text: My family were on our way home via HWY 50 and passing through Pueblo. I quick looked on tripadvisor for a good place to eat. Romero's was I think #3 on the restaurants and Mexican sounded good. We are glad we stopped, the green chile is amazing- a lot like how my mom makes it! Everything was fresh... read more


text:
Contact Us
Site Map
Good Neighbor Program
Quality of Life
Wolf Pack
Fact Sheets
Freedom of Information
Wolf Pack Heritage
Good Neighbor Program
Wolf Pack Warrior
Editorial Policy
Join the Air Force
Commentary - Wolf Pack SARC tackles sexual assault
Wolf Pack SARC tackles sexual assault
Posted 4/15/2010 Updated 4/15/2010
Email story
Print story
Commentary by Staff Sgt. Nichole Piazzo
8th Fighter Wing Sexual Assault Response Coordinator office
4/15/2010 -
KUNSAN AIR BASE, Republic of Korea
-- April is Sexual Assault Awareness Month. As this month and your year at the Wolf Pack passes here are some statistics to be aware of. An American is sexually assaulted every two and a half minutes. In the Air Force, one in three women and one in eight men are sexual assault victims at some point in their life. More importantly, at least two thirds of sexual assaults are committed by someone who is known to the victim. The majority of victims here at Kunsan have been females between the ages of 18 - 24 and in the rank of E-4 and below.
The Wolf Pack sexual assault response coordinator office has planned many activities throughout the month of April to get the message out. SARC members along with fellow victim advocates will be out in the base lounges, The Loring Club, the Community Activities Center as well as the International Cultural Ville stamping hands with "Got Consent?" every Friday and Saturday. This is a reminder to always get consent regardless of the situation. On the national SAAM day of action, April 20th, there will be two showings of Searching for Angela Shelton and a question and answer session with the SARC regarding the film at 8 a.m. and 3 p.m.
April is also host to Alcohol Awareness Month. The Kunsan SARC office and the Alcohol Drug Abuse Prevention and Treatment office have teamed up to raise awareness of the correlation between sexual assault and alcohol and the significant risk they pose together.
Understanding and avoiding risk can help to reduce the statistics stated at the beginning of the article. Alcohol is the biggest risk. Avoiding or limiting your alcohol use greatly decreases your chance of being a sexual assault victim. Being a good wingman and looking out for each other are great ways to greatly reduce your risk of sexual assault. Communication is also key. Remember to clearly communicate your desires and get consent every time.
The SARC and ADAPT will have a booth at the Base Exchange, April 29th. Wolf Pack members will have a chance to pick up an application to be a victim advocate and to get some free SARC stuff.
Remember, regardless of risk factors, sexual assault or rape is never the victims fault. If you become a victim of sexual assault, you have options for reporting.
Restricted reporting occurs when you can come to the SARC office or a victim advocate and report what happened to you. Victims can get the support they need whether it is medical or mental without anyone in their chain of command knowing about the incident.
Unrestricted reporting is when you report through your chain of command, the SARC office, a victim advocate, security forces, or the office of special investigation. Victims get the support they need and there will be an investigation initiated.
An independent report is when you tell a friend or co-worker and they tell someone in your chain of command. This type of report is automatically unrestricted.
For more information regarding sexual assault and the sexual assault coordinator program visit.
, or the SARC page on the Kunsan SharePoint page. The Kunsan SARC office is located in the fitness center on the second floor within the health and wellness office. The 24 hour SARC hotline is 782-7272.
No comments yet.
Add a comment
Inside Kunsan
View All RSS
The Official Web Site of Kunsan Air Base
Site Map
Contact Us
Security and Privacy notice
Suicide Prevention
Accessibility/Section 508
No FEAR Act

subset: commoncrawl


text:
Man faces fourth DWI chargeJuly 01,2013Man faces fourth
DWI charge
LONDONDERRY A local man is facing his fourth drunken driving charge following a traffic stop Thursday night.
Shortly before 8 p.m., police stopped Raymond W. White Jr., 28, of Londonderry, for what police called numerous motor vehicle infractions as White drove along Route 11.
When police spoke with White, he allegedly gave police a false name.
Police charged White with drunken driving, fourth offense, as well as a charge of providing false information to a police officer. He is scheduled to answer those charges Aug. 13 in Brattleboro criminal court.MORE IN This Just InMONTPELIER — An Amtrak train derailed in Northfield along Bull Run Road around 10:30 a.m. Full StoryWHITE RIVER JUNCTION Judge Nancy Corsones disqualified herself from a bail hearing in a... Full StoryA look at the week back and the week ahead. Full Story
- Most Popular
- Most Emailed


text:
There are no items in your cart at this time.
Footwear etc. Rewards: Enjoy $10 in Rewards Just for Signing Up!
Enter your email address below and we will notify you when this product is back in stock.
A reality of wearing lace-up shoes is that the laces will occasionally need to be replaced. No matter how good the shoelaces are, they simply aren't going to last as long as a sturdy pair of shoes. A set of Replacement Laces can extend the life of a pair of shoes.
Sign up to receive new arrival, sale & special event info from us.
Note: we do not share your personal information with anyone. Period.
"The "walking store" in the mall didn't have my size in the color that I wanted. They were ready to order it, but I thought Footwear etc. might have it. And you did! I don't have to wait two weeks for the store order and you saved me $40!! Thanks Footwear etc...I'll be back!"
©2015 Footwear Etc. All Rights Reserved. For any questions or assistance call 1-800-720-0572.


text:
Maybelline Dream Matte Mousse
3302 reviews
48% would repurchase
Package Quality: 3.3
Price: $$
Package Quality: 3.3
Price: $$
INGREDIENTSCyclopentasiloxane, Dimethicone, Squalane, Dimethicone Crosspolymer, Dimethicone/Vinyl Dimethicone Crosspolymer, Hydrogenated Jojoba Oil, Polymethylsilsesquioxane, Polymethyl Methacrylate, Silica Dimethyl Silylate, Phenoxyethanol, Phenyltrimethicone, Disodium Stearoyl Glutamate, Methylparaben, Vinyl Dimethicone/Methicone Silsesquioxane Crosspolymer, Aluminum Hydroxide, Silica, BHT, Ethylparaben, Butylparaben, Isobutylparaben, Propylparaben, May Contain ( /-):, CI 77891 (Titanium Dioxide), CI 77492, CI 77499, CI 77491 (Iron Oxides), CI 77163 (Bismuth Oxychloride), Mica
on 8/9/2015 9:39:00 AM
Age: 18 & Under
Skin: Combination, Olive, Not Sure
Hair: Blond, Other, Other
Eyes: Blue
I remember using this when I was younger. It was really patchy, orange and didn't cover well. It created a lot of dry patches. I haven't used it in a long time, but I'm afraid of it now so I probably won't repurchase. It was also a really odd texture and was hard to blend.
on 8/5/2015 11:00:00 PM
Age: 30-35
Skin: Oily, Fair, Not Sure
Hair: Black, Other, Other
Eyes: Brown
This is better than the liquid version but I still don't recommend.
on 8/2/2015 7:51:00 PM
Age: 19-24
Skin: Oily, Medium, Not Sure
Hair: Black, Other, Other
Eyes: Black
my current everyday foundation. i have moderately oily t-zone and some dry patches but this glides on smoothly. just moisturize and prime before using and this will blend beautifully. i use the shade medium beige and it's a good shade match. i do get oily but not that much. i just blot an oil sheet. overall a good product. i love the mousse texture more than liquid foundations!
on 8/2/2015 6:48:00 PM
Age: 19-24
Skin: Combination, Fair-Medium, Cool
Hair: Brunette, Wavy, Medium
Eyes: Blue
This really is a love it or hate it kind of thing. I bought it when I was starting off using make up because it was what all of my friends were using. Yes it's thick, very matte and can look cakey if you use too much.. but if you blend it in properly and don't use too much it can actually look ok. It's not the prettiest foundation in the world but I like to use it on days where I think I need a lot of coverage and it definitely has that. I do not use a lot at all. I tend to dip a stippling brush in, not too heavily and then blend it onto my skin. I wouldn't be quick to repurchase but I do kind of like it.
on 7/29/2015 9:43:00 PM
Age: 18 & Under
Skin: Combination, Fair-Medium, Not Sure
Hair: Brunette, Other, Other
Eyes: Brown
I saw another girl my age use this and her skin looked flawless. I have normal/oily skin so I thought it would suit me as well. no it did not! it was orange and clinger to ANY dry patch. didn't blend well. the packaging was nice and it wasn't expensive really and I'm sure it is a really good product, just not for me.
on 7/26/2015 8:09:00 PM
Age: 19-24
Skin: Combination, Fair, Not Sure
Hair: Black, Other, Other
Eyes: Brown
Such a disappointment! A coworker of mine has amazing skin and she claims to use this everyday.. so I had to try it.. for me though, the results were not the same.
Matte to the point of making my skin look dry and lifeless.. and not all matte make ups do this. After a few hours it was still matte but my skin had that ghostly, dead look. Never have I had a foundation that made me look so sickly. I threw it out after just a few uses.
on 7/21/2015 8:37:00 PM
Age: 25-29
Skin: Combination, Fair, Neutral
Hair: Brunette, Straight, Other
Eyes: Brown
Horrible. Orange, streaky, impossible to blend.
I am NC10 w/ yellow undertones. No way would any of these colors ever match me.
Terrible color selection. I don't like the idea of a pot either, dirty and messy.
on 7/19/2015 7:46:00 PM
Age: 19-24
Skin: Combination, Dark, Not Sure
Hair: Brown, Relaxed, Coarse
Eyes: Brown
I love maybelline products in general. I liked this foundation initially because maybelline's caramel is always a great match for me. This gives good coverage and lasts on my normal-dry skin but.....
1. Clings to dry patches on my skin
2. Looks too matte IMO. Like back from the dead ashy matte
3. Hard to apply? It balls up on my skin because of the mousse texture. I blend with a sponge and it gets all over my sink and clothes for some reason. Its even wrose if i try to use a kability brush.
4. Packaging kind of sucks because it's a pot but it's definitely sturdy
All in all I won't buy again.
on 7/18/2015 4:47:00 PM
Age: 18 & Under
Skin: Combination, Fair, Warm
Hair: Brown, Wavy, Medium
Eyes: Brown
I've generally had good experiences with mousse foundations, and this one was just..okay on that scale.
I remember buying Dream Matte Mousse on a whim, and even though I bought shade 2 Light, it still ended up being too dark. I had to wait from about October to a few weeks ago to actually start wearing it. Nonetheless, I was happy to try it out.
Its texture is, of course, that of a mousse; it's a much drier mousse than, say, Covergirl's or Revlon's takes on whipped foundations. I appreciated that, though, because the drying and setting time is nonexistent. It's also very weightless on the skin, so you hardly feel it on you. And if you do decide to feel your face a bit, the finish of this foundation is very smooth and velvety. Also, its color isn't too orange, and it matches well with my skintone.
Now, here are the caveats. I had issues getting a perfectly even distribution of this foundation; there are always spots in which it's streaky, sparse, or too blotchy. Its coverage is pretty light, which doesn't do much for my acne-scarred skin. I need to use more concealer than I would like to admit, and it still doesn't look perfect. Sometimes, it's also difficult to gauge how much of Matte Mousse you even need to use for a perfect application! I haven't found the equilibrium between too much and not enough. Finally; its staying power isn't the best, even with primer.
Those were the issues I had with this foundation; for the most part, though, it's an okay product. It's less than $10 at Walmart, and its packaging is very solid- a recurring, yet pleasing, trait with Maybelline. If you have good skin already, I'd consider giving this a shot. Other than that, Dream Matte Mousse is far from winning the mousse foundations race.
on 7/15/2015 12:46:00 PM
Age: 18 & Under
Skin: Normal, Olive, Neutral
Hair: Brunette, Straight, Medium
Eyes: Hazel
I use the shade Sun beige which is a little darker than my skin tone but I mix it. The product is so lightweight and never looks heavy or cakey. My only downfall is, is the product doesn't stay on for very long which is not what you want, however i did only pay �7... so you get what you pay for.
Popular Foundations
92 Reviews
20 Reviews
240 Reviews
66 Reviews
Video Reviews & Tutorials
MAYBELLINE Dream Matte Mousse foundation review [has Cancer producing ingredient] by LifeThoughtsCamera


text:
Dog quickly takes in, nurses stray kitten in Minn.
JORDAN, Minn. (AP) – A Minnesota woman says she was shocked when her small dog began caring for an abandoned kitten, then started producing milk and nursing the fuzzy gray stray to health.
Pat Weber says the kitten was days old when her grandson found it in the family's barn in early September in Jordan, about 40 miles southwest of Minneapolis.
She says the 2-inch-long kitten was "ice cold." But she felt it move, so she wrapped the kitten in a warm towel. Then it began meowing _ and her 4-year-old Pekingese perked up.
The tawny pooch named Mittens began licking the kitten, who nuzzled in and began suckling. Mittens hadn't had puppies in two years, yet she eventually began producing milk. She's been nursing the kitten ever since.
Weber calls it a miracle.
(Copyright 2012 The Associated Press. All rights reserved. This material may not be published, broadcast, rewritten or redistributed.)


text: Guy vs. Ram in Headbutt Challenge – VIDEO By Steve Peloquin July 16, 2013 1:02 AM Share on Facebook Share on Twitter It's ram against a guy in a football helmet, in this headbutt challenge. Guess who wins?!?!? Category: Sports Buzz Share on Facebook Share on Twitter More Leave a comment Send to a friend! Print this page Pin it! Reddit This! Share on Tumblr! Best of ESPN 1420 Nick Saban Gets Fired Up & Sends Message To The Media – VIDEO Saints Release Phillips & Morgan, Sign Fields & Hills Kourtney Reppert — Babe of the Day Keuchel, Astros Eliminate Yankees, 3-0 Recommended For You Best of the Web


text:
Does your over-the-phone interpretation vendor continue to charge interpretation time after you have completed the call?
Washington, D.C. (Vocus) December 2, 2008 -- In the world of over-the-phone interpretation (OPI), every second counts. In the billing of OPI, a second over one minute can add up over the course of a billing cycle if your vendor uses rounding. When an organization employs an over-the-phone interpretation service, they often negotiate a per-minute price. However, they often do not ask the supplying vendor the question that could save them money. Do you bill your calls to the nearest minute? Nearest thirty seconds? Nearest hundredth of a second? This simple question could save an organization a lot of money by eliminating interpretation time paid for, but never used.
Language Learning Enterprises, Inc. (LLE®), a global, leading provider of 24/7 over-the-phone interpretation services in 150 languages, presents LLE® True Billing as a way for organizations to save money: All over-the-phone interpretation calls are measured to the nearest second. LLE reports that this type of precise billing will present a significant cost savings to customers of every size and volume of usage.
"Conducting business in the language of your customer is vital for client acquisition, retention and satisfaction," says Kathleen K. Diamond, LLE President, Founder and CEO. "In the current challenging economy pay-as-you-go telephone interpretation, translation and language testing services help in this effort. Our LLE® True Billing initiative solidifies our commitment of connecting professional, experienced interpreters to our client and the LEP community at a very affordable price."
"Time truly is money," states Clyde Anderson, Vice President of Marketing for LLE. "Many of our competitors round their over-the-phone interpretatio
|Source: PRWeb
Copyright©2008 Vocus, Inc.
All rights reserved


text:
North America
North America
Recent Activity
Recent Activity
This edited volume rigorously assesses the 1996 U.S. welfare reform law, questions whether its immigrant provisions were ever really necessary, and examines its impact on legal immigrants' ability to integrate into American society.
The impact of climate change as a driver of human migration is expected by many to dwarf all others. Still, certain frequently repeated forecasts of the number of people who stand to be displaced by climate change are not informed by a complete understanding of migration dynamics, as this report explains.
This interactive language access webinar, one in a series offered by the Migration Policy Institute's National Center on Immigrant Integration Policy, examines how New York and Illinois have broken down some of these barriers to proactively engage LEP communities to obtain workforce services.
Migration to the United States from Mexico and Central America's Northern Triangle (El Salvador, Guatemala, and Honduras) has accelerated in the last four decades. This increase has been driven by economic opportunities and facilitated by social networks of friends and family already in the United States.


text:
News Categories
- All News
- Alumni
- Announcements
- Athletics
- College of Arts and Sciences
- Community Events
- Continuing Education
- Distance Education
- Events
- Faculty
- Featured
- Greenville Campus
- Honors College
- International
- ITS Security
- Johnson College of Business
- Library
- Mary Black School of Nursing
- Parents
- Scholars Academy
- School of Education
- Staff
- Students
Featured NewsSubscribe via RSS
Upstate Picked Sixth in A-Sun Preseason Men's Basketball Poll
Macon, Ga. – The USC Upstate men's basketball team has been tabbed to finish in sixth place according to the […]Read More
QEP Salon is Friday, Oct. 9
Join us to hear from QEP faculty and students as they share their experience & practical advice from their work […]Read More
Helping Flood Victims
The Student Government Association and the Office of Service-Learning and Community Engagement, OSCLE, are collecting donations of bottled water to […]Read More
USC Upstate Welcomes Economist Tim Quinlan to Wells Fargo Speaker Series
The George Dean Johnson, Jr. College of Business and Economics at the University of South Carolina Upstate welcomes Tim Quinlan […]Read More
USC Upstate Chemistry Club Presents "Chemistry Colors our World" At Chapman Cultural Center Oct. 17
The University of South Carolina Upstate Chemistry Club will perform a variety of interactive chemical demonstrations to spur appreciation of […]Read More
Wild Earns Fourth A-Sun Player of the Week Recognition
Macon, Ga. – USC Upstate freshman forward Gordon Wild was named the A-Sun Player of the Week for the fourth […]Read More
New Staff Members Join the Spartan Family
Please welcome our four newest staff members to the Spartan Family. Tony Perdomo is the assistant director of counseling services. […]Read More
ITS TIP OF THE WEEK: Don't Get Scammed
Throughout the academic year, ITS will provide the campus with advice, tips and tricks to improve our Internet security. Pay […]Read More
Summer Innovation Grant Recipients Present Their Projects
CETL invites you to join the 2015 Summer Faculty Innovation Grant Recipients for the presentations of their innovative projects, designed […]Read More
Goldberg and Mills Invited to Write About Democratic Education
The Association of American Colleges & Universities invited two University of South Carolina Upstate officials to write for one of […]Read More
Event HighlightsSubscribe via RSS
The Student Government Association and the Office of Service-Learning and Community Engagement, OSCLE, are collecting donations of bottled water to […]
Three legendary innovators will discuss their renowned careers, how innovation has evolved and shaped lives over the years, and where […]
Spartanburg, SC – As the site of a World War I training camp, Camp Wadsworth, Spartanburg played an important role […]
Enjoy beautiful music during Ice Cream Sundays with the Spartanburg Community Band. Concerts start at 7 p.m. each week. Admission […]
Wonder Woman: Celebrating Who You Are! Wednesday, April 29, 2015, 8:00 a.m. – 4:30 p.m. Russell House Ballroom Women's Leadership […]
The Office of Student Life Division of Multicultural Programs will show the film "Dear White People" on Tuesday, March 31 […]
Community EventsSubscribe via RSS
The Student Government Association and the Office of Service-Learning and Community Engagement, OSCLE, are collecting donations of bottled water to […]
A Perfect Victim Documentary Viewing |Oct. 6, 6-8 p.m., Daniel Recital Hall at Converse College Watch the 82-minute documentary that […]
The Scarecrow of the Southeast, Neil Lee Griffin, will perform a free live concert at Chapman Cultural Center this Sunday, […]
SAFE Homes-Rape Crisis Coalition along with the Seventh Circuit Solicitor's Office, Spartanburg County Domestic Violence Coordinating Council, Spartanburg County Department […]
Girls for STEAM will celebrate Ada Lovelace and women trailblazers by learning about the benefits of math; the evolution of […]
FestiFall, the annual history-based festival at Walnut Grove Plantation in Spartanburg, has been cancelled because of the heavy rains that […]


text:
We've known since May that Carbine Studios' MMORPG WildStar will be going to free-to-play this fall. Today, we... Read More
Closed beta for the upcoming free-to-play version of WildStar, Carbine Studios' fantasy MMORPG, went live today.... Read More
Last month, the Sci-Fi MMO, WildStar announced that it was converting to a free-to-play business model. With most... Read More
The days of pay-to-play MMORPGs are coming to an end, and NCSOFT's WildStar is the latest victim. Announced... Read More
Carbine Studios has a plan for WildStar in 2015. The MMORPG originally launched in early June of 2014, it did away with some... Read More
Almost any game can have an exploit, some game developers leave them and others take action against the players that are... Read More
The next WildStar content update will be the biggest one to come to the game yet. It features two new chapters of... Read More
Around 15 days ago we saw the launch of megaservers on WildStar, since then players have come together to join in on... Read More
The launch rush for WildStar is officially over, that means its time to consolidate servers - but how would Carbine go about... Read More
Some of my favorite things in MMOs are the special holiday events that take place. Well, that won't be happening... Read More
WildStar is hands down my favorite game of 2014. With over 10 years of MMORPG experience behind me, I spent the better part... Read More
This is Part Two of our WildStar Review in progress. For Part One, click HERE. This part will cover levels 20 through... Read More
WildStar's content drop #2, titled "Sabotage," is live. And with comes a lengthy list of patch notes. The big... Read More
"Sabotage" The second post-launch content drop for WildStar is on its way. After The Strain-themed update,... Read More


text: I recently stayed at the Royal with my family. Our room was basic but clean and tidy with ample space and storage for the family. We had a balcony which over looked the pool and garden area. The room also had aircon. The rooms could do with a bit of maintenance i.e. there was a hole in the bathroom where... More


text:
Despite producing for Klaxons and Arctic Monkeys, Simian Mobile Disco still had time to make their new album 'Temporary Pleasure'
Saving Playlist
We are saving your playlist, please wait.
Autofill fills your queue, based on the video you're watching
Enter Dj Mode, then click any video to add it to your queue.
Signing In
Signing you out...


text:
|Número de publicación
|Tipo de publicación
|Número de solicitud
|Fecha de publicación
|8 Oct 1991
|Fecha de presentación
|30 May 1989
|Fecha de prioridad
|6 Jun 1988
|También publicado como
|Número de publicación
|Martin F. Finlan
|Cesionario original
|Amersham International Plc
|Exportar cita
|BiBTeX, EndNote, RefMan
|Citas de patentes (7), Otras citas (6), Citada por (103), Clasificaciones (18), Eventos legales (7)
|Enlaces externos: USPTO, Cesión de USPTO, Espacenet
This invention relates to sensors for use in biological, biochemical and chemical testing and in particular to immunosensors used to monitor the interaction of antibodies with their corresponding antigens.
When antibodies are immobilized on a surface, the properties of the surface change when a solution containing a corresponding antigen is brought into contact with the surface to thus allow the antigen to bind with the antibody. In particular, the change in the optical properties of the surface can be monitored with suitable apparatus.
The phenomenon of surface plasmon resonance (SPR) can be used to detect minute changes in the refractive index of the surface as the reaction between the antigen and the antibody proceeds. Surface plasmon resonance is the oscillation of the plasma of free electrons which exists at a metal boundary. These oscillations are affected by the refractive index of the material adjacent the metal surface and it is this that forms the basis of the sensor mechanism. Surface plasmon resonance may be achieved by using the evanescent wave which is generated when a P-polarized light beam is totally internally reflected at the boundary of a medium, e.g. glass, which has a high dielectric constant. A paper describing the technique has been published under the title "Surface plasmon resonance for gas detection and biosensing" by Lieberg, Nylander and Lundstrom in Sensors and Actuators, Vol. 4, page 299. Illustrated in FIG. 1 of the accompanying drawings is a diagram of the equipment described in this paper. A beam 1 of light is applied from a laser source (not shown) onto an internal surface 2 of a glass body 3. A detector (not shown) monitors the internally reflected beam 4. Applied to the external surface 2 of glass body 3 is a thin film 5 of metal, for example gold or silver, and applied to the film 5 is a further thin film 6 of organic material containing antibodies. A sample 7 containing antigen is brought into contact with the antibody film 6 to thus cause a reaction between the antigen and the antibody. If binding occurs the refractive index of the layer 6 will change owing to the increased size of the antibody molecules and this change can be detected and measured using the surface plasmon resonance technique, as will now be explained.
Surface plasmon resonance can be experimentally observed, in the arrangement of FIG. 1, by varying the angle of the incident beam 1 and monitoring the intensity of the internally reflected beam 4. At a certain angle of incidence the parallel component of the light momentum will match with the dispersion for surface plasmons at the opposite surface 8 of the metal film. Provided that the thickness of metal film 5 is chosen correctly there will be an electromagnetic coupling between the glass/metal interface at surface 2 and the metal/antibody interface at surface 8 which results in surface plasmon resonance and thus an attenuation in the reflected beam 4 at that particular angle of incidence. Thus, as the angle of incidence of beam 1 is varied, surface plasmon resonance is observed as a sharp dip in the intensity of the internally reflected beam 4 at a particular angle of incidence. The angle of incidence at which resonance occurs is affected by the refractive index of the material against the metal film 5--i.e. the antibody layer 6--and the angle of incidence corresponding to resonance is thus a direct measure of the state of the reaction between the antibody and the antigen. Increased sensitivity can be obtained by choosing an angle of incidence half way down the reflectance dip curve, where the response is substantially linear, at the beginning of the antibody/antigen reaction, and then maintaining that angle of incidence fixed and observing changes in the intensity of the reflected beam 4 with time.
An object of the present invention seeks to improve on the sensitivity of the basic surface plasmon resonance sensor by utilizing the phenomenon of long range surface plasmon resonance. This phenomenon is described in a paper entitled "Long-range surface-plasmon modes in silver and aluminium films" by Quail, Rako and Simon in Optics Letters, vol. 8, page 377.
The present invention provides a sensor comprising a source of electro-magnetic radiation, a structure comprising a block of material transparent to the radiation from said source, a membrane of dielectric material positioned against a surface of said block, a layer of metallic material applied to that surface of said membrane opposite said block and a layer of sensitive material applied to said metallic layer, means for introducing onto the sensitive layer so as to react therewith a sample to be analyzed, wherein the index of refraction of said sample and/or said sensitive layer is arranged to be equal or nearly equal to that of said membrane, and wherein the index of refraction of the material of said block is higher than that of said membrane and said sample, and means for monitoring radiation from said source which is applied to said transparent block, and which is internally reflected within the structure, the arrangement being such that the radiation causes long range surface plasmon resonance to occur, the characteristics of which resonance, as detected by said monitoring means, are dependent upon the reaction between the sample and the sensitive layer.
When long range surface plasmon resonance is used, the width of the resonance dip is much smaller than that for "normal" or short range surface plasmon resonance--up to ten times smaller. The sensitivity can thus, in principle, be greater.
The transparent block may be made from high refractive index glass or polymer with a refractive index typically of 2. Typically, the refractive index of the first layer and the sample is 1.4. The fact that the block has a higher refractive index than the membrane means that a beam from the source, and incident through the block into said surface will undergo total internal reflection at the interface between the block and the membrane. At resonance, energy is coupled into the metal layer via the membrane and sets up a surface plasmon wave in the metal layer and a corresponding evanescent wave travelling largely in the membrane.
The block may take the shape of a prism or may be a plane rectangular block, or may be semicylindrical or hemispherical as described in our European patent application 0305109. Waveguide optics such as fiber optics could be used, as described in our copending European patent application 89300544.7 filed Jan. 20, 1989 and entitled "Biological Sensors". The invention is also applicable to the optical arrangements described in our copending European patent applications 89304570.8 and 89304572.4 both filed May 5, 1989, and British application 8811919.3 filed May 20, 1988, all entitled "Biological Sensors".
In order to successfully utilize the long range SPR mode, the thickness of the metal layer must be reduced over that for short range SPR, typically to approximately half. For a typical layer of silver, the thickness would be 17 nm.
The thickness of the dielectric layer is critical in obtaining a single narrow resonance dip. If the layer is too thick, multiple resonances occur whereas, if it is too thin a broad dip results. The thickness of the dielectric layer is typically 1200 nm.
Short range surface plasmon resonance results in the generation of a pair of symmetric modes at the metal interfaces on either side of the metal layer. The reduced metal thickness used in the present invention allows these modes to couple together to form a single anti-symmetric mode which has a greatly reduced half-width at resonance. This is known as the long range surface plasmon resonance mode, to distinguish it from short range surface plasmon resonance. In between the two modes, combinations can occur, with both the symmetric and anti-symmetric modes existing together, giving two resonant dips, one for each mode, at difference angles of incidence.
The membrane may be optically coupled to the block by means of a suitable optical coupling fluid or grease. If the refractive index of this intermediate coupling layer is equal to that of the membrane, then this must be taken into account when assessing the thickness of the membrane since, from the point of view of the operation of the device, the intermediate coupling layer forms part of the membrane. References herein to the "membrane" should be interpreted accordingly.
The sensitive layer which is on the opposite side of the metal layer from the incoming radiation is usually very thin, typically 2 nm thick. The thickness of the sensitive layer is augmented during a test by the thickness of the liquid sample, for example serum. The thickness of the liquid sample will generally be much greater than that of the sensitive layer; indeed, in some circumstances the sensitive layer may not exist as a separate entity at all--see below. Typically the thickness of the liquid sample will be about one wavelength of the radiation--in the region of 750 nm. In these circumstances, when assessing the necessary equality of refractive index in the layers on either side of the metallic layer, it is likely that, in the case of the sensitive layer and/or sample, it is the sample which will predominate due to its vastly greater thickness. It is quite possible, however, that the sensitive layer may have sufficient thickness to predominate. In practice, the values of refractive index of the sample and the sensitive layer are likely to be pretty close.
In practice the frequency of the electromagnetic radiation will be within or near the visible region. Lasers are a suitable source of radiation.
Although the layer applied to the metal film is described herein as an antibody layer for use in immunoassays, it will be seen that any sensitive layer whose refractive index changes upon an event occurring can be used thus to provide a sensitive detector having a wide variety of applications in the fields of biology, biochemistry and chemistry. The material comprising the sensitive layer may be specific to a particular entity within the sample or may be non-specific (i.e. may interact with several species of entity within the sample). Examples of specific materials include recognition molecules such as the aforementioned antibodies which will specifically bind an analyte of interest within the sample, DNA/RNA probes which will bind with their complements in the sample liquid, or lectins, glycoproteins or enzyme substrates, all of which are capable of recognizing and binding with the other partner in a bimolecular recognition pair.
Examples of non-specific materials include hydrophobic materials, for example in the form of a monolayer of phospholipid-type molecules to capture amphipathic molecules, or hydrophilic materials which would capture polysaccharides. Indeed, it has been found that the surface of the metal film itself can form an effective non-specific binding material. Silver or gold surfaces will bind proteins or polynucleotides such as DNA or RNA without the need for any further coating and, in this case a separate sensitive layer is effectively dispensed with altogether, and the surface of the metal film itself is used directly for the capture of entities within the sample to be tested.
The metal film material is commonly silver or gold, usually applied by evaporation. The film needs to be as uniform as possible in order to cater for minute movement in the position of incidence of the incoming beam. It is assumed that a structural metal film will give the best resonance and there are various ways in which the membrane can be pretreated to improve the performance of the metal film and in particular to control the natural tendency of such films to form discontinuous islands:
1. Immersion in molten metal nitrates and other molten salts. This has the effect of introducing ions into the surface in a manner which can be structured and which can act as foci for island formation.
2. Ion bombardment to introduce nucleating sites. The removal of the more mobile ions has been demonstrated to reduce the thickness at which the evaporated film becomes continuous.
3. Electroless plating or electroplating over lightly evaporated films (0 to 100 angstroms thick). Electroless plated films survive to a greater thickness than evaporated films and could form more stable nuclei for subsequent coating.
4. Evaporating onto electroless plated films. The electroless plated films have a stronger tendency to form an island structure and to form bigger islands with greater spacing than evaporating films. This could be of advantage in tuning to light of a prescribed wavelength.
Coating performance can also be improved by:
1. Controlling the membrane surface temperature during coating. Using a higher temperature substrate increased the islands' size and the spacing between them and conversley.
2. Evaporating in the presence of a magnetic or electrostatic field or electron emission device to control the ion content of the vapor stream. The state of charge of the substrate is known to affect the island structure.
3. Controlling the angle of incidence of the evaporated vapor stream relative to the membrane surface. The mobility of the evaporated atoms and hence their ability to form bigger islands is greater when the momentum of the atoms relative to the membrane surface is increased.
In order that the invention may be better understood, an embodiment thereof will now be described by way of example only and with reference to the accompanying drawings, in which:
FIG. 1 is a diagram of a known experimental arrangement for demonstrating the surface plasmon resonance effect;
FIG. 2 is a non-scale diagram of the optical layer structure necessary to obtain long range surface plasmon resonance; and
FIG. 3 is a diagram showing a typical apparatus in which the present invention may be utilized.
Referring to FIG. 2, there is shown a diagrammatic sectional view through the sandwich structure needed for long range surface plasmon resonance. Starting from the top in the drawing, the layers comprise a high refractive index glass or polymer block 10 of material transparent to the radiation, a membrane 11 of dielectric material such as p.t.f.e., methyl cellulose or silicone rubber optically coupled to the block 10 by an intermediate coupling layer 12 of coupling fluid or gel, a thin metal layer 13, for example of silver, a sensitive layer 14, for example an antibody layer, and a liquid layer 15 taking the form of a sample, for example serum, to be tested. The latter layer is only present during a test and reacts with the sensitive layer to alter the refractive index of the layer to thus effect the surface plasmon resonance. A radiation source 8 and radiation detector 9 complete the assembly, radiation (probably light) from the source being applied through the transparent block 10 to be internally reflected at the lower surface of the block, the reflected radiation passing out through the block and to the detector 9. As the angle θ1 of incidence of the incident beam of radiation is varied, there will be an angle at which the phenomenon of surface plasmon resonance will be observed, this manifesting itself as a sharp attenuation in the strength of the internally reflected beam. Normal (short range) surface plasmon resonance occurs at an angle θ1 of incidence to the normal of the reflecting metal layer of about 63°. For long range surface plasmon resonance, the corresponding angle is about 50°.
As mentioned above, the use of long range surface plasmon resonance has the potential for much improved sensitivity because the half width of the reflectance dip at resonance can be as much as an order less in magnitude. However, in order to achieve such improvements, the components of the arrangement shown in FIG. 2 must have certain properties:
1. The metal layer 13 should be made thinner in order to provide long range surface plasmon resonance--typically 17 nm thick, as against 56 nm thick for short range surface plasmon resonance. If the metal layer is thick symmetric modes are set upon at both metal interfaces, and extend into the metal as circulatory electron motions. If the metal layer is much thinner, however, these two modes become coupled together to form an anti-symmetric mode, known as long range surface plasmon resonance, providing a very high--Q resonance effect.
2. The layers on either side of the metal layer must be index-matched. In other words, the refractive index of the material of the membrane 11 must be equal or near equal to that of the sample layer 15. Typically, the refractive index of these two layers is about 1.4.
3. The transparent block 10 must be of a material whose refractive index is high compared to that of membrane 11 and layer 15. A typical value is 2.
4. The thickness of the membrane 11 is critical in achieving a single fine resonance, as explained above. This is obtained from Fresnel's equation--see below.
In assessing the exact properties needed, it is convenient to start with the sensitive layer since it is likely that this will have a refractive index which is fixed, depending upon the particular material used. Since the sensitive layer effectively forms part of the sample from the point of view of the surface plasmon resonance, then the sample will conveniently have this same refractive index. This then sets the refractive index of the membrane to be the same as or as near as possible the same as that of the sample. The refractive index of the transparent block 10 needs to be higher than this so that total internal reflection takes place at the interface between the block 10 and the coupling layer 12/membrane 11. A suitable value for block 10 is chosen from available materials. The thickness of the membrane 11 and the metal layer 13 can now be calculated from Fresnel's equation: ##EQU1##
rij =Fresnel reflection coefficient for p-polarized light, as given by: ##EQU2## i=dielectric constant of medium i d=thickness of medium i
θi, θj =angle of wave vector in medium i
ω=angular frequency of incident wave
c=speed of light
The subscripts i, j=1 to 4 refer to the block 10, the membrane 11, the metal layer 13 and the sample 15, respectively. Note, however, that the intermediate coupling layer 12, if present and if of the same refractive index as the membrane will effectively form part of the membrane for the purpose of this calculation. Likewise the sensitive layer will effectively form part of the sample.
As an example a typical antibody layer has an unreacted refractive index of approximately 1.4. This sets the refractive index of the sample and the membrane also at 1.4. The refractive index of the transparent block 10 is set at a convenient value higher than this, say 2. From Fresnel's equation, the following parameters can then be calculated:
Thickness of membrane 11=1200 nm
Thickness of metal layer 13=17 nm
If an intermediate coupling layer 12 of refractive index 1.4 is used, then a typical thickness for this is 200 nm, leaving a net thickness for the membrane 11 of 1000 nm.
It may be desirable to apply to the sensitive layer 14 a protective layer (not shown) of gel or similar material, such as acrylamide gel or hydrogel. During a test, such a gel will permit the antigen within a sample of, say plasma, to travel through to react with a sensitive antibody layer, but will prevent the presence of molecules in the plasma from affecting the result.
There will now be described with reference to FIG. 3, a biosensor suitable for use with the present invention. The apparatus comprises a housing 30 having a hollow interior 31 in which is positioned a printed circuit board 16 on which is mounted the electronic circuitry associated with the apparatus. An aperture is formed in the top part of the housing which aperture is covered by a support plate 17 of transparent material.
A radiation source 18 produces a collimated input beam 19 of electromagnetic radiation. The frequency of the radiation must be such as to result in the generation of surface plasmon waves and in practice will be within or near the visible region. Suitable sources include a helium neon laser or an infrared diode laser, but an ordinary light source, with suitable filters and collimators, could be used.
The light beam 19 is directed at a mirror 20 which in turn directs the light onto a concave reflecting surface 21 and thence to the transparent support plate 17. The mirror 20 is driven by motor means (not shown), to rotate in an oscillatory manner between the limit positions shown by the solid and dotted lines. The result of this is that the light beam applied to the reflecting surface 21 scans backwards and forwards between the positions represented by the beams 22 and 23.
Positioned in the top surface of the support plate 17 is a membrane in the form of a continuous film 24 which is moveable from left to right in FIG. 3 from a supply reel 25 to a take-up reel 26. In its simplest form, the membrane takes the form of a multi-layer structure of the type shown with reference to FIG. 2; in other words comprising a flexible transparent layer, corresponding to block 10, a dielectric layer, corresponding to membrane 11, a metal film layer, for example of silver, and a final layer of sensitive material, such as an antibody layer. The arrangement is such that the layers are in the order--transparent support plate 17--flexible transparent layer--dielectric layer--metal film layer--sensitive layer. Thus the sensitive layer is on the top when seen in FIG. 3.
It will be noted that the flexible transparent layer lies directly against the transparent plate 17, possibly with an optical coupling fluid in between. Preferably the refractive index of the plate 17 is the same as that of the flexible transparent layer so that the two effectively act as a single transparent block, as far as light is concerned. In an alternative embodiment (not shown) the flexible transparent layer in the film 24 is dispensed with, reliance being placed on the support plate 17 to act as the block 10 of FIG. 2. Light incident from reflecting surface 21 behaves in the multi-layer structure in a similar manner to that described above with reference to FIG. 2. The light is internally reflected at a point 27 lying on the interface between the flexible transparent layer and the dielectric layer of the film 24. The internally reflected light passes out of the plate 17 and is reflected off a further concave reflecting surface 28 to be incident on the sensitive surface of a light detector 29.
The reflective surface 21 has a shape which is such as to bring light incident thereon from a range of angles to a single point 27, despite the refraction which inevitably occurs when the light enters the transparent plate 17. Computer analysis of the ray paths can derive a suitable shape for surface 21 to ensure that point 27 is stationary as the scanning between limit positions defined by beams 22, 23 occurs. Likewise, reflective surface 28 has a shape which is such as to bring light incident thereon from a range of angles to a single point at the sensitive area of detector 29.
The reflective surfaces 21, 28 are formed by machining the material, for example aluminium, of the housing 30. If the housing is not fabricated from a suitable material, the reflective surfaces 21, 28 can, of course, be formed as separate elements attached to the housing. Diamond machining of aluminium results in a highly reflective surface whose shape can be tailored, under computer control, to give whatever optical characteristics are required of it.
Provided that the conditions are correct and, in particular, that the angle of incidence of the incoming beam at the interface between the flexible transparent layer and the dielectric layer is correct, then long range surface plasmon resonance will result, causing a sharp dip in the intensity of the internally reflected light as the angle of incidence of the incoming wave is scanned by the mirror 20. A picture of the whole dip can thus be built up by the detector 29 by relating the detector output, on a time basis, with the scanning movement of the mirror 20. This is carried out in the associated electronic circuitry and, since it does not particularly concern the present invention, will not be described further.
The sensitive layer is one whose refractive index changes as it reacts with a sample, in the manner described above. This changes the angle of incidence at which surface plasmon resonance occurs, and thus the reaction of a sample with the sensitive layer can be monitored by observing the dip as the test proceeds. In order to carry out a test, it is simply necessary to place a sample to be tested on top of the sensitive layer in the area of the point 27 at which the light is incident, and observe the changes in the dip characteristics. Upon completion of the test, the film is advanced by a suitable amount, and a further test may then be carried out.
It is preferred to provide the film 24 housed in a disposable cassette 40 which incorporates an opening 41 via which sample may be placed on the exposed film.
|Patente citada
|Fecha de presentación
|Fecha de publicación
|15 Dic 1966
|1 Sep 1970
|Xerox Corp
|Automated chemical analyzer
|2 Nov 1987
|4 Jul 1989
|Stc Plc
|Optical surface plasmon sensor device
|4 Abr 1986
|15 Ago 1989
|Plessey Overseas Limited
|4 Abr 1986
|31 Oct 1989
|Plessey Overseas Limited
|Optical assay: method and apparatus
|4 Jun 1985
|28 Nov 1989
|Universite Paris-Nord
|Means to automatically hold, process, store and analyze biological samples
|4 Abr 1977
|26 May 1981
|Picker Corporation
|Apparatus for performing chemical and biological analysis
|Título no disponible
|"Experimental Observation of the Long-Range Surface-Plasmon Polariton", Craig et al., Optics Letters, vol. 8, No. 7, Jul. 1983, pp. 380-382.
|"Long-Range Surface-Plasmon Modes in Silver and Aluminum Films", Quail et al., Optics Letters, vol. 8, No. 7, Jul. 1983, pp. 377-379.
|"Surface Plasmon Resonance for Gas Detection and Biosensing", Liedberg et al., Sensors and Actuators, 4 (1983), pp. 299-304.
|Experimental Observation of the Long Range Surface Plasmon Polariton , Craig et al., Optics Letters, vol. 8, No. 7, Jul. 1983, pp. 380 382.
|Long Range Surface Plasmon Modes in Silver and Aluminum Films , Quail et al., Optics Letters, vol. 8, No. 7, Jul. 1983, pp. 377 379.
|Surface Plasmon Resonance for Gas Detection and Biosensing , Liedberg et al., Sensors and Actuators, 4 (1983), pp. 299 304.
|Patente citante
|Fecha de presentación
|Fecha de publicación
|28 May 1992
|23 Ago 1994
|Hewlett-Packard Company
|Method and apparatus for detecting the presence and/or concentration of biomolecules
|6 Jul 1993
|7 Mar 1995
|Smithkline Beecham Corporation
|Surface plasmon resonance detector having collector for eluted ligate
|18 May 1993
|29 Ago 1995
|Canon Kabushiki Kaisha
|Measuring apparatus with optically conjugate radiation fulcrum and irradiated area
|12 Sep 1994
|12 Dic 1995
|Eastman Kodak Company
|Production of carriers for surface plasmon resonance
|17 Feb 1994
|26 Dic 1995
|Ares Serono Research & Development Ltd.
|Long range surface plasma resonance immunoassay
|21 Mar 1995
|8 Oct 1996
|Lifescan, Inc.
|Whole blood glucose test strip
|26 Jun 1995
|25 Feb 1997
|American Research Corporation Of Virginia
|Chemical detector employing surface plasmon resonance excited using an optical waveguide configured as an asymmetric waveguide coupler
|13 Mar 1995
|15 Abr 1997
|Regents Of The University Of California
|Method for identifying biochemical and chemical reactions and micromechanical processes using nanomechanical and electronic signal identification
|25 Oct 1996
|29 Sep 1998
|University Of Washington
|Surface plasmon resonance light pipe sensing probe and related interface optics
|25 Oct 1996
|13 Oct 1998
|University Of Washington
|Optical lightpipe sensor based on surface plasmon resonance
|30 Sep 1997
|1 Dic 1998
|Lifescan, Inc.
|Automatic initiation of a time interval for measuring glucose concentration in a sample of whole blood
|7 Nov 1995
|8 Dic 1998
|Clinical Diagnostic Systems
|Production of carriers for surface plasmon resonance
|18 Nov 1996
|8 Dic 1998
|The University Of Toledo
|Sensor using long range surface plasmon resonance with diffraction double-grating
|29 May 1996
|22 Dic 1998
|Kimberly-Clark Worldwide, Inc.
|Piezoelectric resonator chemical sensing device
|25 Oct 1996
|12 Ene 1999
|University Of Washington
|Surface plasmon resonance chemical electrode
|24 Abr 1997
|29 Jun 1999
|Fuji Photo Film Co., Ltd.
|Surface plasmon sensor for multiple channel analysis
|18 Dic 1996
|13 Jul 1999
|Kimberly-Clark Worldwide, Inc.
|Biosensing devices which produce diffraction images
|30 Sep 1996
|21 Sep 1999
|Johnson & Johnson Clinical Diagnostics, Inc.
|Production of carriers for surface plasmon resonance
|25 Oct 1996
|23 Nov 1999
|University Of Washington
|Surface plasmon resonance light pipe sensor
|4 Sep 1996
|1 Feb 2000
|Kimberly-Clark Worldwide, Inc.
|Polymer films having a printed self-assembling monolayer
|18 Dic 1996
|11 Abr 2000
|Kimberly-Clark Worldwide, Inc.
|Method of contact printing on gold coated films
|16 Dic 1997
|9 May 2000
|Kimberly-Clark Worldwide, Inc.
|Optical diffraction biosensor
|21 Mar 1997
|30 Ene 2001
|Kimberly-Clark Worldwide, Inc.
|Gel sensors and method of use thereof
|11 Dic 1998
|24 Abr 2001
|Kimberly-Clark Worldwide, Inc.
|Patterned binding of functionalized microspheres for optical diffraction-based biosensors
|28 May 1999
|31 Jul 2001
|Lifescan, Inc.
|Reflectance measurement of analyte concentration with automatic initiation of timing
|12 Nov 1998
|9 Oct 2001
|Calspan Srl Corporation
|Modular probe for total internal reflection fluorescence spectroscopy
|2 Ago 1999
|15 Ene 2002
|Signature Bioscience, Inc.
|Method and apparatus for detecting molecular binding events
|1 Feb 1999
|9 Abr 2002
|Signature Bioscience, Inc.
|Bio-assay device and test system for detecting molecular binding events
|10 Ene 2000
|23 Abr 2002
|Signature Bioscience, Inc.
|Resonant bio-assay device and test system for detecting molecular binding events
|1 Feb 1999
|28 May 2002
|Signature Bioscience, Inc.
|Computer program and database structure for detecting molecular binding events
|17 Dic 1999
|4 Jun 2002
|Kimberly-Clark Worldwide, Inc.
|Use of wicking agent to eliminate wash steps for optical diffraction-based biosensors
|25 Ago 2000
|9 Jul 2002
|Fuji Photo Film Co., Ltd.
|Surface plasmon sensor for analyzing liquid sample or humid atmosphere
|11 Feb 2000
|20 Ago 2002
|Kimberly-Clark Worldwide, Inc.
|Optical diffraction biosensor
|24 Nov 1999
|1 Oct 2002
|Home Diagnostics, Inc.
|Protective test strip platform
|8 May 2000
|12 Nov 2002
|University Of Washington
|Capillary surface plasmon resonance sensors and multisensors
|28 Feb 2001
|25 Feb 2003
|Home Diagnostics, Inc.
|Method of strip insertion detection
|28 Feb 2001
|1 Abr 2003
|Home Diagnostics, Inc.
|Method for determining concentration of an analyte in a test strip
|17 Dic 1998
|17 Jun 2003
|Kimberly-Clark Worldwide, Inc.
|Patterned deposition of antibody binding protein for optical diffraction-based biosensors
|10 May 2001
|22 Jul 2003
|Fuji Photo Film Co., Ltd.
|Measuring chip for quantitative analysis of substances
|16 Feb 2001
|27 Abr 2004
|Biotell, Inc.
|Optical detection
|30 Abr 2002
|14 Sep 2004
|Agilent Technologies, Inc.
|Reading dry chemical arrays
|24 Jun 2002
|23 Nov 2004
|Lifescan, Inc.
|Reagents test strip with alignment notch
|28 Mar 2002
|26 Abr 2005
|Fuji Photo Film Co., Ltd.
|Measuring apparatus
|26 Jun 2002
|15 Ago 2006
|Kimberly-Clark Worldwide, Inc.
|Enhanced diffraction-based biosensor devices
|11 Dic 2001
|29 Ago 2006
|Kimberly-Clark Worldwide, Inc.
|Methods to view and analyze the results from diffraction-based diagnostics
|11 Dic 2001
|5 Sep 2006
|Kimberly-Clark Worldwide, Inc.
|Systems to view and analyze the results from diffraction-based diagnostics
|13 Feb 2002
|12 Sep 2006
|The Regents Of The University Of California
|Apparatus and method for visually identifying micro-forces with a palette of cantilever array blocks
|3 May 2002
|10 Oct 2006
|Kimberly-Clark Worldwide, Inc.
|Diffraction-based diagnostic devices
|5 Nov 2003
|17 Oct 2006
|E. I. Du Pont De Nemours And Company
|Resonant light scattering microparticle methods
|26 Sep 2002
|30 Ene 2007
|Kimberly-Clark Worldwide, Inc.
|Diffraction-based diagnostic devices
|26 Jul 2004
|6 Mar 2007
|Fuji Photo Film Co., Ltd.
|Measuring apparatus
|3 May 2002
|8 May 2007
|Kimberly-Clark Worldwide, Inc.
|Biomolecule diagnostic devices and method for producing biomolecule diagnostic devices
|3 May 2002
|29 May 2007
|Kimberly-Clark Worldwide, Inc.
|Diffraction-based diagnostic devices
|3 May 2002
|29 May 2007
|Kimberly-Clark Worldwide, Inc.
|Diffraction-based diagnostic devices
|19 Dic 2002
|24 Jul 2007
|Kimberly-Clark Worldwide, Inc.
|Reduction of the hook effect in membrane-based assay devices
|27 Ago 2002
|23 Oct 2007
|Kimberly-Clark Worldwide, Inc.
|Membrane-based assay devices
|18 Jul 2005
|27 Nov 2007
|Bioscale, Inc.
|Method and apparatus for detection of analyte using a flexural plate wave device and magnetic particles
|27 Ago 2002
|1 Ene 2008
|Kimberly-Clark Worldwide, Inc.
|Fluidics-based assay devices
|21 Abr 2003
|18 Mar 2008
|The Regents Of The University Of California
|Composite sensor membrane
|27 Ago 2002
|7 Oct 2008
|Kimberly-Clark Worldwide, Inc.
|Self-calibration system for a magnetic binding assay
|3 May 2002
|3 Feb 2009
|Kimberly-Clark Worldwide, Inc.
|Diffraction-based diagnostic devices
|30 Jun 2004
|21 Abr 2009
|Kimberly-Clark Worldwide, Inc.
|One-step enzymatic and amine detection technique
|2 May 2006
|6 Oct 2009
|Bioscale, Inc.
|Methods and apparatus for detecting cardiac injury markers using an acoustic device
|2 May 2006
|3 Nov 2009
|Bioscale, Inc.
|Method and apparatus for therapeutic drug monitoring using an acoustic device
|2 May 2006
|10 Nov 2009
|Bioscale, Inc.
|Method and apparatus for detecting estradiol and metabolites thereof using an acoustic device
|2 May 2006
|8 Dic 2009
|Bioscale, Inc.
|Methods and apparatus for detecting bacteria using an acoustic device
|2 May 2006
|15 Dic 2009
|Bioscale, Inc.
|Methods and apparatus for detecting viruses using an acoustic device
|1 Nov 2002
|15 Dic 2009
|Kimberly-Clark Worldwide, Inc.
|Membrane-based assay devices that utilize time-resolved fluorescence
|2 May 2006
|19 Ene 2010
|Bioscale, Inc.
|Method and apparatus for detection of analyte using an acoustic device
|25 Abr 2002
|26 Ene 2010
|Kimberly-Clark Worldwide, Inc.
|Polyelectrolytic internal calibration system of a flow-through assay
|20 Jul 2007
|16 Feb 2010
|Kimberly-Clark Worldwide, Inc.
|Reduction of the hook effect in membrane-based assay devices
|22 Oct 2007
|2 Mar 2010
|Kimberly-Clark Worldwide, Inc.
|Membrane-based assay devices
|7 May 2007
|13 Abr 2010
|Kimberly-Clark Worldwide, Inc.
|Biomolecule diagnostic devices
|21 Nov 2003
|11 May 2010
|Kimberly-Clark Worldwide, Inc.
|Method of reducing the sensitivity of assay devices
|19 Dic 2006
|6 Jul 2010
|Bioscale, Inc.
|Method and apparatus for analyzing bioprocess fluids
|3 May 2002
|10 Ago 2010
|Kimberly-Clark Worldwide, Inc.
|Biomolecule diagnostic device
|21 Nov 2003
|24 Ago 2010
|Kimberly-Clark Worldwide, Inc.
|Method for extending the dynamic detection range of assay devices
|22 Dic 2004
|14 Sep 2010
|Kimberly-Clark Worldwide, Inc.
|Optical detection system using electromagnetic radiation to detect presence or quantity of analyte
|22 Dic 2004
|19 Oct 2010
|Kimberly-Clark Worldwide, Inc.
|Electroluminescent illumination source for optical detection systems
|25 May 2006
|9 Nov 2010
|Kimberly-Clark Worldwide, Inc.
|Assay devices that utilize hollow particles
|3 Abr 2003
|14 Dic 2010
|Kimberly-Clark Worldwide, Inc.
|Reduction of the hook effect in assay devices
|28 Feb 2008
|5 Abr 2011
|Iucf-Hyu (Industry-University Cooperation Foundation Hanyang University)
|Long-range surface plasmon optical waveguide sensor
|19 Dic 2003
|17 May 2011
|Kimberly-Clark Worldwide, Inc.
|Laminated assay devices
|21 Nov 2003
|17 May 2011
|Kimberly-Clark Worldwide, Inc.
|Extension of the dynamic detection range of assay devices
|9 Oct 2007
|24 May 2011
|Colorado School Of Mines
|Silicon-compatible surface plasmon optical elements
|21 Abr 2009
|21 Jun 2011
|Kimberly-Clark Worldwide, Inc.
|One-step enzymatic and amine detection technique
|28 Sep 2010
|11 Oct 2011
|Kimberly-Clark Worldwide, Inc.
|Methods of making assay devices utilizing hollow particles
|10 Ago 2010
|7 Feb 2012
|Kimberly-Clark Worldwide, Inc.
|Method for producing biomolecule diagnostic devices
|5 Sep 2008
|15 Ene 2013
|Bioscale, Inc.
|Reusable detection surfaces and methods of using same
|24 Dic 2001
|5 Feb 2013
|Kimberly-Clark Worldwide, Inc.
|Reading device, method, and system for conducting lateral flow assays
|14 Dic 2007
|15 Oct 2013
|Kimberly-Clark Worldwide, Inc.
|Membrane-based lateral flow assay devices that utilize phosphorescent detection
|4 Sep 2013
|22 Abr 2014
|Kimberly-Clark Worldwide, Inc.
|Membrane-based lateral flow assay devices that utilize phosphorescent detection
|8 Jun 2012
|10 Feb 2015
|City University Of Hong Kong
|Optical sensing devices and methods for detecting samples using the same
|21 Abr 2003
|20 May 2004
|Arun Majumdar
|Composite sensor membrane
|5 Nov 2003
|27 Ene 2005
|Prober James M.
|Microparticle-based methods and systems and applications thereof
|25 Nov 2003
|26 May 2005
|Sherrill James V.
|Analyzing biological probes
|25 Jul 2005
|22 Dic 2005
|Prober James M
|Microparticle-based methods and systems and applications thereof
|25 Jul 2005
|22 Dic 2005
|Prober James M
|Microparticle-based methods and systems and applications thereof
|26 Jul 2004
|26 Ene 2006
|Fuji Photo Film Co., Ltd.
|Measuring apparatus
|10 Jun 2004
|3 Ago 2006
|Matsushita Electric Industrial Co., Ltd.
|Biological information measuring apparatus,reference element, and method of using the biological information measuring apparatus
|8 Jun 2012
|12 Dic 2013
|City University Of Hong Kong
|Optical sensing devices and methods for detecting samples using the same
|9 Jun 2005
|9 Jul 2008
|Light waveguide absorption type gas sensor and measuring system
|6 Jul 1994
|19 Ene 1995
|Smithkline Beecham Corp
|Surface plasmon resonance detector having collector for eluted ligate
|Clasificación de EE.UU.
|422/82.05, 422/68.1, 356/318, 436/44, 422/66, 356/445, 422/82.09, 436/805, 435/808
|Clasificación internacional
|Clasificación cooperativa
|Clasificación europea
|30 May 1989
Owner name: AMERSHAM INTERNATIONAL PLC, AMERSHAM PLACE, LITTLE
Free format text: ASSIGNMENT OF ASSIGNORS INTEREST.;ASSIGNOR:FINLAN, MARTIN F.;REEL/FRAME:005086/0937
Effective date: 19890522
|17 Mar 1995
|Fee payment
Year of fee payment: 4
|29 Mar 1999
|Fee payment
Year of fee payment: 8
|16 Oct 2000
|17 Oct 2000
|7 Abr 2003
|Fee payment
Year of fee payment: 12
|10 Abr 2008
Owner name: GE HEALTHCARE BIO-SCIENCES AB, SWEDEN
Free format text: MERGER;ASSIGNOR:BIACORE AB;REEL/FRAME:020783/0192
Effective date: 20071015


text:
Real Estate Management and Development
Company Overview of Atlantic Gulf Communities Corp.
Company Overview
4800 North Federal Hwy
Suite 105-E
Boca Raton, FL 33431
United States
Founded in 1928
80 Employees
Key Executives for Atlantic Gulf Communities Corp.
Atlantic Gulf Communities Corp. does not have any Key Executives recorded.
Similar Private Companies By Industry
|Company Name
|Mosaic Realty Partners, LLC
|United States
|Paris Development Corp., Inc.
|United States
|Roebling Investment Company
|United States
|DRI Development Services, L.L.C.
|United States
|Troutman and Dams, LLC
|United States
Recent Private Companies Transactions
|No transactions available in the past 12 months.
Most Searched Private Companies
|Company Name
|Geographic Region
|Lawyers Committee for Civil Rights Under Law
|United States
|NYC2012, Inc.
|United States
|Bertelsmann AG
|Rush University
|United States
|Citizens Budget Commission
|United States
Sponsored Financial Commentaries
Sponsored Links
To contact Atlantic Gulf Communities Corp., please visit --. Company data is provided by Capital IQ. Please use this form to report any data issues.
Our data partners will research the update request and update the information on this page if necessary. Research and follow-up could take several weeks. If you have questions, you can contact them at firstname.lastname@example.com.


text:
We've put together a special £10 ticket for the home fixture with Hull United to celebrate Non League Day on Saturday 10th October 2015
It may only be September, but the club is already planning for Christmas and we need your help to make our traditional raffle the biggest and best yet
Emley lost a 3-1 lead as they were knocked out of the FA Vase at the second qualifying round by Congleton Town - writes Josh Lindley.
It's set to be a busy weekend of cup football for AFC Emley.
A late mix up between Danny Ryan and Adam Lawlor saw Emley draw at Hallam after being ahead.
Ashley Flynn scored five goals as AFC Emley recorded a historic 10-0 victory over Winterton Rangers.


text:
Find it Fast
5:00 PM - 5:30 PM PTA Meeting
5:30 PM - 6:00 PM Honor Choir Preview
6:00 PM - 7:00 PM 4th Grade Field Trip Meeting
It's time once again to order your Gililland T-shirts and hoodies. Click above for more information. All orders must be paid in cash by October 16.
We need you! Please join the PTA today. Sign-up in the office. Click above for more information.
Our PACE students are collecting new socks for a needy cause. Click here for more information.
See all of the great opportunities available for your kids by viewing flyers electronically through our Peachjar system. Look for the Peachjar icon on our webpage to view flyers or to sign-up. Click title above for more information.
A Family Access account provides parents and guardians with instant access to assignments and grades. An account is free and a great resource for parents and guardians. Click on the link to find out more about this valuable service.
Eagle Mountain-Saginaw ISD 2015-2016 Free & Reduced Meal Applications are available by clicking on the following link: http://emsisd.heartlandapps.com.
Paper applications are available in the Child Nutrition Office, located at 1200 Old Decatur Rd. Building #6 Fort Worth, TX 76179.
The mySchoolBucks program is a easy and convenient way to stay aware of your child's lunch account balance and to remotely add money to an account as needed. Click here to visit the mySchoolBucks website so you can access your child's account.
New website features revitalized design, quick access links, and custom calendar selections.
Check out what your child needs to be prepared for school.
Kids need to cut back on mental exertion, as well as physical exercise, when they're recovering from a concussion, a new study shows.
A guide from the Center of Disease Control about the flu and what to do.


text:
On this episode of Chat with the Experts, we talk with Jason Hodge of Medical Fitness Pros about how to meet your personal fitness goals in 2015 and beyond.
Find Top-Rated Crystal Falls Fitness Centers
Angie's List helps you hire the best - and avoid the rest!
Fitness Centers to Avoid
Top Rated Fitness Centers
Prevent buyer's remorse with us
- Over 3 million people trust Angie's List to help make the right choice
- Be informed to avoid costly mistakes
- Shop with us to ensure a fair price
- Our complaint resolution team will help if a project goes bad
Over 2 million people trust Angie's List.
- Your Membership Includes:
- Instant access to top rated businesses covering 700+ services
- Our Complaint Resolution Team to help when a project goes bad
- On-the-go access to our iPhone, Android, & iPad apps
Access to trusted reviews, the best Fitness Centers and exclusive discounts!
Real Member ReviewsMemberships allow Angie's List to certify that all reviews are real reviews from real members—no fake, bogus, or anonymous reviews here!
DealsAngie's List is a Community that you'll use time after time. Research who to hire, learn tips and tricks, and consult with your neighbors
Instant Community AccessMembers can choose from tons of deals and discounts for projects they need to do, like 30% off exterior home painting!
Complaint ResolutionIf a project goes badly, Angie's List has a complaint resolution team standing by to help you.
Local Articles in Crystal Falls
Weight management starts in your mind so prepare a positive environment in your head to smooth your progress to success with your weight management goals.
Rock climbing, skydiving, archery, parkour running — the possibilities are endless when it comes to trying new physical fitness activities this summer.
Yoga offers many benefits, including lowered stress levels and better sex. Three highly rated experts discuss the benefits and how to get started.
Angie's Answers
Fitness Centers in Crystal Falls, MI
Anytime Fitness - Traverse City
Traverse City
Bianco's Fitness Center
Borgata Fitness Club
Traverse City
Centennial Fitness
Grand Rapids
Chrivia, Dr. Shannon
Curves - Adrian
Dynamic Gym Outfitters
Fit For You Health Club
Traverse City
Grand Traverse Athletic Club
Traverse City
Marquette Soo Bahk Do Academy
MyGym LLC
SpinDoctorplus Exercise Equipment Repair
The Greater Marinette-Menominee YMCA
Twister Fitness
YMCA Dillon Community Center
YMCA Of Marquette County
Join Angie's List to get the best local reviews in Crystal Falls.
- Instant access to reviews for 700+ services
- Exclusive service discounts - up to 70 percent off!
- Top-notch support from our live call center


text:
key standar measurements
sometimes people sew for others, people actually you don´t know (for selling) and you just can´t take the right measurements for a well fitting garment. When you´re using commercial patterns that are already tested, you are not supposed to have any problem with standard proportions, but maybe you´re enrolled in the adventure of drafting your own patterns. In this case, as me, I´ve realized there are few ( super important) measurements I just don´t know where to find p.i.
-bust high
-elbow distance
-shoulder to shoulder width…and go on and on
many available standard size charts are very succinct because is all about guidance, does it exist a FULL chart or procedure to obtain these numbers?
0 Comments
Discussion Categories
Recent Posts
In: PayPal not longer available?
Same problem here. Paypal is the only option for customers from Greece :(
Your website is really cool and this is a great inspiring article.
Thanks for your information, it was really very helpfull..
New web site is looking good. Thanks for the great effort.
Your blog provided us with valuable information to work with. Each & every tips of you…


text:
I'm so excited to finally reveal our living room makeover to you. I partnered with La-Z-Boy to furnish our living room almost exclusively with La-Z-Boy furniture and accessories. The furniture from La-Z-Boy arrived a while ago, but the rug was backordered for several months. My La-Z-Boy in store designer asked me twice if I wanted to chose another rug, but I'm so glad I decided to wait. I love the rug and can honestly say that it was well worth the wait!
Before we get to the tour, I think it's only fair to show you a few pictures of the progression of the living room over the years. About a year after we bought the house in 2007, I painted the walls a lighter sage green and added sconces. We also paid a contractor to double the width of the opening to the kitchen. That made a huge difference. You can see the missing flooring where the small opening used to be below:
The sofa was a hand-me-down from my Godfather's parents.
Underneath the red slipcover was this fabulously reupholstered sofa. Can you tell what year I re-covered it?
Ummm, hello 1990's hunter green plaid. I don't have a photo, but under this plaid monster is the original fabric from the 60's. It was a floral woven tapestry fabric. I still can't believe we sat on this sofa that was over 50 years old. It was hard as a rock.
In 2009, I pulled up the wall-to-wall carpeting and installed antique heart pine wood flooring. I also painted the previously painted white fireplace to look like brick again. You read that right, the brick is painted!
In 2011, I covered the sofa with a new slipcover, but it was still the same uncomfortable hard sofa. We also tried positioning the sofa at an angle in the room.
This past winter we moved the furniture around again and for the first time we felt that we had found an arrangement that worked for us.
If you recall, I took a little field trip to our local La-Z-Boy furniture gallery back in December. I met with Lexie, the designer, who was invaluable with her expertise and design advice. Then you all helped me by voting for your favorite fabric selection (big virtual kisses and hugs from me to you for that!)
The voting was a tie, so I combined fabrics from the two winners and ultimately chose this combination:
Ready to see how they look in the living room? Are you telling me to shut up already and get to the reveal? Your wish is my command, here is our living room reveal: [Read more…]


text: Jamie Squire, Getty Images [ Mike Leach to Possibly Star in New HBO Documentary By Chad Hasty January 16, 2012 Mike Leach on HBO? It could happen. Would you watch? Read More|Comment Category: Opinion Tags: HBO, mike leach Send to a friend! Share on Facebook Share on Twitter Pin it! Reddit This! Share on Tumblr!


text:
#TCPalmSocial review: At Cowboys in Fort Pierce, enjoy great food, but service lacking
Cowboys takes its name seriously: boots are door handles, waitresses are decked out in boots and belts and country music plays on the radio. It's a laid-back, friendly place.
Subscribe to TCPalm.com and our Treasure Coast Newspapers
Ready to subscribe to TCPalm.com, The Stuart News, The St. Lucie News Tribune and The Indian River Press Journal? Choose between a Premium or Digital subscription below.
Need Help? Call us at 1-877-710-6180.
Monday-Friday: 6am-3pm / Saturday: 6:30am-10:30am / Sunday: 7am-11am


text:
Exploring Eckhart Tolle's "A New Earth" Chapter-by-Chapter
In this informative and highly interactive workshop, we will explore Chapter 5 of "A New Earth" and what the teachings of Eckhart Tolle say about "The Pain-Body." See clearly how your emotions and the ego "carry the past" with you today and how the pain-body feeds on your thoughts and drama to keep past pain alive.
Learn how to apply these deep insights and wisdom in your everyday life to feel a greater sense of peace, a deeper acceptance of what is, and a greater alignment with creative intelligence.
The evening is very casual and the group sits in a circle to facilitate deeper connection. The evening begins with an inner-body guided meditation to center the group in presence and to give those who are new to the teachings a powerful experience of their true self. Key insights and spiritual practices are highlighted from each chapter and serve as openings for discussion and sharing.
"It's a good idea to meet with other people who are moving into presence, so if you can find a group of people, who together are not 'trying'... That is very helpful, because the group itself is an energy field; an extremely helpful energy field is created in which it becomes easier for presence to arise." ~ Eckhart Tolle
Greg Larsen has facilitated groups and given talks on the teachings of Eckhart Tolle since 2003 across the United States. He is co-host of Living with Tolle, a #1 podcast on iTunes that explores how to apply the teachings of Eckhart Tolle to daily life.
Workshop: $10
For Info: (310)[masked]
"Thoughtful, deep discussion inspired by Greg. Everyone participated. Very focused and full of sincerity and warmth. The leaders from the Gateway center were so welcoming."
~Nina Hammerstein
"I was really impressed with Greg while I knew I would be beforehand. He knows Tolle's work through and through = an exemplary embodiment, thus a great teacher of it. Very conducive to achieving expansion through the course of the class, and since in a group, even more so."
"I attended one of Greg's talks and feel it was an important and transformative event. I have been a student of all things "spiritual" my entire adult life and especially loved Eckhart Tolle's books "The Power of Now" and the recent "A New Earth". So when I was notified of Greg's talk I instinctively had a good feeling about the event and decided to attend.
When I first walked into the room I was immediately struck with Greg's upbeat, positive and vibrant energy. During the talk Greg dispensed practical and poignant suggestions to keep ourselves anchored in the NOW. At the same time there was a very palpable underlying current of Presence. I definitely felt like I had been swept up in a stream of energy and that feeling lasted well into the next day. I will certainly look forward, while practicing being present, to the next time Greg comes to NYC.
I would highly recommend that if he comes to your area that you take advantage of being in the presence of someone who is practicing being in the Present in a very big way."
~Michele Desmoulins
I started the group because there wasn't any other type of group like this. I've met some great folks in the group who have become close friends and have also met some amazing business owners.
— Bill, started New York City Gay Craft Beer Lovers
Meetup members, Log in
By clicking "Sign up" or "Sign up using Facebook", you confirm that you accept our Terms of Service & Privacy Policy


text:
We pay for the shipping back to our warehouse if you are not fully satisfied. Our Free Return Shipping Offer is unmatched industry wide and a true testament to the confidence in the quality of our furniture. No Re-Stocking Fees, No-Hidden Charges.
The Vogue 5ft. high bar console table unites stainless steel and solid teak into a uniquely modern outdoor design, though it is suitable just as well indoors. With a capacity to seat up to 6 with the combination of the Vogue Stacking Bar Stool, this bar table has a sense of refinement with an understanding of functional requirements. Plastic glides allow the table to be placed on any surface, indoors or outdoors.
To view all the bar sets containing this Teak and Stainless Bar Table, please click below:
View other Westminster Bar Furniture here:
Teak Barstools | Teak Bar Tables |
Teak Bar Sets
As you browse through our pages and speak with our knowledgeable customer representatives, you will find there are quite a few reasons why many have returned and recommended others to shop with us over the past 12 years. Here are some that we often hear why many of our customers think we stand out:
Yes,everything arrived fine
So far I see your products as very "high end" (good quality) which is the image I am looking for
C C , Parkland, FL
Assembly Service Notice
Assembly is included with this item when this option is selected. With the assembly option selected, orders that are $1500 or over will also include White Glove Service. White Glove Service, in addition to assembly, includes unpacking, placement of furniture as well as removal and disposal of all debris including cartons and wood crating material. In stock cushions and in stock umbrella fabrics will be included with Assembly/White Glove Services. Made to order cushions and made to order umbrella fabrics are shipped separately via UPS and are not included with Assembly/White Glove Services.
Please allow 2-4 weeks for delivery when choosing Assembly/White Glove Services. Standard, non-assembled, delivery is 2-10 days.
Teak Treatment Service Notice


text:
Brenda james (1000 galleries found) mixed videos HD videos pictures
Doggy Style Standing Rolled Up Skirt Fuck With Brenda James
05:10, 58961 views, 4 days
Watch Brenda James video
Sexpov With Brenda James
13:24, 53984 views, 4 days
Watch Brenda James video
Filthy Light Haired Milf Brenda James Swallows Big Dick In 69 St
04:50, 59015 views, 6 days
Watch Brenda James video
Busty Blond Sweetie Brenda James Rides Big Cock Of Kinky Ramon N
04:50, 50421 views, 4 days
Watch Brenda James video
Stacked Fair Haired Mom Brenda James Gets Doggy Banged Rough
04:50, 62606 views, 5 days
Watch Brenda James video
Brenda James is the wife good..
15, 6499, 5 years
Brenda James is the wife good naked at the kitchen
Two milfs Brenda James and..
00:29, 3429, 6 years
Brenda James and Raquel Devine share one dick
Sexy milf Brenda James
00:29, 2100, 6 years
Brenda James is a naughty milf who likes to suck two cocks at once
Two milfs Brenda James and..
00:29, 1878, 6 years
Horny cougars Brenda James and Raquel Devine.
Busty milf Brenda James
00:29, 1868, 6 years
Brenda James is a juggy cougar ready to suck any cock she is offered
Brenda James is a naughty..
16, 4169, 5 years
Nasty professor Brenda James having fun with student's cock.
Brenda James
00:30, 2623, 4 years
Brenda James' likes playing host, so when her son's friends drop by while..
Passionate date of lesbians is..
03:01, 3747, 4 years
All natural things which happen between lezzies Brenda James and Zoe..
Cum lust doxy Brenda James..
05:00, 2994, 4 years
Cum lust doxy Brenda James deserves the Nice shot of jizz after a hot fuck
Breasty slut Brenda James gets..
05:00, 2933, 4 years
Breasty slut Brenda James gets fucked nice and hard from her sexy back
Stripped girlfriend Brenda..
05:00, 3920, 4 years
Stripped girlfriend Brenda James fills her youthful Mouth with a..
Screwed Momma Brenda James..
04:53, 12461, 4 years
Screwed Momma Brenda James getting plowed hard on her sugary pleasing love..
Fucking Momma Brenda James..
05:00, 3663, 4 years
Fucking Momma Brenda James feels the unbending 10-Pounder screwing her..
Scorching sexy Brenda James..
05:00, 32405, 4 years
Scorching sexy Brenda James wants for a scorching actionion with her young..
Scorching hawt MomMa BrenDa..
03:00, 2176, 4 years
Scorching hawt MomMa BrenDa James acquires that bald twat cracked Deep by..
Brenda James constricted milf..
03:00, 4247, 3 years
Brenda James constricted milf Booty fucked deep and dirty before facial
Dirty Milf slut Brenda James..
03:00, 3953, 4 years
Dirty Milf slut Brenda James slamming dripping twat on massive cock
Sexy Milf Brenda James..
02:01, 14984, 3 years
Massive glory hole fuck cock.Her first big cock hardcore.
Sexy milf Brenda James gets..
8, 6584, 3 years
Sexy milf girls fucking video.
Brenda James dreams of..
8, 3603, 3 years
Free sex video with big fat cock.Men with big fat cocks on tubes.


text:
The Blood Moon is rising today, and this lunar eclipse, like Earth's changing season, is an example of how the universe can perfectly align. Let us embrace this powerful moment to create practices and set intentions. [Read more...]
The Central Hub of the Patheos Pagan Portal
The Blood Moon is rising today, and this lunar eclipse, like Earth's changing season, is an example of how the universe can perfectly align. Let us embrace this powerful moment to create practices and set intentions. [Read more...]
Honoring the dísir (female ancestors) is an important part of modern heathen ritual. What do we know about ancient heathen women, and how can we apply that knowledge to our practice today? [Read more...]
For years, I heard the Christian expression to 'lean on God' in times of struggle, strife, and turmoil. But for many, and for centuries, that God isn't or wasn't an option. What if there was another option? What if we leaned on Earth and instead of the cup of salvation that Christianity offers, we sought to nurture the healing vessel of a Earth Mother? [Read more...]
When I speak of love, it is not abstract, and it is not romantic or romanticized. It is action that is holy. [Read more...]
It's funny–whenever my practice begins to falter, whenever I get tied up in how to please this deity or how to work that spell, it's science that helps me center myself. The Tree of Life and the breathing Earth. The race of electrons and the swirl of galaxies. These things are my declaration. My Shema. [Read more...]
Tantra is the magic of transformation. It is the magic of bringing self into alignment and aligning our hearts with the Goddess as both the Immanent love that flows in, among, and around us, and also the transcendent exalted from which all blessings flow. [Read more...]
Death kindly seems to be stopping everywhere I look these days, in the faces of those whom I adore and care for, and in the hearts and minds of many within the world: death of loved ones, death of relationships, and even the death of nations. Perhaps Earth herself? Whether by choice or happenstance, death is stopping to remind us of Her part in the ebb and flow. Do you resist her invitation? How does the person practicing alone grow from the experience of death? [Read more...]
Copyright 2008-2015, Patheos. All rights reserved. Terms of Service | Patheos Privacy Policy | Website Development By Patheos Labs


text:
Topics also related to Top News:
Volkswagen board in crisis talks
WOLFSBURG, Germany - Volkswagen's supervisory board was holding crisis talks on Wednesday, facing deadlines from German regulators and U.S lawmakers to explain its rigging of diesel emissions tests and what it is doing to tackle the scandal.
Recent Top News
DNA research deployed in war on cancer scoops Nobel Prize
STOCKHOLM/LONDON - Three scientists from Sweden, the United States and Turkey won the 2015 Nobel Prize for Chemistry on Wednesday for working out how cells repair damaged DNA, providing new ammunition in the war on cancer.
AB InBev makes $104 billion bid for rival brewer SABMiller
BRUSSELS - Anheuser-Busch InBev , the world's largest brewer, launched an improved bid for SABMiller on Wednesday, offering just over 68 billion pounds ($104 billion) for its largest rival to extend its reach into Africa and other markets.
Syrian army and Russian jets target rebels in western Syria
BEIRUT - The Syrian army and allied militia carried out ground attacks on insurgent positions in Syria on Wednesday backed by Russian air strikes, in what appeared to be their first major coordinated assault since Moscow intervened last week, a monitor said.
Ex-U.N. General Assembly head, five others face U.S. bribery case
NEW YORK - U.S. authorities charged a former president of the United Nations General Assembly, a billionaire Macau real estate developer and four others on Tuesday for engaging in a wide-ranging corruption scheme. | Video
Iran's supreme leader bans negotiations with the United States
DUBAI - Iran's Supreme Leader Ayatollah Ali Khamenei on Wednesday banned any further negotiations between Iran and the United States, putting the brakes on moderates hoping to end Iran's isolation after reaching a nuclear deal with world powers in July.
Fifteen dead as South Carolina gripped by historic flooding
- South Carolina grappled with the damage wrought by record rainfall, as the death toll from widespread flooding rose to 15 on Tuesday and residents braced for more evacuations in areas near dams and swollen waterways across the state. | Video
Ex-Yemen president's party says accepts peace terms
SANAA - The party of Yemen's former president, a main player in the messy, months-old civil war, said in an emailed statement that it accepts a peace plan brokered by the United Nations in talks in Oman.


text:
100 Black Men Of The Philadelphia Region Inc
Rating: 0 stars 0 0 review 126
Nonprofit Issues:
Children & Youth
PO Box 613 Oaks PA 19456 USA
Filter Reviews by Role
Promote This Nonprofit
GreatNonprofits badges allow you to raise awareness of your favorite nonprofits on your own web sites!
GreatNonprofits News
See What The Most Quotable Nonprofit Leaders are Saying
Reviews for 100 Black Men Of The Philadelphia Region Inc
Get discovered. Invite people to share their experiences about this nonprofit!


text:
Product Experts Available Now!
Free Shipping on Orders Over $99
Filter by Type of King Pin
$929.95 - $964.95
$907.25 - $1,630.90
Sidewinder Fifth Wheel King Pin with Rotating Turret by 5th Airborne, 16K
Code: ST400-SW16K
Retail: $1,329.39
Price: $907.25
Sidewinder is the premium fifth wheel pin box coupler designed specifically for short-bed pickups. Its worry-free turning design allows you to make up to 90-degree turns without exiting the truck, throwing a switch or even pushing a button!
5th Airborne Premium Fifth Wheel Air-Ride Coupler - Extended Pin Box - Leland 7920 Trailer Frames
Code: 5AB-E7920
Retail: $1,407.58
Price: $964.95
5th Airborne Premium 5th Wheel Air Ride King Pin w/ Sidewinder and Turret - 16,000 lbs
Code: ST400-5AS16K
Retail: $2,295.40
Price: $1,630.90
5th Airborne sidewinder replaces your 5th-wheel trailer's king pin and alters the pivot point of your rig, enabling you to make up to 90-degree turns. Enclosed air spring in pin box absorbs shock and adjusts for a smooth ride.
Sidewinder Fifth Wheel King Pin with Rotating Turret by 5th Airborne, 19K
Code: ST400-SW19K
Retail: $1,446.69
Price: $1,032.28
Trailair Air Ride 5th Wheel King Pin - Leland 7920 - 21,000 lbs
Code: UF35-946208
Retail: $1,299.00
Price: $929.95
Experience smooth, stable towing with your fifth-wheel trailer by replacing your ordinary pin box with an air-ride-equipped model. The scissoring action of the coupler allows the interior air bag to absorb road shock so that you won't feel it.
Trailair Air Ride 5th Wheel King Pin - Leland 7910 - 21,000 lbs
Code: UF35-946209
Price: $939.95
Filter Products
Contact and Help
What our customers are saying:
Great price and very prompt service.
Fredericksburg, VA
Filter Results
Order Information
Customer Service
Stay Connected
Subscribe to email newsletter
privacy - we don't send unsolicited email
All images, layout and content copyright etrailer.com


text:
Recent news and updates
Every time I attend a Giving Voice conference, gathering, retreat, share a phone call or get together with a few GV sisters, I always leave refreshed, renewed, energized and excited. This year's National Conference in Kansas City from August 6-9 was no exception.
In Western New York we do no start school until after Labor Day. Grateful that Labor Day is late this year I sit at my desk skimming through my lessons from last year. A sigh escapes as I think; do I have enough energy, patience, and love to embrace this school year? Do I have anything to offer these inner city middle school girls? Their reality is so different from the way I grew up.
Before, during and after the conference, we had a blog going so many others could join in-check it out at http://giving-voice.weebly.com There you will see reflections, poems and songs that our sisters felt inspired to share about the conference.


text:
Well, México is out.
A following chat and ride with between me and Frenchie, and she cures the sad face.
Here is what I wore to the last game when Argentina got lucky and won... je jeee.
Will you watch any of the remaining games? Is your favorite team still playing? or....UNFAIRLY disqualified :D
Do tell. And if you did some bike+WorldCup+AM action, my friend KT from VeloVogue has the poolphotonitis, and has made a new super fun group for you to submit the fever photos, make sure you check it out: Bike To The Cup
Thursday, July 1, 2010
El TRI, love.
Posted by m e l i g r o s a at 7/01/2010
Labels: events in SF 2010, fun, la blogesphere, ride on, the frenchie
5 comments:
Subscribe to: Post Comments (Atom)


text:
Re: [linux-dell-laptops] Re: Fedora 4 on Dell XPS M140
Expand Messages
- Oh good grief - consider that I have 10 Mandriva, 7 Fedora and one
Ubuntu machine at last count (and countless Windoze machines).
parenthetically_yours wrote:
>Nope there's usually a separate boot, swap and the rest is a LVMWhat I mean is that by default everything of importance is in /.
Therefore, when you want to re-install the system and have to format /,
your home dir is also formatted and all your data is wiped. It is very
easy to create a /home during installation and resizing partitions later
is easy too. Then you can re-install without having to reformat /home.
So, just be careful during the install of FC - don't just blindly accept
all defaults.
>I suspect for most users encrypting the disk will be more trouble than it's worth.Encrypting the /swap and /home partitions is very easy with Mandriva.
The system asks you for your passphrase at startup and after that it is
transparant. See this: http://www.aerospacesoftware.com/raid-howto.html
and scroll down to the heading Encrypted File Systems.
It works on servers too. If a server would reboot due to a power
failure, then the passphrase request will timeout. You can then later
log in via SSH and mount /home manually, at which point the system will
ask for the passphrase.
The Dell password system is useless - a 10 year old can rip the disk
drive from a machine and read it on another...
>The gist of it was that the small performanceSorry, it is a performance hit and what is that about difficulty with
>improvement would cost lots of hassle in finding compatible pre-built
>software and would cause hassle for people that build software.
finding software? My Mandriva 2006 mirror occupies almost 30GB. That
is the opposite problem of having a shortage. Anyhoo, source code is
processor independent.
BTW, all my Fedora machines are running standard kernels downloaded from
kernel.org and compiled for i586. That happened due to a SSL problem in
FC4. I had to re-install and recompile a shedload of stuff to make FC4
work. Overall, I am extremely unimpressed with FC4 - it cost me a hell
of a lot of time and money, but the client wanted it...
- Herman
I hope we're on the same side here - pro linux on Dell laptops. To be
honest I have nothing against Mandriva, but I find your anti Fedora
comments unhelpful.
> Oh good grief - consider that I have 10 Mandriva, 7 Fedora and oneFine - I would have thought that gave you a more balanced viewpoint.
> Ubuntu machine at last count (and countless Windoze machines).
You suggest disk encryption, which will cost a 2% performance hit
(according to the link you gave). It will also make it harder to reuse
the home partition with other installations of linux.
You recommend using i586 optimised binaries on an i686 which will give
you what performance improvement over typical i386 code? I'm guessing
you'd struggle to get 2% without fiddling the benchmarks. Try harder,
read the kernel docs and go pester your distro for i686 binaries :)
Now bear in mind that most processes are IO-bound (rather than CPU
bound). I'll take my 2% performance boost by having unencrypted disk
access, thanks.
Finally, you say the Dell HDD password is useless, I disagree, you'll
find that it's cheaper to replace the HDD in a stolen notebook than
send it off to get the HDD password reset. Yes it's possible to
circumvent but the disk won't just work in another machine, and the
average 10yo won't be much help. It also protects the whole disk,
including the windows partitions, with no overheads (unlike software
encryption of specific partitions).
(ref: http://www.pwcrack.com/harddisk.shtml,
>Finally, you say the Dell HDD password is useless, I disagree, you'llYou can't replace the HDD in a stolen notebook - the machine is gone -
>find that it's cheaper to replace the HDD in a stolen notebook than
>send it off to get the HDD password reset. Yes it's possible to
>circumvent but the disk won't just work in another machine, and the
>average 10yo won't be much help. It also protects the whole disk,
>including the windows partitions, with no overheads (unlike software
>encryption of specific partitions).
you don't have it anymore. Unless you are the asshat whole stole the
notebook... ;-)
The purpose of encryption is to prevent the person who stole the
machine, from reading your accounting and other sensitive data.
Mandriva uses AES encryption. Without the passphrase, a thief cannot
access your data. The only thing the thief can do, is format and
re-install. (I had an IBM Stinkpad stolen and got an $8000 credit card
bill a couple of months later - since then, I'm using encryption
The Dell BIOS password doesn't help in this regard, since it is trivial
to circumvent. As I mentioned, simply insert the disk drive in a
desktop machine and read it. The password may be good protection
against your little kid sister who doesn't know how to use a screw
driver, but it sure won't help against my teenage son... :)
- Herman
Not sure if you're aware of the Dell passwords. There are three, user,
admin and a separate HDD password.
The HDD password does pretty much what you describe, and more. It
prevents the disk from being used outside the machine. IT WON'T WORK
IN A DESKTOP WITHOUT THE PASSWORD. A thief can't even reformat and
reinstall which is why I say it's cheaper to replace the locked disk
than read it. The links I posted give more details.
You mention being defrauded after losing a thinkpad - out of interest,
why did you feel the need to store information that could let you be
defrauded in the first place? If you knew you had lost such
information surely you'd have to do the same things you'd do for a
lost wallet (cancel cards etc.)?
- parenthetically_yours wrote ..
> prevents the disk from being used outside the machine. IT WON'T WORKOK, wasn't aware that Dell now use those kind of disks. However, all you need to do to recover the data, is replace the controller card. If a perp has hardware access to the disk drive, then only encryption can keep the data safe.
Only the paranoid will survive... :-)
> why did you feel the need to store information that could let you beI run Quickbooks on my notebook, since I work all over the place, this is handy. Older versions of Quickbooks work on CxOffice.
> defrauded in the first place?
However, I think what the perp accessed was a scan of a credit card statement, which was still on the desktop. Fortunately, the damage to me was only $50, thanks to state law.
Your message has been successfully submitted and would be delivered to recipients shortly.