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Multiwell plates commonly used for immunoassay are increased in capacity and adapted for ease and speed of testing by forming a plurality of solid posts in each well of a plate. The posts and plate material and the dimensions of the posts are chosen to allow the immobilization of ligand patterns on an exterior wall of a post in a well and to permit a collimated beam of light directed to the post in a direction to achieve total internal reflection from a wall to generate an evanescent field in the plane of the ligands immobilized on the exterior wall of the post. The reflected light carries an image of localized intensity variations due to binding events between the ligand patterns and analytes in a sample introduced into a well. A cover plate seals the wells and provides for through holes for introducing sample material to the wells.
1. An apparatus, comprising:
a first transparent planar plate having at least one well therein, the well including a plurality of transparent upstanding posts therein,
wherein each of the plurality of posts has a planar sidewall and is of a material and has cross-sectional dimensions and a height to permit a beam of light directed into the post to produce total internal reflection in a manner to produce an evanescent field at the sidewall.
2. The apparatus as in
3. The apparatus as in
4. The apparatus as in
5. The apparatus as in
6. An apparatus, comprising:
a first transparent planar plate having at least one well therein, the well including a plurality of transparent upstanding posts therein, wherein the posts are coated with a ligand; and
a second transparent plate juxtaposed with the first plate in a manner to seal the well wherein the second plate includes a hole aligned with the well.
7. The apparatus as in
8. The apparatus as in
9. The apparatus as in
10. An apparatus, comprising:
a first transparent planar plate having a plurality of wells, each of the wells comprising a plurality of transparent upstanding posts organized in a plurality of arrays wherein the posts of each array is coated with a different ligand,
wherein each of the posts in each of the wells has a planar sidewall and is of a material and has cross sectional dimensions and a height to permit a beam of light directed into a post to produce total internal reflection in a manner to produce an evanescent field at the sidewall of the post.
11. The apparatus as in
12. An apparatus, comprising:
a first transparent plate having at least one well therein, the well including at least one upstanding post, wherein the at least one upstanding post is coated with a ligand; and
a second plate juxtaposed with the first plate in a manner to seal the well, wherein the second plate includes a through hole for introducing a sample into the well.
13. The apparatus as in
This application is related to U.S. application Ser. No. 11/696,369, filed Apr. 4, 2007, the full disclosure of which (including all references incorporated by reference therein) is incorporated by reference herein for all purposes.
This invention relates to characterizing molecular binding events for performing ligand binding assays and more particularly to systems employing ligand spots or microarrays in a closed multiwell format.
U.S. Pat. No. 6,594,011 issued Jul. 15, 2003, the entirety of which is incorporated by reference herein for all purposes, discloses an imaging apparatus and method for real time imaging ellipsometry for high throughput sensing of binding events useful in molecular interaction analysis including biotech applications. The apparatus disclosed employs the immobilization of an array of binding or capture molecules ("ligands") on a planar surface of a transparent substrate and the use of a beam of polarized light directed at the underside of the surface in a manner to achieve total internal reflection (TIR) and generate an evanescent field in the plane of the ligands. The ligands are exposed to a biological sample and analytes in the biological sample bind to different patterns of the immobilized ligands in a manner to change the polarization at locations in the array at which binding occurs. An image of the array is compared with a stored image of the initial light polarization shifts to determine the location and magnitude of binding events within the array, thus identifying and quantitating the analytes present in the biological sample.
The apparatus for implementing the foregoing technique typically employs a prism or gratings to achieve the requisite TIR generated evanescent field, the prism being the most practical implementation.
TIR imaging ellipsometry works well for fields of view up to 1-2 cm2, which permits real time imaging of tens of thousands of binding events simultaneously. However, there is a need to be able to image or scan areas which are much larger, such as 128 mm�86 mm (e.g. the area of a 384 well or a 96 well multiwell plate), to permit lower costs per test and for multiple tests per patient for large numbers of patients simultaneously, which is increasingly a requirement for clinical diagnostics and personalized medicine. Obviating the need for a prism simplifies both the instrument and disposable multiwell plate.
Also, it is well known in the art to label a hybridized target or probe by, for example, adding a molecule, either being conjugated to, bound to, or associated with the target. Labels include reporter molecules that can be detected directly by virtue of generating a signal. Examples include but are not limited to fluorophores, dyes, chemiluminescent probes, radioactive atoms or molecules, magnetic particles and quantum dots. The detection of labeled target molecules in a binding assay may employ total internal reflection as a way of exciting fluorophores, for example, which could then be detected from above or below the assay surface. There are examples in the literature which demonstrate the benefits of detecting fluorophore emission at angles close to the critical angle (see e.g., http://www.olympusmicro.com/primer/techniques/fluorescence/tirf/tirfintro.html) in such a total internal reflection configuration. The reason for this has to do with the anisotropic fluorescence emission intensity at a reflection interface between higher and lower index materials. In these other labeled examples, the light need not be polarized.
The present invention provides for total internal reflection ellipsometry without the use of prisms or gratings in a multiwell plate, thereby allowing for cost effective solutions for scaling the measurement in a way previously unimagined. If the dimensions of posts are chosen so that a beam of light can be introduced through the posts in a manner to achieve total internal reflection from the side walls of the posts, ligands immobilized on the exterior face of each post will be in an evanescent field which enables binding events between ligands and analytes in a biological sample in the well to be imaged simultaneously. In one application, the plurality of ligands form an array within each well where there are multiple posts per ligand spot. However, for larger configurations, it is possible to have arrays of ligands printed on the side walls of each post. The resulting image data is compared to a stored image of the polarization phase distributions in the beam obtained prior to the exposure of the ligand array to a sample in order to analyze the binding from the analytes in the samples.
The provision of solid transparent posts in the wells of a multiwell plate not only permits simultaneous imaging of binding events without the necessity of a tag (e.g., fluorescent, chemiluminescent, radioactive or otherwise) but also enables the placement of an array of separate ligands for each well of a plate. This configuration allows for simultaneous multiplexed assays for a number of patients for clinical diagnostics applications in one example. For rectangular posts with four walls, one embodiment includes the immobilization of a single ligand to each wall making four ligands per post. With, for example, 1000 posts in every well, for a ninety-six well plate, 384,000 individual ligand/analyte interactions can be measured in a single disposable. This represents a substantial increase in the number of possible multiplexed assays provided along with a significant reduction in the cost per assay. The formation of ligand arrays on upright walls of posts and the use of imaging ellipsometry to image binding events at the arrays thus represents a significant advance in the art.
The present invention also reduces a characteristic problem in evanescent field detection technologies, which is the problem of sediment from a sample falling down onto the detection area during measurement. Applications involving open well plates require that the plates be oriented with the open end upward. Having ligands at the bottom of open wells oriented in this manner exacerbates the sediment problem but this problem can be overcome with an array of ligands on the side wall as disclosed in the present invention.
In one embodiment, a transparent disposable multiwell plate has a pattern of wells, identical to the pattern commonly used in commercial multiwell plates. But in accordance with the present invention, each well includes a plurality of solid transparent posts each of a height equal to the height of the wells in one example. The wells and posts may be formed as a single part out of glass or plastic such that the height of the posts and the wells defines the thickness of the sample region which is enclosed after the bonding with a second plate. An array of ligand patterns is formed on the planar walls of the posts so that the reflected light from each post provides information regarding the interaction of analytes in solution to a plurality of different ligands.
In a second embodiment, microarrays are formed on the exterior surfaces of the posts, conveniently by printing, as shown in
The lateral dimensions (x and y) and the height of each post herein are chosen to allow a beam of light to be directed to a post (e.g., into the bottom of each post) in a manner to achieve total internal reflection (TIR) from the (interior) face of at least one side wall of a post and to produce an evanescent field in the plane of the immobilized ligands on the exterior face of that wall.
The microarrays conveniently comprise different ligands immobilized in rows and columns such that the individual ligands are associated with position information useful to identify the positions in the array at which binding events occur. The invention herein takes advantage of such arrays which can be printed within each well.
Ligands on a selected post may be accessed by a collimated, polarized beam individually, or all the like-facing arrays on all posts in, for example, rows of wells may be accessed simultaneously. Also, ligands on posts may be accessed sequentially by reorienting the disposable with respect to the light source and imaging system (or vice versa) or by scanning individual spots using a laser.
Also, in one embodiment, the posts are coated with a metallic film and the angle of the light source is varied to produce surface plasmon resonance at the SPR angle.
The scope of the invention is defined by the claims, which are incorporated into this section by reference. A more complete understanding of embodiments of the present invention will be afforded to those skilled in the art, as well as a realization of additional advantages thereof, by a consideration of the following detailed description of one or more embodiments. Reference will be made to the appended sheets of drawings that will first be described briefly.
Embodiments of the present invention and their advantages are best understood by referring to the detailed description that follows. It should be appreciated that like reference numerals are used to identify like elements illustrated in one or more of the figures. It should also be appreciated that the figures may not be necessarily drawn to scale.
The present invention provides an advantageous apparatus and method for performing ligand binding assays using microarrays in a multiwell plate format. Prior to describing embodiments of the present invention in detail, the following definitions are provided for use throughout the present document.
Microwell Plate: A flat plate with multiple "wells" used as small test tubes. The microplate has become a standard tool in analytical research and clinical diagnostic testing laboratories with 6, 24, 96, 384 or even 1536 sample wells arranged in a 2:3 rectangular matrix in one example.
Ligand: Any molecule that binds to another, in normal usage a soluble molecule such as a hormone or biological molecule that binds to a binding partner or capture molecule. The decision as to which is the ligand and which is the capture molecule is often arbitrary. In the sense of this invention, the ligand refers to that binding element attached to a planar surface and which binds to an analyte molecule in a biological sample.
Total Internal Reflection (TIR): An optical phenomenon that occurs when light strikes a medium boundary at a steep angle. If the refractive index is lower on the other side of the boundary (i.e., the side that doesn't directly receive the light) no light can pass through and effectively all of the light is reflected. The critical angle is the angle of incidence where total internal reflection begins and continues up to angles of incidence of 90 degrees.
Ellipsometry: A very sensitive optical measurement technique providing unequalled capabilities for thin film analysis utilizing the change of polarization of light which is reflected off a sample or transmitted through a sample.
Surface Plasmon Resonance (SPR): The excitation of surface plasmons by light is denoted as a surface plasmon resonance for planar surfaces. Plasmons are collective oscillations of large numbers of electrons in matter, mostly in metals.
Arrays: Ligands affixed to a surface at separate localized regions called spots in an ordered manner thus forming a microscopic pattern where ligand identity is determined by the location (or "address") of that particular spot.
Binding Protein (Ligand) Assay: A test that uses the binding of proteins (e.g., antibodies) to other ligands (e.g., antigens) to identify and measure the concentration of certain biological substances in blood, urine or other body components. Ligand assays may be used to diagnose disease, drug or vitamin levels, response to therapy or other information of biological relevance. Also, test results can provide information about a disease that may help in planning treatment (for example, when estrogen receptors are measured in breast cancer).
For a 96-well plate as an example, a well has dimensions of 7 mm by 7 mm for square wells or a diameter between 6 and 7 mm for circular wells, and each post is solid and has a height between about 25 μm and about 100 μm to be substantially equal to the depth of the shallow wells. The four well section of plate 10 which is shown in
The embodiment as shown in
In another embodiment of the type shown in
After the ligand spots dry, plate 30 is rinsed thoroughly to remove excess material and a cover plate 40 of
It is to be understood that the use of posts in a well, in accordance with the principles of this invention is practical whether a plurality of like ligands or an array of ligand patterns are immobilized on post sidewalls (and tops) or whether the posts are smaller or larger than the dimensions of the ligand spots.
In one example, in order to image binding events, the ligand array is accessed by collimated, polarized light directed from below plate 70 at a post wall in a manner to obtain TIR at the wall. This is to be contrasted with previous arrangements typical in the art operative to achieve TIR at the horizontal bottom surface of the plane on which ligand arrays are immobilized.
In order to achieve total internal reflection (TIR) for imaging the pattern of binding events on a wall of a post in the array of wells of
In one embodiment compatible with current multiwell plate technology, individual wells of the plate are spaced according to Ansi/SBS standards (e.g., 4.5 mm center to center for 384 well plates and 9 mm center to center for 96 well plates). The spacing of the wells is true to standard. But, in accordance with the principles of this invention, the addition of posts to a well and the dimensions of individual posts are important. In fact, there is a minimum post thickness in at least one dimension to allow total internal reflection to occur at precisely the critical angle from the side wall in such a way that the entire surface may be illuminated. This minimum post thickness depends on the index of refraction of the plate, the index of refraction of the material inside the wells during a measurement, and the height of the posts. The formula for post thickness in the direction of light propagation is the following:
The manner in which the arrays are printed is well understood and described in the literature on topics dealing with technological aspects of DNA and protein microarrays. Contact and non-contact printing techniques for ligands are applicable. Ligands may be immobilized on all the (exterior) walls of a post or several posts with a small drop of solution larger than the dimensions of the individual posts. However, when plates 10 and 30 are joined, the array on the top of a post becomes inaccessible and does not affect signals generated in the light reflected from a side wall since the refractive index of the protein is close to the curing adhesive which binds the two parts together. Furthermore, optical thin films may be provided on the sidewalls to amplify signals within the evanescent field region on those surfaces.
The interrogating beam from source 102 may be directed at the (ligand) wall of a single post or simultaneously at the (ligand) walls of a plurality of posts which are in the cross section of the collimated beam. Moreover, the beam may be redirected by conventional implementations (i.e., small scanning mirrors) to access all the wells of each of a sequence of rows and columns of
In accordance with another embodiment of the present invention, the apparatus of
The disposable multiwell plate in accordance with the present invention has a number of differences and benefits over prior uses of imaging ellipsometry.
In the case of the imaging system 104, the camera itself need not be a sophisticated, high resolution CCD camera if, indeed, relatively few ligands are immobilized on each ligand wall as would be the case for most current diagnostic use. In such cases on the order of a few to tens of ligands need be immobilized for diagnostic purposes. This lower demand for content translates to less expensive cameras and more cost-effective systems configured for doctor offices and clinical laboratories performing diagnostic testing. For other purposes such as biological research in metabolic or cancer pathways, drug target interactions in drug discovery, and biomarker profiling during clinical development of new therapeutics, higher content of ligands in arrays (100's to 1000's) may be required.
In one embodiment, the invention has been described in terms of total internal reflection ellipsometry employing polarized light and sensing localized changes in the phase of s- and p-polarized light. In another embodiment, SPR has been utilized. In yet another embodiment, the entire system may be used for sensing labeled targets as well by placing a filter in front of the detector to block the reflected light and transmitting only the light emitted from the fluorophore as is done with commercial microarray scanning systems. It is intended that the invention herein applies to labeled as well as unlabeled systems. It will be further apparent that various existing analytical techniques and future analytical techniques in conjunction with the side wall concept are within the scope of the present invention.
What has been described is considered merely illustrative of the invention herein and it is apparent that various modifications thereof may be devised by those skilled in the art still without departing from the spirit and scope of the invention as encompassed by the following claims. For example, a post need not be rectangular in shape. It need only have at least one planar wall on which ligands are immobilized. If more than one wall of a post is planar, ligands can be immobilized on each planar wall and the plate can be reoriented with respect to the light source and imaging system (or vice versa in which the light source and imaging system can be reoriented with respect to the plate) to produce the requisite TIR operation. Also, although simultaneous access of multiple ligand arrays as well as scanning from one ligand array to another have been described herein, it is contemplated that the individual spots of an array can be scanned in the manner described herein, for example, by a laser. Consistent with conventional multiwell plate usage, the wells may be left open or the well array may be sealed as described herein.
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Arenas del Mar
Manuel Antonio, Costa Rica
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Arenas del Mar is a beach and nature resort located next to Manuel Antonio National Park on the Pacific coast of Costa Rica. This Manuel Antonio resort caters to tourists that are looking for more than just another high-end resort experience. Arenas del Mar Hotel offers a luxurious lodging experience in harmony with Costa Rica's nature in a spectacular beach and rainforest setting. Guest rooms, apartments and suites offer spectacular ocean views, upscale dining in two hotel restaurants with a focus on fresh local products and personalized service that exceeds guest expectations.
Arenas del Mar Beach and Nature Resort has 38 guest rooms including ocean view apartments with two bedrooms and three full bathrooms. All guest rooms offer spectacular views of the Manuel Antonio National Park, the Pacific Ocean and the tropical rainforest. The great advantage of the guest rooms at Arenas Del Mar is that they are located on secluded beach.
Arenas Del Mar features two gourmet restaurants famous for their use of seasonal ingredients and local foods featuring Costa Rican Cuisine with an international flavor. The more formal restaurant El Mirador hangs over the Espadilla Beach with its white sand beaches and the sparkling Pacific Ocean and overlooks the spectacular Manuel Antonio National Park.
Arenas del Mar is the perfect base to explore the area around Manuel Antonio and Quepos on the Central Pacific coast of Costa Rica. Tours and activities offered in the area include whitewater rafting, mangrove kayak and boat tours, guided rainforest hikes, ocean kayaking, catch and release sport fishing, Canopy Tours and horseback riding excursions.
Sustainability is a key component of the concept at all levels of the operation and the Certification for Sustainable Tourism-a program designed by the Costa Rican Tourism ministry-serves as a guide for the building and operation of the hotel. Arenas del Mar sets out to minimize the impact on the surrounding nature, while maximizing the positive economic impact on the local community through employment.
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- Complementary breakfast included in the rate
- Children under 6 years old are free of charge, sharing the room with an adult in room or suite, and 4 adults in the apartment. Full Breakfast a la carte at El Mirador Restaurant is included.
- Children 6 years and older pay full rate for room and meals.
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High & Low Season (Jan 6- Dec 14): If there is a "no-show" 100% of the total of the reservation will be charged
If a reservation is cancelled 22 days or less prior to the arrival date, 100% of the reservation will be charged.
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If a reservation is cancelled 15 days or less prior to the arrival date, 100% of the reservation will be charged.
If a reservation is cancelled 30 days or less prior to the arrival date, 50% of the reservation will be charged

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text: The The Legend of Zelda: Oracle of Ages wiki last edited by admachina on 04/17/13 07:33AM View full history OverviewLink and Impa in the forestThe Legend of Zelda: Oracle of Ages is a Game Boy Color game released simultaneously with The Legend of Zelda: Oracle of Seasons and follows Link's adventures through the land of Labrynna. The game is published by Nintendo and developed by Flagship. The two Oracle games feature unique connection options, allowing players to collect treasure and objects using a link-up or password mode.The developers intended initially to make three games, all of them representing one part of the Triforce. Upon realizing that this system would be too difficult they canceled the third game and decided only to work on Oracle of Ages and Oracle of Seasons.Oracle of Ages and Oracle of Seasons release dates were very close to that of the Game Boy Advance. The developers were indecisive as to whether or not they should add Game Boy Advance functionality to the game, because doing so might push back the release date and result in the game coming out after the Game Boy Advance launched. Luckily, the Game Boy Advance was delayed, giving them enough time to add the functionality and the games still came out one month before the Game Boy Advance.GameplayAn example of the side-scrolling gameplayOracle of Ages is a traditional Zelda game. Link explores the overworld, gathering clues and occasionally, items. After traveling about, Link makes it into various dungeons (eight dungeons are in this game, referred to as levels) and collects an item within each one that is normally used to go about the dungeon and eventually beat the dungeon's boss battle.A variety of areas in the game are not in the traditional Zelda style. Instead, they take a style more familiar with the Mario games. In these side-scrolling areas, Link can still use all of his items as usual.Introduced in this game are Gasha Nuts and Magic Rings. Gasha Nuts can be planted in certain areas around the overworld. After they grow, the seed on the tree can be opened for an item, normally a Magic Ring. Magic Rings can be appraised and then worn, for various effects, from transforming Link into an Octorok, to upping defense.The game makes heavy use of the Harp of Ages which opens up time portals to go between the past and the present. In the late game there are additional "Tunes" which don't require the usage of a portal to travel through time, allowing players to travel at will between the two times. There are many things that are changed between the two times. One example is that the Black Tower is not finished in the past, but it is finished in the future. Some other buildings also have slightly different layouts depending on the era.After finishing Oracle of Ages, passwords can be gathered in Oracle of Seasons which can then be told to various NPCs (non-playable characters) in the overworld. After telling the various NPCs the password, an item is presented to Link. Items vary from the Master Sword to a Piece of Heart. After collecting the item, the password given to Link by the character can then be used in Oracle of Seasons.StoryThe game starts with Link dropped into Labrynna. After walking around he finds a woman flanked on all sides by monsters. Upon Link's arrival, the monsters run away and the woman (who turns out to be Impa) asks Link to help her find a singer in the forest. The two find the singer, who is Nayru, the Oracle of Ages, singing and playing her harp for the forest creatures. The demon sorceress Veran emerges from Impa and possesses Nayru's body which causes all time in Labrynna to go into chaos. Impa gives Link a sword and tells him to go see the Maku Tree, who tells Link that he must get the eight Essences of Time to rescue Nayru.After collecting the essences, Link faces Veran. The game doesn't end after the credits roll, unless Oracle of Ages is being played as a linked game. A cutscene is shown at the end of the game in which Koume and Kotake (better known as Twinrova) plot their revival of Ganon. A code is shown at the end of the game as well, and if typed into Oracle of Seasons when starting a file, the real ending of both games will be shown.CharactersCharacter DescriptionImageLinkLink is the main protagonist of every official Legend of Zelda game (except for a few games on the CD-i, although those games are not technically considered part of the Legend of Zelda franchise). In Oracle of Ages, Link is sent to Labrynna by the Triforce, and then tricked by the evil Veran into opening seals that protect Nayru. Link then retrieves the Essences of Time and uses them to defeat Veran.NayruNayru is the Goddess of Wisdom, and one of the three goddesses who created Hyrule. In Oracle of Ages, Nayru is a singer. Her voice brings peace to all who hear it; which attracts many visitors. She is possessed by the evil Veran at the beginning of the game, and upon getting free, Veran possesses Queen Ambi instead. Link and her friend Ralph are both attempting to save Nayru from Veran, and Link manages to do so by using the Essences of Time.VeranVeran is the main antagonist of Oracle of Ages (although Twinrova is the main antagonist of the two games). Veran is the Sorceress of Shadows, and she uses her unique abilities to possess several people in the game (Impa, Nayru, and Queen Ambi). She first appears when she possesses Impa, and harmlessly requests that Link escorts her to Nayru. She tricks him into taking her to Nayru, and promptly possesses her. Link eventually tracks down the possessed Nayru, and manages to defeat her.Veran escapes, however, and proceeds to possess Queen Ambi. Under this new influence, Queen Ambi begins to build the Black Tower. Upon completion of the tower, she flees to the top floor. When Link manages to make his way up to the final battle, he frees Queen Ambi from the control of Veran. Veran finally transforms into her final form and flies around the room attacking Link. Upon her defeat, the Black Tower begins to collapse, but Link and Veran fall to the base of a tower. This is where their final showdown takes place, and Veran takes on many forms. Link manages to destroy them all, but not before she is able to light the Flame of Sorrow, one of the three flames that is needed to resurrect Ganon.RalphRalph is a friend from Nayru's childhood, and he assists in guarding her. In the beginning of the game, he distrusts Link, but as the game progresses, he begins to like Link more and more. He is a descendant of Queen Ambi, although he never mentions the throne. Ralph appears consistently throughout the game to provide his advice and opinions. He is resourceful and knowledgeable, making him an essential part of Link's success.Queen AmbiQueen Ambi (despite being under the influence of Veran for a large portion of the game) is the queen of Labrynna. Her one true love was lost at sea, so she began to build a large tower so that he could see it and return to her. If Oracle of Seasons is linked to Oracle of Ages, Queen Ambi is able to meet her lover.ImpaImpa is the protector and maid of Princess Zelda. She was originally sent to bring Nayru and Din to Hyrule so that the lands of Labrynna and Holodrum would be safe, but she fails at this quest when she is possessed by Veran. Link must then save these lands. In the beginning of the game, she is under attack by Octoroks, similar to the original The Legend of Zelda. After Veran leaves Impa, Impa gives Link a wooden sword (if it is a linked game, then she will give the player a wooden shield).


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Students and volunteer library and information science professionals helps maintain the ipl2's collections. http://www.ipl.org/div/kidspace/
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October 7, 2013
Col. Don Davis, commanding officer of Marine Corps Logistics Base-Albany, meets with a group of civilian employees on the base Monday. Many of the base's employees are back at work, but will not get paid until a resolution by Congress is made. (Staff Photo: Jennifer Parks)
Stories this photo appears in:
MCLB workers back on base
Civilian employees aboard the Marine Corps Logistics Base-Albany installation have all been recalled and internal base functions have resumed as normal as of Monday.
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Smith has joined the Rams on a season-long loan from Tottenham and the 22-year-old right-back sees no reason why Nigel Clough's team can not finish in the top sixcome May.
He told the club's official website: "I think moving here for a season will be brilliant for my career and from what I have seen and know about the team, we can challenge for the play-offs.
"I'm really excited by this opportunity to come to a massive club and play first-team football. I can't wait to get started.
"As soon as my agent told me that Derby wanted me, it was a simple decision. I knew I was going out on loan anyway this season but it was great news to hear Derby wanted me."
"There was interest from other clubs but coming here was my first choice."


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South Devon College's staff and students land awards
Posted: December 04, 2012|
STAFF and students are celebrating winning not one but two awards at South Devon College.
The college has been awarded two prestigious Association of Colleges Beacon Awards for Sport in the Curriculum and for the Effective Use of Technology.
There were more than 20 applications for the sport award for the approach to provide high quality teaching and learning through a distinctive, inclusive and comprehensive sport curriculum that has inspired the local community through sport.
The college has established extensive partnerships with local and national agencies which ensure students have access to a wide range of activities to enhance their learning experience.
The approach has resulted in students developing high levels of skills, excellent success rates and outstanding progression to higher education or employment.
South Devon College won its second award for the implementation of a college-wide tutorial system, known as Learner Engagement and Progression, which has allowed staff to access information about every learner and maintain regular updates of how they're doing so they can be properly supported throughout their time at college.
The college has also established the use of Independent Learning Officers who support learners at level three in the development of research and study skills. This approach has freed up the subject staff to return to their specialism.
These two approaches combined have led to a significant improvement in success rates for the students as well as delivering significant cost savings for the college.
South Devon College principal Stephen Criddle said: "I am extremely proud of the college's success. These national accolades are a great reflection of the wonderful work and staff at the college."
The college will host a local celebration event in the New Year and will be presented with its awards at a national presentation ceremony in London in February.


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Originally Posted by Alter2Ego
ALTER2EGO -to- ANY8123:
God tells us in his inspired Word, the Bible, that death is the opposite of life. The terminology "could be" is nothing more than personal opinion aka speculations. But since that seems to work for you, go for it.
So is the Bible, it is no different than any other written book it was transcribed by human interpretations of events of history. The bible didn't exist before humans, it wasn't discovered by humans centuries after creation, it was derived from events that transcended human interpretation at the time of said event. So the terminology "bible" could also be interpreted as opinion. Nothing is absolute when it is open to interpretation.
Originally Posted by Alter2Ego
ALTER2EGO -to- ANY8123:
I don't recall quoting any scriptures dealing with death in my previous post.
But you did. You claimed "According to the Bible, the dead are unconscious and know absolutely nothing." which is a reference to Eclessiastes 9:4-6 which is also open to interpretation and not an absolute. Most theologists would say that scripture represents the separation of soul and body, we leave behind what we were for what we are to become, again a human interpretation.
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Tuesday, February 12, 2013
Letting go
How do you know when to let go, or when to step in with your child?
This is a complicated issue. I mean, as a mom you start out doing everything for your child.
Everything. And somewhere along the line they want to do it themselves, usually around age two, which is when the word No appears in their vocabulary as well.
I have made breakfast, lunch and dinner for the last 11 years, for one or more of the kids every day. When is it time for them to start making their own? And not just are they ready, but am I?
I hate putting laundry away, and it took me a long time to let go and be okay with them putting their own laundry away.
In my own defense it had nothing to do with them putting it away, so much as I had to be okay with HOW they put it away, which is usually shoved in a drawer...
And if they are doing things for themselves, like making lunch but then leaving the kitchen a total disaster, isnt it easier for me to just make lunch?
Otherwise, I have to get them to come back in and clean up the mess too, which is more work.
By nature I am a fixer and caretaker, so I want to take care of everything.
I know I am not helping them learn to be self-sufficient by trying to do everything for them. I just wish that it was a bit simpler to know when they are ready to do something.
Even a turkey has a turkey timer, so you know when its ready, but I am just supposed to just guess as to when my child is old enough to stay home alone for an hour?
That makes no sense!
And when a kid at school is mean to your child, when do you step in, and when do you let them work things out?
I always want to step in and work things out for them.
Will that ever change or for the rest of my life am I going to want to beat up every mean person that my kids run into?
Learning to negotiate and work through differences is a major life skill.
I want them to learn that, but in order to learn theyll have to get hurt.
Which I dont like.
Plus, there are four of them and I would be really busy following them around being mad and working things out for them if I dont let them learn on their own.
Perhaps I am over-thinking this.
Really, this whole train of thought started because Dylan wanted to put cream cheese on his bagel himself. He is eleven, and its just cream cheese, but he still needs me, right!?
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digitalhealthsummit via Flickr
September 2, 2013
We've used the internet to connect computers. Thd next step could be using it to connect our brains. Researchers at the University of Washington have done just that in what they say is the first ever brain-to-brain interface.
Earlier this month researchers sent a signal over the internet from one person's brain to remotely control the hand movement of another individual, making his index finger move on a keyboard.
This kind of one-way, brain-to-brain communication represents a big step forward in brain-computer interfacing, but the researchers want to take it even further and create two-way communication.
Professor cognitive sciences at the University of Rochester, Duje Tadin (Dooyeh tah-deen) says that's a long way off.
But, he says if technology advances to allow brains to communicate directly, some enormous ethical issues would be raised.
"If you can control another being from long distance, then you really have an issue of is this person acting on his own behalf, or is he being controlled by someone else. And this really sounds like science fiction."
At first blush, this breakthrough brings to mind all sorts of science fiction scenarios.
It may take years, but eventually researchers say their breakthrough could allow a person with disabilities to communicate their needs, or it could be used by someone on the ground to help land a plane if the pilot becomes incapacitated.
Tadin says those kinds of applications would be very useful in many fields, but it requires the decoding of complex brain processes, and that's a tall order.
"If you're a really good pilot there's going to be many different brain systems all working together at the same time, so if you want to turn a passenger into a really good pilot, you'll have to figure out how to activate all those brain systems."
The University of Washington researchers responsible for the breakthrough say the next step is to transfer more complex information from one brain to another.


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(Memphis) A Memphis man is facing two counts of aggravated child abuse in the death of his three-year-old son.
Landon Stokes died yesterday at Le Bonheur from injuries he received to his head and body.
Jamie Jones told police he sent Landon to his car outside their apartment in the 4100 block of Rainbranch and when he came outside he found the toddler at the bottom of some stairs.
One neighbor, who didn't want be identified, said he never heard any commotion outside his door.
"If the boy fell down these steps there would be blood traces down there from him falling down here. There is just no way," said the neighbor.
Police aren't buying the father's story either.
Doctors say when Jones brought Landon to Le Bonheur the boy's injuries were several hours old and not consistent with a fall.
Neighbors are shocked something like this happened.
"It's horrible. I can't think of nobody doing anything like that to their baby," said Kesha Dooley.
Jones is being held in the Shelby County Jail on $750,000 bond.
For more news updates from the field follow me on twitter


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Richard III dig: 'R' marks the spot where skeleton found in Leicester car park
Posted: February 03, 2013|
The latest astonishing twist in the extraordinary story of the search for Richard III can exclusively be revealed by the Leicester Mercury today.
The spot where a skeleton was found in August was next to a car parking space marked with an "R".
The letter was noticed by academics at the University of Leicester at the start of the dig - and it was coincidentally captured in a photograph of Greyfriars car park taken by the Leicester Mercury before the excavation began.
The picture, shown above, reveals a faded "R".
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LIVE UPDATES: Richard III DNA results announced - Leicester University reveals identity of human remains found in car park
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We have marked the spot, just a few feet away, where archaeologists discovered human remains which could turn out to be those of the king.
An eagerly-awaited press conference on Monday morning will reveal the results of tests which have been carried out on the remains by university experts.
These include comparing DNA extracted from the bones with that of descendants of Richard.
Archaeologists, geneticists, genealogists and historians will present their evidence to the world's media before the project's lead archaeologist, Richard Buckley, announces the final verdict.
Nobody knows exactly why the letter had been painted in this spot. It is thought it might have been to indicate a reserved parking space.
Mr Buckley said the team had never taken the mysterious sign seriously, but admitted it was "spooky".
"I used various sources, such as old maps and accounts from historical authors to draw up the trenches," he said.
"I admit, when we started the dig, I was sceptical – I didn't think we had much chance of finding him.
"I was more interested in finding the friary where he was supposed to have been buried.
"Philippa Langley, from the Richard III Society, was present when we were deciding where to dig, and kept telling us that R marked the spot.
"We had a joke about it, but we never for one moment thought anything would be buried underneath it.
"This is a serious academic project and the implications – if it turns out to be Richard III – are huge.
"But I think the mysterious R is quite nice and gives the whole thing a bit of levity, which we could probably do with every now and then."
The car park is owned by Leicester City Council.
The Mercury asked the council if it knew why the letter had been painted on the Tarmac, but staff at the council were as perplexed about it as the University of Leicester dig team.
Mick Bowers, principal property review officer at the council, said: "I was based at Greyfriars for many years and remember the R being there for most of them.
"It was something of a standing joke before the dig started that R obviously marked the spot as it seemed a strange thing to spray on a car park.
"The best we can come up with is that maybe it signified a reserved parking spot for someone based at St Martin's.
"But that doesn't quite ring true, because it wasn't painted in a parking bay, it was more towards the centre of the car park.
"I've asked other people who have been around for a while, including the car park attendants, to see if they can shed any light on it but unfortunately not."
Archaeologist and Greyfriars site manager Mathew Morris, who found the skeleton, said: "We don't know how the R got there and, to be honest, I missed it at first.
"It's only when someone came to us with the photo and said 'have you seen this?' that I thought 'wow, that's a bit weird'."
LIVE COVERAGE AS THE FINAL VERDICT IS ANNOUNCED AT 10AM ON MONDAYFollow the all-important press conference as it happens, with live reports and pictures,on This Is Leicestershire - here.Follow us and join in the debate on Twitter via @thisisleics using #richardIIIReporters Pete Warzynski and Laura Elvin will be tweeting live from the press conference. Follow them at @BigDaddyPete and @egoelvin


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Fenphedrine Review
What You Should Know
Click Here To See Our Highest Rated Review
Fenphedrine is a fat burner and appetite suppressant designed to help stimulate weight loss. We will take a closer look at the product to determine if it is worth the investment for those who are looking to lose a few pounds.
List Of Ingredients
Fenphedrine contains the following ingredients: Infinergy dicaffeine malate, HOPS, Chocamine, Razberi-k, Ginger 20% gingerols, Phenylethylamine(PEA), Evodiamine 98%, Sclareolide, and 1,3 Dimethylamylamine(DMAA).
Product Features
Fenphedrine features a unique blend of ingredients known to help burn fat and suppress the appetite to help you lose weight. The dicaffeine malate is a patented blend designed to buffer out the negative side effects from the stimulant while still giving you the benefits from the caffeine. HOPS, otherwise known as humulus lupus, is a relaxant for the body and mind to combat stress that triggers the hormones that hold onto fat in the body. Chocamine comes from chocolate and offers numerous health benefits including: more fat burning, appetite suppression, and increased energy levels. Razberi-k is a natural fat burner. , Ginger 20% gingerols helps to soothe the stomach and take care of the digestive system. Phenylethylamine(PEA) is commonly known as the "love drug" in chocolate, and is used as an appetite suppressant. Evodiamine 98% is a natural fat burner. Sclareolide is another natural fat burner. 1,3 Dimethylamylamine(DMAA) works like adrenaline does on the body, which triggers the body to burn more fat.
- Fenphedrine contains many proven ingredients to help burn fat and suppress the appetite.
- Fenphedrine has several fat burners, but we don't know if there is enough of the ingredients in the formula to produce real results.
- There are a couple of stimulants in the formula which may cause many negative side effects including: sleeplessness, jitters, irritability, and headaches.
- At $69.99 per bottle, this is a costly supplement for most people to take on a regular basis.
- We cannot confirm a money back guarantee.
- There is not a free trial product offer.
Fenphedrine seems like it could be a viable product, if we only knew how much of the ingredients were actually in the formula. Without being able to tell the ingredient amounts, we have no way to know whether or not there is enough to be able to produce effective results. Since we cannot tell, we do not recommend the product to our customers. Instead, find a proven supplement with proven ingredient amounts, and use it with a balanced diet and exercise program.


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The original Dissidia met a mixed response from fans due to its rather unique combat system. Instead of copying the model of other fighters -- Nintendo's Smash Bros., or Capcom's Vs. series -- Square Enix crafted a battle system inspired by Advent Children, but still rooted in RPG mechanics. If that doesn't make much sense to you, you are not alone.
Arguably the biggest new feature for Duodecim is the addition of an "RPG Mode" that strips down the combat experience into something that might be a bit more familiar to traditional Final Fantasy players. Unlike the traditional "Action Mode," you lose direct control over your character in this mode, relegated to selecting commands. You'll be able to switch between attacking Bravery and HP, or choosing a more defensive role. It may seem like the game is simply controlling itself, and to a certain degree, it is. However, this mode lets you understand the various mechanics that are at play in Dissidia without being overwhelmed.
If you've already mastered the nuances of Dissidia's combat system, you'll probably skip this new feature. Instead, there are new mechanics to master. You now can assign an Assist character, and as in Marvel vs. Capcom and Naruto Ultimate Ninja, you'll be able to summon an ally with a quick button press. Depending on your Assist, you'll be able to stop a fleeing opponent, deal more damage, or lengthen your combo. The Assist also provides the valuable fan service of hypothetically making Cloud and Sephiroth partners -- you can already hear the fangirls squealing.
The new characters also offer their own unique abilities. Lightning, for example, is able to use Paradigm Shift on herself to change her battle style on the fly. It'll be interesting to see how other additions to the roster will change the experience. There's also multiple difficulty settings, which should appease both newcomers and hardcore veterans alike.
I wasn't expecting much more than an expanded roster out of Dissidia's sequel, so I'm glad to see Square Enix make some rather significant enhancements to the experience. Final Fantasy fans that dismissed the first game will definitely want to give its sequel a second look. Duodecim will be available in Japan in 2011.
- Key specs
- Reviews • 108
- Prices
- Discussions
- Game format Optical disc, Downloadable
- Screen size 4.3 inches
- Online features Multiplayer, Voice chat, Store, Browser
- Direction control D-pad, Thumb stick (1)
- Camera External (1.3 megapixels)
- Dimensions 71.4 x 169.4 x 18.6 in
- Weight 6.67 oz
- Discontinued 2008-10-15


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Today is the 31st October, the last day of pastor appreciation month. I thought I would take a moment to give thanks to a few pastors who have helped me along the way this past twelve months. I am not sure if it is a sad fact but many of them I have never met. I have few people locally with whom I share a vocational connection with. It can get lonely at times.
- Firstly to my mentor John. John has walked with me through my thesis these past few years and helped me to work out the direction I should be taking. This is he has done despite his own health battles. John has taken time with me. I appreciate that.
- Stephen Spence, my thesis supervisor. Although he occupies an academic chair (technically I think its an office chair but I digress) Stephen is a pastor. He has been a wonderful help to me in framing my thesis. Without his guidance I would not have gotten this far.
- Clayton Blackstone. I met Clayton through Joshua who once blogged at Near Emmaus. Clayton has been to me the almost perfect model for faithful pastoral service. It helps that he too is a Petersonian. Clayton has taken time to listen and care over these past few months. Clayton has not only been a wonderful encouragement he has in many ways been my pastor. Clayton, thank you.
- My good friend David Kowalick. Coffee shops are richer for having us frequent them. It is great to have a friend who understands and walks a similar path to me. Our churches are very different and yet in many ways the same. You have also been a good friend to me these past twelve months. I love that you are so passionate about the gospel.
- A HUGE thank you to Daniel Thompson and Brian Fulthorp. Both of you have restored my faith in Pentecostal pastors and helped me to see the good in the tradition I came from. Daniel, keep on with what you are doing it is too important not to. And Brian, hang in there pal. You are right where God wants you…even if it is the desert!
- To Clay Knick. Thanks for all of the book suggestions and the conversations we have shared via Facebook. Pastors as happy and positive as you are few and far between!
- To my friend Doug. Not technically a pastor but a pastor nonetheless!
- One or two pastors have caused me great anguish this year. I am not sure they meant to but in doing so they have reminded me of what is truly important. (As an aside neither of these are local guys).
- To my elders. Thank you for being my companions. I love working as a team with you all. You look out for me and you look after my family. Words cannot convey how much I love serving you.
And finally to the greatest pastor I have never met and yet feel as if I know. To Eugene Peterson, your voice is a constant companion to me. Your works drew me into the pastoral vocation and they sustain me in many a dark time. But most of all they remind me to keep it real and faithful. . I wish that one day we would meet or at least talk by phone. If it be God's will. Thank you for writing The Pastor. I read this quote by you today and it is no wonder I resonate so well with your words, "I grew up Pentecostal. I never had a pastor. They were all leaders. They had goals and ambition…I never wanted to be a pastor."
To all pastors out there. I pray that you are encouraged in Christ. Please, continue to preach the gospel. Be ready in season and out of season. There awaits for you a crown of righteousness. May God bless you and keep you in all that you do.
Grace and Peace.


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Hammered Barrel Mug, 14 fl oz
The hammered ceramic body and black reactive glaze make this barrel-shaped coffee mug a candidate for coolest mug in the office. The desktop mug's plastic press-in lid can also swivel open and closed, making it perfect for jumping from room to room, or meeting to meeting. Holds up to 14 fl oz. Dishwasher safe. Do not microwave.
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$11.95 / 12 count
$24.95 / 63 fl oz
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$37.95 / 50 count
Reviewed by 13 customers
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It's microwavable and dishwasher safe; it has a handle and a lid for no spills. Yet it's shaped like a traditional mug instead of a top-heavy "travel" mug. I love this purchase!
Great coffee mug for the office, lid prevents spills and keeps drink hot. Nice size and really like the hammered body of mug.
Received it as a gift. Love the look and size and it fits perfectly on my mug warmer. Problem is coffee drips/ spills every time I take a sip no matter how I adjust my sip. Terrible design on the cover, which drew me in the first place.
I think this is a very masculine looking mug that any man would enjoy.
It's a very nice looking mug but it does not retain heat at all and the plastic lid was not designed very well. It's hard to drink with the lid on without having it drip all down the side. Very disappointed with these mugs!
Looks great, feels great, holds a good amount of coffee, but because of the air hole in the lid it just doesn't keep coffee hot for very long. Disappointing. When the opening to drink is closed, the air hole should close too. If Starbucks would add better insulating properties in the mug itself, and keep heat from escaping, this could be a real winner. As it currently stands, it's just average compared to other insulating mugs I have (Keurig makes an outstanding thermal mug that stays hot for hours).
Saw this quaint little item in the store, and then ordered online. It is larger than I had expected, and a bit heavy when full, but it makes a nice morning coffee nightstand item. The sip spout takes a bit of practice, but the fact that it closes all the way is great for keeping liquids hot. Terrific that it is microwaveable for refills on lazy weekend mornings.
The Hammered Barrel Mug is less than a satisfactory product. It leaks if you have the opening fully open. It is actually probably better without the lid on it. So, on the whole, it is moderately attractive but not a very good mug.
Purchased this as part of a set with a coffee press and coffee for Father's Day ... so thrilled with it! It's exactly as it's pictured ~ and everything I thought it would be as a Starbucks mug. Perfect size, hammered flat finish, easy to hold and even comes with a lid. So handsome!
I found the mug to be very substantial. Pretty design and looks great. But have the same problem as everyone else with the lid. Doesn't fit very well.
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*image shown is 5 x 8 or larger rug
*smaller sizes will not show complete pattern, will be scaled down to fit.
Name: Urban Bungalow UB14 Khalid Area Rugs
Manufacturer: Jaipur
Collection: Urban Bungalow
Material: Wool
Construction: Flat Weave
Colors: Ivory/Beige , Yellow/Golds
Origin: India
Description: A range of beautifully designed flatweaves in a stunning color palette. Hand woven from 100% wool each rug has its own personality and is versatile and easy to use. (Yellow Urban Bungalow UB14 Khalid Flat Weave Wool Rug)


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Topamax (Topiramate) is used as monotherapy or adjunctive
therapy for partial onset seizures and primary generalized tonic-clonic
seizures; adjunctive therapy for seizures associated with Lennox-Gastaut syndrome; prophylaxis of migraine headache.
Some doctors prescribe Topamax as a mood stabilizer for people with bipolar disorder (manic depression).
The mix of phentermine and topiramate produced significant weight loss (up to 10%) in obese people over the course of
one year, according to researchers at Duke University Medical Center15.
Phentermine/topiramate mix appears to provide additional health benefits, including significant improvements in blood
pressure, blood sugar measurements (hemoglobin A1C), cholesterol, and triglycerides.
Phentermine/topiramate combo is being developed under the trade name Qnexa by Vivus, a California pharmaceutical company.
In October 2010, the FDA advisory committee voted 10-6 against approval of the phentermine-topiramate combination for weight loss. The panel members and the agency agreed that the combination was effective. The FDA turned down Qnexa's application due to safety concerns: increase in heart rate (an average of about one extra beat per minute), adverse psychological events, impaired memory and concentration, and teratogenicity.
In July 2012, the FDA approved Qsymia (phentermine and topiramate extended-release) as an adjunct to diet and exercise in obese patients with a body mass index (BMI) of 30 or more or overweight patients with a BMI of 27 who have at least one weight-related comorbidity, such as hypertension, type 2 diabetes, or dyslipidemia.
Topamax for Headaches
The study has shown that Topamax may decrease the number of the headache
days per week, significantly decrease the headache severity, reduce
the headache hours per day and weekly analgesic consumption. These benefits continued for an average follow-up of 8+/-4
months. The average effective dose was 100 mg/day. Slowly increasing topiramate
at moderate increments resulted in high tolerability 2.
Topamax is also safe, well tolerated, and highly effective at low doses for chronic daily headaches in children
Topamax for Weight loss
The evidence of a strong weight-reducing potential of Topamax
is indisputable and clinically significant. Topamax often results in a significant weight reduction which appears
to be one of the most attractive consequences of the therapy.
For overweight people, this may be a good thing. However, for some people
weight loss may be a highly undesirable (for example, for growing children).
Topamax is not FDA approved for weight loss, but many clinical
studies have demonstrated it's weight reducing potential.
In long-term clinical study at 60 weeks, patients in the placebo
group lost 1.7% of their baseline weight, while patients
in the Topamax 96, 192, and 256 mg/day groups lost 7.0,
9.1, and 9.7%, respectively. Weight loss >/=5% of baseline weight
was achieved by 18% of subjects in the placebo arm vs 54, 61,
and 67% of patients receiving topiramate 96, 192, and 256 mg/day,
respectively. Weight loss >/=10% was achieved by 6 vs 29, 40,
and 44%, respectively. Weight loss was accompanied by significant
improvements in blood pressure and glucose and insulin. The most
common adverse events more frequently observed in topiramate-treated
patients occurred mostly during the titration phase and were related
to the central or peripheral nervous system and included paresthesia,
difficulty with concentration and attention, difficulty
with memory, language problems, nervousness, and psychomotor slowing.
It is proposed that Topamax contributes to drops in body weight via energy expenditure elevations; reduced
caloric intake and the activity of salivary enzymes; reduction in leptin and corticosteroid concentrations;
and reduction in serum glucose and insulin concentrations.
Anticonvulsant activity may be due to a combination of potential
mechanisms: blocks neuronal voltage-dependent sodium channels,
enhances GABA(A) activity, antagonizes AMPA/kainate glutamate
receptors, and weakly inhibits carbonic anhydrase.
If you are going to buy Topamax or Topiramate without having a prescription, it may be a good idea to read users reviews first.
Last updated: February 10, 2015


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Prime Property
Blogging Houston real estate with Nancy Sarnoff and the Houston Chronicle real estate team.
Posts filtered on Tag
Historic building on Bellaire eligible for national registry
David Bush, deputy director of Preservation Houston, reports that the Texas Historical Commission has determined that the former Shell Oil Exploration & Production Research Laboratory is eligible for listing in the National Register of Historic Places, the nation's official roster of historic properties worthy of preservation. That means if a buyer were to rehab the […] [Read More]
Unique housing project rising in Spring Branch
This stretch of close-together single-family homes — each with an individualized look — stands out in a Spring Branch neighborhood of older, moderate-income housing. InTown Homes is developing the project, called Kolbe Farms, which appears to be following the guidelines of new urbanism with front porches and rear garage access. On its website, the developer […] [Read More]


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Unlocking the Key to Deep Sleep
By Sarah Fenske
Making the Ferguson Commission Matter
By Danny Wicentowski
My Fake Boyfriend Became a Real-Life Crush
By Lindsay Toler
Rep. Jeffrey Pogue Bill Would Ban Unisex Bathrooms
By Danny Wicentowski
Darren Wilson Gives Speech to Nonprofit
By Danny Wicentowski
Angel Stewart and Other Victims of Violence Ask for Mercy
By Jessica Lussenhop
Obama Praises St. Louis' LaunchCode
By Lindsay Toler
By Lindsay Toler
"That was a human being they shot, not some animal on the street," says Barry Laboube (pronounced "Laboo"). A construction worker for most of his 49 years, Barry looks far from delicate, yet he trembles as he talks about his only son. Barry keeps repeating, "He knew better. It was just a stupid thing that happened."
It is midafternoon, the Friday before Halloween, exactly a month since Jason was killed. Barry has just come from the foot of New Market Street, down near the river, where he works as a crane operator. At a table just inside the door of Parodi's, a bar and grill at Ninth and Tyler streets, Barry brings out a pack of pictures. There's a beaming, dark-haired boy of 9, hugging a dog. "He had chickenpox," says Barry, explaining the spots on Jason's face. "And that's Max. He loved that dog."
There's Jason as a Tiger Cub in Troop 585, Arnold. An older Jason in the beige uniform of the Boy Scouts. "He had 31 merit badges," says Barry, who was an assistant scoutmaster in his son's troop. "Jason was a Life Scout and only had to do his project to make Eagle, but he lost interest. The cars and the girls took over." Jason was buried with those merit badges, says Barry, along with a Moon Pie; Moonpie was a nickname, somehow connected to a bawdy scene in a movie. And there's Jason in another uniform, that of a drum-and-bugle corps, in which he played snare drum.
Finally there's Jason standing in the driveway with his first car, a 1990 Chevy Cavalier, a 16th-birthday present from his folks, Barry and Barbara. After Jason totaled the Cavalier, he got an '85 Ford Mustang. "We ended up having to garage that car," says Barry. "Jason lost his license for a year -- too many tickets. But he still got around. He had a bunch of friends -- I mean a bunch. He never lacked for transportation -- the phone was always ringing."
When teenage boys change, as they sometimes will, from pliant and respectful to obstinate and surly, it is usually a gradual transition. It's rare that a parent can pinpoint a particular incident that signals the phase, but Barry believes he knows when Jason started down the path that dead-ended in Marine Villa. At 15, Jason had a skateboard accident, and Barry thinks the accident altered his son's state of mind: "He suffered a concussion and was in the hospital for three days. After that, he started having problems. He became depressed, and he was having problems at school. We took him to a counselor, and the counselor said he had attention-deficit disorder. We think it was around this time, his sophomore year, that he turned to drugs, mainly pot."
Jason was caught up in the whirlwind of high-school society, especially after he got his license and his car. There were parties on weekends and an ever-rotating roster of new friends. He loved the raves (all-night alcohol-free dance parties where ecstasy, Special K [ketamine, an anesthestic] and other choice drugs of the culture are freely available) in St. Louis' Loft District downtown and kept his feelers out for any news of one. Even after he lost his license, he found ways to get to the city and rave. "His friends were always coming by," says Barry. "We didn't know the good ones from the bad ones. You don't know. You've got to give them freedom, but how much? You can't constantly supervise."
Even without full-time surveillance, however, Jason's parents couldn't help but notice the smell of pot. And so it was that while Jason was still in high school, Barry and Barbara began looking into a rehab facility for their son, settling on the Highland Center. "He had a problem with pot," says Barry. "I know he wanted to stop, but peer pressure's a hard thing."
Jason straightened out enough to graduate with the class of '99 from Seckman Senior High School in Imperial. There is no record of his involvement in any extracurricular activities.
By the fall of 1999, many of Jason's friends had gone on to college or enlisted in the military. Jason tried to join the Army in December but was turned down because of his asthma. Eventually he enrolled in ITT Technical Institute in Arnold. "He loved music," says Barry. "He went to concerts, and he wanted to work the soundboard, the mixer, as a career. But he didn't apply himself at that school. He had dropped out, though at the time of his ... his death, he intended to go back."
Around this time, Barry and Barbara, alarmed by Jason's continued pot use, decided that he needed a shock to the system. It happened at home. "It comes to a point," says Barry, "you don't know what to do, and that's the point we were at. We called the police, and they came and took him in, and when they searched him, they found a small amount of marijuana.
"Oh, he was mad," says Barry, recalling Jason's reaction at being turned in, "but we were trying to get him to stop what was going on. He was a good kid, but he was taking the wrong road."
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Toys & Hobbies
Limited Edition Sphero 2.0An Apple exclusive, Limited Edition Sphero 2.0 Revealed features a custom shell and inductive charger that showcases Sphero's inner robot. Control this amazingly smart - and smart looking - toy with your iPad, iPhone or iPod touch and access more than 25 apps for a mesmerising experience.€129.95
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Who We Are
Engineers and Constructors International, Inc. (ECI) is a multi-disciplined consulting firm with over 35 years experience in refining, chemical plant engineering, & design located in Baton Rouge, Louisiana. ECI can provide project support for projects of varying sizes, ranging from conceptual process design through detailed engineering, procurement, and construction management (EPCM) services, ranging from small maintenance / revamp projects to new grass-roots plant projects. ECI also has a professional services division which provides plant personnel staffing to support engineering, operations, and maintenance departments.


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A machine gun sound effect I made combining various sounds from my own library + some synthetic elements from a ...
A cool and stereotypical sounding machine gun sound I created with Matt_G's M240 machine gun sound and a lot of ...
Machine gun fire
Heavy machine gun burst.
Three versions in the following order -Far Away -Near the gun -Inside of the gun
A processed sound from "erdie lanz bulldog tractor engine outside" with looping on the tickle
a bit of contact mic action and a hair dyer
submarine/underwater sound
Two bodies or object splash into the water, as heard from underwater. Uses Noisecollector's hydrophone recording and ngarvey's ocean surf ...
This launch is a filtered version of Torpedo launch with some phaser added to simulate the water sounds.
SFX. Sth. heavy droping with long echo. Also may sounds like deep explosion.
Some explosion sounds I mixed up from various free web sounds i.a.
cannon shooting explosion midwayfield recording
Cannon getting shot. Extracted from a video () by a US Government agency, thus public domain. This sound is cc0/public ...


text: Trailer Kings Reality Show 04/14/2014 08:31 AM 04/14/2014 08:48 AM Trailer Kings Reality Show 04/14/2014 08:48 AM Spencer Young and David Yelton join us to talk about their new show entitled "Trailer Kings." Copyright 2014 Nexstar Broadcasting, Inc. All rights reserved. This material may not be published, broadcast, rewritten, or redistributed. Page: [[$index + 1]] morning news morning show morningnews news top stories


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Product Features
One could write books about the DC Kupress Hoodie. Let me compare thee to a raincoat—waterproof and containing many secure pockets. Let me compare thee to a blanket—warm, soft, and fleecy. Let me compare thee to that kid at school everyone likes—effortlessly cool. Yes, this hoodie truly has it all, with its full zipper, adjustable hood, and contrasting middle panel—but the DC Kupress Hoodie is missing one thing, and that's a person stylish enough to wear it. Could it be you?
All Gear by: DC


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Tee shirts possess a lengthy historical past in the realm of style. Since the intro, in the event it was only employed since undergarments inside 1800s, fundamental T-shirts have got stapled by itself using the clothing of almost each home. With the
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- The Finer Things in Life - http://amysfinerthings.com -
Smile {A Fresh Start}
Posted By Amy On January 3, 2013 @ 12:16 am In Intentional Living | 8 Comments
"Mom, is that your mad face?"
"Mom, why do you have your mad face on?"
"Mom, is that your mad face or your concentrating face?"
Apparently I don't smile enough!
This one holds me accountable. He's a sensitive soul, and it worries him when the corners of my mouth aren't turned up. He doesn't want me to be upset or angry, and he certainly doesn't want to be the cause of the scowl!
Do I scowl?!
For a fresh start this year, I'm going to smile more.
Oh sure, the baby brings out the grins in all of us, and some of the things the toddler says have us leaving the room so he doesn't know we think he's funny. (Oh, but he is funny!)
In the day to day grind, though, I think I forget to smile! Pity, because it takes more muscles to frown than it does to smile, and I'm a bit of a lazy sort, so I should smile more! Besides, smiling is an instant mood lifter. It makes the ordinary extraordinary. Smiling is contagious, the best kind of sharing! We never know how a smile may change someone's day, even our own.
A smile may not fix my situation, but it very well could fix my attitude.
This year, I'm going to smile a big smile. A smile that can be seen on my face and heard in my voice. (Even my writing voice. Can you hear it? )
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Glee's Grant Gustin Lands New Indie Movie Role!
Grant Gustin and his hipster glasses are coming to a theater near you! Grant is best known for playing Sebastian on Glee, and his good looks and charm (oh, and acting skill) have helped nab him the title role in Affluenza — not to be confused with his other recent movie role: the Lifetime flick, A Mother's Nightmare.
Affluenza is a "modern day take on The Great Gatsby," and Grant plays Todd Goodman, a preppy blue blood. Unfortunately his wealthy parents fall victim to 2008's financial crisis and he's faced with a startling world where people don't pop their collars, and boat shoes aren't socially acceptable. Shocking, we know.
Affluenza is directed by Kevin Asch, with a screenplay by Antonia Macia. Production starts next week in-and-around New York City and Long Island.
Source: The Hollywood Reporter


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Jan 14, 2008
One of the funniest memories Mr. Flinger and I have about being first time parents, is the time we went to Borders Books with our 5 week old. We had the large diaper bag, the bjorn, the carrier, two blankets, binki, bottles, a lovey and I think, maybe, a baby with us.
People laughed at the amount of stuff we were carrying. We did not.
Over the years, I've been known to (let's say) carry too much?
Now, though, I'm pairing down. Diaper bags are getting smaller (a lot like the cell phone or MP3 player or a my brain capacity or hours of sleep per night). My purse is a wallet on a string. My children, godbless'em, if they piss themselves they are, as the saying goes, "S.O.L." But at least now I don't walk around with my shoulders haunched over like a Sherpa.
I wish I could say the same thing about the trunk of my car…
I have to say that the mountains of "Stuff" you have to haul around like a pack mule sucks a major duck. (You HAVE to be prepared, though!!!)
I cried tears of joy when I could carry a real purse again.
By Loralee on 2008 01 14
My husband was the one who insisted we needed to carry 10 tons of stuff for the baby in the big diaper bag. I finally showed him the merits of the little diaper bag. However I opened my trunk yesterday to stash my groceries and had to shove them between the pack and play and the stroller. I'm not sure how I will handle two kids.
By Someone Being Me on 2008 01 14
Sounds & looks familiar. Very familiar.
By AMomTwoBoys on 2008 01 14
Please tell me you got a new car. No way that much would fit in the back of mine!
Although that said I do use the car asa repository for things I can't fit in my backpack..blankets, towels, extra clothes, etc
By Carrie on 2008 01 14
Today I had to take out our canvas chairs (but kept the umbrella stroller) to make room for the All-Terrain Red Wagon…what we DID NOT use on our walk. It is still out there… and the diaper bag is non-existent anymore and someday my purse will be my stuff only!
By hilary on 2008 01 14
That could almost be a picture of my trunk! I know just what you mean…I carried everything from baby nail clippers to infants' Tylenol! Yeah, like I was going to clip my baby's nails at the mall or something!
But I could have if I WANTED TO!!!
By LeannIAm on 2008 01 14
I now just carry a purse and toss a Pull Up in and maybe a box of raisins, some hand sanitizer, and a travel thing of wipes.
And hey, wait a minute…that's MY car at this very moment!
By Jamie on 2008 01 14
ha ha ha ha.. thta looks familiar.. but where are the goldfish?
Jen R
By Jen Rizzo on 2008 01 14
My son BIT my iPod. Yep. It's got teethmarks in it! Several other geeks (including a guy at the Apple Store) have told me the same thing "oh you HAVE to save that." I agree. When it's obsolete I'm putting it into a scrapbook. I'm not sure how one would stand up to poop however.
My 'diaper bag' is my laptop backpack. It's got USB cables in one pocket and a sippy cup in another. It's about half and half the amount of geeky tech stuff compared to baby stuff for my son.
I am a sad case, I know.
By TheMacMommy on 2008 01 14
The car makes me cringe.
By Aimee Greeblemonkey on 2008 01 15
Oh we have definitely reduced the amount of stuff we take to go anywhere. Even when we had 3 in diapers we were able to reduce the size of the bag from the first baby days. It is amusing to see the first time parents that have brought the entire house with them for a one-hour outing to Mass or something, where the child is likely to sleep the entire time. I love watching new parents and remembering all of the new experiences.
The back of my car may well contain treasure, there is so much stuff in there.
By 4andcounting on 2008 01 15
It is so nice when the kids are old enough you don't have to drag the entire house out with you for a small trip!
then they just want to bring bigger things with them everywhere they go!
By Tuesday on 2008 01 15
I laugh now thinking how MUCH stuff we were sure we needed, with #1. After #4 was born, I gave up and just started leaving all the crap at OTHER people's houses ;o)
By Liz on 2008 01 15
I'm back to tell you that I still think the back of your car looks like mine…and to say that you've been given an award.
Check my blog for details…
By LeannIAm on 2008 01 15
If it were my car, you would also see cracker crumbs, animal crackers and juice boxes thrown all over.
By Sister Honey Bunch on 2008 01 15
there's nothing quite like the day that you don't need to bring ANY kid stuff with you. we are so close to that stage. for us we still need a sippy cup and a change of underwear and pants…
By ali on 2008 01 15
I over pack too. But, I have always said, "you never know what is going to happen". Well, we got stuck on the side of the rode in -10 degree weather and we were waiting for over 1.5 hours for a tow truck, and my husband looked at me and said, "thank god you packed." I had warmer clothing, my PIS Advanced, three bottles of breast milk and two frozen bags of it, lots of diapers, a blanket for the baby and four changes of clothes. I also had snacks for us and a blanket for us because I am always cold. After that my husband decided that we should have a duffel bag in the car with stuff just in case. LOL.
By KristinaB on 2008 01 15
once the boy child hit 6 months it was bye bye big snoopy diaper bag and hello nice sleek black 1/2 foot long diaper bag. It is so nice not having to carry two 22 lbs things at the same time while trying to hold a two year olds hands.
By Veronica on 2008 01 15
#1 - LOVE that little dotty bag! Do tell the details!!!!
#2 - I totally feel your pain. Now with 2 in diapers and one lazy mama, I will sometimes totally forget a diaper bag when I run somewhere. "Oh, you need a bottle? Well, I forgot to bring you one…want a french fry? Cappuccino? No? Oh well!"
By Southern Mom on 2008 01 15
The more children I had the less I carried around for them. Now? We leave the house with NOTHING except the kids. We even go out of town without changes of clothes—we like to play russian roulette that way. But Ben is finally potty trained. He hasn't had an accident other than at night in a couple of weeks and I can finally say that we are diaper free.
It's a wonderful state of being I have to tell you. But if it can't fit in my purse (when I choose to take my purse with me that is) then it doesn't need to go. I love that.
By Sleeping Mommy on 2008 01 16


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Full detailed profile on Jack Manning Played by Andrew Trischitta on One Life To Live Daytime Soap Opera.
Andrew Trischitta
Birthday: February 24, 1995
Jack gets irritated as Shaun follows him to the coffeehouse. He sees Kate and tries to talk to her about 'school'. She insists they talk in class then leaves. Jack pokes Shaun in the chest and warns him to stop following him. Shaun bends Jack's finger back and tells him to take his issues up with his father.
In Todd's suite, Blair tells him last night was a mistake. Todd knows he always ends up hurting her. Blair's scared of her feelings. Todd kisses her, and they make love again. He doesn't want to lose Blair. She kisses him. Later, Jack barges in and realizes Blair spent the night with Todd and screams at his parents.
Blair finds Jack at the mansion and admits she and Todd are getting back together. Jack refuses to accept it.
Jack spots Shaun watching him and Kate at the coffeehouse. Kate becomes paranoid, doesn't want to lose her job and leaves. Jack calls Blair and insists he was studying with his teacher and demands Todd call off his goons.
At the mansion, Dorian leaves a message for Frank then tells Blair Jack left really early. Blair's confused, as Jack's usually the last one up. She calls Jack, who insists he's at the coffeehouse when he's really in bed with Kate. After Blair hangs up, Dorian rants about David and that exotic floozy, Rama. Blair assures Dorian that Rama isn't a threat. Later, Nora arrives, and Dorian insists she open an investigation on Viki and her Pellegrino Fund involvement.
Jack stares at Kate in English class, as she asks him in front of the class what his favorite workout song is. She tries to teach how poetry and rhythm can blow your mind. Kate keeps Jack after class. He's uncomfortable and suggests transferring classes. Kate kisses him.
In a room, Jack apologizes to the girl for their sex not lasting longer. They have sex again. Afterwards, she introduces herself as Kate. Does she always pick up guys like this? Kate claims this is the first time, as Jack almost reveals he was a virgin. Jack notices the time and has to leave. His mom's going to kill him! He backtracks, trying to act older, and gets Kate's number.
Jack takes a seat in English class and is shocked to see Kate is his summer school teacher.
Before Jack leaves the mansion, Blair urges him to be careful. "I know," Jack says. "Stranger danger." He brings up Todd and hopes Blair isn't going to get back together with him. After Jack leaves, Blair gets a call from Todd, who updates her on Victor's whereabouts.
Jack notices an older woman smiling at him at the coffeehouse. He looks away then back at her, but she's gone. She appears over his shoulder, takes his laptop and walks out the door. Jack follows. Outside, Shaun gives Destiny money and wishes Matthew would help out more. Destiny thinks things are getting better. He's impressed to hear Destiny's thinking about dating. Later, Matthew and Jeffrey arrive and are surprised to see Michelle walk into the coffeehouse.
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