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Olfactory receptors are key components in signal transduction. Mutations in olfactory receptors alter the odor response, which is a fundamental response of organisms to their immediate environment. Understanding the relationship between odorant response and mutations in olfactory receptors is an important problem in bioinformatics and computational biology. In this work, we have systematically analyzed the relationship between various physical, chemical, energetic and conformational properties of amino acid residues, and the change of odor response/compound's potency/half maximal effective concentration (EC50) due to amino acid substitutions.
We observed that both the characteristics of odorant molecule (ligand) and amino acid properties are important for odor response and EC50. Additional information on neighboring and surrounding residues of the mutants enhanced the correlation between amino acid properties and EC50. Further, amino acid properties have been combined systematically using multiple regression techniques and we obtained a correlation of 0.90-0.98 with odor response/EC50 of goldfish, mouse and human olfactory receptors. In addition, we have utilized machine learning methods to discriminate the mutants, which enhance or reduce EC50 values upon mutation and we obtained an accuracy of 93% and 79% for self-consistency and jack-knife tests, respectively.
Our analysis provides deep insights for understanding the odor response of olfactory receptor mutants and the present method could be used for identifying the mutants with enhanced specificity.
Membrane proteins perform several functions, including the transport of ions and molecules across the membrane, binding to small molecules at the extracellular space, recognizing the immune system and energy transducers. Olfactory receptors (OR) are membrane proteins, belonging to the G Protein-Coupled Receptor superfamily, which are characterized by the presence of hydrophobic transmembrane domains. The odorant response of an organism by ORs to its environment forms the basis for our understanding in intra-species interactions, host-pathogen interactions, balance of chemicals, cell-cell interactions and other fundamental processes. It is evident that individual odorant can be recognized by multiple ORs and conversely, one type of OR can recognize multiple odorants with distinct binding affinities and specificities [1,2]. The binding and response of ORs with odorants are critical for the conversion of chemical information into electronic signals in olfactory sensory neurons [3,4]. Recent studies showed that mosquitoes' odorant receptors help the insects to find humans and, inadvertently, to transmit malaria [5,6]. Further, ORs have been analyzed to understand the mechanism of chloride uptake [7], modulation of signaling [8], functional architecture of olfactory system [9], unitary response [10], structural and functional plasticity at binding pocket [11] etc. Similar analysis has also been reported for identifying the binding site residues and binding specificity of protein-protein complexes [12-17].
The importance of specific amino acid residues in ORs and other membrane proteins has been demonstrated through site-directed mutagenesis experiments. The experimental data on EC50, maximal velocity of transport, odorant response, percentage uptake of compounds, affinity and specificity have been accumulated in the database for functional residues in membrane proteins [18]. Kuang et al. [19] measured the EC50 values for lysine in the wild type and mutants of 5.24 receptor. Luu et al. [20] elucidated the features of olfactory receptors for determining ligand specificity using different amino acid agonists. The structural basis for mouse OR to EC50 data has been analyzed by systematically substituting amino acid residues in different transmembrane helical segments [2,21]. Schmiedeberg et al. [22] carried out docking studies to understand the influence of different chemical compounds as well as due to mutations. On the other hand, computational methods have been proposed to understand the binding affinity of ligands with ORs using the template structure of rhodopsin [23-25].
In spite of these studies, the role of amino acid properties for the change of EC50 or odorant response has not yet been explored. Further, it is necessary to develop computational models to discriminate the mutants, which increase or decrease EC50. In this work, we have constructed different datasets of goldfish, mouse and human ORs for the mutants that change the odorant response, increase cAMP (adenosine 3'-5'-cyclic mono phosphate) and EC50 values. The differences in experimental data (EC50/odor response etc.) upon mutations have been related with physical, chemical, energetic and conformational properties of amino acid residues and the important amino acid properties have been brought out. The combinations of amino acid properties and the influence of neighboring and surrounding residues have been successfully used to relate the experimental functional data. Further, machine learning methods have been utilized to discriminate mutants with enhanced EC50 values.
Materials and methods
We have developed a database, TMFunction, for functionally important amino acid residues in membrane proteins [18]. TMFunction has been searched for all functional data available for ORs. We obtained the experimental data, EC50, odorant response and cAMP increase for goldfish, mouse and human ORs. The final dataset contains 119 data with the following categories: (i) EC50: goldfish OR with Lys, 12; Arg : 12; Gly: 6 and Glu: 6; mouse OR: 28; Human OR: 7; (ii) odorant response: cAMP increase: 24 and Ca2+ increase: 24.
Amino acid properties
We used a set of 49 diverse amino acid properties (physical, chemical, energetic and conformational), which fall into various clusters analyzed by Tommi and Kanehisa [26] in the present study. The amino acid properties were normalized between 0 and 1 using the expression:
where P(i), Pnorm(i) are, respectively, the original and normalized values of amino acid i for a particular property, and Pmin and Pmax are, respectively, the minimum and maximum values. Further, the numerical and normalized values for all the 49 properties used in this study along with their brief descriptions have been explained in our earlier articles [27,28] and are available on the web at http://www.cbrc.jp/~gromiha/fold_rate/property.html webcite. These properties have been successfully used to understand the folding and stability of proteins [29-33].
Computational procedure
The mutation induced changes in property values ΔP(i) was computed using the equation [29]:
where Pmut(i) - Pwild(i) are, respectively, the property value of the ith mutant and wild type residues, and i varies from 1 to N; total number of mutants. The computed difference in property values ΔP (X in Eqn. 3) was related with experimental EC50 or odorant response (Y in Eqn. 3) using single correlation coefficient (r). It is given by:
Molecular modelling of mouse OR73
Mouse olfactory receptor 73 (OR73) sequence was obtained from NCBI (http://www.ncbi.nlm.nih.gov/guide/ webcite) using text search. The TM regions and topology (N-terminus OUT/IN) of the sequence were predicted using the transmembrane prediction server HMMTOP [34]. The sequences of OR73 and the template (bovine rhodopsin; PDB ID: 1F88A) were aligned using PRALINE-TM [35] server and manually edited using JALVIEW [36] Version 2.4. The TM region predictions from HMMTOP and the two conserved motifs MAYDRYVAIC and NPXXY in OR73 were used to guide and improve the alignment of the query and the template.
The crystal structure of the bovine rhodopsin [23] (PDB ID: 1f88 chain A) was used as a template for the comparative modelling of the query (OR73). The structure of the template was obtained from RCSB (http://www.rcsb.org/pdb webcite). The coordinates corresponding to the residues 236-239 and 328-333 were not available in the crystal structure of 1F88 chain A due to poor electron density and hence these residues were removed from the template sequence before the alignment. The final alignment (Figure 1) was used to construct the model using the software MODELLER [37] (version 9.8). A set of 20 structures were generated from which the five least probability density function models were validated by using PROCHECK [38] (Ramachandran Plot). The best structure was further energy minimized using the SYBYL software package (version 7.2) (Tripos Associates Inc.). Tripos force field, using 100 iterations of Powell's gradient with a distance dependent dielectric constant of 1 and a non bonded interaction cut off value of 8 and was terminated at a convergence of 0.05kcal mol. The final structures were further validated using PROCHECK [38].
Figure 1. Alignment of query (OR73) with template (1F88). Observed and predicted TM helix positions are marked in VIGBYOR representation. Key functional motifs are shown in pink color against OR73 sequence.
Local sequence and structural effects
The effect of local sequence, Pseq(i), was included using the equation [29]:
where, Pmut(i) is the property value of the ith mutant residue and ΣPj(i) is the total property value of the segment of (2k+1) residues ranging from i-k to i+k about the ith residue of wild type.
The structural information, Pstr(i), was included using the equation:
where Pmut(i) is the property value of the ith mutant residue, and:
where, nij is the total number of type j residues surrounding the ith residue of the protein within the volume of 8Å, and Pj is the property value of residue type j. Further details about the computation of surrounding residues have been described in our earlier articles [39,40].
Multiple regression analysis
We have combined the amino acid properties using multiple regression technique: multiple correlation coefficients and regression equations were determined using standard procedures [41]. When fitting the data by multiple regression technique, reducing the number of variables increases the reliability of results. Hence, we selected three to five properties by searching all possible combinations of the 49 properties and computed the multiple correlation coefficients for all data sets. The highest correlation coefficient was selected and used in the analysis.
Machine learning methods
We have analyzed several machine learning techniques implemented in WEKA program [42] for discriminating mutants with enhanced EC50 values. This program includes several methods based on Bayes function, Neural network, Radial basis function network, Logistic function, Support vector machine, Regression analysis, Nearest neighbor, Meta learning, Decision tree and Rules. The details of all these methods are available in our earlier articles [43-45].
Jack-knife test
We have performed a jack-knife (leave-one-out cross-validation) test for assessing the validity of the present work. In this method, n-1 data are used for training and the left out mutant is used for testing.
Assessment of predictive ability
We have used different measures, such as sensitivity, specificity and accuracy to assess the performance of discriminating mutants with enhanced function. These terms are defined as follows:
where, TP, FP, TN and FN refer to the number of true positives, false positives, true negatives and false negatives, respectively.
Results and discussion
Relationship between amino acid properties and change in EC50 upon mutation: goldfish OR with Lys potency
We have computed the changes in amino acid properties using Eqn.2 and related them with changes in EC50 upon mutations using Eqn. 3. The accessible surface area of unfolding showed a negative correlation of -0.86 for Lys potency with goldfish OR, and other physical properties, bulkiness, volume etc. showed appreciable negative correlation with ΔEC50 [46]. Figure 2a shows the relationship between ΔASA and ΔEC50. On the other hand, entropy change has high positive correlation with ΔEC50. Figure 2b shows the trend between -TΔS and ΔEC50 and the correlation coefficient is 0.85.
Figure 2. (a) Relationship between ΔASA and ΔEC50. (b) Variation of -TΔS with ΔEC50. (c) Variation of correlation coefficient with number of properties.
We have analyzed the combined effect of different amino acid properties and related with ΔEC50 values. The variation of correlation coefficient with number of properties is shown in Figure 2c. We noticed that the combination of four properties raised the correlation up to 0.988.
Arg potency
The data on Arg potency with goldfish OR showed a maximum correlation of 0.62 with the property, reduction in accessibility. Further, the combination of five properties also showed the maximum correlation of 0.90. Hence, we have included the information of neighboring residues along the sequence using Eqn. 4. We used different window lengths and the results for window lengths 3, 5 and 7 for the combination of 2, 3, and 4 amino acid properties is shown in Figure 3a.
Figure 3. (a) Variation of correlation coefficients with different window lengths and combination of amino acid properties: purple: 1 property; brown: 2 properties; yellow: 3 properties and blue: 4 properties. (b) Relationship between experimental and predicted ΔEC50 values.
We observed that the combination of four properties raised the correlation up to 0.98. This result shows that the experimental EC50 values are not depending on a specific residue and the information on neighboring residues are very important for the variation of EC50 upon mutation. The experimental and observed ΔEC50 values for all the 12 mutants are shown in Figure 3b and we noticed a good relationship between them. Further, the thermodynamic properties, ΔG, ΔH and -TΔS showed good correlation with ΔEC50 for goldfish OR with Glu and Gly potency.
Molecular modeling and structural analysis of mouse OR
A three-dimensional model of the mouse olfactory receptor, OR73, was obtained as mentioned in Methods. PROCHECK results for the model, excluding the loop regions, show more than 95% of residues in allowed regions (including strictly allowed and partially allowed) of the Ramachandran plot. The full-length structures of the models show more than 90% in the allowed regions (including strictly allowed and partially allowed) of the Ramachandran plot. The residues that were found in the disallowed regions were mainly in the loop regions which are highly variable in length and sequence identity. The RMSD of the full length OR model with respect to template was found to be less than 2Å .The amino acid mutations under study were marked on the model as shown in Figure 4. Many of the mutants lie in TM5 and TM6 and the loop in between has a glycine residue and this region could be implicated in the dimer interface. Most of the other 24 mutants are in intracellular loops.
Figure 4. Homology model of mouse OR73 (7 TM helices-VIBGYOR, Mutations-Black).
The solvent accessibility of the amino acid residues, chosen for this study, were observed in the three-dimensional model. These values were calculated using PSA [47] within JOY version 3.2 [48,49] (Additional file 1). It is interesting that only 7 out of 24 mutants are solvent-buried and three of them are in TM3. Such solvent-buried residues could be important for the structural integrity of a protomer.
Additional file 1. Solvent accessibility of amino acids under study.
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Relationship between amino acid properties and change in EC50/cAMP increase/Ca2+ increase upon mutation: mouse OR
Katada et al. [21] measured the EC50 values for the mutants at various positions in the transmembrane helices of mouse OR. Figure 4 shows a model for mouse OR and the information about mutated residues.
We have computed the difference in amino acid properties and related with difference in EC50 values. We observed a maximum correlation of just 0.38 and the combination of five properties raised the correlation only up to 0.56. We have included the information on neighboring residues, which increased the correlation up to 0.76. We then tried to utilize the structural information of mouse OR using Eqn. 5. The combination of mutants, neighboring residues and surrounding residues enhanced the correlation up to 0.81.
cAMP level increase
Kato et al. [2] measured the increase in cAMP level and Ca2+ for 24 mutants, which are located in transmembrane helical and loop regions. The analysis with overall data did not show high correlation and hence we classified the mutants based on their locations. Interestingly, the classification improved the correlation for the mutants both in transmembrane and loop regions.
We obtained the correlation of 0.86 with the combination of three properties for the mutants in membrane regions. In the loop region, a single property, -TΔS showed a correlation of -0.89 using the information of two neighboring residues on both sides of the mutants (Figure 5a). The combination of three properties raised the correlation up to 0.98. Figure 5b shows the relationship between the experimental and predicted cAMP level increase.
Figure 5. (a) Relationship between -TΔS and cAMP increase in the loop region of mouse OR. (b) Comparison of experimental and predicted cAMP increase levels with the combination of three properties.
We have analyzed the variation of Ca2+ increase upon mutations and we observed that the classification of mutants based on their location is necessary for understanding the properties influencing Ca2+ increase. The results obtained for transmembrane helical and loop regions are shown in Figures 6a and 6b, respectively. We obtained a maximum single correlation of 0.90 with the propensity of residues in the middle of α-helix for the mutants in transmembrane helices (Figure 6a). The relationship between experimental and predicted Ca2+ increase for the mutants in the loop regions using a combination of three properties is shown in Figure 6b. The correlation coefficient is 0.995.
Figure 6. (a) Relationship between αm and Ca2+ increase for the mutants in membrane spanning helical segments. (b) Comparison of experimental and predicted Ca2+ increase for the mutants in loop regions.
Relationship between amino acid properties and change in EC50 upon mutation: human OR
Schmiedeberg et al. (2007) measured the EC50 values for seven mutants in human OR. We found that bulkiness has a correlation of 0.71 and the information on three neighboring residues increased the correlation up to 0.92 (Figures 7a and 7b). This analysis reveals the importance of neighboring residues for determining the change in EC50. Further, the combination of just two properties increased the correlation up to 0.999 and Figure 7c showed the relationship between experimental and predicted ΔEC50.
Figure 7. Relationship between bulkiness and ΔEC50 in human OR; (a) Mutant information and (b) Three neighboring residues (c) Comparison of experimental and predicted ΔEC50.
Discrimination of mutants that enhanced/decreased EC50: mouse OR
We have collected a set of 28 data in mouse OR in which 15 of them increased EC50 upon mutation and 13 mutants decreased the EC50 values. We made an attempt to discriminate these mutants using the information on wild type residue, mutant residue and the properties of neighboring residues. We have utilized several machine learning techniques for discrimination. Our method showed an accuracy of 92.9% for self-consistency test and the sensitivity and specificity are 93.3% and 92.3%, respectively. The assessment using jack-knife test showed an accuracy of 78.6% and the sensitivity and specificity are 80.0% and 76.9%, respectively. This method can be used to identify the mutants with increased/decreased EC50.
We have constructed different datasets for mouse, goldfish and human ORs and various experimental data such as EC50, odorant response, Ca2+ increase etc. The experimental data have been systematically analyzed with physical, chemical, energetic and conformational properties of amino acid residues and important properties have been listed out. We found that the information on neighboring and surrounding residues, namely the inclusion of motifs, is important to understand the function. Further, we have combined the amino acid properties using multiple regression analysis, which relates experimental EC50/cAMP level increase and Ca2+ increase very well. We have utilized machine learning techniques for discriminating the mutants that enhance/reduce IC50 upon mutation and we obtained an accuracy of 93% and 79%, respectively, for self-consistency and jack-knife tests. The results obtained in the present work would help to understand the importance of amino acid properties to the functions of ORs and to identify the mutants with enhanced EC50 values.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
MMG, RS and KF conceived the project. MMG carried out the computations and wrote the manuscript. KH modeled the structures of ORs and analyzed them. MMG, KH, RS and FK contributed in discussions. KH and RS wrote the sections about modeling. All authors read and finalized the manuscript.
This research was partially supported by Indian Institute of Technology Madras research grant (BIO/10-11/540/NFSC/MICH), Indo-Japan Grant of Department of Biotechnology, India and National Institute of Advanced Industrial Science and Technology, Japan. We would like to thank IIT Madras, Computational Biology Research Center and NCBS (TIFR) for infrastructural facilities.
This article has been published as part of BMC Bioinformatics Volume 13 Supplement 7, 2012: Advanced intelligent computing theories and their applications in bioinformatics. Proceedings of the 2011 International Conference on Intelligent Computing (ICIC 2011). The full contents of the supplement are available online at http://www.biomedcentral.com/bmcbioinformatics/supplements/13/S7.
Zhao H, Ivic L, Otaki JM, Hashimoto M, Mikoshiba K, Firestein S: Functional expression of a mammalian odorant receptor.
Science 1998, 279:237-242. PubMed Abstract | Publisher Full Text
Kato A, Katada S, Touhara K: Amino acids involved in conformational dynamics and G protein coupling of an odorant receptor: targeting gain-of-function mutation.
J Neurochem 2008, 107:1261-1270. PubMed Abstract | Publisher Full Text
Buck LB: Information coding in the vertebrate olfactory system.
Annu Rev Neurosci 1996, 19:517-544. PubMed Abstract | Publisher Full Text
Mombaerts P: Genes and ligands for odorant, vomeronasal and taste receptors.
Nat Rev Neurosci 2004, 5:263-278. PubMed Abstract | Publisher Full Text
Leal WS: Behavioural neurobiology: The treacherous scent of a human.
Nature 2010, 464:37-38. PubMed Abstract | Publisher Full Text
Carey AF, Wang G, Su CY, Zwiebel LJ, Carlson JR: Odorant reception in the malaria mosquito Anopheles gambiae.
Nature 2010, 464:66-71. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Jaén C, Ozdener MH, Reisert J: Mechanisms of chloride uptake in frog olfactory receptor neurons.
J Comp Physiol A Neuroethol Sens Neural Behav Physiol 2011, 197:339-349. PubMed Abstract | Publisher Full Text
Hall RA: Autonomic modulation of olfactory signaling.
Sci Signal 2011, 4:pe1. PubMed Abstract | Publisher Full Text
Abuin L, Bargeton B, Ulbrich MH, Isacoff EY, Kellenberger S, Benton R: Functional architecture of olfactory ionotropic glutamate receptors.
Neuron 2011, 69:44-60. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Ben-Chaim Y, Cheng MM, Yau KW: Unitary response of mouse olfactory receptor neurons.
Proc Natl Acad Sci USA 2011, 108:822-827. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Baud O, Etter S, Spreafico M, Bordoli L, Schwede T, Vogel H, Pick H: The mouse eugenol odorant receptor: structural and functional plasticity of a broadly tuned odorant binding pocket.
Biochemistry 2011, 50:843-853. PubMed Abstract | Publisher Full Text
Ezkurdia L, Bartoli L, Fariselli P, Casadio R, Valencia A, Tress ML: Progress and challenges in predicting protein-protein interaction sites.
Brief Bioinform 2009, 10:233-246. PubMed Abstract | Publisher Full Text
Yanay O, Rost B: Protein-protein interaction hotspots carved into sequences.
PLoS Comput Biol 2007, 3:e119. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
You ZH, Lei YK, Huang DS, Zhou X: Using manifold embedding for assessing and predicting protein interactions from high-throughput experimental data.
Bioinformatics 2010, 26(21):2744-2751. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Xia JF, Zhao XM, Song J, Huang DS: APIS: accurate prediction of hot spots in protein interfaces by combining protrusion index with solvent accessibility.
BMC Bioinformatics 2010, 11:174. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text
Xia JF, Zhao XM, Huang DS: Predicting protein-protein interactions from protein sequences using meta predictor.
Amino Acids 2010, 39:1595-1599. PubMed Abstract | Publisher Full Text
Gromiha MM, Yokota K, Fukui K: Energy based approach for understanding the recognition mechanism in protein-protein complexes.
Mol Biosyst 2009, 5(12):1779-1786. PubMed Abstract | Publisher Full Text
Gromiha MM, Yabuki Y, Suresh MX, Thangakani AM, Suwa M, Fukui K: TMFunction: database for functional residues in membrane proteins.
Nucleic Acids Res 2009, 37:D201-D204. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Kuang D, Yao Y, Wang M, Pattabiraman N, Kotra LP, Hampson DR: Molecular similarities in the ligand binding pockets of an odorant receptor and the metabotropic glutamate receptors.
J Biol Chem 2003, 278:42551-42559. PubMed Abstract | Publisher Full Text
Luu P, Acher F, Bertrand HO, Fan J, Ngai J: Molecular determinants of ligand selectivity in a vertebrate odorant receptor.
J Neurosci 2004, 24:10128-10137. PubMed Abstract | Publisher Full Text
Katada S, Hirokawa T, Oka Y, Suwa M, Touhara K: Structural basis for a broad but selective ligand spectrum of a mouse olfactory receptor: mapping the odorant-binding site.
J Neurosci 2005, 25:1806-1815. PubMed Abstract | Publisher Full Text
Schmiedeberg K, Shirokova E, Weber HP, Schilling B, Meyerhof W, Krautwurst D: Structural determinants of odorant recognition by the human olfactory receptors OR1A1 and OR1A2.
J Struct Biol 2007, 159:400-412. PubMed Abstract | Publisher Full Text
Palczewski K, Kumasaka T, Hori T, Behnke CA, Motoshima H, Fox BA, Le Trong I, Teller DC, Okada T, Stenkamp RE, Yamamoto M, Miyano M: Crystal structure of rhodopsin: a G protein-coupled receptor.
Science 2000, 289:739-745. PubMed Abstract | Publisher Full Text
Man O, Gilad Y, Lancet D: Prediction of the odorant binding site of olfactory receptor proteins by human-mouse comparisons.
Protein Sci 2004, 13:240-254. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Olender T, Feldmesser E, Atarot T, Eisenstein M, Lancet D: The olfactory receptor universe - from whole genome analysis to structure and evolution.
Genet Mol Res 2004, 3:545-553. PubMed Abstract | Publisher Full Text
Tomii K, Kanehisa M: Analysis of amino acid indices and mutation matrices for sequence comparison and structure prediction of proteins.
Protein Eng 1996, 9:27-36. PubMed Abstract | Publisher Full Text
Gromiha MM, Oobatake M, Sarai A: Important amino acid properties for enhanced thermostability from mesophilic to thermophilic proteins.
Biophys Chem 1999, 82:51-67. PubMed Abstract | Publisher Full Text
Gromiha MM, Oobatake M, Kono H, Uedaira H, Sarai A: Importance of surrounding residues for protein stability of partially buried mutations.
J Biomol Struct Dyn 2000, 18:281-295. PubMed Abstract | Publisher Full Text
Gromiha MM, Oobatake M, Kono H, Uedaira H, Sarai A: Role of structural and sequence information in the prediction of protein stability changes: comparison between buried and partially buried mutations.
Protein Eng 1999, 12:549-555. PubMed Abstract | Publisher Full Text
Gromiha MM, Selvaraj S: Important amino acid properties for determining the transition state structures of two-state protein mutants.
FEBS Lett 2002, 526:129-134. PubMed Abstract | Publisher Full Text
Huang LT, Gromiha MM: Reliable prediction of protein thermostability change upon double mutation from amino acid sequence.
Bioinformatics 2009, 25:2181-2187. PubMed Abstract | Publisher Full Text
Huang LT, Gromiha MM: First insight into the prediction of protein folding rate change upon point mutation.
Bioinformatics 2010, 26:2121-2127. PubMed Abstract | Publisher Full Text
Ou YY, Chen SA, Gromiha MM: Classification of transporters using efficient radial basis function networks with position-specific scoring matrices and biochemical properties.
Proteins 2010, 78:1789-1797. PubMed Abstract | Publisher Full Text
Tusndy GE, Simon I: The HMMTOP transmembrane topology prediction server.
Bioinformatics 2001, 17:849-850. PubMed Abstract | Publisher Full Text
Pirovano W, Feenstra KA, Heringa J: PRALINETM: a strategy for improved multiple alignment of transmembrane proteins.
Bioinformatics 2008, 24(2):492-497. PubMed Abstract | Publisher Full Text
Waterhouse AM, Procter JB, Martin DMA, Clamp M, Barton GJ: Jalview version 2--a multiple sequence alignment editor and analysis workbench.
Bioinformatics 2009, 25:1189-1191.
doi: 10.1093/bioinformatics/btp033PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Sali A, Blundell TL: Comparative protein modelling by satisfaction of spatial restraints.
J Mol Biol 1993, 234:779-815. PubMed Abstract | Publisher Full Text
Laskowski RA, MacArthur MW, Moss DS, Thornton JM: PROCHECK: a program to check the stereochemical quality of protein structures.
J Appl Crystallogr 1993, 26:283-291. Publisher Full Text
Gromiha MM, Selvaraj S: Inter-residue interactions in protein folding and stability.
Prog Biophys Mol Biol 2004, 86:235-277. PubMed Abstract | Publisher Full Text
Gromiha MM: Influence of long-range contacts and surrounding residues on the transition state structures of proteins.
Anal Biochem 2011, 408:32-36. PubMed Abstract | Publisher Full Text
Grewal PS: Numerical Methods of Statistical Analysis. New Delhi: Sterling Publ; 1987.
Witten IH, Frank E: Data Mining: Practical Machine Learning Tools and Techniques. 2nd edition. San Francisco: Morgan Kaufmann; 2005.
Gromiha MM, Suwa M: Discrimination of outer membrane proteins using machine learning algorithms.
Proteins 2006, 63:1031-1037. PubMed Abstract | Publisher Full Text
Gromiha MM, Yabuki Y: Functional discrimination of membrane proteins using machine learning techniques.
BMC Bioinformatics 2008, 9:135. PubMed Abstract | BioMed Central Full Text | PubMed Central Full Text
Gromiha MM, Suwa M: Influence of amino acid properties for discriminating outer membrane proteins at better accuracy.
Biochim Biophys Acta 2006, 1764:1493-1497. PubMed Abstract | Publisher Full Text
Gromiha MM, Sowdhamini R, Fukui K: Structure-function relationship in olfactory receptors.
Lee B, Richards FM: The interpretation of protein structures: estimation of static accessibility.
J Mol Biol 1971, 55(3):379-400. PubMed Abstract | Publisher Full Text
Overington J, Johnson MS, Sali A, Blundell TL: Tertiary structural constraints on protein evolutionary diversity: templates, key residues and structure prediction.
Proc Biol Sci 1990, 241:132-145. PubMed Abstract | Publisher Full Text
Mizuguchi K, Deane CM, Blundell TL, Johnson MS, Overington JP: JOY: protein sequence-structure representation and analysis.
Bioinformatics 1998, 14:617-623. PubMed Abstract | Publisher Full Text


text:
Bloom is a fruity floral, like most scents for young women, and there's nothing artificial about the aroma. In fact, it smells a heck of a lot like one of my favorite scents, Hanae Mori Butterfly ($90), although a little less sweet and rich.
Bloom opens up with a soft burst of honeydew melon, followed by a round peony note that complements it nicely. Then, it settles down into a honeyed peony that wears close to the skin. It's not super complex, but it's distinctly pretty. And if you love Butterfly, it's a nice lower-priced alternative.

subset: commoncrawl


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Houston County Courier - Local News
Stories Added - August 2009
Copyright 2009 - Polk County Publishing Company
Joe Barton holds town hall meeting
Houston County Courier - August 2009
By Clyde Black
LOVELADY – A significant number of Houston County residents turned out for the town hall meeting hosted by Congressman Joe Barton at the Lovelady Old Gym on Monday afternoon, Aug. 17.
Approximately 300 people showed up for the hour-long meeting.
Congressman Barton addressed the Health Care Bill and then fielded questions from the audience.
The meeting was positive and most questions from the crowd were directed at government intervention, government spending and about the local desire to see more traditional representation.
For example, one citizen spoke to the issue of maintaining God on our currency and the 10 commandments on government buildings.
The crowd seemed to be centered behind that type of questions.
Polk County Publishing Company
Copyright 2009
Contact Us: email@example.com
Call us at - (936) 327-4357
Webmaster: Gregg Faith


text:
With Heart of the Swarm released it's time to roll out the purple network cable and get some LS SC2 LAN action!
Sunday's are always chilled compared to Saturday's at LS. By Sunday most of the kinks in the network/internet are sorted so it's mostly smooth sailing.
We saw nearly 30 SC2 players make the journy. Shout outs to Jessie Hill for taking the shoutcaster point man and bringing his sweet charm to the stream.
Thanks goes also to Gibstech for lending five of his awesome machines to be played on. Speaking of machines, LanSmash now has ten gaming rigs for use for tournaments. Gibbo mentioned to me that he's planning on building another five, so with combined strength that's twenty gaming rigs for use.
At its heart LanSmash will always remain a BYOC event. I personally think to reach out new gamers we need to provide at least some machines for gamers to play on. I think once they see the benefits of bringing your own gear (play more games, more familar setup) they'll start bringing their own gear in time.
Thanks to all that came! Look forward to seeing you all at the next event!


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Does anyone else consider this a completely acceptable lunch?
Quality Lunch
Arielle says
I'm a new follower of your blog and I absolutely love it!! I'm not super girly so your hair tutorials make it really easy for me to have cute hairstyles. Also, I LOVE this Sicilian lemonade. It's the best!
Sheri says
It looks YUMMY to me:)
Our Life's Blessings


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On-Campus: 94444
Off-Campus: 610-519-4444
610-519-6979 (option #1)
We are located at Farrell Hall
on Lancaster Ave.
In light of the tragic happenings at Virginia Tech on April 16, 2007, it is important that you understand the procedures Villanova University has in place and how we proceed and respond in the case of an emergency on campus.
In addition to day-to-day coverage by our 75-member Public Safety team, we have an extensive Emergency Preparedness Plan in place that includes procedures to ensure a rapid response to an unanticipated emergency or threatening situation. Villanova takes all incidents affecting student, faculty, and staff safety as serious and time critical.
Villanova University has devoted significant resources to providing a safe campus and assuring the safety of our community members. Our Public Safety department is responsible for campus security and consists of approximately 75 officers who provide on-campus coverage 24 hours a day, seven days a week. To assure the greatest breadth of coverage, the officers patrol the campus on foot, on bicycle, and in vehicles.
While our Public Safety officers are well-trained professionals, they do not have the authority to arrest anyone on campus. The department, however, maintains excellent relationships with both the Lower Merion and Radnor Police Departments, the latter of which has jurisdiction on campus to respond to criminal behavior and can provide immediate response when necessary.
We encourage members of the campus community or any visitor to immediately report suspicious activity or an emergency to the Public Safety department by calling 9-4444 from an on-campus telephone or 610-519-4444 from an outside telephone or cell phone. If an emergency requires the assistance of an outside agency, the Public Safety department will contact the appropriate organization as the situation dictates.
If a situation arises on or around campus that could potentially threaten the health or safety of University community members, senior officers of the University and members of Public Safety are immediately summoned as part of Villanova's crisis response procedure. This group determines the appropriate actions to be taken, using Villanova University's Emergency Preparedness Plan as its basis and guideline.
This action may include:
The University has the capability via the Wildcard system to control access to virtually all buildings and entrance gates on campus. This is an access control system, not a security system, but it can regulate what buildings and rooms cardholders may enter.
In the event of an emergency, an effective University-wide communications process is vital in order to provide the greatest safety possible for our students, faculty, and staff.
We will use all available means of communications in a crisis situation, including text messaging, telephone, the Web site, and e-mail. The University will also implement on-site personal contacts by utilizing Public Safety officers and other University staff and officials. The communications tools may include:
Villanova Web Site
NOVA Alert
Personal Interaction and Connection
Villanova routinely examines the Emergency Preparedness Plan and devises measures to respond to various incidents as quickly and effectively as possible. Father Peter M. Donohue, O.S.A. has asked the senior administration to review and assess the Emergency Preparedness Plan, as well as our communication strategies to assure we have accounted and prepared for as many scenarios as possible.
In the event of an emergency on campus or off, your well-being is critically important to us. We provide full-service counseling services, as well as access to Campus Ministry staff, who are trained to provide grief counseling. If you would like further information about the specific services we make available to our students, faculty, and staff, you may find the following links helpful.


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Feb. 23, 2011
MADISON, Wis. -- In preparation for this weekend's playoff match-up, UWBadgers.com caught up with sophomore Breann Frykas (East St. Paul, Manitoba) to get her take on the Badgers' series against St. Cloud State at the Eagles Nest in Verona, Wis.Q: What's the difference between competing playing in the playoffs compared to a regular-season series?Frykas: There is a lot more excitement. There's going to be a little bit of nervous butterflies but I think we can convert that into a lot of positive energy. It's like a new season; whatever we've done is done and we have to take the next step forward to complete our next goal and go to the WCHA finals.Q: Does the team prepare any differently for a playoff series?Frykas: I think we've been developing our strategies and our commitment to everything throughout the season and making good habits. We just want to keep doing what we've been doing and hopefully we'll be successful.Q: How confident do you think the team is going into the first round of the playoffs?Frykas: I think we're pretty confident. We have 30 wins under our belt, which is great. Everyone's contributed to those wins, and I think if we just keep doing what we're doing we'll be successful in the first round. We have to take one game at a time and one team at a time.Q: Does playing essentially at home help add to that confidence?Frykas: Yeah, definitely. Just having those fans up there and supporting you all the time really helps. It's nice to have your colors in the stands when you look up when you score a goal. It helps out a lot and it gives you a lot more confidence.Q: How is the team preparing for St. Cloud State? Do you guys take into account how you played them the first four times this year at all?Frykas: I think we need to start over from scratch; we can't take any team lightly. Whatever happened happened already, and it doesn't really matter if we beat them before or if we didn't. It comes down to winning these next two of three games. We'll go into this game and after that we'll go into the next one and just worry about each game one game at a time.The top-seeded Badgers host No. 8-seeded St. Cloud State this weekend in the opening round of the WCHA playoffs. The best-of-three series will take place at the Eagles Nest in Verona, Wis. Puck drop is set for 7 p.m. Friday and Saturday night, and Sunday's game, if necessary, will begin at 4 p.m. The winner of the series will advance to the 2011 WCHA Final Face-off at Ridder Arena in Minneapolis, March 4-5.
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BP PLC and its partners reported the successful drilling of the Itaipu-2 appraisal well on Block BM-C-32 in the presalt play offshore Brazil. The well was drilled to 16,000 ft total depth in 4,660 ft of water in the Campos basin and found a gross petroleum column of 58 net ft of presalt carbonate reservoir.
The appraisal well is an "aggressive step-out" from the Itaipu discovery well, which lies 4 miles to the northwest, said Bob Daniels, senior vice-president, worldwide exploration, Anadarko Petroleum Corp., which is a partner in the block (OGJ Online, Dec. 18, 2009). "The Itaipu-2 well established a fluid contact and appears to have successfully extended the accumulation 120 m downdip from the discovery. Accordingly, the appraisal well significantly increases the areal extent of the vast Itaipu field, and we believe incorporating the data from both the appraisal well and the original discovery well will increase our previous resource estimates for the field," Daniels said.
BP operates Block BM-C-32 with a 40% working interest. Anadarko, through a wholly owned subsidiary, holds a 33.3% interest, and Maersk Oil holds a 26.7% interest.


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Andrew Saint's review of A Guide to the New Ruins of Great Britain for the Times Literary Supplement has some nice things to say, and many criticisms.
For Saint, A Guide to the New Ruins of Great Britain is
no true guidebook at all but a ranting, panting travelogue eked out with provocatively scruffy little photographs ... [Hatherley] doesn't say much that is perceptive because he doesn't really look. He is in much too much of a hurry to place them in cultural context, say something flip, move on and weave his slashing narrative. Haste is both this book's virtue and its vice. It gives it a vitality and immediacy, but does not make for mature criticism ... its instant and local value is enormous. It destroys shibboleths, and its anger, zest and articulacy make one think.
Saint also remarks on the author's "macho façade and ... semblance of hectic movement." Saint, the general editor of the Survey of London, part of English Heritage's Research Department, then attempts "to define the shape of Hatherley's cultural baggage"
Architecture for Hatherley must be hard, sincere, obtrusive, if possible outrageous, by preference connected to the puritan heyday of the welfare state ... Just as for Betjeman the supreme experience might be evensong in a Comper church menaced by an urban motorway, so for Hatherley it is wandering through the deserted Sheffield Markets with hard-rock tracks in his ears, or talking to ex-punks who remember the last days of Hulme.
The Times Literary Supplement website is "under construction." This review appears in the edition of Friday 28 January 2011.
Benjamin Kunkel has written a lengthy article on David Harvey for the London Review of Books. Nominally a joint review of his recent books The Enigma of Capital and A Companion to Marx's Capital, it engages with Harvey's entire body of work, and especially his seminal The Limits to Capital.
Over recent decades, the landmarks of Marxian economic thinking include Ernest Mandel's Late Capitalism (1972), David Harvey's Limits to Capital (1982), Giovanni Arrighi's Long 20th Century (1994) and Robert Brenner's Economics of Global Turbulence (2006), all expressly concerned with the grinding tectonics and punctual quakes of capitalist crisis. Yet little trace of this literature, by Marx or his successors, has surfaced even among the more open-minded practitioners of what might be called the bourgeois theorisation of the current crisis.
In a critical review of John A. Hall's Ernest Gellner: An Intellectual Biography for The New Republic, John Gray opens by agreeing with Hall on one particular point—that Gellner was an exceptionally honest thinker:
John A. Hall concludes his account of Ernest Gellner by observing that his outlook on the world was austere. "But therein lies its attraction," he goes on. "Not much real comfort for our woes is on offer; the consolations peddled in the market are indeed worthless. What Gellner offered was something more mature and demanding: cold intellectual honesty." Brief personal impressions are rarely conclusive, especially when recalled after many years; but that Gellner was an exceptionally honest thinker is beyond reasonable doubt.
The Millions, one of the US's most-respected literary sites, has called The Art of Asking Your Boss for a Raise one of the most-anticipated books of 2011, noting that
We readers will have to deal with the fortunate burden of clearing shelf-space for another novel by Perec this spring, with the first English translation of The Art of Asking Your Boss for a Raise.
Visit The Millions to see the full list of recommended reading for 2011.


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You are hereEngland / Humberside Gazetteer
Humberside Gazetteer
Humberside Gazetteer
The drumming well located near to the church is reputed to foretell death in the family of St Quentin. The folklore relates to a story about a fourteenth century drummer called Tom Hewson, who was accidentally knocked down the well by a St Quentin squire. Read More »
Haunted Hull by Mark Riley
The bustling city of Hull has a long and distinguished history, but the area also harbours some disturbing secrets. Discover the darker side of Hull with this terrifying collection of spine-chilling tales from around the city. Read More »
Hull Masonic Hall, Dagger Lane
The following article entitled 'Hunt for Charlie the ghost at Hull Masonic Hall in Dagger Lane' was published in the Hull Daily Mail on 22 November 2014. It concerned a paranormal investigation to raise money for charity. Read More »
Nutwood Big Cat (2012)
Add to that the eerie atmosphere of dense woodland at night and it is enough to make the hairs on your neck stand on end.
But, that is what greeted two men who were out on a shooting trip in an East Yorkshire wood. Read More »
The Rudston Monolith
Standing in the Norman churchyard of All Saints Church, the Rudston Monolith is the highest standing stone in Great Britain at 7.6m (25ft) with a 5m circumference and an estimated weight of 40 Tonnes.
An experiment run by William Strickland in the 18th century suggests the stone may extend underground to a similar depth as it high above ground. Read More »
Skipsea Castle
Skipsea Castle dates from around 1086 and was one of the early Norman period Motte and Bailey Castles. The remains of the castle which was destroyed in 1221 when William de Froz II rebelled against King Henry III (born 1 October 1207 – died 16 November 1272) are traditionally thought to be haunted by a white lady, the wife of Drogo de la Bouerer, who founded the castle. Read More »
The Manchester Arms, Hull
The following story entitled 'Ghost captured on video at Manchester Arms pub in Hull's Old Town' was published in the Hull Daily Mail on 9 November 2012.
WHEN the landlady calls time, she is the visitor who refuses to leave. Read More »
The Screaming Skull of Burton Agnes Hall
The skull at Burton Agnes Hall is another famous screaming skull, although its exact whereabouts in the hall is unknown. It is thought to reside behind one of the walls, having been bricked up and forgotten about years before. Read More »
Watton Abbey
A private residence, Watton Abbey is a Grade I listed building dating from fourteenth and fifteenth centuries and built on the location of Watton Priory, which was a Gilbertine double monastery founded by Eustace fitz John (died 1157) in 1150, as a penance for having fought in the Battle of the Standard (aka Battle of Northallerton) (23 August 1138) on the Scottish side. Read More »


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Jay Ahr's effortlessly cool jumpsuit is perfect for evening dressing. Fusing a lightweight linen-blend top with tapered crepe pants, this relaxed piece is guaranteed to make a statement. Style yours with metallic accents to punctuate the fine gunmetal chains in the cascading fringe.
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Product code: 349186 - Need help? Contact us


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Ashes 2013-14 explained: The key questions answered
England have travelled to Australia to defend the Ashes they won at home last summer.
With England looking for a fourth successive series victory, the first Test in Brisbane on Thursday begins only 88 days after Alastair Cook lifted the urn at The Oval.
BBC Sport explains the reasons for the swift turnaround, how the series down under will differ from the one in England, and where victory would rank for England.
Why are the teams playing again so soon?
England would usually travel down under 18 months after hosting Australia, but this instant return series is the result of a desire to alter the Ashes cycle.
The 10 Ashes Tests of 2013-14
In England
1st Test: England won by 14 runs, Trent Bridge
2nd Test: England won by 347 runs, Lord's
3rd Test: Match drawn, Old Trafford
4th Test: England won by 74 runs, Chester-le-Street
5th Test: Match drawn, The Oval
In Australia
21-25 Nov: 1st Test, Brisbane
5-9 Dec: 2nd Test, Adelaide
13-17 Dec: 3rd Test. Perth
26-30 Dec: 4th Test, Melbourne
3-7 Jan 2014: 5th Test, Sydney
The last three Cricket World Cups (2003, 2007 and 2011) have immediately followed Ashes series in Australia.
As a result, both teams went into the most important one-day tournament straight after their biggest Test series.
In 2011, the World Cup began only 16 days after England's final one-day international in Australia.
This issue is further compounded by the fact that the next World Cup is scheduled to be held in Australia and New Zealand in early 2015, soon after when an Ashes tour would ordinarily have concluded.
The altered Ashes scheduling does not end this winter. Australia would usually tour England two-and-a-half years after hosting the Ashes, but they will next visit the UK in 2015, only 18 months after the forthcoming series.
This is to ensure that Ashes series in England remain in odd-numbered years so as not to clash with the Olympic Games or major football tournaments.
From there, England are not scheduled to tour Australia until 2017-18, with Australia returning in 2019.
England won 3-0 at home. Will this series be different?
England were helped to success in the summer by the home advantage of slowish, turning pitches.
"England have two question marks remaining over their final Test line-up with the identity of their third seamer yet to be decided and a slight concern over the fitness of wicketkeeper Matt Prior.
"England will not want to take any risks on Prior but it is my understanding that his calf tear of the lowest grade of severity, and that he should therefore be fit for the first Test.
"It is crucial for England that Prior does play, as much for his skills as a world-class all-rounder as for his leadership and influence in the dressing room."
On these wickets, off-spinner Graeme Swann was the stand-out performer in the series with 26 scalps. James Anderson and the rest of the pace attack found reverse swing in the early part as Australia's batsmen struggled.
The home side were still successful on a greener pitch in Durham, but on the fast, bouncy wicket of Old Trafford they struggled and probably would have lost had it not rained on the final day.
It is likely that England will encounter pacier wickets down under, although they may not be as lively as Australian surfaces of the past.
England have looked to counter that threat by selecting four tall pace bowlers - Stuart Broad, Steven Finn, Chris Tremlett and Boyd Rankin - in their squad.
But former England paceman Steve Harmison said he would be "amazed" if Australia ask for bouncy pitches to be prepared and Cricket Australia boss James Sutherland says the hosts will not look to exploit home advantage.
As well as having their own selection issues to resolve, England will also be facing hosts who are much more settled than in either of the two previous series.
What changes might England make?
If Matt Prior overcomes a calf injury and Finn is chosen as the third pace bowler for the first Test in Brisbane, then England will field nine men who played in the corresponding game three years ago.
Consistency of selection has been a contributing factor to England's recent success, but they have yet to adequately replace the two other men who played in that Brisbane Test of 2010 and have since retired - Andrew Strauss and Paul Collingwood.
Nick Compton and Joe Root have both been tried as Strauss's successor at the top of the order, with mixed success and, after Michael Carberry's hundred in the game against Australia A, the Hampshire left-hander looks set to be the next man to be given an opportunity.
That would see Root return to number six, where England have also tried the likes of Eoin Morgan, Jonny Bairstow, Ravi Bopara, Samit Patel and James Taylor since Collingwood's final bow.
Finn is one option to take the third seamer's position that was filled for much of the summer by Tim Bresnan, who has since suffered a back injury.
Finn is vying with Tremlett and Rankin, but none of the trio have used the three warm-up matches to make a convincing case for inclusion in the Test side.
Bresnan is with the England squad in Australia as part of his recovery from surgery and Cook said in October that the Yorkshire man may be fit for the first Test, although it is more likely he will be available later in the series.
And what about Australia?
Unsettled in their previous two Ashes hammerings, Australia look a more content unit now.
Though heavily beaten last summer, Australia at least left England with greater clarity about their batting line-up in particular.
"Whether the squad is strong or not will be determined by how well they play, but for the first time in maybe 18 months, it hasn't taken the selectors long to find the final XI or the final XII.
"Bailey is no great surprise - he had a huge run scoring tour in India in the one-dayers, so the batting is looking as stable as it has for quite a long time.
"Mitchell Johnson has been picked more on his one-day form, like Bailey, but he has bowled quite fast and was a big part of Australia winning a couple of games in India."
Veteran opener Chris Rogers made a maiden Test century and will be joined by David Warner despite the latter's high-profile disciplinary issues.
Shane Watson scored a hundred at The Oval at the troublesome number three slot, as did Steven Smith at number five, to form a middle order either side of captain Michael Clarke, while the experienced George Bailey's impressive one-day form looks set to earn him a Test debut at number six.
The continued fitness that allowed pace bowler Ryan Harris to take 24 wickets in England was another boost for Australia, but injuries have hit fellow seamers Mitchell Starc, James Pattinson, Jackson Bird and Pat Cummins.
Still, Australia have been able to call on a resurgent Mitchell Johnson, the mercurial left-armer capable of bowling above 90mph.
Johnson has 205 Test wickets to his name but struggled for most of the 2010-11 series. However, he looked back to his best in last summer's one-day internationals and could prove a handful.
Will DRS again be a factor?
What are Snicko and Hot Spot?
Snicko uses soundwaves to try to detect if the batsman has edged a ball, while Hot Spot uses thermal imaging cameras. While there have been instances where a faint edge has not shown up on Hot Spot, it is hoped the two could work together to create a better decision review system
The reliability of the Decision Review System was regularly called into question in England this summer, with Jonathan Trott, Kevin Pietersen and Usman Khawaja some of the batsmen on the wrong end of controversial decisions.
Many of the problems surrounded Hot Spot, the infra-red camera used primarily to detect whether the ball has struck the bat.
In October, inventor Warren Brennan said he did not believe that it would be used, but it has since emerged that Hot Spot may be deployed alongside Snicko and the more established ball-tracking technology.
However, the biggest change that will definitely take place is the number of failed reviews that each team will be allowed per innings.
Previously, both sides were allowed only two unsuccessful reviews for the whole innings. Now, that number of reviews will be reset at two after 80 overs.
How big an achievement would an England victory be?
After suffering eight successive Ashes series defeats between 1989 and 2002-03, England are enjoying their most successful period since the late 1970s and early 80s.
Victory in Australia would give England a fourth successive Ashes series win for the first time since the 1880s and 90s, when they won eight in a row.
Success in Australia would also be the first time in more than 80 years that England have won successive series down under, with the last twin victories coming in 1928-29 and 1932-33, the second of which was the infamous Bodyline tour.
The 2013-14 series also carries a potential statistical significance for both sides. After 67 Ashes contests, England and Australia have won 31 series each, meaning both sides have the opportunity to nudge ahead.
Overall, however, in 314 Tests, Australia have won 123 to England's 103.
First Ashes Test: Australia v England
- Venue: The Gabba, Brisbane
- Date: 21-25 November
- Start time: 00:00 GMT
Coverage: Ball-by-ball Test Match Special commentary from 23:30 GMT on BBC Radio 5 live sports extra, BBC Radio 4 Long Wave and via the BBC iPlayer Radio app, BBC Sport website & BBC Sport app; updates on BBC Radio 5 live; live text commentary on BBC Sport website, app & mobile devices


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Flying from Munich
Flights from Munich with airberlin connect to multiple locations within Germany and across Europe. Munich Airport (MUC) is the international airport serving Munich and is located about 28 kilometers northeast of the city. It is the second busiest airport in Germany.
Munich Airport Information
Munich Airport offers a wide range of entertainment, shopping, and other services to make visits more enjoyable for customers. With over 200 shops, Munich Airport has something for everyone. Have a meal or a drink at one of the many restaurants or bars, or use your free time to get into shape at the fitness studio. Other services include wireless internet, banks and currency exchange, shower facilities, and a health clinic.
Getting to the Airport in Munich
Travelers who choose to drive themselves to the airport have the option of short and long-term parking. A taxi to Munich Airport takes about 45 minutes from the city. The S1 train to Munich Airport takes about the same amount of time and run frequently. You can also take a bus to Munich Airport from surrounding cities.
Arriving in Berlin
Uniquely molded by history and the stalwart resolve of its citizens, Berlin is a city of startling variety and beauty. Germany's capital is home to nearly 200 museums and over 400 art galleries, as well as boutiques, restaurants and nightclubs.
First Time in Berlin
There are a number of iconic attractions within the city that you shouldn't miss when you first travel to Berlin. Among them is the Reichstag, home to the German Parliament since the turn of the century and capped by Norman Foster's awe-inspiring glass dome.
Your Berlin
History buffs should purchase tickets to Berlin's Schloss Charlottenburg, the most luxurious of nine surviving royal dwellings dating back to the reign of King Friedrich I. Gastronomes will love Die Hackeschen Höfe, a bustling collection of over 100 stores, restaurants and cafes.
Making the Most of Your Berlin Holiday
For the best weather during your holiday, plan for your flights to Berlin to arrive during its mild summer season between May and September. Cheap hotels in Berlin are generally easy to find near the Kurfürstendamm and around the city center. Remember to log on to airberlin.com for a great selection of flight deals to Berlin. Find attractive fares year-round to Berlin on airberlin.com


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- Filed Under
For the last few years, Kenny Chesney's love of the beach-bum island lifestyle has been teetering on the brink of shtick. While Chesney's devotion to the Virgin Islands is sincere, it's just a fact that Jimmy Buffett has ruined that scene for everyone.
And when he teams up with The Wailers - The Wailers! - for a song called "Spread the Love," it feels as if Chesney has finally gone overboard. Instead, the most wildly successful country singer of his generation has managed to make what might be his best record ("Spread the Love" notwithstanding).
Chesney does it by writing some of his most personal songs, all of them about people he's met on St. John's Island or the friends who have traveled there to party with him. "Lindy" is a lovely character study of an island regular, with a classic James Taylor vibe. "Happy on the Hey Now (A Song for Kristi)" is about a friend who died young, and even though it's filled with cliches, Chesney manages to also fill it with real emotion.
On "Marley" (about his love for another Wailer, the late Bob Marley), Chesney eases through a song about exactly why the islands move him. It's an atypical country song in nearly every way, including a reference to Ernest Hemingway, and it feels like a cool breeze across the bow.
- Jeffrey Lee Puckett, The Courier-Journal


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21-22 Wellington Quay, Temple Bar, Dublin 2
Fitzsimons Restaurant with a full bar overlooks the River Liffey. We pride ourselves on the quality of our food at reasonable prices. Our extensive menu contains an exciting selection of modern Irish and European dishes. Serves begins at 12.00 to 10.
Read more about Fitzsimons | View Map of Area | Photos


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Jason Aldean and Luke Bryan have already teamed with Duck Dynasty's Willie Robertson and baseball star Adam LaRoache along with a few other pals as co-owners of Buck Commander, a hunting lifestyle business that focuses on deer hunting and apparel. Could the restaurant business be next on their resume?
According to a report on steamboatsprings-realestate.com, the four pals along with baseball players Jeff LaRoche and Andy LaRoche are opening a chophouse in Steamboat Springs, Colorado. The restaurant, E3 Chophouse, located on the banks of the Yampa River near premiere skiing and mountain biking destinations, is said to have a "great menu with reasonable prices."
Steamboat is an area best known for its outdoor lifestyle. While well-known for its skiing, the area also offers fly-fishing, hunting, a number of restaurants and a western ranching heritage. The area also hosts MusicFest annually, a six-day music festival featuring top names from Americana music.


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At the Open Hardware Summit just before World Maker Faire New York, Shawn Wallace introduced me to Chi from RedBearLab. Shawn knew that I'd developed a very healthy obsession with Bluetooth Low Energy; some time ago, Tom Igoe and Alasdair Allan clued me into the fact that it was not only a simple, low power communications option for Arduino and other devices, but it also avoids Apple's requirement that you be in their MFi licensing program to do Bluetooth communications with an iPhone or iPad.
I'm happy to report that we're now carrying Chi's shield in the Maker Shed. Only $35, which is pretty inexpensive for a shield with the radio right on board. (Compatible iOS devices are iPhone 4S, 5, iPad 3rd generation, and iPod Touch 5th generation).
And what's more, I doubt this is Apple-only. Any device with Bluetooth 4/Bluetooth Low Energy can join in the fun. Android Jelly Bean and later has it (hardware willing), but what I've been told is that the only Android devices that have an SDK are those from Google-owned Motorola Mobility: Motorola Bluetooth Low Energy API. But I'm not so sure of that. After all, the ADK 2012 hardware includes a BLE chip, and my (non-Motorola) Nexus S and 7 seem to be happy to talk to that gadget. Well, getting the Bluetooth Low Energy Shield for Arduino to talk to Android is something I'll have to try another time. Getting it to work with iOS was easy. Perhaps a bit too easy…


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I'll be in Asheville for two weeks early in September and I'd like to spend 3 or 4 nights hiking and camping. Does anyone have any recommendations on any spots or trails?
Lots of great opportunities around. The Roan Highlands section of the AT is really nice. Best views in the East I have seen. Plenty of other good AT sections south to the Smokies too.Linville Gorge is nice and about an hour away. Its steep going down and back out and lots of beautiful terrain.Shining Rock, Art Loeb trail area is not far; I have hiked only parts but I think that whole area is nice.The Smokies. Great access for you out of Big Creek allow you to quickly access some high ridges. Mt. Cammerer tower is worth going up to. Plenty of connecting trails down to Big Creek and over to Mt. Sterling so you can make a loop. You could also hike from Big Creek all the way to Newfound Gap and get a shuttle back. Really edless options in the Smokies.You'll probably like Ashville too. Spend some time in the old neighborhoods.
I love the Art Loeb trail; a friend of mine and I yo-yo'd it MLK day weekend earlier this year and loved it. Should be beautiful in September as well (and a bit less cold). There's also lots of wonderful camping and day hikes in that area off the Blue Ridge parkway (my favorite camping spot is one in Graveyard Fields - blueberry and blackberry bushes galore). Additionally, there's some nice little loops in Shining Rock Wilderness (in which half the Art Loeb resides). www.hikewnc.info has maps and descriptions for lots of good hikes in the area; I plan on frequenting it over the next couple of months myself.The section of the AT in the Smokies is also lovely, you really should check it out if you haven't before. In March I hiked a nice little loop from Newfound Gap along the AT to the north end of the park and then back around the north-northeast border of the park back through the Mt. LeConte area.edit: Newfound Gap, not Newfoundland Gap...
Edited by riemannia on 08/08/2012 00:11:17 MDT.
I agree with the AT comments. Last year my wife and I hiked Newfound Gap to Davenport Gap and there were some great views especially around Charley's Bunion. As noted above, Mt. Leconte is in that area as well and I highley recommend it.
Thanks for the recommendations! I'll be checking these out.
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PAWTUCKET – All Jason Repko can do is sit tight, hoping that his chance at parole from baseball's version of no-man's-land comes sooner rather than later.
Repko's placement on the disabled list on July 7 has absolutely nothing do with the hamstring strain that has hamstrung the 31-year-old outfielder to the point that even the player doesn't know when he'll be activated. For someone who is completely healthy, Repko finds himself the victim of a numbers game due to the Red Sox being presently flush with outfielders at the major-league and Triple-A levels.
It cuts deeper as Repko has resigned himself to the fact that chances of him seeing the field for Pawtucket hinge upon some sort of player movement, and even then there's no guarantees that he becomes the choice to fill the void.
I'm just waiting; I have no control," sighed Repko on Monday afternoon. "All I can do is show up to the ballpark each day, get my work done and see how all the pieces fall. Whenever that time is, I'll be ready."
Finding himself buried on the depth chart at the minor-league level can't be an easy pill to swallow, but Repko says he understands why he's the odd man out at this particular juncture.
"There are so many outfielders here (in Pawtucket)," he said, the group ahead of him ranging from veteran types (Scott Podsednik) to players on the 40-man roster (Ryan Kalish, Che-Hsuan Lin) and 20-somethings (Alex Hassan, J.C. Linares). "If something happens now or next week, I have to keep myself ready."
Starts and stalls have been commonplace in Repko's season. After four games with the PawSox, he was summoned to Boston on April 15 in what proved to be a short and painful stay. At a time when the Red Sox were unsure when Carl Crawford would return and the loss of Jacoby Ellsbury was still relatively new, Repko had a golden chance to stake his claim.
Then came April 20, which saw Repko suffer a left shoulder separation while making a diving catch. He was sidelined for close to two months before being officially outrighted to Pawtucket on June 22. Repko appeared in 10 games since his reclassification, hitting just .244. Previously with the Dodgers and Twins, Repko last appeared in a game with Pawtucket on July 5 – two days has before being placed in limbo.
"The more you spend on worrying about stuff like that, the harder it is," said Repko, generally one of the more upbeat guys in the clubhouse. "When you know the situation, it makes it easier. You see all the moves being made and I know how it is. You have to wait."
One of the players currently blocking Repko's path to playing time is Kalish. Monday saw the outfielder undergo an MRI just to make certain that there was nothing structurally wrong with the knee that went crashing into the wall, the result of an amazing grab he made in center field last Friday.
"With the knee, structurally you never know what's going to happen, but (the MRI) was good for everyone," said Kalish.
Kalish expressed optimism about avoiding a trip to the disabled list, yet he knows there's no sense in quickly hurrying back if he's still experiencing soreness.
"I don't know what's going to happen, but what's the difference between three or five days?" Kalish noted.
Added PawSox manager Arnie Beyeler, "We're just going to make sure he's okay and give him time to come back."
Even though this lull is the result of an on-field setback the break figures to serve Kalish well, especially when taking into account the steady diet of action he's received following nearly a year off due to neck and shoulder ailments.
"My body has been through a lot and it might be another year until I feel really good," Kalish said, "but I'll be back out there soon."
Beyeler was able to enjoy a rare in-game experience on Sunday upon getting ejected, that of viewing the action on television. The skipper ended up watching the final seven innings of a 14-inning marathon from the comforts of his office, which allowed Beyeler to pick up on tendencies that aren't as apparent while sitting in the dugout.
"You see (Ryan) Lavarnway catching balls behind the plate and the release points of (Daniel) Bard and (Mark) Prior along with the mechanical things we talk about," Beyeler said. "It was nice to see things from a different angle."
EXTRA BASES: Shortstop Jose Iglesias was in the leadoff spot for the second straight game. … Mount St. Charles graduate and Cumberland native Meghan Lydon performed the national anthem. Lydon played tennis for the Mounties. … The PawSox welcomed back lefty reliever Will Latimer on Monday while infielder Ryan Dent was optioned to Double-A Portland. Latimer's previous stint with Pawtucket saw him go 0-2 with a 4.50 ERA in four appearances.


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A Thanksgiving Tryptophantastic Triple Feature
Thanksgiving Week is upon us at last. Tis the season to wrestle your way through the airport, fight with your siblings, and stuff your face with, well, stuffing, among other delicious delicacies. Once the big meal has finally been prepared, served, and devoured, change into some sweats and collapse onto the couch for three Thanksgiving-themed flicks that are sure to help you pass that pesky time in between eating and eating again, later that night.
Planes, Trains and Automobiles
This Thanksgiving classic is John Hughes' first foray into films starring adults as opposed to teenagers. It's also arguably the most beloved Thanksgiving movie of all time. In case you haven't seen it, Steve Martin is hilarious as Neal Page, an uptight but all-around good guy who just wants to get home in time for Thanksgiving dinner with his family. Cue John Candy as Del Griffith, shower-curtain-ring-salesman of poor Neal's nightmares, who, wouldn't you know it, ends up along for the ride. While this quintessential 80s comedy is undeniably slapsticky and more than a bit silly, it's also smart and surprisingly emotionally poignant.
A Charlie Brown Thanksgiving
This family-friendly Thanksgiving charmer is one of my favorites. It's on TV every year around this time, so be sure to set your DVRs. The sweet little story involves Peppermint Patty and her friends inviting themselves over to Charlie Brown's for the big meal. When Charlie attempts to throw together a feast to please her, the results are less than satisfactory. Of course, at the end of the day, everybody learns a little something about what it means to be thankful. Be sure to have some popcorn and jelly beans on hand in case you want to eat along.
On the complete opposite end of the spectrum is this low-budget horror gem. Apologies, but I had to include this admittedly horrific (in every sense of the word) independent "film" for those of you seeking something a little less saccharine for your post-meal enjoyment. This movie will especially appeal to those of you who lace your pumpkin pie with something a little bit stronger than, say, cinnamon. The plot, and I use that word very loosely, centers around a weapon-toting, fourth-wall-breaking, homicidal turkey on a college-student slaughter spree over Thanksgiving break. I mean, what's not to like about a movie whose official website instructs you to "laugh at it because it's bad." Note: as you can probably surmise from the poster, this is one to watch after the babies and grandparents are long since asleep.
Unlike Christmas, Thanksgiving is not a holiday replete with accompanying movies. There are, of course, many that I didn't mention, which isn't to say that films like Home for the Holidays, Pieces of April, or The Ice Storm are any less worthy of your dedicated digestion (heh heh). Not to mention the hilarious (and now somewhat nostalgic) Thanksgiving episodes of shows like Friends and Gilmore Girls.
What are the movies and TV shows you like to watch every year?
Images via amandalaura.blogspot.com, drafthouse.com, abetterbagofgroceries.com, thecitrusreport.com


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Curb issues for sidewalk users
South Carolina was the first state in the U.S. to pass a disability act in 1963, said Sandy Hanebrink, director of Touch The Future in Anderson. Born physically able, Hanebrink said she had to learn how to take on new challenges when she became disabled. Accessibility to public transportation is an issue to her and many disabled who cannot get to it when sidewalks are not made to code. Curbs where yellow mats are part of the corners were not properly installed at many locations around Anderson and need to be fixed she said.


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Posts Tagged 'Enlisted Ratings'
June 3rd, 2014 at 8:06 am
Sunday Final Ratings: 'The Bachelorette' Adjusted Up
Sunday final broadcast primetime ratings.[Read-More]
June 2nd, 2014 at 8:39 am
TV Ratings Sunday: 'Enlisted' Flat in New Time Period, 'Cosmos', 'Believe' & 'Crisis' Rise + 'The Bachelorette' Slides
ABC & FOX tied for number one with adults 18-49, but CBS led with total viewers.[Read-More]
May 20th, 2014 at 12:57 pm
'Enlisted' Final Episodes to Air on FOX Beginning Sunday, June 1
FOX will air the final four episodes of now-cancelled Enlisted[Read-More]
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Freshman comedies Dads, Enlisted and Surviving Jack have been canceled by FOX[Read-More]
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Friday Final Ratings: 'Last Man Standing', 'The Neighbors, 'Dateline' & '20/20' Adjusted Up; 'Hannibal' & 'Hart of Dixie' Adjusted Down & Final Basketball Numbers
Friday's final broadcast primetime ratings.[Read-More]
March 29th, 2014 at 8:49 am
TV Ratings Friday: 'Enlisted' Falls as 'Raising Hope' Rises + 'Hannibal' Matches High
CBS was number one in adults 18-49 and with total viewers.[Read-More]
March 26th, 2014 at 11:34 am
FOX Announces Premiere Dates for 'Kitchen Nightmares', 'Gang Related', 'Hotel Hell', 'MasterChef' & More; 'Enlisted' Pulled From Schedule
FOX is announcing additional spring and summer premiere dates for 2014.[Read-More]
March 24th, 2014 at 1:42 pm
Friday Final Ratings: 'Shark Tank' & '20/20' Adjusted Up; 'Hart of Dixie' Adjusted Down + Final NCAA Basketball Numbers
Friday's final broadcast primetime ratings.[Read-More]


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There's a new free app for Apple available today. Download Flow Free free through iTunes.
App Description:
Flow Free is a simple yet addictive puzzle game.
Connect matching colors with pipe to create a flow. Pair all colors, and cover the entire board to solve each puzzle. But watch out, pipes will break if they cross or overlap!
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Cafe Luna offers more to love on Fifth Avenue South
Cafe Luna
Where: 467 Fifth Ave. S., Naples
Hours: 11:30 a.m. to 4 p.m. daily for lunch; dinner hours 4 to 10 p.m. Sunday-Thursday and 4 to 11 p.m. Friday and Saturday
Prices: Starters $4.99-12.99; lunch entrees $7.99-12.99; dinner entrees $18.99-27.99
Information: 239-213-2212 or cafelunanaples.com
Literally changing the face of Naples' Fifth Avenue South with its blockbuster "A Veally Good Deal," Cafe Luna has expanded, just in time to celebrate its fifth anniversary.
Rated as one of the top 10 restaurants in Naples by U.S. News & World Report, it is nestled in between Paddy Murphy's watering hole and recently opened Osetra Champagne caviar bar.
Business has steadily increased since opening, said Ed Barsamian, who owns and operates the smoke-free eatery with working partner Shannon Tiwell and a staff of 35.
The revamped bar area now wraps around to seat five patrons outdoors, for a total of 15 seats. Slender, elongated pendant lighting and three flat-screen televisions offset the new granite-covered bar top.
With cozy table seating for 50, the indoor bar and dining room features boldly hued wall colors of deep orange and red selected by Barsamian that serve as a backdrop for the commissioned artwork of local artist Steve Baffa.
Larger-than-life oil paintings of Sinatra and the cigarette-toting Rat Pack imaged as superheroes share space with another art piece with ruby red lips that beckon patrons to "mangia," or "eat" in Italian.
And eat you can in an adjacent new dining area that seats 32 and is opened to the outdoors. Guests can dine watching a flat-screen television or the diners seated on the outdoor patio.
Getting down to how Cafe Luna has made its presence known and felt in the competitive restaurant arena, it's the "deal" that has hit big.
With an eye-popping price of $29.99 for two dinners and a bottle of wine and a nice view of Fifth Avenue, Cafe Luna has amassed a roster of loyal customers.
Look around at restaurant advertising and see the adage at work that imitation is the best form of flattery, as more and more restaurants offer two-for "deals" for similar or the same prices.
But serving numbers such as 196 on a recent Wednesday lunch or 220 to 300 for dinner shows folks are eating up the deal not only for the savings to their wallets but also because the food is just that good.
Compare the marinara sauce to the equal of any found on plates on Fifth Avenue that demand $24 to $30 per dish, thanks to house recipes and the culinary skill of Chef Eddie Marchese, the former executive chef of Maxwell's and the Waterfront Grill, who has played a critical part in the new expansion.
With white linen-covered tables overlaid with glass, a friendly but not overbearing wait staff serves up a basket of warm bread, olive oil and grated cheese — enough to munch on while deciding on your feast.
Selecting one of the seven $29.99 deal entrees from chicken or eggplant parmesan to penne Bolognese is not that easy; they are all superior in portion and taste.
Upgrades for $5 more expand choices to shrimp scampi with linguine, gnocchi or veal parmesan with spaghetti. Upgrade by $10 and select from steak Diane (10-ounce black Angus cut), veal saltimbocca or pan-seared scallops.
Upgrade to any $40 or less bottle on the wine list for $10 and add small appetizers for $3.99 such as fried calamari, mozzarella sticks and more.
"The way the deal is structured and managed, it is profitable for us; we turn tables quickly, offer upgrades and offer the freshest food. This has made Fifth Avenue more affordable to locals and visitors alike," said Barsamian.
"I would like my fellow businessmen to be as busy as I am — that is my wish," he added.
And to prove the viability of the regular menu chosen by more and more patrons — and which is served exclusively two times a year, on Christmas and New Year's Eve — on that last day of 2010, Barsamian said he served a whopping 720 covers.
Diners those days chose from items such as antipasti ($7.99-$12.99), soups ($4.99-$6.99), salads ($5-12.99), to house specialties ($18.99-$26.99), pasta ($15.99-$21.99), pollo ($18.99-$22.99) and frutti di mare ($23.99-$27.99).
The "lunalicious" lunch menu offers salads ($5-$12.99), soups ($4.99-$6.99) appetizers ($5.99-$9.99) to sandwiches ($7.99-$11.99) and lunch plates such as spaghetti and meatballs and rigatoni vodka ($8.99-$12.99).
Hand-tossed pizza is made fresh daily, and a gluten-free menu is available.
A lunatic happy hour with half off all wells, premium liquors, beer and wine bottles from 4 to 6 daily with a choice of six $2.99 bar items is sure to fill the bar seats indoors and out.
Opened in January 2007 with 50 seats and no outdoor dining, Barsamian added 50 seats on the covered patio by the end of that year.
In April 2010, the restaurateur rented space that became available next to his patio, which upped his total seat count to 150 — enough to get a liquor license and warrant the new expansion to its current 182 seats.
"I owe a debt of gratitude to Phil McCabe, who has been a tremendous help in many ways. Life really is a 'veally good deal.' Come in and try it," added Barsamian.
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Jan. 7, 2013
• Watch Eaves News Conference
MADISON, Wis. -- Wisconsin men's hockey head coach Mike Eaves spoke Monday after the team earned its second series sweep of the season against Alaska Anchorage. UW remains on the road next weekend for a series with Minnesota State, Jan. 11-12.
Coach Eaves and the Wisconsin men's hockey program also unveiled commemorative jerseys to be worn Feb. 17 in the OfficeMax Hockey City Classic against Minnesota.
Archived video of the media session is available through the link above and a complete transcript of Eaves' remarks can be found below.
Eaves: At this time we'd like to introduce a new jersey for the 2013 OfficeMax Hockey City Classic that we'll wear February 17th in our game against Minnesota at Soldier Field. The jersey is a reproduction of the jersey worn by our team in 1931-32, and it is something of a continuation of our season-long celebration of our program's history in the 50th season of the modern era. We brought in our special captain John Ramage/model. He's going to stand up and give you a look-see at the jersey for our game. Hands on your hip. This is a visual of it. The guys are really excited about this jersey. We showed them earlier. It's something we look forward to wearing in that game. (It) should be a lot of fun.
Question:Will the coaches be wearing any articles of clothing from the early '30s? Eaves: To be determined. To be determined. Maybe a chapeau of some sort from the '30s. I can guarantee you it will be warm.
Question: Why is your team playing so much better on the road than it would appear it has at home? Eaves: It all has to do with getting people back, getting healthy, those types of things. It's funny how people say, well, what's different? We've got our people healthy and not suspended. Our game makers are back in the game and providing offense, and we have that in our ability now. So that's a key reason why we're on this little run that we're on right now.
Question: Those that didn't see the series or didn't hear the series, how would you recap those two days? Eaves: Friday night, the big thing was we scored five goals. We didn't play particularly well in terms of system play, and it was unusual for us to watch video and watch the line of Dahl, Little and Little play the systems that we played so poorly. They were off their assignment. It was kind of a bad game that way. But it was the first game that we hadn't played decently and found a way to win. The reason for that is we scored five points. On Saturday it was just the opposite. We earned that game. We deserved that game. We played very well. There was a moment in the first period where there was a shift and a half where Anchorage came out to establish itself physically, and we just answered the bell. From that moment on, a lot of things we did were very good. We only scored one goal -- I haven't seen the chances yet, but I just felt during that game we did so many good things. It was good to play that way and find a way to win.
Question: How easy is it for your team to tap into the energy that a Jake McCabe might bring back to you? Eaves: Just spoke with Jake this morning at 10:30 this morning. It was 10:30 in Russia at night. So I'm not sure he'll remember our conversation. Any time a player goes through an experience like that -- I remember when we came back from Finland or when Steps And rammer came back from their win, they're on a euphoric high that first weekend. They will play very, very well is what history tells us. Until a couple weeks later, it hits them physically, mentally and emotionally. But he will bring into the locker room that level of play that I hope will rub off. You watch those games, the pace at which those games are played is phenomenal. I would like for him to be able to come in and keep playing at that pace and hold on to that pace as long as he can and talk about the things that they did over there. He'll have that intrinsic knowledge of what it takes to be a winner at a high level and bring that into our locker room.
Question: Do you expect Jake (McCabe) to play this weekend? Eaves: Yes. He wants to play. He will play. Physically, he says I'm healthy. I've just got to get used to the time zone.
Question: Also, a follow-up, Chase Drake took his place this past weekend and played pretty decently. You've got seven guys there. Eaves: He definitely played well enough that there's competition to stay in that top seven. So Chase did a nice job for us. I don't think we missed too much of a beat by having him step in. To his credit, he hasn't played in a long time, and he did a nice job.
Question: Kind of trying to find a way to put this. How would you assess Eddie Wittchow and Kevin Schulze at this point, two of your freshmen defensemen, who have played a lot, and their numbers don't suggest they'd jump at the sheet at you, but they've certainly entrenched themselves in the lineup. Eaves: You can see growth in their play. They certainly fumble around once in a while, especially when we go into new buildings that they're not used to. You can see their eyes are lit up a little bit. But their understanding of our system, their understanding of the pace of our game, their understanding of the strength of the men they're playing against, they're all getting used to that and understand what they have to do. They keep rising, Andy, in their understanding and their ability to play.
Question: You mentioned Jefferson's line a little while ago. The fourth line played pretty strongly on Saturday too. The top two lines, those are two pretty potent lines that seem to be kind of clicking right now. Eaves: No question. Derek Lee's line with Mersch and Joseph LaBate are playing as well as I've ever seen those guys playing together. Saturday in the game, Joseph was playing at another level, and there's real good chemistry between Lee and Mersch. We haven't seen the top end of Zeng's line yet. If you watch them practice, when they have the puck, they can do real special things. We haven't maximized that in games yet. Our challenge to them a little bit is we want to be able to rely on them to put them out late in games when we're up by a goal, knowing they can get the job done and not give up a goal. They have the skill to score. Like on Friday night, they scored the empty net goal. We want to know they're able to play real well with and without the puck so we can put them out there in key situations.
Question: Has the ship been turned yet? Last time I bring up that cliche. Eaves: We're undefeated in eight. I would say the wind is at our sails. Is it maxed out yet? I would tend to say no. I would want to say no. I would like to see us play even better, but the wind is definitely in our sails now, and we're going for it.
Question: Looking back at the start of the season, was Mankato kind of the low point for this team in terms of what it learned and losing -- not being able to even get a point out of that series here in Madison? Eaves: That series hurt in terms of not getting points, but I went back and looked at that series. Friday night, we out chanced them significantly. Saturday we out chanced them by a little bit, and we lost both games 4-2. I don't think we played, by any stretch, our worst hockey. We just couldn't score, and we didn't get goals at the right time. I'm hoping with this wind in our sails, we're going into an environment we know is a tough environment, but what a great environment to win in. I'm hoping our guys will go in there and play well.
Question: You've been involved at this level for a long time. In your dealings with the USHL, are you at all surprised at the success Mankato is having right now under Mike Hastings, their first year coach? Eaves: Mike's a good coach. I think, when you come in and there's new life and new hope and there's energy, and they got off to a good start and just carried that on. I'm not surprised. Sometimes that's the way it happens when you have a change in coaching staff.
Question: When you look back at the Mankato series, you were missing Mark, you were missing Nic. Is there a mentality with this team that would seem to benefit them, knowing that you have them back, that you weren't at full strength at that time, that you're a different team now? Eaves: Well, I think, just having those two guys back in and the way we're playing together. I don't think it's just one thing. I think it's the way we're playing together with those guys back in the lineup. We have our full complement. We have good chemistry. We have the wind in our sails. Those are things we're going to rise to this weekend.
Question: Have you heard at all about ticket sales for the outdoor game? Eaves: No, I haven't. We talked about it on the road up in Alaska, but there wasn't any mention of what it was at the time. It's getting closer. I'm sure we'll hear a lot more about it. I hear the guys talking about it in the locker room already. They've got it circled on the date there on the calendar. Just once again, we don't have any breaks during the second half of the season. I think that will be, you've heard us call, an emotional energizer in the middle of a long stretch of games that I think will really elevate the emotion we'll need to carry us through to the second half.
Question: One more too. The National Hockey League is going to start up again. I know the guys in the locker room are happy about that and fans everywhere. How about you? Eaves: It will take up more of my TV time, I know that's true. But it's a really good thing. You know what, how it affects us is that our guys get to watch guys at the next level, and we use that. We watch games of pro hockey, and we ask guys who they emulate at the next level. Now they'll be able to watch those guys and see what the top end is, we think, for them to hope for.
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New York Yankees v Minnesota Twins
Trevor Plouffe of the Minnesota Twins misses a catch of a ball hit by... NyhetsfotoAmerican league,Baseboll - Sport,Basebollslag,Boll,Chris Parmelee,Fånga,Helkroppsbild,Match - Sport,Minneapolis,Minnesota,Minnesota Twins,Misslyckande,Ninth Inning,Robinson Cano,Sport,Target Field,Trevor Plouffe,USA,VertikalPhotographer Hannah FoslienCollection: Getty Images Sport 2013 Getty ImagesMINNEAPOLIS, MN - JULY 3: Trevor Plouffe #24 of the Minnesota Twins misses a catch of a ball hit by Chris Parmelee #27 of the Minnesota Twins during the ninth inning of the game on July 3, 2013 at Target Field in Minneapolis, Minnesota. The Yankees defeated the Twins 3-2. (Photo by Hannah Foslien/Getty Images)


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Educator Update
Friday, April 02, 2010
We want your feedback about your field trip experience! | Alie LeBeau
If you've been to the Aquarium on a field trip, we'd like your opinion about every aspect of the trip. From making the reservation and the check-in process to the interactive program with an Aquarium educator, we want to know what we did well and what we can do better.


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Ideal for small office machines and electronics. Feature EMI/RFI noise filtering, illuminated on/off switch and 15-amp circuit breaker. LED surge protection indicator and wiring verification.
Main Features
Comments about Fellowes Fellowes 6 Outlet Surge Suppressor:
A real diamond in the rough...not too many QUALITY 6 outlet surge supressors with a 15' cord out there. I've purchased well over 100 of these from Ritz, and not a single bad one in the bunch. Great product !!


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Oxford campus users only.
EconLit indexes scholarly journal articles, books, dissertations, and working papers throughout the field of Economics. Topics covered include economic development, forecasting, history, fiscal and monetary theory, business and public finance, healthcare economics, and international, regional, and urban economics
An artist's book is a work of art that takes the form of a book. But what does that mean? And what counts as a book, anyway? Is it a bunch of pages that have been bound together, or is there more to it than that?
These are the kinds of questions that artists' books try to answer. They play with both the form and the function of books as objects, encouraging onlookers to question traditional conceptualizations of "books." They are usually handmade, often unique, and all different from one another. Because most artist's books are hand-made and only crafted in small, limited runs, they offer artists a special opportunity to create books that are intensely personal.
All of the books featured in this exhibit are part of Miami University's Walter Havighurst Special Collections. The materials on display reflect a concentrated effort to build a diverse, provocative collection that will challenge students to reconsider their notion of "books" while also paying tribute to the creativity and innovation of book artists across the globe.
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Week 6 Spotify playlist: Staff recommendations
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Making a Book into a Video


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Wills-Kat to celebrate Prince George's first birthday with small, intimate party
Kate Middleton and Prince William will be throwing a birthday bash for their son Prince George on July 22, who turns one.
A spokesman for the palace told Us Magazine that the family is marking it privately with close family and friends at Kensington Palace.
Sources also said that the party will most likely be an indoor tea party that "may extend to the garden if the weather remains glorious."
The royal baby's great grandmother Queen Elizabeth, Uncle Prince Harry, and the Middleton family will be joining the party, while Prince Charles and Duchess Camilla will not be attending due to engagements in Scotland.
(Posted on 21-07-2014)
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