text:
I'm new to this giveaway thing. I think it's going well, but my style of announcing giveaways has been all over the place.
With my first two giveaways, I reviewed the books. Then I ran into a snag. I knew the author of the third book, so I just interviewed Deborah Madison instead of reviewing her cookbook, because I knew I couldn't be objective about a friend I met more than 10 years ago.
Now we come to David Lebovitz, another friend I adore, and I have a fourth way to handle this giveaway. I'm going to talk about his video, and a little about the book, and a little about David, one of the nicest, most talented, smartest, funniest and most generous food writers I've ever met. (See, I told you I couldn't be objective.)
One of the dilemmas facing someone with a blog as huge as David's is that it's a challenge for his publisher to reach as many potential buyers for Ready for Dessert: My Best Recipes. After all, his blog has more annual readers than at least two of the top national food magazines. So Ten Speed Press agreed to do a video with a James Beard award-winning documentary filmmaker David had met earlier in France.
"I wanted to do something snappy that wasn't just me talking to the camera, or cooking," explained David. "I wanted to show Paris, too, since that's part of my life and my story. Mark from Flow Films shot the whole thing in HD on his Canon DSLR and we kind of went and just did it. I put a notice on Twitter that anyone who wanted to come taste cookies should show up! Then we also went into some shops, like the chocolate shop of Jacques Genin and a motorcycle shop to get the opinions."
Apparently publishers are doing more of these promo videos. Says Aaron Wehner, publisher of Ten Speed Press, "We are doing videos for most of our lead cooking titles in Fall. The cost can range from $1,000 to $5,000 for something professionally done, depending on length, number of locations etc. We've been doing book trailers for a few years (see Alinea and Peter Reinhardt, among others) but David's came off particularly well." I agree. Like David, the video is warm, fun, and funny.
Now, on to the book itself. It's a big, hardcover, classy looking cookbook, full of dramatic photography and doable recipes. I made the huge Apple-Pear Crisp with Grappa-Soaked Raisins and Polenta Topping. It served 6 at dinner easily and made spectacular leftovers, even when cold. I also made Cranzacs, David's riff on Anzac cookies, a chewy, coconutty oatmeal cookie that my husband assured me will be gone before I get back from IACP's annual conference this coming Saturday night. I'm hoping to make more recipes before I have to give this book away, including his famous Fresh Ginger Cake.
And speaking of giveaways, if you'd like this book, please write a comment below on your favorite dessert. I'll chose a winner by midnight April 26. Contest rules: You must live in the US (Sorry, fellow Canadians). One entry per person. If the winner does not respond within a week, I will choose someone else.
Update: Winner is Elizabeth Greene. Congratulations!
189 Responses to "Cookbook Giveaway: Ready for Dessert"
It's not just your fellow Canadians you are excluding here. The web is worldwide. I read you regularly from New Zealand. I am sure there are many others international readers that are also excluded. I admit to some bitterness because non-US residents are routinely excluded from this kind of stuff. Really rather disappointing, I must admit.
My favorite dessert? Creme brulee. My most memorable dessert was at Aubergine in Whistler, BC. It was five different flavors of creme brulee, and it was just perfect. Got to love cracking the sugar on top and digging into the creamy custard.
While I prefer fruit pie, I must say that David has a chocolate brownie-cake-spectacularness in his fruit desserts book that rocks my world.
Alas, that book suffered damage in storage and had to be thrown away.
When I say I would to win the new book, and that David is one of my pastry heroes, I mean it on the deepest level.
Thanks for having such a great giveaway, because I know it taks great strength to part with that book!
My favorite dessert, when it's done well, is Bananas Foster. I don't know if David has that in his new book, but I heard that he is republishing his Chocolate-Dipped Coconut Macaroons, and that is fabulous! Hope you enjoyed the conference.
While I don't live in the US, I'd like to win this book for my delicious friend Alice who does. She and I have cooked together since we were old enough to pour and stir, and my favorite dessert is something that we often cook together – pineapple upside down cake. The recipe is from an oh-so-kitschy 70's cookbook, and yes we finish the pineapple rings with maraschino cherries – just like in the photo.
We'll be spending her birthday together in Maine and this book would be the perfect gift for our week together!
Oh I'd love this book! Favorite desserts? I love davidson creative ice cream recipes! In general, I love cream pies, anything chocolate, and really good croissants. Thanks for the exciting giveaway, Dianne!
I agree with Nic from Wheeling Gourmet here. I understand that most of your readers come from North America, but the days of uncharted maps and "there be dragons" are long gone. Sure postage is a hassle, I understand why you're excluding your Australian, NZ, British, European, Asian, South American etc readers, but to single out " fellow Canadians" as worthy of apology only makes your more distant readers feel more excluded. Especially if you're going to talk about Anzac cookies which after all originated with the Aussies and New Zealanders. (ANZAC = Australian and New Zealand Army Corp). I think giveaways are great, I don't mind that I can't be involved, but it does make me feel sad that you don't even consider we exist!
French macarons….love them. Also really like David's recipe for caramelized white chocolate. mmm…skeptical until I tried it.
Banana Cake with Mocha Frosting and Salted Candied Peanuts on page 62. Yum!
I purchased this cookbook for myself and 5 friends, it is wonderful!!
i live in europe, but would love love love to win this cookbook for someone i know in the states.
my fav dessert? ice-cream. i'm thinking of testing every single recipe form david's book. halfway through!
My favorite dessert is Tiramisu. *drools*
I will always eat anything with lemon in it so the Lemon Creme Tart at Tartine in San Francisco was probably one of the best things I have ever eaten. It took us all day running around the city to get there, but it was so worth it in the end. Truly amazing.
And if all else fails, there is always my (almost) famous chocolate-white chocolate chip cookies. Yummy…
Fruit with freshly whipped cream for splurge days.
Cheesecake for the days when I'm being really, really bad.
Picture this: There I am, a small boy in the north of England, about as bleak a food wilderness as you can imagine in the 1970s. (Now I live in NYC, by the way.)
For pudding we would get things like Angel Delight, which was a dry mix blended with milk and refrigerated to create a mousse. Then I loved it. Now, I shudder. One day my grandparents took my brother and I to York, the nearest city of any size and we went to a hotel for lunch. When the waiter asked if we wanted dessert, and we said we did, he replied with words that would transform my thinking about puddings forever. He said, "I'll bring the trolley." And he wheeled over a trolley filled with amazing desserts (say what you want about British food, but we're champions when it comes to the sweet stuff). I had a meringue in the shape of a swan, filled with strawberries and cream. As a result, to this day pavlova remains, for me, the ultimate dessert.
Fresh ginger cake!!!!! I bake gluten free and it is still my favorite!!
I've served it sometimes with whipped cream, with a handful of chopped
crystallized ginger thrown in- the ginger softens to little jellies.. fab.
Perfectly ripe mixed berries covered with soft clouds of zabaglione. My mouth waters just writing it.
My favorite dessert is green tea ice cream. I grew up in San Francisco and was often the lone Jewish kid in a mostly Asian class. Food gave me a platform of mutuality. Kids would flock to the local ice cream parlor after school and get red bean, melon or green tea. If I had the money I was right there with the group. Red bean was a disaster (something about the texture of re-ried beans being sweet and frozen remains unappealing) but I loved green tea. At home we use to eat neapolitan ice cream – which had that vaguely chemically aftertaste. Green tea ice cream was exotic and refreshing. As an adult I've transferred this adoration to mochi with green tea ice cream in the middle. Mmm.
Fresh Ice cream
Favorite dessert? Mocha Creme Brulee at my favorite local restaurant, Bistro Europa (in Buffalo).
Favorite dessert would have to be pavlova with balsamic strawberries. I could have it daily! Ice cream is another obsession. Would love to have David's new book if for no other reason than to finally tear myself away from The Scoop for a while. That one is definitely on my top 10 list. Love his stuff!
Fantastic blog and cookbook. Would love to have entered but I am from 'down under'. Good luck with it David.
My favorite dessert treat in haiku:
Tasty caramel.
Challenging for dental work.
Reward for my tongue.
my favorite dessert is actually brownies from a box on a rare occation. i know this is sacrilegious but like all my other favorites foods it is based almost purely on sentimental reasons. it reminds me of the excitement of being a kid waiting for them to come out of the oven and how fancy i thought they were at the time makes me laugh.
Butterscotch budino at Pizzeria Mozza in Los Angeles
My favorite dessert is flourless chocolate cake with vanilla whipped cream
There is something so delightful and refreshing about a huge fruit salad full of all the fruits of summer and fresh herbs from my garden!
That said, I love ooey gooey cookies and anything chocolate!
My favorite dessert is an ice cream sandwich. Ice cream and cookies together, perfect.
LOL it depends on the season:
Summer- my mom's fabulous unbaked Strawberry cheesecake
In late summer/fall..my wonderful apple pie;
Julia Childs' "Aunt Helen's Pumpkin Pie" at Thanksgiving
In winter , Mary Lou Williams' famous Pecan Pie
and for a year-round chocoholic fix, my mom's famous Brazo de gitano…a flourless dark chocolate cake/mousse log….mmmmm
I love Davids recipes and would delight in winning his cookbook !!
I absolutely love perfectly ripe fresh fruit for dessert, especially if it includes mango and pineapple.
Vanilla frozen yogurt with Pim T's Vann cherries and blood oranges
I love David Lebovitz, his writing, his wit, his recipes. My favorite dessert is brownies, and I've been making various recipes for a dozen years trying to find THE recipe. It's a fun and tasty quest!
Dessert I dream about lovingly, is the dacquoise from Tartine in NYC. The best I make is a sunshine cheesecake, that has paperthin slices of candied orange peel fanned out like a flower atop a decadent creamy base. Although I love all complex desserts, one of the best from David is a simple twist on the classic rice krispie treat – done with white chocolate and peanuts!
My favorite dessert, hands down, are Sicilian profiteroles … filled with either chocolate or coffee gelato….drizzled with deep dark chocolate. There is nothing better for me.
Pineapple Upside Down Cake is my all time favorite dessert. I adore pineapples.
Oh, how I love David. And his recipes. And keep wondering how someone who dishes out such mouth watering desserts can remain so slim and in-shape. He must also advice on this aspect.
I bought his Perfect Scoop for christmas (gifted self) and so far has made 70 percent of the recipes. Naturally, every week my favorite dessert changes. Once it was the vanilla ice cream, then the fresh ginger ice cream (for which even David left a comment on my post), and then many passed, but currently it is the roasted banana ice cream with a hot chcocolate fudge spread over. Yumm.
So many lovely desserts to choose from, but the dessert I love best is old fashioned Southern Banana Pudding. Warm or cold, it is the ultimate in a dessert and comfort food. This is the dessert I turn to when I want something sweet or just want comfort. Whenever I am ill, I will struggle to my kitchen, whip up a batch and eat nothing but banana pudding until I am all better. Lovely vanilla custard alternating with sliced bananas and 'nilla wafers and covered in meringue. Mmmmm. I think I hear the siren call now…
I love ice cream!
Chocolate cake in any form or fashion!
We served a favorite dessert at our wedding – hazelnut dome cakes.
Nearly impossible to narrow it down to just one favorite, but I'll single out my recent obsession: sfogliatelle. During a recent vacation in Italy, I squealed with delight to find it, better tasting and at a fraction of the cost at the fancy boutique bakery back home. The combination of flaky pastry, creamy filling, and orange notes is heavenly! I sought one out each day of our trip, but found it harder as we went from south to north.
How to choose my favorite dessert? Today it's probably flourless chocolate cake with drifts of whipped cream, but tomorrow it could be berries. Or coconut cream pie. Or ice cream… So many sweets, so little time!
My favorite dessert has to be any kind of cheesecake. Creamy, with a fruity topping … yummmm
Ice cream. French vanilla ice cream. Simple but wonderful.
an orange, semolina, yogurt and olive oil cake.
Zest of oranges,ground almonds(or hazelnuts),semolina and a really fruity extra virgin olive oil make this Italian style cake my go to cake. Unassuming,simple but a slice of sunshine in my mouth.
One of my NEW favorite desserts is David's Dulce de Leche Brownies, served like a cake square with a really good vanilla bean ice cream scoop. DE-licious!
David's Chocolate Idiot Cake… a flourless chocolate cake… fudgy chocolate through and through… need I say more?
It is VERY hard to narrow it down. But my favorite dessert? Something in the flourless chocolate cake category…with vanilla ice cream and caramel sauce.
I am a big fan of grasshopper brownies.
One favorite dessert? I think I go through seasonal obsessions. Right now I can't wait for summer and homemade ice cream and any fresh fruit-filled pie, cobbler, etc.
My favorite dessert is a seasonal fruit tart. My six year old daughter picks the fruit in season and we mix equal parts almond paste and butter together and spread it on a sqaure of puff pastry. Top with the fruit, a sprinkling of cinnamon and sugar and bake! Simple and delicious!
Hmm, what a terribly difficult question! Marzipan maybe? Or cheesecake?!
Never met a dessert I didn't like, but if I had to choose, my heart (and tastebuds) will always favor a rustic fruit tart/galette.
My favorite dessert is an English Trifle full of fresh summer berries, lemony custard and rich whipped cream!
thanks for this offer! he is phenomenal and i can't wait to get my hands on this book. my favorite dessert shall always remain a simple white cake with white icing. so perfect.
hmm chocolate, my favorite vitamin!
Ahhhhhhh – I go through food obsessions. For a while I could not resist key lime pie (problematic since I live in Miami so it's everywhere), but currently my obsession lies with panna cotta, for which David Lebovitz has a great recipe!
Most of my favorite desserts are store-bought, because they're what I grew up loving. Kozy Shack Rice Pudding is at the top of the list (still, even though I've been working my way through every rice pudding recipe I can find to try to beat it!). I swear, I could eat gallons of that stuff and not be either full or sick of it. Closely following that, however, is my aunt's recipe for absolutely decadent brownies: a pound of chocolate, 8 eggs, 4c flour, 2c sugar, etc… you get the idea. Decadent. And even more so with coarse kosher salt on top!
Not to be cliche, but anything chocolate! Cake, cookies, brownies, ice cream…yum!
My favorite dessert is a nice warm chocolate chip cookie w/ walnuts. Yum.
I'm not sure that I could pick just one, but a really rich flourless chocolate cake, with some fresh, home made whipped cream, is definitely in my top 3.
Thank you for the great offer – Good Luck to everyone who enters!
My favorite all-time dessert is apple pie. I love it with cheese or raisins or just apples. It is not complicated to make and can be whipped up quickly for unexpected guests. You can get as creative with it as you want but really prefer the simplicity of the apple pie itself.
Hi David, I really want your new cookbook because I lent your book "The great book of chocolate" to a friend 4yrs. ago and he will not give it back. We love you here in Madison, Wisconsin!, your desserts get me through the cold winters. Linda Muller
Key lime pie!
my fav dessert is homemade fruit pie – preferably with some kind of berries, but as long as it's made with fresh fruit and not too much sugar i'm a happy camper. and no store bought pie, even from the best bakery, can compare to one made with love at home.
My preferences are seasonal, but one of my all time favorites is a gooey, caramelized Tarte Tatin with a dollop of whipped cream fortified with a splash of Calvados.
The chocolate pistachio millefeuilles at Restaurant du Palais Royal by the Louvre. It is amazing!!!
Plum ice cream! Oh my goodness it is so good. It always turns out Barbie Pink, but you can eat it in the dark if you can't get over it. 😉
My favorite is my mom's recipe for rhubarb cream pie. It's thick and custardy but not too sweet. She liked to use a mix of green and red stalked rhubarb and I now grow descendents of her rhubarb patch in my own backyard. Made with her foolproof pie crust recipe passed down from her mother, it's unspeakably good.
daquoise! with hazelnuts, almonds, and chocolate…
The ones that will be sorry will not only the the fellow Canadians, but the rest of the world too….(Yes, there IS a world out there….) I understand the question of shipping costs but this is like having someone organising a party to which you'll never be invited….just because you don't live nearby….I think giveaways and contests SHOULD include all the world, after all the world reads your sites, not only Americans….For example, I bought your book, that is not available in France, I took the trouble of ordering it in an English bookstore (by the way the same bookstore where D Lebovitz will sign his books…) but now I do not exist for a giveaway…!!! bizarre, isn't it? By the way, Mr Lebovitz lives in Paris now, did he stop existing because he doesn't step the American ground???
Obviously I AM upset at this instance of provincialism. In a globalised world, where shipping a book costs "almost nothing" , not sending a book, not even to "your fellow Canadians" seems like too much….
Save your shipping costs…as far as I am concerned, I can still buy that book with my pocket money !!!
favorite dessert = coffee ice cream. homemade, of course.
Thank you for the offer. My favorite dessert……anything that is made with phyllo!!!
A rich, flourless chocolate cake with unsweetened, espresso whipped cream. Yummmmmmm……
first – i do adore david's site and have his book on my amazon list. i'd certainly love a free copy :).
my favorite dessert? that's so hard…. but i am a sucker for bread pudding or any sort, or ice cream. maybe both???!!
Favorite dessert…would have to be big, gooey chocolate chip cookies or chocolate cake! My mouth is watering!
I'm torn…but my favorite dessert is probably cheesecake in any of its incarnations. But lately, I find myself craving Cappuccino Fudge Cheesecake.
Or, ya know…ICE CREAM is always good!
I'm from Johnny Appleseed country, so my favorite dessert is warm, homemade apple pie – with a scoop of vanilla ice cream, of course!
Chocolate mousse!
Chartreuse soufflé with pistachio creme anglaise at L'Atelier in Las Vegas!
I've worked my way through a large number of David's ice cream recipes in The Perfect Scoop and have loved every one of them! So I guess my answer is ice cream
One of my fav dessert would be from D. lebovitz's chocolate brownie base with cream cheese swirl topping… yummm! His recipes are some that I know I can depend upon!
Thanks for the giveaway can't wait to see who wins!
happy baking!
The Tia Maria Torte from Baked Expectations in Winnipeg, Manitoba, Canada– amazing!!
Yellow cake with choclate frosting!
Favorite dessert – German Chocolate Cake by David Lebovitz!
I would have to say it's a toss up between nocciola (hazelnut) gelato and tiramisu. But, because I can make tiramisu quickly at home, nocciiola is much more of a treat!
Lemon-blueberry panna cotta, as served at Gautreau's in New Orleans.
I am moody, so it changes frequently, but the two that I return to over and over are Nutella on shortbread cookies or mascarpone cheese and lingonberry preserves spread on water crackers – both are simple, easily adjust to taste and are do-it-yourself at the table.
My favorite dessert is the cream puffs my mom makes. Thanks for entering me.
My first choice is white chocolate cake with strawberries. A close second is granny smith apple pie.
It must be some sort of ice cream. I'd go for pistachio, but no, rather this: good-quality vanilla ice cream with sliced bananas and Belgian chocolate sauce.
That, or a huge chocolate chip cookie.
My favorite desert is definitely sour cherry crisp with a scoop of vanilla ice cream. Very hard combination to beat in my mind.
OK, I'm going to enter anyway and if I win you can ship it to my parents-in-law… I would love to get a free copy of David's new book!
Banana cake with chocolate- brandy frosting, or a berry galette out of the Baking with Julia cookbook when the berries are most sublime and the summer sun renders one lazy and stupid.
My favorite dessert is strawberry shortcake. I just love the combination of strawberries, angel food cake, and cream. Thank you so much for hosting the giveaway!
Favorite dessert is the ginger cake from the first edition of "Room For Dessert," which I used to own before my daughter took it with her when she moved to NY(!*#@!). The ginger cake recipe is now scribbled on a scrap of paper that's been passed around from friend to friend to friend making it look like it crossed the Rockies in a covered wagon in the 1800's. The ginger cake is a hit with everyone. I'd love a copy of "Room For Dessert." I know there must be some other spectacular recipes in it.
Oops forgot to mention my fave dessert – a big, chewy, gooey chocolate chip cookie.
My favorite dessert is homemade ice cream. My favorite gadget is my ice cream maker, I now could not live without it.
I can not count the number of times I have made eggless mint chocolate chip ice cream over the last 2 years.
Mom's pound cake. Simple, reliable, and what I crave when I crave dessert.
chocolate. and ice cream. period.
buttery brioche with cinnamon and raisins
I will have to say, hot apple pie with a dollop of ice cream.
hard question…i'm going with apple pie. double crust flaky pastry with fresh apples and cinnamon= a whole lotta yumm!
I absolutely loved the Perfect Scoop. The chocolate ice cream was to die for, and I was able to adapt the vanilla to make my favorite ice cream flavor in the whole world…Hokey Pokey. Yummm….
My favorite desserts are seasonal fruit/berry crisps with milk or vanilla ice cream.
I'm a big fan of cobblers and crisps!
Chocolate chip cookies or chocolate peanut butter ice cream…ps David is a god (not God but more like a minor luminous figure.)
It's kind of like two questions. My favorite changes with the wind, or the moon, or something astrological depending on too many factors, but if I were asked if it was to be my last day on earth and I could have just one dessert, what would it be? My answer is clear; a warm, dense, homemade brownie with mint chocolate chunk ice cream, topped with hot fudge, the kind that sort of coagulates a bit when it hits the ice cream, whipped cream and nuts. Hold the yuckky maraschino cherry please.
hi – dark chocolate cake. yum.
I'm not much on sweet things. I really don't care for the taste of any form of sugar, so desserts are not that high on my list. However, I do have a wonderful memory of the one of the best desserts I've ever eaten…
Tony's Restaurant in St. Louis was the place to eat about 10 years ago. My then husband and I dressed up in our finery and headed to Tony's. It was our anniversary, so we splurged with the Romantic Dinner for Two, complete with wines by the course, and dessert.
I was also a vegetarian, but managed quite nicely to work around the prix fixe menu which included a beef entree. Our waiter, being aware of my herbivore status, was a little distraught at the accomodation I was making. When he discovered I didn't eat sugar, however, he was really concerned and went straight to the top: Tony himself appeared table side!
He graciously introduced himself, asking after our meals, commenting on our attire. Looking to my husband, he said, "And have we decided on a dessert choice?" My husband looked to me, and Tony continued: "I understand you do not eat sugar. I respect that, and yet, I feel it would be sad for you to miss our dessert experience. Would you allow me to create something just for you?" His eyes twinkled in the low lights. I lowered my own and said a soft, "Why, thank you!", secretly pleased to the core.
A short time later, Tony came sailing to our table, with a white cloth draped over his arm, bearing a silver dessert cup on a small plate. I smiled with delight when he set it in front of me, for it contained a generous serving of unsweetened creme fraiche – just whipped up for me, I was assured – with a touch of vanilla bean extract. Crowning this beautiful achievement was an assortment of piquant wild blueberries and sweet yet slighty tart strawberries, with a gorgeous cluster of tiny, flavorful champagne grapes lying enticingly to the side. Absolute nirvana – and perfect!
As I sighed and yummed over each delicious, sensual bite, my husband, with his something-or-other creme brulee, kept glancing longing at the bowl. Finally I relinquished a taste to him. It was my anniversary gift in that moment! He closed his eyes, savoring the blend of melting creme fraiche with the cool crispness of the berries and grapes. He then looked at me and said, "You're right. This is the perfect dessert!" To date, I haven't dared tried to recreate it. You cannot mess with perfection, even if it is but a memory.
My mother's cherry pie!
Sour cherry pie! So hard to get unless you have your own tree.
Definitely brownies — so good right out of the oven, a few days later (if they last!) or as the basis for a sundae!
Key Lime Pie! Not the kind with gelatin. Gross! Real key lime pie! No artificial colors and no thickeners.
As long as there's cream and eggs I know I will like it … frozen or cooked … homemade ice cream, creme brulee, pudding, custard, tapioca, pot au creme, panna cotta …
My great grandma's peach cobbler. Yum!
Chocolate flecked angelfood cake with raspberries!
Hmm.. favorite dessert….. make that favorite dessert(s)!!!
My Grandmother's Florence Cake – thin, flaky pastry with a thin layer of raspberry preserves, sponge cake and a buttercream frosting!!
David Lebovitz's Lemon Tart – Easy, beautiful and, needless to say, absolutely delicious !!
ohhh…too many to choose: paris brest, tarte tatin, pear-almond tart, buttery lemon pound cake, flourless chocolate cake, italian almond cake, really dense brownies, chocolate marble cheese pie, philadelphia butter cake, vanilla bean anything….and beautiful, fresh, crusty bread! these are just a few of the favorites that i make for us but especially whenever we have someone staying with us – a good excuse to splurge on calories!
Almost any tweaked classic, although ice cream is pretty big with me.
My favorite dessert hasn't changed since I was a kid: a warm chocolate chip cookie with a glass of milk.
hot fudge pudding cake with vanilla ice cream, warm and gooey and chocolatey and ice cream to cleanse the palette for the next bite. YUM!
Warm rhubarb pie (with a little heavy cream drizzled on it). For that matter, anything with rhubarb. I find it charming that it's a fr-egetable that reminds me of the pies my grandmother in the Midwest used to make (it was a favorite of hers too) and also is somehow so beloved by the French. (I was so thrilled to go to Paris for the first time to find… rhubarb yogurt.) The bite of the rhubarb, bolstered by a good buttery pie crust, and tempered and enriched by the cream…Oh, just the thought of rhubarb sends me into a state of near ecstasy.
My favorite dessert is ice cream, particularly any salted caramel ice cream, but any ice cream flavor works for me!
Warm rhubarb pie with a little heavy cream drizzled on it. (For that matter, anything with rhubarb.) I find it charming that it's a fr-egetable that reminds me of the pies my grandmother in the Midwest used to make (it was a favorite of hers too) and also is somehow so beloved by the French. (I was so thrilled to go to Paris for the first time to find… rhubarb yogurt.) The bite of the rhubarb, bolstered by a good buttery pie crust, and tempered and enriched by the cream…Oh, just the thought of rhubarb sends me into a state of near ecstasy.
Well, I'm a Southern girl, so this won't surprise you: PECAN PIE!
Moist chocolate gâteau with raspberry buttercream frosting between the layers and just enough dark chocolate ganache poured on top to barely drip down the sides. Très magnifique!
Ice cream! Maybe even David's Chocolate Coconut Sorbet from The Perfect Scoop.
peach custard pie.
I love the blueberry and white chocolate tart. So good…
At the moment… carrot cake with maple cream cheese frosting.
It is not fer, but you have not write anything about living in Macedonia, so my favorite desert is chocolate cake.
I will understand if you not choose me.
Any way thank you for asking.
loooove dessert so it's so hard to pick a favorite…but I'll never turn away from a chocolate chip cookie.
All notions of health and idealism aside, nothing beats a seven-layer, decadent, slightly fudgy-but-not-too-dense chocolate cake still cold from the refrigerator or only slightly thawed from the freezer – you know, the kind of cake you approach thinking there's no way you could possible eat the entire slice of, and then you somehow -remarkably and heroically – find a way.
My grandmothers Three Layer Chocolate Cake with Seven Minute Frosting. She made this cake for my fathers birthdays and for the past 20 years I've been making this cake for him on his birthday and other occasions.
My husband's tiramisu recipe is the best. Nice and light and creamy. A great way to finish off a lovely romantic dinner.
I love chocolate chip cookies!
When I was 17 I went to a French restaurant that had fried strawberries in vanilla sauce. I've never seen then since sadly. This the dessert I have dreams about and have never once come close to finding a recipe that works.
Creme Brule comes to mind but more then that the homemade Indian desserts my mother & grandmother made.
Chocolate cake with raspberry filling covered in chocolate ganache. Mmmmm…
Homemade cheesecake that has some sort of fine dark chocolate component . . . either in the crust, the filling, or the topping. Then again . . . maybe a really good, fresh fruit shortcake with whipped cream . . .
My answer to the "favorite dessert" question will vary depending on the day. Lately I've been craving zabaglione with fresh berries… guess I'm eager for summer's bounty to begin. Thanks for the giveaway!
Being from Mauritius, it is a napolitaine. Great with a nice cup of English tea. And you can't find it anywhere else.
My favorite one-time dessert is the apple pie that I had from the little kiosk at the ferry back from the Isle of Skye. I was cold and wet and the pie was warm and perfect with slightly melty ice cream (I don't know how – it was THAT cold at the time). Eaten out of a styrofoam container with a spork, nothing has ever come close to topping it.
My favorite dessert in general is anything, and I mean anything!, with dulce de leche. Seriously, anything.
at the moment, carrot cake with maple cream cheese frosting!
peach cobbler with vanilla bean ice cream
Hands down, it's carrot cake.
Shortbread! It melts…
I adore David Leibovitz! My favorite dessert is chocolate ice cream with lots of nuts!
That would be profiteroles!
It's very hard to lay my allegiance with one favorite dessert I love trying new recipes, so I'm usually pretty pleased with the latest result (my husband will agree with me!). If I must choose, I have to say German chocolate cake is pretty amazing!
Lately it's been caramel apple cheesecake with a scatterig of pecans, but that could change now that strawberry season has arrived!
I had the most fun making red velvet cupcakes with my granddaughter Sadie in San Francisco from the cookbook "Magnolias" (NYC bakery). Flour flying, red Wilton in her hair and it took half a day with the cooked frosting and all…but these were to die for!!!
Mmmm, that's easy! Dark chocolate truffles.
Oh, I have been dying to get my hands on this book! My favorite dessert is probably ice cream, Ben and Jerry's Chunky Monkey being my favorite in that category. But really, if you put something chocolate in front of me I'll probably decide THAT's my favorite desert… that day.
I love fresh fruit desserts. Right now I'm longing for peach galette.
For my ridiculous sweet tooth, I'd have to go with pecan pie.
Home-made vanilla ice cream.
David Lbovitz's books are wonderful! I'd love to have this one. As for my favorite dessert: warm homemade chocolate chip cookies with vanilla ice cream.
Wow, don't know if I have just one favorite dessert! I have such a raging sweet-tooth that for me its more about controlling those urges (especially now that I'm middle-aged and needing to lose some weight). Lately, the desserts that call most to me are ones with caramel, especially salted caramel. I've moved on from chocolate to more nutty, caramely, buttery cravings, especially anything that goes with a nice cup of tea.
My favorite, everything chocolate!
Revani with icecream
Oven baked rice pudding
David's famous Fresh Ginger Cake…
but the Apple-Pear Crisp sounds delectable.
This is an impossible contest!
Warm persimmon pudding with raisins topped by a dollop of whipped cream.
Anything with lemon flavor….love, love, love it.
My favorite is definitely a cookie pie recipe I found and changed a bit to my liking. It has a chocolate chip cookie crust, semisweet/milk chocolate ganache filling, with a heap of fresh whipped cream on top. Absolutely delicious. But my new favorite may be David's chocolate chip pecan cookies.
Can I pick a couple faves? I love pear and almond tart, but sometimes tarte Tatin is the only thing that hits the spot.
Of course, I'd never turn down good chocolate, but it's hard to find where I live.
I love cupcakes – any kind…any where!
Choosing a favorite dessert is nearly as excruciating as trying to decipher an Ozzy Osbourne interview. However, if it means getting a shot at winning David Levovitz's book, then I'm game. Give me a bowl of silky chocolate mousse any day of the week and I just might be transported to a plane where Ozzy and I understand each other.
Ok, if I have to make a "Sophie's Choice," I'll go with homemade rugelach. I have perfected the family recipe that has sour cream, NOT cream cheese in the dough, tart apricot jam, walnuts, golden raisins and lots of sugar and cinnamon both inside and on top after they're rolled. The best part is getting to eat the "drippings" right out of the oven before all the leftover jam, nuts and raisins stick to the parchment paper and will no longer come off. On the other hand, I made David Lebovitz's cheesecake brownies once, and they were to die for. No! I'm sticking with the rugelach!! Final answer? Rugelach!!
Choosing a favorite dessert is like choosing a favorite child – somedays it's easier than others. Today is a tough day but I'm going to go with pound cake with any number of accompaniments. Today was strawberries and brie. But i love it with chocolate, with ice cream, whipped cream or just plain by itself no strings attached!
Pecan pie – is my favorite.
Ohhh, so hard to choose one. But it has to be with whipped cream!
anything that has chocolate in it. i really really love this chocolate mousse from Almondine bakery in Brooklyn. absolutely UH-mazing. i was going to cry because it was so good. so for now, that is my fave dessert.
No chance of winning this book as I live outside the US, but I am going to post anyway!
French Lemon Tart
Oh, so many good desserts and i have to choose one? Then i think i will go with Rhubarb and starwverry Pie.
Ice cream, chocolate pot de creme from the Slated Door, shaker lemon pie, anything with marzipan or almond…homemade pumpkin pie with piles of whip cream.
Awesome giveaway! Crossing my fingers and toes…
Lemon squares are my very favorite with David's cheesecake brownies and raspberry clafouti from The Sweet Life in Paris close behind…
Virtually an impossible question for me to answer, but most of the time it's something chocolate, a chocolate marquise with a chocolate sable to add a little crunch to the dense and creamy marquise.
I love cheesecake!
French Silk Pie…but as others have stated, choosing just one favorite…not easy ;D
Sorry, the comment form is closed at this time.


text:
Video: Douglas Black charged in theft of campaign signsWritten by Zach Davis | | firstname.lastname@example.com
The Lucas County Democratic Party released video by two volunteer campaign workers on Aug. 24 showing a man destroy Michelle Wagner's judicial campaign signs. Since June, 55 signs have been stolen or destroyed, including four in Wagner's own backyard.
Toledo native Douglas Black has been charged by the Toledo Municipal Court with stealing and destroying signs. The total damage from losing 55 signs has cost the campaign $1,484 of damage. The majority of the signs have disappeared or been destroyed since June in the area of Reynolds Road and Heatherdowns.
To prevent future thefts in her backyard, Wagner had a wooden structure built to hold the signs in her trees.
"If it would have stopped 2-3 weeks ago it probably would have just been dropped and forgotten about but any time we put up a sign it was gone the next day," said Wagner for Judge Campaign Manager Josh Thurston. "Whoever was doing this was going into people's yards and destroying their property. Not only that they are hindering free speech."
Black, 66, has been charged with two separate charges including "petty theft with purpose to deprive knowingly obtain or exert control without consent of owner" as well as "criminal damaging or endangering knowingly cause or create substantial risk of physical harm."
Thurston said the Wagner campaign had no previous knowledge of the alleged man but says "it's definitely political and definitely targeted." He claims that in the video, the man passes a campaign sign for Wagner's opponent, Mark Davis, before targeting another of their signs.
"What is it about this guy that elicits this kind of response, a hard-working mother of three that works 40-50 hours a week?" Thurston asked.
Thurston also denied the allegations raised by Davis and Lucas County Republican Chairman Jon Stainbrook that Wagner's campaign had accused them of being involved.
"There have been no public accusations," Thurston said. "We have people out there that are supportive of Michelle and we can't control what they say on things like Facebook. I am the only paid campaign worker and Michelle is the candidate and neither of us has publicly said that Mark Davis is doing this, Dave Toska is doing this or Jon Stainbrook is doing this.
"All we are saying is it really looks fishy to see where these are happening, how they are happening and when they are happening."
Thurston was involved in Marcy Kaptur's campaign last year for U.S. Congress where opponent Rich Iott was photographed wearing a Nazi uniform in reenactment. A storm of controversy ensued but Thurston said that even that incident didn't compare to the sign controversy.
"The Rich Iott stuff was amazing, it was a crazy thing to be a part of but this is criminal," Thurston said. "Rich Iott made a bad judgement by reenacting Nazis but this is beyond that this is criminal. If this was a coordinated thing it is a big deal. I'm not saying it is or not, but it doesn't pass the smell test."
Black has been summoned to appear for arraignment on Sept. 2 at 1 p.m. at the Toledo Municipal Court.
"We are going to let the courts do what they do best," Thurston said. "He will have his day in court and I look forward to that."

subset: commoncrawl


text:
October is a busy time of the year. And although the calendar says fall, it is a month when we begin planning for the next season.
That's particularly true for the Johnson City Press Christmas Box. Well before the first handful of candy is distributed on Halloween night, work will have already begun for the 31st annual Christmas Box drive.
Actually, the sign-up for the Christmas Box and Angel Tree began this week for low-income residents in Washington and Unicoi counties. Carter County families and seniors can register at Oak Street Baptist Church, 804 Oak St., Elizabethton. from 4 p.m. to 6 p.m. on Monday; from 9 a.m. to noon Tuesday; and from 9 a.m. to noon on Nov. 1.
As Press staff writer Sue Legg Guinn reported last week, the cost of this year's Christmas Box distribution is estimated to be $53,000. Letters to past contributors of the project were mailed out this week, and we are asking those who have supported the Christmas Box in the past to continue their generosity.
We are also asking for help from those of you who have never given to the Christmas Box. This is a good year to begin a new tradition of giving.
Donations may be mailed to the Johnson City Press Christmas Box of Northeast Tennessee, P.O. Box 1387, Johnson City, TN 37605.
We have a daunting task before us this year, one that we must undertake without the help of our good friend and Press coworker, John Castle, who died in March. For many years Castle was not only the face, but the heart and soul of the Christmas Box program. He did what was needed to be done. If it was filling a food box — he filled it. If it was delivering a box — he delivered it.
With John's help, and with the help of so many others like him, we were able to serve a record 1,900 area families last Christmas. The need this year is just as great, and we can use all the volunteers we can get to help assemble and distribute food boxes.
For more information about the Christmas Box and how to help, call Ron Tipton at the Press at 929-3111, ext. 302.


text:
Edward Norton Replaced As Hulk In AvengersMovie
Marvel Studios release statement about dropping actor, Norton responds
Edward Norton is out as the green, gentle giant.
Kevin Feige, Marvel Studios' president of production, sent a message directly to HitFix.com that confirmed the cast change for the part of Bruce Banner in "The Avengers," leaving Norton out of the Marvel character team movie.
Our decision is definitely not one based on monetary factors, but instead rooted in the need for an actor who embodies the creativity and collaborative spirit of our other talented cast members," Feige said. "'The Avengers' demands players who thrive working as part of an ensemble. We are looking to announce a name actor who fulfills these requirements, and is passionate about the iconic role in the coming weeks.
The move to change actors for an existing role is reminiscent of the decision to replace Terrance Howard with Don Cheadle in the role of Lt. Col. James Rhodey Rhodes in the Iron Man franchise. Feiges statement suggests that because the "Avengers" film is an ensemble piece, there were concerns with Nortons ability to work well with others.
Norton took to his Facebook page to release a response to the outpouring of fan support he received. In it, he thanked his fans and thanked the studio for the opportunity to be a part of the franchise. Although he expressed hope to be a part of the ensemble, he wished the next person to portray Banner well.
At the end of his note, Norton took on a playful tone. I grew up with Banner and Hulk and have been a fan of every incarnation. Im really proud, and very blessed, to have been one of them and will be thrilled to see him live on through other actors. Hulk is bigger than all of us, thats why we love him, right?
The studio will conduct its search for the new Bruce Banner to take part in "The Avengers," which will be directed by Joss Whedon. As of now, the cast still includes Robert Downey Jr. as Iron Man, Chris Evans as Captain America, Samuel L. Jackson as Nick Fury, Scarlett Johansson as Black Widow and Chris Hemsworth as Thor.
About the Author


text:
MAB4401 | Anti-Oct-4 Antibody, clone 10H11.2
Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2 validated for use in WB, FC, IC, ELISA.
Detect Oct-4 using this Anti-Oct-4 Antibody, clone 10H11.2 validated for use in WB, FC, IC, ELISA. Less<<
|Replacement Information
Ofertas especiales
Tabla espec. clave
|Species Reactivity
|Key Applications
|Antibody Type
|Monoclonal Antibody
|Product Information
|Purified Ascites presented as a liquid in PBS with 0.1% sodium azide.
|Physicochemical Information
|Materials Information
|Toxicological Information
|Safety Information according to GHS
|Safety Information
|Storage and Shipping Information
|Storage Conditions
|Stable for 1 year at from date of receipt.
|Packaging Information
|Material Size
|100 µg
|Transport Information
|Supplemental Information
Certificados de análisis
|Número de lote
|Anti-Oct-4, clone 10H11.2
|Anti-Oct-4, clone 10H11.2 - 2392284
|Anti-Oct-4, clone 10H11.2 - 1991253
|Anti-Oct-4, clone 10H11.2 - 2066310
|Anti-Oct-4, clone 10H11.2 - LV1679970
|Anti-Oct-4, clone 10H11.2 - LV1786447
|Anti-Oct-4, clone 10H11.2 - NG1865606
|Anti-Oct-4, clone 10H11.2 - NG1927045
|Anti-Oct-4, clone 10H11.2 - PSO1553824
|Anti-Oct-4, clone 10H11.2 -2512486
|Anti-Oct-4, clone 10H11.2 -2580657
|Anti-Oct-4, clone 10H11.2 -2610382
Referencias bibliográficas
|Visión general referencias
|Pub Med ID
|High-content imaging-based screening of microenvironment-induced changes to stem cells.
Sebasti Vega,Er Liu,Parth J Patel,Anthony B Kulesa,Aaron L Carlson,Yanrui Ma,Matthew L Becker,Prabhas V Moghe
Journal of biomolecular screening 17 2012
Mostrar resumen
Effective screening methodologies for cells are challenged by the divergent and heterogeneous nature of phenotypes inherent to stem cell cultures, particularly on engineered biomaterial surfaces. In this study, we showcase a high-content, confocal imaging-based methodology to parse single-cell phenotypes by quantifying organizational signatures of specific subcellular reporter proteins and applied this profiling approach to three human stem cell types (embryonic-human embryonic stem cell [hESC], induced pluripotent-induced pluripotent stem cell [iPSC], and mesenchymal-human mesenchymal stem cell [hMSC]). We demonstrate that this method could distinguish self-renewing subpopulations of hESCs and iPSCs from heterogeneous populations. This technique can also provide insights into how incremental changes in biomaterial properties, both physiochemical and mechanical, influence stem cell fates by parsing the organization of stem cell proteins. For example, hMSCs cultured on polymeric films with varying degrees of poly(ethylene glycol) to modulate osteogenic differentiation were parsed using high-content organization of the cytoskeletal protein F-actin. In addition, hMSCs cultured on a self-assembled monolayer platform featuring compositional gradients were screened and descriptors obtained to correlate substrate variations with adipogenic lineage commitment. Taken together, high-content imaging of structurally sensitive proteins can be used as a tool to identify stem cell phenotypes at the single-cell level across a diverse range of culture conditions and microenvironments.
|Dynamic profiles of Oct-4, Cdx-2 and acetylated H4K5 in in-vivo-derived rabbit embryos.
Chien-Hong Chen,Jie Xu,Wei-Fang Chang,Chia-Chia Liu,Hwa-Yun Su,Y Eugene Chen,Fuliang Du,Li-Ying Sung
Reproductive biomedicine online 25 2012
Mostrar resumen
This study documents the spatial and temporal distribution of Oct-4, Cdx-2 and acetylated H4K5 (H4K5ac) by immunocytochemistry staining using in-vivo-derived rabbit embryos at different stages: day-3 compact morulae, day-4 early blastocysts, day-4 expanded blastocysts, day-5 blastocysts, day-6 blastocysts and day-7 blastocysts. The Oct-4 signal was stronger in the inner cell mass (ICM)/epiblast cells than in the trophectoderm (TE) cells in all blastocyst stages except day-4 expanded blastocysts, where the signal was similarly weak in both the ICM and TE cells. The Cdx-2 signal was first detected in a small number of TE cells of day-4 early blastocysts, and became evident in the TE cells exclusively afterwards. A consistently strong H4K5ac signal was observed in the TE cells in all blastocyst stages examined. In particular, this signal was stronger in the TE than in the ICM cells in day-4 early blastocysts, day-4 expanded blastocysts and day-5 blastocysts. Double staining of H4K5ac with either Oct-4 or Cdx-2 on embryos at different blastocyst stages confirmed these findings. This work suggests that day 4 is a critical timing for lineage formation in rabbit embryos. A combination of Oct-4, Cdx-2 and H4K5ac can be used as biomarkers to identify different lineage cells in rabbit blastocysts. There are very limited studies on key transcription factors and epigenetic programming events in preimplantation-stage rabbit embryos. Previously we reported the spatial and temporal distribution of Oct-4 and acetylated H4K5 (H4K5ac) in-vitro-cultured rabbit embryos. In the present study, we report the similar distribution patterns of Oct-4 and H4K5ac using in-vivo-derived rabbit embryos at different stages: day-3 compact morulae, day-4 early blastocysts, day-4 expanded blastocysts, day-5 blastocysts, day-6 blastocysts and day-7 blastocysts. The Oct-4 signal was stronger in the inner cell mass (ICM)/epiblast cells than in the trophectoderm (TE) cells in all blastocyst stages except day-4 expanded blastocysts, where such signal was similarly weak in both the ICM and the TE cells. A consistent strong H4K5ac signal was observed in the TE cells in all stages examined. In particular, this signal was stronger in the TE than in the ICM cells in day-4 early blastocysts, day-4 expanded blastocysts and day-5 blastocysts. Importantly, we also report, as far as is known for the first time, the distribution pattern of Cdx-2 in rabbit embryos. The Cdx-2 signal was first detected in a small number of TE cells of day-4 early blastocysts. It became evident in the TE cells exclusively afterwards. The present study provides novel information on key transcription factors and epigenetic events during rabbit embryo development, and demonstrates that a combination of Oct-4, Cdx-2 and H4K5ac could be used as biomarkers to identify different lineage cells in rabbit blastocysts.
|Oct-4B isoform is differentially expressed in breast cancer cells: hypermethylation of regulatory elements of Oct-4A suggests an alternative promoter and transcriptional start site for Oct-4B transcription.
Wang, Yajuan, et al.
Biosci. Rep., 31: 109-15 (2011) 2011
Mostrar resumen
The human Oct-4 gene has three isoforms, Oct-4A, Oct-4B and Oct-4B1, which are thought to be derived from alternative splicing. It remains controversial whether the Oct-4 gene is expressed in cancer cells. Expression of Oct-4A is regulated by two elements, the PE (proximal enhancer) and DE (distal enhancer), but the expression and regulation of Oct-4B are not well known. Here, we firstly report that Oct-4B is expressed at low levels in MCF-7 cells, while the Oct-4A gene is inactivated. By analysing the function of different promoter constructs and the DNA methylation status of three regulatory regions, we demonstrate that the Oct-4A gene in MCF-7 cells is repressed by epigenetic control rather than transcriptional control. In addition, we speculate that the transcription of Oct-4B in MCF-7 cells is differentially regulated by additional regulatory elements. This work will enhance the understanding of Oct-4 gene in differential regulation.
|Characterization of differential properties of rabbit tendon stem cells and tenocytes.
Jianying Zhang,James H-C Wang
BMC musculoskeletal disorders 11 2010
Mostrar resumen
Tendons are traditionally thought to consist of tenocytes only, the resident cells of tendons; however, a recent study has demonstrated that human and mouse tendons also contain stem cells, referred to as tendon stem/progenitor cells (TSCs). However, the differential properties of TSCs and tenocytes remain largely undefined. This study aims to characterize the properties of these tendon cells derived from rabbits.Artículo Texto completo
|Efficient derivation of embryonic stem cells from nuclear transfer and parthenogenetic embryos derived from cryopreserved oocytes.
Sung LY, Chang CC, Amano T, Lin CJ, Amano M, Treaster SB, Xu J, Chang WF, Nagy ZP, Yang X, Tian XC
Cell Reprogram 12 203-11. 2010
Mostrar resumen
Deriving histocompatible embryonic stem (ES) cells by somatic cell nuclear transfer (SCNT) and parthenogenetic activation (PA) requires fresh oocytes, which prevents their applications in humans. Here, we evaluated the efficiency of deriving ES cells from mature metaphase II (MII) and immature metaphase I (MI) vitrified oocytes, by PA or SCNT, in a mouse model. We successfully generated ES cell lines from PA (MII and MI) and SCNT (MII and MI) blastocysts. These cell lines expressed genes and antigens characteristic of pluripotent ES cells and produced full-term pups upon tetraploid embryo complementation. This study established an animal model for efficient generation of patient-specific ES cell lines using cryopreserved oocytes. This is a major step forward in the application of therapeutic cloning and parthenogenetic technology in human regenerative medicine and will serve as an important alternative to the iPS cell technology in countries/regions where these technologies are permitted.
|Ago2 immunoprecipitation identifies predicted microRNAs in human embryonic stem cells and neural precursors.
LA Goff, J Davila, MR Swerdel, JC Moore, RI Cohen, H Wu, YE Sun, RP Hart
PloS one 4 e7192 2009
Mostrar resumen
BACKGROUND: MicroRNAs are required for maintenance of pluripotency as well as differentiation, but since more microRNAs have been computationally predicted in genome than have been found, there are likely to be undiscovered microRNAs expressed early in stem cell differentiation. METHODOLOGY/PRINCIPAL FINDINGS: SOLiD ultra-deep sequencing identified >10(7) unique small RNAs from human embryonic stem cells (hESC) and neural-restricted precursors that were fit to a model of microRNA biogenesis to computationally predict 818 new microRNA genes. These predicted genomic loci are associated with chromatin patterns of modified histones that are predictive of regulated gene expression. 146 of the predicted microRNAs were enriched in Ago2-containing complexes along with 609 known microRNAs, demonstrating association with a functional RISC complex. This Ago2 IP-selected subset was consistently expressed in four independent hESC lines and exhibited complex patterns of regulation over development similar to previously-known microRNAs, including pluripotency-specific expression in both hESC and iPS cells. More than 30% of the Ago2 IP-enriched predicted microRNAs are new members of existing families since they share seed sequences with known microRNAs. CONCLUSIONS/SIGNIFICANCE: Extending the classic definition of microRNAs, this large number of new microRNA genes, the majority of which are less conserved than their canonical counterparts, likely represent evolutionarily recent regulators of early differentiation. The enrichment in Ago2 containing complexes, the presence of chromatin marks indicative of regulated gene expression, and differential expression over development all support the identification of 146 new microRNAs active during early hESC differentiation.Artículo Texto completo
|The intracellular distribution of the ES cell totipotent markers OCT4 and Sox2 in adult stem cells differs dramatically according to commercial antibody used.
Patricia A Zuk, Patricia A Zuk
Journal of cellular biochemistry 106 867-77 2009
Mostrar resumen
To characterize ES cells, researchers have at their disposal a list of pluripotent markers, such as OCT4. In their quest to determine if adult stem cell populations, such as MSCs and ASCs, are pluripotent, several groups have begun to report the expression of these markers in these cells. Consistent with this, human ASCs (hASCs) are shown in this study to express a plethora of ES pluripotent markers at the gene and protein level, including OCT4, Sox2, and Nanog. When intracellular distribution is examined in hASCs, both OCT4 and Sox2 are expressed within the nuclei of hASCs, consistent with their expression patterns in ES cells. However, a significant amount of expression can be noted within the hASC cytoplasm and a complete absence of nuclear expression is observed for Nanog. Recent descriptions of OCT4 transcript variants may explain the cytoplasmic expression of OCT4 in hASCs and consistent with this, hASCs do express both the OCT4A and 4B transcript variants at the gene level. However, discrepancies arise when these three pluripotent markers are studied at the protein level. Specifically, distinct differences in intracellular expression patterns were noted for OCT4, Sox2, and Nanog from commercial antibody to commercial antibody. These antibody discrepancies persisted when hMSCs and rat ASCs and MSCs were examined. Therefore, confirming the expression of OCT4, Sox2, and Nanog in adult stem cells with today's commercial antibodies must be carefully considered before the designation of pluripotent can be granted.
|Targeting cancer stem cells through L1CAM suppresses glioma growth.
Bao, Shideng, et al.
Cancer Res., 68: 6043-8 (2008) 2008
Mostrar resumen
Malignant gliomas are lethal cancers that display striking cellular heterogeneity. A highly tumorigenic glioma tumor subpopulation, termed cancer stem cells or tumor-initiating cells, promotes therapeutic resistance and tumor angiogenesis. Therefore, targeting cancer stem cells may improve patient survival. We interrogated the role of a neuronal cell adhesion molecule, L1CAM, in glioma stem cells as L1CAM regulates brain development and is expressed in gliomas. L1CAM(+) and CD133(+) cells cosegregated in gliomas, and levels of L1CAM were higher in CD133(+) glioma cells than normal neural progenitors. Targeting L1CAM using lentiviral-mediated short hairpin RNA (shRNA) interference in CD133(+) glioma cells potently disrupted neurosphere formation, induced apoptosis, and inhibited growth specifically in glioma stem cells. We identified a novel mechanism for L1CAM regulation of cell survival as L1CAM knockdown decreased expression of the basic helix-loop-helix transcription factor Olig2 and up-regulated the p21(WAF1/CIP1) tumor suppressor in CD133(+) glioma cells. To determine if targeting L1CAM was sufficient to reduce glioma stem cell tumor growth in vivo, we targeted L1CAM in glioma cells before injection into immunocompromised mice or directly in established tumors. In each glioma xenograft model, shRNA targeting of L1CAM expression in vivo suppressed tumor growth and increased the survival of tumor-bearing animals. Together, these data show that L1CAM is required for maintaining the growth and survival of CD133(+) glioma cells both in vitro and in vivo, and L1CAM may represent a cancer stem cell-specific therapeutic target for improving the treatment of malignant gliomas and other brain tumors.
|Microporous membrane growth substrates for embryonic stem cell culture and differentiation.
Steven D Sheridan,Sonia Gil,Matthew Wilgo,Aldo Pitt
Methods in cell biology 86 2008
Mostrar resumen
As the field of embryonic stem cell culture and differentiation advances, many diverse culturing techniques will ultimately be necessary in order to fully reproduce the various environments these cells normally encounter during development. Although most of the work to date has been performed on solid plastic supports, this growth support has several limitations in its representation of the in vivo environment. Impermeable substrates force the cells to exchange their gas and nutrients exclusively through the top side of the cultured cells. In contrast, cells growing in vivo are exposed from several directions to factors from the blood, other cells, soluble factors, and liquid-air interfaces. Additionally, solid plastic presents a smooth two-dimensional surface that is not experienced in vivo. Therefore, the use of traditional plastic presents limitations upon normal cellular morphology, function, and differentiation. An important alternative to growth on solid plastic is the growth of cells on microporous membranes. One of the many advantages to cell growth on porous membrane substrates is their ability to provide a surface that better mimics a three-dimensional in vivo setting. A porous membrane allows multidirectional exposure to nutrients and waste products. In addition, the membrane separation of dual chambers allows for the coculture of cells of different origin to study how cells interact through indirect signaling or through providing a conditioned niche for the proper growth and differentiation of cell types.
|Human Stem Cell Systems
Licencias necesarias e Información técnica
|A Comparative Analysis of Human Embryonic Stem Cells Cultured in a Variety of Media Conditions
|Single transfection of a synthetic polycistronic self-replicative RNA yields high numbers of transgene-free human iPSCs
Ficha técnica
|Anti-Oct-4, clone 10H11.2 - Data Sheet
|STEMCCA Lentivirus Reprogramming Kits
|Efficient generation of transgene-free human and mouse iPS cells using a cell-permeant Tat-Cre protein


text:
Pronunciation: /ˈsenəˌɡôl/
Definition of Senegal in English:
A country on the coast of West Africa; population 13,711,600 (est. 2009); capital, Dakar; languages, French (official), Wolof, and other West African languages.
- 1
- Example sentences
- He, too, took three wives - one Lebanese, one Spanish, one Senegalese.
- Plus, a rising star and a superstar, backstage with two of Africa's best as we get a taste of Ethiopian and Senegalese music.
- This well-known Senegalese dish has a particular and inimitable flavour, where lots of elements work on your tongue at the same time.
Pronunciation: /ˈˌsenəɡəˈlēz/adjective& noun
Words that rhyme with Senegalall, appal (US appall), awl, Bacall, ball, bawl, befall, Bengal, brawl, call, caul, crawl, Donegal, drawl, drywall, enthral (US enthrall), fall, forestall, gall, Galle, Gaul, hall, haul, maul, miaul, miscall, Montreal, Naipaul, Nepal, orle, pall, Paul, pawl, Saul, schorl, scrawl, seawall, shawl, small, sprawl, squall, stall, stonewall, tall, thrall, trawl, wall, waul, wherewithal, withal, yawl
Definition of Senegal in:
- British & World English dictionary
What do you find interesting about this word or phrase?
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= trending


text:
Crystal Bowersox didn't just lose American Idol this week, but her boyfriend as well. The runner-up revealed to Ryan Seacrest this morning that she split with Tony Kusian hours before her final performance—explaining her "hard morning" comment on Tuesday's show. "Big Tony, he went home. He wasn't cool with the lifestyle," she said. "I didn't break up with him, it was a mutual thing." But to paraphrase Bill Hicks, "mutual" usually means one person said "we're breaking up," and the other said "Ok"—and it sounds like Tony's the one who started it.
"It's cool though, we're both logical, grown adults," she rationalized. "He's a small town guy and that's fine, I'm a small town girl, but I want this. I want this more than anything, this career, this lifestyle. I didn't think he was up for it…he didn't think he was up for it…I was a little sad that he did it on performance day, but then again it set that fire to my belly." Kusian had already hinted at the awkwardness to People. "Some days are more fun than others, but it's been hard to find friends out there. I want to keep it low key. I don't need the attention." Hey, bud, if you're looking to avoid attention and make friends, try not to dump America's sweetheart the day of her final performance. You'll have a hard time living that one down in Toledo.
[Photo: Getty Images]


text:
|The most helpful favorable review
|The most helpful critical review
1 of 1 people found the following review helpful:
By barry from USA on 2010-10-30 08:51:54Things I liked: All metal and accurate within three inches when I installed it !
Things I would have changed:N O T H I N G
What others should know:This is the first laser sight i have ever used . Now I am a great fan of this little gem ! I installed it on a CROSMAN 1088 ( that was shooting low and right ) . Now at 28 feet i am shooting a eight shot group the size of a quarter ! This is a very easy way to make a pistol very accurate . WORTH EVERY PENNY .
0 of 0 people found the following review helpful:
By dwightja from USA on 2011-04-14 02:54:49Things I liked:The laser mounted easily to the top of my Sig Sauer air pistol and the construction appears to be solid. The small aluminum construction is conducive to the use of this laser with pistols.
Things I would have changed:When I would fire the air pistol, the laser would stop working temporarily. I tried different batteries but it still worked inconsistently. Is there a problem with the batteries (3) moving around and losing the contact? Not sure.
What others should know:If this laser worked with consistency, it would be a great buy.
|By barry from USA on 2011-01-25
|By Alyssa from USA on 2010-01-11
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Mr. Beekman Winthrop '97 will speak on "The First Ten Years of American Administration in Porto Rico" in the Living Room of the Union this evening at 8 o'clock. The lecture, which will be illustrated by lantern slides, will be open to members of the Union only.
After being graduated from Harvard in 1897, Mr. Winthrop entered the Law School, from which he was admitted to the bar in 1899. A year later he became private secretary to Mr. Taft, while governor of the Philippine Islands. From 1903 to 1904, Mr. Winthrop was judge of the Court of the First Instance in the Philippines. He was then appointed governor of Porto Rico, which position he held until 1907, when he became assistant secretary of the United States Treasury.
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I would like to get to know everyone from KY and see if we can't set up a meeting. :-) My husband is traveling to Louisville this month for a physical at the VA med ctr there. So we are relatively close to try a meeting. Anyone game?
Timeless aka Sue


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The grassy, lakeside venue at Winged Deer Park near Boone Lake was transformed Saturday into a festival where racing, recreation and relaxation were all found.
The colors of T-shirts, tents and even temporary hair dye, helped to distinguish the 37 teams signed up to compete in the 7th Annual Mountain States Dragon Boat Festival, and according to Patty Bolton, corporate director of signature events for Mountain States Health Foundation, each one brought their A game.
"The teams this year are faster than we've ever had, so the competition is really high," Bolton said. "Our ... hard work has paid off it looks like. Lots of smiles out here. Everybody's having a good time. Awareness for radiation oncology is being raised, as well as a lot of dollars, so we're excited."
Bolton said the 250-meter race has three boats in each heat. A total of 20 paddlers and one drummer man each of the dragon boats, as teams paddle as fast and as synchronized as possible through the water.
Lisa Jones, paddling with the Mahoney's Outfitters team, said the actual race, which is usually just under a minute, is really a workout. Waiting patiently to paddle again, she said she was proud of her team's first effort on the water.
"I think our first heat we were in sync, we were strong. I think everybody's having a blast and that's, of course, what's important," Jones said. "Basically, you're on the water, you're with your friends, there's good food. It's just a lot of good fellowship."
Jayme Gregory, a registered nurse with Niswonger Children's Hospital, was relaxing and playing some games with friends as her team, Pediatric Paddlers, waited for their next heat.
"We're beating the time with some cornhole and hanging out and socializing," she said. "I'm having a great time."
Gregory said the team's first heat went well, scoring them a second-place run, which she said was better than the previous times the team has competed in the festival.
"The first heat went really well," she said. "I think this year was easier. We were more prepared. Each year you do it, it gets a little bit easier."
Bolton said the atmosphere and the look of the event would remind visitors of a movie set with all of the tents, food vendors and people scattered near the water. She said around 3 p.m., though, a storm blew through the venue, knocking down many of the tents and chairs, and forcing the final runs of the Dragon Boat competition to take place earlier than expected.
The overall winner and Dragon Boat Champion team was Nuclear Propulsion, with Nuclear Fuel Services. Other winners of the Dragon Boat challenges included, The Dragon Hearts of Quillen College of Medicine, AXIS II team from Woodridge Medical, MDRowers of Mountain States infection prevention department, VAEA-Veterans Association Employee Association and Olsen's Martial Arts.
Cash Me If You Can, a team with Mountain States Health Alliance, raised the most money, totaling $20,625.
Bolton said she encourages everyone in the community to keep next year's competition in mind.
"It's just like nothing else. It's such a great little jewel that everybody needs to come take part in," she said. "It's not too early to be thinking about next year. Come in as a vendor, as a sponsor, start thinking about your team. It's time to start planning for 2013."
For information on the foundation and the Dragon Boat Festival, call the Mountain States Foundation at 302-3131.


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Related Topics
Krugman on September 11
The New York Times's Nobel laureate columnist, Paul Krugman, greeted the tenth anniversary of September 11 with a blog post contending, "The memory of 9/11 has been irrevocably poisoned; it has become an occasion for shame. And in its heart, the nation knows it."
He concluded, "I'm not going to allow comments on this post, for obvious reasons."
It's probably not worth using up one of your 20 monthly free nytimes.com page views to go look at the actual post. To me, though, almost more amazing than Professor Krugman's shame is his position on allowing comments. If he believes that "in its heart, the nation knows" that it is ashamed, why not allow comments from all those readers who want to say, "you know what, Professor Krugman, you are right, I am ashamed to be an American on September 11, thank you for voicing what I knew in my heart"? Actually, his refusal to allow comments amounts to a confession that he's wrong.
Also worth remarking upon is the arrogance on display. "I'm not going to allow comments." As if the decision on whether to allow readers the chance to react to Professor Krugman's shame rests with Professor Krugman and Professor Krugman alone. Here I am commenting regardless of whether Professor Krugman "allows" me to or not. The only thing he can control is whether some of the commenting happens on the New York Times Web site or whether it all happens somewhere else.
by Ira Stoll | Sep 12, 2011 at 12:33 pm Related Topics: New York, Paul Krugman, Press receive the latest by email: subscribe to the free futureofcapitalism.com mailing list
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Metal Gear Scanlon 4 - Part 07
Become Premium to Watch
This re-release seems unnecessary at first glance, but the conclusion of Kratos' journey serves up a level of spectacle that remains impressive in 2015.
Her Story is a bold and largely successful experiment in interactive crime fiction.
Rocksteady's gorgeous-looking third Arkham game refines its open-world format a bit and lets you drive a whole hell of a lot of Batmobile.
Game »
consists of 0 releases.
Released Nov 27, 1993
First-Person is a vantage point that attempts to simulate looking through a game character's eyes. It is most commonly found in First-Person Shooters and Racing Games, and to a lesser extent in other genres, such as RPGs and 3D Platformers.
These games are based off of an established license, such as a movie or comic. Examples are the James Bond and Spider-Man franchises.
In video games Plasma is often used as a core ingredient in weapons due to its high temperature. Due to the amount of damage it can inflict it is effective against any type of target, whether organic or metallic
An advanced form of pseudo-3D sprite-scaling, ray casting is a fundamental method for rendering simple 3D entities on a 2D coordinate plane. Objects within the coordinate plane are given specific height and width dimensions and are even mapped with a 2D texture image.
Videogames that, for better or for worse, are based on an existing non-videogame franchise. Usually drawn from movies, tie-in games have also been made about TV shows, novels, and even one-hit wonder pop bands.
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FOR IMMEDIATE RELEASE Contact: Bevin Theodore, Director of PR
Sept. 6, 2012 610-799-7797; firstname.lastname@example.com
North Whitehall Township – PPL has generously donated $10,000 to KidsPeace through a program that rewards organizations for supporting select local nonprofits.
This is the first year KidsPeace is participating in the Education Improvement Tax Credit (EITC) program, and PPL is the first company to make this special one-year commitment to our charity.
"PPL is proud to be a partner with an organization that has such a long-standing tradition of excellence in the community," said Melinda Stumpf, Regional Community Relations Director at PPL. "Making a difference in the community is at the core of what we do at PPL and this is just a small example of our dedication."
A business that makes a contribution to KidsPeace through the EITC receives a tax credit equal to 75 percent of its contribution up to a maximum of $300,000 per taxable year. The credit can be increased to 90 percent of the contribution if the business agrees to provide the same amount for two consecutive tax years.
Companies eligible for the tax credit are those who do business in Pennsylvania who are subject to one or more of the following taxes: Corporate Net Income Tax; Capital Stock Franchise Tax, Bank and Trust Company Shares Tax; Title Insurance Companies Shares Tax; Insurance Premiums Tax; Mutual Thrift Institution Tax; or Personal Income Tax of S Corporation Shareholders or Partnership Partners.
To apply for fiscal year 2012-2013, any interested business should send a one-page application for the tax credit to the Pennsylvania DCED Center for Business Financing, Tax Credit Division, Fourth Floor, Commonwealth Keystone Building, 400 North St., Harrisburg, PA 17120. Applications are approved on a first-come-first-served basis.
After the DCED reviews the application and confirms there is money available in the program, tax credits will be awarded. Once an approval letter is received, the business must make the contribution within 60 days. Upon receipt of the contribution, KidsPeace will provide substantiation of the donation, and the business simply files this with the DCED to complete the process. The business is then able to claim the tax credit on its subsequent state tax filing. Specific procedures for business owners electing to pass through the EITC to individual tax returns are found in the application packets.
For more information on how your business can participate, visit the donation section of our website or contact Patrick Slattery, KidsPeace Director of Development, at 610-799-8326 or email@example.com. You may also contact the PA DCED at 717-787-7120, email@example.com or online at www.newpa.org.
About KidsPeace
Founded in 1882, KidsPeace is dedicated to serving the behavioral and mental health needs of children, preadolescents, teens and young adults, offering a comprehensive range of residential treatment programs; accredited educational services; and a variety of foster care and community-based programs to give hope, help and healing to children, families and communities. KidsPeace offers services in Georgia, Indiana, Maine, Maryland, Minnesota, Nevada, New York, North Carolina, Pennsylvania, Virginia and the District of Columbia.


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Port Henry Ten of Essex County's 18 towns now have sole assessors. Should Moriah join them?
That's the question being asked by Tim Garrison, a Moriah town board member. Moriah now has a board of three elected assessors.
"I was approached by several residents voicing concerns of why the town has not gone to a sole assessor," Garrison said. "I informed them that was a good question and we (the town board) would look into — and if the pros out weighed the cons we would put it on the ballot and let the people of the town of Moriah decide by voting on it.
"I am suggesting that we look at going to a sole appointed assessor, not sole elected," he said.
New York State allows for three types of assessment — by an elected board of assessors, by an elected sole assessor and by a sole assessor appointed by the town board.
Several Essex County town, most recently Crown Point, have switched to a sole appointed assessor.
Garrison pointed to a Department of State study that highlights the advantages of a sole assessor. The study claims many positive reasons for a sole assessor, including lower costs, more professionally-trained assessors, the elimination of conflicts of interest and consistency.
The Moriah trustee also believes towns will be forced by the state to have sole assessors in the near future.
"Eventually we may not have a choice," Garrison said. "The state is working toward county-wide assessments. It's going to happen."
Paul Mazzotte, chairman of Moriah's board of assessors, disagrees with the notion a sole assessor is an improvement.
"When you have a sole assessor you're lucky to have that person in town one day a week," Mazzotte said, pointing out sole assessors are often shared with neighboring communities. "We (current assessors) are available seven days a week, nights, weekends. With three assessors there is always someone available to help the public."
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- Anything worth remarking upon.
- Remarkable is better, different, unique, opposed, original – to the nth degree.
- The further away you get from the standard, from the accepted, from the norm, from the status quo, from the ordinary, the more remarkable you are.
- You can be remarkably good and you can be remarkably bad.
- Remarkable doesn't mean success. Success doesn't require remarkable. But both often come hand in hand.
- Remarkable is not easy. Remarkable is not safe.
- Remarkable gathers attention to itself.
- Remarkable stands out, stands in front of, and stands apart from.
- If no one is talking about it, it isn't remarkable.
- Remarkable is only remarkable in comparison to that which is not remarkable.
- A black and white cow is not remarkable. A purple cow is remarkable. A purple cow in a field of purple cows is not remarkable. A black and white cow in a field of purple cows is remarkable.
Are you remarkable? Is your blog remarkable? Can you become remarkable? What do you find remarkable? What does remarkable mean to you?
Download a free sample from my book, Design Fundamentals.


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Tools: Using the New Sponge Tool in Pixelmator
In this short tutorial we will take a look at the Sponge Tool and how it can be used to selectively saturate and desaturate an image.
Before we begin we need to understand what saturation is. Saturation is the colorfulness or intensity of a color, increasing the saturation increases the intensity of the color. Desaturation on the other hand reduces the intensity of a color and will eventually leave a completely grayscale color.
Unlike selecting Hue and Saturation from the Image menu, the Sponge Tool allows you to paint the areas that you wish to adjust.
Selecting the Sponge Tool from the tools palette reveals our available options in the tool options bar.
- Brush - Our brush settings.
- Mode - Whether to saturate or desaturate.
- Flow - Set the strength of the brush, the higher the number the more color that is added/removed.
We will begin by increasing the saturation of the field. Select Saturate and set the Flow to 50%. Using a large brush, paint over the area you wish to saturate. Notice how the colors of the field increase in intensity.
Now let's completely desaturate the sky. Select the Desaturate option and set the Flow to 100%. Paint over the sky.
It's as simple as that! I have also adjusted the levels slightly to enhance the tone of the image.
Often, the difficult part when using the Sponge Tool is deciding how exactly to use it. Here are a few creative examples to get you started:
There we have it, a short concise look into the Sponge Tool. It really is such a simple tool and when used correctly can produce some striking results.


text: Australian actress Alyssa Sutherland invaded Vikings at the end of last season as Ragnar Lothbrok's new love interest, Princess Aslaug. Some fans still blame the beautiful royal for ruining the marriage between Ragnar (Travis Fimmel) and his first love, Lagartha (Kathryn Winnick), but the ebullient Sutherland shrugs off the haters. We chatted with her yesterday about why last night's episode, "Boneless," was the fans first chance to understand Aslaug and why if you thing Lagartha's a better woman than Aslaug then "you're not watching [Vikings] properly."


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See our latest product developments, photos and information on current work being carried out by Pro...
view more >
What is a gobo?
A gobo is best compared to a stencil or photographic slide. They are used in all manner of stage lights, automated lights and projectors. Gobos can be used to project logos, patterns or photographic images.
They can be made of metal, black and white glass or coloured glass and range in size from 22.5mm to 100mm in diameter. We have a range of adaptors to fit all types of lighting equipment and projectors.
What types of gobo are available?
Here are some photos of the different types of gobos and the projections they achieve:
Metal vs Glass
Metal gobos are the cheaper option, this is because they are made of a cheaper material and can more easily be mass produced. The drawbacks of metal gobos are:
a) Tagging
b) Only black and white images
c) Limit on detail
d) Longevity
We manufacture all our metal gobos using super-high quality brass alloy. This material has superior thermal characteristics, making our metal gobos much less prone to heat distortion and burning out than standard steel gobos.
Glass Gobos
We only use the highest quality materials. All of our glass is supplied by Beacon AB of Sweden, whose thermally robust borosilicate glass substrate coupled with high density sputtered coatings make for the best gobo material. Beacon Black Line gobos incorporate a unique dark mirror coating on the reverse side of the gobo, this drastically cuts down on internal reflections within the lamp and produces a sharper projection with much less glow or 'halation' around the projected image.
Black and white gobos
These allow for very fine detail images to be realised, even on small gobo sizes. Using the most advanced imagesetting technology available, we can produce extremely small text and images which are pin sharp. We can also produce high resolution greyscale images with fine shades and blends and photographic quality images.
Colour Glass Gobos
We use the patented Beacon Full Colour Gobo™ process. This uses ultra thin zero expansion dichroic layers producing a very thin finished gobo which will actually fit in modern automated fixtures. It also keeps all the layers of the image effectively in the same focal plane, this is particularly important in the many fixtures now available with high quality projection optics. This process uses Cyan, Magenta, Yellow and blacK (CMYK) colour mixing. This allows us to produce a very wide range of colours as well as full high definition photographic style images. We produce three main types of colour gobos:
1) Single colour, clear and black.
These allow you to have for example text and a coloured logo on the same gobo or to have a coloured background with clear and black text or graphic elements. Colour in this instance refers to flat colour and does not include gradients.
2) Two colour, clear and black
These allow you to have for example text and a coloured logo on the same gobo or to have a coloured background with clear and black text or graphic elements. Colour in this instance refers to flat colour and does not include gradients.
3) Full colour
Your imagination is the limit, we can reproduce multiple colours, shades and gradients and extremely fine detail. Complex graphics, realistic colour photographic images are all possible using this method.
Front or Rear Projection
Front Projection is when you are projecting the image from the same side as it is being viewed.
Rear Projection is when you are projecting the image from behind the projection surface and the image is therefore viewed from the other side.
Keystoning or keystone correction is required when it is not possible to position the light directly in front of the projection surface. Projecting obliquely will result in a distorted image. Projected Image can alter the artwork so that the projected image is not distorted. Contact Projected Image if you think you may require this service.
Image Calculation
If you need your projected image to be a particular size Projected Image can calculate whether it is possible to achieve and alter the image area on the gobo as required.
Artwork Requirements
We accept a variety of different formats. We use Adobe Illustrator and Photoshop CS3 to prepare artwork for gobo production.
projected image unit 17, hoults estate, walker road, newcastle upon tyne, ne6 2hl
tel: +44 (0) 191 265 9832 web: projectedimage.indigo.ws email: email@example.com