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Accelerated methylation of ribosomal RNA genes
during the cellular senescence of Werner syndrome
Laboratory of Molecular Genetics, National Institute on Aging, National Institutes of Health,
Baltimore, Maryland 21224, USA
has been implicated in cellular and organismal aging.
The role of rDNA in premature and normal human
aging was investigated by measuring rDNA gene copy
number, the level of rDNA methylation, and rRNA
expression during the in vitro senescence of primary
fibroblasts from normal (young and old) donors and
from Werner syndrome (WS) patients. In compari-
son to their normal counterparts, WS fibroblasts
grew slowly and reached senescence after fewer
doublings. The rDNA copy number did not change
significantly throughout the life span of both normal
and WS fibroblasts. However, in senescent WS and
normal old fibroblasts, we detected rDNA species
with unusually slow electrophoretic mobility. Cellu-
lar aging in Saccharomyces cerevisiae is accompanied
by the formation and accumulation of rDNA circles.
Our analysis revealed that the rDNA species ob-
served in this study were longer, linear rDNA mole-
cules attributable to the inhibition of EcoRI cleavage
by methylation. Furthermore, isoschizomeric restric-
tion analysis confirmed that in vitro senescence of
fibroblasts is accompanied by significant increases in
cytosine methylation within rDNA genes. This in-
creased methylation is maximal during the abbrevi-
ated life span of WS fibroblasts. Despite increased
methylation of rDNA in senescent cells, the steady-
state levels of 28S rRNA remained constant over the
life span of both normal and WS fibroblasts.—
Machwe, A., Orren, D. K., Bohr, V. A. Accelerated
methylation of ribosomal RNA genes during the
cellular senescence of Werner syndrome fibroblasts.
Ribosomal DNA (rDNA) metabolism
Key Words: life span ? aging ? population doubling ? rDNA
The role of ribosomal DNA (rDNA) in human
aging has received considerable attention in recent
years because of the central role played by its gene
products in protein synthesis. Eukaryotic cells con-
tain 100–300 copies of rDNA genes per haploid
genome. These genes show a high degree of se-
quence homology and are arranged in gene clusters,
along with other components for ribosome assembly
in the nucleolus.
It has been speculated that aging is accompanied
by changes at the rDNA locus. The proximity of
multiple rDNA genes to one another makes them
potentially susceptible to homologous recombina-
tion resulting in possible gain or loss of rDNA gene
copies. The rDNA copy number has been the focus
of a number of studies, many of which indicate that
there is a selective loss of rDNA during the course of
aging. An age-related loss of rDNA has been re-
ported in postmitotic brain tissues of beagle dogs
(1), human myocardium and cerebral cortex (2, 3),
and mouse brain, spleen, and kidney tissues (4).
Recent studies have implicated changes in rDNA in
the cellular senescence of Saccharomyces cerevisiae
(reviewed in ref 5). Extrachromosomal rDNA circles
(ERCs) accumulate in budding yeast during cellular
senescence and the introduction of exogenous ERCs
accelerates yeast aging (6). Studies of sgs1 deletion
mutants in yeast show increased recombination at
the rDNA locus (7, 8), an accelerated accumulation
of ERCs (6), and a significant reduction in the
average life span (9). Thus, changes in the rDNA
copy number have been associated with cellular
senescence in yeast.
The in vitro senescence of primary fibroblasts has
been used as a model system for mammalian aging
studies. A correlation exists between the life span of
a species and the number of times its cells can divide
in culture (10, 11). Also, several studies report that
the replicative capacity of cells in culture generally
decreases with increasing age of the donor (10, 11).
However, this view has been challenged by recent
studies (12) that find no correlation between donor
age and in vitro life span. Nonetheless, a connection
between cellular senescence and organismal aging is
supported by the premature aging phenotype of
patients with Werner syndrome (WS). Patients with
1Correspondence: Laboratory of Molecular Genetics, Na-
tional Institutes on Aging, NIH, 5600 Nathan Shock Dr.,
Baltimore, MD 21224, USA. E-mail: firstname.lastname@example.com
Page 2
WS have an early onset of characteristics of aging
such as skin atrophy, early graying and loss of hair,
and increased incidence of a number of age-related
diseases such as malignant neoplasms, atherosclero-
sis, type II diabetes mellitus, osteoporosis, and cata-
racts (13–15). Primary fibroblasts established from
WS patients divide fewer times in culture than cells
derived from normal individuals (16, 17). Notably,
the gene that is defective in WS (known as WRN) is
a human homologue of the aforementioned yeast
SGS1 gene. Both WRN and Sgs1 are members of a
family of RecQ-like DNA helicases (7, 8, 18) and have
DNA helicase activity in vitro (19, 20). Although the
exact function of WRN in humans is unknown, the
hyper-recombination of rDNA genes in sgs1 mutants
suggests that the lack of a functional WRN protein in
WS cells may result in hyper-recombination at the
rDNA loci. That mutations in the yeast SGS1 and
human WRN genes can cause premature aging phe-
notypes suggests that there may be a common con-
served mechanism of aging involving changes in
rDNA in both yeast and mammals.
Other changes at the rDNA loci may be relevant to
organismal and cellular aging. The level of expres-
sion of rRNA genes could influence ribosomal func-
tion and protein synthesis. Base modifications (such
as methylation) within rDNA sequences may affect
the expression of rRNA genes. The extent of meth-
ylation in promoters of RNA polymerase II (pol II)
-transcribed genes has been shown to influence
transcription (21–23). Some studies have shown in-
creases in methylation in promoters of specific pol
II-transcribed genes during in vivo and in vitro aging
(24, 25), despite the finding that overall genome
methylation levels decrease both with age in animal
tissues and during cellular senescence (26, 27).
Although an increase in rDNA methylation in aging
mouse tissues has been reported (28), the methyl-
ation of rDNA genes during in vivo or in vitro aging
has not been examined in detail. It is also unclear
whether methylation of rRNA genes influences RNA
polymerase I (pol I) transcription.
Our main aim was to study the role of rDNA in
premature and normal human aging by looking for
changes in rDNA genes during cellular senescence.
We have examined the rDNA gene copy number,
expression level, and methylation during the in vitro
senescence of primary fibroblasts derived from both
normal individuals (young and old donors) and WS
patients. We find that both the copy number of
rDNA genes and the steady-state levels of 28S rRNA
remain constant throughout the in vitro life span of
both normal and WS fibroblasts. However, senescent
normal and WS fibroblasts show significant increases
in the methylation of cytosine in rDNA genes. Nota-
bly, this increase in methylation is most pronounced
in the abbreviated life span of WS fibroblasts.
Cell types and culture conditions
Normal human fibroblast cultures GM08429 (1-day old do-
nor) and GM08447 (2-day old donor) were obtained from the
National Institute of General Medical Sciences Cell Reposi-
tory. The WS primary fibroblast cultures (AG03141 and
AG00780) and additional normal human fibroblast cultures
AG11744 (84-year old donor) and AG05247 (87-year old
donor) were obtained from the National Institute on Aging
Cell Culture Repository (Coriell Institute). For each set of
fibroblasts, the phenotype, donor age, and sites of biopsy are
listed in Table 1. All cultures were grown in minimal essential
medium with Earle's salts (Gibco BRL, Grand Island, N.Y.)
supplemented with essential amino acids (0.25 ?g/ml), non-
essential amino acids (0.25 ?g/ml), vitamins (1 mg/ml)
(Gibco BRL), 15% fetal bovine serum (Gibco BRL), penicil-
lin (100 U/ml), and streptomycin (100 ?g/ml) (Gibco BRL).
Cultures were maintained at 37°C in a humidified atmo-
sphere containing 5% CO2and had viabilities of greater than
90%, as judged by the trypan dye exclusion assay.
Isolation of genomic DNA
Viable cells (1–5?106cells) were washed twice with phos-
phate-buffered saline (PBS), suspended in lysis solution (0.5
M Tris [pH 8.0], 1% sodium dodecyl sulfate, 0.5 mg/ml
proteinase K) and incubated at 37°C for 16–24 h. Next,
saturated NaCl (1⁄4 volume) was mixed vigorously with the
lysate, and the samples were centrifuged at 2500 rpm for 30
min. DNA in the supernatant was precipitated with ethanol
and the precipitate was collected by centrifugation, washed
with 70% ethanol, and resuspended in 1 ml of TE (10 mM
Tris-HCl [pH 8.0],1 mM EDTA). The DNA was subsequently
treated with 100 ?g of RNase A for 3 h at 37°C, reprecipitated
(with 0.2 volumes of 11 M ammonium acetate and 2–2.5
volumes of ethanol), and washed with 70% ethanol. The DNA
was finally dissolved in 100–200 ?l TE and concentration
determined by UV spectrophotometry. The relative purity of
each DNA sample was ensured by examining the 260/280 nm
absorption ratio. For determining the rDNA copy number, 2
?g of DNA was incubated with EcoRI (10 units) in the
appropriate restriction buffer for 16–20 h.
Analysis of methylation by 5-methylcytosine-sensitive
restriction enzymes
The restriction endonucleases HpaII and MspI were used to
study sequence-specific methylation patterns by Southern
blot analysis as described previously (28, 29). HpaII will cut
the sequence CCGG but is inhibited if the internal cytosine is
methylated, whereas MspI cuts both CCGG and CmeCGG
efficiently (30). In parallel, equivalent amounts (1 ?g) of
TABLE 1. Fibroblast cultures used in this study
cultures PhenotypeDonor age Biopsy site
1 day
2 day
84 year
87 year
29 year
59 year
Skin (right ear)
Skin (right ear)
Skin (upper arm)
Skin (upper arm)
Skin (unknown)
Skin (unknown)
1716Vol. 14September 2000MACHWE ET AL.The FASEB Journal
Page 3
genomic DNA were incubated with either HpaII or MspI in
buffers specified by the manufacturer for 16–20 h at concen-
trations of 4–5 units/?g of DNA. Southern blotting tech-
niques (described below) were used to compare HpaII-di-
gested DNA side-by-side with MspI-digested DNA for changes
in methylation of CpG sites in CCGG sequences.
Southern blotting and hybridizations
After restriction endonuclease digestion, DNA samples were
electrophoresed on agarose gels (0.5, 0.8, or 1%). After
electrophoresis, the gels were soaked in 0.25 M HCl for 30
min, then in denaturing solution (1.5 M NaCl, 0.5 N NaOH)
for 45 min, and finally in neutralizing solution (1 M Tris [pH
7.4], 1.5 M NaCl) for 45 min. The DNA was transferred to
nylon membranes (Hybond N; Amersham, Little Chalfon,
U.K.) using a posiblot apparatus (Stratagene, San Diego,
Calif.) with 10 ? SSPE (1.5 M NaCl, 0.1 M NaH2PO4, 0.01 M
EDTA) as the transfer buffer (31). After UV cross-linking of
DNA to the membrane, the blots were incubated in Hybrisol
I (Oncor) for 12 h at 45°C, followed by hybridization for
12–14 h in the same solution supplemented with ? 107dpm
denatured probe/ml. After hybridization, the membranes
were washed 1) twice with 2 ? SSPE, 0.1% sodium dodecyl
sulfate (SDS) for 15 min at 25°C, 2) once with 0.1 ? SSPE,
0.1% SDS for 15 min at 25°C, and 3) twice with 0.1 ? SSPE,
0.1% SDS for 30 min at 60°C. The membranes were rinsed in
2 ? SSPE and air dried. Radioactivity associated with specific
28S rDNA and dihydrofolate reductase (DHFR) bands was
measured using a PhosphorImager (Molecular Dynamics,
Sunnyvale, Calif.) and quantitated with ImageQuant software.
Preparation and analysis of RNA
Metabolically active cells (1–3?106) were washed twice in
PBS, scraped from the petri dish in 1 ml STAT-60 (Teltest-B),
and subsequently frozen at ?70°C. After thawing, 0.3 ml
chloroform was added, the sample was mixed vigorously for
15 s and the organic and aqueous phases were separated by
centrifugation at 14,000 g for 20 min at 4°C. The aqueous
phase was collected and RNA was precipitated in 0.7 ml
isopropanol at ?20°C overnight. The precipitate was col-
lected by centrifugation at 14,000 g for 10 min at 4°C, rinsed
briefly in 75% ice-cold ethanol, air dried, and finally resus-
pended in 30–100 ?l of sterile DEPC-treated water. The
concentration of the RNA samples was measured by UV
spectrophotometry. RNA (1 ?g) was electrophoresed on
denaturing gel systems using formaldehyde and formamide as
described previously (32). The gels were scanned using a
fluoroimager (Molecular Dynamics) to measure ethidium
bromide fluorescence and the amount of rRNA (28S) in each
lane was determined.
DNA probes
A 1.8 kb DHFR genomic DNA probe was isolated from
plasmid pBH31R1.8 (obtained from Dr. Giuseppe Attardi,
California Institute of Technology) as previously described
(33). The 6.7 kb rDNA probe from rat recognizing a 7.1 kb
EcoRI fragment in the human sequence (Fig. 1A) was from
the plasmid pEE6.7, described elsewhere (34). Probes were
labeled to a high specific activity (?1.9?109dpm/?g) with
[?-32P]-dCTP using a random prime labeling kit (Rediprime
II, Amersham Pharmacia Biotech) as specified by the manu-
Growth characteristics of normal and WS
To determine whether in vitro senescence is accompa-
nied by changes at the rDNA locus, we examined
primary fibroblasts derived from both normal (young
and old) donors and WS patients (Table 1). The WS
Figure 1. A) Human rDNA gene map (one repeat ? 42.9 kb),
determined from GenBank accession number U13369. The
locations of the 28S, 18S, and 5.8S rDNA genes are indicated
by stippled boxes and the positions of the EcoRI restriction
sites (E) are marked. The distances (in kb) between EcoRI
sites in the rDNA repeat unit are noted above. The location of
the rDNA probe used in Southern blotting is depicted below.
B) Southern blot analysis of total genomic DNA isolated from
selected PDLs of primary fibroblast cultures of GM8429
(1-day-old donor), GM8447 (2-day-old donor), AG05247 (87-
year old donor), and AG03141 (WS patient). Genomic DNA
(2 ?g) was digested with EcoRI and separated by electrophore-
sis in 0.8% agarose gels. DNA was transferred to membranes
that were hybridized with [32P]-labeled rat rDNA probe.
Radioactivity associated with DNA species containing rDNA
was visualized by phosphor imaging. The numbers above each
lane refer to the individual PDLs analyzed. DNA size markers
(HindIII-digested ? DNA) are indicated to the right of the
bottom right panel. C) A direct comparison of the Southern
blots of PDL#27 of AG03141 (WS), and PDL#52 of AG05247
(87-year-old donor), highlighting the slower migrating rDNA
species only observed in samples from senescent cultures of
old and WS fibroblasts.
1717 rDNA CHANGES DURING CELLULAR AGING
Page 4
designations of AG00780 and AG03141 have been
confirmed by genetic analysis (35) and Western blot-
ting (36), respectively. Cells from each individual fibro-
blast culture were counted at near confluence and
subcultured twofold, with each successive split consti-
tuting one population doubling (PDL). This proce-
dure was repeated until the growth of each culture was
negligible. As the growth rate of each culture slowed,
increasing numbers of cells exhibited morphological
changes typical of senescent cells (37) including en-
larged size and greater numbers of cytoplasmic micro-
filaments. In 'senescent' cultures, incorporation of
bromodeoxyuridine (BrdU) into DNA was undetect-
able (by immunofluorescence using an anti-BrdU an-
tibody) in the vast majority of cells, confirming their
lack of replicative potential (data not shown). Our cell
culture protocol allowed the collection, examination,
and comparison of cells and biological (DNA and
RNA) samples from normal and WS fibroblasts at
successive points during the entire replicative life span
of each individual culture. At selected passages, total
genomic DNA and RNA were isolated from normal
and WS cultures, and purified DNA samples were
analyzed for changes in copy number and methylation
state at the rDNA locus. RNA samples from early and
senescent, normal and WS fibroblasts were compared
to determine if there were changes in the steady-state
levels of rRNA.
Primary fibroblasts established from WS patients
grew very slowly and senesced after relatively fewer
PDLs than fibroblasts from normal individuals. Both
WS fibroblast cultures had an extremely slow growth
rate after ? 15 PDLs and negligible growth at 26–27
PDLs. This is in agreement with the previously
reported premature replicative senescence observed
in cells from WS patients (16, 17). The growth
characteristics of normal fibroblast cultures in our
study showed no direct correlation between donor
age and in vitro life span. This is exemplified by the
relatively short replicative life span of a GM8447
(2-day-old) and the long life span of an AG05247
(87-year-old) donor (Table 1). These findings sup-
port a recent study by Cristofalo et al. (12).
The rDNA copy number does not change
significantly with PDLs
primary fibroblasts from normal and WS donors is
accompanied by changes in the rDNA copy number.
We selected four primary fibroblast cultures from nor-
mal individuals (Table 1). GM8429 and GM8447 were
from young (1- and 2-day-old, respectively) donors and
AG11744 and AG05247 were derived from old (84- and
87-year-old, respectively) donors. Two primary fibro-
blast cultures, AG03141 and AG00780, from patients
with WS were also examined (Table 1). Genomic DNA
was isolated from normal and WS primary fibroblasts at
selected PDLs and restricted with EcoRI. Southern blot
analysis was carried out using equal amounts of DNA
from serial passages of each set of fibroblasts. A probe
for rDNA sequences including the 5.8S rDNA gene, an
internal transcribed spacer region, and most of the 28S
rDNA gene (Fig. 1A) detected a 7.1 kb EcoRI fragment
in each DNA sample. Representative Southern blots
from four sets of fibroblasts are shown in Fig. 1B. The
same blots were cohybridized with a probe to the single
copy housekeeping gene, DHFR. The hybridization
intensity of the DHFR fragment in the same lane was
used to correct for differences in loading of DNA
samples. The corrected rDNA hybridization intensities
at selected PDLs of duplicate sets of each fibroblast
culture are presented in Table 2. Due to differences in
hybridization conditions, specific activities of probes,
and exposure times, the numerical values for rDNA
hybridization intensity between individual experiments
on different blots (i.e., Expt. 1 and Expt. 2 for each
fibroblast culture) are not directly comparable. The
mean rDNA hybridization signal for each set of fibro-
blast cultures was determined (Table 2) and the vari-
ance from this mean (normalized to unity) at succes-
sive PDLs for each replicate series of fibroblasts is
plotted in Fig. 2. Despite some experimental variation,
this data clearly shows no systematic increase or de-
crease in the rDNA copy number during the in vitro life
span of these fibroblast cultures. Notably, both WS
fibroblast cultures maintained the same relative rDNA
copy number throughout their in vitro life span. A
similar analysis of rDNA copy number in fibroblasts
from GM8447 (2-day-old donor) and AG11744 (84-
year-old donor) also showed no significant change in
rDNA copy number during cellular senescence (data
not shown). Hence, there are no significant changes in
rDNA copy number over the course of in vitro senes-
cence of any normal (young and old donors) or WS
fibroblasts examined in this study.
We observed some slower migrating bands that
hybridized with our rDNA probe in a few samples
(Fig. 1C). These additional bands could easily be
discerned in genomic DNA isolated from the most
senescent passages of cultures from old individuals
(84- and 87-year old donors) and WS patients. In
these cultures these rDNA species could also be
detected (albeit much less prominently) in the DNA
purified from the passages leading up to senescence.
It is important to note that we could not detect any
change in the restriction pattern of the DHFR gene
regardless of passage (data not shown). This suggests
that regions outside of the rDNA locus are efficiently
cleaved by EcoRI even in genomic DNA from the
senescent passages, thus diminishing the possibility
of nonspecific inhibition of restriction occurring in
the DNA samples isolated from senescent cultures.
These slower migrating species could either be
1718 Vol. 14September 2000MACHWE ET AL.The FASEB Journal
Page 5
longer, linear rDNA fragments or nonlinear rDNA
structures, perhaps similar to ERCs in yeast or
blocked replication/recombination intermediates.
To determine the nature of these slow migrating
species, DNA samples from the senescent cultures of
normal old donor (AG05247) and WS patients
(AG03141 and AG00780) were electrophoresed
through 0.5% and 0.8% agarose gels, along with
Figure 2. Variance of relative rDNA
hybridization signal during the in
vitro life span of primary fibroblasts.
From serial passages of four individ-
ual sets of fibroblasts, genomic DNA
was analyzed on duplicate Southern
blots as described in Fig. 1B. The
intensity of rDNA-hybridizing bands
for each sample was normalized
based on the intensity of the single
copy DHFR-hybridizing fragment in
the same lane. For each replicate
series of fibroblasts, the mean rDNA
hybridization signal was determined
(see Table 2). The variance from the
mean (normalized to unity) for each
series over the entire course of in
vitro life span of GM8429 (1-day-old
donor), AG05247 (87-year-old do-
nor), AG03141 (WS), and AG00780
(WS) cultures is graphically repre-
sented in the individual panels.
TABLE 2. Constancy of rDNA throughout the lifespan of primary fibroblasts
GM8429 (normal young)
PDL# 121517 192123 272931 3335 3738Mean
AG05247 (normal old)
PDL#2528 3034 38 41 4345 47 4951 52Mean
PDL#13 17 19 212325 2627 Mean
PDL# 13 16 1820 2224 26 Mean
aValues correspond to relative rDNA hybridization signals after correction with single copy DHFR gene. Individual experiments cannot be
directly compared due to differences in hybridization conditions including specific activity of probes and varying exposure times. ND, not
determined; se, standard error.
1719rDNA CHANGES DURING CELLULAR AGING


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Who is genealogy for? For me? Sure, I love doing it for myself and for others. To discover the past, those persons who made me, those persons who contributed to my makeup ... it is a fantastic journey of "OMG I'm related to that person!?!".
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1 week ago


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VIDEO: Ride-in Dalek range invades Earth
Doctor Who is big business these days, for young and old, and we've seen all manner of tied-in merchandise at Pocket-lint, from Lego-style minifigs to a Wiimote in the shape of The Doctor's trademark Sonic Screwdriver. However, none have been quite so bonkers, yet cunningly brilliant as Kids@Play's Ride-in Dalek.
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Khushi and Arnav
Khushi and Arnav in Iss Pyaar Ko Kya Naam Doon? TV Show
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TV Show Iss Pyaar Ko Kya Naam Doon?
Celebrity : Sanaya Irani , Barun Sobti
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Love u Barun
Proud to b a Sobtian.
!!!!! Miss you Sanaya Irani lots and lots !!!!!!
Sanaya Irani
Waiting for for you to back on TV !!!!
Edited on:23 May 2015 at 7:30pm
Love Arshi...& Miss them
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Missing you so much.!!!!!!
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Love you loads Barun !!!!
Stay Blessed !!
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Come back to our screens soon
Arnav/Barun had such a lovely, warm aura around him!


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D Squad places 1st at Gus Macker 3-on-3 tournament in Norwich, NY on July 12th.
The 18 year old team of Michael Warchol, Jerome Brabham, Tyvon Reed and Jalen Hawkins
placed 1st place with a record of 5 wins and 1 loss.
The 12 year old team of Josh Martin, Tanner Russin, Louis Robinson and Roy Robinson
placed 1st place with an undefeated record.
The 9th grade team finished 2 - 3 and the 11/12th grade team finished 3rd in the 2015 Continental Cup.
Anthony Giglio places 2nd in the 3 point shooting contest at the 2015 Continental Cup.
Anthony was the 2014 Continental Cup champion. Congrats to Anthony and his performance in the 3 point shooting contest.
The 2015 Continental Cup slam dunk competition was dominated by D Squad with Zach Sawyer taking 1st place and Terrence Walker taking 2nd place. Congrats to Zach and Terrence for their outstanding performance at the slam dunk competition.
10th grade team places 2nd in the Continental Cup lead by Darvin Lovett Jr and Mekhi Warren.
The team entered the the Cleveland Cavs Quicken Arena on Sunday, July 5th undefeated and the number one seed. They fought a hard game and lost by 2 points to VAC of Cleveland, Ohio. Congrats to Coach Peterson and the team.
D Squad has strong showing at 2015 Continental Cup in Cleveland, Ohio.
2015 Practice Schedule:
D Squad Youth Basketball Organization


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Mother's Day Brunch at Los Angeles Hotels
April 27, 2013
Updated on May 6, 2015
Los Angeles hotels are offering some of the best brunches in town for mom's special day. To go with the lavish buffets and brunches, many hotels are featuring special kids' menus and activities for the whole family. All of the following Mother's Day brunches will be offered on Sunday, May 10, 2015 unless otherwise noted.
Cafe Sierra at Hilton Los Angeles/Universal City
Celebrate Mother's Day in style with an unparalleled brunch experience! An extensive buffet at the Hilton's Cafe Sierra includes seafood, assorted cheeses, fruit, carving stations, Chinese specialties, pasta, sushi/sashimi station, cappuccinos, orange juice and champagne. Mom even receives a long-stemmed rose. Brunch is served 10:30 a.m. - 3 p.m., $56 adults, $28 children ages 3-10. Dinner 5-9 p.m., $56 adults, $28 children ages 3-10. Reservations: 818.509.2030.
Millennium Biltmore Hotel
Celebrate mom with a special afternoon tea held inside Bernard's at the Millennium Biltmore Hotel. Tea service includes a variety of sandwiches, French macarons, madeleines, cream puffs, tarts and scones and choice of premium teas. 2-5 p.m. $39 per person. Enjoy a festive brunch inside Smeraldi's Restaurant. Amid a backdrop of the Italian Renaissance decor, mom will enjoy a sumptuous champagne brunch buffet and long-stemmed rose treat. 10:30 a.m. - 2 p.m. $59 adults, $25 children ages 4-11. Reservations: 213.612.1562.
Esterel Restaurant at Sofitel Los Angeles
The deluxe Mother's Day brunch at Esterel Restaurant located inside the Sofitel Hotel is an impressive event. From the delicious Amuse-Bouche to French style dessert offerings, mom will love the elegant dining experience at one of L.A.'s premier destinations. Live jazz music will be performed by the Miles Davis Jazz Band. Reservations recommended. $75 per person, includes bottomless Mimosas.
Luxe Sunset Boulevard Hotel
Enjoy al fresco dining at On Sunset in the Luxe Sunset Boulevard Hotel this Mother's Day, featuring live jazz music. A buffet menu includes an omelet station, carving station, antipasto display, Viennese table, dishes like Atlantic Sea Bass and Moroccan Grilled Chicken, vegetables and more, along with a complimentary glass of cava, mimosa or peach bellini. Complimentary 2-hour parking available. 10 a.m. - 2:30 p.m. $66 adults, $30 children under 12. Reservations: 310.691.7584.
Crowne Plaza Redondo Beach & Marina Hotel
At the Crowne Plaza Redondo Beach, you, mom and the whole family will love Splash Bistro's made-to-order omelets and waffles; pasta station; delectable appetizers and salads; a build-your-own gravlax station; and chef's carving specialties. Don't forget their decadent desserts, which you'll wash down with orange juice, coffee or tea, and a glass of Champagne. 11 a.m. to 2:30 p.m. $40 adults, $20 children (ages 5-12, under 5 free). Free 3-hour self-parking with hotel validation. Reservations: 310.318.7764.
Four Seasons Hotel Los Angeles at Beverly Hills
Treat your mother to a day in Beverly Hills with an elaborate brunch at Culina, Modern Italian at the Four Seasons Hotel Los Angeles at Beverly Hills. Enjoy a buffet of offerings mom is sure to love – think farmer's market salads, fresh seafood and artisan California cheeses. Mom can indulge in dishes like Belgian waffles, crab benedict with Choron sauce and slow roasted leg and shoulder of lamb – this is her day, after all! Finally, Pastry Chef Federico Fernandez's elaborate dessert station, featuring everything from house made doughnuts and gelato to French pastries and a chocolate fondue station, is certain to sweeten her day. The young ones will enjoy their own buffet, which includes angus beef and cheese sliders, organic buttermilk chicken tenders and more. 10 a.m. to 3 p.m. $98 adults, $49 children ages 5-12. For an extra $25, guests can also get bottomless mimosas. Reservations: 310.860.4000.
The Portofino Hotel & Marina
Mother's Day brunch inside the Portofino Hotel Bayside Ballroom is an extravagant buffet, with items including free-flowing sparkling wine mimosas, jumbo shrimp prawns on ice, a prime rib and honey smoked ham carving station, decadent desserts, a kid's buffet and of course, those ocean views. 9:30 a.m. - 3:30 p.m. $62 adults, $22 children (ages 4-12, under 4 complimentary). For those who prefer to order from a menu on Mother's Day, BALEENKitchen is serving an a la carte brunch menu that includes cornmeal pancakes, huevos redondos, and pain perdu. Don't miss the popular All-You-Can-Drink Bloody Mary Bar with 20 hot sauces from around the world! Bottomless mimosas also available. Live music begins at 11 a.m. Reservations: 310.372.1202.
The View at Manhattan Beach Marriott & Golf Club
Celebrate mom at The View Restaurant, located at the Manhattan Beach Marriott & Golf Club, including continental breakfast favorites, omelet and crepe station, vegetables, fresh biscuits, seafood and salad buffet, selection of desserts and complimentary glass of sparkling wine for mom. Unlimited bellinis or mimosas are an additional $10. $45 adults, $15 children under 12.
The Belvedere at The Peninsula
Treat mom to a lavish day at The Peninsula Hotel, featuring a 3-course meal at The Belvedere that includes free-flowing champagne. Menu highlights include lobster rolls, sorrel chicken, veal blanquette and a dessert buffet. 11 a.m. - 9 p.m. $135 adults, $48 children under 12.
The Langham Huntington Pasadena, Los Angeles
Bountiful Mother's Day Brunch in the Huntington Ballroom at The Langham is a lavish spread of delicious eats from seafood to carving stations and much more. Free-flowing champagne and a chocolate fountain provide the finishing touch. 10:30 a.m. - 2:30 p.m. $98 per person, $32 for children ages 4-12. The Terrace Restaurant features its Dim Sum Brunch on Mother's Day, where guests can indulge in an omelet and carving station, dim sum, Peking Duck and more. Dim Sum Brunch: 10 a.m. to 2 p.m. $58 per person, $29 for children 4-12. Indulge in a Pink Chocolate Afternoon Tea inside The Lobby Lounge, where mom will savor delicious tea sandwiches and a scrumptious dessert bar. The highlight is the grand pink chocolate fountain, surrounded by chocolate sculptural art. 11 a.m. - 4 p.m. $59 adults, $29.50 children 4-12. An interactive Sunday Market Brunch in The Royce Wood-Fired Steakhouse includes a Mimosa station, Bloody Marys and several chefs stations. 11 a.m. to 3 p.m. $92 per person, $32 for children ages 4-12. Reservations: 626.585.6218.
Viceroy Santa Monica
Settle in for a relaxing and luxurious Mother's Day brunch at CAST Restaurant at the Viceroy Santa Monica. Highlights of the decadent buffet set-up include a carving station, omelet station, pancake, waffle and French toast station, seafood, salads and traditional breakfast dishes, as well as small touches like tea sandwiches and desserts. A kids' buffet will offer waffle sticks, macaroni and cheese and chicken fingers. 11 a.m. - 4 p.m. $65 adults, $25 children 10 and under (excluding tax and gratuity) Reservations strongly recommended: 310.260.7511.
Hyatt Regency Century Plaza Breeze Restaurant
Turn an afternoon into something extraordinary at Hyatt Regency Century Plaza's Breeze Restaurant. Enjoy a Champagne Brunch featuring a seafood station of sushi, oysters, crab legs, jumbo shrimp and smoked salmon; a carving station with pepper crusted prime rib and wild mushroom turkey roulade; an omelet bar, fresh fruit and cheese display and Chef Felix's assorted desserts. A knee high kids buffet rounds out the options and leaves everyone with smiles. Sparkling wine, coffee, tea, juice and soda are included. 10:00 a.m.-2:00 p.m. Buffet costs $75 per adult; $45 for seniors 65 and older; $25 per child ages four – twelve plus tax and gratuity. For children ages three and younger, the Mother's Day brunch is complimentary! Indoor and outdoor seating is available. Reservations: 310.551.3334.
Culver Hotel
Step inside the historic Culver Hotel's Crystal Dining Room for a mother's day brunch that's sure to impress! Treat mom to fantastic buffet that includes an omelete station, cowboy pancakes, wild salmon with lemon basil butter, peppercorn crust prime rib, a dessert station ad much more. Buffet costs $39 per person, $49 with bottomless mimosas and $19 for children under 12. 11:00 a.m.-Reservations: 310.558.9400.
Wolfgang Puck at Hotel Bel-Air
Experience pure luxury at Wolfgang Puck at Hotel Bel-Air on Mother's Day. Their three-course prix-fixe menu at $120 per person includes choices such as the Butter Poached Maine Lobster with sugar snap peas, asparagus, preserved Meyer lemon, sweet pea puree and sauce americaine, Hand Cut English Pea Tortelloni with mascarpone and parmigiano reggiano, and The H.B.A "Benedict" with poached organic eggs, white cheddar biscuit and maple hollandaise. 11:00 a.m.-3:00 p.m. Reservations: 310.909.1644.
Andaz West Hollywood
This Mother's Day, beginning at 7:00 a.m., Andaz West Hollywood will be inviting guests and neighbors in to indulge in a Bottomless Brunch. Toast to Mom on her special day with Bottomless Mimosas for only $15. The pros will do the cooking this year with a special fixed-price breakfast menu which will include fresh eggs, farmer's market whole fruit, Belgian waffles, pastries with house-made marmalade, coffee, and more. For more information, visit westhollywood.andaz.com
Pacific Palms Resort
Celebrate Mothers Day at Pacific Palms Resort located in City of Industry. Head to their Cima Restaurant for a scrumptious All You Can Eat Brunch on Sunday morning from 9:00 a.m.-4:00 p.m. Large family tables and patio dining will be available. Buffet costs $60 per person and $25 for children 12 and under. If you're looking for a place to treat mom to a lovely dinner, Pacific Palm's Red Restaurant has got you covered from 5:00 p.m.-9:00 p.m. $60 per person includes a Prix Fixe menu with delicious choices such as Maine Lobster Pasta, Crispy Skin King Salmon and Roasted Prime Rib. Complimentary champagne and a red rose for the guest of honor. Reservations: 626.854.2509.


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I know this is ridiculous, but I'm totally stressing on choosing a time for his party. This is the first party where he will have friends and I'm just not sure when is a good time to have the party. He has an almost 2-year old sister as well so I want to make sure everyone has a good time and it doesn't interupt naptime. Oh and it's on Sunday, so I have to avoid conflicts with people who attend church.
What do you think?


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Volvo Truck Plant Saves $1.2M in Energy Costs
The Department of Energy recognized Volvo Group North America for its leadership in energy efficiency at the New River Valley assembly plant near Roanoke, Va. Volvo Trucks' New River Valley assembly plant is the company's largest truck manufacturing plant in the world, covering more than 1.6 million square feet.
At the plant, energy efficiency has become part of the facility's core business practices, with an energy team member reviewing and approving every purchase made by the plant. Volvo also asked employees to suggest energy efficiency projects and has committed to reinvesting the savings from these projects – about $1.2 million to date – into future efficiency efforts.
In 2012, the New River Valley facility was one of the first facilities in the US to be certified under DOE's Superior Energy Performance program for facilities. New River Valley achieved a 26 percent improvement in energy efficiency in three years. As a partner in the Better Buildings Industrial Superior Energy Performance Accelerator, the Volvo Group is now implementing the Superior Energy Performance program at facilities nationwide.
As a Better Buildings, Better Plants partner, the Volvo Group has already achieved 16 percent savings across its US plants and continues working toward its goal of becoming 25 percent more energy efficient within ten years.
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No, not in Friedrichshain. NEAR Berlin. In a place called Großziethen. The Germans in this area call these phenomena "Kornkreise" and they appear to be designs or patterns laid down gently in a growing crop, leaving the surrounding crop untouched. 68 more words
Tags » Hustle (Hoax)
Call Of Duty Gamer Accidentally Takes Over German Missile Battery
Or he's just that good. Apparently the same guy who ruined the Bundestag's computer network last month, an overzealous German Call of Duty gamer has now managed to get a German Patriot missile battery stationed in Turkey to carry out several strange and "unexplained" orders. 58 more words
Greeks Apologize And To Pay Back All Debts Tomorrow
The nation of Greece said sorry to the European Union with a present of an enormous wooden horse.
Left outside the European Central Bank in the dead of night, the horse has now been moved into the ECB's central lobby where it is proudly on display. 90 more words
Queen Sighting In Berlin Sort Of
German Of The Day: Behindert
That's what Germans are. Or at least one out of eight Germans are these days: Disabled.
Not only are they getting more and more old and gray and in the way… 189 more words
15 Cool German Illnesses You Can Only Get Here
Mostly because 1) you probably can't pronounce them and 2) they don't really exist.
Germans aren't hypochondriacs, by the way. They're Hypochonder.
14. ZIVILISATIONSKRANKHEIT… 29 more words
German Family Father Films UFO That Turns His Kid's Face All Weird As It Flies By
"The fact that the person blurred out the face of the kid and didn't get the UFO in the dead center of the video is what causes me to believe this is real."


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Is this restaurant family-friendly?
Does this place serve seafood?
Is this restaurant good for dinner?
Is this restaurant good for lunch?
Does this restaurant serve shrimp?
Is this restaurant good for breakfast?
Is this restaurant good for brunch?
Does this restaurant offer takeout or food to go?
Does this restaurant offer free wifi?
Does this restaurant offer delivery?
Thanks for helping!
Small seafood restaurant chain in Southeast Georgia.
This is where we take visitors to Waycross. The seafood is always fresh and prepared good and the salad bar is huge! I love the blackened Grouper, fried Shrimp, onion rings, shrimp salad
My husband and I enjoy this restaurant when we get a chance for a night out and want seafood. Parking lot was full when we arrived, but we were seated right away. We were expecting a long wait for our food since there were a couple of parties in the back. We both ordered bite sized fried shrimp. Salad bar...
Growing up in a small town about 40 miles away, this was the go to place if you wanted to go eat seafood. Family oriented, wait staff always friendly, good value for the meals. Salad bar is always fresh, with a variety that some "chain" restaurant's are skimping on. Word of warning: don't get the buffet unless you are really...
They have a very friendly staff...our waitress was kristen and she did a wonderful job! The food was great...we had fried dinners but it is not heavy greasy breading so we did enjoy it ! The salad bar is wonderful with so many selections it is hard to try it all! We will be back !
My husband & I went here for lunch on Memorial day during an off hour (between 2 & 3PM). We ordered from the lunch menu (available until 4PM). I ordered the chicken quesadilla, seafood gumbo & onion rings. My husband ordered fantail shrimp, coleslaw, and onion rings. The onion rings were fresh battered, huge, crisp, and delicious. They were served...
I have been to Captain Joe's only twice before, because as a seafood lover I don't find it very good. This time I was surprised. I ordered the catfish and fantailed shrimp, both fried. the shrimp were great, but the catfish was just ok. The salad bar was good, but the baked potato was just average. I don't know when...
We had lunch here while in town for a class reunion. I had the seafood plate with shrimp, oysters and the best crab cake I've ever had. It also has a 40 item salad bar with just about anything you could ask for. The waitress was very friendly and attentive.
My wife and I ate shrimp and had it fried. Worse shrimp I've ever had. I'm from Fernandina Beach Fla and yes my family was shrimpers. So I know a little about shrimp. They were so bad I just asked for a box to go after the first bite. The service was, take order, bring disgusting food, check. The owner...
This restaurant has some of the best bite size shrimp we had had in quite a while. Everyone's food in our party was good. The lettuce on the salad bar could have been a little fresher. Other than that, I would rate this restaurant very high.
One thing I love about Waycross is that it has some of the best food you could possibly find. Captain Joe's has always been one of my favorite places to eat at back home. The soup and salad bar is the best you will ever come across. The only issue is that the restaurant has aged alot of the years...
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That's nothing - I've actually had the AI in combat BREAK without warning. Suddenly, my characters do the 'stay away from mob at all costs' dance, leaving me to be the only one fighting.
Of course, I'll only see this is I happen to glance at my radar, which I next to never do.
Fortunately, all it takes is for me to switch to them and give them a different AI plan. Normally, that kickstarts them again. But I've seen them go entire battles (and multiples of them) without the AI starting back up again.
And the AI is dumb. In Tales of Symphonia, they'd do a very good job of fighting on their own. Here... Oh boy...
That's also one of the few flaws I've seen in this game. Otherwise, it rocks.


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Post by Michelle of Avery and Augustine
Now that the whirlwind of getting ready for the start of another school year has come and gone, it's a good time to establish a regular routine for your kids' lunches with some basic back to school lunch planning.
I thought it would be great to get Avery involved in the planning, with hopes that it would get her excited about trying new foods and eventually get her ready to pack her own lunch (maybe in a couple of years—just planting seeds here!). Children really seem to take ownership and become more enthusiastic about what they're eating when they're involved in the planning and/or prep process.
What we did first was to have a discussion about what goes into a healthy meal and we decided on: one protein, one grain, one vegetable and one fruit per meal. Together, we drew a picture for each food and labeled it (also a great opportunity for writing and spelling practice!). We sorted the foods into cups for each category: proteins, grains, vegetables and fruits. Avery chose which foods she wanted in her lunch for each day of the week and stuck them onto the chart using washi tape.
Once Avery filled in all of the spaces on the chart, she was able to see that she was eating a variety of fruits and vegetables, just by looking at the rainbow of colors in those two columns. This fed into a conversation about the importance of eating a diverse mix of fruits and vegetables every week (not just the same ones every day), so that she would benefit from the different vitamins and minerals that each one provided. We also began to talk a little about the seasonality of fruits and vegetables, which ones were available each season and when they were at their peak. These are all all great conversations to start with children, which will hopefully sow seeds for healthy, mindful patterns of eating in the future as they become more independent and have to make their own choices about what they eat.
Hope everyone's school year is off to a terrific start!
Thank you to The Land of Nod for sending the Easel, Paper Roll & Paint Cups for this post.
You can see Michelle's work and read about her two young children and their first forays in cooking, art and everything in between at Avery and Augustine.


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Hot actors and actresses over 50
Sandra Bullock, John Stamos, Brad Pitt, Sharon Stone and more: These celebrities have sailed into their 50s and beyond looking good.
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New iPod shuffle available worldwide this Friday
Apple today announced that its new second-generation iPod shuffle will be available in Apple retail stores and Apple Authorized Resellers this Friday, November 3rd. Announced at Apple's "Showtime" event last month, the new shuffle is nearly half the size of the original model, weighs half an ounce and features an all-new aluminum design with a built-in clip. The new shuffle, which Apple calls "the world's smallest digital music player," offers 1GB of storage, which holds up to 240 songs, and is priced at $79. We reported yesterday that pre-orders for the shuffle began shipping over the weekend.
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When I was in the Apple store in Toronto yesterday, someone phoned in to ask if they had Shuffles in stock, and a guy told the girl who answered the phone to tell her no, because they're not allowed sell them yet. If they're shipping from online, why not sell them straight away in the shops?
I was planning to get one, but Apple released that fix that unbricks my old 512MB shuffle so I'll stick with that.
Posted by LC Angell on October 31, 2006 at 10:15 AM (CST)
well…just lost my nano, so I guess this will do me for a while. woot.
Posted by LC Angell on October 31, 2006 at 10:22 AM (CST)
Hmmmmmm…I'm guessing it will take 2 to 3 hours before someone sues apple because, "the shuffles design lends itself to easily being lost between seat cushions and deep pockets rendering the product unusable, therefore representing an obvious manufacturing defect ignored by apple computer, inc."
Posted by LC Angell on October 31, 2006 at 10:22 AM (CST)
hilarious!!!...and just might happen. thanks for the laugh out loud.
Posted by LC Angell on October 31, 2006 at 12:12 PM (CST)
The FedEx guy just dropped off my Shuffle. Everything is as expected, though I noticed that the headphones are the old ones, not the new ones shipping with the newest 5G ipods. Not a big deal.
Posted by LC Angell on November 1, 2006 at 10:16 AM (CST)
Two q's.
One, can this thing go back and forth between a Mac (home) and PC (work) or is it gonna bug out?
Two, anyone know of any accessories yet?
B Strange
Posted by LC Angell on November 1, 2006 at 1:42 PM (CST)
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text: AdChoices Cookie Consent Terms of Use Privacy Policy Hide STYLE Fashion Trends Baume & Mercier: The Clifton Series Baume & Mercier Clifton 10053 2/3 Baume & Mercier: The Clifton Series Jeff St. Aubin December 6, 2012 Share Tweet 0 Shares The Clifton 10053 is about as timeless as it gets. With its black, satin-finished dial and brown alligator strap, this model is as understated as it is luxurious. Don't think for a minute that its simple design and lack of ornamentation will detract from its impact. This watch, with its rhodium-plated hands, will draw appreciative glances wherever you find yourself, be it in the boardroom, at the club or on the links. Next Slide Men's Watches Luxury Watches Baume & Mercier Watches Comments Show Comments More Like This Fashion Advice Check Out 2014's Hottest Can't-Miss Watches Read More Fashion Trends The Best Value-For-Dollar Watch On The Market Today Read More Watches Time To Spoil Yourself? Read More Watch Snob® Stop Everything - The Snob Has Found The Holy Grail Of Steel Sports Watches Read More Watch Snob® Are Watch Forums Worth Your Time? Let The Snob Tell You Read More Watch Snob® The Watch Snob Talks Watch "Value" & Omega's Seamaster Read More Around the Web Special Features


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George 'Boomer' Scott, All-Star first baseman, dies at 69
Published July 29, 2013 1:28 pm
This is an archived article that was published on sltrib.com in 2013, and information in the article may be outdated. It is provided only for personal research purposes and may not be reprinted.
Greenville, Miss. • George "Boomer" Scott, a three-time All-Star first baseman during the 1960s and 1970s who slugged 271 career homers, has died. He was 69.
Washington County coroner Methel Johnson confirmed Scott died on Sunday in Greenville.
Scott spent most of his 14-year career with the Boston Red Sox and Milwaukee Brewers. He hit 27 homers during his rookie season with the Red Sox in 1966 and had his best year with the Brewers in 1975, when he hit 36 homers and had 109 RBIs.
Scott was a big man, listed at 6-foot-2 and over 200 pounds in his playing days, but surprising nimble in the field. He won eight Gold Gloves, playing primarily first base.
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Written on: 27/07/2014 by Portera411 (1 review written)
I ordered a bike with the wrong size. The change the bike no problem (even if it had a massive reduction and I rode it once). And they supervised all the process to make sure everything was in order. The costumer service team was really helpful and nice.
Written on: 25/08/2014
Thank you for taking the time to share your experience with us. We are very glad to hear you are happy with the service provided to you on this occasion and we hope you are currently enjoying the exchanged bike received. Should you require any further assistance please let us know, we would be more than happy to help.
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Conference call: Doc to L.A.
The fiasco has ended, as well as an era.
After nine seasons, Doc Rivers has left the Celtics for the Los Angeles Clippers. With his departure, another chapter in Celtics history, where the Green won one NBA championship and came within one game of another, has ended.
The multi-million dollar question: what caused Doc to leave the Celtics to go coach the Clippers?
It has been reported that the primary reason Doc wanted to leave Boston was because of point guard Rajon Rondo, whom he had a rocky relationship, and nearly came to blows with this past season according to NBA writer Chris Sheridan.
"Doc's done a pretty good job of pulling the wool over everyone's eyes on how difficult it is to coach Rajon Rondo," said Sheridan, "and stories have come out that there have been locker room blowups."
For more of what Sheridan had to say, click the video above.


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January 28, 2003
By: Ivo H. Daalder and James M. Lindsay
The UN weapons inspectors' report to the Security Council yesterday emphasized Iraq's continued failure to cooperate fully.
"Iraq appears not to have come to a genuine acceptance, not even today," said chief UN weapons inspector Hans Blix, "of the disarmament which was demanded of it."
More time alone will not resolve the problem. Without Baghdad's active cooperation, even doubling or tripling the number of UN inspectors is unlikely to produce concrete evidence that Iraq possesses weapons of mass destruction or the means to produce them. War now seems inevitable.
In his State of the Union address to the nation tonight, President George W. Bush is sure to make that case. He will argue that 10 weeks ago the United Nations gave Saddam Hussein a final opportunity to rid Iraq of all weapons of mass destruction. Hussein failed to seize his chance to disarm peacefully.
Bush will point to the numerous ways in which Hussein refuses to cooperate with inspectors. As Blix emphasized, important questions remain about Iraq's inventory of anthrax, VX nerve agent, mustard gas and ballistic missiles. Baghdad has concealed crucial documents, refused to allow its scientists to be interviewed and posed unacceptable conditions on aerial reconnaissance inspections of its territory.
But while war now looks inevitable, the question of timing remains open. In view of the heated rhetoric coming out of the White House this last week, some expect the president to order troops into combat in the next few days. But the administration has good reason to delay the start of war for several weeks.
One is that American troops in the region are not yet up to their full battle strength. As Gen. Richard Myers, the chairman of the Joint Chiefs of Staff, said last week, the U.S. military is ready to attack if so ordered. But attacking now, before all the troops are in the field, could mean harder and bloodier fighting than would be the case when U.S. forces reach full strength later next month.
Another reason for delay is that many of our allies are not yet on board. Last week's spitting match across the Atlantic showed the depth of the divisions. Basic differences persist over the extent of the threat and how best to deal with them. There is deep distrust in foreign capitals over what is perceived as the Bush administration's headlong rush to war.
Even key allies like Britain, who want to be with us, may not be if the run up to war is mishandled. British Prime Minister Tony Blair, who meets with President Bush this Friday, insists a new UN resolution authorizing force is needed. Over the weekend he said the only circumstance in which he could envision acting without UN blessing is if inspectors say Iraq is in breach of its obligations and another permanent member of the Security Council "unreasonably exercises their veto and blocks a new resolution."
The growing criticism abroad is undercutting public support at home. Polls consistently show that Americans want the support of other countries. One recent survey found that fewer than three in 10 Americans would support a unilateral U.S. attack.
Equally significant, Americans by a two-to-one ratio favor giving inspectors more time.
With inspections unlikely to force Iraq's disarmament, but with the military still deploying and our allies and the public not yet fully on board for war, what should President Bush do? He could forge ahead alone, gambling that everyone else will follow his lead.
While the public probably would rally around the White House, allied capitals might not. And while Washington can win the war on its own, it needs allied support to win the peace that follows.
Rather than going it alone, Bush should make one last effort to forge a broad international coalition. He can meet his critics half way, and put off an attack until mid-March. This would give Baghdad one last chance to answer the unresolved questions UN inspectors identified in yesterday's report.
In return for giving the inspections more time, the United Nations Security Council would agree to issue an ultimatum: Iraq must resolve every outstanding issue by March 15 or face war.
Given that U.S. troops are not fully deployed, the United States has little to lose and much to gain from such a deal. Accepting a few weeks' delay would answer the criticism that Washington is more interested in war than in disarmament.
Saddam Hussein could still escape his fate if he finally comes clean on his weapons programs, giving some allies hope that war could be avoided. But his failure to do so would leave the allies with no choice but to participate in his ouster.
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Kraft BistroRead MoreTake a trip to Kraft Bistro in Bronxville and make your next meal a good one. For healthy and nutritious meals filled with flavor, dine at Kraft Bistro. Kraft Bistro guests can also take advantage of the many drink options offered here. Guests may have a hard time conversing, as the restaurant is rather noisy. Get to the restaurant early to have your pick of tables — with its no-reservation policy, the place can fill up at busy times. Formal attire is required so that you can look as nice as your meal does. Impress the diners at your next gathering by calling in Kraft Bistro for catering. If you need to get somewhere fast, the restaurant also serves up grub to go. Drivers should plan to park on the street when dining at Kraft Bistro's Kraft Ave residence. Prices are a bit on the higher side, so this might be a good pick for a special night out. Breakfast, lunch, and dinner are all served at the restaurant, but reviewers rate the dinner menu the highest.104 Kraft AveBronxville, NY US
Bistro ParisienRead MoreBorn in Northeastern France, Bistro Parisien founder Stephane Kane returns to his roots every time he steps into the kitchen. Along with his wife Maria, Kane oversees a season-centric menu that Westchester Magazine calls "straight out of a French cookbook" and Lohud.com dubs "phenomenal." This means plenty of French staples, such as steamed mussels with garlic and parsley and coq au vin—chicken stew in red wine sauce with caramelized onions. More than the food, though, Bistro Parisien strikes a chord with diners for its warm, homey atmosphere. Carpeted floors, eclectic plates, and quirky salt-and-pepper shakers give the restaurant the feel of a countryside home, and limited seating helps maintain a cozy intimacy.434 White Plains RoadEastchester, NY US


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Resolution: standard / high
Figure 7.
Graphic representation of differences in the ITS2 region between and within Caenorhabditis species as branch lengths of a tree for 50 Caenorhabditis strains. Branch length was determined by maximum parsimony (see Methods). With one exception (* C. sp. 8), the differences between strains of the same species (blue boxes) are smaller than the smallest differences between the two most closely related species pairs (orange boxes). In all cases, the differences separating any pair of species is much greater than the differences separating strains of the corresponding species. **The differences between C. remanei strains are larger than the differences seen within other species. Recently, however, hybrid breakdown has been observed in matings between strain VX0088 from China and several strains from Ohio, congruent with the long ITS2 branch of the Chinese isolates (Asher Cutter and Alivia Dey, pers. comm).
Kiontke et al. BMC Evolutionary Biology 2011 11:339 doi:10.1186/1471-2148-11-339


text: I stayed in the Intercontinentaladdis for 6 night in August '15 on a business trip. The hotel itself isn't part of the Intercontinental chain, it's independent. It advertises itself as 5*. The hotel will be 5* by Ethiopian standards, but if you're used to the Western world's standard of 5* then you may be disappointed. The hotel itself is clean,... More


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Application provides an access to galleries, which are designed and hosted by customer. This kind of controlling pictures and images is extremely convinievnt.
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Another Sunday morning with Meggan Anderson.
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Uliffany is tackling the big issues this Sunday morning...contemplating the meaning of life, death, & smelly man-balls.
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Josh Mayhood stars in the first episode of his very own talk show, which also happens to be filmed in his apartment.
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Music video. Guy meets girl. Guy loses girl. Guy eatts ice cream
After Last Night: Sunday Morning
The ingredients went something like this: Two guys. Two girls. Cocktails. Hook ups. What could go wrong? (Not much if they used Bedsider and some party-ready...
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Old Man's Apt - E2S1 - Sunday Morning
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The good people at Good Morning America had Jake and Bob on the Sunday edition. We stole some camera's and made their day awesome with high-fives
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Fish peppers: brilliant display of color from an autumn garden - 0 Comments
I picked the last of my fish peppers today. It was a sad day after a very bountiful harvest that began back in July.
Fish peppers are an heirloom hot pepper that were apparently popular in the African-American community in colonial times. I bought the seeds last year in the gift shop at Thomas Jefferson's Monticello, his gorgeous mountain top home in Virginia.


text: We stayed at the W Austin during the very busy Formula 1 Race weekend. The service we got here simply could not have been better. From the excellent concierge who contacted us weeks in advance to the doorman and valet who recognized us as guests amidst the large crowds, everyone here went out of their way to ensure that we were happy. There were special race related gifts waiting in our room, they were happy to accommodate an extra guest and they treated us like rock stars even though we clearly were not! We had a very nice corner suite with beautiful decor, luxury bathroom and a clean comfortable bed. The only teeny tiny thing i didn't like was there was no coffeemaker in the room. I would have loved to have a Keurig in there, but the rest of the experience was so overwhelmingly positive that we will be booking our stay for next year as soon as reservations open up.


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The first thing that struck me about this hotel was that I felt instantly at home. Nothing was a problem for the staff, they were so incredibly friendly and helpful. We had booked a twin room, but had been given a double. The staff promptly changed around beds for us without a hint of confusion or panic, they just did it with a smile!
Breakfast (included in the price) was representative of a traditional Moroccan breakfast, with fruit, yogurt, orange juice, pancakes, and a selection of breads. However, I felt the option of eggs could have been included. We only had dinner here once, and although we were supposed to give a whole day notice, the staff still said they would cook dinner for us even though we had only given a couple of hours notice. We only sampled the tagliatelle bolognese, which was delicious, but we did have better food elsewhere.
There were two things that I felt let the riad down a little bit. Firstly, the furniture on the rooftop terrace seemed a little bit neglected, and the sun loungers were just too dirty and dusty to sit on comfortably. It is understandable that things on the roof would be dusty, but it would have been a good idea to provide blankets to cover them with that could then be easily shaken off and washed. Secondly, the plumbing was a bit noisy, making a series of clunking and droning noises every time someone ran the tap or had a shower, sometimes keeping us awake a night.
Overall, room for improvement, but the staff here helped make our trip to Marrakech enjoyable and made everything run smoothly, letting us use the phone in reception to organise trips, and, on a couple of occasions, escorting us when we weren't sure which way to go! They absolutely went above and beyond great customer service.


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Paying the penalty in a football case
In a recent claim brought against Blackburn Rovers Football Club by Henning Berg, its manager, the High Court decided that a contractual payment due as compensation on early termination of Berg's fixed-term contract by the club was not a penalty and was enforceable.
Blackburn Rovers terminated Berg's three-year fixed-term contract after just 57 days in the job. There was a provision in Berg's contract giving Blackburn Rovers the express right to terminate, provided that the club notified him in writing and paid him a sum equal to his gross basic salary for the unexpired balance of the fixed period (£2.25m) as liquidated damages.
The club initially admitted that it was liable to pay the penalty of £2.25m, but later argued that the contractual provision was a penalty clause and therefore unenforceable. It was in the club's interest for the clause to be deemed a penalty as that would mean that payment under it could not be enforced by Berg, who would then only be entitled to damages for breach of contract under normal contractual principles. That would require him to mitigate his loss, limiting his entitlement to damages by requiring him to take steps to find alternative employment…
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Light Up Purple 2015 – www.lightuppurple.com Facebook – www.facebook.com/lightuppurple
World Mental Health Day (10 October) is a day for global mental health education, awareness and advocacy. The first WMHD was in 1992 at the initiative of the World Federation for Mental Health, a global mental health organization with members and contacts in more than 150 countries. On this day each October – thousands of supporters come to celebrate this annual awareness program to bring attention to Mental Illness and its major effects on peoples' life worldwide.In some countries this day is part of the larger Mental Illness Awareness Week. There is an great need to provide more information on mental wellness.
Light Up Purple with 100 LED solar fairy lights – $15 + shipping.Email to firstname.lastname@example.com for more information on ordering.
Purple battery powered tealights ~~~ $2.00 donation + shipping
To order bracelets, email to – email@example.com
CBC Fifth Estate – (#1) Sextortion of Amanda Todd and (#2) Stalking Amanda Todd: The Man in the Shadows
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For Immediate Release: 8/27/2014
|Maker Faire Coming to Silver Spring on September 14
Montgomery County is again teaming up with KID (Kids International Discovery) Museum to host the Silver Spring Maker Faire on Sunday, September 14 from noon to 5 p.m. at the Silver Spring Civic Building, at the corner of Ellsworth Dr. and Fenton St. in downtown Silver Spring. Last year's Silver Spring Mini Maker Faire was so popular in the DC area that the event has graduated from a Mini Maker Faire to a Maker Faire. The free faire will offer hands-on activities and showcase innovative projects created by inventors, hackers, crafters, artists and "do-it-yourselfers" of all kinds.
The Silver Spring Maker Faire is one of only four featured faires across the country. The goal of the event is to demonstrate creative and unusual pursuits to inspire others to explore their own curiosity and to make something new and different.
Fun and innovative hands-on activities for children and adults will include:
• Walkable robots, robot challenges, FIRST robotic teams and drawbots;
• Wind powered art and robots and giant wind machines;
• Science demonstrations, tesla coils and singing bowls;
• Prototypes of electronic systems made with Little Bits;
• Hovercraft rides;
• 3-D printers and scanners;
• Bi-copters, rockets and other flying machines;
• Felt-making and conductive thread and LED sewing for light-up clothes;
• Solar powered flashlight making;
• Interactive simulations and video games, coding classes, scratch programming, app making and hacking;
• Cardboard playgrounds and recycled art exhibits; and
• NASA's Digital Photo Booth.
The faire also will offer food, two stages of entertainment including a series of short talks from "Inventors in Our Midst.
Sponsors include KID Museum; Montgomery County Government; Make Magazine; HESS Construction; Petrie Ross; Montgomery College; ROBLOX; HEX Bugs; the Tower Companies; Washington Gas; Engineering World Health; Tech Shop; Lerch, Early and Brewer; Wilson, Sonsini, Goodrich and Rosati; ABC7/WJLA-TV; NewsChannel 8; WTOP; Downtown Silver Spring; Activity Rocket; The Gazette; and The Washington Post.
The event is accessible from the Silver Spring Metro Station, and free parking is available in the nearby Town Center and Wayne Avenue garages.
For more information, visit www.makerfairesilverspring.com.
|Release ID: 14-316
Media Contact: Elizabeth Gallauresi 301-439-5100
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Reviews of Ilala Lodge
They do not necessarily represent the views of Expert Africa.
Ilala Lodge review
Lovely hotel and a fitting end to our holiday. We arrivied at about lunch time and after settling in had a light snack . In the afternoon we went on the sundowner which was fantastic lots of animals and birds and drinks and snacks! Our room was on the first floor with doors opening onto a terrace. from where we could see and hear the roar and see the spray of the falls.
Pool area nice and clean. Staff very attentive. Evening meal very good. Next day walked to the falls. No trouble there. Would happily stay again. It is pricey and everything is in american dollars but a lovely lovely hotel.
Ilala Lodge review
Although the facilities are a little timeworn at the Lodge they are of a decent standard and the layout of the communal areas is very pleasant indeed. Food was always nicely presented and perfectly acceptable, and service, whether restaurant, reception or otherwise, was excellent. So, we enjoyed our stay there.
The Falls themselves are, of course, quite stunning; awesome in fact at any rate in late February when the waterflow is high. I deliberately courted a drenching, and duly got soaked to the skin. We went to the Falls in the morning and took the helicopter flight in the afternoon. We are convinced that that is the best way to get the full experience of the Falls. We ate one evening in the Vic Falls Hotel. We dined, satisfactorily, in the restaurant on the terrace. The main restaurant was completely deserted!
As to the town itself, we particularly liked the Elephant's Walk Shopping Village (in which there is a pleasant open-air restaurant at which we had a good light lunch).
A Great Place to End Our Trip
The setting overlooking the spume of The Falls is wonderful. The walk to The Falls was easy and not as pestered by salesmen as we had feared!
The room was comfortable without being luxurious (and a tad noisy at times as they are building an extension to the Lodge!). Bar and restaurant prices were high compared to Namibia. The restaurant setting was lovely and the service excellent.
I don't think they are used to people staying 3 nights as the meat heavy menu did not vary from night to night!
Great for visiting Victoria Falls.
This is a large lodge with many guests, but it is in a perfect position for walking down to the Victoria Falls. It is very comfortable and the a.c. in the rooms was very welcome (especially as the temperature was around 38 deg. C whilst we were there). The breakfasts were lovely with the highlight of churros every day. The evening meals were pretty good too. It was lovely to eat outside.
We did a trip of an evening safari in Livingstone and Stanley Park, which is a private estate where rhino conservation is a success. After a sundowner we were taken to an outside dining area and had a lovely meal arriving back at the hotel at 9 p.m.
Zimbabwe is very expensive after Namibia. They have aligned their currency with the US dollar. The local population was keen to barter with us using any spare clothes or shoes we had as they find it hard to buy these things.
Ilala Lodge review
Well run hotel on the edge of town.
Easy 10 minute stroll to Victoria Falls which has a good trail system with plenty of viewpoints.
Absolutely lovely hotel; most staff very attentive and helpful, although reception staff a bit brisk in their manner sometimes. Gorgeous, very comfortable room with balcony from which you could see the spray from the Falls and hear them too; lovely bathroom. Very good food, including a separate vegetarian menu for dinner which was excellent. Everything was close by. Great to have a pool to chill out in (literally and metaphorically). One of the best hotels I've ever stayed in.
We were concerned when we got there to find out there were building works immediately to the left of our balcony view. Thankfully this stopped soon after we arrived and didn't continue as we were there over the weekend. It should have been mentioned to us in case we felt we needed to move room or even our hotel given that we had specially arranged a suite for my husband's 50th birthday.
Ilala Lodge review
Lovely lodge, great location.
Very convenient for walking to the falls and into the town. Pool and bar area very relaxing, good way to start our holiday
Ilala Lodge review
Great views from our well appointed room. The situation of the Lodge meant that we could easily walk both into the town and to the Falls. Though we walked through the impressive Victoria Falls hotel, we would not have swopped!
News that this was our ruby wedding celebration had obviously been passed on (thank you Nick) and we were greeted with champagne and flowers in our room - both much appreciated.
Ilala Lodge review
Great location, really close to the FallsRead more about the whole trip
Ilala Lodge review
Ilala Lodge is a beautiful lodge set within walking distance of Victoria Falls. We were given what we believe must have been the best room at the lodge and for that we thank you very much, Claire and Nick! Any time not spent at The Falls, the town, on a cultural tour, on a river cruise or eating and/or drinking in the lodge's lovely dining room or bar was spent on our terrace. I cannot imagine a better way to start our trip. The staff was most helpful and the people who arranged our activities were knowledgeable and eager to chat with us about the various activities open to us. The best tour for us was the cultural tour. Our guide took us to the homestead of Crispen and we learned so much. It was a major highlight of our trip. We also visited the local school. The children are beautiful, polite, friendly, happy, beautifully groomed in crisp uniforms. It hurts that they have so few classrooms available to them and can only attend school for half days because of the number of children and lack of space.
Information on how to avoid the curio touts would be most helpful.
I would follow Nick's advice and wait to arrange any tours after arriving at the lodge. I had thought of doing it ahead of time but, as Nick suggested, there was no need.
The only complaint we have is that the dinners were not good at all. We did not eat our main course the first two nights because the beef on the first night had a strange, unappealing taste. The vegetarian curry dish the following night was too spicy to eat - and we usually enjoy food made with curry. Just too spicy hot to eat. At our first camp, the manager told us Ilala Lodge had recently lost its chef which seems the most likely cause for the poor food. But the breakfasts were outstanding!
Because it is pitch black at night and because there are so many men along the walkways trying to get us to buy things from them, we were too uncomfortable to leave the lodge at night to find a different spot to eat.


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Hackett Catholic Central won their third D4 MHSAA championship in the past five seasons.
Created on June 15, 2013
Kalamazoo Christian topped Ubly 16-6 in six innings to win the Michigan state division 4 softball championship title.
Created on June 15, 2013
Mattawan topped Bay City Western 2-1 to win the Michigan state division 1 softball championship title.
Created on June 14, 2013
Mattawan softball defeated Romeo 3-2 in the Michigan Division 1 state semifinal game in Battle Creek.
Created on June 14, 2013
Kalamazoo Christian won the Division 4 state tournament semifinal game 2-0 against Rogers City at Battle Creek.
Created on June 6, 2013
Hackett Central Catholic won the D4 boys' golf regional tournament at Millham Park Golf Club.
Created on June 4, 2013
Kalamazoo Christian girls' soccer won their regional tournament game against Lake Michigan Catholic.
Created on May 31, 2013
Kalamazoo Christian softball beat Climax-Scotts 8-1 to win the D4 district championship at Climax-Scotts.
Created on May 31, 2013
Kalamazoo Christian baseball beat Bellvue 11-1 to win the D4 district championship at Climax-Scotts.
Created on May 30, 2013
Schools from around the region teed off for the D2 boys' golf district tournament.


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Iona March Madness
Iona's basketball players and hundreds of their supporters gathered at Hynes Athletic Center for the unveiling of the brackets on the NCAA's "Selection Sunday" show. Iona was seeded 15th and was paired against No. 2 seed Ohio State in the NCAA Tournament's West Region. (March 17, 2013)
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