text:
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Published online before print 29 April 2009.
DOI 10.1095/biolreprod.108.075648
Constitutive Activation of Beta-Catenin in Uterine Stroma and Smooth Muscle Leads
to the Development of Mesenchymal Tumors in Mice1
Pradeep S. Tanwar,3Ho-Joon Lee,3LiHua Zhang,3Lawrence R. Zukerberg,4Makoto M. Taketo,5
Bo R. Rueda,3and Jose M. Teixeira2,3
Vincent Center for Reproductive Biology, Vincent Obstetrics and Gynecology Services,3and Department of Pathology,4
Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts
Department of Pharmacology,5Graduate School of Medicine, Kyoto University, Japan
Leiomyomas and other mesenchymally derived tumors are the
most common neoplasms of the female reproductive tract.
Presently, very little is known about the etiology and progression
of these tumors, which are the primary indication for hysterec-
tomies. Dysregulated WNT signaling through beta-catenin is a
well-established mechanism for tumorigenesis. We have devel-
oped a mouse model that expresses constitutively activated beta-
catenin in uterine mesenchyme driven by the expression of Cre
recombinase knocked into the Mu ¨llerian-inhibiting substance
type II receptor promoter locus to investigate its effects on
uterine endometrial stroma and myometrium. These mice show
myometrial hyperplasia and develop mesenchymal tumors with
100% penetrance that exhibit histological and molecular
characteristics of human leiomyomas and endometrial stromal
sarcomas. By immunohistochemistry, we also show that both
transforming growth factor beta and the mammalian target of
rapamycin are induced by constitutive activation of beta-
catenin. The prevalence of the tumors was greater in multipa-
rous mice, suggesting that their development may be a
hormonally driven process or that changes in uterine morphol-
ogy during pregnancy and after parturition induce injury and
repair mechanisms that stimulate tumorigenesis from stem/
progenitor cells, which normally do not express constitutively
activated beta-catenin. Additionally, adenomyosis and endome-
trial gland hyperplasia were occasionally observed in some mice.
These results show evidence suggesting that dysregulated,
stromal, and myometrial WNT/beta-catenin signaling has
pleiotropic effects on uterine function and tumorigenesis.
adenomyosis, beta-catenin, developmental biology, endometrial
stromal sarcoma, hypertrophic scarring, leiomyoma, signal
transduction, uterine fibroids, uterus, WNT signaling
Mesenchymal uterine tumors are the most common
neoplasms found in the human female reproductive tract. The
vast majority of these tumors are uterine leiomyomas (uterine
fibroids), which are benign lesions that form in the myome-
trium of an estimated 25%–50% of American women. Uterine
leiomyomas are clonal in origin and hormone responsive [1].
Patients may have a single uterine fibroid, but many have
multiple fibroids, and they can be located anywhere in
myometrial tissue. These tumors are commonly associated
with severe pelvic discomfort during menstruation (dysmenor-
rhea), heavy menstrual bleeding (menorrhagia), infertility,
abortion, ascites, and polycythemia. On the basis of their
location in the uterus, fibroids are subdivided into three broad
categories: subserosal, submucosal, and intramural. The size of
the fibroids can vary greatly from microscopic to tumors
weighing more than 1 kg, are often associated with significant
morbidities, and are the leading cause of hysterectomies [2].
Other uterine tumors such as leiomyosarcomas, endometrial
stromal sarcomas (ESSs), perivascular epitheliod cell tumors
(PEComa), and adenocarcinomas are less common but still
pose a significant burden on a women's health and are often
fatal. The etiology of any of these mesenchymal tumors is
largely unknown.
One of the major mechanisms of neoplastic transformation is
dysregulated b-catenin signaling. b-Catenin is the core
component of the canonical wingless-type MMTV integration
site family member (WNT) signaling pathway whose activation
leads to the translocation of b-catenin from the cytoplasm to the
nucleus and transcription of targeted genes [3]. The role of
WNT signaling in carcinogenesis in other tissues is well
documented [4], but evidence for its contribution to mesenchy-
mal uterine tumor development has been limited [5–7]. WNT
signaling is indispensible for normal uterine development from
the primordial Mu ¨llerian duct [8–10], and there is accumulating
evidence to support a role for WNT signaling in postnatal
uterine functions, including implantation [11], decidualization
[12, 13], and perhaps remodeling after parturition.
We have been investigating b-catenin signaling in uterine
development and function using a mouse Cre recombinase
model system [14, 15] that exploits the uterine mesenchyme-
specific expression of the Mu ¨llerian inhibiting substance type
II receptor, which is also known as anti-Mu ¨llerian hormone 2
(AMHR2). We have shown that conditional deletion of
b-catenin in uterine mesenchyme leads to progressive cell fate
change from myogenesis to an adipogenic lineage [15]. The
aim of the present study was to investigate the role of b-catenin
in uterine development further by expressing a constitutively
activated form of b-catenin in uterine mesenchyme driven by
Amhr2-Cre. When Amhr2tm3(cre)Bhr(Amhr2-Cre) mice are
crossed with Ctnnb1tm1Mmtmice [16], Amhr2-driven Cre
recombinase, which is expressed in ovary, oviduct, and uterus,
deletes exon3 and leads to formation of a stable form of
b-catenin in Amhr2-Cre-expressing cells and in their progeny.
1Supported by a grant from the National Institute of Child Health and
Human Development, HD052701, to J.M.T.
2Correspondence: Jose M. Teixeira, Vincent Center of Reproductive
Biology/Thier 913 Massachusetts General Hospital and Harvard
Medical School, 55 Fruit St., Boston, MA 02114. FAX: 617 726 0561;
e-mail: firstname.lastname@example.com
Received: 15 December 2008.
First decision: 12 January 2009.
Accepted: 9 April 2009.
? 2009 by the Society for the Study of Reproduction, Inc.
This is an Open Access article, freely available through Biology of
Reproduction's Authors' Choice option.
eISSN: 1259-7268 http://www.biolreprod.org
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This form of b-catenin is resistant to phosphorylation by the
adenomatosis polyposis coli complex and its subsequent
degradation. We show that dysregulated WNT signaling in
the uteri of these mice causes mesenchymal tumorigenesis in
all mice examined and occasional adenomyosis, with probable
indirect stromal support for the induction of adenocarcinoma.
Mouse Husbandry
The mice used in this study were housed under standard animal housing
conditions. All protocols involving animal experimentation were approved by
the Institutional Animal Care and Use Committee at Massachusetts General
Hospital. Mice were maintained on C57BL/6;129/SvEv mixed genetic
background. Amhr2tm3(cre)Bhr(Amhr2-Cre) mice [17] (Dr. Richard Behringer,
M.D. Anderson, Houston, TX) were mated with Ctnnb1tm1Mmt/tm1Mmt[16] to
generate Amhr2tm3(cre)Bhr/þ;Ctnnb1tm1Mmt/þmice. The DNA from tail biopsies
was used to genotype mice using the standard PCR protocols. The uterus
collected from Amhr2tm3(cre)Bhr/þ;Ctnnb1tm1Mmt/þ and control mice were
photographed by using a Nikon SMZ1500 microscope with an attached Spot
camera (Diagnostic Instruments, Sterling Heights, MI).
Oocyte and Blastocyst Collection and Assessment
The 6- to 7-wk-old mutant and control mice were superovulated with i.p.
injections of 7.5 IU equine chorionic gonadotropin (eCG; Sigma Chemical Co.,
St. Louis, MO) followed by 7.5 IU human chorionic gonadotropin (hCG;
Sigma) after 48 h. Fourteen hours after hCG injection, females were euthanized,
and their oviducts were removed. The cumulus-oocyte complexes were released
from the ampullary region of each oviduct by puncturing the oviduct. Cumulus
cells were removed by exposure to 80 IU/ml hyaluronidase (Irvine Scientific,
Santa Ana, CA) in Hepes buffer and washed out with human tubal fluid (HTF;
Irvine Scientific) with 10% fetal bovine serum (FBS). Isolated oocytes were
transferred into HTF media with 10% FBS and assessed by morphology.
Oocytes were classified into four groups as mature, germinal vesicle break
down, immature, and atretic. For blastocysts, the 6- to 7-wk-old mutant and
control mice were superovulated with 7.5 IU eCG and 7.5 IU hCG as above then
mated with wild-type males. Mice were euthanized at 3.5 days after mating, and
uteri were removed. Blastocysts were flushed from the uteri and transferred into
HTF media with 10% FBS and assessed by morphology. Blastocysts were
classified as 4-cell to morula, expanded blastocyst, and early blastocyst.
Human myometrial and fibroid samples were collected using Institutional
immersion in 4% paraformaldehyde for 10–12 h and then transferred to 70%
ethanol until processing. The fixed tissues were dehydrated in a graded ethanol
were sectioned at 5-lm thickness and mounted on slides. Hematoxylin and eosin
(H&E) staining was performed using standard histological techniques. Photomi-
crographs of nontumorous myometria (n¼3 mice for each group) were measured
to determine the ratio of the width of myometria to total uterine width.
In Situ Hybridization
Analyses for Amhr2 mRNA are described in a detail in a previous study
[14, 15]. Briefly, urogenital ridges were collected from timed pregnant dams
and at Embryonic Day 13.5 (E13.5) and fixed overnight at 48C in 4%
paraformaldehyde. Tissues were pretreated with protease prior to overnight
hybridization with either Amhr2 full-length [18] digoxigenin-labeled antisense
riboprobes in 50% formamide hybridization solution at 708C. The resultant
hybrids were detected with alkaline phosphatase-conjugated antidigoxigenin
antibodies and BM purple colorimetric solution (Roche Molecular Biochem-
icals, Indianapolis, IN).
Serial sections of tissues were deparaffinized with xylene and rehydrated
with graded series of ethanol (absolute, 95%, 80%, and 50%, respectively, and
distilled water), followed by two washes of 5 min each in PBS containing
0.05% Tween 20 (PBS-T). After a wash with PBS-T, antigen retrieval was
performed by boiling the tissue sections in 0.01 M citrate buffer (pH 6) for 20
min. Sections were then washed for 5 min in PBS-T and blocked at room
temperature for 1 h by using 2% normal donkey or goat serum, 2% bovine
serum albumin, and 0.1% triton-X in PBS. Tissue sections were then incubated
in a humidified chamber overnight at 48C with primary antibody. Sections were
subsequently washed with PBS-T, incubated at room temperature for 1 h with
secondary antibody, then counterstained with 40,60-diamidino-2-phenylindole
(DAPI). Images were photographed using a microscope (Nikon Eclipse TE
2000-S; Micro Video Instruments, Avon, MA) equipped with a Spot digital
camera. DAPI-stained nuclei and phospho-histone H3 (pH3)-positive cells from
sections of three different lesions were counted with the nucleus-counting
analysis plugin of Image J software (v1.37; NIH, Bethesda, MD) after setting a
black and white threshold at 27–30 in Photoshop (v10; Adobe Systems Inc.,
San Jose, CA).
Following are the primary and secondary antibodies used in this study: anti-
b-catenin (1:250; BD Transduction Laboratories, San Jose, CA); anti-KIT
(1:200; R&D Systems, Minneapolis, MN); anti-TGFB3 (1:200; Abcam,
Cambridge, MA); anti-HMGA2 and anti-PECAM1 (1:200; Santa Cruz
Biotechnology, Santa Cruz, CA); anti-vimentin (1:200; Santa Cruz Biotechnol-
ogy); anti-Ecadherin (1:100; Santa Cruz Biotechnology); anti-pH3 (1:200; BD
Transduction Laboratories); anti-CD11B (1:100; eBiosciences, San Diego, CA);
anti-CD140B (1:200; Ebiosciences); anti-ACTA2, CY3-conjugated (Sigma);
anti- PCNA (ready to use; Zymed, San Francisco, CA); anti-FRAP1(1:200; Cell
Signaling, Danvers, MA); AlexaFluor secondary antibodies (1:500; Invitrogen,
Carlsbad, CA); and biotinylated donkey anti-mouse or anti-rabbit antibody
F(ab)2(1:1000; Jackson ImmunoResearch Laboratories, West Grove, PA).
A previous study has shown that despite normal ovarian
functions, Amhr2tm3(cre)Bhr/þ;Ctnnb1tm1Mmt/þ mutant mice
were subfertile [19]. In our study we also observed that
Amhr2tm3(cre)Bhr/þ;Ctnnb1tm1Mmt/þmice have smaller litters in
younger mice despite their having histologically normal
ovaries (data not shown). Further analyses showed that neither
oocyte nor 3-day-old embryo quality appeared to be signifi-
cantly affected by constitutive activation of b-catenin in the
ovary (Table 1). However, fewer oocytes and embryos were
collected from the mutant uteri (Table 1), which is likely in part
because of structural blockage we observed in some of the
mutant oviducts (data not shown). We next analyzed the uteri
to determine if the subfertility in mutant mice might also be
due, in part, to defective development in mutant uteri. Below
we show evidence that expression of constitutively activated b-
catenin in mouse uteri results in the appearance of two types of
mesenchymal tumors that resemble human leiomyomas and
ESSs (Table 2).
Leiomyoma-Like Lesions
Amhr2tm3(cre)Bhr/þ;Ctnnb1tm1Mmt/þ mutant and
Amhr2tm3(cre)Bhr/þ or Ctnnb1tm1Mmt/þ control mice were
Ctnnb1tm1Mmt/þ mice.*
Oocyte and embryo analysis in Amhr2tm3(cre)Bhr/þ;
Stage Control
27.7 6 9.1 (75%)
4.0 6 2.7 (11%)
0 (0%)
4.7 6 2.5 (13%)
36.3 6 7.7
20.0 6 7.0 (77%)
2.0 6 0 (8%)
0.3 (1%)
3.7 6 1.5 (14%)
26.0 6 7.8Total
Four-cell to morula
Early blastocyst
Expanded blastocyst
6 6 2 (21%)
5.3 6 2 (19%)
16.7 6 3.8 (60%)
28 6 2.0
3 6 1 (19%)
3.7 6 1.2 (24%)
9 6 2.0 (57%)
15.7 6 3.2
* n ¼ 3; mean 6 SD (% of total).
?Two out of six oviducts were blocked in mutant mice.
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examined for phenotypic analysis. Initial gross examination of
adult nulliparous mutant uteri occasionally showed hemor-
rhagic areas (data not shown) along both horns and an
extraneous layer of tissue with protruding nodules at the
anti-mesometrial side of the uteri (Fig. 1A). Examination of
mutant uteri from multiparous mice revealed the presence of
large tumorous growths and consistent multiple hemorrhagic
sites on the surface of the uteri (Fig. 1B). No abnormalities
were detected in the uteri collected from control mice (Fig.
1C). Histological examination of noncycling 4-wk-old uteri
showed that the nodules observed on the surface of uteri appear
to originate from the longitudinal myometrial layer of the
uterus (shown with a red arrowhead in Fig. 1D). We also
observed a small area of dysplasia (shown with a black
arrowhead and outlined in white) in the mutant uteri, which
otherwise appear similar to controls (Fig. 1E), suggesting that
tumorigenesis starts before the mice begin estrous cycling.
Staining of the mutant and control uteri for a-smooth muscle
actin (ACTA2) shows that the polyps are derived from the
myometrium and that there was expansion of the myometrium
in the mutants (Fig. 1, F and G). The ratio of the width of
ACTA2-positive myometrium to total uterine width in the
mutant (0.45 6 0.04, n ¼ 3) was significantly higher (P ¼
0.009) than that of control uteri (0.22 6 0.03, n¼3) suggesting
smooth muscle hyperplasia. Normal WNT signaling is highly
active at the coelomic epithelium of the Mu ¨llerian duct [20].
The nodules we observe in the mutant mice may be the result
of constitutively activated b-catenin on the coelomic epitheli-
um and adjacent mesenchyme of the presumptive anti-meso-
metrial side of the E13.5 urogenital ridge where Amhr2 is
highly expressed (Fig. 1H).
In mutant mouse uteri, we observed dysplastic lesions in
myometria with 100% penetrance that appeared to increase in
size and number with age and parity (Fig. 2, A and B, and
Table 2). The mutant murine tumors were composed of plump
spindle cells surrounded by an extracellular matrix that formed
fascicles (Fig. 2C) that are remarkably similar to those typically
found in human uterine leiomyomas [2] (Fig. 2D). The largest
tumors were often necrotic with hemorrhagia and fibrosis (data
not shown). The murine smooth muscle tumors are ACTA2-
positive (Fig. 2, E–G), which is also characteristic of human
uterine leiomyomas (Fig. 2H). Typically, human uterine
leiomyomas are very slow-dividing tumors and are usually
present with a low proliferative index (0–2 mitoses per 10 high
power field) [21]. We analyzed proliferation in the uterine
smooth muscle tumors observed in mutant mice by performing
pH3 expression analysis and found few pH3-positive cells (5–6
per high-powered filed or 2.4 6 0.2/1000 cells) in these
tumors, whereas pH3 expression was high in luminal
epithelium (LE; Fig. 2, I and J). Proliferating cell nuclear
antigen (PCNA) expression was also analyzed with similar
results (data not shown). These data suggest that, similar to
human uterine leiomyomas and unlike most other more
aggressive tumors, these mouse uterine smooth muscle tumors
also have a low mitotic activity. Another characteristic of many
human uterine leiomyomas is that leiomyomas express higher
levels of TGFB, particularly TGFB3, than the surrounding
myometrium [22–26]. Analysis of TGFB3 in the uteri of
mutant mice showed much higher levels of expression by
immunohistochemistry in the epithelium and endometrial
stroma, as well as in the central areas of the leiomyoma-like
lesions, than in surrounding areas and the remaining myome-
trium and at levels consistent with those observed in human
leiomyoma (Fig. 2, K and L). Together with the appearance of
polyps shown above, these results suggest that constitutively
TABLE 2. Incidence of mesenchymal tumors in Amhr2tm3(cre)Bhr/þ;Ctnnb1tm1Mmt/þ mice.
Age of micePhenotype observed
No. of animals examined
per phenotype
No. of animals with tumorous
phenotype (%)
,6 wk (nulliparous) Smooth muscle tumor and hyperplasia
Endometrial stromal sarcoma-like lesions
Smooth muscle tumor and hyperplasia
Endometrial stromal sarcoma-like lesions
Smooth muscle tumor and hyperplasia
Endometrial stromal sarcoma-like lesions
5 (100)
5 (100)
5 (100)
5 (100)
14 (100)
14 (100)
2 (14.28)
?8 wk (nulliparous)
?12 wk (multiparous)14
Gross uterus from Amhr2tm3(cre)Bhr;Ctnnb1tm1Mmt/þ nulliparous mouse
showing abnormal growth (black and red arrowheads) on the anti-
mesometrial side of the uterus. Nodules were also observed on the surface
of the uterus (black arrowheads). B) Gross uterus from multiparous adult
Amhr2tm3(cre)Bhr;Ctnnb1tm1Mmt/þmouse showing tumorous growth (black
arrowhead) and multiple hemorrhagic sites (black arrows). C) Normal
uterus from a control Amhr2tm3(cre)Bhr/þmouse. H&E-stained section of 4-
wk-old mutant uterus (D) showing polyp-like growth protruding from the
myometrium on the anti-mesometrial surface of the uterus (red
arrowhead) and ESS-like lesions in the endometrial stroma (black
arrowhead) and control uterus (E). ACTA2-immunostained longitudinal
section of a nulliparous mutant uterus (F) with positively stained polyps
(white arrowhead) and control (G). H) In situ hybridization in the E13.5
urogenital ridge shows that the Amhr2 mRNA is expressed in the coelomic
epithelium and subjacent mesenchyme of the Mu ¨llerian duct (outlined in
red with the Wolffian duct). CE, coelomic epithelium; MD, Mu ¨llerian
duct; WD, Wolffian duct; O, ovary; LE, luminal epithelium; ES,
endometrial stroma; M, myometrium. Bars ¼ 2 mm (A–C), 50 lm (D–H).
Constitutive activation of b-catenin in uterine mesenchyme. A)
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Page 4
activated b-catenin induces smooth muscle hyperplasia, which
can then lead to smooth muscle tumor development.
The oncogenic serine/threonine kinase, mammalian Target
of Rapamycin (FRAP1), which lies downstream of the PI3K
signaling pathway and is a master regulator of cell proliferation
[27], is up-regulated in the Eker rat leiomyoma model because
of a mutation in tuberous sclerosis 2 (Tsc2) [28]. We assayed
whether FRAP1 is also induced in these tumors by constitu-
tively activated b-catenin using immunohistochemistry (Fig.
3). In the mutant with CA b-catenin, FRAP1 appears highly
expressed in the central portion of the leiomyoma-like lesion,
with little expression observed in the surrounding hypocellular
areas (Fig. 3A). Relatively strong FRAP1 expression was also
observed in both the circular and longitudinal myometrial
layers (Fig. 3B). In the normal myometrium of control mice,
FRAP1 expression appears to be limited to the endothelial cells
or pericytes (Fig. 3, C and D).
ESS-Like Lesions
In addition to uterine smooth muscle tumors,
Amhr2tm3(cre)Bhr/þ;Ctnnb1tm1Mmt/þ mutants also developed
ESS-like neoplastic lesions that were restricted to the
endometrial stroma (ES; Fig. 4A). These lesions were
ACTA2-negative (Fig. 4B), which we used as a marker to
distinguish between ESS and ACTA2-positive leiomyomas.
These tumors expressed high levels of nuclear b-catenin,
indicating their origin from Amhr2-Cre-expressing mesenchy-
mal cells (Fig. 4C). As expected, only membranous b-catenin
is detected in LE and endometrial glands (EG) of both mutant
(Fig. 4C) and control uterus (Fig. 4D), since Amhr2 is not
expressed in these cells [15]. Some cells at the junction of the
longitudinal and circular smooth muscle layers also expressed
nuclear b-catenin (Fig. 4D). The ESS-like tumors expressed
vimentin, a mesenchymal marker, suggesting the mesenchymal
origin of these tumors (Fig. 4E). Consistent with a previous
study [29], vimentin is also expressed in ES and blood vessels
of both the mutant and control uteri (Fig. 4F).
Further analysis of the ESS-like neoplastic lesions with
various markers used in the diagnosis of human tumors was
performed to determine how well the murine tumors compare.
(B) sections of mutant uteri showing the smooth muscle tumor (outlined with white line) in the myometrium. H&E-stained section of mutant uterus (C) at
higher magnification showing tumorous growth (outlined in white in B) and human leiomyoma (D). Serial sections of mutant uteri in A and B are shown
stained with ACTA2 antibody in E and F. G) Higher magnification image of neoplastic area from F showing that most of the tumorous cells are positive for
ACTA2. H) Representative section of a human uterine smooth muscle tumor showing the typical staining pattern with the ACTA2 antibody.
Phosphohistone staining of tumor area in a mutant uterus is shown in I and at higher magnification with white arrows in J. K and L) TGFB3
immunostaining in the central area of a mutant smooth muscle tumor and a human leiomyoma, respectively. Nuclei are stained with DAPI or
hematoxylin. LE, luminal epithelium; ES, endometrial stroma; M, myometrium. Bar ¼ 50 lm.
Amhr2tm3(cre)Bhr;Ctnnb1tm1Mmt/þ mutant mice develop smooth muscle tumors of uterine myometrium. H&E-stained cross (A) and longitudinal
FRAP1 expression was detected by immunohistochemistry at high levels
in the central areas of a leiomyoma (outlined in white) in a mutant uterus,
the epithelial cells, the ES as well as in the circular myometrial layer (MC)
and longitudinal myometrial layer (ML). B) A higher magnification view of
FRAP1 expression in the MC and ML of a mutant mouse. C) In control
uteri, strong FRAP1 expression is found in the EG, LE, and ES. D)
Expression in the control myometrium appears limited to the endothelial
cells. LE, luminal epithelium; ES, endometrial stroma; M, myometrium;
EG, endometrial glands. Bars ¼ 50 lm (A and C), 100 lm (B and D).
FRAP1 expression in Amhr2tm3(cre)Bhr;Ctnnb1tm1Mmtuteri. A)
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Page 5
The ESS-like neoplastic growths observed in mutant uterus are
strongly estrogen receptor alpha (ESR1) positive (Fig. 5A),
suggesting that these tumors can be responsive to changes in
the levels of estrogen. KIT (CD117) is the tyrosine kinase
receptor for the stem cell factor that has been shown to be
expressed at relatively high levels in uterine sarcomas and
mesenchyme-derived tumors of the uterus [30, 31]. We also
find high levels of KIT expression in the ESS-like tumors (Fig.
5B). Co-staining with ITGAM (CD11B), a monocyte/macro-
phage cell marker, was performed to ensure that the KITþcells
were not hematopoietic cells that had infiltrated the lesion. In
the rest of the endometrial stroma, KIT is only expressed in a
few cells, probably of hematopoietic origin. In addition, these
tumorous lesions do not express PECAM1, an endothelial cell
marker, except for the endothelial cells of blood vessels present
inside the tumorous area (Fig. 5C). HMGA2 is a member of the
high mobility group (HMG) of proteins. The disruption and
misexpression of HMGA2 has been reported in various benign
mesenchymal tumors such as lipomas, uterine leiomyomas,
endometrial polyps, and salivary gland adenomas [32, 33]. In
mutant uteri, we detected very high expression of HMGA2 in
ESS-like tumor lesions compared with the normal adjacent
tissue (Fig. 5D). Although E-cadherin, an epithelial cell
marker, and PDGFRB (CD140B), a fibroblast marker, were
expressed in normal LE and a few scattered cells of ES and
myometrium, respectively, they were not expressed in ESS-like
tumors of mutant uteri (Fig. 5, E and F). These results suggest
that the ESS-like tumors in Amhr2tm3(cre)Bhr/þ;Ctnnb1tm1Mmt/þ
mice strongly resemble ESS tumors found in human uteri both
by histology and by marker profile.
The mutations in phosphorylation sites on exon 3 of the b-
catenin gene have been shown to occur in various tumors [34,
35]. In humans, 38% of cases of endometrial carcinoma show
nuclear and/or cytoplasmic accumulation of b-catenin, sug-
gesting that b-catenin plays an important role in the
development of this disease [36]. In the present study, we
observed hyperplasia and pseudostratification of epithelia of
EGs in 14% of the mutant uteri (Table 2). We also observed
aberrant EGs in the myometrium of the mutant uteri (Fig. 6A).
The colocalization of ACTA2 and E-cadherin further confirms
the presence of EGs in the myometrial layer of the mutant uteri
(Fig. 6B). These phenotypic changes observed in EGs were
surprising because Amhr2-Cre is only expressed in the stromal
and myometrial compartments of the uterus [15], and the
activation of b-catenin only occurred in these two compart-
ments (Fig. 2). However, correctly functioning uterine stroma
is required for normal adenogenesis [10], and our previous
study has shown that conditional deletion of b-catenin from ES
and the myometrium leads to fewer EGs [15]. Collectively, our
white dashed line in all the panels, were analyzed by immunofluores-
cence for various markers. The tumors were ESR1-positive (A), KIT-positive
(B), and were negative for the pan-hematopoietic marker ITGAM (CD11B)
and the endothelial marker PECAM1 (C). The ESS-like lesions were also
positive for HMGA2 (D), and negative for E-cadherin (CDH1) (E) and
PDGFRB (CD140B) (F). Nuclei are shown stained with DAPI. LE, Luminal
epithelium; ES, Endometrial stroma; M, Myometrium. Bars ¼ 50 lm.
Sections of the mutant uteri with ESS-like tumors, outlined in a
like tumors in mouse uteri. A) H&E-stained section of mutant uterus.
Neoplastic lesions are seen in endometrial stroma (outlined in white). B)
ESS-like neoplastic growths are ACTA2-negative. The dotted lines indicate
the location of ESS lesions detected on a serial section by H&E. C) Both
cytoplasmic and nuclear staining for b-catenin were observed in tumorous
lesions (outlined by white dotted line) and normal-looking myometrium of
mutant uterus. Membranous staining for b-catenin is present in the LE and
EG of mutant uteri and in control mice (D). E) The ESS-like tumors were
vimentin-positive but in a pattern that was different from that observed
normally in stromal areas of mutants and in control stroma (F). Nuclei are
stained with DAPI. LE, luminal epithelium; ES, endometrial stroma; M,
myometrium. Bar ¼ 50 lm.
Constitutive activation of b-catenin causes development of ESS-
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text:
Tumbledown Teams Should Heed the Colts
By Thomas George
Published: September 25, 1996
INDIANAPOLIS— Take solace, Jets. Look upward, Saints. Peek over here, Bucs. You are winless in four games. You are being trampled. Respect? Every National Football League team is glancing over its schedule hoping you are on it -- three or four times, please. Your team is the brunt of ugly jokes. Your franchise is being snickered at by your peers.
Well, there is hope. If you look at what happened here on Monday night, what has happened here over the last two seasons, there is certainly hope for every downtrodden team and franchise that has lost its way.
Because if the Indianapolis Colts can do it, any team can do it. These guys were at the bottom of the ladder. No, they were beneath the bottom of the ladder. No, they were beneath the bottom of the bottom.
Look at the Colts five years ago: They were 1-15 then. Three years ago? They were 4-12. They could not block or tackle or catch or make big plays. They could not come back from a deficit. They could not sustain a lead. They lacked confidence and their preparation left too many stones uncovered. They played before home crowds of as few as 38,000.
Jets, Saints, Bucs? Does any of this sound familiar?
Then the Colts began to turn things upside down. Last year they swept Miami and beat San Francisco. They beat the team with the N.F.L.'s best record -- Kansas City -- in a road playoff game. Last year the Colts made it all the way to the American Football Conference championship game, coming within a whisker of the Super Bowl.
This year they are 4-0.
They have beaten the world champion Cowboys at Dallas. On Monday night, they played host to the Dolphins, the then-unbeaten, Jimmy Johnson-coached, full-of-swagger Dolphins, and played punishing, hit-till-you-drop football. It was smash-mouth football, and it was so physical that Miami quarterback Dan Marino could not survive. He left early with a fractured right ankle, but the Colts lost two starting linebackers to injury, too. It was a 10-6 Colts victory and the running game told the story: 28 yards rushing for Miami, 171 for the Colts. Fifteen Miami rushing attempts and 32 for the Colts.
That's back-alley football. The Colts can run the score up on you, and they can play these kinds of close, defensive, physical struggles.
They are riding a high. They have beaten Miami four in a row. They have won 10 of their last 12 division games. They have risen from ashes.
As their coach, Lindy Infante, says: ''It's a different hat we're wearing now.''
''I used to go to high school football games around town and I would get no respect,'' said cornerback Jason Belser, in his fifth year here now, so he saw 1-15 and 4-12. ''The kids would say: 'You play for the Colts? So what. They are sorry.' It hurt. When you look at where we were five years ago. , I never thought this organization would experience the feeling we did here on Monday night.''
The largest crowd ever at the RCA Dome -- 60,891 -- wanted to be associated with a winner here on Monday night. ''As we came out of the tunnel and we could look and see the crowd waving those white towels and going nuts, I tapped a couple of guys,'' Belser said. ''I told them, 'Look, this is what we've been waiting for. This is the kind of support we've been asking for. Let's go out there and do something about it!' And we did.''
So, Jets, Saints and Bucs. How was it done? Here is what the Colts say.
They brought in Bill Tobin as the football man. Personnel is in Tobin's hands. Gotta have a football guy who can swing the ax and gather the right players and coaches. Tobin arrived in January of 1994. Only 12 players are left who were here then.
Gotta have a head coach who hires strong assistants and who either motivates his players to another level or offers some type of leadership that gives you an edge. The Colts' head coach, Infante, especially does the latter with his superior offensive play calling during a game.
''Here's the key with Lindy,'' Colts quarterback Jim Harbaugh said. ''He sees the opponent's whole defense, and he identifies the vulnerable part and he exploits it. And he is not afraid to try new things to exploit it. If he sees the weak spot and we don't have a play that he thinks takes full advantage of it, he will make one up right on the sideline. And, again, he will call it. That takes a lot of trust in your abilities.''
That's another thing the Colts have. Trust. ''I don't exactly know when it happened or how it happened but at some point here last season the players and the coaches began to trust one another,'' Harbaugh said. ''And out of that was born a belief. We believe that we can beat any team, and that we will never quit.''
The Colts built something new. ''You have to have good players that play good,'' Infante said. ''You need a quarterback that plays well and you need a solid offensive line. You need people who can make plays. You need a good defense that keeps the score down and gives your offense a chance and good field position. You need good kickers. Take out any one or two or three of those things and you can become very average. I think the main thing, though, is that you have 53 players and 13 coaches and a front office and other personnel and it all has to work together. It has to be one heartbeat. You gotta have that.''
The Colts' strength is their defense. This is a deep club. This is a Super Bowl contending club. Take solace, Jets. Look upward, Saints. Peek over here, Bucs. If the Colts can do it, any team can do it.
And the Colts are doing it.

subset: commoncrawl


text:
All Streets, as the name suggests, is a road map of all the streets in the 48 contiguous United States. The folks over at Fathom Information Design literally meant all the streets. There are 240 million individual road segments included in their map.
What's missing from the map are just about all other cartographic elements. There are no borders or rivers. However, quite a bit can still be inferred about the geography of the United States from this map. Areas sparsely scattered with roads indicate rural or mountainous regions, while a dense concentration can be associated with urban areas.
The All Streets map is data, cartography, and art in harmony. Poster prints of the map are available online. Fathom is donating half the proceeds to Kiva, the non-profit microfinance organization.
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'The Flash' Recap: "Flash of Two Worlds"
2 hours ago
On Earth Two, my doppelganger is a Pulitzer Prize-winning journalist.
On Earth Two, my doppelganger is a Pulitzer Prize-winning journalist.
Lincoln is on the run and the agents are in hot pursuit as Hunter and May infiltrate Grant's new entourage.
The graphic novel is being made into a movie in connection to a Night of The Living Dead universe
Also, Marvel might be bringing in some more female characters.
Welcome to Collider's Heroes, our weekly video round up of all the biggest news in the world of cinematic heroes and villains.
The star and executive producer talk about the upcoming 13 episode season, how it could be their last, what the return of Sarah Shahi's character, Sameen Shaw, means to the group, and more at NYCC.
The writer/director discusses the film's stunning set pieces, detailed costumes and turning traditional tropes on their head.
The two stars talk about the adult themes that are dealt with on the show, how much they used from the comics, memorable moments from filming, if 'Luke Cage' will also be an adult series, and more.
The 'Girls' creator will produce the comedy.
'Manhattan' returns to WGN America Tuesday, October 13th.


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Having earned the respect of her fellow fire fighters and the Phoenix community, Kara Kalkbrenner broke a few stereotypes and glass ceilings when she was named the city's first female fire chief in November 2014.
As part of its sesquicentennial celebrations, and for the first time in its history, Ottawa University hosted an Ottawa Powwow conducted by the Ottawa Tribe of Oklahoma on Saturday, April 18.
Fall 2015
Can you Feel It?
The energy? The excitement? If you've been on the Ottawa campus recently, there's no mistaking it. If you haven't, it's worth coming back!
Students were totally stoked last fall when they arrived on the campus to the new Gibson Student Center. Now, students, faculty, staff and the community are enjoying the beautiful and modern Gangwish Library. While pictures are great, not until you actually see it can you understand what all the noise is about. New conference spaces, huge atrium, beautiful fire and water feature, media lab, history mural, coffee shop and bookstore - not to mention the actual library with all its new technology and learning spaces. The Gangwish Library is stunning, hands down.
Incredibly, though, it's just one piece of excitement. when you add a huge freshman class, the new sport of lacrosse, new field turf, the start of the Fredrikson Chapel upgrades, the promise of more winning athletic seasons, and the upcoming 150th celebrations, it's easy to see why the College campus is abuzz. Ant there's more to come!
We invite you to catch the excitement by visiting campus this fall - especially during the Homecoming and Sesquicentennial Celebrations on October 15-18. You'll be glad you did!.
While students were transitioning from school day sot summer fun, Nori Hale '71 within the Advancement Office.
Hale was promoted to the position of Leadership Gift Officer, where she will widen the University's donor base and secure gifts for its annual fund, capital campaigns and various...
Under the direction of Stevens and women's head coach Brittany Frankenfeld both teams will compete for Kansas Collegiate Athletic Conference (KCAC) championships in their inaugural season after the conference added lacrosse to their list of championship sports in July.
The men's basketball team is bringing a familiar face back to Wilson Field House.
Phil McClintock '10 has been named Assistant Men's Basketball Coach, returning to the court where he was a two-time NAIA All-American and four-year letterman. In addition to working with the post players, he'll...
For the first time in program history, the Ottawa University men's basketball team will take on national powerhouse the University of Kentucky in an exhibition game on Nov. 2 at historical Rupp Arena in Lexington, Kent. Tip-off time will be announced at a later date.
In 1913, students from Ottawa University in collaboration with others from small colleges, founded Pi Kappa Delta, a forensics honorary society. The founding members used the first letters of the Greek phrase, Peitho Kale Dikaia, which means "the art of persuasion, beautiful and just."
Winter 2014
Fall 2013


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Four men charged with the fatal carjacking of a young lawyer at the Mall at Short Hills, just before Christmas all pleaded not guilty at their arraignment Wednesday in Newark.
Senate Vote Advances Jobless Benefits Bill
Legislation to renew jobless benefits for the long-term unemployed has cleared an initial Senate hurdle, but the bill's fate remains in doubt as President Obama offers his support for the extension.
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2014 Dawns on Legalized Pot in Colorado
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Concierge Care
Our patients are part of the PPMA family and our goal is to act as their advocates both in sickness and in health to ensure they receive what we believe is the best medical care possible. For a small monthly fee, you will have the dedicated services of PPMA, prompt attention and care, as well as a partner committed to your good health.
Q. What are the benefits of concierge medicine?
Unlike most doctors, we limit the size of our practice to only a few hundred so we can focus on your needs and provide you with unprecedented medical attention. We are well trained and skilled in disease detection and treatment, yet our primary focus is on optimal wellness and disease prevention. Under our care, patients often become so healthy; medications are no longer required for their daily routine.
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She was the only child of parents who were amazing entrepreneurs. Her mom owned a small health food store in Cape Cod, Massachusetts. Her dad, was founder of Cape Cod Potato Chips. Ever since her mom put the first carob covered rice cake in her lunch box as a kid, she has had a passion for making healthy food taste ...
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'The Woman In Black' Is the Most Successful British Horror Film in 20 Years
James Watkins-directed flick beats other Brit horror films with $23 million-plus U.K. box office take.
LONDON – Daniel Radcliffe's first post-Harry Potter role in British horror flick The Woman In Black has helped scare up record breaking figures for a British fright flick.
The Hammer-produced film, directed by James Watkins and adapted by Jane Goldman from the classic novel by Susan Hill, has spent three weeks at number one in the U.K. boxoffice ringing up a total of £14.6 million ($23.2 million) from 457 screens.
The makers claim those figures make it the U.K.'shighest grossing British horror film since records began 20 years ago.
The movie has now scared up more box office lucre than Hollywood -backed productions shot in the U.K. with British talent including The Others (£11.9 million, $18.9 million), Bram Stoker's Dracula (£11.5 million, $18.2 million) and Interview with a Vampire (£10.7 million, $17 million), as well as home-grown horrors such as Shaun Of The Dead (£6.7 million, $10.6 million) and 28 Days Later (£6.3 million, $10 million).
Hammer CEO and president Simon Oakes described the box office bonanza for the film as "an important achievement that affirms the re-birth of Hammer."
Hammer is developing a slate of genre films including The Quiet Ones and Gaslight, "both of which will shoot in the U.K.," Oakes said.
Xavier Marchand, president of worldwide distribution, Alliance Films and managing director Momentum Pictures, said with The Woman in Black occupying the number one spot in the U.K. for three weeks and its performance stateside that has seen it take more than $50 million, it indicates how British films "can resonate not only here in the U.K. but also abroad."
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These Hualpha-Syn(A53T) transgenic mice display an age-dependent phenotype including progressive motor deficits, intraneuronal inclusion bodies and neuronal loss. This line may be useful for studying Parkinson's disease and other synucleinopathies associated with motor neuron loss and ubiquitinated inclusions in the brain stem and the spinal cord, Lewy bodies, and synaptic plasticity.
Michael K. Lee, University of Minnesota
|Genetic Background
|Allele Type
|Transgenic (Humanized sequence, Inserted expressed sequence)
Starting at:
|$246.90 Domestic price for female
|$320.90 Domestic price for breeder pair
The donating investigator reports that homozygous mice are not viable. Mice hemizygous for the MoPrP-Hualpha-Syn(A53T) transgene are viable and fertile, but the donating investigator reports that female carriers do not breed well. These Hualpha-Syn(A53T) mice (also called G2-3(A53T), Hualpha-Syn(A53T), PrPsynA53T, or A53TαS Tg mice [line G2-3(A53T)]) express the familial Parkinson's disease-associated A53T missense mutant form of human alpha-synuclein (alpha-Syn or αS). Northern blot analysis shows high transgene expression in brain tissues is directed primarily to the hindbrain by the mouse prion protein promoter. Transgenic mice from line G2-3(A53T) express the A53T mutant alpha-Syn protein at approximately six times the level of endogenous mouse alpha-Syn. Hemizygous mice spontaneously develop adult-onset neurodegenerative disease between 9-16 months of age (mean age of onset is approximately 13 months, approximately 90% penetrant), with a progressive motoric dysfunction leading to death within 14-21 days of onset. Approximately 10% of the transgenic animals do not develop locomotor phenotype by 16-18 months of age. Affected mice exhibit neuronal abnormalities (in perikarya and neurites) including pathological accumulations of alpha-Syn and ubiquitin. Brain regions also show alpha-Syn-dependent neurodegeneration associated with increased/abnormal detergent-insoluble alpha-Syn and alpha-Syn aggregation. In neurons exhibiting alpha-Syn pathology, disease onset is coincident with induction of endoplasmic reticulum chaperones. Hemizygous mice also have adult onset hyperactivity associated with D1 receptor and dopamine transporter-mediated alterations in dopamine neurotransmission. These A53T mutant alpha-Syn expressing mice have significantly greater in vivo neurotoxicity when compared with A30P mutant or wildtype alpha-Syn expressing mice on the same genetic background.
This model is being used to screen for neuroprotective agents in the NINDS-funded CINAPS project.
A 436 bp human alpha-synuclein cDNA bearing a familial Parkinson's disease-linked A53T missense mutation was inserted downstream of a mouse prion protein promoter. This MoPrP-Hualpha-Syn(A53T) transgene was used for pronuclear injection into one-cell (C3H/HeJ x C57BL/6J) hybrid mouse embryos. The resulting Hualpha-Syn(A53T) transgenic mice (founder line G2-3) were subsequently backcrossed to C57BL/6J mice for at least 20 generations prior to arrival at The Jackson Laboratory. Upon arrival, transgenic mice were additionally bred to C57BL/6J mice to establish the colony.
|Expressed Gene
|SNCA, synuclein, alpha (non A4 component of amyloid precursor), human
|Site of Expression
|Allele Name
|transgene insertion 23, Michael K Lee
|Allele Type
|Transgenic (Humanized sequence, Inserted expressed sequence)
|Allele Synonym(s)
|A53T-syn; G2-3; Hualpha-Syn(A53T); MoPrP-Hualpha-Syn(A53T); PrPsynA53T; Tg(Prnp-SNCA*A53T)23Dpr
|Gene Symbol and Name
|Tg(Prnp-SNCA*A53T)23Mkle, transgene insertion 23, Michael K Lee
|Gene Synonym(s)
|A53T-syn; G2-3; Hualpha-Syn(A53T); MoPrP-Hualpha-Syn(A53T); PrPsynA53T; Tg(Prnp-SNCA*A53T)23Dpr; Tg(Prnp-SNCA*A53T)23Dpr; transgene insertion 23, Donald L Price
|Prnp, prion protein, mouse, laboratory
|Expressed Gene
|SNCA, synuclein, alpha (non A4 component of amyloid precursor), human
|Strain of Origin
|(C3H/HeJ x C57BL/6J)F1
|Mutations Made By
Michael Lee, University of Minnesota
When maintaining a live colony, hemizygous males are bred to wildtype female siblings or to inbred C57BL/6J females. The donating investigator reports that female carriers do not breed well and homozygotes are not viable.
The average number of mice provided from recovery of our cryopreserved strains is 10. The total number of animals provided,
their gender and genotype will vary. We will fulfill your order by providing at least two pair of mice, at least one animal of
each pair carrying the mutation of interest. Please inquire if larger numbers of animals with specific genotype and genders
are needed. Animals typically ship between 10 and 14 weeks from the date of your order. If a second cryorecovery is needed in
order to provide the minimum number of animals, animals will ship within 25 weeks.
The genotypes of animals provided may not reflect the mating scheme utilized by The Jackson Laboratory prior to cryopreservation, or that discussed in the strain description. Please inquire about possible genotypes which will be recovered for this specific strain. The Jackson Laboratory cannot guarantee the reproductive success of mice shipped to your facility. If the mice are lost after the first three days (post-arrival) or do not produce progeny at your facility, a new order and fee will be necessary.
Cryorecovery to establish a Dedicated Supply for greater quantities of mice. Mice recovered can be used to establish a dedicated colony to contractually supply you mice according to your requirements. Price by quotation.
Terms are granted by individual review and stated on the customer invoice(s) and account statement. These transactions are payable in U.S. currency within the granted terms. Payment for services, products, shipping containers, and shipping costs that are rendered are expected within the payment terms indicated on the invoice or stated by contract. Invoices and account balances in arrears of stated terms may result in The Jackson Laboratory pursuing collection activities including but not limited to outside agencies and court filings.
The Jackson Laboratory has rigorous genetic quality control and mutant gene genotyping programs to ensure the genetic background of JAX® Mice strains as well as the genotypes of strains with identified molecular mutations. JAX® Mice strains are only made available to researchers after meeting our standards. However, the phenotype of each strain may not be fully characterized and/or captured in the strain data sheets. Therefore, we cannot guarantee a strain's phenotype will meet all expectations. To ensure that JAX® Mice will meet the needs of individual research projects or when requesting a strain that is new to your research, we suggest ordering and performing tests on a small number of mice to determine suitability for your particular project.
"MICE" means mouse strains, their progeny derived by inbreeding or crossbreeding, unmodified derivatives from mouse strains or their progeny supplied by The Jackson Laboratory ("JACKSON"). "PRODUCT(S)" means biological materials supplied by JACKSON, and their derivatives. "SERVICES" means projects conducted by JACKSON for other parties that may include but are not limited to the use of MICE or PRODUCTS. "RECIPIENT" means each recipient of MICE, PRODUCTS, or SERVICES provided by JACKSON including each institution, its employees and other researchers under its control. MICE or PRODUCTS shall not be: (i) used for any purpose other than internal research, (ii) sold or otherwise provided to any third party for any use, or (iii) provided to any agent or other third party to provide breeding or other services. Acceptance of MICE, PRODUCTS or SERVICES from JACKSON shall be deemed as agreement by RECIPIENT to these conditions, and departure from these conditions requires JACKSON's prior written authorization.
In case of dissatisfaction for a valid reason and claimed in writing by a purchaser within ninety (90) days of receipt of, PRODUCTS or SERVICES, JACKSON will, at its option, provide credit or replacement for the PRODUCT received or the SERVICES provided; JACKSON makes no other representations and this shall be the exclusive remedy of the purchaser. Please note specific policy for live mice.
Consistent with the requirement for a written understanding regarding animal care and use, the JACKSON Animal Care and Use Committee will review the animal care and use protocol(s) associated with any SERVICES to be performed at JACKSON, and JACKSON shall have ultimate responsibility and authority for the care of animals while on site or in JACKSON custody.
In no event shall JACKSON, its trustees, directors, officers, employees, and affiliates be liable for any causes of action or damages, including any direct, indirect, special, or consequential damages, arising out of the provision of MICE, PRODUCTS, or SERVICES, including economic damage or injury to property and lost profits, and including any damage arising from acts or negligence on the part of JACKSON, its agents or employees. Unless prohibited by law, in purchasing or receiving MICE, PRODUCTS, or SERVICES from JACKSON, purchaser or recipient, or any party claiming by or through them, expressly releases and discharges JACKSON from all such causes of action or damages, and further agrees to defend and indemnify JACKSON from any costs or damages arising out of any third party claims.
MICE, PRODUCTS or SERVICES are to be used in a safe manner and in accordance with all applicable governmental rules and regulations.
The foregoing represents the General Terms and Conditions applicable to JACKSON's MICE, PRODUCTS or SERVICES. In addition, special terms and conditions of sale of certain MICE, PRODUCTS, or SERVICES may be set forth separately in JACKSON web pages, catalogs, price lists, contracts, and/or other documents, and these special terms and conditions shall also govern the sale of these MICE, PRODUCTS and SERVICES by JACKSON, and by its licensees and distributors.
Acceptance of delivery of MICE, PRODUCTS or SERVICES shall be deemed agreement to these terms and conditions. No purchase order or other document transmitted by purchaser or recipient that may modify the terms and conditions hereof, shall be in any way binding on JACKSON, and instead the terms and conditions set forth herein, including any special terms and conditions set forth separately, shall govern the sale of MICE, PRODUCTS or SERVICES by JACKSON.


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Hotel Highlights
- Right in the heart of historic Verona
- Brilliant food, courtesy of a Michelin-starred chef
- Frescoes, parquet floors and oil paintings
A mediaeval-modern mash-up dominates Palazzo Victoria hotel, where Roman ruins, Ionic pillars and 14th-century frescoes share space with white-leather sofas, projector screens and walls clad in concrete and acid-green lichen. There's a Michelin-starred chef in the kitchens, a pool table in the salon, and white-and-gold Jacuzzis in the shiny marble bathrooms.
Smith Extra
Here's what you get for booking Palazzo Victoria with us:
A can of Tuscan extra-virgin olive oil by Fattoria I Veroni
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Respond to Review of The Vegan Joint
by DawnOfTheRent
Review: I really enjoyed the food here, I'm not sure if they're like the vegan house by Hollywood but a lot of the menu looked similar. I ordered some sort of chicken plate, enjoyed all of it. (they also had this cake/pie "tiramisu": boy howdy it's good).
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text: Cheer on your team as they take it to the net for a win with the Tervis Oklahoma City Thunder 24 oz. Tumbler, which features a team logo to show your fan pride. Great for both hot and cold beverages, this tumbler is designed to reduce condensation and the included lid helps to prevent spills. Microwave, dishwasher and freezer safe. Fits most cup holders. Made in USA.


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With the help of RootsTech and FamilySearch.org, I had the honor and privilege to interview Chris van der Kuyl, CEO of the Scotland-based company brightsolid. Brightsolid is the parent company of the genealogy sites Find My Past UK, Find My Past IE, Find My Past AU, Genes Reunited, Scotland's People, The British Newspaper Archive, 1901 Census Online, and 1911 Census. Additionally, they're entering the American market right now with CensusRecords.com and later in the year with Find My Past US.
So, try out CensusRecords.com and see how you like it. And? Let them know how you like it. They really want to know what you think.
Mr. van der Kuyl was an incredible interviewee who made my job very easy, which I am extremely thankful for.
If unable to view the video here, below is the direct link to it on YouTube:
2/25/2012 08:36:08
You're getting good at this, Caroline. Excellent interview - good questions and follow-ups. The guy likes to talk, doesn't he?
Caroline Pointer
2/25/2012 11:40:56
Thanks Randy! Yes, he definitely likes to talk which made it SO much easier for me. LOL.
Leave a Reply.
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April 2015


text: My team rented Yew Tree Farmhouse for a 3 day retreat this June. The Farmhouse is situated in a quiet village near the Thames. The house and grounds are beautiful. The house was extremely clean and had everything needed to comfortably host a group of 14-17 people. We had our food delivered on the first day, had meetings in the... more


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♡ Hiihiii I'm Allie, & I'm vegan. Nice to meet you ~ ♡
The full modern day hippie basically!
I am awesome. That is all.
Free lance artist turned mum<3 I'm a single Pringle with a tiny herbivore<3 Together we help spread the vegan word.
Helping out in any way that I can. <3
I try to all I can do for animals. I'm 21 I've been vegan since December of 2007 and vegetarian 9months before that. I love reading animal rights books and thats about the only book you can get me to read. I have a wonderful son who I am bring up vegan!
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TEAC MP-600 Media Player is Thin and Crisp
We like our MP3 players small and wafer-thin, and this TEAC MP-600 qualifies as both because it's about the size of a Triscuit. Even though it's just 3 inches wide and 9mm thin, it still shows off a 3.5-inch touchscreen up front, and packs an FM tuner along with digital voice recording inside. Take the jump for a big… »


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June 20th, 2015 - January 3rd, 2016
Pacific Science Center Gets Gross (for science)Open Daily 10 a.m.-6 p.m
Why Do We Belch?
September 10th, 2015 - November 25th, 2015
Twilight Zone Art ShowTuesday through Saturday, 10 am to 4 pm.
You're About To Enter Another Dimension!
September 25th, 2015 - October 18th, 2015
The Rep Opens Season with Classic Play by Arthur MillerTuesday – Sunday 7:30 pm Saturday and Sunday Matinees 2:00 pm
An American Classic
September 29th, 2015 - October 31st, 2015
15 Acre Corn Maze9:30 a.m. - Dusk
October 2nd, 2015 - October 17th, 2015
Willkommen to Oktoberfest!October 2-3, 9-10, 16-17
Experience Oktoberfest in the Bavarian town of Leavenworth
October 9th, 2015 - October 11th, 2015
Crab Fest 2015!Friday October 9: 12:00noon – 10:00pm Saturday October 10: 9:00am – 10:00pm Sunday October 11: 9:00am – 5:00pm
Come for the Food, Stay for the Fun!
Sunday, October 11th, 2015
Two Shows from One of a Kind Comic Kathy Griffin7:00pm & 9:00pm
Kathy G Skewers Pop Culture
October 23rd, 2015 - October 25th, 2015
6th Annual Ghost ConferenceFriday: 4-11:30pm, Saturday: 8:30am -10:45pm, Sunday: 9:30am – 4:00pm
Want to do a little ghost hunting this Halloween?
October 23rd, 2015 - October 24th, 2015
Bonfires, Beaver Pelts and Bogeymen7:00 pm - 9:00 pm
Living History in Point Defiance Park October 23 -24 Fort Nisqually, Tacoma The whole family will enjoy listening to costumed storytellers spin spooky tales around roaring bonfires on a crispy autumn night. Enjoy a cup of warm cider and stories of the …
October 24th, 2015 - October 25th, 2015
Pumpkin Bash & Trick or Treating9:30am – 3:00pm
Halloween at the zoo October 24 – 25 Woodland Park Zoo Enjoy treats and watch animals play with pumpkins at Pumpkin Bash at Woodland Park Zoo. Pumpkins for the animals, trick-or-treating, and more are in store for little goblins and ghosts at Woodland …
Saturday, October 24th, 2015
Comedian Tim Allen8:30pm
Tim Allen Live October 24 Emerald Queen Casino He's been Tim the Tool Man, the voice of Buzz Lightyear, starred in The Santa Clause, and Wild Hogs, and of course has slayed live audiences with his "cut to the truth" humor about the differences between …
Wednesday, October 28th, 2015
Power of a Promise
Join us in support of Breast Cancer Awareness and Susan G. Komen Puget Sound
October 30th, 2015 - November 1st, 2015
Riverdance 20Friday: 8pm, Saturday: 2pm & 8pm, Sunday: 1pm & 6:30pm
20th Anniversary Tour of Riverdance! October 30 – November 1 Paramount Theatre Ready for a little rhythm? The international Irish dance phenomenon is back in Seattle by popular demand in RIVERDANCE- THE 20TH ANNIVERSARY TOUR for three days only. …
November 5th, 2015 - January 31st, 2016
My Fair Lady at the Village TheatreWednesdays and Thursdays at 7:30 PM Fridays at 8:00 PM Saturdays at 8:00 PM Sundays at 7:00 PM plus Matinees
The Rain In Spain Falls Mainly on The Plain
Saturday, November 7th, 2015
Veterans Day Parade11:00 am
Thank Them For Their Service
Saturday, November 7th, 2015
Hal Holbrook is Mark Twain7:30pm
A Living, Breathing, American Masterpiece!
November 20th, 2015 - December 19th, 2015
The Twilight Zone Live!Thursdays, Fridays and Saturdays at 8:00 pm
You've Just Crossed Over…
Monday, November 30th, 2015
Providence O'Christmas Trees
@ The Westin Seattle November 30th | 11am – 1pm Join us in Seattle at The Westin Hotel for the Providence O'Christmas Trees Silver Bells Luncheon from 11am-1pm! Come and see designer decorated Christmas Trees and enjoy a holiday themed fashion show fea …
December 11th, 2015 - December 13th, 2015
Disney's Beauty and The Beast Returns to SeattleFriday 8pm, Saturday 2pm & 8pm, Sunday 1pm & 6:30pm
It's a "Tale As Old As Time"
Saturday, December 12th, 2015
Kirkland Performance Center presents… KPC's Campy Christmas8:00PM
Holiday traditions turned on their heads


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(Updated 12:02 p.m.) Former R.I House Speaker Gordon D. Fox has been charged with three felonies – bribery, wire fraud and filing a false tax return – and pending court approval of a plea agreement, he could spend up to three years in prison, according to U.S. Attorney Peter F. Neronha. more
By Eli Sherman
(Updated, 12:55 p.m.) Santander Bank has reached an agreement to change the way it screens applicants of checking and savings accounts amid concerns the current practice is slanted against low-income consumers. more
By Eli Sherman
PROVIDENCE – The R.I. Department of Labor and Training has referred a complaint involving possible employee criminal conduct to the state police for investigation. more
By Lori Stabile
A federal court jury on Thursday convicted a 34-year-old Providence man in connection with a counterfeit check scheme that defrauded local banks of at least $800,000. more
By PBN Staff
(Updated 3:54 p.m.) State Rep. Joseph S. Almeida, 57, of Providence was arrested by State Police Tuesday on a felony charge relating to unlawful appropriation of campaign contributions, the state agency announced. more
By Mary MacDonald
Rhode Island defeated a legal challenge from OppenheimerFunds Inc. to its plan to sell $594 million of debt backed by payments from tobacco companies that agreed in 1998 to reimburse states for expenses associated with smoking-related illnesses. more
By Chris Dolmetsch
An HIV-positive man has filed a lawsuit in federal court because he said HealthSource RI forces him to pay for women's abortions as a condition to obtain health insurance through the state exchange. more
By PBN Staff
A group of Rhode Island motor vehicle companies has filed a court complaint seeking to block a state requirement that all vehicles for hire charge customers at least $40 a trip. more
By Mary MacDonald
Preliminary data indicates both property and violent crimes dropped in 2014 in Providence, according to a news release. Reported incidents dropped 15 percent, according to Mayor Angel Taveras, Commissioner Steven M. Pare and Police Chief Col. Hugh T. Clements Jr. more
By Mary MacDonald
Attorney General Peter F. Kilmartin said that the state of Rhode Island will receive $198,000 through a settlement with T-Mobile over "mobile cramming," in which charges for third-party services on consumers' mobile telephone bills that had not been authorized by the consumer are included. more
By PBN Staff


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Acilarin çocugu Küçük Emrah Koca Kafalar
by Metacafe Affiliate U (10/02/07) 5,878 views
acilarin çocugu küçük emrah koca kafalar
acilarin çocugu küçük emrah koca kafalar
No description
one of the most known titles of "little" Emrah (at present he sings...
(2x) ben bir yaprak daliyim suyum sensin yarim damlada bana canlan...
No description
The woman version of "Little" Emrah, very nice and intensive voice....
telefonu acmaan abi
sanatci 2.bergen
sanatci 2.bergen
sanatci 2.bergen
Koca Kafalar Kucuk Emrah Acilarin Cocugu Ozgun Namal Kanal D 11`inc...
KK 11`cu bolum
ve o an
bergen 2
There's a new meme -- and these guys are going to stop it. Maybe.
Food that's so comfortable, it'll make you pass out.
In honor of Halloween, which apparently occurs at some point in the near future, we thought we'd go back to one of the classic horror films, A Nightmare on Elm Street.
Ownage Pranks put up a Craigslist ad offering free voice acting lessons. An enthusiastic man responded back right away, and the things they got him to repeat were absolutely hilarious.
We're going the Hollywood route and releasing this video in two parts. You can give us a sin for that, but frankly, we had no choice.


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Associate Professor of Architecture Bruce Swetnam and the Comprehensive Studio Featured on WRFL
Sep 22, 2015
Associate Professor of Architecture Jason Scroggin's work featured in UK's Experience Art With SAB
Sep 21, 2015
Architect Magazine features work by UK/CoD School of Architecture Professors Filson and Rohrbacher
Sep 1, 2015
The Lexington Herald-Leader's Tom Eblen recently wrote a recap of UK/CoD's Mayor Institute on City Design. The event, held in conjunction with the National Endowment of the Arts, occured on October 10-12 in Lexington.
Seven mayors from cities across the eastern United States met with a team of design professionals to discuss design issues facing their cities.
"It was a terrific, creative, collaborative learning process," said Liz Rogan, president of Lower Merion's Board of Commissioners. "I learned that there are creative solutions and processes and different ways of seeing things."


text: There is a big room at the back, which is perfect for a birthday group. They have the most fun version of the "Happy Birthday" song that they will play over the speakers. The staff are friendly and nice. Most importantly the food is good and the portions are generous. The mousakka, chicken... read more


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Used Cadillac XTS in Portland, OR
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Why buy used?831 new Cadillac XTS listings
|by Sparky222
"Expensive for what is delivered, fast depreciation."
Very similar to other GM products, especially Malibu and Impala. Most distinctive feature is front led running lights. Handling a bit numb, but secure, even with magnetic ride control .Braking with Brembo fronts is excellent, acceleration not so much, but this is not a V-8, and louder than expected under full bore. Transmission shifts are silky smo...
2014 Cadillac XTS Luxury AWD Used Cars in Vancouver, WA 98661
Price: $31,977
Mileage: 15,924
Location: Vancouver, WA 98661
2013 Cadillac XTS Premium AWD Used Cars in Hillsboro, OR 97123
Price: $35,995
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2014 Cadillac XTS Luxury AWD Used Cars in Portland, OR 97232
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All hotels in Austin, Texas, United States of America
1 room, 2 adults
15707 Oak Grove Blvd, Austin, TX, 78734, United States of America, 866-299-2910
Holiday Inn Express Hotel & Suites Lakeway, Austin
Lowest price$189 CAD
available on 10/11/2015
Best Price Guarantee
Suburban hotel in Austin with outdoor pool, health club
- Free buffet breakfast, free WiFi, and free parking
Main amenities
- 93 smoke-free guestrooms
- Outdoor pool
- Health club
- Business center
- Air conditioning
- Daily housekeeping
- Computer station
- Front desk safe
- Laundry service
- Meeting room
- Tour/ticket assistance
What's around
- Falconhead Golf Club (5.4 mi / 8.7 km)
- Tom Hughes Park (6.5 mi / 10.5 km)
- Windy Point Park (8.8 mi / 14.2 km)
- One World Theatre (12.5 mi / 20.2 km)
- Bull Creek District Park (12.9 mi / 20.8 km)
When visiting Austin, Holiday Inn Express Hotel & Suites Lakeway is a great choice to consider. The outdoor pool is a great spot for a morning swim, and guests can get a workout in at the health club. Flat-screen TVs, free WiFi, and free wired Internet are offered in all rooms.
It was pleasant for the two nights we were there there. The hotel was booked due to a…
My wife and I enjoyed our stay,albeit for only one night. My only complaint is that…
TripAdvisor Traveller Rating: 4
From 106 reviews
Enter dates to check prices
Hotel Info: 866-266-9798from $189 CAD
- One King Bed, Non-Smoking, Accessible
- Room, 1 King Bed, Accessible, Non Smoking
- One King Bed, Non-Smoking, Roll In Shower
- One King Bed Non-Smoking Accessible
- Suite, 2 Queen Beds, Non Smoking
- Two Beds, Non-Smoking, Accessible
- Suite, Accessible, Non Smoking
- Suite, Non-Smoking, Accessible
- Room, 2 Queen Beds, Non Smoking
- Suite One King and One Queen Bed
- Room, 1 King Bed, Accessible, Non Smoking
- Standard Room
- Suite One King Bed Non-Smoking
- Room, 1 King Bed, Non Smoking
Also popular with travellers from Canada
At a glance
Key facts
Hotel size
- This hotel has 93 rooms
- This hotel is arranged over 3 floors
- Check-in time starts at 3 PM
- Check-out time is 11 AM
Required at check in
- Credit card or cash deposit required
- Government-issued photo ID required
- Minimum check-in age is 21
Travelling with others
- Pets not allowed (service dogs only)
Extra people
- Roll-away beds available, charges may apply*
- Free WiFi and wired Internet access in public areas
- Free WiFi and wired Internet access in rooms
- Free self parking
Other information
- Smoke-free property
Nearby Two Star Austin hotels
In the hotel
Food and drink
- Free buffet breakfast
Things to do
- Outdoor pool
- Health club
- Golfing nearby
- Hiking/biking trails nearby
Working away
- Business center
- Conference space size (feet) - 750
- Conference space size (meters) - 70
- One meeting room
- Computer station
- Tours/ticket assistance
- Dry cleaning/laundry service
- Laundry facilities
- Safe-deposit box at front desk
In the room
Home comforts
- Air conditioning
- Coffee/tea maker
- Iron/ironing board
Freshen up
- Hair dryer
Be entertained
- 40-inch flat-screen TV
- Pay movies
- Premium TV channels
Stay connected
- Desk
- Free WiFi
- Free local calls
- Daily housekeeping
Top Austin hotels
Recently reviewed hotels in Austin
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"Clean, really good breakfast, stay away from the train side"Aug 29, 2015
- Homewood Suites by Hilton Austin SouthFrom
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"Great location near park and river and bat bridge. Easy walk to town."Aug 26, 2015
- Best Western Plus Austin City HotelFrom
"Best value"Aug 23, 2015
Nearby hotels
Holiday Inn Express Hotel & Suites Lakeway, Austin's small print
Mandatory fees
Cash deposit: USD 100 per stay
Optional extras
Rollaway beds are available for USD 15 per night
We have included all charges provided to us by this hotel. However, charges can vary, for example, based on length of stay or the room you book.


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Fort Davis
West Texas Wildfires Burn Again
One year after the Rock House fire, more than 20,000 acres in Jeff Davis County are aflame.
One year after the Rock House fire, more than 20,000 acres in Jeff Davis County are aflame.
Victorian splendor in Jefferson, manly minimalism in Archer City: Check into ten charter members of Texas' inn crowd.
Big, breezy porches in Port Aransas, the only heated pool for miles in Marathon: You'll get more than just a bed and breakfast at these ten appealing places to stay.
Choose Your Own Texas Adventure
When it comes to traveling around the state, it's easy to be overwhelmed by the sheer number of destinations to choose from. One of the things I love most about Texas is that you can drive a few hours (or more than a few hours) in one direction and be at, say, the beach […]
All the Pretty Horses
The scenes in Wayne Baize's drawings and paintings are rendered with so much self-restraint they're nearly austere. A look at one of America's most distinctive Western artists.
Wayne Baize, one of America's most admired cowboy artists, lives amid the soaring mountains and wide-open plains. But his eye is drawn to something else entirely.
Fort Davis
The "most important town in the Trans-Pecos" has traded in its military bearing for a healthy dose of tourist appeal.
Fort Davis.
Trial by Fire
This year will be remembered for a terrifying series of wildfires that ravaged almost every corner of the state, from Bastrop County to Possum Kingdom, from Amarillo to the Mexican border. All told, the flames torched nearly three million acres in an unprecedented disaster—and perhaps an omen of things to come. What did it look and feel like on the ground the year Texas burned?
It will be remembered as the year of smoke and devastation, as drought-fueled flames wreaked unprecedented havoc across Texas, from Bastrop County to Possum Kingdom. A photographic and oral history of the 2011 wildfires.
The Weird, Weird West
Location: Fort Davis What You'll Need: Cowboy hat, canteen The pleasures of Fort Davis aren't as arty or oddball as the ones in nearby Marfa or Alpine, but that's not to say that things aren't strange. For example, which is weirder: that Fort Davis and Jeff Davis County, in the mountains of far West Texas […]
How to Customize a Cowboy Hat
The Material A cowboy hat is a beloved possession: It fans fires, it blocks the rain, it gives shade—and it lends authenticity at any honky-tonk or greased-pig contest. But it's also an extension of one's personality, so commissioning one takes serious thought (and serious dough: from $300 to $1,500). The first decision? Felt or straw. […]
The Final Frontier
To solve the mystery of dark energy—a phenomenon that could reveal the origins of the universe—Texas astronomers need a $34 million telescope and a little bit of luck. There are only two problems: it may not be dark, and it may not be energy. In fact, it may not exist at all.
Karl Gebhardt and Gary Hill, two astronomers from the University of Texas at Austin, are racing to solve one of the greatest mysteries in science: What is dark energy? How does it work? Can it explain the origins of the universe? There's only one problem. Dark energy may not actually exist.
Out There
What I saw at the Republic of Texas standoff.
For seven days Rick McLaren and his armed cohorts were holed up in their Republic of Texas "embassy" while reporters dug for stories, lawmen kept watch, and the residents of nearby Fort Davis wished they'd all go away.


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San Clemente-based hotel investment company Sunstone may surrender 11 distressed properties to its lender, according to a third-quarter financial report. If it does, it could bring its total number of forfeited hotels to 14.
Sunstone recently dumped San Diego's W Hotel and the Marriott Ontario Airport Hotel because its lenders wouldn't renegotiate loans. Now it wants Massachusetts Mutual Life Insurance Co., the administrative agent for a $246 million mortgage secured by 11 hotels – including several in Southern California – to rework that loan.
If Mass Mutual doesn't do it, Sunstone may forfeit the hotels, which comprise 2,587 rooms and are valued at $288.8 million, Sunstone said. The hotels could generate cash-flow loss of $18 million this year, Sunstone officials said in a transcript of an earnings call released late last week.
The company said revenue per available room is down more than 20 percent at its Orange County and Los Angeles hotels.
"Giving back the keys in general leads to a broader concern that it might alienate you from lenders in the future, but only time will tell," said Smedes Rose, an analyst at New York investment bank Keefe, Bruyette & Woods.
Rose said he thinks Sunstone will likely forfeit the hotels because company leaders say they are old assets that don't fit in with the real estate investment trust's other properties.
Mass Mutual would not comment on the ongoing negotiations, said Marc Cybulski, a spokesman for the insurer.
Sunstone, which has about 40 hotel holdings nationwide, including the Fairmont Newport Beach, recently defaulted on its mortgage for the Renaissance Westchester in White Plains, N.Y., and is trying to modify that loan, according to the earnings report. It is currently finishing loan amendments to its Renaissance Baltimore property.
All this comes during a time of tight credit in the commercial mortgage market.
According to Sunstone's third-quarter report, it skipped a Nov. 1 payment on its Mass Mutual loan, which matures in 2011, to push the lender to extend the due date or decrease the principal balance. Sunstone officials said the current value of the 11 hotels is less than the outstanding principal.
Even as the company has whipped out its negotiating guns, its leaders also say they're looking into buying new properties. The company is looking for properties in high-performing markets, and officials noted that Boston and New York have been its best performers recently.
"In the last two weeks, we've delivered four expressions of interest for hotel acquisitions," Sunstone President Art Buser said in the transcript of the earnings call. "I'm spending about a third of my time now on acquisitions."
Sunstone doesn't have to worry about scaring away lenders for those acquisitions because it plans to use cash on hand, Rose said. Much of that cash was generated from a recent $158.6 million stock offering.
But Rose said the future of real estate investment trusts looks dim.
"The lodging REITs have been really hurt in general when it comes to earnings, and we think they will be like that for the next several quarters," he said.


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Os melhores sucos de Poços!
Foursquare © 2015 Lovingly made in NYC & SF
Hotspots WIFI Poços de Caldas
Created by Alvaro Guatura121 items • 39 followers
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Download Foursquare for your smart phone and start exploring the world around you!


text:
History lesson: What Ronald Reagan saidBreaking News
Mitt Romney: "It's disgraceful that the Obama Administration's first response was not to condemn attacks on our diplomatic missions, but to sympathize with those who waged the attacks."
..[B]ecause Republicans have frequently likened President Obama to Jimmy Carter, we were curious to learn how candidate Ronald Reagan responded to the worst foreign policy disaster on Carter's watch — the failed mission to rescue U.S. diplomats in Iran, resulting in the deaths of eight servicemen.
In April 1980, Reagan was still battling George H.W. Bush for the GOP nomination, while Sen. Edward M. Kennedy was challenging Carter for the Democratic nomination. This is excerpted from The Washington Post reporting on the political fallout:
Carter's presidential rivals were charitable. Republican George Bush supported the president's actions without reservation. Ronald Reagan and Edward Kennedy offered sympathy to the families of the dead troopers and called for national "unity."...
comments powered by Disqus
- Children should be taught about suffering under the British Empire, Jeremy Corbyn says
- Collateral damage: A brief history of U.S. mistakes at war
- East Germany's secrets are slowly being revealed
- William Buckley's FBI files released
- Graphic of the Week: Browse An Archive of 170,000 Depression-Era Photos
- Daniel Pipes says we should be worried that immigrants don't share western values
- Nobel Prize in Literature Awarded to journalist Svetlana Alexievich
- Niall Ferguson leaving Harvard for Stanford
- Integration Of Cheerleaders Was Difficult To Achieve
- New-York Historical Society to Open Women's History Center


text: We booked 3 nights in advance, but really we should have only booked one night and got the hell out of there. The shower so small and filthy we didn't take a shower for 3 days till we moved to our next hotel! There was dust everywhere. Dust and grime all over the heater. The place has character and a... More


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Book your room at Silver Beach Hotel
Please enter your travel dates.
Get an overview of this hotel3.5-star hotel near the beach
This family-friendly Saint Joseph hotel is located near the beach, within 2 mi (3 km) of Silver Beach, Harbor Shores Golf Club, and Lakeland Regional Medical Center. Lake Michigan College and Contessa Wine Cellars are also within 12 mi (20 km).
Hotel Features
Along with an indoor pool, this smoke-free hotel has a fitness center and a bar/lounge. Free buffet breakfast, free WiFi in public areas, and free self parking are also provided. Additionally, coffee/tea in the lobby, a business center, and meeting rooms are onsite.
Room Amenities
Rooms offer comforts like pillowtop beds and premium bedding, plus free WiFi and flat-screen TVs with satellite channels. Other amenities available to guests include balconies, refrigerators, and microwaves.Information missing or incorrect? Tell us!
Hotel Amenities
Hotel Amenities
Silver Beach Hotel features an indoor pool and a fitness center. A bar/lounge is on site where guests can unwind with a drink. A complimentary breakfast is offered each morning. A computer station is located on site and high-speed wired and wireless Internet access is complimentary.
This 3.5-star property offers access to a business center and meeting rooms. 2 meeting rooms are available. This beach hotel also offers coffee/tea in the lobby, laundry facilities, and dry cleaning/laundry services. Onsite self parking is complimentary.
Silver Beach Hotel is a smoke-free property.
- 24-hour front desk
- Bar/lounge
- Business center
- Coffee/tea in lobby
- Computer station
- Dry cleaning/laundry service
- Elevator/lift
- Fitness facilities
- Free breakfast
- Free self parking
- Free WiFi
- Free wired high-speed Internet
- Indoor pool
- Laundry facilities
- Meeting rooms
- Number of meeting rooms - 2
- Smoke-free property
Family Friendly Amenities
- Cribs/infant beds (complimentary)
- Free Wi-Fi
- Indoor pool
- Laundry facilities
- Microwave
- Refrigerator
Room Amenities
- Balcony
- Climate control
- Coffee/tea maker
- Free cribs/infant beds
- Daily housekeeping
- Desk
- Flat-panel TV
- Free WiFi
- Hair dryer
- In-room safe
- Iron/ironing board
- Microwave
- Phone
- Pillowtop mattress
- Premium bedding
- Premium TV channels
- Private bathroom
- Refrigerator
- Satellite TV service
Where to Eat
A complimentary buffet breakfast is served each morning between 7 AM and 9 AM.
Nearby Things to Do
Recreational amenities at the hotel include an indoor pool and a fitness center.
To make requests for specific accessibility needs, complete the "Special or Accessibility Requests" section of "Trip Preferences" when you book your hotel.
Hotel Policies
Check-in time starts at 3:00 PM
Minimum check-in age is 21
Check-out time is 11:00 AM
Payment types
Children and extra beds
- Children are welcome.
- Rollaway/extra beds are available for USD 20 per stay.
- Free cribs (infant beds)!
Pets allowed
You need to know
Extra-person charges may apply and vary depending on hotel policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
Optional extras
- Pet fee: USD 45 per pet, per stay
- A late check-out fee will be charged
- Rollaway bed fee: USD 20 per stay
We should mention
The property allows pets in specific rooms only and has other pet restrictions (surcharges apply and can be found in the Fees section). Guests can arrange to bring pets by contacting the property directly, using the contact information on the booking confirmation.


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Owning a firearm means nothing if you can't access it quickly. Here are 10 great fast-access gun safes for home defense.
Home Defense
Home Defense: AR vs. Shotgun
Which is the better choice for home defense?
What to Look for in a Home-Defense Handgun
Not all handguns are right for home defense. In fact, a nightstand gun is going to have a number of features that might make it unwieldy for many other forms of self-defense, such as concealed carry.
Video: Defensive Shotgun Patterning
Patterning a shotgun is typically thought of as a hunting exercise. But in this video, famed instructor Clint Smith demonstrates the importance of the practice for a defense smoothbore.
Armed Citizens in Action, August 2014
True stories of everyday armed citizens taking action to defend themselves and others.
Market Trends: Bullpup Shotguns and Moving Targets Big in Arizona
For a retailer in southern Arizona, it isn't handguns or ARs that have gotten the nod for home defense guns. Instead, bullpup shotguns have become the popular choice among the store's defense-minded patrons.
Combat Focus Shooting: An Experience in Effective Handgun Training
We didn't know what (or if) we would need to shoot, when, or from what position or distance. Sounds a bit like how a defensive shooting situation might happen, right?
Lawful Use of Lethal Force: Surviving Massad Ayoob's MAG20 Class
Do you know when you can lawfully use lethal force? Can you explain your self defense choices? If you have any doubt, Massad Ayoob's class can clear it up. I recently spent two very long and intense days in his MAG-20 Armed Citizens' Rules of Engagement course. Mr. Ayoob lived up to his reputation,...
Choosing a Handgun: Hand Size & Strength Matters
Whether you're interested in hunting, competition, concealed carry or self defense, you can find a handgun that fits. When deciding between a revolver and an autoloader, the size and strength of your hands is an important consideration.


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HOME > Britney And Kevin: Chaotic
Britney And Kevin: Chaotic
Kevin Federline asks court for sole custody of Britney Spears' children
Rapper Kevin Federline has promptly responded to Britney Spears' petition for divorce by filing for solo custody of their children in Los Angeles. Full Story
Report: Kevin Federline scraps reality show plans due to Britney chaos
Britney Spears and Kevin Federline previously had no problem letting fans in on their lifestyle, but the current situation surrounding the pop princess is apparently just too chaotic for Federline to let reality cameras role again.
Britney Spears divorcing former 'Chaotic' co-star Kevin Federline
Pop star and new mom Britney Spears filed divorce papers in Los Angeles Tuesday citing "irreconcilable differences" with husband and former "Britney and Kevin: Chaotic" co-star Kevin Federline.
'Chaotic' stars Britney Spears, Kevin Federline name new son 'Sutton'
Glamour couple Britney Spears and Kevin Federline have given their newborn son the decidedly patrician name, Sutton Pierce Federline.
Kevin Federline says he's going broke despite 'Chaotic' show payday
Rapper and Britney Spears husband Kevin Federline is going broke even though he made about $2 million off of the UPN show "Chaotic."
Britney Spears denies reports of second 'Chaotic' reality show season
Britney Spears says there's no truth to recent reports that she is considering a second season of Britney and Kevin: Chaotic, the failed 2005 UPN reality series that chronicled her pre-marriage relationship with husband Kevin Federline.
Former 'Chaotic' star Kevin Federline campaigns for pennies
Kevin Federline, Britney Spears' husband, joined Americans for Common Cents and Virgin Mobile in New York to push pennies.
Ex-'Chaotic' stars Britney Spears, Kevin Federline back in same bed
Britney Spears and husband Kevin Federline were said to be romantically back together when they were spotted on a short Miami Beach vacation.
Former 'Chaotic' star Kevin Federline becomes face of fashion line
A California clothing company has signed Britney Spears' husband, Kevin Federline, to feature him as part of its newest clothing line.
Former 'Chaotic' star Britney Spears says she has no regrets
Matt Lauer, New York host of "Dateline NBC," questioned Britney Spears about marriage, motherhood, housework and fame -- she has no regrets about any of it.
Former 'Chaotic' star Britney Spears to tell all in television interview
Britney Spears defends her parenting skills in an interview with NBC's Matt Lauer set to air Thursday, the network says.
Former 'Chaotic' star Britney Spears confirms she's pregnant again
U.S. pop star Britney Spears Tuesday confirmed during a surprise appearance on "Late Night with David Letterman" that she's pregnant again.
Report: Former 'Chaotic' star Britney Spears pregnant again
Pop star Britney Spears is pregnant with her second child by husband Kevin Federline, US Weekly reported Tuesday.
Britney Spears hubby Kevin Federline defends himself to Newsweek
Wannabe rapper Kevin Federline defends his reputation in this week's Newsweek, saying if reports were true, he would not be married to Britney Spears.
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