text:
Updated: New road.cc jersey design - have your say!
Tell us what you think, don't be shy...now we've added versions with your changes on too
We've always said we wanted road.cc to be more like an online club than anything else. That means everyone getting involved, and the latest way to do that is to spout forth on the goodness or otherwise of our mockup of the new road.cc jersey. You have spoken and we have listened… sort of. So now we've added some new versions inspired by your suggestions. Vecchiojo's white piping gets an airing as does his favourite pantone pink and in a fashion mash-up fringe's retro look meets PK Ripper's white idea. We'll probably do some more later too, but for now tell us what you think of this lot.
Eagle-eyed members will recognise our crrent favourite as a development of one of the potential Planet X-road.cc jerseys that we stuck on the site not so long ago. It didn't make it onto the back of the team, but it did get a lot of love, so we're thinking of making some for the season.Click on it for a closer look: there's still a bit of our favourite Argyle in there, of course.
Obviously you can't please all of the people all of the time but we'd like some feedback, so don't be backward in coming forward! And if you've got other designs that you think would work for a road.cc jersey, add them to you comments... ones you've found, ones you've loved or – even better – ones you've scrawled on the back of a fag packet and scanned in.
Your mum always told you that if you haven't got anything nice to say, it's better not to say anything. But hey, we're pretty tough skinned. So fire away*
*You don't have to say nasty things though. You can be nice too.
If you had launched it a few months ago maybe we could have signed Wiggins as he seemed to take so long to decide whether to wear a black/blue jersey or an Argyle one for 2010 - Roadcc would have given him the best of both worlds
I like it by the way
Twitter - @StuKerton
posted by stuke [356 posts]
29th January 2010 - 10:15
I'd wear it. Although I prefer white to black, killer to wash out though.
Does lack that little spark that would compliment the Road.cc quality
Otherwise...good job.
posted by AndyyK [40 posts]
29th January 2010 - 10:15
That's based on the one of the designs I preferred
I'd definitely wear that if you can make it big enough!
So, next questions.
How soon?
How much?
Done Nightrider 2013, 2014 and 2015 for Parkinson's UK. Next year Ride London
posted by jova54 [663 posts]
29th January 2010 - 10:50
me .... i want one now, right now .... excellent
when do we get them ????
still on the 3rd switch-back of Bwlch !
posted by therevokid [922 posts]
29th January 2010 - 11:24
Following Jo's suggestions, I would agree with his points.
The addition of another colour would also be really good - ideally one of the diamonds on front and back being pink. Off centre, of course.
posted by ourmaninthenorth [93 posts]
29th January 2010 - 11:42
Like the design, doesn't need any more embellishment. personally I'de prefer a magila rosa pink to the blue and please please make it out of sportwool.
Carpe Diem ab absentis: seize the day off
posted by Coodsta [110 posts]
29th January 2010 - 11:44
Argyle is not my favourite but I very much agree with the "meh" comment above.
I needs some extra colour(s) - at present it looks a bit flat and, well, boring.
posted by chiefoldmist [67 posts]
29th January 2010 - 12:58
mmm...well i mulled this one over whilst i ate my sardines and greens and i prefer a more simplistic and less jazzy number myself, (i expect thats why Rapha seem to do so well). Anyhow keep the colour down to black plus one other (blue, red, green etc) and keep the diamond Argle strip but make it more 'classic' looking perhaps..like this!
posted by Fringe [1097 posts]
29th January 2010 - 14:43
what's with the argyle still? That's so rockshox 2003!
Personally I wouldn't wear one as despite it being in the same colours as my new bike the colour proportions are in my opinion a bit wrong.
Am very much a fan of understated styling and this one I think would look better with the bulk colour being white as opposed to blue, with the diamonds in blue and red - like the old england football kit. At the moment it all looks a bit "altura commuter" - not a good look!
posted by the-yorkshire-p... [180 posts]
29th January 2010 - 14:53
Yorkshire, it just shows some continuity with the original road.cc jersey. I think that the arglye works.
not all carbon is the same.
posted by Jon Burrage [1081 posts]
29th January 2010 - 15:51
I like Fringe's option, the Rapha-esque style appeals...
Complicating matters since 1965
posted by DaSy [693 posts]
29th January 2010 - 16:20
posted by miuzikboy [60 posts]
29th January 2010 - 17:16
Perhaps the wording pedal powered on the cuff of each sleeve.
posted by Lantern rouge [20 posts]
29th January 2010 - 18:45
Fringe's suggestion in merino would definitely be something I'd buy (not convinced by sportwool though).
I like the main version and would buy it though I don't think its worthy of a merino build.
"too jazzy" - love it!
posted by TheHatter [811 posts]
29th January 2010 - 20:00
4 for me... the pink ones are a bit too "Rapha Condor" for me.
posted by jimmythecuckoo [1348 posts]
29th January 2010 - 20:01
I think if it was going to be pink we would need to go more pink. We are going to do something in merino too, but it's more likely to be long sleeved full zip and something you could wear on or off the bike.
posted by Tony Farrelly [4201 posts]
29th January 2010 - 21:11
kit 5, much as i like it, is too close to the RaphaCondorSharp team kit. not keen on the white on 7, would prefer a band of colour/black all the way round, but out of that new lot i really like 6.
i wonder if FUJI SERVETTO had these problems.."like the light brown skin poo-ish colour"
posted by Fringe [1097 posts]
29th January 2010 - 21:43
posted by sponging-machine [109 posts]
29th January 2010 - 21:45
How about a charity band on the arm like radioshack my vote would be for Cancer Research UK.
posted by Chris Deacon [154 posts]
29th January 2010 - 21:58
I was thinking along the same lines as Chris Deacon, £1? to charity for each purchase say.
I would like to see a little pink on it in the argyll pattern perhaps. Quite like the Giro etc but a full on pink is too much. Ibex did a nice jersey with a narrow pink band but for the ladies only
White can be difficult to clean - and can't imagine many of us being mistaken for the Sky boys if you go with black & blue though!
posted by Blackhound [466 posts]
29th January 2010 - 23:45
well, as it's all my suggestions in one jersey i have to go for 'kit6' , it's different to all the "me too" solid-colour-with-a-band-across-the-middle jerseys out there and asymmetrical is very in this year darlings.
another colour, just for some very subtle detailing work would be nice though. a charity band would be a kind thought too
posted by VecchioJo [808 posts]
31st January 2010 - 18:47
Like both Kit 6 and Kit 4 - the blue on Kit 4 is the best colour, but the design of Kit 6 just takes it I think...
posted by step-hent [718 posts]
31st January 2010 - 20:14
posted by step-hent [718 posts]
31st January 2010 - 20:16
Looks bloody aweful.
If there are different colour options I would buy it but not in yucky blue and white!
Ok it's a cycle jersey but I find I order my stuff aboard as it looks so much better than the naff colours made over here.
How about a competition from the users?
posted by Zaskar [137 posts]
31st January 2010 - 23:06
How about a competition from the users?
nothing stopping you posting your own designs here, we'll mock 'em up
posted by Dave Atkinson [7854 posts]
1st February 2010 - 9:36
Zaskar wrote:How about a competition from the users?
nothing stopping you posting your own designs here, we'll mock 'em up
nothing stopping you posting your own designs here, we'll mock 'em
posted by Oli Pendrey [123 posts]
1st February 2010 - 14:06
I quite like the ones in the thumbnails with are blue on the left and pink on the right. But you can only click in between them... Embrace asymmetry!
two wheels good; four wheels bad
posted by cat1commuter [1448 posts]
1st February 2010 - 20:06
Love the mock up- some people are just too fussy.How many are going to ask " does my bum look big in this". I'll have one when they arrive.
posted by Are We There Yet [53 posts]
2nd February 2010 - 8:56
dave_atkinson wrote:Zaskar wrote:How about a competition from the users?
nothing stopping you posting your own designs here, we'll mock 'em up
nothing stopping you posting your own designs here, we'll mock 'em
Sorry guys didn't mean to sound harsh or mock you guys!
I find the design fine but light blue? how about using colours that are seen by drivers like red or yellow?
Ok I know not all colours are suited for everyone and I would still buy it as long as it is not Brown!
I will come up with a drawing you can mock/up soon! B)
What program will let me replace the colour blue with red or yellow?
posted by Zaskar [137 posts]
17th February 2010 - 0:10
posted by vanderlay [52 posts]
22nd September 2011 - 10:28


text:
Syrian refugee shoes
When the founder of baby brand JoJo Maman Bébé, Laura Tenison MBE, travelled through Lebanon and along the Syrian border last week, she asked locals and refugees what would make the most difference. The answer was that - as well as being in desperate need of essential basics - with the cold weather approaching, warm shoes and boots were one of the most pressing needs.
- Katie Price Harvey
- Visits from friends and family 'ward off depression in later life'
- Cannabis 'safe to treat pain' but no proof it helps
- Halloween costumes
- #MightyandMedicated
- Stillborn photos
Buy & Sell
Meet a mum
Sign up to Netmums.com and meet other local mums looking to make new friends in Widnes.
Family health interactive guides
Healthy home check Take this test to find out whether your home could be a health risk.
Healthy child self-assessment Find out how you and your child are doing with this short quiz.
Fitness self-assessment Take this simple assessment test to find out how fit and active you are.
Alcohol self-assessment Assess the effects of your drinking and find out if you're drinking too much.

subset: commoncrawl


text:
Photo Gallery
Capitol enlivened by children's event
Bruce Asato
3 of 10
Kai'ea McKillop, 5, from Kaimuki, tries his hand at tennis for the very first time during the 20th annual Children and Youth Day on the grounds of the Hawaii State Capitol and The Frank F. Fasi Civic Center, including Iolani Palace, Punchbowl St., King Kamehameha V Judiciary History Center, Kalanimoku Building. Hundreds of people took part in various activities, rides and games, visited educational displays and enjoyed musical presentations and food booths.
Latest Galleries
Photos: FB: UH vs. Boise State, Oct. 3
Photos: VB: UH at UC Riverside, Oct. 3
Photos: Windward Hoolaulea, Oct. 3
More Photo Galleries »


text:
CROSSTOWN HARMONY CHORUS. Sweet Adelines chorus invites women of all ages, who love to sing, dance and perform, to audition for membership from 7 to 9:30 p.m. on June 13 and 20 at Gonzaga Hall, St. Aloysius Church, 92nd St. and Greenfield Ave., West Allis. Information: (414) 271-9116, or visit crosstownharmony.org.
IDEOGRAM FILMS. Two females ages 20 to 30, one male age 20 to 30, and two males ages 40 to 60 are needed for short film "Pretty Girl." This project is intended for film festivals and will be produced in the Milwaukee area July 18-24. Auditions will be held the week of June 20 and are open to union and nonunion performers. Auditions will consist of reading from provided monologue and sides. Compensation will be a day rate, meals, credit and copy of the final project. Send résumé and a recent headshot to: email@example.com.
MILWAUKEE CHILDREN'S CHOIR. Children in grades 1 through 8 are invited to enroll for the 2011-'12 season. The Prelude choir (grades 1-3) does not require a formal audition. Higher-level choirs (grades 4-8) require an audition that includes singing and a musical evaluation. No previous choral or musical experience is required, but the child should enjoy singing. Information: (414) 221-7040, or email firstname.lastname@example.com.
OVER OUR HEAD PLAYERS. Six to 10 men and women ages 20 to 50 are needed for "Do Not Disturb." Auditions will be held at 7 p.m. June 20 at the Sixth Street Theatre, 318 6th St., Racine. Auditions will consist of cold readings from the script; prepared pieces are not needed. No previous theatrical experience is necessary. Information: (262) 632-6802.
VILLAGE PLAYHOUSE OF WAUWATOSA. Directors are needed for the 2011-'12 season. Positions are available for the following shows: "Steel Magnolias," "The Best Christmas Pageant Ever" and "Murder at the Vicarage." Those interested should submit a résumé and cover letter listing the show(s) you'd most like to direct to: VPW, P.O. Box 13095, Wauwatosa, WI 53213. The deadline is July 15.
Audition notices may be mailed to: Auditions, Entertainment Department, Milwaukee Journal Sentinel, Box 371, Milwaukee, WI 53201; faxed to (414) 224-2133, or emailed to email@example.com (please put "Audition " in the subject field). Information must be received two weeks before publication.


text:
It looks like e-book lovers around the world can definitely look forward to tomorrow's expected iPad mini reveal, as paid iBookstore content has just gone live in 17 new countries. If a smaller iPad is indeed in the cards -- and we're pretty sure it is -- it will compete directly with Amazon's Kindle line. Expanding the company's e-book offerings might be a good indication of how Apple will angle its announcement to appeal to would-be Kindle buyers.
The full list of countries getting refreshed iBookstore content is as follows: Argentina, Bolivia, Brazil, Chile, Colombia, Costa Rica, Dominican Republic, Ecuador, El Salvador, Guatemala, Honduras, Mexico, New Zealand, Nicaragua, Panama, Paraguay, Peru and Venezuela.
This news also matches up nicely with the recent discovery of titles in the iBookstore referencing the as-yet unannounced iBooks 3.0 platform in their descriptions. Apple launching a smaller iPad with a focus on reading and education is something our own Erica Sadun is already betting on, and these two clues seem to support that theory quite nicely.
- Key specs
- Reviews • 4
- Prices
- Discussions
- Form factor Tablet
- Operating system iOS (8)
- Screen size 7.9 inches
- Storage type Internal storage (16 GB, Flash)
- Maximum battery life Up to 10 hours
- Dimensions 7.87 x 5.3 x 0.29 in
- Weight 0.73 oz
- Announced 2014-10-16


text:
tata steel ltd
(TATA:National Stock Exchange of India)
Last 251.10 INR
Change Today 0.00 / 0.00%
Volume 0.0
TATA On Other Exchanges
As of 7:06 AM 10/9/15 All times are local (Market data is delayed by at least 15 minutes).
BOARD MEMBERS AFFILIATED WITH Karl-Ulrich Koehler *
*Data is at least as current as the most recent Definitive Proxy.
|Recently Viewed
MOST CONNECTED PEOPLE TO Karl-Ulrich Koehler
|Andrew M. Robb
|0 Relationships
|Bushen Lal Raina
|0 Relationships
|Cyrus Pallonji Mistry
|0 Relationships
|Dinesh Kumar Mehrotra
|0 Relationships
|Ishaat Hussain
|0 Relationships
Sponsored Financial Commentaries
Sponsored Links
Report Data Issue
To contact TATA STEEL LTD, please visit . Company data is provided by Capital IQ. Please use this form to report any data issues.
Please enter your information in the following field(s):
Your requested update has been submitted
Our data partners will research the update request and update the information on this page if necessary. Research and follow-up could take several weeks. If you have questions, you can contact them at email@example.com.


text: We booked this hotel the morning of our arrival and got the Honeymoon suite for about half price. Pros : - Staff. They were quite friendly. Our Ipad burned-out our first night at the hotel and it was entirely booked the next day. Without access to wifi/internet we were in a bit of a pickle but Angel at the front... More


text:
Pontiac Dealers Dallas, TX
Showing Pontiac dealers within 25 miles of Dallas, TX Edit search
Authorized Pontiac Dealers
1. Autoflex Leasing 11.9 miles
558 S Central Expy
Richardson, TX 75080
"Dealer continues to advertise car they DO NOT Have. I even called and made an appointment. The salesman admitted the car I called about had been sold for months. I also contacted there internet sales and complained. Now 3 mts later they are STILL advertising a Z4 they don't have. "
Dealers With Used Pontiac Inventory
1. Lightning Motorsports 14 miles
1100 W. Pioneer Pkwy
Grand Prairie, TX 75051
"Great experience with these people. "
2. Broadway Auto Group 7.8 miles
10501 Harry Hines Blvd
Dallas, TX 75220
"Max was great to deal with. No pressure and no hassles. "
3. DFW Car Mart 16.5 miles
2020 E Division St
Arlington, TX 76011
"Kenneth was great to work with. He got us financed when no one else would in a newer Honda Accord then what we were looking at. No hassle at DFW CAR MART!!!!!!"
4. Shawnee Motor Company 6.7 miles
3250 Oradell Lane
Dallas, TX 75220
"Me and my wife was very happy with the dealer. He is very nice and has good quality cars was very impressed. I almost turned around because of the area. But I'm glad I didn't the man keeps his cars inside a building. He has a good business back there. Recommend him to all my associates will buy from him again. Once you see the quality of cars he has you will not be disappointed"
5. James Maverick Motor Sports 13.5 miles
1628 W Crosby Rd, #109
Carrollton, TX 75006
"Good communication. Have not bought anything yet but responses and answers to my questions were addressed quickly."
Show all 114 dealers
Find a Dealer
Sell My Car
- It's free.
- Reach over 15 million shoppers instantly.
- Free advanced pricing and tracking tools.
- Craigslist posting tool.
Popular Used Cars
Used Pontiac G6
245 Great Deals out of 6,041 listings starting at $1,800
Used Ford F-150
2,711 Great Deals out of 60,124 listings starting at $1,450
Used Chevrolet Silverado 1500
2,112 Great Deals out of 49,362 listings starting at $999
Used Toyota Camry
1,277 Great Deals out of 37,733 listings starting at $1,450
Used Nissan Altima
1,119 Great Deals out of 35,578 listings starting at $400
Used Ford Focus
1,281 Great Deals out of 33,998 listings starting at $1,200
Used Ford Fusion
1,165 Great Deals out of 32,499 listings starting at $1,950
Used Chevrolet Impala
1,573 Great Deals out of 32,481 listings starting at $1,800
Used Ford Escape
1,390 Great Deals out of 32,090 listings starting at $750
Used Honda Accord
1,125 Great Deals out of 31,511 listings starting at $1,491
Used Chevrolet Malibu
1,061 Great Deals out of 28,655 listings starting at $1,195
Used Toyota Corolla
906 Great Deals out of 25,738 listings starting at $2,431
Used Jeep Grand Cherokee
1,095 Great Deals out of 25,334 listings starting at $1,488
Used Hyundai Sonata
947 Great Deals out of 24,623 listings starting at $1,595
Used Chevrolet Equinox
1,001 Great Deals out of 23,967 listings starting at $1,950
Used Ford Explorer
904 Great Deals out of 23,868 listings starting at $587
Used BMW 3 Series
1,039 Great Deals out of 23,496 listings starting at $1,500
Used Ram 1500
1,058 Great Deals out of 22,161 listings starting at $8,987
Used Jeep Wrangler
642 Great Deals out of 21,355 listings starting at $3,000
Used Honda Civic
814 Great Deals out of 20,907 listings starting at $399


text: prisons Michigan severing ties with embattled prison food vendor By Kevin Lavery • Jul 14, 2015 The state is cutting ties with Aramark and starting fresh with a new company to provide food service to Michigan prisons. Current State talks with Michigan Public Radio Network Statehouse Bureau Chief Rick Pluta. Listen Listening... / 12:43 Huffington Post reporter says MI, US prisons need more transparency By April Van Buren • Jul 6, 2015 http://www.dliebelson.com/ Current State talks with Huffington Post reporter Dana Liebelson about her recent article about juvenile offenders in Michigan's prison system and the challenges facing reporters covering the prison system. Listen Listening... / 13:19 MI prosecutors urge caution on prison reforms By Kevin Lavery • Jul 2, 2015 Michigan prosecutors have issued a report with their positions on reforming the state's prison system. Listen Listening... / 13:56 New report says MI can safely reduce prison population by 10,000 By April Van Buren • Jun 18, 2015 Current State talks with Barb Levine, lead author of a report from The Citizens Alliance on Prisons and Public Spending that offers a roadmap to reducing the prison population and saving the state hundreds of millions of dollars every year. Listen Listening... / 12:58 Reporter details juvenile offenders' lawsuit alleging prison rapes By April Van Buren • Apr 20, 2015 Flickr - Ben Listen Listening... / 12:20 From 2003 to 2013, Michigan prosecuted over 20,000 juvenile offenders as adults. Advocates for juvenile justice reform say youth housed in adult prisons are at a much greater risk for sexual abuse and suicide than the adult prison population. And while there is now a sight and sound barrier between juvenile offenders and the adult prison population, that wasn't always the case. The state is now facing allegations from seven former juvenile offenders that they were sexually abused by both other prisoners and prison staff while housed in those facilities. Pages1 2 3 next › last »


text:
Fairfield Village Green • Saturday, December 5, 2015 • 3:00 to 6:00 pm
Light Up Fairfield will ring in the holiday season at the Community Arts Center and all of Village Green including the Fairfield Lane Library and the Waterford. Help Santa welcome the holiday season and light up the big tree on the Village Green Amphitheater Stage.
Below is the schedule from 2014's Light Up Fairfield. We will update this page with the 2015 schedule as it gets set.
Holiday Market
Vendors offer their wares so you can get a start on your holiday gift shopping (or a little something for yourself!). Below are participating vendors. Check back as the event approaches for a complete list of vendors.
The Night Before Christmas
Location: Fairfield Lane Library
"ArtReach": A Division of the Children's Theatre of Cincinnati brings this best-loved holiday story to life. Based on the enchanting poem by Clement Clark Moore, this lyrical adaptation celebrates the joy of family and the magic of holiday stories, reminding everyone what Christmas is all about. Space is limited, please call 858-3238 or visit www.lanepl.org to reserve your space.
Christmas Ornament
Location: CAC Art Studio
Fee: $3 per ornament
Holiday fun for the whole family! Make memories painting ornaments that will be treasured and kept as keepsakes. This event takes place during Light Up Fairfield and does not require pre-registration. Fee includes all materials to complete one ornament.
Gingerbread Families
Location: Fairfield Lane Library
All Ages
Saturday, December 6th from 2:00 to 3:00 pm and 3:00 to 4:00 pm
Sunday, December 7th from 2:00 to 3:00 pm and 3:00 to 4:00 pm
Create your very own Gingerbread Family. Each person will receive supplies to decorate a gingerbread man. Space is limited, please call 858-3238 or visit www.lanepl.org to reserve your space.


text:
Skater : Romain Jorda
Post-prod. : Tristan Badual
Réal : Xavier Lanquet et Charles Martiny.
Here's a short interview of Mark Ronson just after playing in La Cigale, Paris...
Filmed and interviewed by Charles Serre
Edited by Charles Martiny
Speak Real Video Magazine interviews Ray Barbee in Paris.
Filmed by Charles Serre
Edited by Charles Martiny
More stuff from "2011"
16 Videos
1 Follower
1 Moderator
Channels are a simple, beautiful way to showcase and watch videos. Browse more Channels.
TM + © 2015 Vimeo, LLC. All rights reserved.
Made with k in NYC.
Your content preferences apply to any Vimeo videos you view. Learn more about content preferences in our FAQ.


text:
I'm using the Jquery decimal mask plugin from Stefano Stypulkowski. It works fine in my project in Chrome and Firefox but it doesn't work in IE8.
I'm getting the error: Object doesn't support this property or method decimalmask.js, line 17 character 9.
I have the following scripts included:
<script src="/Scripts/jquery-1.4.4.min.js" type="text/javascript"></script>
<script src="/Scripts/jquery.caret.1.02.min.js" type="text/javascript"></script>
<script src="/Scripts/decimalmask.js" type="text/javascript"></script>
How on earth do I go about finding out what is the problem when this works fine in FF and Chrome? Its not my code and neither am in a position to start debugging plugin code. Can anyone offer any advice how to resolve this problem? I don't really want to have my project not work in IE but in other browsers. This kind of thing drives me crazy!


text:
What's New
Doze and App Standby
New battery-saving features Doze and App Standby are described in Power Management while the remaining Power section has been revised.
An entirely new Authentication section describes the Fingerprint, Gatekeeper, and Keymaster interfaces in detail, including a list of Keymaster features and an implementer's reference.
Adoptable Storage
The Storage section has been revised to introduce adoptable storage that can hold app data and distinguish between it and traditional storage.
Instructions now exist for configuring runtime permissions, visual voicemail, and Android's new file system. And carriers get custom configuration.
Information on the Jack (Java Android Compiler Kit) default toolchain in Android 6.0 is available, as well as instructions for debugging garbage collection in ART.
The Media section now contains descriptions of media resource manager dependencies for OEMs and SoC vendors.
Bluetooth Stylus
The Accessories section now explains how to implement a Bluetooth Stylus.
Getting Started
Explore the Source
Get the complete Android platform and modify and build it to suit your needs. You can also contribute to the Android Open Source Project (AOSP) repository to make your changes available to everyone else in the Android ecosystem.
Port Android to Devices
Port the latest Android platform and create compelling devices that your customers want.
Build Accessories
Sometimes, a device can't do it all. Tap into Android's open accessory standard and build accessories to complement the wide variety of Android-powered devices.
Get Compatible
Being Android-compatible lets you offer custom features but still give users and developers a consistent and standard experience across all Android-powered devices. Android provides guidance and a test suite to verify your Android compatibility.
Help this Site
Use the Send Feedback button at the bottom of any page to request improvements to the content or identify errors. In addition, source.android.com is maintained in the Android Open Source Project. See the docs/source.android.com project log in AOSP for the complete list of changes to this site. Contribute your own updates to that same project and help maintain source.android.com.


text:
The Lodge At Bretton Woods
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
How has this hotel rated in the past?
- Shuttle bus service
- Free High-Speed Internet
- Swimming pool
- Wheelchair accessibility
- Business Services
- Free parking
Travelers also recommend:
- More recommendations
TripAdvisor Reviews The Lodge At Bretton Woods
Travel Blogs from Bretton Woods
Maine Back to New Hampshire
... br>
We stopped off ...
Live Free or Die Hard
... with the group at Cold Hollow Cider Mill. Had a nice lunch there and checked out the shop. Then we headed a few miles away to Ben and Jerry's!
The tour of Ben and Jerry's ice cream factory near Burlington, VT was fantastic. Very informative; we got to see a lot of great marketing and business ideas from a company that's been doing things right for over 40 years. The business teacher in me got a lot out of it ...
We're on top of the world!
My dad had already earned the sticker proclaiming "This car climbed Mt. Washington" on a previous trip, but he wanted to get back to the top to share the experience with Tuukka the puppy, and myself.
Tuukka had a feeling this was going to be an exciting trip and barked enthusiastically at the booth attendant at the base of the mountain road.
Once the pleasantries were exchanged we were off and up the mountain, traveling on a road that at times could ...
When exactly does a walk become a hike?
... you call it?". Disappointingly, when looking it up, the dictionary definition only states 'a long walk, especially in the country or wilderness' with no further qualification regarding distance or requirements for rescue. So the jury's still out on that, really.
After dinner, Nay and I had real trouble sleeping, possibly from all the exciting hike chat. We did have a very productive evening though with blog posts written, ...
North Conway - New Hampshire
... cases now). The manager evened help us carry our cases up to the 7th floor where our car was, showed me what side I need to get into the car, a detailed printed guide of how to get out of Boston, gave me her mobile number in case we had any problems ( at this point I felt like saying I'm a happily married man but I think she was just being extremely helpful)?
They even gave us a detailed direction sheet how to get out of Boston and onto the road we required.


text:
Narrow your search for
Elk Lighting...
Elk Lighting...
External Ads:
Open Box Deals
Up to 80% Off
10% Off Top Brands!*
Top Elk Lighting
$132.25 $362.99 In Stock
Sterling 35x22" Mirror with Shutters and Mirrored Arch Natural Aged Wood 137013
$198.00 $296.99 In Stock
Dimond 33"h Belvior 2-Light Park 3-Way Table Lamp Aria Bronze And Iron D1518
Still searching for Elk Lighting?
Live Chat with an Expert and we'll be happy to help you search.
Or try expanding your selection in ...
Brand: Show more than just elk lighting
Brand: Show more than just elk lighting
Also see related categories below, or view our Full Site Map


text:
- 88Promote
Framed Art Print
Forest Creatureby ErDavid
Choose from a variety of frame styles, colors and sizes to compliment your favorite Society6 gallery, or fine art print - made ready to hang. Fine-crafted from solid woods, premium shatterproof acrylic protects the face of the art print, while an acid free dust cover on the back provides a custom finish. All framed art prints include wall hanging hardware.
stunning work!
cool work!
Thanks Netty, Fabian, Gwenola and Joke I'm glad you liked it!
Gorgeous work
Thanks Condor & Maggs!
So cool!
Thank you Alberto!
this is awesome!
Thank you Matt! :-)
woow David, this is just amazing ♥ post more of these!!!!!
great David !!
Thank you Nika!
Carina I'll try
Nice! great talent you got!
gorgeous, mysterious, fires my imagination.
Awesome work
Very nice
Very good!
See More Comments


text:
Honoring Lt. Michael Murphy
The Patriot Guard Riders, a nonprofit group that attends the funerals of fallen soldiers, presented the parents of Lt. Michael Murphy, of Patchogue, with a portrait of their son at the Northport VA Medical Center on March 17, 2014. Murphy, Navy SEAL, received a posthumous Medal of Honor for heroism in Afghanistan, (Credit: James Carbone)
Newsday.com now uses Facebook for our comment boards. Please read our guidelines and connect your Facebook account to comment.
West Islip homecoming king and queen competition Port Jeff students learn about chainsaw art Under the helmet: Inside LI football helmets FDR's son's home for sale in Woodbury 3 injured in police patrol car crash Crash closes Sunrise Highway in East Patchogue Off-duty Suffolk County officer helps revive hockey player North Amityville gas station attendant robbed, say cops One dead after bar dispute 'Blessing of the Animals' in Brentwood Making apple cider in Northport Tom Cutinella anniversary vigil on football field


text:
i looked at the first chapter of this book and it all confused me because of the accents
wouldnt the accents be defined by the word? because sure as i am alive (questionable) they arent written all over this site and everything else
It is currently Fri Oct 09, 2015 3:17 am
Say, Ulpianus, do you propose that Caesar's writing is, for the modern Latinist, merely functional?
I'm a fan of him myself. Brilliant, impeccable Latin, and, really, not hardly as boring as some, who've perhaps read the first few lines only, make it out ...
Total posts 108285 • Total topics 13676 • Total members 20209


text:
Homeowners' insurance isn't a luxury, it's a necessity. In fact, most mortgage companies won't make a loan or finance a residential real estate transaction unless the buyer provides proof of coverage for the full or fair value of the property (most of the time this is the purchase price). Read on to learn how you can make sure your homeowners' insurance will meet your needs.
Homeowners' insurance isn't a luxury, it's a necessity. In fact, most mortgage companies won't make a loan or finance a residential real estate transaction unless the buyer provides proof of coverage for the full or fair value of the property (most of the time this is the purchase price). Read on to learn how you can make sure your homeowners' insurance will meet your needs.
Maintain A Security System
A burglar alarm that is monitored by a central station, or that is tied directly to a local police station, can help lower a homeowner's annual premiums by 5% or more. To obtain this discount, the homeowner will typically be required to provide the insurance company with proof of central monitoring.
Install Additional Smoke Alarms
Smoke alarms are another way to reduce your homeowners' insurance premiums. While these are standard in most new houses, installing them in older homes can save the homeowner 10% or more in annual premiums. Some insurance companies will also give further discounts if the you install additional smoke detectors. In addition to premium discounts, in case of fire, they could save your life!
Raise Your Deductible
Like health insurance or car insurance, the higher the deductible the homeowner chooses, the lower the annual premiums will be. The catch? Smaller claims such as broken windows or damaged sheetrock from a leaky pipe, which typically will cost only a few hundred dollars to fix, will most likely be absorbed by the homeowner.
Look For Multiple Policy Discounts
Many insurance companies give a discount of 10% or more to customers that maintain other insurance contracts under the same roof, such as auto or health insurance. Consider obtaining a quote for other types of insurance from the same company that provides your home insurance. You may end up saving on both annual policy premiums.
Plan Ahead For Construction
If you are planning to build an addition to the home or a structure adjacent to the home, consider the materials that will be used. Wood-framed structures are highly flammable and will cost more to insure, whereas cement- or steel-framed structures will cost less because they are less likely to succumb to fire or adverse weather conditions.
Beware Of Costly Recreation
Pools and other potentially injurious devices such as trampolines can drive annual insurance costs up by 10% or more. This may seem like a small price to pay given the joy these items bring, but it is still something that should be considered by the homeowner prior to purchase.
Pay Off Your Mortgage
Obviously, this is easier said than done, but homeowners that pay off their mortgage debts will most likely see their premiums drop. Why? The simple reason is that the insurance company figures that if you own the home outright, you'll take better care of it.
Make Regular Policy Reviews
It is a good idea to review your insurance policy at least once a year and compare the costs of other insurance policies to your own. In addition, you should make note of any changes that have occurred that could lower your premiums, including the addition of a burglar alarm, sprinkler system, or disassembly of the trampoline. Neighborhood changes could also affect your rates, such as the addition of a fire hydrant within 100 feet of the home.
Guaranteed Replacement Value Insurance
All homeowners should buy "guaranteed replacement value" homeowners' insurance. This means that your home will be rebuilt in the event of a disaster, no matter what the cost. Because the cost of building a new home tends to increase over the years, guaranteed replacement value policies will absorb the increased costs and provide the homeowner with a cushion.
Get An Endorsement
Legally speaking, an endorsement is an amendment to the basic homeowner's policy. For example, a woman wanting to insure her diamond ring would obtain a formal appraisal from a jeweler, then send the appraisal to the insurance company for a special notation on her insurance contract. This expedites the claims process and ensures that the homeowner gets the full dollar value of the item if it is lost, stolen or damaged in a disaster.
Related Articles
Investing Basics
What Does In Specie Mean?In specie describes the distribution of an asset in its physical form instead of cash.
Calculating Cross Elasticity of DemandCross elasticity of demand measures the quantity demanded of one good in response to a change in price of another.
Fundamental Analysis
Emerging Markets: Analyzing Colombia's GDPWith a backdrop of armed rebels and drug cartels, the journey for the Colombian economy has been anything but easy.
6 Cost-Effective Tips for Raising Your First ChildThe excitement of welcoming your first child to your family shouldn't prevent you from making good cost-effective decisions.
5 Ways to Date on a BudgetDating on a budget doesn't have to be boring. Try these 5 tips to find the best dates on a budget.
7 Kids Items You Should Never Buy UsedBuying secondhand items is a great way to save money, but these seven kids items should not be bought used.
10 New Apps That Help Budget For Expensive CitiesFrom platforms for saving money to those that account for side jobs, mobile apps are changing spending habits and income generation in urban areas.
How Cooking At Home Can Save You Real DoughCooking at home saves time and money but most importantly, it could even help lower future health costs.
Personal Finance
Money Matters on Campus: Attitudes & AptitudesFinancial trends among college students are a cause for concern, prompting a renewed emphasis on financial instruction.
Home & Auto
Why Housing Costs Shouldn't Exceed 30% of Your BudgetFinancial experts will argue that there's no problem with allocating 50% of your net income to housing, but that barely leaves enough money for living comfortably. Reducing housing expenses to ...


text:
SKYRC 6X80+ Bluetooth Balance Charger / Discharger / Lithium Battery Meter / Motor RPM Tester
charger allows user to change the end voltage. Lipo Battery Meter The user can check battery's total voltage, the highest voltage, the lowest voltage and each cell's voltage
free shipping
(1 Review)
» Batteries & Chargers
Add To Cart
Mini Camera Style White Light Plastic Keychain - Red + Black + Silver (3 x AG13)
Color Red + black + silver Material ABS Quantity 1 Emitter Number 1 LED Color White Battery 3 x AG13 included Functions Great decoration for your cellphone or bag; Retro
» LED Keychains
Digital Photo Frame w/Mini USB/3.5mm Audio Jack/SD/CF (800 x 480px/2GB)
, calendar
- Built-in 2GB flash memory
- Supports SD MMC MS CF memory card up to 16GB
- Supports JPEG, JPG format image
- Remote control range: 5 meters
- Interface: SD + CF + USB
» Digital Photo Frames
MOCREO Ultra-Portable Wireless Bluetooth V3.0 Speaker w/ Built-in Battery / Microphone 3.5mm Aux-in
Brand N A Model MOSOUND MAGIC Quantity 1 Color Black Bluetooth Version V3.0 Operating Range 10 meter Compatibility MP3 MP4 Iphone Ipad Smartphone and Bluetooth V3.0
(4 Reviews)
» Bluetooth Speakers
TS-BTDF01 Bluetooth V4.0 Digital Transmitter w/ Optical, Coaxial, 3.5mm Output - Black
; Maximum distance of up to 20 meters; HiFi level APTX lossless analog audio launch; Multi-interface audio input using a wider range; Dimensions: 9.1 x 5.7 x 2.5cm; Weight bare
(0 Review)
» Other Bluetooth Devices
BTS-19 Convenient Water Resistant Bluetooth Speaker w/ Hook + Handsfree Call + FM - Green + White
Hour Standby Time: 72Hour Music Play Time: 8~10 Power Supply: DC 5V USB power Other Features: Press the power switch to shift between bluetooth and radio.
BTS-19 Convenient Water Resistant Bluetooth Speaker w/ Hook + Handsfree Call + FM -Deep Pink + White
Hour Standby Time: 72Hour Music Play Time: 8~10 hours Power Supply: DC 5V USB power Other Features: Press the power switch to shift between bluetooth and radio.
Brand N A Model MOSOUND MAGIC Quantity 1 Color White Bluetooth Version V3.0 Operating Range 10 meter Compatibility MP3 MP4 Iphone Ipad Smartphone and Bluetooth V3.0
Blue Tiger BLK-M29 Bluetooth v2.0 Headset w/ Charging Dock - Black + Silver
Operating Range 10 meter Compatibility Compatible with Bluetooth v1.2 and v1.1 Microphone Yes Power Supply Built-in Battery Compatible With Iphone 5 PS3 and other cell phones
» Bluetooth Headphones
Leadsound i6BT Wireless Bluetooth 2.1 Speaker w/ Microphone - Black + Silver Grey
meter Compatibility All devices with Bluetooth function Microphone Yes Channels 2 Total Power 3W Impedance 4 ohm Signal to Noise Ratio SNR =82dB Sensitivity 86dB Radio N A
DRF1605H UART Serial Port to Zigbee Wireless Module Adapter w/ Antenna - Green
directly drive; RS232 chip can directly drive; Can directly drive USB to RS232 chip Application Learning board, development board Other Zigbee network typically consists of
» Transmitters & Receivers
H301 Portable 48MHz USB Digital Oscilloscope
- Also can be used as a frequency meter
- Sampling rate: 48MHz
- Voltage measurement range: + - 80V
- Vernier measurement: Voltage pulse width
- Real-time display: Average
» Oscilloscope & Logic Analyzer
Lefant FS-01 Rechargeable 2.4GHz Wireless Air Mouse Red Laser Pen / Presenter w/ Gyroscope - Black
gyroscope - 2.4GHz wireless - USB 2.0 mini receiver - Built-in lithium battery by USB - Red indicator for power, it is flashing when power shortage and on when charging; It is
» Presenters
G05-A USB Rechargeable 3D Active Shutter IR & Bluetooth Glasses for TV - Black
under 3D output mode; While searching 3D signal, please keep 3D glasses right in front of the 3D TV about one meter; As power off status, press the power button and then
» 3D Glasses
Rechargeable Waterproof Bark-Control Dog Collar with 0.9" LCD Remote Controller
and a range of 300 meters in open areas. Many great control methods are built-in like static or pulse and vibration and sound with 100 levels of adjustment. It has a mild
» Pet Outdoor
Kemico HIFI Air Music Box Wi-Fi Power Amplifier for iOS Android Windows Phone / Tablet - White
Supply: 5V 2A Power Adapter: USB Certification: CE, EMC, R&TTE, RoHS, Wi-Fi Other Features: Wi-Fi: Realtek RTL8188 2.4Ghz IEEE802.11n 150 Mbps b g n compatible ;
» Amplifiers
HD-215 1.6'' LCD Screen Bluetooth V2.0 Wrist Watch w/ Microphone - Black
Model HD-215 Quantity 1 Color Black Bluetooth Version 2 Operating Range 5~10 meter Compatibility Cellphone,computer with bluetooth Microphone Yes Radio No Supports Music
» Smart Watches
LINK-490 Bluetooth v4.0 Anti-Lost Alarm Tracer Device - Blue
Brand N A Model LINK-490 Quantity 1 Color Blue Bluetooth Version Bluetooth v4.0 Operating Range 20 meter Compatibility Compatible with Bluetooth v2.0 Microphone No Radio
GL-025 2.45" LCD Digital Blood Pressure Monitor / Sphygmomanometer w/ MP3 Player / FM / Alarm Kit
music playing; Loudspeaker for teaching guide: made of class DS chip, big power and clear sound, can cover 400 square meter space; FM radio: high sensitivity w many channels
» Blood Pressure Meters
BSV-SL030 Solar Power 3W 7.5lm 7000K 31-LED White / Blue Light Tent Lamp Emergency Light - (2 PCS)
- Battery Light Time: 8~10 hours
- Max Current: 1000mA
- Charge Power Input: 1500mA maximum
- Output: USB output: 5.4V 300~1000mA
- Total Emitters: 30-LED white light + 1-LED
» Solar Powered Gadgets
Lefant SRC-303 2.4GHz Wireless 2000dpi Air Fly Mouse w/ Receiver Combo - Black + Silver Grey
Brand Lefant Model SRC-303 Quantity 1 Color Black + Silver Grey Material ABS Connection Method 2.4G Wireless Connection Type USB 2.0 Operation Distance 10M Tracking
» Wireless Mouse
KLARUS RS1A 7~210lm 4-Mode White Crown Head Flashlight w/ Cree XP-G2 - Black (1 x AA)
Fast strobe Mode Memory Yes Switch Type Forward clicky Switch Location Side Lens Tempered optical glass Reflector Aluminum Textured OP Beam Range 100 meter Strap Included
» AA Flashlights
BK880 Bluetooth V3.0 5.-Key Keyboard w/ 2000mAh Battery Portable Power Supply / Flashlight - Black
computer with Bluetooth HID driver; Bluetooth V3.0, class 2, frequency: 2.4GHz, working current: Packing List 1 x Bluetooth keyboard 1 x Holder stand 1 x Micro USB charging
» Keyboards
MK912 Multifunction Bluetooth v3.0 Headset / Screen Stylus Pen w/ Microphone - Black + Grey + Silver
Brand N A Model MK912 Quantity 1 Color Black + grey + silver Bluetooth Version v3.0 Operating Range 10 meter Compatibility Touch cellphones, tablets Microphone Yes Radio
Subscribe to our newsletter:
© 2006~2015 DX.com. All rights reserved.
Note:Stock and Availability shown on this site is for your reference only. While
we strive to provide the most accurate and timely stock and availability information,
availability information may become out of date and may change between the time
you added an item to cart and the time your order is received.
Quantities on clearance items are limited. Prices are current at time of posting. DX Reserves the right to change prices at any time without notice.
Partners: DX Wholesale Website l
MadeinChina l


text:
There's a famous saying that goes something like this: "You've got to spend money to make money." We sometimes ...On July 15, 2013 / By Martha Buckly
Getting traffic to your website isn't as easy as you think. With millions of blogs on the web, what ...On July 11, 2013 / By Igor Ovsyannykov
If you're running a business, overlooking the design of your Facebook fan page isn't an option. You must have ...On July 9, 2013 / By Igor Ovsyannykov
AdSense is an onscreen advertisement service offered by Google which allows users to publish advertisements on their websites and ...On July 9, 2013 / By Alice McLean
Blogging can be a fun creative outlet or a profitable occupation. A blog can show the world your thoughts ...On June 27, 2013 / By Andrew Lisa
Nowadays, doesn't it seem like there are just too many blog consultants out there? Have you already started your ...On June 19, 2013 / By Chad Goulde
Burnout has become a hazard for initially enthusiastic bloggers. Yet without consistently fresh and inspired blogs, the blog could ...On June 10, 2013 / By Igor Ovsyannykov
Just as learning is a lifelong process, so is the road to bettering yourself as a writer. Even the ...On June 5, 2013 / By Kevin P
© 2015 Inspirationfeed. All rights reserved.


text:
For every enterprise – including federal government agencies – the shift of critical mass from PCs to mobile has changed all the rules of engagement in just a few short years. More than half of American adults have smartphones and more a third use tablets, according to recent research. That means we are quickly approaching the point where more citizens will access government websites and services via mobile devices than through any other means.
It is easy to discern why mobile devices have become so pervasive. The always-available access of mobility and the overflow of work into our personal lives have pushed these devices to the forefront. Mobility has become an expectation.
Because government has intense, inherent security needs, it has lagged behind the private sector in formulating and implementing mobility plans. But the longer federal agencies postpone going mobile, the greater their risk that constituents will perceive them as trailing in providing basic services.
Mobility's benefits are not limited to good customer service and end-user convenience. Citizens, recognizing that mobility makes their lives and the businesses they deal with more efficient, understand that mobility also will streamline government and reduce its costs. That's important in an environment of shrinking government budgets.
Government agencies also face internal "go-mobile" pressures. Employees who come from the private sector or from college are accustomed to mobile use and expect to be able to leverage these devices to perform their jobs. When the phone issued at work is highly restricted or lacks broad capabilities, government employees frequently use unauthorized tools or turn to their own smartphones to create on-the-job efficiencies.
These practices inadvertently introduce the very risks agencies have tried to avoid by moving slowly into mobile adoption. Employees who use personal mobile devices to perform work may be storing government data without encryption or other security technologies. If the worker's smartphone or tablet is lost or stolen, goes in for repair or travels outside the country with the employee, the agency cannot easily safeguard data stored on the device.
Further, as Americans' public and private lives continue to merge and employees increasingly use personal phones for work-related purposes, government agencies are encountering questions about what authority they may have over employee devices. Say, for example, that a public employee has both government data and photos of her daughter's wedding on her personal mobile device. If that device is deemed insecure, and the affected agency wants to wipe it, what happens to the photos? Who has the right to wipe the phone, and is the employee entitled to prevent it?
The reality is that people are doing business on their mobile devices. Almost every government entity faces the resulting security, efficiency and constituency demand issues, and the speed of technological change permits no time for them to overanalyze. How agencies navigate through the concerns will be increasingly difficult unless they commit to going mobile, put a plan in place and execute that plan.
An approach that may help an agency formulate a plan for addressing mobile is to prepare one or more surveys to help identify mobile priorities. Without being complex or overly broad, surveys will help agencies determine how employees and citizens want to use their mobile devices to interact and access information. Based on survey results, agencies can prioritize their mobile activities – whether mobile device management, website accessibility, services to mobile-enable or something else – to respond to employees' and constituents' pressing needs.
For government, mobile implementation is unquestionably difficult. Yet, it also is essential. There is no one-size-fits-all solution. Going mobile will require a great deal of thought and preparation. But with boldness, agility and a thoughtful approach to planning and surveying, agencies can leverage mobility's benefits to streamline workflow, enhance security and better meet constituent and employee needs.
About the Author
Nolan Jones is Director of eGovernment Innovation for NIC Inc. (NASDAQ: EGOV), the nation's premier provider of official government Web portals, online services and secure payment processing solutions for more than 3,500 local, state and federal agencies across the United States. You may reach him at firstname.lastname@example.com. More information about NIC is available at www.egov.com.
E-Mail this page
Printable Format
Advanced Search
Meet 100 women and men who are doing great things in federal IT.
Click here for the full list
In the newest Federal Employee Viewpoint Survey, feds show they're feeling better than they have over the past few years – but there's still room for improvement, especially in mission-critical IT.
The General Services Administration's Enterprise Infrastructure Solutions contract will anchor the agency's NS 2020 telecommunications strategy.
House and Senate both approve a measure to maintain funding at fiscal 2015 levels through Dec. 11.
The aviation regulator missed a Congressional deadline to develop rules of the sky-ways for drones.
Agency heads, acquisition officers and even CIOs don't need to be hard-core techies. FCW's four-part look at the expertise that's really required to make federal IT run.
FCW recognizes 14 individuals having an outsized impact early in their careers.
As Steve Kelman returns from a summer of aggressive medical treatment, he considers the all-too-common aversion to short-term sacrifices.
Massive open online courses can offer specialized IT instruction on a flexible schedule and on the cheap. That may not always mesh with government's preference for structure and certification, however.
The federal government craves more cybersecurity professionals. These six schools are helping meet that demand.
While the internal use of mobility may raise security concerns, I think the slowness to accommodate constituent mobility is the larger threat to expedient service. Constituents will access services via mobile in greater numbers, and their mobile activity can be captured and managed securely without any conflict of interest concerns. So I think that is where we can focus on software improvements and greater efficiency - by embracing constituent mobility with open arms.
Most mobile devices have their data backed up in a cloud(s). The government wiping (attacking) a personal device draws attention to the government action allowing a reverse engineered wipe (attack) of government systems.
8609 Westwood Center Drive, Suite 500Vienna, VA 22182-2215 703-876-5100
© 1996-2013 1105 Media, Inc. All Rights Reserved. This copy is for your personal, non-commercial use only.To order presentation-ready copies for distribution to colleagues, clients or customers, visit: www.1105Reprints.com


text:
Videos tagged "experimental"
Big City Orchestra ~ How The West Was Won08:53:20
from BCO's "Western Film Greats" (Roil Noise, 2012) http://www.roilnoise.com/bco-wfg.htm+ More details
Center for Sex & Culture, San Francisco, California, July 16, 2011 Actually this snapshot says it all about our latest performance! More: http://www.eroplay.com/Cave/friction_of_passion_pleasure/index.html+ More details
Center for Sex and Culture, San Francisco, California, April 17, 2010. Ah, if you don't do your best work when you are performing at THE CENTER FOR SEX AND CULTURE where there is a deep base, community, spiritual environment of freedom and exploring, you might reconsider your life work! And the physical theater there is designed to promote a cozy intimacy. This is not to say there are not special issues when you are performing in such an erotic institute of freedom. Like a lot of the people came in with crusts of sexual hip images. These would have blocked going deep within one another. But they very quickly shed these crusts. We then had a cozy room of adventurers who were willing to play softly and be silly together, floating together wherever the performance took us. They took personal responsibility for wanting to go wherever the performance went. This caused the night to be one movement flowing freely from one port of call to the other as if we just discovered a different level of where we were just exploring. Usually I just keep throwing out bits and see what catches. But last night it was so smooth! Of course Tomek's music lubricated everything. But that is always what I depend upon! Last night I may have had one or two or more great plants. That always makes my job a lot easier! But mainly it was the people who came. Actually we ran out of time to get into EROTIC FRICTION. This is why I in my younger days did forty-eight hour performances, and why I do series of performances. More: http://www.eroplay.com/Cave/eroticfriction/index.html+ More details
The Uncomfortable Zones Of Fun, April 201103:05:38
Frank Moore's The Uncomfortable Zones Of Fun, experiments in experience/participation performance Recorded at Temescal Arts Center, Oakland, California Saturday, April 23, 2011 The performance Saturday was like eating at an Italian family restaurant! Courses, in the form of people, kept coming in all night. Actually an audience member was so moved that he disappeared at one point into the street and dragged strangers into the theatre. Two of the strangers got extremely into the tribal community which had been created. Most of the people who came were ready to play, even bringing their own musical instruments [even the woman who is a buyer for the rich (buying literally shit for them!) brought her harmonica]. They cleaned out our three bins of free stuff which we have been carrying around for years! They were ready to pull their weight in the performance. This was especially true for the "autistic" kid and his "caregiver." The boy had a ball playing music joyfully, and he was able to put it into words. It turned out that another guy had a sister who is severely "autistic." All I had to do all night was to keep weaving the threads together and come up with bits like using a timer when a guy said he needed four minutes before he would be ready to take his clothes off. NO PROBLEM! But things were so rich I ran out of time again! I couldn't even follow up with the woman who said I was the inspirational source of her art of sexuality and spirituality [she even said I was one of the Grandfathers (am I that old?)]. Everything was so intense, dense and silky deep, time just ran out. We realized that one of the keys of the series is it is free. This opens it up to people who are living on various edges! I always have kept the price low, flexible, or nonexistent! ------- This is a night of the monthly performance series I have been doing for over two years in Oakland, California. At first I called the series REALITY PLAYINGS. But when people kept yapping about how I threatened their comfort zones, I changed the title to THE UNCOMFORTABLE ZONES OF FUN. For three hours I totally improvise around who comes. So I never know what will happen next. Often it gets very surreal erotic by the end! From the poster: Frank Moore, world-known shaman performance artist, will conduct improvised passions of musicians, actors, dancers, and audience members in a laboratory setting to create altered realities of fusion beyond taboos. Bring your passions and musical instruments and your senses of adventure and humor. Other than that, ADMISSION IS FREE! (But donations are encouraged.) More: http://www.eroplay.com/Cave/uzof/uzof-apr2011/index.html+ More details
The Uncomfortable Zones Of Fun, February 27, 201003:01:23
Frank Moore's The Uncomfortable Zones Of Fun experiments in experience/participation performance Recorded at Temescal Arts Center, Oakland, California Saturday, February 27, 2010. Below is what I wrote after this performance: Ah! How do you measure success in a performance? Maybe when the audience [the people who come to the performance] start crying uncontrollably over releases of blocks that just disappeared just by walking through them with willingness and trust. Or maybe when a young sexy co-ed rocks nude on your lap, arousing all kinds of new possibilities. Or maybe when you attract a wide range of people, all ready to go all the way into the volcano, melting into a cozy little joyful community, willing to be uncomfortable and whatever else it takes to go into adventures of fun, floating on the mood music of Kene-J and a psychology grad student… The community so strong that they [most of them] kept staying, playing together, long after the "performance" was "over", and then most of them went to an apartment of one of them to continue hanging out together! And I'm not even getting into what they said of how the performance effected them! [See the full write-up at the link below for that!] I am just glad I didn't get in the way of the potential of the night! Yep! It was a great deep success! And the series is clearly building, tapping into the young art school student community, among other groups. Ah, yes, very dangerous and subversive! You need to do something for at least a year to start this kind of building. And it goes in waves. The art student came months ago. She went home all shining and open and vulnerable and creative. Her roommates and friends saw the changes in her. So when they came, they were ready to go into it explicitly. This audience was the most explicitly ready in over a year! More: http://www.eroplay.com/Cave/uzof/uzof-feb2010/index.html+ More details
K/A/Y Session 13/02/2011 Free audio download there dogmazic.net/KWAY+ More details
The Passions' Conductor, Part 2 - Build, San Francisco, December 1, 200002:35:26
In this PART TWO of a six hour piece, a highlight is a long juicy sweaty erotic sexy enactment of my poem THE MAGICAL CAVE LOVERS by nude Linda, Teresa and me on the mat [one of the last mat pieces we did].+ More details
Cleymoore (Pluie/Noir, Archipel) @ Spot Bleu Session 0302:31:45
Our third guest, dj & producer Cleymoore (Pluie/Noir, Archipel), as we don't want to sound pretentious will let the music speak for itself. Notre troisième invité, le producteur & dj Cleymoore (Pluie/Noir, Archipel), dans un souci de modestie nous laissons la musique s'exprimer. FREE DOWNLOAD LINK: https://soundcloud.com/spot-bleu/cleymoore-spot-bleu-session-03 Watch it on Youtube: http://www.youtube.com/watch?v=ShlX2X5QPmI Special Thanks to Le Point G for hosting us, Martin Letellier & Improvist CLEYMOORE: https://www.facebook.com/cleymooreoff... https://soundcloud.com/cleymoore PLUIE / NOIR: https://www.facebook.com/pluienoir https://soundcloud.com/pluie-noir SPOT BLEU: https://spotbleu.org https://www.facebook.com/pages/Le-Spo... IMPROVIST: https://www.facebook.com/IMPROVISTPARTY+ More details
The Uncomfortable Zones Of Fun, May 201102:31:02
Frank Moore's The Uncomfortable Zones Of Fun , experiments in experience/participation performance at Temescal Arts Center, Oakland, California Saturday, May 28, 2011 This one will be evermore known to us here as THE OBVIOUS BLOWJOB! This performance was clearly a piece within the larger performance which is the series which has been going on for about two years. It just took off as if we had started where we left off last month. People came back from previous times ready to play, bringing other people who also were ready to play. Then there was the woman who said she came because she heard that somebody sucked a cock at a previous expedition [I neither confirmed nor denied that]. That forced us to jump fully into the meat extremely quickly. Wonder what would have happened if I went for the obvious blowjob instead of going to the mysterious dance of erotic friction which brings up aroused intimacy of going outside taboos. [I have to try going for the obvious blowjob sometime!] So things went surreal and stayed there all night. Everyone participated deeply. I used all my tricks and tools: slides, strobe lights, random readings of my writings, the gestures which I have made more explicit, etc. There were layers of different activities in the room. On one end of the room were Tomek, Shirt, Abigail, etc. playing music joyfully. On the other end were the dancers working through morality, individualism, freedom, going beyond the comfortable acceptable thing, or not! In the middle of the room readers were totally jamming, when they were not playing dress-up. The altered state of awe was only limited by a fast fragile romantic wax which ultimately ended the performance early. But all in all, it was a great night! But what if I had gone for the obvious blowjob? More: http://www.eroplay.com/Cave/uzof/uzof-may2011/index.html+ More details
What are Tags?
Tags are keywords that describe videos. For example, a video of your Hawaiian vacation might be tagged with "Hawaii," "beach," "surfing," and "sunburn."


text:
All the latest on the world's youngest royals
George Clooney made women swoon when he first burst onto the Hollywood scene in the 80s.
But who knew the eternal bachelor would only get better with age?
With a more dapper dress sense, a neater haircut and trimmed eyebrows, he goes to show that men really are like a fine wine.
And he's not the only Hollywood hunk that time has been kind to.
Will Smith, Hugh Laurie and Brad Pitt are all proof that another candle on the birthday cake is far from a bad thing.
Tell us who your favourite is in the Comment box below.
All the latest on the world's youngest royals


text:
Join the 'Menthol' group to help and get support from people like you.
Menthol Support Group
Our support group for Menthol has 12 questions and 7 members. Updated 20 Jan 2015.
Latest News
Dermatologist Offers Advice on Treating Kids' Hives
SATURDAY, July 18, 2015 – If your child develops an itchy rash, it could be hives, experts say. Common symptoms of hives – which are usually temporary and harmless – include: slightly raised, ...
Latest Questions
Can I still get menthol nasal spray?
1 answer
Further Information
Related Condition Support Groups
Related Drug Support Groups
Perskindol, Blue Ice, Halls Citrus Blend, 4Head, LOCABIOTAL, Gold Bond, Nostroline, Absorbine Jr Pain Patch, Mineral Ice, view more... Icy Hot Extra Strength, Icy Hot Patch, Valda Pastilles, Arjun, Blue Gel, Deep Cold Ice, Deep Freeze Cold Gel, Berri-Freez Spray, Medicated Foot, Halls Naturals, Halls Plus, N Ice, N Ice Cherry, N Ice Citrus, N Ice Eucalyptus, N Ice Honey / Lemon, ActivOn Arthritis, Cold & Hot, Stopain, ActivOn Joint & Muscle, Stopain Extra Strength, Meggezones, Gel Glace / Ice Cold, BENGAY Pain Relieving, Happinose, Koldets Cough Drops, Salonsip Aqua-Patch, Duro-Tuss Cough Lozenges, Vicks Cough Drop, Flexall, Universel Gel Extra-Fort, Universel Gel Regulier, Stopain Cold, Sucrets Herbal, Sucrets Ice
Menthol Patient Information at Drugs.com
Ask a Question
Top Group Members
In this group:
1 answer


text:
Loma Linda University
Loma Linda University Medical Center
Enrollment Information
Call us at: 909-558-1000
About LLU
Schools & Programs
Spiritual Life
Faculty Listing A-Z
Search by Name
List by School or Department
Leonard Bailey
LLU Teaching
Research & Grantsmanship
Patient Care
Honors and Awards
Faculty Directory
Leonard Bailey, MD
Distinguished Prof
, Cardiovascular & Thoracic Surg
School of Medicine
Distinguished Prof
, Pediatrics
School of Medicine
Research Presentations -- International
"The Voyage of Neonatal and Infant Heart Transplant." 19th Scientific Session of the Saudi Heart Association. Riyadh, Saudi Arabia ( 2/2008 )
"Autologous Extracardiac Lateral Tunnel Fontan." 19th Scientific Session of the Saudi Heart Association. Riyadh, Saudi Arabia ( 2/2008 )
Research Presentations -- National
Bailey L. "Past, Present, and Future of Neonatal Heart Transplantation."
University of Colorado, Denver Children's Hospital
. Denver, CO ( 2/2007 )
Bailey LL. "Past, Present, and Future of Pediatric Heart Transplantation."
The Second International Conference on Heart Failure in Children and Young Adults: New Strategies for Diagnosis and Treatment of Heart Failure
. Dana Point, CA ( 11/2006 )
Bailey L, Bailey N. "20 Years of Neonatal Heart Transplantation."
Western Section, American Urological Association
. Maui, HI ( 10/2006 )
Bailey LL. "Neonatal Heart Transplantation: Past, Present, Future."
Grand Rounds, Hospital for Sick Children
. Toronto, Canada ( 6/2006 )
Bailey LL. "Past, Present, and Future of Infant Heart Transplantation."
Grand Rounds, Texas Children's Hospital
. Houston, TX ( 5/2006 )
Bailey LL. "Pioneers in Transplantation Lecture Series: 20 Years of Neonatal Heart Transplantation."
ISHLT Annual Meeting and Scientific Sessions
. Madrid, Spain ( 4/2006 )
Bailey LL. "Hypoplastic Left Heart Syndrome: Contemporary Options."
American College of Cardiology 55th Annual Scientific Session
. Atlanta, GA ( 3/2006 )
Bailey LL. "History of Infant Heart Transplantation."
Department of Surgery Grand Rounds, Oregon Health Sciences University School of Medicine
. Portland, OR ( 10/2005 )
Bailey LL. "Pediatric Heart Transplantation: Current State of the Art."
2nd International Cardiovascular Disease Course: New Horizons in Cardiovascular Therapy
. Lima, Peru ( 8/2005 )
Bailey LL. "Pediatric Heart Transplantation: Current Status."
Congenital Heart Disease: Which Future?
. Palermo, Italy ( 5/2005 )
Bailey LL. "The Baby Fae Xenoheart Transplant: Audacious Origins, Auspicious Legacy."
Congenital Heart Disease: Which Future?
. Palermo, Italy ( 5/2005 )
Bailey LL. "Pediatric Heart Transplantation: Current Status."
Anthony R. C. Dobell Visiting Professor for Congenital Heart Surgery
. McGill University, Montreal, Quebec, Canada ( 11/2004 )
Bailey LL. "Quest for Cardiac Xenotransplantation."
McGill University CVT Grand Rounds
. Montreal, Quebec, Canada ( 11/2004 )
Bailey LL. "Pediatric Heart Transplantation: The Baby Fae Legacy."
Johns Hopkins Grand Rounds
. Baltimore, MD ( 9/2004 )
Bailey LL. "Pediatric Heart Transplantation - State of the Art."
17th Annual Meeting of the World Society for Cardio-Thoracic Surgeons
. Yokohama, Japan ( 7/2004 )
Presentations given to non-academic audiences
"REALLY LIVING Television Show." Adventist Media Productions. Simi Valley, CA ( 4/2008 )
Bailey LL. "Baby Fae''s Legacy and Beyond." Heritage Society Luncheon. Loma Linda, CA ( 11/2007 )
Bailey LL. "20 Years of Neonatal Heart Transplantation."
Big Hearts for Little Hearts Desert Guild
. Indian Wells, California ( 2/2007 )
© 2015 Loma Linda University 909-558-1000 |
Parking, Maps & Directions
Contact us
Loma Linda University Health
Medical Center
Site Index
Support Loma Linda
Media Resources
Spiritual Life
Privacy Information
Website Feedback


text:
YMMV: One Piece Film: Strong World
- Big Lipped Alligator Moment: Shiki's dance number.
- Moe: Xiao◊.
- Praising Shows You Don't Watch: The One Piece wiki made a poll for the best OP movie, and Strong World won by a landslide. Fair enough. The problem is, the poll was created before anyone had subbed the movie. Oda's involvement and its canon status automatically made it the best One Piece movie, regardless of how the film actually turned out. Granted, some fans might have seen and enjoyed the movie before it was actually subbed, but this doesn't invalidate the trope.


text:
An exclusive location with the magic of live music.
Between Piazza di Spagna and Piazza del Popolo, the Piano Bar Valadier is the ideal place for the events that are close to your heart. An exclusive location where the elegant and distinguished décor are combined with an intimate and relaxing atmosphere. Candlelight adds even more charm to the rooms, which are decorated with precious marbles, boiserie and works of art, and the furniture, upholstered with velvets and other fine fabrics. But what really makes the evenings special is the magic of the live music; around the piano, evenings come alive and enjoyment is guaranteed for those who let themselves go and sing, play and dance. The place is suitable for all types of events; during the day it is ideal for a welcome drink or coffee-break, in the evenings aperitifs and cocktails with a buffet. The whole place or individual spaces can be booked.
Virtual tour
Request information
- 1
- 2
- 3
City Tax in 2014
As formalized by the Council of Rome we inform our customers that from the 1st of September 2014 the city tax will be of € 6,00 per day per person for...
Special Offer Brillo Parlante
Give to the waiter a ticket for a movie, a live show, an exhibition etc. that you have used the same day, you will immediately receive a 15% discount off...
Download our presentation
Groupe Valadier is an important player in the hospitality sector.


text:
September 18, 2012
We all need to read this bible verse
Thanks for helping us associate the correct Pornstars to this video!
Help Us: Do these Pornstars appear in this video?
Pornstar Name:
All Comments (10)
We all need to read this bible verse
Love pussy
hey girl go pee for me
I want a threesome. I've pissed my pants and come watching it
mmmm really nice
welche geile votze möchte mich anpissen...meld dich bei mir


text:
Simmons ® Master SeriesTM 3-9 x 50 mm ProSportTM TruplexTM Reticle Scope! Be right on target with every shot.
The future of Riflescopes and Shotgun Scopes is here today! Featuring Simmons'® patented TrueZero adjustment system and QTA eyepiece with plenty of eye relief, the ProSport delivers the performance you need to ensure success each time you pull the trigger!
Simmons SureGrip rubber surfaces make the ProSport one of the easiest scopes to adjust under any shooting conditions.And, my friend, you can buy it at a reasonable Guide price and of course, my 100% Satisfaction Guarantee is included with everything you buy from me here at the Guide!
Quit squinting and missing... order your Scope today!


text:
Hanford, Calif., is setting up Accela Civic Voice, a cloud-based solution that could help its 55,000-plus residents become much more engaged.
Denver, Detroit, Memphis and Rochester, N.Y., will get technology consulting services as winners of the IBM Smarter Cities Challenge.
The Police Data Initiative aims to accelerate the development of data transparency and analysis tools to build trust between local police and the communities they serve and improve internal accountability.
Chicago is implementing a Bus Transit Management System to address the "bunching" that has contributed to inefficiency in its public transportation.
Adding graphics acceleration to a Texas town's virtual desktop system gave users performance equal to or better than their existing computers, accessible from almost any location.
An app alerts drivers sitting at red lights in Walnut Creek, Calif., that the light is about to change and refocuses them on the road.
Facing recruitment, retention and retirement hurdles, states are finding creative solutions to attract and keep cybersecurity talent.
The District of Columbia has partnered with BuildingIQ to use its cloud-based based heating, ventilation and air conditioning management solution in eight government buildings.
SceneDoc's mobile documentation platform lets police securely collect text, photos, video and audio in near real time, cutting multiple steps from the department's workflow.
Inrix's new services will help transportation officials better understand GPS-data driven traffic patterns.
Know of a great IT project and team that deserve some time in the spotlight? Involved in a project yourself that delivered great value or made a big difference for its users? Nominate those projects for a GCN Award. Here's how.
A business process management solution originally purchased for permitting is being put to much broader use.
A look at which state websites are falling short on Google's new mobile-friendly rankings and why.
The New Hampshire Department of Motor Vehicles is nearing the launch date for its Microsoft's Dynamics CRM-based Motor Vehicle Enterprise System.
With Google's PageRank putting new emphasis on mobile friendliness, agencies will feel more pressure to speed the move to mobile.
Advanced Search
8609 Westwood Center Drive, Suite 500
Vienna, VA 22182-2215
© 2015 1105 Media, Inc.
8609 Westwood Center Drive, Suite 500
Vienna, VA 22182-2215703-876-5100 © 1996-2014 1105 Media, Inc. All Rights Reserved.


text:
Re: [thewire] a Full Xmas Monty, anyone?
Expand Messages
- Jess Harvell wrote:
>OK, so I'm a day behind on this list (flamewars elsewhere to laugh at) but ...
> I also remember a Butthole Surfer's Xmas 7" (pitched somewhere between their
> gloriously stupid peak and their poretentiously stupid nadir) which, while I
> don't remember being particularly good, was a lot of fun to drop
> unsuspectingly admist the family Xmas morning or eve revelry...
*nobody* has mentioned Culturecide's "Depressed Chrismas"? Jeez, what a bunch a
happywarts ...
Jim Flannery newgrange@...
"There are sounds which seem to pass through all the protective gates
in the ear and reach into some nerve where the eschatology is stored."
-- Norman Mailer
np: Shadow Sampler 2000
nr: Arturo Perez-Reverte, _The Club Dumas_
Your message has been successfully submitted and would be delivered to recipients shortly.


text:
The cell modules may contain two or four cell units, called double and quad modules respectively. A quad module is shown in Figure 4. The module consists of external walls on all sides and a roof slab cast monolithically. Openings for cell doors, embeds and amenities are set at the time of casting. The exterior walls exposed to the weather are insulated. Electrical ducts, plumbing and other mechanical ducts are embedded in the concrete and placed through the triangular chases shared between two adjacent cells. Bunks, toilets and other amenities are fixed to the cells with tamper-proof bolts.
Download PDF
QuadMold Footprint
Precast Wind Tower Foundations embody a post-tensioned design that ensures structural integrity with the advantage of using modular precast concrete components. This allows for ease of manufacturing, delivery, and installation.


text:
Page 1
The Autographa californica M Nucleopolyhedrovirus ac79 Gene
Encodes an Early Gene Product with Structural Similarities to UvrC
and Intron-Encoded Endonucleases That Is Required for Efficient
Budded Virus Production
Wenbi Wu and A. Lorena Passarelli
Molecular, Cellular, and Developmental Biology Program, Division of Biology, Kansas State University, Manhattan, Kansas, USA
The Autographa californica M nucleopolyhedrovirus (AcMNPV) orf79 (ac79) gene is a conserved gene in baculoviruses and
cleusofinfectedcellsatlatetimes,suggestingacellularcompartment-specificfunction.Toinvestigateitsfunction,an ac79-
knockout bacmid was generated through homologous recombination in Escherichia coli. Titration assays showed that budded
virus(BV)productionwasreducedinthe ac79-knockoutviruscomparedtocontrolviruses,followingeithervirusinfectionor
thetransfectionofbacmidDNA.The ac79-knockoutvirus-infectedcellsproducedplaquessmallerthanthoseinfectedwithcon-
mationofocclusionbodiesin ac79-knockoutandcontrolviralDNA-transfectedcells,indicatingprogressionintothelateand
agivenquantityofinfectiousvirionssuggestedthatthe ac79-knockoutvirusproducedmorenoninfectiousBVininfectedcells
thanthecontrolvirus.Thestructureofthe ac79-knockoutBVdeterminedbytransmissionelectronmicroscopyappearedtobe
ofac79-knockoutvirus-infectedcells.Tubularstructureswerenotobservedfor ac79viruseswithmutationsinconservedendo-
viruses include four genera: Alphabaculovirus, Betabaculovirus,
Gammabaculovirus, and Deltabaculovirus (13). Alphabaculovi-
ruses and betabaculoviruses infect larvae of Lepidoptera; while
gammabaculoviruses and deltabaculoviruses, infect larvae of
Hymenoptera and Diptera, respectively (13).
During alphabaculovirus replication, two types of virions are
(ODV). The BV is produced as the nucleocapsid buds out from
the plasma membrane of the infected cell. BVs are essential for
establishing systemic infection in an infected insect by transmit-
ting infection from cell to cell. The ODV is formed in the nucleus
of the infected cell prior to being embedded within a crystalline
infection from larvae to larvae when a dead host releases ODV-
containing polyhedra into the environment and polyhedra are
ingested by another host.
The Autographa californica M nucleopolyhedrovirus (AcMNPV)
open reading frame (ORF) orf79 (ac79) has not been studied in
detail, and our knowledge of the function of ac79 stems from
genomic or proteomic analyses. The genomic sequence of ac79
ular mass of 12.2 kDa (2). Homologs of ac79 are found in baculo-
viruses of the genera Alphabaculovirus and Betabaculovirus, asco-
viruses, iridoviruses, and bacteria (23). A previous proteomic
concluded that Ac79 was associated with ODVs (5). It was sug-
gested that Ac79 is a member of the DNA repair UvrC endonu-
aculoviruses have circular double-stranded DNA genomes of
between 80 and 180 kbp (12) and infect arthropods. Baculo-
clease superfamily with similarities to intron-encoded endonu-
cleases, since it is predicted to contain two tyrosines spaced by
about 10 amino acids, the hallmark sequence RX3[YH], and a key
glutamate downstream of this sequence (1).
In this study, we characterized ac79 by mapping the transcrip-
the role of Ac79 during AcMNPV replication, we generated an
ac79-knockout virus, Ac79KO-PG, through homologous recom-
bination in Escherichia coli. The deletion of ac79 resulted in a de-
crease in BV production but did not affect viral DNA replication
or late and very late protein accumulation. Ac79KO-PG was able
to produce BV, but more virions than the control virus were not
infectious. Electron micrographs did not reveal virion structure
defects in the absence of ac79. Elongated tubular structures con-
taining the major capsid protein VP39 were produced in ac79-
knockout virus-infected cells. These tubular structures were not
observed for viruses carrying ac79 mutations in the UvrC/intron-
encoded endonuclease conserved residues.
Received 8 September 2011 Accepted 1 March 2012
Published ahead of print 14 March 2012
Address correspondence to A. Lorena Passarelli, firstname.lastname@example.com.
This article is contribution 11-401-J from the Kansas Agricultural Experiment
Copyright © 2012, American Society for Microbiology. All Rights Reserved.
jvi.asm.org 0022-538X/12/$12.00 Journal of Virologyp. 5614–5625
Page 2
Viruses, cell lines, and bacterial strains. Bacmid bMON14272 (Invitro-
gen), here referred to as AcBAC, containing an AcMNPV genome, was
propagated in E. coli BJ5183 cells as described previously (3). The virus
AcWT-PG was constructed by introducing the polyhedrin gene and the
enhanced green fluorescent protein gene (egfp) at the polyhedrin locus of
AcBAC (32). DH10B cells with helper plasmid pMON7124, encoding a
transposase, were purchased from Invitrogen. The Sf9 insect cell line,
27°C in TC-100 medium (Invitrogen) supplemented with 10% fetal bo-
vine serum, penicillin G (60 ?g/ml), streptomycin sulfate (200 ?g/ml),
and amphotericin B (0.5 ?g/ml).
sites for ac79, RNA was extracted from AcWT-PG-infected Sf9 cells and
collected at 6, 12, and 24 h postinfection (p.i.) by use of TRIzol reagent
measuring the optical density at 260 nm. Rapid amplification of 5= cDNA
ends (5= RACE) was performed by using a 5= RACE kit, version 2.0, ac-
cording to the handbook provided by the manufacturer (Invitrogen).
ac79-specific primer Ac79SP1 (5=-GGTTTGGCTTTGATGAGACGC-3=)
was used to synthesize first-strand cDNA. A nested ac79-specific primer,
Ac79SP2 (5=-GCTGTGATACACGAGCCGTA-3=), and the provided
strand cDNA as a template. PCR products were gel purified with the Gel
Extraction kit (Qiagen) and cloned into pCRII (Invitrogen) prior to de-
riving the nucleotide sequence. A total of five clones per time point were
sequenced in each of two independent experiments.
Time course analysis of Ac79 expression. A monolayer of Sf9 cells
(1 ? 106cells) was infected at a multiplicity of infection (MOI) of 5 with
locus (see below). The protein synthesis inhibitor cycloheximide and the
DNA synthesis inhibitor aphidicolin were added, and cell samples were
collected as previously described (32). Proteins were analyzed by sodium
dodecyl sulfate (SDS)–15% polyacrylamide gel electrophoresis (PAGE)
followed by immunoblotting or stored at ?20°C until further use.
Cell fractionation into cytoplasmic and nuclear fractions. Sf9 cells
(1 ? 106cells) were infected with Ac79HARep-PG at an MOI of 5 and
collected at 6, 12, 24, 48, and 72 h p.i. To fractionate the cells into cyto-
plasmic and nuclear fractions, the cell pellet was resuspended in 50 ?l of
dithiothreitol, 0.5% [vol/vol] NP-40) and kept on ice for 5 min. The cells
were disrupted and nuclei were released by using a prechilled Dounce
homogenizer (10 strokes with a tight pestle). Cells were centrifuged at
1,000 ? g for 5 min, and the supernatant was retained as the cytoplas-
mic fraction. Pelleted nuclei were washed five times with buffer A and
resuspended in 50 ?l of buffer A. Fractions were stored at ?20°C until
further use.
Immunoblotting. Immunoblotting was performed as previously de-
scribed (32). The primary antibodies used in these study were (i) mouse
monoclonal anti- hemagglutinin (HA) antibody (Covance), to detect
clonal anti-GP64 antibody (eBioscience), to detect the viral fusion envelope
protein; (iv) mouse monoclonal anti-IE-1 antibody (a gift from Linda Gua-
dotsugata MNPV (OpMNPV) polyhedrin antiserum (a gift from George
Generation of the ac79-knockout bacmid. The ac79-knockout bac-
mid was generated through homologous recombination in E. coli as pre-
the chloramphenicol resistance gene (Cm). A 1,178-bp PCR fragment,
containing a 1,038-bp Cm cassette and 70 bp of ac79 flanking regions at
each end, was amplified by using pCMR (29) as the template and primers
Ac79D1 (5=-CTGCGGCAAGACAATGGAAAATTGTACACGGGCATC
TTCGAATAAA-3=) and Ac79D2 (5=-TTATGCAACAAAAGTGGTTTG
AAGATTAAACCAGCAATAGACAAA-3=). Purified PCR fragments (1
?g) were electroporated into electrocompetent BJ5183 cells, and the re-
combinant ac79-knockout bacmid (Ac79KO) was selected, as previously
described (32), and confirmed by PCR amplification of inserted and
flanking DNA.
replacement of ac79 by Cm in the ac79 locus of AcBAC. Primers Cm5
(5=-CTTCGAATAAATACCTGTGA-3=) and Cm3 (5=-AACCAGCAATA
GACATAAGC-3=) were used to detect the correct insertion of the Cm
region of recombination, were used to confirm the expected deleted region
and the insertion of Cm at the ac79 locus. Primer pairs Ac7951/Cm3 and
Construction of the ac79-knockout and repair viruses and AcBAC
carrying the egfp and polyhedrin genes. To facilitate the examination of
virus-infected cells and to determine if the deletion of ac79 had any effect
into the polyhedrin locus of AcBAC by site-specific transposition, as
previously described (32). To this end, several donor plasmids were
constructed. Two primers, Ac7952(5=-GAGCTCATCTGCGTCTGCCA
ACATAT-3= [the SacI restriction site is underlined]) and Ac7933 (5=-TC
derlined]), were designed to amplify an 816-bp fragment containing the
native ac79 promoter (300 bp upstream of the ac79 ATG) and the ac79
ORF (Ac79POA), using AcBAC DNA as a template. This PCR product
was ligated into pCRII, and the resulting plasmid, pCRII-Ac79POA, was
confirmed by nucleotide sequencing. pCRII-Ac79POA was digested with
SacI and XbaI, and the Ac79POA fragment was inserted into pFB1-PH-
ACGTCGTATGGGTACAACTTATTTGCTAACAGGA-3= [the XbaI re-
striction site is underlined]) were used to amplify a 654-bp fragment that
contained the ac79 native promoter and the ac79 ORF with an HA tag
prior to the stop codon (Ac79POHA). The PCR product was ligated into
pCRII and confirmed by nucleotide sequencing. The resulting plasmid,
pCRII-Ac79POHA, was digested with SacI and XbaI to obtain the
Ac79POHA fragment and inserted into pFB-PG-pA (32), generating the
taining the helper plasmid pMON7124 and Ac79KO were transformed
with donor plasmid pFB1-PH-GFP, pFB-PG-Ac79POA, or pFB-PG-
Ac79POHA to generate the ac79-knockout virus Ac79KO-PG and two
repair viruses, Ac79Rep-PG with ac79 and Ac79HARep-PG with an HA-
genes, was described previously (32). All bacmids were cured of helper
plasmids as described previously (32). Bacmid DNA was extracted and
Construction of bacmids Ac79Y24AG26A-PG, Ac79R34K-PG, and
Ac79E72D-PG. To determine whether conserved amino acids in Ac79
were important for function, we changed the nucleotide sequences of
tyrosine and glycine at positions 24 and 26, respectively, to make
Ac79Y24AG26A-PG; arginine at position 34 to encode lysine and con-
struct Ac79R34K-PG; or glutamic acid at position 72 to encode aspar-
tic acid and construct Ac79E72D-PG. Three primer pairs were used
to construct donor plasmids pFB-PG-Ac79Y24AG26A, pFB-PG-
Ac79R34K, and pFB-PG-Ac79E72D for transposition by using a
QuikChange XL site-directed mutagenesis kit (Agilent Technologies)
and pFB-PG-Ac79POHA as the template. Primers Ac79 GIYmut f (5=-
CCAATTTTCCATTGTCTTG-3=) were designed to introduce mutations
into Ac79Y24AG26A-PG, primers Ac79 RtoKmut f (5=-CACGGGCATC
Efficient Infectious BV Production Requires Ac79
May 2012 Volume 86 Number 10 jvi.asm.org 5615
Page 3
ACGAGCAATCTTAACAGAAAGATAAAACAGCATTCGAAC-3=) and
Ac79 RtoKmut r (5=-GTTCGAATGCTGTTTTATCTTTCTGTTAAGAT
TGCTCGTGATGCCCGTG-3=) were designed to introduce a mutation
into Ac79R34K-PG, and primers Ac79 EtoDmut f (5=-GCCGCCCGCAT
GGATTACAATCTTAAGCGTAAATGC-3=) and Ac79 EtoDmut r (5=-G
CATTTACGCTTAAGATTGTAATCCATGCGGGCGGC-3=) were de-
signed to introduce a mutation into Ac79E72D-PG. Electrocompetent
DH10B cells containing helper plasmid pMON7124 and Ac79KO were
transformed with donor plasmid pFB-PG-Ac79Y24AG26A, pFB-PG-
Ac79R34K, or pFB-PG-Ac79E72D to generate Ac79Y24AG26A-PG,
Ac79R34K-PG, or Ac79E72D-PG, as described above.
Viral growth curve analyses and plaque assays. Sf9 cells (1.0 ? 106
cells/35-mm-diameter dish) were transfected with 1 ?g of bacmid DNA
using Grace's unsupplemented medium (Invitrogen) and Lipofectin (6),
The supernatants of transfected or infected cells were collected at various
time points to determine titers by 50% tissue culture infective dose
(TCID50) endpoint dilution assays (22) using Sf9 cells. Time zero was
defined as the time when the DNA-Lipofectin mixture or virus was re-
placed with fresh medium.
Sf9 cells were infected with AcWT-PG or Ac79KO-PG at an MOI of
0.01, 0.005, or 0.001, and plaque assays were performed as previously
fluorescence microscope using an eyepiece micrometer. The results were
analyzed by using GraphPad Prism, version 5.01 (GraphPad Software,
Comparison of AcWT-PG and Ac79KO-PG BV genome copy num-
bers and structural proteins. To compare the numbers of BV genome
copies of Ac79KO-PG and AcWT-PG, the same number of infectious BV
units (based on TCID50, 5 ? 107PFU) of each virus was concentrated by
centrifugation, and genomic DNA was purified as previously described
5 ?l of DNA was used as the template for quantitative PCR (Q-PCR) as
previously described (32).
The same number of infectious Ac79KO-PG or AcWT-PG units (5 ?
anti-GP64 antibody or anti-VP39 antiserum.
Viral DNA replication analysis. Sf9 cells (1.0 ? 106cells/35-mm-
diameter dish) were infected in triplicate with Ac79KO-PG or
AcWT-PG at an MOI of 5 and collected at different time points. Total
DNA was prepared, and Q-PCR was used to determine viral DNA
replication in virus-infected cells as previously described (32). The
results were analyzed by using GraphPad Prism, version 5.01 (Graph-
Pad Software, Inc.).
effect of ac79 on viral protein expression, Sf9 cells were infected with
A79KO-PG or AcWT-PG at an MOI of 5. At different time points, cells
were collected, and samples were prepared for immunoblotting as
described above. VP39 antiserum and OpMNPV polyhedrin antise-
rum, which cross-reacts with AcMNPV polyhedrin, were used as pri-
mary antibodies to immunodetect representative late and very late
Purification of BV and ODV. Sf9 cells were infected with
and the supernatant were separated by centrifugation. BV was concen-
third-instar Trichoplusia ni larvae were infected by the contamination of
an artificial diet with Ac79HARep-PG polyhedra, which were prepared
from Ac79HARep-PG-infected Sf9 cells. ODVs were purified as previ-
ously described (30).
Purification of BV and negative staining for transmission electron
microscopy. Sf9 cells were infected with Ac79KO-PG or AcWT-PG at
an MOI of 0.01. The supernatant of infected cells containing BV was
collected at 120 h p.i. The purification of BV was carried out as previ-
ously described (22). For negative staining, an electron microscope copper
grid was placed over 10 ?l of purified BV to absorb virions. Grid-bound
virions were stained with 10 ?l of a 2% uranyl acetate solution in water.
Stained virions were observed by using a Philips CM 100 transmission elec-
Electron microscopy and immunoelectron microscopy. Sf9 cells
(1.0 ? 106cells/35-mm-diameter dish) were infected with the indicated
virus at an MOI of 5. Infected cells were collected at 72 h p.i., fixed,
dehydrated, embedded, sectioned, and stained as described previously
(17). Samples were viewed as described above.
For immunoelectron microscopy, cells were embedded in LR White
resin (Ted Pella, Inc.), according to the technical notes provided by the
manufacturer. VP39 in Ac79KO-PG-infected cells was detected with rab-
bit polyclonal VP39 antisera, and HA-tagged Ac79 was detected with
FIG1 5=RACEanalysisofac79transcription.(A)ac795=RACEproductsat6,12,and24hp.i.M,DNAstandards.TwoDNAstandardsizesareindicatedatthe
The Ac79SP2 primer used for 5= RACE analysis is underlined. The asterisks and triangles indicate products from 5= RACE corresponding to the RACE products
in panel A.
Wu and Passarelli
jvi.asm.org Journal of Virology
Page 4
anti-HA antibody in Ac79HARep-PG-infected cells. These primary anti-
bodies and goat anti-rabbit immunoglobulin G 10-nm gold-conjugated
ing the primary antibody were used as background labeling controls. Im-
ages were obtained with a Philips CM 100 transmission electron micro-
scope at an accelerating voltage of 100 kV.
Mapping of the ac79 transcription start sites. The ac79 tran-
scription initiation sites were determined by 5= RACE analyses
using total RNAs isolated at 6, 12, and 24 h p.i. from AcWT-
PG-infected cells. 5= RACE products (Fig. 1A) obtained from
RNAs harvested at each time point were cloned, sequenced,
and mapped to four transcription start sites (Fig. 1B, asterisks).
At 6 and 12 h p.i., we detected 5= RACE products that mapped
to 55, 166, 167, and 168 nucleotides (nt) upstream of the pre-
dicted Ac79 translational start site (Fig. 1B). These sites were
not reminiscent of early or late start sites (i.e., TATA box, ini-
tiator sequence, or TAAG). The larger 5= RACE product ob-
FIG2 Ac79expressionandlocalization.(A)CellsweremockinfectedorinfectedwithAc79HARep-PGatanMOIof5.Atdifferenttimesp.i.,asindicated,
cells were harvested, and total cellular proteins were resolved by SDS-PAGE and detected with anti-HA antibody. mi, mock-infected cells. The numbers
to the left indicate the molecular masses of protein standards. The arrow indicates the expected migration for Ac79. (B) Cells were infected with
Ac79HARep-PG in the presence (?) or absence (?) of the DNA synthesis inhibitor aphidicolin or the protein synthesis inhibitor cycloheximide. Total
cellular proteins were immunoblotted using anti-HA antibody to detect Ac79 or anti-IE-1 antibody to detect IE-1. (C) Cells were infected with
Ac79HARep-PG at an MOI of 5, and cytoplasm and nuclear fractions were prepared at the indicated times p.i. Fractionated proteins were immunoblotted
using anti-HA, anti-GP64, or anti-IE1 antibody to detect Ac79, GP64, or IE-1, respectively. (D) BV and ODV were purified, and proteins were detected
by immunoblotting with anti-HA antibody. Ac92HARep-PG BV was used as a positive control. Anti-IE-1 antibody was used to detect the BV- and
ODV-associated IE-1. (E) Images of a cross section of Ac79HARep-PG-infected cells prepared at 72 h p.i., showing Ac79 by using an immunogold-
conjugated anti-HA antibody. Bars, 500 nm.
Efficient Infectious BV Production Requires Ac79
May 2012 Volume 86 Number 10 jvi.asm.org 5617
Page 5
served at 24 h p.i. (Fig. 1A, triangle) maps to 18 nt upstream of
the gp41 translation initiation codon and corresponds to the
gp41 transcription start site (Fig. 1B). gp41 is present upstream
of ac79 and is transcribed in the same direction.
Synthesis and subcellular localization of Ac79. Sf9 cells were
infected with Ac79HARep-PG, a bacmid carrying an HA-tagged
immunoreactive band of approximately 16 kDa was detected at 6
h p.i., and detection continued through 96 h p.i. (Fig. 2A). The
hibitor cycloheximide were individually added to cells to assess if
Ac79 synthesis required prior DNA replication. The synthesis of
Ac79 was reduced but was detected in aphidicolin-treated cells;
however, as expected, it was nearly abolished in the presence of
cycloheximide (Fig. 2B), suggesting that late genes or DNA repli-
cation was not necessary for Ac79 synthesis. As a positive control,
the same membrane was stripped from antibodies and reprobed
with anti-IE-1 antibody to detect an early viral protein (Fig. 2B).
To determine the subcellular localization of Ac79 during infec-
tion, Sf9 cells were infected with Ac79HARep-PG and collected at
plasmic and nuclear fractions, and proteins were detected following
at late and very late phases (Fig. 2C). To assess the fractionation effi-
We also examined whether Ac79 was associated with the BV
or ODV. BV or ODV was prepared from the supernatant of
Ac79HARep-PG-infected cells or whole larvae, respectively, and
HA-tagged Ac79 was detected by immunoblotting with anti-HA
to detect an HA-tagged protein. Ac79 was associated with neither
BV nor ODV (Fig. 2D). To verify the purification and loading of
protein. In addition, Ac79 did not specifically localize within
Ac79HARep-PG-infected cells (Fig. 2E).
of its construction by PCR. Ac79KO was constructed by deleting
the central portion of ac79 and retaining 115 nt from the 5= end
and 95 nt from the 3= end of the ac79 coding region to ensure the
expression of the neighboring genes ac78 and gp41 (ac80) (Fig.
AcMNPV [2]) was replaced with the Cm cassette (Fig. 3A).
The deletion of ac79 from the ac79 locus of AcBAC and the
insertion of the Cm cassette were confirmed by PCR (Fig. 3B).
Primers Ac7951 and Ac7931 (Fig. 3A) were used to confirm the
deletion of 105 bp within the ac79 coding region and its replace-
ment with the 1,038-bp Cm cassette. Primers pairs Ac7951/Cm3
and Cm5/Ac7931 (Fig. 3A) were used to confirm the recombina-
tion junctions upstream and downstream of ac79, respectively.
Primer pair Cm5/Cm3 was also used to confirm the insertion of
were as predicted following successful recombination (Fig. 3B).
FIG 3 Construction of recombinant bacmids. (A) Strategy for construction of Ac79KO. An ac79 fragment (105 nt) was deleted and replaced with the
chloramphenicol resistance gene (Cm) between nt 65385 and nt 65498 of the AcMNPV genome (2). (B) PCR confirmation of Ac79KO. The table below
the polyhedrin (polh) and enhanced green fluorescent protein (egfp) genes inserted into the polyhedrin locus by Tn7-mediated transposition. (D) Schematic
diagram of Ac79KO-PG, Ac79Rep-PG, and Ac79HARep-PG, showing polh and egfp inserted into the polyhedrin locus after Tn7-mediated transposition.
Wu and Passarelli
jvi.asm.orgJournal of Virology