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SBI Smart by SBICAP Securities Track live Indian financial markets, news, monitor your stocks and trade on the move. SBICAP Securities Limited introduc … 50,000+ downloads, Rating 3.31 (1878) Free
Word Search Brazil 2014 Free by Boriol Game remind all Brazilian players that won the World Cup: - Suécia1958. - Chile 1962. - Mexico 1970. - USA 1994. - Sout … 5,000+ downloads, Rating 3.82 (51) Free
Coffee Maker by ICAW (I Can And Will) Do you have what it takes to make the best cup of Coffee in town! Time to grind and roast those coffee beans to bring ou … 100,000+ downloads, Rating 3.46 (1012) Free
TouchPal Emoji OS Phone Theme by 1 Up Themes Please note that this keyboard theme requires TouchPal Emoji Keyboard installed and enabled on your device. Click here t … 1,000+ downloads, Rating 4.36 (64) Free
Party Game Updated by Legion Of Apes LLC Adventure Party Game - Puzzle Style - Free download and play! - Make new friends, meet new characters and hangout in th … 100+ downloads, Rating 4.47 (17) $1.28
Hearts Love Go Launcher Theme by spikerose Hearts Love Theme for GO Launcher EX, includes more than 200 HD icons 217x217px and 6 HD wallpapers 1440x1280px ►Notice … 10+ downloads, Rating 5.00 (6) $4.99
Tune Me Pro ★ Key by Atonality O━┳┳ The PRO key for Tune Me. Download and open this key to unlock, then download the full app "Tune Me" separately. R … 10,000+ downloads, Rating 3.66 (637) Free
Panorama 360 Updated by TeliportMe Inc. Panorama 360 is the best panorama camera app letting you create 360 degree PRO quality panoramas for FREE instantly. * … 5,000,000+ downloads, Rating 3.92 (57738) Free
ProxyDroid by Max Lv ProxyDroid is an app that can help you to set the proxy (http / socks4 / socks5) on your android devices. Help to trans … 1,000,000+ downloads, Rating 4.09 (16985) Free
Nischint Parental Guidance App Updated by Nischint Technologies •Nischint is a Hindi word which means "carefree" and we want to personify this by impacting the lives across the worl … 10,000+ downloads, Rating 3.64 (585) Free
BusNavi by mobiCORE Public transport timetable, trip planner, map - everything you need to ride public transit. After installing the applica … 1,000,000+ downloads, Rating 4.54 (28517) Free
TouchPal Emoji Pixel Theme by 1 Up Themes Please note that this keyboard theme requires the TouchPal Emoji Keyboard installed and enabled on your device. How do I … 1,000+ downloads, Rating 4.36 (22) Free
It's a Movie! Cinema Quiz by It's Sauquet It's a Movie! - the new generation of mobile quiz games is here! It's a Movie! brings a whole new meaning to the word qu … 5,000+ downloads, Rating 3.93 (46) Free
C Program Example by Sharper C is one of the most popular language among all Features : * No need of internet connect for learn c program * List of … 1,000+ downloads, Rating 4.24 (62) Free
マジャン (漫画) by EVERYSTAR Co., Ltd. 恐怖の掟が支配する村。謎の遊戯マジャンに勝つしか生き残る道は無い‥! 現代に残る「畏れ村」の因習に怯えつつも少年は尊厳を賭け戦いを挑む。 新感覚麻雀ホラー。 [関連情報] E★エブリスタプレミアムにて、2010年6月4日から連載中 … 100,000+ downloads, Rating 3.88 (450)


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Printing lightweight, flexible, and functional materials
3D printing is revolutionizing the production of lightweight structures, soft robots and flexible electronics, but the technology struggles with complex, multimaterial integration.
3D printing is revolutionizing the production of lightweight structures, soft robots and flexible electronics, but the technology struggles with complex, multimaterial integration.
Researchers at MIT and Boston Children's Hospital have developed a system that can take MRI scans of a patient's heart and, in a matter of hours, convert them into a tangible, physical model that surgeons can use to plan ...
An electrical engineer at Missouri University of Science and Technology is using microwave energy to test concrete and rehabilitated aluminum, and in the future her work could lead to safer bridges and aircraft parts.
In a modern, multicore chip, every core—or processor—has its own small memory cache, where it stores frequently used data. But the chip also has a larger, shared cache, which all the cores can access.
The boiling of water is at the heart of many industrial processes, from the operation of electric power plants to chemical processing and desalination. But the details of what happens on a hot surface as water boils have ...
Researchers from the University of Washington are awaiting the launch an over 50-foot-long rocket from a launch site in Norway into the upper reaches of the atmosphere to observe and measure a puzzling phenomenon.
A new approach that uses light to move mirrors could usher in a new generation of laser technology for a wide range of applications, including remote sensing, self-driving car navigation and 3D biomedical imaging.
A research team at Korea Advanced Institute of Science and Technology (KAIST) has developed fiber-like light emitting diodes, applicable to wearable displays.
Most people are familiar with origami, the ancient paper-folding art form that creates unique patterns and shapes. Less familiar is the fact that origami has inspired the design of engineering devices and structures.
With 2.5 million daily users, the Tor network is the world's most popular system for protecting Internet users' anonymity. For more than a decade, people living under repressive regimes have used Tor to conceal their Web-browsing ...

subset: commoncrawl


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Volcom Going Study Backpack
- All-over abstract print canvas backpack
- Multiple zipper compartments
- Padded adjustable straps
- 100% Polyester.
- Hand wash cold. No bleach. Do not wring or twist. Line dry in shade. Do not iron.
- Made in China.
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Women's Volcom Bags
- Dimension: 11"(L) x 4 1/2"(W) x 18"(H).


text: Hi there im running into a problem. just activated the First data gge4 live account. When I ran a test I got this error message when I clicked the next button "There was a problem with your submission. Errors have been highlighted below." but there is not highlighted information. I believe it has something with the plugin because when I deactivate this gateway and reactivate my old payment gateway everything works as normal. Its really strange because all worked will on the demo account all worked well. Please help


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Does It Get Better???
View Single Post
January 21 2013, 11:06 AM
Dr. Sevrin
Location: Melakon's grave
Re: Does It Get Better???
I visualize the Grim Reaper in the Ingmar Bergman/Woody Allen style, with the black robes, hood, skull face, and a scythe. I played one once in a college production.
My dad had a heart attack in his forties, and was taking nitroglycerine and digitalis, so I think I've been lucky. The only pill I pop is ibuprofen for pain.
I did have an ulcer at 40, but it had been a stressful year leading up to it, no problem like that since.
Dr. Sevrin is insane.
Dr. Sevrin
View Public Profile
Send a private message to Dr. Sevrin
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search for a card
Result of the search for the collectible card:
The following 4 users match with the requested search. Click on their names to contact them.
For some advice on how to trade cards click here.
- genoskill LV5 (Chile-Valparaíso)
- undeeeer LV3 (Chile-Cachapoal)
- belkaster LV2 (Chile-Santiago)
- ioni LV1 (Chile-Santiago)
When you register you will be able to search for all your cards in one go, and we will send you any new results of the search every day by email.


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The Hama soft board cover is specifically designed to protect your new Apple iPad or iPad 2 from knocks and scratches. The black cover is produced from an elegant jacquard fabric and has a hidden front pocket with zip to store accessories. The cover features an integrated plastic board which protects the sensitive display against shocks, and the special fleece inside the cover protects the surface of the device. The padding underneath the zip provides extra protection from scratches.
Catalogue Number: 210-6122
- Protects the iPad 2/3/4 from dirt, dust and scratches on the go
- Integrated shock resistant board for additional protection of the display
- Hidden front pocket with zip for accessories
Sorry, there are currently no sellers for this product.


text: This is the place to try out the original beef noodle. The place, despite its modern look is located exactly at the same spot when it was opened 'reportedly' a century ago. But it is now fully commercialized. You order your dishes according to pre-determined menu: brisket, tenderloins, intestines... read more


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Find many answers to both web related and general questions on our FAQ page.
If you cannot find an answer to your question, please contact the Fjällräven Customer Service by simple using our online form below. Since our customer service team is based in Sweden, please write your request in ENGLISH or SWEDISH. One of our Customer Service Fjällrävs will respond to your queries as quickly as possible.
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Ideal for squeezable condiments, the new Ultra Light closure features a liquid trap that keeps separated liquid inside the bottle. The cap's modern 180° opening promotes an easy-to-open, cleaner, more sanitary orifice area that is positioned to provide improved directional dispensing.
The difficulties in opening different types of packs are of far greater concern to most consumers than environmental criticisms such as perceived over-packaging. This is one of the findings of a major quantitative research study carried out on behalf of packaging specialist Payne.
Cartons deliver four sides of billboard space and a surface perfect for high definition graphics – a blank canvas for brands to tell their story, promote premium product benefits and offer promotions. Evergreen Packaging can help brands get the most from their packaging design with our Shelf Impact Program.
Package and display designcreating a winning synergy
Throughout their career, packaging professionals will work on numerous new products, and for those in the consumer packaged goods industry, it's imperative to understand how and where the consumer will first come into contact with the package.
Redesigned skin-care package overcomes daunting hurdles
The Mederma® family of skin-care products for scars and stretch marks is produced by Merz Pharmaceuticals, LLC, part of the Merz Group of companies whose areas of therapeutic focus include neurology, dermatology, and podiatry.
Branding, marketing, and advertising all converge on the front panel of a retail package. Dedicated package designers would argue a package does all of that and more, and that nothing represents the brand more than the retail package. That's because the package is the last place the consumer interacts with the brand prior to making a purchase decision.
There are certainly no hard-and-fast rules in front-panel package design, and some categories have much more freedom to experiment. But here are some guidelines that will help you define your brand on the front panels of packages on today's cluttered retail shelves.
2. Explore the competitive environment. Use differentiation in a category for one goalgiving consumers a reason to pick up the package. Go to the retail environments where the package will live, and ask these questions from the perspective of the brand:
3. Settle on a hierarchy. Information organization is a critical element of front-panel design. Broadly, the importance of the information hierarchy goes: 1) brand; 2) product; 3) variety; and 4) benefit(s). Analyze all the messages you want to convey and put them in order of importance. This doesn't necessarily mean that they'll be in that order, top to bottom, on the package, but it's a good reference point to start with. Having a very organized, consistent information hierarchy across multiple product varieties helps your customer find the variety they desire and allows for a satisfying experience. Saving the shopper time in picking out a product should always be a priority.
4. Make one element the hero. Is the personality of the brand strong enough to stand on its own? Determine what is the most important single idea to communicate about your product. If you're going to "own" something, what is that something? Align secondary brand messages under the primary umbrella message. If your brand is the hero, consider "locking in" a tagline with the logo. But make sure you're committed to that tagline for the long haul. Otherwise, look for inspiration outside the category, which can often lead to breakout design. Use shapes, colors, illustrations, and photographs to reinforce the hero of your brand story. Above all else, make it easy for repeat buyers to find you the next time.
Read the rest of the tips
Throughout their career, packaging professionals will work on numerous new products, and for those in the consumer packaged goods industry, it's imperative to understand how and where the consumer will first come into contact with the package. The packaging team for every new product dreams of winning at that First Moment of Truththat moment when the consumer interacts for the first time with the package. While there is great focus on the industrial design of the product, as well as the graphics and structural design of the package, equal time should be spent thinking about where this product will launch and live at retail.
At Newell Rubbermaid, in the Writing Segment, there is a strong synergy between creative design and packaging and merchandising engineering. Many new products are first showcased at retail in an off-shelf merchandising vehicle. Therefore, when developing the package, we need to consider the product presentation for both the plan-o-gram and the displays.
Read the full article
Mederma Stretch Marks Therapy's upgraded packaging is lighting up retail shelves and addressing major retail challenges thanks to its branding partner, Little Big Brands.
The hurdles necessary to overcome for the package redesign were daunting. The Mederma Stretch Marks Therapy has one retail facing, and that's usually on the bottom shelf, among a disparate array of skin-care products. The product's $40 price tag is higher than most drugstore products, which earns it security devices in some stores.
The project kicked off with a robust structure exploratory with the goal of keeping the product's current footprint and material costs, while working harder to catch the consumer's eye. It also had to better educate the consumer, while justifying its purchase price.
While today's supermarkets and natural food stores are becoming inundated with kefir varieties, The Greek Gods® brand saw an opportunity to introduce a Kefir line for people who seek the indulgent qualities found in The Greek Gods brand Greek-Style yogurt. Biondo Group, was asked to develop a The Greek Gods Kefir Low Fat Cultured Milk line of packaging to enhance the brand equity of The Greek Gods yogurt, giving the Kefir line a dynamic, modern look. Says Biondo, "We were asked to develop a packaging solution that would be consistent with the flavors and ingredients used in the existing products but also to develop a line with its own personality. After approaching this project from several design directions, we decided that the best way to reinforce the brand's positioning was to highlight the classic beauty of Greek Gods themselves. By utilizing large visages of the GodsAthena, Poseidon, Eros, and Hermeswe established an immediate connection between the product and imagery." The overall white bottles communicate a refreshing feeling, while the pictorials of fresh fruits and honey deliver on the promise of taste and nutrition. The back panel features a short history of The Greek Gods® brand along with romance and mandatory copy.
Cisco has redesigned the packaging graphics for its line of six Linksys WiFi routers, with a kaleidoscope of vibrant visual aids and clearly communicated product capabilities, to provide shelf pop and clarity in a category that had become utilitarian and overly confusing. "Cisco wanted to step up from the category," explains Kara McCartney, strategy insight director for Landor Associates, which led the redesign. Cutting through gratuitous information, Landor developed a streamlined capacity chart for the back panel, modeled on the one used on hosiery packaging. Another of Cisco's goals with the redesign, McCartney adds, was to translate through its packaging, "what a wireless router could potentially be in the future, and how it could really become the heart of the home, moving forward." Communicating this on-pack is a mandala design on the front panel that uses bright splashes of neon color, along with images of compatible devices, such as iPads, laptops, and game controls. The eye-catching color schemes also serve the purpose of organizing the routers by capacity.
Vittel entrusted Logic Design, a French design agency, to create a new identity based on the theme of vitality. The new design is the perfect combination of the vision (consumer benefit), the charisma (graphic language), and the status (institutional dimension) of Vittel. It expresses the vitality of the brand through a symbolic system of gushing water, with a key visual shaped by the V of Vittel and the vertical logo, which forms the brand block. This new brand identity appears on the label and on the shrink film, providing Vittel with more of the dynamism and revitalizing energy that characterize the essence of the brand. The design allows Vittel to express both its promise and status and to assert the brand's power graphically.
Ask our panel of experts your questions about packaging sustainability. The Greener Package Expert Network consists of packaging sustainability experts from major consumer packaged goods companies, independent consultants, academics and non-profits. Confused about sustainability? Get some answers.
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Patient Comments: Cortisone Injection - Knee and Hip
Share your experience with cortisone injections in your hip or knee.
Comment from: Happygrl, 45-54 Female (Patient) Published: October 02
When I first got cortisone injections there was no problem, but a little pain at the injection site. Now when I get the injection 24 hours later I have dry mouth, palpitation, headaches, I just don't feel well at all and this lasts for a week every time now. I am happy I don't have the pain, but the side effects are the worst. My doctor told me that what I am experiencing is not from the injection because this medicine is produced naturally in the body, but they don't happen till I have the shots. I am confused and miserable.
Comment from: 55-64 Female (Patient) Published: September 28
I have pinched nerves on my upper back. I've had cortisone shots before and it was ok. But after the last one I had, on the way home I started to get sick to my stomach and then my head started spinning really fast, scared the life out of me. I don't know why it happened but I'll never have another shot again.
Comment from: the old dog, 75 or over Male (Patient) Published: August 11
After reading some of the after effects of a cortisone injection, I can feel for people that continue to have pain in the knee. Evidently it has to do with the doctor and the placement of the needle. For me, after receiving an injection of cortisone, the pain was gone, and I walked out of the clinic pain free. With a bit of luck the injection will last 6 months, and should it return, I will be able to get another injection. Having the option of a knee replacement or a shot, I went with the shot, glad I did.
Comment from: Linda, 55-64 Female (Patient) Published: July 29
Comment from: shartrandditto, 75 or over Female (Patient) Published: July 28
I have drastic mood swings with my cortisone shot! I got a shot in both knees, thinking I could handle the mood swings. I can't! I got the shots a week ago. My knees feel like Jell-O, not painful but very unsteady. I had insomnia, night and day sweats, and from anger to crying spells. I'll take the pain! I never want to go through this again.
Comment from: alan, 65-74 Male (Caregiver) Published: August 21
I have had three cortisone injections but after the last one my tennis elbow has given me more pain than ever before. My tennis elbow was caused because I fish a lot and have had to stop because of such pain in my arm and neck.
Comment from: fireplug, 55-64 Male (Patient) Published: July 21
I have had about 6 cortisone injections over the past 2-years and I can say the last two have not been as effective as the first four. My doctor says in about five years I will need a replacement. I am not bone on bone yet but getting close. I can feel it mostly going up stairs or twisting my knee unexpectedly, and kneeling is extremely painful. So I would suggest the shots but can't say how long they might work.
Patient Comments
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Cortisone Injection - Experience Question: Please describe your experience with a cortisone injection.
Cortisone Injection - Side Effects Question: What side effects (if any) did you experience with your cortisone injection?
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Men searching for the key to life-long virility, look no further than the nearest grocery store. Specifically, stock up on vegetables, milk, ghee, almonds and chapattis, an Indian flatbread.
That's what a 96-year-old Indian man, whose 52-year-old wife gave birth to a healthy boy on Oct. 5, attributes his vitality to, the Times of India reported. Ramajit Raghav, who lives about 31 miles northwest of Delhi in the district of Haryana, now claims he is the oldest man in the world to father a child — breaking a record he set in November 2010, when his first son Vikramjeet was born. ABC Newswas unable to confirm Raghav's age, but the Times of India reported that Haryana government's social welfare department lists his age as 96.
Raghav, a former wrestler, told the Times of India he also credits his fertility to an active, alcohol-free lifestyle. "I wake up at five in the morning and go to bed before 8 p.m. During the day, I work in the fields and also take [a] one- to two-hour afternoon nap," he said.
Although Raghav and his wife Shakuntala Devi initially planned to limit their family to three after Vikramjeet's birth, he told the Mirror that he is happy to welcome their newborn. "What can I do? This is all God's wish. He wanted me to have another son," Raghav said. "It's good that I have another son now. Even if one of them dies, God forbid, I will have someone to carry on my family name."
But Devi told the Times of India the couple will stop having kids for financial reasons. The Telegraph reported she will undergo a tubectomy this year."My husband earns a living by working in the fields. Besides, he gets Rs 500 [about $9.50] as old age pension from the state government. This is insufficient to feed our children and family," Devi told the newspaper. "We hope to educate them and do something good in life."
Raghav also has high aspirations for his children, telling the Mirror that he wants them to become high-status government officials. The new father recognizes he and his wife must make sacrifices, including their sex life, for their sons' well-being. Raghav told the Mirror that he and Devi "do it three or four times a night," but now their kids are their top priority.
"I'm healthy and I enjoy sex with my wife," he told the Mirror. "I think it's very important for a husband and wife to have sex regularly and when she asks I will go on all night, but for the sake of my child I've put our needs aside for now."
Raghav says he'd remained celibate before he met Devi on a rainy day at a Muslim shrine ab0ut 10 years ago.
"She didn't have any family or friends around, and I wanted to help her," he told the Mirror. "I took her under my wing and taught her some yoga and we fell in love. Many of my past girlfriends had died so I had never married and then I asked Shakuntala to be my wife."
Devi told the Mirror that she remains confident in Raghav as a partner and a father despite his age.
"It doesn't matter how old he is, I love him and I care for him dearly, even though he shouts at me sometimes," she said. "He makes a wonderful father."
Edited by Amaneey, 19 October 2012 - 12:25 AM.


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The open educational resource movement is growing rapidly. Open educational resources give many people who would not be able to study due to finances
Via Dr. Susan Bainbridge
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We Are Social's comprehensive new report covers internet, social media and mobile usage statistics from all over the world. It contains more than 350 infograph…
Via Ana Cristina Pratas
Every time I ask the question "what's the most difficult part of writing a speech?" the answer is always the same. STARTING. Writing a presentation is inherent…
Via Baiba Svenca
Jeff Domansky's curator insight, August 22, 10:26 AM
A very creative design and useful presentation tips.
Presentation software for Mac and iPad. Easiest way to create beautiful presentations.
Via Baiba Svenca
Baiba Svenca's curator insight, September 15, 2:44 PM
FlowVella supports education, designed for work in the classroom, free student accounts.
Former Flowboard app.
Learn more about "The Science of Memorable Presentations" by checking out the Ethos3 blog post on this topic: http://ethr.ee/1ULMrxy Ethos3 is a presentation …
Via Baiba Svenca
Dr. Pamela Rutledge's curator insight, September 16, 10:54 AM
Another way of interpreting these tips is to think of the presentation as telling a story using all the tools at your disposal to focus the listener and engage emotion--structure, image, data and audience need.
Everyone tells stories: journalists, politicians, scientists and entrepreneurs. Conveying information in a coherent and compelling way is vital to success in the real world, and it's our job as educators to prepare our students to share their ideas in effective ways.The emphasis is on empowering st
Via Ana Cristina Pratas
Popular Bingo Sites's comment, September 3, 1:58 PM
Play Bingocams, the only online bingo website where you play, win and meet other people live. Signup today for £5 free and 300% bonus on your first deposit.
The photo essay can be a great way to encourage your reluctant teen writers. And they'll have a reason to use their phones—which, of course, cheers them up!
Via Ana Cristina Pratas
From medium
You can't have a conversation about photojournalism without discussing ethics, so to get the conversation started, we wa…
Via Ana Cristina Pratas
A hand-picked directory of the best free resources for creatives.
From hbr
It's easy to assume that everyone knows how to work on a team — and on some level this is true. But each member of your team probably has her own understanding of how to collaborate, not to mention individual styles and preferences.
Body language and especially HAND gestures make speakers' oral presentations more engaging, memorable, and successful: http://www.scienceofpeople.com/ted/
Teachers TV - Body Language video.
Learn how to distinguish negative from positive body language in a sales sitaution.
Take it from someone who hates boring powerpoints. If you apply these 4 takeaways you'll look like a professional presenter. Or you can have your money back - …
Via Baiba Svenca
Jimena Acebes Sevilla's curator insight, August 9, 4:22 PM
Tíos para hacer presentaciones "modernas" en PowerPoint.
Costantino Soudaz's curator insight, August 9, 11:58 PM
Watch this constructive and matter-of-fact presentation with your students and learn some basic rules that have to be kept in mind while creating a winning PPT presentation.
WIKIBLINKS's curator insight, August 10, 12:14 AM
Watch this constructive and matter-of-fact presentation with your students and learn some basic rules that have to be kept in mind while creating a winning PPT presentation.
Most colleges and business schools use a Presentation Rubric to evaluate their students. Funny thing is, they're not usually that open about sharing it with their students (as if that would do any harm!)
Via Baiba Svenca
Christophe Poupet's curator insight, August 30, 12:24 PM
une grille d'évaluation intéressante sur nos présentations/diaporamas...
Ricard Garcia's curator insight, September 1, 5:15 AM
Showing what we expect from them is PARAMOUNT!!!!
Need to prepare a last minute presentation? Learn the tips to given powerful presentation when you have little time to prepare.
Via Baiba Svenca
Baiba Svenca's curator insight, September 1, 6:07 AM
There are not many people who haven't experienced a situation when you have a minute left till your presentation goes on air. How to cope? Watch this slide presentation to learn the tricks.
Giving a presentation? Not sure where to get started? The first step is to break the rules that you've always been told. Human attention spans are shorter
Learning Mind: Blog with lots of interesting and useful information on science, self improvement, psychology, world mysteries and many more!
Via Ana Cristina Pratas
Trackography is our open source project which illustrates which companies track us when we read the news online, as well as where our data travels to everytime we access a media website within a period of time.
Via Ana Cristina Pratas
Gust MEES's curator insight, September 7, 12:48 PM
Trackography is our open source project which illustrates which companies track us when we read the news online, as well as where our data travels to everytime we access a media website within a period of time.
Learn more:
Among the common misconceptions in education is that students are comfortable with technology, and therefore implementing blended learning doesn't require…
Via Ana Cristina Pratas
Lon Woodbury's curator insight, September 11, 12:06 AM
Makes sense. Just because some students are whizzes in the new technology doesn't mean that a lot of them are faking it. -Lon
Creating posters has been a staple of so many classrooms of different ages. Usually, it entails gathering some important supplies before starting — posterboard, glue, markers, etc. Many teachers will round up old magazines, too, letting students cut and attach images that illustrate their topics. The digital age has opened classrooms up to a [...]
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15 things that you really should know about presentations! from SOAP – State of the art presentations
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Clayton Homes
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Clayton Homes is a business providing services in the field of Mobile Homes - Dealers. Clayton Homes is located in Conway, AR on 1200 South Amity Road.
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News from the Left Coast: California Bans Plastic Bags
It was bound to happen, and it finally has. On September 30, 2014, Governor Jerry Brown signed into law a bill that makes California the first state in the country to prohibit stores from offering environmentally-problematic single-use plastic bags to their customers. SB270 provides that plastic bags will be phased out from use at supermarkets, large grocery chains and pharmacies..


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The problem is that I need a little extra functionality to an object of a class that I can't change (I'm trying to add data binding support). The best solution that I can think of is to just write a derived class with this functionality. So I can use objects of this class instate. So now the problem is, how do I initialize the objects of the new class? I could make a constructor with the original object as a parameter and initialize the derived object with the values of this object, but to me this seems not to be the smartest solution. It would be nice if I could do something like:
// MyDerivedClass is derived from ObjectOfAnUnchangeableClass.
MyDerivedClass Obj = ObjectOfAnUnchangeableClass as MyDerivedClass;
Of cause this would not work because the ObjectOfAnUnchangeableClass does not know of MyDerivedClass. Another idea would be to have a constructor that could be "initialized" with an object. Something like:
public MyDerivedClass(UnchangeableClass obj): base(obj){}
Here the idea would be that instead of having the base constructor build a new object; it could just take the existing object.
So I have two questions:
- Is there some concept in .net the supports something like mentioned above?
- What would be the best solution to have some extra functionality in a class that can't be changed?


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Families with special needs children are celebrating after an outpouring of community support is allowing a valuable resource to stay open in Oakland County.
Families with special needs children are celebrating after an outpouring of community support is allowing a valuable resource to stay open in Oakland County.
A financially strapped nonprofit center that provided a break for parents of disabled kids will be staying open. J
CW50 Streat Beat - aired on Saturday, September 12, 2015.
Foster Care Navigators, Tammy Gabourie of Judson Center and Melissa Covell of Child Safe (an affiliate of Judson Center) discuss Foster Care and the need for families.
Katie and Dave Andrea of Troy know exactly what the Judson Center's Lahser Respite Home has brought to their lives:
The yearlong affiliation between the human services agency and OU's School of Education and Human Services aims to improve services for those with autism spectrum disorders and other disabilities.
Judson Center officials announced last week that their Lahser Respite Home, in Beverly Hills, will remain fully operational for the upcoming year. The announcement was made three months after news
Lahser Respite home is very close to their goal. For news story: www.youtube.com/watch?v=t-Xb2DA1i5M
Lizzie cannot converse.She cannot care for her
Livonia resident Larry Stock and 122 other motorcyclists had picture perfect motorcycle riding weather Saturday for their Annual Ride for Autism, which raised more than $15,000 for autistic children and their families.
On Saturday, August 1, motorcycle enthusiasts and autism advocates alike attended the 11th annual Ride for Autism.


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IN PERSON; From Agitated To Agitator To Litigator
Published: August 31, 2003
(Page 2 of 2)
''I stood there with my two rocks,'' he said. ''And those fools said, 'You get him Charlie!' And I hit him right in the head. And he started bleeding and crying. And I said, 'Now who else wants to fight me tonight?' And nobody else wanted to fight me that night, and I didn't have anymore trouble after that.''
To this day, Mr. Harris is surprised he remembers the incident in such detail.
After graduating from high school, Mr. Harris attended Pennsylvania State University, where he joined the Black Student Union. As one of only about 200 blacks on campus, he agitated in the turbulent spring of 1969 to increase the number of minority students. He and a group of other students headed toward the president's offices, bricks in hand -- and built a wall near the entrance of the building as a symbol of what they felt was a barrier, occupying it for the day and eventually negotiating concessions.
Upon graduation, Mr. Harris did social work in rural Pennsylvania before taking a job at Brooklyn College, where he was told by an attorney for the school that he should seriously consider law school.
After taking night classes and graduating from Rutgers, he found his way to the unlikely offices of Lowenstein Sandler, which was then situated in Newark. He actually had his hopes pinned on civil rights work.
Yet he rose through the ranks and was made a partner in 1985, the second black in the firm to do so. In 1993, a colleague recommended Mr. Harris to Senator Bradley to fill a vacancy on the federal bench, though he eventually withdrew his name because of concerns that he would not be approved by the Republican-controlled Senate.
As for the case against the Division of Youth and Family Services, he admittedly took a less active role in the past few years. Still, Mr. Harris was instrumental in drafting the suit, and says he was a bit shocked by the settlement.
''They have given away control,'' he said. ''And it is surprising.'' He attributes the settlement to the horror stories that have come out, beginning with the discovery of a dead child, Faheem Williams, and his two malnourished brothers in a Newark basement in January.
''You're talking about an entire state, an entire system that has been doing its job so poorly for so long that it's devastating,'' he said. ''How long will it take to get it right? I think it's going to take 20 years.''
In a case that he lost earlier this year, Mr. Harris took on Thomas S. Howard, a lawyer from Hackensack who was representing two shareholders who believed that they had been unfairly barred from selling stock as the share price of a company plummeted. Mr. Harris was brought in on the eve of a trial, and lost the case - the jury awarded $7.6 million to the plaintiffs. Still, Mr. Howard was impressed with Mr. Harris.
''He's very creative with his legal arguments,'' he said
Over the years, Mr. Harris has written about the trouble that minority attorneys face in trying to advance although his boss, Mr. Rodburg, does see change. He cited an example at his own firm in which a black lawyer was assigned to a high-profile black client who became increasingly dissatisfied. As Mr. Rodburg tells it, the client said, ''I love black, but I love green more.''
As Mr. Harris prepares to head the litigation department - about 100 attorneys will be under his supervision - he says he is satisfied yet bewildered at his status as a groundbreaker.
''I'm tired of being first,'' he said. ''It's 2003. I shouldn't be first.''
Photo: David L. Harris, a partner at Lowenstein Sandler, took on the state's problem-plagued Division of Youth and Family Services. (Photo by Timothy Ivy for The New York Times)


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In the last several months, Interfaith Neighbors has seen a great level of need as a result of Hurricane Sandy. Our area has been devastated, and families are falling through the cracks who didn't have insurance or who are ineligible for relief from FEMA. As a result, Interfaith Neighbors established a Sandy Relief fund to support the most vulnerable residents, and to help them back on their feet. Last month, Interfaith Neighbors provided assistance a single mother of two sons. Because of the storm, the mother's employment stopped for several weeks which caused a financial hardship. The mother's employment has resumed and the family is most grateful for the assistance. If you would like to help people just like this, please consider making a donation. Thank you!
Project Reports on GlobalGiving are posted directly to globalgiving.org by Project Leaders as they are completed, generally every 3-4 months. To protect the integrity of these documents, GlobalGiving does not alter them; therefore you may find some language or formatting issues.
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Formula feeding babies
He drinks about 5 ounces every 3-4 hours. At his 2 month well check last week he was 11lbs 14 oz. He is 10 weeks old.
At 8 weeks old, he drinks 4 oz every couple of hours in the daytime, and every 3-4 hours at night....
Loving being a mommy to Mason since 1/30/12, and wife to Damon since 3/18/11! Also have 3 DDogs Angel, JC, & Ollie
he averages 20-27 ounces per day. My sheet from doc told me anywhere from 18oz to 32oz per day is normal for a 2 month old baby.
Primarily ff, gets like 1-2 bottles of bm a day. He was 13 lbs, 5 oz at 2 month check up, he is 11 weeks now. He eats about 4-5 oz every 3-4 hours.
Love of my life 7 years strong Brittany (26) DH (30) =DS 1/5/12 8 lbs, 13 oz.
My LO is ten weeks and drinks 5 oz. every 2.5-3.5 hours during the day. His last bottle before bedtime is 6oz. and his first bottle when he wakes up is 6oz. He usually sleeps about 10 hours straight at night.
Proud Mommy:
"Girls are 50% of the population. We deserve to represent 50% of the heroes."
"Girls are 50% of the population. We deserve to represent 50% of the heroes."
Sabrina + Merv= Ethan (01-01-2012) Shane (06-30-2015)


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Willis, Katherine "Mutz' Ann
Katherine "Mutz" Ann Willis, 80, of Fond du Lac, passed away Thursday, January 24, 2013, at the Fond du Lac Lutheran Home.
She was born on February 26, 1932, in Osceola, Wisconsin, the daughter of Martin and Katherine Schwind Tonn.
On April 7, 1951, Katherine married Lige "Bob" Willis in Ogden, Utah.
Katherine was employed at Infant Socks, Rolling Meadows Nursing Home, and Dairy Queen. She then became a full time stay at home mom to raise her six children. She returned to the workforce, at the young age of 60, to join the team at Goodwill Industries.
Survivors include five daughters and one son, Rebecca (Greg) Harding of Fond du Lac; Mark (Tina) Willis of North Fond du Lac; Cynthia (Roland) Lemke of Hayward; Brenda (DuWayne) Woelfel of Fond du Lac; Carmen (Randall) Bonlender of Lomira; and Margaret Willis of Appleton; 17 grandchildren; 22 great- grandchildren; a sister, Mary Scannell.
She was preceded in death by her parents; her husband, Bob, on March 2, 1992; two brothers, three sisters; and two sons-in-law, Jay Kremer (Cynthia Lemke) and Gene K. Maloney (Brenda Woelfel).
A Mass of Christian Burial will be celebrated at 11 a.m. on Thursday, January 31, 2013, at Holy Family Parish, 271 Fourth Street Way, Fond du Lac.
Katherine's family will receive friends and relatives from 4 to 7 p.m. on Wednesday, January 30, 2013, at Mach Funeral Chapel & Crematory, 31 E. Division Street, Fond du Lac, and on Thursday at church from 10 a.m. until the time of service. Burial will be in Calvary Cemetery.
Katherine's family is grateful to all the staff members of the Fond du Lac Lutheran Home, Agnesian Hospice, the ICU and the 5th Floor of St. Agnes Hospital for their compassionate care for Katherine and her family.
Katherine's family extends their heartfelt appreciation to Heather Herbeck, granddaughter of Katherine, for the care, compassion and kindness she gave and shared with her grandmother over the past months. The companionship, comfort, and consideration provided by Heather can only be matched by the love she had for her grandmother.
Mach Funeral Chapel & Crematory is serving the family,
Published in Fond du Lac Reporter on Jan. 27, 2013
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Book your room at Nichols Airport Hotel
Superior RoomMax Occupancy: 2 guests (up to 1 child, 1 infant)
Premier RoomMax Occupancy: 2 guests (up to 1 child, 1 infant)
Family Room (Four)Max Occupancy: 4 guests (up to 3 children, 3 infants)
Family Room (Three)Max Occupancy: 3 guests (up to 2 children, 2 infants)
Get an overview of this hotel3-star family-friendly hotel near SM Mall of Asia
This family-friendly Paranaque hotel is located near the airport, within 2 mi (3 km) of Baclaran Church and SM Mall of Asia. St. Andrew's Cathedral and Mall of Asia Arena are also within 2 mi (3 km).
Hotel Features
Along with 2 restaurants, this hotel has a bar/lounge and a coffee shop/café. WiFi in public areas is free. Other amenities include a rooftop terrace, a 24-hour business center, and coffee/tea in the lobby.
Room Amenities
All rooms are soundproofed and offer 24-hour room service and free local calls. TVs come with cable channels, and other amenities that guests will find include in-room massages and showers.Information missing or incorrect? Tell us!
Hotel Amenities
Hotel Amenities
Dining is available at one of the hotel's 2 restaurants and guests can grab coffee at the coffee shop/café. A bar/lounge is on site where guests can unwind with a drink. Business-related amenities at this 3-star property consist of a 24-hour business center and a meeting room.
Public areas are equipped with complimentary high-speed wireless Internet access. This family-friendly hotel also offers a rooftop terrace, spa services, and tour/ticket assistance. For a surcharge, the property provides a roundtrip airport shuttle (available 24 hours), an area shuttle, and a casino shuttle.
Nichols Airport Hotel has designated areas for smoking.
- 24-hour business center
- 24-hour front desk
- Airport transportation (surcharge)
- Area shuttle (surcharge)
- Bar/lounge
- Breakfast available (surcharge)
- Casino shuttle (surcharge)
- Coffee shop or café
- Coffee/tea in lobby
- Free newspapers in lobby
- Concierge services
- Designated smoking areas
- Designated smoking areas (fines apply)
- Dry cleaning/laundry service
- Elevator/lift
- Ferry terminal shuttle (surcharge)
- Free WiFi
- Luggage storage
- Number of buildings/towers - 1
- Number of floors - 8
- Number of restaurants - 2
- One meeting room
- Parking (limited spaces)
- Porter/bellhop
- Rooftop terrace
- Safe-deposit box at front desk
- Shopping center shuttle (surcharge)
- Spa services on site
- Spa treatment room(s)
- Television in lobby
- Total number of rooms - 65
- Tours/ticket assistance
- Wedding services
- Year Built 2000
Family Friendly Amenities
- Free Wi-Fi
- Rollaway/extra beds available
- Soundproofed rooms
Available in some public areas: Free WiFi
Parking (limited spaces)
Room Amenities
- Air conditioning
- Cable TV service
- Free toiletries
- Cribs/infant beds available
- Daily housekeeping
- Desk
- Free local calls
- In-room massage available
- Individually furnished
- Phone
- Private bathroom
- Rollaway/extra beds available
- Room service (24 hours)
- Shower only
- Soundproofed rooms
- Television
Where to Eat
Full breakfasts are available for a surcharge and are served each morning.
The Lobby Coffee Shop - This coffee shop serves breakfast, brunch, lunch, and dinner.
24-hour room service is available.
Spa Services
- Couples treatment rooms
- Aromatherapy treatments
To make requests for specific accessibility needs, complete the "Special or Accessibility Requests" section of "Trip Preferences" when you book your hotel.
- In-room accessibility
- Accessible bathroom
- Accessible path of travel
Hotel Policies
Check-in time starts at 1 PM
Check-out time is noon
Payment types
Children and extra beds
- Children are welcome.
- Kids stay free! Children under 12 years old stay free when using existing bedding.
- Rollaway/extra beds are available.
- Cribs (infant beds) are available.
You need to know
Extra-person charges may apply and vary depending on hotel policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
Optional extras
- Fee for full breakfast: PHP 200 per person (approximately)
- Airport shuttle fee: PHP 300 per vehicle (roundtrip)
- Deposit: PHP 1000 per night
- Rollaway beds are available for an additional fee
We should mention
This property offers transfers from the ferry terminal and airport (surcharges may apply). Guests must contact the property with arrival details 72 hours prior to arrival, using the contact information on the booking confirmation. Reservations are required for massage services. Reservations can be made by contacting the hotel prior to arrival, using the contact information on the booking confirmation. Children 12 years old and younger stay free when occupying the parent or guardian's room, using existing bedding. Only registered guests are allowed in the guestrooms.


text: First, don't go during kids vacation time in Mexico, 100% occupancy is terrible due to poor amenities, like only 2 elevators for hundred of people that don't use it properly, no AC in any common area, 98* w/100% humidity is very bad for that, spend up to 20 min. to come dn. from floor 12th. Front desk guy Jared loves... More


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A quiet place to stay in a big house with 5 rooms ,2 floors, 2 bathroom very equiped, 2 terrazes (one very private), dinning room, tv satelit,s, full equiped kitchen, common garden with pool, kettle service in every roon, ameties avalaible, minibar and tv in some rooms, also bykes avalaible free.
Posibility of transfer to the airpoirt.
i am a single gay , working as a hotel manager , very friendly and helpfull, guys you are very welcome to visit my place, you won't regre it. Have a save trip and see us very soon.


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Obituary & Guest Book Preview for Dr. William Joseph Acuff
Dr. William Joseph Acuff Obituary
Published in Knoxville News Sentinel from Mar. 17 to Mar. 19, 2012
First 25 of 557 words
: ACUFF, DR. WILLIAM JOSEPH - age 81, passed away peacefully Friday morning, March 16, 2012, at his beloved home and farm in South Knoxville. He...
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In the fall of 1940, the entire population of Friar, NH abandoned their homes and walked up an ancient trail, never to be seen alive again. Their fates have remained a mystery for over 70 years; until a team of researchers discover the trailhead and attempt to track the path the doomed citizens of Friar took.
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This is a very creepy, unsettling film. There's no gore to speak of but there is plenty of violence. Read morePublished 25 days ago by Micah, the movie lover
This is a terrible movie, plot is hard to follow, first hour is people camping hearing noises and shaking, the first kill is absolutely the most far fetched thing Iv seen in a... Read morePublished 1 month ago by Omegatheundying
I love this movie, have seen it 20 times was excited when it was on Amazon streaming :)Published 1 month ago by Amazon Customer
Great beginning... About half way through the whole thing fell apartPublished 3 months ago by sindpifer
Well acted, written and conceived -- artful (as in art film), interesting, intriguing and relatively novel. Mitton, Holland, cast and crew should be proud of this. Read morePublished 4 months ago by The Mysterious Mr. E.
The first time I watched this movie I hated it. I absolutely HATED it. The second time I watched it...it stuck with me. Read morePublished 4 months ago by Lauren Achen
One of my favorite horror/thriller/suspense movies of all time! You will be at the edge of your seat by the end of the movie. Read morePublished 5 months ago by Kpall
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make sense, thank you... i guess soaked almonds needs to dry first, OK!
Rating5/5 (from 1 ratings)5
YieldServes 4
1 box Fresh organic strawberries
½ box Fresh raspberries
1 Large banana
1 cup Almond yogurt
Recipe Directions
Wash and hull the strawberries then cut in half. Wash the raspberries and drain well. Pat dry on kitchen paper.
Puree half the strawberries and raspberries plus the banana in a blender.
Tip the yogurt into a bowl and gently stir in 3 tbsp of the fruit sauce.
Spoon the reserved strawberries and raspberries, fruit sauce and the yogurt mixture alternately into glasses or dessert dishes.
Chill for up to 3 hours before serving. Decorate with fresh mint leaves.
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Dear Zhanna8,
You may grind the almonds into a fine powder, using either:
* A high power blender
* A coffee grinder
* A food processor
This looks delicious! Thanks for sharing this with us. I am going to try it out this week.
Oh that is so pretty :c)
...and how do you grind inot a fine powder?
raw yogurt--sounds yummy!
MMM this sounds yummy! Going to try it this weekend
There are many variations but this is the one I use:
1/2 cup soaked almonds peeled.
Once dry - grind into a fine powder.
Add 1/2 cup spring water to blend - blend until creamy.
Chill for at least one hour before serving. Stir well
* You can add a little lemon juice for a tarter taste
* You can add a little raw honey or agave for a sweeter taste
How do you make almond yogurt?
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text:
Thomas Babington Macaulay, Baron Macaulay
1 portrait of Thomas Babington Macaulay, Baron Macaulay
© National Portrait Gallery, London
Thomas Babington Macaulay, Baron Macaulay
by Maull & Polyblank, published by Adam & Charles Black
albumen print, published 1863 (April 1856)
4 3/8 in. x 3 1/4 in. (112 mm x 83 mm) image size
Purchased, 1978
Photographs Collection
NPG Ax7346
Click on the links below to find out more:
Sitterback to top
- Thomas Babington Macaulay, Baron Macaulay (1800-1859), Historian, poet and politician; MP for Calne, Leeds and Edinburgh. Sitter in 26 portraits.
Artistsback to top
- Adam & Charles Black. Artist associated with 1 portrait.
- Maull & Polyblank (active 1854-1865). Artist associated with 336 portraits.
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October: Domestic violence awareness month
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A young man from Ogallala was killed Tuesday afternoon when his pickup ran into a semi-trailer on I-80 about four miles west of North Platte.
The collision knocked the trailer off its tandem axle, according to the accident report.
The wreck occurred at 2:34 p.m. Both westbound lanes of I-80 were closed soon after until the wreckage was cleaned up about 4 p.m., a state patrol spokesperson said.
The semi-truck was from Florida. The driver was uninjured.
It is not known what caused Dovel's pickup to cross the median, the spokesperson said.
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After much speculation and many rumors that he would go to the Jets, Nnamdi Asomguha has signed with the Philadelphia Eagles on a 5 year/60 million dollar deal. This helps sure up one of the Eagles biggest problems from last year, the secondary, and makes them an immediate Super Bowl favorite. This is the second time that a Phili sports team has swooped in and grabbed the best free agent away from a NY team's grasp (Cliff Lee), and now makes Asomguha hated to almost all NY football fans.
a terrible blight on our fine country


text:
I want to create an (game based) iPhone application which sends your GPS location on a specific time (like 3-5 times a day) to a server. I found an apple page explaining some functionality to run in the background like location, music and VOIP.
I need the GPS to be accurate on the meter.
Can someone help me with a small example?


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There is agreement that the infectivity assay with the duck hepatitis B virus (DHBV) is a suitable surrogate test to validate disinfectants for hepatitis B virucidal activity. However, since this test is not widely used, information is necessary whether disinfectants with limited virucidal activity also inactivate DHBV. In general, disinfectants with limited virucidal activity are used for skin and sensitive surfaces while agents with full activity are more aggressive. The present study compares the activity of five different biocides against DHBV and the classical test virus for limited virucidal activity, the vaccinia virus strain Lister Elstree (VACV) or the modified vaccinia Ankara strain (MVA).
Virucidal assay was performed as suspension test according to the German DVV/RKI guideline. Duck hepatitis B virus obtained from congenitally infected Peking ducks was propagated in primary duck embryonic hepatocytes and was detected by indirect immunofluorescent antigen staining.
The DHBV was inactivated by the use of 40% ethanol within 1-min and 30% isopropanol within 2-min exposure. In comparison, 40% ethanol within 2-min and 40% isopropanol within 1-min exposure were effective against VACV/MVA. These alcohols only have limited virucidal activity, while the following agents have full activity. 0.01% peracetic acid inactivated DHBV within 2 min and a concentration of 0.005% had virucidal efficacy against VACV/MVA within 1 min. After 2-min exposure, 0.05% glutardialdehyde showed a comparable activity against DHBV and VACV/MVA. This is also the case for 0.7% formaldehyde after a contact time of 30 min.
Duck hepatitis B virus is at least as sensitive to limited virucidal activity as VACV/MVA. Peracetic acid is less effective against DHBV, while the alcohols are less effective against VACV/MVA. It can be expected that in absence of more direct tests the results may be extrapolated to HBV.
Hepatitis B is one of the major public health problems worldwide since approximately 350 million, i.e. 5% of the total population, are infected chronically [1]. An important way to prevent infections encompasses disinfection measures to interrupt transmission by the inactivation of the virus on instruments, surfaces and in biological materials [2]. The hepatitis B virus (HBV) can be inactivated effectively inter alia by chemical biocides characterized by broad-spectrum virucidal activity according to the norm EN 14476:2007 [3]. However, the number of agents achieving broad-spectrum virucidal efficacy is limited and they are not required for the inactivation of HBV such as other human blood-borne viruses since these lipophilic viruses with an envelope are characterized by lower stability than viruses without envelope [4]. Thus, a German guideline for testing the virucidal activity of chemical disinfectants in the human medical area [5] differentiates between disinfectants with virucidal activity effective against non-enveloped plus enveloped viruses and disinfectants with limited virucidal activity exclusively effective against enveloped viruses. Limited virucidal activity has been declared when there is a proven efficacy against two representatives of enveloped viruses, the vaccinia virus strain Lister Elstree (VACV) or the modified vaccinia Ankara strain (MVA) [6] and the bovine viral diarrhea virus (BVDV) strain NADL. Even though biocides with limited virucidal activity can be assumed to be effective against HBV, an efficacy cannot be predicted reliably and, therefore, the virucidal efficacy against HBV has to be validated by the use of robust laboratory methods.
So far, the virucidal efficacy of biocides against HBV can be stringently determined only in vivo by chimpanzee infection assays [7] with less sensitivity or by the use of primary hepatocyte cultures derived from Tupaias, small-squirrel-like animals living in Southeast Asia [8]. However, while animal protection and economic reasons prohibit the use of higher primates for routine tests on commercial products, the Tupaia model is expensive, the availability of hepatocytes is limited and the test requires human sera with high viral load. Furthermore, surrogate assays for measuring integrity of viral DNA [9], activity of viral DNA polymerase [10], reactivity of HBV surface antigen [11] and the physical integrity of infectious viral particles [12] do not correlate reliably with the infectivity of HBV. In addition, the use of the hepatoma cell line HepG2 [13] is very doubtful. However, redifferentiated Hepa RG cells [14] are well accepted and reproducible as HBV infectivity system, but they have not been applied for testing the hepatitis B virucidal activity of biocides. Thus, the most promising and feasible assay is at present the use of a taxonomically related surrogate virus of the same virus family Hepadnaviridae, namely, the duck hepatitis B virus (DHBV), which can be propagated in ducklings or in primary duck embryonic hepatocytes [15,16]. Duck hepatitis B virus shares many physical properties with the closely related HBV [17] and similar inactivation kinetics by disinfectants compared with chimpanzee transmission studies of HBV have been reported [7]. In Germany, the infection of primary duck embryonic hepatocytes with DHBV was established and evaluated for virucidal testing in several studies [18-20].
The objective of the present study was to compare the limited virucidal activity of different biocides, which are often used as ingredients in disinfectants, against DHBV and VACV/MVA. The results should provide information whether the virucidal activity of biocides against VACV/MVA, the most important model viruses for the determination of limited virucidal activity, includes the inactivation of DHBV.
Primary duck embryonic hepatocytes were prepared from liver tissue as described previously [18-20]. Briefly, fertilized Peking duck eggs obtained from the poultry farm Pötzsch (Ostrau, Germany) were incubated at 37.8°C for 21 days. The liver tissue was harvested from 6–7 embryos under sterile conditions, pooled, minced and washed subsequently with phosphate-buffered saline (PBS) supplemented with 1 mg/ml glucose. To ensure the absence of DHBV in the source tissue, the first wash fluid containing blood and liver cells was examined for DHBV DNA by using a qualitative polymerase chain reaction technique [18]. Thereafter, the liver tissue was digested with stirring in the dark using a solution comprising of one volume 0.05% trypsin plus 0.02% ethyl diamine tetraacetate (EDTA) solution (Invitrogen, Karlsruhe, Germany) and 4 volumes PBS plus 1 mg/ml glucose and the resulting cell suspension was removed every 30 min. By the addition of 5% fetal calf serum (FCS, PAA, Pasching, Austria), digestion was inhibited and the cells were collected by centrifugation. After re-suspension in growth medium, cells were stored at 4°C. Digestion of the remaining tissue was continued using fresh trypsin/EDTA solution.
DHBV-negative cells were seeded into 24-well culture plates (CellBIND®, Corning, Acton, United States) with a seeding density of about 0.8 × 106 cells per ml and 1 ml per well. The growth medium consisted of Williams medium E (Invitrogen) supplemented with 1.5% Me2SO (Sigma-Aldrich, Taufkirchen, Germany), 15 mM HEPES (Invitrogen), 1% insulin-transferrin-selenium solution (Invitrogen), 10 μM hydrocortisone 21-hemisuccinate (Sigma–Aldrich), 1 mg/ml bovine serum albumin (Sigma-Aldrich), 2 mM L-glutamine (Lonza, Verviers, Belgium), 0.1 μg/ml fungizone (Lonza), 100 U/ml penicillin (Lonza) and 100 U/ml streptomycin (Lonza). For cultivating, cells were incubated at 37°C in a humidified atmosphere containing 5% CO2 and the growth medium was replaced after 24 h and on day 4.
Experimental investigations on embryonated hens' or ducks' eggs are, in general, regarded as borderline trials between in vivo and in vitro systems and do not conflict with ethical and legal aspects of animal protection [21]. Therefore, the experiments of this study are not subject to the "Animal Research: Reporting In Vivo Experiments" (ARRIVE) guidelines and ethical approval was not necessary.
DHBV-containing serum from congenitally infected ducks was kindly provided by Prof. M. Nassal (University Hospital Freiburg, Department of Internal Medicine II/Molecular Biology, Freiburg, Germany). Sera contained between 106.0 and 107.13 tissue culture infective doses 50% (TCID50) of DHBV per ml corresponding to 108.77 and 109.90 DHBV genomic copies. After preparation of 0.5-ml aliquots, sera were stored at −80°C.
Following biocides were used in this study: (i) ethanol (30%, 40%, 50%, 60%, v/v; contact time: 1 min, 2 min), (ii) isopropanol (20%, 30%, 40%, v/v; contact time: 1 min, 2 min), (iii) peracetic acid (0.001%, 0.005%, 0.01%, 0.05%, v/v; contact time: 1 min, 2 min), (iv) glutardialdehyde (0.05%, 0.1%, w/w; pH 8.4; contact time: 0.5 min, 2 min, 5 min), (v) formaldehyde (0.7%, w/v; pH 7.0; contact time: 5 min, 30 min, 60 min). The pH values of the biocides were measured if necessary.
Test samples for the proficiency panel (stock solutions) were generated by an independent provider (kindly provided by Dr. F. von Rheinbaben, Ecolab Deutschland GmbH, Düsseldorf, Germany). All samples were sent out by a commercial parcel service and arrived 24 h of dispatch.
In order to determine hepatocytotoxicity, biocides were serially diluted tenfold in growth medium up to a dilution of 10-8. One part by volume of water of standardized hardness instead of DHBV-positive serum was mixed with one part by volume of FCS as organic load and eight parts by volume of the biocide. Aliquots of 100 μl from each test concentration and each dilution were then inoculated into two wells of a 24-well micro-titer plate containing monolayer of primary embryonic duck hepatocytes plated 4 to 5 days before. The cells were incubated at 37°C for 7–10 days and examined as described in the chapter "Immunofluorescent DHBV antigen staining". Cytotoxicity was evident when cell monolayers were detached from the growth surface, hepatocytes became round and lost their typical polygonal morphology.
Tests were carried out in accordance with the DVV/RKI guideline including minor modifications [5]. One part by volume of DHBV-containing duck serum and one part by volume of FCS were mixed with eight parts by volume of the biocide in a 1.25 fold pre-dilution of the concentration to be tested. The tests were carried out in ambient temperatures of 20-22°C. At the end of the chosen exposure time, the test compounds were removed immediately from the mixture of virus and test formulation by the use of MicroSpinTM S-400 HR Columns (GE Healthcare UK Limited Little Chalfont, Buckinghamshire, UK). To this end, 125 μl of the test mixture was added to the MicroSpinTM column and centrifuged at 737 g for 1 min. Subsequently, 120 μl of the eluted suspension was mixed with ice-cold cell culture medium to obtain a virus dilution of 10-1.7 and to prepare serial ten-fold dilutions up to 10-7.7. Without delay, 500 μl of each were seeded in one well of 24-well micro-titer plates containing monolayers of primary duck embryonic hepatocytes at day 4 of cultivation and 50 μl cell culture medium. This resulted in final viral dilutions from 10-2 to 10-8. Afterwards, the cells were incubated at 37°C for 7–10 days.
Indirect immunofluorescent antigen staining was employed for detection of DHBV surface antigen as described previously [18,19]. In short, the cells were washed once in PBS and fixed with acetone/methanol (1:1) at −20°C for 10 min. After air drying, cells were re-hydrated in PBS for 5 min and incubated with polyvalent rabbit anti-DHBs (kindly provided by Dr. D. Glebe, Institute of Medical Virology, National Reference Centre for Hepatitis B and D, Justus Liebig University, Giessen, Germany) antiserum diluted 1:100 for 1 h at 37°C, washed three times whith PBS and stained with goat anti-rabbit IgG Alexa Fluor® 488-labeled antibody (Life Technologies, Darmstadt, Germany) diluted 1:400 in PBS containing 0.001% Evans blue and Tween 80. Stained cells were analyzed using the fluorescent microscope Nikon DIAPHOT-TMD (Nikon-Corporation, Tokyo, Japan). The total number of positive cells per well was estimated visually.
Each experiment was performed at least eight times, maximally twelve times. From the total number of positive cells per well (maximally 40% infected cells), the virus titers of each test and the corresponding virus control (not treated with biocide) were calculated. Titer reduction is presented as the difference between the control virus titer and the virus titer after contact time with the biocide. This difference is given as reduction factor (RF) including its 95% confidence interval (CI). A reduction of infectivity of ≥4 log10 steps (inactivation ≥99.99%) was regarded as evidence of sufficient virucidal activity. The calculation was performed most widely according to the DVV/RKI guidelines [5].
The detailed results of virucidal testing of the five different biocide ingredients against DHBV are summarized in Tables Tables11 and and2.2. For comparison, these tables contain the results of testing these biocides against VACV and MVA published previously by Rabenau et al. [6]. After exposure of DHBV to 40%, 50% and 60% ethanol, ≥4 log10 reductions in viral titers were achieved after time intervals of 1 min and 2 min. By contrast, the use of 30% ethanol was associated with RFs of 2.99±0.78 after 1-min and 3.46±0.64 after 2-min exposure. These findings corresponded widely to the inactivation of VACV and MVA, but a sufficient activity was detected for 40% ethanol within 2-min exposure. The use of 20% isopropanol resulted in RFs for DHBV within 1.96±0.66 after 1 min and 2.33±0.61 after 2 min. Whereas 30% isopropanol after 1-min exposure did also not result in sufficient activity (RF 3.39±0.88), there was sufficient activity when 30% isopropanol was used for 2 min and 40% isopropanol for at least 1 min. In comparison, 40% isopropanol for at least 1 min was necessary to inactivate VACV/MVA. Peracetic acid at a concentration of 0.01% inactivated the infectivity of DHBV after 2-min exposure as was the case for 0.05% peracetic acid after 1 min and 2 min. No sufficient efficacy against DHBV was seen when peracetic acid was used at concentrations of 0.001% and 0.005% after 1 min and 2 min as well as for 0.01% after 1-min exposure (RF between 1.36±0.35 and 3.55±0.60). These findings were different from those with VACV/MVA whose infectivity was already inactivated significantly by the use of 0.005% peracetic acid after 1 min. The hepatocytotoxicity of ethanol and isopropanol at the concentrations tested was <2.0 log10 TCID50 of DHBV whereas the hepatocytotoxicity of peracetic acid ranged between <2.0 (at concentrations of 0.001 – 0.005%) and <3.0 log10 TCID50 (at concentrations of 0.01 – 0.05%).
Glutardialdehyde showed a sufficient efficacy against DHBV corresponding to ≥4 log10 reduction in viral titers when it was used at concentration of 0.05% for ≥2 min and at concentration of 0.1% for ≥0.5 min. By contrast, 0.5-min exposure of 0.05% glutardialdehyde resulted in RF of 2.79±0.78. These results were in absolute concordance to VACV/MVA even though ≥4 log10 titer reduction could not always be achieved because of the high cytotoxicity of this biocide. This also applies to 0.7% formaldehyde which did not inactivate the infectivity of DHBV and VACV/MVA within 0.5-min exposure, but resulted most likely in sufficient activity after at least 30-min exposure. At the concentrations tested, the hepatocytotoxicity of glutardialdehyde was <3.0 and of formaldehyde <4.0 log10 TCID50 of DHBV.
In principle, enveloped viruses are considered to be relatively sensitive to biocides. However, validation of the virucidal action of disinfectants against HBV is essential since the hepatitis B virucidal activity of biocides cannot be predicted and the human blood plasma protects the virus from inactivation [22]. In particular, Payan et al. [13,23] have described HBV as an enveloped virus that may be less easy to inactivate. In Germany, a national test method has been published for the determination of limited virucidal activity [5] defined as active against enveloped viruses such as HBV, human immunodeficiency virus and hepatitis C virus (HCV) [24]. Unfortunately, feasible HBV infectivity assays have not yet been established for testing virucidal activity of biocides. Therefore, surrogate infectivity assays based on DHBV have been recommended to make a label claim of product efficacy to hepadnaviruses by the Environmental Protection Agency in the United States [25] and in Australia [26,27]. However, the DHBV model has also several disadvantages that prevent its use in virological laboratories. The model established in Germany [18] requires hepatocytes from DHBV-negative duck embryos for in vitro infection. By contrast, the virus has to be obtained from serum of ducks infected congenitally or experimentally. This is an unreliable source and no reasonable basis for standardization. Furthermore, the DHBV as all hepadnaviruses is non-cytopathogenic and additional methods such as the indirect immunofluorescent antigen staining are required to verify replication of DHBV in inoculated hepatocytes. That is why information is necessary if testing of biocides against DHBV is essential and should be included in several guidelines or whether disinfectants with the label claim limited virucidal activity according to the German DVV/RKI guideline can be regarded as effective against DHBV.
The present study compared the limited virucidal activity of five different biocides, which are often used as ingredients for commercially available disinfectants, against DHBV and VACV/MVA. The latter represent the classical test virus for the determination of limited virucidal activity. The data for VACV/MVA have been published recently to replace VACV by MVA because of the potential risk of laboratory-acquired infections caused by VACV [6]. To ensure the comparability of all data, the test samples used in both studies were generated by the same provider and the quantitative suspension tests were carried out most widely in accordance with the DVV/RKI guideline [5]. The concentrations of the biocides and the exposure times were chosen to see a kinetic of virus inactivation and a shift from non-effective to effective concentrations and exposure times. All tests were performed with a protein load of 10% FCS. Sufficient activity was shown by a virus titer reduction of at least 4 log10 with the exception of formaldehyde that resulted in highest cytotoxicity. In most studies reported in the literature, a compound was considered effective against DHBV when it reduced the viral titer by at 3 log10 units [26,28]. As the results of this study show, all basic biocides such as alcohols, peracetic acid, glutardialdehyde and formaldehyde inactivated DHBV and VACV/MVA at relatively low concentrations and within short exposure times. Against ethanol and isopropanol, a marginally higher susceptibility of DHBV could be detected. By contrast, the DHBV was more stable against peracetic acid than VACV/MVA. Gutardialdehyde and formaldehyde showed a comparable virucidal activity against both the DHBV and VACV/MVA. Limited unpublished results with HBV and the use of Tupaia cells correspond widely with these findings (personal communication: D. Glebe, Institute of Medical Virology, National Reference Centre for Hepatitis B and D, Justus Liebig University, Giessen, Germany). Thus, the data presented for DHBV are most likely also valid for HBV.
In addition to VACV/MVA, the BVDV has been recommended as second test virus for the determination of limited virucidal activity of disinfectants according to the German DVV/RKI guideline. Although the great susceptibility of BVDV, the surrogate of HCV, against biocides has not initiated extended studies, preliminary data suggest that this enveloped virus has a comparable if not a lower stability than VACV to biocides [29,30]. Comparable to the data presented in this study, the suitability of VACV and BVDV for determining activities of alcohol-based hand rubs against clinically relevant enveloped human viruses such as herpes simplex virus type 1 and type 2 as well as human and avian influenza A viruses has been reported in the literature [31].
Biocides which are effective against VACV/MVA, the most important model viruses for the determination of limited virucidal activity, show a reliable inactivation of DHBV infectivity. While peracetic acid is less effective against DHBV, alcohols are less effective against VACV/MVA. However, the findings show that DHBV and VACV/MVA exhibit a comparable tenacity. This means that the validation of disinfectants against DHBV can be omitted in practice when VACV or MVA have been included. It can be expected that in absence of more direct tests the results may be extrapolated to HBV.
The authors declare that they have neither financial nor non-financial competing interests.
AS: author of the publication, also provided analysis and interpretation of data, responsible for study design. MS: co-author of the publication, carried out the preparation of primary duck embryonic hepatocytes. BG: co-author of the publication, carried out the harvest of liver tissues from duck embryos. UB, HR and PW: co-authors of the publication and responsible for study design. All authors have read and approved the final manuscript.
The study was supported by the German Association for the Control of Virus Diseases e.V. (DVV).
The pre-publication history for this paper can be accessed here:
The authors are grateful to Christa Rothmann for her excellent technical assistance.
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's Canada Institute for Scientific and Technical Information in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


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I have missed my Lucky Strike, was dumb of me to get rid of it.
Got that Sun Spider, but just didn't like the kick-back hub so
I traded it to get my Airborne back.........
All is right with the World
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Hi guys,
I'm using a form to get the date of birth of a client. If they are older than 30, then they cannot submit the form. If they attempt to submit the form, they need to get the response error message saying 'You have to be younger than 30!'.
At the moment, the response message they get is 'Please fill in the required field.'
How do I change this to say 'You have to be younger than 30!'?
I have edited the response message of the 'date is earlier than minimum limit' to say the above message.
Here's what my form looks like:
First Name: [text* your-first-name]
Last Name: [text* your-last-name]
Date Of Birth: [date* date-634 min:1983-01-01 "You have to be younger than 30!"]
Please email my YEhangout invite to: [email* your-email]
I also have Twitter- my username is: [text text-225 id:Twitter]

[submit "Send"]


text:
MADRID — Spain insisted Wednesday it will continue to push for European financial aid to be delivered directly to its troubled banks, rather than count as government debt, warning that these were crucial moments for the euro currency union.
The Spanish government has agreed to seek a rescue package from its eurozone partners of up to €100 billion ($125 billion) for banks loaded with toxic assets after the collapse of a real estate bubble in 2008.
But under current rules, the money has to go through the government. The fear has grown that the government may be ultimately left to repay many of the banks' rescue loans and need a bailout of its own. As a result, investors are now demanding high interest rates to lend Spain money.
"I will continue to try to get direct recapitalization for the banks," Prime Minister Mariano Rajoy told Parliament.
It is not clear if a decision on the issue will be taken at the European Union summit in Brussels on Thursday and Friday.
Economy Minister Luis de Guindos told Parliament said he had held talks with eurozone colleagues Wednesday ahead of the summit.
"We face decisive hours in terms of the situation in the eurozone and the economic measures to be taken in Spain," he said
The interest rate for Spain's benchmark 10-year bonds — an indicator of investor wariness — was at 6.79 percent Wednesday, a rate considered unsustainable over the long term.
"The most urgent issue is financing," said Rajoy. "We can't continue for a long time to finance ourselves with these prices; there are many institutions and financial entities that don't have access to financial markets."
The Bank of Spain on Wednesday said the recession-stricken economy had slumped further in the second quarter and would likely post a sharper contraction than the 0.3 percent of the first three months.
The bank's June report said drops in consumer demand, car sales and industrial production "indicate activity has continued to diminish at a greater rhythm." Unemployment is at a staggering 24.4 percent.
Official second-quarter GDP figures are due to be published in July.


text:
QA Technician-Allison's Gourmet Kitchens Moore, OK
Reser's Fine Foods - Moore, OK
This job posting is no longer available on Reser's Fine Foods. Find similar jobs:Reser's Fine Foods jobs
About this company
Reser's Fine Foods makes a variety of food items that have shown up at barbeques and other gatherings for decades. The company offers...


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New Litter of English Setters - English Setters in Hudson, Wisconsin - Hudson, WI Setters
Eight 10 day old puppies. Six female and two male. Dam - Annie Oakley (White and Orange) / Sire - Roy Rogers (tri-color). Dam and Sire are both registered pure bred from Berg Brothers and fantastic gun dogs.
Ad location: Hudson, Wisconsin
Ad created: Mar 01, 2014
Contact Information
Contact Name: Greg Miller
715-381-7387Contact breeder through email
Hudson, WI Setters Breeder Information
My Classified Pictures
Billy the Kid - One of two males. Tricolor
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Miss Dolly - one of three tricolor females


text:
Illuminate any dark spaces in style with this set of three lamps. Included in the collection are two table lamps and a tall floor light. Each piece features a dark metal base and bell-shaped, traditional, light linen shades. The classic look, clean lines and streamlined design of each of these lamps will coordinate beautifully with virtually every design theme and color palette. Curl up with your favorite book under the light of these lamps.
Floor lamp is 59" high
Number of Pieces in Set: 3
Includes: 1 Floor Lamp, 2 Table Lamps
Body Material: Metal
Shade Material: Linen
Metal Finish: Bronze
Switch Type: Rotary
Maximum Light Bulb Wattage: 150 Watt Incandescent
Table Lamp Dimensions: 29.5 " H x 6.0 " W x 6.0 " D
Table Top Shade Dimensions: 10.0 " H
Table Bottom Shade Dimensions: 14.0 " W
Floor Lamp Dimensions: 60.5 " H x 10.0 " W x 10.0 " D
Floor Top Shade Dimesnsions: 10.0 " H
Floor Bottom Shade Dimensions: 14.0 " W
Accent Lamp Dimensions: 29.5 " H x 6.0 " W x 6.0 " D
Accent Shade Top Dimensions: 10.0 " H
Accent Shade Bottom Dimensions: 14.0 " W
Shade Warning: Don't use with shade less than 4.5" diameter top ring. Bottom rng shuld be equal to or larger than top ring
Assembly Details: assembly required, tools not provided
Lamp Type: Floor, Table, Table
Quantity: 1, 1, 1
California Title 20 Compliance: CA Title 20 compliant
other Info.
- Online Item #: 12770565
- Store Item Number (DPCI): 074-14-2926
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text:
Out of 80 million Egyptians, maybe 300,000 to 500,000 participated in the demonstrations. So what if not all Palestinians are Hamas? You don't need that many to change history, just like in Egypt. The biggest problem with Palestinian society and their supports is that they keep telling us that moderates exist among them. But where are they and when are they going to take on the radicals and bring them down? Because if they don't do this, then the radicals will dictate events and history, as they are doing now. To make a s statement that not all Palestinians are Hamas is to demonstrate that you have no idea how and who impacts history.
U.S. and Russia discuss flight safety in Syria on conference call (Reuters)
from the article: Israel must remember: Not all Palestinians are Hamas