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Photos of Mischa at the Fashion for Relief 2008 benefit at the Natural History Museum in London have been uploaded. This event is also held as part of London Fashion Week.
Currently being uploaded are photos from another event; Samsung The Red Thread.
Photos of Mischa at the Marie Claire 20th Birthday Party in Marylebone, London have been uploaded. Mischa is pictured in some of the shots with supermodel Jessica Stam.
Some additional low quality photos of Mischa at the Giles Spring/Summer 2009 Collection Show have been uploaded. Also added are some candids of Mischa and friends leaving Bungalow8 club after Marie Claire Party – Sep 16. Thanks to Marie for both sets of photos!
Some photos of Mischa at the Giles Spring/Summer 2009 Fashion Show during London Fashion Week have been uploaded. I'll endeavor to add more pictures as they become available.
A short video of Mischa being interviewed at the Temperley London Fashion Show can be viewed here. She talks about signing on to film Bhopal in November (which is the correct title from the news posted below). She also talks about how the new season of her handbags are due soon and talks about the kinds of designs featured in her upcoming line of headbands.
After taking in the Temperley London show on Monday, Mischa Barton kicked back at the designer's after-party at trendy members club Bungalow 8."I'm taking a bit of time out being in London seeing friends and family,"she said. Having just finished her second line of handbags and headbands for her collection with accessories designer Stacey Lapidus, Barton said she would start shooting a new film in November titled "Nepal" opposite actor Charlie Sheen. "I will be filming in India, which will be exciting," she said.
Via WWD Fashion
A stunning photoshoot by Matt Anker has been uploaded to the gallery with massive thanks to Marie. The photos were taken in December 2007, back when Mischa was promoting Closing the Ring.
Additional photos from Mischa's night out following the Temperley London fashion show have been added. These photos do feature Josh Harnett and have caused somewhat of a media field day already. It's been a while since Mischa has been involved in a made up love affair so let's ride this one out as well, lol. Thanks to Marie for these photos too.


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April 13, 2010
Lobos at Linda Estes Tennis Complex
Friday: BYU at Lobos, 2 p.m.
Saturday: Utah at Lobos, 2 p.m.
Sunday: TCU at Lobos, 3 p.m.
By Richard Stevens -- Senior Writer/GoLobos.com
It's fair to say that part of the reason Kristin Eggleston is a Lobo is because Michelle Heidbrink used to be a Lobo -- and not because Heidbrink is a Lobo.
Confused? Well, the recruiting story of Eggleston rolls out like this:
Lobo coach Roy Cañada was searching, well, the world looking for some young talent in which to build up his UNM women's program. He called up Heidbrink, a former Lobo player, and asked her if she had any future Lobos in her area code.
At that time, it was the Palm Desert, Calif., area code where Heidbrink was coaching high school tennis. Heidbrink -- not yet a UNM assistant -- already had been gently pushing one of her seniors (Eggleston) in the direction of Albuquerque.
"I watched her grow as a player and even had hit some balls with her when she was younger," said Heidbrink. "When Kristin started looking at colleges in her senior year, I said, 'Hey, what about UNM?'
"That was just me being an ex-Lobo. I didn't even have the job then. It wasn't even open then. I was just an ex-Lobo pushing my school. I got the job a couple of months after Kristin signed here."
Of course, Heidbrink did have a slight agenda in pushing Eggleston toward UNM. Heidbrink was planning to apply for the job. She figured if she landed it, it would be nice to coach Eggleston on the next leg of her tennis journey.
"Kristin is one of those people and players who goes above and beyond in everything they do," said Heidbrink, who came to UNM in June of 2009. "In high school, she was one of those players who was the first one to the court and one of the last to leave. She is just a super motivated girl.
"And she is so much fun just to be around."
Eggleston doesn't hide the fact that she is happy to be on the tennis court. She smiles a lot. She is known for her smile. Sometimes it even looks like she is smiling when she pounds a tennis ball back across the net.
That doesn't mean that Eggleston isn't a tough player. She has a few hard edges when the racket is in hand. The Lobo freshman, battling in the dog-eat-dog trenches of Division I tennis, is known as a fighter -- game by game, point by point.
In a season in which the young Lobos are being tested physically and mentally, it's a good trait to have.
"I feel like I'm a fighter on the court. I never really give up," said Eggleston, who is 9-24 as a Lobo. "I can come back because I won't give up.
"I probably play better when I'm behind. I seem to get a little tense when I'm up. When I'm behind, I play like I have nothing to lose and I'm more aggressive."
Eggleston's says her strength is on the base line. "I tend to stay back," she said. "I think I do a good job with different shots, mixing things in. I need to work on attacking the net more."
So far, it's been a challenging year for Eggleston and the Lobos, who are 1-17 on the season. "We haven't given up. We're still fighting," she said. "It's been a tough year, but we are doing a good job of concentrating and going out there trying to get better."
A big adjustment for many freshmen is balancing the demands of being both a student and an athlete. Eggleston had a few advantages in that department. She had a 4.3 GPA at Palm Desert High. She has a 3.8 -- so far -- as a UNM freshman.
"I love it here," she said. "I didn't think about playing time when I came here. I just had a good feel about the coaches and the players.
"But it's been a good opportunity to get experience. Now, I just need to work on getting better," she said, through a smile.
Editor's Note: Richard Stevens is a former Associate Sports Editor and sports columnist for The Albuquerque Tribune. You can reach him at firstname.lastname@example.com.

subset: commoncrawl


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The U.S. Supreme Court has asked the Obama administration for an opinion on whether the Montana Supreme Court ruled correctly when it determined that PPL Montana must pay rent for the company's use of riverbeds beneath its hydroelectric dams, media outlets reported.
The nation's high court wants acting Solicitor General Neal Katyal, the Obama administration's top attorney before the Supreme Court, to weigh in on the case. The move signals that the high court has taken an interest in the case and may eventually decide to hear it, reports indicate.
PPL Montana wants the Supreme Court to overrule a Montana Supreme Court decision from last March that ordered the company to pay $40 million in current rent, as well as damages for not paying rent for land its dams sit on from 2000 through 2007, plus even more in future rent.
The court decision means the land under the dams is like other public land that is rented out, such as to those who graze cattle or drill for oil.
PPL Montana said the state overstepped its authority and in August asked the U.S. Supreme Court to consider in the case. PPL said the Montana Supreme Court was wrong in claiming Montana ownership over 500 miles of riverbeds under multiple hydropower facilities.
Grey said the streambed case raises broad federal issues that go beyond PPL Montana's business interests and has implications for the rights of streambed users including hydroelectric plant operators, ranchers, irrigators, cities, dock owners and recreationalists.
For more hydropower news and information, click here


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- Filed Under
Melissa Moore likes nothing better than to "see light bulbs above my students' heads when they discover an answer or uncover a truth for themselves."
The light bulbs are metaphorical, but the flowers brought to Moore's fourth-grade classroom Friday were real. They were delivered by Indianapolis Public Schools Superintendent Eugene White, who surprised Moore with the bouquet and the announcement that she was the district's Teacher of the Year.
Students cheered as White, accompanied by reporters, walked into her classroom at School 70, a performing-arts magnet school on the Northside ...


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text: Vegetarianism Category page Create Classic editor Talk0 This page needs content. You can help by adding a sentence or a photo. Add to this page!orSearch for "Vegetarianism" Jainism "Assessment| Biopsychology| Comparative| Cognitive| Developmental| Language| Individual... Spiritual practice Vegetarianism Pages in category "Vegetarianism" The following 3 pages are in this category, out of 3 total. J JainismS Spiritual practiceV Vegetarianism Retrieved from "http://psychology.wikia.com/wiki/Category:Vegetarianism"


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Hold down Control (PC) or Command (Mac) key + mouseclick to select more than one option
|Avg. Dinner Entrée
|Greater than $25
|$18.01 - $25
|$12.01 - $18
|$7.01 - $12
|Less than $7
Posted by TXgirl on 12/06/2010
It was bitterly cold outside so we ducked into the Heartland Brewery for warmth and dinner. We ordered the chicken enchilada soup to start. Very disappointing. It tasted like Campbell's vegetable soup with added shredded chicken. There was no additional seasoning! No cheese or tortilla strips as described in the menu. My grilled chicken sandwich was ok. Again, no flavor to the grilled chicken but I liked the sourdough bread it came with. My husband had buffalo wings and said they were "decent." Next time, I will be better prepared for where to eat in that area so we can get a better meal.
Posted by SweetieM on 09/24/2010
Yesterday, I brought a group of 10 people to this Heartland Brewery after work. We were trying to get together since one of our colleagues is moving. It was around 6pm and there were at least 12 tables available. We were asked by the rude hostess if we would be eating. I said that we would be having appetizers and drinks and maybe some people would eat. She had to go check with a few staff huddled in the corner and came back to say that if we were not each ordering an entree that we would not be allowed to have a table. We asked to sit outside where there was again at least 10 tables available. We were told that we couldn't do that either, because there was enough room. I assumed that since the economy has hit restaurants pretty hard, you'd think 10 people wanting to eat and drink in your restaurant would be welcomed. Instead we were told we could sit in the back near the bar. We were not walked to the area, but instead the hostess pointed to where we could go. We had to pull tables together ourselves and had to walk back and forth from the bar each time we wanted to order food or drinks. I WILL NEVER return to this restaurant or any other Heartland Brewery after this experience. I think the hostess that was working should be given some customer service training and allowed to seat 10 hungry and thirsty patrons in the real dining room.
Posted by BH on 01/30/2009
The only reason I can see for someone to go here is that they are one of the many tourists in midtown who don't know better. The beer is not bad, but that's just about the only non-negative of this place. The food is pretty lousy, especially for what you pay. You can get better food at what on of the many deli/cafes in the area for half the price. The service was not all too impressive, then again, this place seems a little understaffed, so I can't really blame the servers.
Posted by Lo on 06/14/2007
This location is great for the ladies that just want to sit at the bar, have a few drinks and some laughs afterwork . The bartenders are especially catering and fun, the drinks are good and priced right. The food is good also and moderately priced. It gets a little crowded sometimes, but still lots of fun.
Posted by sml on 05/14/2007
It's a great place to go for a quick bite to eat if you're in the area and don't feel like searching for another restaurant. It can be packed at times though, especially with tourists visiting Radio City or Rockefeller Center. A little overpriced, but that's what you get with this area of the city!
Posted by mindy on 03/25/2007
I enjoyed eating here and go here everytime I visit the city!
Posted by Anonymous on 01/19/2007
Service is friendly and beer is great. You can get better bar food at any bar in NYC.


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Discussions Relating to Universal Reconciliation > Bible Verses Used to Assert Not All Will be Saved
1 corinthians 10
<< < (2/2)
--- Quote from: ded2daworld on November 27, 2012, 03:47:46 PM --- I agree with EW (as usual) I do have another perspective besides that tho'
"Often we use the old testament as an example towards spiritual truths in the new testament. so if they are physically destroyed by God, does this example teach that unrighteous will be spiritually destroyed?"
I believe that when we are consumed by the fire of Holy Father, our refinement and purification removes the dross of the sinful thoughts words and deeds we have done. A total sinner then would be born anew but would also have nothing left of the old life. (Much as aborted baby's or like children way to young to understand much of anything before they die.)
We, that are called the saints of God, will cast our good works at his feet and realize in totality that all true good works were the Holy Spirit working through us. How much of the sinners personality or soul will remain - I don't know. But Job said, "I know that one day I will see God, it will be me, in my flesh, and not another." so it won't be like a totally new creature in the next age in all respects but, in some ways it will.
--- End quote ---
I agree. I think newness refers to nature. I will still be I, but a new creature in Christ, transformed. My identity changed when I was born of the "incorruptible seed"(Peter), the "word implanted which is able to save your souls"(James), born, "not of the will of man nor of the will of the flesh, but of God"(John).
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Having duplicate keys in our schemas can hurt the performance of our database: They make the optimizer phase slower because MySQL needs to examine more query plans. The storage engine needs to maintain, calculate and update more index statistics DML and even read queries can be slower because MySQL needs update fetch more data to […]
Search Results for: show index statistics
When Does InnoDB Update Table Statistics? (And When It Can Bite)
An InnoDB table statistics is used for JOIN optimizations and helping the MySQL optimizer choose the appropriate index for a query. If a table's statistics or index cardinality becomes outdated, you might see queries which previously performed well suddenly show up on slow query log until InnoDB again updates the statistics. But when does InnoDB […]
Pretty-formatted index fragmentation with xtrabackup
The xtrabackup compiled C binary (as distinct from XtraBackup, which is the combination of the C binary and the Perl script) has support for printing out stats on InnoDB tables and indexes. This can be useful to examine whether you'd benefit from "defragmenting" your MySQL database with OPTIMIZE TABLE, although I have not determined firm […]
InnoDB TABLE/INDEX stats
In Released and new coming features I did not mentioned two additional INFORMATION_SCHEMA tables available in XtraDB: It is INNODB_TABLE_STATS INNODB_INDEX_STATS These table show statistics about InnoDB tables ( taken from InnoDB data dictionary). INNODB_TABLE_STATS is | table_name | table name in InnoDB internal style ('database/table') | | rows | estimated number of all rows […]
How (not) to find unused indexes
I've seen a few people link to an INFORMATION_SCHEMA query to be able to find any indexes that have low cardinality, in an effort to find out what indexes should be removed. This method is flawed – here's the first reason why:
CREATE TABLE `sales` (
`id` int(11) NOT NULL AUTO_INCREMENT,
`customer_id` int(11) DEFAULT NULL,
`status` enum('archived','active') DEFAULT NULL,
PRIMARY KEY (`id`),
KEY `status` (`status`)
) ENGINE=MyISAM AUTO_INCREMENT=65691 DEFAULT CHARSET=latin1;
mysql> SELECT count(*), status FROM sales GROUP by status;
| count(*) | status |
|Â Â Â 65536 | archived |
|     154 | active |
2 rows in set (0.17 sec)
mysql> EXPLAIN SELECT * FROM sales WHERE status='active'; # query 1
| id | select_type | table | type | possible_keys | key   | key_len | ref  | rows | Extra      |
| 1 | SIMPLE     | sales | ref | status       | status | 2      | const | 196 | Using where |
1 row in set (0.06 sec)
mysql> EXPLAIN SELECT * FROM sales WHERE status='archived'; # query 2
| id | select_type | table | type | possible_keys | key | key_len | ref | rows | Extra      |
| 1 | SIMPLE     | sales | ALL | status       | NULL | NULL   | NULL | 65690 | Using where |
1 row in set (0.01 sec)
The cardinality of status index is woeful, but provided that the application […]
Why InnoDB index cardinality varies strangely
This is a very old draft, from early 2007 in fact. At that time I started to look into something interesting with the index cardinality statistics reported by InnoDB tables. The cardinality varies because it's derived from estimates, and I know a decent amount about that. The interesting thing I wanted to look into was […]
Statistics of InnoDB tables and indexes available in xtrabackup
If you ever wondered how big is that or another index in InnoDB … you had to calculate it yourself by multiplying size of row (which I should add is harder in the case of a VARCHAR – since you need to estimate average length) on count of records. And it still would be quite […]
Google's user_statistics V2 port and changes
Recently Google published V2 release of patches, one of them user_statistics we use in our releases. New features are quite interesting so we decided to port it to fresh releases of MySQL. Features includes: New statistics per user (Cpu_time, Bytes_received, Bytes_sent, etc) New command SHOW CLIENT_STATISTICS, which shows statistics per client's hostname, not per user […]
SHOW INNODB STATUS walk through
Many people asked me to publish a walk through SHOW INNODB STATUS output, showing what you can learn from SHOW INNODB STATUS output and how to use this info to improve MySQL Performance. To start with basics SHOW INNODB STATUS is command which prints out a lot of internal Innodb performance counters, statistics, information about […]
Illustrating Primary Key models in InnoDB and their impact on disk usage
On a recent engagement I worked with a customer who makes extensive use of UUID() values for their Primary Key and stores it as char(36), and their row count on this example table has grown to over 1 billion rows. The table is INSERT-only (no UPDATEs or DELETEs), and the bulk of their retrieval are PK […]


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PlayStation Knowledge Center
If you are able to connect to the Internet but cannot get into Sony Computer Entertainment America's (SCEA) game servers or network, it may be a firewall issue. A firewall is a security product that employs a combination of hardware and software to prevent unauthorized users or Internet traffic from gaining access into a private local area network (LAN) or personal computers. Many routers or modems have a firewall built in to them. In order to allow certain information to pass through, specific TCP and UDP ports on the router or modem will need to be opened in the router or modem's settings.
Below are the TCP and UDP (firewall) port numbers you will need to have open to connect online with PlayStation® products.
- Make sure to enable these TCP/UDP ports in BOTH directions and are entered into the TCP and UDP port fields in your router / modem.
- Contact your Internet Service Provider or router manufacturer for proper instructions on how to input port information for your network.
PlayStation®Network and SCEA Game Servers
All games published by SCEA (first-party) and the PlayStation Network may use the following ports for communication with SCEA (first-party) game servers:
- TCP Ports: 10050, 10051, 10040 - 10060
- UDP Ports: 50000 - 60000
PlayStation Now
- UDP Port: 2053
Other Games
If you need port numbers for games published by third-party companies, contact the publisher of the software title directly. See Game Support Contacts for contact information.
PlayStation®3 Remote Play
PlayStation®3 Remote Play (via the Internet) requires:
- If the router in use supports UPnP, enable the router's UPnP function.
- If the router does not support UPnP, you must set the router's port forwarding to allow communications to the PlayStation 3 from the Internet.
- The port number that is used by remote play is TCP Port: 9293
USB or Bluetooth Headsets
- TCP Port: 80
- UDP Ports: 6000 - 7000, 50000, 10070
Note: headsets must be compatible


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A beautiful wedding ring set for any woman
Your wedding should be a dream come true day. So you want everything to be perfect starting from the jewelry right down to the shoes. And one of the most vital pieces of the ensemble here is the wedding jewelry, which centers on the wedding ring and the band. When you wear a wedding ring set, it always comes with an engagement and a normal band.
It is more convenient to opt for a set because it looks gorgeous. Though it is a personal choice, wedding rings go beyond normal solitaire pieces. For instance, a diamond and blue diamond cluster ring would be perfect with its detailed embellishments and set diamonds. Together, they symbolize the many beautiful memories that the couple would share together.
Check out this platinum over sterling silver diamond cluster ring that enhances the beauty of the bride. It is clustered with 1/10 carat T.W. of precious diamonds. The shimmering ring embodies the best of contemporary luxury lifestyle and is a true treasure! Set in platinum over sterling silver, the ring gives you the lot-a-look for a little!
The diamond band is a fun touch to a wedding ring set because it isn't just a plain band, but it has the beautiful embellishments of a diamonds to add onto the beauty of a diamond ring. A thick diamond band is a symbol of eternal love for the couple. You can personalize it with the name of your spouse or even add a loving message.
Now, you can pair the two rings together, wear them on separate fingers or even individually. It really makes sense to opt for a diamond band because it is a good investment and also doubles as an elegant jewelry piece. You can pick the band in gold, silver or platinum. Also, you can opt for a blend of gold and white for a more contemporary look.


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Advanced Search
Funding, Grants & Awards | News
Netop has launched its 2014 Gap Grant program, putting up $50,000 for blended learning tools.
The $1,000 grants are designed to make the company's Vision and Vision Pro classroom management tools more affordable.
"Vision gives teachers complete control over classroom computers from an easy-to-use console that shows each student computer screen," according to a company news release. "Using Vision, they can monitor student progress, engage students with on-screen demonstrations, control Web browsing and guide student learning."
Features of Vision Pro include cloud-based testing and assessment tools and high-speed screen sharing for graphics-intensive apps.
"Technology is at the very core of today's most innovative — and successful — instructional practices, such as blended learning and flipped classrooms," said Kurt Bager, CEO of Netop, in a prepared statement. "Our 2014 grant program exemplifies our commitment to ensuring that all teachers have access to the tools they need to leverage the power of the devices their students are using to improve achievement."
The deadline for grant applications, available at netop.com, is August 1.
About the Author
Joshua Bolkan is the multimedia editor for Campus Technology and THE Journal. He can be reached at email@example.com.
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Paul VI Catholic School wanted to develop a laptop program that would equip its students and teachers with the right tools to increase classroom learning, prepare their students for college, and to develop the 21st Century skill sets required by today's employers. Download this White Paper to learn about their comprehensive selection process and how they are using their new laptops to revolutionize the learning process.
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This music-filled class celebrates the unique joys of your growing child. Each week your child will love singing, dancing, and playing instruments with you and her new friends and you'll love helping her practice a wide variety of abilities such as gross and fine motor skills, turn-taking, social skills, and active listening. In class, we'll also share tips and ideas for using music throughout the week. Plus, the fun (and learning!) continues with the Kindermusik@Home digital home materials, including music from class, musical activities, and each month's story.
Songs and rhymes. Kid-friendly, age-appropriate songs and chants help develop your toddler's vocabulary and encourage him to find his own voice.
Instrument exploration and play. With you by his side, you and your toddler will explore a variety of instruments, learning about timbre, steady beat, and all the different ways to shake, tap, and jingle along.
Active movement. Experts tell us that movement is key to learning, but movement is also key to keeping an active toddler engaged and focused. Every toddler music class includes creative movement activities designed to help your child develop a strong sense of rhythm, an increased vocabulary, and a joyful outlet for self-expression.
New units each month. Every new unit is an all-new theme filled with delightfully engaging, developmentally appropriate activities for 2 year olds and 3 year olds that inspire a lifelong love of music and lay a strong early foundation for learning.
Kindermusik@Home. Our all-new, easy to use, online learning platform extends the magic and learning of a Kindermusik class into your own home throughout the week. Kindermusik@Home delivers your favorite Kindermusik songs and activities, instrumental music, books, and lyrics—as well as recipes, learning games, crafts, and more in a green-friendly digital format.
Learn how music benefits child development and can help make great parenting easier.
Psychologists, neuroscientists, and experts in early childhood development have demonstrated that music helps children's brain cells make the connections needed for virtually every kind of intelligence. Kindermusik's curriculum is built on this research.
© 2014 Kindermusik International, Inc. All rights reserved.
Interested in Kindermusik classes across the world? Join our international community


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Tracy K.
He is thirty-four and I am twenty-nine. He was divorced and I was a career women. We met on Match.com - our profiles were perfect; it seemed too good to be true. We emailed back and forth, until we swapped phone numbers, and called each other. We talked on the phone for two weeks straight, for three hours every night. I was being relocated to San Diego, where he lived. I thought it was fate.
We met for dinner at this quaint little restaurant for dinner and stayed up all night talking and getting to know each other. He took me for coffee the next morning and drove me to a local lake where we sat and watched the mist rise off the water and the sun shine through the clouds. As we left, we were already planning for a second date. In the middle of the conversation, he stopped me, told me that I was beautiful, and asked to kiss me; I let him.
Five months into our relationship, he asked me to move in. I did, and we were inseparable. I am the luckiest girl alive. One year later, he got down on both knees, as I was sitting at my computer, doing work. He turned me towards him, and said, "If I love you, and you love me, will you spend the rest of your life with me."
He not only had this planned for two months, but snuck into my cell phone to get my parents number to call my dad and ask him if he could marry me. That's about as simple and romantic as we could get. Any other surprising way and I would have known something was up. I am very intuitive, and he knows that. He did it in the comfort of our home.


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In this course,, visual effects guru Mark Christiansen covers the relevant new features of After Effects CS5.5, including the new Warp Stabilizer, 3D Stereoscopic workflow, and Lens Effects tools. The course also covers light falloff enhancements and workflow improvements in this release.
Stabilization in After Effects CS5.5 is totally new. Not only is unlike anything you've seen before built in to After Effects, it's actually more sophisticated than any of the third-party or other custom options you can add to this app. The reason for this is pixel analysis. This tool actually tracks individual pixels throughout the full frame in motion and therefore it can work with different objects moving in separate directions over time and stabilize the whole shot as one.
Having this arrow in your quiver may change the way you shoot and it will raise production values on existing footage you might have previously considered unusable. Let's preview the shot and see what we're starting with. (Bicyclist: Down tubes and seat stays, those are all different in 2010. That's how they got a more compliant?) You can see that although the camera operator clearly did his best to keep this shot steady, he is riding around on the back of a bouncing vehicle, the camera goes slightly Dutch just about a second in, and at about the middle of the clip there's this awkward kind of stairstep-y zoom that occurs as the shot was being reframed.
So this is not a shot that you could just simply lock off and that's what the previous stabilization in After Effects would have offered you. That's why you need Warp Stabilizer. You can apply it in a few different ways. I will use the Animation menu to find the Stabilize Motion command. Now this command existed in previous versions of After Effects but it now does something completely different. As soon as I click it, the Warp Stabilizer effect is applied over here and you see this blue banner telling you that an analysis is already in progress.
You can see the progress over here in the Effect Control panel. It's ripping right through it. I see the percentage and it will show me the time remaining as well and while it's doing it, if it was a longer clip, I'm free to go work elsewhere in After Effects and even open up other comps while that's happening. In this case I'm just going to wait for it. You are going to see it complete and now it's going to the second phase where it actually stabilizes the shot and it's done. Now I'll preview the result, again hitting zero on the numeric keypad to whip up a RAM preview.
(Bicyclist: Down tubes and seat stays, those are all different in 2010. That's how they got a more compliant?) Clearly that's a huge improvement and I didn't have to really do anything at all. If I twirl down the effects in the timeline, you'll see there are in even any keyframes created by this effect. So everything is happening on the first pass automatically.
So you can get started stabilizing any shot in After Effects, as simply as just choosing stabilize motion and then letting the application go to work on the shot.
There are currently no FAQs about After Effects CS5.5 New Features.
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How to Talk to Kids About the Economy
Filed under: Work Life
I would love to, I explained to her. Maybe even next winter if we're careful with our spending. Very careful.
Kids, as a rule, shouldn't hear about their parents' money worries. Those are adult problems, after all. But even young children are feeling the bite of a bad economy when the first things parents cut from the budget is the "fun stuff."
Just because we aren't going to burden our kids with stress and worry doesn't mean we can't teach them a few things about personal finance. And if you're tightening your family's financial belt, now might be the perfect time. Over at wikiHow, they've got an interview with Personal Finance for Dummies author Eric Tyson, where he shares twelve tips for teaching kids about money.
After all, when this economy is finally patched up and set right, it's those same kids we'll be handing it off to. It might be good for them to know how to handle it when we do.
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ReaderComments (Page 1 of 1)
1-29-2009 @ 11:00AMPatti Handy said...
I totally agree that it is important to involve our kids with money issues. Not all issues, of course, but they should be aware of the kind of money it takes to run a household.
As a money coach for kids, I am always encouraging parents to start the conversation. We need to educate and empower our kids today, so they don't have the struggles that many face today.
Patti Handy
1-29-2009 @ 11:53AMSKL said...
It's easier to give than to take away. To me, the most logical approach is to not get them used to "getting" whatever their little hearts desire in the first place. Money is never something to be squandered - even if you're Bill Gates, there are charitable causes to be supported. One unnecessary toy purchase could feed an orphan in a poor country for months, or provide textbooks for a whole school somewhere. There has to be a good reason for each purchase; if you buy stuff just because you "can," it will be harder to get your kids on board when you find you "can't."
1-29-2009 @ 4:34PMame s said...
My 9 & 11 year old daughters receive an allowance. We give them a minimum of $5 a week, up to $15 a week if they do extra jobs. They receive cash from relatives for birthdays & Christmas.
They have understood for years that if they see something in a store & want it, they must pay for it. They are choosy about what they spend money on.
We saved for 2 years for our trip to Disney World. They brought their own spending money. I kept an envelope for each, and once it was spent, that was that.
In December they decided to spend $50 each for a day of horse riding camp. I suggested they should save the money, but they really wanted to go and had a great time.
If they want to go to riding camp for a week this summer, it will be their $200 that pays for it.
1-31-2009 @ 5:44PMSara said...
I couldn't agree with you more. You can do Disney on a budget. We saved for about a year for our trip this past year, and asked the family to give our kids "Disney Dollars." They used their money for what they wanted, and we were relatively off the hook for things we might not have paid for. My 9 year old, after seeing some prices, even gave us "his" money to pay for a meal at the park.
Kids have to be brought up respecting money, and be given the chance to make their decisions. Saving for that trip was probably the best lesson they could have had. They already want to save for another trip in a few years!
As for allowances--I may be mean, but they have to tithe part of their allowance also. But, I am also the mom who makes them get rid of a toy/outfit when they get new things.
3-20-2009 @ 8:18AMChristine Smith said...
My husband lost his job and we do NOT tell the kids. This is his 6th job layoff in ten years and we've learned by our mistakes. Telling the kids Daddy does not have a job kicks off a wave of depression in the entire family. He takes the kids to school in the morning then goes to the job search center and spends all day looking for a new IT job. We simply tell the kids that the economy is really tight, gas prices are affecting our budget and we need to cut our expenses. They accept this and they too want to help.
When my older daughter forgets to turn off her bedroom light, my younger daughter announces it to the entire family "You left your light on again." Now we say "Green Girl, your light is on and you're not saving energy." She thinks its really cool to "Go Green" so we speak her language, help conserve energy, and spend less on utilities.
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EAST STROUDSBURG — Here in Pennsylvania, you have to be 18 or over to buy tobacco products. But in New York City, the legal age has changed to 21.
Shannon Keag is a freshman at East Stroudsburg University. The 19-year-old from Staten Island, New York is also a smoker. She's not happy about a new law in New York City that makes it illegal for her to buy tobacco products there.
"I would like the opportunity, if I choose to go buy them out there, I'm allowed to. Now I'm not," said Keag.
New York City Mayor Michael Bloomberg just signed a new law that makes it illegal for people under 21 to buy cigarettes or other tobacco products in the city.
But Keag believes smokers under 21 will still find a way to get tobacco.
"I've been smoking since I was in eighth grade, like when I was 13. If I could find a way around it then when the age was 18, I'm pretty sure anyone can find a way around it now," said Keag.
Toni Feliciano from the Bronx, New York and is a junior at East Stroudsburg University. She too is a smoker. She feels raising the legal age to buy tobacco is the wrong way to stop people from smoking.
"He should invest in something else. Like maybe a program or something that strays people away from that," said Feliciano.
However, some non-smokers, like Frank Bruno of Lake Wallenpaupack, feel the new law in New York City is a good thing.
"The less people that can buy cigarettes, the less people who will die from lung cancer and diseases like that. So it's smarter," said Bruno, an East Stroudsburg University junior.
When asked if Pennsylvania should adopt a similar piece of legislation to make it illegal for people under the age of 21 to buy tobacco products, you can imagine, most of the people we spoke with say they're not too thrilled about that either.
"No, of course not, no. Come on. How many things are they going to limit to people?" said Feliciano.
"I don't think it would be fair either because my parents aren't around. I'm kind of stuck in another pickle. I have to rely on them to get my cigarettes, which really isn't fair," said Keag.
Right now, the mayor of East Stroudsburg says there is no legislation being discussed to increase the legal age to buy tobacco.
A spokesperson with the Tobacco Free Northeast Foundation says there also isn't any legislation in the state to increase the legal age to buy tobacco.


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Texas Politics
All Texas politics all the time from the San Antonio Express-News and Houston Chronicle
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Lawmaker/doctor endorses hand sanitizer
Rep. John Zerwas, R-Katy, offered some free medical advice from the floor of the House of Representatives this morning regarding the swine flu outbreak: Use hand sanitizer. State photo Rep. John Zerwas, R-Katy He's a physician, so members listened – and House Administration Chairman Charlie Geren, R-River Oaks, announced the Texas Medical Association was delivering […] [Read More]
Health Commissioner: Deaths expected from swine flu in U.S. (UPDATE)
Texas is aggressively targeting swine flu with the expectation that there will be deaths reported from the disease "at some point" in the United States, the state health commissioner said today. Dr. David Lakey, commissioner of the Department of State Health Services, told the House Public Health Committee that only one person with a confirmed […] [Read More]


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AMHERST, Mass. (AP) - In what's become an annual tradition, the University of Massachusetts celebrated the start of the new academic year with a delicious, healthy, record-breaking dish.
About 500 students and staff at the Amherst campus on Monday sliced, diced, pitted and peeled 150 varieties of fruit to create a salad weighing more than 15,000 pounds. The salad was mixed in a 15-foot diameter swimming pool.
It included 20 varieties of apples weighing more than 3,600 pounds; 19 varieties of melon weighing more than 2,500 pounds; peaches; bananas; oranges; berries; and more exotic fruits including quince, passion fruit and rambutan.
A Guinness World Records representative certified the record.
UMass in recent years has started the semester with record-breaking seafood stews and stir fries.
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Pretty Boy Bel Ami
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Alternate Title: Pretty Man / Beautiful Man / Beautiful Boy / Pretty Boy
Summary: Dok Go Ma Te is a macho with an impeccable appearance who has shaken up ten of the top 1% it-girls in South Korea. He is about to share some tips on 'how to get a girl'. Among his women, there is an ordinary girl who falls in love with Ma Te and her name is Kim Bo Tong. The story becomes more interesting when Bo Tong starts to do things to win his heart and the unpredictable romance finally starts.
Genre: Romance, Comedy, Family, K drama, Drama, All,
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Invasion of the Duchy, as Cambridge face Cornish All Blacks and Shelford take on Redruth
Posted: March 14, 2014|
At a time when discussing regionalising National League 2S is high on the agenda, it seems appropriate that both Cornish clubs should be visiting Mid-Anglia tomorrow.
It was supposed to be a blank weekend in the calendar, but the wet weather means that Cambridge will be hosting the Cornish All Blacks and Shelford welcome Redruth to the Davey Field.
Cambridge head into their match with a mounting injury list that could hinder them to the end of the season.
Gareth McComb (shoulder) and Albert Portsmouth (bicep tear) are out of tomorrow's match against the Cornish All Blacks, and the coaching staff are hoping that the injuries are not as bad as initially feared.
James Stokes is also out with a hip flexor problem that is causing a groin injury, and he could be sidelined for six weeks.
Ollie Marriott and Jack Green are back in the team though, and Rob Conquest and Ross Kevan may be on the bench.
Director of rugby Rowland Winter said: "It's Bryn Jenkins first trip back to Cambridge and I'm sure he will want to make an impression and carry on what he did down there (where he was dominant in the line-out in the Cornish All Blacks' win).
"They are a great set piece side and a proper Cornish team like that."
Rory Hutchinson will be part of the Shelford squad for their home game with Redruth, who are a place and two points behind the side from the Davey Field.
And Grant Anderson and Shingai Chiwanga are also set to return to the fray.
But as it was due to be a blank weekend, Warrick Anderson, Sean Mullins, Peter Baird and Redmond Newberry are unavailable while Luke Beales is suspended after receiving three yellow cards for foul play this season.
"We're expecting a really hard game," said Shelford head coach Dick Matthews.
"They have picked up a couple of results recently, including beating the second-placed (Canterbury) team last week.
"Obviously, they are a dominant outfit and have one of the best scrum-halves in the league.
"We're expecting a monumental battle up front."


text:
Chair, Associate Professor
Department of Chemistry
Ph.D., University of Cincinnati
Expertise: Chemistry
Dr. Currie is an associate professor and chair of the Department of Chemistry. She holds a Ph.D. in Analytical Chemistry from the University of Cincinnati. Professor Currie joined the Mount's faculty in 2008. Her research interests include the determination of adulteration in cremain samples using inductively coupled plasma (ICP) and capillary electrophoresis (CE) as well as the investigation of heparin purity using CE.


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- Infowars - http://www.infowars.com -
Germany Threatens Block of EU-Ukraine Trade Deal for Yulia Tymoshenko
Posted By Adan On May 2, 2012 @ 2:47 pm In Global Crisis,Old Infowars Posts Style | Comments Disabled
Tom Parfitt and Bruno Waterfield
The Telegraph
May 2, 2012
Guido Westerwelle, the German foreign minister said on Wednesday that Berlin was prepared to scupper Kiev's "association agreement" with the EU if Mrs Tymoshenko, Ukraine's former prime minister, is not allowed adequate medical care. "We Germans say it cannot be ratified if conditions for the rule of law are not also clearly kept to," he said in a television interview.
The comments came as Austria and Holland announced that government officials and the Dutch royal family would boycott all matches of the Euro 2012 football tournament in Ukraine next month if there was no improvement in Mr's Tymoshenko's conditions. Austrian foreign minister Michael Spindelegger said it was "our sign of solidarity" with the politician.
European Commission head José Manuel Barroso has already refused to attend the tournament, and six European presidents will not travel to a summit in Ukraine next week.
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Methods are disclosed wherein labeled antibodies can be provided in vivo to tissue having antigens that specifically bind the labeled antibody. A first optical radiation is emitted into the tissue in vivo to excite the labeled antibody bound to the antigen in vivo. A second optical radiation that is emitted by the excited labeled antibody, in response to the excitation thereof, can be detected in vivo. Related telemetric circuits and compositions of matter are also disclosed.
1. A circuit for detecting biomolecules in vivo, the circuit comprising
an optical radiation source configured for in vivo use that emits first optical radiation;
an optical radiation detector configured for in vivo use that detects second optical radiation emitted by excited labeled binding molecules; and
a processor circuit, coupled to the optical radiation source and the optical radiation detector,
wherein the processor circuit is configured to release fluorescently labeled antibodies selected to bind with predetermined Tumor Specific Antigens (TSAs),
the processor circuit is further configured to activate the in vivo optical radiation source after a predetermined first time interval after release of the fluorescently labeled antibodies, the predetermined first time interval selected to allow a first portion of the fluorescently labeled antibodies to bind with local available TSAs and a second portion of the fluorescently labeled antibodies to become remote from the circuit so that the first optical radiation excites the first portion of the fluorescently labeled antibodies bound with the local available TSAs and does not excite the second portion of the fluorescently labeled antibodies that become remote,
the processor circuit is further configured to sense a voltage generated by the in vivo optical radiation detector after a second predetermined time interval, the second predetermined time interval being after emission of the first optical radiation has ceased.
2. A circuit according to
3. A circuit according to
4. A circuit according to
5. A circuit according to
6. A circuit according to
an emission filter coupled to the optical radiation source; and
an absorption filter coupled to the optical radiation detector.
7. A circuit according to
an inductor coupled to the processor, wherein the inductor provides power to the circuit in response to a power signal received from the ex vivo system.
8. A circuit according to
9. A circuit according to
10. A circuit according to
11. A circuit according to
12. A circuit according to
13. A circuit according to
14. A circuit according to
a piezoelectric circuit responsive to the processor circuit, wherein the piezoelectric circuit is configured to vibrate under control of the processor circuit to release the labeled binding molecules.
15. A circuit according to
16. A circuit according to
The present application claims priority to U.S. Provisional Application No. 60/247,574 filed Nov. 9, 2000, entitled Methods, Circuits, and Compositions of Matter for In Vivo Detection of Biomolecule Concentrations Using Fluorescent Tags, the entire disclosure of which is hereby incorporated herein by reference.
The present invention relates to the field of sensors, and more particularly, to biomolecular sensors.
The ex vitro study of malignant cell populations has established some general principles by which clinical treatment protocols are developed. These principles have established differences between malignant and normal cell populations and have been employed in the treatment of malignant disease. There have been attempts to exploit these differences, both in pre-clinical and clinical studies, to obtain total tumor cell kills and improved cure rates.
One of the major obstacles in achieving this goal has been the difficulty in minimizing normal tissue toxicity while increasing tumor cell kill (therapeutic index). Thus, some treatment strategies employ an empirical approach in the treatment of malignant disease. In particular, the time of delivery and dose of cytotoxic agents can be guided more by the response and toxicity to normal tissue than by the effects on the malignant cell population.
Unfortunately, this approach may not provide accurate information on the changes during treatment of a malignant cell population. Making this information available may allow clinicians to exploit the differences between malignant and normal cells, and hence improve the treatment procedures.
There have been a number of attempts to study changes that occur within a cell population. However, these attempts have not shown the ability to monitor the changes on a real time basis. Indeed, these methods typically provide information at one point in time and most are designed to provide information on one particular function or parameter. In addition, most of the conventional methods can be expensive as well as time consuming. This can be problematic for patients undergoing extended treatment periods typical of radiation and chemotherapy, especially when it is desirable to follow changes both during an active treatment and subsequent to the active treatment.
In addition, tumors may have periods in which they are more susceptible to treatment by radiation or drug therapy. Providing a monitoring system which can continuously or semi-continuously monitor and potentially identify such a susceptible condition could provide increases in tumor destruction rates.
Numerous tumor specific antigens (TSA) have been identified and antibodies specific for a number of these TSA's are known. For example, it has been demonstrated that sigma-2 receptors found on the surface of cells of the 9L rat brain tumor cell line, the mouse mammary adenocarcinoma lines 66 (diploid) and 67 (aneuploid), and the MCF-7 human breast tumor cell line may be markers of tumor cell proliferation. See Mach R H et al., Sigma 2 receptors as potential biomarkers of proliferation in breast cancer. Cancer Res 1997 Jan. 1; 57(1):156-61; Al-Nabulsi I et al., Effect of ploidy, recruitment, environmental factors, and tamoxifen treatment on the expression of sigma-2 receptors in proliferating and quiescent tumour cells. Br J Cancer 1999 November; 81(6):925-33. Such markers may be amenable to detection by non-invasive imaging procedures. Accordingly, ligands that selectively bind sigma-2 receptors may be used to assess the proliferative status of tumors, although in vivo techniques utilizing such ligands have heretofore not been known. Although the field of tumor-specific treatment is still relatively unsettled, various researchers have proposed several potentially important techniques useful in such treatment. For example, the ex vitro detection of biomolecules can be useful in predicting the timing for advantageous treatment of tumors. Many of these techniques use a "hybridization event" to alter the physical or chemical properties associated with the biomolecules. The biomolecules having the altered property can be detected, for example, by optical or chemical means.
One known technique for the detection of biomolecules, called Enzyme-Linked Immunosorbent Assay (ELISA), involves the detection of binding between a biomolecule and an enzyme-labeled antibody specific for the biomolecule. Other methods of detecting biomolecules utilize immunofluorescence, involving the use of a fluorescently labeled antibody to indicate the presence of the biomolecule. The in vivo use of these techniques may involve an invasive introduction of a sensor into the in vivo site to be analyzed. Moreover, these techniques may not be reliable if the surface where the sensor and the tissue interact is not clean. In particular, in vivo use can cause a sensor to become "bio-fouled" over time such that the operational properties of the sensor may change. In particular, proteins may begin to develop on the sensor within minutes of insertion of the sensor into the tissue, which may cause the sensor to operate improperly. In view of the foregoing, there remains a need for circuits, compositions of matter, and methods which can be used to, inter alia, detect biomolecular concentrations in vivo.
Methods according to embodiments of the present invention can include providing labeled antibodies in vivo to tissue having antigens that specifically bind the labeled antibody. A first optical radiation is emitted into the tissue in vivo to provide excite the labeled antibody bound to the antigen in vivo. A second optical radiation that is emitted by the excited labeled antibody in response to the excitation thereof can be detected in vivo. In some embodiments, the labeled antibodies are fluorescently labeled antibodies.
In other embodiments, the step of providing can include releasing the labeled antibodies in vivo from a matrix material over time. In other embodiments, the step of providing can include releasing the labeled antibodies in vivo from a matrix material responsive to a control circuit located in vivo.
In some embodiments, the step of exciting can include emitting the first optical radiation through a bio-fouling tissue. In other embodiments, the step of detecting can include detecting the second optical radiation through a bio-fouling tissue.
Accordingly, labeled antibodies can bind antigens associated with tumor cells. A radiation source can be used to excite the labeled antibodies bound to the antigens. The labeled antibodies emit a second optical radiation in response to the excitation. A sensor can be used to detect a level of the optical radiation emitted by the labeled antibodies. The level of the second optical radiation can be used to determine the concentration of antigens present. The growth or proliferation of the tumor cells may be approximated from the concentration of antigen. Embodiments of the invention advantageously integrate the ability to probe fluorescently tagged entities with an implantable sensor platform, thus allowing accurate, real time determinations of antigen concentration in vivo.
The present invention now will be described more fully hereinafter with reference to the accompanying drawings, in which preferred embodiments of the invention are shown. This invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein; rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the invention to those skilled in the art. Like numbers refer to like elements throughout. In the figures, certain layers, regions, or components may be exaggerated or enlarged for clarity.
The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used in the description of the invention and the appended claims, the singular forms "a", "an" and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise.
Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. All publications, patent applications, patents, and other references mentioned herein are incorporated by reference in their entirety. The term "tissue," as used herein, can include cells, organs, bodily fluids, and other biological matter in a biological sample or the body of a subject. For example, the term tissue can be used to describe cells, organs and/or other biological matter in a human body. The term "biomolecule" can include tumor specific antigens (TSA), such as proteins associated with particular types of tumor cells. It will be understood that the present invention may be used for in vivo use or for ex vitro use. It will also be understood that the term "in vivo" is specifically intended to encompass in situ applications.
In a preferred embodiment of the present invention, biomolecules (e.g., antigens) associated with hyperproliferative cells (including tumors, cancers, and neoplastic tissue, along with pre-malignant and non-neoplastic or non-malignant hyperproliferative cells) are detected. The term "tumor" is generally understood in the art to mean an abnormal mass of undifferentiated cells within a multicellular organism. Tumors can be malignant or benign. Preferably, embodiments of the inventions disclosed herein are used to detect biomolecules associated with malignant tumors. Examples of tumors, cancers, and neoplastic tissue associated with the biomolecules that can be detected by embodiments of the present invention include but are not limited to malignant tumors such as breast cancers; osteosarcomas; angiosarcomas; fibrosarcomas and other sarcomas; sinus tumors; ovarian, uretal, bladder, prostate and other genitourinary cancers; colon esophageal and stomach cancers and other gastrointestinal cancers; lung cancers; myelomas; pancreatic cancers; liver cancers; kidney cancers; endocrine cancers; skin cancers; and brain or central and peripheral nervous (CNS) system tumors, malignant or benign, including gliomas and neuroblastomas. Biomolecules associated with premalignant and non-neoplastic or non-malignant hyperproliferative tissue include but are not limited to biomolecules associated with myelodysplastic disorders; cervical carcinoma-in-situ; familial intestinal polyposes such as Gardner syndrome; oral leukoplakias; histiocytoses; keloids; hemangiomas; psoriasis; and cells made hyperproliferative by viral infections (e.g., warts).
Although the present invention is described herein with reference to the detection of antigens associated with tumor and other hyperproliferative cells, the present invention may also be utilized for the measurement of glucose, cell necrosis byproducts, cell signaling proteins, and the like.
The embodiments of the present invention are primarily concerned with use in human subjects, but the embodiments of the invention may also be used with animal subjects, particularly mammalian subjects such as primates, mice, rats, dogs, cats, livestock and horses for veterinary purposes, and for drug screening and drug development purposes.
As used herein, the term "optical radiation" can include radiation that can be used to transmit signals in tissue, such as radiation in the visible, ultraviolet, infrared and/or other portions of the electromagnetic radiation spectrum.
Although the embodiments described herein refer to fluorescently labeled binding molecules (i.e., antibodies), it will be understood that the present invention may be used with any type label, including fluorescent labels (e.g., fluorescein, rhodamine), radioactive labels (e.g., 35S, 125I, 131I), bioluminescent labels (e.g., biotin-streptavidin, green fluorescent protein (GFP)), and enzyme labels (e.g., horseradish peroxidase, alkaline phosphatase).
It will also be understood that while embodiments described herein refer specifically to antibodies, the present invention may also be used with other molecules that bind the biomolecules to be detected. Furthermore, although the present invention is described with reference to detecting concentrations of antigens, the present invention may also be used to detect the concentration of any biomolecules whose detection is desired, including but not limited to proteins, polypeptides, nucleic acids, polysaccharides, and the like.
As used herein, the term "antibody" is understood to encompass all antibodies as that term is understood in the art, including but not limited to polyclonal, monoclonal, chimeric, and single chain antibodies, Fab fragments, and fragments produced by a Fab expression library. Monoclonal antibodies may be prepared using any technique which provides for the production of antibody molecules by continuous cell lines in culture. These include, but are not limited to, the hybridoma technique, the human B-cell hybridoma technique, and the EBV-hybridoma technique. See, e.g., G. Kohler et al. (1975) Nature 256, 495-497; D. Kozbor et al. (1985) J. Immunol. Methods 81, 31-42; R. J. Cote et al. (1983) Proc. Natl. Acad. Sci. USA 80, 2026-2030; and S. P. Cole et al.(1984) Mol. Cell Biol. 62,109-120.
Chimeric antibodies may be produced according to methods set forth in, for example, S. L. Morrison et al. (1984) Proc. Natl. Acad. Sci. 81, 6851-6855; M. S. Neuberger et al. (1984) Nature 312, 604-608; and S. Takeda et al. (1985) Nature 314, 452-454). Alternatively, techniques described for the production of single chain antibodies may be adapted, using methods known in the art, to produce antigen-specific single chain antibodies. Antibodies may also be produced by inducing in vivo production in the lymphocyte population or by screening immunoglobulin libraries or panels of highly specific binding reagents as disclosed in the literature. See, e.g., R. Orlandi et al. (1989) Proc. Natl. Acad. Sci, 86, 3833-3837; and G. Winter et al. (1991) Nature 349,293-299. Antibodies with related specificity, but of distinct idiotypic composition, may be generated by chain shuffling from random combinatorial immunoglobulin libraries. See e.g., D. R. Burton (1991) Proc. Natl. Acad. Sci. 88,11120-11123).
Antibody fragments which contain specific binding sites for antigens can also be used. For example, such fragments include, but are not limited to, the F(ab′)2 fragments which can be produced by pepsin digestion of the antibody molecule and the Fab fragments which can be generated by reducing the disulfide bridges of the F(ab′)2 fragments. Alternatively, Fab expression libraries may be constructed to allow rapid and easy identification of monoclonal Fab fragments with the desired specificity. See W. D. Huse et al. (1989) Science 254,1275-1281.
Fluorescence-based assays are well established for ex vitro studies and a number of fluorophores and tagged antibody systems are commercially available. An extensive list of commercially available pH-dependent fluorophores useful in the practice of the present invention can be found in R. P. Haugland, Chapter 23 ("pH Indicators") of Handbook of Fluorescent Probes and Research Chemicals, Sixth Edition (Molecular Probes, Inc. Eugene, Oreg., (1996), and HTML version located at www.probes.com).
According to embodiments of the present invention, fluorescently labeled binding molecules, such as antibodies, can be bound to biomolecules, such as antigens, associated with tumor cells. An optical radiation source can be used to excite the fluorescently labeled antibodies bound to the antigens. The fluorescently labeled antibodies emit a second optical radiation in response to the excitation. A sensor can be used to detect a level of the optical radiation emitted by the fluorescently labeled antibodies. The level of emitted optical radiation can be used to determine the concentration of antigens present. The concentration of antigen may then be correlated to the amount, or the presence, or the growth or proliferation behavior of the tumor cells based on known relationships between concentration of tumor specific antigen and these parameters, or according to relationships that may be determined by the skilled artisan.
The phase of the tumor tissue 110 may be detected based on a concentration level of the TSA 195 at the surface 100. For example, a "growth" phase of the tumor may be characterized by relatively high concentrations of the TSA 195 and a "remission" phase may be characterized by relatively low concentrations of TSA 195.
A platform 105 is located in vivo proximate to the tumor tissue 110 and may or may not become bio-fouled with a bio-fouling tissue 190 over time. The platform 105 carries a matrix material 140 that can include fluorescently labeled antibodies 130 that are suspended in the matrix material 140. The matrix material 140 can be soluble so that the fluorescently labeled antibodies 130 can be released from the matrix material 140 over time. The matrix material 140 can be in the shape of a cylinder as shown, for example, in
The fluorescently labeled antibodies 130 are selected to specifically interact or bind with the TSA 195 that characterizes the tumor tissue 110, but is not associated with normal tissue. More than one TSA 195 may characterize a the tumor tissue 110. When the fluorescently labeled antibodies 130 are released from the matrix material 140, some of the fluorescently labeled antibodies 130 bind with the TSA 195 on the surface 100 proximate to the platform 105 to form a binding complex 160. The unbound fluorescently labeled antibodies 150 may dissipate over time to become remote from the platform 105.
An optical radiation source 120 emits a first optical radiation 170 that excites the fluorescent labels of the binding complexes 160 to a higher energy state. In one embodiment of the invention, the first optical radiation is emitted through a biofouling tissue 190. Once excited, the fluorescent labels of the bound complexes emit a second optical radiation 180. The respective wavelengths of the first optical radiation 170 and the second optical 180 may be selected to promote penetration of the bio-fouling tissue 190. The optical radiation source can be, for example, a laser diode, a high power Light Emitting Diode (LED), or the like, as described further herein.
An optical radiation detector 115 can detect the second optical radiation 180 through bio-fouling tissue 190 thereby avoiding some of the drawbacks associated with conventional techniques. A time interval between the emission of the first optical radiation 170 and detection of the second optical radiation 180 can be selected to allow the fluorescently labeled antibodies 130 to bind with the TSA 195 on the surface 100. The optical radiation detector 115 can be a photodiode or a phototransistor. Other devices as described further herein and/or known to those skilled in the art and may be also be used.
The optical radiation detector 115 can include an optical absorption filter to reduce the effects of background noise. The optical radiation source 120 and the optical radiation detector 115 can be separated by a shield that reduces the amount of the first optical radiation 170 that reaches the optical radiation detector 115. In some embodiments, the optical radiation detector 115 is located about 500 micrometers from the bound complexes 160. In other embodiments, the optical radiation detector 115 includes a lens that collects and focuses the second optical radiation 180 so that the separation between the optical radiation detector 115 and the bound complexes 160 may be increased.
The intensity of the second optical radiation 180 can be used to determine the concentration of the TSA 195. In particular, the TSA 195 that is proximate to the platform 105 may have fluorescently labeled antibodies 130 bound thereto. Accordingly, the fluorescent labels may emit the second optical radiation 180 after the excitation of the first optical radiation 170.
The unlabeled and fluorescently labeled antibodies 220, 210 can be released continuously over time or in phases as described herein. The release of the respective antibodies may be out of phase with respect to each other. For example, unlabeled antibodies 220 may be released during a first time interval and the fluorescently labeled antibodies 210 may be released during a second time interval. The antibodies may also be released using an apparatus 270 coupled to the respective matrix material, as described further herein. The apparatus 270 coupled to each matrix material may be different. In some embodiments, the apparatus 270 may be used to control the rate of release of the unlabeled and/or labeled antibodies. The use of a controlled release strategy can be employed to provide a continuous source of fluorescently-labeled antibody 230, which can be advantageous in the dynamic biological environment in which the platform 200 must function.
The unlabeled antibodies 220 are released into the tissue 290 to provide free unlabeled antibodies 235. The fluorescently labeled antibodies 210 are released to provide free fluorescently labeled antibodies 230. Some of the free fluorescently labeled antibodies 230 bind to the antigens 205 to provide bound antigens 231. Some of the bound antigens 231 become bound to the unlabeled antibodies 220 at the surface of the second matrix material 240 to provide bound structures 290 at the surface of the second matrix material 240. An optical radiation emitter/detector 285 is adjacent to the second matrix material 285 and can be used to excite the bound structures 290 and detect a signal as discussed above.
It will be understood that other fluorescently labeled antibodies may be included in the matrix material 335 to provide a mixture of different types of antibodies. The term "different types of antibodies" will be understood to meant that one type of antibody may have more than kind of label, i.e., label A and label B. Alternatively, more than one type of antibody (i.e., antibody A and antibody B) may have the same label. For example, the matrix material 335 can include type A and type B fluorescently labeled antibodies 330. Moreover, the A and B type fluorescently labeled antibodies 330 may have different concentrations. For example, the A type fluorescently labeled antibodies 330 can comprise 20% of the fluorescently labeled antibodies 330 and the type B fluorescently labeled antibodies 330 can comprise 80% of the fluorescently labeled antibodies 330. Additional types of fluorescently labeled antibodies 330 may also be included in varying concentrations.
It is preferable that the matrix material 335 not react with or damage the fluorescently labeled antibodies 330 suspended therein. It is also preferable that the matrix material 335 not promote bio-fouling at the interaction surface 340 so that the fluorescently labeled antibodies 330 may be released over time without undue interference. The matrix material 335 may comprise one or more of several polymers. The choice of polymer can be determined empirically as encapsulation, degradation and release characteristics of polymers in tissue may vary from subject to subject, or from cell type to cell type, or from sample to sample, and the like. Suitable biodegradable polymers can be based on hydrolysis of ester linkages in the polymer, and a variety of polymers of this type are commercially available and well characterized. Many of these polymers degrade into small, non-toxic molecules. Some of the most common biodegradable polymers are poly(lactic acid) and poly(glycolic acid). Fried, Joel R. Polymer Science and Technology, Englewood Cliffs, N.J., Prentice Hall, 1995, pp. 246-249. In some embodiments according to the present invention, the matrix material 335 is a mixture of different materials such as a combination of polylactic acid and polyglycolic acid. The different materials can occur in a range of concentrations. For example, the matrix material 335 can comprise between about 0 and about 50% polylactic acid and/or between about 10 and about 50% polyglycolic acid.
In some embodiments, time release of the fluorescently labeled antibodies 330 may be controlled by selecting the matrix material 335 based on the biocompatibility of the material 335 with the antibody or biomolecule to be detected, polymer type, polymer structure (e.g., the physical size and porosity of the polymer release bead), the molecular weight of the matrix material 335, the porosity of the matrix material 335, and/or other material parameters.
In other embodiments, the matrix material 335 may be coupled to an apparatus 350 that can affect the rate at which the matrix material 335 releases the fluorescently labeled antibodies 330. For example, the apparatus 350 can be a piezoelectric circuit that vibrates the matrix material 335, thereby causing the fluorescently labeled antibodies 330 to be released at varying rates. Although several parameters (e.g., polymer structure, molecular weight, porosity, etc.) are available to control the rate and time course of release, other techniques for controlling release may be used. For example, the polymer may be mounted on top of a piezoelectric element, whereby the actuation of the element (e.g., mechanically shaking the polymer with a sinusoidal input to the piezoelectric) increases the rate of release. Another option for modulating release rate is to blend the matrix material 335 with an electrically conducting polymer (e.g., polypyrrole) and, by oxidizing and reducing the polymer electrochemically, modulate the porosity of the blend (Kontturi et al., "Polypyrrole as a model membrane for drug delivery", Journal of Electroanalytical Chemistry, 1998, 453(1-2), 231-238, Hepel, M. et al., "Application of the electrochemical quartz crystal microbalance for electrochemically controlled binding and release of chlorpromazine from conductive polymer matrix", Microchemical Journal, 1997, 56, 54-64, Yano, S. et al., "Extracellular release of a recombinant gene product by osmotic shock from immobilized microalga in electroconductive membrane" Bioelectrochemistry and Bioenergetics, 1996, 39, 89-93, Bidan et al., "Incorporation of Sulfonated Cyclodextrins into Polypyrrole—An Approach for the Electro-controlled delivering of Neutral-Drugs", Biosensors & Bioelectronics, 1995, 10, 219-229, Hepel, M. et al., "Electrorelease of Drugs from Composite Polymer-Films" ACS Symposium Series, 1994, 545, 79-97.
An optical radiation source 505 can include an amplifier that responds to a control input A to provide an output current that passes through a high power light emitting diode that emits optical radiation 515. The optical radiation 515 can pass through a bio-fouling tissue 570 and excite the fluorescent labels on the fluorescently labeled antibodies.
The excited fluorescent labels can emit an optical radiation 520 that can pass through the bio-fouling tissue 570 to reach an optical radiation detector 510. For example, the optical radiation 520 impinges a photodetector. In response, the photodetector can generate a current that can be converted to a voltage level that represents the level of the optical radiation 520. In some embodiments according to the present invention, the photodetector is a photomultiplier. The optical radiation detector 510 can include an absorption filter to reduce background noise.
The optical radiation source 505, the optical radiation detector 510, and the matrix material 530 can operate in conjunction with a processor circuit 525. The processor circuit 525 can control the release of the fluorescently labeled antibodies from the matrix material 530 by controlling the apparatus 580 that, for example, vibrates the matrix material 530 to vary the rate of release of the fluorescently labeled antibodies.
The processor circuit 525 can provide an input to the optical radiation source 505. The processor circuit 525 can monitor an output signal C from the optical radiation source 505 to determine, for example, the power output thereof. Other functions may be monitored and/or controlled.
The processor circuit 525 can receive a voltage level B from the optical radiation detector 510 to determine, for example, the intensity of the optical radiation 520. The processor can provide an output E to a telemetry system (526). The telemetry system 526 can transmit/receive data to/from an ex vitro system (not shown). The ex vitro system can control the release of the fluorescently labeled antibodies by transmitting a signal into the body for reception by the in vivo system. The in vivo system can release fluorescently labeled antibodies in response to the signal from the ex vitro system. Other signals can be transmitted from the ex vitro system. In some embodiments, the transmitted/received data is digitally encoded. Other types of data transmission may be used.
The in vivo system can transmit data to the ex vitro system. For example, the in vivo system can transmit data associated with the intensity of the optical radiation 520. The in vivo system can transmit other data to the ex vitro system. Accordingly, the in vivo system can be implanted for in vivo use whereby the ex vitro system can control operations of the in vivo system including receiving data from the in vivo system without an associated invasive procedure.
In some embodiments, the in vivo system is powered remotely through the tissue in which it is implanted. For example, the in vivo system can include an inductor that provides power to the in vivo system via an inductively coupled power signal from the ex vitro system. In some embodiments, the in vivo system has a diameter of approximately 2 mm.
In the embodiments of the invention described above, a light emitting diode (LED) or laser diode (for greater excitation intensity) can be used as the excitation source and a photodiode can be used to detect the corresponding emission signal. Integral emission and absorption filters can be introduced as needed in the form of dielectric coatings on the diode elements. Light emitting diodes, and photodetectors are now commonly available. These devices can be extremely compact, with a laser diode being typically less than 100 μm. Thin film deposition and fiber optic technologies known to the skilled artisan permit the construction of extremely sharp optical filters.
An external sensor package for the optical implant apparatus described above may be about 2 mm�10 mm in the form of a rounded cylinder. This configuration may ease insertion into a subject when used in conjunction with a device similar to a biopsy needle. The standardization of package size and geometry may enable a diverse range of coatings such as diamond like carbon (DLC) or glasses of various compositions and plastics. The inner portion of the package can be used to provide a hermetic seal isolating the device from the effects of moisture and attack by the body.
In some embodiments, laser diodes are mounted on a heat sink and emit light from front and rear facets perpendicular to the circuit board. The optical power from the rear facet can be measured by a photodetector mounted on the opposite side of the circuit board. This permits feed back control of the optical power. On one side of the optical barrier dividing the cylinder, a signal photodiode receives the return fluorescence or the absorption signal to be ratioed, as in the case of oxygen measurements. An optical rejection filter can be deposited on the photodetector to reduce background noise. The telemetry coil, drivers and other electronics can be distributed on either side of the circuit board.
The embodiments of the invention described herein may afford effective baseline correction, a potentially important consideration in the practice of the present invention. Changes in diode laser output as a function of time can be accommodated through the use of standard photodiode feedback techniques. Measurements before and after insertion can be used to provide an initial baseline. This may be helpful in assessing background fluorescence and the degree of non-specific binding. The influence of external lighting as a parameter may also be assessed. The lifetime of the implant may be as long as six months or even more in some cases.
One advantage of this detection scheme is that it may be relatively resistant to the accretion of material on the outer surface of the sensor ("biofouling"). One aspect of the invention provides for emission and absorption wavelengths through whatever over layer covers the sensor surface. The circuit may also be coated with a biocompatible optical translucent layer. Although close proximity of the target fluorophore to the sensor is desirable, significant leeway is obtained for detection of signals away from the site of sensor implantation. As discussed herein, one embodiment includes a time-released, tagged antibody or event-activated hybridization reaction. Continuous monitoring of the implanted sensor is possible so that kinetics of the reaction can also be assessed.
In embodiments of the present invention, a lens system may or may not be present, but the detector is preferably placed in close proximity (e.g., about 500 micrometers) to the source of fluorescence. In this way, the detector may become the image plane. The sensor may alternatively be non-imaging and accordingly may be used as a binary-state detector for the presence or absence of fluorescent signal.
As disclosed above, according to embodiments of the present invention, fluorescently labeled antibodies can be coupled to antigens associated with tumor cells. An optical radiation source can be used to excite the fluorescently labeled antibodies coupled to the antigens. The fluorescently labeled antibodies emit optical radiation in response to the excitation. A sensor can be used to detect a level of the optical radiation emitted by the fluorescently labeled antibodies. The level of optical radiation can be used to determine the concentration of antigens present on the surface of the tissue. The concentration of antigens may then be correlated to the proliferative state or growth behavior of the tissue. In the drawings and specification, typical preferred embodiments and methods according to the present invention have been disclosed. Although specific terms have been used, they are used in a generic and descriptive sense only and not for purposes of limitation, the scope of the present invention being set forth in the following claims.
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What is the most boring part of being a DBA? Many of us reply with "installing and creating a database". Really, that is not true, at least not creating a database. If you think create database is a boring ad-hoc work and takes a whole day to do, that is because you used wrong tool or you misunderstood what is going on.
DBCA is what you need to create a test database in only 5 mins.
You don't need X windows
You don't need VNC
All you need is DBCA
The next time you are in the following familiar scenario:
Boss: I want a database to test the new application, do it ASAP!
You can reply 5 minutes later:
DBA: Boss, here's your database!
Sounds cool? Here it is:
dbca -Silent -CreateDatabase -gdbName $you_oracle_db_name -templateName $template_name [-characterSet] [-nationalCharacterSet] [-datafileDestination] [-databaseType] ....
dbca -h
will show you how to explore the power of DBCA!
Example: 5 minute database creation
C:\>%oracle_home%\bin\dbca -Silent -createDatabase -gdbName myoracle -templateName %oracle_home%\assistants\dbca\templates\General_Purpose.dbc -characterSet WE8MSWIN1252 -nationalCharacterSet ALU32UTF16 -datafileDestination c:\temp -databaseType MULTIPURPOSE
Copying database files
1% complete
3% complete
11% complete
18% complete
26% complete
37% complete
Creating and starting Oracle instance
40% complete
45% complete
50% complete
55% complete
56% complete
57% complete
60% complete
62% complete
Completing Database Creation
66% complete
70% complete
73% complete
85% complete
96% complete
100% complete
Look at the log file "c:\oracle\product\1020\cfgtoollogs\dbca\myoracle\myoracle0.log" for further details.
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4 Responses to "Creating a test database in 5 minutes"
thank you. that's fun…;)
Awsome… thanks !
Have you tried to create your own seed db along with the customzied template? Yes, we can always use rman to clone a db, but just feel using dbca has more fun.
For more silent install fun, i urge everyone to look at Gregory Guillou's series on RAC install, de-install, and everything in between using DBCA almost exclusively.
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With all teams in action last night we made a reliable set of predictionw again. 8 of our 10 predictions went our way!! 80% is a good grade. We also nailed the London score bang on (4-1). Today's pictured player is Defenceman Brandon Prophet of the Saginaw Spirit. He opened the scoring and then notched another goal in the second period as his team went on to embarrass the host Ottawa 67's by a score of 8-3. Brandon is from Brockville (Eastern Ontario) and will be playing at Belleville tonight. He has 4 points in his OHL rookie season.
Oshawa at Erie
The Oshawa Generals had to board the bus and make the trip to Erie after they hosted the Barrie Colts last night. The Colts beat them despite a pair of goals from Boone Jenner - his 16th and 17th of the season. Tyler Biggs had a big night as well setting up both of Jenner's goals, and one by Oshawa's first round pick Michael Dal Colle(7th of the season).
Dal Colle has been very impressive for the Generals of late – scoring his 2 goal and 2 assist in their game against the Kingston Frontenacs in Oshawa last Sunday evening. Boone Jenner continues to lead the team. Tyler Biggs had a goal and 3 assists in that game as well. Undrafted German born forward, Sebastian Uvira has turned his play up of late and is one of the hardest working players on the team.
The Otters are led by two Subway Super Series performers in Connor McDavid, and Connor Brown. Brown had 2 goals and 2 assists in last night's 6-3, home ice win over the Peterborough Petes – he also leads the league with 11 powerplay goals. McDavid had 2 goals and 1 assist, as the Petes had no answer to the Otters' best players. Goalie Oscar Dansk stopped 41 of 44 shots to earn his 4th win of the season.
My prediction Oshawa 6 Erie 3
Peterborough at Niagara
The Petes were beaten in Erie last night 6-3. Slater Koekkoek picked up a couple of assists in a losing cause for Peterborough. Andrew D'Agostini was lifted at 2:59 of the 2nd period after giving up the 3rd Erie goal. Michael Giugovaz played the rest of the game.
Niagara gave up two powerplay goals to the Steelheads last night, in Mississauga, en route to a 6-3 loss. 4th year winger, Mitchell Theoret picked up his 7th and 8th goals of the season, and Brett Ritchie, his 15th for Niagara. The Icedogs continue to search for secondary scoring – will it arrive tonight? On a penalty kill in the second period, Ryan Strome was assessed a 5 minute major and a game misconduct for a check from behind. The Icedogs were also called for a too many men on the ice penalty late in the game – after the play was stopped it was drawn to his attention that goalie Festerani was on the bench (whoops).
My prediction Peterborough 2 at Niagara 4
Saginaw at Belleville
The Saginaw Spirit started their eastern swing on a positive note last night, as they defeated the Ottawa 67s at Scotiabank Place 8-3. Rookie defenseman Brandon Prophet - Saginaw's second round pick in the 2012 Priority Selection - had his biggest night in his OHL career. Even former 67 Ryan Perklin scored for Saginaw (3rd goal in his 67th OHL game). Jake Patterson was solid in goal for the Spirit. It stands to reason that back-up goalie, Russian import, Nikita Serebryakov will get one of the starts this weekend, as the Spirit will play 3 in 3. His performance has been less than stellar, as he has a GAA average of 4.97 and a Save % of 0.865 in 3 losses.
It'll be interesting to see how the Bulls respond after losing to the Kingston Frontenacs 2-0 last night, especially since Kingston's winning goal was scored with less than 5 minutes left in regulation time. For the second game in a row, the home team has earned a shutout in this series. The Bulls had the better of the chances in last night's game, but couldn't find a way to beat Mike Morrison in Kingston's net. Malcolm Subban earned the game's second star, with some great saves, especially in the first and third periods of the game. Daniil Zharkov returned late in the 3rd after being high sticked in the face. He along with acting captain Joseph Cramarossa were Belleville's best forwards despite being on the ice for both Kingston goals. Jordan Subban skated miles on the Bulls defense.
My prediction Saginaw 2 at Belleville 3
London at Plymouth
Last night the Knights were able to smother the visiting Sudbury Wolves in London Ontario. The Knights jumped out to an early 2-0 lead in the first period via goals by Griffith (tied for fifth in the OHL) and Jammes' first career OHL goal. The Knights would score twice more (M Rupert & Pawley's first) before Sudbury's Brody Silk would damage Jake Paterson's hopes for his second career shutout. The Knights are comfortably in fifth place of the Western Conference within 2 points of third place. Their record now stands at 11-5-0-2.
The Whalers were able to deal with the Spits last night to the tune of 5-3. They scored four straight goals in a 12 minute span to end the first period and start the second period. Their powerplay was 3/9 and the Windsor powerplay was 3/10 in a penalty filled contest. Stefan Noesen continues to shine this month with 5 goals and 3 assists in 4 games played. Garreett Meurs also had a good game with one goal and 2 assists after being held off the scoresheet for the past 2 games.
My prediction London 4 Plymouth 3 (OT)
Brampton at Barrie
The Battalion hosted the #1 Owen Sound Attack last night in a back and forth affair. The game was tied 3-3 until late in third period – then an empty net goal gave the final score of 5-3 in favor of the Attack. Bell, McLeod and Childerley were the goal scorers. Barclay Goodrow is in a mini-slump as he has only 2 goals and 0 assists in the past 5 games. The Battalion will host Guelph Sunday afternoon at the bunker.
Barrie continues to roll as they lead the Eastern Conference by 4 points over second place Mississauga. Last night they were able to jump out to a quick 2-0 lead at Oshawa and never gave up the lead. Camara and Beyers each had 2 goals in this contest. Goalie Neiderberger continues his steady play with 36 saves on 39 shots (including an impressive 20 saves in the third period). Last time these teams tangled was on Oct 25 where the Colts pushed by the Battalion in overtime for a 2-1 victory. The Colts do not play on Sunday, so look for Niederberger to continue to patrol the nets in this contest.
My prediction Brampton 0 Barrie 3
Sault Ste Marie at Owen Sound
The Greyhounds were in Kitchener last night where they continued to struggle. They have only won 3 of their past 10 games and currently sit in eighth place with a record of 9 wins, 9 losses and 1 overtime loss. The Rangers outshot them 42-22 – Staples had the only goal (powerplay marker late in the third period) to make the score 3-1 in favour of Kitchener. Nick Cousins had a quiet game with only 2 PIM on the scoresheet. He is tied for eighth in scoring with 8 goals and 19 assists this season (215 pts in 219 career OHL games).
Owen Sound was at Brampton to tangle with the Battalion in front of 2373 fans. Brandon Hope got the call in net to give Binnington a rest in preparation for tonight's tilt. Jordan Binnington will be looking for a bounce back game after giving up 2 goals in the Subway Super Series game on Thursday night. This provided Team Russia with their first win in the history of the international competition. The Attack's top line was buzzing last night – Catenacci with 2 goals and 1 assist, Gemel Smith with a goal and an assist. D-man Chiarlitti notched 3 assists and a + 3 rating (league leading +24 rating).
My prediction Owen Sound 5 Sault Ste Marie 2


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Please note:
- Beginning Thursday, June 11th, and continuing through August 13th, all Thursday classes will be cancelled for the summer.
- beYoga and Wellness will be closed from Saturday, July 25th, through Wednesday, August 5th.
beYoga and Wellness, founded in 2012 by Jennifer Baryluk. We are conveniently located in the Kingsdale area of beautiful Upper Arlington, Ohio, and are within easy walking distance to vibrant restaurants and shopping.
At beYoga and Wellness our focus is on restoring the body to balance with our selection of Hatha Yoga and Restorative Yoga classes. Our class schedule offers a full range of classes for the long time practitioner as well as those taking yoga for the first time. We also offer a full range of high quality, therapeutic grade Young Living Essential Oils that may complement your healthy lifestyle.
We invite you to experience yoga in our comfortable and relaxing space. Our hope is that our selection of classes and workshops will lead you to a path of healing and wholeness.


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The Masters
What Happens On The 12th Hole ...
Playing the 12th hole at Augusta is anything but predictable
Guy Yocom on the 12th Hole: "Swirling Down The Drain"
Ky Lafoon attempts to blast from the edge of Rae's Creek onto the 12th green.
- Keywords:
- Masters,
- golf,
- Augusta National,
- 12th hole,
- no. 12