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While in Kuching, take the bus to Kubah on a Sunday morning. The Sunday market will ravish you with its colours, fruits, eateries and as a bonus, the buskers playing all...
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This is was a great news, because you can purchase some fresh food from the Sunday Market then bring all that's to this place call Medan Niaga Satok cooking service. This stall was very helpful and also giving us some idea style cook too. So next time when you guys come to here ,you can easy find them at stall...
While in Kuching, take the bus to Kubah on a Sunday morning. The Sunday market will ravish you with its colours, fruits, eateries and as a bonus, the buskers playing all afternoon! Sit back in the open-air cafeteria with a teh tarik and listen to the band - passers-by join them to sing their favorite song, live karaoke!
Down the road from its previous location the market offers all kinds of local products. Fruits, fish, chickens, souvenirs, pets, plants and lots more. Kinda fun to just wander around. Many good photo opportunities.
The market starts on Saturday until Sunday evening. Dissapointing visit - understand it has recently been housed in this new modern location so has lost its natural etnic charm. Went there on Saturday late afteroon - not all the shops in the food center were open so even the food expereince was not great.
It was fun to look around as the local fresh market. It comes with smells from the seafood and the fresh produce looked good. There are also a few stands for tourists to pick up souvenirs. It's worth a look around.
From the heart of Kuching City i took a taxi to this market. RM20 for a 10 minutes. I went to the market after reading views that i can find local dishes such as Laksa Sarawak and Kolok Noodle here as well as other traditional local food.
Unfortunately i found Laksa Sarawak, dabai, Terung Sarawak, and a local cake, Celurut...
As it has moved to another place nearby, we wasted some time looking for it and ended up late. We went there on Sunday afternoon. Stores are open from Saturday 9am to Sunday 3pm. The stores were closing one by one. We managed to get things that we want to bring back as souvenir. Ikan Terubuk Masin, Telur Ikan Terubuk,...
Well this place is generally a flea market, wet market and dry market. Everything that you need is found here. it is also a good place if you plan to get some gifts for home. T-shirts and accessories is abundent it is possible to haggle your way through the prices. Some of these people will not budge but then just...
The Satok Weekend Market is located just across the Tun Abdul Rahman Bridge at Medan Niaga Satok. Lots of car parking space available. Great place for shopping for anything under the sun. Plenty of stalls selling souvenirs, local snacks & delights, fruits & vegetables, fresh fish & meats, etc.
Great place for getting good bargains. Enjoyed the visit and bought...
...and I wasn't disappointed. There were plenty of stalls that sells local snacks and delights, all flavourful and yummy.
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Marker-assisted pyramiding of leaf rust resistance genes Lr24 and Lr28 in wheat (Triticum aestivum)
Indian Journal of Agricultural Sciences (Impact Factor: 0.14). 01/2011; 81(3):214-218.
Full-textDOI: · Available from: Sudhir Kumar Gupta, Sep 27, 2015
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Article: Marker-assisted pyramiding of leaf rust resistance genes Lr24 and Lr28 in wheat (Triticum aestivum)
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Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.
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Article: Effect of the leaf rust resistance gene Lr28 on grain yield and bread‐making quality of wheat[Show abstract] [Hide abstract]
ABSTRACT: Near-isogenic lines carrying the Lr28 gene developed in five genetic backgrounds were tested for 2 years with and without fungicide treatment. The Lr28 gene increased grain yield, 1000-grain weight and number of effective tillers per plant under heavy leaf rust infection with no negative effects on yield and bread-making quality in rust-free plots. Although a reduction in dough development time was found to be associated with Lr28, it can still be used extensively in wheat breeding programmes.Plant Breeding 06/2008; 123(1):35 - 38. DOI:10.1046/j.1439-0523.2003.00937.x · 1.60 Impact Factor
Article: A diagrammatic scale for estimating rust intensity on leaves and stems of cereals. Can J Res Sect C[Show abstract] [Hide abstract]
ABSTRACT: The Cobb scale and the modified Cobb (U.S.D.A.) scale were devised for estimating, by means of diagrams, the proportion of the area of a leaf or stem occupied by rust pustules. Existing diagrams illustrating these scales are inadequate in being arranged in intervals that are too large and irregular, and in not depicting a sufficient range of pustule sizes. New diagrams are presented using smaller regular intervals of rust intensity and a greater range of pustule sizes, thus providing a basis for more objective and accurate determinations.
- Integrating marker-assisted background analysis with foreground selection for identification of superior bacterial blight resistant recombinants in Basmati rice Marker-assisted wheat breeding: present status and future possibilities. 131-9..


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Jay Paterno encourages participants during the Penn State... News PhotoArts Culture and Entertainment,Dancing,Education,Encouragement,Horizontal,Human Interest,IFC Films,Learning,Marathon,Participant,Pennsylvania State University,Social Issues,USA,University ParkPhotographer Centre Daily TimesCollection: Tribune News Service 2013 MCTJay Paterno encourages participants during the Penn State IFC/Panhellenic Dance Marathon at the Bryce Events Center in University Park, Pennsylvania, on Saturday, February 16, 2013. Dancers started the 46-hour fundraiser on Friday. The event, known as Thon, raises money to help families that are battling pediatric cancer. Last year's event raised $10 million. (Abby Drey/Centre Daily Times/MCT via Getty Images)


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about Todd FlowerdayA Roman Catholic lay person, married (since 1996), with one adopted child (since 2001). I serve a parish in music ministry.
about John Donaghy
John is a lay missionary since 2007 with a parish in western Honduras. Before that he served in campus ministry and social justice ministry in Iowa. His ministry blog is http://hermanojuancito.blogspot.com
He also blogs reflections on the lectionary and saints/heroes/events of the date at http://walktheway.wordpress.com
He'll be a long-term contributor here analyzing the Latin American bishops' document from their 2007 Aparecida Conference.
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Daily Archives: 3 August 2008
The Armchair Liturgist: Marian Music
Here's a question for the musically-inclined. How broad should a parish repertoire be on Marian music? Most everybody has enough hymns and songs to fill at least one Mass. Should there be more? What I mean is this: should the … Continue reading
Posted in The Armchair Liturgist 10 Comments
FIYH 97-100: Revising
The second of four working stages of homily preparation, revising–it's a vital one. [97] The revising stage is one of the most important and the one that is too easily omitted for lack of time. To revise is frequently to … Continue reading
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text: If you have invested in a website but it is not getting visitors or helping to increase sales of your book then reading this blog could help you see where you are going wrong. Even if you have invested in the services of a skilled web designer, it is never enough to just set up an attractive-looking website. Quality and relevant content is vital. Continue reading


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"I think it would be a little disappointing, but hopefully if you're young enough you can go to the next one."
Related quotes
"We're a bit disappointed. We've got a young side here, there's so much to give. Hopefully in the next few games we can put some things together and win some games."
"We never gave up and I give them credit for that. We're young and hopefully we'll be better next year."
"I suffer whenever I see that common sight of a parent or senior imposing his opinion and way of thinking and being on a young soul to which they are totally unfit. Cannot we let people be themselves, and enjoy life in their own way? You are trying to make that man another you. One's enough."
"We're still a young team. Hopefully, this carries over to next year if we get the same group out and even more kids."
"They say with age comes wisdom but the wisdom that you acquire as an old man is really only worth having as a young man."


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Jenni Rivera
Esmeralda Gonzalez and her girlfriend Karina Vasquez from Van Nuys,... News PhotoArts Culture and Entertainment,Burbank,California,Candle,Fame,Flower,Girlfriend,Horizontal,Jenni Rivera,Mexican Ethnicity,Music,Sadness,Singing,Temporary Memorial,USA,Van NuysPhotographer ROBYN BECKCollection: AFP 2012 AFPEsmeralda Gonzalez (L) and her girlfriend Karina Vasquez from Van Nuys, California grieve at a memorial of flowers, candles, ballons and notes to the late Mexican-American singing superstar Jenni Rivera, December 10, 2012 in Burbank, California. Rescue workers searched Monday the wreckage of a plane that carried Rivera, a star on both sides of the border, whose death shocked Latin music fans and celebrities. AFP PHOTO/ROBYN BECK (Photo credit should read ROBYN BECK/AFP/Getty Images)


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SACRAMENTO (AP) - A California judge has rejected a challenge by a farmers' group to agricultural water cuts sought by state regulators during the drought.
YOSEMITE NATIONAL PARK (AP) - World-famous wingsuit flier Dean Potter had strapped his iPhone to the back of his head and hit record before jumping from a cliff in Yosemite National Park in what was to be an exhilarating flight through a V-shaped rocky formation - a route that left little margin for error.
MIDDLETOWN (AP) - A predictable but painful summertime ritual played out in half a dozen resort communities near California's largest freshwater lake on Tuesday as an erratic, week-old wildfire that has wiped out dozens of buildings continued to threaten nearly 7,000 more.
SACRAMENTO (AP) - California restored voting rights Tuesday to tens of thousands of criminals serving sentences under community supervision, reversing a decision by a state official that they could not participate in elections.
STOCKTON - Stockton Animal Shelter is staging a three-day "Empty the Shelter" adopt-a-thon.
MODESTO – The Modesto Junior College Astronomy Club's next Telescope Night is Thursday, Aug. 6, from 9 to 11 p.m. on the fourth floor rooftop of the Science Community Center on West Campus, 2201 Blue Gum Avenue.
POMONA (AP) - Two women died from suspected drug overdoses at a popular weekend music festival at the Los Angeles County fairgrounds, prompting calls for an investigation and concern over the use of drugs at youth-oriented events.
SACRAMENTO (AP) - A union-backed effort to raise California's minimum wage has been approved to start collecting signatures for a statewide ballot initiative.
LOS ANGELES (AP) - Authorities on Monday searched a rugged, five-square-mile area east of Bakersfield, California, as part of a six-day manhunt involving a killing, kidnapping and the wounding of two deputies.
Manteca Firefighters Local #1874 IAFF is again hosting their annual Tip a Fireman event on Wednesday, Aug. 26, from 5 until 9 p.m. in support of the Give Every Child a Chance's free after school mentor/tutoring programs.
SAN FRANCISCO (AP) - A federal judge in San Francisco extended a restraining order on Monday that blocks an anti-abortion group from releasing any recordings that it secretly gathered at annual meetings of an abortion providers' association.
LOS ANGELES (AP) - When water gets scarce and the government slaps restrictions on its use, who should be first in line at the spigot? Farmers, according to an Associated Press-GfK poll.
SAN DIEGO (AP) - A group of San Diego residents is studying the feasibility of adding a barrier to the city's iconic Coronado Bridge to prevent people from jumping to their deaths.
RICHMOND, Ind. (AP) - Police say an eastern Indiana man tried to return to sleep on top of railroad tracks after a conductor woke him to say he had been struck by a train.
NEW YORK (AP) - President Barack Obama will impose even steeper cuts on greenhouse gas emissions from U.S. power plants than previously expected, senior administration officials said Sunday, in what the president called the most significant step the U.S. has ever taken to fight global warming.
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TRUCKEE (AP) - Scientists in the Sierra Nevada have started tracking a mule deer herd north of Lake Tahoe with radio collars to try to learn ...
SAN FRANCISCO (AP) - A San Francisco resident became the first woman to swim the 30-mile stretch from the Farallon Islands to the Golden Gate Bridge ...
LOS ANGELES (AP) - The Metropolitan Water District of Southern California shelled out nearly $57 million during a popular program that paid homeowners and businesses to ...
SAN JOSE (AP) - The river that runs through America's 10th-largest city has dried up, shriveling a source of civic pride that had welcomed back ...
RENO, Nev. (AP) - The U.S. Forest Service has abandoned logging plans it suspended in the mountains above Lake Tahoe nearly two years ago after ...
SACRAMENTO (AP) - Miriam Uribe enrolled in California's low-income health insurance program last November, and she still hasn't found a primary care doctor 10 ...
SAN FRANCISCO (AP) - San Francisco Examiner photographer Mike Koozmin was on a routine assignment at the city's Hall of Justice in the middle of ...
LOS ANGELES (AP) - It began with a routine traffic stop, blossomed into a protest with the help of a rumor and escalated into the deadliest ...
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Check out Birmingham Home and Garden's July/August issue to find out how to make your kitchen remodel successful! Click here to read the article.
Before and After: Family Adventure
Taking on a remodel? Read Twin Tips in Birmingham Home & Garden to learn more
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Inhibition of epithelial cell apoptosis by Porphyromonas gingivalis
Porphyromonas gingivalis is periodontal pathogen that is capable of invading gingival epithelial cells (GECs). Apoptotic responses of primary cultures of GECs to P. gingivalis were investigated with a DNA fragmentation ELISA assay. P. gingivalis induced a transient increase in GEC DNA fragmentation; however, after prolonged incubation GECs did not undergo apoptosis. Furthermore, P. gingivalis blocked apoptosis in GECs following stimulation with camptothecin. Immunoblotting of GECs with Bcl-2 or Bax antibodies showed that P. gingivalis up-regulated Bcl-2 levels in GECs, whereas Bax levels were transiently elevated and declined after 24 h stimulation. Streptococcus gordonii did not affect levels of either molecule. RT-PCR demonstrated that induction of Bcl-2 occurs at the transcriptional level. The results suggest that P. gingivalis can inhibit apoptosis in GECs by up-regulation of the anti-apoptotic molecule Bcl-2. The prevention of host cell apoptosis may represent a strategy for P. gingivalis survival within invaded GECs.
- Invasion
- Apoptosis
- Bcl-2
- Bax
- Porphyromonas gingivalis
1 Introduction
Porphyromonas gingivalis, a Gram-negative anaerobe, is an aggressive pathogen in adult and severe periodontal diseases that are characterized by chronic inflammation of the gingiva, destruction of periodontal tissues and loss of the alveolar bone [1]. P. gingivalis elaborates a vast array of virulence factors including proteinases that can degrade host immune effectors and tissue components, along with toxic metabolites and cellular constituents [2]. In addition to these overtly destructive factors, P. gingivalis engages in an intricate interaction with the epithelial cells that line the gingival crevice. Association between P. gingivalis and these gingival epithelial cells (GECs) results in epithelial cell cytoskeletal rearrangements and calcium ion fluxes that culminate in bacterial internalization within the GECs [2,3]. P. gingivalis cells rapidly accumulate and replicate in the cytoplasm, predominantly in the perinuclear area [4].
Intracellular invasion is a common feature of bacteria that initiate infections at mucosal membranes [5]. In many cases intracellular bacteria can induce or inhibit host cell apoptosis. For example, Salmonella and Shigella can stimulate apoptosis in macrophages, and Legionella can induce apoptosis in both macrophages and epithelial cells [6,7]. In contrast, chronic Helicobacter pylori infection induces an apoptosis-resistant phenotype in gastric epithelial cells [8]. These organisms can impinge upon a number of components of the tightly regulated, multistep intracellular apoptotic pathway, including caspases, nuclear factor κB and the Bcl-2 family of proteins [6⇓8]. As apoptosis allows removal of unwanted or damaged cells, epithelial cell apoptosis can be viewed as a means by which the host eliminates infected cells, thus promoting normal epithelial cell growth and integrity [7]. Disruption of apoptotic control pathways, therefore, has an important bearing on pathogen–host interactions.
The outcome of the interaction between P. gingivalis and host cells with regard to apoptosis is uncertain. P. gingivalis products such as butyric acid and proteases can induce apoptosis in T- and B-cells and in fibroblasts, respectively [9,10]. However, P. gingivalis does not induce apoptosis in peripheral blood mononuclear cells [11] and P. gingivalis lipopolysaccharide may inhibit apoptosis in polymorphonuclear leukocytes [12]. Moreover, there are several reports of epithelial cells surviving for up to 8 days after exposure to P. gingivalis [4,13⇓15]. In this study, we investigated the influence of P. gingivalis on apoptosis in primary cultures of GECs.
2 Materials and methods
2.1 Bacteria and culture conditions
P. gingivalis 33277 cells were cultured in trypticase soy broth, supplemented with yeast extract (1 mg ml−1), hemin (5 μg ml−1) and menadione (1 μg ml−1), under anaerobic conditions at 37°C overnight. Streptococcus gordonii DL1 cells were grown in trypticase peptone broth supplemented with yeast extract (5 mg ml−1) and 0.5% glucose at 37°C under static conditions. Bacteria were harvested and washed in phosphate-buffered saline by centrifugation prior to assay. Bacterial numbers were determined in a Klett–Summerson photometer.
2.2 Epithelial cell culture
Primary cultures of GECs were generated as described previously [3]. Briefly, healthy gingival tissue was collected from patients undergoing surgery for removal of impacted third molars. Surface epithelium was separated by overnight incubation with 0.4% dispase. Single epithelial cells were recovered by centrifugation following digestion with 0.05% trypsin and 0.53 mM EDTA. Cells were cultured as monolayers in serum-free keratinocyte growth medium (Clonetics) at 37°C in 5% CO2. GECs were used for experimentation at 70–80% confluence and reacted with bacterial cells or other test reagents in growth medium under normal culture conditions.
2.3 Assessment of epithelial cell apoptosis
To detect fragmentation of DNA in apoptotic epithelial cells, histone associated DNA fragments were examined in a cell death detection ELISA kit (Boehringer Mannheim). GEC cytoplasmic extracts were added to wells of ELISA plates coated with monoclonal antibodies against histones. The presence of histone-associated DNA fragments was then detected in a sandwich ELISA using anti-DNA peroxidase with 2,2′-azino-di-[3-ethylbenzthiazoline-sulfonate] substrate. Absorbance was measured at 405 nm and background at 490 nm. As a positive control for apoptosis GECs were incubated with camptothecin (2 μg ml−1) for 4 h.
2.4 Immunoblotting
Epithelial cells were lysed by sonication (30 s) on ice and cell debris removed by centrifugation (5000×g 10 min). Protein was determined by the Bio-Rad protein assay. Proteins (2 μg) were separated by 10% SDS–PAGE and transferred to nitrocellulose. After blocking with 0.05% Tween 20–3% bovine serum albumin, blotted proteins were reacted with primary antibodies to Bcl-2 family proteins (Bcl-2, Bax, Bcl-xS, Bcl-xL; Santa Cruz) diluted 1:1000. Antigen–antibody binding was visualized with peroxidase-conjugated secondary antibodies and chemiluminescence detection (Amersham Pharmacia). Duplicate blots were probed with keratin 5/6 antibodies (Chemicon) to ensure equal protein loading.
RNA was prepared from GECs with Totally RNA™ (Ambion). Bcl-2 specific primers were: Bcl2-1, 5′-CACCTGTGGTCCACCTGAC-3′; Bcl2-2, 5′-AGGGCCAAACTGAGCAGAG-3′. Reverse transcription was performed using RETROscript™ (Ambion), following the protocol recommended by the manufacturer. Briefly, 2 μg of RNA was denatured at 85°C for 3 min in 12 μl solution containing 100 pmol of oligo(dT). The enzyme mixture (2 μl of 10×RT buffer, 4 μl of dNTP mix, 1 μl of RNase inhibitor, and 1 μl of reverse transcriptase) was added and incubated at 42°C for 1 h. The enzyme was inactivated by incubation at 92°C for 10 min. The resulting cDNA was amplified in a 20 μl PCR mixture containing 1×PCR buffer, 1 μl of cDNA, 2.5 mM MgCl2, 10 mM dNTP mix, 50 pmol of each primer, and 1 U of Taq DNA polymerase (Promega). The amplification conditions were as follows: denaturation at 94°C for 30 s, annealing at 65°C for 30 s and elongation at 72°C for 30 s, for 30 cycles. As a control, cDNA was also amplified with primers for actin (Act-1, 5′-AGAGCTACGAGCTGCCTGC-3′; Act-2, 5′-AAAGCCATGCCAATCTCATC-3′).
3 Results
3.1 GEC apoptosis
Our previous results [4,13] and laboratory observations show that exposure of GECs to P. gingivalis for up to 48 h, at a multiplicity of infection (MOI) ranging from 10 to 1000, does not affect epithelial cell viability as evidenced by intracellular hydrolysis of calcein-acetomethyl ester and physiologic intracellular calcium ion concentrations. These qualitative viability observations were quantitated with regard to apoptotic cell death by a cell death detection ELISA (Fig. 1). After 2 h stimulation of GECs by P. gingivalis (MOI 100) DNA fragmentation in GECs was initiated. As DNA fragmentation precedes some of the more visible markers of apoptosis such as plasma membrane breakdown, this finding is not inconsistent with results from visual inspection of GECs after 2 h exposure to P. gingivalis that show phenotypically normal cells [4]. After 24 h P. gingivalis GEC incubation, DNA fragmentation levels had returned to normal and the GECs were not apoptotic. In contrast, S. gordonii, a non-pathogenic, non-invasive oral organism, induced apoptosis in GECs. Moreover, a 24-h preincubation with P. gingivalis blocked GEC apoptosis induced by camptothecin. Identical results were obtained at a bacterial MOI of 1000 (not shown). Thus, P. gingivalis appears to actively suppress apoptosis in GECs.
3.2 Levels of expression of proteins associated with apoptosis
Immunoblots of GEC lysates showed no detectable expression of the anti-apoptotic protein Bcl-2 in unstimulated GECs or in cells stimulated with P. gingivalis for 2 h at a MOI of 100 (Fig. 2). In concordance with the cell death assay however, after 24 h incubation P. gingivalis induced expression of both the ∼26- and ∼29-kDa isoforms of Bcl-2. Exposure of GECs to S. gordonii did not result in detectable Bcl-2 expression under any of the test conditions, a result also consistent with the apoptosis assay. Expression of the anti-apoptotic protein Bcl-xL was not affected by P. gingivalis (not shown). Identical results were obtained at a bacterial MOI of 1000 and with cells derived from two different individuals. Heat-killed P. gingivalis did not induce expression of Bcl-2 or Bcl-xL.
As infection by P. gingivalis induced DNA fragmentation at the initial stage, expression of the pro-apoptotic proteins Bax and Bcl-xS was investigated. Resting and S. gordonii-stimulated cells did not exhibit detectable Bax expression (Fig. 3). However, P. gingivalis induced Bax expression in GECs after 2 h exposure. After 24 h exposure Bax returned to undetectable levels. Induction of Bax by P. gingivalis required viable cells as heat-killed P. gingivalis did not affect Bax levels. The finding that S. gordonii did not cause an increase in Bax expression suggests that cell death in response to this organism is Bax-independent. Expression of Bcl-xS by GECs was not altered by P. gingivalis infection (not shown). Identical results were obtained with cells derived from two different individuals and with a bacterial MOI of 1000.
3.3 Correlation of Bcl-2 protein and mRNA levels
To investigate if the observed elevation of Bcl-2 expression is associated with an increase in gene transcription, steady-state levels of mRNA were determined by RT-PCR (Fig. 4). P. gingivalis caused a significant increase in Bcl-2 mRNA levels compared to normal or S. gordonii-stimulated GECs.
4 Discussion
Apoptosis, or programmed cell death, is a fundamentally important biological process that is required by multicellular organisms to eliminate redundant, damaged or infected cells and so maintain normal development, integrity and homeostasis of the organism [16]. Apoptosis is characterized by cell shrinkage and condensation of the nucleus along with specific protein degradation and DNA fragmentation. There are at least six surface receptors on mammalian cells that transduce apoptotic signals subsequent to ligand binding [6]. Intracellularly, the major components of the apoptotic machinery include a family of Bcl-2 proteins [17] that integrate diverse upstream signals to control the cellular commitment to apoptosis, and a family of cysteine proteases (caspases) that act downstream of Bcl-2 and cleave critical target proteins to effect cell death [16,17].
Increasingly, it is becoming appreciated that epithelial cell responses to pathogenic bacteria can involve either elevated apoptosis, as in the case of Salmonella dublin and Legionella, or suppression of cell death, as in the case of H. pylori. Most commonly, bacterial modulation of epithelial cell apoptosis has been studied in cell lines [7,8]. Such models have obvious limitations inasmuch as these cells are likely to have limited control over apoptotic pathways. We have developed a culture system to maintain primary GECs in vitro for a limited number of passages (typically five to seven) [3,4]. These primary, short-lived, epithelial cells were utilized to study the apoptotic responses to the periodontal pathogen P. gingivalis. Expression of Bcl-2 family members in unstimulated GECs was low, not detectable by immunoblotting with commercially available antibodies. This contrasts with gastric epithelial cell lines in which Bcl-2, Bax, Bak and Bcl-xL are expressed [8] and may be the result of either tissue-specific differences in expression, or tighter control of Bcl-2 family proteins in non-transformed cells.
As measured by a DNA fragmentation ELISA, the early response of GECs to P. gingivalis infection was induction of apoptosis. However, after 24 h of exposure to P. gingivalis, DNA fragmentation levels had returned to background and the cells were not apoptotic. P. gingivalis cells invade GECs rapidly and in high numbers [3], and antibiotic protection assays indicate that individual GECs can harbor several hundred P. gingivalis, results that are corroborated by direct microscopic observation [4]. Thus, despite the burden of large numbers of intracellular P. gingivalis, GECs do not undergo programmed cell death over at least a 24-h timeframe. Moreover, exposure to P. gingivalis protected GECs from apoptosis induced by camptothecin, a human topoisomerase I inhibitor used as a chemotherapeutic agent in cancer treatment [18]. Similarly, H. pylori, which is associated with gastric cancer, confers resistance to apoptosis through decreased expression of p27kip1[8]. These findings suggest that P. gingivalis may disrupt tissue homeostasis in the periodontal pocket and allow the intriguing speculation that P. gingivalis could be associated with some forms of oral cancer.
GEC apoptotic responses to P. gingivalis were correlated with expression levels of certain Bcl-2 family proteins. Bcl-2-like proteins can be functionally categorized on the basis of their ability to either promote (e.g. Bax, Bcl-xS, Bad) or suppress (e.g. Bcl-2, Bag, Bcl-w and Bcl-xL) apoptosis. Apoptosis may be controlled by interactions with the scaffold proteins necessary for the assembly of certain caspase precursors, or by the formation of ion channels in mitochondrial membranes that affect permeability and release of cytochrome c, thereby also modulating caspase activation [17]. Increased expression of Bcl-2 by P. gingivalis is, therefore, likely to make a major contribution to apoptosis resistance. Moreover, as one outcome of camptothecin treatment is reduced Bcl-2 expression [18], induction of Bcl-2 by P. gingivalis could also account for the ability of the organism to block the activity of camptothecin. While levels of Bcl-2 were elevated, Bcl-xL expression was not modulated by P. gingivalis. This is consistent with earlier studies that have shown that Bcl-xL has a pattern of expression that is distinct from Bcl-2 [19]. In contrast to Bcl-2, expression levels of Bax and Bcl-xS in GECs were not above normal resting levels after 24 h exposure to P. gingivalis. As pro- and anti-apoptotic Bcl-2 family members can heterodimerize and titrate one another's activity [17], low levels of pro-apoptotic proteins will not interfere with the ability of Bcl-2 to function in an anti-apoptotic capacity. Interestingly, levels of Bax were transiently induced by P. gingivalis, increasing after 2 h bacterial exposure before returning to control levels after 24 h. This finding, coupled with the transient increase in DNA fragmentation, suggests that the first response of GECs to P. gingivalis infection is to initiate apoptotic pathways but these are then stalled by P. gingivalis through up-regulation of Bcl-2 and down-regulation of Bax.
The mechanisms by which P. gingivalis impinges on control of Bcl-2 family members remain to be characterized at the molecular level. Heat-treated P. gingivalis were unable to induce Bcl-2. This may be the result of failure of heat-killed P. gingivalis cells to locate intracellularly [4], or the denaturation of a protein necessary for induction of activity. In either event, P. gingivalis is an active participant in the process.
In conclusion, we have established that P. gingivalis does not induce apoptosis in primary cultures of short-term gingival epithelial cells, and furthermore, that P. gingivalis-infected cells are resistant to apoptosis induced by camptothecin. Apoptosis is associated with elevated levels of Bcl-2 and suppression of initial elevation of Bax. The ability to suppress apoptosis may be an important survival strategy of P. gingivalis, facilitating retention within the periodontal tissues. Further definition of the molecular basis of apoptosis may provide insights into the pathogenicity of P. gingivalis and of the full range of diseases related to this invasive organism.
Supported by NIDCR DE11111 and DE07023.
- © 2001 Federation of European Microbiological Societies


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2008 Toyota Tacoma Problems
The amber front running lights, located in the head light assembly may melt or crack their lens. The correction at this time is to replace the head light assembly.
At higher mileages, (125,000-150,000) the automatic transmission may not shift correctly. This can be caused by the throttle position sensor being out of adjustment or a shift solenoid needing to be replaced. Typically the transmission does not need to be completely overhauled.
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when vehicle is changing gear it seems i get a heavy vibration (up and down) for a few seconds. most time it seem as if this happens where the road is a little wavy
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Construction valuation of residential complex on 2-acre lot tops $17 million
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Matching up the Bruins-Rangers series - 0 Comments
Boston and New York are natural geographical rivals, and that has always extended to the cities' sports teams. There are the Red Sox and Yankees, of course. The Celtics and Knicks have had their battles through the decades. And the Patriots have been fighting a two-front war against the Jets and Giants.


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The Stage Deranged in the Dirt
AMSTERDAM - The show opens without the usual curtain. A square platform on the stage is covered with loose, dark earth; at the front, a metal coffin on two wooden planks over a hole that has been dug. A metal frame hangs in the background, around a screen made of gold thread. Beyond that, one can see a lit floor, a banquet table and six figures in black, walking back and forth. One holds something resembling a torch.
The lights in the theater in Amsterdam are off, and an image of a man is projected onto the gold-threaded screen. To be more exact - a mane of blond hair, a forehead, eyes and a nose. The picture is poor in quality, as if the camera was positioned very close to his face. The face begins to recite the famous "To be or not to be" monologue, in a new translation into German by Marius von Mayenburg (a playwright and dramaturge whose work "The Ugly One" has been performed at the Haifa Theater). We don't see the mouth moving with the text. The actors in the background take down the picture, hinting at the approach taken here: This is the story as Hamlet sees it in his head.
When Thomas Ostermeier became artistic director of Berlin's Schaubuehne Am Lehniner Platz theater in 1999, one of his aims was to bring classic drama to the German audience. He could not have selected a more well-known and admired work than William Shakespeare's "Hamlet." He himself directed the production, which premiered last year at the Festival d'Avignon, and is now touring Europe. I caught a performance in the new hall of the Amsterdam City Theater; this week it will play London.
When a theater director takes on a classic, especially one familiar to those who have never read or even seen it, the challenge is not only to say something new, but to be careful not to fall into the same old traps as well. Everyone knows the play contains the father of all monologues - "To be or not to be" - the question of all questions. I don't know if Ostermeier recalls how in Laurence Olivier's performance of Hamlet, shot more than 60 years ago and one of the most successful screen portrayals of Shakespeare, he is shown seated near the sea, blond and contemplative, while in a voice-over we hear him speaking the lines as if he were thinking them.
Gertrude the movie star
In Ostermeier's production, the face of Hamlet is Lars Eidinger, the only one of the six actors to play just one part. He is tall and ungainly (wearing padded clothing to appear heavier than he is), far from the typical image of the brooding and moody prince. His speech is hesitant rather than fluent, as if he is chewing the words.
The monologue ends and the show begins with the funeral of the King of Denmark, Hamlet's father; without words, with music building up to a climax. Claudius and the queen, in a white wedding dress, approach the coffin. A moving video camera accompanies them, like a paparazzo chasing celebrities. Gertrude, in a blond wig and sunglasses, is a movie star.
Claudius, brother of the late king, with a balding head and in evening attire, the faithful companion, holds one of her arms. One actor offers them black umbrellas. Another picks up a water pipe from the floor and sprays them with rain. A grotesque gravedigger tries to place the coffin in the hole, in what turns into a slapstick scene. He puts the coffin in upside down, jumps in the hole to turn it around, begins to cover it with dirt, remembers that the members of the burial party should throw in the first clumps of earth, leaps in the hole again to remove the dirt, runs around and finally hands the shovel over to the queen. In the end the coffin is covered and a mound of earth remains on the front of the stage, as if waiting for a grave stone.
At the end of the burial, the stage floor with the banquet table moves forward for the wedding feast. The queen (Judith Rosmair), in her white bridal gown and veil, takes a microphone and sings a seductive love song to her new husband. Before Claudius rises to speak, one of the actors silences the crowd with a volley of gun shots into the air. Claudius parts from Laertes, who requests permission to study in France. Polonius and Horatio eat in silence, and the video camera reveals that they are actually eating dirt. Claudius asks Hamlet to stop grieving for his father and then hugs him; when he lets him go, Hamlet collapses on the mound over his father's grave, his face in the mud. We see only his back and blond hair. When he rises, his face is covered with brown dirt and he holds a lump of earth in his hand.
Ostermeier's production speaks in sharp visual images. The earth is the subject, as well as where the action takes place. Examining the text, one discovers that this is a beloved word for Hamlet (as is the image of a mole digging in the earth, the same earth where the body is eventually buried).
While the gravedigger holding Yorick's skull has been cut from this version, the production takes place entirely in a graveyard, at the edge of a grave. In the scenes that follow, Hamlet and the character of Ophelia (played by Rosmair again, minus the blond wig, in pants and a white shirt, and later in a black dress) wallow in the brown earth, as turns sharply from the tenderness of love and lovemaking toward brutality and abuse.
The audience quickly understands that this Hamlet is not pretending to be insane; he in fact went mad a long time ago. A comedic clown who paints his face and runs around the stage, he shoots at the screen behind which Polonius is hiding and Polonius falls to his death, hanging from gold threads.
If it weren't for the scene in which Claudius confesses his crimes (lines which cannot be ignored; they are written in the play), it might be possible to think Hamlet concocted the whole story, and is forcing the Danish court to serve his madness. Perhaps both things are happening: Denmark is a prison where something is rotten, and the chief prisoner is a madman who intensifies the corruptness of life to produce a farce that ends with corpses.
Though Yorick's skull does not appear in this production, it is shown over and over on video, merging with the image of Hamlet like a kind of grotesque crown on his head. When the cameras do not show frenetic close-ups, we see black rips in the screen, as if remnants of Hamlet's noble spirit were disintegrating in front of our eyes.
Embracing slapstick
The two-and-a-half hour show is an attack on the senses, in terms of both plot and dialogue, and in the movement of the lit-stage platform, the wedding banquet table where Hamlet encounters his mother and the screen behind which Polonius hides. The platform and the screen move back and forth while we spectators keep our eyes on the screen, what is projected on it and the figures behind it.
The show does not disdain wild slapstick comedy, as seen in the play-within-the-play mounted by Hamlet and Horatio, in which Hamlet plays the queen naked from the waist up, in net stockings and high heels; this portion of the play is not faithful to the text, meter or rhyme. And when Hamlet learns he is being sent away to London, he fantasizes about Picadilly Square and Big Ben. But the duel at the end is portrayed with life-and-death seriousness. Eidinger and Stefan Stern (who plays Laertes and Rosencrantz; Robert Beyer portrays Polonius and Osrick; Urs Jucker takes on Claudius and the ghost of the father - perhaps the only acceptable doubling of roles in traditional performances) run after each other on the brown earth and land blows over and over again.
Hamlet wins out over Laertes in the end, but is wounded by the poisoned sword. He has just seen the queen swallow from the poisoned cup (a few minutes earlier we saw the same actress playing Ophelia, lying on a sheet of plastic held by two actors behind the screen, and pounding it, as the video cameras projected an image of her drowning), and sticks the sword into Claudius, who manages to raise a glass of wine in mockery before Hamlet forces him to drink from the poisoned cup as well.
Hamlet lurches toward the front of the stage with the last of his strength, the gold-threaded screen following behind; he is separated from the audience by the screen's final image: the limbs of corpses. In the background we hear Fortinbras declaring the rights to this piece of land (the earth again). Hamlet, at the edge of the stage, takes a long look at the audience and says, "The rest is silence."
I am not sure whether this is the most brilliant production of "Hamlet" I've seen; it is certainly not a complete interpretation, despite the attempt to crack open something new within the play. It was, however, an intense experience - an encounter with Hamlet's distorted world via theater that does not try to create an illusion of reality. On the contrary, this is theater that constantly reveals to the audience the means by which it is achieving its effects. Paradoxically, in this way, this production allows us to reach Hamlet's extreme situation - as he both fights with reality and is swallowed up by it, trying to survive and not dissolve, and in the end exterminating it along with himself. This is "Hamlet" in the extreme, after which there is no choice but to go back and read it again, in a blazing light, and recall the torn figures on stage that night.
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Fergus Falls (FFHS) Class of 1978 Alumni List
Help us Update the Fergus Falls 1978 Class List by adding any names missing from the list below:
1978 Class List
- John Bigwood
- Mary Bruening
- Mark Christopherson
- Debra Denbrook
- Mike Eveland
- Allen Fyhrie
- Marty Gadow
- Middleton Joan
- Corey Johnson
- Mark Krogh
- Mitchell Laney
- Jane Marshall
- Joseph Nehk
- Jan Olson
- Becky Ripley
- Kevin Schroeder
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Grease was the top movie in theaters in 1978, with a lifetime gross of over $181,813,770. Among TV shows in 1978, Laverne & Shirley was the most popular of the year.
Hit Songs From 1978
What's Your NameLynyrd Skynyrd
Sentimental LadyBob Welch
Sweet Talking WomanElectric Light Orchestra
Night FeverBee Gees
Still The SameBob Seger and The Silver Bullet Band
Dust In The WindKansas
Don't Look BackBoston
Short PeopleRandy Newman
Can't Smile Without YouBarry Manilow
Three Times A LadyCommodores
Hit Movies From 1978
- Jaws 2
- Animal House
- Hooper
Hit TV Shows From 1978
- Eight is Enough on ABC
- Vega$ on ABC
- The Ropers on ABC
- Monday Night Football on ABC
- Soap on ABC
Fergus Falls High School News
More 1978 alumni from Fergus Falls HS have posted profiles on Classmates.com®. Click here to register for free at Classmates.com® and view other 1978 alumni.
The students that went to school at the Fergus Falls high school called Fergus Falls High School and graduated in '78 are on this page. Register to add your name to the class of 1978 graduates list.
Don't forget to upload your Fergus Falls High School pictures and check on the latest info about any Fergus Falls High School reunion.


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Originally presented in Bilbao, Spain at Nonick 2011 by JESS3 CEO & Founder Jesse Thomas and JESS3 Director of Strategy Brad Cohen.
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As a teenager, filmmaker Pearl Gluck left her Orthodox Jewish clan in Brooklyn for secular life in Manhattan. Many years later, Pearls father has one wish: that she marry and return to the community. Pearl, however, takes a more creative approach to mend the breach. She travels to Hungary to retrieve a turn-of-the-century family heirloom: a couch upon which esteemed rabbis once slept. En route for the ancestral divan, Pearl encounters a colorful cast of characters who provide guidance and inspiration, including a couch exporter, her ex-Communist cousin in Budapest, a pair of Hungarian-American matchmakers and a renegade group of formerly ultra-Orthodox Jews. Nimbly clever and intensely illuminating, DIVAN is a visual parable that offers the possibility of personal reinvention and cultural re-upholstery. This DVD edition features over 30 minutes of additional footage, including deleted scenes, follow-ups and Q&As.
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Interesting insight into the Hasadic community with a good story to boot. Definitely worth watching.Published 9 months ago by Javert
I saw this on TV and found it better on pbsi hpe you are happy.
P. S. I was interesting .
I think that if you're not from the Chassidic world, you might find this film interesting. However, if you are orthodox or know anything about Chassidim, there are no major... Read morePublished on October 10, 2006 by Aaron Huber


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amazing view!!
All the best to You:)
Hello dear Udo!
Very nice picture with so tender colours of the sky. I like this kind photo, in which I can see a simple page of our life. This photo have a good mood. this chopsticks looks a very nice, an another your photo where I saw them on the field, I read that they collect by hands, this is a great and a hard work.
Have a nice day, Irina
Hello JuliaL!
Dear Julia, great thanks for your visit and very kind comment + Like! I'm very happy you like this kind of photo!
This photo is showing all the unfineshed chopsticks from a factory at countryside! Additional you can find chickens between the sticks!:)
Best wishes and a happy weekend for you, Udo
Hello dear Irina!
Great thanks for your visit and very kind comment + Like! I'm very happy you like this photo!
The other photo (Fishing at DaliShan) what you mentioned is showing only the harvested rice field and is different to this photo of chopsticks! But it was really amazing to see so many pieces of chopsticks (the factory was on the right hand of this photo). Of course, all of them are now unfinished and have to be cutted for the right length!:) Did you saw the chickens between the chopsticks??:)
My best wishes and a happy weekend for you, Udo
Yes, dear Udo , at first I don't saw them, now I see chickens between the chopsticks :)))
Have a nice weekend, _Irina-
Hello dear Irina!
Great...and later we can eat the chickens with the chopsticks! Only when i'm hungry i can use the chopsticks very well!:)
I wish you a very nice saturday, Udo
Hi dear Udo!
Okay, but I never ate chicken with chopsticks, and that in China everything is eaten with chopsticks, or will it be pilaf with chicken? Today, I briefly have to go to work, and where we meet?
Smiling, Irina
Hello dearest Irina!
1000 and smilng thx for your wonderful reply!
Yes, here in China all chinese eat the chickens, ducks, etc. with chopsticks! Also me can do it...but often i hold these dishes with my fingers!:) I'm very sorry that you have to work at saturday, but....i think...we will meet us for a dinner!:)
Asways smiling is one of the best...and keep it...in this sence i wish you only the best, Udo
Good evening dear Udo!
Sorry, I just came at home, I think today we'll have not time or not? :)))
Smiling, Irina
Hi dear Irina,
i'm very glad about your happy making words and really happy that your hard day at work is finish!
For a dinner...it's never too late!:)
Happy greetings, Udo
ILIKE tecnology and rural! rafl
Hello Rafl!
Thanks a lot for visit and your kind comment! I'm very happy you like this photo!
Best wishes from China-Germany, Udo
Very good capture.Greetings.
Hello sonjabgd!
Many thx for your visit and kind comment! I'm glad you like this photo!
Best wishes from China-Germany, Udo
Photo taken in Dongzhi, Chizhou, Anhui, China


text:
By Kinevia
Open iTunes to buy and download apps.
MetrO helps you find your way in the public transportation systems in more than 400 cities around the world.
One application for all your trips: download the cities you need and search your route by subway, bus, tram, railway... (depending on cities). NO network connection is required after you have installed the cities.
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Don't forget that MetrO is the result of a collective effort: our data can be kept up to date only with your help!
What's New in Version 1.0.9
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- Category: Travel
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- Languages: English, Basque, Bokmål, Norwegian, Bulgarian, Catalan, Chinese, Croatian, Czech, Danish, Dutch, Estonian, Filipino, Finnish, French, Georgian, German, Greek, Hungarian, Indonesian, Italian, Japanese, Korean, Macedonian, Malay, Polish, Portuguese, Romanian, Russian, Serbian, Simplified Chinese, Slovak, Slovenian, Spanish, Swedish, Thai, Traditional Chinese, Turkish, Vietnamese
- Seller: Kinevia
- © Kinevia
Compatibility: Requires iOS 6.0 or later. Compatible with iPhone, iPad, and iPod touch.


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For years eggs have been portrayed as a food that you should not eat for fear of raising your blood cholesterol levels. Recent studies have proven that this is not the case and in fact eggs have been found to be a nutritional goldmine. One large egg has 210 mgs. of cholesterol which seems like a lot, but a recent study from the University of Connecticut suggests that even after eating three eggs a day for 30 days, the ratio between HDL( good cholesterol) and LDL( bad cholesterol) did not change. The result is no increase or change in one's risk of heart disease.
In another study very significant study of 120,000 men and women conducted in 1999 at Harvard University, they found no correlation between the consumption of eggs and heart disease. Nor did the study find an association between egg consumption and strokes.
There's also evidence that eggs help to create a feeling of fullness due to the high protein content. The Journal of the American College of Nutrition in 2005, conducted a study of overweight women. In the study, those who had two eggs for breakfast felt fuller afterwards and ate fewer calories at lunch than women who had a bagel based breakfast with the same amount of calories resulting in more long term weight loss for the egg eaters.
The unsaturated fats and other nutrients including B vitamins may even be beneficial to heart health. It is the saturated fat-rich foods that are commonly consumed with eggs (bacon, sausage, cheese and biscuits) that can raise blood cholesterol and increase the risk of heart disease. A large egg has only 1.5 grams of saturated fat and about 70 calories, so it is the perfect diet food…..just be sure to watch the side of bacon.
For those of you who commonly toss the vitamin rich egg yolks, take note that they are a rich source of lutein and zeaxanthin, relatives of beta carotene that keep eyes healthy and have been linked to a reduced risk of age related eye disease. A 2006 study in the Journal of Nutrition found that women eating six eggs a week for 12 weeks had increased macular pigment which is thought to protect the retina of the eye from the damaging effects of the light.
Other Eggs facts:


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The DC Center for the LGBT Community will present Rayceen Pendarvis with a distinguished service award at our annual Fall Reception on Friday September 18th......
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OverviewSeamless Entertainment, Inc. is an Austin based development studio that was founded in 2008 as a division of Black Lantern Holdings, Inc.. It was the company's second studio after Black Lantern Studios, Inc.
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TV Series Review
Who knew Elizabethan-era politics were so much like 21st-century high school?
Granted, Mary, Queen of Scots doesn't need to study algebra or practice for the soccer team in CW's Reign. She has no SATs to prepare for, no after-school job to work. No, teen queen Mary had just oodles of time to engage with what high school is often really about: dreamy dudes and gossipy friends. Cue the drama!
Every episode of Reign seems obsessed with who's kissing (or bedding) whom and who's obsessed with what and why can't parents understand that true love is way more important than a dull dynastic claim to the throne. In CW's telling, the whole 16th century was a little like a never-ending prom, complete with dresses and dancing and bland indie music tinkling in the background. All that's really missing are crepe paper streamers hanging on the castle walls.
Mary, by definition, is the queen bee of her own angsty and cliquish cotillion, cooling her heels in France while she waits around for someone to sweep her off her feet. She's the belle of the ball by royal decree, the girl every boy wants to smooch. Sure, she has a steady already—a French prince named Francis whom she's been engaged to since she was 6. Everyone says they make a great couple, and if Mary had a locker, she'd certainly have pictures of Francis taped to the door, surrounded by little hearts.
But true love in teen TV dramas runs neither deep nor smooth. Francis' mother, Catherine, can't stand Mary. Francis' dad, King Henry, wants his son to treat her more like a forward-facing pawn than the love of his life. And, as mentioned, Mary has no lack of suitors trying to convince her she can do better. Mary herself finds her eye wandering to Francis' older, illegitimate (and wholly fictitious) brother, Sebastian. (They call him Bash, because, well, this is a CW show.)
Why, it's enough to drive poor Mary half insane with late-adolescent sexual confusion. If only there were a social network on which she could vent.
Of course no one expects historical authenticity from the CW. The network churns out lusty, teen-centric soaps, not PBS-level docudramas. Still, you know that when a show's idea of Elizabethan dress trends toward strapless eveningwear … when notorious mystic Nostradamus takes on the sheen of a sensitive, dreamy guy who'd sit in the back of English class … when Mary does the tango, a dance that was ever-so-scandalous when it was invented in the 1890s … then you know it has taken some serious liberties.
It's all very silly and, of course, salacious. Mary and her ladies-in-waiting indulge in quite a lot kissing and sighing and moaning (some of it induced by partners, some of it in a solitary manner). Fair maidens exchange double entendres with ruddy men and swap tawdry gossip. We hear loads of talk about illegitimate children and royal affairs.
"This is yet one more example of the way that the entertainment industry is attempting to bring premium cable-level violence and sex to prime-time broadcast TV," writes Christopher Gildemeister, the Parent Television Council's senior writer and editor. "Truly disturbing is the fact that such content is being squarely targeted at the CW's key audience of teen and pre-teen girls—an audience already regularly sexualized and exploited by the CW and the entertainment industry generally."
War is frequent. Stabbings are common. Heads can and do roll. If Reign lives a long life on TV, we could even see the protagonist's own life cut short—unless, of course, the CW rewrites history so much that Mary and Francis live happily ever after. (Which, given the CW's track record, seems more likely than not.)
But methinks Reign will get the ax before Mary does. Because it's not just the conservative PTC that's condemning this careless creation. "At some point—and I think this may be that point—it's time to speak the truth," posits The Hollywood Reporter's Tim Goodman. "The CW is ruining America."
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Readability Age Range
Adelaide Kane as Mary Queen of Scots; Megan Follows as Queen Catherine; Toby Regbo as Prince Francis II; Torrance Coombs as Sebastian; Alan Van Sprang as King Henry II; Jenessa Grant as Aylee; Celina Sinden as Greer; Caitlin Stasey as Kenna; Anna Popplewell as Lola; Rossif Sutherland as Nostradamus


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TripAdvisor Reviews Grand Hotel Catanzaro
Travel Blogs from Catanzaro
A relaxing day at sea
Very pleased today to be at sea. It's been non stop for us for over two weeks now - so really nice to just chill and do nothing much. Which is exactly what we did. Late breakfast, swim in the adults pool at the far end of the boat. The seawater was smooth, calm and glassy and we passed the volcanic island of Stromboli which was steaming away nicely. Looked very ethereal with all the sea mist and the glassy water. Then we sailed ...
Off to Crotone but pushed into Le Castella
... says little wind and calm seas!! We know it lies but not this much! So we will sit it out and see what the weather is like tomorrow. Need to repair the sail bag as lazy jacks snapped during the day when adjusting the reefs and we need to go up the mast to replace them. Not a job whilst on the move. Think we may be on the bottom tonight as checking the depth gauge it is still reading zero but hey it's flat!, Waking up after ...
Paestum to Lorica
Wednesday 13th May
There are three national parks heading south towards the toe of Italy and we can only find one campsite between them all. We took the scenic road through the mountains hoping that we might stumble on one but with no luck. For as far as the eye can see, there are forest clad rolling hills with the occasional village perched on a rocky promontory.
Busy last day
Drove into Perugia today. The underground city is blocked off at the moment so we could not visit it. We walked around for a few hours and we went to another old city called Todi. Went shopping and went home to Pablo's I played with Pablo's microphone and he gave us some drum sticks to play ...
On the Road Again
... and realized Rob was in 29C and I was in 42K on the Rome/Toronto leg of the trip. The extremely rude Alitalia agent blamed our travel agent and rudely dismissed us by rudely saying there was nothing she could do (did I mention she was rude?). So off to Rome we went and half an hour later, after an advertised "9 minute" walk to our Rome/Toronto gate (we were in the passport control line-up longer than that, let alone the marathon walk), ...
Other places to stay in Catanzaro
Hotel GuglielmoVia A.Tedeschi 1, Catanzaro | 4 star hotelfrom $162
StillhotelVia Melito Porto Salvo, 102, Catanzaro | Hotelfrom $85
Bellamena HotelVia Fratelli Plutino, 14, Catanzaro | Hotelfrom $60
Hotel club Cala Longavia dei Marinai 2, Catanzaro | 3 star hotelfrom $98