to help business better understand the implications of various tax laws, 5:30 p.m. at the Lakes Region Chamber of Commerce offices in Laconia. Doors open at 5 p.m. Cost is $25 per person if paid in advance or $30 at the door. To register visit http://lakesregion.score.org/localworkshops.
Hebron Hazard Mitigation Plan Committee meeting to update the 2009 Hazard Mitigation Plan, 1 p.m. in the Public Safety Building located at 37 Groton Road in Hebron. For more information call 744-5584.
Workshop on how comics can bring history to life titled "Drawing from the Past: Using Local History Sources to Inspire Learning," 9 a.m. to 3 p.m. at the Laconia Public Library.
Laconia Elders Friendship Club meeting. 1:30 p.m. at the Leavitt Park Clubhouse. People 55 and older meet each Wednesday for fun, entertainment and education. Meetings provide an opportunity for older citizens to to meet for pure social enjoyment and the club helps the community with philanthropic work.
Country Acoustic Picking Party at the Tilton Senior Center. Every Wednesday from 7-9 p.m.
Duplicate bridge at the Weirs Beach Community Center. 7:15 p.m. All levels welcome. Snacks.
Preschool story time at Belmont Public Library. 10:30 a.m.
Hands Across The Table free weekly dinner at the St. Andre Bessette Parish Hall on Gilford Avenue in Laconia. Doors open at 4, dinner served at 5. All are welcome.
Overeaters Anonymous offers a program of recovery from compulsive eating using the Twelve Steps and Twelve Traditions of OA. Wednesday nights at 5:30 p.m. at St. Joseph Church in Belmont. Call/ leave a message for Elizabeth at 630-9969 for more information.
Zentangle workshop held every Wednesday from 5-7 p.m. at the Vynart Gallery located at 30 Main St., Meredith. For more information call 279-0557.
Narcotics Anonymous meeting. 7 to 8:30 p.m. at 18 Veterans Square in Laconia.
TOPS (Taking Off Pounds Sensibly) group meeting. 5:30 p.m. at the First Congregational Church in Meredith.
Last Updated on Tuesday, 13 January 2015 08:03
Hits: 257
1-13-15 Calendar
Events at Gilford Public Library: Social Bridge, 10:30 a.m. to 12:30 p.m. Story time 10:30 – 11:15 a.m. Babygarten, 11:15–11:45 a.m. Writing Expert, 3-5 p.m.; Gilford Clickers Photography Club, 6:30–7:30 p.m.
Events at the Hall Memorial Library: Spanish Club, 10 a.m. Tech Tuesday, 2-4 p.m. Sharpen your technical skills on that new device! Make an appointment by calling 286-8971 or inquire at the circulation desk.
Events at Belmont Public Library: TAG Team, 4 p.m. followed by Game Night at at 5:30 p.m.
Story time at Belmont Public Library. 3:30 p.m.
The New Horizons Band of the Lakes Region meets every Tuesday at 7 p.m. at the Music Clinic on Rte 3 in Belmont. All musicians welcome. For more information call 528-6672 or 524-8570.
Al-Anon Meeting at the Congregational Church Parish House (18 Veterans Square) in Laconia. 8 to 9:15 p.m. each. Al-Anon offers hope and help to families of alcoholics. No dues or fees. All are welcome. Call 645-9518.
Giggles & Grins playgroup at Family Resource Center in downtown Laconia (719 No. Main Street, Laconia). Free group for parents children from birth through age 5. For more information call 524-1741.
Unitarian Universalist Society of Laconia - Tuesday Night Meditation Group, 6-7:15 p.m. at 172 Pleasant St., Laconia. This group is designed to create a community where individuals can develop their own meditation practice in a friendly, non-judgmental community. No experience necessary. Sessions consist of a combination of sitting and walking meditations and weekly discussions (no preparation required). New comers are encouraged to arrive 10 minutes early for a quick introduction. No Charge. Free will donations accepted.
Last Updated on Monday, 12 January 2015 09:25
Hits: 330
1-12-15 Calendar
Events at the Gilford Public Library. Yarn Therapy, 9-10:30 a.m.; Mahjong, 12:30-3 p.m.
Events at the Hall Memorial Library: Chess Club, 4-7 p.m.; Art Opening featuring Water Photos produced by Hanz Busch, 6 p.m. Refreshments served.
Senior Moment-um program featuring Bingo at the Community Church, noon. Coffee and tea provided, participants are asked to bring their own lunch. RSVP by calling 527-4722.
Narcotics Anonymous meeting. 7 to 8:30 p.m. at 35 Tower Street in Weirs Beach.
Overeaters Anonymous offers a program of recovery from compulsive eating using the Twelve Steps and Twelve Traditions of OA. The program is held Monday nights at 7 p.m. at the Laconia Congregational Church Parish Hall, 18 Veterans Square, (for mapquest use 69 Pleasant St.), Laconia, NH 03246. Use back entrance. Call/leave a message for Paula at 998-0562 for more information.
Barbershop chorus meets every Monday at Gilford Community Church, lower level. 7:15 p.m. For more information, call Harvey Beetle at 528-3073.
Chess Club at the Hall Memorial Library. 4-7 p.m.
Free one on one internet and computer instruction every Monday at 10 a.m. at the Tilton Senior Center, 11 Grange Road, Tilton.
Adult Pick-up Basketball offered by Meredith Parks & Recreation Department held at the Meredith Community Center Monday nights from 6 p.m. to 7:45 p.m. $1 per person - sign in and out at the front desk.
Bingo at the VFW Post 1670 located at 143 Court Street in Laconia. Doors open at 4 p.m. Bingo begins at 6:30 p.m.
Families Sharing Without Shame support group for parents who have a child/children who have or currently are abusing drugs and/or alcohol, 7-8:30 p.m. in the Gilford Community Church Gathering Room. The group's goal is to provide a safe meeting place where there is no guilt, shame, or blame, just compassion and understanding.
Last Updated on Friday, 09 January 2015 09:18
Hits: 328


text:
LÆOrķal Earns Patent For Self-Tanner
Published November 15, 2005
Related Searches: composition • skin • patent • men •
Patent No. US 6,740,313 B2: L'Oréal SA developed a patent for a cosmetic and/or dermatological composition that produces a long-lasting and non-covering tan on the skin. It comprises a cosmetically acceptable medium and at least one pigment obtained from a Monascus micromycetes extract.
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text: alina967Threads: 11Posts: 43Author: Archana Sevak Nov 22, 2009 #1Today an online class and traditional class are two choice for students. Sometimes students confuse to decide that which class is better for them. The traditional class and an online class offer interesting three points of similarities and difference to the students in three area of environment of classroom, teaching instruments, and communication to the students. Students have a good study environment in traditional ...SEE BELOW


text:
Soto vs Oscipcziak at UFC 118 in Boston
Recent Comments »
5/5/10 2:38 PM by Mikey2004
Nick Oscipcziak vs Greg Soto Andre Winner vs Nik Lentz Terry Etim vs Joe Lauzon A few Brits fighting at 118, Boston Tea Party anyone?
5/5/10 2:16 PM by NHB USA
Damn I wanted Sodallah vs Osipcheck
5/5/10 1:22 PM by SandraBullockFan
damn i missed that fight!!! i thought it was on this ppv
5/5/10 1:21 PM by thforklift
Soto - all day.
5/5/10 1:19 PM by AC Slater
Losing by decision to Rick Story isn't a tremendous setback, Story is very underrated for his division. And Nick should easily take Soto.
5/5/10 1:18 PM by BanTheUKmmaNoobs
Controversial split decision in favour of Story.
5/5/10 1:14 PM by SandraBullockFan
what happened to slick nick vs story?
5/5/10 12:50 PM by BanTheUKmmaNoobs
Osipczak. Easily.


text:
For the new study, Redmond revisited nine pinyon pine study sites scattered throughout New Mexico and Oklahoma that had been studied previously in 1978 by Forcella. Both Forcella and Redmond were able to document pinyon pine masting years by counting small, concave blemishes known as "abscission scars" on individual tree branches that appeared after the cones have been dropped, she said.
Since each year in the life of a pinyon pine tree is marked by a "whorl" -- a single circle of branches extending around a tree trunk -- the researchers were able to bracket pinyon pine reproductive activity in the nine study areas for the 1969-1978 decade and 2003-2012 decade, which were then compared.
Pinyon pines take three growing seasons, or about 26 months, to produce mature cones from the time of cone initiation. Low elevation conifers including pinyon pines grow in water-limited environments and have been shown to have higher cone output during cool and/or wet summers, said Redmond. In addition to the climate-warming trend under way in the Southwest, the 2002-03 drought caused significant mortality in pinyon pine forests, Redmond said.
"Miranda's ideas and accompanying results will be of value to ecologists and land managers in the deserts of the Southwest and beyond," said Forcella, now a research agronomist in the USDA's Agricultural Research Service. "The work is evidence that the University of Colorado continues to cultivate a cadre of high-caliber graduate students for which it rightfully can take tremendous pride."
Pinyon nuts, the Southwest's only commercial source of edible pine seeds today, were dietary staples of indigenous Americans going back millennia.
|Contact: Miranda Richmond
University of Colorado at Boulder


text:
Kimble Beauty Bounce Back Curl Revitalizer
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text:
- Filed Under
STAUNTON - A contest for beautification dollars could help the Queen City add another jewel to her crown - but your vote is needed.
Staunton is one of three finalists in a national, nomination-driven contest called Henkel Helps. The city with the most votes by Friday wins the services of a 10-person cleaning crew from Henkel, the company that represents brands such as Dial, Purex and Soft Scrub.
Makeba Robinson nominated Staunton's Johnson Street for a Henkel-aided makeover, suggesting that the crew add more outdoor color and greenery and perhaps expand the neighborhood playground. ...


text:
CEO Has Special Incentive to Spend on Horizant, Says Clinton Group PR Newswire NEW YORK, June 5, 2014 NEW YORK, June 5, 2014 /PRNewswire/ --Clinton Group, Inc. ("Clinton Group") announced today that it has sent a letter to the stockholders of XenoPort, Inc. (Nasdaq: XNPT) in advance of the June 11, 2014 XenoPort annual meeting in which it expresses its view that XenoPort should focus on its core competencies, namely scientific discovery and clinical trials. The Clinton Group has nominated three independent professionals for the Board of Directors and is asking for the support of its fellow XenoPort stockholders. "In our view,XenoPort's spending on Horizant has cost stockholders significantly in the form of a lower stock price and dilution," said Gregory P. Taxin, President of Clinton Group. "Moreover, the analysts who cover the Company now regard Horizant as being less valuable than before XenoPort started spending its precious capital on the drug." In its letter to stockholders, Clinton Group notes that XenoPort's Chief Executive Officer has stock options that vest only if the Company achieves a commercial milestone with Horizant. Clinton Group says it believes this gives the Chief Executive Officer an incentive to spend XenoPort's capital on Horizant, even if such spending has a poor expected return. Clinton Group notes, by contrast, there does not appear to be any economic incentive for the Chief Executive Officer to partner or sell Horizant. "We believe Dr. Barrett's incentives are not aligned with stockholders," continued Mr. Taxin. "Perhaps that is why the Company spent $8 million last quarter on Horizant commercialization, only generated an incremental $220,000 in sales and wants to keep doing this. We would prefer to see this science-based company focus on science, not marketing drugs to consumers and doctors." Clinton Group encourages its fellow stockholders to review the materials it has prepared for stockholders, which are available at https://okapivote.com/clintonxnpt/. Clinton Group urges stockholders to vote for change at the annual meeting by using the GOLD proxy card. Stockholders with questions should contact Okapi Partners LLC at (212) 297-0720 or Toll-Free (855) 305-0857 or by email to email@example.com. A copy of the letter sent to XenoPort stockholders is included below: June 5, 2014 To Our Fellow Stockholders of XenoPort: I write on behalf of the Clinton Group Inc., which together with funds it manages ("Clinton Group"), is one of the largest investors in XenoPort, Inc. ("XenoPort" or the "Company"). As you probably know by now, we are unhappy with the Company's capital allocation decisions and stock price performance, and have nominated three independent professionals to the Board of Directors. We encourage you to vote for our three nominees, who, if elected, would be three new, objective directors on a Board of nine members. There is no question that the Company has not performed well for stockholders. For the five years ended yesterday, the stock is down more than 80%. In 2014 alone, the stock has declined more than 35% while the Nasdaq Biotechnology Index has risen 6%. The Company's stock has been moving, and continues to move, in the wrong direction. The Company's incumbent Board would have stockholders believe the Company is misunderstood or that the stock price once embedded unrealistic expectations. Hogwash. The stock has underperformed in nearly any period one examines, short- or long-term. It has underperformed even since the failure of one of the Company's compounds, arbaclofen placarbil, was priced into the stock about one year ago. The stock has underperformed since the Company decided to turn itself from a science-based enterprise into a full commercial company for the purpose of marketing Horizant, as well. The stock price performance actually tells the story. The Company has continually disappointed, and diluted, stockholders. To us, there is, unfortunately, no reason to think things will be different going forward without some fresh thinking and change on the Board. Our biggest disagreement with the incumbent Board boils down to whether this small, undercapitalized company should be spending its precious capital on building and operating a commercial effort for Horizant (the Board's contention) or whether XenoPort should find a partner who already has those expensive operations (sales, marketing, FDA compliance and such) in place, collaborate on Horizant, and then focus XenoPort on activities involving its core competency, scientific development and clinical testing. XenoPort has a very promising science and clinical project in XP23829. We believe it is a perfect focus for the Company, given XenoPort's historical strengths, personnel and core competence. There is no doubt that "829" will require significant additional capital for future clinical testing and development. We believe the Company should preserve its limited capital so that it can perform these future studies, de-risking the compound and creating additional value, through science, in 829. To maximize value for stockholders, the Company should not sell or partner 829 too early or sell additional equity in the Company, diluting the current stockholders' ownership of 829. We strongly believe that by focusing the Company's capital spending and human resources on 829, the Company will preserve its capital, develop 829 as much as possible, ensure the current stockholders continue to own all of 829 and then, at the optimal time, sell or partner 829. The alternative, supported by the incumbent team, is to spend the Company's limited capital to push the rock that is Horizant up a hill. Everyone agrees that the drug's initial launch in the United States was not a success. Whatever the cause, history is clear that it is very hard to re-launch a drug that has had an initial failed launch. The evidence from Japan (where the launch partner was different than the US partner) is also sobering: there, the sales appear to be declining and the Company's Asian partner has decided not to pursue marketing the drug in the five other Asian countries to which it had rights. Yet, the Company's management team and Board – the same people whose actions have brought the stock to its knees – insist that this small biotechnology company should perform a commercial experiment. They support spending tens of millions more to see whether Horizant is promotionally sensitive. If it is, they argue, they will be able to sell or partner Horizant for more money than they can get for it today. To us, there is no reason to think this is a good experiment. Pharmaceutical companies can see the data on Horizant already. They know the market and have read the research. They, like Wall Street analysts, can value the asset today based on all the probabilities of commercial success that one can know. The Company has no special insight into whether Horizant will succeed commercially or be "promotionally sensitive". And, of course, the Company has an extremely high cost of capital compared to pharmaceutical companies. In short, the Company has no objective reason to be more bullish than others and has a much higher cost of experimentation. And yet the Company pushes on. To us, this seems like bad economics. After all, if spending $30-$40 million on a Horizant commercial experiment was a good idea (i.e. it had great, risk-adjusted expected returns), it seems clear to us that there would be dozens of parties willing to run the commercial experiment (all of whom have a lower cost of capital and substantial existing infrastructure to lower the cost of the experiment) and pay XenoPort for the right to do so. Where are they? If they have approached the Company and the Company has not done a deal with them, it can only be because the Company believes it has special insight into how Horizant will perform commercially. Why would that be? The Company is filled with scientists, not marketers. If no other party has shown up to partner or buy Horizant, well, then, the Company again must believe it has special insight. Or, as we suspect, misplaced confidence in Horizant. We do not believe hubris should drive the allocation of shareholder capital. The Company has no rational basis that we can identify for believing Horizant will be more successful than other parties believe. This spending is therefore a gamble, not an investment. So far, the gamble has not worked. The analysts who cover XenoPort value Horizant lower today than they did before this commercialization experiment began.* With every dollar XenoPort spends, the analysts appear to realize there is no great commercial opportunity: growth in the prescriptions is too low and the spending is not effective. The experiment that only XenoPort thought was a good one, is failing. XenoPort saysit should not abandon its experiment even though $8 million in spending during the First Quarter only produced $220,000 in incremental Horizant revenue. We believe the Company should stop this unproductive spend. At the current pace, the Company will spend many millions more, prescriptions will grow just modestly and Horizant will be regarded as worth even less. Why not run a competitive process and let the market of smart, well-capitalized, checkbook-wielding pharmaceutical companies tell us what Horizant is worth? Why does the Board believe its unique view of value is right? What great insight does the Company have that it is unable to communicate to potential partners or buyers? We cannot help but offer one possible explanation. Dr. Barrett, XenoPort's Chief Executive Officer, has incentives that are different than those of stockholders. Half of Dr. Barrett's 2013 "performance-based" stock option grant vests only if the Company achieves a certain, unstated commercial milestone on Horizant. Dr. Barrett has an incentive to achieve that milestone – we suspect it is revenue or prescription-count based – no matter what it costs to do so. Dr. Barrett appears to get nothing if he sells or partners Horizant now. What is good for Dr. Barrett (already one of the highest paid executives at a biotech or health care company of this size) – continuing to commercialize Horizant at any cost and not selling or partnering the drug – is not necessarily good for stockholders. But it does appear to be the path the Company is on. We believe this small biotechnology company should not be attempting to prove commercial viability of Horizant. To us, it is a fool's errand that has already destroyed significant stockholder value and led to the need for additional capital, which diluted our ownership of 829. But even if we are wrong, we believe it would be enormously valuable to have some new professionals on the Board to help the Company consider these important capital allocation questions with a fresh perspective. Certainly, aligning incentives would be good. We believe value at XenoPort will be created by its scientific and clinical testing prowess. The Company has a great compound on which to focus those efforts and could spend all of its capital, productively, moving that compound through the various phases of testing and development. That is where, we believe, the Company can best spend its capital. Please return the GOLD proxy card and call Okapi Partners with any questions. They can be reached at 855-305-0857 or email@example.com. Sincerely, Gregory P. Taxin President * Source: Wall Street research. Credit Suisse lowered its per share valuation of Horizant from $5.50 to $3.00 in reports dated March 11, 2013 and May 8, 2014; RBC Capital lowered its per share valuation of Horizant from $5.00 to $3.00 in reports dated April 24, 2013 and May 9, 2014; Wells Fargo lowered its per share valuation of $3.00-$4.00 for Horizant/AP to $1.00 for Horizant in a report dated March 12, 2013 and as indicated in a model as of May 5, 2014. About Clinton Group, Inc. Clinton Group, Inc. is a Registered Investment Advisor based inNew York City. The firm has been investing in global markets since its inception in 1991 with expertise that spans a wide range of investment styles and asset classes. Important Additional Information CLINTON RELATIONAL OPPORTUNITY MASTER FUND, L.P., CLINTON MAGNOLIA MASTER FUND, LTD., GEH CAPITAL, INC., CLINTON RELATIONAL OPPORTUNITY, LLC, CLINTON GROUP, INC. AND GEORGE E. HALL (COLLECTIVELY, "CLINTON") AND KEVIN J. CAMERON, RAEL MAZANSKY, M.D. AND CHARLES A. ROWLAND, JR. (TOGETHER WITH CLINTON, THE "PARTICIPANTS") FILED WITH THE SECURITIES AND EXCHANGE COMMISSION (THE "SEC") ON APRIL 25, 2014 A DEFINITIVE PROXY STATEMENT AND ACCOMPANYING FORM OF PROXY CARD TO BE USED IN CONNECTION WITH THE PARTICIPANTS' SOLICITATION OF PROXIES FROM THE STOCKHOLDERS OF XENOPORT, INC. (THE "COMPANY") FOR USE AT THE COMPANY'S 2014 ANNUAL MEETING OF STOCKHOLDERS (THE "PROXY SOLICITATION"). ALL STOCKHOLDERS OF THE COMPANY ARE ADVISED TO READ THE DEFINITIVE PROXY STATEMENT AND OTHER DOCUMENTS RELATED TO THE PROXY SOLICITATION BY THE PARTICIPANTS BECAUSE THEY CONTAIN IMPORTANT INFORMATION, INCLUDING ADDITIONAL INFORMATION RELATED TO THE PARTICIPANTS. THE DEFINITIVE PROXY STATEMENT AND ACCOMPANYING PROXY CARD HAVE BEEN FURNISHED TO SOME OR ALL OF THE COMPANY'S STOCKHOLDERS AND ARE, ALONG WITH OTHER RELEVANT DOCUMENTS, AVAILABLE AT NO CHARGE ON THE SEC'S WEB SITE AT HTTP://WWW.SEC.GOV/. IN ADDITION, OKAPI PARTNERS LLC, CLINTON'S PROXY SOLICITOR, WILL PROVIDE COPIES OF THE DEFINITIVE PROXY STATEMENT AND ACCOMPANYING PROXY CARD WITHOUT CHARGE UPON REQUEST BY CALLING (212) 297-0720 OR TOLL-FREE AT (855) 208-8902. INFORMATION ABOUT THE PARTICIPANTS AND A DESCRIPTION OF THEIR DIRECT OR INDIRECT INTERESTS BY SECURITY HOLDINGS IS CONTAINED IN THE DEFINITIVE PROXY STATEMENT ON SCHEDULE 14A FILED BY CLINTON WITH THE SEC ON APRIL 25, 2014. THIS DOCUMENT CAN BE OBTAINED FREE OF CHARGE FROM THE SOURCES INDICATED ABOVE. SOURCE Clinton Group, Inc. Contact: N. Lennox, +1-212-825-0400
CEO Has Special Incentive to Spend on Horizant, Says Clinton Group
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text:
I need to run this by some people...
View Single Post
November 13 2012, 06:27 AM
Location: Detroit
Re: I need to run this by some people...
SonOfMogh wrote:
Man...... I haven't read all the other responses but you're massively overthinking this. I really wouldn't do this elaborate book thing, the bracelets were cool but there's a line between nice and desperate.
How is this even remotely coming off at desperate? Over thinking, definitely. Somewhat more excessive than is necessary, probably. But desperate? If I had asked her out already, was turned down, and I did stuff like this, then that would be desperate. Or, you know, begging and saying things like "Please just give me a chance!" which has the added bonus of being selfish and manipulative in addition to being desperate.
I keep forgetting that I haven't been very active over the past year or two, and there are probably people around that aren't familiar with me. Being over-the-top isn't just something I do when I'm trying to impress a girl I like. I'm always over-the-top and intense. If I'm not like this when interacting with women, that would be violating the "be yourself" rule. Those that have said "just ask her out and be simple about it" are, in effect, recommending that I put on an act. If this kind of thing is out of the ordinary for someone, acting over-the-top when interested in a girl is probably a mistake. That's not the case with me though, so it's cool when I do it.
Also, the thing with the bracelets was a good idea, no one is questioning that. (If they are, they're wrong.) She was talking about how she was supposed to have gone to the Renaissance Faire with this other girl in the class who dropped it early on and how it didn't happen. (It was over at that point, otherwise I would have suggested her and I go together.) So I thought, people that like Renaissance Faires generally like chainmail as well, I make chainmail, so I made her some bracelets. It was very thoughtful and an awesome thing for me to do. Of course, had I hesitated and made a thread about it, I'm sure some people would be telling me it was a creepy thing to do and I should just ask her out for coffee.
teacake wrote:
I'm thinking he is enjoying the overthinking.
It's more that I'm not capable of not over thinking things and I've gotten used to it. It's a pain in the ass sometimes, but it works for me, and is generally better than not thinking about something enough.
Gaith wrote:
Jeez, not even
Those things on pool tables, while they look like holes, are called pockets, so it's okay.
teacake wrote:
Actually I would prefer the thing you list as a nono.. coffee.
I've changed my mind, I need to read this guide now. If there's suggestions about where to go and what to do activity-wise, that could be useful and is a good idea. I also need to know what the reasoning behind coffee being a bad idea is. If it's a first date, I'd say that coffee isn't only a good idea, but the only acceptable one:
First dates usually involve a lot of talking, and coffee places are good for conversation.
Coffee shops have a very relaxed atmosphere. People are usually a bit nervous on first dates, so this helps.
There are no time restraints. If two people catch on early that they have no interest in each other, they can leave after ten minutes. If a very engaging conversation starts one can sit there for hours. Also, it's pretty easy to leave and go do something else if the mood strikes.
One for the ass holes: Even the most expensive coffee shop is still pretty cheap compared to other things. If the other person sits there an texts people the whole time rather than talking to you, you're only out $10.
A lot of people are concerned about date rape. As comedians liked to point out in the '90s, there's a Starbucks every few hundred feet. It's easy to meet up at one and then leave separately.
Most of this is true of bars as well, but drinking on a first date can be risky for a first date. Alcohol helps people relax, but it changes behavior in other ways, especially with judgment abilities. Say the date ends with sex. The next morning things might be fine, but it can also be awkward, and might even be rape.
The only downside I can really see to the coffee date is that lots of people do it, so it's boring and uncreative. Which, given everything else I've said, one would think I'd be against it. However, the benefits outweigh this one tiny downside. Also, boring and uncreative are two things I'm not, so I can easily work around it.
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text:
Transit users often feel like they're waiting about 50 percent longer than they actually are.
A new map showing the carbon footprint for nearly every zip code indicates that suburbs are powerful greenhouse-gas emitters.
Getting away from the job when you're always on the job.
But one website for all riders in the metro area is only a start.
Car culture may not be making us sick. Cities aren't necessarily healthier than suburbs.
Mayors open up about the stuff that gets them down.
A few have tried, but none have succeeded.
Mayors explain how friends and associates quickly become crucial advisers.
A different way of measuring what we spend on housing.
When it comes to social media at the municipal level, not everyone is a Cory Booker.
Turner Field hosted its first baseball game in 1997. It will host its last one in 2016.
You asked. Mayors answered.
Major urban areas are magnets for the uninsured, and the state politicians who turned down the Medicaid expansion are not the ones who will pay to treat them.
NYC's art-punk golden age, Chapel Hill's indie-rock community, and Memphis's Stax Records all declined in about the same way: The underdogs became the establishment.
And counting all of the people (and jobs) who have supposedly moved there.
What Atlanta's shifting real estate landscape might mean for the future of the country.
It offers up strange collection of "urban amenities," including shopping malls, and "futuristic" buildings.
Two ways to chart the connection between reading and place.
A menace coming to a nice community near you.


text:
Page 1
Biophysical Journal
Volume 65
July 1993
Internal Electrostatic Potentials in Bilayers: Measuring and Controlling
Dipole Potentials in Lipid Vesicles
J. Craig Franklin and David S. Cafiso
From the Department of Chemistry and Biophysics Program at the University of Virginia, Charlottesville, Virginia 22901 USA
vesicle systems formed from phosphatidylcholine. As a result of the membrane dipole potential, the binding and translocation
rates for oppositely charged hydrophobic ions are dramatically different. These differences were analyzed using a simple
electrostatic model and are consistent with the presence of a dipole potential of approximately 280 mV in phosphatidylcholine.
Phloretin, a molecule that reduces the magnitude of the dipole potential, increases the translocation rate of hydrophobic cations,
while decreasing the rate for anions. In addition, phloretin decreases the free energy of binding of the cation, while increasing
the free energy of binding for the anion. The incorporation of 6-ketocholestanol also produces differential changes in the binding
and translocation rates of hydrophobic ions, but in an opposite direction to those produced by phloretin. This is consistent with
the view that 6-ketocholestanol increases the magnitude of the membrane dipole potential. A quantitative analysis of the binding
and translocation rate changes produced by ketocholestanol and phloretin is well accounted for by a point dipole model that
includes a dipole layer due to phloretin or 6-ketocholestanol in the membrane-solution interface. This approach allows dipole
potentials to be estimated in membrane vesicle systems and permits predictable, quantitative changes in the magnitude of the
internal electrostatic field in membranes. Using phloretin and 6-ketocholestanol, the dipole potential can be altered by over 200
mV in phosphatidylcholine vesicles.
The binding and translocation rates of hydrophobic cation and anion spin labels were measured in unilamellar
Several distinct electrostatic potentials can be defined in lipid
bilayers that arise from a number of sources. For example,
transmembrane potentials result from charge separation
across the membrane, and membrane surface potentials re-
sult from charge that is bound to the membrane-solution in-
terface (1). Transmembrane potentials are known to regulate
the activity of certain ion channels, presumably as a result of
conformational changes in these proteins that are electrically
active. Although they have not yet been characterized, these
electrically active structural transitions must involve the
movement of protein charges or dipole moments within the
membrane interior so that conformational free energies are
dependent upon the transmembrane electric field. Surface
potentials represent a potential difference between the mem-
brane interface and the bulk aqueous phase. These potentials
are usually much smaller than transmembrane potentials, and
are typically on the order of a few tens of mV in biological
membranes; nonetheless, they appear to have an important
role in controlling the binding of charged proteins to mem-
branes. Indeed, surface potentials may control the activity of
enzymatic components of second-messenger systems, such
as protein kinase C, by regulating the binding of these pro-
teins to the membrane interface (2). In addition to trans-
membrane and surface potentials, lipid bilayers also possess
an internal potential termed a dipole potential. This potential
is much larger than either the surface or the transmembrane
Receivedforpublication 9December 1992 andinfinalfonn 12March 1993.
Address reprint requests to David S. Cafiso.
1993 by the Biophysical Society
potentials, and appears to be on the order of 300 mV hy-
drocarbon positive in phosphatidylcholine vesicles. The ex-
istence ofthe dipole potential has been known for some time,
both from electrical measurements in monolayers (3) and
work in model bilayers. In bilayers, it is believed to account
for the permeability differences of certain organic cations
and anions (4-10). Unlike the surface potential, the dipole
potential is independent ofionic strength; hence, it is thought
to result from oriented dipoles in the membrane interface.
The molecular source of the dipole potential has not been
clearly identified; however, carbonyl oxygens and/or water
at the membrane interface appear to be the most likely
sources of this potential. (11-13) A major role for water is
suggested by recent electrostatic calculations (14), as well as
by an apparent connection between the hydration pressure
and the dipole potential (12).
The magnitude of the dipole potential suggests that it
should be important in a number of processes. For example,
conformational changes in membrane proteins that result in
the movement of charge or dipole moments through the
membrane interface will be affected by the dipole potential.
The insertion of a-helical segments of proteins into mem-
branes should also be modulated by the dipole potential, and
this interaction may be an important energetic term affecting
the insertion ofpeptides or signal sequences into membranes
(15). The dipole potential is also expected to slow the trans-
location of positively charged segments of membrane pro-
teins during biosynthesis. As a result, the dipole potential
might account for the presence ofpositively charged residues
in stop-transfer sequences (16). Finally, virtually all efficient
protonophores are hydrophobic weak acids that shuttle
across bilayers in a negatively charged form (17). The lack
Page 2
Volume 65
July 1993
of any efficient weak base protonophores is likely the result
of the large the dipole potential, since these compounds
would need to shuttle charge in a positively charged form.
In spite of its likely importance, the role of the dipole
potential in controlling protein interactions with membranes
or its affect on membrane protein conformation has not been
demonstrated. This is a result of the fact that experimental
systems to examine these protein-membrane electrostatic in-
teractions have not been explored. The majority of studies on
dipole potentials in membranes have been carried out in pla-
narbilayer systems, and, while these systems provide a facile
means to monitor ion conduction, they do not readily permit
the measurement of events such as protein binding or the
characterization of protein structural changes. However,
membrane dipole potentials can also be estimated in lipid
vesicles where structural studies on proteins are also possible
An estimate ofthe dipole potential in vesicles can be made
by examining the membrane permeability and binding of
certain organic ions termed hydrophobic ions. Shown in Fig.
1 are the calculated free energy profiles for transferring the
hydrophobic cation tetraphenylphosphonium, #4P', and the
anion, tetraphenylborate 44B-, from solution across a lipid
bilayer in the absence of a surface potential. This energy is
estimated by adding together AGBOm,,the electrostatic Born-
Image energy, AGHYdO the (attractive) hydrophobic energy,
and AGDipole the energy of interaction of the ion with the
membrane dipole field (10).
AGO =AGBOm+AGOjPOIe + AGOYdrO
Distance from Bilayer Center (A)
ions tetraphenylborate (44B-) and tetraphenylphosphonium (44P+). These
energy profiles are calculated by adding together terms due to the electro-
static charging or Born-Image energy, the hydrophobic binding energy, and
the energy due to the dipole potential. The dipole potential makes these two
profiles dramatically different, a feature that accounts for the increased
binding and increased translocation rates of04B-when compared to
These profiles were determined as described previously (10), where the
intrinsic membrane dipoles have a strength of0.85 D with the density ofthe
lipid. The magnitude of the neutral binding energy was taken as -6.8 kcall
mol, with an ion radius of 4.2 and 4.0 A for 44B- and 44P+, respectively.
Free energy profiles across lipid bilayers for the hydrophobic
The large size of these ions lowers AGBOm,,, which makes
them quite membrane permeable. As a result of their hy-
drophobicity, the free energy profiles also show a minimum
within the membrane solution interface, and they are exper-
imentally observed to bind to a low dielectric domain of the
membrane (11). The differences between energy curves for
the hydrophobic cation and anion depicted in Fig. 1 are a
direct consequence of the dipole potential. As is readily ap-
preciated from Fig. 1, the dipole potential creates dramatic
differences in the translocation and binding of hydrophobic
ions, by altering the free energy barrier in the middle of the
bilayer and the depth of the free energy minima at the in-
terfaces. In fact, otherwise similar cations and anions are
observed experimentally to have as much as a 106 times
difference in their transport rate constant (18). By modeling
the experimental binding and translocation rates of hydro-
phobic ions, an estimate of the dipole potential is possible.
A number of compounds are known to modify the dipole
potential of bilayers. For example, phloretin is a compound
that appears to reduce the magnitude of the dipole potential,
and it has been studied in planar bilayer systems (19-21).
Previous work carried out in this laboratory demonstrated
that phloretin produces changes in the translocation rates of
hydrophobic ions in lipid vesicles that are consistent with the
magnitude of its molecular dipole moment (22). In these
vesicle systems the membrane concentration ofphloretin can
be easily determined, which makes a quantitative evaluation
of its action possible. In addition to changes in the translo-
cation rates of hydrophobic ions, changes in the dipole po-
tential should also produce changes in the binding of hy-
drophobic ions (10), however, these binding changes have
not been measured.
Recently, several newly developed spin-labeled hydro-
phobic anion probes were described that allow the direct
measurement of hydrophobic anion transport and binding in
vesicle systems (23). These probes provide an excellent com-
plement to previously developed positively charged phos-
phonium probes (24). In the present study, both the trans-
location rates andbinding free energies ofoppositely charged
spin-labeled hydrophobic ion probes (I-III below) are ex-
amined in vesicles. In the context of a simple model, we
demonstrate that these probes provide an estimate of the
membrane dipole potential. In addition, the effects of phlo-
retin and 6-ketocholestanol on both the binding and transport
rates of these probes are examined. Ketocholestanol is an
agent that increases monolayer surface potentials, and it is
expected to increase the membrane dipole potential (25). The
changes produced by these agents on the binding and trans-
port ofhydrophobic ions are shown to be consistent with the
effects of adding the molecular dipole moment of these mol-
ecules to the membrane-solution interface. The use of these
compounds allows the dipole potential to be lowered or
raised over a wide magnitude and provides an excellent ex-
perimental methodology to examine the effects ofdipole po-
tentials on membrane protein structure and binding.
Biophysical Journal
Page 3
Membrane Electrostatics
SCHEMES IV and V.
Phosphatidylcholine (PC) was isolated from fresh hens eggs using the pro-
cedure of Singleton et al. (26) and was stored in a chloroform solution at
-20°C until needed. The spin-labeled phosphoniums I(n) were synthesized
following a procedure described previously except that the appropriate di-
bromoalkanes were coupled to five membered proxyl nitroxides rather than
the six membered TEMPO nitroxide (18). The trinitrophenols II and III
were synthesized using a procedure recently described (22). Phloretin was
obtained from Sigma Chemical Co. (St. Louis, MO), and 6-ketocholestanol
was obtained from Steraloids (Walton, NH).
EPR determination of hydrophobic ion binding
EPR measurements were performedon aBrukerESP300EPR spectrometer,
and the binding of spin-labeled probes was determined as described pre-
viously (24). Briefly, in the presence oflipid vesicles the spin-labeled probes
I-III partition between the membrane and aqueous phases. The resulting
EPR spectrum under these conditions is simply a sum of the spectra pro-
duced by membrane associated and aqueous spins. Because the signal in-
tensities are proportional to the numberofspins in each phase, aquantitative
measure of the resonance intensities can be used to measure the phase par-
titioning of I-III. The ratio of the number of membrane-bound spins to the
number of spins in solution, A, is given by:
Formation of lipid vesicles
Aliquots ofthe egg PC solution were dried by rotary evaporation, and varied
amounts of 6-ketocholestanol or phloretin were incorporated by codissolv-
ing these compounds with the egg PC using benzene/methanol (95:5). The
mixtures were then freeze-dried for a minimum of 15 h at less than 0.5 torr.
Lipid vesicles were formed by dissolving the dried lipid film with
an aqueous solution containing 100 mM NaCl and 25 mM morpholino-
propane sulfonic acid (MOPS), pH 7.0. The suspension was vortexed,
freeze-thawed five times, and extruded 10 times through 0.05-,um Nucle-
opore filters using acommercial unit (Lipex Biomembranes Inc., Vancouver,
BC). The vesicles were centrifuged at 12,000 g to remove nonvesicular lipid.
An analysis ofboth the supernatant and pellet by thin-layer chromatography
showed that the lipid and phloretin (or ketocholestanol) were uniformly
mixed in each fraction. The phospholipid concentration of the final vesicle
suspension was determined using a modified Fiske-Subbarrow phosphate
assay (27).
1 ml of
where A is the baseline to peak amplitude of the high-field nitroxide res-
onance (m = -1) in the lipid suspension,A' is the amplitude ofthis resonance
in the absence ofvesicles, and b/a is a constant that reflects the contributions
that membrane and aqueous spins make to the amplitude of theml= -I
resonance (the values of bla used were 0.04 for probe I and 0 for probes
II and III).
As described previously (18) the phase partitioning, A, of hydrophobic
ions such as I-III is related to the partition coefficient 3, the concentration
of lipid CL, the area/lipid, AL, and the volume/mole of lipid, VL, by the
A plot of A-' versus CL is expected to yield a straight line with a slope of
(3. The values ofAL and VL used here were 66 A2 and 1255 A3, respectively.
The binding constant, K, is determined by dividing the partition coefficient
Franklin and Cafiso
Page 4
Volume 65
by the thickness ofthe binding domain for the hydrophobic ion at the vesicle
surface. For the hydrophobic ion probes used here this distance is taken as
EPR determination of hydrophobic ion currents and rate
The transmembrane currents for I or III were determined by mixing so-
lutions of I or III (final concentrations were approximately 20 ,uM) with the
egg PC vesicle suspensions. This was accomplished using a precision mix-
ing ram (model 715; Update Instrument, Inc., Madison, WI) adapted to a
quartz flat cell in a standard X-band cavity. Upon mixing, a time-dependent
change in the amplitude of the high-field resonance ofprobe I or III occurs
which is a direct consequence of the transmembrane migration of the probe
(28). This decrease in amplitude (increase in membrane binding) is due to
the increase in accessible membrane surface area that accompanies probe
equilibration with the internal vesicle surface. The signal intensityA can be
related to the transmembrane ion current by:
b=Fz[KoVm/Vo []
SO [1 -EO/Eij [dt
where SO is the external vesicle surface area, Ko and K, are the external and
internal probe binding constants, Vmo and Vmj are the external and internal
volumes occupied by the probe, respectively, E.= (1 + KOVmOVo), and
Ei= (1 +KjVmi/Vi). Equation 3 yields a current for the spin-probes I or III
as a function of the time derivative of the high-field resonance amplitude,
dA/dt. If the initial ion current, i,,
change in the signal intensity, (dA/dt), = 0,then the rate constant for inward
movement of the label can be calculated according to the following.
is determined from the initial rate of
Here, [I] is the external aqueous concentration of probe I or III. A detailed
discussion and derivation of these expressions was given previously (28).
Modeling dipole potential changes
The effects of phloretin and 6-ketocholestanol on the binding and transport
properties of hydrophobic ion probes were analyzed by employing a total
potential model for hydrophobic ions similar to one described previously
(10). In this model, point dipoles that generate the intrinsic dipole field of
the membrane are placed on a square lattice at a defined distance from the
bilayer center. The energy of an ion at any point is taken by calculating the
sum of the electrostatic interactions between a charge and the dipole lattice,
AGDipoie, and adding energy terms for the Born-Image energy, AGBOm, as
well as the neutral (or hydrophobic) energy, AGHydrO.An energy profile is
obtained by calculating the energy along a path normal to the bilayer surface.
This model was modified in two ways for the present study. First, binding
and translocation rate data in the absence of phloretin or 6-ketocholestanol
were obtained for the hydrophobic ion probes I-III. This data was combined
with previous data on hydrophobic ions, and the parameters in the model
were optimized as detailed previously to produce the best fit to the exper-
imentally determined values of kf and K (10). As discussed below, this
yielded slightly different but physically reasonable parameters. Second, as
depicted in Fig. 2, the addition ofphloretin or 6-ketocholestanol was treated
as being equivalent to the addition of dipoles to the membrane interface that
were either aligned with or against the intrinsic membrane dipole. This was
modeled by placing an additional layer of point dipoles on a second square
lattice at a frequency corresponding to the membrane-bound concentration
of these compounds (22). Both the magnitude and the position of this ad-
ditional layer of dipoles was allowed to vary. In the case of phloretin, the
dielectric constant of the membrane interior was altered as a function of the
phloretin concentration. Phloretin is known to change the capacitance of
lipid bilayers, a change that could be due either to a change in membrane
dielectric or bilayer thickness (21). Recent 2H NMR work provides no ev-
idence for a change in bilayer thickness in the presence of phloretin (29);
Membrane Solution Interface
Phloretin Dipole
Intrinsic Membrane Dipole <
dipole potential of membranes. When phloretin is added to membranes, it
inserts into the membrane solution interface and is positioned so that a
component of its molecular dipole (approximately 5 D) is aligned against
the intrinsic membrane dipoles. The effect of phloretin on hydrophobic ion
transport and binding is modeled by calculating the electrostatic energy due
to point dipoles on a square lattice at a density corresponding to the mem-
brane concentration of phloretin. The effects of 6-ketocholestanol are mod-
eled in the same manner, except that a component of its molecular dipole
adds to the to the intrinsic membrane dipoles.
Adepiction ofhow phloretin is believed to modify the internal
as a result, the most likely source of this capacitance change is a change in
membrane dielectric. In the modeling carried out here, the dielectric constant
was varied linearly with the addition of phloretin as given by E = 2.0 + x,
where x is the mole fraction of phloretin in the membrane.
This electrostatic model produces a free energy profile for hydrophobic
ions in membranes. To compare the measured translocation rates with the
predictions of this model, the binding constants and rate constants were
calculated from the energy profile in a manner similar to that described
previously (1O). Briefly, the rate constants, kf, were calculated from the
potential energy profiles ofhydrophobic ions by taking the energy difference
between the minima at the membrane interface and center of the membrane
(AGO*). The rate constant, kf, was determined from the expression: kf =
fexp(AGO*/RT), wheref is a universal frequency factor of 6 x 1012 s-I at
room temperature (30). The predicted binding constants, K, were determined
from the free energy profiles by taking the energy difference between the
bulk solution and the minima at the interface (AGO ). This energy difference
is related to the binding constant by the familiar expression K = exp(-AGB/
Phloretin increases the binding of cation probes
and decreases the binding of anion probes
The membrane-aqueous phase partitioning ofprobes I-III to
500-A egg PC vesicles can be determined directly from their
EPR spectra as described under Experimental Procedures.
Shown in Fig. 3 is a plot ofthe ratio ofthe number ofaqueous
to bound spins (1/A) for the hydrophobic anion probe III
plotted versus the reciprocal of the lipid concentration (1/
CL). The slope of this curve is linear over the probe con-
centration shown in Fig. 3 up to a probe:lipid ratio of about
1:100; however, the slope increases at higher probe:lipid ra-
tios. This is consistent with previous data obtained for 44P+
indicating that these hydrophobic ions experience an elec-
trostatic saturation in the lipid bilayer at probe:phospholipid
Biophysical Journal
Page 5
Membrane Electrostatics
the reciprocal of the lipid concentration (1/CL, in cm3 mol-'). The lipid
vesicles were formed by extrusion through 0.05-,M Nucleopore filters in
a buffer containing 100 mM NaCl and 25 mM MOPS, pH 7.0, and the
partitioning of III was measured by EPR spectroscopy using procedures
described previously (22). The solid line represents a fit of Eq. 2 to the data
using a value for the area/lipid, AL, of 66 A2 and a value for VL of 1255 A3.
This fit yields at value for (3 of 22.4 X 10-5 cm.
A plot of the reciprocal of the partitioning (1/A) of III versus
ratios above 1:100 (18). From the linear portion ofthis curve,
the partition coefficients,
I-III, and these values of ,B are shown in Table 1. Inspection
of this data shows that the binding of the negatively charged
trinitrophenol probes II and III is clearly stronger than for
the positive phosphonium I(8), even though the phospho-
nium probe has significantly more hydrophobic character. As
explained above, this difference in binding is a direct con-
sequence ofthe dipole potential. There are strong indications
that the TNP probe III is hydrogen-bonded in the membrane
interface. First, the hydroxyl group on III raises the energy
of binding by about
energy difference is much less than the energy loss that would
have been expected if this probe were not hydrogen-bonded
within the interface. Second, previous work that compared
the motion of the two anion probes (II and III) indicates that
the rotational rate of III in the bilayer is much slower than
that of II, consistent with III forming ahydrogen bond within
the interface (23).
When phloretin is incorporated into the membranes of the
500-A vesicles the binding of both negative and positively
charged hydrophobic ions is altered. Table 1 shows the effect
f3, were determined for probes
1 kcallmol compared to probe II. This
on the partition coefficients of these probes following the
incorporation of5 and 10mol% phloretin into the membrane.
The addition of phloretin produces small but measurable in-
creases in the binding for the positive probe I, and two- to
threefold decreases in the binding of the hydrophobic anion
probes II and III.
Phloretin increases the crossing rate for cations
but reduces the rate for anions
Shown in Fig. 4 is a recording of the high-field resonance of
the nitrophenol probe III when the probe is mixed with phos-
pholipid vesicles.A similar decay in the high-field resonance
amplitude upon mixing with vesicles is seen for phospho-
nium spin labels such as I. These probes rapidly equilibrate
with the external vesicle surface, and this decay was previ-
ously shown to be the result ofthe transmembrane migration
of the hydrophobic ion (28). From a measure of the time
dependence of the EPR signal amplitude, dA4/dt, the hydro-
phobic ion current and translocation rate constant were es-
timated using Eqs. 3 and 4. Shown in Table 1 are the forward
translocation rate constants, kf, for probes I and III in ves-
icles formed from egg PC. The value of kf for III is con-
siderably slower than values obtained previously for the car-
bonylcyanide m-chlorophenylhydrazone (CCCP) anion (22),
and is much slower than the crossing rate forprobe II. In fact,
a value of kf for probe II could not be measured, because its
transmembrane equilibration is faster than can be resolved
with the present instrumentation (<10 ms). This dramatic
difference in crossing rates between III and other hydro-
phobic anions such as II and CCCP is accounted for by the
additional energy that would be required to break a hydrogen
bond, consistent with the idea that III hydrogen bonds within
the membrane interface.
The incorporation of phloretin into the vesicle membrane
alters the transport rates of hydrophobic ions. The transport
rates for probes I and III, as well as previous data obtained
for the CCCP anion in vesicles, are shown in Table
membranes at two phloretin concentrations. The rate of
crossing for the phosphonium I is increased approximately
70-fold upon the addition of 10 mol% phloretin, while the
crossing rates of the anions are decreased by more modest
Any number of physical changes might be expected to
increase the binding of probe I; for example, an increase in
the dielectric constant or disordering of the membrane in-
terface. However, the differential effects of phloretin on the
1 for
Effect of phloretin on hydrophobic ion binding and translocation rate constants
13 x 105 (cm)
kfX 103 (s'1)
6.4 ± 1.0
4.1 ± 1.2
10 103
*Approximate phloretin concentration in mol%.
tData from Ref. 22.
Franklin and Cafiso


text:
This strain came mostly in the form of little minty green Christmas tree-shaped buds that glimmered with fat-headed trichomes. It had a very solid manicure, but there were some darker green leaves visible, along with the dark red and light brown pistils. Perfectly dry and cured just the way we like it, it also had a very well-timed harvest, as it displayed about 25% ambered trichomes and many that were cloudy. A good density for a Sativa-dominant sample, the flower clusters had a little give but were rather solid and left a dusting of trichs on your fingers when handled.
A strong taste of eucalyptus along with some grassy-tasting citrus was the dominant taste profile here. The smoke was fairly thick and tickled the throat, causing a decent amount of coughing, but it didn't have any kind of burning or real harshness. It seemed to taste slightly better through a clean pipe than an IncrediBowl for whatever reason, normally it's the opposite.
(We are NOT doctors – personal opinion ONLY) Motivation, creativity, possible ocular relief, clear-headed relief.
A very productive and energetic daytime strain, this Dutch Treat Haze felt Sativa dominant in profile until the very end, when tiredness and a groggy head kicked in. The almost linear focus on projects and creative pursuits would make this a great choice for a productive weekend morning, but probably not the best for enjoying the company of others, as it was cerebral and slightly introspective. Though it didn't provide a ton of pain relief, it would satisfy most Sativa lovers and those who aren't looking for a strong body high. The pleasant taste and smell make this an above average total package for sure, and it will really please a patient desiring some extra motivation and energy from their meds.
Hybrid, sativa dominant 60/40 - Dutch Treat (80% Indica BC medical strain) x Haze
Tangerine Haze
Original Soma Seeds New York City Diesel x G-13/Haze
Bruce Banner #5
OG Kush x Reservoir Strawberry Diesel (Strawberry Cough x Sour Diesel)
Cougar Piss
SoCal Catpiss x Afghani #1 IBL
The Prophet
Pre-'98 Bubba Kush x Tang Tang males
Isaac Haze
Giesel (ChemDawg D x Massachusetts Super Skunk) x Ace Seeds Bangi Haze (Congo x Nepalese)
Golden Goat (CAM)
Ambrosia (OrganaLabs)
Headband (Karmaceuticals)
Master Kush (Altitude Organic Medicine)
Durban Poison Earwax (Broadway Wellness)


text:
FORT MYERS, Fla. — Boston Red Sox left-hander Jon Lester pitched into the sixth inning against minor leaguers Friday, allowing two run and four hits.
Looking to avoid taking a long bus ride for a chance at facing an AL East rival, Lester remained at the Red Sox spring training complex and struck out seven against Triple-A Rochester, the Minnesota Twins' top affiliate.
"It was good," Lester said. "Got some things done. Got up there in the pitch count, which was good. I was able to do a couple of different things. Threw some changeups behind in the count, so it was good."
The Red Sox lost 1-0 in Dunedin against the Toronto Blue Jays.
Lester threw 100 pitches for the first time this spring in 5 1/3 innings.
Lester has one more scheduled outing this spring, Wednesday. He is lined up to start opening day at the New York Yankees on April 1.
In five Grapefruit League outings this spring, spanning 20 innings, Lester has allowed just two runs for a 0.90 ERA. He's allowed six hits and four walks, and has 16 strikeouts.
Blue Jays 1
Red Sox 0
DUNEDIN, Fla. — Toronto closer Casey Janssen opened in impressive fashion.
Janssen struck out two during a perfect seventh inning in his spring debut as the Blue Jays beat the Red Sox.
"First time out there, I guess it couldn't have gone any better," Janssen said.
Janssen, who finished with 22 saves last season, had surgery in November to address lingering shoulder soreness. After striking out Lyle Overbay and Mauro Gomez, the right-hander got a grounder from Mike Carp.
"His ball was exploding," Toronto manager John Gibbons said. "I thought he was popping the ball pretty good, so that's a great sign."
J.A. Happ continued his strong exhibition season, allowing two hits in six shutout innings for Toronto. The left-hander could begin the season at Triple-A, in a long relief role or at some point move into a starting spot if the Blue Jays decide to drop struggling Ricky Romero from the rotation.
Happ admitted that his undetermined status has weighed on him.
"The great thing is, my teammates are great," Happ said. "That certainly helped."
Happ has given up four runs over 19 innings in six games.
Gibbons again reiterated that Romero is in the rotation.
Romero is 0-1 with a 7.27 ERA in four big league exhibition games. The left-hander had control issues in a minor league game Thursday after working on mechanics with pitching coach Pete Walker.
"He's making some changes, and you don't expect them to happen right away," Gibbons said. "Hopefully his next go around it's going to be better."
Page 2 of 2 - Romero is scheduled to pitch Tuesday against Pittsburgh.
Sergio Santos, expected to fill in if Janssen is not ready for opening day, struck out three, walked one and had two wild pitches during a scoreless eighth.
Boston reliever Alfredo Aceves got the start and scattered two hits over five shutout innings.
The Red Sox lineup consisted of mostly backup candidates and prospects. Overbay, the former Toronto first baseman, went 0 for 2.
"We're going to take every day available to us to make a decision on how our bench is going to round out," Boston manager John Farrell said.
Overbay can opt out of his contract Tuesday.
Boston designated hitter David Ortiz (heel) and shortstop Stephen Drew (concussion) remain out, but have been hitting in a cage at the Red Sox complex in Fort Myers. Both could be on the disabled list when the Red Sox open the regular season April 1 at the New York Yankees.
The Yankees will be without first baseman Mark Teixeira (wrist), third baseman Alex Rodriguez (hip) and outfielder Curtis Granderson (forearm). Shortstop Derek Jeter might also miss the opener due to ankle soreness.
"A lot of temporary situations because of injuries," Farrell said. "I'm sure everybody who pays attention to every opening series, you want to see the marquee players perform. In both cases, we're dealing with some banged-up rosters."
Toronto opening day starter R.A. Dickey threw 102 pitches over eight innings in a minor game on Friday.
"It was a great day for me," Dickey said. "Great in the sense that I threw eight innings and felt fantastic. I got better as the game went on, and felt strong in the later innings."
Toronto infielder Edwin Encarnacion could miss a couple games because of a sore index finger that resulted from an at-bat at the World Baseball Classic.
"It swelled up a little bit," Gibbons said. "The X-ray (Thursday) was fine. No concerns."
Toronto claimed RHP Todd Remond off waivers from Baltimore and optioned him to Triple-A Buffalo. The 27-year-old Remond made his major league debut last season with Cincinnati. To make room on the 40-man roster the Blue Jays put RHP Drew Hutchison on the 60-day disabled list. ... Blue Jays 3B Brett Lawrie (strained rib) is increasing his on-field work, but hasn't resumed batting practice. There is a chance he won't be ready for opening day. "If he had to start the season a few days late, so be it," Gibbons said. "We don't want this thing to creep up again." ... Red Sox prospect Jackie Bradley Jr. had one of the two Boston hits. The outfielder is hitting .429 overall. ... Toronto sent RHPs Claudio Vargas and Ramon Ortiz, and INFs Eugenio Velez, Ryan Schimpf, Jim Negrych, Mike McCoy and Luis Jimenez to its minor league camp.
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