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text:
FIEs pay CIT under investment license
Corporate income tax rates imposed on foreign-invested is usually regulated in the investment license. Under Official Letter 240/TCT/DTNN dated January 18, 2005 of the General Department of Taxation, in the case if CIT rate is not regulated in details at the investment license, FIEs must pay CIT under the prevailing regulations (at each time) of GDT. Reportedly, from January 1, 2004, the common CIT rate of 28% is applied to FIEs and local businesses.
[Official Letter 240/TCT/DTNN dated January 18, 2005]
Category: Legal

subset: commoncrawl


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Last month we took an impromptu trip to Chesapeake Bay. I've never had to pay to get onto a beach before, so excuse me if I'm still a bit perturbed. And by beach, please know that I am being incredibly generous. North Beach is in a part of Maryland I can't remember, just over an hour's drive from DC. The sand has so many rocks and shells it's impossible to walk barefoot. The water is green gray and smells like fish. I stepped in it up to my ankles and that was a feat. And did I mention we had to pay? I was livid. Pay to go on a non-beach? Just because you throw down sand and put it next to the water doesn't make it a beach. And it damn sure doesn't take away that nasty ass smell. I smelled Old Bay for two days after.
But! I was with family and the kids were happy, so that made the price, the smell, and the shells stabbing my feet worth it.
Seriously, my boy charmed everyone there with his pirateness. Hat? Check. Map? Check. Ship? Check. Eye patch? Check. Telling other kids not to climb up on his ship? Check more than once. Aaaargh, me maties.
The one you'd least expect to allow herself to be buried in sand was the one who offered to be buried in sand. Wonders never cease.
And the water. It smelled but its beauty wasn't lost (when you looked straight, far away, and saw nothing but water and sky).
My happiness this day was so extreme because it was easy. It was an easy day, no one was fighting, it wasn't too humid, and we enjoyed each other and were just happy to be together. My mother- and sister-in-law and niece were with us. It was simply one of those "let's go" days. I can't think of much better, or more happiness-inducing. And it's still summer. I cant, and won't, ask for more. Not yet.


text:
You should expect your child to have spelling homework each night. Math and Reading instruction is done daily and your child may receive that instruction from another 5th grade teacher. There will most likely will be assignments in each of those subjects.
Sometimes, I give class time to work on assignments, but not always. Be sure to check your child's Responsibility Sheet to check if they have homework or if all homework was received for that day. It should not take more than 45 minutes to complete ALL their homework. Please notify me if your child is having difficulty and is spending more than 45 minutes on homework.
Make up work
Students are expected to make up any missed assignments as soon as possible. It's each students responsibility to go to the absent work bin to get any papers/assignments they missed. It will be their responsibility to ask me for if they need any help with specific assignments.
If your child does not have me for reading, then it will be their responsibility to see their reading teacher for any missed assignments.
If a test is missed, it should be taken at the earliest lunch recess.


text:
Personalized Sisters Travel Coffee Mug - Personalized Sisters Heartstrings Travel MugReminisce about wonderful times spent with your sisters whenever you bring along your Personalized Sisters Heartstrings Travel Mug. Personalized Sister Travel Mugs are great for the woman who is always on the go. Give each sister a personalized Travel Mug to show them you will always be together, no matter where you are.
Our Custom Printed Sisters Travel Mug is dishwasher and microwave safe and holds 15oz. This sleek polymer travel mug includes a resilient plastic lid, lightweight and unbreakable. Includes FREE Personalization! Personalize your Sister Travel Mug with up to 6 names.
Your sisters are so important and their names are displayed elegantly on our personalized sisters mug. Buy them for each of your sisters to celebrate your love for each other. These pesonalized sisters mugs make excellent gifts for your sisters on Sister's Day, Family reunions or just a special girls day out.
We also carry many more Personalized Sister Gifts. Show your Sister how much you love her with our Personalized Sister Shirts & Sister Keepsakes!


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> News Article
New graduate program cited for talent development
Master's of Technical Entrepreneurship
(MTE) program has been honored by the University Economic Development Association (UEDA) as the best such program in North America for talent development.
The recognition
came after a team of faculty members made a presentation about the MTE program at UEDA's recent annual summit in Pittsburgh.
The MTE program trains students to create start-up companies and bring new products and services to market. The graduate program is offered by the P.C. Rossin College of Engineering and Applied Science and the
Baker Institute for Entrepreneurship, Creativity and Innovation
UEDA, a national organization of more than 100 universities and their economic development affiliates, encourages economic development by promoting innovation, entrepreneurship and higher education partnerships.
UEDA gave
2013 Awards for Excellence
in six categories at its summit. The Talent Development category recognized initiatives that promote development of 21st-century skills. Projects were judged on originality, sustainability, scalability and ability to be replicated.
Michael S. Lehman, MTE professor of practice, and John Ochs, MTE program director, gave the MTE presentation at the UEDA summit. Marc de Vinck, the Dexter F. Baker Professor of Practice in Creativity; Lisa Getzler-Linn, the Baker Institute's senior director for programs and operations; and Bill Michalerya, associate vice president for government relations and economic development and past president of UEDA, helped prepare the presentation.
From passion to accomplishment
The MTE program has grown rapidly in popularity since launching in May 2012. The first contingent of 14 students completed the program in May 2013. Within three months of graduating, six students had started their own businesses and seven had taken jobs in industry or academia.
In May 2013, 28 more students enrolled in the program.
MTE attracts students from a wide variety of backgrounds. Its students have earned bachelor's degrees in engineering, accounting, management, German, English, history, physics, behavioral neuroscience, political science, nanotechnology, global studies, journalism, psychology, finance, and art, architecture and design.
"This award is all about our students' accomplishments," said Ochs. "Our students embrace learning not because it's required to get a grade or a diploma, but because our program gives each person, regardless of their undergraduate degree, the opportunity to explore and develop their passion, with the final result being a new product or the company needed to bring that product to market.
"When people follow their passion in a supportive environment, great accomplishments follow."
"The UEDA award is a significant recognition for the MTE program, since the award is a competition for national best practices," said Michalerya. "This puts Lehigh on a national stage in a leadership role." It was Lehigh's
fourth win in the UEDA awards
and fifth time being a finalist, he said.
Lehman, who gave another presentation at the UEDA summit, said the talent development award brought visibility to the MTE program among the college faculty and administrators in attendance.
"We are having an impact regionally with the entrepreneurs who are starting companies and staying in the region," said Lehman. "We're having a national impact as well. Students are using their talents as they are employed by startups, existing companies, academia, nonprofits and government."
UEDA works to expand economic opportunity in communities by leveraging research, campus planning, community resources, talent development and technology commercialization. Its members represent higher education, private sector and community economic development stakeholders.
The MTE program is looking to double its enrollment again by in 2015, said Ochs.
"I am convinced that our model can and must be replicated at other universities in order to have the economic impact that follows student-led startups," he said. "As I like to say: 'Student innovation, fueled by creativity, is this generation's most important economic development engine.'"
Posted on Tuesday, January 14, 2014
Amy White
+ Enlarge
John Ochs (left) and Michael Lehman (center) accept the UEDA Talent Development award UEDA president Chuck Shoopman. (Photo courtesy of the University Economic Development Association)
The MTE program "gives students the opportunity to explore and develop their passion, with the final result being a new product or the company needed to bring that product to market."—John Ochs
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text:
While perusing my Pinterest Fashion Show at Lunch! board, I discovered I'd started a trend of pinning outfits including a denim jacket. Now, if you would have asked me straight up, "Amanda, would you enjoy a denim jacket?" I'd say to you, "Please go away and take your denim jacket talk back to the '80s where it belongs." But then I looked at my Pinterest board and was like, oh. I guess I do like denim jackets, and apparently enjoy seeing how to style a denim jacket. Pinspiration, people, it's a real thing.
One day I was thrifting and this amazing denim jacket, almost new, just my size, jumped in front of me and said "please buy me and make your Pinterest board a reality." Who is to argue with a talking thrift store jacket? Not me. (Check out this adorable denim jacket on Amazon for only $20!)
Wait, don't go. I know it's super hot outside and the thought of a denim jacket may make you break out in a spontaneous heat rash, but hear me out. I don't know about you, but my office is FREEZING. Like, bundle-up-in-a-sweater-and-blanket-and-USB-gloves kind of cold. The kind of cold where you long for the blistering heat of the outdoors and that glorious humidity. Why people can't get building temperatures right is beyond me. So, denim jackets are the perfect solution — heavy for the inside, removable for the outside.
Here are some tips for styling a denim jacket:
- Roll up the sleeves to give it a more "summery" look.
- Pair it with chunky jewelry so your jewelry isn't overpowered by the jacket.
- Layer with short sleeves or a tank top.
- Dresses are made for jean jackets — short dresses or maxis will both work.
- Style a denim jacket as your neutral and then color block your skirt and top or add a pop of color with accessories.
Here are some of my favorite ways to style a denim jacket for the bipolar temperatures that come with summer living.
Thrifted denim jacket, thrifted Express skirt, tshirt from Kohls, necklace vintage, earrings (the mall somewhere), bracelet from eBay. (Original Pinspiration for this outfit found here.)
Dress from Cotton On, belt from Body Central, necklace from Charming Charlie,
Dress from Loft via Goodwill; Necklace from eBay; earrings from Charming Charlie.
Tshirt from Kohls, refashioned skirt from a Goodwill polo, necklace from NY&Co, earrings from Premier Jewelry, cuff bracelet from the $5 sale in the atrium at work (similar one here for $11)
Not one of these outfits cost more than $10 total! I love thrifting.
Linking up with Amber of Real Girl Glam for her Real Girl Style linkup!


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During preconception and prenatal care checkups, providers have several screening and testing options available for women and their families. The March of Dimes Preconception and Prenatal Genetic Screening Pocket Facts describes widely used screening methods and data for certain birth defects:
Ordering pocket facts
To order, go to the genetics section of our online product catalog.
The following references are cited in the pocket facts:
Cystic fibrosis
Conditions more common in the Eastern European Jewish population
Fragile X
Screening for and diagnosis of fetal abnormalities during the prenatal period
Funding information
Funding for this web page is supported by Novartis Vaccines and Diagnostics. Novartis Vaccines and Diagnostics and the March of Dimes are working together to address the need for professional education for obstetricians, family physicians, nurses, nurse practitioners, certified nurse-midwives and genetic counselors to enhance their use of family history as a risk assessment tool for genetic conditions, such as birth defects, and improve their communication skills when speaking with their patients about prenatal screening and diagnosis.
The March of Dimes recommends that primary care clinicians receive education on how to offer genetic testing and screening to their patients and provide non-directive information that enables their patients, as prospective parents, to make decisions that are right for them as individuals, based on the patients' own informed understanding of both the nature and extent of risk to pregnancy outcome. The March of Dimes does not endorse specific brands or products.
Last reviewed August 2012
Yes. The March of Dimes produces fact sheets on several birth defects, including autism, chromosomal abnormalities, cleft lip, congenital heart defects and Down syndrome. Simply type the name of the birth defect into the search box.
A preconception checkup can help assure that a woman is as healthy as possible before she conceives. Her provider can identify and often treat health conditions that can pose a risk in pregnancy, such as high blood pressure, diabetes or certain infections. During the visit, the woman can learn about nutrition, weight, smoking, drinking alcohol and occupational exposures that can pose pregnancy risks. The provider also can make sure a woman's vaccinations are up to date and that any medications she takes are safe during pregnancy. The woman and her provider can discuss her health history and that of her partner and family. If the woman or her partner has a history of birth defects or preterm birth or if either has a high risk for a genetic disorder based on family history, ethnic background or age, the provider may suggest seeing a genetic counselor.
A birth defect is an abnormality of structure, function or metabolism (body chemistry) present at birth that results in physical or intellectual disabilities or death. Thousands of different birth defects have been identified. Birth defects are the leading cause of death in the first year of life.
See also: Common birth defects
The March of Dimes would like to see all babies in all states screened for at least 31 health conditions. Many of these health conditions can be treated if found early.
All states require newborn screening for at least 26 health conditions. Some states require screening for additional conditions – some up to 50 or more. For more information, read our article on newborn screening.


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- Why The Banner Ad is Heroic — enough to make Dave Eggers cry. Advertising triumphalism rampant.
- Udacity/Thrun Profile — A student taking college algebra in person was 52% more likely to pass than one taking a Udacity class, making the $150 price tag–roughly one-third the normal in-state tuition–seem like something less than a bargain. In which Udacity pivots to hiring-sponsored workforce training and the new educational revolution looks remarkably like sponsored content.
- Amazon is Building Substations (GigaOm) — the company even has firmware engineers whose job it is to rewrite the archaic code that normally runs on the switchgear designed to control the flow of power to electricity infrastructure. Pretty sure that wasn't a line item in the pitch deck for "the first Internet bookstore".
- Panoramic Images — throw the camera in the air, get a 360×360 image from 36 2-megapixel lenses. Not sure that throwing was previously a recognised UI gesture.
"MOOC" entries
Four short links: 19 November 2013
Ad Triumphalism, Education Not Transformed, Bookstore Infrastructure, and Tossable Camera
Semi-automatic method for grading a million homework assignments
Organize solutions into clusters and "force multiply" feedback provided by instructors
One of the hardest things about teaching a large class is grading exams and homework assignments. In my teaching days a "large class" was only in the few hundreds (still a challenge for the TAs and instructor). But in the age of MOOCs, classes with a few (hundred) thousand students aren't unusual.
Researchers at Stanford recently combed through over one million homework submissions from a large MOOC class offered in 2011. Students in the machine-learning course submitted programming code for assignments that consisted of several small programs (the typical submission was about 16 lines of code). While over 120,000 enrolled only about 10,000 students completed all homework assignments (about 25,000 submitted at least one assignment).
The researchers were interested in figuring out ways to ease the burden of grading the large volume of homework submissions. The premise was that by sufficiently organizing the "space of possible solutions", instructors would provide feedback to a few submissions, and their feedback could then be propagated to the rest.
Finding data after the shutdown, workarounds for reporters, and teaching a journalism MOOC.
When a government shutdown renders government data websites useless, what's a data journalist to do? This week, reporters hoping to gather data from sites like the US Census Bureau, the USDA's Food and Nutrition Service, and the Bureau of Economic Analysis were out of luck, as access to most online government data was blocked due to the government shutdown.
The Pew Research Center offered a mostly comprehensive list of the data casualties of the shutdown.
Data science in the public interest, 'digital media data gurus', and a comic about dirty data.
Insights and links from the data journalism beat
Data science in the public interest is en vogue, as collaborations between data scientists, nonprofits and human rights groups are springing up everywhere. Journalists at the Knight Foundation are following suit. This week, the foundation gave details about it's $2 million Knight News Challenge for health-related data projects. The "inspiration phase" launching next month invites citizens, journalists, and community groups anywhere in the world to dream up ideas about how to turn public data sets into useful information that could improve the health of communities.
Over at the Neiman Journalism Lab, a journalism professor writes that we are now entering the age of the "Digital Media Data Guru," a person with a hybrid of computer science and journalism skills who is able to "do it all" in the newsroom, and recommends that journalism schools prepare students for the data-centered work ahead of them.
Visualization of the Week: MOOC completion rates
Educational researcher Katy Jordan created an interactive visualization using completion and enrollment data from recent MOOCs.
Massive open online courses, or MOOCs, offered through platforms such as Coursera, EdX and Udacity, are arguably helping to fill higher education needs around the world. Educational researcher Katy Jordan noted in a post, however, that "although thousands enroll for courses, a very small proportion actually complete the course." To take a closer look, she pulled together an interactive visualization to show enrollment numbers and completion rates from recent MOOCs.
The MOOC movement is not an indicator of educational evolution
MOOCs get the attention, but DIY and peer-to-peer exchange are more fertile grounds for development
Somehow, recently, a lot of people have taken an interest in the broadcast of canned educational materials, and this practice — under a term that proponents and detractors have settled on, massive open online course (MOOC) — is getting a publicity surge. I know that the series of online classes offered by Stanford proved to be extraordinarily popular, leading to the foundation of Udacity and a number of other companies. But I wish people would stop getting so excited over this transitional technology. The attention drowns out two truly significant trends in progressive education: do-it-yourself labs and peer-to-peer exchanges.
In the current opinion torrent, Clay Shirky treats MOOCs in a recent article, and Joseph E. Aoun, president of Northeastern University, writes (in a Boston Globe subscription-only article) that traditional colleges will have to deal with the MOOC challenge. Jon Bruner points out on Radar that non-elite American institutions could use a good scare (although I know a lot of people whose lives were dramatically improved by attending such colleges). The December issue of Communications of the ACM offers Professor Richard A. DeMillo from the Georgia Institute of Technology assessing the possible role of MOOCs in changing education, along with an editorial by editor-in-chief Moshe Y. Vardi culminating with, "If I had my wish, I would wave a wand and make MOOCs disappear."
There's a popular metaphor for this early stage of innovation: we look back to the time when film-makers made the first moving pictures with professional performers by setting up cameras before stages in theaters. This era didn't last long before visionaries such as Georges Méliès, D. W. Griffith, Sergei Eisenstein, and Luis Buñuel uncovered what the new medium could do for itself. How soon will colleges get tired of putting lectures online and offer courses that take advantage of new media? Read more…
Will online learning destroy America's colleges?
Some parts of the American university system work well for their students. The rest are ready for disruption.
The American college system is staggeringly large: 2,421 four-year institutions enroll about 18.5 million college students. The proportion of Americans with a bachelor's degree is at an all-time high — a social victory if they're able to enjoy a positive return on their degrees, which the Pew Research Center estimates at about $550,000 on average.
And the very existence of that system is threatened, as we are to believe it, by the massive open online course, or MOOC, offered by new ventures from the likes of Stanford, Harvard and MIT. In an essay last week, Clay Shirky compared universities and MOOCs to record companies and Napster: in both cases, the incumbents operated by providing something inconveniently and locally that could be provided conveniently and universally on the web. I don't agree with the entire essay, but Shirky is absolutely right to point out that the college industry is made up of several markets, and they'll be disrupted in different ways.
American higher education is deeply divided: it's outstanding for a relative small handful of students and pretty bad for everyone else. The disruption of MOOCs will likely start at the bottom and move up from there. The question on which we should meditate is: how far up will it move? Read more…


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Tired? We hear you. And yoga can help. Practice these five yoga poses to give yourself a nice energy boost.
FITNESS GOAL: Energy
STYLE: Kundalini, or the science of breath and angles, teaches practitioners to avoid traditional static posing. Kundalini awakens cosmic energy believed to be dormant in all of us.
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EXPERT: Anna Getty, an instructor at Golden Bridge Yoga in Los Angeles, is the creator of a DVD series including The Divine Mother Prenatal Yoga.
Hold each pose for up to 20 minutes. The extended length and movement in each pose will build and sustain energetic highs. Keep eyes closed when you practice these poses. Get psyched up for a practice by chanting "Ong Namo Guru Dev Namo" (ong nah mo goo roo dayv nah mo)—"I bow to the creative wisdom in myself"—three times.
Spinal Flex/Camel Ride
Sit with legs crossed, holding onto calves or shins. Inhale and lift chest forward and up. Inhale and exhale as you round spine, chin to chest. Start slowly and increase pace steadily. Continue for three minutes. This movement releases blocked energy in the spine.
NEW from Prevention!
Get long and lean with sexy Flat Belly Yoga!
Sit with legs crossed. Place fingers (minus thumbs) on palms of opposite hands so fingertips are right below finger crease, on the mounds of hands. Leave thumbs open like you're hitchhiking. Bring hands overhead to a 60 degree angle. Breathe rapidly through nose, emphasizing exhale like a panting dog (this is Breath of Fire.) Hold for 1-3 minutes. To end, bring thumbs together overhead, open the remaining fingers, and release hands down.
Arm Pumps
Interlace fingers in front of you. Clasp hands so knuckles face out, palms in. Inhale, bring arms up and overhead. Exhale, lower hands down to knees. Continue this motion with a strong breath, inhaling arms up, exhaling arms down. Start with 3 minutes and build up to 7 minutes. To finish, inhale with arms up. Hold the breath for 15 seconds. Exhale and release hands down. The pumping motion stimulates energy flow from the spinal column to other parts of the body.
Stretch Pose
Sit on floor with legs outstretched. With each hand, grab onto corresponding big toe. (Or grab ankles or shins.) Inhale and arch back straight and up. Exhale, bend down at waist, and lower head to knees. Inhale, arch back up and straight, exhale, fold down at waist. Continue this motion at a steadily increased pace for 1 to 3 minutes. Briskly folding forward and backward encourages energy to distribute itself throughout the body. You might even feel a little dizzy.
Back Platform Pose
Sit on floor with legs in front of you. Place hands 6-12 inches behind butt, fingertips facing feet. Lift butt off floor. Squeezing butt, thighs, and calves, engage abs. Drop head back so you are in a straight line from top of head to tips of toes. Hold position and begin a rapid Breath of Fire. Continue for 1 minute. To finish, hold breath and posture for 15 seconds. Gently lower body to floor and relax in Corpse pose.


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Try adjusting you steering wheel pulled all the way out and see if that works for you, did me.
I noticed this too. After 1.5 yrs of ownership, decided to take it to a trusted alignment shop.
They told me subaru's run flat ( zero degrees camber on front end ).
They put some camber on it and it solved the problem for me. I can even drive with one hand now.
I found this wander very tiresome on 100 miles drives.
I was considering trading off if alignment didn't resolve.
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Washington County Budget Committee members were surprised last week when they learned the Board of Education in February instructed Director of Schools Ron Dykes to order six large school buses, two pickup trucks and a van at a cost of $638,000, an amount that now has popped up as an unbudgeted expense.
Though Dykes has said his understanding is that the $1.85 million in bonds approved in September by the County Commission would be used to reimburse the school system's transportation line item for buses, County Mayor Dan Eldridge and Budget Committee members don't remember it that way.
The method by which school bus replacement traditionally and legally transpires does allow for bonds to pay for such expenses. But in this case, the check for the buses —- which alone cost about $592,000 — was cut too early. Dykes, who was not immediately available for comment, initiated the purchase in July, just a few weeks too early for the bonds to be used to reimburse the buy, according to the Internal Revenue Service.
The calendar conundrum means the school system apparently is on the hook for that money and may be forced to carry more of a financial burden in the near future than expected.
"These are tax exempt bonds, and there are rigid restrictions," Eldridge said late Tuesday. "You cannot turn around and reimburse something that's already been paid for. The IRS has a thing called a 'Look Back' which gives you up to 60 days prior to the date on which the County Commission adopted its resolution. That still leaves us 13 days short.
"I mean how many opportunities were there to inform the Budget Committee? It never came up. We didn't know. That would have absolutely been an easy thing to fix."
Eldridge said it is now up to the Board of Education to decide how they want to handle the situation.
Budget Committee member Ethan Flynn said Tuesday that when the committee voted on the school system's budget, the expense for school buses was not included. Now, the school system faces an even tougher financial road because of the apparent gaffe.
"There's three pieces to this: the school buses, the half million dollars in school projects that were never in their budget and the projected sales tax shortfall," he said. "This could end up being $2 million to $3 million, because these are costs that must be shared with Johnson City."
The projected sales tax shortfall alone could leave the school system with a $1 million deficit. The school system also is staring at a series of unbudgeted projects totaling $450,000, such as the new roof for Fall Branch School and other capital projects. The unbudgeted buses alone total about $592,000.
Dykes said early this year that 19 large buses and five small buses would be purchased over the next three years, according to a state-established system of replacement after 16 years of service. In September, county commissioners approved the bond issuance to finance the turnover through three years.
This also means the county is in a position to financially stockpile four years' worth of buses by spring, but it's hardly a position they expected to have to fund.


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Top Dial-up Internet Service ProvidersJuly 31, 2015 - The best dial-up Internet service providers based on price, availability, features and support. Don't agree with the list? Vote for an existing item you think should be ranked higher or if you are a logged in, add a new item for others to vote on or create your own version of this list.
The Top Ten
As a six month customer of NetZero, I've had exceptional service! After a bad experience with other major ISPs, I turned to NetZero last year. Never regretted it yet. They even have great special offers for customers (I recently got two months of cost-free Internet access). I don't need a lot of frills, so NetZero works for me. I knew the speed would be a possible downer, but so far it's been reasonably fast. I researched NetZero's features first before signing-on, so I knew what I was getting into. It may not be for others, but for me it works.
I live in a rural area where only one access number is available. NetZero is the first dependable provider I have found where the number always works and isn't suddenly discontinued which happened with Toast and People PC.
One of the best provider..
2America Online
I used to have Netzero...but AOL has been a lot faster and better for me. And only $11.99 a month.
It's ok I love it
Had earthlink for 4 years and never had an issue... Easy to sign in and use.
Best I talked to. Haven't used anything yet
Years of great service.
I proud I m Muslim..
Visit ISP.com Website
5Northern Telephone
I like People PC a lot. It has always been fast, reliable and available wherever I would go. I used it for the past 7 years during the summer vacations and can recommend it very highly to anyone who lives in the woods, has power and phone and no cell coverage or DSL there. Before you sign up, always check if the access numbers. They have lots of them covering pretty much all the US. It never hurts to check The customer service is friendly and although has limited working hours (not 24/7) can help you to set everything up in no-time. Yes, and the Peoplepc.com prices are just right! Good luck!
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I have been using gobigwest for some time and have had no problems. The free phone support is great.
The thing about Sisna is that they meet my needs better than any other ISP, I've ever reviewed. And it's not by like a little bit.
It's so cool hardly ever breaks down the best among All of these in my opinion
The best out of all these ones I swear made by kim Dotcom himself
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This list was created 9 years, 257 days ago and has been voted on over 400 times. This top ten list has been remixed 1 times.
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Presenting New York's summer vacation wish list, and the other vacationers fulfilling it.
Stash lets you save events and search what others are doing.
The San Francisco-based Urban Eating League, a competitive eating event, is expanding to New York.
But repairs could take up to a week in places as debris from the storm still needs to be cleared.
Kevin Krim is trying to stay strong for his wife and surving child, his dad says.
Artists, engineers, scientists and more will head to Flushing Meadows-Corona Park this weekend.
In "The Glasshouse" the couple's art and home life are one, and open to the public.
While New Yorkers wait for the city's bike share, "Spinlister" offers by-owner rentals.
Floral prints, vibrant colors showcase New Yorkers' bohemian fashion sensibilities.
The Rental Car Rally, an overnight, costumed road trip from NYC to Montreal, hits the road next month.
The City of Brotherly Love took the top spot for bed bugs in a new study.
The tech giant unveiled new computers and software at their annual Worldwide Developer Conference.
Former Giants running back Brandon Jacobs agreed to a one-year contract with the San Francisco 49ers.
Super Bowl hero receiver Mario Manningham is leaving the Giants for the 49ers.
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Hi Guys,
I'm new here, but not new to knitting - more than 50 years experience.
I am not sure if I am allowed to tell you here that I run exchanges on another board, and you can join, if you so wish. The deadline for new members this month is 12midnight in hawaii, Thursday 10th April.
If we members here want to make our own Exchange Club, I would be very happy to organise it if necessary.
Best wishes, John
exchange, as in knit projects, or swapping unwanted yarn?
John runs the monthly knitting exchange over at Page By Page of Maui; it actually rotates through a number of crafts - and sometimes colours. It's an item exchange. knit, crochet, lace (any kind) ; etc.
the one closing tomorrow is "knit coaster" exchange and the next month is "crochet coaster" exchange.
MMario - ambiguity is cultivated, it doesn't happen in a vacuum!
Can't wait to see pictures of your lacework.
John is good people, people. And you should see his lacework! How many Forest Path stoles have you done, John?
MMario - ambiguity is cultivated, it doesn't happen in a vacuum!


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CHARLOTTE, NC - On behalf of SEIU's 2.1 million members and other working men and women across the country, SEIU President Mary Kay Henry will address Democratic National Convention delegates on Tuesday, Sept. 4, during the 6 p.m. hour.
Ms. Henry will focus on what's at stake for working people this election and the stark difference between President Obama's inclusive vision for America and Mitt Romney's plan to continue failed policies of the past.
WHO: SEIU President Mary Kay Henry to address DNC convention
WHEN: Tuesday, Sept. 4, during 6 p.m. hour
To arrange an interview with Ms. Henry during the DNC convention, contact Jenice R. Robinson (202.277.8750, email@example.com) or Mark McCullough (202.812.2528, firstname.lastname@example.com).


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|These Oprm1tm1Kff (or MOR-) knock-out mice exhibit a lack of morphine analgesia, reward, and withdrawal. These knock-out mice may be useful in studying the biological activity of opioids, analgesics, and responses to mechanical, chemical and thermal nociception at a supraspinal level.
Type Congenic; Targeted Mutation; Additional information on Genetically Engineered and Mutant Mice. Visit our online Nomenclature tutorial. Additional information on Congenic nomenclature. Mating System Homozygote x Homozygote (Female x Male) 05-AUG-09 Species laboratory mouse Generation N12+N1F15 (06-MAY-15)
Generation Definitions
Donating Investigator Brigitte L. Kieffer, Université Louis Pasteur Strasbourg
Mice homozygous for the mu-opioid receptor mutant allele Oprm1tm1Kff (or MOR-) are viable and fertile. MOR-selective ligand binding is absent on brain membranes from homozygous mice. Homozygous mice exhibit a lack of morphine analgesia, reward, and withdrawal. This is accompanied by decreased mechanical, thermal, and chemical pain thresholds. Homozygous mice also show decreased ethanol self-administration and decreased THC-conditioned place aversion. In contrast to mutant mice deficient of delta- or kappa-opioid receptors (Stock Nos. 007557 or 007558, respectively), Oprm1tm1Kff homozygotes exhibit hypolocomotive spontaneous stress responses. Indeed, the reduced anxiety and depressive-like behavior observed in Oprm1tm1Kff mutants is in stark contrast to kappa-opioid receptor deficient mice. These knock-out mice may be useful in studying the biological activity of opioids, analgesics, and responses to mechanical, chemical and thermal nociception at a supraspinal level.
In an attempt to offer alleles on well-characterized or multiple genetic backgrounds, alleles are frequently moved to a genetic background different from that on which an allele was first characterized. These mutant mice were originally published on a mixed genetic background. It should be noted that the phenotype could vary from that originally described. We will modify the strain description if necessary as published results become available.
A targeting vector was designed to insert a PGK-neo cassette into exon 2 of the targeted gene. The construct was electroporated into 129S2/SvPas-derived P1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts. The resulting chimeric mice were bred with C57BL/6 mice. These mu-opioid receptor (MOR) mutant mice were backcrossed to C57BL/6J inbred mice for at least 12 generations prior to arrival at The Jackson Laboratory. The Y chromosome may not have been fixed to the C57BL/6J genetic background.
|Considerations for Choosing Controls
Strains carrying other alleles of Oprm1
026221 B6.Cg-Oprm1tm1.1Jbwa/J 008750 B6.Cg-Tg(Th-Oprm1)4Jtw/JView Strains carrying other alleles of Oprm1 (2 strains)
View Related Disease (OMIM) TermsRelated Disease (OMIM) Terms provided by MGI
- Model with phenotypic similarity to human disease where etiologies are distinct. Human genes are associated with this disease. Orthologs of these genes do not appear in the mouse genotype(s).Autism
View Mammalian Phenotype TermsMammalian Phenotype Terms provided by MGIassigned by genotype
The following phenotype information is associated with a similar, but not exact match to this JAX® Mice strain.
Oprm1tm1Kff/Oprm1tm1Kffinvolves: 129S2/SvPas
- behavior/neurological phenotype
- abnormal pain threshold
- decreased behavioral withdrawal response
- prolonged morphine administration does not create symptoms of dependence or withdrawal (MGI Ref ID J:62551)
- hypoactivity
- mild change (MGI Ref ID J:36241)
- impaired behavioral response to anesthetic
- in a tail flick assay, mice treated with morphine-6beta-glucuronide (M6G) or 6-acetyl-morphine exhibit no analgesic response unlike similarly treated wild-type mice (MGI Ref ID J:52952)
- impaired behavioral response to morphine
- no affect on conditioned place preference induced by morphine no effect on conditioned place preference induced by morphine (MGI Ref ID J:36241)
- prolonged morphine administration does not create symptoms of dependence or withdrawal (MGI Ref ID J:36241)
- in a tail flick assay, mice treated with morphine-6beta-glucuronide (M6G) or 6-acetyl-morphine exhibit no analgesic response unlike similarly treated wild-type mice (MGI Ref ID J:52952)
- morphine-induced analgesia is abolished (MGI Ref ID J:207536)
- however, loperamine-induced analgesia is maintained (MGI Ref ID J:207536)
- increased coping response
- slightly decreased immobility time in forced-swim test (MGI Ref ID J:62551)
- integument phenotype
- abnormal pain threshold
Oprm1tm1Kff/Oprm1tm1Kffinvolves: 129S2/SvPas * C57BL/6J
- behavior/neurological phenotype
- abnormal social investigation
- males do not show a change in social exploratory activity when exposed to playback of female ultrasonic vocalizations unlike wild-type males that exhibit an increase of exploratory activity (MGI Ref ID J:204731)
- males however, exhibit normal behavior in the auditory cued-fear conditioning task (MGI Ref ID J:204731)View Research ApplicationsResearch ApplicationsThis mouse can be used to support research in many areas including:
Neurobiology Research
Behavioral and Learning Defects
genes regulating preferences to alcohol
genes regulating preferences to alcohol and stress
mu opioid receptor deficiency
Receptor Defects
Research Tools
Sensorineural Research
Toxicology Research
drug/compound testing
Sensorineural Research
|Allele Symbol
|Allele Name
|targeted mutation 1, Brigitte L Kieffer
|Allele Type
|Targeted (Null/Knockout)
|Common Name(s)
|Mutation Made By
|Brigitte Kieffer, Université Louis Pasteur Strasbourg
|Strain of Origin
|ES Cell Line Name
|ES Cell Line Strain
|Gene Symbol and Name
|Oprm1, opioid receptor, mu 1
|Gene Common Name(s)
|LMOR; M-OR-1; MOP; MOP receptor; MOP-R; MOR; MOR-1; MOR1; MORA; OPRM; Oprrm1; muOR;
|Molecular Note
|A neomycin selection cassette was inserted into exon 2. Binding assays on brain of homozygous animals confirmed that no functional protein was made from this allele. [MGI Ref ID J:36241]
Genotyping ProtocolsOprm1tm1Kff, Standard PCR
Helpful LinksGenotyping resources and troubleshooting
Martin M; Matifas A; Maldonado R; Kieffer BL. 2003. Acute antinociceptive responses in single and combinatorial opioid receptor knockout mice: distinct mu, delta and kappa tones. Eur J Neurosci 17(4):701-8. [PubMed: 12603260] [MGI Ref ID J:108005]
Matthes HW; Maldonado R; Simonin F; Valverde O; Slowe S; Kitchen I; Befort K; Dierich A; Le Meur M; Dolle P; Tzavara E; Hanoune J; Roques BP; Kieffer BL. 1996. Loss of morphine-induced analgesia, reward effect and withdrawal symptoms in mice lacking the mu-opioid-receptor gene [see comments] Nature 383(6603):819-23. [PubMed: 8893006] [MGI Ref ID J:36241]
Oprm1tm1Kff related
Berrendero F; Kieffer BL; Maldonado R. 2002. Attenuation of nicotine-induced antinociception, rewarding effects, and dependence in mu-opioid receptor knock-out mice. J Neurosci 22(24):10935-40. [PubMed: 12486188] [MGI Ref ID J:91861]
Bigliardi-Qi M; Gaveriaux-Ruff C; Pfaltz K; Bady P; Baumann T; Rufli T; Kieffer BL; Bigliardi PL. 2007. Deletion of mu- and kappa-opioid receptors in mice changes epidermal hypertrophy, density of peripheral nerve endings, and itch behavior. J Invest Dermatol 127(6):1479-88. [PubMed: 17185983] [MGI Ref ID J:121569]
Burbassi S; Sengupta R; Meucci O. 2010. Alterations of CXCR4 function in mu-opioid receptor-deficient glia. Eur J Neurosci 32(8):1278-88. [PubMed: 20880358] [MGI Ref ID J:169500]
Cendan CM; Pujalte JM; Portillo-Salido E; Montoliu L; Baeyens JM. 2005. Formalin-induced pain is reduced in sigma(1) receptor knockout mice. Eur J Pharmacol 511(1):73-4. [PubMed: 15777781] [MGI Ref ID J:101844]
Chefer VI; Denoroy L; Zapata A; Shippenberg TS. 2009. Mu opioid receptor modulation of somatodendritic dopamine overflow: GABAergic and glutamatergic mechanisms. Eur J Neurosci 30(2):272-8. [PubMed: 19614973] [MGI Ref ID J:151565]
Chefer VI; Kieffer BL; Shippenberg TS. 2003. Basal and morphine-evoked dopaminergic neurotransmission in the nucleus accumbens of MOR- and DOR-knockout mice. Eur J Neurosci 18(7):1915-22. [PubMed: 14622224] [MGI Ref ID J:89630]
Chefer VI; Kieffer BL; Shippenberg TS. 2004. Contrasting effects of micro opioid receptor and delta opioid receptor deletion upon the behavioral and neurochemical effects of cocaine. Neuroscience 127(2):497-503. [PubMed: 15262338] [MGI Ref ID J:91973]
Chen Y; Kong S; Tang X; Fu Y; Wang B; Zhang S; Wang H. 2014. Preimplantation mouse embryo is a target for opioid ligand-receptor signaling. Biol Reprod 91(1):4. [PubMed: 24855103] [MGI Ref ID J:213203]
Contet C; Matifas A; Kieffer BL. 2004. No evidence for G-protein-coupled epsilon receptor in the brain of triple opioid receptor knockout mouse. Eur J Pharmacol 492(2-3):131-6. [PubMed: 15178356] [MGI Ref ID J:101864]
Cui Y; Ostlund SB; James AS; Park CS; Ge W; Roberts KW; Mittal N; Murphy NP; Cepeda C; Kieffer BL; Levine MS; Jentsch JD; Walwyn WM; Sun YE; Evans CJ; Maidment NT; Yang XW. 2014. Targeted expression of mu-opioid receptors in a subset of striatal direct-pathway neurons restores opiate reward. Nat Neurosci 17(2):254-61. [PubMed: 24413699] [MGI Ref ID J:207986]
Dahan A; Sarton E; Teppema L; Olievier C; Nieuwenhuijs D; Matthes HW; Kieffer BL. 2001. Anesthetic potency and influence of morphine and sevoflurane on respiration in mu-opioid receptor knockout mice. Anesthesiology 94(5):824-32. [PubMed: 11388534] [MGI Ref ID J:106575]
Filliol D; Ghozland S; Chluba J; Martin M; Matthes HW; Simonin F; Befort K; Gaveriaux-Ruff C; Dierich A; LeMeur M; Valverde O; Maldonado R; Kieffer BL. 2000. Mice deficient for delta- and mu-opioid receptors exhibit opposing alterations of emotional responses. Nat Genet 25(2):195-200. [PubMed: 10835636] [MGI Ref ID J:62551]
Garcia-Sevilla JA; Ferrer-Alcon M; Martin M; Kieffer BL; Maldonado R. 2004. Neurofilament proteins and cAMP pathway in brains of mu-, delta- or kappa-opioid receptor gene knock-out mice: effects of chronic morphine administration. Neuropharmacology 46(4):519-30. [PubMed: 14975676] [MGI Ref ID J:97004]
Gaveriaux-Ruff C; Filliol D; Simonin F; Matthes HW; Kieffer BL. 2001. Immunosuppression by delta-opioid antagonist naltrindole: delta- and triple mu/delta/kappa-opioid receptor knockout mice reveal a nonopioid activity. J Pharmacol Exp Ther 298(3):1193-8. [PubMed: 11504820] [MGI Ref ID J:126857]
Gaveriaux-Ruff C; Karchewski LA; Hever X; Matifas A; Kieffer BL. 2008. Inflammatory pain is enhanced in delta opioid receptor-knockout mice. Eur J Neurosci 27(10):2558-67. [PubMed: 18513322] [MGI Ref ID J:137050]
Gaveriaux-Ruff C; Matthes HW; Peluso J; Kieffer BL. 1998. Abolition of morphine-immunosuppression in mice lacking the mu-opioid receptor gene. Proc Natl Acad Sci U S A 95(11):6326-30. [PubMed: 9600964] [MGI Ref ID J:48241]
Gaveriaux-Ruff C; Simonin F; Filliol D; Kieffer BL. 2003. Enhanced humoral response in kappa-opioid receptor knockout mice. J Neuroimmunol 134(1-2):72-81. [PubMed: 12507774] [MGI Ref ID J:119182]
Ghozland S; Chu K; Kieffer BL; Roberts AJ. 2005. Lack of stimulant and anxiolytic-like effects of ethanol and accelerated development of ethanol dependence in mu-opioid receptor knockout mice. Neuropharmacology 49(4):493-501. [PubMed: 15961126] [MGI Ref ID J:106610]
Ghozland S; Matthes HW; Simonin F; Filliol D; Kieffer BL; Maldonado R. 2002. Motivational effects of cannabinoids are mediated by mu-opioid and kappa-opioid receptors. J Neurosci 22(3):1146-54. [PubMed: 11826143] [MGI Ref ID J:127005]
Jamot L; Matthes HW; Simonin F; Kieffer BL; Roder JC. 2003. Differential involvement of the mu and kappa opioid receptors in spatial learning. Genes Brain Behav 2(2):80-92. [PubMed: 12884965] [MGI Ref ID J:104908]
Laurent V; Leung B; Maidment N; Balleine BW. 2012. mu- and delta-opioid-related processes in the accumbens core and shell differentially mediate the influence of reward-guided and stimulus-guided decisions on choice. J Neurosci 32(5):1875-83. [PubMed: 22302826] [MGI Ref ID J:181339]
Lena I; Matthes H; Kieffer B; Kitchen I. 2004. Quantitative autoradiography of dopamine receptors in the brains of micro-opioid receptor knockout mice. Neurosci Lett 356(3):220-4. [PubMed: 15036634] [MGI Ref ID J:121034]
Majumdar S; Grinnell S; Le Rouzic V; Burgman M; Polikar L; Ansonoff M; Pintar J; Pan YX; Pasternak GW. 2011. Truncated G protein-coupled mu opioid receptor MOR-1 splice variants are targets for highly potent opioid analgesics lacking side effects. Proc Natl Acad Sci U S A 108(49):19778-83. [PubMed: 22106286] [MGI Ref ID J:180448]
Marquez P; Baliram R; Kieffer BL; Lutfy K. 2007. The mu opioid receptor is involved in buprenorphine-induced locomotor stimulation and conditioned place preference. Neuropharmacology 52(6):1336-41. [PubMed: 17367825] [MGI Ref ID J:124471]
Matthes HW; Smadja C; Valverde O; Vonesch JL; Foutz AS; Boudinot E; Denavit-Saubie M; Severini C; Negri L; Roques BP; Maldonado R; Kieffer BL. 1998. Activity of the delta-opioid receptor is partially reduced, whereas activity of the kappa-receptor is maintained in mice lacking the mu-receptor. J Neurosci 18(18):7285-95. [PubMed: 9736649] [MGI Ref ID J:120239]
Matynia A; Parikh S; Chen B; Kim P; McNeill DS; Nusinowitz S; Evans C; Gorin MB. 2012. Intrinsically photosensitive retinal ganglion cells are the primary but not exclusive circuit for light aversion. Exp Eye Res 105:60-9. [PubMed: 23078956] [MGI Ref ID J:203664]
Melief EJ; Miyatake M; Bruchas MR; Chavkin C. 2010. Ligand-directed c-Jun N-terminal kinase activation disrupts opioid receptor signaling. Proc Natl Acad Sci U S A 107(25):11608-13. [PubMed: 20534436] [MGI Ref ID J:161284]
Morin-Surun MP; Boudinot E; Dubois C; Matthes HW; Kieffer BL; Denavit-Saubie M; Champagnat J; Foutz AS. 2001. Respiratory function in adult mice lacking the mu-opioid receptor: role of delta-receptors. Eur J Neurosci 13(9):1703-10. [PubMed: 11359522] [MGI Ref ID J:89400]
Nieto MM; Guen SL; Kieffer BL; Roques BP; Noble F. 2005. Physiological control of emotion-related behaviors by endogenous enkephalins involves essentially the delta opioid receptors. Neuroscience 135(2):305-13. [PubMed: 16112476] [MGI Ref ID J:104438]
Oakley SM; Toth G; Borsodi A; Kieffer BL; Kitchen I. 2003. G-protein coupling of delta-opioid receptors in brains of mu-opioid receptor knockout mice. Eur J Pharmacol 466(1-2):91-8. [PubMed: 12679145] [MGI Ref ID J:102598]
Oddi D; Crusio WE; D'Amato FR; Pietropaolo S. 2013. Monogenic mouse models of social dysfunction: Implications for autism. Behav Brain Res :. [PubMed: 23327738] [MGI Ref ID J:197157]
Olmstead MC; Ouagazzal AM; Kieffer BL. 2009. Mu and delta opioid receptors oppositely regulate motor impulsivity in the signaled nose poke task. PLoS ONE 4(2):e4410. [PubMed: 19198656] [MGI Ref ID J:146471]
Papaleo F; Kieffer BL; Tabarin A; Contarino A. 2007. Decreased motivation to eat in mu-opioid receptor-deficient mice. Eur J Neurosci 25(11):3398-405. [PubMed: 17553008] [MGI Ref ID J:126072]
Roberts AJ; McDonald JS; Heyser CJ; Kieffer BL; Matthes HW; Koob GF; Gold LH. 2000. mu-Opioid receptor knockout mice do not self-administer alcohol. J Pharmacol Exp Ther 293(3):1002-8. [PubMed: 10869404] [MGI Ref ID J:120530]
Robledo P; Mendizabal V; Ortuno J; de la Torre R; Kieffer BL; Maldonado R. 2004. The rewarding properties of MDMA are preserved in mice lacking mu-opioid receptors. Eur J Neurosci 20(3):853-8. [PubMed: 15255997] [MGI Ref ID J:100339]
Samaco RC; Mandel-Brehm C; McGraw CM; Shaw CA; McGill BE; Zoghbi HY. 2012. Crh and Oprm1 mediate anxiety-related behavior and social approach in a mouse model of MECP2 duplication syndrome. Nat Genet 44(2):206-11. [PubMed: 22231481] [MGI Ref ID J:181213]
Sanders MJ; Kieffer BL; Fanselow MS. 2005. Deletion of the mu opioid receptor results in impaired acquisition of Pavlovian context fear. Neurobiol Learn Mem 84(1):33-41. [PubMed: 15936681] [MGI Ref ID J:103799]
Scherrer G; Befort K; Contet C; Becker J; Matifas A; Kieffer BL. 2004. The delta agonists DPDPE and deltorphin II recruit predominantly mu receptors to produce thermal analgesia: a parallel study of mu, delta and combinatorial opioid receptor knockout mice. Eur J Neurosci 19(8):2239-48. [PubMed: 15090050] [MGI Ref ID J:89672]
Schuller AG; King MA; Zhang J; Bolan E; Pan YX; Morgan DJ; Chang A ; Czick ME ; Unterwald EM ; Pasternak GW ; Pintar JE. 1999. Retention of heroin and morphine-6 beta-glucuronide analgesia in a new line of mice lacking exon 1 of MOR-1. Nat Neurosci 2(2):151-6. [PubMed: 10195199] [MGI Ref ID J:52952]
Skoubis PD; Matthes HW; Walwyn WM; Kieffer BL; Maidment NT. 2001. Naloxone fails to produce conditioned place aversion in mu-opioid receptor knock-out mice. Neuroscience 106(4):757-63. [PubMed: 11682161] [MGI Ref ID J:126648]
Wamsteeker Cusulin JI; Fuzesi T; Inoue W; Bains JS. 2013. Glucocorticoid feedback uncovers retrograde opioid signaling at hypothalamic synapses. Nat Neurosci 16(5):596-604. [PubMed: 23563581] [MGI Ref ID J:197687]
Weibel R; Reiss D; Karchewski L; Gardon O; Matifas A; Filliol D; Becker JA; Wood JN; Kieffer BL; Gaveriaux-Ruff C. 2013. Mu opioid receptors on primary afferent nav1.8 neurons contribute to opiate-induced analgesia: insight from conditional knockout mice. PLoS One 8(9):e74706. [PubMed: 24069332] [MGI Ref ID J:207536]
Wohr M; Moles A; Schwarting RK; D'Amato FR. 2011. Lack of social exploratory activation in male mu-opioid receptor KO mice in response to playback of female ultrasonic vocalizations. Soc Neurosci 6(1):76-87. [PubMed: 20503133] [MGI Ref ID J:204731]
Animal Health ReportsRoom Number FGB27
Colony Maintenance
Breeding & Husbandry When maintaining a live colony, heterozygous or homozygous mice may be bred. Mating System Homozygote x Homozygote (Female x Male) 05-AUG-09 Diet Information LabDiet® 5K52/5K67
|Pricing for USA, Canada and Mexico shipping destinations
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $232.00 Female or Male Homozygous for Oprm1tm1Kff
Price per Pair (US dollars $) Pair Genotype $464.00 Homozygous for Oprm1tm1Kff x Homozygous for Oprm1tm1Kff
Standard Supply
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.
|Pricing for International shipping destinations
Price per mouse (US dollars $) Gender Genotypes Provided Individual Mouse $301.60 Female or Male Homozygous for Oprm1tm1Kff
Price per Pair (US dollars $) Pair Genotype $603.20 Homozygous for Oprm1tm1Kff x Homozygous for Oprm1tm1Kff
Standard Supply
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.
Repository-Live represents an exclusive set of over 1800 unique mouse models across a vast array of research areas. Breeding colonies provide mice for large and small orders and fluctuate in size depending on current research demand. If a strain is not immediately available, you will receive an estimated availability timeframe for your inquiry or order in 2-3 business days. Repository strains typically are delivered at 4 to 8 weeks of age. Requests for specific ages will be noted but not guaranteed and we do not accept age requests for breeder pairs. However, if cohorts of mice (5 or more of one gender) are needed at a specific age range for experiments, we will do our best to accommodate your age request.
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|Control Pricing Information for Genetically Engineered Mutant Strains.
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Scotts House , second oldest building in Boldon is a short distance from West Boldon on the Newcastle road. Scots House is mentioned as long ago as 1381, when Richard Hedworth died possessed of Scottes House on Boldon Moor.
The tenure of the house was passed on to William, son of Gilbert of Boldon. The Coles of Gateshead became its owners in 1617, and later in 1658, came into the possession of Henry Maddison, and from him to the Hudson family followed by the Wades.
It was later occupied by Hugh Lee Pattison, founder of the Washington Chemical Co. and later tenanted by George Charlton who farmed the surrounding land. The house stood empty for a short while but is now occupied, being converted into flats.
Little of the original building is now standing, the oldest part of the present structure dates to about 1620 and the whole is a mixture of restoration and alteration spread over the last 300 years. The house is still a fine looking mansion although blackened by age and stands on the south side of the main road at West Boldon.
At the entrance to the drive leading up to the house is a gate cottage which, up to the last century was a toll cottage because the Newcastle -Sunderland road was private and all traffic had to pay toll to pass.


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We are currently installing new doors in the stairwell in Lauinger Library. During this time, visitors will not be able to use landings that are under construction, either to enter that floor or pass through en route to another. We encourage visitors to use the elevators, although the stairwell may still be used to access floors that are not under construction. Landings under construction should only be used in the case of an emergency.
Paul Betz, Professor of English, with an introduction by Marty Barringer, Associate University Librarian for Special Collections


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Full detailed profile on Little Ethan Crane Winthrop Played by Colton Shires on Passions Daytime Soap Opera.
Colton Shires
Birthday: October 16 1996
Ethan and Gwen get dressed up to head to the Blue Note while Theresa is in Little Ethan's room without her Gertrude costume on. Little E asks if it's a good idea to be out of her costume and Theresa tells him she has a plan. She changes into a new disguise so she can go to The Blue Note and spy on Gwen and Ethan. She puts on a blond wig and little black dress and asks Little Ethan if anyone would recognize her. He assures her that he almost didn't recognize her!
In their bedroom, Ethan and Gwen try to relieve the itching and Ethan trips over the table of food sending everything flying. Little Ethan tells them Jane is crying and Theresa laughs in the hallway over how well her plan is working. Little Ethan asks if Gwen and Ethan are going to help Jane and Gwen snaps that she'll get to her when she can. Ethan tells Gwen to ease off Little E, but Gwen continues to be a diva about the situation. Jane stops crying and Gertrude comes in the room saying she got Jane back to sleep. She asks why they are itching and why food is all over the floor and Ethan says it's a long story. Gertrude tells Gwen she'll clean up the mess so she can shower and Gwen is grateful. While Gwen showers, Ethan continues to itch and Gertrude offers to help by scratching his back. Ethan is relieved and asks, "What would I do without you?" Theresa thinks to herself he will never have to find out because she's back and here to stay.
Theresa puts Little Ethan to bed and he asks how she's going to make it so Ethan isn't with Gwen anymore. Theresa doesn't know, but reminds him that for now everyone needs to think she's just the nanny. She promises that one day they will all be a family together and kisses him goodnight.
In Gwen and Ethan's room, Little Ethan asks Theresa why she came back dressed as a nanny. She tells him she'll fill him in later, but right now no one can know she's alive. Gwen and Ethan talk in the hallway and remark how great Gertrude is, while Little Ethan promises Theresa to keep the secret. Little Ethan hugs his mother, as Ethan and Gwen walk in the room. Ethan sees them and tells Gwen there's something familiar about Gertrude that makes him feel like he's known her forever. Gwen doesn't get it and Gertrude says Little Ethan has something to show them. He pulls out some cards and does a magic trick. Theresa thinks to herself, "Enjoy it now Gwen, because you won't have Ethan for long. I'll make sure of it." Little Ethan does another trick and Gertrude says it's time for bed. Little Ethan asks if Ethan would like to tuck him in and he says, "You bet" and leaves with him and Gertrude.
Theresa leaves the room and runs into Ethan. Still dressed as Gertrude, she asks if she can leave the house to go get the rest of her things. He says of course and enters his room. Gwen comes out in a negligee and Ethan is delighted by her surprise. They start to kiss as Theresa listens at the door eagerly awaiting her sabotage to take effect. Ethan and Gwen sit at the table and feed each other. Suddenly Ethan yells out that his mouth is on fire and then Gwen feels it too! They down some water and decide not to eat any more food. They move to the bed and roll around in the sheets, but they start to itch uncontrollably and jump out of bed. They try to relive their itching, as Little Ethan comes in the room saying that Jane is crying. Ethan says he'll take care of it because he let Gertrude leave to gather her things. He then trips and knocks over the table full of their food as Jane continues to cry. Ethan jokes that Gwen did promise him a night he wouldn't forget, as Theresa hides in the hallway laughing.
At the mansion, Gwen and Ethan tell the disguised Theresa all about the children. Little Ethan stares at Theresa, wondering where he's seen this bizarre looking woman before. Ethan informs her that Little Ethan's mother recently died and the boy suddenly blurts out, "Mom!" Ethan is baffled by this. Gertrude explains that the boy is just answering a question she asked him earlier; he was just telling her that he got his eyes from his mother. Rebecca hopes that's all he got from Theresa and thinks Gertrude should be checked for lice. Ethan sends her out to wait in the hall while they make a decision. He thinks she's perfect and the women go along with it. Ethan kneels down and asks his son if he's okay with this. "I think Gertrude will be the best nanny ever," he says. That's good enough for Ethan so they call Gertrude back in to tell her the news.
As the adults leave to prepare a list of her responsibilities, Little Ethan asks Gertrude if she is really Theresa. She admits it, but tells him that they have to keep this a secret. She spends some time looking at his homework before the boy runs off to get something. Ethan sits down with her and tells him how happy he is that they are getting along. He explains that he is adopting the boy and he needs the boy to get extra attention to cope with losing his mother. The children are all that he has left of Theresa, he says sadly while Theresa puts her hand on his knee. She pulls away and apologizes. He says it's okay and she asks him to tell her what Theresa was like. "She was destructive... like a force of nature... but beautiful on so many levels... I don't think I'll ever get over her," he says, unable to understand why he is opening up to Gertrude like this. "I feel like I've known you for a long time," he says. She promises to love his children like her own flesh and blood. Little Ethan returns and takes her to his room.
At the Crane Mansion, Pilar tells Ethan she has something to tell him about his relationship with Little Ethan. Before she can spit out that he is Little E's father, Gwen comes in the room interrupting them. Ethan tells her Pilar has something important to say, but Gwen says Little E needs him right now and Ethan leaves the room. Gwen demands to know what Pilar is doing and Pilar tells her Ethan deserves to know the truth about his son. Gwen insists she won't let that happen and threatens to call Juanita if she tells Ethan the truth. Ethan returns and says Little E didn't need him and asks Pilar what she wanted to tell him. Pilar thinks to herself that Gwen really will tell Juanita where her family is and then they'll all die, so Pilar tells Ethan that Little E is emotionally fragile and that he needs to be careful with him. Ethan promises to always be a good father to him because he loves him as his own. Little Ethan enters the room and Ethan suggests they go raid the refrigerator. Gwen, Ethan and Little E leave and Pilar thinks that Gwen will get hers sooner than she thinks.
As Ivy and Rebecca go over the rules of the house with Gertrude, Ethan, Little E and Gwen come in the room. Little E goes to the kitchen and Ivy and Rebecca introduce Gertrude to Ethan and Gwen. Ethan shakes her hand and asks if they know each other. Gertrude says she doesn't know him, but Ethan gets a flash of Theresa's face. Ethan says she reminds him of someone...an old friend. Gwen pulls Rebecca and Ivy aside and asks where they found such an ugly nanny. They explain Ethan will never be distracted by her, but Gwen wonders if she will be good at her job. Pilar enters the room with Jane, and Gwen takes the little girl in her arms. Gwen tells Gertrude that Jane has a hard time with strangers, but Gertrude asks if she can hold her. Gertrude takes Jane and everyone marvels at how at ease Jane is with her. Gwen is shocked because Jane never likes strangers and Ethan says the only other person that could put Jane at ease was...and thinks "Theresa." He says Gertrude reminds him of Theresa because of her nurturing way. Pilar introduces herself to Gertrude, takes Jane back and heads upstairs with her. Little Ethan returns with a sandwich and he is introduced to Gertrude. He asks, "Haven't I seen you before?" Gertrude says maybe she was a nanny to one of his friends, but Little E doesn't think that's it. Ethan and Gwen talk to Gertrude, while Little E wonders where he's seen her before. He suddenly blurts out, "Mom!"
Ethan talks to Little Ethan and apologizes for all the commotion. Little E asks if Gwen really saw his mom, but Ethan tells him no. Little E is sorry that Ethan didn't get to see Theresa like he and Gwen did. Ethan assures him that his mom is gone, but Little E says it was so real. Ethan says he wishes it were, while Theresa listens outside the window and says it's nice to know Ethan still loves her. Both Ethan and Little E promise to always be there for each other.
« Back to Little Ethan Crane Winthrop profile


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HAZARD — For Melissa Stidham, the decision to pursue a remote-work customer service and sales job with the help of Kentucky Teleworks was an easy one.
A self-confessed "people person who loves to talk," Stidham jumped at the chance for such a job. She also became one of the first employees to work full-time at the service's new Teleworks Hub in Hazard.
Stidham is now employed by StarTek, a call-center, customer-service vendor for a major U.S. telephone, Internet and satellite TV provider, and said she couldn't be happier.
"These kinds of jobs are the future of the workforce in Eastern Kentucky," she said. "The pay and the benefits are great, and I love the fact that I got this opportunity and didn't have to leave my hometown."
Others looking to join Stidham can apply for customer service positions with StarTek at a series of Kentucky Teleworks job screenings in July coming to:
Hazard – Tuesday and Wednesday, July 9-10 – Kentucky Career Center Perry County JobSight (Teleworks Hub location), 412 Roy Campbell Drive
Prestonsburg – Thursday, July 11 – Kentucky Career Center, 686 North Lake Drive
Manchester – Friday, July 12 – Clay County JobSight at Daniel Boone Community Action Agency, 1535 Shamrock Road
All events will take place from 10 a.m. – 4 p.m. at each location.
Applicants interested in being screened on-site at the events should bring a current resume, valid identification card, and Social Security card.
Successful candidates can choose to either telework from an office in their home or—like Stidham—at the Teleworks Hub located at the Kentucky Career Center Perry County JobSight in Hazard.
Jobs with StarTek feature:
• Fully paid training
• Medical, dental, vision and life insurance plans
• Paid holidays, paid overtime and paid time off
• Variety of work schedules
• Incentive pay programs
• Comfortable business casual dress and environment
• Employee referral bonus programs
• Flexible spending accounts for medical and dependent care
Eastern Kentuckians who were employed at former area call centers like Sykes Enterprises, ACS, and others are prime candidates for telework job opportunities with StarTek, said Kentucky Teleworks Director Joshua Ball.
Additionally, those selected for positions with StarTek from the July screenings could be hired and start training as soon as August.
"In the world of telework, it's about bringing the people to the jobs instead of bringing the jobs to the people," Ball said. "These jobs give those who worked in the customer service field the chance to once again utilize their skills right here in the region where they live, and with a very quick turnaround from their applying to potentially being hired."
Applying through Kentucky Teleworks also gives applicants lacking high-speed Internet access or appropriate computer equipment for a home office the opportunity to be stationed at the Teleworks Hub in Hazard. There, teleworkers have access to all of those items in 44 workstations in 22 comfortable offices.
A service affiliated with the Eastern Kentucky Concentrated Employment Program, Inc. (EKCEP)—the region's workforce development organization—Kentucky Teleworks identifies and develops valid work-from-home opportunities and helps people prepare for and land these jobs.
Through its website, www.kentuckyteleworks.com, jobseekers have access to thousands of additional vetted work-from-home jobs. In addition to StarTek, Kentuckians have landed jobs with well-known national companies like DirecTV, Apple, Amazon.com, T-Mobile, Comcast, JP Morgan Chase and others.
Melissa Stidham said she's excited that more Eastern Kentuckians will join the ranks of StarTek through Kentucky Teleworks and its July job screening events.
"We have job opportunities we didn't have before," Stidham said. "You get a sense of confidence because you don't have to leave your hometown to be a part of what's becoming a thriving workforce."
For more information about Kentucky Teleworks, visit www.kentuckyteleworks.com; call Josh Ball at (606) 435-8498, or email email@example.com. Find Kentucky Teleworks on Facebook at www.facebook.com/kentuckyteleworks and EKCEP at www.facebook.com/ekcep.
More information on StarTek careers, visit the company online at http://www.startek.com/us-careers.


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- Be the first to Review
Your rating for Kahanu Gardens
Expert Review of Kahanu Gardens
Active and Adventure Review of Kahanu Gardens
Active and AdventureExpert Tips & Helpful Hints
- The park also contains the largest heiau in all of Hawaii, the Hale O Pi`ilani Heiau, a stone structure that was once lost to the jungle.
- View plants brought to Hawaii from Polynesia by the first settlers about 2,000 years ago.
(First left past mile marker 31 on Hana Hwy)
Ula'ino Rd
Hana, Hawaii 96713
(First left past mile marker 31 on Hana Hwy)
Ula'ino Rd
Hana, Hawaii 96713
Parking: M-F 10a-2p; Reservations required
Visitor Information
Telephone Number: +1 808 248 8912
Neighborhood: Hana / Haleakala
The details, dates, and prices mentioned here were accurate at the time of publication.
Review by Away Copyright © 2010 Away.com all rights reserved.
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Google displays Lincoln's handwritten Gettysburg Address
In honor of the 150th anniversary of the famous speech, a Google exhibit lets people view one of the five known copies in Abraham Lincoln's handwriting.
Google offers us a glimpse 150 years into the past with a new exhibit celebrating the anniversary of Abraham Lincoln's Gettysburg Address.
On November 19, 1863, President Lincoln delivered the short but famous speech that began with the words "Four score and seven years ago..." and then went on to commemorate the soldiers who lost their lives at the Battle of Gettysburg and the principles upon which the United States was formed.
Five known copies of the speech in Lincoln's handwriting exist, according to a Web site devoted to the 16th president. Google's new exhibit offers a look at one of those speeches, known as the Bancroft Copy.
Through Google's online exhibit, you can zoom in on every word of Lincoln's speech. The exhibit also displays the letter that the President wrote to George Bancroft, an American historian and diplomat, who had requested a copy of the speech. Documents and images about the Gettysburg Address complete the exhibit in an attempt to provide insight into one of the most celebrated speeches in American history.


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