text:
water distribution networks Companies in Maine
Stevens Water Monitoring Systems, Inc.
based in Portland, OREGON (USA)
Stevens Water Monitoring Systems, Inc.`s mission is to serve our customers worldwide and be our customers` single source for their water resource equipment needs. Stevens designs, manufactures and supports a broad range of instruments and accessories that provide water level, water quality, ground water, soil moisture, and weather information. These products are easy to ...
Modern Water - Monitoring Division
based in Cambridge, UNITED KINGDOM
Modern Water's Monitoring division has developed some of the world's most advanced systems for monitoring water quality. We provide market-leading toxicity monitoring systems, trace metal and environmental monitoring systems. The range includes systems for portable, online, and laboratory use. Our toxicity monitoring products, Microtox and Microtox CTM, detect toxins in ...
Commercial Water Distributing LLC
based in Zumbrota, MINNESOTA (USA)
Commercial Water Distributing LLC is a master distributor of the best brands in the water purification industry. Commercial Water Distributing has earned a reputation with its manufacturers as an aggressively growing company that follows through on its commitment of integrity and customer service.
The Training Network
based in Durham, NORTH CAROLINA (USA)
The Training Network (formerly known as Long Island Productions) produces and distributes safety training videos, safety training kits, and other safety training products to assist organizations with OSHA Compliance, workplace safety and Human Resource training issues.Founded in 1981, the Training Network offers competitive prices on over 900 safety training and human ...
Ovivo (Formerly Eimco Water Technologies)
based in Salt Lake City, UTAH (USA)
Following the merger of Eimco Water Technologies, Enviroquip and Christ Water Technology, September 2010 sees the emergence of a new global force in the water sector, dedicated to bringing the latest thinking, proven technologies and the most advanced application knowledge to the municipal and industrial clean water, process water and wastewater markets around the globe. ...
GE Power & Waters Distributed Power
based in West Chester, OHIO (USA)
GE Power & Water's Distributed Power portfolio of innovative solutions give businesses and communities around the world the ability to generate reliable and efficient power at or near the point of use, anytime – on the grid or off. Our product portfolio features Aeroderivative gas turbines, Jenbacher gas engines, and Waukesha gas engines ranging from 120 kW-100MW. ...
Adedge Water Technologies, LLC
based in Buford, GEORGIA (US) (USA)
Adedge Water Technologies, LLC specializes in the development and supply of innovative technologies, adsorbent-based products, and systems that remove contaminants from process or aqueous steams. We are an organization based on solid deliverable, scalable, scientifically proven technology and experience for the removal of arsenic and other contaminants from water. Our ...
Eijkelkamp Soil & Water
based in Giesbeek, NETHERLANDS
As part of Royal Eijkelkamp, Eijkelkamp Soil & Water contributes to the following mission and vision. Mission: Royal Eijkelkamp is able to make a continuous contribution to a safe and healthy living environment thanks to more than 100 years' experience and expertise in soil and water research. Vision: Royal Eijkelkamp wants to become the world's preferred supplier of ...
Seneca Economics and Environmental Management Network
based in Germantown, MARYLAND (USA)
The Seneca Economics and Environmental Management Network is a network of independent consultants who share an interest in solving environmentally-related challenges.1 Each of us has spent between fifteen and thirty years working on relevant issues, including time spent as employees of or consultants to various federal agencies, including the U.S. Environmental Protection ...
VAC-CON, Inc.
based in Green Cove Springs, FLORIDA (USA)
VAC-CON, Inc. manufactures premium, truck mounted combination sewer cleaners, catch basin cleaners, sewer flushers and hydroexcavators marketed and supported by a worldwide network of carefully chosen and well-trained distributors.


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July 21, 2014
KANSAS CITY, Mo. - Former Colorado State guard Josh Morgan was honored Monday as one of a record 895 student-athletes from 335 colleges and universities when the National Association of Basketball Coaches (NABC) announced its 2013-14 Honors Court.
The NABC Honors Court recognizes junior and senior men's basketball players at all levels of college basketball, who have spent at least one year at an institution and boast a cumulative grade-point average of 3.2 or higher.
Morgan, a 6-2, 185-pound product from Buena Vista, Colo., spent two seasons on the Colorado State men's basketball team after joining the team as a walk-on transfer from NCAA Division II Hawai'i-Hilo. Morgan, regarded as one of the best students on the squad, completed his degree from Colorado State in May, graduating with a degree in Health and Exercise Science.
He is the second player in as many seasons from Colorado State to earn a spot on the NABC Honors Court list, joining former Rams great Dorian Green who was named to the list as a senior in 2012-13.
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#Mmmonday: These spooky snacks are almost too cute to eat
I don't have a BF yet. Is something wrong with me?
My room is a disaster...HELP!
Have you ever witnessed something horrible...and not known what to do? This book is a must-read
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88 Comments | Add Yours
Ewww ok grl hes a security guard and an ADULT. No way on earth is it ever a good idea to like someone that is so much older then u. Go for some cutie at ur skool that doesn't creep u out please.
by fashionstar2be on 2/24/2010 1:55:53 AM
We all usually crush on an older guy especially guys we cant have like some actors and musicians and sometimes we even have crushes on teachers and other guys but you should be careful with this guy. Like I have a teacher who is very hot and I have had dreams about him and that creeped me out. So I think being creeped out is because you might be scared to have thoughts about an older guy, because I think that is what happened to me.
I love the idea about making a "Book of Guys" and I cant wait to do this with my friends at our next sleep over. I think I am going to come up with a list of hot guys we all crush on and we are going to grade them on those items and more. Most will be guys 2 or 3 years older but some might even be older but it will be fun to do. What an awesome idea, thanks GL!
But I also think its normal to crush on older guys because they are more mature, just like we are.
by luckykel on 2/24/2010 12:07:53 AM
by summernight_dreams on 2/23/2010 10:57:03 PM
And sounds like a good idea to have a "book". just for fun though, I dn't have a type. I"ma fill out that thing about my bf just to though! haha
1. Age/ Grade: He will be 16 next month (A year, one month and 4 days older than me
2. Height: Well he sure is taller..
3. Body Structure: He's... normal. Muscular.
4. Hair: His hair is usually in between.. I personally secretly like it longer.
5. Eye Color: *siiiggh* the most beautifulest shade of brown. With flecks of greenish..
6. Athlete: Yup. Cross Country, Football basketball.
7. Musician: He sings (really well), he wrote me a song. He plays guitar (yes.. yes yes!), and he is an actor!
8. Personality? (Funny/Shy/flirty): He's funny, slightly shy when you first meet him, he def flirts with me, we just flirt back in forth its like a huge game, but I've never seen him flirt with otha girls.
9. Interests: ME. Hehe I intrest him apparently
10. Hobbies: Acting.. and such.
by reeg-a-lene on 2/23/2010 10:20:44 PM
Great advice I see here.. And Its totally creepy.. Sometimes when a guy likes you (even if you really think about and he's really gross) you think you're crushing on him.. It just happens.. Its like you think you should lik ehim back just cuz he likes you.. But when you stop and truly seriously think about it, it all straightens out.
Check out my profile girls (I hate doing this but I haven't had comments in a while and I'd liked to talk to ya'll) I give advice, mostly on guys and esp flirting. And I love to just chat too.
by reeg-a-lene on 2/23/2010 10:13:33 PM
haha!! in my last comment i spelled "would" and "graduation" wrong!!!
im smart(:
by verenice<3 on 2/23/2010 10:10:41 PM
by verenice<3 on 2/23/2010 10:00:43 PM
by Cherries51 on 2/23/2010 9:48:46 PM
OMG thats disturbing!
For the love of god, please dont choose him!
sounds like a harasser to me. STAY AWAY!!!!
by gurdigirl123 on 2/23/2010 9:41:29 PM
stay away from him!!
how can you even like him?
by gurdigirl123 on 2/23/2010 9:40:25 PM
GIVEAWAY: Fab up your life with these *free* fall finds!
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'Mission: Impossible 5' Taps New Writer (Exclusive)
Will Staples will write the script for the fifth installment in the Tom Cruise action franchise, which is slated for release on Dec. 25, 2015.
Paramount, Skydance and Tom Cruise have tapped Will Staples to work on the script for the fifth Mission: Impossible.
Christopher McQuarrie is attached to direct the film, which will see the return of Cruise as Ethan Hunt. Paramount has slated it for release on Dec. 25, 2015.
PHOTOS: Buff Bods Over 40: 7 Actors With Ripped Physiques
Drew Pearce, who wrote Iron Man 3, previous worked on the script, but that was before McQuarrie signed on in August 2013.
Staples' previous work has been mostly in video games. He worked on Need for Speed: Rivals and Call of Duty: Modern Warfare 3. He's attached to several films in development, including Blood & Treasure, a starring and directing vehicle for Ben Affleck set up at Warner Bros. He also wrote Myth for Fox and the Jeremy Renner vehicle King of Heists. He's repped by WME, Management 360 and Bloom Hergott.
Tom Cruise Productions, Paramount and J.J. Abrams' Bad Robot are producing Mission: Impossible 5, and David Ellison's Skydance is co-producing.
The previous Mission Impossible: Ghost Protocol, released in December 2011, reinvigorated the franchise, grossing an outstanding $694.7 million worldwide.
Never miss a story. Stay up-to-date with THR's Daily Headlines.
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Hollywood's Notable Deaths of 2015


text:
The Seagate® Kinetic Open Storage platform is the first device-based storage platform enabling independent software vendors (ISV) and cloud service provider (CSP), and enterprise customers to optimize scale-out file and object-based storage, delivering lower TCO. Seagate Kinetic Storage comprises storage devices + key/value API + Ethernet connectivity.Learn How Seagate Kinetic HDD Is Revolutionizing Cloud Storage >
Rapid implementation and deployment in any stack.
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Get hands-on with the Kinetic API source code.
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The Seagate Kinetic Open Storage platform delivers higher performance and capacity, along with improved rack density, by allowing more flexibility than traditional storage server architectures.
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As part of the ATA's peer- and industry-selected award process, the Kinetic platform was judged based on innovation, intellectual property, commercial viability, value proposition and impact on the industry and economy.
"This is yet another proof point of market validation of the Kinetic vision and opportunity," said Ali Fenn, senior director, product line management, at Seagate. "We are thrilled to have won this honor against a field of very significant nominees."
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With the advent of platforms such as software-defined service (SDS), Web cloud, and high-performance computing (HPC), storage demands are becoming ever more dynamic and challenging. These storage environments not only require increased density and performance, but they also must be deployed inexpensively. When storing petabytes of information, for example, reducing costs by pennies per gigabyte […] ...
All storage is the same, right? Wrong! Some folks misuse storage, thinking all hard drives are the same. But when it comes to specialized applications, Seagate goes the extra mile to ensure your drive will perform with tough loads for the long haul — when you choose the right drive for your workload. Every hard drive, […] ...
Note: Sofocles Socratous, VP of EMEA sales and marketing for Seagate, wrote this guest column on storage for the surveillance market last week for Security News Desk, a news and product-information portal for the security industry. Market researcher Trendfocus estimates that hard drive manufacturers will ship approximately 20 million surveillance drives in 2019, up from […] ...
What if your Wi-Fi router at home could have a 6TB hard drive from Seagate on board? Imagine how that might make it easy to download, copy, keep and access all the stuff your family shares throughout the house! Of course, our Seagate Personal Cloud already offers a super sweet way to keep, store, backup and share your […] ...


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Egypt UsEgypt Us (1960) Episode 36- Popeye Cartoon Episode Guide
This page has been viewed 14 times this month, and 799 times total.
- 1960
- TV Spots
- King Features Syndicate
- Popeye Episode Guide
- Cartoon Characters: Popeye, Olive Oyl, Wimpy, Egyptians.
- Vocal Talent: Jack Mercer (Popeye, Wimpy), Mae Questel (Olive Oyl, Swee'Pea, Eugene).
- Directed By Tom McDonald.
- Produced By Gerald Ray.
Executive Producer: Al Brodax.
- Animated By Izzy Ellis, Sam Kai, Casey Onaitis, Ray Young, Bill Higgins, Barney Posner, John Garling, Bud Partch.
- Edited By Norm Vizents.
Additional Crew:
- Layout: Henry Lee.
- Background: Dave Weidman.
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Cartoon Summary:
Thinking that they are going to the beach at Coney Island, Popeye, Olive Oyl and Wimpy take a wrong turn en route (Olive reads the map wrong), and they end up in the Egyptian desert. The car quits. Popeye, Olive and Wimpy are walking across the desert in bathing... (more)
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Egypt Us
the bloated moocher... (read more)


text: Friends took us to Dahlak in West Philadelphia. The building itself is a bit worn and slightly run down. The restaurant is dark and looks like it could use a good facelift. Some of the tables are low round traditional tables made from wicker. They are a little low and look uncomfortable. You... read more


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Clark Ellis brings us Part 2 of an interview with Dr. Lucinda Bateman, where she answered questions posed by the patient community …
Discuss the article on the Forums.
Deteriorating handwriting - is this an ME symptom?
Discussion in 'Neurological/Neuro-sensory' started by Sasha, Jul 5, 2011.
Page 1 of 2
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Join OkCupid
Find better matches with our advanced
Find better matches with our advanced
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26 • Williamsburg, KY • Woman
I'm looking for
- Men
- Ages 23–27
- Near me
- Who are single
- For long-term dating, short-term dating
My details
- Last online
- Dec 14, 2012
- Orientation
- Straight
- Ethnicity
- White
- Height
- 5′ 6″ (1.68m)
- Body Type
- Average
- Diet
- Mostly anything
- Smokes
- No
- Drinks
- Socially
- Drugs
- Never
- Religion
- Christianity, and very serious about it
- Sign
- Capricorn, and it's fun to think about
- Job
- Other
- Income
- Rather not say
- Status
- Single
- Offspring
- Doesn't have kids
- Pets
- Likes dogs and likes cats
- Speaks
- English
My self-summary
Write a little about yourself. Just a paragraph will do.
im 23 years old and im looking for a good true relationship that that will be for a long time im really wanting to find the one person that will love me and me true to me i love the outside and camping and going on long walks and watching a good movie and playing video games and photography now that i am able to
What I'm doing with my life
Don't overthink this one; tell us what you're doing day-to-day.
right now i am helping take care of my dad since my mom died and trying to get a good person to be with and later on try to get on a nurseing home
I'm really good at
Go on, brag a little (or a lot). We won't judge.
cooking makeing candles being there when a friend needs somebody to talk to showing how much i care about the person im in a relationship with takeing care of old people
The first things people usually notice about me
I'm an empty essay… fill me out!
my smile my eyes my personality
Favorite books, movies, shows, music, and food
Help your potential matches find common interests.
i lkie a variety of music i lkie some classical and some 80s rock and i live makeing homemade pasta pizza bread bowls and taco salads i lkie the cake boss shows and walking dead the series the show tanked deals from the dark side some pop music and country
The six things I could never do without
Think outside the box. Sometimes the little things can say a lot.
my purse my phone some money on hand good friends
I spend a lot of time thinking about
Global warming, lunch, or your next vacation… it's all fair game.
the better of things how i can be a better person and how i can help my friends and family
On a typical Friday night I am
Netflix and takeout, or getting your party on — how do you let loose?
playing a video game or watching a good movie and hanging out with friends
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I'm an empty essay… fill me out!
im pretty much open but the most private would be i sill hold on to things that are from years ago i collect beanie babies and stuff that my friends dont
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Page 1
glmS riboswitch binding to the glucosamine-6-phosphate α-
anomer shifts the pKa toward neutrality
Jared H. Davis, Brian F. Dunican, and Scott A. Strobel*
Department of Molecular Biophysics and Biochemistry, Yale University, 260 Whitney Avenue
New Haven, Connecticut 06520-81114
The glmS riboswitch regulates gene expression through a self-cleavage activity. The reaction is
catalyzed with the assistance of the metabolite cofactor glucosamine-6-phosphate (GlcN6P),
whose amino group is proposed to serve as the general acid during the reaction. This reaction is
pH-dependent with a pKa that is lower than the observed pKa for the amine of GlcN6P in solution.
GlcN6P, like other pyranose sugars, undergoes spontaneous and rapid interconversion between the
α and β anomers at the C1 position. Here we demonstrate by NMR that the Bacillus anthracis
glmS riboswitch selectively binds the α-anomer of GlcN6P with a maximum binding affinity of
0.36 mM, and that binding is pH-dependent. We also report that the anomeric ratio between α and
β is pH-dependent and the pKas of the two amines differ by 0.5 pH units, α being the higher of the
two (pKa = 8.3). The pH dependence of binding reveals a pKa of 6.7, suggesting that the glmS
RNA reduces the pKa of the GlcN6P amine by 1.6 units in the ground state. We reevaluated
previously obtained kinetic data and found the reaction pKa is 6.9, within error of the binding data.
The data support a model where the reaction pKa corresponds to that of the GlcN6P amine. This
observation has broader relevance for considering how the microenvironment of an RNA, despite
its anionic character, can reduce the pKas of functional groups for use in catalysis.
The glmS gene codes for the enzyme glutamine-fructose-6-phosphate amidotransferase,
which produces glucosamine-6-phosphate (GlcN6P) from fructose-6-phosphate and
glutamine (1). GlcN6P is an essential metabolite that is processed to the activated form N-
acetylglucosamine and used to make peptidoglycans, lipopolysaccharides and teichoic acids
in bacteria; chitin in fungi, insects and crustaceans; and glycoproteins, glycosaminoglycans
and mucopolysacharides in mammals (1). Given the importance of GlcN6P, it is not
surprising that deletion of the glmS gene is lethal in bacteria, making this gene an attractive
antibiotic target (1–4).
In many Gram-positive bacteria, the glmS gene is regulated by a riboswitch that is found in
the 5' untranslated region of the gene (5). This glmS riboswitch senses the concentration of
GlcN6P and regulates the expression of the amidotransferase (5, 6). However, unlike the
majority of riboswitches described to date, the glmS riboswitch does not function by
stabilizing alternative structural conformations upon ligand binding. Instead, GlcN6P
functions as a metabolite cofactor that promotes self-cleavage of the glmS RNA (5, 7).
Cleavage destabilizes the downstream mRNA by generating a 5'-terminal OH that is
recognized as an mRNA degradation signal by ribonuclease J1 (6).
Biochemical and structural data have identified the overall mechanism of RNA strand
scission in this ribozyme. Upon GlcN6P binding, the glmS riboswitch is proposed to react
via a general acid-base mechanism with the amine of GlcN6P functioning as the general
Contact information Phone: 203-432-9772, Fax: 203-432-5767, firstname.lastname@example.com.
NIH Public Access
Author Manuscript
Biochemistry. Author manuscript; available in PMC 2012 August 23.
Published in final edited form as:
Biochemistry. 2011 August 23; 50(33): 7236–7242. doi:10.1021/bi200471c.
NIH-PA Author Manuscript
NIH-PA Author Manuscript
NIH-PA Author Manuscript
Page 2
acid (7–10). Crystal structures of the Bacillus anthracis and the Thermoanaerobacter
tengcongensis glmS ribozymes bound to GlcN6P have been reported and in each case the
amino group is in close proximity to the O5' leaving group (8, 10). The reaction is pH-
dependent with a pKa between 7.6 and 7.8 (7, 9, 11). Because this pKa is near the pKa of the
amine of GlcN6P in solution (8.2), it has been attributed to the pKa of the amine in the
transition state (7, 9). However, this would require a shift of ~0.5 pH units in the acidic
direction in the transition state. The observation that other ligands, including serinol and
glucosamine, with a primary amine can catalyze the reaction provided additional evidence
that the amine acts as the general acid (7). Furthermore it has been observed that the reaction
pKa for serinol and glucosamine match the solution pKa of the amine groups of these ligands
(7). Thus, it seems likely that the pKa of the GlcN6P amine is being lowered, at least in the
transition state.
Like all pyranose sugars, GlcN6P undergoes spontaneous isomerization at the anomeric
carbon (C1 position) to produce an α and a β-isomer (Figure 1) (12). Previous efforts to
interpret the glmS ribozyme reaction did not consider the role this isomerization might play,
or that the riboswitch may selectively bind and react with only one of the two anomers. A
previous attempt to determine if the reaction is preferentially catalyzed by one of the
anomers was inconclusive because neither of the GlcN6P analogs used to mimic the α or β
conformations was reactive (13). All structures of the glmS riboswitch have modeled
GlcN6P using the α-anomer. The high resolution T. tengcongensis structure (1.7Å
resolution) is at a sufficient resolution to be reasonably confident about such an assignment,
but even then, it would be difficult to identify fractional binding of the β-anomer (10).
Surprisingly, several aspects of GlcN6P isomerization chemistry are unknown. These
include the α/βisomeric ratio at equilibrium, the pH dependence of that ratio, the pKas of the
amines for the individual isomers, and the rate of GlcN6P interconversion. Furthermore, it is
unclear if the glmS RNA has a preference for one anomer over the other. It is possible that
the discrepancy between the solution pKa of GlcN6P and the reaction pKa is due to
anomeric specificity of the glmS riboswitch. Characterization of these parameters is
necessary to properly interpret the pH-dependent reaction kinetics of GlcN6P-induced glmS
RNA cleavage and to understand how the metabolite is used as a catalytic cofactor.
In the present study we have used NMR to determine the pKa of the amine of GlcN6P bound
to the glmS riboswitch. We also determined the anomer specificity of GlcN6P and the nature
of GlcN6P isomerization in the free form. We report that the glmS riboswitch specifically
binds to the α-anomer in a pH-dependent fashion, with a preference for the deprotonated
form and a pKa of 6.7. This is consistent with the active site of the glmS riboswitch shifting
the pKa of the amine in the acidic direction, which has implications for other RNA active
sites where general acid-base chemistry has been proposed (14–19).
Materials and Methods
Substrate preparation
The inactive glmS RNA with a 2' O-methyl substitution at the cleavage site was prepared as
a two-piece construct as previously described (8). The transcribed portion of the RNA was
purified from the HDV ribozyme and abortive products by size exclusion chromatography
using a Hi Load 26/60 Superdex™ 75 prep-grade column (GE Healthcare). The
oligonucleotide portion of the riboswitch was purchased from Thermo Scientific. Both
RNAs were purified using a G-25 Sephadex column to remove salts. The RNAs were
diluted to between 50 and 75 µM in 50 mM NaCl, 10 mM sodium acetate (pKa 4.8), 10 mM
triethanolamine (pKa 7.8), and 10 mM MgCl2 at the pH needed for the experiment. The D-
glucosamine-6-phosphate sodium salt (GlcN6P) and D-glucose-6-phosphate sodium salt
Davis et al. Page 2
Biochemistry. Author manuscript; available in PMC 2012 August 23.
NIH-PA Author Manuscript
NIH-PA Author Manuscript
NIH-PA Author Manuscript
Page 3
(Glc6P) was purchased from Sigma-Aldrich®. A 1 M solution of both sugars was prepared
in 50 mM NaCl, 10 mM sodium acetate (pKa 4.8) and 10 mM triethanolamine (pKa 7.8) at
the desired pH. All GlcN6P samples contained 10% D2O. Because the water peak
overlapped with the chemical shift of the H1 proton for the α–anomer, the Glc6P sample
was dried and resuspended in 100% D2O.
NMR spectroscopy
All NMR experiments were performed using a Varian 500 MHz spectrometer with a HCN
triple axis probe at 25° C running VNMR-J. Chemical shifts of the H2 and H1 protons were
obtained by the known chemical shifts and J-coupling of the two isomers of other glucose
sugars (12), and confirmed using NOESY data. All 1D data were analyzed using
MestReNova 6.6.1. 2D data were analyzed using Sparky 3.115.
1H NMR pH titration
NMR samples were titrated to the appropriate pH using NaOH. Ten 3 mM samples of
GlcN6P were prepared between pH 5.5 and 10.5 at intervals of 0.5 pH units. The pH was
tested before and after the NMR experiment. No significant change in pH was observed over
the course of the experiment. 1D 1H spectra with water suppression were collected at each
pH. The percent α-anomer was calculated using the area under the H2 peak of the two
anomers. The H2 chemical shift for each anomer was used to determine the pKa. The Ka was
calculated using the equation:
where ω is the H2 chemical shift.
2D 1H-1H NOESY at varying mixing times
A NOESY of 5 mM GlcN6P with a 0.5, 1, 1.5, 2, and 5 second mixing time of 5 mM
GlcN6P at pH 7.5 was used to determine the isomerization rate. Positive off-diagonal peaks
arise from chemical exchange between the anomers. Positive NOEs were observed between
the H2 of α-anomer and the H2 of the β-anomer. Peak volumes were determined using
Sparky (20). The rates of α to β isomer (kβα) and β to α isomer (kαβ) conversion was
calculated using the equation:
were A is matrix that contains peak volumes and R is a matrix that contains the kinetic
parameters with EXSY CALC (MESTRALAB RESEARCH) (21, 22). The optimum mixing
time (τmopt) was determined using the equation (23):
WaterLOGSY spectra
Two samples of GlcN6P were prepared at pH 7.5, one in the absence of any RNA and one in
the presence of 500 µM glmS RNA. A WaterLOGSY was performed using 512 scans of
both samples (24). The sign of the H2 proton was used to determine if GlcN6P was binding
to the RNA, a positive signal arising from interactions between the RNA and the ligand.
Only the sample containing RNA displayed any positive signals.
Davis et al.Page 3
Biochemistry. Author manuscript; available in PMC 2012 August 23.
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Page 4
Binding affinities measured by T1 relaxation rates
For the pH 5.5 GlcN6P and pH 7.5 Glc6P titrations, the ligand was added in nine steps
between 0.25 and 9 mM to 75 µM glmS RNA. For the pH titrations of GlcN6P above 5.5,
GlcN6P was added in eight steps between 0.25 and 5 mM to 35 µM glmS RNA in 550 µl.
The addition of ligand diluted the glmS RNA concentration by less than 2%. The actual
concentration of ligand was determined by using sodium acetate as an internal standard. T1
values of GlcN6P and Glc6P in the absence of RNA were measured at each pH tested and at
varying concentrations. There was little to no pH or concentration dependence on free T1
values. 512 scans were used to accurately determine T1 at low ligand concentration and only
8 scans were used at the highest concentrations. The Kd values were calculated using the
where [R]o is the concentration of glmS RNA, [L]o is the concentration of ligand, and
T1bound is the T1 of the bound ligand, τbound is the lifetime of the bound state, and ΔT1 is [1/
T1obs − 1/T1free] (38). The pH binding data were fit to the equation:
where Kapp is the observed Kd, LHKd is the binding affinity of the protonated ligand, and fKa
and bKa are the Ka of the bound and free ligand (25). This equation assumes only one
protonation state and is derived from Scheme 1. From Scheme 1 one can also derive:
Thus, if the ligand binds the deprotonated form of the ligand tighter than the protonated
form, then the pKa of the bound ligand will be lower than the pKa of the free ligand.
Reevaluation of kmax as a function of pH
The previously published glmS ribozyme kinetic data were fit to the equation:
were fHA+ is the fraction of the general acid that is protonated and fB− is the fraction of
deprotonated base and k1 is the intrinsic rate of the reaction, which is dependent on the
Brϕnsted equation (16). The data fit equally well to the more simple equation:
We determined the GlcN6P α/β isomer ratio in solution as a function of pH. If the glmS
riboswitch binds only one isomer and the isomeric ratio is pH-dependent, then the
concentration of the relevant ligand will be different at each pH. We collected 1D NMR data
of GlcN6P between pH 5.5 and 10.5 and determined the ratio of the isomers by integrating
the area under the peak for the H-2 proton (Figure 2a). The ratio of anomers, α:β, changes
from 37:63 at pH 10.5 to 64:36 at pH 5.5 (Figure 2b). The ratio is 1:1 near pH 8.0. The
Davis et al. Page 4
Biochemistry. Author manuscript; available in PMC 2012 August 23.
NIH-PA Author Manuscript
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NIH-PA Author Manuscript
Page 5
largest change in isomeric ratio occurs near the pKa of the amine (~8) while there are
smaller changes in isomeric ratio near the pKa of the phosphate (6.1). Thus, the solution pH
has complex effects on the relative concentrations of the GlcN6P isomers and their relative
protonation states.
It is possible that if the amine pKa of the two isomers in solution were different, the
observed shift in pKa could be due to isomeric preference of the glmS riboswitch. In fact,
the NMR data demonstrated that the amino pKas of the two GlcN6P isomers were not equal.
These equilibrium constants were determined by fitting the change in chemical shift of both
the 15N labeled amine nitrogen and the H-2 proton of the α- and β-isomer versus pH (data
not shown and Figure 2c). We found that the amino group pKa of the α-anomer is 8.3 and
the β-anomer is 7.8, a difference of almost half a pH unit. The pKa values of the two
anomers are just above and below the bulk solution measurement for GlcN6P amine pKa
(8.2) (7). These measurements show that the pKa of the β-anomer is closest to the observed
pKa of the cleavage reaction (7.6) (9).
GlcN6P is synthesized as the α-anomer in vivo (26). Unless the isomerization is rapid, it is
possible that GlcN6P is enzymatically converted to GlcN1P before it undergoes significant
anomerization to the β-form (1). The rates of exchange for glucose are known and are
relatively slow (t1/2 = 7 min) (27). Amino substitution (glucosamine) increases the rate by
5–10 fold and a phosphate substitution (glucose-6-phosphate or Glc6P) increases the rate by
200-fold (27, 28), but the rate of the doubly substituted GlcN6P has not been measured. We
used NOE chemical exchange to determine the rate of exchange at pH 7.5. The rate of
conversion from the α- to the β-anomer kαβ and conversion back to the α-anomer k were
first estimated at various mixing times from 0.5 to 5 seconds. These rates were used to
determine the optimum mixing time (approximately 1 second). The rates of conversion
using the optimum mixing time are kαβ = 0.06 s−1 and k = 0.08 s−1 giving an equilibration
half-life of 5 sec. These fast exchange rates indicate that both anomers of GlcN6P are likely
to be present in vivo and both would be available for binding to the glmS riboswitch. The
question then becomes; does the riboswitch differentiate between them?
We performed a WaterLOGSY NMR experiment to test if GlcN6P binding to the Bacillus
anthracis glmS riboswitch is isomer specific (Figure 3) (24). In this experiment, bulk
magnetization is transferred to the ligand though the ligand-RNA complex. If the ligand
binds to the RNA and is in fast exchange, then magnetization transfer from RNA-bound
water to RNA-bound ligand results in a positive signal. In contrast, if the ligand does not
bind the RNA, then magnetization transfer from the bulk solvent will result in a negative
ligand signal. When 500 µM glmS RNA was added to 3 mM GlcN6P at pH 7.5, 1H signals
from the α-isomer in the WaterLOGSY experiment were positive (same sign as the RNA)
while signals from the β-isomer were negative (Figure 3). Therefore, at this concentration
there was no detectable binding from the β-isomer, which suggests that the glmS riboswitch
selectively binds the α-isomer.
To further determine if GlcN6P binding is isomer specific, dissociation constants (Kd) were
determined for the α- and the β-isomer interaction with the substrate form of the glmS
riboswitch inactivated with a 2'-OMe substitution at the cleavage site. Several NMR
methods could be used to determine ligand-binding affinities; however, the large size of the
RNA and fast exchange of the ligand precluded direct observation of the RNA bound ligand.
Therefore, efforts were focused on ligand-observed NMR methods. Of the parameters
tested, the change in the 1/T1 relaxation rate of the ligand (1 to 2 seconds) in the presence of
the RNA showed the largest change. We measured the change in relaxation of the α-isomer
H1 proton and the β-isomer H2 proton as a function of GlcN6P concentration at 45 µM
glmS riboswitch (Figure 4a). The H2 proton signal was used for the β-isomer because the
Davis et al.Page 5
Biochemistry. Author manuscript; available in PMC 2012 August 23.
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text:
Advances in Microbial Physiology
Series Editor:
- Robert K. Poole, University of Sheffield, UK
First published in 1967, it is now in its 58th volume. The Editors have always striven to interpret microbial physiology in the broadest context and have never restricted the contents to "traditional" views of whole cell physiology. Now edited by Professor Robert Poole, University of Sheffield, Advances in Microbial Physiology continues to be an influential and very well reviewed series.
Microbiologists, biochemists, biotechnologists, those interested in physiology, microbial biochemistry and its applications
Book information
- Published: July 2011
- Imprint: ACADEMIC PRESS
"This series has consistently presented a well balanced account of progress in microbial physiology...invaluable for teaching purposes."--American Scientist
Table of Contents
- Novel Bacterial Merr-Like Regulators:Their Role in The Response to Carbonyl and Nitrosative Stress
- Quorum-Sensing: Regulating the Regulators Marijke Frederix and J. Allan Downie
- The Single-Domain Globin of Vitreoscilla: Augmentation of Aerobic Metabolism for Biotechnological Applications
- Singlet Oxygen Stress in Microorganisms Glaeser, J., Nuss A. M., Berghoff, B. A., and Klug, G.
- Metal Sensing in Salmonella: Implications for Pathogenesis
Alastair G. McEwan, Karrera Y. Djoko, Nathan H. Chen, Rafael L. M. Couñago, Stephen P. Kidd, Adam J. Potter and Michael P. Jennings
Alexander D. Frey, Mark Shepherd, Soile Jokipii-Lukkari, Hely Häggman and Pauli T. Kallio
Deenah Osman and Jennifer S. Cavet


text:
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The Federal Open Market Committee (FOMC) spent much of its time at the September 16-17 meeting debating the timing of when to begin normalizing short-term rates. Some participants argued that there were still sufficient downside risks in the economy and that inflationary pressures remained minimal – well below the Fed's stated goal of two percent. Therefore, it did not make sense for the FOMC to wait for better signs of strength in the U.S. economy before starting the process of raising rates. This process was often referred to in the minutes as "policy firming." In contrast, others said that "a prompt decision to firm policy could provide a signal of confidence in the strength of the U.S. economy that might spur rather than restrain economic activity." Those same individuals also worried about the long-term pricing consequences of continuing to keep short-term rates near zero for so long. (continue reading…)
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Hotel information
Hotel facilities
- Elevator
- Public Internet terminal, Hourly rate 1 EUR
- WLAN with Internet access in lobby, Hourly rate: 1 EUR
- Total number of rooms: 34
- Number of single rooms: 4
Room facilities
- WLAN with Internet access in rooms
- Satellite TV
- Pay-TV
- TV remote control
- Telephone by the bed
In-house services
Show all
Style of hotel and ambience
Get a good night's sleep in the simply furnished, yet lovingly decorated rooms of the hotel Valmarina. The friendly staff is known for serving its guests a tasty breakfast in the morning and assisting them with any questions or requests. Wi-Fi access and parking facilities round off the hotel's services.
Special features of the hotel
The only one Hotel in Calenzano with private garage inside the structure, offers service bus tickets from and for Florence city, Internet point etc, in an environment family parish priest and calm, open 24H.
Hotel details
Last partial renovation: 2008, Number of floors: 2, Total number of rooms: 34, Number of single rooms: 4, Number of double rooms: 30, of which have 2 separate beds: 18, of which have a double bed: 12, of which are suitable as three-bed rooms: 7, No-smoking room: 0,
Service times
Reception open:
00:00 - 24:00
Reception manned at weekends:
00:00 - 24:00
Earliest check-in:
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Jordyn Glenz and Sydney Weniger combined for four hits and seven RBIs Thursday as the Bertha-Hewitt/Verndale Raiders stayed alive in the Section 6A softball tournament by knocking off Brandon-Evansville 12-7. The Raiders were clinging to a 6-4 lead in the top of the fifth when they erupted for six runs. The Raiders sent 10 batters to the plate in their breakout inning.
Jordan Kern shot an 86 and three of his teammates carded career-best rounds Monday as the Wadena-Deer Creek Wolverines placed fourth in the last Mid-Minn Conference golf meet of the season at Cragun's Lodge. Kern's round left him one stroke away from all-conference honors. Shooting career-best rounds on the Cragun's Legacy course were WDC's Thomas Quincer (88), Dawson Lupkes (99) and Landon Blessing (148). Jack Goeden fired a 108 for the Wolverines. Staples-Motley's Andrew Israelson took meet and season medalist honors. Israelson carded a 69.
Morgan Hess went the distance as top-seeded Minnewaska Area ousted eighth-seeded Wadena-Deer Creek from Section 6AA tournament play Tuesday with a 3-1 fastpitch win. Hess surrendered five hits while striking out two in her seven innings of work. Morgan Zeise pitched a complete game for the Wolverines. Zeise did not allow a walk and fanned two. Casey Volkmann and Maddy Hinojos led the Wolverines with two hits apiece. Ashley Peters drove in Volkmann with WDC's lone run in the top of the first. The Wolverines held onto that lead until the fourth when the Lakers managed to tie the game.
Jess Rondestvedt shot a 96 and Gabi Ross carded a 98 Tuesday as Wadena-Deer Creek placed sixth in the 11-team Detroit Lakes Invitational. McKayla Woods and Jess Langer brought in rounds of 104 and 108 for the Wolverines who posted a team score of 406. Pequot Lakes' Maggie Heggerston fired a 73 for medalist honors. Alex Stone and Amanda Nies posted scores of 84 and 89 for Pequot. The Patriots won the invitational with a team round of 336.
The Wadena-Deer Creek Wolverines out-hit Parkers Prairie 11-9 Thursday evening but lost a 12-11 slugfest to the Panthers on the WDC diamond. Jake Dykhoff paced the Wolverines at the plate with two hits in four trips, two runs and two RBIs. Zac Schertler went 2-for-3 at the plate with a double and one RBI. Errors were a problem for both clubs with the Panthers committing six and the Wolverines four. Matt Ferley chalked up the mound win while Noah Ross was hung with the loss. The Wolverines closed out their record season schedule with five straight losses and a 4-13 record.
Marc Reynolds saw six of his Wadena-Deer Creek track and field athletes post victories Thursday in the Sub-Section 6A meet at Pillager. Autumn Jahnke placed first in the pole vault with a 10-0 leap.
The Bertha Blazers gained a Hi-10 League split with Bluffton Saturday as they shook off a 2-1 loss in the opener to for a 10-0 romp in the nightcap. Strong...
Courtney Templin, Rumor Stokes and Samuel Moore notched individual victories Friday as the Bertha-Hewitt/Verndale track teams placed third and fourth in the Park Region Conference meet at Sebeka. Templin ran a winning time of 1:02.60 to edge United North Central's Andrea Korvela who turned in a 1:02.88. Stokes was a double winner for the Raiders with a 15-4.75 effort in the long jump and a 33-9.5 in the triple. Moore was king pin in the weights with a winning 49-6 in the shot put and a 155-3.5 in the discus. BHV head coach Ken Miller saw his 4x200 relay team of Stokes, Templin, Erica VanDe
Preston Riewer went 2-for-4 and drove in three runs Friday as Bertha-Hewitt/Verndale defeated Nevis 8-2. Mitchell Blaha, Mack Jones and Matt Lipinski combined for five runs, five hits and four RBIs in the victory. Dillon Card fanned eight in five innings of work to gain the mound win.


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H.R.2893 - Health Insurance Reform Act of 1996104th Congress (1995-1996)
|Rep. Roukema, Marge [R-NJ-5] (Introduced 01/25/1996)
|House - Commerce; Ways and Means; Economic and Educational
|Latest Action:
|02/09/1996 Referred to the Subcommittee on Employer-Employee Relations.
This bill has the status Introduced
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Cosponsors: H.R.2893 — 104th Congress (1995-1996)All Bill Information (Except Text)
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Come out and enjoy a day of riding with friends and family. See natures at its best; a hawk flying over, fish jumping in the lake, a deer running down a path. Feel the wind on your face as you canter your Arabian horse in South Florida's back country
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Right from the start, Deadspin lets you know that this preview is not for Eagles fans.
It is, however, part of their ongoing series of 2013 NFL team previews, in which they take a look at all the various reasons each NFL team sucks. Today was the Eagles' turn. Here's a look at some highlights, but make sure to check out the whole thing here.
On the fans:
Getting rid of Reid took ages, so you would think that Eagles fans would be excited for Kelly and give him at least a year to get his house in order. But Eagles fans are the most ungrateful people alive. Your typical Eagles fan is a bloated, sweating neckbeard who spends the entire game frowning with his arms crossed, regardless of the score.


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We've found 12 marvellously festive lesson ideas that combine seasonal frivolity with an educational twist
The Muppets, festive alphabets and snowmen marches – there are plenty of ways to bring the sound of music into your Christmas classroom
As the temperature drops and the nights draw in, welcome winter into your classroom with lots of teaching ideas and resources
Looking to add some final touches to your pupils' nativity outfits? Here's how to make them look great with breaking the bank
Educating students about the UK's diverse mix of cultures promotes inclusion and tolerance. Take your pick of our resources
As Anti-Bullying Week gets under way, here's our guide for teachers on how to encourage inclusion and respect in your classroom
It can be difficult to know where to start for teachers offering advice to students about their future career prospects. Here are our best resources on the subject
For practical tips on how to be a great teacher and have a life (without feeling guilty), join our live chat on Wednesday November 12 from 5.30-7.30pm
A new report from the Sutton Trust has reviewed the evidence around successful teaching practices. Here are the key points you need to know
Teacher's blog What makes great teaching? – expert viewsSarah Marsh and Kate HodgeA new report has reviewed all the research into what makes teaching effective. Our experts share their thoughts about the findings – have your say in the comments thread or tweet us @GuardianTeach
Research shows 90% of primary and secondary school staff have had no training to support LGBT students. Teacher Allie George explores how to make your classroom safe and inclusive
Using film in the classroom isn't a perk reserved for English and drama. Teacher Elizabeth Evans explores how science, geography and maths students can benefit
Need some out-of-this-world lesson ideas to help you teach about space and the planets? Here are the best resources from the Guardian Teacher Network
Bake your own bread or track the journey of food around the world – we offer a basket of fresh ideas for exploring the harvest
School librarians are depressingly underused, argues Sally Dring. Many teachers would be amazed at how much support they can give them and their students
There's no substitute for the sense of wonder on first seeing the Great Wall, says teacher Matthew Bell, plans to visit every country in the world to bring geography to life for his students
Whether it's a new school or moving into secondary education, transition is unavoidable. Here are some resources for teachers to help students cope with change
- Secondary schools
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- The role of film in schools
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Photo moment of the Day: Rocky Mountain Sustainable Living Fair.
This photo was from last year. We're not attending this year, doing videos, media sponsoring or doing any coverage as in past years…we're getting ready for Yoga Journal conference, and covering the Natural Products Expo East, as well. Still, I wanted to give a shout-out to the Sustainable Living Fair, where my longtime buddy ol'pal Joshua Onysko is a featured speaker—it's a great time, and I highly recommend you go, if you happen to be around Ft. Collins, Colorado.
Click photo for link.
Incorrect source, offensive, or found a typo? Email us (please put title in subject bar of email so we'll be able to fix). Or do you want to write for Elephant?
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Thanks a lot you guys! You really busted it out


text: I stayed 4 years ago for our FACET weekend and loved it, so we booked again for graduation. Had the whole family, great room, good cookies and breakfast - but the best surprise of all was on the day of graduation. We thought we were going to have to fight to park, or walk across campus in pouring rain, but when we got to the lobby they announced that there was a shuttle to graduation. (The Ga Tech conference center didn't even provide that!). Brandon handled the chaos with patience when too many people needed a ride back in the cold rain. It really saved our day, and I am so thankful. The ladies at the desk were very nice and helpful (I forgot their names, but we checked in May 3rd). Just a great stay - my only complaint is that I did not know valet was mandatory, and I didn't think it was necessary. Thank you all very much!!


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The AIS/PRISim (Professional Range Instruction Simulator) system offers some of the most realistic training available in the use-of-force simulator market today. The system uses MPEGII quality video with up-to-date scenarios and an ever-expanding library to choose from. PRISim incorporates a Shootback cannon that fires a 68-caliber nylon ball at the trainee when the scenario shoots, reminding the trainee that their cover is anything but perfect. The entire scenario can be played back showing the trainee, on screen, in a small capture window (picture in picture), allowing evaluation of the trainee alongside the scenario. AIS offers OC, baton and flashlight options to cover the entire use-of-force continuum.
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text: Daughter of the Vampire-Chapter 1Chapter 1Sequestered Kahsiah stared at herself in her mirror. All she could see was her black outfit, but she knew she had her father's jet-black hair and slender build, and her mother's chocolate brown eyes and elfin facial features. She also knew of her cadaverous complexion, and white hands with fingernails like claws. "Damn this isolation! Damn this prison! Damn Elizabeth!" Kahsiah shouted as she struck her mirror with her fist, shattering it and leaving her hand with a bloody cut. "Damn Elizabeth for chasing my parents away and locking me up here!" "Kahsiah! Keep your voice down! You may sleep through the day, but the rest of us have to sleep right now to survive the day!" "Get out." "Kahsiah, I do believe Vicky has taught you countless times not to bite the hand that feeds you." "No promises, I'm thirsty." Kahsiah meant i


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The shooting in Oregon has --once again-- raised the call for more stringent gun laws. But where does Hawaii stand in our safety measures for gun ownership?
At least 73 people are dead and many more are missing after a massive landslide covered much of a town in Guatemala, according to the nation's Public Ministry.
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Early Automation Approach
An early automation approach involves the automation team in the early phase of the testing lifecycle to support agile or iterative projects. Automation scripting can commence in parallel to system development.
Senthilkumar Gopalakrishnan
July 14, 2011
Branching to Distraction
Branching can be an effective solution for managing change, enabling parallel development and improved productivity. But, working on a branch is a distraction and can decrease agility, productivity, and code robustness. Learn when the value of working on a branch outweighs the cost.
Steve Berczuk
July 10, 2011
Four Techniques to Wrap Your Head Around Complicated Code
Software people love challenges and want to exercise their brains by tackling difficult problems. Our nature is to understand complicated problems, become familiar with various business domains, and generate a solution that helps the world become a better place. Nirav Assar explains four techniques to wrap your head around complicated code.
Nirav Assar
July 6, 2011
Simulation Games: A Way to Improve Communication in the Team
One of the hardest daily tasks developers, QA, ScrumMasters, and product owners encounter is effective communication with others. Sound implausible? According to many articles, research, and personal observations, the main cause of project failure is not technology or hardware, but inefficient communication stemming from lack of effective communication between team members, incomplete business analysis, imprecise requirements, and vaguely formulated business objectives.
Monika Konieczny
June 10, 2011
Four Agile Tips to Eliminate Rework in Application Development
Your applications need to meet business needs, overcome complex processes, and provide instant results to customers. And, ideally, they'll require minimal rework on your part. The first step to success is requirements definition. Here, Filip Szymanski offers some tips from agile methods that will improve your requirements—even if you haven't otherwise adopted agile.
Filip Szymanski
June 6, 2011
Adapting to Change in Your Agile Strategies
Len Whitmore writes on using agile practices for the development of software. In the ten years since the Agile Manifesto, the agile development domain evolved, as evidenced by such things as the six levels of planning: strategy, release, iteration, daily, and continuous, with strategy appearing to be the least evolved of the planning levels.
Len Whitmore
June 3, 2011
Experimenting: The Way Forward for Agile Development Teams
If you asked anyone in my team what agile practice is most responsible for our success over the past eight years, I bet they'd answer "retrospectives". At the start of every two-week sprint, we spend time talking about the previous sprint, identifying areas that need improvement, and thiinking of ways to overcome obstacles. But I wonder if it's not so much the retrospectives themselves, as the small experiments (to borrow Linda Rising's term) we try to address our problem areas.
Lisa Crispin
June 1, 2011
Mission Possible: ScrumMaster and Technical Contributor
Teams trying out Scrum might not be able to justify a full-time ScrumMaster to the organization, so the role is filled by a contributor on the team. This can be a challenge and, if done incorrectly, a problem. Learn some potential issues to be aware of and how to make the hybrid role work.
Steve Berczuk
April 25, 2011
Personality Factors That Influence Core Build and Release Management Practices
Leslie Sachs discusses the key people skills essential to appreciating how and which personality factors most impact one's ability to successfully implement core build and release management practices.
Leslie Sachs
April 18, 2011
The Competition of Agile
It is sensible to want to avoid the head-butting sort of competition—that is, arguing for the sake of arguing. But, differing opinions and styles can be a good thing. Competitive forces have driven markets, innovation, and civilization for millennia. Here, Jurgen Appelo takes a look at some of the various approaches to agile development and what they bring to the table.
Jurgen Appelo
March 16, 2011


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In days of yore, before the first JAP communed with a pair of Blahniks in the sanctum sanctorum of Bergdorf's, Jews appreciated the metaphysical subtleties and theological niceties of footwear. In Jews and Shoes (Berg, $35), an odd collection of essays by Jewologists, folklorists, and an interdisciplinary mélange of cultural historians, editor Edna Nahshon cobbles together a surprisingly rich account of the Tribe's journey via their footwear. Who knew?
The Jew-and-shoe story kicks off with Moses's first divine order: "Draw not nigh hither; put off thy shoes from off thy feet, for the place whereon thou standest is holy ground." Ora Horn Prouser's fascinating read of "The Biblical Shoe" sorts out this overdetermined gesture: Humility, lack of choice, unpreparedness, a covenantal legal relationship, and
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Welcome to the MaCS Program, a specialized program at William Lyon Mackenzie C.I.
President Entrance Scholarshiphttp://wlmac.ca/macsprogram/files/2015/09/Brandon-Chang-Grad-Photo-4.jpg
Congratulations to Brandon Chang (MaCS Alumni 2015) for receiving the President's Scholarship at the University of Western. The scholarship is awarded to students who exhibit a passion for learning and a commitment to school and community life. They are awarded based on outstanding academic performance. We wish Brandon and all of our graduates the best of luck as they begin their first year of study at their post-secondary university.
Grade 12 MaCS Triphttp://wlmac.ca/macsprogram/files/2015/05/macs.jpg
On Wednesday May 13th, Grade 12 MaCS students had the opportunity to see an evening performance of the musical Once at the Ed Mirvish Theatre and have dinner at Quinn's Steakhouse!
Waterloo Scholarshipshttp://wlmac.ca/macsprogram/files/2015/05/IMG_20150514_110142.jpg
A representative from the Faculty of Mathematics at The University of Waterloo personally dropped by W.L. Mackenzie C.I. to hand deliver scholarships totaling $37, 000 in addition to the scholarships they already received with their offer of admission. Congratulations to the following MaCS students: Matthew Chung, Benson Guo, Peter Huang, Vivian Ngo, Ryan Odd, Reyno Tilikaynen, and Erik Trinh.
National Biology Competitionhttp://wlmac.ca/macsprogram/files/2015/05/Untitled-11.jpg
Congratulations to all MaCS students who participated in the University of Toronto National Biology Contest last month! The results are in and Mackenzie placed second in the TDSB and 9th in Canada!
Western Math, Science, and Engineering Olympics 2015http://wlmac.ca/macsprogram/files/2015/06/group-5.jpg
Congratulations to all the MaCS students who attended the University of Western Ontario Math, Science, and Engineering Olympics this past Thursday! MaCS students medaled in 7 events!
Grade 8 MaCS Orientationhttp://wlmac.ca/macsprogram/files/2015/06/MaCS-orientation-21.jpg
On Friday May 29th all incoming grade 9 MaCS students were invited to attend our annual grade 8 MaCS Orientation. Students had the opportunity to tour the school, meet their peers, and learn more about the MaCS program and Mackenzie. The afternoon session ended with a BBQ! We look forward to welcoming our incoming students in September!
Chem 13http://wlmac.ca/macsprogram/files/2015/06/Chem-13.jpg
Congratulations to the following MaCS students who placed in the top 20% Nationally on the CHEM 13 University of Waterloo Chemistry Contest: Gil Posluns, Sepi Mortazai, Sejin Kim, and Anqi Wu.
MaCS Graduationhttp://wlmac.ca/macsprogram/files/2015/06/MaCS-Grad-2.jpg
This past Friday our Grade 12 MaCS students got together as a group before final exams at our MaCS graduation! We wish all of our MaCS graduates the best of luck in all of their future endeavours!
Math Contestshttp://wlmac.ca/macsprogram/files/2015/06/PicMonkey-Collage.png
Congratulations to all our MaCS students who wrote math contests this past month. Click this slide to see their results!
Go Reyno!http://wlmac.ca/macsprogram/files/2015/05/Reyno-21.jpg
We want to wish Reyno Tilikaynen (Grade 12 MaCS) good luck this week at the University of Waterloo where he is competing for a spot on the International Canadian Computing Olympiad Team.
ECOO Provincialshttp://wlmac.ca/macsprogram/files/2015/05/ecoo.jpg
Congratulations to Joseph Chu (Grade 12 MaCS), Matthew Chung (Grade 12 MaCS), Benson Guo (Grade 12 MaCS), and Reyno Tilikaynen (Grade 12 MaCS) for winning the bronze medal at the ECOO Provincial Championships this past weekend!
Ontario Secondary Schools Spanish Contesthttp://wlmac.ca/macsprogram/files/2015/05/Spanish-contest.jpg
On May 7, 2015, 7 Mackenzie students took part at the Ontario Secondary Schools Spanish Contest held at York University Glendon Campus. Mackenzie got 5 of the top 10 awards! Congrats to the following MaCS students: Jacqueline Hong, Uma Kalkar, Nahal Ehnami, Jordan Cotton, and Roi Levy for their outstanding accomplishment!
UBC Michael Smith Challengehttp://wlmac.ca/macsprogram/files/2015/05/Michael-Smith-Challenge.png
The Results for the UBC Michael Smith Challenge Grade 10 Science Contest have been announced. Congratulations to the following grade 10 MaCS students: In the top 1% nationally - Abdul Arif, In the top 10% nationally - Madeleine Smee, Ryan Jiang, Nikola Damjanovic, and Julie Nguyen
Grade 11 MaCS Triphttp://wlmac.ca/macsprogram/files/2015/05/MaCS-11.jpg
This past week the grade 11 MaCS students had a great three day trip to Cleveland! Students had the opportunity to gain a historical and cultural perspective of the city (Rock and Roll Hall of Fame, Soldiers and Sailors Monuments, Museum of Natural History and Art, etc.).
Congratulations to Travis Kuhne (Grade 12 MaCS student) for placing in the top ten at the DECA Championships in Orlando Florida this past week!
ExploraVision Science and Technology Competitionhttp://wlmac.ca/macsprogram/files/2015/04/Exploravision.png
Congratulations to the following groups of MaCS students for receiving an Honourable Mention at this year's Toshiba ExploraVision Science and Technology Competition: Project - Natural Cancer Senescence Treatment (NCST): Gilead Posluns, Helen Ma, Serena Leo, Stanley Wu. Project - Regenerative Epigenetics and Dedifferentiation (REaD): Max Midwinter, Joanne Nguyen, Eric Tseng, An Qi Wu
Waterloo Canadian Computing Olympiadhttp://wlmac.ca/macsprogram/files/2015/04/Reyno1.png
Congratulations to Reyno Tilikaynen (Grade 12 MaCS student) for advancing to the next level in the Canadian Computing Competition. Reyno has been invited to the University of Waterloo next month and will be competing for a spot on the Canadian Team!
Ontario Model Parliamenthttp://wlmac.ca/macsprogram/files/2015/04/OMP.png
Some of our MaCS students had the opportunity to take part in a three day trip to Queens' Park. Students had the opportunity to participate in a mock legislative procedure for a three day period.
ECOO Computer Programming Competitionhttp://wlmac.ca/macsprogram/files/2015/04/Untitled-11.png
Congratulations to our MaCS students who participated in the ECOO Computer Programming Competition during the weekend of March 28th to March 29th. All of our teams have advanced to the Regional Championship!
Mackenzie Science and Engineering Olympicshttp://wlmac.ca/macsprogram/files/2015/03/zma.png
On Tuesday March 10th Mackenzie hosted its 9th annual MSEO (Middle School Math, Science and Engineering Olympiad). Eleven middle schools participated and over 115 Mackenzie students volunteered their time to put on this great event! Congratulations to all those who participated!
Mattawa Triphttp://wlmac.ca/macsprogram/files/2015/03/zmac.jpg
Our grade 10 MaCS students spent three days at the Canadian Ecology Centre participating in a high-tech winter ecology program. It was an amazing trip with a lot of fun and challenging activities!
Gene Researcher for a Weekhttp://wlmac.ca/macsprogram/files/2015/03/IMG_20150226_1218251.png
Congratulations to Sabrina Cancelliere (Grade 11 MaCS) and Katya Potapov (Grade 11 MaCS) for being selected to participate in the Gene Researcher for a Week program - a week-long mentorship in one of Canada's top genetic labs. They will be learning about genetic research by conducting hands-on science experiments at the Women's College Research Institute and the Hospital for Sick Children. They are very excited to be participating in this amazing program over the March Break!
Alumni Successhttp://wlmac.ca/macsprogram/files/2015/02/n110113-sri-winners-banner.png
Congratulations to Kramay Patel (MaCS Alumni 2012) for winning the $5,000 Sunnybrook Research Prize. For more information please go to the Sunnybrook Hospital website: (http://sunnybrook.ca/research/media/item.asp?c=2&i=1234&f=would-be-scientists-share-enthusiasm-science)
SHAD Valley Summer Enrichment Programhttp://wlmac.ca/macsprogram/files/2015/02/IMG_20150218_120900-1.jpg
Congratulations to Isis So (Grade 11 MaCS student) and Aditi Sriram (Grade 10 MaCS student) for being selected by SHAD Valley to participate in their summer university enrichment program!
Provincial DECA Championshipshttp://wlmac.ca/macsprogram/files/2015/02/dec2.jpg
This past weekend the Provincial DECA Competition was held in Toronto. Congratulations to Travis Kuhne (Grade 12 MaCS student), Max Verzunov (Grade 11 MaCS student), Aditi Sriram (Grade 11 MaCS student), Sabrina Cancelliere (Grade 11 MaCS student), James Im (Grade 11 MaCS student), and Lucy Chen (Grade 10 MaCS student) for moving on to the International Championships which will be held in Orlando Florida this April!
Gold Medal Win at the PanAmerican Gameshttp://wlmac.ca/macsprogram/files/2015/02/image001.jpg
Nikola Damjanovic (Grade 10 MaCS student) and his team participated at the Pan American Games this past weekend. As part of the Canadian Junior fencing team, Nikola earned a gold medal on Sunday. Congratulations to Nikola and his team!
MaCS Entrance Examhttp://wlmac.ca/macsprogram/files/2014/12/image-1.png
Over 500 grade 8 students from all over the GTA registered for the MaCS program and arrived on Saturday December 6th to write the entrance test for the program and submit their application package. All applicants will be notified the week of February 1st 2015. Thank you to all those who applied!
Hour of Codehttp://wlmac.ca/macsprogram/files/2014/12/hour-of-code-001.png
From December 8th to December 9th, 200 students participated in the Hour of Code initiative, which was open to programming beginners and masters alike. The event was run by 25 senior MaCS students from the Math, Computer Programming, and STEM clubs.
Motivational Assemblyhttp://wlmac.ca/macsprogram/files/2014/11/Blake-Fly-20.png
Blake Fly, a motivational and TEDx speaker and author came to talk to our grade 11 and 12 students about the transition from high school to university. Blake really connected with the students and gave them practical success strategies for university life. Graduating MaCS students walked out of the assembly feeling empowered to finish strong.
Canadian Senior/Intermediate Mathematics Contesthttp://wlmac.ca/macsprogram/files/2014/11/IMG_07301.png
Over 150 MaCS students participated in the Canadian Senior (CSMC) and the Intermediate Mathematics (CIMC) Contests. Students were given two hours to complete nine advanced math problems and demonstrate their understanding of different concepts.
Waterloo Math Workshophttp://wlmac.ca/macsprogram/files/2014/11/IMG_07221.png
MaCS students in grades 9 through 12 attended a Waterloo math workshop where a math professor from the University of Waterloo talked to them about improving their problem solving skills and preparing them for the Canadian Senior/Intermediate Mathematics Contest. Students left with new strategies for contest taking and for writing proofs in math.
Canadian Open Mathematics Competitionhttp://wlmac.ca/macsprogram/files/2014/11/COMC1.jpg
Many MaCS students participated in the Canadian Open Mathematics Challenge. They had been preparing rigorously and we look forward to receiving the results in the next few months!
MaCS Information Nighthttp://wlmac.ca/macsprogram/files/2014/11/macsinfonight1.png
It was standing room only in our cafeteria and library as more than one thousand parents/guardians and potential MaCS students gathered to learn more about our MaCS program and how to apply. Current MaCS students and MaCS Alumni spoke about their experiences in the program and answered questions from the audience.
McMaster Engineering & Science Olympicshttp://wlmac.ca/macsprogram/files/2014/11/McMaster-SO.jpg
Congratulations to all the students who participated in the McMaster Engineering & Science Olympics at McMaster University. Students competed in many events, worked hard with their teams, and had an amazing time! Special mention to the Scavenger Hunt Team and the Monsoon Challenge Team for placing first in their event.
On Halloween, MaCS Students dressed up, participated in spooky activities and contests, and enjoyed a variety of food and refreshments.
DNA Fingerprinting Labhttp://wlmac.ca/macsprogram/files/2014/11/10754783_727083917385284_1622079352_n.jpg
Some of our Grade 12 MaCS students had an expedition to the Ontario Science Centre. At the Science Centre, students performed PCR tests using their cheek cells to determine the frequency of one of their genotypes. Students also used k'nex to build a model of the DNA helix.
Grade 12 Catalyst Labhttp://wlmac.ca/macsprogram/files/slideshow-gallery/wp001620.jpg
Our Grade 12 MaCS Biology students completed a lab where they investigated how different environmental conditions affect the enzymatic activity of catalase.
Muskoka Woodshttp://wlmac.ca/macsprogram/files/slideshow-gallery/muskoka.jpg
The Grade 9 MaCS students recently embarked on the annual trip to Muskoka Woods. At the resort, students enjoyed the opportunity to develop their leadership skills through a variety of activities! See more at the gallery.
Toronto Urban Film Festivalhttp://wlmac.ca/macsprogram/files/slideshow-gallery/00jpgsrz1.jpg
Congratulations to Jonah Haber (Grade 12 MaCS Student) whose film "James" is currently being featured at the Toronto Urban Film Festival (TUFF)!
With over 350 students, a good portion of the student population at Mackenzie is part of this program. This program is one of a kind, giving students many opportunities both academically and non-academically. Please feel free to take the time to search the site and learn about the many possibilities that are waiting for you. For a start, look at our Gallery Page for pictures and descriptions of a variety of activities/events our students take part in.
If you are interested in being a part of this elite program, please go to the Application Page for more information.
For present students, please go forward to the Current Events Page.
Thank you and I hope you enjoy your experience.
S. Leder
MaCS Coordinator
W.L. Mackenzie C.I.


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Photo by Romulo Yanes
The vitamin C tablet in this recipe keeps the apple juice from turning brown.
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Nice recipe to impress guests. You can combine instant apple cider powder with the syrup, gives it a nice touch.flag if inappropriate
Very nice recipe, I added sour apple liquor for color and more flavourflag if inappropriate


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Capital Markets
Company Overview of Amcap Fund
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Senior Software Developer
Mexico - Tests: 5
I'm a full-stack software developer. I use to follow and implement code standards and also to audit and modify code for a better performance, knowledge and understanding. The best part is that i love what i do as i live for it. It's like i'm beeing payed for having lots of fun. Wait, that's true in fact!!! I love technology and development innovation and i when i'm sleeping i dream about code, implementations and deployments. I have experience developing professional enterprise software solutions under many technologies, mainly with Java and Ruby On Rails. I have good written and verbal english communication skills. I consider myself as proactive, responsible and can be part of any team work. It doesn't matter what's the problem to solve or what's the programming language to solve it, i can learn it and will solve it in the best possible way.