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|World J Gastroenterol 2002 August 15;8(4):650-653
|Email: firstname.lastname@example.com
|http:// www.wjgnet.com
|Copyright © 2002 by The WJG Press
Metallothionein expression in hepatocellular carcinoma
Geng-Wen Huang, Lian-Yue Yang
Geng-Wen Huang, Lian-Yue Yang,
Department of General Surgery, Xiangya Hospital, Central South University,
Changsha 410008, Hunan Province, China
Supported by the Science Fund of Department of Science and Technology of Hunan Province, No. 98ssy1008
Correspondence to: Dr. Geng-Wen Huang, Department of General Surgery, Xiangya Hospital, Central South University, Changsha 410008, Hunan Province, China. firstname.lastname@example.com
Telephone: +86-731-4350637
Received 2001-12-05 Accepted 2002-02-07
AIM: To investigate the expression of metallothioneins (MTs), which were recently thought to have close relationship with tumors, in human hepatocellular carcinoma.
METHODS: Histological specimens of 35 cases of primary human hepatocellular carcinoma with para-neoplastic liver tissue and 5 cases of normal liver were stained for MTs with monoclonal mouse anti-MTs serum (E9) by the immunohistochemical ABC technique.
RESULTS: MTs were stained in the 35 cases of HCC, including 6 cases negative (17.1 %), 23 weakly positive (65.7 %), and 6 strongly positive(17.1 %). But MTs were stained strongly positive in all the five cases of normal liver and 35 cases of para-neoplastic liver tissue. The differences of MTs expression between HCC and normal liver tissue or para-neoplastic liver tissue were highly significant (P<0.01). The rate of MTs expression in HCC grade I was 100 percent, higher than that in grade II(81 %) and grade III and IV (78 %). But the differences were not significant (P>0.05). No obvious correlations between MTs expression in HCC and tumor size, clinical stage or serum alpha fetoprotein concentration were found (P>0.05).
CONCLUSION: Decrease of MTs expression in HCC may play a role in carcinogenesis of HCC. MTs are stained heterogenously in HCC. We can choose the anticancer agents according to the MTs concentration in HCC, which may improve the results of chemotherapy for HCC.
Huang GW, Yang LY. Metallothionein expression in hepatocellular carcinoma. World J Gastroenterol 2002; 8(4):650-653
Metallothioneins (MTs) are a family of low-molecular weight, cysteine rich proteins which are widely distributed in various species. MTs are thought to be involved in heavy-metal detoxification, intracellular trace elements storage and scavenging free radicals. Recently, emerging data suggest that MTs have close relationship with tumors. They might play important roles in carcinogenic and apoptotic process and differentiation of tumor cells[1-12]. And besides, MTs are attributed to affording tumor cells resistance to some important chemotherapeutic agents[13]. This study is aimed to examine MTs expression in human hepatocellular carcinoma (HCC) and to explore its biological and clinical significance.
Clinical material
The clinical information and pathological specimens from 35 patients, whose liver tumors were removed at XiangYa Hospital from 1998 to 2000, were analyzed. All the cases were reviewed to confirm the pathological diagnosis of HCC. All the 35 specimens of HCC contained their corresponding para-neoplastic liver tissue. The ages of patients ranged from 31 to 71 years, with a mean��SD of 49.92��9.32 years. 33 were men and 2 women. According to Edmonson pathologic grading, there were 5 cases of grade I, 16 grade II and 14 grade III-IV. According to the clinical staging of UICC, there were 22 cases of stage II, 5 stage III and 8 stage IV. All the cases had positive HBsAg. This study also included 5 cases of normal liver tissue, around the hepatic hemangioma excised also in XiangYa Hospital.
Immunohistochemical determination of MTs
All the specimens were fixed in 10 % buffered formalin and embedded in paraffin. Five-micrometre thick sections were cut from the tissue blocks, mounted onto glass slides and were used for staining. ABC technique was adopted. Briefly, 5 mm sections were deparaffinized and rehydrated first, and then were immersed in 3 % H2O2 with methanol for 30 minutes to remove the endogenous peroxidase activity. Sections were further incubated with 10 % normal goat serum for 1 hour, followed by incubation with monoclonal mouse anti-MTs serum (E9) (1:50) at 4 �� overnight. The sections were then washed in phosphate buffer solution (PBS) (0.01M, pH 7.2) and they were sequentially incubated with: (1) biotinylated goat anti-mouse IgG, and (2) avidin-biotin horseradish peroxidase complex following the manufacturers` instruction (ABC kit, BOSTER Ltd. Wuhan). Staining was developed by immersing slides in 0.05 % 3,3?diaminobenzidine tetrahydrochloride (DAB) with 0.33 % hydrogen peroxide. All slides were counterstained with haematoxylin, dehydrated and mounted. PBS substituted for the primary antibody was used as the negative control. According to the proportion of positively stained cells, a grade was given from I to III, with Grade I indicating less than one third of cells stained and Grade III indicating more than two thirds of cells stained. The intensity of MTs expression was also graded and given a grade for 0 to II, with Grade 0 indicating no staining, and Grade II indicating the greatest intensity of staining. A weighted score was then generated to semiquantify the MTs expression level in the tissue by multiplying the MTs intensity score with the proportion of the positively stained tumors cells. A weighted score of zero indicated no MTs staining (-), more than 3 indicated strongly positive MTs staining (++) and that between zero and 3 indicated weakly positive MTs staining (+)[14].
Statistical analysis
The data were expressed semiquantitively. Ridit test was used to determine the difference. The results were considered statistically significant when P<0.05.
All negative control slides showed no staining for MTs, demonstrating the specificity of the monoclonal antibody E9. Strongly positive MTs immunoreactivity was observed in all the five control normal liver sections, mostly in cytoplasm with a few in nucleus. All the surrounding connective tissues, including blood vessels and bile ducts were negative for MTs staining. Twenty-nine of 35 HCC showed positive MTs staining, including 6 strongly positive (17.1 %) (Figure 1), 23 weakly positive (65.7 %) (Figure 2) and 6 negative (17.1 %) (Figure 3) . MTs expressed strongly in all the para-neoplastic liver tissue. The differences of MTs expression between HCC and para-neoplastic liver tissue or normal liver tissue were highly significant (Table 1, P<0.01).
Figure 1 Strongly positive staining of MTs in hepatocellular carcinoma ABC ��200
Figure 2 Weakly positve staining of MTs in hepatocellular carcinoma ABC ��200
Figure 3 Negative staining of MTs in hepatocellular carcinoma ABC ��200
The positive ratio of MTs expression in grade I of HCC was 100 %, higher than that in grade II (81 %) and grade III and IV(78 %). But the differences did not reached the level of significance (P>0.05). In addition, the differences of MTs expression in HCC with different clinical stages, tumor sizes and serum alpha fetoprotein concentration were not significant either (Table 2).
Table 1 Levels of MTs expression in HCC, para-neoplastic and normal liver tissues
|MTs score
|Para-neoplastic tissueb
|Normal liver tissueb
bP<0.01, vs HCC
Table 2 Correlation between MTs expression and clinicopathologic data of HCC
|MTs score
|Pathologic grade
Grade I
|Grade II
|Grade III-IV
|Clinical stage
|Stage I-II
|Stage III-IV
|Size (mm)
|AFP concentration (ng/ml)
Recently, much attention was paid to the association between MTs and tumors. Available information suggested that MTs might play important roles in carcinogenic and apoptotic process of some tumors[1-13,15-20]. Using immunohistochemical staining method, MTs have been localized intensively in various types of human tumors in organs and tissues such as skin, kidneys, prostate, testes, gallbladder, colon, breast and endometrium[15-32]. It was mainly attributed to loss of control of the transcription of MTs genes, which was caused by activation of some oncogenes, such as Ha-ras, in tumor cells[33]. However, the potential roles of MTs in carcinogenic process was not yet well understood[34]. Several pieces of evidence suggested that MTs could combine with and sequester those carcinogenic substances in cells. When the cells were invaded by the carcinogenic substances, many protective processes were activated to scavenge those substances. Elevation of MTs may be one of the processes[35-40]. But not all the tumors contained elevated MTs[41], such as HCC. In this study, the authors have detected MTs expression in 35 cases of HCC and their corresponding para-neoplastic liver tissue and 5 cases of normal liver tissue. The results showed that MTs were strongly positive in all the normal liver tissue and para-neoplastic liver tissue, considerabely higher than those in HCC tissue, which conformed to the finding of Deng[14]. MTs are functional proteins in liver tissue, which is involved in heavy metal detoxification. The down-regulation of MTs expression in HCC suggested that these cells probably have different proliferative or differentiated characteristics as compared to the surrounding normal hepatocytes, and MTs may be a marker of hepatocellular differentiation. In addition, this down-regulation might play a role in carcinogenic process of HCC. MTs are one of the most important intracellular free radicals scavengers, and oxidateve injury may be one of the most important causes of gene mutation. As a result, this down-regulation of MTs expression might cause accretion of oxidative in cells, which might lead to activating some oncogenes or inactivating some tumor-suppressor genes[38-40].
Several other studies have also shown that the MTs levels may be related to the degree of differentiation of tumor cells. For example, using immunohistochemical staining method, a distinct difference between seminoma and nonseminoma of human testicular tumors was found[21]. Well-differentiated seminoma showed litter or no staining for MTs while less differentiated nonseminoma (embryonic carcinoma) strongly expressed MTs. MTs overexpression in human breast carcinoma, colorectal carcinoma, esophageal carcinoma, osteosarcoma, endometrial carcinoma and melanoma tended to correlate with poor prognosis[19,24-42]. In this study, the positive rate of MTs expression in grade I of HCC is 100 percent, higher than grade II (81 %) and grade III and IV (78%), but the differences have not reach the level of significance. So the genuine relationship between MTs and differentiation of HCC remains to be further investigated.
Resistance to antineoplastic agents is one of the major obstacles to curative therapy of HCC. The development of this resistance has been explained by several factors, including increased DNA repair processes and expression of MDR gene[43]. In 1998, Kelley et al[44] found that tumor cell lines with acquired resistance to the antineoplastic agent cisplatinum overexpressed MTs and demonstrated cross-resistance to other eletrophilic anticancer agents, such as melphalan, chlorambucil. Furthermore, cells transfected with bovine papilloma virus expression vectors containing DNA encoding human MTs were resistant to electrophilic anticancer drugs mentioned above, but not to 5-fluorouracil or vincristine. Thus, overexpression of MTs represented one mechanism of resistance to a subset of clinically important anticancer drugs. Those results were substantiated by a series of other experiments[45-49]. The mechanism that MTs were involved in drug resistance might be that MTs in tumor cells could combine with those electrophilic anticancer drugs and prevent them from acting on their targets, such as DNA. In this study, we have shown that MTs expressed heterogenously in HCC, including 6 cases with strong expression, 23 with weak expression and 6 negative. The authors therefore assumed that resistance to electrophilic antineoplastic agents in a portion of HCC might be related to MTs expression in tumor tissue.
Thus, anticancer agents could be chosen according to the levels of MTs expression in order to improve the efficiency of chemotherapy for HCC. For example, electrophilic antineoplastic agents were used in the treatment of HCC without MTs and non-electrophilic antineoplastic agents were used in HCC containing high levels of MTs. However, the effect of this design remains to be determined. Recently, a gene therapy by transactivating MTs to the chemoresistant tumour cells to reverse the chemoresistance have been reported[50]. In the near future, oncologists are really able to use the knowledge about MTs in overcoming cancer.
The down-regulation of MTs expression in HCC might play roles in carcinogenesis of HCC, but its biological and clinical significance is still uncertain. A further understanding of the association between MTs expression and resistance to anticancer agents should facilitate the chemotherapy for HCC.
1 Takaba K, Saeki K, Suzuki K, Wanibuchi H, Fukushima S. Significant overexpression of metallothionein and cyclin D1 and
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11 Jin R, Chow VT, Tan PH, Dheen ST, Duan W, Bay BH. Metallothionein 2A expression is associated with cell proliferation in
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17 Naito S, Koga H, Yokomizo A, Sakamoto N, Kotoh S, Nakashima M, Kiue A, Kuwano M. Molecular analysis of mechanisms
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18 Janssen AM, van Duijn W, Oostendorp-Van De Ruit MM, Kruidenier L, Bosman CB, Griffioen G, Lamers CB, van Krieken JH,
van De Velde CJ, Verspaget HW. Metallothionein in human gastrointestinal cancer. J Pathol 2000; 192: 293-300
19 Jin R, Bay BH, Chow VT, Tan PH, Lin VC. Metallothionein 1E mRNA is highly expressed in oestrogen receptor-negative human
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20 Ioachim EE, Kitsiou E, Carassavoglou C, Stefanaki S, Agnantis NJ. Immunohistochemical localization of metallothionein in
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23 Shukla VK, Aryya NC, Pitale A, Pandey M, Dixit VK, Reddy CD, Gautam A. Metallothionein expression in carcinoma of the
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24 Zelger B, Hittmair A, Schir M, Ofner C, Ofner D, Fritsch PO, Bocker W, Jasani B, Schmid KW. Immunohistochemically
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26 Douglas-Jones AG, Schmid KW, Bier B, Horgan K, Lyons K, Dallimore ND, Moneypenny IJ, Jasani B. Metallothionein expression
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28 Sens MA, Somji S, Garrett SH, Beall CL, Sens DA. Metallothionein isoform 3 overexpression is associated with breast cancers
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29 Jasani B, Schmid KW. Significance of metallothionein overexpression in human tumours. Histopathology 1997; 31: 211-214
30 Rossen K, Haerslev T, Hou-Jensen K, Jacobsen GK. Metallothionein expression in basaloid proliferations overlying
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31 Zhang XH, Jin L, Sakamoto H, Takenaka I. Immunohistochemical localization of metallothionein in human prostate cancer.
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32 Giuffre G, Barresi G, Sturniolo GC, Sarnelli R, D�Inca R, Tuccari G. Immunohistochemical expression of metallothionein in
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response. Cancer Res 1998; 58: 2335-2338
35 Ioachim EE, Goussia AC, Agnantis NJ, Machera M, Tsianos EV, Kappas AM. Prognostic evaluation of metallothionein
expression in human colorectal neoplasms. J Clin Pathol 1999; 52: 876-879
36 Sutoh I, Kohno H, Nakashima Y, Hishikawa Y, Tabara H, Tachibana M, Kubota H, Nagasue N. Concurrent expressions of
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38 McCluggage WG, Maxwell P, Hamilton PW, Jasani B. High metallothionein expression is associated with features predictive
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39 Rossman TG, Goncharova EI. Spontaneous mutagenesis in mammalian cells is caused mainly by oxidative events and can be
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41 Duncan EL, Reddel RR. Downregulation of metallothionein-IIA expression occurs at immortalization.
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42 Florianczyk B, Grzybowska L. Metallothione in levels in cell fractions from breast cancer tissues. Acta Oncol 2000; 39: 141-143
43 Xu BH, Zhang RJ, Lu DD, Chen XD, Wang NJ. Expression of mdr1 gene coded pglycoprotein in hepatocellular carcinoma and its
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44 Kelley SL, Basu A, Teicher BA, Hacker MP, Hamer DH, Lazo JS. Overexpression of metallothionein confers resistance of
anticancer drugs. Science 1988;241:1813-1815
45 Kondo Y, Kuo SM, Watkins SC, Lazo JS. Metallothionein localization and cisplatin resistance in human hormone-independent
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46 Miyazaki H, Naitoh Y, Nakahashi Y, Yanagitani S, Kuno K, Ueno Y, Okajima A, Inoue K. Induction of metallothionein isoforms
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47 Shnyder SD, Hayes AJ,Pringle J, Archer CW. P-glycoprotein and metallothionein expression and resistance to chemotherapy
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48 Moriyama-Gonda N, Igawa M, Shiina H, Wada Y. Heat-induced membrane damage combined with adriamycin on prostate
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49 Kikuchi Y, Hirata J, Yamamoto K, Ishii K, Kita T, Kudoh K, Tode T, Nagata I, Taniguchi K, Kuwano M. Altered expression of
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Edited by Zhang JZ


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Bushfires Burn Across Victoria
A tree trunk smolders after a fire on January 19, 2013 in Melbourne,... NachrichtenfotoAustralien,Baum,Brennen,Feuer,Melbourne,Unfall und Katastrophe,VertikalPhotographer Craig SillitoeCollection: Getty Images News 2013 Getty ImagesMELBOURNE, AUSTRALIA - JANUARY 19: A tree trunk smolders after a fire on January 19, 2013 in Melbourne, Australia. Bushfires in Victoria have claimed one life and destroyed several houses. Record heat continues to create extreme fire conditions throughout Australia. (Photo by Craig Sillitoe/Getty Images)


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Hi all,
We have recently set up a facebook group called FACEPLANT so that we can organise day trips, weekend epics and pissups easier.
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'We want them to succeed' - Free language classes a fun challenge
Eulalio "Lalo" Contreras said he understands English but struggles to speak it.
For the second time in 15 years, Contreras is working toward being fluent in English. With about 10 other students, he has registered and committed to attend English classes every Wednesday from February to May at Southland Baptist Church.
The free classes have ongoing registration, said Karen Sanders, an instructor at Southland.
Contreras, who has been in the United States for more than 22 years, said his prior attempt to learn the language didn't get him far.
"It wasn't the same" as this program, he said. "Here, they explain what a word means."
Before, Contreras recalled, he would answer to satisfy the teacher — even when he didn't understand.
This time around, his goals are to improve his writing skills, he said, and to enunciate words correctly.
"It is always a struggle because you need it at work all the time," Contreras said.
Contreras, a busboy at Franco's Restaurant, is one of four Franco's employees taking the classes.
Sanders, certified to teach English as a second language, said the classes are designed to teach English to anyone regardless of his or her native language, but all students registered in March are Spanish speakers.
The classes are divided into three levels — beginning, intermediate and advanced — that a student places into based on a verbal test with Sanders.
Veterinarian Guy Sheppard volunteers as an instructor.
"I've always liked teaching, especially when you have a willing student," Sheppard said. "I just can't imagine the obstacles (they face) — it impresses me beyond expectations."
All instructors underwent training through the Adult Literacy Council, and the center provided learning materials for the program's use.
Sheppard and Sanders said they speak a little Spanish, but it's not necessary for teaching English to Spanish speakers.
"You have to immerse yourself" as a student, Sanders said. "If you start translating (for them), it's not as quick."
The instructors must understand special considerations when teaching English to Spanish speakers, Sanders said.
"A," "e," and "i" are difficult for Spanish speakers learning English, she said. The English sound for "e" is the same as "i" in Spanish, and "e" in Spanish might sound like "a" in English.
Teaching English, like any other subject, also requires ingenuity in methodology.
"You can't make it boring," Sanders said. "You have to break it up."
While teaching a small group last March, Shepherd mixed sentence structure exercises, word rules, rhyming games and conversation to engage the students.
"We don't want them to be overwhelmed," said Inger McManus, also an instructor. "We want them to succeed."
On the other hand, McManus said, challenging them is necessary to ensure that their time is well-invested.
The English classes are expected to resume in the fall, Sanders said. In addition, Sanders said, she hopes to offer citizenship classes sometime in the future.
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To learn more
- What: Free English classes.
- Where: Southland Baptist Church, 4300 Meadow Creek Trail.
- When: From 6 p.m. to 8 p.m. Wednesdays.
- What else: Contact Karen Sanders, instructor, at (325) 949-9633. Childcare is available.
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The Florida Grand Opera closes out its season next week with Massenet's "Thais," and tickets to select performances are up to 40% off for the opera about the captivating courtesan and the monk who believes he needs to save her soul.
The following seating sections are available at the Arsht Center in Miami on Saturday, May 3, at 7 p.m. and Sunday, May 4, at 2 p.m.:
Note: Lower priced tickets are available for the Sunday matinee performance.
Or catch performances at the Broward Center in Fort Lauderdale on Thursday, May 15, at 7:30 p.m. and Saturday, May 17, at 7:30 p.m.:
The opera takes place in ancient Egypt, and includes one of the most well-known pieces in classical music, the intermezzo for solo violin and orchestra in Act II, known as "The Meditation."
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Disneytoon Studios new adventure The Pirate Fairy features Zarina, a smart and ambitious dust-keeper fairy whose wild ideas get her into trouble.
And according to filmmakers, a fairy with such an outgoing personality called for a very special musical accompaniment. ³We are lucky enough to
have award-winning Natasha Bedingfield serve as the inner voice of Zarina through her songs, says Peggy Holmes, director of The Pirate Fairy. Zarina is a little offbeat and contemporary and these songs showcase her personality so well. We used one of Natashas songs, Weightless, as a temp track and fell in love with itso much so, we decided to use it in the final film. We felt Natashas voice so perfectly spoke to Zarina character, we asked her to also perform our original song Who I Am, which
was written for The Pirate Fairy. Says Bedingfield,Being part of The Pirate Fairy was fantastic, I like pirates and I really love fairies, so I could think of nothing better than working on a song for the movie that brings these things together. It really has everything I love in it.
"Disclosure: This post may contain affiliate links. By clicking these links you are supporting the Saving4five Blog. For product reviews I am sometimes given free product in exchange for my honest opinions. Regardless, I only recommend products or services I use personally and believe will be good for my readers. The review are the honest, unbiased opinion of the writer only."


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I've been told by a dear friend that I don't blog enough. It's true, and I have nothing to say for myself. When the spirit moves me, I blog. And besides, there is a lot of behind the scenes stuff keeping me busy. All in due time.
BUT! There is a very exciting event happening in my part of the world, and it is quickly approaching, and if you live around here — the Piedmont Triad area of NC, Central NC, etc. — then this announcement is for you!
As you can see, I am helping (that term being used v. loosely) my friend Cindy host a Knit-In & Yarn Swap! I've been to a fiber swap before, but I happen to be a yarn swap virgin. So I am super-duper excited. I need to go through my stash before next Sunday to see what I'm willing to trade, and I'll probably bring copies of my patterns to sell, too.
If you plan on coming, please drop Cindy a line…her email is listed above. Bring your unloved stash, and bring friends! See you there!
I can't do much, but I'm doing a small bit. For all End of May hat or mittens patterns sold in my Pattern Store or on Ravelry between January 15th and January 31st, I will donate $1 to Doctors Without Borders to help with relief efforts in Haiti and worldwide. If you've been thinking of buying either or both patterns, now is the time!
Many designers are donating a portion of their pattern sales, and I urge you to support their efforts! To browse all the "Help for Haiti" patterns on Ravelry, see this page. You can even refine your search to include "Help for Haiti" patterns that you have queued, or that you have favorited.
Thank you!


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|Brietenstein will start for Terriers
|Written by Mike Kelly
|Tuesday, 31 August 2010 18:19
Wofford College coach Mike Ayers has announced that Eric Brietenstein will get the starting nod at fullback on Saturday when the Terriers open the 2010 season at Ohio University.
Breitenstein missed most of last season with a knee injury. He was injuried during the second game of the season against Charleston Southern. Brietenstein, who was named to the Southern Conference All-Freshman Team in 2008, ran for 187 yards on 28 carries and three touchdowns before the injury.
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Developed by InteraXon, a Canadian based company, the Muse is a brand new brain wave reading neuro-headset and a sexy one at that. Currently in the concept phase the Muse has recently launched on the crowd funding website Indigo and is set for public release spring 2013, if you fancy your name in the credits of the first application you can contribute here.
Since 2007 InterXon and has been a leading figure in thought-controlled computing, with Areil Garton the CEO becoming synonymous with the brain-computer interface market. InterXon's most publicised event to date being the illumination of landmarks at the 2010 Vancouver winter olympics, where visitors could don a neuro-headset and control a light show by focusing or relaxing their minds.
Like existing neuroheadsets such as Emotiv's Epoc or NeuroSky's MindWave and MindWave Mobile, the Muse has sensors in the headband positioned on the forehead and ears that monitor brain activity. The Muse is like a heart rate monitor for the brain, electrical signals are collected in real-time from the brain using four medical grade electroencephalography (EEG) sensors imbedded within the flexible headband, once synced with a downloadable app just like you would a mobile phone, the user can track and improve their mental health and agility through the monitoring of mood and focus levels.
The benefits from this type of brain training are far-reaching because the brain is the control system for the whole body. It's not just the cognitive benefits but also the physiological and psychological benefits that come with neurofeedback training. At the outset I was told that neurofeedback training as an alternative therapy was only for people with ADD, ADHD and associated mental and stress disorders, I quickly realised this is simply not the case and training your state of mind through the direct measurement of your brainwaves can be beneficial for any human being in any given situation.
The alternative therapy market and technologies for wellness and lifestyle monitoring are developing rapidly, we've already seen products such as Nike's Fuel band amongst others and of course there are now several B-CI manufacturers. In the UK interest in these areas from the national press has really started to gain momentum in the last 6 months and the next 6 months should be really interesting.
Read the full indigo article.
If you haven't already check out the InterXon Blog.


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The Boulder Outlook Hotel has won the Blues Foundation's 2013 Keeping the Blues Alive award for a club — the only recipient in a nationwide contest. It's bittersweet news, since by 2014, the hotel will most likely be demolished and converted into student housing.
"We are under contract to sell to a private group," says hotel owner Dan King, noting that the deal is not yet final. "Their plan is not to put a shovel in the ground probably before 2014."
King expects the deal with Austin-based American Campus Communities (ACC), a publicly traded company with more than $4 billion of outstanding shares, to close by the end of the year. The Outlook property would mark an expansion of ACC's holdings in Boulder; the company already operates Bear Creek Apartments and University Village at Boulder Creek.
In the meantime, King expects to operate the hotel and its blues venue on an interim basis until 2014.
"We know we'll be here for at least another year," he says. When asked why the time was right to sell, King said, "Because people started offering us enough money. That's frankly it. The price started going up."
The Blues Foundation offers the Keeping the Blues Alive award to one club across the nation each year. In a press release, the foundation said, "The Boulder Outlook Hotel is an intimate, 120-seat room, which has presented the finest blues talent to its customers. ... In recent years, the roster of talent that has played at the Outlook is a who's who in the blues."
King and a number of investors purchased the hotel in December 2002. In 2005, the hotel began hosting live music. Now, there's music five times a week or more at the hotel, with an emphasis on the blues.
King doesn't know whether he will look for another venue to operate. It was always a effort of passion, not business, for him, he says.
"I don't make much money doing music here," he says. "It really is about the love of the music and supporting the community."
But the music side benefitted from the hotel's business, King says. Musicians traveling from coast to coast could stay at the hotel and perform a show or two right next door. By offering a place for major acts to stop in for a few days, the hotel was able to attract major names, such as Tommy Castro and Janiva Magness, that rarely play in 120-seat venues.
"We've been very lucky and fortunate in that because we have the hotel, we've been able to get some headlining people that normally wouldn't play a room our size, but we'll pick them up on a Tuesday or a Wednesday night when they're traveling out to California or coming back from California," King says. "It gives them a break and they get to play a small room."
As for the future of blues in Boulder, King is upbeat. The Colorado Blues Society also won a Keeping the Blues Alive award, for a blues society, and King credits a healthy local scene in part to the efforts of the society.
"The blues scene, for what it is, has really gotten strong in the last few years. It's really changed since I started doing it, and since these guys have gotten more active, they have really good leadership out there," King says. "The number of guys who are out there who are local doing music is just phenomenal."
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Web site is expected to achieve overall profitability within a year (Reporter Xue Song) recently, the public comment public comment next year CEO Zhang Tao denied the rumors at the time of the listing interview with this reporter, said there was no listing in the short term. 'We are not short of money, a large number of financing is coming in last year, so we do not have the capital side of the pressure, comments on the listing Air Jordan Aero Flight or not is not very valued.' Tao said that more than 100 million US dollars is eligible for financing reported, this platform for local consumption to get more than 100 million US dollars last year, bottom round of financing. This four venture capital firms Nike Shox Turbo 13 are: Zhi letter Capital, Sequoia Capital, Qiming Venture Partners, Lightspeed Venture Partners. The financing is the third financing public comment. January 2006, the first round of financing public comment, as investors Sequoia Capital. May 2007, a second round of public comment network of financing, the investor for Google public comment beginning to achieve profitability in 2008, after which the annual income are more than 200% growth 'in 2010 we had wanted to market, then if $ 1 billion valuation is able to do, I will feel very happy, because then the public comment is one of China's Yelp (US review site). 'CEO Zhang Tao said public comments. As we all know, because of the rise of buy mode, then Nike Shox Roadster 12 seven years of hard work has been the public comment abandoned listing plans in favor of large-scale investment to buy the competition 'in the past two years, if the pursuit of profit, they would have to sacrifice the size of the market so the public comment share will be affected. 'Tao said that the current public comment on it, do not buy the business profit is mainly include input end of the phone. And buy the industry's Air Jordan CP3 VI Mens Shoes domestic competition has become increasingly clear, we are beginning to pursue profitable direction. In addition, the company has some basis in the Nike Zoom KD VII 7 mobile terminal R \u0026 D investment, it will not have too substantial growth, 'the company expects Nike Air Nike Air Mission Classic Mens Shoes Presto Mens within a year could reach profitability and profit next will become an increasingly important goal.' Tao said that based on the information, benefits and services of the layout, the Nike Flyknit Trainer public comment has entered the healthy development track public comment yesterday also announced that its mobile client unique users will exceed 40 million, compared with 10 million last year, an increase of three times more. By the second quarter of 2012, Air Jordan XIII 13 the public comment number reached 1.5 million businesses. It is worth noting that in June this year, the views from the end of the public comment over mobile PC Yelp end financial results, the company's second-quarter revenue rose 67%, net loss of $ 2,000,000. Yelp second quarter despite losses, Designer Shoes performance is still better than market expectations. Compared with the high point of the year the stock has fallen nearly half. Yelp is expanding European market, currently operates in 90 countries worldwide.


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Company Overview of Greater Kansas City Chamber Of Commerce
Executive Profile
James A. Heeter
Chief Executive Officer and President, Greater Kansas City Chamber Of Commerce
|Total Calculated Compensation
|This person is connected to 2 Board Members in 2 different organizations across 2 different industries.
See Board Relationships
Mr. James A. Heeter, also known as Jim, serves as the President and Chief Executive Officer of Greater Kansas City Chamber of Commerce. Mr. Heeter is a Managing Partner of the Kansas City, Missouri office. Mr. Heeter served as a Managing Partner of Sonnenchein Nath & Rosenthal's Kansas City office. Mr. Heeter specialized in Corporate Securities and Healthcare. He has been an Independent Director of American Italian Pasta Company, Inc. since May 2000. He has been a Trustee ...
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Independent Director, Chairman of Nominating & Governance Committee, Member of Audit Committee and Member of Strategy Committee
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Home > News > Nation
Thursday June 20, 2013 MYT 7:30:00 AM
Friday July 12, 2013 MYT 1:34:26 PM
by kang soon chen
PETALING JAYA: Schools have the discretion to close immediately once the Air Pollutant Index (API) reading reaches the hazardous level.
Education Ministry director-general Tan Sri Abd Ghafar Mahmud said Thursday the National Haze Action Plan had been amended to allow the Education Ministry to take the necessary action to safeguard the health of the students in areas affected by the haze.
"The school authorities have to constantly monitor the haze situation by checking the API reading available on the Department of Environment website," Abd Ghafar said in a statement on Thursday.
He added that schools in areas with API reading exceeding 300 (hazardous level) have to be closed immediately, he added.
"If the API reading is more than 250, the school authorites have to stop all co-curricular and outdoor activities.
"The school authorities also have to cease all outdoor activities involving high-risk students who may be affected by the haze once the API reading is higher than 150," said Abd Ghafar.
He advised students to wear nasal mask and drink a lot of water.
"The school authorities have to send students who have fallen ill to the clinic or hospital nearby.
"Parents who are concerned about the health of their children are given permission to take their children out of the school as long as the school is informed," said Abd Ghafar.
About 200 primary and secondary schools in Muar have been given a directive to close on Thursday and Friday in view of the API reading that touched 337 in the area on Thursday.
Related Stories:
Haze: Flights not affected
Haze: Muar's 200 schools to close temporarily from today's afternoon session
Haze: Muar's air quality turns hazardous; many private kindies close
East coast hit by the haze, too
Don't go out to Straits of Malacca at night'
Four times as many hotspots in Sumatra now
Many taking precautions against haze
Peat fires and the ever-repeating haze
Haze: Unhealthy air quality in Pasir Gudang, Larkin Lama, Kota Tinggi, Kemaman
Asean ministers to discuss haze problem
Tags / Keywords:
Haze, School, air, quality
Park visitors using various methods to minimise the effects of the unhealthy air quality
Liow: More direct flights to China to promote tourism
Singapore banks urged to curtail loans to haze-linked firms
Experts: PM2.5 gives better picture
Indonesia seeks help over fires
Ali Tinju's threat knocks out Taman Melaka Raya
Orang asli girls weigh about 15kg but in stable condition
Man claims MH370 wreckage on south Philippines island
TPP may cause Malaysian farmers to lose rights, claim activist groups
Make sports a culture, says Najib
Chong: Certain politicians responsible for fanning racial sentiments
Catriona still going strong in the Tour at 46
Facebook enhances tools for TV producers to engage fans
Revving it up in Rawang
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Chief Joseph Pass
|Chief Joseph Pass
US Forestry sign at Chief Joseph Pass
|7,251 ft (2,210 m)
|Traversed by
|Lemhi County, Idaho / Beaverhead County, Montana,
United States
|Bitterroot Mountains,
Bitterroot Range,
Rocky Mountains
|45°41′05″N 113°55′59″W / 45.68472°N 113.93306°WCoordinates: 45°41′05″N 113°55′59″W / 45.68472°N 113.93306°W[1]
Chief Joseph Pass el. 7,251 feet (2,210 m) is a mountain pass on the continental divide of the Rocky Mountains in the northwestern United States. It separates Lemhi County, Idaho and Beaverhead County, Montana. The pass is in the Bitterroot Mountains and is traversed by Montana State Highway 43. The pass is named after Chief Joseph of the Wallowa band of the Nez Perce tribe who traversed the pass in 1877 during the Nez Perce War.
West of the pass, the highway connects with US-93 at Lost Trail Pass, about .5-mile (0.80 km) west of the divide. Between the passes the Montana highway is actually in Idaho, for about 1-mile (1.6 km).
This is the northern most pass on the continental divide between Idaho and Montana. Less than a mile north, the Continental Divide heads eastward into Montana, following the Ravalli-Beaverhead county line towards Butte. East of Chief Joseph Pass, Highway 43 descends to Wisdom 26 miles (42 km) away passing the historic Big Hole National Battlefield of 1877. In early September 1805, the Lewis and Clark Expedition either crossed this pass or the nearby Lost Trail Pass as they traveled westward into the Bitterroot Valley of present-day Ravalli County.
The Lost Trail Powder Mountain ski area is about 1-mile (1.6 km) west, at Lost Trail Pass on US-93.
See also[edit]
External links[edit]
- Idaho Transportation Dept. - webcam of nearby Lost Trail Pass - US-93
- Lost Trail.com - Lost Trail Powder Mountain ski area - official site
|This Idaho location article is a stub. You can help Wikipedia by expanding it.
|This Montana state location article is a stub. You can help Wikipedia by expanding it.


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Case Study | April 21, 2014
rbTech Raises The Operational Bar WIth RMM SolutionSource: N-able Technologies Inc.
"If you don't evolve, you don't succeed," says Rubin Bennett, owner and founder of rb Technologies (rbTech), an IT services firm with customers throughout the New England region. With more than 16 years in the business, Bennett knows what it takes to grow and thrive even as new advances drive change in the fast-paced technology industry.
Located in East Montpelier, Vermont, where small business is the name of the game, rbTech opened its doors in 1997 and employs a staff of six, including five engineers.
In 2012, Bennett sensed a need to become more proactive – instead of reactive – as clients began to express an interest in more robust IT services to protect and optimize their businesses. Bennett knew that remote monitoring and management (RMM) technology could get his company where it needed to be, offering a foundation for more efficient operations and a better way to build a managed services practice.
Download the full case study below to learn more.
N-able Technologies Inc.
MORE FROM N-able Technologies Inc.
Contact Details
Ottawa , ON K2K 3K2


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Elle Macpherson Intimates' light-blue lace bra features underwired, lightly padded cups for subtle support. Complete a pretty everyday set with the matching briefs.
Light-blue and off-white laceBalconetteLightly padded underwired cupsAdjustable elasticated straps, bow, stretch-mesh wingsHook fastenings at backFabric1: 100% polyamide; fabric2: 85% polyamide, 15% elastane; lining: 100% polyesterMachine washDesigner color: Heather Haze/ Retro Cream
Product code: 340033 - Need help? Contact us


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- OSI-Approved Open Source (3)
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Henry E. InsleyAmerican (1811 - 1894)
Untitled [Charles B. Curtiss before Leaving for California]
ca. 1849photograph | daguerreotype
Not on view at this time; find out where you can see works from our collection at locations around the Bay Area while our building is closed for expansion
3 11/16 in. x 3 1/4 in. x 9/16 in. (9.37 cm x 8.26 cm x 1.43 cm)
Acquired 1999
Collection SFMOMA
Purchase through a gift of Lucinda Watson, the Evelyn and Walter Haas, Jr. Fund, an anonymous gift, H. Marcia Smolens, Tony and I'lee Hooker, and the Accessions Committee Fund


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Gramophonedzie at Festivals
Gramophonedzie (born Marko Milicevic) is a Serbian DJ from where he also studied Sound Design at the Belgrade University. His passion for funky music, live instruments and intensive vocals has resulted in a couple of songs released under the name "Gramophonedzie". He started his career in the year 2000 as participant at the Irish RedBull Music Academy. In 2006 he composed the theme tune for Veliki Brat, Serbian version of popular TV reality show Big Brother. Read more on Last.fm.
Similar Artists
Jazzbit , Shemian , Jamie Berry , AlgoRythmik , The Correspondents ,
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House , electro-swing , dance , trip-hop , trip hop ,
- Exit Festival 2011 - (Unspecified Stage)
- Exit Festival 2011 - (Dance Arena)
- Creamfields 2010 - 28 Aug 2010 (Mixmag) Less...


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An open letter to my fellow South Africans: I am ready. Are you?
- Jay Naidoo
- 08 Feb 2013 01:26 (South Africa)
Tomorrow it could be Your Mother, Your Wife, Your Sister or Your Daughter. Sexual violence knows no race, religion, status or class. It affects us All.
The question we all ask, "When are we going to confront the horror of sexual violence that leaves a bloody stain on our social fabric?"
As men we need to stand up and take a stand. We need to stop abusing, raping and hurting our women.
The solution lies in us, men
Have we forgotten that it is from the womb of our Mothers that we experienced life for the first time? It was her blood that brought us the love and the food that made us grow. It was from her swollen belly that we emerged to take those first gasps of air and bleat our first cries. It was on her breasts that we suckled the milk that comforted and fed our infant lives.
How could we violently tear those precious memories and brutalise the foundation of our hopes, our aspirations and our futures. Have we forgotten our Mothers who put food on our table? How often they would be content with a cup of tea and buttered bread because there was not enough to go around. Have we forgotten that first day, and every day after, that she prepared you for school, ensured you had done your homework and were safe in this troubled world.
Now is the time to pay back that debt
I hear the calls for more stringent penalties. I heard the impassioned and angry calls for the return of the death penalty. Yes, we need a criminal justice system that works efficiently. We need more specialised policing that sees the woman who is raped as the victim, who needs society's protection and comfort. Yes, we need that more urgently than ever.
But we need to look at the root causes. Men are not born as rapists. Something happens that make these men violent and vengeful against women. Our role as parents is the biggest influence on what our children will become. That cannot be outsourced to government. The home is the place where we learn love, compassion, tolerance and integrity. The home is where we are taught respect for each other, the ability to listen with humility and to learn from our mistakes.
Gender equality needs to start in the home. Respect for the rights of girls and women begin in the home. And men have to learn that a real man's place starts off with changing napkins, preparing the family meals and playing a role in raising their children.
What happens in our homes happens in our society. Abuse has to be stopped in our homes, even if it means denouncing your husband, son, brother, uncle or grandfather.
We often say to our boys, "Stop crying? Be a man." How often do we do we teach them that, "You are different from your sister." And then we buy them realistic "guns" because a man has to show his power. And our girls we package into "Barbie dolls" and prepare them for lives to be served as commodities for the whims of the fashion market and the pleasure of men.
Dress that up in patriarchy and tradition, implant a brutal impact of migrant labour that has broken the family fabric for generations, immerse that in the growing poverty and inequality that bedevils us and we have a toxic Molotov Cocktail that explodes around us.
We need to go back to the drawing board. One in every two South African women will be raped or abused in her lifetime. That is Your Mother, Sister or Daughter, Aunt, Grand-daughter or Cousin.
We cannot have a policeman or woman in every street. We need to watch over each other. We need to take our streets back from the criminal minority. That means a painstaking approach to building grassroots organisations that work with the community policing forums.
I undertake to attend the next meeting of the community policing forum in my area and to work with the local police to ensure our streets are safe.
I undertake that I will offer my help and support to media houses, NGO's and social movements that want to work on a roadmap to build a tsunami of action that involves our people in resisting the heresy of violence against our girls and women. Can we have our faith- based and business leaders take a lead?
I undertake to work with organisations such as Section 27, the TAC and Equal Education, which are at the forefront of fighting the epidemic of sexual abuse and horror that thousands of our girls faced daily in our schools. And that means tackling the disease of "bullying" that dominates the playgrounds of so many of our schools.
We live in a new digital world today. Almost everyone has a cellphone. It is the most pervasive platform in the world. Applications can be designed for every possible option. Can we bring together social movements, NGOs, developers, funders, business, government, the SAPS and media to design the applications that allow us to target the geographic hotspots, the individuals involved and the results of actions taken by the criminal justice system?
Why is there not a blacklist of offenders which weeds out sexual offenders in our schools? How many times I have heard from hardworking teachers who have forced out sexual offenders parading as teachers in their schools. But they lurk within the system and we as parents are blissfully unaware that the people in whose trust we leave our innocent girls, are continuing to violate them.
What we need is a massive campaign. Now. The number of victims mounts daily. The psychological and physical toll and economic costs are incalculable. We need our political leadership today. Words are not enough. Lead by your actions. You will find us citizens of South Africa willing to follow bold action to secure the human rights guaranteed by the Constitution. Women have to be equal partners in building the democracy we fought for.
We owe it to Anene Booysen. We owe it Corlia Olivier, who said on SABC, "My child almost looked purple. She was in such a bad state. All her fingers were broken, her legs were broken. Her stomach had been cut up, you could see her intestines. Her throat was also slit open."
We owe it to the 360 women who will suffer the same fate until this days is over, another 360 tomorrow and every day after. And the tragedy is that this is only a tip of the iceberg.
The time has come to act. Will a million real men stand up and be counted tomorrow? That will be our real test of our democracy.
I am ready. Are you? DM
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- Protests, police and cowardice – our State of the Nation
- New stones for my Madiba rosary
- The final journey and the legacy that will always live in our hearts
- After the tears, the hard work of building the world that Mandela believed in
- Mandela's gone. But he will be with us, forever.
- Bekkersdal: The turning point in SA municipal politics – time for a line in the ground
- Africa Rising? Whose Africa?
- The scramble for the Arctic and the dangers of Russia's race for oil
- Africa's future is clear: Youth, Technology & Broadband
- Child mortality is our human rights failure of the 21st century
- Technology can wipe out the cancer of corruption
- My open letter to South Africa
- Amputating the soul of our children
- The vision of the Invisible Children
- A humble billionaire, asking tough questions
- Cry, the beloved country; cry, the beloved federation
- Humanity at a crossroads: Fighting for climate justice
- Wanted: Ancient wisdoms to heal our planet
- The taste of power: its sanctity and its perversion
- When the town I loved burned down, or, when Heaven was visited by Hell
- As our Constitution lives, so does Mandela
- Bangladesh: Losing some battles, but winning the war
- Rana Square – the Ground Zero of workers' rights
- Small-scale farming: simple, successful, sustainable
- A global debate needs local voices
- When will Africa be led by the needs of its people?
- The faultlines in our society: Why are we so angry?
- Nigeria: Africa's best hopes and worst fears
- Our ancient African heritage holds the key to our future
- To build a better world for all, we need a new narrative, new energy, new commitment
- A culture of service and tolerance: Lessons from Chris Hani
- Open data platforms: a tool to revolutionise governance
- Aluta continua: Why the fight for quality healthcare can't be over
- 'I raped her because she belongs to me'
- Would Hani and Slovo today be accused of Neo-liberalism and Counter-revolution?
- An open letter to my fellow South Africans: I am ready. Are you?
- A trip to Limpopo: The Forgotten Land
- 'I have a right to a toilet - it's human dignity'
- Matric pass rate: On the road to Nobody
- The challenges of today are South Africa's opportunities of tomorrow
- India: The ongoing tyranny of the caste system
- To my generation: Listen. Listen very carefully.
- The Lula moment and this country of ours, South Africa
- African youth: Fulfilling the potential
- Africa's 'leadership crisis' - we have more agency than we think
- Think climate change isn't your problem? It will be when you can't eat
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- Madiba week: The lessons his sacrifice taught us, part II
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- iMaverick, Monday 28 November
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Kindler syndrome
- Bullous acrokeratotic poikiloderma of kindler and weary
- Congenital bullous poikiloderma
- Congenital poikiloderma with bullae, Weary type
- Hereditary acrokeratotic poikiloderma, Weary type
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American Society of Gene & Cell Therapy (ASGCT) 18th Annual Meeting, Wednesday, May 13, 2015 - Saturday, May 16, 2015
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VI International Conference on Rare Diseases and Orphan Drugs (ICORD 2010): Global Approach to Accessibility in Rare Diseases, Orphan Drugs and Neglected Diseases , Thursday, March 18, 2010 - Saturday, March 20, 2010
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- Bullous acrokeratotic poikiloderma of kindler and weary
- Congenital bullous poikiloderma
- Congenital poikiloderma with bullae, Weary type
- Hereditary acrokeratotic poikiloderma, Weary type


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See All
My Communities
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What really sticks out to me is his attitude about the fingering: in a relationship based on mutual respect and reciprocity, he would be happy to be able to make it great for you. (As a man, this is the first I've ever heard a man treat touching a woman's genitals and bringing her to orgasm as some sort of chore.)
Here's my advice: the condoms are non-negotiable. If he continues to throw a selfish tantrum over it, consider ending the relationship.View Thread
The fact that you don't find Jackass or The Hangover funny doesn't mean you've lost anything. Those kinds of shows are designed to make 14 year-old boys laugh. I'd be far more worried if you did find them funny.
The fact is, we change constantly over the course of our lives. We're never the same person that we were a year ago or 5, 10, 20 years ago. Our experiences change us.
In terms of relationships, we're all self-centered to an extent: we expect the relationship to meet our needs. If we invest in the needs of our partner, it's at-least partially because we expect a return on that investment--we expect them to be attentive to our needs.To use Gary Chapman's language, if you help keep your partner's love tank full, then they'll have the fuel they need to do the same for you.
Roh, perhaps you'd enjoy doing something 100% for others, like volunteering at a soup kitchen, or whatever does it for you. I find it liberating to sometimes devote myself entirely to a task that is not about me at all.View Thread
"You might also find it interesting to know that the partner most in control of how often a heterosexual couple has sex is the man."
I'm sure you meant to say, "...most typically in control...," right?
This certainly goes against conventional wisdom. Most married men I know (including myself) seem to believe that it is their wives who are the "deciders" in this regard.
I've always figured that the lower-libido partner holds most of the cards in this negotiation.
Could you expand a little what you mean by this?View Thread
I grew up in Michigan--where I live now (Kansas) definitely gets a lot less snow, but we've been getting it pretty steadily this winter--a few new inches every time the last snow is in danger of melting. We got another 3 inches or so last night.
View Thread
She has actually never admitted not enjoying sex anymore, although it is perfectly obvious that she doesn't. She's kindof an odd case that way--it's very hard to get her to open up about anything.View Thread
We've tried date nights before, we've tried counseling a couple different times. We went away for the weekend last summer--it was nice, but nothing changed long term. I think the thing is, she's perfectly OK with the way things are, it appears. We don't really have a romantic life--we love each other and care about each other, but we kiss more or less the way I kiss my mother. We're like room mates who have very boring sex once or twice a month.View Thread
We rang in the new year with sex--the most terrible, unsatisfying lame quickie sex I've ever had.
How do you have this conversation with your wife of 26 years--that after years of being frustrated by how infrequently we had sex, I'm now even more frustrated by the rare occasions when we do have sex? She's basically finally succeeded in completely eradicating my desire to have sex with her.
Her idea of sex: she comes to bed naked and says, "are we going to do it?" Then, I have to pry her legs apart. A couple minutes of foreplay might be grudgingly tolerated, then we get it over with, the more quickly the better. She surely doesn't care if it's satisfying for me, but she also doesn't seem to want it to be satisfying for her, either. I've told her many times what would make sex more fulfilling and appealing to me--she's simply not willing to lift a finger to achieve those goals.
I, on the other hand, am more of a pleaser personality. My enjoyment of sex is determined about 95% from whether I am able to make it satisfying for my wife. But she doesn't allow that, either. She actively prevents any kind of touch that might lead to pleasure/orgasm for her.
We had a rough stretch in our marriage a few years ago, but things have been a lot better in the last couple years--outside of the bedroom, anyway.
I just feel lost about this. I'm not ready for a life without sex (I'm 49 years old), but I can't stand her idea of sex, either. I really don't want to divorce, and cheating is not an option for me.
If I told her today that I'm not interested in having sex with her anymore, I doubt that she'd be too upset about that. (About not having sex anymore, I mean. I'm sure she'd be upset and feel rejected about me saying I don't desire sex with her anymore.)
I've tried talking to her about this many times in the past. I've read books and suggested that she read them (she doesn't). We're a gender-role-reversed couple in some ways, one of them being that I'm the only one who ever wants to talk about the health of our relationship.
I just really don't know how to take the next step. I'm feeling pretty lost.View Thread
It's not all that different from mismatched libidos, where the lower libido partner has all the power in the sexual relationship. A partner who, say, decides to lives as messily as s/he likes (after all, if their partner wants the house neater, s/he can make it neater), or who imposes their high standards of tidiness on their partner without compromise, is sending the message that their partner's preferences and desires are unimportant to them.
No one likes receiving that message.View Thread
My office is fairly untidy, but it is a working space (relaxation is not the goal), and it is NOT a shared space.
At home, it's the opposite. I keep things tidy because clutter is a stressor and because it is a form of courtesy to the people with whom you share the space.
For those who are the less neat partner. I'm far from a neat freak, but a cluttered house stresses me out. It's hard to relax in a cluttered living room. So try to keep that in mind of your partner wants you to be tidier. It's stress-reducing for them.View Thread
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It's that time of year again. And thanks to the people at Fulton Bank (This year's major sponsor) it just might be the best Restaurant Week… ever. Sure, we're biased to our local roots, but can you blame us?
Foodies can rest easy with budgeting – Meals will range in price from $5 to $30 depending on your flavor. The goal of restaurant week is to expose York as one of the top foodie destinations in the Susquehanna Valley, and to improve economic conditions in the process.
Some restaurants participating in the foodie fun are White Rose Bar & Grill, The Left Bank, Maewyn's, and Nuts About Granola. A full list of participating Restaurants, Pubs, and other local businesses is on the official Restaurant Week website.
The event runs from February 22nd through March 1st.
Want some cash for your favorite charity? We'll add $5 to the pot every time someone mentions @YAFCA and uses the #caredabout hashtag on Facebook or Twitter, including a location and photo (see our tweet here – we made our own as a friendly little example). The giveaway begins on February 22nd and runs through March 1st*.
We will announce the winner in a post right here on the blog on Monday, March 25th.
As usual, the entries will go on paper entries, and then the winner drawn on video. Now would be a great time to drum up some support for your favorite charity. Grab some friends and supporters and tell them to be sure to frequent Restaurant Week, and to tweet or post (on Facebook) their favorite dishes mentioning @YAFCA and using the #caredabout hashtag (featuring their favorite dish in a tweet or post).
There is a $100 max for this Social Giveaway.


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- Thursday, January 29, 2009
Ant Farm Media Van v.08 (Time Capsule)
Date + TimeThursday, January 29, 2009
7:00 p.m. - 7:30 p.m.
LocationFourth-floor galleries
During the exhibition The Art of Participation: 1950 to Now, museum visitors have been plugging in their own personal electronic devices (such as cell phones, cameras, MP3 players, and PDAs) into the media van's hookah-like console, which then copied and stored at random a digital audio or image file from those devices. Approximately 4,000 files make up this digital time capsule, which will next be accessed, or "opened," the year 2030.
For this special ceremony, join Ant Farm artists Chip Lord, Curtis Schreier, and Bruce Tomb for the official sealing of the Ant Farm Media Van v.08 (Time Capsule).
Chip Lord, artist
Curtis Schreier, artist
Bruce Tomb, artist
Admission + Ticketing
Free with museum admission.


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I love Nicholas Sparks, including his movies. Right now add The Lucky One DVD+UltraViolet Digital Copy for only $8.49, regularly $19.94, to your movie collection!
This deal ships free with Super Saver Shipping when you purchase $25 or more worth of participating products. For more ways to save, cash in your Swag Bucks for a FREE Amazon gift card.
More Ways to Save:
- Deals on Amazon
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Campus Tours
How to become a student
Shoreline is known for its beautiful campus. Outdoor areas are generally open to the
public to walk through and experience the inviting environment. Some buildings are
also open to the public during business hours.
Guided tours that offer a more in-depth view of the college are available with a confirmed tour reservation. To request a guided-tour reservation please contact:
Amy Stapleton
Guided tours are popular and reservation requests should be made at least 24 hours in advance. Also, please indicate any special accommodations that may be needed.
Thanks, and we look forward to seeing you soon!


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No-lift shifting and 2-step launch control. Both of these features are completely user-programmable using the WOT Box Graphical User Interface.
The major benefits are:
Price: $199 with FREE Shipping
Shipping costs are as follows and we be automatically applied at checkout.
All orders are shipped USPS Priority Mail with delivery confirmation. You will receive a tracking number at the email address used during checkout once your order ships.
Included in the kit
Compatible Vehicles
If your vehicle is not on this list, contact us at email@example.com to find out if the WOT Box will work on your car.
What is the WOT Box?
The WOT Box is an electronic device that makes WOT shifting easy by reducing your engine's power for a very short timed period while you shift. It allows the synchros in your transmission to mesh easily, reducing the chance of missing a gear and damaging your transmission. If the input shaft on a manual transmission is not allowed to slow down enough it will lock you out of gear time after time. We have developed a solution to the problem by controlling the ignition at the shift point to ease the transition from one gear to another.
What is WOT Shifting?
WOT shifting is a common practice used by drag racers regardless of if they are running turbo, supercharger or naturally aspirated. WOT shifting is accomplished by holding the gas pedal to the floor and never "lifting" off the gas during the shift. The problem with doing so is when you depress the clutch engine RPMs flare up and a brief amount of time is required for the transmission input shaft to slow down. Even the most successful drivers have been known to miss a gear occasionally. It requires complete concentration and lightning quick reactions in order to pull it off.
"Keep the pedal to the metal, blip the clutch, and grab the next gear. Do it fast enough before the redline, and your reward will be a zero lag, constant boost shift."
When you WOT shift, your engine is still producing peak power through the shift. With the clutch released, the engine revs up. This increase in RPMs and the full output power of the engine makes it very difficult for the synchros in your transmission to engage. The result means that WOT shifting can be very difficult and potentially damaging to your transmission.
How does the WOT Box make WOT Shifting easy?
Our WOT Box momentarily disrupts the engine power long enough for you to make an effortless, fast WOT shift. The result?
You can see the WOT Box in action by clicking on this page.
How does the WOT Box work?
The split second that you place your foot on the clutch pedal with the throttle all the way down, a signal is sent to the WOT Box that a shift has been initiated. When the module sees the signal from the clutch switch it will reduce engine power by cutting the ignition. The ignition control is a precise timed event that the user has complete control of. The box is capable of controlling ignition from 25 milliseconds to 700 milliseconds in 25 millisecond increments. With a range like that the wot box can even make Mr. Magoo look like a seasoned pro!
The drivers in our test cars describe using the WOT BOX as sitting in the car and going through the gearbox with the engine off!! All the drivers were amazed at how smooth the gearbox was. The transition from gear to gear is effortless and the boost gauge would barely show a blip between the shifts. We were able to test on an Evo8 with a laggy 60-1 turbo and managed to hold 30+ psi through every gear! Even on a stock turbo equipped Dodge SRT-4 we were able to knock 3 tenths off e.t. by simply hooking the box up. Our big turbo SRT-4 cars were able to drop up to 7 tenths off their E.T. with the WOT Box alone!
Will the WOT Box affect normal driving?
No. The WOT Box will only activate when you are WOT Shifting. Normal shifts will be ignored and continue to work just as you expect. You won't even know its there, until you make a WOT Shift!
How about some testimonials from users?
Visit our See It In Action page for testimonials, videos and more!
Will I be able to program it or do I need an engineering degree?
Many after-market electronics are bulky and full of dip switches, buttons and dials that are enough to make the average DIY'er bang their head on the wall in frustration! The WOT Box is simple to program with only one button and one LED. Check out our User's Guide to see how it is done.
Will the WOT Box work on a car that has the X brand ignition?
Yes. It will work on any ignition setup that has a common positive lead for all of the ignition coils. See our installation instructions page for custom wiring diagrams for specific ignition setups. If your setup is not listed, email us!
What is involved in hooking it up?
No new hardware is needed. The installation will only involve hooking up a few wires. Full instructions are available here.
Do I need a clutch switch?
No, you don't! Most recent cars have the clutch up switch already built in. If you have cruise control, you likely already have the switch you need. Contact us if you have questions.
How big is it?
It's small enough that you will be able to hide it up in your dash. The exterior dimensions are 3.8" x 1.6" x 0.8". Here is a blueprint of the enclosure we use. 1551KFL.pdf
When will my order ship?
We have units in stock and are shipping orders daily!
Optional Equipment
WOT Box AC Adapter - $16.99 - Free US Shipping
WOT Box Window Switch Add-On - $65 - Free US Shipping
The add-on can drive up to 15A solenoids directly. Contact us if you need additional capacity. See the installation instructions for details on the wiring.
WOT Box Wiring Harness - $16.99 - Free US Shipping
WOT Box Feature Upgrade - $70 - Free US Shipping


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Ann Fronczek
(PhD, RN)
Assistant Professor
Decker School of Nursing
Binghamton University
Phone: (607) 777-6164
E-mail: firstname.lastname@example.com
Ann Fronczek earned a BSN from West Chester University of Pennsylvania, an MS degree with a concentration in Family Nursing from Binghamton University and her PhD. at Villanova University. The title of her dissertation was "The Lived Experience of the Primary Family Caregiver for a Person with Head and Neck Cancer"
Research and Practice Interests:
- Telehealth
- Nursing Education
- Oncology Nursing
- Caregiver Concerns
Areas of Teaching:
- Nursing Education
- Qualitative Research
- Honors Program


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Rookie idol group EvoL returns this week with their second mini-album, an MV and a question: do you think you can handle them? Lead single "Get Up," one of Second Evolution's two new tracks, demands that you step your game up if you plan on giving chase.
Short 'n sweet at 2:49, the atypical progression of hip-hop/R&B/pop track "Get Up" matches with EvoL's desire to stand out from the sea of idol groups and also gives fans a break from the "boring development" of today's pop songs. (We gotta agree with LOEN on that jab — K-pop tracks do kind of follow a formula these days, don't they?)
Check out Jucy, J-Da, Say, Hayana and Yul showing off confidence and personality in the "Get Up" MV below:
Second Evolution Tracklist
01. "Get Up"
02. "Love"
03. "Magnet" [Previously Released]
04. "I'm Sorry" [Previously Released]
05. "Get Up (White Dress Remi)"
06. "Get Up (Instr.)"
Do you think EvoL's "Get Up" successfully deviated from 'typical K-pop?'


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The Color Run - London
Runners celebrate in the Festival Area after the Color Run presented... News PhotoBestof,Bestpix,Celebration,England,Horizontal,London - England,Planet - Space,Running,Sport,The Color Run,Topics,Topix,Track And Field,UKPhotographer Jordan MansfieldCollection: Getty Images Sport 2014 Getty ImagesLONDON, ENGLAND - JUNE 01: Runners celebrate in the Festival Area after the Color Run presented by Dulux, known as the happiest 5km on the planet on June 1, 2014 in London, England. Runners of all shapes, sizes and speeds start wearing white clothing that is a blank canvas for the kaleidoscope of colours they encounter around The Color Run course. At each kilometre a different colour of powder is thrown in the air with the runners becoming a constantly evolving artwork. At the end of the course runners are greeted by the Colour Festival where the air is filled with music and stunning coloured powder bursts creating a vibrant party atmosphere. (Photo by Jordan Mansfield/Getty Images for Dulux)


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Photo by Hans Gissinger
Leave a Review
yummm... ths is my new favorite rib marinade. I thought it was too salty with the dipping sauce, but liked the tange added by the lime and white pepper. Next time I will probably just add some white pepper to the marinade and a squeeze of lime at serving instead of using the dipping sauce. My husband loved it just way it was, though.flag if inappropriate
Very simple tasty marinade! Used it on pork chops and they came out nicely caramelized on the surface but very moist on the inside. Only change I would make is to double the ginger next time as I did not get a strong ginger flavor in the finished product. Dipping sauce quantities per person were a bit wasteful. I served half the called for amounts to each person and there was still a lot wasted. A little bit goes a long way with this dipping sauce since the recipe already has plenty of salt in it.flag if inappropriate
Fantastic marinade. The cooking times are off though.flag if inappropriate
I have now made this twice. The first time I followed the directions and the ribs came out a little salty. This last time I made the following changes. First I doubled the recipe, except as follows. Still only used 6 cloves of garlic they were large cloves. Used about 1/2 tablespoon of kosher salt and I added some juice from a pineapple that I had just cut up and the core was thrown in the food processor to add some more sugar and acid. They marinated for about 10 hours and they taste greatflag if inappropriate


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On Sunday, youth from across the community unite for one cause.
The Mexico Mission auction will feature both, a silent and live auction, designed to raise funds for homes to be built during spring break in Mexico.
The workers will include members from the United Church of Los Alamos, the Unitarian Church and non-church members, all who are on a mission to help their fellow man.
The students have donated their time and gift certificates for services. The items include tennis lessons, babysitting, a photo session and more.
The process wouldn't be possible without UNCHLA member Laura Erickson and husband Randy.
"I am excited about the auction because it brings the whole community and church family together to support the mission trip," Laura said. "It is a very tangible way for everyone to say they support the youth and adults who are going to build in Puerto Peñasco."
The United Church of Los Alamos heads out on trip number 28 this year with an equal number of adults and youth — experienced and freshmen trip-goers.
Just prior to the auction, the team often finds out information on the families they will build for. Amor Ministries works with local Pastors of Puerto Peñasco to decide who will receive the gift.
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Click the question mark below to see where your account ID appears on your mailing label.
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RICHMOND, Va. (April 12, 2012) Virginia Commonwealth University School of Medicine researchers have discovered that changes in the gene expression of a key enzyme may contribute to high blood pressure and increase susceptibility to forming blood clots in pregnant women with preeclampsia.
These findings could provide clues to the best treatment approaches for high blood pressure and the formation of blood clots that can block blood flow to a pregnant woman's internal organs and lead to organ failure.
Researchers have been working to determine the root cause of preeclampsia on the molecular level and have now identified that epigenetic mechanisms may be at play. Epigenetics refers to changes in gene expression that are mediated through mechanisms other than changes in the DNA sequence.
In a study published online this week in Hypertension, a journal of the American Heart Association, the VCU team reported that thromboxane synthase an important inflammatory enzyme is increased in the blood vessels of expectant mothers with preeclampsia. The thromboxane synthase gene codes for this enzyme, which is involved in several processes including cardiovascular disease and stroke. This enzyme results in the synthesis of thromboxane, which increases blood pressure and causes blood clots.
"The present work is unique because it opens up a new concept as to the cause and subsequent consequences of preeclampsia relating to epigenetics," said corresponding author Scott W. Walsh, Ph.D. , professor in the VCU Department of Obstetrics and Gynecology . "It is the first study to show that epigenetic alterations in the blood vessels of the mother are related to preeclampsia."
According to Walsh, one of the main epigenetic mechanisms is methylation of the DNA, which controls the expression of genes. The increase of this enzyme in the blood vessels is related to reduced DNA methylation and the infiltration of neutrophils int
|Contact: Sathya Achia Abraham
Virginia Commonwealth University


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Eating disorders are serious, complex illnesses that affect more than 10 million Americans. The Harris Center at Mass General is dedicated to research, education and advocacy in eating disorders and offers clinical assessment and treatment for children, adolescents, adults and families affected by these diseases.
Fashion luminaries talk eating disorders at Harris Center forum
Dr. David B. Herzog, Natalia Vodianova, Michael Kors and Anna Wintour at the Harris Center Public Forum, "Health Matters: Weight and Wellness in the World of Fashion." Photo courtesy of Roger Farrington
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