text:
Sep 14, 2010 GMTdotEPUB is a simple yet genuinely useful Chrome Extension and browser bookmarklet that lets you save the currently viewed Web page in the EPUB format. That's pretty much all it does: there are no other features or options to tweak. Hit the button (or click on the bookmarklet), and dotEPUB saves the current Web page as a nicely formatted EPUB document. You can then read it on any device using a reader that supports the EPUB format. dotEPUB provides a perfect "read it later" solution which you can use to save interesting articles and pages and peruse them later on any device at hand. dotEPUB is also available as a widget, so you can embed it into your Web site and give your...
Productivity Sauce
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Aug 13, 2010 GMTUbuntu One may not be as popular or feature-rich as Dropbox, but the file synchronization service bundled with Ubuntu has one nifty feature that makes it a handy tool -- the ability to synchronize Tomboy notes across multiple machines. To make use of this feature, you have to enable and configure the synchronization options in each instance of Tomboy. To do this, launch Tomboy, right-click on its icon in the gnome panel, and choose Preferences. Switch to the Synchronization section, select Tomboy Web from the Service drop-down list, and press the Connect button. Authorize your machine with the Ubuntu One service, switch back to the Preferences window and press Save. Do the same on other...
Aug 12, 2010 GMTSMART-capable hard disks are commonplace these days, and there are a few tools that can help you to use this technology to monitor the health of your hard disk. The smartmontools toolset, for example, lets you monitor and manage hard disks from the command line, while the GSmartControl utility can be used to keep a close eye on the hard disks. Despite its simple interface, GSmartControl is a rather capable tool that automatically reports and highlights any anomalies, performs short self-checks every four hours, and provides comprehensive info about the hard disks and their capabilities. The best part is that GSmartControl makes it supremely easy to monitor your hard disk's health. When...
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Issue 180/2015
Buy this issue as a PDF
Dyreza Malware is Back
Powerful man-in-the-middle attack is now targeting online shopping.
Kernel Developer Matthew Garrett Forks the Linux Kernel
Another high-profile coder says the kernel team needs a kinder, gentler culture.
Bugzilla Bug
Bug database has a bug of its own that could allow an intruder to create an unauthorized account.
US Launches New Strategy for Broadband Access
Report focuses federal resources on achieving universal Internet access.
RC4 Finally Gets the Fork
Leading browser makers say "no" to porous encryption algorithm
IBM Says TOR Network a Vehicle for Ransomware
Report from the X-Force group says attackers are using TOR to hide their crimes
Mozilla Announces new Firefox API
Future Firefox extensions will be compatible with Chrome.
Weird Security Flaw Affected Intel Chips for 16 Years
Better read this if you bought your computer before 2011
OpenSSH 7.0 Secure Shell Arrives
Users should upgrade to the new version as soon as possible
Qemu Flaw Lets the Guest Escape
Xen project announces a privilege escalation problem for Qemu host systems


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A classic platform style which is timeless in its appeal. Shown here with a Zac Posen dress and Devi Kroell bag. Sizing is Italian.
Black leather platform pumps, approximately 120mm high. Christian Louboutin shoes have a red peep-toe and hidden 20mm platform
Product code: 27351 - Need help? Contact us

subset: commoncrawl


text:
EDIT: Sorry to constantly spam your inbox with this one. Some of you may have noticed the animation so far has slightly more detail than was on the previous character sheet, and I've finally gotten around to updating this reference to match. Louis's tights are now off-white to differentiate them from his boots, and James has epaulettes this time around. I also took the liberty of adding a point of reference for Louis's height sans heels, since many people have been asking about it; he is shorter than Santiago, but taller than James.
A compilation of turnarounds for all three main characters from my upcoming animation this semester at LASALLE, College of the Arts, plus a height chart to show their relation to one another. The final animation will be over three minutes long, animated on TV Paint; hopefully adding shine to Santiago's armor will be easier there than using traditional mediums. James's uniform has been altered slightly to be more historically accurate based on this illustration: [link] and Louis is ... Louis.
All characters © Myself, Shamine A. King
For LASALLE, College of the Arts, Singapore
this one is good.. well done bro...
I love character sheets like these, and they are all just fantastic (especially Louis, of course, but we already knew that). . U .
I love how Louis towers over everyone despite being the daintiest.
*hides behind Louis* Santiago comes by me where he'd sooner snap a sword in half than actually use it.
Ohhh, this looks really nice! A really good way to present them, too, love this parchment-y style... It will be so fun to se more of these guys! ^^
I'm smashing my head into the keyboard wondering how you draw people so well. *smash smash smash*
Not my wrecking my face! D8 *YANKS HEAD BACK UP* Just practice! D:>
I'd like to see james in a "redcoat" character costume from assassin's creed 3
I'm always afraid of what might happen when Louis meets Santiago, like I know Louis is crafty enough to get out of a sticky situation but I get the feeling that Santiago would snap him in half like the toothpick he is!
HUZZAH EPAULETTES! I love this.
Now I can only imagine them playing Warcraft or something with Santa de la Cruz being an orc, James being a Dwarf/human and Louis being the blodo elf.. o.o
These are incredible. Will the final animation be posted online as part of the school's website or on YouTube?
I'm uploading it's progress so far to Youtube, but I've only sketched out 3 of the total 10 minutes.
Fantastic! It's definitely a huge undertaking but I have no doubts that it will look amazing once it's completed!
*takes notes* Off white pants...got it!
James is the shortest even when Louis isn't wearing his heels...does Louis ever make fun of him about it?
Hey, Rainwater, you could repeat what the theme of HAT is, again? I know you've been asked this a hundred times, but I'm still not getting a proper hold. I think I remember you once said it wasn't anti-religious and that is was supposed to be a representation of how homosexual people aren't as silly and stupid as people mark them to be, but i'm not quite getting the vibe. Care to elaborate?
Honour Amongst Thieves was initially inspired by gender inequality in animation and the media. But the concept has since developed to address issues related to religion, the government, and liberal thinking. The story now warns about letting power and greed get to your head; both government and religion underestimate the people to get what they want, and they are their own undoing, which the people just want to remind these two superpowers who gives them support and who enacted them in the first place.
Ah. Thank you for the explanation. I was sort of getting the vibe of "religion and government constrict and hold back" when Louis said something around, "I don't need God or the government to...."
That comment is directed at the hypocrisy within government and religion, Santiago both out to rob each other of gold that doesn't belong to them in the first place, yet they feel justified in their actions because they believe they are working for a higher power. Louis is not disillusioned with the idea that he is somehow above the other two simply because they don't agree with his philosophies, which is more than he can say for James and Santiago.
Okay. Yeah, see, cause I was thinking that he was in this mindset of "Well you two are petty, blind little followers of God and country and I, I the independent, fabulous Louis am above you two because I don't need God or government to tell me what to do, so screw you all." And I was thinking to myself, "That isn't the Louis I remember during production."
"W/out heels, Louis can almost look Santiago in the eyes" - I bet if he abandoned contrapose and stood straight (xD), he might have been able to do so. I hope he never does that, though xD It's essence of Louisness
curius why do you leave the arms off on the side view? Are you tought to do this?
You need to see the entire body, and arms get in the way/hide the detail.
Taking away the arm for the side allows for the observer to better see the figure from that point without the arm obstructing the view.
We took photos last weekend of British naval uniforms, right around this period, at a museum.
It's so tough to find accurate costumes for me, a lot of fashion seems to get mingled. I'm going to be hitting up the documentaries and going to museums before this comic gets underway.
Amazing! u_u
I love your character sheets so much! You're my favorite artist.
I recently discovered you and i find this comic amazing!!! Great work with the characters and an unusual story!
Can we see more scenes? Please??????
I recently discovered you and i find this comic amazing!!! Great work with the characters and an unusual story!
Can we see more scenes? Please??????
I love how you made their anatomy starkly different! Heavy, medium and small. All builds, all proportional ideas! I'm really digging how you made Louis with model-proportions.
I love how you made their anatomy starkly different! Heavy, medium and small. All builds, all proportional ideas! I'm really digging how you made Louis with model-proportions.
This is really cool! I like the background on who they are in addition to the details like "# of heads wide/tall"!
I just realised James is a shorty XD
Its unfortunate that Religion is viewed so negatively...however I adore the concepts and symbolism behind each character, I had not realized how poignant it all was. I am very excite for their adventures and am thrilled that this series is set during this time period!
I'm not anti-religion and plenty of my friends are religious, but I'm attempting to make a commentary on the corruption of religious superpowers and governments that I see a lot today, but using piracy and a different period as a more interesting setting. Using conquistadors and the Spanish inquisition was intentional, as those were some of the darkest moments of religion in history, in my opinion.
You are absolutely correct, the injustices of religion were record high in that time, and that's why I think your message works because it applies to the era. I am just personally sad for past mistakes and how corrupted institutions are currently, because humans are using it for their own motives. However I hope you know that there are still many who are actually living by the principals of their faiths Great story idea, I think this will be amazing!
I think I left a comment on here before, but I do not see it now so I shall type it again. First off, I love the character designs and how they reflect each unique personality. I am confused on one point, though. It is clear to me what Louis and James represent, but does Santiago symbolize religion as a whole or just when it is taken to extremes? Sorry if I am spamming your page.
I must say, I really love the characters and feel that they have a good, solid amount of depth to them. I am confused on one point, though. It seems to me from Santiago's description that he represents religion when it is taken to extremes rather than the pious practice of it. Am I correct or mistaken?


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The Intracellular Redox Stress Caused by Hexavalent Chromium
is Selective for Proteins that Have Key Roles in Cell Survival and
Thiol Redox Control
Judith M. Myers1, William E. Antholine2,3, and Charles R. Myers1,3,*
1Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701 Watertown
Plank Road, Milwaukee, WI 53226, USA
2Department of Biophysics, Medical College of Wisconsin, 8701 Watertown Plank Road,
Milwaukee, WI 53226, USA
3Free Radical Research Center, Medical College of Wisconsin, 8701 Watertown Plank Road,
Milwaukee, WI 53226, USA
Hexavalent chromium [Cr(VI)] compounds (e.g. chromates) are strong oxidants that readily enter
cells where they are reduced to reactive Cr intermediates that can directly oxidize some cell
components and can promote the generation of reactive oxygen and nitrogen species. Inhalation is
a major route of exposure which directly exposes the bronchial epithelium. Previous studies with
non-cancerous human bronchial epithelial cells (BEAS-2B) demonstrated that Cr(VI) treatment
results in the irreversible inhibition of thioredoxin reductase (TrxR) and the oxidation of
thioredoxins (Trx) and peroxiredoxins (Prx). The mitochondrial Trx/Prx system is somewhat more
sensitive to Cr(VI) than the cytosolic Trx/Prx system, and other redox-sensitive mitochondrial
functions are subsequently affected including electron transport complexes I and II. Studies
reported here show that Cr(VI) does not cause indiscriminant thiol oxidation, and that the Trx/Prx
system is among the most sensitive of cellular protein thiols. Trx/Prx oxidation is not unique to
BEAS-2B cells, as it was also observed in primary human bronchial epithelial cells. Increasing the
intracellular levels of ascorbate, an endogenous Cr(VI) reductant, did not alter the effects on TrxR,
Trx, or Prx. The peroxynitrite scavenger MnTBAP did not protect TrxR, Trx, Prx, or the electron
transport chain from the effects of Cr(VI), implying that peroxynitrite is not required for these
effects. Nitration of tyrosine residues of TrxR was not observed following Cr(VI) treatment,
further ruling out peroxynitrite as a significant contributor to the irreversible inhibition of TrxR.
Cr(VI) treatments that disrupt the TrxR/Trx/Prx system did not cause detectable mitochondrial
DNA damage. Overall, the redox stress that results from Cr(VI) exposure shows selectivity for
key proteins which are known to be important for redox signaling, antioxidant defense, and cell
© 2011 Elsevier Ireland Ltd. All rights reserved.
*Corresponding author: Charles R. Myers, Ph.D., Department of Pharmacology and Toxicology, Medical College of Wisconsin, 8701
Watertown Plank Road, Milwaukee, WI 53226 USA, email@example.com, phone: 414-955-8593, fax: 414-955-6545.
Publisher's Disclaimer: This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our
customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of
the resulting proof before it is published in its final citable form. Please note that during the production process errors may be
discovered which could affect the content, and all legal disclaimers that apply to the journal pertain.
NIH Public Access
Author Manuscript
Toxicology. Author manuscript; available in PMC 2012 March 15.
Published in final edited form as:
Toxicology. 2011 March 15; 281(1-3): 37–47. doi:10.1016/j.tox.2011.01.001.
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Chromium; Thioredoxin; Thioredoxin reductase; Peroxiredoxin; Bronchial epithelial cells
Hexavalent chromium [Cr(VI)] exposure can occur as a result of several industrial uses
including chromate (CrO42−) pigments, chromate-based corrosion inhibitors, stainless steel
machining and welding, chrome plating, and others. Inhalation is a common form of Cr(VI)
exposure, and results in a number of serious respiratory effects, including pulmonary
fibrosis, chronic bronchitis, lung cancer, and others (Baruthio, 1992; Deschamps et al. 1995;
Franchini et al. 1983; Ishikawa et al. 1994). The bronchial epithelial cells line the airways
and are therefore directly exposed to inhaled Cr(VI) fumes and dusts. Because industrial
uses result in the annual release of more than 105 tons of Cr to the environment,
environmental exposure is also of significant concern and Cr is a significant contaminant at
hundreds of sites (Environmental Protection Agency, 1999).
Cr(VI) compounds enter cells via an anion carrier, and once inside cells Cr(VI) is reduced to
Cr(III), the next stable oxidation state, by a variety of chemical and enzymatic intracellular
reductants (Borthiry et al. 2007; Myers et al. 2000b; Shi and Dalal, 1990; Standeven and
Wetterhahn, 1992; Suzuki and Fukuda, 1990). During this reduction, the reactive species
Cr(V) and/or Cr(IV) are formed, and these can directly cause oxidative damage (Sugden,
1999; Sugden et al. 2001) and generate reactive oxygen species such as hydroxyl radical
(HO•) via redox cycling (Borthiry et al. 2007; Shi et al. 1999a; Shi and Dalal, 1992; Shi et
al. 1999b; Standeven and Wetterhahn, 1991). Cr(VI) can also promote the generation of the
highly reactive oxidant peroxynitrite (Pritchard et al. 2000).
Cellular indicators of intracellular redox status demonstrate considerable oxidative stress as
a result of the reductive activation of Cr(VI). Cr(VI) treatment of human bronchial
BEAS-2B cells results in the pronounced inhibition of thioredoxin reductase (TrxR)1 and
the oxidation of the thioredoxins that are dependent upon TrxR for their reducing
equivalents (Myers et al. 2008;Myers and Myers, 2009). Peroxiredoxins such as Prx1 and
Prx3, which are directly dependent on their respective thioredoxins, are also oxidized by
Cr(VI) treatments that result in significant Trx oxidation (Myers and Myers, 2009).
Mitochondrial Trx2 is more susceptible than cytosolic Trx1 (Myers et al. 2008;Myers and
Myers, 2009) which implies that Cr-mediated pro-oxidant effects may be greater in the
mitochondria, or that the mitochondria may be more susceptible. Cr(VI) treatments that
cause Trx2 oxidation in BEAS-2B cells also cause pronounced and irreversible inhibition of
aconitase (Myers et al. 2010). Mitochondrial electron transport complexes I and II are also
inhibited, resulting in the appearance of electron paramagnetic resonance (EPR) signals that
are consistent with the disruption of electron flow through these complexes (Myers et al.
2010). The activities of complexes I and II and aconitase are known to be susceptible to a
number of oxidants including reactive oxygen and nitrogen species (Gardner et al.
1994;Pearce et al. 2001;Powell and Jackson, 2003;Williams et al. 1998;Zhang et al. 1990).
The thioredoxins are normally maintained largely in the reduced state in cells (Myers et al.
2008; Nordberg and Arnér, 2001; Szadkowski and Myers, 2008; Watson et al. 2003), and
1Abbreviations: AMS, 4-acetamido-4'-maleimidylstilbene-2,2'-disulfonate; BSA, bovine serum albumin; DHA, dehydroascorbate;
DTNB, 5,5'-dithiobis(2-nitrobenzoic) acid; EPR, electron paramagnetic resonance; Fe-S, iron-sulfur; HBSS, Hank's balanced salt
solution; IAF, 5-iodoacetamidofluorescein; MnTBAP, Mn(III)tetrakis(4-benzoic acid)porphyrin chloride; NEM, N-ethylmaleimide;
Prx, peroxiredoxin; Prx1, peroxiredoxin-1; Prx3, peroxiredoxin-3; Trx, thioredoxin; Trx1, thioredoxin-1; Trx2, thioredoxin-2; TrxR,
thioredoxin reductase; Txnip, thioredoxin-interacting protein; ZFS, zero field splitting.
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the TrxR/Trx system has a critical role in maintaining many intracellular protein thiols in
their reduced state (Arnér and Holmgren, 2000). The TrxR/Trx system is critical for normal
cell growth and viability and inhibition or suppression of these proteins enhances oxidant
sensitivity and decreases survival (Chen et al. 2006; Hansen et al. 2006; Nordberg and
Arnér, 2001). The peroxiredoxins are also important for cell survival (Chang et al. 2004;
Cox et al. 2008a, 2008b), and one or more peroxiredoxins may have critical roles in H2O2
signaling (Chang et al. 2004; Low et al. 2008). The effects of Cr(VI) on these proteins
therefore not only serve as indicators of intracellular redox stress, but could be important
contributors to Cr(VI) toxicity.
The purpose of the studies reported here was to further define the effects of Cr(VI) exposure
on the TrxR/Trx/Prx system. Several new findings are reported: (a) the thiols of the Trx/Prx
system are among the most sensitive of the protein thiols to Cr(VI) treatment; (b) the
oxidation of the Trx/Prx system also occurs in primary human bronchial epithelial cells and
is therefore not unique to the BEAS-2B cell line; (c) the effects of Cr(VI) on the TrxR/Trx/
Prx system are not altered by increasing the intracellular levels of ascorbate; (d) a
peroxynitrite scavenger does not protect the TrxR/Trx/Prx system or the electron transport
chain from Cr(VI); (e) Cr(VI) does not cause nitration of tyrosines within TrxR; (f) Cr(VI)
treatments that disrupt the TrxR/Trx/Prx system do not cause detectable mitochondrial DNA
damage; and (g) Cr(VI) does not alter the levels of Trx-interacting protein (Txnip).
2. Materials and methods
2.1. Chemicals and reagents
The following were purchased from Invitrogen Corp. (Carlsbad, CA): Hank's balanced salt
solution (HBSS), 4-acetamido-4'-maleimidylstilbene-2,2'-disulfonate (AMS), and pre-cast
gels (12% Bis-Tris, 16% Tris-Glycine) and matching electrophoresis and loading buffers.
Phenylmethylsulfonyl fluoride and Tris were from Research Organics (Cleveland, OH).
EDTA, guanidine-HCl, and trichloroacetic acid were obtained from Fisher Scientific
(Hampton, NH). Sodium chromate (Na2CrO4; 99+%) was the highest purity available from
Aldrich Chemical (Milwaukee, WI). Chromates are recognized carcinogens and should be
handled accordingly. Mn(III)tetrakis(4-benzoic acid)porphyrin chloride (MnTBAP) was
obtained from Axxora LLC (San Diego, CA). Primary antibodies were from the following
sources: Trx1 (Abcam 16835, rabbit polyclonal to full-length human Trx1), Trx2 (Santa
Cruz 50336, rabbit polyclonal to residues 92–166 of human Trx2), TrxR1 (Abcam 16840,
rabbit polyclonal to full-length human TrxR1), Prx1 (Abcam 58252, mouse monoclonal to
recombinant human Prx1), Prx3 (Abcam 16751, mouse monoclonal to recombinant human
full-length Prx3), GAPDH (Abcam 9485, rabbit polyclonal to full-length human GAPDH),
Txnip (also known as VDUP1, Santa Cruz 33098, goat polyclonal to a C-terminal epitope of
human Txnip), and nitrotyrosine (Santa Cruz 32757, mouse monoclonal to 3-nitrotyrosine).
Affinity purified horseradish peroxidase-conjugated secondary antibodies were goat-
antirabbit IgG (Promega W401B), goat anti-mouse IgG (Promega W402B), and donkey
anti-goat IgG (Santa Cruz 2020). Anti-Trx1 only detects cytosolic Trx1, not mitochondrial
Trx2. Anti-Trx2 reacts with Trx2 but not Trx1. In SDS-PAGE, anti-TrxR1 detects a 55 kDa
protein in human cell lysates (the expected size for the TrxR monomer) and reacts strongly
with purified TrxR. In reducing SDS-PAGE gels, anti-Prx1 and anti-Prx3 detect single
bands of ca. 21 and 25 kDa, respectively, consistent with their expected masses. In non-
reducing gels, they detect the dimeric (oxidized) forms of these peroxiredoxins in cells
under oxidative stress. Anti-nitrotyrosine reacts strongly with nitrated BSA but shows no
reactivity with BSA. All other chemicals and reagents were purchased from Sigma Chemical
or from the sources indicated.
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2.2. Cell culture and Cr(VI) treatment
BEAS-2B cells (human bronchial epithelial cell line, ATCC CRL-9609) were grown at 37°C
in humidified air containing 5% CO2 in Dulbecco's Modified Eagle's Medium with 25 mM
HEPES and 4.5 g/L glucose (BioWhittaker 12-709F), supplemented with 10% LHC-9
medium (Invitrogen), 10% fetal bovine serum (Valley Biomedical, Winchester, VA),
penicillin (100 U/ml), and streptomycin (100 µg/ml). The cells were fed every 48 h, and
were subcultured prior to reaching confluence using the Reagent Pak system (Clonetics,
CC-5034). Normal plating density was 3000 to 5000 cells/cm2.
Primary normal human bronchial epithelial (NHBE) cells (Lonza Biosciences CC-2641)
were grown in BEGM® medium (Lonza CC-3171) with the recommended BulletKit growth
supplements (Lonza CC-3170). They were fed every 48 h, and were subcultured as
described above. Normal plating density was 3500 cells/cm2.
Both BEAS-2B and NHBE cells were grown to 70–90% of confluence (typically in T-75 or
T-25 flasks), washed once in pre-warmed HBSS, and treated with Cr(VI) as Na2CrO4 in
HBSS at 37°C (see specifics below). Untreated cells were exposed to HBSS without Cr(VI).
Following treatment, the cells were washed in HBSS and harvested as described below for
the various assays. All experiments were repeated three times, except where noted
2.3. 2D Electrophoretic analysis of oxidant-sensitive thiol proteins
The analysis of oxidized protein thiols in control and Cr(VI)-treated BEAS-2B cells was
done using published methods (Baty et al. 2005; Cox et al. 2008b). BEAS-2B cells were
washed once in pre-warmed HBSS, and treated with 0 or 25 µM Cr(VI) as Na2CrO4 in
HBSS at 37°C for 90 min. Following treatment, the cells were washed and harvested into
HBSS, and pelleted by centrifugation. The cell pellet was suspended in buffer (40 mM
HEPES pH 7.4, 50 mM NaCl, 1 mM EDTA, 1 mM EGTA, and Roche complete protease
inhibitor cocktail) containing 0.1 M N-ethylmaleimide (NEM) to alkylate thiols. After a 15
min incubation at room temperature, 3-[(3-Cholamidopropyl)dimethylammonio]-1-
propanesulfonate (CHAPS) was added to a final concentration of 1% (w/v), followed by
vortexing and another 15 min incubation. Excess NEM was removed using BioRad Micro
Bio-Spin 6 chromatography columns. The eluate was treated with 1 mM dithiothreitol for 10
min to reduce oxidized thiols, followed by 0.2 mM 5-iodoacetamidofluorescein (IAF) for 10
min to label the resulting thiols. Unreacted IAF was removed using microspin
chromatography columns, and the samples were analyzed by 2D electrophoresis using the
ZOOM IPGRunner™ system (Invitrogen). The first dimension was a nonlinear pH 3–10
gradient (pH 4–7 occupies nearly all of the area) and the second dimension was SDS-PAGE
in 4–12% Bis-Tris gels with MOPS SDS running buffer. Images of the gels were captured
and analyzed using a Typhoon 9400 gel imager (GE Healthcare).
2.4. Redox blots for thioredoxins and peroxiredoxins
BEAS-2B cells were washed once in pre-warmed HBSS, and treated for 3 hr with 0, 25 or
50 µM Cr(VI) as Na2CrO4 in HBSS at 37°C. NHBE cells were treated similarly except that
they were treated for 3 and 6 hr. Given that the NHBE cells had not been studied in this
regard, the use of both exposure times provided for analysis of the effects of both
concentration and time. In some experiments with BEAS-2B cells, just prior to Cr(VI)
exposure, the cells were pretreated with vehicle or 0.2 mM MnTBAP for 2 hr, or with
vehicle or 1 mM DHA for 90 min. Following Cr treatment, the cells were washed once in
HBSS and immediately processed for redox western blots for Trx1, Trx2, Prx1, or Prx3 as
previously described (Myers and Myers, 2009) based on the principles in the original
protocols (Chen et al. 2006; Cox et al. 2008b; Halvey et al. 2005). Mitochondria were
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isolated from BEAS-2B cells and used as standards for oxidized and reduced Trx2 as
previously described (Myers et al. 2008; Szadkowski and Myers, 2008).
2.5. TrxR activity
BEAS-2B cells were washed once in pre-warmed HBSS, and treated for 3 hr with 0, 25 or
50 µM Cr(VI) as Na2CrO4 in HBSS at 37°C. In some experiments, just prior to Cr(VI)
exposure, BEAS-2B cells were pretreated with vehicle or 0.2 mM MnTBAP for 2 hr, or with
vehicle or 1 mM DHA for 90 min. Following Cr treatment, the cells were washed twice in
HBSS, scraped into 0.5 ml HBSS, pelleted by centrifugation (800 × g, 5 min) and frozen
(−80°C). The pellets were thawed, suspended in 0.1 M sodium phosphate pH 7.4/5 mM
EDTA and sonicated twice (15 sec each) on ice. Following centrifugation (30 min at 12000
× g), the supernatants were analyzed for TrxR activity at 37°C, measured as the NADPH-
dependent reduction of DTNB (5,5'-dithiobis(2-nitrobenzoic) acid) (Holmgren, 1977).
Incubation with DTNB (3 mM) during the first 5 min consumes non-specific thiols, after
which NADPH (0.2 mM) was added to the sample cuvet and the reduction of DTNB was
followed at 412 nm. The amount of NADPH-dependent activity that was inhibited by
auranofin (4 µM) is attributed to TrxR (Gromer et al. 1998).
2.6. Electron Paramagnetic Resonance
BEAS-2B cells were pre-treated with 0.2 mM MnTBAP or vehicle for 2 hr, and then 25 µM
Cr(VI) was added for 3 hr. The cells were then washed and harvested into HBSS, pelleted
by centrifugation (5 min, 100 × g), and the final cell suspensions (ca. 8 × 106 cells in 0.3 ml
HBSS in a 4-mm quartz EPR tube) were immediately frozen in liquid nitrogen (77 K) and
stored, typically for less than one week. EPR spectra were obtained at liquid helium
temperature (10 K) using a Bruker E500 ELEXSYS spectrometer (Silberstreifen, Germany)
with an Oxford Instruments ESR-9 helium flow cryostat (Oxfordshire, UK) and a Bruker
DM0101 cavity. Instrument settings are indicated in the results. EPR spectra were confirmed
in replicate experiments. The g values were determined by comparison to the 2,2-
diphenyl-1-picrylhydrazyl radical which has a g value of 2.0036.
2.7. Nitration of TrxR
BEAS-2B cells were washed once in pre-warmed HBSS, and treated with 0, 25 or 50 µM
Cr(VI) as Na2CrO4 in HBSS at 37°C for 3 hr. Following treatment, the cells were washed
twice in HBSS, scraped into 0.5 ml HBSS, and pelleted by centrifugation (800 × g, 5 min).
The pellets were resuspended in cold lysis buffer (20 mM Tris-HCl pH 7.5, 150 mM NaCl,
1% Triton X-100, 0.5% deoxycholate, 1 mM Na3VO4, 10 mM NaF, 12 mM β-
glycerophosphate, 5 mM EGTA, 1 mM phenylmethanesulfonylfluoride, Roche complete
protease inhibitor) and rocked at 4°C for 30 min. After passage several times through a 22-
ga needle, the lysates were clarified by centrifugation (15000 × g, 10 min, 4°C). The
supernates were incubated with 2 µg anti-TrxR (Abcam 16840) for 1 hr and then with
protein A/G agarose overnight (4°C). The agarose was pelleted (1000 × g, 5 min, 4°C),
washed three times in lysis buffer, mixed with loading buffer and reducing agent, boiled for
2–3 min, and aliquots of the supernate were run on SDS-PAGE. The lanes to be probed with
anti-nitrotyrosine antibody (Santa Cruz sc-32757) were loaded with 25% of the total
supernate, whereas those to be probed with anti-TrxR1 were loaded with 10-fold less (2.5%
of the supernate). Nitrated albumin (Sigma N8159) was used as a positive control for the
anti-nitrotyrosine antibody, whereas bovine serum albumin (BSA) was used as a negative
Myers et al.Page 5
Toxicology. Author manuscript; available in PMC 2012 March 15.
NIH-PA Author Manuscript
NIH-PA Author Manuscript
NIH-PA Author Manuscript


text:
Comment Re:The simplest solution... (Score 2) 447
"newbie_fantod rapes children and uses Apple products exclusively" -- 5 STARS
"newbie_fantod rapes children and uses Apple products exclusively" -- 5 STARS
1. As droughts become more common and severe, the price of meat (which takes a lot more water per calorie than veg) will rise. Alternatively, the animal rights folks will make strides that make factory farming illegal and thus forces all meat to be produced at small, organic/free range farms. Supply goes down, price goes up.
2. Meat substitutes will get tastier and tastier, and as demand increases, production will scale and prices will go down.
I'm a carnivore (and bacon-lover, especially), but I see this as a good thing.
There are certain roads I prefer to take and others I prefer to avoid, certain maneuvers I prefer to make and others I dislike. Example: especially if I'm navigating someplace unfamiliar, I'd much rather take the "least complicated" route that involves the fewest turns, especially if the time saving is less than 15 minutes.
Google Maps tracks this, both if I'm putting together the route on the computer (for printing out and taking with me) or if I'm actually navigating. And yet its suggested directions never change. It seems like there'd be MORE than enough data accumulated in a relatively small number of drives for GMaps (or Waze, is after all owned by Google, or whatever) to notice "Ah, this person hates taking non-protected left turns," or, "this person will not take the beltway for any more than a half-hour's time savings," and to adjust the directions it gives accordingly. They personalize search results. Why not directions?
Real Programmers think better when playing Adventure or Rogue.


text:
04/29/2014 - 11:19AM | by Matt Crane
07/31/2012 - 02:29PM | by Matthew Colwell
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One thought on "Senator Ted Cruz: National School Choice Week – 2/2/13"
I just have to comment that this guy is the most arrogant, spiteful, disrespectful Congress member in a long time and he is just getting started. He will be insufferable. Why can't the Republican party vote in serious minded, statesmanlike members into office?


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Bend there, done that
Despite being preoccupied by the fate of his beloved Chelsea, Page 2's Michael Davies found some time to break down the film "Bend It Like Beckham."
Updated: May 30, 2006, 4:50 PM ETBy Michael Davies | Special to Page 2
New York -- May 11th -- 9:30 a.m.Right now, I still can't review "Bend It Like Beckham" (perhaps the greatest women's sports movie ever made -- think about that for a second), an opening which is probably not going to please my incredibly patient editors at Page 2.
The fact is, I have procrastinated horribly since accepting the assignment to write a review of the word-of-mouth momentum-gaining chick flick three weeks ago (don't worry, I'm not going off all Charlie Kaufman ...
Read full story
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- Davies: The 'other' Open
- Davies Day 29, 30, 31: The End of Days
- Davies Day 27: Who wants to be a diary expert?
- Davies Day 26: Styling underarms and Brazilian bikinis
- Davies Day 25: Managing to Wade through
- Davies Day 24: Where God shines his light
- Davies Day 23: One more time on instant replay
- Davies Day 22: Football greats stole my cell phone
- Davies Day 21: Unanswered prayers
- Davies Day 20: Heaven, hell and a guy named Hector
- Davies Day 19: South Korea alters power structure
- Davies Day 18: Left at the altar
- Davies Day 17: Dawn of a new day for U.S. soccer
- Davies Day 16: The dreaded Niigata sickness
- Davies Day 15: Kids, don't do mingers
- Davies Day 14: A World Cup twisted from its roots
- Davies Day 13: Ending on a low note
- Davies Day 12: Fast train to nowhere
- Davies Day 11: It just keeps getting better
- Davies Day 10: Seeing red, white, blue ... and green
- Davies Day 9: Cheering for jolly old Nippon
- Davies Day 8: Nobody knows anything
- Davies Day 7: Soccer is the curse of the drinking class
- Davies Day 6: I've got your U.S. boys' backs
- Davies Day 5: Turning Japanese
- Davies Day 4: Satellite Stadium, take a bow
- Davies Day 3: Where's the passion?
- Davies Day 2: Ga-ga over the boys in green
- Davies Day 0 and Day 1: The 'other' football


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... think Brisbane is bad, you haven't seen anything. There are HUGE projects undertaken everywhere and I'm sure it'll look beautiful when it's all finished. We strolled through the Mall, then past the Governor's House, towards the Swan River.
A particularly interesting building caught our attention. It was the Swan Bell Tower, a quite unique and impressive structure. We booked a tour and admired the view of the Swan River and Kings Park from the 6th floor ...
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Page 1
J. Cell. Mol. Med. Vol 10, No 3, 2006 pp. 569-576
It is essential to know where we have been in order
to make reasonable predictions as to where we are
headed. This article represents one person's recol-
lection of the history of Tissue Engineering [1] and
consequently, it will most certainly contain some
personal biases. I apologize to any individuals
whose significant contributions to the field of tissue
engineering that may have not been presented here
as a result of my own misunderstanding or over-
sight. In presenting a historical perspective of the
emergence of tissue engineering as a multidisci-
plinary science, I will include the information con-
cerning the development of the journal "Tissue
Engineering" and the formation of the society, both
of which have evolved during the last decade.
Significant future challenges will be discussed.
The famous painting by Fra Angeliac entitled,
"Healing of Justinian" depicting the brothers Saints
Damien and Cosmos Transplanting a Homograft
limb onto a wounded soldier is often referred to as
the first historical reference to "tissue engineering".
However, Genesis I:1 "The Lord, breathed a deep
sleep on the man and while he was asleep he took out
one of his ribs and closed up its place with flesh. The
Lord God then built up into a woman the rib that he
had taken from the man" [2], may be interpreted as
the oldest written reference.
As recently as the 1980's, the term tissue engi-
neering was loosely applied to the use of prosthetic
devices and the surgical manipulation of tissues. In
actuality, a meeting held in the late 1980's in
Keystone, Colorado entitled, "Tissue Engineering",
sponsored by the National Science Foundation had
very little to do with the discipline of tissue engi-
neering as it is viewed today. While the meeting did
emphasize efforts to manipulate living tissues and
potentially combine them with prosthetic materials,
the actual generation of new tissue utilizing biolog-
ics, either alone or in combination with appropriate
scaffolding material was not a focus of the meeting.
The roots of Tissue Engineering, as a modern sci-
entific discipline, dedicated to the generation of new
tissue using the principles of engineering in combi-
nation with an understanding and application of the
biologic sciences, are deeply seated in Boston. To my
knowledge, the first recorded use of the term Tissue
Engineering, as it is applied today, was in a published
article entitled, "Functional Organ Replacement: The
New Technology of Tissue Engineering" [3] in
"Surgical Technology International" in 1991.
The early years
A pediatric orthopedic surgeon at the Children's
Hospital, W. T. Green, M.D., undertook a number of
experiments in the early 1970's to generate new carti-
lage using chondrocytes seeded onto spicules of bone
and implanted in nude mice. Although unsuccessful,
The history of tissue engineering
Charles A. Vacanti *
Harvard Medical School, Department of Anesthesiology, Brigham and Womens Hospital,
Boston, MA, USA
Received: May 24, 2006; Accepted: July 21, 2006
* Correspondence to: Charles A. VACANTI, MD E-mail: firstname.lastname@example.com
Tissue Engineering Review Series
Guest Editor: R. E. Horch
Available online at
Reprinted from:
Journal of Cellular and Molecular Medicine
• The early years
• Development of an organizational structure
• The society
• "Tissue Engineering", the journal
• Tissue engineering and the public arena
• Future challenges
• In conclusion
Page 2
he correctly concluded that with the advent of innova-
tive biocompatible materials it would be possible to
generate new tissue by seeding viable cells onto
appropriately configured scaffolds. Several years later,
Drs. Burke and Yannas of the Massachusetts General
Hospital and M.I.T. collaborated in studies in both the
laboratory and in humans to generate a tissue-engi-
neered skin substitute using a collagen matrix to sup-
port the growth of dermal fibroblasts. Dr. Howard
Green later transferred sheets of keratinacytes onto
burn patients, while Dr. Eugene Bell seeded collagen
gels with fibroblasts, referring to them as contracted
collagen gels. All of these examples represent seeds of
the new discipline now known as Tissue Engineering.
Possibly the key point in the birth of this emerg-
ing field was in the mid-1980's when Dr. Joseph
Vacanti of the Children's Hospital approached Dr.
Robert Langer of MIT with an idea to prospectively
design appropriate scaffoldings for cell delivery as
opposed to seeding cells onto available naturally
occurring scaffolds having physical and chemical
properties that could not be manipulated, thus result-
ing in unpredictable outcomes. Dr. Vacanti designed
and implemented extensive studies to generate func-
tional tissue equivalents utilizing a branching net-
work of synthetic biocompatible/ biodegradable
polymers configured as scaffolds seeded with viable
cells. Although the most cited manuscript describing
this new discipline may be the article published in
Science by Langer and Vacanti [4], the original arti-
cle describing the new technology was published a
full five years earlier in 1988 in Archives in Surgery
[5], as a keynote presentation given at the meeting of
the American College of Surgeons in 1988.
Hoping to explore and define the potential of this
new field, a number of centers have been organized in
the United States and Europe. While the vast majority
of these efforts are offshoots of those based in the
Boston area, several arose spontaneously. Among the
first significant efforts outside of Boston were the
development of the Pittsburgh Tissue Engineering
Initiative (PTEI) in the early 1990s organized by Peter
Johnson, the Cardiovascular Tissue Engineering effort
under the direction of Dr. Robert Nerem at Georgia
Tech, laboratories overseen by Drs. Antonios Mikos
and Larry McIntire at Rice University in Houston, and
an effort established at UMass Medical School by Dr.
Charles A. Vacanti. Outside of the United States, Dr.
Julia Polak, a pathologist and stem cell biologist in
London, spearheaded an effort in Tissue Engineering
at the Imperial College and organized a British-based
society that developed a loose association with the
Tissue Engineering Society (TES) that had previously
incorporated in Boston. In the mid-to late-1990's, Dr.
Una Chen began conducting tissue engineering and
stem cell studies in Giessen, Germany. Dr. Clemente
Ibarra founded laboratories for tissue engineering at
the National Institute for Rehabilitative Medicine in
Mexico City and organized the Mexican Tissue
Engineering Society. Dr. Wolfgang Pulacher opened a
laboratory for tissue engineering in Innsbruck at the
Leopold Institute. During this period, the creation of
laboratories and the development of a tri-state effort in
Germany, Switzerland, and Southern France were
spearheaded by Drs. Raymund E. Horch and
G.B.Stark at the University at Freiburg. Their efforts
culminated in the formation of a Tissue Engineering
Society in Germany, and ultimately, in Western
Europe. By the late 1990's Dr. R. Hetzer, a cardiovas-
cular surgeon at the University of Berlin, and Dr.
Christof Brelsch, a liver transplant surgeon in
Hamburg, established collaborations with the
Children's Hospital in Boston, as did a group at Kyoto
University headed by Dr. Koichi Tanaka.
In Asia, Dr. Minora Ueda of Nagoya University
established a large tissue engineering effort in Japan,
and organized the first meeting of the Japanese
Tissue Engineering Society (1997) in Nagoya. The
first Chinese tissue engineering effort, sponsored by
the Chinese government, was founded by Dr. Yi Lin
Cao in Shanghai. Another by Dr. Steven Kim in
Seattle, and at the University of Washington under
the direction of Dr. Buddy Rattner, and in Toronto,
under the direction of Michael Sefton
By the mid to late 1990's, tissue engineering
efforts including one at Yale University, established
by Drs. Chris Brewer and Mark Saltzman, were
springing-up in virtually every developed country in
the world and several privately funded ventures in
Tissue Engineering began to arise.
Development of an organizational
In 1994 there was felt to be sufficient momentum to
organize a society and to establish a journal dedicated
to scientific interactions and the communication of
high quality scientific presentations and publications.
Page 3
The society
The Tissue Engineering Society (TES), conceived of
and founded by Drs. Charles A. and Joseph P. Vacanti
in Boston in 1994, was officially incorporated in the
state of Massachusetts on January 8, 1996. The origi-
nal Governing Board of seven members included the
founding Presidents, Drs. Charles and Joseph Vacanti,
as well as Dr. Robert Langer of MIT, Dr. Joseph
Upton of the Beth Israel Deaconess Medical Center
and Harvard Medical School, Dr. Tony Atala of
Children's Hospital, Mark Randolph of the
Massachusetts General Hospital and Linda Cina of
MIT. It was decided that the Society was to be an
international Society and that meetings would initial-
ly be held on a biannual basis. Over the next decade,
in conjunction with the Asian and European Societies,
TES would evolve and reorganize to become TESi
and then TERMIS, the Tissue Engineering and
Regenerative Medicine International Society, by
2005. Dues for membership in the original Society in
1996 were $40 for physicians and research scientists
and $15 for residents and students. Peter Johnson and
David Smith were instrumental in helping launch
TES, with David providing legal counsel, gratis. To
date, eight meetings have been held. Initially the
meetings were bi-annual with the first three being
held in Orland. Subsequent meetings were held at
various international locations on an annual basis.
The inaugural meeting of the international Tissue
Engineering Society (TES), organized by Charles and
Linda Vacanti, was held in December, 1996 at the
Lake Buena Vista Hotel in Orlando, Florida.
Attendance was approximately 300 people, represent-
ing thirteen countries. At the end of the first meeting,
the founding President, Dr. Charles A. Vacanti was
elected as the first elected President of the Society.
The next two meetings of the TES were also held at
the same location in Orlando, renamed the Wyndham
Hotel in 2000. Initially, somewhat of a "Mom & Pop"
organization, the inaugural meeting was organized to
a great extent by Linda K. Vacanti, who also hosted
the first two meetings and assumed responsibility for
all the administrative functions. All submitted
abstracts were read and evaluated Drs. Charles and
Joseph Vacanti. Subsequent meetings held annually
in different locations had increasing professional
input and organization. Successive elected presidents
of the society were, Joseph P. Vacanti, MD, of Boston
(the other founding father) in 1998, Peter Johnson,
MD, of the Pittsburgh Tissue Engineering Initiative in
2000, Robert Nerem, Ph.D. of Georgia Tech in 2002,
and Alan Russell of University of Pittsburgh in 2004.
As previously mentioned, Drs. Horch and Stark
of Freiburg, Germany encouraged the formation of
a European tissue engineering society (ETES) with
the assistance of Dr. Julia Polak of the Imperial
College in London and founder of the British
Society, and Dr. Ranieri Cancedda of Genoa, Italy,
who had spearheaded the formation of an Italian
society. The following year, 2001, the TESi meeting
was held in Freiberg, Germany, in combination with
the fledgling ETES. At the meeting hosted by Drs.
Stark and Horch, Dr. Cancedda was elected
President of the ETES. Subsequently, the European
Society, loosely associated with the London-based
British Tissue Engineering Society, and aligned
with the TESi, ultimately as a European continental
branch in a similar fashion to the Asian continental
branch of the TESi.
The Japanese Tissue Engineering Society was
organized in 1977 a few years after that of the the
international (TES) by Dr. Minoru Ueda with its first
meeting held in Nagoya, Japan. Dr. Yi Lin Cao then
formed the Chinese Tissue Engineering Society and
Shanghai-based Tissue Engineering Center. These
societies formed a loose association, and aligned as
the Asian branch of the international Tissue
Engineering Society, then referred to as TESi.
In 2002 the meeting of TESi was held in
Kobe, Japan in conjunction with the Japanese
Tissue Engineering Society (JTES) and the Asian
continental branch of TESi. Dr. Ueda, president
of both the JTES and the Asian branch of the
TESi hosted the meeting.
As a consequence of the SARS epidemic, the
2003 meeting of TESi, originally planned to be held
in Toronto, was moved to the location of the first
three TES meetings; that is, Orlando. The meeting,
hosted by Dr. John Davies and other scientists from
Toronto, was presided over by the fourth elected
President of now the TESi, Dr. Robert Nerem of
Georgia Tech. Dr. Alan Russell from the University
of Pittsburgh and the Pittsburgh Tissue Engineering
Initiative was elected at that meeting as the fifth
President of the international Society. Adecision was
made by the Governing Board of TESi to re-empha-
size that the future direction of the Society should
reflect its original intent. The Society, originally
formed as an international Society, founded in North
J. Cell. Mol. Med. Vol 10, No 3, 2006
Page 4
America, would have worldwide representation. It
would continue to support international meetings as
well as coordinate meetings in North America.
Following the meeting, a recommendation was made
to explore a more formal merging of what had been
continental branches of the former TESi. To reflect
the evolution of the discipline, which had expanded
to include regenerative medicine, the merged entity
was renamed the Tissue Engineering Regenerative
Medicine International Society (TERMIS).
In 2004 the meeting was held in Lausanne,
Switzerland. The following year, Dr. Yi Lin Cao host-
ed the largest TESi meeting to date, in conjunction with
the Chinese Tissue Engineering Society, in Shanghai,
China. More than 600 international attendees partici-
pated with more than 900 registered attendees.
In June of 2005, it was announced that ETES was
being recognized as a formal continental chapter of
the Society. Dr. Russell, the 5thPresident of TESi,
continued as President of TERMIS for an anticipated
two years. It was agreed that he would be followed
by Dr. Jöns Hilborn, who had been President of the
European Continental branch (ETES), as the sixth
President of the mother Society. A similar approach
is now being explored for what is currently the Asian
continental branch. The ultimate goal remains the
same; that is, to achieve a worldwide organization, as
originally envisioned.
Ten years after being established, the structure of
the society is certainly more well-defined, more effi-
cient as a business, and has a more sophisticated sys-
tem of governance. Its name has been changed to
reflect a broader scope. In spite of these changes, its
goals appear to be consistent with those initially
defined a decade ago by the founding board. The
society was to be an international society that would
"continually encourage and promote the exchange
of information in the field of Tissue Engineering
through education, research and the dissemination
of information useful to the individual and benefi-
cial to mankind". It seems as though we have come
full circle to where we started in 1996.
The journal
With the formation of the Society, the founding
Board of Governors felt that it was important to have
an effective means by which to exchange scientific
information and freely express new ideas. The jour-
nal "Tissue Engineering" was founded in 1994 by
Drs. Charles A. Vacanti of the Massachusetts General
Hospital and Harvard Medical School, and Antonios
Mikos of Rice University. Its Editorial Board was
composed of an international balance of physicians
and scientists, and its administration on a daily basis
was largely done by Linda K. Vacanti.
Initially, manuscript submissions were aggres-
sively solicited. In spite of the large, formal edito-
rial board, a high percentage of the original
manuscripts were reviewed by Drs. Mikos and
Vacanti. For a decade, Linda Vacanti and Carol
Lofton dedicated a tremendous administrative
effort to organizing the structure of the journal,
chasing-down and "hounding" reviewers for com-
ments, keeping authors informed of the status of
their manuscripts and proof-reading all of the arti-
cles. It was a thankless job, for which I would now
like to publicly thank them. Their dedication, judg-
ment and effectiveness have been stellar and the
journal owes much of its success to their efforts.
Mary Anne Liebert Publications has produced a
very professional, extremely well-managed journal,
and has been a delight to work with. Over a period
of ten years, the journal has grown in stature and
respect to now command an international audience
and an impact factor of greater than 3.
I would like to thank Patrea Pabst for her dedi-
cation in writing patent updates for the journal over
an extended period of time. Her articles have been
interesting, insightful and timely.
Michael Lysaght, Ph.D. has also generously pro-
vided legislative and business updates, for which I
would like to express my gratitude.
In 2000, the editorial board was modified as edi-
torial offices were added in London and Tokyo. Dr.
Julia Polak and Dr. Yasuhara Noishiki were named
Associate International Editors in the London and
Tokyo offices respectively. In 2003 Minora Ueda
replaced Dr. Noishiki and, in 2004 Dr. Teruro
Okano was named as the Asian Associate Editor. At
the end of 2003, Dr. Vacanti stepped down as a
founding editor. Dr. Peter Johnson then assumed
responsibilities as co-editor of the journal with Dr.
Mikos. The membership of the editorial board con-
tinues to evolve to allow input from young, emerg-
ing scientists. I would like to acknowledge and
thank Dr. Johnson for his efforts on behalf of the
journal and the Society. I would also like to express
Page 5
my tremendous admiration and thanks to Antonios
Mikos. My co-editor for almost a decade, who con-
tinues to serve as editor of the journal. I could not
have worked with a more reasonable, delightful,
organized, dedicated and objective person.
To date, the journal has retained the original name
"Tissue Engineering", which I believe accurately
reflects the scope of this specialized, multidisci-
plinary field. Significant efforts now exist to merge
the fields of tissue engineering and regenerative
medicine, as evidenced by the changes in name and
scope of the Society. Since both fields represent mul-
tidisciplinary efforts in medicine with many area of
overlap, arguments to merge them seem obvious. I
do however believe that tissue engineering is a sci-
ence devoted to the generation of new tissue by
J. Cell. Mol. Med. Vol 10, No 3, 2006
Fig. 1aScanning electro micrograph of cells seeded onto synthetic scaffold composed of a random array of polyg-
lycolic acid fibers.


text:
BEST WESTERN Hotel Esplanade
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Just after the turn of the century, Sweden experienced difficult economic times. Her family was struggling to support the children. At only 14 years old, Signe and a younger brother had to leave the family. They set off for America all by themselves. They had a sponsor in Iowa who would take them in and give them a job until they got established. The records at Ellis Island indicate ...
Thursday 8th May - SCHOOL
This was a school day and I spent the day in our partner school in Jakobsberg. The Swedes are very fond of their coffee and a classy coffee machine in the staff room is a welcome friend.
Schools in Sweden are not too different from those in the UK but the different climate means that ...
Ikea and Swedish Meatballs... In Sweden
This past weekend, I took the short 2.5 hour flight over to Sweden, to spend some time with Katelyn visiting a friend from Laurentian, Caroline, who is spending her semester studying in Vasteras, Sweden .
I arrived in Stockholm just after 2 o'clock. I had 4 hours until I had to be at the airport to meet Katelyn before heading to Vasteras. With all this free time, I decided to walk around the city. This is the first time since ...
Home Base
After Dublin, Västerĺs seems lacking, but it is back to classes until the next trip. This would be a good place to add that I have never felt unsafe in this city, even walking late at night or early in the morning. There are no neighborhoods that I have found that are not safe. Public transportation is also user friendly, although I have heard that this is not the case ...
Different life
... during our stay in Västerĺs, they suggested some fun activities on ice also, something like going fast with snow mobile or motocross bike, which on ice was totally amazing and of course gave adrenalin for one week ahead!
I should mention Turkish guy's Arsen dinners at his place in Top Tower (student accommodation)! He was always so creative and made it good. He never mided how many people are coming and he was always glad to feed everyone. Well, and once ...


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Imperial Hotel
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Travel Blogs from Davao
Young Adult Leadership Training
... correctly, we have 30 young adults in attendance from all three of the major regions of the Philippines. We had 18 young men and 12 young ladies. A few had to withdraw at the last minute due to work or family needs. Here's the figures: 18 young adult men; 12 young adult women for a total of 30 young adults. When we add the 12 pastors/leaders/wives, we had 42 total in attendance. Mr Baker, the Filipino pastors and the ...
Visiting Life Inmates Jed Sy and Jong Pilapil
... will provide on for each We're arranging for them to be purchased here in the Philippines, This will give them great encouragement as they can communicate with and be connected to the outside as they wait through their appeal process.
We also stopped by a nearby location where the United Church of God can be given a parcel of land to build a church building. There is a separate congregation here that has about 30 in attendance. We talked about ...
Tuburan - a well spring of educational inspiration
... locally and organizing parent education classes about how to raise spiritual children in a materialistic world. They were battling the bureaucracy of registering a school. They were gaining further educational qualifications to supplement their government recognized teaching qualifications, paying from their own pockets to achieve these goals ...
Vacation - With Chell
... 3-4 hours driving smoothly on the national highways, we reached Zoobic Safari by 11..
Zoobic Safari is a an amusement park offering a pleasant experience to know the exotic
animals roaming and frolicking in their natural habitat.
The Zoobic park, housing a plethora of animals and birds... the savannah, farm for exotic animals, the serpentarium, bringing us closer to a huge variety of reptiles, the croco loco, with over 200 crocodiles, the Aeta trail and show...a ...
Highway Vistas
... an villagers, is using its nose to choose the ripest and sweetest coffee cherries containing the beans that after fermenting in the animal's stomach, this natural process is giving the coffee its unique taste and aroma. The beans are gathered very early in the morning usually before the sun rises. As you leave this small but booming city behind, the journey continues on a straight section of this part of the Maharlika Highway, here you can actually see for miles well ...


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Hidden camera captures female with pierced tits and tat soaping up and lathering in a public shower
Woman is secretly video taped while taking a hot, steamy shower. Watch her suds up and have a front row view to her nipple rings.
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All or Nothing Trivia Answers for October 19th!
by Kris Valentine
posted Oct 19 2009 5:12PM
Q: According to a Harris poll, what are Americans fed up with the most? (a) Telemarketers (b) Automated Phone Systems (c) Junk Mail
A: 59% - junk mail (54% - don't want to press one; 49% detest telemarketing)
Q: WhichU.S. city ranks #1 in the number of miles driven per capita? a) Los Angeles b) Atlanta c) Houston?
A: Atlanta
Q: On average who laughs more, the average man or the average woman?
A: Man - 69 times a day (Woman; 55)
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Response to These Sorority Girls On A Selfie Spree At A Baseball Game Made People Really Mad For Some Reason:
So people in our generation take selfies? And your point? Shit I'd be taking selfies too if I didn't hate the way I looked. Take your pictures while you're young and beautiful
- Xtonicray thinks "For Anyone Who's Been Stabbed By A Pe..." is lol
You're sounding very holier than thou and very "I'm not like those other kids" and somehow you think it makes you better than other people. my point is you're sounding annoying and childish
Oh my god braces were so easy for me. Sure I had pain for like half a day after a tightening but tbh not even that bad. "Flossing " I did that like a month and I was like fuck it. Lol "forbidden food" I ate a lot of those , well I never bit into a whole apple I'm not an idiot . And my retainers are these clear molds of my teeth that don't make me look like an idiot sure they do give me slight lisp but whatever
You know what's even worse than that? People would vote for trump
I'm already serving it … Then again I'm in a licensed store … I'm not sure how I feel about the taste now that there's pumpkin in it
- Xtonicray thinks "Sorry To Ruin Your Day, But Starbucks..." is lol
Oh my god a collarbone! The horror! Has she no respect for herself?!? Think of the children! And the gross 15 year old boy who will apparently jizz his fucking pants if he sees some collarbone
it's not a male role, it's just that the Doctor just so happened to have regenerated into a white human male for as long as the show has been on. The doctor can regenerate into a freaking Silurian if he wanted but I would rather much prefer a human, male or female, and I would honestly still love the show just the same if it was somebody of color or a woman
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Obama shoots $17 billion out of $18 billion worth of presidential skeet discs.
CAMP DAVID, MD - The White House has released photos, some video and now statistics in reference to the President's skeet shooting. In his most recent 90 minute outing, President Obama successfully shot a very accurate $17 billion out of $18 billion worth of presidential sport clays.
Video has been released showing the skeet disc carnage left behind from the President's most recent outing. A small pile of shattered discs litter the grounds of Camp David, though approximately 15 minutes worth of the discs remain intact and proudly displaying the Presidential seal. The value of the remaining discs is estimated to be one billion dollars.
"People were complaining that I did not understand the mindset of gun owners," said President Obama. "Well, I think this proves that I do. I understand the thrill of pulling the trigger of Shotgun 1, of holding the cold grey steel, and the exuberance of hitting my target. As an added bonus, I also know the awesomeness of ordering a drone attack on all of the targets I missed."
When asked about the cost of the Presidential skeet discs, the White House Budge Office was unconcerned. "Presidential discs cost about $2,200,000 a piece and fall under the Presidential sport and hobby section of the budget," said senior economic advisor David Oakes. "Adjusted for inflation, President Obama has spent half as much money on skeet shooting as President Eisenhower spent on lawn jarts."
- Donald Trump admits to killing puppies, rises 6 points in polls
- Meghan Kelly begins preparing debate questions for Kanye West
- John Dowd alleges Pete Rose was actual Nicole Simpson killer
- Donald Trump preparing to fire all other Presidential candidates during debate
- UC Police outraged by Zimbabwe lion shooting
- Presidential mini-golf game ruins childrens birthday party
- John Kerry's family tree offers reason for long face.
- 295 million Americans feel they were unfairly targeted by IRS
- Obama offers to trade Geraldo Rivera for jailed American reporters in North Korea
- WikiLeaks Founder prison escape plans leaked to law enforcement
- Osama Bin Laden under investigation for misreporting actual costs of terrorist operations


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First series since 2006
With the Tigers in the World Series for the first time since 2006 and with a chance to win their first title in more than a quarter-century, residents of Detroit and its suburbs are experiencing a full-blown case of baseball fever. AP video. (Oct. 23)
Newsday.com now uses Facebook for our comment boards. Please read our guidelines and connect your Facebook account to comment.
Cosmos vs. Atlanta highlights Tom Cutinella anniversary vigil on football field Viktor Postol: One on one with HBO Lucas Matthysse: One on one with HBO Cutinella's parents on anniversary of son's football-related death Islanders' future bright in Brooklyn Jets QB Ryan Fitzpatrick on Brandon Marshall Raw: Richard Petty Revs Up Ben Carson's 2016 Bus HBO's Lederman previews Matthysse vs. Postol Raw: Wife of Dolphins player arrested at stadium Cosmos vs. FC Edmonton highlights Highlights: Farmingdale vs. Baldwin football


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First baseman Josh Banuelos, outfielder Amalani Fukofuka and relief pitcher Tripp Davis will represent the Idaho Falls Chukars as reserves at the first Pioneer League/Northwest League Midsummer Classic, scheduled for Aug. 4 in Spokane.
Banuelos has been a force at the plate, leading the Pioneer League with 34 RBIs. Banuelos, of Hanford, Calif., is hitting .338 with 47 hits.
Fukofuka's name is sprinkled throughout the list of Pioneer League leaders. The Hayward, Calif., native is hitting a team-high .350, good for fourth in the league. He is second in the league with 70 total bases, tied for second with 50 hits, tied for third with 12 doubles and tied for fourth with four triples.
Davis has been a bright spot on the mound, leading the Chukars with two wins while posting a 4.05 ERA in eight appearances. He had an 0.71 ERA in his first 16.2 innings.
Alexis Rivera, who started the year in Idaho Falls, also was selected to the All-Star squad but has since been promoted to Single-A Lexington and won't play in the game. When he left the Chukars three weeks ago, Rivera led the league in home runs (7), RBIs (17) and OPS (1.233). His home run total is still tied for third in the league.
Orem placed three players on the Pioneer League starting lineup (catcher Taylor Ward, third baseman Michael Pierson and outfielder Kyle Survance) and one more reserve. Missoula led the way with five selections.
For a full list of the All-Stars, visit http://www.milb.com/news/article.jsp?ymd=20150727&content_id=139048830&fext=.jsp&vkey=news_l128&sid=l128.


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All hotels in Edinburgh, Scotland, United Kingdom
1 room, 2 adults
21 Upper Gilmore Place, Edinburgh, Scotland, EH3 9NL, United Kingdom,
Amaryllis Guest House - Edinburgh
Lowest priceRs4,843
Best Price Guarantee
City-center guesthouse within walking distance of King's Theatre
- Free WiFi
Main amenities
- 5 guestrooms
- Breakfast available
- Multilingual staff
- Limited hour front desk
- Tour/ticket assistance
What's around
- In Bruntsfield
- Edinburgh International Conference Centre (0.5 mi / 0.9 km)
- King's Theatre (0.3 mi / 0.5 km)
This was a great B & B and location. Our room was very large and comfortable. Our hostess…
It was a great stay at nice B&B. A wonderful lady took care of us and watched out for us…
TripAdvisor Traveller Rating: 4
From 42 reviews
Enter dates to check prices
Hotel Info: from Rs4,843
- Double Room, Ensuite
- Family Room, Ensuite
- Twin Room, 2 Single Beds, Ensuite
- Triple Room, Ensuite
- Single Room, 1 Single Bed
Recommended hotels
At a glance
Key facts
Hotel size
- This hotel has 5 rooms
- This hotel is arranged over 3 floors
- Check-in time noon-9 PM
- Check-out time is 10 AM
Required at check in
- Credit card or cash deposit required
- Government-issued photo ID required
Travelling with others
- Pets not allowed
- Free WiFi in public areas
- Free WiFi in rooms
Nearby Three Star Edinburgh hotels
In the hotel
Food and drink
- Continental breakfast daily (surcharge)
Things to do
- Golfing nearby
- Front desk (limited hours)
- Tours/ticket assistance
- Year Built 1806
In the room
Home comforts
- Coffee/tea maker
- Iron/ironing board (on request)
Freshen up
- Private bathroom
- Shower only
- Hair dryer
Be entertained
- Flat-screen TV
- Cable TV channels
Stay connected
- Free WiFi
Top Edinburgh hotels
Recently reviewed hotels in Edinburgh
- Hilton Edinburgh GrosvenorFrom
- Ibis Edinburgh Centre South BridgeFrom
"Excellent hotel and location, walking distance to all sights in this beautiful city! Good value for money if you book in advance, cheers."Aug 3, 2015
- Ibis Edinburgh Centre Royal MileFrom
"The hotel is very central to all the action and close to the historic buildings in the city. In addition it is a 7 minute walk from the Queens train station. The service staff are…"Jul 30, 2015
- Tune Hotel - Haymarket, EdinburghFrom
"Perfect location Good hotel"Jul 14, 2015


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Cats Imitating Art
Here's a cute collection of famous artworks and cats striking the same poses. I found "Romeo And Juliet" by Ford Madox Brown particularly appealing, but you can see 21 such comparisons at Buzzfeed. Link
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Staples Inc.
The Staples App brings easy to your Windows 8 tablets, laptops and desktops. So go ahead – redeem coupons and rewards. Compare product prices and features. Check product availability at Staples stores near you. View your order history, update your shopping lists and find the ink and toner you need. And above all, add some easy to your workday.More
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1-10 of 30 reviews
Update fixed many issues!
The recent update as per this review's date, fixed a lot of issues, and the app has better performance, and is quicker to load results. Some of the description include html code that is being displayed as well, many images are blurry (low resolution images).More
- Yes (0)
- No (0)
Nice app
Nice app. The only dumb thing about it is that it will sign you out periodically. Seems like a big point to having an app is for it to save your login info...More
- Yes (0)
- No (0)
This is one of the best-done shopping apps that I've seen, and other devs should take lessons in ease-of-use and design. Most apps, I'd rather use the website. With this one, I haven't been back to Staples.com since I installed. It's easy, and a joy to use!More
- Yes (0)
- No (0)
Fantastic application !
So well designed, easy and cool navigation. Not too much serious apps for Windows RT like this one.More
- Yes (0)
- No (0)
Good job Laurent!
I like the performance. It's fast and easy to browse products of interest.More
- Yes (0)
- No (0)
Wow what a cool app!
Works great, smooth and allows me to shop. What a better experience than using the full website! Keep up the good work and keep adding more features!!More
- Yes (0)
- No (0)
Great app!
Staples made it easy!More
- Yes (0)
- No (0)
Not available anymore!
It disappear now!More
- Yes (0)
- No (0)
Great app
Simple to view use and get the most relevant information that I need to both shop online and in store without getting lost in a larger site. Nice job!More
- Yes (0)
- No (0)
Keep getting errors
Can't redeem rewards or place orders. Uninstalled.More
- Yes (0)
- No (0)
1-10 of 30 reviews
Staples Inc.
- Easily browse, search and buy from the entire Staples catalog
- View and use your rewards checks and get personalized offers
- Easy and direct access to the printers you use so that finding the ink and toner is easier than ever before
- Compare products side by side to make purchasing the right product easy
- Browse the Weekly Ad to find deals in your local store
- Find stores near you and check product availability
- Build and save lists for easy ordering and reordering
- Staples Windows 8 was built to provide an enhanced shopping experience on a touch medium like tablets and convertible desktops while also optimizing for a mouse and keyboard control
App details
- Publisher
Staples IncMore
- Category
- Approximate size
4.53 MBMore
- Age rating
For ages 12 and upMore
- Supported processors
arm, x64, x86More
- Supported languages
English (United States)
- Learn more
- Additional terms
- More
- Capabilities
Use your locationAccess your Internet connectionAccess your Internet connection and act as a server.Access your home or work networks
Version notes
- New Features - Printer Finder: Let the application search for your printer ink & toner with the touch of a button. - New navigation: Enhanced navigation path where you can browse more intuitively into thousands of products. - Performance Enhancements - Better search - Better loading times - Other performance improvements - Additional note: If for some reason you do not see the upgrade button in the Windows Store, please uninstall and reinstall the application.


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Shah Deep International Business Solutions Ltd. — shahdeepinternational.com is a Fraud Company
13 Reviews
dineshdod on May 21, 2012
This is a fraud company... i given one project to them on 10th month of 2011, and the project timeline is one month...but till today they are not responded and not given my project... and there is no proper contact and responsible persons...
i spoke with ASTI, NEHA AGARWAL, and MUKESH every time they will give promise to complete the work and they dont stand on that promise...
those asti and neha will talk very non sense and even they dont know anything technically... if we guide them they dont understand...
i tried to contact their officials they are more idiots then these people... they also dont stand on there words...
they dont have proper resource...
i lost my time, my money... and finally thinking to knock the door steps of honorable court...
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quick linksCURRENT ISSUE PREVIOUS ISSUES NEWS INDUSTRY NEWS COLUMNISTS BROWSE EDITORIAL TOPICS WEBINARS
resourcesABOUT MT AUTHOR GUIDELINES CLASSIFIEDS EDITORIAL CALENDAR MEDIA GUIDE MASSAGE MART SCHOOLS & EDUCATION FEEDBACK
Modernization of Chinese Medicine
Language – written, spoken, signed, or otherwise is learned as a means to express our individualized perceptions about the world around us. Language is designed to communicate our personal experiences.
Fish Oil: A Key Component of Positive Clinical Outcomes
Patients seem to be presenting with more complex problems, and many are responding to care more slowly or have completely unexpected results. Why?
Dorsiflexion Dysfunction: Evaluation & Manipulation Techniques
Almost every condition from the foot to the hip can be attributed to the inability to dorsiflex the ankle mortice and other joints that participate in dorsiflexion. Let's start by understanding normal versus abnormal dorsiflexion.
Oriental Medicine on the World Stage
"Let me win. But if I cannot win, let me be brave in the attempt." This simple, yet powerful statement was lived out time and time again by so many of the athletes from around the world during the Special Olympics World Games in Los Angeles.
The New Age of Communication
In the age of technology, everyone, including the patient, is seeking faster, easier ways to communicate. With a wealth of social media, blogs, websites and videos, we are constantly barraged with information – to the point of overload.
The Food Conversation: Nutrition and Your Practice
It's morning and your first patient rolls in with a triple espresso steaming in one hand and a frazzled, desperate look in her eye. "You gotta help me, doc, I am constipated unless I drink one of these, and I am exhausted and anxious all the time."
News in Brief
Call for Abstracts Announced - Parker Las Vegas 2016; Logan Adds Doctorate Degree; New Role for Dr. James Edwards.
Harvard Health References Flawed AHA Position Paper
In its special health report, "Stroke: Diagnosing, Treating, and Recovering From a 'Brain Attack,'" Harvard Health Publications includes information from the American Heart Association's 2014 position statement on cervical manipulation and cervical dissection – a statement the American Chiropractic Association emphasized in a letter to Harvard Health mixes "scientific facts with half-truths."
Fertility and Poly-Unsaturated Fatty Acids
Starting or expanding one's family is a major milestone. It's something that more and more people seek out health care advice and support for.
Healing Trauma: Cultivating Resilience and Presence Through Mindfulness, Part 2
In the last issue of Acupuncture Today, the first part of this article introduced the topic of trauma and resilience, and their relationship to the autonomic nervous system response and the concept of the spirit being grounded in the body, and suggested the importance of mindfulness as a tool for healing.
Help: A Need at Every Level
One of the great gifts of training in acupuncture is the ability to take good care of oneself. I recently had a bout of frozen shoulder — an inflammatory syndrome which can be debilitatingly painful and take years to resolve.
Patient-Centered Care vs. Payer Restrictions: Your Ethical Obligation
Do you have an ethical obligation to evaluate your patients, make a diagnosis and provide evidence-based, patient-centered health care, irrelevant to the payer restrictions?
The Short Leg Dilemma
When evaluating a new patient, it is common to note a relative shortening of one leg to the other. Some patients will even tell you they have one, and then pull out the store-bought heel lift they read about online.
The Zen Art of "One Point"
We were always told in our Zen Shiatsu training (by Japanese and Japanese American instructors) that our ultimate aim was to to find that "One Point." To be so focused we could touch just one point to transform Qi throughout a client's body.
Acupuncture Rising: From Acupuncture Anesthesia to Assisted-IVF, Part 1
Acupuncture's cultural and historical roots go back to the emergence of Chinese civilization. For more than 2,000 years, acupuncture needling has been continuously practiced on the largest population in the world.
Getting a YES: An Effective Strategy for Overcoming Patient Objections
Patients make more excuses for declining care from an acupuncturist than perhaps any other type of doctor. Various reasons hold them back from making a commitment to care.
More Chiropractors Required
An intriguing study published in the Journal of the American Board of Family Medicine examines how "chiropractic care affects use of primary care physician (PCP) services."
Change Lives by Supporting Chiropractic Research: Are You In?
The Palmer Center for Chiropractic Research (PCCR), in celebration of its 20th anniversary, has announced it is spearheading a fund-raising campaign to support chiropractic research.
An Acupuncturist's View of Medicinal Marijuana
The use of cannabis for medical purposes is very controversial. Use as a panacea by physicians uninitiated to the proper application of herbal medicine, as well as an excuse for recreational use have greatly confused the issue.
Practicing with Authenticity
To extrapolate from the above quote, patients love healthcare providers they can trust. One way to earn the trust of your patients is by practicing with authenticity. What does that mean, exactly?
Surprising Reasons for Orthotic Efficacy
Clinical outcome studies show orthotics are effective in the management of a wide range of injuries, including plantar fasciitis, Achilles tendinitis and patellofemoral pain syndrome.
Practice Policy (Gone Bad): The Sign
Every once in a while, you see something and think to yourself, That's a really bad idea. Case in point: I went to see my medical doctor the other day. Just after being "roomed," as they say, the nurse checked my vital signs. Then she left.
Nuts Reduce Risk of Heart Disease, Cancer and Other Health Problems
Several recent studies suggest regular consumption of nuts may provide a significant degree of protection against certain types of cancer, heart disease, possibly type 2 diabetes and some neurodegenerative diseases.
A Chiropractor's Guide to Yoga
"Doctor, can I continue to do yoga while undergoing your care?" "Is it OK for me to go back to yoga while I'm getting my back treated?" "It is safe to start my yoga classes again after my neck pain improves?"
Do Some Good and Grow Your Business with Cause Marketing
Cause marketing is truly one of the best ways that you can promote your services as a acupuncture professional. Cause marketing refers to a type of marketing where a business partners with a non-profit organization to help bring awareness to a charitable cause.
Improving Communication Between AOM and Biomedical Providers
How comfortable do you feel talking to Western medical providers? If you are like me, you may not feel as comfortable as you would like. Some of my interactions with MD's haven't been the fruitful steps toward integrative medicine for which I had hoped.
April, 2014, Vol. 14, Issue 04
Techniques to Help Your Pediatric Clients
By Beth-ellen Zang, LMT, AHE, CNC
Used properly, cups can facilitate the body's ability to stay healthy and return itself to a balanced state when things do go awry. In working with infants, toddlers and weak or frail children, it's important to use the small face cups.After two years of age, you can begin using the larger, stronger vacuum cups carefully. Cupping addresses issues like colic, mastoiditis and ear infections, teething, asthma and lung conditions, magnets for bumps bruises and pain, easing soft tissue through growth spurts, muscle spasms (charley horse), scoliosis, indigestion, headache, fevers, sprains, swellings, nervous system sedation, excess excitation like crying and irritability, trouble falling asleep, ADHD and so much more.
First of all, no matter what issue is being presented, the first thing to do is evaluate the situation.
Less is better when treating anyone young or old. Be careful not to over treat. If an infant is having problems with colic, slow and gentle cupping techniques could help calm the nerves and help to draw fluid into the digestive system. Many infants have trouble calming down and cry for hours. Cups can facilitate the calming of the nervous system and help them relax.
In the case of ear infections and mastoiditis, teething and other head related issues, keeping the lymph flowing and muscle tissue soft can help relieve related pain and distress. With lymph flowing and a relaxed system, the body can concentrate on correcting itself and often the problem does not exacerbate or can run its course quickly.
Small cups with magnets attached can help relieve pain from bumps and bruises. They may help keep blood and lymph flowing so the area can heal more quickly.
During growth spurts, children often experience muscle spasms. Using specific techniques with the cups to sedate the central nervous system, softening the muscle tissue can help alleviate a lot of suffering for some children.
Lung issues can be greatly helped either by stimulating stagnancy or clearing congestion, helping to drain excess liquid from lungs or draw water towards them. It is very important to evaluate and apply appropriately.
Using cups to sedate the central nervous system can be invaluable if you have a child with ADD or ADHD.
Keeping the fluids in balance, the central nervous system calm and muscle tissues relaxed assists in the continued health of the body and contributes to the correction of body distress when it is out of balance.
I first started using the cups with my grandchildren. My grandson had pneumonia. I arrived at their house and he was extremely uncomfortable and could not breathe without pain. I spent about half an hour gently treating his whole body to relax him and then concentrating on his lung area as he relaxed more. He got up feeling great and breathing without any problems whatsoever. He continued to feel better and never relapsed. He went back to school two days later with a clean bill of health from his doctor.
Recently, an injured two-year old was brought into my office. She had had a pretty terrible fall on her face and had whiplash. She had a constant headache and backache. It took about fifteen minutes of gentle cupping technique mostly on the spine area, neck and some on her face around the nasal bones and mandible areas. One session had her back to her old self again.
Beth-ellen Zang is the Founding Director of Sedona College of Natural Health. She has had a private medical massage practice and has been a Lifestyle Awareness Facilitator since 1979. In 2005, she was certified as an Ayurvedic Health educator and is still studying Ayurveda with the Kerela Academy. Beth-ellen is a Certified Nutrtitional Consultant and has been a Certified ACE Massage Cupping Educator since 2006.
She can be reached at
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text: We were blown away by the hotel, the staff, and even the view from the windows. We confused our reservations and showed up a day early. The desk staff were exceptionally helpful in accommodating us and adjusting our reservations. The views from all the rooms are great - beautiful mountains on both sides. Our king room and bathroom were large and very nice and well maintained. The beds were very comfortable and the room came with a refrigerator. The breakfast was good but unfortunately in too small an area for such a large group of people. The breakfast staff were overwhelmed - too many people and too small a space, yet they were very efficient, always having a smile and nice disposition, and they did an excellent job considering what they had to deal with. The only downside at the hotel was there was only one elevator which became very busy during the mornings and waits were a few minutes at times. Overall, I would definitely pick this hotel again as a base for our several day adventure in the Banff/Lake Louise/Jasper area. It is much less expensive than hotel near Banff and Lake Louise but provided excellent accommodations.


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