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The ever popular Crafty Vintage Weekenders returns for 2015. Enjoy a family fun day out with a quirky twist.
The ever popular Crafty Vintage Weekenders returns for 2015. Enjoy a family fun day out with a quirky twist.
Preston Garden Services Ltd provide high quality gardening and landscaping services throughout Preston,Lancashire and the North West....
Featured in Garden Services in Preston
Smart Image Workwear is home to Preston's largest selection of Workwear....
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Bangla Fusion - Your Local Indian Restaurant,Preston where you will find the finest Indian food, prepared and served by attentive staff in a charming authentic Indian setting....
Featured in Indian Restaurants in Preston
Reeders Dance, Preston dancing school offering dance tuition in Preston for over 20 years....
Featured in Dance Schools and Classes in Preston
Salvatore's is on of the best Italian Restaurants in Preston, blending traditional and contemporary twists to create truly unique takes on classic Italian dishes....
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Undoubtedly the best and most successful networking group in Preston who meet every Friday at The Marriott Hotel, Broughton just off junction 31 of the M6....
Featured in Network Marketing in Preston
If you are looking for a unique picture for your home or as a present then you should call Preston's leading canvas printing company, Canvaspoint.
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Salvatore's is on of the best Italian Restaurants in Preston, blending traditional and contemporary twists to create truly unique takes on classic Italian dishes....
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From shopfitting to wooden flooring and flooring services from craftsmen joiners, MLS Joinery Services of Preston should be your first choice as a Preston joiner and a carpentry fitter of Kitchens, flooring, staircases and tricky, specialist jobs....
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Holistic therapy, or complementary alternative therapy as it is sometimes called is recognised by the medical profession for it's effectiveness in physical and mental pain relief....
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Meryl R said about: Daniel John Hair Lounge
After reading about Daniel John Hair Lounge online, I made my first visit today. I was very impressed with the help and advice for changing my hairstyle and the excellent cutting and I'm very happy with my new hairstyle....
Added February 2015
mandy h said about: Daniel John Hair Lounge
Always tries to fit you in, they provide a 5 star service, always makes you feel important, great sense of humour, the salon peaceful, relaxing and has great coffee too. And to top this off great stylist , who takes to their to get to know you, your needs and never fails to impress.
Added May 2013
Yasmin P said about: A1 Self Storage
Gary at A1 provides excellent service,I would highly recommend to anybody needing storage.
Added February 2013
With the horse meat scandal still under the spotlight, CHARLOTTE WAREING talks to one Preston farmer about his meat supply (Source -LEP)
Preston's historic train station is to get a facelift.
Lancashire Evening Post have teamed up with Northern Rail to bring you an amazing rail offer.
1,000-1 moon landing bet success (Source - LEP)
A rapid pace does not bother you in the least right now. Time moves quickly when you use it well, and you have got the current pattern down to a science. As far as you are concerned, there are no boundaries when it comes to life. An open door is an invitation for you to come hurtling through. Dont spend any of your precious time worrying about smooth landings today, Aries. The Air and Fire that surround you will help keep your feet from touching the ground.
You know what you want, and you even think you have figured out a way to get it. Once you decide to go after something, you are not about to let anyone prod you into doing anything you dont want to do. Any type of commitment makes you nervous at the moment. When the Moon is in its current Sign, the Bull insists on taking life one glorious moment at a time. You like to think of yourself as being on the cutting edge, but to others it looks more like the middle of the road.
It does not take you long to figure out the plan. Logic is your best friend in the world when the Moon is in its current position. Ideas come and go as easily as breathing right now. If you were a car, you would be something extremely fast, yet also fuel-efficient. There is no limit to what your onboard computer can handle, especially when you know what commands to give it. When mingling with others, dont be surprised if they dont quite get your point. Your IQ is among the highest in the room.
You know there is something that you are supposed to be doing, but you can not quite remember all of the details. Check your calendar again, and then ignore it. Not all good things happen on schedule, Cancer. You are growing stronger and more positive by the minute, which means you dont have to have your hand held through the whole process. Soon this dreary illusion will be unable to contain your new brightness, but until then, you will have to have a little faith that it will happen.
You have got your hands in every pot, Leo, and you would not want it any other way. Once you get involved, you want to stay that way. The Lion and his or her current project were made for each other. Whether or not you get rich from this, you will gain experience that might be even more valuable in the long run. This is the perfect time to let your friends in on your investment and/or management secrets. What good is knowledge if you cant spread it around?
You are not about to budge until you get to say what you came here to say. Virgo pays a price for sticking to the subject, but you are determined not to let it break you. Dont be surprised when the restless abandon you. Your captive audience ridicules you, but, as far as you are concerned, the show must go on. If you must play the canary, be sure that you are still singing when you leave this coal mine. Applause would be nice, but do not expect it.
The great mood you are in today is not much fun unless it is shared with the people you care about. Go ahead and play with others like you did when you were a child, Libra. Bring the game outdoors. No matter what activity you decide to take part in, you and your friends need room to move around. Shouting an intimate secret at the top of your lungs will make it seem even more true. Air Signs are unstoppable for most of the day.
You may be feeling that you are the exception to every rule. The more you understand a law, the more tempted you are to break it. Your conscious has apparently taken a holiday, so this is a good time to do the opposite of what your instincts tell you to do. Lock your charge cards in a drawer, and leave the room before you spend your life savings. Take time to relax and read a good book; a quiet evening is what you need.
This can be one of the easiest things you have ever done, or the most difficult -- it is all up to you. You and only you can decide whether both sides will end up polarized or merged. A superficial approach is far from perfect, but it is better than none at all. If this whole process fails to interest you, you are probably asleep. Of course, sometimes it is not the activity that is boring you, but the company you are in. This is a good time to make friends with interesting people.
You have given it your absolute all, but now you are ready to quit. And, as frustrating as it is, you still have to listen to people who stopped providing useful information a long time ago. As long as your decision is reasonable, you should get all of the necessary support. Try to avoid people who appear flighty or distracted. In order to rebuild the foundation, you will need solid players on the team. If you are not up to being the captain, start recruiting your replacement now.
You only know how to go about the day one way, and that is with all of your effort focused on your goals. It is great to have such positive energy, but keep in mind that your full-thrust intensity is too much for people with delicate systems. If possible, it is a good idea to save your best for the other Air Signs in your circle of acquaintances. You may have your best ideas in the morning, but think tanks and playgroups thrive for the whole day.
The worst of it may be over, but you are still feeling the residual hurt of a bad situation or someone who did you wrong. Treat yourself royally when you feel self-pity kicking in. For now, choose what you want to do and leave the rest for someone else. A challenging personal or work-related project can wait until you are better equipped to face it. As far as everyone else is concerned, the credit of your recent success is enough to cover the occasional failure.
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Jon Boydon, who plays Tommy DeVito in the West End musical 'Jersey Boys', and his wife Nicky Willson, who until recently was starring in Mamma Mia, visited the BMI Syon Clinic this week to experience the latest in cutting edge 4D ultrasound technology.
Jon and Nicky, who is 27 weeks pregnant, are expecting their first child and had been invited by the Syon Clinic to come face to face with their unborn baby with the latest 4D ultrasound technology. The 4D refers to the technologies ability to allow Jon and Nicky to view their unborn baby in the womb with 'real time' moving 3D images. Jon and Nicky were able to see their baby in clear detail moving in similar ways to that of a newborn baby. The technology uses a similar level of intensity to that of a 2D ultrasound, yet the 4D scan opens up a more intimate way for expecting couples to experience the joy of bonding with their unborn baby. After the scan Jon and Nicky were able to take away a gift pack, containing a DVD of the moving images from the scan, framed photograph, teddy bear and baby clothes, to commemorate the moment.
After the scan Jon commented: "Syon Clinic is a fantastic high-end facility that has both friendly and welcoming nurses and clinical staff. The technology being used to produce a 3D, real-time moving image of our unborn baby is truly amazing and has really helped us get a sense of what they will look like when we welcome them into the world. The traditional 2D scans don't provide anywhere near the level of clarity that the 4D scans do. Also being able to take away the DVD is great as we can share the experience with both our family and friends. Being able to see our baby's face is the best Christmas present ever and I would recommend it to any expectant parents."
The 4D scan at Syon Clinic is preformed by an experienced sonographer who is also able to determine the sex of the baby and ensure that their development is normal and that the baby is healthy. Jenny Liggitt, the General Manager of BMI Syon Clinic commented: "The 4D scanning technology isn't a replacement for the 12 week dating scan or 20 week anomaly scan so we always recommend to our patients that they keep all their NHS antenatal and ultrasound appointments. The 4D scan is however an excellent complement to these and really helps a couple bond with their baby and build up their excitement for the big day. It was an absolute please inviting Jon and Nicky down to Syon Clinic. Having seen Jersey Boys and Mamma Mia in the West End it was a great pleasure for all our staff to meet them both and from everyone at Syon Clinic we would like to wish them and all our patients a very merry Christmas."

subset: commoncrawl


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Coaching Staff on Penman: I honestly believe Brittnee is going to be a very pleasant surprise as an all-arounder. She saw action as a bar specialist, but we are going to see her in the all-around this year.
2001--Utah State: Competed as a bar specialist for Utah State... Finished in the top three on bars in six meets, including taking top honors against Southern Utah (2/2/01) and BYU (3/9/01)... Recorded a season-best 9.900 on the bars, earning a second-place finish against Boise State (1/26/01)... Season bar average of 9.602.
Club/High School: An elite gymnast for Mark Lee, Jeff Eaton, Shelly Schaerrer and Nicole Trewitt at Rocky Mountain Gymnastics... Placed 25th in the all-around at the 1995 USA Championships... Competed in the American Classic Championships from 1994-96... Participated in the Level 10 regionals in 1996... Did not compete during her junior and senior seasons.
Personal: Full name is Brittnee Ann Penman... Pre-medical major... Career plans include becoming a cariologist or an orthopedic surgeon... Daughter of Darren Penman and Michelle Dowda... Enjoys camping, spending time with family and friends and golfing... Chose Utah State because of the coaches and the pre-med program... Birthday is Sept. 23, 1980.
Collegiate Bests
Bars 9.900 (2) NCAA Regional (4/6/01)
Social Media
USU Athletics Official Twitter Feed:
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Utah State Gymnastics Staff...
Utah State's gymnastics staff earned two regional awards following a stellar season that ended...04/09/2015
Utah State Gymnastics Ends ...
Utah State gymnastics (11-15, 2-7 MRGC) concluded its season with a fifth-place finish at the...04/04/2015
Utah State Set To Compete...
Utah State gymnastics is set to compete at the Berkeley Regional Championship, hosted by...04/03/2015
MEET NOTES: Utah State...
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Utah State gymnastics will host its annual girls gymnastics camp this summer, running Sunday,...03/30/2015
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Utah State gymnastics (10-11, 2-7 MRGC) will compete as the fifth seed at the NCAA Regional...03/23/2015
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Utah State gymnastics (10-11, 2-7 MRGC) finished its regular season at the Mountain Rim...03/21/2015
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Utah State gymnastics is set to wrap-up the 2015 regular season at the Mountain Rim Gymnastics...03/20/2015
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Event Archives
Prayers during a time of grief
My Grandpa Don passed away yesterday. We were very close, and I am still in shock about it. So hard to believe.
McKinney in Taiwan
President and Professor of American Religion Bill McKinney will present a public lecture at Yu-shan Theological Seminary in Hualien, Taiwan.
McKinney in South Korea
President and Professor of American Religion Bill McKinney will present a public lecture at Yonsei University in Seoul, South Korea.
Liew to to present in Indianapolis
PSR professor Tat-siong Benny Liew will present at the Wabash Center Consultations meeting in Indianapolis.
Hispanic Heritage Month Chapel with PSR Community Dancers
Linda Jaramillo, preaching
Executive Minister of the UCC's Justice and Witness Ministries
Jose Ramos & Patricia Wood, convening
Community Wide Media Shout Training - Part 1
Learning to Use Technology in Worship
PSR Board of Trustees Meeting
The PSR board of trustees will be on campus Monday, October 19 and Tuesday, October 20. Please greet them!
Singer/Activist Holly Near to receive CLGS Leading Voice Award
The Center for Lesbian and Gay Studies in Religion and Ministry, will be presenting its Leading Voice Award to singer and activist Holly Near on Sunday, October 18, during the 11 AM service at New Spi
McKinney to preach in Sedona
President Bill McKinney will preach at the Church of the Red Rocks in Sedona, AZ at 8:30 am and 10:30 am.
Congregational Leadership
PSR's Certificate of Ministry Studies program will offer this lay theological education workshop Saturday, October 17, from 9am to 2pm in the Mudd Building on PSR's campus.


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|Reviews for Drabbles from Stanford
| DLillith21 chapter 1 . 6/21/2012
Love Stanford era stories with Sam and Jess, love those insights into what he might have been like back then. Hope you have more coming.
| Patricia de Lioncourt chapter 1 . 5/31/2012
Loved these drabbles! The one with Dean and the soldier was sweet, and I loled at the Harry Potter one.
| Tisha Wyman chapter 1 . 5/31/2012
I loved that he bought a jacket. It still would have been easier on Dean if Sam would have just called him once in a while. I guess Sam did not realize just how much Dean needed him


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Leigh Ann Henion and her boyfriend were madly in love—but he couldn't escape the mysterious legacy of his family's dark past.
20 (More) Secrets Men Keep
Men love gadgets, and they also love soda. What better way to combine their two loves than with the Sodastream "Genesis" kit? This soda maker turns natural water into sparkling without batteries or electricity. And with its sleek, mod design, it'll look great in the kitchen, next to your gadgets. $120 at Bloomingdale's.
You know that travel is no reason to downgrade the quality of your toiletries. But does he? When he easily gets this satchel past airport security and uses Anthony Logistics' products — like the foaming face wash and high-performance moisturizer — he most definitely will. $70 at Anthony.


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An army of moms
Published December 22, 2012 1:01 am
This is an archived article that was published on sltrib.com in 2012, and information in the article may be outdated. It is provided only for personal research purposes and may not be reprinted.
When drunken driving was killing hundreds of schoolchildren every year, a group of American moms started Mothers Against Drunk Driving.
During the 32 years since then, drunken driving deaths have slowly decreased. Today, the thoughts, views and culture of driving while intoxicated has changed the three-martini lunch is a relic.
We can similarly lower the numbers of gun-related deaths through sensible ownership laws, regulations and a different view of gun ownership.
President Barack Obama will not take away your semiautomatic assault rifles, but an army of suburban soccer moms over time could.
Joe Spencer
Salt Lake City
Reader comments on sltrib.com are the opinions of the writer, not The Salt Lake Tribune. We will delete comments containing obscenities, personal attacks and inappropriate or offensive remarks. Flagrant or repeat violators will be banned. If you see an objectionable comment, please alert us by clicking the arrow on the upper right side of the comment and selecting "Flag comment as inappropriate". If you've recently registered with Disqus or aren't seeing your comments immediately, you may need to verify your email address. To do so, visit disqus.com/account.comments powered by Disqus
See more about comments here.
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NPR Newscast
4min 45sec
by Frank Deford
March 25, 2009 Four big-name players regarded as locker room poison were banished by their teams, only to be given another chance. But will the recession change the attitude toward highly paid malcontents? Commentator Frank Deford says even announcers don't quite get how much players' self-aggrandizing behavior irks fans.
<iframe src="http://www.npr.org/player/embed/102303350/102322791" width="100%" height="290" frameborder="0" scrolling="no">
March 18, 2009 Since 1995, both Tennessee and Connecticut have won five NCAA championships apiece — so that all the other women's teams in the country have split a measly four between them. And this year, Connecticut will almost surely win again.
<iframe src="http://www.npr.org/player/embed/102020112/102036444" width="100%" height="290" frameborder="0" scrolling="no">
March 11, 2009 It's the tired expression used to describe the teams that just might win a berth in the 65-team NCAA men's basketball tournament. Commentator Frank Deford says it's time for the sports media to come up with new language, especially at a time when so many jobs, houses and lives are "on the bubble."
<iframe src="http://www.npr.org/player/embed/101683016/101689599" width="100%" height="290" frameborder="0" scrolling="no">
March 4, 2009 Baseball fans may romanticize about how there is no clock in that sport, but basketball was saved by the 24-second clock. And while the sports that have clocks all use them differently, one thing is the same: It's bad for the game to sit on the clock.
<iframe src="http://www.npr.org/player/embed/101393661/101408101" width="100%" height="290" frameborder="0" scrolling="no">
February 25, 2009 At 6 foot 8, basketball star LeBron James isn't exceptionally tall by NBA standards. But commentator Frank Deford says James has an unusual combination of power and agility that makes him a standout in his sport.
<iframe src="http://www.npr.org/player/embed/101116282/101133353" width="100%" height="290" frameborder="0" scrolling="no">
February 18, 2009 The signs and the manifold rumors that Yankees infielder Alex Rodriguez used performance-enhancing drugs were there all along. Commentator Frank Deford says that in a very real way, what has happened to Rodriguez isn't all that bad for baseball.
<iframe src="http://www.npr.org/player/embed/100789366/100804806" width="100%" height="290" frameborder="0" scrolling="no">
February 11, 2009 As Valentine's Day approaches, the one place where a little love is most needed is in the National Football League. Football is a brutal game, and commentator Frank Deford says it's time for everyone involved in the sport to address the health needs of retired players.
<iframe src="http://www.npr.org/player/embed/100539758/100553889" width="100%" height="290" frameborder="0" scrolling="no">
February 4, 2009 At some point, the PGA tour became a wholly subsumed subsidiary of Tiger Woods, and without him there simply doesn't appear to be any there there. The PGA: Professional Golfers Anonymous.
<iframe src="http://www.npr.org/player/embed/100220218/100229992" width="100%" height="290" frameborder="0" scrolling="no">
January 28, 2009 The Pittsburgh Steelers and Arizona Cardinals meet this Sunday in the Super Bowl. Commentator Frank Deford says the annual football championship has become a fixture on the American calendar, an unofficial but widely observed holiday marked by ritual and excess.
<iframe src="http://www.npr.org/player/embed/99917741/99942848" width="100%" height="290" frameborder="0" scrolling="no">
January 14, 2009 Former NBA star Charles Barkley is taking a leave of absence from his television work after his arrest for suspected drunken driving. Commentator Frank Deford says Barkley's career has been marked by his outsized talent and behavior — but his critics should calm down a bit.
<iframe src="http://www.npr.org/player/embed/99285683/99328462" width="100%" height="290" frameborder="0" scrolling="no">
January 7, 2009 The Florida Gators and the Oklahoma Sooners meet Thursday in Miami for the national college football championship. But the game won't likely settle the controversy over how college football chooses its national champion. And that's just one of the oddities of the sport.
<iframe src="http://www.npr.org/player/embed/99055694/99071853" width="100%" height="290" frameborder="0" scrolling="no">
December 31, 2008 By asking a struggling New York City for an additional $259 million in tax-free bonds to help finance a new stadium (while signing free agents for well over $400 million), the Bronx Bombers present themselves as the very model of arrogance and let-'em-eat-cakeness.
<iframe src="http://www.npr.org/player/embed/98830935/98861526" width="100%" height="290" frameborder="0" scrolling="no">
December 24, 2008 John Mackey had a distinguished career with the Baltimore Colts. He now has dementia, and his wife, Sylvia, has stood by him and urged the league to help other athletes with dementia. Commentator Frank Deford says football is an intense team game — but it's Sylvia Mackey who is an outstanding teammate.
<iframe src="http://www.npr.org/player/embed/98668764/98674613" width="100%" height="290" frameborder="0" scrolling="no">
December 17, 2008 Commentator Frank Deford agrees with politicians calling for a college football playoff to determine the national champion. But it means letting go of a long-held belief: that sports and politics shouldn't mix.
<iframe src="http://www.npr.org/player/embed/98336093/98369783" width="100%" height="290" frameborder="0" scrolling="no">
December 10, 2008 You don't have to read the box scores to know that statistics are a big deal in sports. But lately, commentator Frank Deford has noticed a glut of meaningless sports statistics.
<iframe src="http://www.npr.org/player/embed/98016313/98068852" width="100%" height="290" frameborder="0" scrolling="no">
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Recent: Ad Age Data Conference
Rakuten Marketing Blog
Dana Pylayeva, a 16 year IT veteran and Manager of Software Engineering, at Rakuten Marketing recently presented an engaging and dynamic workshop at Agile 2015!
Attribution is still a difficult topic for many companies to discuss. There isn't a 'one-size-fits-all' model out there, which can cause unsettling discussions and tough decisions for any business looking to adopt attribution.
Did you know 33% of Instagram users open the app when they first wake up? Last Thursday morning, after opening up our own feed, we attended the Advertising Week Instagram presentation and panel led by Daniel Habashi, the Director of Brand Development. His presentation highlighted the announcement of the newly opened ad platform and showcased the business capabilities of the app.
This month's advertiser tip is brought to you by Linda Mandolfo, Director of Client Services, NY.
Looking to gain more exposure in the affiliate space? Want to target a new customer demographic? This article will explore the best ways to give your brand the exposure it needs to start generating the sales you want!
Starting this month, you will have access to RAN's New Reporting Platform (beta). With this new platform comes lots of exciting enhancements, not least of which is that data returns significantly faster!
Don't let lost commission haunt you this Halloween. Maximize your earnings this season by posting offers from some of Rakuten Affiliate Network's top advertisers today. Click the link below to access spooky savings, frightening finds and more!
Register for this month's Campus Events to learn more about being a successful advertiser or publisher in the Rakuten Affiliate Network. You can find more information about our events in the Advertiser Help Center or Publisher Help Center, including featured speakers and a longer description.
Over the past four years, Rakuten Affiliate Network has been ranked the top affiliate network by mThink for providing quality service and support to our growing network of advertisers and publishers. With your help this year, we are working to earn the #1 Affiliate Network title for the unprecedented fifth year in a row.
Q. Tell me about Belstaff.
A. Belstaff is a global luxury lifestyle brand steeped in its unique British heritage and the spirit of adventure. In Belstaff, the fearless explorer and fashion enthusiast alike can discover an easy yet refined luxury for a modern lifestyle influenced by Belstaff's rich history and storied archives.


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This Pearstone Gold Series 2 RCA Male to 2 RCA Male Audio Cable is a dual audio cable that is ideal for left and right audio applications. Pure OFC (oxygen-free copper) is used to provide sharp and crisp sound. Encasing the OFC wiring is a rugged SuperFlex PVC outer jacket that is both durable and flexible. The corrosion-resistant connectors feature a positive-grip strain-relief shell, 24k split-pin contacts, and 24k gold-plated terminations. Ideal for a wide range of audio applications.
|Cable Type
|Dual RCA audio interconnect
|6' (1.8 m)
|Dual braided copper and aluminum mylar; over 95%
|Connector 1
|2x RCA male
|Connector 2
|2x RCA male
|Packaging Info
|Package Weight
|0.75 lb
|Box Dimensions (LxWxH)
|8.347 x 6.85 x 0.709"


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Most people have had a leg cramp at least once in their lives, and some may even wince at the thought of a charley horse. However, expecting moms should be aware that these painful, radiating muscle spasms may be commonplace during pregnancy and are typically nothing to worry about.
The good news is that this symptom usually does not occur during the first trimester. It tends to begin in the second trimester when your belly is just starting to protrude. As you continue to gain pregnancy weight, you may notice that leg cramps happen more frequently. While they can happen all throughout the day, they are most noticeable at night, and might even interfere with getting good quality sleep. Some moms-to-be might even report that it adds to their insomnia.
Keep your legs moving
If you're worried, there are some things you can do to prevent this unpleasant sensation. For instance, not sitting or standing with your legs crossed can help. Instead, whenever you can remember, try to focus on rotating your ankles and wiggling your toes while you sit, as this increases the circulation in your legs.
Another good prevention method is to stretch. Even if you take just five minutes every couple of hours to stretch your calves, you'll be less likely to experience a spasm. In fact, if you do experience a leg cramp, stretching the foot heel-first while flexing the toes back toward the shins can reduce the discomfort.
Also, if you have some time to spare, go for a walk! Besides preventing leg cramps, physical activity is great for you and can help you avoid gaining excess weight during pregnancy. Just make sure that you wear proper-fitting footwear, especially if your feet have been a bit swollen - another common symptom of pregnancy.
Provide your body with its daily nutritional needs
Dehydration tends to have some sneaky side effects, and leg cramping is one of them. This is why you should try to drink as much water as you can throughout the day. Even if you sneak in a glass of juice in there, it can be beneficial.
In addition, it's important to faithfully take your prenatal vitamins as your doctor instructed you to. Some evidence suggests that magnesium and calcium deficiencies may make an expecting mom more likely to get leg cramps than pregnant women who have adequate levels of the nutrients.
If you experience a leg cramp and the pain persists, you should call your healthcare provider. Although it's rare, the pain that accompanies these spasms may be the sign of a more serious issue, such as a blood clot, in which case, you don't want to do anything that may make it bigger or allow it to travel.
Did you experience leg cramps during your pregnancy? What helped prevent and get rid of them? Let our readers know by leaving your answers in the comments section!


text: The rooms were comfortable & clean, the location is convenient. The weekend we were there the hotel was overrun by several school age teams....definitely not what I enjoy but the staff was good about keeping things in control. The maintenance man came to our room to fix the tv remote, he was very polite & even asked if any of the kids had been too noisy by our room. There was also a massage therapist offering chair massages by the fireplace in the lobby for a reasonable price & I really enjoyed taking advantage of that!


text: The staff was very friendly, sometimes extremely attentive and other times had to wait for drink refills and check. I ordered the surf and turf, the steak was excellent and the lobster very good, the asparagus was cold. I had breakfast a couple of times while at the hotel, an order plus the... read more


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Rockfax Description1) 5+, 2) 5+, 3) 6a, 4) 6a. The right-hand line of bolts on the main section of the face gives a sustained climb that can be done in three or four pitches. Watch the rope drag on the spectacular final pitch through the capping roofs. © ROCKFAX
Hidden - AltLd - 03/Mar/10
Not sure which pitches we joined up, there were only 4.Adam Potter - AltLd O/S - 26/Feb/09 with Andy Yule
Hidden - AltLd O/S - Apr/07
Hidden - Lead O/S - Apr/07
Hidden - AltLd O/S - 27/Feb/07


text: In Pictures: One by Two Trailer Launch! Movies, News, Videos | December 22, 2013 | | by Stacey Yount EmailFacebookTwitterPinterest Check out these cool pictures of the trailer launch event of the movie One By Two starring Abhay Deol, Preeti Desai. The movie is directed by Devika Bhagat and it's scheduled to release on 31st Jan 2014. Check out the trailer too! EmailFacebookTwitterPinterest


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ESPN Portable Instant Table Tennis Set with Retractable Net
$20.00 Our Price
$79.99 Retail
Armed with this ESPN Portable Instant table tennis set, you'll be able to turn any 6' wide or smaller tabletop or elevated flat surface into a table tennis court for fun competition anytime and anywhere. It sets up in less than a minute, and with the included pair of paddles and 3 balls, you and your opponent will be trading serves and volleys just like that. Table tennis is a fun game for one and all, and here's your opportunity to have your own set that's easy to take anywhere. Want to know more? • Portable table tennis table set • Net extends up to 6' in width • Adjustable posts • Sets up in minutes • Please allow up to 7 business days for item to ship What's in the box? • Expandable/retractable net with posts • 2 paddles • 3 balls • Carrying bag
Today's Deals
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Thanks to a variety of antiretroviral medications available, especially the widely used HAART combination therapy, those who contracted HIV/AIDS from the 1990s on and have been able to maintain drug compliance, are aging longer with the disease, something that is recognized with National HIV/AIDS and Aging Awareness Day each year on Sept. 18. Continue reading
Tag Archives: Aging
Navigating the 'geriverse' whether it's your normal beat or not
Aging. We all do it; some better than others.
Why do some people keep going strong into their 90s and beyond, while others become frail, infirm, or lose cognitive ability while still a "young-old?" To report on aging is to open a Pandora's box of related issues, from care delivery to policy matters; insurance, finance, housing, nutrition, family relationships, technology … you name it and chances are there's a story angle on aging. Continue reading
A physician offers his view on the White House Conference on Aging
Bruce Chernof, M.D., is a geriatrician, president of The SCAN Foundation, based in Long Beach, Calif., and a former chairman of the Federal Commission on Long Term Care. He was in the audience for last week's White House Conference on Aging, listening from multiple perspectives. Overall, he said in a phone interview, he was pleased with the outcomes.
Q: What were your overall impressions of the conference?
A: It's important the President was there and spoke up as forcefully as he did. We needed the President to take part in this conversation. It was a very interesting and different conference but I liked the underlying theme: how do we discuss and transform aging? Everyone likes to talk about the scary stuff first – the diseases, the falls, the dementia. There's not enough focus on the positive aspects of aging, and that limits our ability to focus on everything older adults can and do contribute. Continue reading
Ms. Seegert goes to Washington
When the confirmation email arrived in my inbox late Friday afternoon, I had all but given up on obtaining press credentials to cover the once-a-decade White House Conference on Aging on Monday, just three days away.
But there it was: "You have been confirmed …"
After dashing off a few quick emails to Pia and Len, searching for an affordable, decent hotel (not as easy as it sounds) and making Amtrak reservations, it hit me – "holy cow, I'm going to the White House on Monday! Continue reading
Older same-sex couples gain financial protection in SCOTUS decision
In the wake of last month's Supreme Court ruling on marriage, same-sex married couples in all 50 states should now qualify for financial protection against impoverishment under Medicaid if one of them goes into a nursing home.
Before the high court's decision, spousal financial protection rules were unavailable to same-sex couples if their state of residence did not recognize their marriage. With a semi-private room in a nursing home costing $80,000 a year, many couples can easily wipe out all their assets without such protection. Continue reading
Conference offers occasion, sources to report on key issues of aging
The Older Americans Act – signed into law on July 14, 1965 – mandated a national conference on aging every 10 years. I've attended the past two White House Conferences on Aging (1995, 2005), and this decade's event is far different from the previous ones.
This conference was preceded by five, one-day, invitation-only "forums;" prior conferences featured hundreds of federally sanctioned local events. At the one-day forums, mornings were spent listening to national and local experts, then attendees separated into special interest groups for the afternoon to discuss – and then report back on – one of four designated topics. Here is how one attendee assessed the forum in Boston. Continue reading
Can screenings be too much of a good thing?
Medicare reimburses for cancer and other screenings as part of routine care for older adults. Of course, the goal is to catch diseases in their earliest, most treatable stages. But can preventive care become too much of a good thing?
For years, evidence has grown about wasted Medicare dollars on needless screenings. The Center for Public Integrity found that 40 percent of Medicare spending on common cancer screenings are unnecessary – costing billions of taxpayer dollars. Continue reading
Experts stress increased need for more long-term care initiatives, funding
In a run-up to the July 13 White House Conference on Aging, (WHCOA) policy experts are stressing the need for increased funding for long-term services and supports (LTSS).
G. Lawrence Atkins, Ph.D. executive director, Long-Term Quality Alliance and president, National Academy of Social Insurance, reviewed key lessons from the Federal Commission on Long-Term Care and aging services innovations to frame future care delivery.
Atkins is the former chair of the commission, which issued a comprehensive report in 2013 calling for more funding and services for care and caregivers of older adults. About 78 percent of adults over age 65 has some type of unmet care need, requiring help with independent household activities of daily living. More than a quarter of older adults rely on outside help; 75 percent turn to family members to help meet their needs. Continue reading
Report emphasizes worldwide toll of dementia on women
A new report highlights just how much more likely women are to be affected by dementia than men around the world. Not only are the majority of people living with or at risk of developing the disease female, but women are also the majority of caregivers and health professionals in most countries.
"Women and Dementia: A Global Research Review" from Alzheimer's Disease International (ADI) calls for a broader, evidence-based approached to female-targeted dementia health programs. The need is particularly strong in low- and middle-income countries, where female-led caregiving is the principal care model. Continue reading
Seniors struggle to access adequate dental care
Many Americans lose their private dental benefits when they retire.
But Medicare, the nation's health insurance program for seniors, does not cover routine dental procedures.
The situation leaves millions of elders, living on fixed incomes, making hard choices about when to seek care – and, as in Thelma Chappell's case, postponing a dental visit until the pain gets too bad to ignore. Continue reading


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JOURNAL OF CLINICAL MICROBIOLOGY, Oct. 2010, p. 3517–3524
Copyright © 2010, American Society for Microbiology. All Rights Reserved.
Vol. 48, No. 10
Highly Sensitive and Quantitative Detection of the H274Y Oseltamivir
Resistance Mutation in Seasonal A/H1N1 Influenza Virus?
Darwin J. Operario,1Michael J. Moser,2† and Kirsten St. George1*
Laboratory of Viral Diseases, Wadsworth Center, New York State Department of Health, Albany, New York,1and
EraGen BioSciences, Madison, Wisconsin2
Received 21 May 2010/Returned for modification 6 July 2010/Accepted 21 July 2010
A C-to-T transition mutation in the neuraminidase gene from seasonal A/H1N1 causes a His-to-Tyr mutation
at amino acid position 275 (H274Y, universal N2 numbering), conferring resistance against oseltamivir
(Tamiflu). This mutation was first detected in clinical samples in Europe during the 2007-2008 influenza
season. Viruses with this mutation reached a prevalence of ?11% by the end of the season in North American
isolates tested by the CDC. We developed a highly sensitive and specific quantitative real-time reverse
transcriptase PCR assay to detect the H274Y mutation. This assay utilizes a 5?-methyl-isocytosine (isoC)
residue and fluorescent reporters on genotype-specific primers. During PCR, a quencher coupled to isoguanine
(isoG) is site-specifically incorporated complementary to the isoC/dye, resulting in loss of fluorescence.
Optimization of primers and assay conditions produced a limit of detection of 100 gene copies per reaction for
both wild-type and H274Y genotypes. In samples with mixed populations, it can reliably detect as little as a 1%
wild-type or 0.1% H274Y component. This high sensitivity makes the assay usable on samples with viral loads
too low for dideoxy or pyrosequencing analysis. Additionally, the assay distinguishes seasonal A/H1N1 from
A/H3N2, influenza B, or 2009 pandemic A/H1N1, making it useful for influenza virus subtyping as well as for
drug resistance detection. We probed seasonal A/H1N1 samples from the 2005-2006, 2006-2007, and 2007-2008
influenza seasons. Data from the new assay closely matched available drug resistance genotype data previously
determined by dideoxy sequencing. The H274Y mutation was only found in samples from the 2007-2008 season.
Influenza viruses cause considerable annual worldwide mor-
bidity and mortality. In the United States alone, greater than
200,000 persons are hospitalized each year due to influenza
and approximately 36,000 die from influenza-related disease
(16). Vaccination is considered the first and best defense
against influenza. However, the efficacy of protection con-
ferred by annual vaccination can be limited by the strength of
the antigenic match of vaccine strains to the circulating strains.
In addition, herd (community-level) immunity is limited by less
than 100% vaccine coverage. These circumstances allow influ-
enza to easily spread among susceptible persons and through
populations. Antiviral drug treatment and prophylaxis are ad-
ditional and necessary modes of defense against morbidity,
mortality, and further spread of the virus.
Widespread resistance against the adamantane class of drugs
among A/H3N2 viruses, beginning in the 2003-2004 season,
prompted public health officials in the United States to recom-
mend against the use of these drugs during the 2005-2006
season in favor of neuraminidase inhibitors (NAIs) (3). First
approved for clinical use in the United States in 1999 (17),
NAIs target the viral surface protein neuraminidase and are
effective against both influenza A and B. There are currently
two FDA-approved drugs in this class: oseltamivir (Tamiflu;
Roche) and zanamivir (Relenza; GlaxoSmithKline). When
given within the first 48 h of a patient becoming symptomatic,
NAIs have been shown to reduce the duration and severity of
influenza illness in both adults and children (11, 25).
In January 2008, nine European countries reported seasonal
influenza A/H1N1 isolates showing resistance to oseltamivir
(13, 25). In the following months, additional countries, includ-
ing the United States, reported oseltamivir-resistant influenza
viruses (24). These findings were alarming because drug resis-
tance testing during the previous influenza season (2006-2007)
had revealed no oseltamivir resistance in Europe (13). Fewer
than 1% of North American seasonal A/H1N1 viruses from the
same time period tested by the Centers for Disease Control
and Prevention (CDC) showed resistance (4). In addition, dur-
ing clinical trials with oseltamivir, shedding of drug-resistant
virus was noted at a frequency of only 4% in children and in
?1% in adults (28). Analysis revealed that all resistant viruses
were seasonal A/H1N1, carrying the same C3T transition
mutation in the neuraminidase gene, with a resulting histidine-
to-tyrosine change at amino acid position 275 ("H274Y" in
universal N2 numbering). By the end of the 2007-2008 season,
the CDC reported this mutation in 111 of 1,020 tested seasonal
A/H1N1 isolates; 4 were found in New York State (4). Osel-
tamivir-resistant seasonal A/H1N1 spread extensively, becom-
ing the dominant variant in Oceania and Southeast Asia in
May 2008 (10) and with virtually all seasonal A/H1N1 strains
possessing the H274Y mutation during the 2008-2009 influ-
enza season in the United States (5).
The need for continual monitoring for antiviral drug resis-
tance among influenza viruses is highlighted by several factors.
Use of antiviral medications as a treatment and prophylactic is
an integral component of infection control during influenza
outbreaks. With the advent of adamantane resistance, use of
* Corresponding author. Mailing address: David Axelrod Institute,
Wadsworth Center, New York State Department of Health, P.O. Box
22002, Albany, NY 12201-2002. Phone: (518) 402-2709. Fax: (518)
473-4336. E-mail: firstname.lastname@example.com.
† Present address: Lucigen Corporation, 2120 W. Greenview Dr.,
Suite 9, Middleton, WI 53562.
?Published ahead of print on 28 July 2010.
Page 2
the neuraminidase inhibitors, most commonly oseltamivir,
has risen dramatically. In addition, oseltamivir is a major
component of influenza pandemic preparedness stockpiles
in the United States. Continual improvement of methods for
the survey and detection of oseltamivir resistance in all influ-
enza isolates is imperative to ensure the lasting viability of this
drug as an antiviral treatment.
Laboratory testing for NAI resistance can be performed via
a phenotypic neuraminidase inhibition assay, giving a 50%
inhibitory concentration (IC50) for a drug of interest. Labora-
tories can also use nucleic acid testing such as real-time quan-
titative reverse transcriptase (RT)-PCR as well as dideoxy se-
quencing and pyrosequencing methods. Sequencing methods
provide detailed information about gene regions of interest
and enable the identification of new mutations that may have
an effect on drug resistance. Pyrosequencing, in particular, has
been reported to be capable of detecting minor populations at
concentrations as low as 10% within mixtures of neuramini-
dase gene targets (7, 8). However, both dideoxy sequencing
and pyrosequencing require additional processing steps after
an initial PCR, as well as specialized sequencing instruments,
software, and training. Real-time quantitative RT-PCR pro-
vides a rapid, highly sensitive, and specific alternative to se-
quencing. This method is also capable of detecting targets in
samples with viral loads too low for detection in sequencing
assays. Furthermore, real-time platforms are available from
multiple manufacturers and are more commonplace in clinical
laboratories than sequencing equipment.
Here, we describe the development of a novel, highly
sensitive real-time quantitative RT-PCR assay for the de-
tection of H274Y in seasonal A/H1N1 influenza. The assay
utilizes MultiCode technology, a system that employs an addi-
tional base pair set of 5?-methyl-isocytosine (isoC) and isogua-
nine (isoG) (12, 22). An isoC and isoG can only base pair to
one another, and neither an isoC-G nor a C-isoG bond can
form. In real-time RT-PCR applications, an isoC base is in-
cluded at the 5? end of a primer, directly linked to a fluoro-
phore, such as 6-carboxyfluorescein (FAM) or hexachlorofluo-
rescein (HEX) (Fig. 1). During PCR, a quencher (Dabcyl)
directly linked to an isoG nucleotide is site specifically incor-
porated into the PCR product complementary to the isoC-
fluorophore, resulting in a detectable decrease in fluorescence
(23). Because multiple fluorophores can be used in the same
reaction, multiplexed assays can be designed. In addition, be-
cause the fluorophores are not cleaved from the oligonucleo-
tides, postamplification melt curve analysis is possible, facili-
tating analyses of assay specificity. This PCR technology has
been successfully employed as part of a PCR panel targeting
several respiratory viruses (15), in assays for other viruses
including hepatitis C (20) and HIV (19, 26, 27), and in the
detection of anthrax-related toxin genes (18).
This new assay was developed with analyses for both muta-
tion detection and facilitation of resistance evolution in mind.
To that end, we developed a novel multiplexed assay with high
sensitivity and specificity and with a higher sensitivity for de-
tecting a minor component mutant population compared to
that detectable by pyrosequencing. The assay was applied to a
collection of A/H1N1 influenza-positive samples collected be-
tween 2005 to 2008, in order to determine whether the H274Y
mutation was present in clinical samples prior to its apparent
emergence during the 2007-2008 season.
Specimens and standard curve construction. A total of 85 primary specimens
and isolates used in this study were collected in New York State during the
2005-2006, 2006-2007, or 2007-2008 influenza season as part of normal surveil-
lance. Only samples that were previously characterized as seasonal A/H1N1
influenza virus by the Wadsworth Center Virus Reference and Surveillance
Laboratory using real-time reverse transcriptase PCR were included in the
present study. For a subset of these samples, the neuraminidase gene had already
been characterized by dideoxy sequencing (14).
Cultured influenza isolates, used in assay development and validation (see
below), were maintained on rhesus monkey kidney cells (ViroMed Laboratories,
Minnetonka, MN, or Diagnostic Hybrids, Athens, OH). Neuraminidase inhibi-
tor-resistant influenza was maintained with oseltamivir carboxylate at a final
concentration of 10 ?g/ml (25.9 ?M; kindly provided by Roche). Virus cultures
were maintained until cytopathic effect was evident throughout the culture and
then harvested.
Nucleic acids were extracted using the QIAcube (Qiagen USA, Valencia, CA)
automated extractor with the Qiagen QIAamp viral RNA extraction kit accord-
ing to manufacturer's instructions. Influenza A/H1N1 isolates to be used for
controls and standard curve construction were first quantified in a separate
quantitative real-time RT-PCR assay targeting the matrix gene segment. Stan-
FIG. 1. Primer alignment to target sequence. Primers were designed against a consensus sequence of N1 sequences from seasonal A/H1N1-
positive clinical samples submitted for testing to the Wadsworth Center between 2004 and 2008. The genotype-specific primers are designed in two
sections: one section anneals to the coding section of the N1 gene, and there is a noncoding tail which includes an isoC residue and a fluorophore.
The WT-targeted primer is labeled in HEX, while the mutant-targeted primer is labeled in FAM. The nucleotide at the 3? end of each of the
genotype-specific primers targets the SNP responsible for coding for His or Tyr. A specific primer is used for reverse transcription. The same primer
is paired against the genotype-specific primers during PCR. This primer system creates an amplicon of 66 bp.
Page 3
dard curves were constructed by testing 10-fold serial dilutions of target, con-
taining between 108and 1 gene copy per reaction. Standard curve reactions were
applied to the multiplexed assay in duplicate, and each test was performed at
least three times.
Primer design. Primers were designed against a database of compiled seasonal
N1 gene sequences derived from New York State samples spanning from 2004 to
2008 and with the HYTHER online nucleic acid hybridization program, acces-
sible at http://ozone3.chem.wayne.edu. The resulting primer designs used in the
final assay are shown in Fig. 1. A specific primer was used for reverse transcrip-
tion. The same primer was also used during PCR and was paired with genotype-
specific primers. The primer targeted against wild-type (WT) N1 was labeled
with HEX, while the H274Y-targeted primer was labeled with FAM. PCRs with
these primers give a 66-bp amplicon.
Real-time reverse transcriptase PCR. The real-time quantitative reverse trans-
criptase PCR was performed with the Plexor one-step RT-PCR system from
Promega (Promega, Madison, WI). The reverse transcription step was per-
formed in a 20-?l volume including Plexor system components, 5 ?l target, and
primer (Fig. 1) to a final concentration of 0.4 ?M, at 45°C for 15 min, followed
by 95°C for 2.5 min. After reverse transcription, FAM (0.4 ?M final concentra-
tion)- and HEX (0.15 ?M final concentration)-labeled primers were added,
giving a final PCR volume of 25 ?l. All PCRs were performed on the Bio-Rad
iQ5 real-time PCR detection system (Bio-Rad, Hercules, CA). The cycling con-
ditions are as follows: 2.5 min at 95°C, 2 cycles of 5 s at 95°C and 35 s at 53°C,
and 45 cycles of 5 s at 95°C and 35 s at 63°C. PCR was followed by melt curve
analysis ranging from 55°C to 95°C, with 30 s per step with a 1°C increase per
step. The iQ5 real-time PCR software is designed to interpret and display data
to the user from reactions that show increases in fluorescence over time. Because
the PCR system used in this study is based upon decreases in fluorescence over
time, data from real-time PCR runs were imported into Promega Plexor soft-
ware, version 1.1.4, for data analysis. When using MultiCode PCR chemistry,
fluorescence is reduced with target amplification and is reported in relative
fluorescence units (RFU). In the MultiCode PCR developed in this study for
resistance mutation analysis, to define a sample as positive for the target, two
criteria must be met: (i) the fluorescence trace on the amplification plot must
cross the (user-defined) threshold line (giving the sample a defined threshold
cycle [CT] value), and (ii) the corresponding trace on the melt curve graph must
show a peak at 73 ? 1°C. As with all real-time PCR assays, CTvalues are
inversely proportional to the target concentration.
Specificity. Specificity was tested by application of the assay to a panel of
nonseasonal A/H1N1 viruses, including the following: influenza B; influenza
A/H3N2; 2009 pandemic A/H1N1; adenoviruses 3, 4, 5, and 8; coxsackieviruses
A9, B3, B4, and B5; human metapneumovirus 1B and 2B; rhinovirus 1A; measles
virus; mumps virus; severe acute respiratory syndrome (SARS) coronavirus;
coronavirus 229E; lymphocytic choriomeningitis virus; enteroviruses 68 and 71;
and echoviruses 6, 9, 11, and 30. Each virus in the panel was tested at a
concentration of at least 106genome copies per reaction, in duplicate. Specificity
experiments were performed at least three times, except for coxsackieviruses A9
and B5, coronavirus 229E, human metapneumovirus 2B, and lymphocytic cho-
riomeningitis virus, which were tested only twice. Seasonal A/H1N1 virus con-
taining WT or H274Y neuraminidase 1 genes was used as the positive control.
We utilized MultiCode technology to detect and quantify
seasonal A/H1N1 clinical samples containing populations of
WT or H274Y mutant neuraminidase genes. Primers were
designed against both the WT and mutant genotypes, such that
the nucleotide at the 3? end of each primer targets the single
nucleotide polymorphism (SNP) coding for either His or Tyr.
The primers are designed in two sections: an annealing seg-
ment that binds the coding region of the gene and a noncoding
tail that includes the isoC nucleotide and fluorophore. As
shown in Fig. 1, the neuraminidase-specific annealing region of
each primer has an annealing temperature of approximately
55°C. The 6-base noncoding tails increase the overall annealing
temperature by approximately 10°C, giving the primers a final
melting temperature (Tm) of approximately 65°C. The WT-
and H274Y-specific primers possess different tail sequences
and fluorophores. This overall primer design allows a two-
stage PCR cycling scheme (see Materials and Methods), which
begins with a lower-specificity cycling Tmof 55°C, followed by
additional cycles with higher specificity annealing/extension at
The assay employs a specific reverse transcription primer,
targeted against the negative-stranded viral RNA. The same
primer is also used during PCR, paired against both the WT-
and H274Y-specific primers. These primers yield a 66-bp am-
plicon on a seasonal N1 target. Melt curve analysis of ampli-
cons generated with either the WT- or H274Y-targeted primer
gives a product with a Tmof 73°C, as shown in Fig. 2.
Limit of detection. Optimization of primer concentrations
and cycling conditions under multiplexed conditions resulted
in a lower limit of detection of 100 WT or H274Y gene copies
per reaction. The limit of detection was determined using
standard curves constructed from quantified influenza virus
extracts from cultured isolates containing pure populations of
either WT neuraminidase or neuraminidase with the H274Y
mutation, as shown in Fig. 2. Some cross-reactivity of the
H274Y primer with WT template was observed at concentra-
tions of ?105WT gene copies. However, such cross-reactivity
is distinguishable due to the presence of a secondary melt peak
at 67°C, as shown in Fig. 2. When such cross-reactivity occurs
on samples with a pure WT neuraminidase genotype, the melt
peak at 67°C is stronger than the peak at 73°C.
Detection of mixed populations. Due to multiplexing, the as-
say is capable of detecting mixed populations within the same
reaction. To test assay sensitivity for mixed populations, quan-
tified WT and H274Y viral nucleic acid extracts were combined
at 1%, 5%, 50%, 95%, and 99% WT mixtures, with a total 106
gene copies per reaction. Additionally, 0.1% and 99.9% WT
mixtures were created, with 105, 106, and 107total gene copies.
The duplexed assay is capable of detecting a WT component as
low as 1% (104gene copies) in an otherwise mutant back-
ground (9.9 ? 105gene copies), as shown in Fig. 3A. As
demonstrated in Fig. 3C, the assay is also capable of detecting
a mutant population as low as 0.1% in an otherwise WT back-
ground containing 107, 106, or 105total gene copies.
Assay specificity. To test assay specificity for seasonal A/H1N1,
the assay was applied to a panel of nucleic acid extracts from
influenza and noninfluenza viruses. Figure 4 shows negative
signal results from a representative experiment. As can be seen
in this figure, there is a limited amount of nonspecific ampli-
fication for nonseasonal A/H1N1 samples, including those with
adenoviruses 4 and 8, coxsackievirus B3, enterovirus 68, and
A/H3N2 influenza virus. However, these nonspecific amplifi-
cations are easily distinguished from specific PCR products
generated on an N1 template, by melt curve analysis. The
primers used in this assay do not strongly cross-react with the
2009 pandemic A/H1N1 target, which can be attributed to
mismatches between the primers and the 2009 pandemic virus
N1 target. Analysis of the primer sequences reveals 11 mis-
matches between the reverse transcription primer and the 2009
pandemic virus N1 sequence and 2 mismatches for each of the
genotype-specific primers.
Analysis of archived influenza samples. The H274Y muta-
tion was first detected during the 2007-2008 influenza season,
but we were interested in investigating whether the mutation
had gone undetected in seasonal A/H1N1 samples collected
prior to the 2007-2008 season. A total of 85 archived respira-
VOL. 48, 2010H274Y MUTATION DETECTION USING isoC/isoG TECHNOLOGY3519
Page 4
FIG. 2. Limit of detection. The lower limit of detection was determined by application of the assay to standard curves from quantified nucleic
acid extracts to give between 108and 1 gene copy per reaction. Standard curve samples were derived from cultures containing seasonal A/H1N1
cultures with either pure WT or pure H274Y neuraminidase genes. The limit of detection for both the WT- and H274Y-targeted primers is 100
gene copies (gc), using the reaction conditions stated in Materials and Methods. In the amplification plots, data from a sample positive for the N1
target is shown by a loss of fluorescence (i.e., decreasing relative fluorescence units [RFU]) over increasing PCR cycles. In melt curve analysis, a
sample positive for an N1 target gives a melt peak at 73°C. Cross-reactivity of H274Y primer against a WT target gives a secondary melt peak at
67°C. (A) WT primer; (B) H274Y primer. Blue, amplification on a WT target; green, amplification on a mutant target; red, no-template control.
Page 5
tory samples positive for seasonal A/H1N1 were identified for
testing with the new assay. These samples had been submitted
to the Wadsworth Center for testing during the 2005-2006,
2006-2007, and 2007-2008 influenza seasons. Available dideoxy
sequencing data on the N1 gene sequences from 2006-2007 (17
specimens) and 2007-2008 (44 specimens) seasonal A/H1N1-
positive samples (14) were used as a basis for comparison to
the data generated from the MultiCode-based assay.
Results from the new assay indicated that the 44 specimens
from the 2007-2008 season contained either a 100% WT or
100% H274Y neuraminidase genotype. As shown in Table 1,
the data generated by the new assay on samples from the
2007-2008 season closely matched available sequencing data.
Importantly, the four samples that had previously tested pos-
itive for the H274Y oseltamivir resistance mutation by dideoxy
sequencing also tested positive for the mutation by the new
assay. The new assay was additionally able to identify as WT
one sample that dideoxy sequencing had failed to characterize
at all. A total of 41 additional seasonal A/H1N1-positive re-
spiratory specimens from the 2005-2006 (4 specimens) and
2006-2007 (37 specimens) seasons were retrieved from sample
archives at the Wadsworth Center. The new assay detected
only WT neuraminidase in all samples from these seasons. No
samples of mixed genotype were found in samples from any of
the three seasons.
The adamantanes have been approved as antiinfluenza drugs
since 1966 (1) and were commonly prescribed prior to recom-
mendations against their use during the 2005-2006 season.
Amino acid changes in the M2 protein provide resistance to
these drugs and have been reported to emerge within as little
as 6 days after the initiation of rimantadine prophylaxis (2, 9,
21). Resistance to the NAI oseltamivir was first detected in the
2007-2008 influenza season among seasonal A/H1N1 viruses,
all with the H274Y neuraminidase mutation. Viruses possess-
ing the H274Y mutation spread rapidly, even in the absence of
drug pressure, and oseltamivir-resistant seasonal A/H1N1 be-
came the dominant virus of this subtype during the 2008-2009
season. In addition, the H274Y mutation in seasonal A/H1N1
is the equivalent of the H275Y mutation detected in some 2009
pandemic A/H1N1 viruses.
Common molecular methodologies for identifying influenza
virus and detecting drug-resistant strains include dideoxy se-
quencing, pyrosequencing, and quantitative real-time PCR.
Used as part of quantitative reverse transcriptase PCR, the
FIG. 3. Mixed-sample analysis. For panels A and B, nucleic acid
extracts from pure populations of WT and H274Y viruses were mixed
in ratios of 1%, 5%, 50%, 95%, and 100% WT with 106gene copies in
total and applied to the multiplexed assay. Black, 1% WT; orange, 5%
WT; brown, 50% WT; green, 95% WT; blue, 99% WT; red, no-
template control. In panel C, nucleic acid extracts from pure popula-
tions of WT and H274Y viruses were mixed in a ratio of 99.9% major
component to 0.1% minor component genotype with 107, 106, or 105
total gene copies (gc) applied to the multiplexed assay. Purple, 107
gc total; pink, 106gc total; orange, 105gc total; red, no-template
control. Data from these plots are interpreted as described in the
legend to Fig. 2.
VOL. 48, 2010 H274Y MUTATION DETECTION USING isoC/isoG TECHNOLOGY3521


text:
Serie A
Serie B
Champs Lge
Live Scores
Fantasy Football
Napoli change defence at Chievo
Napoli make the trip to Chievo without Paolo Cannavaro and Miguel Angel Britos, so must revamp their defence.
Petkovic: 'Lazio want third place'
Vladimir Petkovic has one foot in the Europa League quarter-finals and warns tomorrow's opponents Fiorentina that "Lazio will try to take third place with all our might."
Serie B: Sassuolo seven-goal thriller!
Sassuolo won a seven-goal thriller at promotion-chasing Varese, while Modena had a 3-3 draw, Verona won away and Padova were stunned.
Strama: 'Inter back on track'
Andrea Stramaccioni is confident Inter can put the Tottenham humiliation behind them against Bologna and insists Antonio Cassano is not overweight.
Montella: 'I admire Petkovic'
Vincenzo Montella prepares Fiorentina for tomorrow night's Euro showdown with Lazio and admits "Vladimir Petkovic is someone I admire very much."
Maran: 'Catania can upset Juve'
Rolando Maran insists Catania "still believe in Europe" and can get a result against Juventus tomorrow afternoon.
Moratti: 'Strama responded well'
Inter President Massimo Moratti confirmed his faith in Andrea Stramaccioni. "The Coach responded positively to the pressures of the role."
Anelka back to face Catania
Nicolas Anelka and Paolo De Ceglie are back in the Juventus squad against Catania, but Arturo Vidal misses out.
Kroldrup: 'My career was at risk'
Danish international Per Kroldrup begins again at Pescara and admits his injury "risked ending my career early."
Milner: 'How Man City handled Balotelli'
Milan and Italy pay attention, for James Milner has revealed the secret to keeping Mario Balotelli out of trouble. "It was like having a 12-year-old in the dressing room."
Mazzarri: 'Milan comeback normal'
Walter Mazzarri feels it is "only normal a superpower like Milan is getting closer," as Napoli take on Chievo to keep hold of second place.
Balotelli: 'What Mancini meant...'
Mario Balotelli asked referees for more protection, praised Giampaolo Pazzini and replied to Manchester City manager Roberto Mancini.
Raiola: 'Pogba staying at Juve'
Paul Pogba has been linked with Manchester City among others, but agent Mino Raiola assures "he has found the right environment at Juventus."
Pazzini micro fracture?
Giampaolo Pazzini scored a goal despite limping last night, but there are fears the Milan striker may have suffered a micro fracture.
Moratti wants Leonardo back
Inter celebrate their 105th birthday today and it's reported they are trying to tempt Leonardo back from PSG as director general.
Conte: 'I'm committed to Juve'
Antonio Conte believes the Scudetto race is still very open, that tomorrow they'll face "Real Catania" and assures he is staying with Juventus despite foreign interest.
Arsenal join Perin chase
Arsenal are the latest club to be linked with an offer for Genoa-owned goalkeeper Mattia Perin, who is on loan at Pescara.
Corini: 'Napoli stronger than Juve'
Eugenio Corini believes Scudetto-chasing Napoli "are potentially stronger than Juventus" as they visit Chievo on Sunday.
Rossi: 'It's just not football'
Cagliari-Sampdoria will be played behind closed doors on Sunday, but Blucerchiati Coach Delio Rossi complains "it's just not football."
Guidolin: 'Super Udinese' to beat Roma
Francesco Guidolin warned it will take "a super Udinese" to end Roma's run of three wins on the bounce in this evening's Serie A game.
Ballardini rages at ref
Davide Ballardini blasted the referee after Genoa's defeat to Milan and claimed "we should've had three penalties."
Allegri: 'If Napoli slip up...'
After victory in Genoa, Max Allegri admitted Milan are targeting second place, told off Kevin Constant for his dismissal and began the build-up to Barcelona.
Serie A Previews: Di Natale v Totti
Roma look for a fourth consecutive victory, but are Antonio Di Natale's favourite victims, while Juventus-Catania and Lazio-Fiorentina shake up the Euro race.
Today's headlines:
What the papers say...
Today's front page headlines from La Gazzetta dello Sport, the Corriere dello Sport and Tuttosport.
Zapata: We can catch Napoli
Cristian Zapata has claimed that Milan are aiming for second place in Serie A after their victory over Genoa.
Montolivo: It was a tough match
Milan midfielder Riccardo Montolivo has praised the goals of Giampaolo Pazzini and Mario Balotelli as his side defeated Genoa.
Paloschi thinking only of Chievo
Chievo striker Alberto Paloschi has insisted that he is only concentrating on the club's survival.
Cavani has second child
Napoli striker Edinson Cavani has become a father for the second time this evening, after the birth of his newborn son.
10-man Milan beat Genoa
Goals from Giampaolo Pazzini and Mario Balotelli were enough to give 10-man Milan a 2-0 win over Genoa.
Lichtsteiner wants to avoid Bayern
Juventus defender Stephan Lichtsteiner has admitted that he would like to avoid Bayern Munich in the next round of the Champions League.
Inter celebrate 105th birthday
Inter President Massimo Moratti has praised the club's fans as they prepare to celebrate their 105th birthday.
Genoa fined €100,000
Genoa have been fined €100,000 after their match against Siena last season was briefly suspended when fans entered the field of play.
'Only Juve have something more'
Stevan Jovetic has claimed that 'only Juventus' have a better squad than Fiorentina, as the Viola look to close in on a Champions League place.
Line-ups: Genoa - Milan
Milan have rested Kevin Prince-Boateng and Antonio Nocerino for tonight's game against Genoa, ahead of their Champions League clash with Barcelona.
Pjanic: Roma can finish third
Roma midfielder Miralem Pjanic believes that the Giallorossi have what it takes to qualify for the Champions League.
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Serie A Week 7: Did You know?
Dominant Euro-Juve show capability
Sassuolo set the example
How the mighty have fallen
Sizing up the Contenders - Part Two
Serie A Week 6: Did You Know?
Sizing up the Contenders - Part One
Are Inter just getting lucky?
Serie A Week 5: Did You Know?
Frosinone shock the world
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Besides Babies R Us, where is everyone looking for a comfy rocker to sit and nurse in? I don't want to spend more than $200. Where else can we look for cute nursery (inexpensive) items?
Rocker for the nusery? Other cute decorations?
Walmart has one that is like $150 that is one of those glider chairs with the glider footstool. I love those! They are so comfy! I think Target has one that is comperable for around the same price.
Amanda(28)� Emery(37) married: 03/17/10
2 Angel Babies: 12/15/08 @ 6 weeks; 6/20/09 @ 15 weeks, DSD Kaylee (8); DSD Cyanna (5)
Elijah Jacob Due 05/14/11
Burlington Coat Factory has a nice one in the baby depot section that is a glider as well. I think it was 100 for the rocker and 40 or 50 for the foot stool.
Casey (25) DH Jason (29)
Jade (9) Addison (2) Spencer (6/29/13)
I own the one from walmart and it worked great with my DD. It was less than $200 and we still use it at night to read books before bedtime.
SAHM to S 8/05/09 O 5/19/11
FYI, On Black Friday last year Babies R Us had a Shermag glider/ottoman set for half price. It was regular $300 and we got it for $150. We love it! See if you can find a 2010 black friday ad for babies r us online. Maybe they'll do it again!
mommy to Reece Samuel 1/1/10 and...
Lila Cate3/30/11 (6.5 weeks early)
A man and woman had a little baby, yes they did, they had three in the family, and that's a magic number...
We have one that we bought through target online when odd was born 7.5 years ago. It has been used almost everyday since and is in perfect condition for this baby. My neighbor has the same one (she has a son the same age as odd) and they love it, too.
ETA: It was around $150
We have a large wooden rocker in DS's room we got as a gift from my parents when he was born. Also while I was pregnant with him my parents were getting new furniture in their living room so we got their "old" glider/foot stool set. I loved it and used it all the time to nurse in.
As for other accessories...we bought DS's crib at Target. I found his wooden toy box at a craft fair. I wanted a different type of dresser for him instead of the pressed wood ones you find now so I went to an antique store, found a cool looking one with glass knobs and talked the price down to $120. DH refinished it and it looks great! I plan to look for dressers again at flea markets or antique stores for this LO as well. Such neat designs can be found...
For decoration in DS's room, I found wooden letters to spell out his name at Hobby Lobby and painted them colors to match his bedding. They can adhere to the walls just using sticky puddy - no nails needed!
Try looking for a rocker in places that aren't necessarily for baby. Anything labeled "baby" are automatically way more expensive. Look in furniture stores, antique stores, flea markets, yard sales, craig's list, overstock.com etc.
My phone does that weird caps thing on every word. No idea why. Sorry.
we got our rocker from the Amish store in town. It's WONDERFUL... super strong and sturdy. ours was a little over $300, but they had some less expensive


text:
Cedar Park is an affordable alternative to living in Austin and offers much nicer views, a lot less traffic, and a down-home country feel that you just don't get living near 6th Street. With new developments like the 1890 Ranch shopping center and the proximity to Cedar Park Regional Medical Center, you will find Cedar Park Town Center to be a perfect mix of location and amenities.
Surround yourself with nature trails and lakes instead of sidewalks and skyscrapers. Most of the neighborhood's homes were built in the last few years, so there's a newer, modern feel to the area. Cedar Park Town Center homes are considered perfect for first-time homebuyers and couples expanding their families because of the award-winning school district and amenities like the built-in community playgrounds.Cedar Park Town Center is the perfect combination of family living and natural landscapes for the outdoor enthusiast to enjoy golf, tennis, kayaking, rock climbing, and everything in between. Come on, y'all–Texas living doesn't get much better than this.
Cedar Park Town Center Market Statistics
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Note : Statistics represent residential listings only. Search all Cedar Park Town Center Real Estate Listings here.


text:
Health Care: At his press conference this week, President Obama tried to reassure Americans about ObamaCare. Instead, he displayed either an incredible lack of understanding about his own law, or something far worse.
Asked about increasing Democratic concern over what Sen. Max Baucus called a looming ObamaCare "train wreck," Obama claimed that it's all much ado about very little.
But almost nothing he said in defense of ObamaCare was accurate. Among his statements:
"A huge chunk of it's already been implemented."
In fact, all that's been implemented so far are a few PR-friendly changes like a mandate to cover children up to age 26 and a more generous Medicare drug benefit.
Democrats put off the bulk of the law — the massive market regulations, the government-run exchanges, mandates to buy coverage, and various taxes and fees—until 2014, both to hide its true costs and to avoid any unpleasantness before the 2012 elections.
"For the 85% to 90% of Americans who already have health insurance ... they don't have to worry about anything else."
Really? The Congressional Budget Office expects 7 million workers — and possibly as many as 20 million — will lose their employer coverage because of ObamaCare. That's plenty to worry about.
The Centers for Medicare and Medicaid Services said millions of seniors will get dumped from their private Medicare Advantage plans by 2017 thanks to sharp payment cuts required by the law.
And small businesses now providing coverage face huge rate hikes thanks to ObamaCare's many market regulations and benefit mandates. Maryland's biggest insurer, nonprofit CareFirst BlueCross BlueShield, said ObamaCare will force rates up by 15% next year.
"The other stuff's been implemented and it's working fine."
That's only true if you ignore the fact that ObamaCare's high-risk pools have been a disaster, attracting a third as many people as predicted while costing far more than the administration budgeted.
Or the fact that Obama had to issue more than 1,200 waivers to companies who said the law's initial insurance market rules would have forced them to cancel coverage for millions of workers.
The overly complicated small-business tax credit has also been a bust, with only about 5% of eligible firms taking advantage of it. And so on.
"We're going to be able to drive down costs ... and that will save the country money as a whole over the long term."
Except, Obama's own health care number crunchers say ObamaCare will force national health spending up 7.4% in 2014, and add billions in costs over the next decade. The Congressional Budget Office says it will add massively to federal health spending.
And the architects of Obama's reform wrote in the New England Journal of Medicine about how, despite ObamaCare, "health costs remain a major challenge."
It all leads one to wonder: Is Obama just dangerously misinformed about ObamaCare? Or is he willfully misleading the country?
Equality: California's new equal-pay law has everyone buzzing, especially Hollywood. But this law and others like it don't address one of the biggest equal-pay issues: the growing gap between government and private workers.Actress Gwyneth Paltrow has expressed dismay that she doesn't earn as much ...
Revolt: New polls show that over half of GOP voters favor the political outsiders running for president. They like candidates with real-world experience, and Carly Fiorina, Ben Carson and Donald Trump have that in abundance.The trio of non-politicians continues to lead the GOP pack. But for the ...
Scandal: We have now learned that Hillary Clinton's private email server was subject to multiple cyberattacks from abroad. Does anybody really believe that hackers didn't gain access to classified material?As first reported by the Associated Press, a cybersecurity firm hired to protect Clinton's ...
Congress: Republicans' go-along-to-get-along establishment lost when Rep. Kevin McCarthy unexpectedly withdrew from the race for Speaker of the House. Now is the time for strong, principled leaders to step forward.McCarthy was such a shoe-in for speaker, seemingly from the moment John Boehner ...
You win the presidency, Richard Nixon supposedly observed, by tacking to the right in the primaries and to the center in the general election. Hillary Clinton seems to be following that strategy except, as a Democrat, she is tacking to the left. This strategy has risks, as Nixon, who lost the ...
Investor's Business Daily provides exclusive stock lists, investing data, stock market research, education and the latest financial and business news to help investors make more money in the stock market. All of IBD's products and features are based on the CAN SLIM® Investing System developed by IBD's Founder William J. O'Neil, who identified the seven common characteristics that winning stocks display before making huge price gains. Each letter of CAN SLIM represents one of those traits.


text:
This Commercial bakery equipment is in good condition, worked great when last tested prior to restaurant closing. Please note: we have no way of currently testing the item; however, we do offer a 30 day warranty. Item retails for $30,000 new.
Call/ Text 404.662.1289
Oct 7 -
Atlanta :
Unbelievable deals and steals at our upcoming auction of used restaurant equipment and used grocery store equipment. We are the nation's top seller of used equipment like this, and we have a live auction twice a month (2nd and 4th Tuesdays) at our Georgia warehouse.
Coming up (10/13/15) Tuesday evening at 6pm EST, we have over 350 lots for sale including BRAND NEW popcorn machines, hot dog ...
Oct 5 -
Atlanta :
We are selling our Coffee House/ Bistro/ Internet Café, with limitless possibilities with your personal touch. This convenient location in the heart of Carrollton, GA has an awning covered entrance from our outdoor patio with seating area. We also have an 1,800 square foot indoor café with seating for 35 and lounging couches and chairs for studying or checking emails etc. Our Bistro is in a ...
Aug 29 -
Atlanta :
Type K Pancake Dispenser
The Professional's Choice for accurate portioning
The restaurant industry's favorite for depositing Pancake, Waffle and Waffle Cone batter - now available for only $167
Product Description
PDF) http://www.belshaw-adamatic.com/catalog/retail-equipment/pancake-dispenser---type-k/type-k-pancake-dispenser-the-professionals-choice-for-accurate-portioning
Aug 28 -
Clarksville :
Used Hobart Stainless Steel Cooler w/Rack -
VisEq

This is part of a used grocery store equipment, restaurant equipment, and convenience store equipment sale happening on August 25 at 6pm. Come join us at:
Vision Warehouse
4191 West Street NW
Covington, GA 30014
If you can't make it in person, and believe me you will not want to miss this spectacle, you can preview and bid now ...
Aug 24 -
Atlanta :
We'll be sending text message alerts for your subscription to


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On The Podiumhttps://www.smartsound.com/strata/images/STRATPWP_200.jpgSingle7100061-ST$ 39.957100061-EX$ 69.957100061-W$ 149.95Instrument: Bass (Electric), Brass Section, Drums, Guitar (Electric), Percussion, Strings, Synthesizer, Instrumental, Guitar Genre: Funky, Retro, Corporate/Technology, Vintage, Disco, Funk Keywords: Driving, Energetic, Happy, Positive, Cool, Cheerful, Competitive, Confident, Motivating, Pulsating, Fun, Festive Production Types: Commercials, Corporate, Corporate Communications, Product Videos, Training, Background
On The Podium from the album Power Play
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Yankee Doodle from the album American Spirit
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Ambitious Plan from the album Thrive
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Great Strides from the album Thrive
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Inner Power from the album Thrive
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BridgeStreet Panoramic Heights
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TripAdvisor Reviews BridgeStreet Panoramic Heights London
Travel Blogs from London
Tuesday Zooday
... One of those things is that the working population of London is 14 million people and I think they were all out in their cars because it took us an hour and a half to get as far as a 15 min walk is! In fact, because the play was tonight, we had to get off the bus instead of going to the palace because there's no way we would ever make it.
The play was in a really beautiful theater called ...
I left my heart in San Francisco (or maybe TOKYO)
... toss between Tokyo and Hoi an of Vietnam. Of the two independence days we experienced, Vietnam's had a better atmosphere, but shhhh don't telll America. One of the the biggest features of our trip being so enjoyable has been the people we've met, who've all been class craic (they should all know what that means now!) I'm not a fan of gushing, but this has been one of the best experiences of our lives. Thanks for reading Josh and ...
Hallowed Turfs
... we found Lord's, and a couple of minutes after that we arrived at the gate we could go in at. We joined the 10 o'clock tour, along with lots of Indians and a few Australians. It's always interesting to see behind the scenes, and the tour took us inside the hallowed halls, even if photography was a bit restricted. But stay off the grass! Afterwards we had lunch at Lord's Tavern next door, but we forgot to use the discount voucher we got ...
A little London on less than a little sleep
... and I could not have asked for more on such a quick visit to London. I have to thank friends for suggesting the walk, it was a great way to catch a sense of some of London in such a short time.
Walking London for the first time I came to appreciate the 'Look Left' , ' Look Right'. Painted on the road in front of every crosswalk. The streets are so busy and it's really easy to get disoriented as to which way ...
M&Ms and a girl called Cath
... in Newcastle, the Lake District and York and then slowly make my way back to London.
Turns out that 4 months of camping wears you out. So I decided to set that aside for a wee while and just stop. I need a break.
So I am back at my home away from home.
And I am stuffed.
Other places to stay in London
Marlin Apartments - Empire SquareSouth Bank/Bankside, London | 4 star condofrom $225
Grand Plaza Serviced ApartmentsBayswater, London | 4 star condofrom $215
- Good location, less than great experience
- Nice hotel, bad, very bad staff
- Loved the place
Chelsea CloistersChelsea, London | 3 star condofrom $162
Marlin Apartments Queen StreetFleet Street/Old Bailey, London | 4 star condofrom $281
MiNC - Mayfair Court Serviced ApartmentsMayfair/Soho/West End, London | Condofrom $312
Marlin Apartments Canary WharfLondon East, London | 4 star condofrom $203
Think Tower BridgeGrange, London | 4 star condofrom $202
SO QuartierNorthwest/Regent Park, London | 4 star condofrom $161
The King`s Wardrobe Serviced Apartments by BridgeStreetFleet Street/Old Bailey, London | Condofrom $275
Market View by BridgeStreetIslington/Angel/King's Cross, London | 4 star condofrom $297
Grand Residences by Marriott - Mayfair - LondonMayfair/Soho/West End, London | 5 star condofrom $694
Somerset Roland GardensKnightsbridge/Earl's Court/Kensington, London | 4 star condofrom $78
Marlin Apartments Stratford LondonLondon East, London | 4 star condofrom $170
Marlin Apartments LimehouseStepney, London | 4 star condofrom $167
146 SuitesNorthwest/Regent Park, London | 3 star condofrom $163
Access Apartments CityLondon City, London | 3 star condofrom $134
MiNC Cock LaneFarringdon Without, London | 4 star condofrom $335
Groveland House ApartmentsFleet Street/Old Bailey, London | Condofrom $574
Hyde Park Suites Serviced ApartmentsBayswater, London | Condofrom $139
Think SE1 ApartmentsSouth Bank/Bankside, London | 4 star condofrom $222
Point West Apartments by Crown LawnKnightsbridge/Earl's Court/Kensington, London | 4 star condofrom $244
Presidential ApartmentsFairfield, London | 3 star condofrom $295