text:
"I kept being optimistic. Our training staff did a great job giving me treatment.
"We were scrimmaging. I jumped up to make a pass and landed on someone's foot. I've never had a high ankle sprain before. Normally, I can go the next day or a couple of days after."
Monthly Archives: April 2013
Clippers Coach Vinny Del Negro on Blake Griffin
"He came out in limited minutes, got a couple buckets, five assists, five rebounds, he was active but as the game went on it just got fatigued and sore and worse and worse," Clippers coach Vinny Del Negro said. "You could see him there in the third, he was struggling to move, so I just pulled the plug on that. It just didn't make sense. I wanted to protect him and make sure he was all right. But I give him a lot of credit for being out there.
"It's just unfortunate timing and a very difficult one to handle, but you have to."
Blake Griffin's ankle injury has Clippers on edge
Now the Clippers, after a franchise record 56 victories during the regular season and home-court advantage in the first round, are in trouble as Game 6 in Memphis looms Thursday.
"We've got to be desperate," said guard Chris Paul, who tied his career playoff high with 35 points, but couldn't find enough help. "That's how it is. You say playoffs don't start until somebody loses at home, I guess now it's started. We lost here at home. Tried to come out with some energy but they beat us up again."
Griffin was injured in practice Monday during a drill, when he made a layup and landed on Lamar Odom's foot. It's a high ankle sprain, the type that requires longer healing.
"It's tough, but L.O. did a great job stepping up into that spot," Paul said. "It's got to be by committee. You don't replace Blake Griffin with one person.
"It's tough, but right now we're in the heart of our season and we're fighting for our lives. BG tried to give it a go and we knew he would. It's a tough blow, but what can you do?"
Third: Grizzlies 73, Clippers 65
Third quarter: Grizzlies 73, Clippers 65.
More of the same: Griffin had to leave for the locker room again, Conley (18 points), Randolph (15 points, nine rebounds) and Gasol (13 points) remained productive and Paul was doing everything he could to keep the Clippers in the game. Paul scored 10 more points, giving him 31 going into the fourth period. However, no other Clippers were in double figures. Rebounds: Clippers 33, Grizzlies 30.
CP3 saving Clippers
Halftime: Grizzlies 54, Clippers 48
Paul's 11 points in the period, including a 3-pointer in the final minute, gave him 21 points at halftime and saved the Clippers from a disastrous quarter. Griffin retreated to the locker briefly but was back on the Clippers' bench early in the period and returned to the game with 7 minutes left. The Clippers were called for a defensive 3-second call three times in the period. Gasol's baseline spin around Griffin for a three-point play enabled Memphis to open up a 46-40 lead with Griffin picking up his third foul on the play. Rebounds: Clippers 20, Grizzlies 19.
Clippers seek more from DeAndre Jordan
The lack of production the Clippers got from center DeAndre Jordan (four points, 10 rebounds) in the two games in Memphis might not have set off alarm bells, but it was a concern heading into Game 5.
"I don't know if crossroads is the right (word) but there's definitely a level you have to play at right now that is much different," Clippers coach Vinny Del Negro said. "He has a much different responsibility now. He understands it. I haven't seen the consistency that I like, he hasn't seen the consistency he would like."
Jordan played only 17 minutes in Game 4 and took only three shots. Meanwhile, Grizzlies front-liners Zach Randolph and Marc Gasol combined for 48 points and 22 rebounds.
"I didn't see him controlling the game the way I thought he needed to and at that stage you have to go with someone you feel can," Del Negro said. "Maybe Ryan Hollins could have played a few more minutes after looking at the tape.
"But those are decisions that are made during the heat of the battle and you go with your rotation as best you can. At this stage of the game you want to stay with your rotation, but if they're not being productive, you have to look at other guys you have confidence in, that have produced throughout the series."
Jordan took four shots in the first quarter Tuesday, more than he took in either game in Memphis. Del Negro listed what he expected from Jordan.
"Defensively, offensively, being a threat out there on the glass, using his length, athleticism," Del Negro said. "But it's up to him to get out there and earn his minutes and work through anything that's thrown at him during the game."
Clippers' playoff puzzle
Each team was trying to figure why they played so well at home and struggled for the most part on the road.
They may have opposite playing styles, but few teams are as evenly matched.
"It comes down to executing well, the physicality and the intensity level," Del Negro said. "There's all different things. Why did we get them in the first couple and they turned around and got us? It's part of the playoffs.
"We've played these guys a number of times, especially last year in the playoffs, so we know each other well. It's going to come down to who can impose their will on the glass. We have to play a little bit faster tempo. We've been on the shot clock too much offensively, Gasol's zoning up a lot defensively so we've got to make him pay for that a little bit."
Clippers' Matt Barnes not mincing words
After the Clippers fell by 17 points in Game 4, in which they were a minus-17 on the boards, Clippers forward Matt Barnes was succinct in his assessment. "We got punked," he said.
That wasn't exactly met with disagreement from his coach.
"Well, when you look a the rebound differential, yeah," Del Negro said. "We've shown we've been capable doing it but you can't do it one or two games. It's a series, you've got to win four. If that's what it takes to get those guys going, that's fine. But at the end of the day the numbers speak for themselves. There can't be that big of a discrepancy and expect to win."
Tipping point for Clippers, Grizzlies
Back home for Game 5 of a series that has been controlled by the home team, the Clippers know right where they stand against Memphis tonight at Staples Center.
In fact, Coach Vinny Del Negro went as far as to agree with the proposition that the winner of tonight's game (Prime Ticket, TNT) will win the best-of-7 series.
"Probably so, yeah, it has a huge impact. This is the pivotal game," Del Negro said. "We won there last year (in the playoffs) but we can't look for another miracle, as you would say, so we have to take care of business tonight and we know that.
"In saying that, we're an excellent road team, they're an excellent road team, we know how difficult tonight's going to be and we have to lock in tonight first and control our destiny tonight with our mentality and then we'll worry about Game 6."
For the umpteenth time, Del Negro stressed the importance of rebounding, since the team that has won the board battle has won each game. The Clippers will try to return to the form that helped them control front-liners Marc Gasol and Zach Randolph in the first two games.
The Clippers listed no players on the pregame injury report. Reserve guard Keyon Dooling (strained neck muscle) is listed as questionable.
Game 6 is Thursday night in Memphis and if there were to be a Game 7, it would be Sunday at Staples Center. The times for the those two games have not yet been determined.
Clippers' Jamal Crawford on the Sixth Man award
Jamal Crawford took the news that New York's J.R. Smith was awarded the NBA's Sixth Man award Monday in stride, with Smith's groundswell coming from a late-season push from the East Coast.
"Congrats to J.R. He had a heck of a season as well," Crawford said, adding that he texted his best wishes to Smith. "There are definitely guys who are deserving. Jarrett Jack had a great season, Kevin Martin had a great season, J.R., myself as well so salute to all those guys.
"I can't say I was shocked because I saw where it was going over the last couple weeks, I guess. I look at a whole season from start to finish, but I saw what people started kind of started going with toward the end."
Crawford averaged 16.5 points per game off the bench this season and was third in the league in fourth-quarter scoring behind Kobe Bryant and Kevin Durant.
Clippers coach Vinny Del Negro pointed out Crawford's obvious talent — scoring — but ran down a laundry list of other things the 13-year veteran brings.
"Makes plays off the dribble, teams double-team him, it opens up the court for other players, makes passes off the pick-and-rolls, plays with tempo, stretches the defense," Del Negro said. "He's given me a very good effort defensively, he's given me a very good effort from day 1. All those things about him being a scorer — yeah, that's what he's known for and he's really good at it, especially when he gets going. But all the other intangibles he's been able to help us with helps us win games.
"He's been a huge catalyst for us from day 1 and the whole season. It's hard for me to look at it and say Jamal didn't deserve that. But we can't control the voting."
Crawford, who won the award in 2011 with Atlanta, was glad he could atone for his performance in Portland last season, when he averaged 13.9 points and shot only 38 percent from the field.
"To be perfectly honest., coming into the season, winning the Sixth Man or All-Star wasn't on my radar," Crawford said. "It was more kind of getting my respect back because people thought I had lost a step going to Portland. That motivated me to come in and be ready this year. That was No. 1, and helping the team as best I can."


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On June 16th 1883 children making ther way down a spiral staircase leading to the stalls from the gallery found there way barred by an unopened door. The inward opening door could not be opened because of the masses of children behind it. Of the 1100 children in the gallery a tragic 183 were to lose there lives behind the door, suffocated and crushed.The reason that they all rushed down the stairs was because gifts and sweets were being distributed from the stage.
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subset: commoncrawl


text:
Celeriac gratinMobile version Email a friend Print
On TV Tonight
- 20:00 - Chinese Food Made Easy - Spicy Sichuan
- 20:30 - Chinese Food Made Easy - Cooking for Friends and Family
- 21:00 - MasterChef: The Final Three - MasterChef
- Prep time:
- 10 min
- Cook time:
- 1 hr 10 min
- Serves:
- 6
Sophie Grigson's creamy, cheese-crusted gratin makes a fabulous vegetarian main course, and a perfect accompaniment to all manner of meats
Method1. Pre-heat the oven to 180C/ gas Mark 4. Peel and slice the celeriac and drop the slices into a large bowl of water that has been acidulated with lemon juice or vinegar. Blanch them in acidulated boiling water for 2 minutes. Drain and run under the cold tap, then dry thoroughly.
2. Butter a 25cm gratin dish. Mix a spoonful of the cream into the flour, to give a smooth paste, and then gradually work in the rest. Stir in the mustard and season with salt and pepper. Spread about one-third of the celeriac in the dish, dot with a little butter and pour over one-third of the cream mixture. Repeat twice more, until the celeriac, butter and cream are all used up.
3. Sprinkle the Parmesan over the top. Bake for an hour or so, until the celeriac is completely tender and the top browned. Serve immediately.


text:
Results 11 to 16 of 16
Senior Member
- Join Date
- Sep 2003
- Location
- Canada
- Posts
- 20,890
Posted On:
5/19/2004 10:22am
go to iron mind they make specialized gripping tools that will make your hands wrist and forearms get really strong really fast.
- Join Date
- Feb 2003
- Location
- Toronto
- Posts
- 18,608
Posted On:
5/21/2004 10:22am--
Wow, what good advice, I wonder if it has been mentioned yet on this thread??!?!Surfing Facebook at work? Spread the good word by adding us on Facebook today! https://www.facebook.com/Bullshido
Registered Member
- Join Date
- May 2004
- Posts
- 54
Posted On:
5/21/2004 9:54pm
Style: Filthy Jailhouse Tactics--
For my own forearm/wrist strength exercises, I went to a store and purchased one-gallon pickle jars. I fill them with water and basically do curls while gripping the lids of the jars with my fingertips.
Haven't been doing it religiously enough because I'm lazy, so I can't give you some awesome result like, "OMG DUDE MY WRISTS GOT HUGE IN 2 MONTHS LOL"MY NAME IS ANTAGONY I SUCK AT COMBAT SPORTS KTHX
"blahblahblah, but I don't think I'm going to train tonight."
"Well if that were true, then I'd really REALLY want to come train!"
Posted On:
5/22/2004 6:15am
Lot's of good advice so far, wrist curls and masturbation being the best. I think Ronin is is right though. Some guys are just going to have bigger wrists than other guys no matter what.
Still, this is an exercise I saw these two huge guys doing in a gym once so I started doing it and it worked pretty well for me. It's built for the forearm but it helps the wrists too. They called them "Poppeyes" .. as in Poppeye the Sailor. You hold a dumbbell in each hand and let your arms hang at your sides. Then without moving your arms you curl your wrist toward your body, then away, then back toward your body and then away. You just keep twisting your wrist back and forth. I usually do two sets of however many I can do with the same weight I use for bent over dumbbell rows. I also mix it up sometimes by twisting my wrist up and down like I'm holding a mug for a set. Play with that one and see if it works for you.


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Newport, TN Appliance Sales
Mike's Used Appliances
Mike's Used Appliances provides premier appliance sales, repair, and maintenance services to customers throughout the Newport, TN area. Whether you're looking to buy used appliances or need your appliance repaired, we're the people for you. Our experienced appliance technicians make sure every appliance we work on runs like new. We provide prompt and reliable service, so we're here when you need us most.
Learn More About Mike's Used Appliances:
- Repair, sales, and parts
- Ranges and stoves
- Refrigerators
- Freezers
- Washers
- Dryers
Contact Mike's Used Appliances today at 423-625-8741 for all of your appliance needs.
Contact Information
Hours of Business:Call to schedule an appointment!
Methods of Payment:


text:
Illuminate Your Future. TEDxSDSU will shed light on the audience through the inspiring and compelling stories of our speakers and the journey they have been on thus far.
At TEDxSDSU we:
-had 100 guests
-8 speakers including a Grammy nominated producer and cast member of the show "Survivor"
-sold out of tickets in the first week
-hosted the event in the KPBS Broadcasting Studio
-had a committee of 14 people working on the event for several months
KPBS Broadcasting Studio
5200 Campanile Drive
San Diego, CA, 92115
United States
5200 Campanile Drive
San Diego, CA, 92115
United States
- Event type:
- University (What is this?)
Speakers may not be confirmed. Check event website for more information.
Paul & Denise FejtekLess than 350 people in the world, and only a handful of married couples have ever climbed all of the Seven Summits – the highest mountain peaks on each of the seven continents. Paul & Denise Fejtek joined that elite group after reaching their seventh and final summit on May 23, 2010, the Earth's highest - Mt. Everest. Their climbs were in support of the Challenged Athletes Foundation, who also named Paul as the Most Inspirational Athlete of the Year. Paul and Denise have been featured on NBC News, Fox News, and The Today Show. They live in Newport Beach and have been named by OC Metro Magazine in their annual list of the 25 Hottest People in Orange County. Paul has balanced his outdoor pursuits over the years while working full-time as a Managing Director at the Irvine-based investment bank Hunter Wise Financial Group. He specializes in mergers & acquisitions representing owners of privately held companies in the food & beverage, oil & gas, and medical device industries among others. He completed an $89 million all cash sale of a spinal implant company in which he managed the transaction in part by satellite phone from Antarctica and later at Mt. Everest Base Camp. Denise oversees their leadership retreat company Step Outdoor Adventures, guiding corporate adventure seekers around the world.
Jeffery AdlerJeffery Adler is the founder and CEO of Dlush, a youth-market drink and treat concept. He has held the position for twelve years. The retail chain has outlets in San Diego, Los Angeles, Kuwait, and Qatar. Recent expansion initiatives include a new branch at the Rock Church in San Diego, and a Dlush branch set to premiere inside the Price Center at the University of California San Diego (UCSD) in September '13. Prior to Dlush, Jeffery worked as a retail consultant to executives of leading retail chains in Singapore, Indonesia and Thailand. Jeffery is noted for his category-defining innovations in the F&B space. In 2009 he was recognized Top 50 People to Watch, by San Diego Magazine. He has a deep passion for the F&B space, and his mission is to build a company home to San Diego that can inspire the youth to connect around his product, and from it, inspire them to pursue their own personal dreams. Dlush has been featured in a variety of publications and television programs, including Weeds (Showtime Network), Entrepreneur Magazine, Fast Company, Inc. Magazine, Chain Store Age, Nation's Restaurant News, and CNN. Jeffery is a graduate of Georgetown Law where he received his juris doctorate in international law, and University of Maryland, College Park where he graduated summa cum laude as the University's valedictorian and graduation speaker at Cole Field House. Jeffery guest speaks at schools and industry conference on topics of entrepreneurism and social change. He is very passionate about giving back to the youth of San Diego and Dlush has taken an active role in raising funds and awareness for youth-based causes. Jeffery and his wife Gilda are blessed with two beautiful children, Eric and Leah.
Robert AhdootRobert Ahdoot is the founder of YayMath.org, a free online collection of math video lessons, filmed LIVE in his high school classroom. It features authentic student-teacher interaction, fun costumes, and high quality content that's spontaneous, funny, and energetic. Since its inception four years ago, it approaches three million YouTube views, equating to over two million minutes watched. Yay Math's audience includes people of all ages and backgrounds, from over twenty countries, who have been helped by its core mission of enabling math success for everyone in the world. Milestones include an iTunes Algebra app, coverage on the news, blogs, and radio, invitations by schools to share the Yay Math message, and an address given at TEDxAJU.
Chad CrittendenChad Crittenden grew up in Half Moon Bay, California, graduated from U.C. Davis, and went on to become a bilingual educator, enjoying a 9 year career as a bilingual teacher. In the prime of his life, Chad was diagnosed with Synovial Sarcoma, a rare and deadly form of cancer, resulting in a major surgery, which saved his life. Within a year after his recovery, Chad sent in a tape to CBS' hit reality show Survivor, and was chosen to compete on season 9: Vanuatu, Islands of Fire. Chad excelled on the show, using his exposure on TV as a way of illustrating how one can overcome obstacles, and he's never looked back. During his post-Survivor years, he has been sponsored for dozens of triathlons, including XTERRA. Chad has also competed and won medals in Snowboarding and Mountain Biking, went on to complete the NYC Marathon, and in September of 2012, reached the summit of Mt. Kilimanjaro. This expedition was successful in raising significant funds and awareness for the Challenged Athletes Foundation. Chad resides in Livermore, California with his family of 4, has been cancer-free for 10 years now, and feels very fortunate for all that he has.
Maurice DiMinoMaurice is an award winning speaker, author and mentor. He created his Speaking and Presentation Template. Executives, entrepreneurs and celebrities have used his template and they have turned their Number #1 fear into their number one asset. Across the business spectrum, Maurice's clients have sold more products, converted more prospects into raving fans, and they have raised the awareness of causes and charities across America. Maurice would be the first to tell you that he has been doing public speaking all his life. He has been in the sales field for over twenty years. His last sales experience was renting and selling post production equipment in Hollywood. He was called the "Million Dollar Man", doing over a million dollars worth of business per month. He made deals with such clients as Oprah Winfrey, Dr. Phil, and "Big Brother". Already a successful public speaker, Maurice joined Toastmasters in 2004. Amazingly, that same year, Maurice was named the Number #1 speaker in Los Angeles. Maurice has studied with some of the "greats" in public speaking: T. Harv Eker, Les Brown and Tony Robbins, and many others. He has taken his years of training and experience and combined it with his public speaking skills, and added a touch of his Sicilian upbringing to produce his boot camp "Discover Your Million Dollar Message Intensive" that takes place every Spring and Fall in Los Angeles. Maurice is speaking all over the country covering topics on Public Speaking and Presentation Skills, Leadership Skills, and Success Principles. His coaching and mentoring program incorporates "The Sicilian Secret to Success," the foundational principles that help his clients to achieve results in an easy, effective and fun way.
Nadir BouhmouchBorn in Casablanca and raised in Rabat, Nadir Bouhmouch is an award-winning filmmaker, human rights activist, feminist and film/global justice student at San Diego State University. In 2011, Nadir became an active member of the Moroccan pro-democracy 'February 20th movement' which has been responsible for widespread protests, the redrafting of the Moroccan constitution and the displacement of the former Moroccan government. He is also the co-founder of the Guerrilla Cinema movement, and is particularly interested in campaigning against filmmaking regulations in Morocco which he sees as a constraint on freedom of expression. Nadir directed, produced and wrote "My Makhzen & Me" (2011). Being the first film to cover a Moroccan oppositional movement, it would become a landmark in Moroccan cinema. Nadir was hailed as the 'bete noire of Moroccan directors' by SlateAfrique Magazine, as his film, directly addressed the oppressive methodology of the Makhzen against the people, revealing issues that no other Moroccan filmmaker dared to touch. He has just recently released his first feature documentary, 475 which has been nominated by Reporters Without Borders' and the Deutsche Welle's "The Bobs" awards for Best Social Activism.
Justin AnchetaBio Coming Soon.
Organizing team
Brooke MartellTeam Coordinator
Melisa WuMarketing Co-chair
Tanisha PedenMarketing Co-chair
Alonso MojicaSocial Media Chair
Carlea LunaTeam Coordinator
Daniel NorwoodOperations Coordinator
Ambry AldayOperations Coordinator
Ashley CastroTeam Member
Chad BoyarskyTeam Member
Gabrielle JohansenTeam Member
Elle FarrellTeam Member
Pamela WattersTeam Member
Sabrina SalvatierraTeam Member


text:
A Community-Developed Dictionary of Software Weakness Types
Description Summary
Technical Impact: Varies by context
Phase: Architecture and Design
Use well vetted pseudo-random number generating algorithms with
adequate length seeds. Pseudo-random number generators can produce
predictable numbers if the generator is known and the seed can be
guessed. A 256-bit seed is a good starting point for producing a "random
enough" number.
Phases: Architecture and Design; Requirements
Strategy: Libraries or Frameworks
Use products or modules that conform to FIPS 140-2 [R.339.1] to avoid obvious entropy problems. Consult FIPS 140-2 Annex C ("Approved Random Number Generators").
This entry overlaps predictable from observable state (CWE-341).
CWE is co-sponsored by the office of Cybersecurity and Communications at the U.S. Department of Homeland Security.
This Web site is sponsored and managed by The MITRE Corporation to enable stakeholder collaboration. Copyright © 2006-2015, The MITRE Corporation. CWE, CWSS, CWRAF, and the CWE logo are trademarks of The MITRE Corporation.
Contact firstname.lastname@example.com for more information.


text:
Fiji Girl Guides Association
Girl Guiding/Girl Scouting introduced: 1924
Number of Girl Guides/Girl Scouts: 5531 (31/12/2012)
Status: Full Member
Admits boys: No
WAGGGS Region:
Asia Pacific
Contact us
Chief Commissioner
Malcolm St, 17
P.O. Box 222
Telephone: 00 6793300980
Fax: 00 6793300980
Email: firstname.lastname@example.com
Girl Guide Promise
I promise that I will do my best:
To do my duty to God
To serve the Queen and my country,
and help other people, and
To keep the Guide Law.
Brownie Guide Promise
I promise that I will do my best:
To do my duty to God,
To serve the Queen and my country,
and help other people, and
To keep the Brownie Guide Law.
Girl Guide Law
1 A Guide is loyal and can be trusted.
2 A Guide is helpful.
3 A Guide is polite and considerate.
4 A Guide is friendly and a sister to all Guides.
5 A Guide is kind to animals and respects all living things.
6 A Guide is obedient.
7 A Guide has courage and is cheerful in all difficulties.
8 A Guide makes good use of her time.
9 A Guide takes care of her possessions and those of other people.
10 A Guide is self-controlled in all she thinks, says and does.
Brownie Guide Law
A Brownie Guide thinks of others before herself and does a good turn every day.
Girl Guide Motto - Be Prepared
Brownie Guide Motto - Lend a Hand
Age groups:
Young Leaders 23-30
Ranger 17-22
Guide 11-16
Brownie Guide 7-10
Development of the movement:
The first Guide company was started in Levuka in 1924 by a Methodist missionary. In 1926 this group of Guides participated in a parade in Suva in honour of a royal visit, and as a result of this public appearance, the first Toorak Company was formed in Suva. By 1928 more companies and a Brownie pack had been established in Suva, and all companies had been registered with the Guide Association (UK). In September 1930 the Girl Guides Association of Fiji was formed.
In 1942 the activities of the growing organization were suspended when Fiji became a military base and schools were used for military purposes. At the end of the Second World War, Guiding was re-established.
Over the years, trainers and other experienced personnel from the Guide Association (UK) have visited Fiji to assist the Association in the development of its programme, and the implementation of its training strategy.
In 1980 the Guide Association (UK) signed a Deed of Transfer giving the Fiji Girl Guides Association self-government in all areas of work.
The programme used in Fiji was prepared by the South Pacific Guide Conference for particular use in the Pacific Islands. Members with special needs are integrated into ordinary units whenever possible. In 1998, at the workshop in Nadi, Fiji, Leaders and Trainers of the South Pacific prepared the new Pacific Islands Girls Programme for the three sections. These new programmes are now used in the Islands.
Relationship to society:
Many Guides work in hospitals caring for patients. All age groups participate in community service projects during the annual Guide and Scout Week when specific projects are carried out. Members also collect firewood and clear gardens for the elderly.
Rangers are involved in a variety of income-generating projects, including sewing and vegetable gardening. In urban areas, some units learn office skills such as typing, book-keeping and office-management.
Communication and Co-operation
Fiji is a member of the Caribbean Link set up for the purpose of consultation and mutual aid between Guide Associations in Commonwealth countries and Caribbean Islands. Members participate in international events.
The Association is recognized by the Government through the Education Department, the Ministry of Youth and Sport and the Ministry of Women. The Guides are an important part of community life, and are often invited to participate in national days and other events.
Every month a 15-minute radio programme on Guiding is broadcast in Fijian, Hindi and English and the Association publicizes its activities through its periodical, Fiji Newsletter.
The Fiji Girl Guides Association combines with the Fiji Scout Association each year to hold a Guide and Scout Week. The Association also works with organizations such as the YWCA, the Red Cross and the St. John Ambulance Association, and is a member of the Fiji National Council of Women.
Fiji Girl Guides Association is now embarked on a membership drive through its training and communities programmes and is endeavouring to recruit members who left the Association during the 2000/2001 upheavals.
A Training Adviser, who heads a training team of 12, has the responsibility for implementing the training programme and supervising all training sessions held in the country. Local and national training events are arranged at regular intervals.
Fiji has now 28 certificated trainers holding the Pacific Bronze Badge. Five hold the Pacific Silver Badge.
Outdoor and Environmental Activities
Residential courses are to be introduced at a Training Centre and campsite near Suva. The interest that Guides in Fiji take in their environment is demonstrated by their efforts to clear public parks and footpaths and to plant trees.


text:
The second petition to flood Holmes Field for a skating pond was refused by the Corporation at their last meeting. With this final decision the project will have to be abandoned.
While no definite statement has been made by the Corporation, it is understood that they opposed the petition unanimously. The chief objections were: the late time of the year for beginning a scheme which would be essentially experimental; the liability to injure the field by embankments for the pond, and by the extensive drainage required; the liability to kill the grass by so much ice; the apparently deficient estimate of the expense of the work; and the inconvenience to those who have to cross the field. The Corporation has never been in favor of enclosures where admission is paid, and they felt that a skating pond on Holmes Field, run like a common rink, would be sadly out of harmony with the surroundings.
Want to keep up with breaking news? Get the latest, straight to your inbox.


text:
Newsletter #450
January 30, 2012
Forgot to make plans for Valentine's Day? ?InLiquid is giving you three chances to celebrate with art: Second Thursday & Young Professionals Preview on February 9, Benefit v.12 on February 10, and?Anti-Valentine's Day Party?on February 14 (in partnership with the Food Trust). ?Online bidding?for InLiquid Benefit v.12 is now open, so you still have a chance to snag last-minute gifts as well. ?Tickets on sale now to all of the events!
Join InLiquid! Our next membership deadline is February 28.
SAVE THE DATE! Art for the Cash Poor 13 will be on June 9 and 10.
You can keep track of upcoming events like the ones above by purchasing the InLiquid 2012 Calendar.
And be sure to spread the love by voting for the Clay Studio's Claymobile, which brings ceramic arts education to diverse populations, and help them reach their goal of winning $5,000 in gas.


text:
Zero Waste Events
The Zero Waste How-To Guide was developed by the Green Campus Partnership to help student, faculty and staff in the Penn community achieve zero waste status at events ranging from department-wide gatherings to small staff meetings, staff picnics to lunchtime seminars.
Using the Zero Waste How-To Guide and our customizable signage (available at the bottom of this page), any campus event can contribute to the University's Climate Action Plan goal of minimizing waste and decreasing our carbon footprint.
Green Campus Partnership wants to know about the zero waste events held on campus! Fill out this form after hosting a zero waste event to fill us in on your experience.
Check out our list of Zero Waste Caterers that can help make your event zero waste.
Zero Waste in Action…
Several events on campus have already achieved success as zero waste events. Read about their accomplishments and apply their organizational strategies to your next event!
February 26, 2013
November 9, 2013
Wharton Staff Appreciation Event
November 6, 2013
FAQs about composting
What should be composted?
- All food waste, wooden coffee stirrers, compostable cups, bowls, plates and utensils, napkins, paper towels, tea bags and coffee grinds, compostable garbage bags
What shouldn't be composted?
- Styrofoam, plastic bags, metal, plastics, foil, sugar packets, individual ketchup packets or similar sauce packets
Where do I get a compost bin?
- You can repurpose an existing waste container as a compost bin by using a compostable bag liner and clearly marking the content with signage
How do I know if my materials are all compostable?
- Biodegradable Products Institute: certifies specific products as guaranteed compostable. Check to see if the materials you plan on using are BPI certified!
Zero Waste Event Signage
Prominent signage is key to hosting a zero-waste event. Using the zero-waste event graphic and labeling compost, trash, and recycling bins with the posters below can help make your event a success.Download our customizable signage for your Zero Waste Event!
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|Friday, 08 February 2013 15:45
February, 1973
February 1 – Capital Hill News – Mr. and Mrs. Leo Berry spent last Monday with their sister, Mrs. Neville Cunningham. Her visitors Tuesday were Mr. and Mrs. G H Cunningham. Mrs. Vera Pennington of Indianapolis spent the weekend with Mr. and Mrs. Lexie Wyatt of Glasgow.
AD – Hardees Hamburgers opening soon in the Happy Valley Shopping center.
Rt. 5 Newsletter by Mrs. Renna Matthews – Mr. and Mrs. Wilbur Bewley spent Wed. with his parents, Mr. and Mrs. J A Bewley. Mr. David Matthews visited Mr. and Mrs. Otha Rigsby awhile Sunday morning. Mrs. Donald Matthews and Tracy were visiting Mrs. David Underwood and children awhile Friday night.
February 8 – Recent births: Robert Bradley Cecil, Merrill Smith Simmons, Ann Harwood Jolly, and Tonya Kaye Bowles.
Taylor Tales by Stanton Taylor – Mrs. Dewey Botts, Bowling Green has returned to her home after spending several days with her daughter, Mrs. Harold Warren, Cincinnati.
Miss Marcia Compton, a sophomore at Hiseville High School was crowned 1973 Homecoming Queen by last year's queen, Rebecca Johnson. Hiseville clipped Clinton Co. for the win.
February 15, 1973 – Ken Scott, Glasgow Junior High School Principal is the Red Cross Fund Chairman for 1973.
Temple Hill Accomplishments by Sue Jones – PTA was held in school library. A skit was presented called "wanted, a Husband"/ Those in the play were Roger Barrick as Deacon Dudley the wanted husband, Janet Payne as Merandy the old maid, Vickie Vance as Peggy, a tomboy and Rodney Pace, a book salesman.
Mason Dixon Games participants are Kim Davidson, April Bull, Cheryl Smith and John Stuart.
Edmonton News by Jasper Linconn – The Elroy Shirleys of Glasgow had supper Friday night with the Amos Braggs of Prices Creek. The Braggs and the Johnny Wilsons had supper the night before with the Fred Dicksons.
Little League Champs – Mark Parker, Kevin Waldrop, Kendall Bowles, Anthony Smith, Jeff Harp, Rodney Huff, Mark Kerly, Mark Bowles, and Coaches Roger Martin and Steve James.
Pee Wee Champs – Rusty Sally, Burl Scott, Junior Daniel Watson, Mark Bellamy, David Morrison, Craig Hampton, Paul Lewis, and Coach Clyde Salley.
The 7 new board members of the Barren Co. Conservation District are Jessie Morgan, Joe Donald Taylor, Mitchell Bertram, Felix Murray, Jewell Colliver, and B H Deweese. James Chamberlain was not present.
Lawrence Tidrow, US District Soil Conservationist was the guest speaker for the Glasgow Garden Club. Mrs. C G Follis, is the President.
Hiseville News – Miss Liz Alexander, Lindsey Wilson College, was house guest from Thursday until Sunday of Mr. and Mrs. Mitchell Bunch. On Sunday, Liz and Bruce Bunch were dinner guests of her grandparents, Mr. and Mrs. J B Alexander.
February 22, 1973 – American Music month was officially proclaimed in Glasgow this month by Mayor Luska J Twyman. Shown with Mayor Twyman are Mrs. David Depp and Mrs. C R Richardson.
Scottie Chatter by Lynell Chamberlain – On Thursday, Feb. 15, the Senior girls ordered their corsages for the Senior night game. The corsage will be of daises with navy blue and burgundy streamers. The Scottie team will be playing the Hart Co. team that night.
City Auto Electric, Auto Parts business located on N. Broadway was gutted by fire Tuesday night with an estimated loss of $100,000. City Auto was operated by Foch Elliott.
Cumberland Parkway officially opens on February 28.
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A microbubble-type ultrasonic imaging agent is provided comprising a parenterally-administerable aqueous medium containing a dispersion of microspheres predominantly of diameters less than 10 microns, wherein the microspheres consist of gas microbubbles encapsulated with water-insolubilized biocompatible material. The imaging agent is characterized by having a concentration of greater than 100×106 microspheres per milliliter, and a stability such that this concentration is maintained for over 4 weeks at a temperature of 20° to 25° C.
1. A concentrated, storable ultrasonic imaging agent, comprising an aqueous parenteral medium containing a dispersion of microspheres predominantly of diameters less than 10 microns, said microspheres consisting of gas microbubbles enclosed by solid walls formed from heat-insolubilized biocompatible material, said imaging agent having a homogeneously dispersed concentration of greater than 1×108 microspheres per milliliter, said microspheres being stable in said medium as evidenced by maintaining a concentration of greater than 1×108 microspheres per milliliter for over 4 weeks at a temperature of 20° to 25° C.
2. The imaging agent of claim 1 in which said biocompatible material is a heat sensitive protein.
3. The imaging agent of claims 1 or 2 in which at least 80% of said microspheres have diameters in the range from 1 to 9 microns.
4. The imaging agent of claim 1 in which said biocompatible material is human serum albumin.
5. The imaging agent of claims 1 or 4 in which said gas is air.
6. A concentrated, storable ultrasonic imaging agent comprising an aqueous parenteral medium containing a dispersion of microspheres at least 80% of which have diameters in the range of 1 to 9 microns, said microspheres consisting of gas microbubbles enclosed by solid walls formed from heat-insolubilized protein, said imaging agent having a homogeneously dispersed concentration of greater than 1×108 microspheres per milliliter, said microspheres being stabilized in said medium as evidenced by maintaining a concentration of greater than 1×108 microspheres per milliliter for over 4 weeks at a temperature of 20° to 25° C.
7. The imaging agent of claim 6 in which said protein is human serum albumin and said gas is air.
8. The imaging agent of claims 6 or 7 in which said medium is an aqueous solution of the protein of the microsphere walls.
This invention relates to ultrasonic imaging of the human body for diagnostic purposes; and, more particularly, to ultrasonic imaging agents.
It has been known since 1968-70 that contrast echocardiography can be used to delineate intracardiac structures, assess valvular competence, demonstrate intracardiac shunts, and identify pericardial effusion. (Gramiak and Shah, 1968; and Feigenbaum, et al., 1970.) Ultrasonic imaging of the heart potentially has important advantages of convenience, safety, and reduced cost over present diagnostic procedures, such as angiography, which requires the use of radio-opaque dyes for X-ray imaging, or the use of radio-nuclide imaging agents for radio-imaging. However, progress in practical applications of ultrasonic imaging has been delayed by the lack of effective clinically-usable imaging agents.
Ultrasonic imaging utilizes an ultrasonic scanner to generate and receive sound waves. The scanner is placed on a body surface overlying the area to be imaged, and sound waves are directed toward that area. The scanner detects reflected sound waves and translates that data into images. When ultrasonic energy is transmitted through a substance, the acoustic properties of the substance depend upon the velocity of the transmission and the density of the substance. Changes in the substance's acoustic properties (e.g., variations in acoustic impedance) are most prominent at the interfaces of different substances, such as a liquid-solid or liquid-gas interface. Consequently, when ultrasonic energy is directed through media, changes in acoustic properties will result in more intense sound reflection signals for detection by the ultrasonic scanner.
Ultrasonic imaging agents can consist of small solid or gaseous particles which, when injected in the circulatory system, provide improved sound reflection and image clarity. Microbubble-type imaging agents consist of minute bubbles of a gas (usually air) which are dispersed in a carrier liquid for parenteral injection. The "microbubbles" are carried by the circulatory system to the organ being imaged.
It has been proposed to form a dispersion of air microbubbles in a warm aqueous gelatin solution, and cooling the solution to a solidification temperature to trap the microbubbles. For administration, the gelled dispersion is to be warmed until it liquifies, and parenterally administered with the microbubbles dispersed in the liquified gelatin. (Tickner, et al. U.S. Pat. No. 4,276,885;- and Tickner, et al., National Technical Information Service Report HR62917-1A, April, 1977).
Gelatin-trapped microbubbles on introduction into the bloodstream have a short life-time. They rapidly dissipate. Another disadvantage is that the microbubbles are too large to pass through capillary beds, and are therefore not suitable for heart imaging by peripheral intravenous administration.
The discovery by Dr. Steven B. Feinstein of sonication-produced microbubble imaging agents represented an important advance in this art. Using viscous aqueous solutions, such as 70% sorbitol or dextrose, Dr. Feinstein produced a dispersion of microbubbles by high energy sonication of the solutions. The resulting microbubbles had sizes less than 10 microns, and were capable of passing through capillary beds. The persistence of the microbubbles, although of the order of a few minutes, permitted the imaging agent to be pre pared and administered intravenously for heart imaging. (Feinstein, et al., 1984; and Feinstein U.S. Pat. No. 4,572,203.)
Subsequently, Dr. Feinstein sought to improve the persistence of the microbubbles. He found that by sonication of a heat-sensitive protein, such as albumin, microbubbles of improved stability were obtained. (See Feinstein, PCT Application WO No. 84/02838, corresponding to allowed U.S. application Ser. No. 805,975, filed Dec. 5, 1985, now U.S. Pat. No. 4,718,433). Concentrations of microbubbles of 10 to 14×106 microbubbles per milliliter were obtained with bubble sizes from 2 to 9 microns (Keller, Feinstein, and Watson, 1987). The microbubbles persisted for 24 to 48 hours.
However, the sonication-produced albumin microbubble imaging agent of Feinstein was not sufficiently stable for commercial manufacture. Stabilities of the order of weeks or months (rather than hours or days) are required to permit an imaging agent to be manufactured at a central location and distributed to hospitals in the United States and other countries. For commercially feasible manufacture, shipment and hospital storage prior to use, a stability time of at least four weeks is needed and preferably at least eight weeks or longer.
Further, for the most effective imaging, it is desirable to have the highest obtainable concentration of microbubbles in the imaging agent. But the population of microbubbles of the desired small sizes tends to decrease with holding of the sonicated albumin solutions. The small bubble size attrition can occur either by collapse of the microbubbles, or by coalesence to oversize microbubbles. Consequently a further important objective has been to find means for increasing concentrations of microbubbles in the imaging agent. An imaging agent of very high microbubble concentration is inherently better, and a safety factor is provided. With a concentration of microbubbles higher than the minimum required for effective imaging, some loss of the microbubbles of the desired size can be accepted.
The present invention provides an ultra-concentrated, room-temperature stable microbubble-type imaging agent. This improved imaging agent comprises a sterile aqueous medium containing a dispersion of microspheres predominantly of diameters less than 10 microns, and is thereby suitable for parenteral intravenous administration. The microspheres consist of gas microbubbles encapsulated in a water-insolubilized biocompatible material, such as albumin. Representing a substantial advance in the art, the imaging agent of this invention has a homogeneously dispersed concentration of greater than 100×106 (e.g., 108) microspheres per milliliter. This high concentration can be maintained at ordinary room temperatures (20° to 25° C.) for extended periods of time (4 to 8 weeks or longer). In optimized embodiments, microsphere concentrations of the order of 300 to 500×106 microspheres per milliliter are achieved. Surprisingly, these ultra-high concentrations can be maintained for over eight weeks. The imaging agents of this invention are therefore adapted for manufacture and distribution on a commercial basis. Following shipment, they may be maintained in inventory by hospitals for many weeks, being available for diagnostic use as required.
The imaging agents of this invention are preferably produced from a heat-denaturable biocompatible protein by a stepwise sonication procedure. As with the Feinstein method, an aqueous solution of protein is subjected to sonication to form gas microbubbles while concurrently heating the solution to insolubilize small portions of the protein. However, the improved sonication procedure, which results in the increased concentration of highly stable microbubbles utilizes a novel sequential sonication. In the initial sonication phase, the sonicator horn is directly contacted with the solution (viz. by immersion just below the upper surface of the solution). This initial sonication is carried out without appreciable foaming of the solution. In the next phase of the sonication, foaming is promoted. The sonicator horn is withdrawn to a position in the ambient atmosphere above but proximate to the surface of the solution. Intense foaming and aerosolating occurs. The population of microbubbles is thereby greatly increased and the microbubbles are encapsulated with denatured protein to obtain a dispersion of highly stable microspheres. Moreover, the stability of the microspheres permits them to be concentrated and/or fractionated. By such manipulations, bubble concentration can be doubled or tripled and oversize bubbles eliminated.
For example, the concentration of the microspheres as initially produced can be from 50 to 150×106 By a float separation concentration procedure, the microsphere concentration can be increased 200 to 600×106 microspheres per milliliter. Also, by another float-type separation, most of the microbubbles of larger size than 10 microns can be removed, resulting in an imaging agent composed predominantly of microspheres of diameters substantially less than 10 microns. For example, at least 80% of the microspheres can have diameters in the range from 1 to 9 microns.
The accompanying drawings illustrate a preferred method of preparing the ultrasound imaging agent of this invention.
FIGS. 1A to 1D illustrate the steps in the sequential sonication procedure.
FIG. 2 is a cross-sectional view taken on line 2--2 of FIG. 1B, illustrating the relation of the sonicator horn to the inside of the syringe which contains the albumin solution being sonicated.
FIG. 3 illustrates a separator vessel in which increments of the microsphere dispersions are pooled for float separation concentration.
FIGS. 4, 4A, and 4B illustrate a method of fractionation of microsphere dispersions to remove oversize microspheres.
FIG. 5 is a graph of experimental data showing the concentration of the microspheres in the imaging agent as produced, and their storage stability.
The starting material for practicing this invention is an aqueous solution of a suitable biocompatible material. The encapsulating material should be heat-sensitive so that it can be partially insolubilized by heating during sonication. More specifically, coincident with the sonication, a small portion of the dissolved biocompatible material is heated or otherwise treated so that its solubility is reduced. This results in a small volume of solid phase material, which forms the encapsulating layers around the microspheres. Preferably a heat-sensitive protein is selected such as albumin, hemoglobin, collagen, etc. For administration to humans, human protein is preferred. Human serum albumin (HSA) is especially suitable. HSA is available commercially as a sterile 5% aqueous solution, which can be used directly as the starting material for preparing the microspheres. However, other concentrations of albumin or other heat-denaturable proteins can be used. HSA concentration can be varied, for example, within the range from 1 to 25% by weight.
Commercially-available sonicator equipment may be used in practicing this invention. Theoretically, sonicator vibration frequencies can vary over a considerable range, such as from 5 to 30 kiloHerz (kHz), but most commercially-available sonicators operate at 20 kHz or 10 kHz. The 20 kHz sonicators perform well for purpose of this invention. Such sonicator equipment can be obtained from Heat Systems-Ultrasonics, Inc., Farmingdale, N.Y., and other companies. Ultrasonics Model W-380 or similar model can be used with a flat tip, high gain sonicator horn. The power applied to the sonicator horn can be varied over power settings scaled from 1 to 10 by the manufacturer, as with Ultrasonics Model W-380. An intermediate power setting can be used (viz. from 4 to 8). The vibrational frequency and the power applied must be sufficient to produce cavitation in the liquid being sonicated.
The solution to be sonicated can be treated in small increments. For example, 8 ml. quantities of the solution can be individually sonicated. Initial sonication can be carried out with the flat-ended sonicator horn in contact with the solution, preferably immersed in the upper portion of the solution. Immersion is desirable in order to carry out the initial sonication without appreciable foaming. With a power setting of 4 to 6, the initial sonication can be performed in less than a minute (viz. 15 to 45 seconds).
Immediately following the initial phase of the sonication, the sonicator horn is withdrawn to a position above the solution but proximate to the upper surface of the solution. In the second phase, the sonication is deliberately carried out in such manner as to produce intense foaming of the solution, contrary to conventional sonications, where it is desirable to avoid foaming. For the purpose of the present invention, foaming and aerosolating are important for obtaining the imaging agent of enhanced concentration and stability.
To promote foaming, the power input to the sonicator horn may be increased in the second stage. For example, the power setting may be moved from an initial setting of 4 to a setting of 6. The second phase of the sonication can be carried out in less than a minute, (viz. from 15 to 45 seconds). The total time for the sonication for both the first and second phases can be of the order of one minute. For example, a 25 to 35 second sonication can be used for each phase. The foaming produced in the second phase of the sonication is immediately detectable by the cloudy appearance of the solution, and by the foam produced.
By means of the sequential sonication, comprising the cavitation phase followed by a foaming phase, the concentration of the encapsulated microbubbles, referred to herein as "microspheres", can be greatly increased. Concentrations in excess of 25×106 microspheres per milliliter are easily obtainable, such as from 50 to 150×106 concentrations. Moreover, the resulting microspheres will be predominantly of diameters less than 10 microns. For example, 80% or more of the microspheres can have diameters in the range from 1 to 9 microns with a mean diameter of 4 to 6 microns.
When the sonication is carried out in contact with air as the ambient atmosphere, the microspheres will have air centers. Air is believed to be the most convenient ambient atmosphere, but, if desired, sonication could be carried out under other gas atmospheres (viz. nitrogen, oxygen, carbon dioxide, etc.).
Following initial production, the microsphere dispersions can be further processed to increase the concentration and/or to remove oversize microspheres. Since the microspheres are buoyant they tend to rise to the surface of the dispersion. By holding the dispersion without agitation for a number of hours, (viz. for 4 to 12 hours), most of the microspheres will rise to the surface and concentrate in an upper layer above the clarified solution. By this "float-separation" of the microspheres into an upper layer, portions of the clarified solution can be removed from below the microspheres, thereby obtaining a dispersion of greater microsphere concentration. For example, from 50 to 75% of the solution volume may be removed in this concentration process.
Either before or after the above-described concentration, float-separation of oversized microspheres can be obtained. Large size microspheres such as one having diameters greater than 10 microns have relatively greater buoyancy. They will therefore rise more rapidly to the surface of the solution. By utilizing a short holding time, such as from 15 to 45 minutes, the largest size microspheres can be selectively collected in a small upper layer above a dispersion which will still contain substantially all of the microspheres of small size. By removing this microsphere dispersion from beneath the layer of oversize microspheres, a fractionation may be achieved in which the larger microspheres will remain in the vessel in which the fractionation is carried out.
The imaging agent produced by this combination of two-stage sonication and the float-separation concentration can have a homogeneously-dispersed concentration of greater than 300×106, such as from 300 to 900×106 (3 to 9×108) microspheres per milliliter. High concentrations can be maintained for long periods of holding at ambient room temperatures (20°-25° C.). Concentrations above 200 and typically above 300×106 microspheres per milliliter can be maintained for periods of at least four and usually eight weeks or longer.
In FIG. 1A, there is shown a 10 ml syringe having an open top and a stopcock-type valve at its lower discharge end. The syringe is filled to the 8 ml level with the 5% albumin (HSA) solution. The sonicator horn is inserted in the syringe to the 7 ml level, indicated as the T1 position in FIG. 1B. In this position, the sonicator horn is immersed in the upper portion of the solution, the solution level being as indicated in FIG. 1B. Initial sonication is carried out essentially without foaming of the solution.
Immediately following initial sonication and without turning off the sonicator, the horn is withdrawn to the 10 ml level, indicated as the T2 position in FIG. 1C. The power input to the sonicator horn can also be increased as it is withdrawn to the T2 position. Immediately following the withdrawal, foaming of the albumin solution commences and the solution becomes milky in appearance. The solution will foam upwardly around the sonicator horn during the second phase. The appearance of the foamed solution is illustrated in FIG. 1D, the microbubbles being indicated in greatly enlarged diameter over their actual micron range sizes.
The solution being sonicated contains both dissolved and entrained air. The solution is in contact with the ambient atmosphere around the sonicator horn. (The clearance between the horn and the inside of the syringe can be seen in the cross-sectional view of FIG. 2.) The air contact facilitates the foaming and aerosolating of the solution in the second stage of the sonication.
The dispersions from a plurality of sonication batches can be pooled for concentration. For example, a plurality of the dispersion increments can be introduced into a separator vessel, which may be a large syringe or separator funnel equipped at its bottom with an outlet controlled by a drainage valve. Such a separate vessel in the form of a large syringe is shown in FIG. 3. By holding the pooled dispersions for several hours without agitation, such as overnight holding, the microspheres will rise to the top of the solution and form a layer of float-separated microspheres. Beneath the collected layer, the clarified albumin solution will be substantially free of microspheres. It is therefore possible to drain off a major portion of the solution through the bottom outlet. For example, one-half to three-fourths of the solution can be removed. However, it is desirable to retain a sufficient solution volume to permit full redispersion of the concentrated microspheres.
In FIG. 4 illustrates the microsphere concentrate with the microspheres redispersed. The microspheres are sufficiently stable that they do not adhere permanently to each other in a concentrated layer, remaining as separate intact microspheres. They can readily be redispersed by mild agitation.
After redispersion to an essentially homogeneous condition, fractionation may be carried out to remove oversize microspheres. By holding the redispersion for a short time, such as around 30 minutes, the largest diameter microspheres will preferentially rise to the top and collect in a layer, as indicated in FIG. 4A. When that has occurred, the microsphere dispersion beneath the oversize microspheres can be removed through the drainage valve. When the collected oversize microspheres approach the valve, the valve is closed so that the oversize layer remains in the separator vessel, as indicated in FIG. 4B. The product obtained is a concentrated fractionated albumin microsphere product in which at least 80% of the microspheres have diameters in the range from 1 to 9 microns. The preferred product has at least 90% of the microspheres with diameters of from 2 to 8 microns.
Further directional details of the presently preferred procedures are set out below under the appropriate headings.
Fill a 10 ml syringe of oval cross-section fitted at its lower outlet end with a stopcock to the 8 ml mark with sterile 5% human serum albumin. Position a sonicator probe of smaller cross-section in the syringe so that the bottom of the probe is at the 7 ml mark. Sonicate at energy setting 6 for 30 seconds then (with the sonicator still on) move the probe tip to the 10 ml mark, while moving the energy setting to 8. Sonicate for an additional 25 seconds. Turn off sonicator, remove probe and drain contents of the syringe into a 60 ml syringe or separatory funnel with a stopcock controlled bottom outlet. From 5 to 6 syringe volumes are pooled.
Allow the pooled increments to stand overnight (8-12 hours) without agitation in the separator vessel. When substantially all the microspheres have formed a layer on the top, drain two-thirds of the volume from the bottom.
Resuspend the microspheres and fill a 60 ml syringe with them. Let sit 30 minutes, then drain all but about the last 3-4 ml into a collection vessel. The oversize microspheres are left. Count a sample and calculate the concentration, mean diameter, and percentage less than 10 μ. If less than 99.5% are less than 10μ, re-fractionate. If required for redispersion, concentration may be adjusted with 5% HSA.
Concentration measurements are set out below in Table A for three representative runs using the procedures described above. The initial concentration of the disperions after sonication was of the order of 130 to 140×106 /ml. This was increased by the float-separation concentration to 340 to 450×106 /ml.
For product control, the microspheres may be counted by a Coulter Counter, obtainable from Coulter Electronics, Inc., Highleah, Fla. (viz. Coulter Counter Model TAII). Microsphere counts set out above were determined in this way.
The stability of a representative product was examined in a study lasting for 20 weeks. The initial concentration was approximately 4.31×108 (431×106) microspheres per milliliter. Concentration measurements were made at about weekly intervals. The results are summarized in Table B. The measurements, which were made by means of a Coulter Counter, are presented graphically in FIG. 5. The samples were held at ambient room temperature (20°-25° C.). The concentration of about 400×106 microspheres per milliliter was maintained for 20 weeks. This evidences a high degree of room temperature stability.
The stability of the microspheres can be affected by unusually hot or cold temperatures. However, even at temperatures as low as 4° C. or as high as 37° C., microsphere concentrations in excess of 200×106 /ml can be maintained for periods of eight weeks or longer. Nevertheless, for commercial distribution or long-term holding very high or low temperatures should be avoided. Room temperature holding is preferred. Temperature protection of the microspheres during shipment can be used.
TABLE A______________________________________Concentration Measurements Microspheres/ml Microspheres/mlRuns After Sonication After Concentration______________________________________A 135 × 106 386 × 106B 141 × 106 483 × 106C 133 × 106 440 × 106______________________________________
TABLE B______________________________________ MicrosphereWeek Concentration × 108______________________________________0 4.311 4.492 4.203 3.914 3.865 4.256 4.067 4.128 3.929 3.9410 3.9711 3.4812 3.4813 4.0914 3.7015 4.9217 4.1518 3.9919 4.14______________________________________
Feigenbaum, et al. (1970), Circulation 41:615-621
Feinstein U.S. Pat. No. 4,572,203.
Feinstein PCT Application WO No. 84/02838.
Feinstein, et al. (1984), J. Am. Coll. Cardiol. 3:14-20.
Gramiak and S
Tickner et al. U.S. Pat. No. 4,276,885.
Tickner et al., National Technical Information Service Report HR 62917-1A, April, 1977, pages 34-40.
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Submit your article and become a guest writer.
Here at Bodybuilding Warehouse, our active customer communication is our most important feature and our 81,000+ strong Facebook community is always alive with activity. The basis of a lot of the discussions on our social sites is the content that you find right here on our fitness blog.
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Hey I got a little problem in unity with my sketchup model. I use sketchup pro 8.. When I export the sketchup model and then put it in unity the model looks really dark? It looks great in sketchup but in unity the model is really dark even if I add a lot of light.
The model are almost so dark that i can't see my texture? is there any way i can fix this without having to light up every side of my model? I am only able to see it like it should if I light up every side of the model, but I really don't wanna do that on every single model I use in my game I know you can get plugins for sketchup like Lightup and Vray but really don't wanna spend money on it if theres another way to fix it.
asked Dec 16, 2010 at 10:45 AM
edited Aug 06, 2011 at 08:01 AM
Maybe your Scene ambient color is to dark. You can change it under Edit>> Render Settings>> Ambient Light.
answered Dec 16, 2010 at 12:19 PM
Try changing the colour property of the material in Unity (I'm assuming it's a Diffuse shader) to white.
answered Dec 16, 2010 at 11:11 AM
Novodantis 1
I second this, I always use sketchup and this is what you do.
Dec 28, 2010 at 10:17 PM Fishman92
My scene ambient light was set to pure black, so that was maybe why. I just took it all up to white. I can see my model a lot better now! Gonna see how it all works out when I add more models to my scene.. But I think that the problem is solved untill now. So I'm just gonna wait and see till I've made some more models for my game.
Is there any other way to fix the problem? I'm just curious"^^'.
And again thanks for helping me out!
answered Dec 16, 2010 at 05:38 PM
you need to light map the sketchup model in Unity, it took me ages to work that one out.
check out the Beast Lightmapping Tutorial
answered Dec 16, 2010 at 08:34 PM
Jono 2
proably because you forgot to import the model with matrials
answered Dec 28, 2010 at 09:47 PM


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Clerics live it up in Vatican for Law's 80th
Cardinal Bernard Law was treated to a lavish birthday spread, the company of a conclave of clerics and even the music of a mariachi band in a four-star Italian hotel, where guests rolled up in Vatican Mercedes sedans and left singing the praises of the fallen prelate promoted to his Holy City post after decades of covering up clergy sex abuse in Boston.
The resplendent reception that marked Law's 80th birthday sent shock waves an ocean away in Boston, where the mere mention of his name still sparks seething anger in clergy abuse victims whose attackers he protected during his years as archbishop.
"He's closing in on his remaining years, and as a man of faith I know he believes in a final judgment. I'm curious if he's getting nervous," said Phil Saviano, who was abused by a Worcester priest almost five decades ago.
"I wonder if he looks back on all the good he accomplished and if he thinks that … outweighs so many hundreds of kids on his watch who had to go through so much trauma and emotional pain," added Saviano, who founded a local chapter of Survivors' Network of those Abused by Priests.
The Herald revealed yesterday that Boston Archbishop Cardinal Sean O'Malley, in Rome on church business, had snubbed Law's Vatican birthday shindig.
Yesterday, an archdiocese spokesman issued a statement saying O'Malley "does extend his prayers to Cardinal Law on the occasion of his 80th birthday." With a pair of guards in colorful habits standing silent sentry at the gate, Law and his cloistered concelebrants wined and dined at the Al Chiostro restaurant in the four-star Palazzo Rospigliosi hotel facing the Basilica of St. Mary Major, where Law serves as archpriest. [more]
12 Comments
Isn't it time we stopped donating to these clowns? Ill give to the St Vincent de Paul Society and/or directly to a poor person.
The church is a caste system. The Bishops are mini Kings over the serfs, the people in their domain. Pray, pay, obay. They dress in their red robes, only the best,They have futher insulted the people of America first and the world by celebrating this mans birthday. What a sad day for the American church. "I have come to serve and not to be served." They have no idea what this means."
What a pity that the Vatican is so totally insensitive to the feelings of the American laity that is appalled by Law's conduct. No wonder the Church's credibility has suffered so from this arrogant blindness.
The Vatican just doesn't get it. They say they do but, actions speak louder than words.
The Vatican does get it. The get the fact that as long as we continue to live in a caste system where clerical hegemony prevails, they can do whatever they want and no one has to explain anything.
Our Lord is humiliated by the honoring of this derelict bishop — the bishop who refused to protect the little children under his care. He is a scandle of the Church; so is Pope John Paul II who did not publically censure him; so is Pope Benedict who honored that pope who did not do his job.
What a DISGRACE………….I really feel for the poor people hurt by this crumb, Law.
Another example of how the hierarchy have their heads buried in the sand. They just don't get it.
i agree with nancy. it's bad enough he was moved to a cushy job in rome, but now they celebrate a man responsible for so much bad…? what is wrong with our church?
This is disgusting and a major slap in the face to all those who were abused on his watch.
It literally makes me sick to think about this gross slap in the face to the victims, and ultimately to the entire Church.


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Five Minor League Baseball Teams to See This Year
Wednesday, July 16, 2014
Summer isn't complete without a ballpark hot dog and a seventh-inning stretch. Baseball is America's pastime, which means that it's a great reason to travel this summer. It's time to plan a trip to one of these great American cities to cheer a minor league team to victory. These minor league baseball clubs have winning […]
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Small Town Spotlight: Watertown, South Dakota
Friday, July 11, 2014
Explore the beautiful Glacier Lakes region and the area's most popular attractions when you stay at the Country Inn & Suites By Carlson, Watertown in South Dakota. This hotel is ideally located near great attractions, such as Lake Kampeska, Bramble Park Zoo, and the Redlin Art Center. Watertown may be considered a small town, but […]
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Best Places to See Summer Fireworks
Tuesday, July 1, 2014
Summer is simply incomplete without some fun-in-the-sun essentials, like barbecues, boating, and baseball. One of the most time-honored traditions is watching festive fireworks on a warm summer's night. Check out these cities that really know how to light up the evening with spectacular shows set to fun music and amazing views. The Navy Pier in […]
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text: Product Images Alternate Views prev next Share: Email $149.90 - $149.95 Rating 4.69 of 5 (46 Reviews) This Product Qualifies for Free Shipping Please Select a Size Please select your bike Sorry, this item is no longer available. You may be interested in some of the items below. Product Information Now Playing Ogio No Drag Backpack Drop in back panel laptop sleeve Helmet hood Hydration ready Drop in audio pocket Hidden compression strap system Sternum strap No drag streamlined surface Fits most 15" laptops Multiple interior pockets Dimensions: 22"h x 14"w x 7"d 2100 Cu.In/34 L 1.5 Lbs/0.7 Kg Laptop sleeve 13.5"h x 11"w x 1.5"d 420D dobby nylon / molded EVA **Closeouts are limited to stock on hand** Item # 11414 Product Reviews Showing 15 of 46 Reviews Overall Rating: 4.69 of 5 (46 Reviews) Read All Reviews Rate This Product 5 Star 22 4 Star 21 3 Star 3 2 Star 0 1 Star 0 Sort By: Highest Rating Lowest Rating Latest Review What Other Riders Are Saying... 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text: Independent research recently confirmed that 422 Title 1 schools significantly and consistently outperformed their non-Title 1 peers, experiencing dramatically higher gains in test scores than any other schools. The achievements were thanks to many factors, including talented teachers, passionate administrators, and students who had a desire to learn. But there was only one common denominator: On-demand professional development with PD 360.


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Air Tractor sees opportunities to market its AT-802U single-engined turboprop light-attack aircraft in Malaysia and Southeast Asia.
Speaking with Flight International at the Langkawi International Maritime & Aerospace Exhibition on 26 March, vice-president for business development Charles Miller said the type offers a "low-cost solution for reconnaissance and attack".
Miller claims that at an example cost of around $3m before the addition of sensors and weapons, the AT-802U is one-third the price of an attack helicopter.
Kuala Lumpur's lack of a light attack capability was highlighted in early March 2013, when 200 armed intruders from the Philippines infiltrated the country's eastern state of Sabah. The Royal Malaysian Air Force attacked the insurgents with 226kg (500lb) Paveway laser-guided bombs dropped from four BAE Systems Hawk 208s and four Boeing F/A-18D Hornet aircraft, with the targets having been designated by ground units.
Some experts have said that light attack aircraft, such as the Embraer EMB-314 Super Tucano, or attack helicopters would have been more suitable for the Sabah mission.
Miller says the AT-802U is easy to operate from primitive runways and requires little in the way of maintenance facilities.
"The Air Tractor is a good platform if you don't have unlimited funding resources," he says.
Air Tractor
Texas-based Air Tractor has previously sold its armed AT-802U to the United Arab Emirates. Flightglobal's MiliCAS database records its air force as having received 18 of a contracted 23 examples by late 2012.
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Suicide Signs and Prevention
In the aftermath of that tragedy, we're taking a look tonight at suicide prevention. News Source 8's Jenna Callari has a look at some of the signs to watch for if you're worried that someone you know may be in danger of taking their own life.
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This Week In Manga: 9/4-9/10/10
In this week's post: News from Kumoricon, free manga online, manga by subsciption online, manga in libraries, respect for shojo, …Read the Rest
In this week's post: News from Kumoricon, free manga online, manga by subsciption online, manga in libraries, respect for shojo, …Read the Rest
Manga Movable Feast: Kid's Table This month's Manga Movable Feast started this week and doesn't feature just one title. It's …Read the Rest
Open Mouth. Insert Foot Get a bunch of creative people together for a gripe session, and sometimes magic happens! That's …Read the Rest
Hetalia: Axis Powers Does Digital Tokyopop, who has tried to be a leader in digital manga, takes a step to …Read the Rest
Too Much Good Stuff! Deb Aoki of Manga.About.com continues posting her coverage of panels from SDCC. This week she adds …Read the Rest
And the Con Goes On It's been two weeks since Comic-Con, but reports are still coming out with video and …Read the Rest
Twitter This The week started out with a bang, and just kept on going! Seven Seas started it off with …Read the Rest
Anime Expo Con't Anime Expo continued on Saturday and Sunday, but there was only one panel the rest of the …Read the Rest
From Aurora's Ashes Like a phoenix rising from the ashes, the people behind the company have banded together to create …Read the Rest
Movable MANHWA Feast This week started the June edition of the Movable Manga Feast, with one slight change. The manga …Read the Rest
Continuing to Live Up to Their Name It was first announced over the weekend at The Yaoi Review and then …Read the Rest
More Simon and Schuster Sightings The sharp eyes of Michelle Smith of Soliloquy in Blue has spotted some more manga …Read the Rest


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Created: 02/06/2013 10:04 PM KSTP.com
The Minnesota Timberwolves had as special guest Wednesday night for their game against San Antonio. NBA Commissioner David Stern was in town.
It could be the last time he visits Minnesota for a game as the commissioner, since he's retiring from the position after the season.
Stern has had a great relationship with Wolves owner Glen Taylor over the years. Taylor is in the process of selling the franchise, but Stern is confident the team is not on the move.
"He wants it to stay here." Stern said Wednesday. "All discussions about sale revolve around finding the person, or persons, who will commit to keeping it as a first-class franchise in the Twin Cities."


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Creating a Post Consumer Life in Truth or Consequences, New Mexico. Making Our Own Fuel, Power, Food, Medicine, Building Materials and Domestic Goods since 2006.
Kefir Cheese First Try
4 comments:
- miltonics said...
My favorite thing to do with kefir is to mix it with muesli or granola and let it sit out over night.
Kefir also makes a good sourdough bread. You just substitute it for the sourdough culture...
- October 4, 2009 at 8:58 AM
- Starsquid said...
Considering Kefir produces CO2 and lactic acid, could it be the pH of the mixture is getting too low?
Acidic conditions make casein (the protein in milk) congeal but unlike the rennet (enzyme) congealed cheese that most of us are familiar with, it would be more like tofu in consistency- not stretchy at all.
Acid set cheese is called Paneer in India if you want to look it up.
- October 4, 2009 at 9:17 AM
- Uni in Melbourne said...
I tried this recipe too, and found after submerging in cold , water for half an hour, it turned stringy. I also tried booking some cheeses balls (as you do to make halloumi) and the disintegrated. Definitely cold, not heat, for this recipe.
- October 12, 2012 at 7:46 PM
- Uni in Melbourne said...
I tried this recipe, and found that soaking the cheese balls in cold water for half an hr produced the correct stringy texture. Heating them made them disintegrate for me.
- October 12, 2012 at 7:49 PM


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Maybe he can slam dunk over a player and stand there and taunt him after turning the ball over about 30 times while he teams get crushed by 40. That would be about par for the course for an SU player in the NBA.
Only 10 years since our last first round draft pick.....oh what a glorious ten years it has been.
I love all the people who bash Coach Marrone on this site. Seriously folks, all of us fans want SU to do well. However, what makes the bashers think turning this thing around the right way was going to be either quick or easy?
We have outdated facilities and a half empty stadium. The school is located in the declining rust belt and does not benefit from state funds like other schools do.
So realistically, what would make four or five star kids come to SU? It took Schiano a number of years before he got Rutgers on track and that was with a huge windfall of cash from the State of NJ.
So all the bashers, what would you do to fix the program? Please dont say "recruit better". Obviously that is not as easy as everyone thinks it is.
People who bash coach on this site are out of their minds. We are lucky that someone who wants to succeed as badly as Coach does here at SU. He bleeds Orange and is trying to do things the right way. Think of how bad of shape the program was in when he took over. Fans need to support him and realize there is no quick fix here. The point about $ advantages that state schools have over private ones should not be overlooked by any means. It is huge advantage that schools like Rutgers and UConn has over SU. Fans need to be aware of this. Also...if we had hired a guy like Al Golden over Coach....still think he Golden would still be here? People need to realize the that other coaches are NOT drooling for "Syracuse Football HC job".
Bad, bad mistake. Why rush into draft to be a late first round pick to sit on the bench? The other posters are probably correct about team play next year. However, the math does not work in Deion's favor. The rush to sign with an agent is????? See Jonny Flynn. Good luck to you Dion but the "quick buck" grab is short sighted and a bad business decision.
Great post. You are correct. I was watching the game thinking the same thing. While I am obviously an SU fan, Triche should been awarded two foul shots at the end of the first half. Bad call against us. He was hammered on the out of bounds play, that did go off of him. However, that was clearly a foul. And the lane violation was the right call. Reggie Miller was awful and was clearly on the bandwagon. How does he even have a job - he was terrible in his analysis and his voice is so annoying. At least Len Elmore was trying to bring Harlan and Miller back to planet earth.
This team has not played well is a while. Even with Fab, I was very, very worried we were going to be bounced by K-State.
Without guys willing to rebound there was no way we were going to win the tournament.
Look on the bright side, at least this is an excuse and it wont hurt as badly when make another early exit.
Please no videos. I will never watch them. Most of us still like and know how to read. Please write your thoughts...videos come across as "I am too lazy to write".
6-6 and beating Rutgers would be a really good first year. I mean Robinson was such a terrible coach....+3 on that factor from last year should not be too much of a reach...should it?
I don't believe the University can do this because of NCAA rules wont allow them do it.
I asked asked about having something set up that would allow the University to coordinate it for those who wanted to help out but cant go.
That said, I just bought seasons tickets myself and likely can only go to one game this year. I will be donating the rest to BBBS. I hope more folks like myself who can't go do the same thing.
Thanks to those who started the idea!
Rightfully so until we prove otherwise.
It is amazing how much the program slipped under the "Club" Robinson era. The real question is how and why did it take Dr. Gross so long to realize his players were not even tested for strength and conditioning. Seriously. Really? How could that fall through the cracks?
Totally agreed. Seriously, can we take down this completely unimportant news story related to SU football? Seriously, no one cares about this garbage or noise. How does this qualify as news?
Please take this post down. Can we have something related to this year's team to read about? Even a story about how the color of the goal posts has faded (on the practice field) because of the sun is more relevant than this.
Why is this even worth reporting on SU football? How about reporting some of the good deeds the kids do like community service, etc. I hardly find this relevant and get annoyed every time I come to the site and see it posted. Great reporting.
Totally agree. This is a no brainer. Syracuse football has become the laughing stock of D1 football...we are now the Rugters...of the 1980s - 1990s..that really hurts to say that but it is so true.
Not only would it give us the best QB since Donovan talent wise since Donovan...it would also give SU "constant" national publicity and perhaps open the minds of recruits who would not consider SU since we have become this bad.
I cant see how recruiting a hard-working, very talented, smart local kid can been seen as anything but positive. People should stop holding the decision of a 17-year old kid against him and should root that he signs on with SU.
BTW, when was it bad to look out for one's best interests and explore all options? Also, maybe the rest of the team could learn something about working hard, facing adversity and not quitting as soon as things don't go their way...a trait that is coming across pretty loud and clear by all the guys quitting the team lately.
Now this is good, good stuff.
Great job on the tidbits. I really like the insight.
44 never should have been retired and it was a major mistake by Dr. Gross.
44 is a huge part of Syracuse University and its tradition. Giving the number to select recruits will only keep the lore of 44 alive and provides a connection with the student body and Syracuse community.
In my opinion, by having 44 sitting up in the Carrier Dome rafters only serves to distance current athletes and students from the history of Syracuse University. Yes, I concede that the players who "could" wear 44 may not live up to the football excellence that Ernie Davis, Jim Brown, and Floyd Little established. But do we really expect them to? Rather it is an ideal...something that those players strive to achieve and accept the responsibility of representing that number. By keeping 44 active, the tradition, lore, and level of excellence associated with 44 is more likely to stay relevant to all Syracuse University stakeholders.
I believe the current coaching staff should have the ability to give the number to a prospective "special student athletes" when deemed appropriate. It does not have to nor should it be handed out every four years. Rather, it should remain active to remind players and the student body of the values and ideals that 44 represents on and off the field. As Coach Marrone has stated, he is not only looking for great football players but also he wants great football players with outstanding character. He also wants his fellow coaches to have Syracuse University ties. I think having 44 at his disposal will only serve to help him acheive his vision.
To be honest, 44 should never have been retired by Dr. Gross - a person who said it took him "some time" to understand the area (Syracuse and the Northeast) after arriving from USC.
This mistake can and should be undone. It actually makes a lot of sense with the new regime and their efforts to energize the alumni, community, and student body around the football program.
Restore 44 is an easy decision for me.
John Tomlinson, Class of 1992
Coach Robinson is only partly to blame. Dr. Gross deserves major heat as well for bringing back a lame duck coach and not getting rid of him earlier in the season (Akron or Penn State).
Let's hope so. Regardless of needs, you would think we have more than 6 to 10 players rolling off....if we can make it up next year...then more power to Coach. I have total confidence in him right now.
All good points. Not much expected.
This is not a class at this point but merely a study group. There is a lot of time left but how this class gets to 12...would be quite an accomplishment.