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The two consoles we landed on were the Midas M32 and the Soundcraft Si Expression 3. The rep from Soundcraft wasn't able to get his hands on an Expression 3, but he did send an Expression 1, which is basically the same desk with fewer faders and physical inputs than the 3. For our purposes it was an adequate stand-in. The folks from Midas sent two sales reps and a brand new console still in the box. Soundcraft sent the console but no rep.
The console we will choose has to meet a few simple requirements. Many of the ones we looked at meet some or all of the requirements, but these two were the ones we thought were best.
Here's what we are looking for in no particular order:
- Ease of operation – Most of these consoles will be run by volunteers who don't have ridiculous amounts of time for training and getting to know the board. They need to be able to walk in and get started as soon as possible.
- Build quality – We need these things to last for a long time. We're not rough on our gear, but we do like to keep it around as long as possible.
- Sound Quality – It doesn't matter if they're easy to learn and built like a tank if they sound terrible. We need them to sound good.
- Cost – We're looking to buy a few of these and the lower the cost, the better.
Walking into the test, I thought I had a good idea of what we would find. I thought the Soundcraft board would "win" because at face value, it looked like the easier board to run. Fewer knobs, less screens to go through to get what you want. It just looked like an easy board for a volunteer to operate. After the test, I had a different opinion.
We looked at the M32 from Midas first. Since their reps were there, they unboxed it and set it up for us. As the rep started going through the setup and operation of the console one thing became very clear – they nailed it with the operating system on this console. The firmware that originated in the Behringer X32 really shines in the Midas chassis. No matter what you're trying to do on this console, it seems that the correct screen is either already engaged, or is one button click away. Very nice. Setting up mix groups, monitor sends, etc. is dead simple. Just a few very intuitive clicks and you have a drum group, vocal group, or whatever type of group you want ready to go. Really easy. The key word here is "intuitive". Everything about the layout and operation of this console makes sense. That's strong praise from an old video guy.
After we wrapped up our demo of the M32, we moved on to the Soundcraft Si Expression. Let's just say that it's not super intuitive to operate. Lots of the same functionality is there, but figuring out how to get it to do what you want is kind of a challenge. With some time and a little bit of monkey-punching we got it do do what we wanted but never in a way that was as easy as the M32. Throw in the lack of scribble strips and limited screen real estate and you have some real shortcomings compared to the Midas board.
At the end of the day, it was an easy decision for us. The M32 had a fuller sound and was much easier to operate. Despite the fact that it costs about $1500 more than the Soundcraft, we landed squarely on the M32. You won't see it at Front of House in our main room any time soon, but in the medium-sized rooms we are looking to outfit, it's the perfect choice.
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Los Angeles Lakers v Toronto Raptors
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Steve Nash of the Los Angeles Lakers drives against Jose Calderon of... Fotografía de noticiasAir Canada Centre,Baloncesto,Basket-ball - NBA Pro,Canadá,Deporte,Encuadre de cuerpo entero,José Manuel Calderón,Lanzar,Los Angeles Lakers,Steve Nash,Toronto,Toronto Raptors,VerticalPhotographer Ron TurenneCollection: National Basketball Association 2013 NBAETORONTO, CANADA - JANUARY 20: Steve Nash #10 of the Los Angeles Lakers drives against Jose Calderon #8 of the Toronto Raptors on January 20, 2013 at the Air Canada Centre in Toronto, Ontario, Canada. NOTE TO USER: User expressly acknowledges and agrees that, by downloading and or using this photograph, user is consenting to the terms and conditions of the Getty Images License Agreement. Mandatory Copyright Notice: Copyright 2013 NBAE (Photo by Ron Turenne/NBAE via Getty Images)

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Hotels in 27959 - Nags Head NC
Only hotels in 27959 zip code are listed below. Search for cheap and discount hotel rates in Nags Head, NC for your upcoming leisure or conference / group travel. We list the best 27959 hotels and motels so you can review the Nags Head hotel list below to find the perfect lodging. Hotels in Dare county and area code 252 are also listed.
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Recent Appearances
Ballistic Missile Proliferation Control
Experts talked about the state of ballistic missile proliferation of Iran, North Korea and other countries. They…
Missile Defense Systems
Participants spoke about European attitudes toward U.S. plans to develop a missile defense program. Among the topics…
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Miscues spoil Hammerheads' title hopes
CORPUS CHRISTI - Louisiana left little doubt about which team was the best in the Intense Football League. But, just in case there were any problems, the Hammerheads gave a little help to the Swashbucklers Saturday night.
Corpus Christi was denied its elusive indoor league title, falling 46-27 to Louisiana in Intense Bowl III at the Lake Charles Convention Center. In bowing out in the title game for the second season in a row, the Hammerheads did as much to hurt their championship chances as anybody on the Swashbucklers.
A penalty wiping out a touchdown. A failed fourth-down goal-line plunge and a goal-line fumble. Missed kicks. A safety after a poor snap on a field-goal attempt. All those Corpus Christi errors hurt.
As the pass defense was riddled by Louisiana quarterback Freddie Harrison, there was no relief for the Hammerheads.
Louisiana (15-1) fell only to Odessa this season and handed Corpus Christi (13-3) all three of its losses on the way to its first league title.
Going against the league's top-rated defense and being held to 115 total yards through the first three quarters, the Hammerheads did themselves no favors.
Corpus Christi had drives stall out three times in the first half, twice missing field goals and fumbling the other time. There also was a blocked extra-point when the team did reach the end zone.
The most critical miscue came at the end of the first half. Down 24-14, Hammerheads back Fred Wallace had a touchdown run erased by a penalty. The drive ended without a score when kicker Rene Vasquez missed from 29 yards.
After successfully converting the first three fourth-down attempts of the game, the first failure truly hurt. On fourth-and-goal with Corpus Christi trailing 34-20 with seven minutes left in the third quarter, the 300-pound-plus Wallace was stuffed short of the end zone, as the Hammerheads could not score during the quarter.
It took only a few plays for Harrison to hit Keith Smith for his fifth -- and last -- touchdown pass of the game.
On third down on the next series and Corpus Christi threatening again near the Louisiana end zone, Wallace coughed up the ball, ending the drive and all but ending the game and the team's Intense title hopes.
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Coatings, revisited
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Coatings, revisited
For all those concerned about whose coating is best or most durable, I thought I'd put this picture up. This is an Evans G14 that I used for one 3-hour rehearsal. You can clearly see holes already wearing in the coating, and it is smoother to the touch in the center.
Now, I don't intend to bash ANY company, because my opinion is that coatings are going to wear, it is a natural thing, and that is the experience I've had with Remo, Evans, and Aquarian. I have read on this site countless times about how lousy Remo's coatings are, and how an Evans head would still look brand new, etc. And I just wanted to share that this NOT my experience. I tend to think that those who strongly tout one brand or another tend to have a bit of fanboy-ism going on. (Just kidding. Sort of...) I know we all have preferences and I'm cool with that. My point is, choose the sound you like and don't worry what someone else says about coatings.
For the record, the head sounds great, and I'm totally satisfied with it. I wanted to try a single ply alternative to the Emperor, and thought I'd give the Evans a whirl.
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A chromogenic solution for enzyme immunoassay. A stabilizing agent is added to chromogenic solutions containing chromogen of the hydrogen donor type. The stabilizing agent is an alkylhydroxylamine, preferably N,N-diethylhydroxylamine. The N-N diethylhydroxylamine retards degradation of the solution of the chromogen in solvent and is particularly effective in retarding discoloration of a chromogen-solvent solution placed in storage over an extended period of time. The alkylhydroxylamine preferably in the form of N,N-diethylhydroxylamine has the additional property that it does not adversely affect the activity of enzymes employed in enzyme immunoassays. It also does not interfere with binding specificity or reactivity of antibodies.
1. In an enzyme immunoassay for the colorimetric detection of an antigen comprising the steps of adsorbing a quantity of a first antibody to a solid support; forming a conjugate between an immunologic reagent and an oxidoreductase enzyme; mixing said conjugate with a sample to be tested for an antigen so that said antigen binds to said first antibody and to said conjugate to form an immunologic complex in solid phase; determining the presence of said enzyme in the adsorbed material by subjecting the adsorbed material to a chromogenic substrate comprising a chromogen which is a hydrogen donor; and monitoring the visible color characteristics of said substrate; the improvement comprising that said chromogenic substrate is provided in a chromogenic solution comprising a chromogen, a solvent and a stabilizing agent comprising an alkylhydroxylamine which is soluble in the chromogenic solution and which functions to retard discoloration of the chromogen during storage of the chromogenic substrate prior to use in said assay, said stabilizing agent being present in an amount sufficient to stabilize said chromogen and having the additional properties that it does not adversely affect either the reactivity of the enzyme or the binding specificity and avidity of the first antibody and said conjugate for said antigen.
2. An enzyme immunoassay as in claim 1 wherein the stabilizing agent is N,N-diethylhydroxylamine.
3. An enzyme immunoassay as in claim 2 wherein said N,N-diethylhydroxylamine is present in said solution at a concentration in a range between about 1 part N,N-diethylhydroxylamine to 50,000 parts solution up to about 1 part N,N-diethylhydroxylamine to 200,000 parts solution.
4. An enzyme immunoassay as in claim 1 wherein the chromogen is a tetramethylbenzidine.
5. An enzyme immunoassay as in claim 1 wherein the enzyme is a peroxidase.
6. A diagnostic kit for carrying out an enzyme linked immunosorbent assay for detection of an antigen wherein said kit is suitable for home diagnostic and clinical application and is to be employed and stored under ambient room temperature conditions, said kit comprising (a) a solid support precoated with a first antibody; (b) a solution comprising a conjugate of an oxidoreduclase enzyme with a second antibody; and (c) a separately contained chromogenic solution responsive to an oxidoreduclase enzyme for said immunosorbent assay, said chromogenic solution comprising a chromogenic substrate having the properties of a hydrogen donor; wherein the improvement comprises that said chromogenic substrate is in the presence of a stabilizing agent comprising an alkylhydroxylamine functioning to retard discoloration of the chromogen during storage of the chromogenic substrate prior to use in said assay, said alkylhydroxylamine having the properties that it is soluble in the chromogenic solution, that it does not adversely affect either the reactivity of the enzyme or the binding specificity and avidity of any immunologic reagent for said antigen.
7. A diagnostic kit as in claim 6 wherein the stabilizing agent is N,N-diethylhydroxylamine.
8. A diagnostic kit as in claim 7 wherein the chromogenic substrate is provided in a chromogenic solution comprising a chromogen and solvent and the N,N-diethylhdroxylamine in said solution has a concentration in a range between about 1 part N,N-diethylhdroxylamine to 50,000 parts solution up to about 1 part N,N-diethylhdroxylamine to 200,000 parts solution.
9. A diagnostic kit as in claim 6 wherein the chromogen is a tetramethylbenzidine.
10. A diagnostic kit as in claim 6 wherein the enzyme is a peroxidase.
11. A chromogenic solution for use in an enzyme immunoassay comprising a chromogenic substrate having properties of a hydrogen donor, and a solvent, said chromogenic solution being capable of reacting with a peroxide in the presence of said enzyme in said immunoassay to produce a chromophore having visible color characteristics, wherein the improvement comprises the presence in said chromogenic solution of a stabilizing agent comprising an alkylhydroxylamine which is soluble in the chromogenic solution and which functions to retard discoloration of the chromogenic solution during storage prior to use in said immunoassay, said stabilizing agent having the additional properties that it does not adversely affect either the reactivity of the enzyme or the binding specificity and avidity of immunologic reagents in said immunoassay.
12. A chromogenic solution as in claim 11 wherein the stabilizing agent is N,N-diethylhydroxylamine.
13. A chromogenic solution as in claim 12 wherein the N,N-diethylhydroxylamine in said solution has a concentration in a range between about 1 part N-N diethylhydroxylamine to 50,000 parts solution up to about 1 part N-N diethylhydroxylamine to 200,000 parts solution.
14. A chromogenic solution as in claim 11 wherein the chromogenic substance is a tetramethylbenzidine.
15. A chromogenic solution as in claim 11 wherein the enzyme is a peroxidase.
This is a continuation, of application Ser. No. 721,102, filed Apr. 8, 1985, entitled CHROMOGENIC SOLUTION FOR IMMUNOASSAY, now abandoned.
1. Field of the Invention
This invention relates to biological diagnostic test kits. The invention particularly relates to stabilizers for chromogenic substrates used in enzyme-linked immunosurbent assays.
2. Description of the Background Art
Biological diagnostic test kits, prepackaged assemblages of assay materials, are gaining in popularity, particularly with the advent of the "home" test kit. These kits can be applied to human, veterinary or agricultural testing to detect a wide variety of conditions, commonly by employing antigen specific antibodies, produced in quantity from clones.
Prior to the introduction of test kits, laboratories typically performed these tests using fresh reagents as they were needed. A problem attendant with test kits however, is reagent destabilization as the reagents are left in storage. The mechanism by which destabilization can gradually occur is not well understood. Although gradual environmental oxidation may be a factor, there are likely other contributing factors as yet not understood which may be promoting the destabilization process. The assembled kits may remain on wholesale or retail shelves for extended periods of time, after which they may be stored for even longer periods by the end user. Moreover, storage conditions may often be harmful, for example, under elevated temperatures. These conditions can damage the reagents, rendering test results difficult to interpret, and generally impairing the reliability of the test.
One form of reagent degradation is the discoloration of chromogenic substances to be used in connection with colorimetric enzyme immunoassays. Chromogenic substances particularly those of the hydrogen donor type have a tendency to destabilize when left in storage over long periods of time. This results in a slight color change, typically a yellowig of the chromogenic which occurs gradually over an extended period. Some chromogenic materials, e.g. tetramethylbenzidene; and have been discovered to destabilize less rapidly than others; nonetheless, even this latter chromogen can undergo some discernible yellowing when left in storage for very long periods, particularly under hot environmental conditions. Destabilization of the chromogenic material resulting in yellowing of the chromogen is undesirable, since it can affect the end color change of the chromogen as it reacts to produce a chromophore during conduct of the immunoassay.
Commonly assigned U.S. Pat. No. 4,503,143 discloses an assay which functions as a test kit. The assay is an ELISA test, using tetramethylbenzidine (TMB) as a chromogenic substance. This assay functions quite well, rendering a clearly visible blue color as a positive test result. However, when the kit is stored for very long periods, for example longer than several years and under hot storage conditions, the TMB solution which is normally clear can develop some discernible yellow tinge. The yellow tinge, although slight, poses a potential disadvantage in that it can cause a green tinge to appear in the normally blue chromophore. The green tinge, if pronounced, could possibly be misinterpreted by an inexperienced user.
U.S. Pat. No. 4,067,690 discloses the addition of minute amounts of hydroxylamine to boiler water to retard corrosion due to dissolved oxygen. The hydroxylamine is intended for use in high pressure--high temperature boiler water. The hydroxylamine functions as an oxygen scavenger under these elevated operating conditions to retard corrosion of boiler metal surfaces, generally iron and steel.
U.S. Pat. No. 4,386,224 is directed to use of N,N-diethylhydroxylamine to stabilize color and inhibit color formation in monoalkyl phenols which frequently discolor with age. The hydroxylamine is added in amounts ranging from about 5 parts per million to about 50 parts per million of the alkyl phenol. This reference also discloses that the N,N-diethylhydroxylamine can reduce the color of monoalkyl phenols in which color development has taken place.
Accordingly, it is an object of the invention to retard degradation and discoloration of a chromogen during storage.
It is an object of the invention to provide a stabilizing agent for the chromogenic solutions to be used in enzyme immunoassays wherein the stabilizing agent retards discoloration of the chromogenic solution over an extended period of time in excess of one year.
It is a further object to provide a stabilizing agent to retard discoloration of chromogenic solutions of the hydrogen donor type to be used in colorimetric enzyme immunoassay kits.
In accomplishing the foregoing and related objects, the invention provides a diagnostic test kit comprising antibody-enzyme complexes, a chromogen, and a chromogen stabilizing agent, and an oxidizing agent for the chromogen responsive to the enzyme.
In accordance with one aspect of the invention, the chromogen is tetramethylbenzidine. This chromogen is oxidized in an enzymatically induced reaction with hydrogen peroxide to form a chromophore exhibiting a visually distinct blue color.
In accordance with another aspect of the invention, a chromogen stabilizing agent is added to prevent destabilization of the chromogen in solvent during extended storage periods, or when the chromogen-solvent solution is stored under elevated temperatures. An effective amount of an alkylhydroxylamine is added to the chromogen solution in the range of 1:50,000 to 1:500,000 parts by volume, preferably 1:50,000 to 1:200,000 parts by volume, which prevents chromogen degradation. N,N-diethylhydroxylamine (DHA) for example, has been found to be a particularly effective compund, which is soluble in the chromogen-solvent solution. It has also been determined that the addition of N,N-diethylhydroxylamine to the solution of chromogen and solvent will quickly reverse any yellowing discernible therein to result in a near colorless chromogenic solution.
A principle advantage discovered in the use of an alkylhydroxylamine as stabilizing agent for the chromogenic solution is that the alkylhydroxylamine does not adversely affect the reactivity of immunoassay components with which the chromogenic solution will ultimately come into contact. Specifically, the alkylhydroxylamine in above stated concentration has been found not to adversely affect the reactivity of enzymes, for example peroxidase typically employed in enzyme linked immunosorbent assays or the binding properties of antibodies and antigens employed in such assays. Additionally, the alkylhydroxylamine in the above stated concentration does not inhibit the oxidizing properties of peroxides e.g. hydrogen peroxide, which are normally added to the chromogenic solution as oxidizing agent in order to activate the chromogenic solution just prior to use in the assay.
The alkylhydroxylamine, preferably N,N-diethylhydroxylamine when added to the chromogenic solution has been discovered to stabilize the chromogen permitting the chromogen to be stored for at least one year, typically at least several year's duration without noticeable yellowing of the chromogenic solution occuring during the storage period.
This desirable result is realized when chromogens of the hydrogen donor type, particularly tetramethylbenzidine are employed. The preferred concentration of the N,N-diethylhyroxylamine in a chromogenic-solvent solution wherein the chromogen is a tetramethylbenzidine has been determined to be in a range between about 1 part by volume N,N-diethylhydroxylamine to 50,000 parts by volume chromogen-solvent solution up to about 1 part N,N-diethylhydroxylamine to 200,000 parts chromogen-solvent solution.
A more preferred concentration wherein the chromogen is a tetramethylbenzidine has been determined to be in a range between about 1 part N,N-diethylhydroxylamine to 100,000 parts chromogen-solvent solution to 1 part N,N-diethylhydroxylamine to 200,000 parts chromogen-solvent solution.
In accordance with the invention, an enzyme immunoassay procedure and testing kit is provided utilizing antibody-enzyme complexes a chromogenic substrate responsive to the enzyme, and a chromogen stabilizing agent. The chromogen stabilizing agent serves to prevent undesirable degradation manifested by discoloration of chromogen without interfering with the reactivity of the enzyme or binding specificity and avidity of the antibodies. The stabilizing agent of the present invention is particularly applicable to colorimetric enzyme immunosassays to be provided in the form of a diagnostic testing kit. The stabilizing agent of the invention has preferred application to chromogenic solutions containing chromogens of the hydrogen donor type for example 0-phenylenediamine (OPD), O-tolidine and particularly tetramethylbenzidine. A typical application of the stabilizing agent is as an additive to a chromogenic solution to be included as a component of an enzyme linked immunosorbent assay diagnostic test kit.
Typical chromogenic substrates employed in colormetric enzyme immunoassay to which the present invention applies are chromogens of the hydrogen donor type. A particularly advantageous chromogen is tetramethylbenzidene. When TMB chromogen is employed in an enzyme immunoassay, it oxidizes in the presence of an enzyme, typically a peroxidase, and a peroxide oxidizing agent. The resulting product is a chromophore having an easily discernible blue color. This indicates the presence of test antigen being assayed, since the antigen becomes linked to an antibody-enzyme complex of the immunoassay. To prevent visible degradation, i.e. discoloration of the TMB during prolonged storage of the test kit, the invention provides for the inclusion of a stabilizing agent in the form of an alkylhydroxylamine. The alkylhydroxylamine is added to the chromogenic solution which typically contains a chromogen and solvent. The chromogenic solution with alkylhydroxylamine included therein is kept as a separate component of the test kit until time for conduct of the assay. The stabilizing agent of the invention has been determined to play an important role in colorimetric enzyme immunoassay test kits wherein storage durations have been prolonged or environmental conditions harsh, causing some visible discoloration of the chromogen prior to use.
A preferred stabilizing agent for the chromogen which has been discovered to be particularly advantageous is N,N-diethylhydroxylamine. This alkylhydroxylamine is a particularly effective stabilizing agent for tetramethylbenzidine (TMB). It has been discovered that minute addition of N,N-diethylhydroxylamine to TMB chromogenic solutions retards the rate of discoloration so significantly that yellowing of the chromogenic solution is either imperceptible to the naked eye or barely perceptible even after the chromogenic solution is left in storage over one year's time and subjected to elevated environmental temperatures during this period.
The N,N-diethylhydroxylamine has been determined to be a particularly advantageous stabilizing agent for tetramethylbenzidine, since it does not adversely affect the reactivity of an oxidoreductase enzyme, typically a peroxidase, employed in the assay. It also does not interfere with binding specificity or avidity of the antibodies employed in the assay and does not interfere with the oxidation of the chromogen to chromophore by the admixture of peroxide into the chromogenic-solvent solution at time of use. Common biochemical antioxidants for example sulfur containing antioxidants have been found to be unsuitable in the present application, since they do not retard yellowing of chromogen without also adversely affecting the reactivity of the other assay reagents.
An effective concentration range of the N,N-diethylhydroxylamine (DHA) in the chromogen solution which accomplishes the foregoing results has been determined to be in a range between about 1 part by volume DHA to 50,000 parts by volume chromogen-solvent solution up to about 1 part by volume DHA to 200,000 parts chromogen-solvent solution.
A preferred formulation for an activated tetramethylbenzidene chromogenic solution with the preferred stabilizing agent of the invention added thereto is illustrated in Example 1: The following examples incorporates subject matter from commonly assigned U.S. Pat. No. 4,503,143 herein incorporated by reference (U.S. No. 4,503,143 makes no mention of use of an alkylhydroxylamine additive.)
The following mixure, exclusive of the use of N,N-diethylhydroxylamine additive, appears in commonly assigned U.S. Pat. No. 4503143 herein incorporated by reference. An activated TMB chromogenic solution was prepared by mixing 4.0 parts by volume of reagent (i) with 11.0 parts by volume of reagent (ii), and then adding 0.010 part by volume of the 30 percent hydrogen peroxide solution (reagent (iii)). The mixture was stirred to form a homogeneous, activated TMB solution. The individual reagents were produced as follows:
Reagent (i) was prepared by dissolving 1.25 g (5.20 mMol) of 3,3,',5,5'-tetramethylbenzidine, in 1.00 liter absolute methanol with or without heating. Diethylhydroxylamine was added to the TMB/solvent solution to a concentration of 1 part by volume N,N-diethylhydroxylamine to 100,000 parts by volume of the TMB/solvent solution. A clear colorless or faintly tan solution resulted which could be stored for at least 1 year at 4 to 25 C, in a brown bottle without affecting its usefulness in an enzyme immunoassay.
Reagent (ii)
Reagent (ii) is a buffer prepared by first dissolving 144.8 grams (1.020 mol) of disodium hydrogen phosphate in 1.00 liter hot deionized water. The phosphate dissolved in the hot deionized water upon stirring. To this solution 102.95 grams (0.4902 mol) of citric acid monohydrate were added. The resulting solution was then diluted to 10.0 liters with additional deionized water, thus forming a citrate-phosphate solution, with a pH of 5.0.
Reagent(iii) consisted of an aqueous solution of hydrogen peroxide, wherein the hydrogen peroxide comprised 30 percent by volume.
Applications of the improved chromogenic solution employing N,N-diethylhydroxylamine additive for use in preferred enzyme imunoassay are illustrated in the following examples.
The following protocol is not limited to specific types of antigen; however, it has been found particularly suitable as a double sandwich enzyme immunoassay for human luteinizing hormone (HLH) and human chorionic gonadotropin hormone (HCG). The procedure involved coating the support plate with a first antibody, adding the antigen sample, and then a second antibody-enzyme conjugate. The first and second antibodies in the various assays were obtained from monoclonal hybridoma antibodies prepared from inoculated mice or polyclonal antisera generated by the inoculation of rabbits with the test antigen. The antisera were purified by successive precipitations with ammonium sulfate.
Wells of a polystyrene microliter plate were coated with the first antibody: a coating antibody solution was added to each well, and this was incubated for 1-4 hours at room temperature, then decanted. Three hundred microliters of a PBS/0.5% BSA blocking solution (0.5 gm bovine serum albumin per 100 ml of phosphate buffered saline) were added to the wells and incubated for thirty minutes. The wells were then decanted and twice washed with PBS/Tween buffer. The PBS/Tween buffer was composed of 0.1 vol. percent Tween Solution and 99.9 vol. percent PBS.
A solution to be tested for antigen was added to the wells and incubated. The incubation times were thirty minutes for HCG and one hour for HLH. The wells were then decanted and washed with PBS/Tween buffer.
A solution of horseradish peroxide-conjugated second antibody, which may typically be conjugated with glutaraldehyde or avidin-biotin was added to the wells, and incubated for thirty minutes at room temperature. After decanting and washing with PBS/Tween buffer, 150 microliters of the TMB activated solution of Example 1 were added.
The activated chromogen solution was made in accordance with Example 1, by mixing 4.0 parts by volume of reagent (i) with 11.0 parts by volume of reagent (ii), and then adding 0.010 part by volume of the 30 percent hydrogen peroxide solution (reagent iii). The mixture was stirred to form a homogeneous, activated TMB solution which contained N,N-diethylhydroxylamine in the concentration specified in Example 1. The chromogen-containing solution was allowed to incubate for between 5 and 30 minutes at room temperature. Then an absorbance reading was taken at 660 nanometers (nm) using a Dynatech Microlisa MR 580 Autoreader, available from Dynatech Laboratories, Alexandria, Virginia. The protocol set forth in Example 2 was used successfully to detect HCG hormone at concentrations as low as 7.8 nanograms/ml. and HLH at concentrations as low as 7.8 nangorams/ml.
The rate of color development, as measured by change in absorbance of the activated TMB solution at wavelength of maximum absorbance, 660 nm, was at least three times greater than that achieved with the use of the chromogen o-phenylenediamine (OPD) dissolved in its optimun hydrogen peroxide concentration. The absorbance of OPD color change was also measured at its wavelength of maximum absorbance.)
The double-antibody sandwich ELISA assay of Example 2 was repeated for HCG with the following modifications: The antigen solution and enzyme-conjugated second antibody were added and incubated essentially simultaneously in the wells coated with the first antibody. Specifically the antigen solution and enzyme-conjugate were first admixed and essentially immediately thereafter the mixture was added to the wells coated with first antibody. The mixture was incubated in the antibody-coated wells for a period of thirty minutes at room temperature. This abbreviated assay provided results comparable to those of Example 2, and resulted in at least three times faster color development than would occur if OPD chromogen were used in this same assay under optimal hydrogen peroxide concentration. The protocol set forth in Example 3 was used successfully to detect HCG hormone at concentrations as low as 7.8 nanograms/ml. (urine).
The assay methods set forth in the above general description and illustrative examples may be applied in a home diagnostic assay kit. For example, a number of vials containing the various immunologic reagents required for the assay may be included in such a kit. The user need then only mix these reagents with the test sample in accordance with a given protocol and await a color change in the final solution. One method of application of the double antibody sandwich ELISA to a home diagnostic kit could be effected, illustratively, by providing the kit with one vial (vial 1), which has been precoated with a first antibody and blocking solution and a second vial (vial 2) which would contain the second antibody--enzyme conjugated preferably in lyophilized form. A third vial (vial 3) could contain a solution of the chromogen and solvent, and a vial 4 could contain the solution of buffer and hydrogen peroxide.
In the carrying out the assay, the user need only collect a sample (for example a urine specimen) suspected of containing the antigen to which the assay is directed. The user might take a sample of the urine with a dropper supplied in the kit and may add a few droplets to vial 2. The contents of vial 2 would be immediately transferred to vial 1 and the solution therein allowed to incubate for a prescribed period at room temperature. The user could thereupon discard the liquid contents of vial 1 and rinse the vial several times with cold tap water. The contents of vial 4 could then be mixed with those of vial 3 to form an activated chromogenic solution in vial 3. The user could then transfer this solution to vial 1, wait another prescribed period of time, and then observe whether the contents of vial 1 have developed color, thus determining the presence of the antigen being assayed.
These assays described herein are advantageously implemented using room temperature incubations. They have been successfully conducted at temperatures in the range 15� C. to less than about 37� C. for all steps in the assay including the incubation of first antibody to effect adsorption of this antibody to a solid surface. The preferred temperature range for all steps in the assay is 15� C.-28� C.
Although the enzyme immunoassay techniques of the invention have been illustrated in the foregoing detailed description in the context of certain specific enzyme linked immunosorbent assays, it should be appreciated that they may be extended to a variety of enzyme immunoassays. Accordingly, the invention is not intended to be limited to the specific embodiments or examples set forth in the specification, but rather is defined by the claims and equivalents thereof.
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Trail Talk: With the Washington Trails Association
From hiking our beloved Northwest trails to maintaining them, Trail Talk delves into issues that impact our trails and how we enjoy them.
Monthly Archive for December, 2007
Forest Service budget in jeopardy
For three of the past five winters, unusually massive storms have wreaked havoc in Washington's backcountry, washing out roads, bridges, and trails. Most local hikers will remember that Mount Rainier National Park Read More]
Winter hiking / snowshoeing safety
Snowshoes are flying off the shelves at local outdoor stores, and I'm guessing many local hikers will be unwrapping shiny new snowshoes and poles for the holidays. I often hear that "if you can walk, you can snowshoe," and the g [Read More]


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latest posts
Mayor Gavin Newsom at SF Green Festival last Saturday. It's the largest green festival in the country. Mayor Newsom is a long time vegetarian.
The hall was packed for my talk on raw food desserts, nutrition, and overall well being. I demoed my famous Chocolate Raspberry Ganache Cake and Spiced Blueberry Cobbler, recipes from "Ani's Raw Food Desserts". There was a feeding frenzy over the sample tastes of the desserts, and they were delicious!
Read more about the SF Green Fest on VegNews Magazine's blog.
Matthew Kenney's done it again! This time in Oaklahoma City with his partner Dara Prentice. This amazing duo has opened the 1st accredited Culinary Academy for Raw Living Foods, along with a restaurant where you can enjoy Matthew's amazing food.
Join me, Matthew, Dara, and other folks from our community on Oct 3, 2009 in OKC at 105Degrees for a day of talks, demos, dinner, and celebration!
•Philip McCluskey (weight loss)
Read the article in the Asian Reporter on my talk and book signing for Ani's Raw Food Desserts at Powell's Books in Portland, OR last Sunday evening. It was a great turnout, and I was super happy to have so much support from the community in Portland. It was also 90 degrees and gorgeous that day too.
Mango Sorbet-Macaroon Tartlets
•2 cups shredded coconut
•2 cups chopped frozen mangoes
To make the tartlet shells, line 4 small tartlet or brioche cups with plastic wrap. Scoop about 2 tablespoons of the crust into each. Using your fingertips, firmly press the crust into the tartlet cup, leaving a cavity in the center to hold the sorbet. Place in the freezer to firm up for at least 30 minutes or more.
To make the mango sorbet, combine the mangoes, water, and agave syrup in a high-speed blender and blend until smooth. Serve immediately. Or, for an icier, more solid texture, scoop into a container and place in the freezer for an hour or more.
To make the mango sorbet in an ice cream maker: Chill the mixture in the freezer for an hour or so, then scoop into the ice cream maker and follow the manufacturer's instructions.
To serve, scoop 1 tablespoon mango sorbet into each tartlet shell and serve immediately.
The tartlet shells will keep in the freezer for many weeks. The assembled tartlets are best enjoyed immediately, but will keep in the freezer for several weeks. Thaw for 5 to 10 minutes before eating.
– Recipe from Ani's Raw Food Desserts
Check out photos from the wonderful book launch party VegNews Magazine threw for Ani's Raw Food Desserts. It was the best party ever, and people drove for hours to be there. These 2 lovelies had driven up from LA to SF to be at the party with me. How lucky am I? I spoke for about 30 minutes on how I live eco green, and tips for eco entertaining.
BUY NOW: Ani's Gourmet Ready Mix Superfood Chocolate Kit
I've partnered with Superfood leaders Essential Living Foods to bring you the world's first ever raw food Ready Mixes.
It can be expensive to buy pounds of ingredients all at once, so I've created my Ready Mixes which offer pre-measured, smaller quantities of ingredients to make just one batch. The kits are still being tweaked and perfected, but for now, you can get them online only.
Wheat and dairy-free, gluten and refined sugar-free, organic, all superfood ingredients. Eating these desserts can help you loose weight while looking and feeling great!
My proprietary, Award-Winning Chocolate Kits are only available here online. Here's the 2 Ready Mixes, you can order direct from Essential Living Foods:
1) Ani's Gourmet Ready Mix Superfood Chocolate Kit
2) Ani's Raspberry Ganache Raw Chocolate Cake Mix
Ani Launches New VitaMix TurboBlend VS
I was invited to the VitaMix headquarters to shoot a 60-minute DVD they'll include in...
Ani's Raw Food Essentials
My new book, Ani's Raw Food Essentials, will be available everywhere May 3rd! I was...
Ani's Recipes in the 2009 Food & Wine Cookbook
Two of my recipes are included in the 2009 Food & Wine Cookbook: my Zucchini Noodles with...
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By tully | "Boredom at Its Boredest" by Michael Tully September 21, 2004 at 7:54AM
Saw "The Machinist" at the Ziegfeld last night, after weaving through the fortress of barricades that were set up around the Sheraton. Something about some moron named "Bush" having a fancy dinner party and smirking with other rich assholes.
As for "The Machinist," I have to say, now that I've had almost twenty-four hours to reflect upon it, it might be a little bit too much "Memento" and a little bit too little "Taxi Driver" for my particular taste buds. I will say that Brad Anderson has clearly elevated his game to a whole new level, which is quite commendable. While I really dug the look and score and feel of the film--and do, in fact, recommend it--it still got a little too "gimmicky" at the end. But this movie comes down to Christian Bale. Holy GOD, man, that fella looked downright sickening! A brave performance, though I don't know if it's gonna earn him any awards.


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Has anyone else noticed the disturbing trend in the fiction book world lately? You know, the one where the author's name is now huge and the title of the book is small. I realize the book industry is in a bit of flux here but this is very strange indeed. Imagine if artists started marketing their work this way. Wouldn't it be strange to see a large panel with a small painting or photograph and a huge name?
OK I get it. Books are different. The goodness is on the inside and the outside is just a package to sell it, but I am talking about a more disturbing trend that has been with art for quite some time. The trend of artists selling work and viewers buying work on name only.
Name recognition, while being a powerful marketing tool, is by no means a judge of quality. For instance, I had a friend once who in the midst of a conversation about photography said the following blanket statement: "I love (insert artist name here). I just love everything he does." The rest of the conversation went a little something like this.
Me: You love (artist's name)?
friend: Absolutley.
me: You know him?
friend: Well, no not personally, but I love his work.
me: Of course but you SAID, you love him.
friend: so what is the difference?
me: It is a huge difference. He is a person and his work is an object or objects created by him. He could be a complete asshole for all you know.
friend: I doubt it. His work is amazing.
You see where this is going right. Such and such artist can do no wrong and all his work is great because HE made it. While I agree, I really liked one of his books, I thought the rest were mediocre and never bought any of them. But I also never confused the work with the person either. I always try and judge art on the merit of the object, or more often, the effect the object has upon me. If the work moves me in some way or changes the way I think about the world around me, or hell even if I just like the color scheme, then I am a fan. Sure in the past, I have picked up books because I recognized the name, but I have never ordered a book site unseen. (Thank you Photo Eye book tease) So I guess in the end of this ramble, my point is this: Buy work because you love IT.


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Kevin Hart's Nerdiness Rescues Get Hard from Homophobia
By Amy Nicholson
By Stephanie Zacharek
By Stephanie Zacharek
By Alan Scherstuhl
By Calum Marsh
Men in Landslide in Force Majeure
By Calum Marsh
Witherspoon Hoboes Through Wild
By Stephanie Zacharek
Interview: Garfield's Jim Davis
By Amy Nicholson
Assaulted over the years by coups and countercoups and restricted periodically by state control, Brazilian filmmakers learned to cloak their messages in symbol and metaphor. As they entertained and indicted, their efforts gained a strong national and international following, illustrated by those films best known in the U.S.: Dona Flor and Her Two Husbands (1978), Pixote (1981), and Bye Bye Brazil (1979) and Xica (1976).
Though economic factors now severely hamper cinema's revitalization efforts, every now and then a stunning film reminds us of the acclaimed Brazilian legacy. Such a work is director Walter Salles' Central Station, which draws on the rich heritage of metaphoric disguise without ever sacrificing the historical specificity of its two protagonists. An older woman and a young boy make an archetypal journey into the interior of the country and the heart, geographical and emotional terrain too seldom explored.
Retired schoolteacher Dora earns a living writing letters in Rio's central rail station. World-weary, cynical about her customers, she takes their letters not to the post office but to her apartment. There, with the advice of her vivacious neighbor Irene, Dora decides whether she'll discard the letters, mail them or put them in a drawer, holding them for a later decision. One of her repeat customers is a mother, soon hit and killed by a bus. When the woman's 9-year-old son, Josue, now homeless, takes up residence in the station, Dora's life and his become inextricably intertwined.
Needing a television, Dora decides to sell Josue to unscrupulous opportunists who market organs. But she rescinds her decision, rescues Josue in a frightening encounter and flees with the boy in search of his long-absent father. Dora and Josue's unusual encounters -- pursuing dead ends, help from and betrayal by a trucker, a religious pilgrimage -- drive the plot, but it's the mythic quest and the rekindling of Dora's dormant compassion that anchor the film firmly in our hearts. Extraordinary performances by the amazing veteran Brazilian stage actress Fernanda Montenegro and by the irresistibly lovable amateur Vinicius de Oliveira give warmth and depth to Central Station's humor and meticulous observation.
When I asked Vinnie, a boy even more captivating in person than in the film, what he hoped the audience learned, he stated the message perfectly: "I hope people learn to be more compassionate, more open to others, and Brazilians more comfortable with our self-image and the image of Brazil as it really is."
Central Station has won the Golden Globe for best foreign film and is a certain contender for the Oscar. More important, it's a film that touches and endorses the kindness we all need to rekindle and to thrive. In an interview conducted in St. Louis with award-winning documentary director Walter Salles, I learned a great deal about Central Station's production and its explicit parallels to contemporary Brazilian history. Perhaps not surprisingly, comparisons to U.S. circumstances readily suggest themselves as well.
Salles says, "We have lived too long in a culture of indifference and impunity, making consumerism more important than anything else. In Brazil, that ignited the desire to find a more humane quality, to redefine what society could be. Even though it's far from ideal, the U.S. has better wealth distribution and a much better legal system. In Brazil, there's no way to avoid seeing blatant inequalities as part of the structure. The struggle for justice is so palpable I felt this had the makings of a film.
"There's also a strong desire to reshape our own identity, which has affected the construction of the characters. For example, Dora is representative of a culture of cynicism. Why doesn't she send some letters? Because she knows impunity reigns and she will not be caught. So she can cavalierly make moral judgments. She pays a price, living a minuscule life and enduring her own solitude, because if you're incapable of sharing, you don't realize the possibilities of friendship.
"On the other hand, Josue represents exactly the opposite -- the possibility of change. And by his fierce desire to find his father, he redefines his future, rebaptizes himself and offers Dora a second chance she never thought she could have. This is the new Brazil for me. Dora represents the old state of things and the kid the possibility of changing that status quo, of refusing it, of shaping a new identity. The father, then, is a hypothetical father because maybe we can install a new identity in our country.
"The truck driver represents something else happening continuously in Brazil. Due to their solitude, an increasing number of people are drawn to evangelist churches like you have here. But in Brazil, they have transcended religious territory to become a social problem. They specify your obligation to give them 10 percent of your income to save your soul. In a society where so many people are abandoned, many pay that to be part of a group and to find any solace. But in doing so, they refuse other possibilities, including sexual ones. The truck driver, as gentle as he is, has that limit. By contrast, as Dora grows closer and closer to Josue, she gets more feminine. At 67, she realizes she can have a desire for a man. We worked with subtlety to avoid any exploitation.
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Alex Van Halen
View Single Post
Posts: n/a
Re: Alex Van Halen
Originally Posted by
Before David Lee Roth joined the brothers, he was in Red Ball Jet (horrible, just bloody horrible band) in 1970. When he joined Genesis (Van Halen), they had a different bass player (Mark Stone). The boys changed the name to Mammoth ('cause the English band Genesis was breaking). Then another name change to Space Brothers (and they briefly had a keyboard player). Name change back to Mammoth (Mark Stone left, Mike Anthony entered the picture). Name change to Van Halen around 1974. All thru those years they were a cover band. Did a few Budgie songs. Captain Beyond. Lots of Black Sabbath. I don't remember them ever doing any Leppelin covers, and I never thought Alex to have a Bonham influence. Heavily influenced by Ray Phillips and Pete Boot (both Budgie drummers). And Frank Beard.
dude what are you talking about heres another fine exsample of someone opening there mouth and nothing coming out. alex van halen 3 main influances were keith moon, ginger baker and buddy ritch he was NOT a big fan of john bonham in almost 30 years i hered him mention bonham twice.
please you people need to check out other sites and see bonham is'nt much most drummers find him good a best and overrated. dont take my word for it look arround and see for you self.


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It's a bit early for Obama to be a "lame duck President," but clearly that's his situation now.
He's failing to lead. His most recent appointees (Hagel and Kerry) seem to bumble along. The current "solution" to the WMD situatiion in Syria seems to have been a Kerry brainfart which has put Vladimir Putin in charge of American foreign policy.
He seems insular, seems to want to go on his own without interfacing with Congress, even members of his own party.
He seems lost. I almost wonder if he's starting to talk to the paintings of past Presidents, seeking their advice much as Nixon is alleged to have done.
I'm starting to wish the choice had been between Hillary Clinton and John McCain/Mitt Romney. I can't see her in the situation Obama is in.
I was proud that we elected a black President, and in no way will I vote for a Republican for President without a fundamental transformation of their philosophy, but I must say I'm depressed.
Anyone else with me?
Views: 1121
Simple, follow the money and look at the cronism between corporations and Federal Goverment agencies. Like the revolving door between the FDA and Monsanto.
Mark Twain said. "If voting worked they would not let us do it."
This will better explain my position.
I think he actually meant the things he said in his first campaign and really was idealistic. I feel like once presidents get into office, they eventually get worn down by the institution and its day-to-day realities. They get to be at the hub of the decision-making apparatus, but every decision has so many consequences that they feel constant pressure to make mundane practical choices rather than idealistic ones. The weight of history, the wrestling with Congress and lobbying groups, the nonstop schedule, the reality of having to make life-and-death decisions...I think most presidents probably feel trapped and obligated, rather than powerful.
Along the line of what you are saying, I think Presidents can say what they like while running for office, but when the sit down at that Oval Office desk and review the facts and talk to the experts their decisions start to resemble the decisions made by their predecessor. Now in his second term and unable to be elected to a third, Obama's decisions cannot be said to be influenced by lobbyists or promises of work after he leaves office. Face it: Obama will never be out of work or out of money-making opportunities. Offering him a job to influence a decision would simply insult him.
Rather than blaming Obama for supposedly not following through on his promises, people should recognize the signs of a man who is beginning to realize how little he has been able to do, and that mostly due to Republican obstructionism.
I do think he tried, but the GOP, combined with his insularity and other personal shortcomings, kept him from doing all he could possibly do.
Let's hope that the Affordable Care Act, survives. I think it will once people start to benefit from it (or become dependent upon it), it probably will.,
It seems like Americans of a democratic disposition are a bit over yourself fawning over your affirmative action black jesus, and have realized that experience may actually be an important qualification for a president. I guess it's similar to how (some) of your brethren of republican orientation found out the same after the reelection of Bush the younger.
Since 9/11 it has seemed as if the US has really been bumbling along, being pushed by events instead of shaping them. Syria is merely the latest in a decade and a half of US foreign policy mistakes; FP being the policy area in which the president enjoys great latitude. I hope you guys soon find your way again, though until such time I will gleefully indulge in a bit of schadenfreude. I believe it was Leonard Cohen which remarked that whatever one thinks of America you won't like what follows it, and it's a sentiment I subscribe to.


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Under increasing pressure over its longstanding ban on gays, the Boy Scouts of America is proposing to lift its ban for youth members, Fox News reports. The Scouts plan to continue to exclude gays as adult leaders, and announced Friday they would submit this proposal to the approximate 1,400 voting members of its National Council at a May 20th meeting in Texas. Earlier, the BSA indicated it might give local Scout units the option of admitting gays as both youth members and adult leaders, or continuing to exclude them. However, they changed course on Friday due in part to surveys sent out this year to members of the scouting community. Gay –rights groups oppose the ban, some churches and conservative groups want it maintained.
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Danny Devito Reportedly Cheated On Wife With Movie Extra
Although it came as a surprise to most of us, Danny DeVito's shocking split from former wife Rhea Perlam was something to be expected due to his reputation with the ladies.
Throughout the early 90s, while working on the set of DeVito's movie, a production crew member has made staggering claims that the actor had an affair with a young blonde extra and the two would frequently get intimate in his limousine.
Danny had supposedly made several empty promises to the naive actress, claiming he could easily make her a house hold name in the industry.
"It was kind of pathetic because she was very young and beautiful and obviously just having sex with him in hopes that he'd make her a star," the insider claims.
"Danny would have his limo driver drive them all over the city and they'd canoodle in the back before going back to his room together."
Ashton Tyler | Elite.
Photo Credit: Michael Buckner/Getty Images


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What type of beauty product do you think is worth splurging on?
What type of beauty product do you think is worth splurging on? Lip balm? Skincare? Hair products? Eyeshadow?
Anything you'll actually wear and wear often can be worth splurging on. Something you might wear once or twice is not. I would also say basics like makeup removers, skin care, foundation, and primers are all worth investing in if higher priced products work better for you.
Thanks to Ella for today's question! Do you have a question idea? Submit yours here.
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Thursday, February 16, 2012
OSU-Tulsa events celebrate Black History Month
Oklahoma State University-Tulsa will host a pair of events this February to celebrate Black History Month.
"Black History Month is a time for us to promote, educate and celebrate the great achievements of African Americans who have made a positive difference for all of us," said Nekki Reagan-Neely, director of OSU-Tulsa's Student Success Center.
Patrick Chalfant and Dr. Wade McCoy will discuss the life of their former professor and mentor, Dr. Henry Kirkland Jr., at a presentation on Thursday, Feb. 23 at 6 p.m. in North Hall 150. Chalfant and McCoy wrote A Rainbow in the Dark based on the life of Kirkland, who overcame poverty and racism in 1940s Oklahoma to become a celebrated educator and the first African American professor at Southwestern Oklahoma State University.
The OSU-Tulsa Minority Support Services/Campus Life will host "A Taste of Soul" on Wednesday, Feb. 29. The event will begin at 3:30 p.m. in the North Hall Lobby. Participants can sample various soul food cuisines, including fried chicken, macaroni and cheese, candied yams, collard greens, corn bread and peach cobbler.


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Photos and Review by Oyster.com Investigators
On a somewhat busy road dotted with hotels, and not much is within walking distance except for some low-key attractions, like the estuary
The hotel is on the outskirts of San Jose del Cabo, and the immediate area doesn't offer too much within walking distance -- the road it's located on is mostly dotted with various hotels. The area, much more low-key than downtown Cabo San Lucas or even downtown San Jose del Cabo, offers a more relaxed pace. For example, one of the closest attractions within walking distance is the San Jose Estuary, which is a great spot for kayaking, walking, and bird watching.
Close to the party-hearty area of Cabos San Lucas, San Jose del Cabo is the quainter, historic answer to a beach vacation in Los Cabos. The waters are rough and there's less of a nightlife, but the area retains its Mexican flair, and features numerous restaurants and galleries. It is also close to the airport.
The atmosphere at the Grand Mayan is peaceful (aside from pushy timeshare sellers): The massive pool looks out onto a pristine beach, the Brio Spa has soothing decor, and the chic and spacious rooms feature private balconies and bathrooms with Jacuzzi tubs and walk-in showers. In the quiet area of San Jose del Cabo (as opposed to party-hearty Cabo San Lucas), the hotel is within walking distance of town and caters toward couples with its sexy open bathrooms and upscale restaurants (although there are some mixed reviews on the food). Drawbacks, however, are fairly significant -- the pool temperature is often chilly, and the beach is riddled with jellyfish.
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text: Revenue Has your pharmacy taken a hit in sales? VETERINARY ECONOMICS: Aug 01, 2015 By dvm360.com staff Data from Benchmarks 2015: A Study of Well-Managed Practices show that 74 percent of practices have seen sales drop in the last two years. Stop selling in the exam room—here's how VETERINARY ECONOMICS: Jun 29, 2015 By Marty Becker, DVM Get veterinary clients to buy in to a recommended brand of pet insurance so you can focus on care. Pinpointing veterinary acupuncture VETERINARY ECONOMICS: Jun 29, 2015 By Matthew Kenwright, Assistant Content Specialist Adopting acupuncture appeals to more clients and gives practices another tool in the toolbox. 6 reasons why the proposed Fairness to Pet Owners Act fails veterinary patients, practices VETERINARY ECONOMICS: Jun 25, 2015 By Bash Halow, LVT, CVPM The legislation is a solution to a nonexistent problem, veterinary business consultant writes in an open letter to Congress. How I learned to stop worrying and love the heartworm test VETERINARY ECONOMICS: Jun 18, 2015 By Veronica Hanley This Practice Manager of the Year nominee sold her practice owners a plan for new vaccines and testing. The result? Practice revenue. Q&A: Should veterinary practices be open Saturday? VETERINARY ECONOMICS: Jun 02, 2015 By dvm360.com staff Veterinary Economics Editorial Advisory Board members are mixed on weekend's potential. Two practices shine light on laser therapy in the veterinary business model VETERINARY ECONOMICS: Jun 01, 2015 By Rachael Zimlich Billing it as a standard in your practice is among the takeaways of success stories. Use this simple trick to stop missed charges at your veterinary hospital VETERINARY ECONOMICS: May 01, 2015 By James W. Randolph, DVM This veterinary practice sets an eye-catching number as the default dollar amount for procedure codes, rather than zero. I don't care about this data ... maybe you shouldn't either DVM360 MAGAZINE: Apr 01, 2015 By Mike Paul, DVM Two surveys of national veterinary fees and charges may contradict each other. Here's what you should really focus on. Data Center: Levels of veterinary care clients want vs. what they'll pay for VETERINARY ECONOMICS: Mar 01, 2015 By dvm360.com staff Managers were asked to give their perceptions of what their clients wanted. 10 revenue-boosting tactics to pay staff more VETERINARY ECONOMICS: Feb 01, 2015 By Ernest E. Ward Jr., DVM If your team members deserve better compensation—and if you want to keep the best of the best, they do—you need to boost your revenue. Here's how to make it happen. Plus, data from 2015 Firstline Career Path Study. Does new data show veterinary prices dropping? DVM360 MAGAZINE: Jan 30, 2015 By Brendan Howard, Business Channel Director Uncle Sam says post-recession prices are up 15 percent, but new research from VPI-Nationwide and Purdue University hints at stagnation. Why your veterinary practice isn't covering its costs VETERINARY ECONOMICS: Nov 20, 2014 By dvm360.com staff Caution: Price raising talk ahead CATalyst gets shelter pets into veterinary clinics DVM360 MAGAZINE: Nov 01, 2014 By Sarah A. Moser One year in, an innovative program sees some success at connecting new pet adopters with local veterinarians. Why all the fuss about low-cost veterinary clinics? DVM360 MAGAZINE: Nov 01, 2014 By Joan Freesh, MS, DVM Veterinarians agree that spaying and neutering pets is a good thing. Stop being so concerned about who provides the service. 1 2 3 4 5 ... 39 Next » Last » Hot topics on dvm360Vetcetera: The Fairness to Pet Owners Act is back Jul 24, 2015Fear-Free underwater treadmill session for therapy and analysis Jul 22, 201522 stress-hacks that will change your lifeMay 27, 2015VetConfessionals: Veterinary secrets, shared at CVC 2015 May 17, 2015Top 5 signs it's time to quit your job in veterinary medicine May 08, 2015|More|


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Details and Shopping
Customer Favorite
- FP Exclusive :
- Only sold thru Free People
Heathered and casual sleeveless dress with stretchy fitted waist with drawstring ties at the front of waist. Slight hi-low hem. Elastic all around the top hem.
*55% Cotton, 35% Polyester, 10% Rayon
*Machine Wash Cold
*Machine Wash Cold
Measurements for Small:Length: 38"
Bust (all around - relaxed): 29"
Waist (all around - relaxed): 28"
Rated 3.3 out of 5Â by 3 reviewers.
Rated 5 out of 5Â by s_a_w so good. love, love, love this dress. probably not worth the original price but i bought the mocha color on sale and have gotten so many compliments! i've worn it to the beach, running errands, even to a casual bar at night. perfect for just throwing on and heading out the door. just ordered the berry color and would definitely buy any other color available! 7.9.2013
Rated 1 out of 5Â by denisehausen yuck! This dress is theee worst fit I can imagine. I do have a long torso but I am only 5'6 and the waist line on this dress went up way high. I did get the extra small but the fit was just unflattering. Plus it is paper thin-see thru! Sent back. 5.24.2013
Rated 4 out of 5Â by anniej great summer piece!! Love this dress, love the fit, love the color, ONLY thing I'm bummed about is that it's a tad see-thru.. Who wants to wear a slip in the summer?? I will still wear it all the time around the house and to the beach.. Hoping the cotton thickens up when I wash it. 5.17.2013
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Check out our ideas for creative ways to have fun at the cabin.
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Khloe Kardashian Throws a Fit Over Joan Rivers' Prank
Scott Disick Talks Baby Number 4 With Kourtney Kardashian
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Meet The Newest Member of The Duggar Clan
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BURRILLVILLE — History has been extremely good to the Burrillville/Ponaganset co-op team when it comes to playing Barrington's East Bay co-op squad.
Even since Barrington joined the girls' hockey varsity ranks eight years ago, the Broncos have had the Eagles' number. They own a 12-2 record against them, and a five-game win streak that has seen the Eagles strengthen their numbers by adding Mount Hope and Portsmouth to its co-op squad, yet the Broncos still win each time by five or more goals.
Last Monday night, when the Broncos skated off their Levy Arena ice with a 4-0 win over North Smithfield in Game 3 of their tough best-of-three Division I semifinal-round series, word quickly spread throughout the rink that their opposition in this weekend's title series would be the East Bay team.
Plenty of smiles broke out among the Burrillville fans, and talk of the program's first state championship was alive and well in the arena's lobby.
After all, it was only eight days earlier when the Broncos routed the Eagles by a 6-1 score in their regular-season finale. How difficult could a best-of-three series against them be?
First-year Burrillville head coach Marc Brissette gave the answer to that question before his team's practice on Tuesday afternoon -- very difficult.
"That score was not indicative of the game," he admitted. "I think Barrington has a very talented team. They have a couple of freshmen who were among the league leaders in scoring, they have a couple of really good defensemen, and their goalie is good.
"We definitely have our work cut out for us, so we definitely aren't looking past that team whatsoever."
The Broncos, who will be making their first state title appearance at Providence College's Schneider Arena since their inaugural season of 2002-03, and the Eagles, who are back at PC for the first time since winning the then-called Division II crown in 2007, will kick off their series on Saturday at 3:30 p.m.
Game 2 of the series will be held on Sunday at 1 p.m., and if a third and deciding game is needed, it will be held on Tuesday at either 6 or 7:30 p.m.
The Eagles skate into this series with an 12-6-1 record, their second-best mark since their glorious 2006-07 season, while the Broncos head into Providence with a 10-9 mark.
Someone who is unfamiliar with girls' hockey in Rhode Island and looking at both records would automatically give the Eagles the nod to claim this series, but a deeper glance at the records would most likely change their mind.
The Broncos played in the extra-competitive 'Emma' division against the likes of longtime parochial powers Mount St. Charles, La Salle, and Bay View and faced each team twice, while the Eagles played in the seven-team public school 'DaCosta' division and contested their peers twice.
But when it came to the crossover games, the Broncos played the 'DaCosta' squads only once, while the Eagles took on the Broncos and the parochial teams once.
The Broncos ended up with a 1-8 record against their parochial competition, but Brissette only saw the positives when he talked about his team's challenging schedule.
"Playing in our division was definitely beneficial," noted Brissette. "It helped us because it made us that much stronger and that much better. I'd rather play that type of competition because the games are close and the competition makes you better, as opposed to playing down a division and sometimes beating some teams 10-0.
"But I told the girls, 'Don't believe for a second that just because Barrington played in the 'DeCosta' division, that they're not a good team,'" added Brissette. "They're very well-coached, and like I said, they have some talent on that team."
And most of the talent the Eagles have is young. They feature the league's leading scorer in freshman Meghan Miller (26 goals, 18 assists) and another offensive threat in freshman Kelly Kraunelis (12 goals, 12 assists).
Freshmen Leah Hoder and Danielle Makucevich are two of their top defensemen, and the goalie, sophomore Sophie Faxon, the daughter of pro golfer Brad Faxon, is also good.
The Eagles only have three seniors, but they are battle-tested veterans and their captains.
Chelsea Larisa is fourth in the state in scoring (15 goals, 20 assists) and a physical player, Kaitlyn Mullen leads the second line, and Emily Carlson is another standout defenseman.
The Broncos are also very low on seniors, having just two, and one of them, team captain and center Kelsey Koprusak, will be capping one of the greatest careers in the program's history this weekend.
Koprusak, who leads the Broncos with 15 goals and six assists, has established herself as one of the state's all-time leading scorers with 59 goals and 66 assists.
"She's such a terrific kid," said Brissette. "She was the Student-Athlete of the Month for January, and she's looking to play hockey in college. Right now, she's looking at a couple of different schools. She already got accepted to Sacred Heart and she's looking at Salve Regina."
And while Koprusak's offense and all-around play have benefited the Broncos this season, so has her leadership abilities, something Brissette says she takes great pride in.
"She knows when to put the foot down and she knows when to back off, and she's really taken the girls on the past few weeks," offered Brissettte. "Between our assistant captain, Jackie Keable, and Kelsey, they've done a terrific job at being leaders in the locker room and on the ice."
Like the Eagles, the Broncos also have an outstanding freshman class that features Emily Cardon, who is second on the team in scoring (10 goals, four assists), playoff hero Maggie Reid, and Teigan Palazini up front, and Tiara Bianco on defense.
Keable, junior Sam Roberts, and sophomores Michaela Smith and Christina Bruno have also played well on defense, and senior forward Audrey Jerome and junior goalie Britney Bebeau, who boasts a 1.66 goals-against-average and .922 save percentage, are also worthy contributors.
During his talk with the media on Tuesday afternoon, Brissette did reveal something that not many folks around the girls' hockey circuit know -- he tends to get emotional and let his tears flow after big games, win or lose, just like former NFL head coach Dick Vermeil did on occasion when he was in charge of the Philadelphia Eagles, St. Louis Rams, and Kansas City Chiefs.
"I'm a very emotional person that cries at everything, and the girls are always goofing on me because of that," admitted Brissette. "When we beat North Smithfield [on Monday night], that was kind of emotional, and in the locker room, the girls said, 'Coach, you're crying again,' and I told them, 'There's nothing wrong with crying.'
"When we talked to them after the game, I said, 'You know, some of you may never get this chance again.' It's been almost 10 years since this team has played for a championship. I told them, 'Relish the moment, enjoy it, and then we're going back to work,' so hopefully we'll come out on the winning end when this is all said and done."
[1] http://www.woonsocketcall.com/sites/default/files/030112BurrGirlsKopJerome.jpg
[2] mailto:firstname.lastname@example.com


text: Prev | Next Purchase this photo Billy Summers/The Putnam Herald Makayla Schoonover, 3, bats her bright eyes at the camera on Saturday, Sept. 22, 2012, during the Creek Cabin Antiques Festival at the Winfield Riding Stables. Gallery: Putnam in Pictures, Friday, Sept. 28 Sep. 28, 2012 @ 04:52 PM 2012/The Herald-Dispatch Did you miss something in Putnam County this week? Chances are, one of our photographers was there. ()


text:
RESEARCH CENTER for DELMARVA
HISTORY and CULTURE
at Salisbury
Map & Directions
Wayne and Power Streets
Salisbury, MD 21804
(410) 543-6000
Visiting Hours
Monday 10 a.m. - 8 p.m. Tuesday-Friday 10 a.m. - 4 p.m.
Scope and Content Note
Organization and Arrangement
Index Terms
Related Material
Administrative Information
Container List
Costen House in Pocomoke City was owned by the first mayor
of the city, Dr. Issac T. Costen. The house was built
sometime prior to his being elected mayor in the early
1880's. The Costen House was placed on the National Register
of Historical Sites in 1974.
Return to the Table of Contents
This collection
contains a pamphlet on the Historic Costen House at Pocomoke
City, Maryland. It was written and published by Walter R.
Schlining in 1983. It contains a brief history of the house,
along with various photos of the house and the surrounding
The collection is open for
The collection is the physical
property of the Edward H. Nabb Research Center for Delmarva
History and Culture. Nabb Research Center holds literary rights only
for material created by Nabb Center personnel working on official
behalf of the Nabb Center, or for material which was given to the
Nabb Center with such rights specifically assigned. For all other
material, literary rights, including copyright, belong to the
authors or their legal heirs and assigns. Researchers are
responsible for obtaining permission from rights holders for
publication or other purposes that exceed fair use.
These records are indexed under the following headings in
the Edward H. Nabb Research Center for Delmarva History and
Culture catalog. Researchers wishing to find
related materials should search the catalog under these index
Costen House
Collection, Edward H. Nabb Research Center for Delmarva
History and Culture, Salisbury University.
1 | 1 1984
pamphlet on the historic Costen House in Pocomoke City, MD
Copyright & Fair Use
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East Campus Room 190
| Salisbury, Maryland 21801 | 410.543.6312


text:
- El Corredor Nocturno
- (2009)
Runtime: 98 min
Rating: 5.5
Status: Released
"El Corredor Nocturno" is a new foreign and thriller movie that was released in 2009.
Eduardo, manager of an insurance company, is under a lot of stress and that's why he runs, especially when he is about to explode. One day, returning from an unsuccessful business trip, he meets a mysterious man at the airport who presents himself as a friend and benefactor and encourages Eduardo to change his life and be free
Best actors like Erica Rivas, Leonardo Sbaraglia and Miguel Angel Sola starred in El Corredor Nocturno.
|Images ❖
|Cast ❖
Erica Rivas
|as Clara
Leonardo Sbaraglia
|as Eduardo López
Miguel Angel Sola


text:
2 Line
2 Line Phones For Home Or Office - A
2 Line Cordless
Phone | 2 Line
Phone System | 2 Line Answering Machine
| Panasonic 2 Line
You can use 2 line phones in your business and home with your single existing phone jack.
A 2 line phone can enable someone working from home to have a separate business line,
either for voice, a fax or a modem. It is also a very useful thing for most families,
specially those that have teenage children who use the phone a lot, and makes it possible for a parent to
dial out with the second line regardless. In fact, many households now have two phone numbers listed—one for the
adults and one for the children, making for a more harmonious family life, not to mention safety.
Your existing wall jack is most likely already configured for two lines and you could have two active lines
even without a dedicated 2-line phone but you would need a two-line splitter that would plug into the phone
jack and "split" the lines into two single phone lines--one phone for Line One and a second phone for
Line Two. It is much simpler to just have one phone and use it for either line.
Besides its practical uses in the home, the 2 line phone is also used by small businesses where
there is a need for someone to handle incoming calls at all times while leaving a line open for calling out. It is
possible to have a full blown business phone system at almost no cost with the right equipment.
There is another compelling reason to consider these phones: the IRS may allow you to deduct your home
office expenses that are used specifically for business and a second line that is dedicated to business
only will help you document this deduction very efficiently. Of course, you need to check with your tax
professional for more details and suitability for your particular situation.
Another common use of the two line phone that you may be familiar with is used extensively in
hotels so you can plug your laptop into a socket to use a high speed modem while also using the voice phone
over the same wiring without any problems.
To recap, here are some of the advantages to a two-line phone:
being able to talk to someone and be connected to the
Internet simultaneously with the same phone
have two people be on the phone talking to different people at
the same time
be able to have a three-way conference call with two lines
locally connecting to one outside number
having a visual que in the form of an LED that lets you
know whether you are on line or not.
How to set up a 2 line phone systemAs mentioned before, your phone line is probably already
compatible with a 2-line system. If you find that your wiring is not compatible, you can purchase a 2 line
phone jack, adapter or splitter in any phone store. Setting up the wiring is not too complicated either--your main
phone cable is actually made up of 4 sub-cables (or wires) and each pair can provide you with one phone
line. At the main phone box where the phone company's line enters your home, the splitter will lead two
separate lines into your home--Line 1 and Line 2. You can choose where these lines go.
Perhaps you want Line 1 to be the "family" line and Line 2 to be your "business" line. That's just one way to
do it. Another would be to have Line 1 for parents and Line 2 for the children.
You will most likely be dealing with a 2 line cordless phone as this is the standard nowadays but
if it does not work well in your situation for any reason, including wireless interference in your area
or other reception problem, you can use a corded 2 line phone just as easily. Most businesses have both.
Here is a short representative list of some of the more popular 2 line phones at time of writing:
- Panasonic 2-line phone KX-TG9382T DECT 6.0 Expandable Digital Cordless Answering System
w/ 2 Handsets.
- Uniden 2 line telephone DECT 4086 DECT 6.0 Cordless Digital Answering System
- VTech 20-2481 2.4 GHz DSS 2-Line Expandable Cordless Phone w/Digital Answering
- GE Two Line phone 28165FE1 Dect 6.0 Single Handset w/ Answering System.
Bottom line is that in this day and age of the Internet and the home office where more people are working
remotely from home, it makes a lot of sense to take advantage of the capabilities and efficiencies that you
can get with a 2 line phone.
2 Line Phone
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text:
Contacts: Gregg Mazzola
Director of Communications
Southern New Hampshire University
Cell Phone Collections for Soldiers at SNHU
Cell Phones for Soldiers hopes to turn old cell phones into more than 12 million minutes of prepaid calling cards for U.S. troops stationed overseas in 2008. Faculty, staff and students can help soldiers phone home by placing old cell phones and chargers into the drop box located next to the Info Booth in the Student Center. SNHU then forwards the phones to CPFS founders Robbie and Brittany Berquist.
The Bergquists, teenagers from Norwell, Mass., started CPFS with $21 of their own money. Since then, their registered 501c3 nonprofit organization has raised almost $1 million in donations and distributed more than 400,000 prepaid calling cards to soldiers serving overseas.
"Cell Phones for Soldiers started as a small way to show our family's appreciation for the men and women who have sacrificed the day-to-day contact with their own families to serve in the U.S. armed forces," says the teens' father, Bob Bergquist. "Over the past few years, we have been overwhelmed by the generosity of others. But we have also seen the need to support our troops continue to grow as more troops are sent overseas for longer assignments."
The phones are sent to ReCellular, which pays CPFS for each donated phone. Each phone provides one hour of talk time to soldiers abroad. Please visit http://www.cellphonesforsoldiers.com/ or call 603.645.9668 for more information.


text:
Tasty Tuesday: Baked Oatmeal (with chocolate chips)
I always thought I didn't like baked oatmeal. I mean I knew it was supposed to be a healthy, cheap food but somehow it just always seemed mushy and unappetizing. That is until a couple of weeks ago when I tasted this recipe at the brunch my mom's group had. I had actually passed it by, but one of the ladies sitting at my table kept mentioning how good it was and so I went and got some to try for myself. And it was good! So good in fact that I had seconds and then tracked down the recipe too! o
I think what makes it a winner for me is the fact that it doesn't just use all regular oatmeal but instead it adds in rolled oats too which gives it a great texture. That and the fact that it has chocolate chips. Oh yum! What isn't better with a few chocolate chips? Of course if you disagree, you can always leave them out. But I'd recommend not doing that. I'm pretty sure you'll wish you hadn't. Anyway…on to the recipe.
Baked Oatmeal
1 1/2 c. quick cooking oats
1 1/2 c. rolled oats
1 c. brown sugar
2 tsp. baking powder
1 tsp. cinnamon
1 tsp. salt
1 c. milk
1/2 c. butter melted
2 eggs, beaten
1/4-1/2 c. semi-sweet chocolate chips (optional)
In a mixing bowl, combine all ingredients. Spoon into a greased 9″ in. baking dish (or any similar sized baking dish). Bake at 350 degrees for 30-35 minutes or until edges are lightly browned and center is set. Yield: 6-8 servings
OTHER NOTES: You can use 3 c. quick cooking oats instead of half quick cooking, half rolled oats. Feel free to try other add-ins such as apples, blueberries or bananas. Some people like to eat baked oatmeal without any add-ins and then top it with fruit. I also know people that love eating it with milk dumped over it. Obviously it's quite versatile! Oh, and supposedly baked oatmeal freezes really well. You can either freeze it unbaked, thaw it in the fridge and then bake it or you can bake it, freeze it and then just reheat it. This works really well if you put it in individual portions to freeze.
For other recipe ideas, check out Balancing Beauty and Bedlam's Tasty Tuesday and Blessed with Grace's Tempt my Tummy Tuesday.Add a Comment
Tags: | Categories: Tasty Tuesday, The Thrifty Frugal Mom
Must Read
by Susan
On May 8, 2012 at 7:16 am
We do like baked oatmeal (even though Joe doesn't like to make it soggy with milk– I think that is what makes it good!) Anyway, I make my granola with the 2 oatmeals mixed together so I make everything that way because it is already mixed. (cookies, cake, etc.) It does just give an extra crunch!
by Susan
On May 8, 2012 at 7:16 am
Forgot to add that I want to try it with the chocolate chips!! Thanks for the recipe!
by Lydia Beiler
On May 8, 2012 at 8:01 am
Susan, funny how you like it soggy and that is what I think makes it gross. I love your idea of mixing the 2 oatmeals for other stuff too. I'm going to have to try that! I can imagine it adds something.
On May 8, 2012 at 9:35 am
We LOVE baked oatmeal, but I've never tried it with chocolate chips. I know my kids would go for that! I'll have to try it sometime. I am over from the Beauty and Bedlam link up. I blogged my Tasty Tuesday Banana Bread recipe at: http://livetobeinspired.blogspot.com/2012/05/banana-nut-bread.html
by Hanunyah
On May 8, 2012 at 5:05 pm
Baked oatmeal has been an easy favorite at our house for quite a while! Yum!
by Lydia Beiler
On May 8, 2012 at 7:03 pm
Taralyn, I'm sure your kids will love it!
Hanunyah, thanks for stopping by and taking the time to comment!
by Menu Plan: What Our Family of 4 Eats on $200/mo. | The Thrifty Frugal Mom
On November 2, 2013 at 8:44 am
[...] eggs; sausage; homemade baked oatmeal with chocolate chips; bagels; fruit slush; orange [...]
by Menu Plan: What Our Family of 4 Eats on $200 a Month (Post #3) | The Thrifty Frugal Mom
On June 27, 2014 at 7:57 am
[...] Bacon and Egg Quiche; Fruit Slush; Baked Oatmeal with Chocolate Chips; Homemade [...]
by Weekly Spending Summary (7/20-7/26) | The Thrifty Frugal Mom
On July 28, 2014 at 8:02 pm
[...] as a side with eggs too. It really helps make a quick easy lunch or dinner. (We tend to eat baked oatmeal or homemade granola for breakfast and enjoy hot breakfast food for our other [...]
by Making the Play with Andrew Luck | GoodyBlog
On September 11, 2014 at 9:33 am
[...] is my most important meal. It's often the meal you play a game on. I make sure I have oatmeal, milk, and fruit. It's the fuel you use to hopefully do your best, so eating right is a big [...]
by Menu Plan: What Our Family of 4 Eats on $200 a Month - Thrifty Frugal Mom
On December 22, 2014 at 8:44 am
[...] eggs; sausage; homemade baked oatmeal with chocolate chips; bagels; fruit slush; orange [...]
by Baked Chocolate Chip Oatmeal + HOT POCKETS® Breakfast Bites, Bite Through Morning Hunger
On April 22, 2015 at 12:48 pm
[...] at 350 degrees for 30-35 minutes. Notes Recipe from The Thrifty Frugal Mom [...]
by Baked Chocolate Chip Oatmeal + HOT POCKETS® Breakfast Bites, Bite Through Morning Hunger | Our Holly Days
On May 13, 2015 at 1:01 pm
[...] at 350 degrees for 30-35 minutes. Notes Recipe from The Thrifty Frugal Mom [...]


text:
CTIA is in full effect and along with that came droves of industry and app/developer related news. Since we couldn't possible feature it all on our front page, we left the questionable ones here, in the Android Overload, for you guys to give the final verdict. If you find a gem, let us know!
- Verizon's 4G LTE "Double Data" promo will end on June 3rd. [DroidLife]
- MasterCard introduces new PayPass Wallet Services. A slap to Google Wallet's face. [Engadget]
- Sony open sources music player and 8 Game extensions for Sony SmartWatch. [Sony]
- More Facebook users visit the site via their smartphones vs computer. [Reuters]
- Google controls which which AT&T devices receive updates? No. [SFGate]
- State of Nevada approves Google's self-driving cars. [Reuters]
- HTC Rezound gets another leaked Ice Cream Sandwich build (3.14.605.5). [AndroidPolice]
- Sony Hayabusa (LT29i) leaked in blurry pics. [PhoneArena]
- US carriers want to move away from subsidies — but it will likely never happen. [WSJ via Reddit]
- Verizon and Color sign exclusive HD video chat deal. [TalkAndroid]
- Angry Birds made Rovio $106 million in 2011. [BGR]
- Google calls for mistrial after jury says Android stole from Java in Oracle trial. [Wired]
Continue reading:


text:
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text:
Jon Theodore
View Single Post
Join Date: Jul 2005
Posts: 2,430
Re: Jon Theodore
Originally Posted by
i gotta say, i don't understand how/why people compare this band to Zepellin. Not familar with Yes so much but Zep? i infact think they've grown ABOVE and beyond the like of those bands. Zep kicks ass but they infact DO have their own sound, different from Zep. It's a shame and makes the one who offered that comment seem shallow and quick to place judgment on a band that does not immediately satisfy one's traditional grounds for musicianship or feeling. Could you please elaborate on those comparisons?
At the risk of coming off as overly defending, their long periods of no playing and guitar effects are part of what make the band. It's a ride, not so much a smiling entertainment clown.
Basically the comparisons to Led Zep are based on various things, but mostly the vocals. The comparisons to Yes are based on pretty much everything else. Grab yourself a copy of "Close to the edge" and tell me the Mars Volta haven't listened to that one obsessively for a few years.
Before I heard TMV a friend of mine mentioned them. I asked what they sounded like. He said "Yes, but with Robert Plant singing". Pretty much sums it up, IMHO. Except the rest of the band occasionally drops into sounding quite zep-ish too. Cicatriz ESP sounds a lot like Achilles Last Stand to me from a rhythm section POV, particularly on Scabdates when they play it faster than usual.
They do occasionally break out something that sounds non-specifically 70s but not directly identifiable as Zep or Yes. But I've yet to hear anything which makes me go "Hey, that sounds like the Mars Volta" when I hear another band doing it.
Also, please don't call me "shallow" because I disagree with you on the originality of the band. It must be noted that of the two major influences I named you appear to only be familiar with one, so I think it's a bit early for you to start throwing mud, no?
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When I was approached by Thymes to design a few unique jewelry pieces to embody the essence of the Jade Matcha, Tiare Monoi and Lotus Santal fragrances, I knew I was in for a big challenge…
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I've wondered about this mostly in relation to games. I expect the gaming segment to be the biggest winners with the new speed and graphics.
It is not that unusual to see games bundle code to handle parallel technologies, and the phone has the memory to store the extra software.
However, I have to point out that if Apple wrote their APIs correctly the software developer should be none the wiser as to which chipset is running the video.
The game just gets the best the device can handle, without having to write special code. This has been the trend in software development and device drivers for a long time, so I don't think Apple would make a 1995 mistake and require developers to be aware of the chipsets involved on any specific phone.
That still leaves the very likely possibility that some games tailored around a 3Gs would run like molasses on a 3G.
At this point the differences are really not that great. Processor speed increments of 100% really don't amount to much.
The OpenGL ES 2.0 presents more of a problem, but this too can easily be handled by turning off some visual special effects in the game, flattening the lighting, reduced rendering quality, etc. And, as I mentioned, if Apple did their homework the game developer won't have to worry about this.
However, the long term accumulation of incremental improvements becomes significant. Some games may have to be restricted to the 3Gs, but more probably about the time Apple kicks the G4 out the door next year, this platform dependency will become acute.
We accept this with our computers, and I see no reason to expect anything different with our phones which are computers.
Radical statement:
Virtually ALL improvements in processor speed since 1995 have been gobbled up by look-and-feel.
The spread sheet does not calculate faster. The report does not print faster. Typing data into computers is not faster.
It just looks better. The games look way better. Gaming is the single biggest beneficiary of processor speed, and the rest largely goes to look-and-feel.
So expect the G4 (or what ever its called) to look REALLY good.
iPhone 3G S and OpenGL ES 2.0 Hardware Accelerated Graphical Goodness Also a Platform Splitter?
This morning we wondered aloud about whether the iPhone 3G S would be splitting Apple's mobile OS X platform. Looks like we weren't the only ones wondering.
Engadget's Chris Ziegler takes a look at the new hardware's PowerVR SGX graphics-core's support for OpenGL ES 2.0, and that led him to this language in Apple's iPhone SDK documentation:
"When designing your OpenGL ES application, the first question you must answer is whether your application will support OpenGL ES 1.1, OpenGL ES 2.0, or both... Your application should target OpenGL ES 1.1 if you want to support all iPhones and iPod touches."
Sure, with 40,000,000 iPhones and iPods touch on the market, many developers will want to stick with OpenGL ES 1.1 support, but like with any generational transition -- even slow ones -- eventually more and more might just want to push the new hardware and see what it can do...
Have something to say about this story? Leave a comment! Need help with something else? Ask in our forums!
Reader comments
iPhone 3G S and OpenGL ES 2.0 Hardware Accelerated Graphical Goodness Also a Platform Splitter?
I've wondered about this mostly in relation to games. I expect the gaming segment to be the biggest winners with the new speed and graphics.


text:
Risk Management and the University Safety Committee have developed a concise poster that lists steps to take in an emergency. The hope is that this poster will sufficiently attention grabbing that it will be noticed by faculty, staff, and students; getting this important information to a wider audience. Feel free to print and distribute and/or post in your department or office!
Click the image below for a larger, printable PDF version of the poster.
Also check out the Earthquake Know What to Do flyer!
Thanks to SMU and TCCD for their assistance in creating this based on their posters!
page last updated 5/26/2015 4:51 PM