text:
April 13th, 2013 (F1plus).- It was said from the very beginning of the year that for Lotus to really aspire for a championship, either for constructors or drivers, the team would need to improve its qualifying, given the fact that race pace was among the best.
Well, it appears that this Saturday things clicked for the team with Kimi Raikkonen achieving second best time in the last round (Q3), which represents his first front row start since the 2009 Monaco Grand Prix.
The Finn used Pirelli's soft compound tyre and clocked a time of 1min 34.761 about a third slower than poleman, Lewis Hamilton.
"This is my best qualifying for the team which is good, but of course you always want to be on pole.
"It's been quite a tricky weekend so far and we've had a few small issues with setup, but it's always difficult to get things exactly how you want them and the car seems to be working well now.
"The tyres will of course be the question mark tomorrow; some people are starting on the primes but we chose the strategy we think will work best. Hopefully we can keep managing the degradation well like we have so far this season and have a good race tomorrow." Said Raikkonen.
As for Frenchman Romain Grosjean, he comfortably made it to the top ten with a Q3 time of 1min 35.364 good enough to place him sixth on the grid.
"Friday was difficult and we missed some time on Saturday morning so it's been a challenging weekend, but I'm finally back in business.
"We've been chasing some small problems here and there, but the engineers have been working hard and did a fantastic job to get the car to where it is now.
"My first lap on options in Q1 was actually my first of the weekend with those tyres so we had to guess a little on the set up, but I think we did pretty well. Of course you always want to do better, but overall I'm pleased. The car was much better this afternoon so let's see what we can do in the race." Said Grosjean.
Lotus said there's s strategy in place that obviously spins around the tyre choice and how they will evolve during the race. Having other drivers in the top ten with different compounds, initial stages of racing surely will be interesting.


text:
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Water, Coconut-base Surfactant (Decyl Glucoside), Vinegar, Grapefruit Seed Oil, Plant Essential Oils (Lavender, Clary Sage, Peppermint, Thyme, Sweet Marjoram). Ingredients are GRAS (Food-Grade).
Please read all label information carefully on delivery and prior to use.
Fantastik house hold cleaner is my go to cleaning product.I use this to remove junk of all kinds and it is effective. I also use to remove any kind of stain on my clothes.

subset: commoncrawl


text:
April 18, 2005
By James Jay Carafano, Ph.D.
This report is currently available only in PDF format.
Junking the policies justified by the Total Force Concept and theAbrams Doctrine may be a prerequisite for rethinking how theReserves are organized, employed, and resourced to increase thecapacity of citizen soldiers to respond rapidly to the wide rangeof emerging missions. The idea that force structure should serve asa presidential tripwire for the use of power should be abandoned.
James Jay Carafano, Ph.D.
Vice President for the Kathryn and Shelby Cullom Davis Institute for National Security and Foreign Policy, and the E. W. Richardson Fellow
Read More >>
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Our mission is to formulate and promote conservative public policies based on the principles of free enterprise, limited government, individual freedom, traditional American values, and a strong national defense. Read More
© 2015, The Heritage Foundation Conservative policy research since 1973


text:
Learing in Auroville
Reflections of a student intern
University of Washington - USA
I just wanted to learn how to sew. My original objective for my service commitment in Auroville was fairly modest. No attempt to change the world or to expand human consciousness. Just a simple desire to work with my hands. In my life up to my departure for India, I had always made it a point to do some sort of work that exercised a part of me other than my brain. At various times, that's taken on the form of being a jeweler, a janitor, a back waiter, or a gardener. It was work that kept me humble, that fed my soul, that served as a constant reminder that the awards and honors listed on my resume didn't really amount to much if writing essays was all I knew how to do. So as I planned my time in India, I wanted a similar kind of experience, one atypical yet typical of Leanne.
I remember reading the service learning description for Upasana Design Studio and being mildly interested but mostly amused. The language was so ridiculous to my American eyes, so fully representative of what I had imagined to be the extreme new-age hippiness of Auroville. I'm fairly certain I shared the description with a friend, accompanied by rolling eyes and the remark "Can you believe this?" All about the One and the Supreme and a myriad of other seemingly unnecessarily capitalized nouns. I was intrigued by the concept of design for social development, but had no idea what it meant and no intention of actually working for Upasana. So I settled on sewing at Lively Boutique instead, nice, simple, wholesome work that I could be proud of. Nothing flashy or grand; just honest and real.
And as seems to be the case so often in my life, I didn't get what I wanted but instead, got what I wanted but hadn't even known I wanted. I couldn't have identified as my original objectives what I've taken from Upasana because I had no idea that something this grand was even possible. My service learning experience has been on a level that I couldn't have understood or even imagined back in Seattle.
Upasana Design Studio appeared on my radar only after my service learning coordinator suggested it. After visiting the studio and sitting over tea with Uma for thirty minutes, I was sold. Uma is the heart and soul of Upasana, its founder and its keeper. I don't think it's an exaggeration to say that she is one of the most powerful woman I have ever met, terrifying and inspiring in the same sweep. I felt her energy, a pull to her vision and committed myself on the spot. On my first day, Uma showed me around the studio but refused to introduce me to anyone. She said that my relationships had to form organically. So she left me in the manufacturing room and told me to sit there and observe until I was ready to enter the space naturally. So I sat there awkwardly, trying to smile and make eye contact. Eventually, I made my way to the offices of the designers and pretty much never left.
My first few days were spent archiving textiles. The work was interesting enough but it didn't inspire my soul. I was ready to resign myself to such a fate, however, because I realized that to be daily surrounded by such rich and beautiful fabrics in India was a privilege and certainly nothing that I could ever experience in the same measure back at home. But then Uma approached me at the end of my first week -- after seeing that I had formed one or two organic relationships with the design team -- and told me she had a vision for me. I already knew all about Tsunamika, an enormously successful initiative to help hundreds of local fisherwomen devastated by the tsunami. But she said there was another project aimed at a completely different goal.
As I sat there watching the Small Steps PowerPoint presentation (one that I would eventually end up spending hours revising and re-envisioning), I could feel my heart beating with an intensity that made it difficult to breath. "This is it, this is it, this is exactly it," I kept thinking. When it finished, Uma looked at me with expectant eyes and said, "I'd like for you to coordinate the communications for our Small Steps project." I was in such disbelief at my enormous luck that I think I paused for a moment before I said, "Yes. Yes, yes. That's perfect."
So began a beautiful seven weeks devoted to an even more beautiful cause. It wasn't perfect. There were moments when I was less than the model worker, tempted to check my email instead of diligently serving the One. But then there were other moments when I was in absolute flow. When the words just poured from me. Times when I couldn't be peeled away from the keyboard, instances when I'd be revising a Small Steps poster or brochure and not even be unable to stop for the bathroom or tea until each phrase was exactly right, each word reflecting a true meaning.
As Communications Coordinator (a title I happily granted myself), I was in charge of understanding and internalizing the Small Steps vision, and then channeling that into a language that most people could understand. That meant polishing the power point presentation, crafting the text for and launching the website that can be accessed from anywhere, and writing a comprehensive project proposal that will be used to help grow a tree out of what is now only a seed. I also researched potential partners, drafted letters to other organizations, and spoke with visitors about the project.
My original intention had been to give my brain a rest, to let my hands work as my intellect reposed. But what I got was exactly the opposite. I spent my weeks alternating between chance ten-minute meetings with the super-busy Uma, followed by hours in front of the computer thinking deeply about what this project meant and how I could express it. As I had hoped, I wasn't toiling away in the tropical sun. But I was being challenged enormously. And challenged creatively. Pushed to see and work for a vision beyond me.
I had thought I would enter that studio with much to learn and little to give beside my time and energy. I was even a little anxious about how I would fit in with designers, alien creatures to me. But somehow, my energy and my skills wove seamlessly into theirs. Uma sat down with me on my last day and thanked me for what I had given to the project, telling me that I had added a "beautiful dimension" to the initiative in my ability to finally and fully articulate what the team had all known and felt but had struggled to find the words for. In my bio on the Upasana website, I shared a discovery I made:
My pen is my needle, my instrument for self-expression, my way of creating, manifesting and reflecting on beauty. I don't know how to sew, I've never designed a garment, but in the world of words, written or spoken, I am at home.
I realized that maybe I'm not all that different from designers. It's just that I design with words.
To say a few words about the actual project, "Small Steps is an initiative to promote a more environmentally and socially just world. The project's first step is to promote a responsible alternative to throw-away plastic bags. The Upasana team has designed a Small Steps cloth bag that we hope to distribute without cost throughout India, but our main aim is to promote the use of re-usable bags, whether or not they are ours." I have written this description so many times that it's become almost perfunctory. But its significance hasn't changed. I hesitate to say too much about the bags because the project isn't even about the bags. It's about cultivating a higher consciousness, recognizing the illogic of plastic bags and seeing our connection to a greater whole. If this sounds at all like anything we've been studying in class, it's because it is precisely what we've been studying in class.
So I guess my service learning experience in India ended up evolving into something fairly grand. It started out as a humble desire to learn to sew, and ended up as a fantastic attempt to change the world, to expand human consciousness, to 'contemplate the Divine' (the Sanskrit definition of 'upasana'). I suppose I succeeded at finding something that was unbelievably atypical and yet typical of Leanne. In other words, I got exactly what I wanted.
I end now with one more quote from myself. On my Upasana website bio, I was asked to articulate my design philosophy, an intimidating request but one I fulfilled with this:
As a perfectionist, the Upasana design motto resonates with me deeply, "Design for the Beloved." To act, work, and design always as if for those we love the most. I've never tried to articulate my own design philosophy, but if I had to, it would look very much like what I see happening here every day. Design as the channeling of a ceaseless love, creativity and energy.
|Auroville Universal Township


text:
Salon Edition 1" Tangle-free Hot Air Brush
We know you want the goods as soon as possible, which is why orders received by 3pm ET leave our warehouse the very same day. We'll send you an email that day to confirm shipment and the email will include your tracking information.
Our warehouse is closed Saturday & Sunday, so orders received after 3pm ET on Fridays will not ship out until Monday.
Salon Edition Hot Air Brush is a one inch rotating barrel that can create long lasting curls without an iron. This brush has soft bristles for tangle-free hair. It has a soft grip handle and a switch that locks in the barrel while curling and unlocks to unwind. The brush sleeve is removable for easy cleaning. The Salon Edition Hot Air Brush can style the hair as well as dry it quickly, eliminating the need of a blow-dryer. It's equipped with an eight feet swivel cord and a safety plug for shock protection.
- 250 watts of power for fast drying and styling performance, Rotating vented barrel releases tangle-free curls
- Locking barrel switch locks in place for curling and unlocks for unwinding, High / low heat control for all hair types
- Soft ball-tipped bristles gently grip hair, and rest softly against the head, Cool tip
- Removable brush sleeve for easy cleaning, 8-ft. tangle-proof swivel cord
- 8-ft. tangle-proof swivel cord, ProGuard Safety Plug with test and reset features
Folica stands behind the authenticity of everything we sell. Since 1998, Folica has made it our mission to provide you with an outstanding selection of hair and beauty products at highly competitive prices.
A new concept in hot air styling tools. Features gold thermal barrel that holds heat for faster styling and drying. Removable barrel for easy cleaning.New Price:
This quality brush iron has been designed and tested for performance, durability and handling ease.New Price:


text:
Analysis of Aldehyde Biomarkers of Exposure and Host Response
Recruitment status was Active, not recruiting
- Full Text View
- Tabular View
- No Study Results Posted
- Disclaimer
- How to Read a Study Record
Analysis of aldeyde biomarkers of exposure and host response The purpose of this prospective, open-label, clinical trial is to establish the feasibility and validity of exhaled breath condensate (EBC) biomarkers for use in studies designed to evaluate harm reduction strategies of smoking. This will be accomplished by measuring selected markers in EBC believed related to the pathogenesis of lung disease before and after a smoking cessation intervention.
Smoking Cessation
Drug: nicotine polacrilex
Drug: nicotine transdermal system
Drug: nicotine inhaler
|Study Type:
|Study Design:
|Intervention Model: Single Group Assignment
Masking: Open Label
Primary Purpose: Diagnostic
- Aldehyde biomarkers
|Study Start Date:
|March 2006
|Estimated Study Completion Date:
|July 2007
Twenty subjects will be accrued in three groups based on smoking habit: 10-20, 20-30 and >30 cigarettes/day. Eligibility criteria include male and female cigarette smokers ≥19 years of age, who are able to give informed consent, able to exhale into Eco Screen instrument for 15-20 minutes. All must be willing to make a serious quit attempt and be willing to use nicotine replacement therapy.
Subjects will be excluded if any of the following are present: regular use of anti-inflammatory medication; presence of any inflammatory disease of the respiratory tract including moderate or worse COPD (FEV1 < 80% predicted and FEV1/FVC ratio < 0.7). Subjects with normal lung function who meet criteria for diagnosis of chronic bronchitis will be excluded. Subjects with stable medical conditions, excluding inflammatory lung disease, will be permitted to participate, providing anti-inflammatory therapies are not used regularly and providing there has been no change in their clinical status in the two months prior to beginning the study.
Upon entering into the study, subjects will establish a quit date and will be treated with nicotine replacement therapy (NRT) products based on individual preference and will receive smoking cessation counseling. They will then be seen at 12 visits over the course of 25 weeks.
Evaluations will include vital signs, interval smoking history, food frequency questionnaire, exhaled carbon monoxide, blood drawn for chemistry and CBC, peripheral blood harvested and serum aliquoted and saved for future biochemical analysis, urine sample for isoprostane, pregnancy test for females, and exhaled breath condensate. Some visits may also include spirometry with and with out bronchodilators, 24 hour urine collections for NNAL and NNAL-glc quantification, health status assessed using St. George's Respiratory Questionnaire, the Functional Assessment of Chronic Illness Therapy – Fatigue, the Leicester Cough Questionnaire, the Clinical COPD Questionnaire and smoking related symptoms assessed using the Breathlessness, Cough, and Sputum Scale.
|Ages Eligible for Study:
|19 Years and older
|Genders Eligible for Study:
|Accepts Healthy Volunteers:
Inclusion Criteria:
- Smokers of at least 5 pack years;
- Stable smoking habit for 3 months;
- Willing to make a serious quit attempt;
- Able to use nicotine replacement therapy
Exclusion Criteria:
- Regular use of anti-inflammatory medication
- Inflammatory lung disease
- Unstable medical condition
Please refer to this study by its ClinicalTrials.gov identifier: NCT00482690
|Principal Investigator:
|Stephen I Rennard
|University of Nebraska
No publications provided
|ClinicalTrials.gov Identifier:
|NCT00482690 History of Changes
|Other Study ID Numbers:
|Study First Received:
|June 1, 2007
|Last Updated:
|June 4, 2007
|Health Authority:
|United States: unspecified
Keywords provided by University of Nebraska:
Smoking cessation
Exhaled breath
Harm Reduction
Cigarette smoking
Additional relevant MeSH terms:
Autonomic Agents
Cholinergic Agents
Cholinergic Agonists
Ganglionic Stimulants
Molecular Mechanisms of Pharmacological Action
Neurotransmitter Agents
Nicotinic Agonists
Peripheral Nervous System Agents
Pharmacologic Actions
Physiological Effects of Drugs
ClinicalTrials.gov processed this record on October 09, 2015


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Are You Afraid of the Dark: Campsites for the Fright Seekers
Looking for a vacation that will scare your socks off? There are plenty of great haunted spots across the country with amazing camping...
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Halloween is a time for fun traditions like pumpkin carving, haunted houses, hay rides and trick-or-treating. But did you know you can...
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Now that fall is here, temperatures turn crisp and cool in the northern states and the heat in the southern states starts to taper off,...
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James Cook was born in the village of Marton, Yorkshire on 27 October 1728 (7 November in the Gregorian calendar), one of five children of Scottish migrant farm labourer James Cook and his Yorkshire wife Grace. In August 1768 Cook set sail from Plymouth on his first voyage. Read more...
Captain James Cook
Features & facts
- Cook's North-West Passage expedition
- Cook narrative (PortCities)
- Cook fact file
- Hodges online - Art on Cook's second voyage
Cook's third voyage
Follow Cook's route on his 1776-78 voyage in search of the North-West passage.
A brief history of world exploration from Columbus to today.
Curator John McAleer talks about the life and death of Captain Cook. Part of the Museum's On the Line series of podcasts.


text:
1. MIRCHI MUSIC AWARDS 2012 2. On Location of Movie 'Mumbai 125 Kms' 3. Saif Ali Khan takes a bike ride to promote Agent Vinod
Added on 3/22/2012
By : wassupbollywood
Overall Rating :
Rate Me... Rating Saved!
View : 34902 Comments : 3 Video Courtesy : www.india-forums.com
|Total Votes : 7
|vaibhavjaveri, scorpio-girl, MahinK, KP_Marina, RoyalLEO_Krrish, Shwetaliciouss, MeriAashiqui.
karanvir bohra is married i think ... then howcome he is dating veena malik if to be believed rumours of zoomtv
Karanvir looks so good!! :)
Veena can't act that is for sure... will be watching for mah Superman Only! Best wishes for Mumbai-25km Karanvir. Btw IF how come Karanvir is not tagged in this VDO. Please tag him so that his fans can comment more.
Edited on:22 March 2012 at 9:58am


text:
Counties with fastest growing employment, March 2003
November 03, 2003
In March 2003, Placer County in California had the biggest over-the-year percentage increase in employment among the largest counties.
Employment in Placer County, California, located near Sacramento, grew by 4.9 percent from March 2002 to March 2003. It was followed by the counties of Lee, Florida, and St. Charles, Missouri (4.6 percent each), Rutherford, Tennessee (4.5 percent), and Pinellas, Florida (4.3 percent). In the nation as a whole, employment fell by 0.3 percent between March 2002 and March 2003.
The biggest absolute gains in over-the-year employment were recorded in the counties of Clark, Nevada (27,500), San Diego, California (20,900), Riverside, California (20,400), and Maricopa, Arizona, and Pinellas, Florida, (18,500 each).
The BLS Quarterly Census of Employment and Wages program produced these data. Pay data presented here are for all workers covered by State and Federal unemployment insurance programs. The largest counties are the 315 U.S. counties with employment levels of 75,000 or more. Find more about pay and employment in large counties in "County Employment and Wages: First Quarter 2003" (TXT) (PDF), news release USDL 03–654.
Bureau of Labor Statistics, U.S. Department of Labor, The Economics Daily, Counties with fastest growing employment, March 2003 on the Internet at http://www.bls.gov/opub/ted/2003/nov/wk1/art01.htm (visited October 05, 2015).
Recent editions of Spotlight on Statistics
New estimates of personal taxes in Consumer Expenditure Survey
In 2013, the Consumer Expenditure Survey improved its personal tax data.
Trends in long-term unemployment
Long-term unemployment reached historically high levels following the recession of 2007–2009.
Housing: before, during, and after the Great Recession
looks at consumer expenditures on household items, employment in residential construction, prices for household items, and injuries in occupations involved in building and maintaining our homes.


text:
Located in the community of El Segundo, the comfortable Embassy Suites Los Angeles – International Airport/South hotel is less than 2 miles from LAX. Discover some of the area's best beaches and most popular attractions from our full-service hotel. Within easy reach of the headquarters of several Fortune 500 companies, the Port of LA and LA Air Force Base, this Los Angeles, CA all-suite hotel offers flexibility and value whether you are staying for a few nights or a few weeks Remain productive during your stay thanks to the 24-hour BusinessLink™ business center and high-speed internet access. Your two-room suite gives you the space to work or relax, while the microwave and refrigerator allow you to prepare delicious snacks from the comfort of your suite. Work out in the fully equipped Embassy Suites Fitness Center or swim laps in the indoor pool. Start your day in style with a complimentary cooked-to-order breakfast, then unwind with drinks and snacks in the evening at the complimentary Evening Reception*. The hotel's 1440 bistro & bar takes a contemporary and sophisticated approach to classic American cuisine * Service of alcohol subject to state and local laws. Must be of legal drinking ageHilton
24 hour front desk
Free airport shuttle
ATM on-site
Business centre
Complimentary breakfast
Concierge service
Cribs available
Express check out
Fitness centre
Gift shop
Hot tub
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Indoor pool
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Pets not allowed
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text:
Changes related to "Opium"
From Uncyclopedia, the content-free encyclopedia
← Opium
This is a list of changes made recently to pages linked from a specified page (or to members of a specified category). Pages on your watchlist are bold.
October 13, 2015
|m 14:33
|Kitten Huffing (diff | hist) . . (-3) . . Philologia (talk | contribs) (→The War on Kitten Huffing: apostrophes (children's, PSAs & 70s - the last 2 are simple plurals, not contractions or, obviously, possessives))


text:
The Naked And Famous - Leeds Festival 2011 review
'Only a mid-set lull stops this from being a true weekend highlight'
Photographer: Trevor EalesGraeme Johnston on 27 August 2011
Someone obviously forgot to tell The Naked And Famous that today is the ugly side of the Leeds bill.
While Bring Me The Horizon blast out their aggressive, nasty metal on the main stage, the Radio 1/NME tent is home to a chilled, elegant set that packs a touch of straight up rock n roll with the glittering indie electro.
That a band as chock full of the sort of gorgeous, massive pop melodies that shine through on opening efforts 'Passive Me, Aggressive You' and 'Punching In A Dream' aren't playing the biggest stage to a bigger crowd is sinful. It is a matter of when, not if for New Zealand's finest export.
Only a mid-set lull of more tame, less catchy numbers stop this from being a true highlight of the weekend.
However, when they are good, they sound destined to knock Adele and the other overrated mopes from the top of the charts, as demonstrated on the colossal and anthemic climax, 'Young Blood'. Stunning.
Click for more Reading and Leeds 2011 reviews.
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Follow us on Facebook and Twitter and reply to our review posts with your thoughts, insights, opinions and full-on reviews.
Send tweets to us with your reviews or comment on our Reading and Leeds Facebook posts or in the comments section below with your thoughts.
This isn't about getting nasty if you don't agree with our journalists, but an opportunity to leave your own appraisals, with the best ones getting published on the site.
So, what are you waiting for? Let us know your thoughts!


text:
T-Mobile Galaxy Note 4 pre-orders open on September 24, available to buy Oct. 17
T-Mobile today announced that it will be opening up pre-orders for the next hotly-anticipated Samsung device: The Galaxy Note 4. The device will be available to pre-order from September 24th, with the release scheduled for October 17. Price-wise, you're looking at a device priced the same as the base-model iPhone 6 Plus: $749. On EIP, that's $0 down with 24 monthly payments of $31.24. Pre-registration for the device has been very popular, as … [read full article]


text:
- Filed Under
OK, so the rollout of the health insurance marketplaces created under the Affordable Care Act didn't go so smoothly.
Technical glitches and heavy traffic occurred almost right away. Nonetheless, interest in the exchanges hasn't waned. As open enrollment continues, many people will have questions about the marketplaces. Here are some queries I've received from readers.
Question: I have two granddaughters, 20 and 21. One is working part time for a small business and the other is a student. Neither currently has health insurance. If they enroll on the exchange by Dec. 15 and at some future ...


text:
|Select the Course Number to get detailed information on the course. Select the desired Schedule Type to view the sections listed for the course with that Schedule Type. If your selection returns no values, refer to the Help for more information.
|ENG 331 - ECCE:Asian American Women
This course explores the complex nature of Asian-American literature to discover its diverse themes, foci, narrative strategies, and approaches to culture and history, with a particular focus on women. Course Information: Same as WGS 331. This course fulfills an Engaged Citizenship Common Experience requirement at UIS in the areas of Global Awareness.
4.000 Credit hours
Levels: Undergrad - Springfield
Schedule Types: Lecture-Discussion, Online
English and Modern Languages Department
Course Attributes:
UIS: Global Awareness (ECCE)


text:
Pescado en la dieta
El pescado es una gran alternativa a las carnes rojas, ya que es una proteína magra y saludable y que contiene un tipo de grasa llamada "omega 3" que puede ayudar a proteger el corazón.
Last reviewed 5/24/2011 by David C. Dugdale, III, MD, Professor of Medicine, Division of General Medicine, Department of Medicine, University of Washington School of Medicine. Also reviewed by David Zieve, MD, MHA, Medical Director, A.D.A.M. Health Solutions, Ebix, Inc.
- The information provided herein should not be used during any medical emergency or for the diagnosis or treatment of any medical condition.
- A licensed medical professional should be consulted for diagnosis and treatment of any and all medical conditions.
- Call 911 for all medical emergencies.
- Links to other sites are provided for information only -- they do not constitute endorsements of those other sites.
©1997 - A.D.A.M., Inc.Any duplication or distribution of the information contained herein is strictly prohibited.


text:
Are Farm Subsidies Making Us Fat?
Who Will be The Biggest Farm Bill Loser?
It is often argued that subsidies have lead to the overproduction of commodity crops like corn and soybeans, creating cheap junk food that makes us fat. The farmers lose and so do consumers. Watch this video to learn more…
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A letter from our friends at Food & Water Watch:
A new episode of America's favorite weight-loss reality show, The Biggest Loser, airs tonight and we're reminded, once again, that we all struggle to maintain healthy lifestyles with good, whole foods and exercise. But how much of the problem is our own and how much of it is the result of bad farm policy?
Because you're one of our valued supporters, we want you to be the first to see our "reality show" debut: The Biggest Farm Bill Loser!
It is often argued that subsidies have lead to the overproduction of commodity crops like corn and soybeans, creating cheap junk food that makes us fat. This seems like a logical argument but it is grossly oversimplified. When you take a closer look, we find that the deregulation of commodity markets — not subsidies — has driven down the price of these commodity crops. Deregulation has made big corporations rich while farmers have struggled to survive. Based on this, can you guess who The Biggest Farm Bill Loser will be?
This is not an easy issue to understand so we put together this animated video to help. It is vital that we share this video to help educate and empower our fellow consumers and farmers to speak out for real change in our food system. Watch the video, share it with friends, post it on your social networks, spread the word!
Thanks for taking action,
Meredith Begin
Education & Outreach Organizer
Food & Water Watch
Pig Photo Credit: Sarah209
Originally published on I Count for myEARTH.
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4 Responses to "Are Farm Subsidies Making Us Fat?"
From an economic standpoint, subsidies are bad because they skew the markets. When you subsidize one particular company or product, it creates an illusion that those are more efficient applications of your resources (and money). Subsidies are often applied for limited time periods and purposes, such as jumpstarting a new industry (i.e. alternative energy). The truth, however, is that once in place, subsidies are almost impossible to eliminate, because you have to "take" something away from someone.
While some subsidies are readily apparent, there are far more that are hidden. Government programs support industries in many ways, such as education programs, marketing assistance, subsidized fuel, and academic/scientific assistance. Still, being aware that these subsidies exist is a start. Buying local is sometimes helpful, although subsidies exist there, as well. Conscious consumerism is the key, keeping in mind that everything is, as the Buddhists say, is interdependent.
She looks… too old. Something's off. I don't see it. That wig is AWFUL.Report this comment as spam or abuse


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Taylor Hart moving to the edge
Tualatin's Taylor Hart was one of two Ducks to start all 14-games on the defensive line in 2011. Hart makes the fulltime move from DT to DE for his junior season.
Growing up around his grandfathers lumberyard has helped #66 know what hard work is.
Hear from Taylor Hart in this KVAL Sports video.


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Death Toll In Kenyan Siege Expected To Rise
A morgue worker attends to bodies of the Westgate Mall attack at... News PhotoAttending,Conflict,Dead Person,Funeral Parlor,Horizontal,Human Interest,Kenya,Killing,Mass Murder,Morgue,Nairobi,Nairobi Mall Shooting,Occupation,Terrorism,Violence,War,Westgate Shopping Mall - NairobiPhotographer SIMON MAINACollection: AFP 2013 AFPA morgue worker attends to bodies of the Westgate Mall attack at September 25, 2013 at Nairobi's mortuary. Kenyan troops and rescue workers scoured the wreckage of a Nairobi shopping mall on September 25, 2013 for bodies and booby-trapped explosives after a four-day siege by Islamist gunmen left 67 dead and dozens more missing. AFP PHOTO / SIMON MAINA (Photo credit should read SIMON MAINA/AFP/Getty Images)


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The definition of a hypothetical protein is a protein that is predicted to be expressed from an open reading frame, but for which there is no experimental evidence of translation. Hypothetical proteins constitute a substantial fraction of proteomes of human as well as of other eukaryotes. With the general belief that the majority of hypothetical proteins are the product of pseudogenes, it is essential to have a tool with the ability of pinpointing the minority of hypothetical proteins with a high probability of being expressed.
Here, we present an in silico selection strategy where eukaryotic hypothetical proteins are sorted according to two criteria that can be reliably identified in silico: the presence of subcellular targeting signals and presence of characterized protein domains. To validate the selection strategy we applied it on a database of human hypothetical proteins dating to 2006 and compared the proteins predicted to be expressed by our selecting strategy, with their status in 2008. For the comparison we focused on mitochondrial proteins, since considerable amounts of research have focused on this field in between 2006 and 2008. Therefore, many proteins, defined as hypothetical in 2006, have later been characterized as mitochondrial.
Among the total amount of human proteins hypothetical in 2006, 21% have later been experimentally characterized and 6% of those have been shown to have a role in a mitochondrial context. In contrast, among the selected hypothetical proteins from the 2006 dataset, predicted by our strategy to have a mitochondrial role, 53-62% have later been experimentally characterized, and 85% of these have actually been assigned a role in mitochondria by 2008.
Therefore our in silico selection strategy can be used to select the most promising candidates for subsequent in vitro and in vivo analyses.
According to the Human Genome Organization (HUGO), the human genome is predicted to consist of 19599 protein-encoding genes [[1], Human Genome Project http://www.hugo-international.org/ webcite]. A substantial part of these genes is predicted to encode a group of proteins, where translation has not been demonstrated and the proteins themselves have not been characterized. This group of proteins is accordingly defined as hypothetical. Although many of the listed hypothetical proteins most likely are predicted products of pseudogenes, there is a reasonable probability that a number of the listed hypothetical proteins are truly novel and can perform uncharacterized biological functions. Consequently, the putative importance of hypothetical proteins is not negligible.
Several in silico methods are available for descriptive predictions of proteins with unknown function. These include studies of homology, database searches for orthologs, or the presence of characterized functional domains or motifs within the protein [2]. Most often false positives will occur and predictions must be substantiated by in vitro and/or in vivo experiments to validate and further characterize predicted functionality. The in silico methods are designed for functional prediction of a protein, but not specifically designed to ascertain whether a protein is hypothetical or not. When screening hypothetical proteins for novel translatable candidates, in silico methods are therefore rarely used and the researcher often performs the screen with laborious in vitro and/or in vivo experiments.
In the present study, we propose an in silico screening strategy for eukaryotic systems, in which novel translatable candidates can be selected from a group of hypothetical proteins. The strategy is based on in silico methods normally used to make functional predictions of proteins, which include search for presence of sub-cellular targeting signals and for presence of characterized protein domains. Especially targeting signals and, to a lesser extent, protein domains can be predicted with high probability. The occurrence of either targeting signals or identifiable protein domains can also be present in pseudogenes as a result of gene duplication. However, we hypothesize that the risk of a hypothetical protein being a pseudogene is greatly reduced when both targeting signals and protein domains are identified in the transcript, especially if the protein domain architecture suggests a relevant function in the predicted sub-cellular compartment. Selection of hypothetical proteins based on a combination of both these factors should therefore greatly increase the success rate of discovering true functional proteins with roles in subcellular compartments among hypothetical proteins. Due to the design of the selection strategy it is ineffective for identifying proteins without localization signals, and this must be taken into consideration.
To exemplify our selection strategy we have chosen mitochondria as the targeted sub-cellular compartment. Within recent years, a substantial amount of work has been invested in compiling a near complete list of mitochondrial proteins in humans. This has resulted in the establishment of the MitoCarta database http://www.broad.mit.edu/pubs/MitoCarta/ webcite[3]. The total number of genes encoding mitochondrial proteins is according to MitoCarta at least 1013 [3]. Mitochondria are semiautonomous organelles present in almost all eukaryotic cells ranging from a single copy to several thousands. Mitochondria contain their own autonomous genome, which encodes 37 of these proteins. The remainder is encoded by nuclear DNA and imported into mitochondria. Examples of mitochondrial functions include ATP production by oxidative phosphorylation, β-oxidation of fatty acids, metabolism of amino acids and of lipids. Furthermore, mitochondria have a prominent role in apoptosis.
With the exception of proteins encoded by the mitochondrial genome, proteins are translated in the cytosol from their corresponding mRNA. Many proteins are transported to specific parts of the cell where they function in context of the sub-cellular compartment. The sub-cellular localization of proteins can be facilitated by specific targeting peptides. There are two types of targeting peptides, the presequences and the internal targeting signals. Presequences are often localized at the N-terminal whereas internal targeting signals can be distributed throughout the protein sequence [4-6]. The mitochondrial membrane contains translocases for recognition and import of nuclear-encoded mitochondrial proteins. The translocase of the outer mitochondrial membrane (TOM complex) is responsible for recognition and initial import of nuclear-encoded mitochondrial proteins (reviewed in [7]). Mitochondrial precursor proteins posses either an N-terminal presequence or internal targeting signals. Both types of targeting peptides, N-terminal or internal, are recognized by different import receptors of the TOM complex. N-terminal presequences generally have a length of 6-85 amino acid residues, enriched in Arg, Ser and Ala, while negatively charged amino acids are rarely present [8]. N-terminal presequences form positively charged amphiphilic α-helices when bound to import receptors on the mitochondrial surface [9], and upon mitochondrial import, presequences are removed by proteolysis (reviewed in [10,11]). Even though binding of different parts of the TOM complex to varying internal targeting signals has been shown [12], a common motif for an internal targeting signal still has to be elucidated,
In order to validate that our in silico selection strategy can predict functional candidates among hypothetical proteins we chose to focus on proteins with a predicted mitochondrial function. We have utilized an existing database of hypothetical proteins assembled in 2006 [13]. From this database we selected all hypothetical proteins predicted to be localized in human mitochondria due to the presence of a putative mitochondrial N-terminal presequence. These selected proteins were then investigated for the presence of potentially functional protein domains. We predict that the sub-group of hypothetical proteins, with both a mitochondrial N-terminal presequence and potentially functional protein domains has a high probability of being expressed and of having a function in a mitochondrial context.
All proteins investigated were hypothetical in 2006. However, between 2006 and 2008, many proteins have been experimentally characterized or removed from the database as they have been proven to be products of pseudogenes. This increases the probability that the 2006 dataset of hypothetical proteins includes a large number of proteins that are now (as of 2008) classified as mitochondrial. By applying the selection strategy on the 2006 dataset, we are able to compare the resulting predictions with the factual in vitro and/or in vivo characterizations of the proteins performed from 2006 to 2008. Effectiveness of the selection strategy can be demonstrated by comparing proteins selected from the 2006 dataset with the number of these proteins that, as of November 2008, are demonstrated to be translated, mitochondrial or proven to be pseudogenes.
We have utilized a database of proteins extracted from GenBank in August 2006. At the time of extraction, all proteins were defined as hypothetical and all sequences were crosschecked and annotated [13]. In November 2008, the status of each individual protein was reinvestigated and entries of the 2006 database that later have been identified as duplicates were removed. The entries of the 2006 dataset were divided into three groups according to their individual status in November 2008: Hypothetical proteins, characterized proteins and proteins discovered to be pseudogenes and therefore removed by GenBank. These three groups are in the following collectively referred to as the 2008 dataset.
Several prediction programs have been designed to predict the localization of eukaryotic proteins. In table 1, we have listed a selection of available programs, which have been reported to have a medium to high prediction accuracy [14-22]. To exemplify the occurrence of hypothetical proteins with functional targeting peptides, hypothetical proteins from the 2006 dataset were analyzed using pTarget. The pTarget program http://bioapps.rit.albany.edu/pTARGET/ webcite predicts protein targeting to nine different sub-cellular locations including mitochondria. Prediction is based on the occurrence of specific Pfam domains earlier determined to be location specific. pTarget, can predict 68-87% of the true positives at accuracy rates of 96-99% [19,20].
Table 1. Overview of subcellular localization prediction programs for eukaryotic proteins
In contrast to pTarget, the TargetP program http://www.cbs.dtu.dk/services/TargetP/ webcite predicts mitochondrial localization using the N-terminal sequence information only, with a success rate of predictions of 90% [21]. TargetP was used to screen the 2006 dataset for functional mitochondrial targeting peptides.
All hypothetical proteins predicted to have a mitochondrial targeting peptide by TargetP, were further characterized using the SMART program http://smart.embl-heidelberg.de/ webcite. The SMART program identifies protein domains from a database of manually annotated known protein domains [23,24].
We hypothesize that hypothetical proteins, predicted to contain both a mitochondrial N-terminal presequence and functional protein domains have a high probability of being functional in a mitochondrial context. To verify our hypothesis, we used the 2006 dataset of the, then, hypothetical proteins. Using TargetP, we selected proteins having a high probability of containing a mitochondrial N-terminal presequence. For the resulting subset of proteins we used SMART to search for the presence of functional protein domains. Comparing with protein status according to the 2008 dataset, we determined the percentage of selected proteins that had either been removed or experimentally characterized after 2006. Furthermore, if proteins had been experimentally characterized, we determined if they had been found to be functional in a mitochondrial context.
To demonstrate that the effectiveness of our in silico selection strategy is not dependent on neither TargetP nor the SMART program, the localization prediction programs, MITOPRED http://bioapps.rit.albany.edu/MITOPRED/ webcite[16] and WoLF PSORT http://wolfpsort.org/ webcite[22] were used in conjunction with the SMART program, to screen the 2006 dataset for proteins predicted to be mitochondrial. MITOPRED and WoLF PSORT have been reported to have high prediction accuracy of proteins that are localized to the mitochondria. Prediction of localization is based on the occurrence of Pfam domains and known sorting motifs rather than the presence of mitochondrial presequences as basis for prediction of protein targeting [16,22]. The effectiveness of the selection strategy using MITOPRED or WoLF PSORT was compared to the effectiveness of the selecting strategy using TargetP. Correspondingly, the effectiveness of our selection strategy was investigated, when the SMART program was replaced with the Prosite scanning tool http://www.expasy.ch/prosite/ webcite[25]. Like the SMART program, Prosite identifies protein domains from a database of manually annotated known protein domains.
Results and discussion
After removing proteins found or predicted to be duplicates of already existing proteins, the 2006 dataset of hypothetical proteins contains 5860 proteins. According to GenBank's current annotation (November 2008) of the same group of proteins, 1455 of the 5860 proteins annotated as hypothetical in 2006, are still hypothetical, while 1215 proteins have been experimentally characterized and 3190 proteins have been removed by GenBank as they have been identified as pseudogenes (See Additional file 1).
Additional file 1. 2006 & 2008 Dataset. Protein entries of the 2006 and 2008 dataset. Results from our in silico selection strategy.
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This file can be viewed with: Microsoft Excel Viewer
pTarget was used to predict the distribution of human hypothetical proteins from the 2006 dataset and the 2008 dataset (Table 2). pTarget is used to exemplify how human hypothetical proteins can be sorted based on their predicted cellular localization. This is important since our selection strategy is limited to proteins targeted for a sub-cellular localization. Using pTarget as an indicator only, we are able to demonstrate that proteins predicted to be localized to lysosomes, golgi, peroxysomes, mitochondria or endoplasmic reticulum, comprise of 32% of the 2006 dataset. When including proteins predicted to be secreted, proteins targeted for the plasma membrane or nucleus, these include 87% of the 2006 dataset. This indicates that the selection strategy, according to pTarget, can be applied on up to 87% of the dataset.
Table 2. Predicted subcellular distribution of human hypothetical proteins
TargetP predicts probability of mitochondrial localization based solely on mitochondrial specific presequences. These motifs do not necessarily require cis or trans acting domains in order to be fully functional mitochondrial targeting signals. Accordingly, if a hypothetical protein is predicted to be localized to the mitochondria, there is a reasonable probability that a corresponding, expressed protein would be localized to this organelle even though it may still be the product of a pseudogene. Screening the 2006 dataset of human hypothetical proteins with TargetP we found a total of 1139 proteins predicted to be localized to mitochondria (See Additional file 1). TargetP places all of the predicted proteins into reliability classes, ranging from A to E, where A indicates the strongest prediction [21]. We have focused on the total of 538 proteins belonging to reliability class A (52 proteins), B (204 proteins) and C (282 proteins) (See Additional file 1).
From reliability class A to C, 315 of 538 proteins (59%) selected by TargetP, have been removed, while 75 of 538 proteins (14%) have been characterized and 32 of 538 proteins (6%) have been characterized as mitochondrial. When focusing on proteins predicted by TargetP to be in reliability class A, 15 of 52 proteins (29%) have been removed, 18 of 52 proteins (35%) have been characterized and 14 of 52 proteins (27%) have been characterized as mitochondrial (See Additional file 1). From the 2006 dataset of hypothetical proteins, 67 are listed in MitoCarta as characterized mitochondrial proteins (See Additional file 1). TargetP was successful in identifying 32 of the total of 67 mitochondrial proteins, but did also select 315 proteins that after 2006 have been removed by GenBank. When focusing on proteins belonging to reliability class A, 14 of 67 mitochondrial proteins were identified, while 15 proteins have been removed. This demonstrates that TargetP is efficient in finding mitochondrial proteins, but it is not suitable for screening hypothetical proteins for novel translatable candidates. To be able to annotate the selected hypothetical proteins we screened proteins from reliability class A to C with the SMART program to determine the presence of known protein domains. We ignored proteins only containing transmembrane domains, coiled coil regions, signal peptides and/or segments of low compositional complexity, as these regions are not unique protein domains.
Three groups of proteins were constructed from the 538 proteins investigated. Group I consists of 20 proteins, predicted by TargetP to belong to reliability class A and to contain identifiable protein domains according to SMART prediction. Group II consists of 56 proteins, predicted by TargetP to belong to reliability classes A and B and to contain identifiable protein domains. Group III contains 100 proteins that are predicted by TargetP to belong to reliability classes A, B and C and to contain identifiable protein domains. Group III therefore contains all proteins of group I + II and group II contains all proteins of group I. The construction of these three groups allows us to comment on how reliable the prediction of cellular localization should be in order to get a good result from our selection strategy.
The three groups of selected proteins were, together with the 5860 hypothetical proteins from the 2006 dataset, compared with their corresponding 2008 annotations. The comparison includes number of experimentally characterized proteins, number of experimentally characterized mitochondrial proteins and number of proteins removed due to being the predicted result of pseudogenes or due to having similarity to an existing protein (Table 3).
Table 3. Comparison of predicted versus experimentally determined status of proteins
25% of the 5860 proteins were in November 2008 still annotated as hypothetical, 21% had been experimentally characterized and 54% have been removed. Of the experimentally characterized proteins, 67 proteins or 6% were listed in MitoCarta as mitochondrial. Group III contains 100 proteins, where 36%, as of November 2008, are still hypothetical, 53% have been characterized and 11% have been removed. Of the characterized proteins 45% or 24 proteins are listed in MitoCarta as mitochondrial. The values obtained clearly demonstrate, that our strategy using a selection based both on the presence of a mitochondrial presequence and identifiable protein domains is very efficient for extracting hypothetical proteins with a functional role in mitochondria. Applying the selection strategy on the 2006 dataset identified 24 of 67 proteins that later have been categorized as mitochondrial. Furthermore the percentage of removed proteins is 5-fold lower for group III when compared with the 2006 dataset and the percentage of characterized proteins is concurrently 2.5 fold higher. When investigating group II and group I proteins, selected by our strategy it is evident that the percentage of removed proteins is diminished to 9% and 5% respectively, and the percentage of characterized proteins is increased to 65% and 64% respectively. The prevalence of mitochondrial proteins within the number of characterized proteins increases from 6% for the total 2006 dataset to 45%, 58% and 85% for group III, group II and group I respectively.
When increasing the selectivity of the applied prediction models, in our case by focusing on group II and especially group I proteins, it is evident that the probability of finding hypothetical proteins, which will have a function in the predicted sub-cellular compartment is increased. However, it is also evident that the higher the selectivity, the lower the total number of identified proteins with the desired functionality. For group III proteins, 24 out of a total of 67 mitochondrial proteins were discovered. For group I, only 11 proteins out of the 67 mitochondrial proteins were discovered.
The three groups of investigated proteins contain a total of 36 proteins that in November 2008 was still annotated as hypothetical. According to our selection strategy, these proteins are predicted to be expressed and to have roles in a mitochondrial context. To investigate the potential of these proteins, we investigated the protein domains of 6 hypothetical proteins of group I to see if they would suggest a mitochondrial function for the relevant protein (Table 4). 4 out of the 6 proteins each contain one domain that are experimentally characterized in mitochondria and therefore may have putative mitochondrial functions. The four domains were found to be a Complex I-Lyr domain, a Methyltransferase 12 domain, a Sel 1 domain and a DUF1640 domain. The Complex I-LYR domain is present in a family of proteins including the mitochondrial NADH-ubiquinone oxidoreductase complex I. The methyltransferase 12 domain is found in a variety of methyltransferases including one functioning in mitochondria. Sel I like repeats domain is found in a vast amount of proteins including HSP70, HSP90, and in the mitochondrial Tom 70 import receptor. The DUF1640 domain is present in the mitochondrial protein FMP32 found in Saccharomyces cerevisiae. [26-30]
Table 4. Identified protein domains of 6 hypothetical proteins
To demonstrate that the effectiveness of our in vitro selecting strategy is not dependent on neither TargetP nor the SMART program, MITOPRED and WoLF PSORT replaced TargetP and was together with the SMART program, used as basis for our selection strategy in order to screen the 2006 dataset for proteins predicted to be mitochondrial. Similarly, the SMART program was replaced by Prosite and was together with TargetP used as basis for our selection strategy in order to screen a selection of the 2006 dataset (See Additional file 1). Using MITOPRED to screen the 2006 dataset, 807 out of 5860 proteins were predicted to be mitochondrial. Of the 807 proteins, 394 (49%) have been removed, 164 (20%) have been characterized and 35 of these (21%) have been characterized as mitochondrial. Using a combination of MITOPRED and the SMART program, 198 out of 5860 proteins were predicted to be mitochondrial. Of the 198 proteins, 16 (8%) have been removed, 113 (57%) have been characterized and of these 28 (25%) have been characterized as mitochondrial (Table 5).
Table 5. Validation of selection strategy using a variety of prediction tools
Using WoLF PSORT to screen the 2006 dataset, 754 out of 5860 proteins were predicted to be mitochondrial. Of the 754 proteins, 199 (26%) have been removed, 123 (16%) have been characterized and 31 (25%) of these have been characterized as mitochondrial. Using a combination of WoLF PSORT and the SMART program, 154 out of 5860 proteins were predicted to be mitochondrial. Of the 154 proteins, 11 (7%) have been removed, 94 (61%) have been characterized and 26 of these (28%) have been characterized as mitochondrial (Table 5). Using MITOPRED or WoLF PSORT alone to screen the 2006 dataset, respectively 35 and 31 of the total of 67 mitochondrial proteins were identified. However, both MITOPRED and WoLF PSORT did also select 394 proteins that after 2006 have been removed by GenBank. As was the case with TargetP, MITOPRED and WoLF PSORT are efficient in finding mitochondrial proteins, but they are not alone suitable for screening hypothetical proteins for novel translatable candidates. By replacing TargetP with either MITOPRED or WoLF PSORT, our selection strategy is able to identify 26 to 31 of the total number of 67 proteins listed in MitoCarta as characterized mitochondrial proteins. Only 7-8% of the proteins have been removed and the remainder is either characterized or still hypothetical. This demonstrates the efficiency of our in silico selection strategy using MITOPRED or WoLF PSORT is comparable to our selection strategy using TargetP.
Of the proteins identified by our selection strategy based on TargetP, up to 85% of the proteins that have been characterized have been characterized as mitochondrial. For our selection strategy based on MITOPRED or WoLF PSORT, the corresponding values are 25% and 28% respectively. The specificity of our selection strategy is therefore dependent on the method of localization prediction used. TargetP relies on the presence of well-defined mitochondrial presequences, whereas MITOPRED and WoLF PSORT rely on putative Pfam domains and signaling motifs.
The SMART program was replaced with the Prosite prediction tool, which together with TargetP was used in our selection strategy to analyze a subset of the 2006 dataset. TargetP predicted 52 of the 5860 proteins to belong to reliability class A. 15 of the 52 proteins have been removed and 14 of the 52 proteins have been characterized as mitochondrial. Only 9 of the 52 proteins were by Prosite found to contain known protein domains. 1 of the 9 proteins is still annotated as being hypothetical, while 8 of the found proteins have been characterized as mitochondrial. Prosite was therefore able to identify 8 of the 14 mitochondrial proteins present in the subsection of the 2006 dataset predicted by TargetP to be in reliability class A and furthermore, none of the removed proteins were selected by Prosite. In comparison, the SMART program was able to identify 11 of the 14 mitochondrial proteins. For the subset of proteins investigated, the efficiency of our selection strategy base on Prosite is therefore comparable with our selection strategy based on the SMART program.
A hypothetical protein may have a, yet uncharacterized, role in a biological context or simply be the predicted result of a pseudogene and with no biological relevance. In order to screen a dataset of hypothetical proteins, we propose a simple selection strategy where proteins are selected on the basis of well-characterized targeting peptides and protein domains. We have utilized a database of hypothetical proteins dating from 2006 and reviewed their annotated status in 2008. Accordingly, we can verify our selection strategy by reviewing the proteins that were hypothetical in 2006, but have been experimentally characterized by November 2008. We chose to screen for hypothetical proteins predicted to be mitochondrial since considerable amounts of work have been performed within the last couple of years to build extensive databases of the human mitochondrial proteome, summarized in works like MitoCarta. From the 2006 dataset, 5860 hypothetical proteins were identified, and from this dataset, we used TargetP together with the SMART program to identify 100 proteins that we believe, have a high probability of being expressed mitochondrial proteins, based on our selection strategy. This list is not exhaustive as, for instance, hypothetical proteins with mitochondrial internal targeting signals but no mitochondrial presequence, were not considered. When compared to the 2008 dataset, we found that 53 of the 100 hypothetical proteins predicted to be mitochondrial have now been characterized, and 45% of those were found to be mitochondrial. In comparison, only 6% of the characterized proteins from the 2006 dataset have been categorized as mitochondrial in the same time period. Increasing the selectivity of TargetP increases the incidence of characterized mitochondrial proteins to 85%, but unfortunately decreases the total number of mitochondrial proteins identified.
Investigating identified protein domains of 6 of the 36 hypothetical proteins predicted to be mitochondrial, we found a subset of 4 proteins having a strong mitochondrial signature in their identified protein domains. It is our opinion that these proteins are very interesting candidates for further experimental characterizations.
In present work we have applied our selection strategy in the search for human mitochondrial proteins using TargetP and the SMART program. From the characterized proteins we were able to verify the fidelity of our in silico selection strategy.
By using different combinations of prediction tools, we demonstrated that our selection strategy is general. The possibility of selecting different prediction tools thereby allows the identification of hypothetical proteins with a high probability of having a role in any organelle compartment where the internal targeting signals are characterized. Furthermore, many of the targeting signals and identifiable protein domains that are valid in human cells, are also valid in other eukaryotes. Our selection strategy can therefore be applied on a wide array of organisms.
Authors' contributions
CD and PS contributed the initial concept for this work. CD, PS, MS, MR, and LJR participated in the design of the study and performed the analyses. Paper was written with insights from the other authors. All authors read and approved the final manuscript.
We thank Sofie Dalbros Andersen for suggestions and critical reading of the manuscript. This work was supported by The Danish Cancer Society (LJR).
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Schultz J, Milpetz F, Bork P, Ponting CP: SMART, a simple modular architecture research tool: identification of signaling domains.
Proc Natl Acad Sci USA 1998, 95(11):5857-64. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Letunic I, Copley RR, Pils B, Pinkert S, Schultz J, Bork P: SMART 5: domains in the context of genomes and networks.
Nucleic Acids Res 2006, 34:D257-60. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
de Castro E, Sigrist CJ, Gattiker A, Bulliard V, Langendijk-Genevaux PS, Gasteiger E, Bairoch A, Hulo N: ScanProsite: detection of PROSITE signature matches and ProRule-associated functional and structural residues in proteins.
Nucleic Acids Res 2006, 34:W362-5. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Wiedemann N, Urzica E, Guiard B, Müller H, Lohaus C, Meyer H, Ryan M, Meisinger C, Mühlenhoff U, Lill R, Pfanner N: Essential role of Isd11 in mitchondrial iron-sulfur cluster synthesis on Isu scaffold proteins.
The EMBO Journal 2006, 25:184-195. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Adam AC, Bornhövd C, Prokisch H, Neupert W, Hell K: The Nfs1 interacting protein Isd11 has an essiential role in Fe/S cluster biogenesis in mitochondria.
The EMBO Journal 2006, 25:174-183. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Clarke CF, Williams W, Teruya JH: Ubiquinone Biosynthesis in Saccharomyces cerevisiae.
J Biol Chem 1991, 266(25):16636-16644. PubMed Abstract | Publisher Full Text
Steger HF, Söllner T, Kiebler M, Dietmeier KA, Pfaller R, Trülzsch KS, Propschug M, Neupert W, Pfanner N: Import of ADP/ATP Carrier into Mitochondria: Two Receptors Act in Parallel.
J Cell Biol 1990, 111(6):2353-2363. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Young JC, Hoogenraad NJ, Hartl FU: Molecular Chaperones Hsp90 and Hsp70 Deliver Preproteins to the Mitochondrial Import Receptor Tom 70.
Cell 2003, 112:41-50. PubMed Abstract | Publisher Full Text
Poon WW, Barkovich RJ, Hsu AY, Frankel A, Lee PT, Shepherd JN, Myles DC, Clarke CF: Yeast and Rat Coq3 and Eschericia coli UbiG Polypeptides Catalyze Both O-Methyltransferase Steps in Coenzyme Q Biosynthesis.
J Biol Chem 1999, 274(31):21665-21672. PubMed Abstract | Publisher Full Text
Jonassen T, Clarke CF: Isolation and Functional Expression of Human COQ3, a Gene Encoding a Methyltransferase Required for Ubiquinone Biosynthesis.
J Biol Chem 2000, 275(17):12381-12387. PubMed Abstract | Publisher Full Text
Grant B, Greenwald I: The Caenorhabditis elegans sel-1 gene, a negative regulator of lin-12 and glp-1, encodes a predicted extracellular protein.
Genetics 1996, 143(1):237-47. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Han C, Coe EH, Martienssen RA: Molecular cloning and characterization of iojap (ij), a pattern striping gene of maize.
The EMBO Journal 1992, 11(11):4037-4046. PubMed Abstract | PubMed Central Full Text
Purushothaman SK, Bujnicki JM, Grosjean H, Lapeyre B: Trm11p and Trm112p Are both Required for the Formation of 2-Methylguanosine at Position 10 in Yeast tRNA.
Mol Cell Biol 2005, 25(11):4359-4370. PubMed Abstract | Publisher Full Text | PubMed Central Full Text


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The 11U Georgia Shockers are looking for players to complete their spring roster. We will hold tryouts on September 3 and 4th, 2011. The Shockers are a AAA team playing all over Georgia. We practice and are based out of Lake Spivey Georgia. That's about 15 minutes south of the ATl airport at the border of Clayton and Henry County. We play about 12 tournaments per year not including state championships or the WS. We have had a successful 2011 season and plan to build on that success in 2012. 80% of our ballplayers have been with us for the past 3 years and are ready to add the final few that will complete our team. We are looking for experienced travel ball players including pitchers and catchers. We are big on developing ballplayers and having every kid prepared for the next level. If you think your kid is a proper fit for our program please give me a call and let me know. Thanks for your time.
Coach Patrick Joseph
I can't help with players but I may be able to help with tournaments. Look me up at eteamz.com/touchemallsports. All tourneys the first half of the season are there. You wont find many cheaper, I have games and High School Certified Umpires under the ISA Organization. The rules are the same as USSSA. I have 3 dates at Diamond Lakes. Thanks.
Matt Fleming
ISA Director
ACTIVE is the leader in online event registrations from 5k running races and marathons to softball leagues and local events. ACTIVE also makes it easy to learn and prepare for all the things you love to do with expert resources, training plans and fitness calculators.


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Policy modification data
I have one auditable query. I want the data of policy modifcation. When policy has change what change has been made and from which id.
I required the last 6 month data.
Any idea how to get it.
Comments 7 Comments • Jump to latest comment
Thanks In Advance
Ashish Sharma
you will get to know who made the changes however the changes that have been made is not currently available.
Help Link: http://www.symantec.com/business/support/overview.jsp?pid=54619
The Audit log contains information about policy modification activities, such as the event times and types, policy modifications, domains, sites, administrators, and descriptions.
The default Audit quick report is called Policies Used. View the Policies Used report to monitor the policies in use in your network, by group. You can look at the Audit log when you want to see which administrator changed a particular policy and when.
Check this - http://www.symantec.com/docs/HOWTO27244
Check these Articles:
About log types
About Computer Status reports and logs
About the different types of Symantec Endpoint Protection Manager Reports
For Client updates:
Monitors > Logs > [Log type] System > [Log content] Client Activity > Advanced Settings > [Event type] Installation events
If you're using AutoUpgrade method to upgrade your clients, you can also try the following:
Monitors > Logs > [Log type] System > [Log content] Client-Server Activity > Advanced Settings > [Event type] AutoUpgrade download
Reference: https://www-secure.symantec.com/connect/forums/sepm11-audit-logs
Hope that helps!!
Mithun Sanghavi
Associate Security Architect
MIM | MCSA | MCTS | STS | SSE | SSE+ | ITIL v3
Don't forget to mark your thread as 'SOLVED' with the answer that best helped you.
Have a look here:
About the information in the Audit report and log
...as per KB:
- audit report will give you information about policies being used and policy assignements
- audit log will show you all the policy related activities (policy added. edited etc.) - and what administrator has implemented the changes.


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New look: Coordinator Larry Coyer, pictured at practice Aug. 5, favors a more attacking defense than that of his predecessor, Ron Meeks.
- Filed Under
TERRE HAUTE, Ind. -- Reggie Wayne has noticed an edginess, and a difference, with the Indianapolis Colts defense.
"You can tell they're getting antsy," the three-time Pro Bowl wide receiver said Wednesday. "They want to tackle somebody."
They'll get their chance Friday night at Lucas Oil Stadium when the Colts open their preseason against the Minnesota Vikings. ...


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Retailers are starting to play hardball with the supply side of the produce industry. Some major chains are telling grower-shippers they will reject perfectly good -- even top-quality -- bulk produce unless it arrives already stickered with new standardized identification codes. The codes, called price look-up codes, or PLUs, are designed to add computer-based efficiencies to the produce department. While they have been in existence since 1990, it is only now, as companies search for new ...
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Moderators: Blitzen, Almighty Benny, Silent-sigfig
GeneralOfDeath wrote:(American) football, smart one.
Tzan wrote:Semaj Nagirrac wrote:Well, I took some land without checking if it was owned by a faction or not. I'm not going to be banned, am I? I can destroy everything if need be.
That's what Hitler said,
in 1938.
GeneralOfDeath wrote:Eh, every other nation on earth can't tell what a football is.
stubby wrote: my floppy penis gets first dibs on it for tradition's sake, but it doesn't seem likely that he'll want to stick around long enough to play.
Silverdream wrote:Baseball is shit. Play a real sport like hockey.
Ham701 wrote:GeneralOfDeath wrote:Eh, every other nation on earth can't tell what a football is.
Killer Karetsu wrote:Silverdream wrote:Baseball is shit. Play a real sport like hockey.
(baseball is okay too, as well as football...the normal and the american ones)
GeneralOfDeath wrote:Finland has always had crap gea, but good determination from their people. Canadia has alternatively had France, England and America covering its ass.
Users browsing this forum: Brikguy0410 and 3 guests


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I just lost myt collie a few months ago, looking to get a colden retriever puppy male or female, and how much will it cost me? aly told to call you. thank's
looking for a miniature golden retriever female
This site looks awesome!!! Keep up the good work!
Congratulations for all your beatiful dogs!! <3 Patrizia
Congratulations to Sasha and her new babies. We are so happy for you. I want another one Toni - Lola is ready for a sibling and she's doing great!! Karen :)
Tey r the cutest little things
Thank you for having us over to play with Underdog. He is sooooo cute and playful and are sure he will be an awesome member of some lucky family!
Hi,I'm a friend of Pam's. I recently lost my ten year old golden retriever. Pam emailed a picture of your puppy. Are there still two available? How much?
Hi Karen, Green collar and Mr. Horatio are available .... If you are interested and would like to see them Please call 732-693-8466... Thanks
What a great idea to memorialize the days you get to share with your "babies."They are gorgeous!I miss my Elvis & Mr Hoser so much & wish I had this for them!
Buddy- it is always a pleasure to see you. You are one of the best people I know and a true golden lover. Anyone who knowsmyoumis blesses!
Please keep me posted on Damiano Golden news. I am always interested to hear.
beautiful story - thanks for sharing!
we have a litter planned for end of summer. please forward your email address to email@example.com. We will forward a pup application to you or call 7326938466


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Members Picks
Page added on April 23, 2010
The Dallas Mavericks travel to San Antonio to play the Spurs in Game Three of the first round of the Western Conference Finals. Tip off at the AT&T Center in San Antonio is 9:30 ET. TV coverage is provided by ESPN.
With the series split 1-1, San Antonio will be looking to get ahead in their first home game of the series. The Spurs lost their first game in Dallas 100-94. San Antonio turned it around in Game Two last Wednesday beating the Mavericks 102-88.
Odds makers have the line set with the Spurs as 3.5 point favorites. The total is set at 193.5.
The Spurs are seeing great performances out of Tim Duncan. Duncan averaged 17.9 points, 10.1, rebounds, and 1.5 blocks per game during the regular season. In Game One in Dallas he had 27 points, two assists, and eight rebounds. In Game Two Duncan put up 25 points, two assists, and 17 rebounds. The veteran center will continue to be a key player and top performer in this first round series.
Dallas's seven foot Dirk Nowitzki has been a leader for the Mavericks all season, and his performance dictates how Dallas does in the rest of the series with the Spurs. Dirk had 36 points in the Mavericks' Game One win. He had seven rebounds and shot 12-12 from the free throw line. In Dallas's Game Two loss to San Antonio, Nowitzki shot 9-24 from the field and hit his one three pointer. He had 24 points, four assists, 10 rebounds, and hit 5-6 of his free throw attempts.
Spurs' guard Tony Parker has been a consistent force for San Antonio since his return from his injury. He has averaged 17 points, six assists, and 2.5 rebounds this post season while shooting 14-31 from the field.
Mavericks' point guard Jason Kidd did not have a good Game Two. After having 13 points, 11 assists, and eight rebounds in Game One, Kidd had just five points in 40 minutes of play in Game Two. Kidd hit one of four three-point attempts and both of his free throw attempts, going 1-7 from the field.
Though the Spurs took a game in Dallas, they have to face the best road team in the NBA. The Mavericks were 28-13 at home and 27-14 on the road this season. San Antonio has not beaten the Mavericks in a playoff series since 2003.
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thepastorsplace Video Channel
- Channel Views: 4,419
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- Subscribers: 10
- Joined: October 01, 2010
- Last Visit Date: 2010-10-28T14:33:42
I am a licensed minister and a licensed Clinical Christian/Pastoral. I hold a Master's Degree in Clinical Christian/Pastoral Counseling and am working on my Doctorate Degree. I am married, have 2 grown daughters and 4 grandchildren.
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A woman chatting on her cellphone while walking to her car has the phone grabbed out of her hand by a robber at Quail Springs Mall.
A knife-wielding robber confronts a man in the Crossroads Mall parking lot. The driver jumps out of his vehicle, leaving his keys in the ignition. The thief takes the car.


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Professional Fast Economical Snowmobile shock service
I have nine years of shock rebuilding experience, 20 years as a mechanical engineer with 6 years of hydraulic component and system design experience and I am a Fox dealer.
I service Fox, ACT, RydeFX, HPG and Walker Evans brands using Amsoil Shock Therapy synthetic shock oil. Rebuildable shocks should be serviced once a year or every 2000 miles. New shocks need to be rebuilt after 300- 500 miles to remove the contaminates like a new 4 cycle engine needs an initial oil change.
Standard shock rebuild rates are only $25 each for all brands that I service. Polaris position sensitive rear track shocks are $35 each. Fox, ACT and RydeFX clickers or reservoir shocks are $35 each. FOX Floats are $35 each. All Walker Evans and HPG Clickers are $45 each. The Arctic Cat dial adjust front track shocks are $55 each. I have the update for the seperator piston in the ACT Dial Adjust for and additional $30. I also do Sno-Pro cross link shocks for $120. These prices include oil only, parts are extra if required. Normally I don't replace seals unless they are leaking. Seal kits are $12.50 per shock for FOX and $14.00 for ACT shocks. I only use Tech-Syn seals. I have the update for the front track shock on the '06 XF std to Sno Pro specs when you change the front track spring to the SP. $75 includes a new valve stack, piston and rebuild. I can also repair most ACT and Fox shocks if the rods are bent or broken and even the bodies or end caps can be replaced. This is always less than a new shock would cost. Turn around is normally 2-3 days from the day I get the shocks and usually only a day or two more if I don't have the parts in stock. I'm located in Plymouth MN near the intersection of 394 & 494. I accept cash, checks or Paypal for those who need to ship their shocks.
If you need to ship your shocks remove all the springs if you can get them off as this saves on shipping costs. Losen the adjuster nut and compress the spring by hand and remove the retainer. If you think you need shafts or seals the best way to go is Paypal as I can get you the exact costs before you pay and after I inspect the shocks. The best way to ship is UPS or FedEx ground. FedEx is about 30% less than UPS. UPS has lots of stores since buying Mail Boxes etc. FedEx bought Kinkos so you can ship from there too. Go to UPS.com or Fedex.com and search for the nearest store to you. You can pay buy using Paypal, money order or personal check. For a personal check I will hold you shocks for seven days from the day I receive the shocks to insure the check clears. Paypal is firstname.lastname@example.com. Put them in a box with lots of packing material as the get thrown around and include a check or money order covering rebuilding and return shipping ($10 for the 5 state area or $15 for all other states) or you can use Paypal. Also include your contact information and return address. Include both phone number and email as well as the exact make, model and year of the snowmobile the shocks came from. Call or e-mail Dave with any questions.


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- Home
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HAT off-piste club
This is all designed to help you answer the question ... is it safe out there?
As we teach, the answer to this is – it all depends! At HAT we want to help you understand what it depends on, so you can make better informed decisions and have more fun.
The HAT off-piste club is a community of free riders and off piste skiers who share similar values and beliefs about free riding. We want to be well informed, because we know that means we will have more fun AND we will stay safe.
The HAT off-piste club provides in-depth self help training materials, opportunities to get personal advice from Henry, live Avalanche Talks and on snow training programmes.
Click to register for free
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We also offer Premium membership.
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Premium members can
- Study all of the HAT training materials online. This includes
- Ride Hard Ride Safe – an introduction to off piste safety & avalanche awareness in 4 parts. This totals more than two hours of video and presentation materials that help you answer the question "is it safe out there?"
- In depth and advanced modules covering key topics in further depth
- How and why avalanches trigger - an indepth module helping you understand why avalanches trigger and to identify the implications for your decision making
- Where you go and when - an in depth look at how to figure out where the best snow is and still stay safe
- How you go down or up - an in depth look at route selection and how to make sure you and your group are safe on the slope whilst still enjoying the best snow.
- Being well prepared - A number of resources covering subjects like having the right equipment, beeper training theory, creating a positive group psychology, managing the human factor
- Access to premium HAT webcontent offering insights on the evolving conditions, the snow conditions, the weather, the weak layer, the bulletins.
And as an extra bonus, premium members can
Get 10% off everything we sell on the HAT website, this includes talks, on snow programmes, equipment, off-piste guiding. This alone will cover your membership fee.


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Sixty Hurricane Sandy Pets Fly First Class To New Holiday Home
A general view of atmosphere of sixty orphaned dogs and cats who are... News PhotoAbundance,Airport,Animal,Arts Culture and Entertainment,Atmosphere,Dependency,Dog,Domestic Cat,General View,Horizontal,Hurricane Sandy,New Jersey,Newark - New Jersey,Newark International Airport,Orphan,USAPhotographer Neilson BarnardCollection: Getty Images Entertainment 2012 Getty ImagesNEWARK, NJ - NOVEMBER 17: A general view of atmosphere of sixty orphaned dogs and cats who are being flown across the country from Newark Liberty International Airport on November 17, 2012 in Newark, New Jersey, to make room for thousands of animals who need shelter as a result of Hurricane Sandy. SeaWorld's animal rescue team assists in the transport, which takes place aboard a donated Southwest Airlines flight. The pets are flying to the Helen Woodward Animal Center in San Diego, California, where they will be placed in loving homes with adoptive families. (Photo by Neilson Barnard/Getty Images for SeaWorld)


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2 - Narnia and its crtitics
Taken from The Lion's World
File informationSize: 82.5KB
Format: PDF Document
Narnia and its critics
I mentioned earlier the way in which Philip Pullman had conceived his own trilogy as a sort of riposte to the Narnia books. The parallels are clear enough – an alternative universe including talking animals, even (though Pullman might dispute this) an active and independent heroine, if we give due weight to how Lucy is portrayed in The Lion. And it is possible to see how Pullman's aims correspond to Lewis's as we have just explained them. Pullman's parallel world is one in which we encounter God in a way that strips away the conventions that in our world conceal his real nature – only, in Pullman, that 'real nature' is a fiction. It is something projected by those who want arbitrary power, a great fantasy that sanctions systematic abuse, oppression and corruption. The God of these stories is 'objectively' no more than a self-deluded angel, now senile and helpless, but allowed to survive because he is the symbolic focus and rationale for unaccountable human authority...
Available to download by subscribers using 1 credit
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- Notes
- Preface The Lion's World
- Notes
- Lenten reading guide
- Questions for reflection or group study
- About the author
- Conclusion
- 5 - The silent gaze of truth The Lion's World
- 4 - No story but your own The Lion's World
- Introduction -1


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A robotic vocal sound made using Propellerhead's Reason Maelstrom syn. Programmed by myself and further processed in Wavelab. It sounds a bit like the word's "No I don't.
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I recently played my first playoff game in Fiji, against Navoci village. The other team thought it was amusing to target every kickoff at me, and then send their largest player barrelling at me full speed as a way to "kaivulagi" (foreigner). On one occasion, I caught the kick with my arms up but without jumping, leaving myself in a vulnerable position. I took one of the hardest hits I've ever felt right in the low end of my back, as I turned to dump the pass to our scrum half. I hopped up quickly and did my best to hobble as fast as I could so the other team wouldn't have the pleasure of thinking they had hurt me.
I continued to play, albeit slowed with significant tailbone pain. I hung in there until someone punched me in the ruck. I continued to hit the next ruck while I noticed something was pouring out of my head. I was forced to leave the game by the ref to get stitched up, but as I left I made sure to let the other team know that they hadn't gotten rid of me, and that I was going to return soon.
I got my head fixed up at just about half time. I shouted a taunt to the opposing sideline and went to join my team for the break. Normally, to my disappointment, I get pulled at the second half, and this was one time I was looking forward to it- tailbone woes, a bloody head and a bit dizzy from how tightly the nurse had taped my head. The coach asked how I felt and for some foolish reason I said "great", at which point I was informed that I was headed back in for the second half.