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Mendelssohn Choir of Pittsburgh announces upcoming season
The Mendelssohn, the go-to chorus of the Pittsburgh Symphony Orchestra, announces its upcoming season, including a performance with the PSO at East Liberty Presbyterian Church in October. As Mary Ann Lapinski, the choir's executive director, wrote to me in an email:
"The Pittsburgh Symphony Orchestra is the Mendelssohn Choir's primary artistic partner. Usually this means that the Mendelssohn Choir that performs with the PSO as a 'guest artist.' What makes the October concert so thrilling for us, is that the tables are being turned; we are producing the concert and the PSO is performing for the Mendelssohn as its orchestra under Betsy's baton. This 'role reversal' speaks to the mutual respect that the PSO and MCP have for each other's artistic excellence and vision."
More from the press release:
The Mendelssohn Choir of Pittsburgh Announces Its 2014-2015 Season—
A Season of Earthly Delights and Celestial Bliss
The Mendelssohn Choir of Pittsburgh's 106th season entitled "Heaven and Earth" will take audience members on a journey from heavenly bliss to the dramatic depths of despair to sublime tranquility—along with a dash of earthly fun to round it all out!
Our season begins with a special collaboration with the Pittsburgh Symphony Orchestra when the Mendelssohn Choir (MCP) performs Faith & Fate: Beethoven's Mass in C and Brahms Schicksalslied (Oct. 5 at East Liberty Presbyterian Church). The Pittsburgh Symphony Orchestra, under the direction of Mendelssohn Choir Music Director Betsy Burleigh, will accompany MCP in this musical exploration of a kinder, gentler Beethoven and a blustery Brahms. "I am thrilled to be working directly with the fabulous musicians of the Pittsburgh Symphony," says Burleigh, "and this new collaboration shows the PSO's support of the Mendelssohn, opening the door for more great music making for the city of Pittsburgh." Next the Mendelssohn Choir will present Gioachino Rossini's Petite Messe Solennelle (March 22 at East Liberty Presbyterian Church). Called "the last of my sins of old age" by Rossini, this exquisite work for four soloists and chorus was Rossini's last major work and makes a fitting climax to the life of the "Italian Mozart," the man then considered the greatest opera composer of all time.
Then the season turns earthward for a delightful evening of song called I Got Gershwin! which will include excerpts from Porgy and Bess, and standards like "I Got Rhythm," "Embraceable You," "The Man I Love," "Let's Call The Whole Thing Off," and "Love Walked In" (May 2 & 3 at the Hillman Center for Performing Arts). Audiences will hear rarities from Hollywood, solo songs, and some of Gershwin's own piano arrangements and Preludes.
The Mendelssohn Choir will present its second annual Cabaret Fundraiser (Feb. 14 at Olive or Twist) called quite fittingly Let's Fall in Love! The Junior Mendelssohn Choir, under the direction of Maria Sensi Sellner, will perform its Fall Concert at Westminster Presbyterian Church (Nov. 9).
Tickets for the above concerts will go on sale August 1 at www.themendelssohn.org.
MCP performances with the Pittsburgh Symphony Orchestra in 2014-2015 include: Sci-Fi Spectacular with the PSO Pops Orchestra at Heinz Hall (Nov. 14-16); Music for the Spirit with the PSO, location TBA, (Nov. 20); Holiday Pops with the PSO at Heinz Hall (Dec. 12-21); and Beethoven's Ninth Symphony with the PSO at Heinz Hall (June 5-7). Tickets for these performances can be purchased at www.pittsburghsymphony.org.


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Phiyega must resign - FriedmanChantall Presence
Political analyst Steven Friedman says Riah Phiyega should quit and political parties welcome the findings and recommendations of the Marikana report.
Unisa reveals SABC chair Ellen Tshabalala's dismal marksChantall Presence
A parliamentary committee has heard that not only did Tshabalala fail to qualify, she didn't even score high enough to reregister for the course.
Investigation into Ellen Tshabalala's qualifications continuesChantall Presence
MPs felt vindicated after a court denied the SABC chair an order to halt investigations into allegedly false qualifications on her CV.
Nkandla: Opposition's corruption charges against Zuma centralisedChantall Presence
Opposition parties have charged President Jacob Zuma with corruption at various police stations, and the charges have been taken to a national level.
EFF threatens court action if MPs suspendedChantall Presence
The EFF has threatened court action should the National Assembly approve a recommendation to suspend 12 of its MPs without pay.
Malema, Shivambu thrown out of Parliament - againChantall Presence
The two EFF leaders have been thrown out of Parliament for a second time after calling Deputy President Cyril Ramaphosa a murderer.
EFF halts Parliamentary proceedingsChantall Presence
Julius Malema and his fellow MPs made good on their threat to block all motions in the House on Wednesday, causing proceedings to be suspended.
'We are going to shut down business' - MalemaChantall Presence
Julius Malema has threatened mass protest following the Gauteng Legislature's decision to bar EFF's members from wearing their red uniforms.
SA's 'sad story of housing' - Lindiwe SisuluChantall Presence
The housing delivery rate fell by 25% in the past five years and the backlog stands at 2.3-million, said Human Settlements Minister Lindiwe Sisulu.
Malema: EFF the rules of ParliamentChantall Presence
The EFF are in Parliament to pursue a revolution, not rules, Julius Malema said on Friday, as his party staged a protest outside the legislature.

subset: commoncrawl


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Live updates: Israeli-Palestinian violence
Inside the hidden world of hostage taking
20 Nov 2012 20:21 GMT | Sport, Cricket, India, United Kingdom
Media organisations called on Tuesday for the world cricket's governing body to intervene in a dispute over coverage restrictions of the Test series between India and England, saying the row was damaging the game.The News Media Coalition, writing on behalf of a group of news agencies, newspapers and publishers, urged the International Cricket Council (ICC) to "use its influence and global standing" to persuade the Indian cricket board (BCCI) to lift the curbs which have triggered a text and photo blackout of the series."We recognise that the ICC does not have direct authority of the media policy of individual national cricket bodies but view the ICC as the collective custodians of the good name of the sport, fair play and the promotion of the game," said the letter whose signatories include Agence France-Presse."The BCCI's policy of locking out an important sector of the news industry risks damaging much that the ICC has sought to achieve."
"The BCCI's policy of locking out an important sector of the news industry risks damaging much that the ICC has sought to achieve"
News Media Coalition letter
News agencies such as AFP, Thomson-Reuters and the Associated Press have all suspended text and photo coverage of the four Test series over the BCCI's decision to bar photo counterparts such as Getty Images and Action Images.English newspapers have also refused to publish live pictures either in their online or print editions, instead running file images or even spoof cartoons. The BCCI did offer a small number of their own pictures but these were rejected. "It surely does not help those who feel cricket should enjoy maximum visibility, even greater participation and acceptance within the staging of sporting events such as Olympics," said the letter to the ICC chief executive Dave Richardson and Colin Gibson, its head of media.The ICC has drawn up proposals for Twenty20 cricket to eventually become an Olympic sport.Kevan Gosper, chairman of the International Olympic Committee's press commission, is among those to have criticised the BCCI stance, calling it "a direct attack on the freedom of the media".The accreditation dispute is the latest between the BCCI and media organisations to mar the series.Satellite broadcaster Sky, which holds the British rights to the series, is having to commentate from its London headquarters off a live picture feed rather than pay a reported additional $795,000 to the BCCI.No reaction was immediately available from the BCCI.
Source: AFP
India's spinners inspired a 90-run win to heap misery on defending champions England in Group A at the World Twenty20.
Politics, Sport, Cricket, India, United Kingdom
A revitalised India subject England to another demoralising loss during their ODI series in New Delhi.
Sport, Cricket, United Kingdom
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Saved by MSF's Dignity I, refugees explain why they risked their lives on the treacherous route from Africa to Europe.
Refugees, Mediterranean, Human Rights
Global ride-sharing company Uber has faced growing legal pressure in Australia but continues to steer its course.
Business & Economy, Australia, Asia Pacific
UN investigators to determine if the government committed "grave violations" after disabled people ordered back to work.
Human Rights, Health, Mental health
Dutch safety team's final report into the downed Malaysian airliner will not place blame for the attack that killed 298.
MH17, War & Conflict, Ukraine
Israel's Netanyahu calls security cabinet meeting to discuss situation as three Israelis die in attacks by Palestinians.
Israeli–Palestinian conflict, Israel, Middle East
MH17, War & Conflict, Ukraine, Russia, Europe
Battling media narratives in Russian and US bombing campaigns; plus, the conundrum of covering India-Pakistan relations.
Media, Middle East, Syria
Follow the incredible journey of Asadullah Rahman, the youngest inmate to be held at Guantanamo Bay.
War & Conflict, Afghanistan, Taliban
People & Power investigates India's Hindu fundamentalists and their influence on the country's government.
India, Politics, Human Rights
101 East explores why over 300 Australian children are abducted by a parent and taken overseas every year.
Australia, Asia, Law


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Comment Ensure offline works (Score 2) 508
" I assign papers that must be typed, I have papers turned in online, and I plan to freely refer to texts, videos, and other resources that are available online."
Typed could be done with a typewriter. The onus is on YOU to scan if you need electrons. While it certainly is harder, having to think before typing can provide the student with some benefits as well!
Provide pointers to offline texts that should suffice (books, libraries, etc. still exist).
Videos and other resources can be viewed, when they have access at school, library, or tablet (Amazon is rumored to have a $50 one in the wings).
Since you aren't teaching at an elite private school, make sure that you aren't depriving your students of the chance to learn!


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Solgar introduces a new calcium and vitamin D3 supplement for children. Solgar U-Cubes Calcium with D3 contain 600 IU of vitamin D3 per serving and 500 mg of calcium per serving (four gummies). These nutrients support healthy bones and teeth.
Quinoa Ready to Eat Meals by Cucina & Amore require little preparation. They come in a durable, microwaveable package to allow you to easily heat and store your meal. These all-natural, vegan meals come in: Mango & Jalapeno, Basil Pesto, Spicy Jalapeno & Roasted Peppers, and Artichoke & Roasted Peppers.
Bandar Monkey Foods released Lentil Crisps. Inspired by poppadums, traditional street food enjoyed in India, these crisps serve as a good source of protein and have 50% less fat than potato chips. Original Cumin and Green Chili Garlic flavors are currently available.
Daily Greens expanded its line to include REPLENISH, a chocolate hemp milk. This dairy milk alternative is made with hemp seeds, wheatgrass, Madagascar vanilla, coconut nectar, camu camu and raw cocoa. It is made with only organic and all-natural ingredients.
New from Mamma Chia is the Organic Clean Energy line of drinks. Each variety contains 2,500 mg of omega-3s from chia seeds along with brewed guayusa tea. They deliver 90 mg of natural caffeine and 4 g of protein, and are an excellent source of fiber. Favors include Cherry Charge, Blackberry Blast, Grape Power and Raspberry Razz.
Ruby's Naturals added five new flavors to its Ruby Rocket's line of frozen pops: Celestial Cherry, Far-Out Fudge, Planetary Pink Lemonade, Meteorite Mango and Gravity Grape . These pops combine flavors that kids enjoy along with non-GMO vegetables and fruits and low amounts of sugar (four to six grams). Ingredients include sweet potato, cherry, butternut squash and acai. All five flavors are gluten-free, dairy-free, Kosher and vegan, with no artificial colors or flavors.
Cluck 'n Moo is making a new addition to its half-chicken, half-beef burgers with the Cluck 'n Moo Burger with Kale. This new product combines the healthful benefits of kale along with the same juiciness and flavor of the original Cluck 'n Moo burger. Cluck 'n Moo burgers are made with grass-fed beef and chicken, with no antibiotics or hormones.
Fishpeople offers a line of ready-to-eat entrees and soups featuring sustainable seafood from the Pacific Northwest. Options include Albacore Tuna in Yellow Coconut Curry, Chinook Salmon in a Chardonnay Reduction Sauce, and Creamy Razor Clam and Bacon Chowder. Consumers can trace the source via a code on the back of the packaging.
KeVita offers Cleansing Probiotic Tonics with apple cider vinegar (30 g). They have ingredients that promote cleansing. Each bottle includes probiotics (4 billion CfUs) and vitamin C. The four launch flavors are Kale Lemon, Red Beet, Turmeric Ginger and Cinnamon.
New York, NY—A study has found...
Elmwood Park, NJ—After an...
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Ask a Scientist Event @ the Northeast Library
October 1st, 2015 by hillaryu
Good, Simple Care Can Keep Your Skin Healthy for Years to Come
September 23rd, 2015 by hillaryu
As a dermatologist, I often hear from patients that they don't have time for intensive skin care. However, people should still take care of their skin by doing the basics over their lifetimes.
Good skin care and healthy lifestyle choices can help delay the natural aging process and prevent various skin problems.
Protect yourself from the sun
One of the most important ways to care for your skin is to protect it from the damaging effects of overexposure to the sun's harmful ultraviolet (UV) rays. A lifetime of sun exposure can cause wrinkles, age spots and other problems, like skin cancer.
For the most complete sun protection
- Use sunscreen – Use a broad-spectrum sunscreen with an SPF of at least 30. Apply it generously and reapply every two hours or more often if swimming or perspiring.
- Seek shade – Avoid the sun between 10 a.m. and 2 p.m., when the sun's rays are strongest.
- Wear protective clothing – Cover your skin with tightly woven, long-sleeved shirts, long pants and wide-brimmed hats. Also consider laundry additives, which give clothing an additional layer of UV protection for a certain number of washings.
Don't smoke
Smoking makes skin look older and contributes to wrinkles. It narrows the tiny blood vessels in the outermost layers of skin, which decreases blood flow. This depletes the skin of oxygen and nutrients important to skin health.
Smoking also damages collagen and elastin, the fibers that give skin strength and elasticity. In addition, the repetitive facial expressions people make when smoking can contribute to wrinkles.
If you smoke, the best way to protect your skin is to quit. Ask your doctor for tips or treatments to help you stop.
Daily cleansing and shaving can take a toll
To keep it gentle:
- Limit shower or bath time – Hot water and long showers or baths remove oils from skin. Limit shower or bath time and use warm, not hot, water.
- Avoid strong soaps – Strong soaps can strip oil from skin. Instead, choose mild cleansers.
- Shave carefully – To protect and lubricate skin, apply shaving cream, lotion or gel before shaving. Use a clean, sharp razor. Shave in the direction the hair grows, not against it.
- Pat dry – After washing or bathing, gently pat or blot your skin with a towel so some moisture remains on your skin.
- Moisturize dry skin – If your skin is dry, use a moisturizer that fits your skin type. For daily use, consider a moisturizer with SPF. Ointments and creams are better for dry skin since they are more effective at sealing in moisture. Lotions, which are often in pump bottles, have a high water content that tends to evaporate off of skin.
Anti-wrinkle creams
The effectiveness of anti-wrinkle creams depends, in part, on the active ingredient or ingredients. Common ingredients that may result in slight to modest improvement in the appearance of wrinkles, include:
- Retinol – Retinol is a vitamin A compound, the first antioxidant to be widely used in nonprescription wrinkle creams. Antioxidants neutralize free radicals – unstable oxygen molecules – that break down skin cells and cause wrinkles.
- Vitamin C – Vitamin C may help protect skin from sun damage. Before and between uses, wrinkle creams containing vitamin C must be stored to protect them from air and sunlight.
- Hydroxy acids or exfoliants – These substances remove the upper layer of old, dead skin and stimulate growth of smooth, evenly pigmented new skin.
- Coenzyme Q10 – This ingredient may help reduce fine wrinkles around eyes and protect skin from sun damage.
- Tea extracts – Green, black and oolong tea contain compounds with antioxidant and anti-inflammatory properties.
- Grape seed extract – In addition to its antioxidant and anti-inflammatory properties, grape seed extract also promotes wound healing.
- Niacinamide – This substance is related to Vitamin B-3 (niacin). It helps reduce water loss in the skin and may improve skin elasticity.
Healthy diet
A healthy diet can help people look and feel their best. Eat plenty of fruits, vegetables, whole grains and lean proteins. Although the association between diet and acne isn't clear, some research suggests a diet rich in vitamin C and low in unhealthy fats and processed or refined carbohydrates, might promote younger-looking skin.
Manage stress
Uncontrolled stress can make your skin more sensitive and trigger skin problems. To encourage healthy skin – and a healthy state of mind – take steps to manage stress. Set reasonable limits, scale back your to-do list and make time for things you enjoy.
Medical expertise
A dermatologist can help people create a personalized skin care plan by assessing your skin type, evaluating your skin's condition and recommending products likely to be effective. If you're looking for more dramatic results, a dermatologist can also recommend medical treatments for wrinkles, including prescription creams, Botox injections or skin-resurfacing techniques.
Catherine Pham, MD, is a board certified dermatologist who practices at Virginia Mason University Village Medical Center. For more information, visit the Virginia Mason website.
Sponsor (advertise with us)
ANNIE at the Paramount Theatre Through September 26
September 23rd, 2015 by hillaryu
KeyBank Broadway at The Paramount is proud to announce that the new U.S. National Tour of ANNIE will play Seattle's Paramount Theatre from September 20–26, 2015.
Directed by original lyricist and director Martin Charnin for the 19th time, this production of ANNIE is a brand new physical incarnation of the iconic Tony Award®-winning original. Tickets for the engagement are available on-line, by calling 877-STG-4TIX (784-4849) or in person at select Ticketmaster locations and at The Paramount Theatre Box Office (Monday through Friday, 10am to 6pm).
This brand new production features a 25 member company: in the title role of Annie is Issie Swickle, a 10-year-old actress from Davie, FL, making her tour debut. Gilgamesh Taggett will star as Oliver Warbucks. In the role of Miss Hannigan is Lynn Andrews. Also starring in the tour are Ashley Edler as Grace, Garrett Deagon as Rooster, Lucy Werner as Lily and Jeffrey B. Duncan as FDR. Sunny, a 5-year-old rescue terrier mix, stars as Sandy.
ANNIE will be at Seattle's Paramount Theatre for three more performances:
Thursday, September 24 7:30pm
Friday, September 25 8:00pm
Saturday, September 26 2:00pm – An ASL / AVIA Interpreted
I had the pleasure of attending last night's show and it was phenomenal. The sets, costumes and actors were spectacular and I enjoyed laughing with the audience around me. ANNIE is a classic and I left with several of the songs playing through my head for the rest of the evening. This is a great show for families!
See this one before it's gone!
Wedgwood Community Volunteers
September 14th, 2015 by hillaryu
(Photo courtesy of the Seattle Audubon)
Help Wedgwood and John Rogers students have FUN!
Finding Urban Nature (FUN) is Seattle Audubon's free environmental education program in Seattle Public Elementary Schools.
FUN is an environmental education program that introduces students to the natural world in their own schoolyard habitats through observation, discovery, and scientific inquiry.
Volunteers work with small groups of 4-5 students for 4, one-hour lessons, over the course of 4 weeks.
The program needs volunteers at Wedgwood and John Rogers Elementary Schools.
Please respond as soon as possible to be a part of FUN training in October. Contact Christine at 206-523-8243 ext. 19 if interested.
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Art Transforms Boarded Windows on Magnuson Park's Main Street
September 4th, 2015 by hillaryu
With funds from a Seattle Department of Neighborhoods grant, Sand Point Arts and Cultural Exchange (SPACE) and teaching artist Angela Larsen worked through a series of summer Saturdays with 11 teens from Solid Ground Housing to transform the boarded up windows of Building 18 into the cheerful and welcoming artworks now greeting park visitors.
The project goals were to beautify the vacant building on the main street of the park, and give the youth a chance to become familiar with different areas of the park and its buildings. "We wanted to make sure that kids living in the Solid Ground Housing of the park know they have the entire park as their community, not just the housing area where they live. We worked in several areas of Building 30 to produce the artworks and the kids enjoyed the chance to experience parts of the park unfamiliar to them, like the Officers Club and Workshop," says Julianna Ross, Executive Director of the non-profit arts organization.
SPACE had requested the artist and kids work with a nature theme so Larsen and the participants began the project with a walk in the park observing hues, texture and shape. From this, Larsen created a achievable motif inspired by Scandinavian pattern and design. The panels were installed by the Seattle Conservation Corps, with Seattle Parks and Recreation and Lowe's also offering generous assistance.
Building 18 recently underwent structural stabilization and is eligible to be redeveloped for public/private uses and add to the vitality of the park.
Sand Point Arts and Cultural Exchange funds, facilitates and promotes arts and cultural uses of Magnuson Park for the public and runs the Magnuson Park Gallery in Building 30 West, 7448 63rd Street NE, Seattle, WA 98115.
Learn more online.
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Cat adoption event – Meadowbrook Community Center
September 2nd, 2015 by hillaryu
Meadowbrook Community Center
4030 NE 109th St.
Seattle, WA 98125
September 12th
Noon – 3 pm
The Seattle Animal Shelter will host a cat adopt-a-thon on Saturday, September 12th, at the Meadowbrook Community Center. The event runs from noon to 3 p.m. and features numerous kittens and cats of different breeds. Cats available at this event have been living with foster parents, who are available at the event to share information about the personality and habits of the cats with potential adopters, helping to make a perfect match.
For quicker entrance to the event, you can complete our application before you arrive.
Adoption prices range from $45 to $135 (plus applicable license fees) and include:
- Initial vaccinations
- De-worming
- Feline Leukemia testing
- Certificate for free health exam at local veterinarians
- Spay or neuter
- Microchip
To learn more, visit the Seattle Animal Shelter's website.
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Save the Date: City Fruit Orchard & Harvest Tour
August 29th, 2015 by hillaryu
Seattle remains one of the few urban environments boasting an extensive variety of historic orchards and fruit trees. Today, hundreds of heirloom varieties continue to inspire homeowners, orchardists, community volunteers, and cidermakers.
On Sunday, October 4, visit Seattle's historic orchards throughout the city, including 7 orchards in North Seattle. Each orchard is planning special activities, including cider pressings, historical tours, and more! Your self-guided tour will take you throughout Seattle's neighborhoods to a hidden orchard, a former homestead, and other beautiful community sites.
Learn more about maintaining fruit trees in the Pacific Northwest or just enjoy the fall harvest season!
Tickets can be purchased online or at the following locations:
Roosevelt Whole Foods
City Fruit – El Centro de la Raza, 2524 16th Ave South, Room 301 (Monday through Thursday, 10am to 4pm)
City People's Garden Store – 2939 E Madison Street
Volunteers are also needed to help during the Orchard & Harvest Tour! If interested in helping out, contact Natalie Place.
$10 City Fruit Members/ $12 Non-Members/ $20 Family
Membership Month Special:
Purchase a City Fruit household membership at $50, receive free Orchard & Harvest Tour ticket
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Sustainable NESeattle end of Summer and Fall events
August 26th, 2015 by hillaryu
Upcoming in September:
Sept 19th is the Harvest Skills Fest.
This is the first of these skills events focusing on specific skills that help us make the most of our harvest. Come on out to learn and share these important and seasonally-appropriate skills. Volunteers are needed to instruct classes on things like dehydrating, seed saving, canning (pressure cooker or water bath), pickling, root cellaring, winter gardening, or perhaps you have a special preservation technique that you do and would like to share. If you would like to help, please email Joann with subject: Harvest Fest
Also coming in Sept–
September 15 is the next meeting of the Local Economy/Local Currency and LIONNES group, 7:30 - 9 at a location to be determined. Check out their website for more to find out more about local investing.
September 16 is the seventh annual Barter Fair and Cider Pressing, 1– 4pm, LOCATION: NE Seattle Tool Library parking lot.
Upcoming in October–
Monthly meetings return to the second Thursdays of the month, 7:00–9pm,
Check out their website for details on topics and locations.
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Wedgwood Community Council TREE SHIRT Contest
August 21st, 2015 by hillaryu
By Valerie (originally appeared on the Wedgwood Community Council website)
Become a local celebrity by designing the Wedgwood Community Council's next "tree-shirt," one of our most popular membership benefits! The design itself does not have to contain trees if you don't want it to, BUT the design should represent a connection to our community in some way.
Submissions will be accepted from July 25 to September 15, with the winner announced on October 1.
All entries that meet the following criteria will be posted here on this WCC page, where all current WCC members will be able to see and vote on the designs. The winning designer will not only see his or her design on all of our tree-shirts for the coming year, but will also receive a FREE tree-shirt for three family members.
You do not have to be a member of the WCC to enter the contest, but you must live within the Wedgwood neighborhood boundaries of NE 75th to 95th Streets (see map).
Tree-shirt specifics:
* Designs will be printed on unisex 100% cotton T-shirts; white or green backgrounds available – you decide!
* Designs should consist of two ink colors maximum; ink colors are of your choice, but please note limited colors are available which will work on the dark green background – white, gold and other pale colors.
* Maximum size of art: 12" wide by 11" high OR 11" wide by 14" high.
* Please submit high-resolution scans or Photoshop files only with at least 300 DPI or Vector art.
* Physical art accepted as well (we can scan your design.)
Please include the following information with all submissions:
* Name, phone number and email address
* Preferred shirt color: white or dark green background for your design.
* Preferred ink colors (general colors or PMS colors accepted)
Email your submission or any questions to: email@example.com
OR mail your submission to: PO Box 15770, Seattle, WA 98115
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Wedgwood/Chad Shanks Memorial Car Show
August 21st, 2015 by hillaryu
It's time again!
King Street Bar & Oven and The Wedgwood Broiler present the 8th Annual Wedgwood/Chad Shanks Memorial Car Show!
August 22nd, 2015
10:00 a.m. – 3:00 p.m.
Parking Lot of the Wedgwood Broiler
8230 35th Ave NE Seattle, WA. 98115
Lot opens at 9 am for vehicle staging.
$15 entry fee for all vehicles (includes 2 raffle tickets). Free admission for spectators.
All net proceeds will benefit the American Cancer Society.
All makes and models are welcome! Motorcycles too! This true neighborhood car show encourages people with all stages of vehicles to be a part of it from works in progress to beautiful classics to bonafide trailer queens. You will see it all here!
TROPHIES for various classes awarded, dash plaques for the first 30 vehicles and raffle prizes! 50/50 raffle also.
T-shirts available for purchase the day of show.
Come and spend a day in the sun with people who love their cars.
The Wedgwood Broiler's full menu will be available starting at 8 am.
Drink and beer specials will run all day.
Sponsored by King Street Bar and Oven, the Wedgwood Broiler, Fireball Whiskey, Bird Dog Whiskeys and Budweiser.
If you are interested in bringing a car, truck or motorcycle, please contact Troy at 206-852-8769
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|ISSN 1088-6826(online) ISSN 0002-9939(print)
The existence of a maximizing vector
Abstract | References | Similar Articles | Additional Information
Abstract: Let be a complex Lebesgue space with a unique duality map from to , the conjugate space of . Let be a compact operator on . This paper focuses on properties of and . We adapt an example due to Halmos to show that for , there is a compact operator on with the semi-open interval . So is not attained as a maximum. A corollary of the main result in this paper is that if , and , then is attained as a maximum.
Retrieve articles in Proceedings of the American Mathematical Society with MSC (1991): 47A12
Retrieve articles in all journals with MSC (1991): 47A12
Additional Information


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RSVP today!
Our next One IT gathering will be held on Wednesday, Oct. 21 and our focus is on YOU! Please join us from 3-5 p.m. at the Alumni House.
CalNet Login: More Than a New Look
The login screen for the campus CalNet system is getting a new look plus improved security on Tuesday, Oct. 20.
Join Us on Sept. 24
Campus faculty and researchers are invited to a Research IT Town Hall on Thursday, Sept. 24 to find out more about several campus services available to the UC Berkeley research community.
View Tools
As we kick off the fall semester, the bConnected team has made several enhancements to support increased communication and collaboration among UC Berkeley faculty, staff, and students.


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Ask Better Homes & Gardens
Experts and BHG readers answer.
Expertssee all experts
no cucumbers on vine
Are the cucumber vines blooming yet? Most varieties must be pollinated before they will form fruit. (There are a few exceptions, called parthenocarpic, or seedless cucumbers.) The first blossoms that form on the vine are all male. Eventually most varieties begin to alternate male and female blossoms. Only the female blossoms can develop into fruit. You can tell the difference by looking at the base of the blossom. Male blossoms will have a thin stalk supporting them. Female blossoms will have a swollen stalk that resembles a miniature cucumber. Again, there are exceptions, called gynoecious, or all-female cucumbers. Unless there is a male plant nearby, the all-female vines won't produce fruits. And because cross-pollination is involved, pollinating insects such as bees must be present. Or you can do the pollinating yourself with a cotton swab dabbed from a male flower to a female flower.


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Celeb News Pop: Legends of Summer Prove They Can Take the Heat
Jay Z and JT shared in embellishing and riffing on each other's material. When Justin Timberlake sang, several numbers into his Friday night concert with Jay Z at Yankee Stadium, "It feels like something's heating up," he wasn't just bringing sexy back. Jennifer Lawrence went from famous to super?-famous when she signed on to play Katniss Everdeen in The Hunger Games movies. Jen encouraged Shailene Woodley to take on the role of Tris in the upcoming film, "Divergent' mixed in with a lil career advice. "It will be the best thing that ever happened," Lawrence told her. "But don't be an idiot, do a sex tape, or do drugs. And go to Whole Foods when your movie opens." Another day, another Vanessa Hudgens bikini sighting. This time, the 24-year-old actress was spotted indulging in some R&R in Italy. On a boat. With a gold body chain. Hudgens, who's been promoting her new flick The Frozen Ground in Europe, showed off her bikini body in a black two-piece as she divided her afternoon between...Read more?


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How do you consider yourself as a certified Adamsonian?
Let me count the ways..
- When you don't feel like eating at school.. Adamsonians usually go to 106 more words
How do you consider yourself as a certified Adamsonian?
Let me count the ways..
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Page 1
Expression of multiple human endogenous retrovirus surface envelope proteins
in ovarian cancer
Feng Wang-Johanning1,2*, Jinsong Liu3, Kiera Rycaj1, Miao Huang1, Kate Tsai1, Daniel G. Rosen3, Dung-Tsa Chen4,
Danielle W. Lu5, Kirstin F. Barnhart1and Gary L. Johanning1
1Department of Veterinary Sciences and Michale E. Keeling Center for Comparative Medicine and Research,
University of Texas MD Anderson Cancer Center, Houston, TX
2Department of Immunology, University of Texas MD Anderson Cancer Center, Houston, TX
3Department of Pathology, University of Texas M.D. Anderson Cancer Center, Houston, TX
4Biostatistics and Bioinformatics Unit, University of Alabama at Birmingham, Birmingham, AL
5Department of Pathology, Huntington Memorial Hospital, Pasadena, CA
Individual classes of human endogenous retrovirus (HERV) genes
and proteins are expressed in cancer, but expression of more than
one type of HERV is rare. We report here the expression of multiple
HERV genes and proteins in ovarian cell lines and tissues. Expres-
sion of HERV-K env mRNA was greater in ovarian epithelial
tumors than in normal ovarian tissues (N 5 254). The expression of
this protein on the surface and in the cytoplasm of ovarian cancer
cells was confirmed using anti-HERV-K specific antibody by flow
cytometric analysis. The frequency of expression of HERV-K env
protein in multitissue microarrays (N 5 641) was determined by
immunohistochemistry and a significant correlation with tumor his-
totype was found. A significantly increased expression of HERV-K
was observed in tumors with low malignant potential and low grade,
relative to expression in normal ovarian tissues. The increase in
expression of HERV-K env protein took place in a stepwise fashion
in serous papillary adenocarcinoma. Interestingly, we found that
other classes of HERV env mRNAs, including ERV3 and HERV-E,
are expressed in the same ovarian cancer tissues that expressed
HERV-K. Furthermore, anti-HERV antibodies including anti-
ERV3 (30%), anti-HERV-E (40%) and anti-HERV-K (55%) were
detected in patients with ovarian cancer, but not in normal female
controls. HERV env proteins are frequently transcribed and trans-
lated in ovarian epithelial tumors, and multiple HERV families are
detectable in ovarian cancer. HERV env proteins, and especially
those expressed on the cell surface, may serve as novel tumor targets
for detection, diagnosis and immunotherapy of ovarian cancer.
' 2006 Wiley-Liss, Inc.
Key words: ovarian cancer; human endogenous retroviruses; surface
envelope proteins; anti-HERV antibodies; tumor targets
Ovarian cancer represents a great clinical challenge in gyneco-
logic oncology. The incidence of ovarian cancer is highest in the
United States and is the 5th most common malignancy among U.S.
women. There will be 22,220 estimated new cases of ovarian carci-
noma diagnosed in 2005, and 16,210 women die of the disease
annually, according to the American Cancer Society. Approxi-
mately 90% of all ovarian cancers are epithelial types. Nonepithelial
types include the sex cord-stromal tumors (6%), germ-cell tumors
(3%) and indeterminate tumors (1%).
The etiology of ovarian cancer is poorly understood. Tumor
stage at diagnosis is an important prognostic factor, and since most
ovarian tumors are not symptomatic until an advanced stage,
efforts to develop screening programs for ovarian cancer have
been undertaken. Unfortunately, the value of screening for ovarian
cancer has not been clearly established.
Human endogenous retroviruses (HERVs) and elements contain-
ing long terminal repeat (LTR)-like sequences may comprise up to
8% of the human genome.1HERVs are believed to have germline-
integrated their DNA into the human genome over 30 million years
ago. Former exogenous retroviruses may have infected the human
germ line; alternatively, infective exogenous retroviruses may have
been derived from integrated HERV sequences. HERVs contain
over 200 distinct groups and subgroups.2HERVs have been divided
into 3 broad classes3: Class 1 or g-retroviruses include HERV-E
and ERV3; class II or b-retroviruses include HERV-K and MMTV
and class III HERVs are related to spumaretroviruses. HERVs have
been grouped into classes based on sequence similarities, but the
various systems of HERV classification are somewhat confusing
and no classification scheme is universally accepted. The accumula-
tion of mutations has led to a loss of infectivity of HERVs, and in
general they are largely noninfectious retroviral remnants. However,
open reading frames (ORFs) have been observed for ERV3,4
and others10; 3 of them are functional, namely HERV-K108,6
HERV-W7and HERV-FRD.8
The ERV3 retrovirus consists of a single-copy, full-length provi-
rus that has a long ORF in the env region,11,12functional LTRs and
a nondefective env glycoprotein gene.13Eight polymorphic sites
have been found in ERV3, and 1 of them is a defective allele with a
premature stop codon.14ERV3 env transcripts are present in pla-
centa and tumor cell lines,15–18and its expression may be involved
in human vascular disease.19The gag-pol sequences of the 8.8 kb
HERV-E proviral gene, which is a human C-type retrovirus, are
?40% homologous to Moloney murine leukemia virus (MuLV)
DNA. HERV-E 4-1 mRNA expression has been observed in human
placenta,20breast carcinoma,21colon carcinoma, germ cell tumors
and prostate adenocarcinoma.22HERV-K was originally identified
by its homology to the mouse mammary tumor virus, and is tran-
scriptionally active in several human cancer tissues23–25as well as
tumor cell lines, most notably the human breast cancer cell line
T47D,26,27and breast cancer tissues, as reported by Willer et al.28
and our group.29HERV-K retrovirus-like particles have been de-
scribed that encode for viral particles in the teratocarcinoma cell
line GH,23–25and the breast cancer cell line T47D.30The K family
encodes the regulatory protein Rec (formerly designated cORF)
and is comparable to HIV-1 Rev protein.25,31–35
We previously reported that transcripts of HERV-K with coding
potential for the env region of the gene are expressed frequently in
human breast cancer,29,36and that HERV-E and ERV3 are ex-
pressed in prostate cancer.37We found that the expression of HERV
env transcripts in breast cancers was enhanced by hormone stimula-
tion, and was specifically associated with the progression of neopla-
sia.36In the current study, we evaluated HERV expression in ovar-
ian cancer cells and tissues, and report that, in contrast to most other
cancers, which express 1 or at most 2 HERVs, at least 3 HERV env
genes are expressed in ovarian cancer.
Grant sponsor: Susan G. Komen Breast Cancer Foundation; Grant
number: BCTR0402892; Grant sponsor: M.D. Anderson Cancer Center.
*Correspondence to: Departments of Veterinary Sciences and Immu-
nology, The University of Texas M.D. Anderson Cancer Center, 650 Cool
Water Drive, Bastrop, TX 78602, USA. Fax: 1512-332-5218.
E-mail: firstname.lastname@example.com
Received 24 May 2006; Accepted 18 July 2006
DOI 10.1002/ijc.22256
Int. J. Cancer: 120, 81–90 (2006)
' 2006 Wiley-Liss, Inc.
Publication of the International Union Against Cancer
Page 2
Material and methods
Cells and tissues
The human ovarian surface epithelial cancer cell lines SKOV3,
OVCA 430, OVCA 433, OVCA 420, OVCAR3, DOV 13 and
OVCA 429, and the normal human ovarian epithelial cell lines
NOE 114, NOE 116, NOE 113 and NOE 119 were gifts from Dr.
Robert C. Bast Jr., University of Texas M. D. Anderson Cancer
Center. Cells were cultured in the media recommended by this
laboratory. The immortalized normal human ovarian epithelial cell
lines T29, T72 and T80 were generated from human ovarian sur-
face epithelial cells that had been described previously.38Tissue
samples were snap-frozen and stored at 280?C until RNA isola-
tion. For in situ hybridization and immunohistochemistry, forma-
lin-fixed, paraffin-embedded tissues were used.
PCR primers
Oligonucleotide primers derived from the sequences encoding
the env surface proteins of HERV-K, ERV3 and HERV-E were
used to amplify cDNA prepared from human ovarian tissues and
cell lines as described previously.29In our study, a sense primer
(nucleotide [nt] 6674-6698; Accession number: AF07408639) spe-
cific for type 2 HERV-K env genes was also used to detect Type 2
HERV-K env mRNA transcripts, as described previously.36Previ-
ously described primer pairs were used to amplify env reading
frame transcripts that include np9.40
RT-PCR and real-time RT-PCR
RNA was prepared and total RNA was reverse-transcribed and
amplified as described previously.29The cDNA was analyzed
using primers that recognize human b-actin to confirm equivalent
loading. One-step RT-PCR was performed to quantitate the
expression of the HERV-K env gene in various ovarian specimens
as described previously.36Homo sapiens ribosomal protein S9
(GenBank accession number XM 008957.2) was used as an en-
dogenous control.41Linear extrapolation of the cycle threshold
(CT) values of HERV-K were obtained from ovarian specimens,
and then divided by the relative amounts of S9, which were quan-
titated by linear extrapolation from the CTvalues of the same
unknown samples.
Synthesis of HERV env fusion proteins and production
of anti-HERV env protein antibodies
HERV cDNAs obtained from ovarian cancer tissues were
cloned into the corresponding enzyme-digested QIA expression
vector (pQE30; Qiagen, Valencia, CA), which contains a 6-His
tag at the N-terminus. Several recombinant fusion proteins were
produced in our laboratory including HERV-K Type 1 surface env
protein, HERV-E surface env protein, HERV-K gag protein,
ERV3 env protein and HERV-K plus surface protein, which is
Type 2 env surface proteins with a 292 bp insert (also designated
HERV-K env Type 2). These fusion proteins were further charac-
terized by sequencing, followed by induction of protein expression
and purification by affinity chromatography. These purified HERV
env fusion proteins were used to immunize rabbits for production
of polyclonal, or mice for production of monoclonal, anti-HERV
env protein antibodies using standard techniques.
Flow cytometry and immunoflorescence staining
Cultured cells were incubated with anti-HERV-K antibody
(5,691; 1:200 dilution) at 4?C for 30 min, followed by anti-rabbit
IgG-FITC secondary mAb (1:1,000 dilution) at 4?C for 15 min.
For staining in permeabilized conditions, cells were treated with
0.1% Triton X-100 in PBS, then incubated with primary antibody,
followed by secondary antibody. Samples were analyzed on a BD
FACSCaliburTMsystem (BD Biosciences, San Diego, CA). For
controls, samples were incubated with anti-rabbit IgG-FITC sec-
ondary mAb. These cells were subjected to fluorescence micros-
copy to confirm the expression of HERV-K surface protein.
Immunohistochemistry of ovarian tissue slides
Paraffin-embedded ovarian tissue specimens were cut into serial
5 lm sections, melted, deparaffinized in xylene, rehydrated in
ethanol and then fixed in 4% paraformaldehyde. The slices were
incubated with anti-HERV-K env polyclonal antibody (1:200 dilu-
tion), followed by incubation with anti-rabbit IgG biotin conjugate
antibody (1:1,000 dilution) and finally with ABC (ABC kit, Vector
Laboratories, Burlingame, CA) as described by the manufacturer.
development. Slices were then counterstained with hematoxylin.
Tissue microarray slides
Multiple tissue microarray (TMA) slide TMA1, containing 72
ovarian tissues from patients with various ovarian diseases, was
obtained from US Biomax (Rockville, MD). Slide TMA2, which
contains 85 ovarian tissues that included normal, mucinous cyst,
low malignant potential (LMP) and low-grade (LG) and high-grade
(HG) carcinomas, was obtained from The University of Texas M.
D. Anderson Cancer Center Department of Pathology. TMA3 that
contains 484 cases of various ovarian cancer tissues with clinical
follow-up information was also obtained from The University of
Texas M. D. Anderson Cancer Center Department of Pathology.
Immunohistochemistry of multiple TMA slides
Immunohistochemistry was performed on multiple TMA slides
using a DAKO autostainer universal staining system. These multi-
ple TMA slides provided us with a means by which to compare
the expression of HERV-K env SU protein in multiple tissues
under identical staining conditions. The slices were incubated with
3% H2O2(5 min),horse sera (10 min) and antibodies (1:750 dilu-
tion for anti-HERV-K antibodies and 1:100 dilution for NCL-5D3
anticytokeratin antibody) (30 min). This was followed by incuba-
tion with anti-rabbit or anti-mouse IgG HRP conjugate antibody
(DAKO) (15 min), incubation with diaminobenzidine (5 min) for
color development and counterstaining with hematoxylin (5 min).
ELISA assays were used to detect anti-HERV antibodies in
human sera as described previously.42Briefly, a 96-well ELISA
plate was coated with various HERV fusion proteins (10 lg/ml,
100 ll/well) including HERV-K Type 1 surface env protein,
HERV-E surface env protein, HERV-K gag protein, ERV3 env
protein and HERV-K plus surface protein, and incubated overnight
at 4?C. After the plate was blocked, human sera (1:200 dilution)
were added and incubated overnight at 4?C. Following 6 washes,
HRP-conjugated anti-human IgG antibody (1:2,000 dilution, Sigma,
St. Louis, MO) was added and color was developed using ABTS
(Sigma) measured on a microplate reader at 405 nm. All ELISA
Expression of multiple HERV env transcripts in human
ovarian cancer cells and tissues
The expression of env transcripts of Type 1 (1,104 bp) and Type
2 (1,194 bp) HERV-K surface domains was detected in ovarian
cancer cell lines (PA1, SKOV3, OVCA 433, OVCAR3, DOV 13
and OVCA 420), but not in normal ovarian epithelial cells (NOE
113, 114, 116 and 119) or immortalized normal ovarian cell lines
(T29, T72 and T80). An example of the RT-PCR results is de-
picted in Figure 1a. The expression of multiple HERV families,
including ERV3, HERV-E and HERV-K Types 1 and 2, was de-
tected in ovarian cancer tissues at a higher frequency than in unin-
volved ovarian tissues (Fig. 1b). Expression of both Rec (437 bp;
Type 2) and np9 (256 bp; Type 1) RNA was detected in ovarian
cancer cells (DOV13 and SKOV3) and in 7 ovarian serous carci-
noma specimens (Fig. 1c). Both Rec and np9 cDNAs obtained
from DOV13 cells were sequenced and the results are shown in
Figure 1d. These results provide evidence that both types of
Page 3
HERV-K mRNA, as well as multiple HERV family mRNAs, are
transcribed in ovarian cancer cell lines and tissues.
Furthermore, 254 ovarian tissue RNAs isolated from various
ovarian specimens were quantified for the expression of HERV-K
env transcripts by real-time RT-PCR. The results of real-time RT-
PCR analyses of these samples are presented in Figure 1e. HERV-
K env expression was significantly greater in tissues from epithelial
tumors including serous, endometrioid, mucinous, clear cell, transi-
tional, squamous and mixed malignant mullerian tumor without
metastasis (p 5 0.012; n 5 121), and greater but not statistically
significant in tissues from malignant epithelial tumor with metasta-
sis (p 5 0.058; n 5 46), relative to expression in normal and benign
ovarian tissues (n 5 19), using a t-test.
Surface expression of HERV-K env protein on ovarian
cancer cells lines
Both cell surface (nonpermeabilized; No-perm) and cytoplas-
mic (permeabilized; Perm) expression of HERV-K env protein
FIGURE 1 – Expression of HERV env mRNAs in ovarian cell lines and tissues by RT-PCR. (a) Cell lines: from left to right, each set of lanes
for a given amplified gene represents the RT-PCR expression pattern using ERV3, HERV-E, HERV-K Type 1 (HERV-K(1)), HERV-K Type 2
(HERV-K(2)) and b-actin primers in OVCAR3 ovarian cancer cells (Lane 1), NOE 114 normal ovarian epithelial cells (Lane 2) and SKOV3
ovarian cancer cells (Lane 3). The final lane in each set is a no-template control (Lane 4). (b) Expression of HERV env mRNA in matched tu-
mor/normal tissues: expression of various HERV env mRNAs was evaluated in 2 cancer tissues (Lanes 1 and 3) with their matched uninvolved
normal ovarian tissues (Lanes 2 and 4) obtained from the same patients. The final lane in each set is a no-template control (Lane 5). (c) Expres-
sion of spliced HERV-K transcripts: expression of Rec or np9 spliced env HERV-K transcripts was detected in ovarian carcinoma tissues. Lanes
1–7: each lane represents an ovarian serous carcinoma specimen. The final lane is a no-template control (Lane 8). (d) Alignment of nucleotide
and amino acid sequences of Rec and np9 from DOV13 ovarian cancer cells. (e) Quantitation of HERV-K env mRNA in various ovarian tissues:
the amount of HERV-K in unknown samples was quantitated using CTvalues of HERV-K env mRNA obtained from each specimen, normalized
on the basis of the CTof Homo sapiens ribosomal protein S9. The ratio of HERV-K mRNA CTin ovarian tumor tissues to the average CTin nor-
mal ovarian control tissues was calculated. From left to right: (1) benign epithelial tumor, n 5 6; (2) epithelial tumor with low potential, n 5 5;
(3) mixed epithelial tumor, n 5 11; (4) normal ovarian controls, n 519; (5) placenta (as a control), n 5 13; (6) uninvolved ovarian tissues, n 5
12; (7) epithelial tumor with metastasis, n 5 46; (8) epithelial tumor without metastasis, n 5 121 and (9) sex cord and stromal tumor (including
germ cell tumor), n 5 21. HERV-K env expression was significantly greater (lower CT) in tissues from epithelial tumor without metastasis (p 5
0.012), and epithelial tumor with metastasis (p 5 0.058), relative to expression in normal and benign ovarian control tissues.
EXPRESSION OF HERV env GENES IN OVARIAN CANCER
Page 4
was detected in OVCAR3 and DOV13 ovarian surface epithelial
cancer cells, but not in T29 and T80 normal cells (Fig. 2a). Flow
cytometric analysis revealed greater surface expression of HERV-
K env protein on nonpermeabilized OVCAR3 (62.22%) and
DOV13 ovarian cancer cells (39.16%), than on nonpermeabilized
T29 and T80 immortalized normal ovarian cells (4–8%; Fig. 2a).
Intracellular expression of HERV-K env surface protein
in ovarian tumor epithelial cells
We next confirmed HERV-K env protein expression in ovarian
cancer tissues, and determined its association with disease stage.
Immunohistochemistry using anti-HERV-K env protein antibody
was performed on multiple tissue microarrays (TMAs). TMA1,
TMA2 and TMA3 contain 72, 85 and 484 multiple ovarian tissues,
respectively. A score of ''0'' indicates no expression, ''1'' indicates
low expression and ''2 1 3'' indicates intermediate and strong
expression of HERV-K env protein, respectively. Examples of
positively stained samples in TMA1 are shown in Figure 2b, while
HERV-K positive samples from TMA2 are shown in Figure 2c.
Expression of HERV-K env SU protein increased in a stepwise
fashion from grade I (33%) to grade II (38%) to high-grade (47%)
serous papillary adenocarcinoma (Fig. 3a) for 40 serous papillary
adenocarcinoma tissues obtained from the TMA1 microarray.
Microarray TMA2 was used for analysis of progression of ovarian
cancer (Fig. 3b). LMP serous, LG serous and LG endometrial
tumors showed higher levels of expression compared to normal
ovaries (p < 0.001). HG serous and endometrial tumors showed
great variability in protein expression with a median expression
slightly lower than normal ovaries. Furthermore, TMA3, contain-
ing 484 cases of various ovarian cancer tissues with clinical fol-
low-up information, was used to assess whether activation of
HERV-K env surface protein correlated with clinical or histologi-
cal characteristics, or with prognostic factors associated with the
patients. Tumor histotype correlated with a significant increase in
expression of HERV-K. A summary of TMA 1 and TMA3 results
is presented in Table I.
Detection of anti-HERV antibodies in sera of ovarian
cancer patients
ELISA analysis was used to detect anti-HERV antibodies in
sera obtained from ovarian cancer patients (n 5 60) and normal
female controls (n 5 50). Approximately 55% of the 60 ovarian
cancer patient samples had positive titers for antibodies against
HERV-K surface protein, 40% were positive for antibodies against
HERV-E surface protein and 30% were positive for antibodies
against ERV3 env protein. The frequency of anti-HERV antibod-
Page 5
ies from 20 sera obtained from patients with ovarian cancer and
20 normal female controls is summarized in Table II. The pres-
ence of anti-HERV env protein antibodies provides indirect evi-
dence of the presence of HERV proteins in human ovarian cancer.
In the current study, we detected the expression of both types of
HERV-K env proteins in the majority of ovarian cancer cell lines
and tissues analyzed. Sequence analysis revealed that the HERV-K
env cDNAs obtained from 2 serous adenocarcinoma ovarian cancer
specimens contained ORFs and no (Type 1; data not shown) or 1
(Type 2) stop codon. Furthermore, both HERV-K env spliced np9
and Rec mRNA were expressed in ovarian cancer tissues and the
ovarian cancer cell lines DOV13 and OVCAR 3. Rec (Type 2) pro-
tein is localized to the nucleus, and possesses functions similar to
the Rev protein of HIV.35,43Rec was reported in earlier studies to
support cell transformation and to promote tumor formation in
nude mice.34Mice that inducibly express the Rec have altered germ
cell development that may promote carcinoma in situ.44The poten-
tial impact of the unique splice variants that we have described on
ovarian tumorigenesis will require further investigation.
We also observed that other HERV classes (HERV-E and ERV3)
were simultaneously expressed in the same ovarian cancer tissues
that were positive for the expression of HERV-K. It is of interest
that multiple HERV families are transcriptionally active in ovarian
cancer, but to our knowledge not in breast and prostate cancers.
The basis of the preferential transcription of Class I (HERV-E and
ERV3) and Class II (HERV-K) env genes in ovarian cancer tissues
that we describe here is not apparent. However, the selective tran-
scription of HERV-K, HERV-E and ERV3 could be explained by
changes in tumor-specific transcription factors or tissue specific
FIGURE 2 – Expression of HERV env proteins in various ovarian cells and tissues. (a) Localization of HERV-K env protein expression in
ovarian cell lines: Surface and cytoplasmic expression of HERV-K env protein was detected in ovarian cancer cell lines OVCAR3 and DOV13,
without permeabilization (No-perm) or permeabilized with 0.1% Triton X-100 (Perm). IgG FITC antibody only was used as a negative control.
Surface expression of HERV-K env protein was detected in OVCAR3 and DOV13 cells by FACS analysis. Gray areas represent anti-IgG FITC
antibody without anti-HERV-K antibody, as negative controls. Expression of HERV-K env protein was not detected in T80 and T29 normal
ovarian epithelial cells, without permeabilization (No-perm) or permeabilized with 0.1% Triton X-100 (Perm). Surface expression of HERV-K
env protein in was detected in T80 and T29 cells by FACS analysis. (b) Expression of HERV env proteins in various ovarian tissues: scoring of
HERV-K immunostaining from TMA1 microarray: (1) normal ovarian tissues (score ''0''; 340); (2) clear cell carcinoma (score ''1''); (3) se-
rous papillary cystadenocarcinoma (score ''2'') and (4) serous papillary adenocarcinoma (score ''3''). (c) Samples exhibiting positive immuno-
staining for HERV-K from TMA2 microarray: (1) mucinous cyst; (2) mucinous LMP (low malignant potential); (3) LG (low-grade) endome-
trioid; (4) HG (high-grade) endometrioid; (5) serous LMP; (6) LG serous; (7) HG Serous and (8) clear cell carcinoma.
EXPRESSION OF HERV env GENES IN OVARIAN CANCER


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Professional Development Documents
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Should Florida's concealed-carry rules be tougher?
Published: Sunday, January 20, 2013 at 1:00 a.m.
Last Modified: Saturday, January 19, 2013 at 11:54 p.m.
Lolly Pairgin used to dust around her husband's firearms and then gingerly nudge them out of her way with a pinkie -- too deathly afraid of guns to pick up even an unloaded one.
"I'm petrified of guns," the Michigan snowbird told the staff at Bradenton's Aegis Tactical during a basic firearms course.
Four hours later, Pairgin and two of her friends were hitting eight-inch targets at 21 feet with a 9 mm Glock-19.
Now trained, they can apply for a concealedcarry license in Florida.
Aegis Tactical's owner and lead trainer, Joe Krawtschenko understands the seriousness and vicarious liability of firearms training. He teaches his students not only how to shoot, but also when state law says deadly force can be used.
Pairgin and her friends chose to learn how to use their weapons from someone who gave them authoritative training, but that level of instruction is far from standard in Florida, where more than a million residents have concealed-weapon permits costing $112 apiece.
Some trainers use toy guns or allow students to pull the trigger on a BB-gun, a power drill or a Windex bottle. A few will sell a certificate of competency to a student with no training, experts say. Krawtschenko has had students in his firearms training classes who have never touched a gun even though they've had a concealed-carry license for years, obtained from a less scrupulous instructor.
At a time when gun ownership has been driven to the forefront of American consciousness by the school shooting in Connecticut and by the Obama administration's recent proposals for reducing gun violence, state law is vague on the specifics of training required for a concealed-carry permit. It says only that students must "demonstrate competence" with a gun, leaving much to be interpreted by the thousands of instructors operating independently in the state; their exact number is not known.
Krawtschenko and other instructors are pushing for strengthened requirements for obtaining this type of permit, such as adding a qualification course and lesson plans. They join a long list of proponents who have pushed for such changes over the years.
"I'm signing off on their license," Krawtschenko said. "If, God forbid, they use it, an attorney will be knocking on my door. I can't do what these other instructors are doing."
But opponents, including the National Rifle Association, say these instructors have a vested interest in more expansive regulations.
They "have a financial interest in imposing additional restrictions on Second Amendment rights," said Marion Hammer, the NRA's Florida chief and the first female president of the national organization.
Representatives from Florida Carry.org, a statewide gun rights group, say any additional regulation of training requirements for concealed-carry permits is a meander down a "slippery slope."
Managers at the Florida Division of Licensing, the state agency charged with issuing concealed- carry licenses, acknowledge that they have no authority to regulate how someone is trained, much less police the trainers themselves. The extent of their power is to deny the license application.
Ken Wilkinson, the division's assistant director, said when his staff learns of a trainer using questionable practices, they stop issuing licenses to the students. "We don't hear that many horror stories, but that doesn't mean it doesn't happen," he said.
Wilkinson declined to comment on the merits of elevated training standards.
"My job is not to have an opinion on the matter," he said. "My job is to run the division, and follow the directives of the director and commissioner."
But Wilkinson pointed to the concealed-carry permit requirements in South Carolina -- state-certified instructors, a mandatory eight-hour class with lesson plans, and insistence that applicants demonstrate their abilities at a gun range -- as a program that is working well.
Some states have more rigorous standards than South Carolina, while only a handful have more relaxed requirements than Florida.
Covering the basics
Krawtschenko is a former deputy sheriff from New Jersey who worked as a civilian contractor in Afghanistan, There, he taught firearms and tactics to U.S and foreign military. When he started Aegis about a year ago, he sought out other trainers with similar experience.
Instructor Chris Ganley fought overseas as an infantryman, and served as a firearms instructor for the Marine Corps. Kevin Murphy retired after 27 years in law enforcement and taught criminal justice to high school students.
The instructors know most entry-level students are not seeking commando-style expertise.
When Pairgin and friends Carmen Duquette and Carmen Myers took their basic firearms course, Murphy and Ganley kept things light, informative and fun. There were no war stories and minimal technical jargon.
Duquette, a snowbird from Canada living in Tennessee, wants to carry a pistol when she hikes in the Smoky Mountains near her home. To do that, she needs a carry permit issued by the state; her training and concealed-carry license here in Florida will allow her to meet those requirements.
"I would feel a lot more secure with a gun," she said.
Myers, like Pairgin, never handled a weapon before the course and was similarly afraid of her husband's guns. "I had this nightmare -- someone breaks in and I didn't know what to do with the guns," she said.
"You'll be nervous, but you'll overcome that if you want to survive," Murphy explained.
Using a lesson plan developed in-house, the instructors patiently covered the rules for safe gun handling and basic fundamentals of marksmanship.
Murphy even explained the intricacies of shooting with bifocals.
He took the students through several scenarios -- the kind of shoot/don't shoot training given to most police recruits. All three women said they would likely shoot if they saw a middle-aged man jump out of his car, run toward a young girl and drag her kicking and screaming back into his vehicle.
But this wasn't a two-dimensional question. "What if it's his daughter and she had just run away from home?" Murphy asked.
The three novice shooters could have chosen an instructor who lectured for 15 minutes and watched them fire a BB into a bucket of sand.
Krawtschenko knows he is losing business to those kinds of trainers, who churn out students faster and cheaper.
He has written to the governor's office and the licensing division seeking licensing reform -- efforts that have produced little results. "There's got to be mandatory qualifications for obtaining a concealed carry license," Krawtschenko said.
Over the years, other instructors have lobbied for similar change. In a 2006 letter sent to the governor's office and the licensing division, retired Col. James Otto, Sr., a firearms instructor, outlined his concerns about the three-hour licensing classes taught at gun shows. Otto noted that his training classes take three to seven days.
"As an NRA certified firearms instructor and range safety officer, I can no longer trust those carrying permits until I inquire as to their instruction," Otto wrote.
The licensing division told him it had no regulatory authority over instructors, and was "not empowered by law to prescribe or enforce standards of course or content."
War stories
Stories of poor training are easy to come by.
In a 2009 complaint letter to the licensing division, Fort Myers attorney J. Patrick Buckley III warned of a gun shop class where, after only 90 minutes of training, students either fire a blank at a Dumpster behind the business or "in the store's restroom, at a toilet."
Charlotte County Sheriff's Lt. Brian Harrison knows of one gun shop that offers classes where students fire a couple of rounds into a clearing barrel behind the shop.
His agency has offered residents a comprehensive firearms course for years.
A SWAT team commander and certified firearms instructor, Harrison said he would "100 percent support" strengthening mandatory training requirements statewide.
The sheriff's office course is eight hours long, costs only $25 and fills up quickly.
"I'd like to offer the course for free, as a public service," Harrison said. "Some gun stores charge $100 and knock it out in under an hour."
But he NRA and other opponents of any changes to the rules say such revisions, however well- intentioned, can do more harm than good.
Before 1987 and the passage of the statewide law governing concealed-carry, each county was allowed to issue its own license.
"We had 67 counties doing it 67 different ways," said Hammer, executive director of the Unified Sportsmen of Florida, the state's legislative NRA affiliate.
Some counties refused to issue any licenses, Hammer noted. Monroe County had a nonrefundable $2,200 application fee. Dade and Broward charged $500 and required a certificate from a psychiatrist attesting that the applicant was "mentally suited to carry firearms," she said.
"The county got to pick the psychiatrist, and you had to pay the bill," she said.
Hammer, who also served as NRA president from 1995 to 1998, crisscrossed the state, drumming up support to change the law. She does not want anyone tinkering with the current statute over an issue that she said "crops up regularly."
"Instructors tend to think they know what's best. That's not always true," she said. "This legislation was passed in 1987. In all those years, there's never been a single recorded case of an accident or incident with a concealed weapons license holder that resulted from a lack of training."
The NRA is aware some of its Florida instructors were taking shortcuts, and has taken steps to address that, she said, but there is little recourse for students other than a civil suit against their trainer.
Hammer said some instructors have lobbied for legislation that "embodies their specific course."
"The would like the Legislature to do their marketing for them," she said. "They can't demonstrate we need to change the criteria."
Some states require much more training before issuing a concealed carry permit, while others require no training at all, said Sean Caranna, founder and executive director of Florida Carry Inc., a 9,000-member nonprofit formed to contest "ill-conceived gun control laws."
"Florida struck a good balance," said Caranna, who said he is worried an "arbitrary" training requirement could erode Second Amendment protections.
"What's the limit?" he said. "There are people in Tallahassee who will say if you haven't taken an 18-week infantry course, you're not qualified to carry a weapon."
"It's a slippery slope," he said. "It would be like saying that before you can write a letter to the editor, you need to take a journalism course."
This story appeared in print on page A1
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The form of the narrative poem is probably the earliest written record of the English language as it emerged during the middle ages. The poets were intelligent and inventive and the scraps and... More > fragments that remain for study continue to fascinate and amuse readers and listeners today. Simon Kellow-Bingham has employed a similar method as these ancient rhymers to bring an old story to a new audience. The Stonecutter's Tale takes two stories, that of Saint Erkenwald, an anonymously penned 14th Century alliterative poem and the timeless folk story, The Peddler Of Swaffham, and brings them together into twenty-five verses of rhyming couplets. First performed in 2001 The Stonecutter's Tale has been refined over ten years to become the poem within these pages. This edition includes an updated introduction and note on the text with a select bibliography for further reading.< Less
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OregonLive: OSU Football News
- December 31, 2014
( 43)
OregonLive: Oregon State Beavers Football News app gives OSU fans exclusive access to the latest news, videos and photo slideshows from OregonLive. Read exclusive articles about the Beavers and other rival teams, catch live Twitter updates from fans and experts, view and submit photos from the game.
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Download the OregonLive.com: Oregon State Beavers Football News app today!
PLEASE NOTE: This App is developed and published by Advance Digital Inc. and OregonLive.com. The App is not endorsed, licensed or sponsored by Oregon State University or National Collegiate Athletic Association.
Worst running app ever Slow and glitchy. The O needs to bring in some college interns to fix their embarrassingly poorly engineered apps.
Doesn't work All it displays is a grey box. App is unresponsive. It was fine before last update. Using moto x.
Beavers app is great! Get my Beavis fix all year long from this and hoops app. GO BEAVS!
Works great for me! Works great on my Samsung Galaxy S4. The only reason I wouldn't give this app five stars is because there are too many OSU basketball stories. It's supposed to be FOOTBALL NEWS, people!
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CHIL has unveiled the US$39.99 Notchbook for iPad Air. Its patent-pending "Notch" design is built to open up the case to allow room for the user's hand to slide into the case and nestle around the tablet, providing a secure grip.
The Notchbook also features a platform that rotates 360° and doubles as a folio case and iPad stand. Available in five colors — black, English tan, teal, violet and merlot — the Notchbook is constructed with padded leather with a microsuede interior delivers a smooth finish that keeps your iPad scratch-free. It's available for purchase at Amazon.com, InMotion Stores, CHIL.com, and other retailers.


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A letter writer to a Minnesota newspaper, contending there's an epidemic of big-rig drivers' speeding on the nation's highways, is suffering from tunnel vision when he mocks drivers' "professional" badge of honor.Read More
Politicians making ridiculous comparisons notwithstanding, in the late call for regulation of GPS units, many readers noted there could be no technological excuse for a lack of driver common sense.Read More
What is the best way to use a stake pocket as a chain anchor point? Reader J.D. Bronson, among others, offers his preferred method.Read More
Owner-operator Mike Crawford is approaching 3 million safe miles all logged in the same 1994 Freightliner -- wish him luck on the final 37,000...Read More
The Federal Motor Carrier Safety Administration's new Facebook page could be a great venue for drivers to get their viewpoints heard by the general public. Also: detail on an upcoming public meeting of the Motor Carrier Safety Advisory Committee.Read More
This comes by way of the Facebook page for the Big Road app (shared from that of CDLLife.com), which I wrote about yesterday. Anti-texting billboards are probably the wrong place for invitations to text for more info... Happy Sunday, folks.Read More
Owner-operators' No. 2 named challenge, the unsettled nature of the hours of service regulations and a potential electronic logs mandate, has moved some to embrace change, others to fight for fairness.Read More
At U.S. House hearing on CSA, concerns about driver pay were aired with contentions that better nondriving-time compensation was much needed.Read More
NASTC president David Owen answered the question in the association's new entrants seminar July 27, 2012.Read More
A suit against the FMCSA's CSA program brought by the ASECTT group alleged unfairness to small carriers within FMCSA's compliance and enforcement regime.Read More


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- Aug 20:
- Marshall rift with Broncos getting bigger
- Krieger: Broncos' new slogan: We know drama
- Dove Valley Days: Two rookie DBs on the rise
- Smith goes from tense to traded
- Despite his cast, safety Dawkins still making plays
- Aug 12:
- Dove Valley Days: Smith bolstering Broncos' secondary
- Torain done with the Broncos after another knee injury
Ryan Harris didn't miss an offensive snap last year, let alone a game.
The Broncos' right tackle might miss the preseason opener Friday at San Francisco after injuring his right shoulder Monday during the team's practice at Invesco Field at Mile High.
The team won't know the full extent of Harris' shoulder injury until today, although initially it didn't appear serious enough to put his regular-season playing streaks in jeopardy. Harris may even try to get in a practice before the team leaves for San Francisco.
A third-round pick from Notre Dame in 2007, Harris overcame back surgery during his rookie training camp to play in 11 games. Switched from left to right tackle last season to make room for Ryan Clady, Harris started all 16 games in 2008 and lined up for all 1,019 snaps.
When Harris left the practice field Monday, he was replaced at right tackle first by Brandon Gorin and then Tyler Polumbus.
The Broncos signed Polumbus last year from the University of Colorado as an undrafted free agent. He has taken most of his reps as a second-team left tackle behind Clady.
Gorin missed all of last season with a shoulder injury, but played for McDaniels in New England from 2002-06. Gorin started 23 games during that span, including the Patriots' Super Bowl XXXIX defeat of Philadelphia after the 2004 season.
"I'm familiar with the system, so that helps me with the learning curve," Gorin said. "But football is football no matter where you go. As an offensive lineman, you either block the guy in front of you or you don't.
Since ending his short contract holdout last week, rookie outside linebacker Robert Ayers has received his share of loud instruction.
There was a day last week when McDaniels got in Ayers' face. And during the team practice Monday at Invesco Field, linebackers coach Don Martindale was heard yelling at the prized new defensive player.
"They're not just yelling because I'm a rookie. They're yelling because I need to improve," Ayers said.
Footnotes. The Denver Post's NFL reporters post analysis, notes and minutiae on this blog devoted to the Denver Broncos.
Blog: All Things Broncos
The Denver Post's NFL reporters post analysis, notes and minutiae on this blog devoted to the Denver Broncos.
Running back LaMont Jordan did not practice Monday because of an unspecified injury. . . . The Broncos weren't hurting for birthday wishes Monday. Nick Greisen (30), Matt Prater (25) and Ryan Torain (23) turned a year older. Cornerback Andre Goodman turns 31 today. . . . Rockies radio announcer Jack Corrigan stopped by practice Monday to catch up with McDaniels. In a previous life, Corrigan was an assistant football coach for St. Ignatius High School in Cleveland and went up against Josh and Ben McDaniels, both of whom played quarterback for Canton McKinley High School.
Mike Klis and Lindsay H. Jones, The Denver Post


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Success in your calculus course starts here! James Stewart's CALCULUS texts are world-wide best-sellers for a reason: they are clear, accurate, and filled with relevant, real-world examples. With CALCULUS: EARLY TRANCENDENTALS, Sixth Edition, Stewart conveys not only the utility of calculus to help you develop technical competence, but also gives you an appreciation for the intrinsic beauty of the subject. His patient examples and built-in learning aids will help you build your mathematical confidence and achieve your goals in the course!
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The late James Stewart received his M.S. from Stanford University and his Ph.D. from the University of Toronto. He did research at the University of London and was influenced by the famous mathematician George Polya at Stanford University. Stewart was most recently Professor of Mathematics at McMaster University, and his research field was harmonic analysis. Stewart was the author of a best-selling calculus textbook series published by Cengage Learning, including CALCULUS, CALCULUS: EARLY TRANSCENDENTALS, and CALCULUS: CONCEPTS AND CONTEXTS, as well as a series of precalculus texts.
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Book Description Brooks Cole, 2007. Book Condition: New. Brand New, Unread Copy in Perfect Condition. A+ Customer Service! Summary: 1. FUNCTIONS AND MODELS. Four Ways to Represent a Function. Mathematical Models: A Catalog of Essential Functions. New Functions from Old Functions. Graphing Calculators and Computers. Review. Principles of Problem Solving. 2. LIMITS. The Tangent and Velocity Problems. The Limit of a Function. Calculating Limits Using the Limit Laws. The Precise Definition of a Limit. Continuity. Review. Problems Plus. 3. DERIVATIVES. Derivatives and Rates of Change. Writing Project: Early Methods for Finding Tangents. The Derivative as a Function. Differentiation Formulas. Applied Project: Building a Better Roller Coaster. Derivatives of Trigonometric Functions. The Chain Rule. Applied Project: Where Should a Pilot Start Descent? Implicit Differentiation. Rates of Change in the Natural and Social Sciences. Related Rates. Linear Approximations and Differentials. Laboratory Project: Taylor Polynomials. Review. Problems Plus. 4. APPLICATIONS OF DIFFERENTIATION. Maximum and Minimum Values. Applied Project: The Calculus of Rainbows. The Mean Value Theorem. How Derivatives Affect the Shape of a Graph. Limits at Infinity; Horizontal Asymptotes. Summary of Curve Sketching. Graphing with Calculus and Calculators. Optimization Problems. Applied Project: The Shape of a Can. Newton's Method. Antiderivatives. Review. Problems Plus. 5. INTEGRALS. Areas and Distances. The Definite Integral. Discovery Project: Area Functions. The Fundamental Theorem of Calculus. Indefinite Integrals and the Net Change Theorem. Writing Project: Newton, Leibniz, and the Invention of Calculus. The Substitution Rule. Review. Problems Plus. 6. APPLICATIONS OF INTEGRATION. Areas between Curves. Volume. Volumes by Cylindrical Shells. Work. Average Value of a Function. Review. Problems Plus. Appendixes. A: Intervals, Inequalities, and Absolute Values. B: Coordinate Geometry and Lines. C: Graphs of Second-Degree Equations. D: Trigonometry. E: Sigma Notation. F: Proofs of Theorems. G. Complex Numbers. H: Answers to Odd-Numbered Exercises. Bookseller Inventory # ABE_book_new_0495384178
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Book Description Brooks Cole, 2007. Hardcover. Book Condition: New. 6. Bookseller Inventory # DADAX0495384178
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Book Description Brooks Cole. Hardcover. Book Condition: New. 0495384178 AtAGlance Books -- Orders ship next business day, with tracking numbers, from our warehouse in upstate NY. This book is in brand new condition. Bookseller Inventory # 9780495384175N
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Book Description Brooks Cole, 2007. Hardcover. Book Condition: New. Bookseller Inventory # P110495384178
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Reviewed By Tom P (SEAFORD, NY) Reviewed for a 2009 Ford F-150 —8/24/2014 11:57 AM
Quick and easy to install.
Reviewed By Travis R (Alderson, WV) Reviewed for a 2009 Ford F-150 —4/20/2011 2:52 PM
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It gives great protection to the box on the truck. This week we had a record setting snow storm and I didn't have to shovel out my truck. The cover is very easy to clean off. We haven't driven the truck very far since getting the cover, but I am sure that we will get better gas mileage. It looks great on the truck.