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|Reviews for Silence
| Rori Potter chapter 5 . 12/30/2010
That was amazing. Update soon.
| GundamPilot03 chapter 5 . 12/30/2010
Aww! That was so cute. I loved it. I can't wait for the next chapter.
| Rori Potter chapter 4 . 12/29/2010
That was amazing. Update soon.
| GundamPilot03 chapter 4 . 12/29/2010
Awesome chapter. I can't wait for the next one. They are so cute in this.
| Tillififf chapter 4 . 12/29/2010
he's getting closer~ 3 hurray! I'm hopping around here, hoping he'll catch his slytherin!
| sonata hirano chapter 4 . 12/29/2010
awwwwwwwwwwwwww he couldn't fall asleep without holding Harry sooo CUTE!
| allyct160 chapter 4 . 12/29/2010
can't wait for more and looking like a great story!
| GundamPilot03 chapter 3 . 12/27/2010
I loved this chapter. It was awesome. I can't wait for the next one. Are we ever going to find out exactly what the Death Eaters did to Harry or how he saved Snape? I hope so. I'm curious.
| allyct160 chapter 3 . 12/27/2010
so far so good and he'll soon have his voice back too!
| Rori Potter chapter 3 . 12/27/2010
That was amazing. Update soon.
| Tillififf chapter 3 . 12/27/2010
Snarry ftw and thus the slytherin potions master fell for his gryffindor co-worker. I really like the bits when they step it up, shame he had to work _ great chapter! :D
| tatertot72015 chapter 3 . 12/27/2010
That was soo totally good please keep going, I can't wait to read more.
| Tillififf chapter 2 . 12/27/2010
Oooh I like this story I think it's a fun idea that Harry's lost his voice and Snape tries to get it back. Yay, can't wait for next chapter
| Yamahasi chapter 2 . 12/23/2010
Wonderful story! Very instresting plot! But I do kind of find it ironic that Severus asked Harry to shut up and suddenly, BAM! Not able to speak. Love that though, makes it all the more mysterious, a real mystery. Anyway can you pretty please write the next chapter soon? I'm dying of curiousity to see what happens next. Thanks!
| lady.edgecombe chapter 2 . 12/21/2010
Very interesting. Don't often find new concepts. Can't wait to see what happens next.

subset: commoncrawl


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Player power rankings: Brodeur rockets
Good week? Brodeur became the all-time minutes leader and shot up the rankings.
Originally Published: November 30, 2009By Tom Awad | Puck Prospectus
The Puck Prospectus metric GVT (goals versus threshold) allows us to compare players at every position to determine who the true leaders are. As December kicks off, the GVT rankings were shaken up by two NHL superstars who boosted themselves into the top 10. Martin Brodeur jumped 13 spots to No. 2 and Sidney Crosby jumped 12 spots to No. 6.
To see which players lead the league on the ice, you must be an ESPN Insider.
Become an insider and get access to all our articles and tools.View Insider Benefits »
Magazine subscribers: activate your Insider account.
- PP Weekly: The East at the quarter pole
- PP Weekly: The West at the quarter pole
- PP Weekly: Avs are smart spenders
- PP Weekly: Absence of Ovie, stars won't hurt
- MacInnis teaches Johnson art of shot-pass
- NHL's sensational sophs lend advice to rooks
- Explaining Mag Power Ranking categories
- The Mag: NHL Power Rankings


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Les Moonves, Dick Wolf and Ron Howard Among TV 'Hall of Fame' Inductees
The Academy of Television Arts & Sciences will also honor CBS' Bob Schieffer and sportscaster Al Michaels at the ceremony on March 11.
Les Moonves, Dick Wolf and Ron Howard are among the honorees to be inducted into The Academy of Television Arts & Sciences' Hall of Fame this year.
CBS CEO Moonves, Law & Order mastermind Wolf and multi-hyphenate Howard will be joined in receiving the honor along with CBS broadcaster Bob Schieffer, sportscaster Al Michaels, and, posthumously, Philo T. Farnsworth, the once-overlooked inventor who created all-electronic television transmission.
The 22nd annual gala ceremony will take place March 11 at the Beverly Hilton. For the first time, proceeds from the event will benefit the Television Academy Foundation's Archive of American Television, an ever-evolving collection of video interviews with the medium's pioneers and legends.
"Each of this year's Hall of Fame inductees is incredibly deserving of this honor and is truly a legend of our industry," said Academy chairman and CEO Bruce Rosenblum in a statement Wednesday. "This will be a spectacular evening, rich with stories and reminiscing."
Boldfaced names on the Hall of Fame selection committee include Warner Bros. Television president Peter Roth, NBCU Cable Entertainment leader Bonnie Hammer and CBS Entertainment president Nina Tassler.
Never miss a story. Stay up-to-date with THR's Daily Headlines.
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The list below identifies those cases in which the Court has granted the leave application or has ordered a mini-oral argument on the application ("MOAA") but which have not yet been argued at a session of the Court. Once scheduled for argument, the cases will be removed from this page and included on the Schedule of Oral Arguments page. Attorneys whose cases appear on this page should immediately notify the Clerk's Office in writing of vacation plans or other commitments that might conflict with the Court's argument dates. Although providing a timely notice of conflict is no guarantee that the case will not be scheduled for argument, every effort will be made to accommodate the attorney's vacation or commitment.
The "LG" or "MOAA" in the left-hand column denotes whether the case is on leave granted or to be argued on the application, respectively. The leave granted or MOAA orders can be accessed by clicking on the "LG" or "MOAA" designation for a case. The right-hand column contains links to those pleadings filed to-date (attachments to the pleadings are not included). The pleadings in support of or opposition to a leave application are included for cases in which the briefs are yet to be filed. As the briefs are filed, they will be added to the right-hand column and the links to the application materials will be removed. For MOAAs, the leave application materials will remain linked even when supplemental pleadings are filed per the direction of the Court.
Parties and amici curiae are required to file their briefs within the deadlines specified in the Michigan Court Rules. See MCR 7.306(D)(1) and MCR 7.309(B)(1)(a) & (2)(a). Motions to extend time should be filed in advance of the filing deadlines and will be granted only upon a showing of good cause. MCR 7.316(B).
The list can be sorted in ascending or descending order by order date and docket number. Click on the arrow to the right of the order date and docket number headings to select the desired sort order. NOTE: Only the first 30 cases are listed on this page. If there are more than 30 cases awaiting oral argument, click on the right-facing arrow at the bottom of the screen to open the next page of cases.
People of MI v Paul Seewald
COA maj opn
COA dissent
AT brf
AE brf
AC brf - Pros Atty Assn of MI
COA opn
People of MI v Sean Harris, William Little, Nevin Hughes
COA opn
COA concur-dissent
149872-3 AT brf
150042 AT brf
149872-3,150042 AE brf
AC brf - POLC
AC brf - POAM
Wyandotte Electric Supply v Electrical Technology Systems
COA opn
AC brf - Assoc Gen Contractors of MI
People of MI v Dennis Tomasik
COA maj opn
COA concur
AC brf - PAAM
People of MI v Charles Almando-Maurice Dunbar
COA concur
Appl supp
AC brf - PAAM
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Alan Jesperson v Auto Club Ins Assn
AE brf
Glenn S Morris v Morris Schnoor & Gremel, Inc
COA opn
Ronnisch Construction Group v Lofts on the Nine, LLC
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Jeffrey Cullum v Frederick Lopatin, DO
AT supp brf
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Roberto Landin v Healthsource Saginaw, Inc
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AC brf - MHHA
People of MI v Brandon Michael Hall
People of MI v Kameron Leo Kilgo
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David Abbo v Wireless Toyz Franchise, LLC
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Dustin Rock v Dr K Thomas Crocker
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People of MI v John Oliver Wooten
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People of MI v Robin Scott Duenaz
In re Appl of Consumers Energy for Reconciliation of 2009 Costs
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People of MI v Stephanie White
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People of MI v Yumar Antonio Burks
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Helen Yono v Dep't of Transportation
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Earl Allard, Jr v Christin Allard
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Innovation Ventures, LLC v Liquid Manufacturing, LLC
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People of MI v Michael Andrew Radandt
Lindita Pirgu, Conservator & Guardian of Estate of Feridon Pirgu v United Services Automobile Ass'n
COA concur-dissent
Kimberly Corl, PR of the Estate of Bradley Corl v Huron & Eastern Railway
Craig Hecht v National Heritage Academies, Inc
COA dissent
Lale Roberts v Kathryn Salmi, LPC


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Are walkers allowed on the Boston Marathon course?
Only officially registered participants are allowed on the course. The course and finish area facilities close six hours after the last runner crosses the starting line. The Boston Marathon does not have a race walk division.
How do I enter the Boston Marathon through the Charity Program?
To apply to run for a BAA official charity, please contact your charity of interest directly. More information regarding the B.A.A charity program can be found by clicking the button below:
Each charity coordinator's contact information is linked to their charity's icon, and you can contact them for application instructions. These charities are currently accepting applications, and we suggest that you apply at your earliest convenience if you are interested in running for one.
Will I get disqualified if I start in the wrong wave of the Boston Marathon?
You may move from your assigned Wave to a later Wave. For example, if you are assigned to Wave 1, you may move back to Wave 2, Wave 3, or Wave 4 in any corral EXCEPT the first corral in either Wave. However, you may NOT move to an earlier Wave. For example, you may not move from Wave 4 to Wave 3, from Wave 3 to Wave 2, or from Wave 2 to Wave 1. Violators are subject to penalties and/or disqualification.
Are B.A.A. 5K awards presented according to official ("gun") time or net time?
All prize money winners will be scored by gun time. All age group division awards will be awarded by net time.
Are bikes/strollers/skates/baby joggers/dogs allowed on the course of the B.A.A. 5K?
No. For safety reasons, strollers, in-line skates, skateboards, and vehicles of any kind, as well as animals, are not permitted.
Will the Boston Marathon be broadcast on television and radio?
Yes, the race will be broadcast live on television nationally on Universal Sports Network and locally on WBZ-TV. The Boston Marathon will also be streamed live online at http://watchlive.baa.org/.
All of the above is subject to change.
How do I get party tickets to the pre-race or post-race parties?
A complimentary ticket is included with your bib number packet. A limited number of additional tickets for both the pre-race dinner and post-race party may be purchased at the "Party Tickets" Booth when you pick up your number. Tickets will NOT be available for sale on site at either event.
What do I need with me when registering for the Boston Marathon?
To register for the Boston Marathon, you will need to provide the following information on the registration form:
What is the best way to make Boston Marathon hotel reservations and how do I know where the hotels are in relation to the finish area?
1966 - Bobbi Gibb
Although not an official entrant, Roberta "Bobbi" Gibb became the first woman to run the Boston Marathon. Joining the starting field shortly after the gun had been fired, Gibb finished the race in 3:21:40 to place 126th overall. Gibb again claimed the "unofficial" title in 1967 and 1968.


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Radisson Blu Royal Garden Hotel, Trondheim
No prices found through our partners. Please contact the business directly or check some of our recommended alternatives.
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- Continental Breakfast
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TravelPod Member ReviewsRadisson Blu Royal Garden Hotel, Trondheim
Nice modern hotel right along the canal in city center Trondheim. Close to the rail station.
This review is the subjective opinion of a TravelPod member and not of TravelPod.com.
TripAdvisor Reviews Radisson Blu Royal Garden Hotel, Trondheim
Travel Blogs from Trondheim
Off the Boat
We disembarked today in Trondheim at 8:30 this morning and quickly discovered that just about everything closes on Sunday in Trondheim. We did find a few restaurants open, but their local micro-brewery and what appeared to be a promising antique store were both closed.
So we had a nice quiet and sunny day to relax, wind down and get ready to board the train tomorrow for Oslo.
Trondheim, the original capital
... hours there. There was also an indoor museum. After walking back into town, we hiked up another steep hill to the Kristiansten fortress. It was built in the C17 as a way to protect the city from attack (there have been plenty of these). In WW2 it was taken over by the Germans as a place to perform executions. Many of the Norwegian Resistance were shot there. A long day all in all. Off to Oslo ...
A monastery and a WWII gun
... smorgasbord, but there wasn't anything there that you could recognise as Norwegian.
Went to use the 'loo with a view'. It is great sitting on the loo and watching the world glide by. It also makes it better than using the toilet in the cabin.
It is now 10pm and broad daylight outside – or pretty light anyway and it is time to shower and go to bed. Whoo hoo the shower was ...
From the Medieval to Modern Marvels
... settlements and fishing villages. At 4-15 we were called onto the deck to watch our approach to Kristiansund. This was spectacular as we turned from the main fjord into the long harbour, under a towering bridge and seemed to sail right into the heart of the town. The cliffs overlooking the sound were dotted with wooden houses and there were plenty of small ships moored in the marina.
Here we disembarked for our second excursion of the day. ...
Kystekspressen to Trondheim then train to Sweden
... promising. Nothing on the other side; nothing to the left, so we went right through the mud and the road works.... Right decision - came across a bridge over the railway lines to the railway station! Eventually found the lockers (the girl on information was new - she got to know us by the end of the day!); deposited our bags in said lockers; found out we couldn't reserve a seat on the train to Ostersund; got a map - then over the bridge crossing the Nid ...


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Navy Lawyer Expands Horizons at Sea Practicing Operational Law
Story Number: NNS140207-09Release Date: 2/7/2014 10:55:00 AM
By By Lt. Theresa Donnelly
USS BOXER, At Sea (NNS) -- From leaving the turmoil of a war-torn nation following the break-up of the Soviet Union, to now teaching thousands of Navy and Marine Corps operators in the Boxer Amphibious Ready Group (BOXARG) the international law of armed conflict, Lt. Givi Tibaneli, a Navy Judge Advocate, is on an eight-month deployment practicing a unique and specialized form of his profession.
Emigrating at the age of 10 from the country of Georgia, Tibaneli says his experiences witnessing lawlessness and corruption propelled him to direct his studies to law and policy as well as contribute to his new country.
"I've always held the rule of law in high regard and how those laws were implemented throughout the world," said Tibaneli. "Serving my country was a way for me to give back to the United States for the opportunity that this country provided my family," he said.
Giving back to others has always been a part of Tibaneli's life. While attending University of California, Hastings College of Law, Tibaneli volunteered with the "Hastings to Haiti Partnership," an organization that was devoted to advancing the rule of law and human rights in Haiti by supporting the country's legal education and engaging in human rights advocacy. He helped the group through fundraising for annual working trips to the country, hosting clinics and performing legal research.
"The whole idea there was that if you were to empower and plant a root in the legal education sector, to these students who would one day be the future judges and prosecutors in that country, they could influence change by affecting the way laws are implemented and enforced," said Tibaneli.
After graduating from law school, Tibaneli chose to join the Navy because he wanted to serve and practice operational law. Operational law encompasses multiple legal areas such as international treaties, the law of the sea, rules of engagement, environmental law, as well as administrative law and ethics. It also involves cooperation and coordination with host nation governments and U.S. embassies in order to successfully implement and enforce liberty policies.
Operational legal counsel is crucial in the Navy, which deals with complex missions and politically sensitive topics such as disputed territories, strait transits and multiple Status of Forces (SOFA) agreements with host nations.
Following his studies in law school, Tibaneli served for approximately a year and a half as trial counsel (prosecutor) at Navy Base Ventura County. While stationed there, he honed his skills practicing law in the courtroom, but his ultimate goal stayed intact.
Recognizing his interests and potential, Tibaneli was nominated by his commanding officer at the Navy Region Legal Service Office (RLSO) to leave his first tour early and report as the Staff Judge Advocate (SJA) for Commander, Amphibious Squadron (CPR1). CPR-1 is composed of three amphibious assault ships that carry Marines and aircraft in order to perform missions across the full spectrum of military operations.
"My experiences here have been very positive. I've enjoyed it both professionally and personally. I particularly enjoy operational law because it's something that is unique and can't be practiced outside the Navy," said Tibaneli.
Shortly after the Boxer left for deployment, Tibaneli took on the challenge of qualifying as the Officer of the Deck (OOD) in an effort to better understand the circumstances naval officers face while operating a warship. He has already completed initial watchstander qualifications and time permitting, hopes to become fully qualified by the end of deployment.
Obtaining his OOD qualification means that Tibaneli will have a first-hand perspective of the legal challenges ship handlers face while operating at sea.
"I noticed when I was giving briefs to watchstanders about Rules of Engagement and various scenarios that people listen more carefully when you have your own experiences to draw upon, which makes you a better lawyer. Studying for OOD makes me a better legal advisor and naval officer," he said.
Tibaneli hopes that his approach to his trade will encourage more operators to proactively seek legal counsel and understand how his services can be used as a valuable resource for the staff.
"Providing good legal advice offers the commander a much more comprehensive analysis of the problem prior to making a decision. It's a misperception when people think that you can only go to legal when the situation has gone bad," he said.
For more news from USS Boxer (LHD 4), visit www.navy.mil/local/lhd4/.
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Home » Local News » USA Today: Traverse City State Theatre a 'magical place' to see a movie; What's your favorite?
Posted on 13 July 2012. Tags: 'magical, City, favorite, movie, place', state, Theatre, today, Traverse, What's
The historic State Theatre in Traverse City is one of the nation's "10 greatest places to see a movie in splendor," according to USA Today Travel.
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|Trad, Aid, 1 pitch, 120', Grade II
|YDS: 5.10+ French: 6b+ Ewbanks: 21 UIAA: VII+ ZA: 20 British: E3 5b A1 [details]
|Jason Keith and Hollis McCord
|spring, summer, fall
|Page Views:
|Submitted By:
|Sam Lightner, Jr. on Jun 1, 2008
|Comments on Hollis Route
|Add Comment
|Show which comments —
By rob pizem
Apr 20, 2010
I freed the Hollis route this spring. It was cold, sandy, crumbly and fun.
Gear is from fingers to a number 5.
Out the cave roof start one or two finger pieces to a .5 and a .75 or two into tight hands and then hands to the wide stuff.
Great way to ascend the wall to see the potholes on top!
1 70 meter was all that was needed to rap off the new anchors/chains on top, but you could probably easily use a 60 meter cord.
5.12+ ffa rob pizem 2010
Props to the first ascensionist's for finding another cool pitch in the shade in the desert!
By david goldstein
May 7, 2015
In case you're wondering, the post-roof crux is about 30' of OW from #4 to #6 Camalot width, part of which seems overhanging.
Unless the leader back cleaned most of the gear in and below the roof, it would be hard to do this in one pitch due to rope drag and the rope running over/through the pieces in the roof.
The rap anchors are about 31 meters off the deck i.e. rapping with a single 60 might or might not work depending on how long your 60 meter rope is.
The summit is at the opposite end of the butte and is mostly an easy walk except for the 15' high summit knob itself which starts with an exposed, unprotected 5.6ish mantel on which most people would want to be belayed -- best not to leave your rope back at the anchors.
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Coronado Performing Arts Center - Menopause The Musical
Come join our sisterhood! This hilarious musical parody set to classic tunes from the '60s, '70s and '80s will have you cheering and dancing in the aisles! See what millions of women worldwide have been laughing about for over 10 years! It's the Hilarious Celebration of Women and The Change!®
Brian Regan Live at the Egyptian Theatre
Brian Regan has distinguished himself as one of the premier comedians in the country. Brian's non-stop theater tour has visited more than 80 cities each year since 2005 and continues through 2015. It is the quality of his material, relatable to a wide audience and revered by his peers, which continues to grow Brian's fan base. The perfect balance of sophisticated writing and physicality, Brian Regan consistently fills theaters nationwide with fervent fans that span generations. Presented by: Live Nation. Reserved Tickets $43.00. Tickets are on sale now!
Oh Ho Ho Holy Night
An original Christmas comedy musical, featuring songs from some of your favorite Christmas carols. F-Sa 7:30PM; Su 2PM.
CITY:Orland Park
Comedy Improv & Open Mic Night
Kidz On The Bridge & Graffiti On The Fourth Wall comedy improv troupes will perform, then Open Mic with singers, comics, magicians, dancers and more.
CITY:Tinley Park
VoicePlay at the James Lumber Center
VoicePlay recreates the orchestrated sound of an entire musical production with nothing but the human voice. The cast takes you between eras and styles, channeling the breadth of the musical landscape and lacing it with inventive and often hilarious on-stage theatrics.
How To Succeed In Business Without Really Trying at the Marriott Theatre
This Pulitzer Prize-Winning classic will follow the journey and the rise of eager young J. Pierrepont Finch. Finch, a window-washer joins a huge corporation by starting in the mail room, and becomes chairman of the board two weeks later. This show is one not to be missed.
Singin' in the Rain at the Marriott Theatre
Singin' in The Rain is based on the 1952 American musical comedy film. It offers a lighthearted depiction of Hollywood in the late '20s, with the three stars portraying performers caught up in the transition from silent films to talkies. This play will offer the audience some splashy old-fashioned fun.
The Peking Acrobats 30th Anniversary Tour at the James Lumber Center
The Peking Acrobats continue to entertain audiences of all ages with their daring maneuvers atop a precarious pagoda of chairs, tick-cycling, precision tumbling and gymnastics. They are guaranteed to keep audiences laughing and smiling as they sit on the edge of their seats!
America's Got Talent Live: The All-Stars Tour!
Buy tickets now and come see the All Stars of the hit show, America's Got Talent. This show comes with a variety of talent including comedian Taylor Williamson, Emily West the musical maverick, the acrobatics KriStef Brothers and Recycled Percussion. Tickets $32-$65.
Jenny McCarthy at the Genesee Theatre
Jenny McCarthy's Dirty Sexy Funny highlights the scandalous comedy of an eclectic cast of female comedians. Comedians Tammy Pescatelli, Lynne Koplitz, Paula Bel and April Macie will perform. This mixture of hilarious women shed much-needed light on what it's really like to be a woman. The audience will laugh until they cry as they dive into the raw, embarrassing and outrageously funny female facts of life. This is not your typical comedy show!
Sister Act at the Marriott Theater
Kicking off the 2016 Season, Sister Act will be a show not to be missed. This divine musical comedy is based on the hit 1992 film. Follow Deloris Van Cartier/Sister Mary Clarence through her journey while taking refuge in a convent.
The Egyptian Theatre Presents: The Dean Martin & Jerry Lewis Tribute Show
Dean Martin and Jerry Lewis were the hottest act in America during the early 50's. Dean of course, the straight man, with a charming voice and Jerry the lovable goof ball added the comedy. Never have two artists captured the look, the sound, the very essence and chemistry of these two "Icons" more than Tom Stevens and Tony Lewis. Both of these incredible performers have each had amazing success on their own. Tony spent the last 27 years performing in Australia and created a name for himself as the quintessential "Jerry Lewis Tribute Artist" selling out countless venues and earned respect as one of Australia's best entertainers. Since his 1st appearance at the annual Dean Martin Festival in 1998, Tom Stevens has earned his title as "Best Dean Martin Tribute Artist." Even Dean's daughter, Deana said, "I've never seen anyone look and sound more like my Daddy!"
18th Annual Fear No Art Festival at CLC
A festival like no other, the 18th Annual Fear No Art festival is a lively celebration featuring a variety of artists performing music, dance and poetry. Adding to the performances is the stunning display of paintings, photography and sculptures by CLC students and community artists.
Men are from Mars, Women are from Venus Live at the Effingham Performance Center
Based on the best-selling book, this hysterical show will have couples elbowing each other all evening as they see themselves on stage. The show starts at 8 p.m.
Whose Live Anyway
Whose Live Anyway is 90 minutes of hilarious improvised comedy and song all based on audience suggestions. Ryan Stiles, Greg Proops, Jeff B. Davis and Joel Murray, (Yes, one of the Murray brothers), leave their audiences gasping with the very witty scenes they invent before your eyes. Everyone is amazed at how quickly they make it up and crowds of all ages are astounded. Audience participation is the key to the show so bring your suggestions and you might be asked to join the cast onstage. Whose Live Anyway performs some of the games made famous from the Emmy Nominated TV show Whose Line Is It Anyway plus some new ones.
America's Got Talent at Genesee Theatre
This is the America's Got Talent All Stars Tour. The show will feature some of the most popular performers from the hit NBC variety competition series, including Season 8 favorite comedian Taylor Williamson. The #1 rated summer television series for the last ten years is coming to the Genesee and bringing the ultimate variety show.
CIC Theater
CiC is a non-profit comedy theater in Chicago featuring improv, sketch, and theatricals. Also a full service bar.
Last Comic Standing Live Tour at the Effingham Performance Center
Season 9 of Last Comic Standing premiers July 22 on NBC. The Emmy-nominated laugh-fest returns with an all new group of the world's funniest comics. Watch the series this summer on NBC, then see the finalist perform live as the Last Comic Standing Live Tour appears at the EPC! See Last Comic Standing Season 9 finalists perform live at 8 p.m.
Dave Rudolf's Halloween Concert for Kids at the Effingham Performance Center
Dave Rudolf serves up his special brand of Halloween entertainment through parodies, bubbles, hula hoops and audience interaction at 3 p.m. Costumes welcome!
Voca People at the Rialto Square Theatre
"Voca People" is a thrilling musical adventure unlike anything you experienced before! Full of energy and bursting with comedy and fun, it features more than 80 all-time favorite hits including Madonna, Michael Jackson and even Mozart! No instruments, no sound effects - just eight incredible, talented singer-comedians. No better venue than historic Rialto Square theater. 8:00pm show. .
Jeff Tracta at Batavia Fine Arts Centre
Jeff Tracta has become the most sought-after impressionist in the world today. With an array of over 100 spot-on impressions, Jeff pays tribute to over fifty years of music, comedy, and pop culture.From the most current music artists — the Black Eyed Peas, Eminem, and Lady Gaga — to legendary icons such as Bob Dylan, Billy Joel, and Elton John — and comedy tributes including Adam Sandler, George Lopez, Joan Rivers, Ray Romano, Chris Rock, Jeff Foxworthy, and the entire cast of Family Guy — Jeff's show has something for everyone.
Junie B. Jones' Essential Survival Guide to School at the Effingham Performance Center
Come see an all-new musical adventure based on Barbara Park's popular book at 10 a.m.
Sinbad at the Rialto
Ranked by Comedy Central as one of the top 100 standup comedians of all time, Sinbad has built a loyal following by taking day-by-day life and causing an uproar of comedic hysteria.
3 Redneck Tenors at the Effingham Performance Center
Get ready for a sidesplitting ride as we go on an excursion only the 3 Redneck Tenors could have had, delighting audiences with their vocal prowess and a smorgasbord of songs and music. See them live at the EPC at 7 p.m.


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Kaps Place
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San Jose, Costa Rica
Arrived in a bit of chaos, unsure of exactly where we were going or who to trust. The taxi ride to kaps place was a little hairy with the Costa Ricans not sticking to the lane system. Interesting contrast between main Tarmac 'motorway', corrugated iron/tin roofed shacks and huge holiday inn style hotels against a volcanic backdrop and rainforest …
Our last days in Costa Rica
... from Soda La Hormiga, where we ate 3869875673495 times. It's always white rice and black beans with a salad on the side and some sort of meat/pescado. This place always gave you a tortilla and an additional side, like spaghetti, vegetables, or...whatever the hell that is. Archie said it tasted like meat stuffing. I skipped that one. lol
Speaking of casados, we've decided that we're sick of rice and beans. The day before yesterday, we went to a little ...
La ciudad de San Jose
... volunteer work? Haha not really sure what it is but I will find out! Thankful for laughter and some quality team bonding tonight :) and that I took some steps out in faith today as I (attempted) to talk to some students in Spanish. Chao, Katherine "He will be the sure foundation for your times, a rich state of salvation and wisdom and knowledge; the fear of The Lord is the key to this treasure." Isaiah ...
First stop San Jose
... coffee, all very simple but the coffee and the bread were excellent. Out for a walk around town, to be honest, it's not the prettiest city we've visited, but after an hour or so it begins to wake up, lots of open markets a folk selling their wears on the side of the street, mostly fruit and the odd guy hawking cigars, we find the bus station we're looking for and book the ten AM bus to Puerto Viego for tomorrow morning. Spend an hour or two wandering around the city before ...


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Watch highlights from WME's "Arts of War" concert
The new my.harvard student information system is revitalizing the way students, faculty, advisors, and staff interact with the University.
The GSAS Fellowships Office announces a series of events for fall 2015 to help with fellowship applications and other forms of professional development, organized by Cynthia Verba, director of fellowships.
Save the Date for the Bok Center Fall Teaching Conference
Upcoming Events at the Office of Career Services
A physician and anthropologist who advanced global health now leads World Bank Group efforts to end extreme poverty.
Copyright © 1999-2015 by The President and Fellows of Harvard College


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Last Plan Standing
When different agendas come into conflict, it's a Foregone Conclusion in most cases that one and only one will emerge triumphant. When this happens, the expected result is that it's one of the major chessmasters who comes out on top (especially one who turns out to have been playing all the other players to their own ends), but it's just as likely that the final winner is a minor player, a Spanner in the Works who unknowingly sabotages the efforts of the would-be masterminds, or even someone who's simply lucky enough to be alive when the dust finally settles. In many cases, the Last Plan Standing is nothing of the sort - the winner is simply someone who happened to be in the right place in the right time and seized victory while all the other players had their attention elsewhere.
- Happens in every Pirates of the Caribbean movie to date, but especially Dead Man's Chest, with Norrington emerging as the surprise victor.
- On Lost, when the dust finally settles after all the years of scheming and back stabbing between Jack, Locke, Ben, Widmore, Jacob, and the Smoke Monster, it is kind-hearted Hurley who comes to rule the island.
- Simon at the end of Tad Williams' Memory, Sorrow and Thorn trilogy is at least a partial example.
- Fortinbras in Hamlet is established as seeking revenge on Denmark for past grievances. He ends up having the crown fall into his lap because all the legitimate claimants have killed each other.
- Liquid Snake at the end of Metal Gear Solid 2. His only real involvement with the plan was getting Solid Snake involved so that he was able to be released. However, the enormous circle of backstabbing between Dead Cell, Solidus, Olga and Ocelot cancelled them out to the point he was able to abscond with RAY. On a bigger scale, the Patriots managed to get exactly what they wanted out of the situation, successfully testing both their mind control system and eliminating one of their major enemies. And in Metal Gear Solid 4, Big Boss returns from the dead to effectively reveal himself as the Last Plan Standing for the entire series - the entire events of MGS2 and MGS4 were engineered to free the world from the grip of the Patriots.
- By the end of Legacy of Kain: Defiance, Raziel finally stops being manipulated by everyone, and lets himself be absorbed by the Reaver, so that Kain can use the Reaver to defeat the Elder God.
- In Adam Cadre's Varicella, the Villain Protagonist is tasked with manipulating a complex web of court intrigue so that he becomes the last and only choice for Regent. It backfires spectacularly when the young prince, who has become evil as a result of his upbringing, ascends the throne, eliminates the last obstacles in his path - including Varicella himself! - and declares war on everybody.
- In Final Fantasy Tactics, Delita marries Princess Ovelia and is crowned King Delita. However, this example is actually subverted. Delita is stabbed by Ovelia, and stabs her in return. Even if he does survive, he has lost everything that he was fighting for in his quest for power, and hundreds of years later, the revelation of the Durai Papers vindicates Ramza as the real hero of the game of the war.
- Of the many agendas from Dealer in Mega Man Star Force 3, only one really comes to fruition, and that's Heartless betraying them and using Meteor G as a means to contact Kelvin.
- Arabia and the Middle East were fought over by the Ottoman Empire, Germany, Britain and France, who were aided by the Rashidis (allied with the Ottomans), the Hashemites (allied with Britain), and the Saudis (led by Abdul Aziz Ibn Saud), along with others such as the Bedouins and the Ikhwan. As the region (plus a good deal more of the area) is now known as Saudi Arabia, it's clear who won. The Hashemites came in second, holding much of Mesopotamia and remaining in power in Jordan today. Most of the rest of the regional powers were devastated and replaced by entirely new states.
- Also happened in 1917-1921 Russia, where Czarists, Liberals, Anarchists, several Western Powers, Poles, and even a Czech Legion (prisoners liberated from Siberia) intermingled. Who would have bet on the tiny Bolshevik faction back in 1917?
- The United States might have been this after the Gambit Pileup that was World War I, as all the other major powers were devastated by war and falling apart. Instead, they went back to isolationism.
- Henry VII of England was this, both in Real Life and in William Shakespeare's play Richard III.
- Actually he doesn't come across as this in the play, which does a Historical Hero Upgrade on him, portraying him as the rightful King. Also many people forget that other Yorkists attempted this on him.


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I Heart My European Hometown
We asked our readers if the postcard was obsolete. The response was astounding. Seems the postcard isn't the dying art we feared it was. Hundreds of rectangle-shaped missives have flooded into Nat Geo headquarters to put a point on that fact. Some were sent by travelers reporting in from the road. But the lion's share were sent by people who just plain love where they live. Here's a dash of travel inspiration from hometown-proud locals in cities across Europe.
I Heart My Hometown: #PostcardProject
National Geographic Traveler features editor Amy Alipio recently asked our readers to weigh in on whether they thought the postcard was obsolete. The response was astounding. Seems the postcard isn't the dying art (and travel tradition) we feared it was. In the past three months (and with a little help from the Postcrossing.com community), hundreds of rectangle-shaped missives—from Shanghai to Sheboygan—have flooded into Nat…
@NatGeoTravel Staff Picks: Summer Festivals
In the mood to celebrate among the masses? There's nothing quite like an outdoor festival to make the summer season seem official. Here are a few of the @NatGeoTravel team's favorite open-air festivals to inspire your next trip.
Nat Geo Staff Picks: Favorite Travel Books
Travel literature. What is it? Memoirs detailing a life-changing trip to the Amazon, fiction that makes its setting a major character, nonfiction that asks its readers to consider a familiar destination in a new light—all of these fall under the travel literature umbrella. And personal preferences are just as varied. Here are a few favorites from Nat Geo Travel staffers.
The Best of Intelligent Travel
We publish new articles and stories all the time on Intelligent Travel, but there are a few that really got your attention last year. In case you missed them, here are the 14 most popular posts of 2014.
@NatGeoTravel Staff Bucket List: 2015
The staff at National Geographic Travel is continually criss-crossing the globe to uncover the best and the brightest places, but we have travel wish lists just like everyone else. Here's where we want to go in 2015 and why.
Oh, the Places Nat Geo Goes
When you work at National Geographic, one of the first questions people ask is if you get to travel. The answer is often yes, but one of the best parts of the job is being surrounded by sharp, globe-trotting people, and getting to hear their stories. That's why we asked folks on the Nat Geo Travel team to share a story about the best trip they've taken in the past year with our readers.
The Changing Face of Travel
Traveler's 30-year history coincides, roughly, with the rise of travel as a widespread phenomenon. As we celebrate the magazine's anniversary, I asked a dozen movers and shakers in the Nat Geo Travel family to share the biggest changes they've seen in the past three decades—and their hopes for the future. Here's what they had to say.
@NatGeoTravel Staff Picks: Best Fall Trips
With the changing of the seasons comes a change in where we as travelers set our sights on going. Inspired by our latest list of best fall trips, Nat Geo Travel staffers shared their own favorite autumn escapes. Here's a dozen to get you dreaming about your next adventure, near or far.
@NatGeoTravel Staff Picks: Best Islands
On the lookout for a great escape? There's nothing quite like an island to transport you to an alternate reality—one where days seem to stretch on forever and troubles fall away like an ebbing tide. Here are a few of the @NatGeoTravel team's favorite islands to get you in that dreaming mood.
Travel Lens: Amy Toensing's World
Amy Toensing, an American photojournalist committed to telling stories with sensitivity and depth, is known for her intimate essays about the lives of ordinary people. A regular contributor to National Geographic and National Geographic Traveler magazines, Toensing's assignments have taken her all around the world, from the Jersey Shore to the remote jungles of Papua New Guinea. Here's a look at the world through this award-winning photographer's unique lens.
Nat Geo Staff Picks: America's Best Beach Towns
Is there a magic formula for the perfect beach town? No, but America could offer up more than a few candidates if they were doling out the title. Here are just a few of them, recommended by Nat Geo Travel staffers.


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Thomas Hardy
Hardy's use of point of view is conventional for his time in literary history. It can be described as a third-person or omniscient point of view, which means that events can be viewed through the eyes of whatever character at the moment suits the author's purposes. Usually, though not always, such a person is one of the main characters, although within Hardy's lifetime, F. Scott Fitzgerald made a minor character the principal point of view in The Great Gatsby. Such a point of view is normally confusing to the reader only when it is shifted frequently in a short space, as, for example, in the melodramatic scenes in chapters 7-9 of Book Fifth.
Following the example of Henry James, a contemporary of Hardy's, modern novelists ordinarily use a more restricted point of view, in part because of the greater reality it lends to a work of fiction. Hardy himself was no innovator of fictional techniques.
Theme of The Return of the Native
Setting of The Return of the Native
Pop Quiz!


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Jonny Lee Miller
ELEMENTARY stars Jonny Lee Miller as detective Sherlock Holmes and... News PhotoArts Culture and Entertainment,Celebrities,Cracked,Crime,Detective,Elementary - Television Show,Fantasy,Jonny Lee Miller,Lucy Liu,New York City Police Department,Sherlock Holmes,Solution,Television Show,USA,VerticalPhotographer CBS Photo ArchiveCollection: CBS 2012 CBS Photo ArchiveNEW YORK - APRIL 3: ELEMENTARY stars Jonny Lee Miller as detective Sherlock Holmes and Lucy Liu as Dr. Joan Watson in a modern-day drama about a crime solving duo that cracks the NYPD's most impossible cases. ELEMENTARY premieres Fall 2012, Thursdays, (10:00-11:00 PM ET/PT) on the CBS Television Network. ( Photo by Craig Blankenhorn/CBS via Getty Images)


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Page 1
Molecular Biology of the Cell
Vol. 18, 1839–1849, May 2007
Kif5B and Kifc1 Interact and Are Required for Motility
and Fission of Early Endocytic Vesicles in Mouse Liver□
Sangeeta Nath,*†Eustratios Bananis,*†Souvik Sarkar,*†Richard J. Stockert,*
Ann O. Sperry,‡John W. Murray,*†and Allan W. Wolkoff*†
†Department of Anatomy and Structural Biology and *Marion Bessin Liver Research Center, Albert Einstein
College of Medicine, Bronx, NY 10461; and‡Department of Anatomy and Cell Biology, Brody School of
Medicine at East Carolina University, Greenville, NC 27858
Submitted June 19, 2006; Revised February 23, 2007; Accepted March 1, 2007
Monitoring Editor: Erika Holzbaur
Early endocytic vesicles loaded with Texas Red asialoorosomucoid were prepared from mouse liver. These vesicles bound
to microtubules in vitro, and upon ATP addition, they moved bidirectionally, frequently undergoing fission into two
daughter vesicles. There was no effect of vanadate (inhibitor of dynein) on motility, whereas 5?-adenylylimido-diphos-
phate (kinesin inhibitor) was highly inhibitory. Studies with specific antibodies confirmed that dynein was not associated
with these vesicles and that Kif5B and the minus-end kinesin Kifc1 mediated their plus- and minus-end motility,
respectively. More than 90% of vesicles associated with Kifc1 also contained Kif5B, and inhibition of Kifc1 with antibody
resulted in enhancement of plus-end–directed motility. There was reduced vesicle fission when either Kifc1 or Kif5B
activity was inhibited by antibody, indicating that the opposing forces resulting from activity of both motors are required
for fission to occur. Immunoprecipitation of native Kif5B by FLAG antibody after expression of FLAG-Kifc1 in 293T cells
indicates that these two motors can interact with each other. Whether they interact directly or through a complex of
potential regulatory proteins will need to be clarified in future studies. However, the present study shows that coordinated
activity of these kinesins is essential for motility and processing of early endocytic vesicles.
Receptor-mediated endocytosis is a process in which ligands
bind to specific cell surface receptors and internalize via
clathrin-coated pits. After internalization the clathrin coat is
released and uncoated vesicles mature into early endosomes
(Mellman, 1996; Mukherjee et al., 1997; Marsh and McMahon,
1999; Higgins and McMahon, 2002; Perrais and Merrifield,
2005). After acidification of early endosomes, ligands such as
asialoorosomucoid (ASOR) that are destined for lysosomes
dissociate from their receptors (Harford et al., 1983a,b), and
a series of fission events results in their segregation into
separate daughter vesicles (Wolkoff et al., 1984; Mellman,
1996; Mukherjee et al., 1997; Murray and Wolkoff, 2003). The
resulting ligand-enriched late endosomes traffic to lyso-
somes for degradation, whereas the receptor-containing
daughter endosomes traffic back to the cell surface where
receptor is reused (Harford et al., 1983a; Wolkoff et al., 1984;
Mukherjee et al., 1997). Previous studies indicated that this
segregation event requires an intact microtubule cytoskele-
ton (Goltz et al., 1992; Novikoff et al., 1996; Murray et al.,
2000; Bananis et al., 2003, 2004). In more recent studies,
microtubule-based endosome motility and segregation were
reconstituted in vitro using fluorescent early endosomes
prepared from rat liver 5 min after portal venous injection of
Texas Red-labeled ASOR, a substrate for the hepatocyte-
specific asialoglycoprotein receptor (ASGPR) (Bananis et al.,
2000, 2003, 2004; Murray et al., 2000; Murray and Wolkoff,
2003). On addition of ATP to the in vitro assay, these vesicles
moved bidirectionally along microtubules.
This in vitro system has permitted identification of native
endogenous proteins that are required for microtubule-
based processing of endocytic vesicles. Using these tools, we
found that plus-end motility of early endocytic vesicles from
rat liver was mediated by the conventional kinesin Kif5B
(standardized nomenclature kinesin-1) (Miki et al., 2003,
2005; Lawrence et al., 2004), and minus-end motility was
mediated by Kifc2 (standardized nomenclature kinesin-14B)
(Bananis et al., 2000, 2003, 2004; Murray et al., 2000). Whereas
Kifc2 was initially described as a brain-specific minus-end–
directed kinesin (Hanlon et al., 1997; Saito et al., 1997), our
studies showed that it was highly associated with these
vesicles prepared from rat liver (Bananis et al., 2003). Al-
though the rat is a convenient experimental animal, the
power of genetic models that have been established in the
mouse led us, in the present study, to examine microtubule-
based motility and processing of early endocytic vesicles
derived from mouse liver. Although, based on rat studies,
we expected to see altered endocytic processing of ASOR by
Kifc2 knockout mice (Yang et al., 2001a), we found no dif-
ferences as compared with wild-type mice. This provided
the rationale to define motors that mediate early endocytic
vesicle motility in mouse liver and to investigate interactions
of these vesicle-associated motors with each other. Such
interactions may be of great importance in coordinating
This article was published online ahead of print in MBC in Press
on March 14, 2007.
VThe online version of this article contains supplemental material
at MBC Online (http://www.molbiolcell.org).
Address correspondence to: Allan W. Wolkoff (wolkoff@aecom.
Abbreviations used: AMP-PNP, 5?-adenylylimido-diphosphate;
ASGPR, asialoglycoprotein receptor; ASOR, asialoorosomucoid.
© 2007 by The American Society for Cell Biology1839
Page 2
activities of opposing motors to regulate endosome fission
events as well as trafficking of vesicles to specific destina-
tions within the cell.
Chemicals and Reagents
ASOR was prepared from human orosomucoid (Sigma-Aldrich, St. Louis,
MO) by acid hydrolysis (Stockert et al., 1980) and labeled with Texas Red
(Murray et al., 2000) or125I (Wolkoff et al., 1984) as described previously.
Rabbit polyclonal antibody to the 17 N-terminal amino acids of the H1
subunit of the human ASGPR, which also cross-reacts with mouse ASGPR
(Treichel et al., 1994), and rabbit polyclonal antibody against Kifc1 (Zhang and
Sperry, 2004) were prepared as described previously. Rabbit polyclonal anti-
body against Kifc3 was purchased from Protein Tech Group (Chicago, IL).
Mouse monoclonal immunoglobulin G (IgG) against dynein intermediate
chain (IC) was purchased from Chemicon International (Temecula, CA).
Tubulin was purchased from Cytoskeleton (Denver, CO). Rabbit antiserum
against kinesin-1 (Kif5B) was provided by Dr. Lawrence S.B. Goldstein (Uni-
versity of California, San Diego, La Jolla, CA). Monoclonal antibody against
kinesin-1 heavy chain (H2) was generously provided by Dr. Scott Brady
(University of Illinois, Chicago, IL). Kinesin light chain (KLC) antibody was
purchased from Chemicon International. Cy2- and Cy5-labeled secondary
antibodies were purchased from Jackson ImmunoResearch Laboratories
(West Grove, PA) and Alexa 488-labeled secondary antibody was purchased
from Molecular Probes (Eugene, OR).125Iodine was purchased from GE
Healthcare (Little Chalfont, Buckinghamshire, United Kingdom). All other
reagents were from Sigma-Aldrich unless otherwise stated.
Wild-type C57BL/6J mice were purchased from The Jackson Laboratory (Bar
Harbor, ME). Kifc2 knockout mice were kindly provided by Dr. Lawrence S.B.
Goldstein. Male Sprague-Dawley rats (200–250 g) were purchased from Tac-
onic Farms (Germantown, NY). All animal procedures were approved by the
Animal Institute Committee of the Albert Einstein College of Medicine
(Bronx, NY).
Immunoblot Analysis
Immunoblots were performed as we have described previously (Bananis et al.,
2004). In brief, protein samples were subjected to SDS-polyacrylamide gel
electrophoresis (PAGE) under reducing conditions (100 mM dithiothreitol
[DTT]) and transferred to a polyvinylidene difluoride membrane (PerkinElmer
Life and Analytical Sciences, Boston, MA). The membrane was blocked with
Tris-buffered saline (TBS) (50 mM Tris-HCl and 150 mM NaCl, pH 7.6)
containing 0.1% Tween 20 and 10% nonfat dried milk before incubation with
primary antibody diluted appropriately in TBS, 0.1% Tween 20, and 2%
nonfat dried milk.
Preparation of FLAG-Kifc1 and FLAG-Kifc2 Expression
Kifc1 cDNA (Zhang and Sperry, 2004) was cloned into the pFLAG-CMV-5c
vector (Sigma-Aldrich) by using BamHI and HindIII restriction sites. Kifc2
cDNA (kindly provided by Dr. Lawrence S.B. Goldstein) was cloned into the
pFLAG-CMV-5a vector (Sigma-Aldrich) by using HindIII and EcoRV restric-
tion sites. Transient transfection of 293T cells with these plasmids was per-
formed using PolyFect transfection reagent (QIAGEN, Valencia, CA) accord-
ing to the manufacturer's instructions. Cells were harvested 2 d after
transfection, washed with phosphate-buffered saline (PBS), and lysates used
for further studies as described below.
Preparation of Brain Lysate
Mouse brain was homogenized in radioimmunoprecipitation assay buffer (50
mM Tris-Cl, pH 8.0, 1% NP-40, 0.1% SDS, 0.5% sodium deoxycholate, and 150
mM NaCl) containing 1:50 protease inhibitor (catalog no. P-8340; Sigma-
Aldrich). The homogenate was then subjected to centrifugation at 30,000 rpm
for 30 min in an SW60 rotor at 4°C. The supernatant was collected and stored
at ?80°C until use.
Preparation of Antibody to Kifc2
A peptide (GTPSSLSTDTPLTGTSC) containing 17 amino acids near the car-
boxy terminus (amino acids 746–762) of Kifc2 was synthesized by The Lab-
oratory for Macromolecular Analysis and Proteomics at the Albert Einstein
College of Medicine. This peptide was linked to maleimide activated keyhole
limpet hemocyanin (Pierce Chemical, Rockford, IL) according to the manu-
facturer's directions, and this was used for immunization of rabbits by Co-
vance Research Products (Danver, PA). Crude antiserum was purified on
SulfoLink gel (Pierce Chemical) to which the peptide was coupled according
to the manufacturer's protocol. Specificity of the antibody was assessed by
immunoblot of liver endocytic vesicles, brain lysate, and expressed FLAG-
Kifc2 (see below) in the presence and absence of peptide.
Isolation and Culture of Mouse Hepatocytes
Hepatocytes were isolated from mice after perfusion of the liver with colla-
genase type 1 (Worthington Biochemicals, Lakewood, NJ) (Xu et al., 1998).
Viability of cells was ?90% as judged by trypan blue exclusion. For studies of
125I-ASOR processing, 1.5 ? 106cells in Waymouth's 752/1 medium contain-
ing 25 mM HEPES, pH 7.2, 5% heat-inactivated fetal bovine serum, 26 mM
NaHCO3, 5 ?g/ml bovine insulin, 100 IU/ml penicillin, and 0.1 mg of
streptomycin were plated in each 60-mm culture dish (Primaria; BD Bio-
sciences, Franklin Lakes, NJ). The medium was changed after 2 h, and the
cells were maintained in culture for 16–18 h in a 5% CO2atmosphere at 37°C
(Wolkoff et al., 1984). For immunofluorescence studies, cells were cultured on
MatTek culture dishes (MatTek, Ashland, MA) coated with 0.5 mg/ml Matrigel
(BD Biosciences) using hepatozyme medium (Invitrogen, Carlsbad, CA).
Studies of125I-ASOR Internalization and Degradation by
Overnight Cultured Mouse Hepatocytes
Surface binding, internalization, and degradation of125I-ASOR by cultured
hepatocytes were assayed as described in previous studies (Wolkoff et al.,
1984; Samuelson et al., 1988). In brief, 1 ?g/ml125I-ASOR (6700 cpm/ng) was
added to hepatocyte monolayers in ice-cold binding medium (135 mM NaCl,
0.81 mM MgSO4, 1.2 mM MgCl2, 27.8 mM glucose, 2.5 mM CaCl2, and 25 mM
HEPES, pH 7.2) and incubated for 60 min at 4°C. Cells were washed four
times with ice-cold binding medium to remove unbound ASOR, and then the
surface-labeled cells were incubated at 37°C for 0–90 min. Ligand degrada-
tion was quantified as radioactivity remaining soluble after addition of an
equal volume of 20% trichloroacetic acid, 4% phosphotungstic acid to the
incubation medium. Cells were washed twice and surface binding was quan-
tified as radioactivity released following incubation in 20 mM EGTA in 0.15 M
NaCl, 0.02 M Tris-Cl, pH 7.6. Nonspecific binding was assessed by inclusion
of 100 ?g of unlabeled ASOR in the initial incubation with125I-ASOR.
Immunofluorescence Studies in Overnight Cultured Mouse
Cultured cells were exposed to 10 ?g/ml Texas Red ASOR for 5 min at 37°C
in hepatozyme medium, washed twice with warm PBS, and snap-frozen at
?80°C with a minimum of PBS to cover the cells. For immunostaining, cells
were thawed rapidly and fixed at room temperature for 15 min in 4%
formaldehyde at pH 7.4 in 0.25 M sucrose, 5 mM MgCl2, 5 mM EGTA, and 35
mM 1,4-piperazinediethanesulfonic acid (PIPES). They were washed with
PBS containing 5 mg/ml casein and incubated for 40 min in Kifc1 antibody
diluted 1:100 in this solution. After six washes with PBS containing 5 mg/ml
casein, cells were incubated for 40 min in Alexa 488-labeled secondary anti-
body diluted 1:1000. They were washed extensively and observed by fluores-
cence microscopy as described below.
Endosome Isolation and In Vitro Motility Assay
Texas Red-labeled early endocytic vesicles were prepared from mouse or rat
liver as described previously (Bananis et al., 2000, 2003; Murray et al., 2000). In
brief, livers were harvested 5 min after portal venous injection of 50 ?g of
Texas Red-labeled ASOR. A postnuclear supernatant was prepared after
Dounce homogenization of the liver and subjected to chromatography on a
Sephacryl S200 (GE Healthcare) column. Vesicle-enriched fractions were
pooled and centrifuged at 200,000 ? g for 135 min on a sucrose step gradient
consisting of 1.4, 1.2, and 0.25 M sucrose in a Beckman SW60 rotor. Vesicles
were collected from the 1.2 M/0.25 M sucrose interface and stored at ?80°C
until used. Motility assays were performed in a 3-?l chamber consisting of
two pieces of double-sided tape sandwiched between optical glass as de-
scribed previously (Murray et al., 2002). The chamber was coated with 0.03
mg/ml DEAE-dextran (GE Healthcare), and rhodamine-labeled, Taxol-stabi-
lized microtubules were added and incubated for 3 min at room temperature
(Bananis et al., 2000, 2003, 2004). The chamber was washed three times with
PMEE motility buffer (35 mM PIPES-K2, 5 mM MgCl2, 1 mM EGTA, 0.5 mM
EDTA, 4 mM DTT, 20 ?M Taxol, and 2 mg/ml BSA) containing 5 mg/ml
casein followed by three washes with PMEE motility buffer without casein.
Vesicles were then flowed into the chamber, incubated for 10 min to permit
binding to microtubules, and washed with PMEE motility buffer containing
10 mM ascorbic acid. Motility was initiated by the addition of 50 ?M ATP
without a regenerating system in the presence or absence of 5 ?M vanadate
or 1 mM 5?-adenylylimido-diphosphate (AMP-PNP). In some studies to quan-
tify directional motility, polarity-marked, rhodamine-labeled microtubules
were used. These polarity marked microtubules were prepared by first poly-
merizing seeds containing 10 mg/ml tubulin (1:75 labeled/unlabeled) in
polymerizing buffer (80 mM PIPES, 1 mM EGTA, 1 mM MgCl2, 1 mM GTP,
and 3% glycerol, pH 6.8) at 37°C for 5 min. The seeds were sheared by
pipetting up and down and subjected to extension at their ends after addition
of 2.5 mg/ml tubulin (1:6 labeled/unlabeled) for 6 min. The reaction was
stopped by adding polymerizing buffer containing 20 ?M Taxol. Microtu-
bules were pelleted by centrifugation for 4 min at 15 psi at room temperature
S. Nath et al.
Molecular Biology of the Cell1840
Page 3
in a Beckman airfuge and resuspended in polymerizing buffer containing 20
?M Taxol. In some experiments microtubule-bound vesicles were incubated
with antibodies against specific motor proteins for 6 min before the addition
of ATP (Bananis et al., 2003). In some immunofluorescence colocalization
studies, chambers were not coated with microtubules, and vesicles were
bound directly to the glass surface as we described in previous studies
(Bananis et al., 2000).
Immunofluorescence Studies of Vesicles In Vitro
Glass- or microtubule-bound endocytic vesicles were incubated with appro-
priately diluted primary antibodies in PMEE buffer (35 mM PIPES-K2, 5 mM
MgCl2, 1 mM EGTA, 0.5 mM EDTA, 20 ?M Taxol, and 2 mg/ml BSA)
containing 5 mg/ml casein for 6 min at room temperature, blocked with
motility buffer containing 5 mg/ml casein, and incubated with fluorescently
labeled affinity-purified secondary antibody for 5 min. For simultaneous
immunolocalization of two proteins, vesicles were first washed with motility
buffer containing 5 mg/ml casein after sequential incubation with the first set
of primary and fluorescent secondary antibodies. This was followed by incu-
bations with the second set of primary and contrasting fluorescent secondary
antibodies. The chambers were washed with PMEE motility buffer containing
10 mM ascorbic acid and examined by immunofluorescence microscopy
(Bananis et al., 2000, 2003, 2004).
Image Analysis
Imaging was performed with a 60? 1.4 numerical aperture Olympus objec-
tive on an Olympus 1 ? 71 inverted microscope maintained either at 28°C for
mouse vesicle studies or at 37°C for rat vesicle studies and containing auto-
mated excitation and emission filter wheels. Data were collected through a
CoolSNAP HQ cooled charge-coupled device (Photometrics, Roper Scientific,
Tucson, AZ) camera regulated by MetaMorph (Molecular Devices, Sunny-
vale, CA) software. Fluorescent images were analyzed using ImageJ (National
Institutes of Health public domain; http://rsb.info.nih.gov/ij/) and Adobe
Photoshop version 6.0 (Adobe Systems, San Jose, CA), and colocalizations
were scored manually. Colocalization of ASOR-containing vesicles with an-
tibodies to candidate proteins was quantified by first determining the number
of fluorescent vesicles in the rhodamine (ASOR) channel and then overlaying
the green channel to find the number of colocalized vesicles. For motility
studies, time-lapse movies were taken at 1 frame per second for 60 s. Movies
were analyzed using ImageJ software.
Interaction of Kif5B and FLAG-Kifc1
Preliminary studies showed that 293T cells have endogenous expression of
Kif5B (kinesin-1). 293T cells transiently transfected with pFLAG-Kifc1 or
pFLAG vector alone, as described above, were incubated for 60 min on ice in
immunoprecipitation buffer (50 mM Tris-HCl, pH 8.0, 150 mM NaCl, 50 mM
KCl, 10 mM EDTA, 10 mM EGTA, 1.5% Triton X100, and 0.75% NP-40)
containing protease inhibitors (catalog no. P-8340; Sigma-Aldrich). The lysate
was centrifuged at 14,000 rpm for 10 min at 4°C, and the supernatant was
incubated with nonimmune IgG-linked agarose (Sigma-Aldrich) for 3 h fol-
lowed by overnight incubation with anti-FLAG M2-linked agarose (Sigma-
Aldrich) according to the manufacturer's instructions. Immunoabsorbed pro-
tein was identified by immunoblot. In companion studies, 293T cells were
cotransfected with FLAG-Kifc1 and a pcDNA3 expression plasmid encoding
KLC2 (Ligon et al., 2004), kindly provided by Dr. Erika Holzbaur (University
of Pennsylvania). These cotransfected cells were processed as described above
to assess interaction of Kifc1 with KLC2.
Statistical Analysis
Statistical analysis was performed using chi-square or Student's t test as
Binding, Internalization, and Degradation of125I-ASOR
by Wild-Type and Kifc2 Knockout Mouse Hepatocytes
Based upon previous studies in rat early endocytic vesicles
showing that Kifc2 mediated their minus-end–directed mo-
tility on microtubules (Bananis et al., 2003), we hypothesized
that Kifc2 knockout mice would show a defect in endocytic
processing of ASOR. This was examined by quantifying
single-wave processing of125I-ASOR by overnight cultures
of hepatocytes isolated from livers of wild-type and knock-
out mice. In cells from both types of mouse, internalization
of cell surface bound125I-ASOR was rapid with ?70% in-
ternalized within 30 min of incubation at 37°C (Figure 1A).
Degradation of internalized ligand was nearly complete by
90 min in both wild-type and knockout cells (Figure 1B),
indicating that internalization and processing of125I-ASOR
in mouse liver do not require Kifc2, but likely use another
microtubule-based motor(s). This was examined in subse-
quent studies in vitro as described below.
Immunoblot Detection of Kifc2 in Mouse Liver
We showed previously that early endocytic vesicles prepared
and the plus-end kinesin Kif5B (Bananis et al., 2003). The pres-
ence of Kifc2 in vesicles prepared from mouse liver was as-
sayed by immunoblot. As seen in Figure 2, Kifc2 was detect-
able in rat liver vesicles and wild-type mouse brain lysate but
not in wild-type mouse liver vesicles. As expected, it was not
detectable in liver or brain from Kifc2 knockout mice. Immu-
noreactivity was removed by preabsorption of antiserum with
the peptide to which it was generated (Figure 2, bottom). These
results indicate that, as distinct from the rat, mouse liver early
endocytic vesicles are not associated with Kifc2.
Preparation of Early Endocytic Vesicles from Mouse Liver
To identify microtubule-based motors that are required for
motility, fluorescent early endocytic vesicles were prepared
Figure 1.
Kifc2 knockout mouse hepatocytes. (A) Binding of125I-ASOR to the
surface of hepatocytes was assayed as described in Materials and
Methods. The percentage of initially bound125I-ASOR remaining on
the surface of replicate plates at varied times after incubation at
37°C in hepatocytes from wild-type (closed circles) and Kifc2 knock-
out (open circles) mice is shown. (B) Release of degradation prod-
ucts of125I-ASOR into the medium was assayed as described in
Materials and Methods and is shown as percentage of initially bound
125I-ASOR. Each study was done in triplicate, and the error bar
represents SEM.
125I-ASOR binding and degradation in wild-type and
Mouse Early Endosome Motility
Vol. 18, May 2007 1841
Page 4
from mouse liver 5 min after portal venous injection of 50 ?g
of Texas Red ASOR. The procedure was identical to that
used for rat liver (Bananis et al., 2000, 2003; Murray et al.,
2000; Murray and Wolkoff, 2005). Vesicles were attached to
a glass motility chamber, and ASGPR was visualized by
immunofluorescence. Its distribution on vesicles was com-
pared with that of Texas Red ASOR. As illustrated in Figure
3, 95% of ASOR-containing vesicles isolated from both wild-
type and Kifc2 knockout mice were associated with the
ASGPR, indicating that these vesicles represent a population
of early (presegregation) endocytic vesicles similar to the
early endocytic vesicles that we described previously from
rat liver (Bananis et al., 2000).
Microtubule-based Motility and Fission of Mouse Liver
Early Endocytic Vesicles
These early endocytic vesicles from mouse liver were subjected
to time-lapse fluorescent microscopy to quantify their interac-
tion with and motility along microtubules. In contrast to pre-
vious studies using either rat endocytic vesicles (Bananis et al.,
2000) or vesicles containing herpes simplex virus (Lee et al.,
2006), when mouse early endocytic vesicles were assayed at
have been fixed to the slide began gliding, making the analysis
very difficult (data not shown). We found that this problem of
microtubule movement and detachment was markedly re-
duced when motility assays were conducted on mouse vesicles
at 28°C rather than at 37°C, and subsequent studies were
performed at this lower temperature. On addition of 50 ?M
ATP, 55% of the microtubule-bound wild-type mouse fluores-
cent early endocytic vesicles were motile (Figure 4A and Sup-
plemental Movie 1) with an average nonzero velocity of 0.60 ?
0.04 ?m/s (n ? 52, mean ? SEM). Among these motile vesicles
16% underwent fission (Figure 4B and Supplemental Movie 1).
When the vesicles were preincubated with 5 ?M vanadate, an
inhibitor of the minus-end–directed motor dynein (Kobayashi
et al., 1978; Bananis et al., 2000, 2004; Murray et al., 2000),
motility and fission were essentially unchanged (Figure 4, A
and B). However, pretreatment with 1 mM AMP-PNP, a kine-
sin inhibitor (Vale et al., 1992; Bananis et al., 2000, 2004; Murray
et al., 2000), before ATP addition inhibited vesicle motility and
fission completely (Figure 4, A and B). These results suggest
that microtubule-based motility of mouse early endocytic ves-
icles is mediated by kinesins and not dynein, similar to results
obtained with rat early endocytic vesicles (Bananis et al., 2000).
Early endocytic vesicles prepared from Kifc2 knockout mouse
liver behaved identically (data not shown).
Assessment of Directional Motility
The preceding studies examined total vesicle motility, but
they did not provide information regarding the direction
that vesicles moved on microtubules. To answer this ques-
tion, vesicle motility was assayed on directionally marked
microtubules. These studies showed that 50% of the motile
wild-type early endocytic vesicles moved toward the minus-
end, whereas 42% moved toward the plus-end of microtu-
bules (Figure 4C). The remainder of the vesicles (8%) moved
bidirectionally, indicating activity of both plus- and minus-
end motors on these vesicles. Early endocytic vesicles pre-
pared from Kifc2 knockout mouse liver behaved identically
(Supplemental Movie 2). These data show that early endo-
cytic vesicles prepared from wild-type or Kifc2 knockout
mouse liver move bidirectionally on microtubules and that
this movement is driven by plus- and minus-end–directed
kinesins. Because the vesicles from the knockout mice are
Figure 2.
formed after 7.5% SDS-PAGE of liver vesicles or brain lysate from
wild-type or Kifc2 knockout mice as indicated (15 ?g protein/lane).
Rat liver early endocytic vesicles and a lysate prepared from 293T
cells overexpressing FLAG-Kifc2 were used as positive controls.
Antiserum was used at 1:1000 dilution before (top) or after (bottom)
absorption for 3 h at room temperature with 10 ?g/ml peptide
against which it was made.
Immunoblot analysis of Kifc2. Immunoblot was per-
Figure 3.
wild-type (top) and Kifc2 knockout (bottom) mouse liver early endocytic vesicles were bound to rhodamine-labeled, Taxol-stabilized
microtubules and visualized in the rhodamine channel (left). Microtubule bound vesicles were incubated with antibody against the ASGPR
followed by Cy2-labeled secondary antibody and visualized in the fluorescein isothiocyanate (FITC) channel (middle). Yellow vesicles in the
merged figure (right) indicate colocalization of ligand (ASOR) and receptor (ASGPR). Bar, 10 ?m.
Colocalization of ASOR and its receptor in mouse liver early endocytic vesicles in vitro. Texas Red-labeled ASOR containing
S. Nath et al.
Molecular Biology of the Cell 1842
Page 5
devoid of Kifc2, the kinesin that mediates minus-end motil-
ity of rat early endocytic vesicles, we investigated whether
another minus-end kinesin was associated with these vesi-
cles. Aside from Kifc2, only four other minus-end kinesins,
Kifc1, -3, -4, and -5, have been described in mammals (Yang
et al., 1997, 2001b, 2006; Yang and Goldstein, 1998; Navolanic
and Sperry, 2000; Miki et al., 2001, 2003; Noda et al., 2001; Xu
et al., 2002; Yang and Sperry, 2003; Zhang and Sperry, 2004).
Kifc1 and Kifc3 were found to be associated with mem-
brane-bound intracellular organelles (Yang and Goldstein,
1998; Hoang et al., 1999; Noda et al., 2001; Xu et al., 2002;
Yang and Sperry, 2003; Zhang and Sperry, 2004; Yang et al.,
2006), whereas Kifc4 and Kifc5 are primarily active in mito-
sis (Yang et al., 1997; Zhang and Sperry, 2004). As seen in
Figure 5, immunoblot analysis showed that Kifc1 and Kifc3
were both present in liver and brain of wild-type and Kifc2
knockout mice. Further studies were then performed to
determine whether either of these motors was functionally
associated with early endocytic vesicles.
Immunofluorescence Colocalization of Motor Molecules
with Early Endocytic Vesicles
Using specific antibodies, we quantified the association of
the candidate minus-end–directed kinesins Kifc1 and Kifc3
with Texas Red ASOR-loaded early endocytic vesicles pre-
pared from wild-type and Kifc2 knockout mice. Kifc2 anti-
body was used as a control, because it did not detect the
protein on Western blot of mouse vesicle preparations. Cor-
responding studies were also performed using antibodies to
dynein and the plus-end–directed kinesin Kif5B. As seen in
Figure6A, immunofluorescence
showed that only antibodies to Kifc1 and Kif5B colocalized
with these vesicles. The relatively low degree of colocaliza-
tion of antibodies to dynein, Kifc2, and Kifc3 was similar to
that seen with nonimmune IgG (Figure 6A). Representative
immunofluorescence images showing colocalization of Kifc1
with wild-type and Kifc2 knockout mouse early endocytic
vesicles are seen in Figure 6B. To confirm that Kifc1 is
associated with endocytic vesicles in vivo, overnight cul-
tured mouse hepatocytes were incubated for 5 min at 37°C
with 10 ?g/ml Texas Red ASOR to label early endocytic
vesicles. Cells were then permeabilized by freeze-thaw,
fixed, and incubated with antibody to Kifc1 and Alexa 488-
labeled secondary antibody. As seen in Figure 6C, most of
the ASOR-containing vesicles in these cells were associated
with Kifc1.
microscopy analysis
Effects of Motor Antibodies on Mouse Liver Early
Endocytic Vesicle Motility and Fission
To assess which vesicle-associated motors mediated micro-
tubule-based motility, we quantified motility and fission in
Figure 4.
and directional motility of early endocytic vesicles from wild-type
mouse liver. Fluorescent early endocytic vesicles were bound to
microtubules. (A) Motility was initiated by addition of 50 ?M ATP
in the absence or presence of 5 ?M vanadate or 1 mM AMP-PNP.
The total number of vesicles counted is indicated above the bars.
*p ? 0.0005 compared with control, and values represent data
compiled from 10 (buffer) and four (vanadate and AMP-PNP) in-
dependent experiments. (B) The fraction of motile vesicles under-
going fission is shown. The total number of motile vesicles exam-
ined is indicated above the bars. (C) Polarity marked microtubules
were prepared and bound to the glass surface of the optical chamber
using DEAE dextran as described in Materials and Methods. Early
endocytic vesicles were then perfused into the chamber. The bars
indicate the percentage of motile vesicles moving toward the plus-
or minus-ends of the microtubules after addition of 50 ?M ATP.
Motility of 36 vesicles from five independent experiments was
examined in this representative study.
Effect of vanadate and AMP-PNP on motility, fission,
Figure 5.
were performed after 10% SDS-PAGE of wild-type mouse and Kifc2
knockout mouse brain lysates, rat liver early endocytic vesicles, and
wild-type or Kifc2 knockout mouse liver early endocytic vesicles (25
?g protein/lane) by using Kifc1 (top) or Kifc3 (bottom) antibodies.
Immunoblot analyses of Kifc1 and Kifc3. Immunoblots
Mouse Early Endosome Motility
Vol. 18, May 20071843


text:
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text:
This narrow hideaway in the heart of West Queen West supplies superior seafood in a friendly retro bar. The menu has few surprises, and not many options for...872 Queen Street W
A transplant from the East Coast, Rodney Clark imported his dad's oysters before opening his own joint. Rodney's is one of the city's first, and most...469 King Street W


text: We were driving back from Sturgeon Bay to Milwaukee early in the morning without anything to eat when we saw the sign to this restaurant. Thank God we did because the food was delicious,waitress was very friendly, and it wasn't expensive at all. It is definitely Luxemburg's own hidden gem.


text:
We're seeing a lot of memory makers incorporate the mighty Sandforce SF-1200 controller into their SSD line for higher read and write speeds, with the latest to do so being Patriot. The company has just announced its new Inferno series, and like other Sandforce-based SSDs, performance should be the least of your concerns (save your worries for the price).
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Available in 100GB and 200GB capacities, Patriot says its Inferno series will read data at up to 285MB/s and write files at up to 275MB/s, making them some of the fastest spec'd SSDs on the market. And to make installation easier, each Inferno drive ships with a 2.5-inch to 3.5-inch adapter, Patriot says.
Patriot didn't say anything about price or availability, but apparently they're already shipping. We found both drives on Newegg listed at $369 (100GB) and $679 (200GB).
Image Credit: Patriot


text:
« Jan. 9 Jan. 11 » Tuesday, January 10, 2012
San Francisco launches Golden Gate anniversary
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In brief: Interior: Oil rig near Cuba is safe
Jan. 10, 2012 in Nation/World on Page A3 HAVANA – A U.S. inspection of a Chinese-made oil rig due to begin drilling in waters off Cuba has determined that it meets international safety norms, the American government said …
Health spending slows
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Iranians sentence American to death
Jan. 10, 2012 in Nation/World on Page A3 WASHINGTON – The Obama administration Monday rejected Iran's charge that a young Iranian-American man used a family trip to Iran as cover for espionage, after the Tehran government issued the …
Daley leaves White House
Jan. 10, 2012 in Nation/World on Page A4 WASHINGTON – In a jolt to the White House, President Barack Obama announced Monday that chief of staff William Daley was quitting and heading home, capping a short and rocky …
Macy is now magnet thanks to 'Shameless'
Jan. 10, 2012 in City on Page A4 William H. Macy has earned critical acclaim for playing nerdy asexual characters in films such as "Fargo" and "Magnolia." But he says his role as a conniving deadbeat dad on …
Ruling overturns deputy's firing
Jan. 10, 2012 in City on Page A5 A Spokane County sheriff's deputy who was fired after he damaged a suspect's car and mishandled drugs from a crime scene has been rehired following a state arbitrator's ruling. Deputy … 30
In brief: Police probe baby's injuries
Jan. 10, 2012 in City on Page A5 Police are investigating after a 3-month-old Spokane girl was treated for broken bones, skull fractures and bruises Friday. Medics called police on Friday after treating the girl for injuries they …
State offers training on wolf kills
Jan. 10, 2012 in Idaho, Outdoors on Page A5 Washington state wildlife officials said they're willing to train Stevens County sheriff's deputies on how to identify livestock that has been killed by wolves. Washington Department of Fish and Wildlife … 1
Jim Kershner's This day in history » On the Web: spokesman.com/topics/local-history
Jan. 10, 2012 in City on Page A5 From our archives, 100 years ago An official of Spokane's city health department declared war on a serious threat to the morals of Spokane's boys and girls: public dance halls.
Sullivan should just bask in her one-hit glory
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Idaho records
Jan. 10, 2012 in City on Page A6 For 01-10-2012
Votes usher in new power
Jan. 10, 2012 in City on Page A6 There's a new majority in town. The Spokane City Council's new, more conservative majority flexed its muscle in its first meeting of the year on Monday when it voted 4-2 … 6
Avista outages zap customers
Jan. 10, 2012 in Business on Page A6 A theft of copper wire from an Avista Utilities substation early Monday morning caused a power outage for 5,800 Coeur d'Alene customers. And Monday afternoon a brief downtown Spokane power …
Inland Crafts, longtime show, shuts down
Jan. 10, 2012 in City on Page A6 Inland Crafts has folded after 29 years of hosting a juried fine-crafts exhibition and sale in Spokane. The nonprofit's board decided hosting the annual show was "more than we felt …
Washington records
Jan. 10, 2012 in City on Page A6 Spokane County Marriage licenses
Medical issues close Snake Pit
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Idaho top court upholds gang law
Jan. 10, 2012 in City on Page A6 BOISE – The Idaho Supreme Court has decided a state law designed to stop gangs from recruiting new members is constitutional. A divided court handed down the ruling late last … 1
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Borrowing surges amid flat income
Jan. 10, 2012 in Business on Page A7 WASHINGTON – Americans are feeling confident enough in the economy to go back to a time-honored tradition – taking on a little extra debt. Consumer borrowing surged in November by …
Novartis recall may also affect painkillers
Jan. 10, 2012 in Business on Page A7 WASHINGTON – The Food and Drug Administration is warning patients about a potential mix-up between powerful prescription pain drugs and common over-the-counter medications made at a Novartis manufacturing plant. The …
TVs you can talk to hit electronics show
Jan. 10, 2012 in Business on Page A7 LAS VEGAS – Talking to the TV is usually a sign of extreme agitation, mental instability or loneliness. TV manufacturers are set to make it a more rational behavior this …


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Suburbia Wall Storage
Written by Katie on April 4, 2014. Permalink
If you're in need of wall storage and haven't yet fallen in love with the Uten.Silo ($395), then maybe you'll like the Suburbia by Seletti. When viewed from its edge, a small neighborhood with fences, trees, and homes appears. That novel detail does well when hung vertically, as it provides plenty of nooks and crannies to store all sorts of things in the office, bathroom, or entryway. Design by Note ...Read more
Purchase Information:Price: $478.13
Available from: YLiving