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A biosensor includes a substrate with a layer of receptive material disposed thereon overlying a layer containing a photo-reactive agent. The receptive material is specific for an analyte of interest. A pattern of active and inactive areas of the receptive material are defined in the receptive material layer by a masking process wherein the photo-reactive agent is activated in the exposed regions of the mask.
1. A biosensor, comprising:
a substrate member defining a surface;
a photo-reactive agent overlying the surface of said substrate member;
a receptive material layer applied directly over said photo-reactive agent and generally uniformly covering said surface of said substrate member, said receptive material being specific for an analyte of interest;
wherein a pattern of active and inactive areas is defined by said receptive material layer, said active and inactive areas formed by a masking process wherein a mask is placed over said substrate member prior to irradiating said substrate member with an energy source sufficient for activating said photo-reactive agent such that areas exposed by said mask define said pattern of inactive areas of said receptive material and said areas shielded by said mask define said pattern of active areas of receptive material; and
wherein said biosensor is configured to detect the presence of an analyte of interest when exposed to a medium containing said analyte of interest by binding the analyte to said receptive material in said active areas and subsequently facilitate diffraction of transmitted light or reflected light in a diffraction pattern corresponding to said active areas.
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14. A biosensor, comprising:
a substrate member defining a surface;
a photo-reactive agent overlying the surface of the substrate member;
a receptive material layer molecularly attached to the photo-reactive agent, the receptive material being specific for an analyte of interest; wherein the receptive material layer defines active areas and inactive areas; wherein the active areas are configured to bind to an analyte of interest, and wherein the inactive areas comprise activated photo-reactive agent and denatured receptive material such that the denatured receptive material does not bind to the analyte of interest;
wherein the biosensor is configured to detect the presence of an analyte of interest in a medium through diffraction of transmitted light or reflected light.
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The present application is a divisional application of U.S. patent application Ser. No. 10/139,013 having a filing date of May 3, 2002 and relates generally to the field of detecting analytes in a medium, and more particularly to a process for preparing analyte-specific diagnostic sensors to indicate the presence of the analyte in a medium in, for example, a diffraction/holography format.
There are many systems and devices available for detecting a wide variety of analytes in various media. Many of the prior systems and devices are, however, relatively expensive and require a trained technician to perform the test. A need has been recognized in the art for biosensor systems that are easy and inexpensive to manufacture, and capable of reliable and sensitive detection of analytes. Reference is made, for example, to U.S. Pat. Nos. 5,922,550; 6,060,256; and 6,221,579 B1.
Various advances have been made in the industry for producing biosensors. For example, U.S. Pat. No. 5,512,131 to Kumar, et al., describes a device that includes a polymer substrate having a metal coating. An analyte specific receptor layer is stamped onto the coated substrate. A diffraction pattern is generated when an analyte binds to the device. A visualization device, such as a spectrometer, is then used to determine the presence of the diffraction pattern. A drawback to this type of device is, however, the fact that the diffraction pattern is not discernible by the naked eye and, thus, a complex visualization device is needed to view the diffraction pattern. Also, the device is generally not able to detect smaller analytes that do not produce a noticeable diffraction pattern.
U.S. Pat. No. 5,482,830 to Bogart, et al., describes a device that includes a substrate which has an optically active surface exhibiting a first color in response to light impinging thereon. This first color is defined as a spectral distribution of the emanating light. The substrate also exhibits a second color which is different from the first color. The second color is exhibited in response to the same light when the analyte is present on the surface. The change from one color to another can be measured either by use of an instrument, or by the naked eye. A drawback with the device is, however, the relatively high cost of the device and problems associated with controlling the various layers that are placed on the wafer substrate.
Contact printing techniques have been explored for producing biosensors having a self-assembling monolayer. U.S. Pat. No. 5,922,550 describes a biosensor having a metalized film upon which is printed (contact printed) a specific predetermined pattern of an analyte-specific receptor. The receptor materials are bound to the self-assembling monolayer and are specific for a particular analyte or class of analytes. Attachment of a target analyte that is capable of scattering light to select areas of the metalized plastic film upon which the receptor is printed causes diffraction of transmitted and/or reflected light. A diffraction image is produced that can be easily seen with the eye or, optionally, with a sensing device. U.S. Pat. No. 6,060,256 describes a similar device having a metalized film upon which is printed a specific predetermined pattern of analyte-specific receptor. The '256 patent is not limited to self-assembling monolayers, but teaches that any receptor which can be chemically coupled to a surface can be used. The invention of the '256 patent uses methods of contact printing of patterned monolayers utilizing derivatives of binders for microorganisms. One example of such a derivative is a thiol. The desired binding agent can be thiolated antibodies or antibody fragments, proteins, nucleic acids, sugars, carbohydrates, or any other functionality capable of binding an analyte. The derivatives are chemically bonded to metal surfaces such as metalized polymer films, for example via a thiol.
A potential issue of the contact printing techniques described above for producing diffraction-based biosensors is the possibility of contamination from the print surface (i.e., stamp) during the printing process. Also, there is the possibility of uneven application or inking of the substances due to pressure and contact variations inherent in the process, as well as surface energy variations.
The present invention relates to a biosensor system that is easy and inexpensive to manufacture, is capable of reliable and sensitive detection of analytes, and avoids possible drawbacks of conventional microcontact printing techniques.
Objects and advantages of the invention will be set forth in part in the following description, or may be obvious from the description, or may be learned through practice of the invention.
The present invention provides a relatively inexpensive yet sensitive biosensor device, a method for producing such biosensor devices, and a method for detecting analytes of interest present in a medium.
The biosensor includes a substrate containing a photo-reactive agent. The agent may be applied generally uniformly as a layer over an entire surface of the substrate member. Alternatively, the substrate may contain the agent as an integral component thereof. The photo-reactive agent may be, for example, a photo-acid or photo-oxidant. The agent may be in solution in a "transparent" polymer, for example PVC, polystyrene, and the like, and a thin film of the solution may be applied on the substrate member by, for example, a spin-coating process. In alternate embodiments, the agent may be embedded or polymerized in the substrate. A special plasticizer may incorporate the agent. The substrate may be formed of a material that produces an acid, base, or oxidant, upon exposure to a particular stimulus (e.g. light).
The substrate may be any one of a wide variety of suitable materials, including plastics, metal coated plastics and glass, functionalized plastics and glass, silicon wafers, foils, glass, etc.
The photo-reactive agent may be applied as a layer in a light-protected environment by any number of known techniques, including dipping, spraying, rolling, spin coating and any other technique wherein the layer can be applied generally uniformly over the entire test surface of the substrate. The invention also includes contact printing methods of applying the photo-reactive agent layer.
A layer containing a receptive material (e.g., biomolecules) is then applied over the photo-reactive agent layer also in a light-protected environment. The receptive material layer may be applied by any number of known techniques, including dipping, spraying, rolling, spin coating and any other technique wherein the layer can be applied generally uniformly over the entire test surface of the substrate. The invention also includes contact printing methods of applying the receptive material layer, as long as such methods are conducted in a manner to prevent inconsistent inking and contamination from contact during the coating process.
The receptive material layer is then defined into a pattern of active and inactive areas of receptive material by placing a mask over the substrate and subsequently irradiating the substrate with an energy source sufficient to activate the photo-reactive agent in the exposed or unshielded areas of the mask. Activation of the photo-reactive agent results in denaturing of the overlying biomolecules in the exposed areas. The receptive material is thus "deactivated" to the extent that it can no longer bind with conjugate ligands, including the analyte of interest.
The mask may include any desired pattern of protected or shielded areas and exposed areas (e.g., blank, transparent, or translucent areas, as well as holes or openings in the mask structure). The exposed areas of the mask define a pattern of inactive areas of the receptive material and the shielded areas of the mask define a pattern of active receptive material areas. The mask thus serves to shield an area of the receptive material layer and to expose at least one adjacent area to the irradiating energy source for activating the underlying photo-reactive agent.
It should be appreciated that the invention is not limited to any particular pattern defined by the mask. Virtually any number and combination of exposed shapes or openings are possible. In one particular embodiment, the pattern is defined by about 10 micron diameter pixels at a spacing of about 5 microns over the test surface of the substrate.
The photo-reactive agent and receptive material layers are irradiated with an energy source selected particularly for activating the specific type of photo-reactive agent. The invention is not limited to any particular energy source. For example, the energy source may be a light source, e.g., an ultraviolet (UV) light source, an electron beam, a radiation source, etc.
Upon subsequent exposure of the biosensor to a medium containing an analyte of interest, the analyte binds to the receptive material in the active areas. The biosensor will then diffract transmitted light in a diffraction pattern corresponding to the active areas. The diffraction pattern may be visible to the naked eye or, optionally, viewed with a sensing device.
In the case where an analyte does not scatter visible light because the analyte is too small or does not have an appreciable refractive index difference compared to the surrounding medium, a diffraction-enhancing element, such as polymer microparticles, may be used. These micorparticles are coated with a binder or receptive material that also specifically binds to the analyte. Upon subsequent coupling of the analyte to both the patterned biomolecules in the receptive material layer as well as the microparticles, a diffraction image is produced which can be easily seen with the eye or, optionally, with a sensing device.
By "diffraction" it is meant the phenomenon, observed when waves are obstructed by obstacles, of the disturbance spreading beyond the limits of the geometrical shadow of the object. The effect is marked when the size of the object is of the same order as the wavelength of the waves. In the present invention, the obstacles are analytes (with or without or attached microparticles) and the waves are light waves.
In another embodiment of the present invention, nutrients for a specific class of microorganisms can be incorporated into the receptive material layer. In this way, very low concentrations of microorganisms can be detected by first contacting the biosensor of the present invention with the nutrients incorporated therein and then incubating the biosensor under conditions appropriate for the growth of the bound microorganism. The microorganism is allowed to grow until there are enough organisms to form a diffraction pattern.
The present invention provides a low-cost, disposable biosensor which can be mass produced. The biosensors of the present invention can be produced as a single test for detecting an analyte or it can be formatted as a multiple test device. The uses for the biosensors of the present invention include, but are not limited to, detection of chemical or biological contamination in garments, such as diapers, the detection of contamination by microorganisms in prepacked foods such as meats, fruit juices or other beverages, and the use of the biosensors of the present invention in health diagnostic applications such as diagnostic kits for the detection of hormones, proteins, antigens, nucleic acids, microorganisms, and blood constituents. It should be appreciated that the present invention is not limited to any particular use or application.
These and other features and advantages of the present invention will become apparent after a review of the following detailed description of the disclosed embodiments.
The invention will now be described in detail with reference to particular embodiments thereof. The embodiments are provided by way of explanation of the invention, and not meant as a limitation of the invention. For example, features described or illustrated as part of one embodiment may be used with another embodiment to yield still a further embodiment. It is intended that the present invention include these and other modifications and variations as come within the scope and spirit of the invention.
The present invention features improved biosensing devices, and methods for using such biosensing devices, for detecting and quantifying the presence or amount of an analyte of interest within a medium. The analytes that can be detected by the present invention include, but are not limited to, microorganisms such as bacteria, yeasts, fungi and viruses. The biosensing devices according to the invention are relatively inexpensive and have advantages over conventional micro-printed biosensors.
The present invention comprises, in broad terms, a process of defining an active pattern of analyte-specific receptive material on a substrate surface by photo-masking the substrate. A photo-reactive agent is incorporated with the substrate member, for example by spin-coating a solution containing the agent onto the substrate member. Alternatively, the agent may be embedded or polymerized in the substrate. In another embodiment, the substrate may be formed in part or entirely of a photo-reactive substance. For example, the substrate may be PVC (polyvinyl chloride) which will produce HCL (hydrochloric acid) upon exposure to UV light. Polyamine will produce a base upon exposure to UV light. A generally uniform coating of the receptive material is then applied to the substrate surface over the photo-reactive agent. A mask is placed over the substrate, and the mask and substrate combination is irradiated with an energy source specifically selected to activate the photo-reactive agent. In its basic form, the "mask" serves to shield at least one area or section of the substrate member from the irradiating energy source and to expose at least one adjacent section to the energy source. For example, the mask may be a generally transparent or translucent blank (e.g., a strip of material) having any pattern of shielded regions printed or otherwise defined thereon. The exposed unshielded regions of the mask correspond to the exposed areas of the substrate member. Alternatively, the mask may simply be a single object placed upon the substrate. The area under the object would be shielded and thus define an active area of the receptive material, and the area around the object would be exposed to the energy source and thus define an area of inactive receptive material. Alternatively, the object may have any pattern of openings defined therethrough corresponding to the exposed areas.
As mentioned, the energy source is selected so that the exposed photo-reactive agent is activated and thus denatures the overlying biomolecules, thereby rendering the receptive material inactive in the exposed regions. Specifically, the activated photo-reactive agent may be any one of a number of substances, such asdiaryliodonium salts, triarylsulfonium salts, diazosulfonates, bromobisphenol A, certain Cl containing polymers, acid or base producing polymers, dialkylphenacylsulfonium salts, etc., or photo-oxidants such as ZnO, TiO2, and certain copper and ruthenium complexes. In one particular embodiment, the agent is a photo-acid, such as Triphenylsulfonium triflate, (4-iodophenyl) diphenylsulfonium triflate, Diphenyliodonium p-toluenesulfonate, Bis(4-t-butylphenyl) iodonium p-toluenesulfonate, Bis(4-t-butylphenyl) iodonium triflate, and Tris(4-t-butylphenyl) sulfonium triflate. In another embodiment, the agent may be a photo-oxidant, such as ZnO; TiO2; Cu[2,9-diphenyl-1-10-phenanthroline]2 + in the presence of nitrobenzylbromide; and Ru[2,2′-bipyridine]3 2+ in the presence of Co[(NH3)5Cl]2+. The photo-reactive agent in the regions shielded by the mask remains "unactivated." Thus, upon removal of the mask, a pattern of active and inactive receptive material areas are defined on the substrate member. It should be understood that "pattern" includes as few as one active area and one inactive area.
Upon subsequent exposure of the biosensor to a medium containing the analyte of interest, such analyte will bind to the biomolecules in the active areas. The analyte results in diffraction of transmitted and/or reflected light in a visible diffraction pattern corresponding to the active areas. As discussed in greater detail below, an enhancer may be used for enhancing diffraction from extremely small analytes.
The analytes that are contemplated as being detected using the present invention include, but are not limited to, bacteria; yeasts; fungi; viruses; rheumatoid factor; antibodies, including, but not limited to IgG, IgM, IgA, IgD, and IgE antibodies; carcinoembryonic antigen; streptococcus Group A antigen; viral antigens; antigens associated with autoimmune disease, PSA (prostate specific antigen) and CRP (C-reactive protein) antigens; allergens; tumor antigens; streptococcus Group B antigen; HIV I or HIV II antigen; or host response (antibodies) to these and other viruses; antigens specific to RSV or host response (antibodies) to the virus; antigen; enzyme; hormone; polysaccharide; protein; lipid; carbohydrate; drug or nucleic acid; Salmonella species; Candida species, including, but not limited to Candida albicans and Candida tropicalis; Neisseria meningitides groups A, B, C, Y and W sub 135, Streptococcus pneumoniae; E. coli; Haemophilus influenza type A/B; an antigen derived from microorganisms; a hapten; a drug of abuse; a therapeutic drug; an environmental agent; and antigens specific to Hepatitis. In broad terms, the "analyte of interest" may be thought of as any agent whose presence or absence from a biological sample is indicative of a particular health state or condition.
It is also contemplated that nutrients for a specific class of microorganism can be incorporated into the receptive material layer. In this way, very low concentrations of microorganisms can be detected by exposing the biosensor of the present invention with the nutrients incorporated therein to the suspect medium and then incubating the biosensor under conditions appropriate for the growth of the bound microorganism. The microorganisms are allowed to grow until there are enough organisms to form a diffraction pattern. Of course, in some cases, the microorganism is present or can multiply enough to form a diffraction pattern without the presence of a nutrient in the active receptive material areas.
The receptive material is characterized by an ability to specifically bind the analyte or analytes of interest. The variety of materials that can be used as receptive material is limited only by the types of material which will combine selectively (with respect to any chosen sample) with a secondary partner. Subclasses of materials which fall in the overall class of receptive materials include toxins, antibodies, antibody fragments, antigens, hormone receptors, parasites, cells, haptens, metabolites, allergens, nucleic acids, nuclear materials, autoantibodies, blood proteins, cellular debris, enzymes, tissue proteins, enzyme substrates, coenzymes, neuron transmitters, viruses, viral particles, microorganisms, proteins, polysaccharides, chelators, drugs, aptamers, peptides, and any other member of a specific binding pair. This list only incorporates some of the many different materials that can be coated onto the substrate surface to produce a thin film assay system. Whatever the selected analyte of interest is, the receptive material is designed to bind specifically with the analyte of interest.
The matrix or medium containing the analyte of interest may be a liquid, a solid, or a gas, and can include a bodily fluid such as mucous, saliva, urine, fecal material, tissue, marrow, cerebral spinal fluid, serum, plasma, whole blood, sputum, buffered solutions, extracted solutions, semen, vaginal secretions, pericardial, gastric, peritoneal, pleural, or other washes and the like. The analyte of interest may be an antigen, an antibody, an enzyme, a DNA fragment, an intact gene, a RNA fragment, a small molecule, a metal, a toxin, an environmental agent, a nucleic acid, a cytoplasm component, pili or flagella component, protein, polysaccharide, drug, or any other material. For example, receptive material for bacteria may specifically bind a surface membrane component, protein or lipid, a polysaccharide, a nucleic acid, or an enzyme. The analyte which is specific to the bacteria may be a polysaccharide, an enzyme, a nucleic acid, a membrane component, or an antibody produced by the host in response to the bacteria. The presence or absence of the analyte may indicate an infectious disease (bacterial or viral), cancer or other metabolic disorder or condition. The presence or absence of the analyte may be an indication of food poisoning or other toxic exposure. The analyte may indicate drug abuse or may monitor levels of therapeutic agents.
One of the most commonly encountered assay protocols for which this technology can be utilized is an immunoassay. However, the general considerations apply to nucleic acid probes, enzyme/substrate, and other ligand/receptor assay formats. For immunoassays, an antibody may serve as the receptive material or it may be the analyte of interest. The receptive material, for example an antibody or an antigen, must form a stable, relatively dense, reactive layer on the substrate surface of the test device. If an antigen is to be detected and an antibody is the receptive material, the antibody must be specific to the antigen of interest; and the antibody (receptive material) must bind the antigen (analyte) with sufficient avidity that the antigen is retained at the test surface. In some cases, the analyte may not simply bind the receptive material, but may cause a detectable modification of the receptive material to occur. This interaction could cause an increase in mass at the test surface or a decrease in the amount of receptive material on the test surface. An example of the latter is the interaction of a degradative enzyme or material with a specific, immobilized substrate. In this case, one would see a diffraction pattern before interaction with the analyte of interest, but the diffraction pattern would disappear if the analyte were present. The specific mechanism through which binding, hybridization, or interaction of the analyte with the receptive material occurs is not important to this invention, but may impact the reaction conditions used in the final assay protocol.
In addition to producing a simple diffraction image, patterns of analytes can be such as to allow for the development of a holographic sensing image and/or a change in visible color. Thus, the appearance of a hologram or a change in an existing hologram will indicate a positive response. The pattern made by the diffraction of the transmitted light can be any shape including, but not limited to, the transformation of a pattern from one pattern to another upon binding of the analyte to the receptive material. In particularly preferred embodiments, the diffraction pattern becomes discernible in less than one hour after contact of the analyte with the biosensing device of the present invention.
The diffraction grating which produces the diffraction of light upon interaction with the analyte must have a minimum periodicity of about ˝ the wavelength and a refractive index different from that of the surrounding medium. Very small analytes, such as viruses or molecules, can be detected indirectly by using a larger, "diffraction-enhancing element," such as a micro-particle, that is specific for the small analyte. One embodiment in which the small analyte can be detected comprises coating the enhancing particle, such as a latex bead or polystyrene bead, with a receptive material, such as an antibody, that specifically binds to the analyte of interest. Particles that can be used in the present invention include, but are not limited to, glass, cellulose, synthetic polymers or plastics, latex, polystyrene, polycarbonate, proteins, bacterial or fungal cells, silica, cellulose acetate, carbon, and the like. The particles are desirably spherical in shape, but the structural and spatial configuration of the particles is not critical to the present invention. For instance, the particles could be slivers, ellipsoids, cubes, random shape and the like. A desirable particle size ranges from a diameter of approximately 0.1 micron to 50 microns, desirably between approximately 0.1 micron and 2.0 microns. The composition of the particle is not critical to the present invention.
Desirably, the receptive material layer on the substrate will specifically bind to an epitope on the analyte that is different from the epitope used in the binding to the enhancing particle. Thus, for detecting a small analyte, such as viral particles, in a medium, the medium is first exposed to the latex particles having the virus-specific receptive material thereon. The small analytes of interest in the medium will bind to the latex particles. Then, the latex particles are optionally washed and exposed to the biosensor film with the pattern of active receptive material areas containing the virus-specific antibodies. The antibodies then bind to the viral particles on the latex bead thereby immobilizing the latex beads in the same pattern as the active areas on the film. Because the bound latex beads will cause diffraction of the visible light, a diffraction pattern is formed, indicating the presence of the viral particle in the liquid. Other combinations using diffraction enhancing particles are described, for example, in U.S. Pat. No. 6,221,579 incorporated herein for all purposes.
Any one of a wide variety of materials may serve as the substrate to which the receptive material is applied. Such materials are well known to those skilled in the art. For example, the substrate may be formed of any one of a number of suitable plastics, metal coated plastics and glass, functionalized plastics and glass, silicon wafers, foils, glass, acid or base producing polymers, etc. It should be appreciated that, if the photo-reactive agent is a photo-acid, the substrate would not be metal or metal coated. Thermoplastic films have been shown to be quite suitable. Such films include, but are not limited to, polymers such as: polyethylene-terephthalate (MYLAR®), acrylonitrile-butadiene-styrene, acrylonitrile-methyl acrylate copolymer, cellophane, cellulosic polymers such as ethyl cellulose, cellulose acetate, cellulose acetate butyrate, cellulose propionate, cellulose triacetate, cellulose triacetate, polyethylene, polyethylene-vinyl acetate copolymers, ionomers (ethylene polymers) polyethylene-nylon copolymers, polypropylene, methyl pentene polymers, polyvinyl fluoride, and aromatic polysulfones. Preferably, the plastic film has an optical transparency of greater than 80 percent. Other suitable thermoplastics and suppliers may be found, for example, in reference works such as the Modern Plastics Encyclopedia (McGraw-Hill Publishing Co., New York 1923-1996).
In one embodiment of the invention wherein the photo-reactive agent is a photo-oxidant, the thermoplastic film may have a metal coating. The film with metal coating thereon may have an optical transparency of between approximately 5 percent and 95 percent. A more desired optical transparency for the thermoplastic film used in the present invention is between approximately 20 percent and 80 percent. In a desired embodiment of the present invention, the thermoplastic film has at least an approximately 80 percent optical transparency, and the thickness of the metal coating is such as to maintain an optical transparency greater than about 20 percent, so that diffraction patterns can be produced by either reflected or transmitted light. This corresponds to a metal coating thickness of about 20 nanometers. However, in other embodiments of the invention, the metal thickness may be between approximately 1 nanometer and 1000 nanometers.
The preferred metal for deposition on the film is gold. However, silver, aluminum, chromium, copper, iron, zirconium, platinum, titanium, and nickel, as well as oxides of these metals, may be used. Chromium oxide can be used to make metalized layers.
The receptive material may be applied to the substrate over the photo-reactive layer by any conventional method. The material is applied so that it generally uniformly covers an entire (i.e. upper) surface of the substrate. Non-contact methods for applying the receptive material may be desired so as to eliminate the possibility of contamination by contact printing device. Such non-contact methods include, but are not limited to, dipping, spraying, rolling, spin coating, and any other technique wherein the receptive material layer can be applied generally uniformly over the entire test surface of the substrate. Simple physisorption can occur on many materials, such as polystyrene, glass, nylon, or other materials well known to those skilled in the art. One particular embodiment of immobilizing the analyte-specific receptive material layer involves molecular attachment, such as that possible between thiol or disulfide-containing compounds and gold. Typically, a gold coating of about 5 to about 2000 nanometers thick is supported on a silicon wafer, glass, or polymer film (such as a MYLAR® film). The analyte-specific receptor attaches to the gold surface during immersion or spraying of a solution of the receptive material.
Although not preferred, the invention also includes contact printing methods of applying the receptive material. The technique selected should minimize the amount of receptive material required for coating a large number of test surfaces and maintain the stability/functionality of the receptive material during application. The technique should also apply or adhere the receptive material to the substrate in a uniform and reproducible fashion.
It is also contemplated that the receptive material layer may be formed on the substrate as a self-assembling monolayers of alkanethiolates, carboxylic acids, hydroxamic acids, and phosphonic acids on metalized thermoplastic films. The self-assembling monolayers have receptive material bound thereto. Reference is made to U.S. Pat. No. 5,922,550 for a more detailed description of such self-assembling monolayers and methods for producing the monolayers. The '550 patent is incorporated herein in its entirety for all purposes.
The mask may be formed of any suitable material that shields the underlying portion of the substrate from the irradiating energy source. A material that has proven useful for defining patterns of active and inactive receptive material regions on a gold-plated MYLAR® film coated with an antibody solution is a transparent or translucent polymer film (such as MYLAR®) having a pattern of blocked or protected regions printed thereon. This type of mask is useful for light sources (irradiating energy source) with a wavelength equal to or greater than about 330 nanometers. For light sources having a wavelength below about 330 nanometers, a quartz or fused silica mask having chrome plated blocked regions defined thereon may be used. It may be desired to select a hole pattern and size so as to maximize the visible diffraction contrast between the active and inactive regions. It has been found suitable if the active regions are defined as generally circular with a diameter of about 10 microns and spaced from each other by about 5 microns.
Any suitable energy source may be selected for irradiating the mask and substrate combination. An energy source is selected particularly for activating the specific type of photo-reactive agent. The energy source may be, for example, a light source, e.g., an ultraviolet (UV) light source, an electron beam, a radiation source, etc. In one particular embodiment, the photo-reactive agent is a photo-acid or a photo-oxidant, such as Triphenyulsulfonium triflate or TiO2, respectively, and the activating energy source is a UV light source. The sensor is exposed to the light source for a period of time sufficient for the photo-reactive agent to be activated and thus render the receptive material in the exposed areas inactive. Depending on the particular type of photo-reactive agent, light of a proper wavelength generates either an acid, base, or an oxidant in the illuminated regions. These relatively large localized concentrations of an acid, base, or oxidant serve to denature the proteins in the receptive material. The "denaturing" occurs either through acid-catalyzed hydrolysis of the antibody, pH-induced conformational changes, or via oxidation/radical formation of/on the antibody, or a combination of such factors. In the case of chlorinated polymers, irradiation generates HCl, and the localized increase in acid concentration serves to denature the antibody. It should be appreciated that the invention is not limited to any particular type of light or activating energy source or exposure times. The type of light (e.g., wavelength) and exposure times may vary depending on the particular type of photo-reactive agent. Other suitable energy sources may include tuned lasers, electron beams, various types of radiation beams including gamma and X-ray sources, various intensities and wavelengths of light including light beams of sufficient magnitude at the microwave and below wavelengths, etc. Care should be taken that the energy source does not damage (e.g., melt) the underlying substrate or mask.
The biosensors according to the invention have a wide range of uses in any number of fields. The uses for the biosensors of the present invention include, but are not limited to, detection of chemical or biological contamination in garments, such as diapers, generally the detection of contamination by microorganisms in prepacked foods such as meats, fruit juices or other beverages, and the use of the biosensors of the present invention in health diagnostic applications such as diagnostic kits for the detection of proteins, hormones, antigens, nucleic acids, DNA, microorganisms, and blood constituents. The present invention can also be used on contact lenses, eyeglasses, window panes, pharmaceutical vials, solvent containers, water bottles, band-aids, wipes, and the like to detect contamination. In one embodiment, the present invention is contemplated in a dipstick form in which the patterned substrate is mounted at the end of the dipstick. In use the dipstick is dipped into the liquid in which the suspected analyte may be present and allowed to remain for several minutes. The dipstick is then removed and then, either a light is projected through the substrate or the substrate is observed with a light reflected from the substrate. If a diffraction pattern is observed, then the analyte is present in the liquid.
In another embodiment of the present invention, a multiple analyte test is constructed on the same support. A strip may be provided with several patterned substrate sections. Each section has a different receptive material that is different for different analytes. It can be seen that the present invention can be formatted in any array with a variety of patterned substrates thereby allowing the user of the biosensor device of the present invention to detect the presence of multiple analytes in a medium using a single test.
In yet another embodiment of the present invention, the biosensor can be attached to an adhesively backed sticker or decal which can then be placed on a hard surface or container wall. The biosensor can be placed on the inside surface of a container such as a food package or a glass vial. The biosensor can then be visualized to determine whether there is microbial contamination.
It should be understood that the invention includes various other embodiments, modifications, and equivalents to the examples described herein which, after reading the description of the invention, may become apparent to those skilled in the art without departing from the scope and spirit of the present invention.
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24 May 2013 13:06 GMT | Europe, US & Canada, Afghanistan, United Kingdom, United States
Police in London have arrested a man and a woman in connection with the murder of a British soldier near a military barrack.Detectives said on Thursday they had arrested the unidentified suspects, both aged 29, over the murder of Drummer Lee Rigby, 25, who was hacked to death in Woolwich on Wednesday."This is a large, complex and fast-moving investigation which continues to develop," London police said in a statement. "Many lines of inquiry are being followed by detectives and the investigation is progressing well."
Detectives said they were searching six houses; three in Greenwich in south London, one in Romford, east London, one in north London, and a property in Lincoln in central England.Meanwhile, Rigby's two main suspected attackers were in separate hospitals being treated for gun shot wounds. They were fired at by officers at the scene of the killing.
A new video has emerged of the two suspects, who appear to run at the police as they arrived at the scene of the crime.
They had been known to MI5 security services before the attack, reports said.
One man is believed to be 28-year-old Londoner Michael Adebolajo, who is of Nigerian descent, and is said to have converted to Islam 10 years ago.
Rigby served in Afghanistan in 2009, Britain's Ministry of Defence said.
Extra police
London deployed more than 1,200 extra police officers on the capital's streets amid fears of a backlash on British Muslims.
This action was a betrayal of Islam and the Muslim communities that give so much to our country ... we will not rest until we know every detail.
David Cameron, UK prime minister
The attackers had made references to Islam in amateur footage broadcast on television.
Footage broadcast by Britain's ITV news channel showed a man, with hands soaked in blood and holding a meat cleaver and a knife, claiming that he had, motivated by Britain's foreign policy, killed a soldier.
Witnesses said he requested to be filmed by a passerby and shouted "Allahu Akbar" during the killing.
In the amateur video, he said: "I apologise that women had to witness this today but in our lands, our women have to see the same ... you people will never be safe. Remove your government, they don't care about you."
The second attacker is believed to be 22 years old.
'Standing together'
Later on Thursday, President Barack Obama offered his support to Britain saying the US "stands resolute with the United Kingdom, our ally and friend, against violent extremism and terror," in a statement.
"I look forward to my trip to the United Kingdom to participate in the June G-8 Summit, hosted by Prime Minister Cameron, which will include discussions on the important global security challenges our countries face together."
After a second meeting of the government's emergency COBRA security council on Thursday, Cameron said the country would "defeat violent extremism by standing together".
"This action was a betrayal of Islam and the Muslim communities that give so much to our country ... we will not rest until we know every detail."
Cameron also praised the actions of Ingrid Loyau-Kennett, a cub scout leader who confronted the attackers immediately after the violence and tried to talk them down.
"They told her they wanted to start a war in London and she replied, 'you are going to lose, it is you against many," Cameron said. "She speaks for all of us."
Al Jazeera's Laurence Lee, reporting from Woolwich, said it was essential that political and religious leaders restore calm in the face of violence from the UK's far-right.
"The English Defence League wanted to use this to their advantage. They came out last night chanting 'no surrender to Muslims,' and clashed with riot police," our correspondent said.
Police in the county of Kent, south of London, said they had charged a man with "religiously aggravated criminal damage and burglary" to a mosque.
The Muslim Council of Britain condemned the Woolwich attack, saying: "This is a truly barbaric act that has no basis in Islam and we condemn this unreservedly."
Source: Al Jazeera and agencies
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Media, #FreeAJStaff, Egypt, Middle East
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Human Rights, Israel, Palestine
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Recipe by mimi52
"A mint julep with a twist. If you don't have enough room in your glass for 2.5 ounces of sweet tea, just top it off."
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2 tablespoons
simple syrup
fresh mint leaves
1/2 cup
crushed ice, or as needed
2 fluid ounces
peach-flavored bourbon liqueur (such as Southern Comfort ®)
1 1/2 fluid ounces
peach schnapps
2 fluid ounces
club soda
2 1/2 fluid ounces
sweet tea, or as needed
I feel like sitting on porch swing talking in a southern accent (badly; I'm not good w/accents) when i sip this! I subbed Srpite Zero for club soda and used Diet Peach Sweet Tea. I wanted a big sippin' drink, so I used a bit more of each to fill my quart canning jar "glass". It's a nice sipping drink that with 2+ shots, packs a bit of a punch. CHEERS!
Any way to make this "Shirley Temple" mint julep
2 Ratings
* Percent Daily Values are based on a 2,000 calorie diet.
Southern Addiction Mint Julep
Serving Size: 1/1 of a recipe
Servings Per Recipe: 1
Amount Per Serving
Calories: 401
Calories from Fat: 1
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The firm, juicy texture of zucchini make them a perfect side to toss on the grill.
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Watch a professional bartender mix up a classic Mint Julep.
An icy cold bourbon cocktail with fresh mint and mounds of crushed ice.
This refreshing juice stars peaches, apples, lime, and fresh mint.


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Home > Chennai Bars & Pubs > Chennai > T.Nagar Bars & Pubs > 10 Downing Street, Kences Inn Boutique Hotel
54 Reviews / 64 Ratings
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July 25,2015
Good food and Great ambiance
I went there once for a karaoke night with friends and we had a really good time.
We ordered a few beers and the food was pretty decent, nothing great.
The service was good though I think because we were a large group.
But the place is quite expensive on the whole and if you are looking to go there on a Friday night and if you like drinking, then it is not the place to go.
March 09,2015
The party was organized for me on the performance for last month. I always knew that the place is filled with crowd and also for the amazing American Interior with cozy furnishing. But when I actually explored it, I loved it more than what I knew. Had an amazing combo meal with amazing liquor. For me a amazing place with decent price if you party often. 10 D thanks for making my beat up party successful. Lots of Love Pronita
first visit
Hi iam curious to visit 10D.iam hearing lot of positive reviews about this place. Iam a 35 yrs old lady want to have a lonesome drink and dine experience. Is it safe to do.could someone advice please.is there any particular dress code to be followed.any particular prior awareness required.
October 30,2013
A Loyalist
Ever since I discovered the 200 Rs lunch meal at 10 D, I have been a major regular almost every alternate week or so at 10 D . Its a complete value for meal ,and no compromise on taste or quality.
The must trys include the spinach corn lasagna combo, Butter Naan Paneer Butter Masala combo and the Thai Masala curry combo ( pretty much everything) . Their service is great and you can spend an eternity during lunch with some great music and awesome crowd !There might be several lunch places offering competitive priced food, but none can match 10 D for their quality, ambience and crowd
July 03,2013
A Fun Place To Hangout With Friends
I have been to this place a million times. Its a very good place to hang out with friends though they don't have much options in Beer. The lunch is very delicious and my all time favorite is Shredded lamb in the Thai section. The service is normal but sometime if you are lucky you will have a friendly waiter. Heard the evenings are good. Probably will try it out soon.
June 19,2013
A Wonderful Pub In T.Nagar
10 Downing street (true to its name,ironically) is a wonderful and a delightful pub(it serves a complete four course meal too,like a restaurant) located in the heart of T.Nagar , near Pondy Bazaar.
Its one of the very few pubs which is always brimming with people , be it a Monday or Friday or a Saturday night . The key feature in the pub is the lunch combo ( 170 for Veg and 220 for Non Veg) and a definite delight for any value meal hunters.
Don't miss out on the Friday , Saturday nights, though I'd argue that the Rs 1500 couple entry may be a bit steep.
June 17,2013
April 17,2013
Deepesh Tarun , Quality Analyst
April 08,2013
The Real Deal !!
There are two sides to this place. One is the lazy dine in place for lunch and the other a loud happening place at night.
The lunch combos are awesome and at a very standard price. The drinks if ordered properly are cheap ( not trying the cocktails and foreign liquor ), The ambience is also perfect. Very good for a relaxing drink or two and good food.
The nights have variety. Each night has a different theme. The Retro Fridays are relaxing. The Karaoke thursday are different and one of a kind in the city. The Ladies Night on Wednesdays and Club Nights on Saturday are extremely loud and noisy. The crowd is huge and the dance floor is absolutely cramped. And the bouncers will not let you in without a girl ( no escaping that). So if you do not like noise and the crowd, its definitely not advised to go on a Wednesday or Saturday night. But otherwise its a decent place for some good music and good food.
March 31,2013
Awesome lunch combo!
Visited this place after coming to know of their lunch combo.
The place is really well done. It has a good feel/vibe to it.
The lunch combo was really good for the price (around 200 if i remember right). You are given a main coure, a dessert and an option between a soup and a beer.
Not being a drinker, opted for the soup.
For the main course went in for the pasta which was cooked perfectly and the sauce was good too.
The service needs to improve.
Will I be back? Oh yeah. If not for anything else, atleast for their combo meals.
March 24,2013
Reopened with a bang
10D had a minor closure after a fire accident recently a month back
however they have opened up in style and has made the decor really well just like old times
The place was already packed yesterday and the DJ was spinning some great tracks
i am sure its' gonna take a few months time to get back the old flavour but if they seem to maintain the service and quality standards it should be in the leading club in no time.
and for lunch do try out their combos.. very reasonably priced and contains a set meal of a pint(or soup and dessert), main course and a side dish
March 23,2013
March 21,2013
Nothing great about it
Each visit to 10D Chennai has always left me asking myself (and others) - "What's so great about it?"
The first time I went there was the only time I was entranced by the decor and the yummy butter garlic prawns. The music wasn't too bad either. Follow it up with second visit, and I make the mistake of ordering a cocktail pitcher. Now I'm not much of a drinker, so you can understand how amazed I was at the light taste of the cocktail and how non-drunk I was even after finishing it. Conclusion - seriously watered down drinks. The bill was the other shocker, making me wish I'd gone to a 5 star hotel pub instead which would've had better drinks and a much better crowd.
March 19,2013
Cool place with a punch!
Located in T.Ngar, it's known for its laidback atmosphere and great ambiance.
Sufficient parking available and the service is swift and the waiters are friendly here.
They have a vfm combo offer for Rs 200 .
Maybe they can improve in variety, but overall good standards for food.
The food is really tasty and of good quality.
Go there to have a good time !
March 16,2013
December 20,2012
One of the best and happening discs in chennai , 10D is always a reliable place for a night of food,drinks and dance. They are pricey and actually much expensive than most of the clubs in chennai , but they do maintain their standards.. They have a decent amount of space and attracts a decent crowd too..
P.S. Dont miss their lunch combos.. Best value for meal ever !
November 17,2012
Nice place to hangout with friends!
The highlight is their combo offer for just 200 bucks! I love the au-gratin they serve! the shooters are good but the drinks arent strong enough, dunno why! the beer they serve is of good quality but its not the same with the cocktails! Waiters are a bit haughty but yeah a nice place to hang out with friends because they dont make a big fuss about dress code and stuff in the afternoons. I suggest they serve the delicious main course too during nights and not just the starters so that we can come for a full dinner with drinks at the side!
November 15,2012
Don't go there just for the alcohol. The lunch is a great steal at about 250 bucks, you get to choose from a fixed Indian, Chinese or Continental menu. Really worth the money, they give you either a beer or a soft drink free. The food is really filling and very tasty! The waiter could really do with a meeting with the Joker from the Batman series as a it would really be good if they could have a smile on their faces. Decently priced during lunch, very tasty food the ambiance is also a lot of fun with very young and vibrant people coming in daily. The service could definitely get better though.
November 10,2012
English Pub Feel
1st look - Enter this place and it reminds me of a pub in the UK.
Been to this place quite a few times, so this review is a mixed bag of all those experience. One of the happening pubs in chennai, if you are looking to just have a good time with some great music and good crowd, this is the place to be. Beware - Couples entry only most of the nights after 8pm.
Food - Must try is their Spinach lasagne. The tandoor food is also great - Paneer tikka, dal makhani, Paneer tikka masala.
Service is a bit of issue since this place is busy in the nights, else its fine!
Ambience - 4/5
Food - 4/5
Service - 3/5
November 09,2012
A Recent Favorite!
I simply love going to this place for lunch. It has a very laidback atmosphere and the ambience separates it from the rest of restopubs in the city. The lunch offer that they give you is substantial given their portions, which are massive. One dish and youll be set for six hours straight. I had the Spaghetti with Minced Lamb and I can say that it was definitely worth it because I couldnt get out of my seat for half an hour and kept delaying my friends from leaving. It can get pretty crowded up around 1 to 2, but the service remains quick, which is another thing I like about it.
October 01,2012
Sticks to the hype
Most ppl would say that this is place that is like really happening in Chennai. i don't think i would disagree much. yes this place has gr8 ambience. go there mostly on weekends for dinner. during weekdays its less drama there. the food and drinks are costly and on the higher side but i guess it gives us a reason to spend on a weekend and enjoy with friends. but do not expect good service. your orders will take time and u mite have to wait for a long time before you have a grab..!! Enough said..this place is good..!! but certainly not the best...!
April 06,2012
Such an unfriendly service
This place has some mean and rude waiters who doesn't care a damm about you. Those waiters attitude is like "Dude, you can walk away if you don't like the service I don't give a damm about it, cos you are not the only customer, as we have plenty of other customers waiting"
Worth a shot!
Total worth the money!
150 bucks for a veg meal(that includes a soup and a dessert) which tastes good is pretty darn good! !Loved the soup! The vegetarian pasta was really good! Kulfi wasnt great, but pista scoop was niccee!
The pub special mocktail was exquisite!
Was quite in a dilemma to visit this place (being a veggie and not a beer-type person).
But wasn't too bad! Loved the decorations and the interior designs & colours!
Divya Jayaraman, Senior Editor
February 21,2012
February 18,2012
A place for all your dull days! :)
It is truely an amazing place with very nicely done interiors and service. The manager and waiters are also friendly and ready to help at all times.. Thi splace has never failed to disappoint me till date!!
Crowd is fun and happening.. totally worth a visit on ladies night and weekends.. it will definitely cheer you up after a hard weeks work! DJ plays some real good contemporary music.. but for all the people who want a quiet place .. i suggest you stay away.. cos this place is full of people.. all live and kicking! :)
December 29,2011
December 08,2011
The Powerrrrrrr Lunch
I possibly cannot count the number of times I have bin to this place for the power lunch. Pocket friendly for a student like me..
To start with, I like the ambience. I generally prefer dark settings to bright, extremely well lit places(though they are good once in a while).
I also like how there are so many books aesthetically stacked. I usually reach the place early, grab a drink, take a book and just enjoy the nice music until the chaos of college friends arrive.(so much for the dull lighted ambience ;) )
Regarding the power lunch, their white sauce pasta is probably one of the best things there, and irritatingly so, it is never available. Whenever we order, the waiter says"Can I get Red Sauce?" Hell NO!! where is the white sauce???
So if you are lucky, the white sauce Pasta is good.
Their black daal is also pretty decent.
We never ventured too much into their continental dishes but surprisingly, one of the fried rice with some cauliflower whatever, is mouth wateringly yummy!
Their spinach corn lasagnia is also good, though a tad too sweet..
Anyway, Love the concept of power lunch here.. its just 150 bucks for the Soup/Beer+1 main Course+ice cream..
ps- pathetic service. make sure yo keep catching the attention of the waiter atleast a dozen times for him to respond once!
Mohammed Rawoof, Software Professional
November 12,2011
Ripped the floor apart...
I had been to 10D somewhere in april... Of all the places I have been in chennai, this place lives up to its hype n popularity... The place offers really good choices in food, drinks, shooters and cocktails... Heading down to 10D to dance the night away and party like hell... U're on the right path mate...
Heard a lot about TDS....Everyone said it was happening.. Went the first time as a couple. Got a corner table, had to beg the waiter for the food and drinks. Thought since we were in the corner , it was difficult for the waiter to see us :)
Went again with another couple, this time we hardly noticed the poor service as we ordered EVERYTHING on the first go...Hated the DJ..HE said no to every request we had, though we told him it was one persons bday...Wasnt obliging AT ALL..
I thought , this was it...Then i went there last weekend with 4 guys and me a gal...Went early and it was Sun nite so I guess we got in ok...We had fun..The music was good, the service was OK (we gotta beg these guys for our drinks and food) and overall the bouncers didnt disturb us a lot..
Overall guys, its good if you go as a group and there have to be girls, the bouncers are very fussy about stags and shoes...Parking is a BIG problem on Ladies nite..
I dunno why i like this place..prob cause i always have a good time, excluding the service
June 12,2011
10 Downing Street - always a pleasure!!
I've been to this place so many times and every time i come out with a more than satisfactory experience. The service is good, the ambiance is nice, the music is fine and the food is just great!! their non-veg tandoori platter is always beautifully done with finesse and their happy hours menu is very tempting!! one of my fav. places in Chennai...does not burn a deep hole in the pocket and great in experience for the mind and the tummy! :)
March 19,2011
10 Downing Street Rocking
Hai Guys after seen all reviews we went to 10 Downing Street for how they response but it's rocking guys we are 4 Guys & 3 gals went there nothing is wrong
& we got verry good response ( we expect Bad response B'cos of all reviews )
we can give 5 star nothing is wrong with the place check that profile people
Never again..
Visited this place 2-3 times earlier with bunch of friends and cousins but vow never again. The bouncers are very rude and have no idea how to treat customers.
On Feb 20 we visited this place along with friends and cousins just because it was a Bollywood night. On the dance floor the bouncers started abusing people stating that only couples were allowed and it didnt matter if you were in a group or not. They started abusing females who tried to explain that there were no sign and that they shouldnt allow stags if that was the case. On our way out he threatened us physically not knowing the consequences.
Bike & Barrel is lot better and worth every penny.
April 10,2010
Rotten service
Had been a regular to this place for several years, but never again after today. Booked a table for 8 for their "executive lunch" well in advance. When we showed up on time, we were escorted to a table that could barely seat four. We insisted that they give us another table, they persistently refused. Mighty embarrassing, especially as two of the party were visitors from overseas. Go there if you like food and give two hoots about the service. If service is important to you, avoid like the plague.
February 23,2010
Below 1 star
Go to this place only if:
- you want to deal with rude bouncers at the entrance on a Friday evening at 6.45pm (which to me is early enough to be giving me attitute) itself
-rude waiters who will take 30 minutes to bring a peanut masala and yet if you try to cancel the order after 30 mins, he will say that you cannot cancel it since it is already been prepared.
- this is by far the worse chennai addition, and truly doesnt deserve it. I the management doesnt take note of this seriously, they willl lose important business, I assure you!
Absolutely no customer service and will bend backwards to entertain the foreign (white) customer and yet in all honestly I will be spending wayy more than a foreigner, but they dont realize or understand that.
DONT ever go to this place, it can be totally skipped. Go to Bike N barrel instead just down the road, and not pretentious like this place is, you are much better of having a good time, without being stressed out.
Dilip Muralidaran, Sr. Tech Instructor
December 24,2009
Just for the ladies
This is not the first time it happens but let me write it down here anyways, for the women who want to visit 10Ds on Wednesdays because its ladies night and you can enjoy a free vodka + orange juice, or rather you foolishly think so.
Here is what i recommend:-
1. Sit at home
2. Drink your own Orange Juice (without vodka) in case you don't have vodka.
Why? Because spending fuel, time & resources in planning to take your ladies here is a total waste because your vodka (the free one) will not contain vodka but only orange juice diluted to the point of not finding one atom of orange in the juice.
This is not just it. When my friend asked the waiter to bring the vodka and the orange juice separately so that she can mix it herself, he rudely refused and said that "we serve only mixed drinks".
Finally we worked around the problem with these pricks by asking for vodka on the rocks. Guess what? Water for most parts and a tinge of the kerosene smelling Romanov cheap ass vodka, diluted again!
My assumption is the bartenders (if there are any) have multiple Ph.D's in under pouring free drinks. While under pouring free drinks is not bad, over doing it to the point of serving water is totally lame.
If you are a guy like me and you don't want to feel embarrassed like hell, trust me on this one. Don't take your girl friends to this place. Instead be rude to them, they may probably not mind or take it to heart but insulting their intelligence by taking them to a bar that is serving shitty drinks on a ladies night is not a perfect idea of a nice evening outing with friends, i could definitely say.
It just doesn't stop there. If you order cocktails like me, forget it. It's shitty and probably mixed by a janitor the way Phenyl is diluted with water before washing the restroom. For the price you pay here, just pay Rs. 30 more on your drinks and walk 20 meters from where you are and get into Bike & Barrel at residency towers. They serve real liquor mixed by real bar tenders that are priced & served just right. Plus, bike & barrel does not have any cover charges for men.
December 03,2009
July 23,2009
Go to the bike and barrel instead!
I've been to 10D more times than I can remember ... every time I go I'm amazed how rude, dishonest and aggressive the staff are.
When you buy a drink at the bar make sure that you check you change ... the barmen like to 'tip' themselves. Don't go outside to smoke, you might find that the doormen won't let you back in.
I've asked to see the manager two of the times I've been there, the first time I was told that there was no manager there that evening, the second time I was pushed out of the door by a bouncer.
There are only two good things about the place. Relaxed couple rule, a group of guys can get in. Karaoke on Thursdays. Any other night of the week you will have more fun at the bike and barrel.
July 16,2009
Cockroaches!! Help!!
Ok..the ambience is good..food is decent..music is awesome..but cockroaches? i'm talking abt the small kitchen variety..
i scrambled across my plate as picked up the last piece of honey chicken and another scurried across the wall behind me!!
So wat will i remember abt 10D? not the delicious salt and pepper prawns or prawn sesame toast..but the COCKROACHES!!
Arun Krishnamurthy, Team Manager
July 14,2009
Super place to chill...
This is a great english pub to go with your friends and have a chilled drink with some awesome side eats!! The pub is quintessentially british and the ambience is simply perfect. I am there almost every thursday night when the 'karaoke' nights happen!! Quite simply one of the best days to be in the pub!! Theres lotsa singin, dancing and no one shies away from the mike after downing a few ones!! Its great fun to listen to people croon their hearts out and the pub generally has a gala atmosphere on that night!! I would recommend this place to anyone who wants to have a good stressbuster on thursday nights!! They have a good power lunch menu for the noons that is easy on the pocket and is good value for money. Wednesdays is ladies night from 7.30 to 9.30, so the fairer sex can be seen in abundance then!! They also have classic rock playin on fridays, so its great fun then too!! All in all a great place to hang out with friends and cool off!!
May 24,2009
Sing Your Heart Out
Went there on karaoke night! Wow, what a smart decision! We had a blast singing not only to the songs we chose, but also singing along with others who graciously and enthusiastically called us up to the front to sing along! The drinks were flowing, and we stayed till closing time. Awesome place to chill with a group of friends who know how to have a good time! Just wish every night could be ladies night ;)
Ps. Woke up with a sore throat and squeaky voice, but it was so worth it!!!
May 22,2009
What the ???
I am here in India for a couple of months and just wanted to get a drink last Friday and someone suggested this as being near my place so I stopped by.
And the guy outside goes- sorry sir couples only..I said- I just wanna get a drink - I mean Seriously I was all well dressed and did go in a car- What are these guys expecting we go to a bar and do?
And then I walk into Bikes and Barells and they have only one variety of Red-wine! and seeing I am alone he asks me to go upstairs- where its all a bunch of guys standing...
Seriously- what are we??
Finally ended up going to Leather Bar- found some people to strike a conversation and some good beer!
April 11,2009
Ambient Pub
I went out to this place on Ladies' Night and, boy, was it rocking! There were absolutely no problems in entering. Guys pay for entry. Girls enter for free. Drinks were on the house for the gals from 7:30 to 9:30 only though so be there on time if want to take true advantage of the night, for they set their clock 10 minutes fast.
The drinks are a bit on the expensive side. But the bartenders are good and will mix up whatever you ask them to.
The dance floor is pretty small. But that is to be expected for this is a pub after all. The ambiance is British comfort, with books on shelves and pictures and a sign of London streets.
They also offer a Power Lunch from 12 to 3:30 for 150/- and 200/- for veg and non-veg respectively. This comes with a choice of beer, soft drink or soup, a main course and dessert, in continental, indian and oriental.
Would definitely revisit this place soon.
April 10,2009
Good place BUT
Have been here some 2-3 times. The booze is good and their cigars are also cheaper. The food can be ranked average. It's a smokey place though and has a good ambiance. I lvoed the music played whenever I had been there.
Being a chennai-ite I would have been expected to have visited this palce with so much positives more than 2-3 times. But it's something called self-respect [yeah some call it ego] which doesn't let me to. Their handling of stags is what I am speaking about.
If it's a couples-only night and you are there without knowing it...that's the end. The manager as well as the bouncers treat you like a serial-rapist on the loose. There are many pubs which do turn away stags but the most rudest [n obscene]way of doing it is here...It may have be a one off incident but then that overrides all the good time had o'er there.
Not a good place for office folks!
Me and my pals went on a Wednesday night at 10 Downing Street (TDS), Chennai. It was supposedly a Ladies Nite as declared by the TDS. But guess what, ladies night is only from 7 pm - 9.30 pm Who goes to a pub this early on a weekday??? !!!! Its a smoky loud place. Definitely not a place to chill out after a hard day at work. Recommended for college folks!
September 01,2008
Good place to chill...
I make frequent trips to Chennai, and 10 D is definately a regular on my Itinerary. There are many good reasons for this;
good ambience, pretty cool crowd (not only teeny boppers), fairly good music, good central location.
I do believe they come up with pretty good promos from time to time..eg. ladies night and then one night where you get a discount equivalent to the age of the oldest person on the table (eg. if the oldest person is 60..you get an amazing 60% discount on alcohol!).
Also, they have a good concept of retaining clientele... you can buy a bottle of whisky/vodka etc and consume only as much as you want. They'll tag your bottle and you can just request for it the next time you go there. This is cool because a large (domestic liquor) would come to approx Rs.300/- and an entire bottle is comparatively a lot more economical at Rs.1500/-.
As far as the food is concerned, although the non veg platter is decent... if you want to have a really good laugh, you must order the non veg club sandwhich. It is the biggest joke of a club sandwhich I have ever witnessed. I can't explain, you have to see it to believe it... at your own risk cos it's definately not worth it!
August 06,2008
Now that's what I call a pub!
What a relief to dying chennai's nightlife! Something with class is here on offer, right from the entry where they make sure to check your ID, to real-time bouncers, to the dark typical classic-pub ambiance with secluded booths. I rem many places that opened up with class but went down the drain soon, losing sophistication. Somehow 10D seems promising enough to keep it going.
Perfect, is the word wen u look at the menu. An awesome lot to drink and eat. The cocktails don stop with the regular bloody mary, screwdriver and long island icetea like many other pubs in the city. They have innovative drinks and the cosmopolitan is to die for. They have specials everyday. Ladies drink most drinks on the house on wednesdays.
The service is not bad, but doesnt reflect the standards of the pub. The waiter was a lil weird n non-professional, also we never received an item we almost asked for 10 times. There is no snacks on the house! But for the food, the potato wedges and the chesse sandwich are awesome! Heard the non-veg is good too. They have a pub lunch and m guessing it shud b equally gr8.
June 17,2008
Bole Toh Britsh Sthyle
well...ten....is really a best place whn in comes to pub i went...i had been here many times...especially this place s so lively on wednesdays and weeknds..wednesdays wow the best ppl of chennai cums here damn good crowd which reminds me of shisha (kolkata) wic is the best place thr..where im regular since here i miss my nite life as still chennai lacks lot thanks to our govt.
but ten jus reminds me of so much v good music nice dj. ya the place is just 2 small that u gets bump in with a person ..its basically cosiest place.well starters are good and drinks are expensive but nothing comes 4 free so there is a saying all gud things cum in pakage ...but som bad exp also i had when i went there 1st time...as the bar tenders are really take advantage they make the rong billing but thanks to the manager things solved. over all the place is v lively lovely music drinks sexy babes and wat else..this place is to chill in.
Shashank Aggarwal, Cheif Food Enthusiast
June 16,2008
not what I am used too
Went to this place over the weekend, however it over my definition of a pub or disco. Or maybe we were there before the 'time'. We actually went in around 8 or 8:30 pm and came out just after 10 pm.
It seemed to be a mixed bag to me, the are where we were sitting, seemed a bit tacky to me. crowds were just in extremes. Like on one hand, you had this group of 40 somethings, and on other you had good gatherings on 20 somethings. Food was just average, drinks overpriced (maybe its just Chennai effect).
And just wanted to comment on the review below, as the person has mentioned music was too loud, and they were not able to converse there, but I guess this place is supposed to this way only, can one cannot expect to go there and hold a conversation.
June 14,2008
Keep High Spirits!!
Just been to this place with some colleague's, had a nice time enjoyed a lot.
I ordered Absolute with orange juice and tequila.
The music was too loud to even talk. The tables were too small and set up too close to each other..
The service was good and quick too..
Just Before we left we were made to feel like celebrities.. The Deccan Chronical reporters took our pictures, to be featured in the papers..
I would rate it as an evening spend well..
June 05,2008
A true pub in the English tradition
10 Downing Street is a bit of a surprise when you first enter (if you can push yourself through the crowds thronging) - this is the lighting.
A more dim place I have yet to see at Chennai, but you almost forgive them the lack of lighting when you look at the decor - it's original in that they have not tried to "indianise" an essentially English pub theme. I really cannot comment on their cocktails - I have never had them here, but I can comment on their ladies' night - unlike the other places, here all drinks had by the ladies are on the house (every Wednesday) and you can see that they are quite popular with the ladies then (free drinks limited to certain brands of course).
I tried the Tandoori Platter as well as some corn and they were pretty good and music is excellent.
For those who want to do a pub lunch in Chennai, these people are open at 11 AM, so go ahead and indulge yourselves. They are the closest thing you would get to a real pub in Chennai as per the English definition of the word "pub".
Unfortunately, their parking leaves a lot to be desired and I had to get the manager to intervene and help us each time we were there.
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I have used Bible Promise for many years, and it is exactly what I want, but I have just changed computers and now it is not keeping the info that I have put in for a registered copy and I have to put the details in at every boot. I might be able to sort it, but if not, what is there out there which you might recommend?
Edited by pawz, 02 July 2014 - 04:25 AM.


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NEW YORK — As Cam Newton proudly held the Heisman Trophy, many people still wondered how his reputation would hold up over time.
Less than 30 minutes after winning, Newton was put on the spot at his press conference. He was asked point blank if he worried about one day having to give back the trophy, the way 2005 winner Reggie Bush did earlier this year after being tied to NCAA infractions.
"Two letters for you my friend," Newton said, staring his questioner in the face. "No."
If Newton is right, those who voted for him will be relieved.
Newton captured the Heisman by a landslide Saturday night, and Auburn's star quarterback was clearly the most dominant player in college football this season. Yet, he remains a controversial winner after the NCAA ruled Newton's father tried to engineer a pay-to-play scheme that would have sent his son to Mississippi State.
With no evidence that Newton, or Auburn, knew of his father's scheme, most voters felt it would be unfair to penalize Newton.
"Sure there are concerns, but we can't foresee the future, and we don't know what the future holds," said 1992 Heisman Trophy winner Gino Torretta. "We're just going by what we know right now. The NCAA says he's eligible, so he's eligible."
Newton also says he is innocent, and if anyone knows otherwise, they should come forward. In the meantime, unless more damaging information is uncovered, Newton deserves to keep the award.
But hopefully this stressful experience has taught him some valuable lessons.
The controversy surrounding his father cost Newton and his father the chance to share Saturday night's moment together.
Cecil Newton stayed away from the Heisman ceremony, not wanting to be a distraction. That is a moment they cannot get back, and Newton admitted he was hurt that his father was not by his side in New York.
Newton has definitely shown his resilience during his ordeal, which bodes well for his ability to handle adversity moving forward. He led his unbeaten Auburn team to inspiring victories over Alabama and South Carolina, while people all over the country debated whether or not he deserved to win the Heisman.
However, people will continue to judge Newton from a afar — a price of fame that Newton had better be prepared to deal with.
One day, Newton wants to be the face of an NFL franchise, and daily scrutiny will come with the territory. Newton handled himself well Saturday night. He looked poised and honored, accepting the trophy with humility as cameras flashed non-stop.
"When I embraced my mother, I really didn't want to let go,'' Newton said. "It's been hard for me, but it's been extremely hard for her, just to see how much her son has been through. I just wanted to hug her the whole night, make it feel at ease. It's over, for this particular moment of our lives."
However, Newton's career is just getting started, and winning the Heisman makes him a part of college football history. America has a fascination with this award, which is why the trophy presentation has become a prime-time television spectacle.
Many people also want their Heisman Trophy winners to be heroes, and Newton basked in the glow, walking through a Manhattan hotel lobby as people shouted his name.
Saturday night, Newton was sitting on top of the college football world.
Let's hope 20 years from now, the Heisman Trophy is still sitting on his mantle.


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Enter your information and a representative will contact you for this 2011 Mercedes-Benz CLS-Class !
Mercedes-Benz calls its CLS-Class a "four-door coupe" It's technically a sedan, but its stance and sporty appearance do create the look of a coupe. The CLS-Class is a high-performance luxury car that's available with one of two V-8 engines; the larger, with the optional Performance package, makes a top speed of 186 mph, according to the manufacturer. The 2011 model is mainly a carryover from the previous year, but some previously optional features have been added as standard.
The two trim levels for the 2011 Mercedes-Benz CLS-Class are the CLS550 and the CLS63 AMG. The base CLS550 features 18-inch cast aluminum wheels with high-performance tires, adaptive air suspension, sunroof, 4-zone automatic climate control, leather upholstery, hard-drive-based navigation system, 13-speaker Harmon Kardon sound system, and a long list of power accessories. Nineteen-inch wheels are also available. Standard on the CLS63 AMG are 19-inch wheels, a sportier exterior, special brakes to go with its V-8 engine, plus upgraded upholstery, AMG-tuned sport suspension, and sport front seats. Various option packages can add larger wheels, limited-slip differential, a premium Bang & Olufsen surround-sound system, track-calibrated suspension settings, and many other features.
Standard on the 2011 Mercedes-Benz CLS550 is a 382-horsepower, 5.5-liter V-8 engine. The CLS63 AMG features a 507-horsepower, 6.2-liter V-8 engine, which produces 465 lb.-ft. of torque. Both engines work with a 7-speed automatic transmission and rear-wheel drive. According to the EPA, the 5.5-liter engine delivers 14 mpg in the city and 21 mpg on the highway; the larger engine delivers 12 mpg in the city and 18 mpg on the highway.
Standard safety features on the 2011 Mercedes-Benz CLS-Class include an emergency telematics system, anti-lock brakes, stability control, active front head restraints, and a full complement of air bags. Mercedes-Benz's Pre-Safe system closes the sunroof and braces passengers if impact is imminent.
Starting MSRPs for the 2011 Mercedes-Benz CLS Class ranged from $74,000 to just under $100,000.


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I was actually getting ready just before I read your always great How To articles Ally to send an email to 3 people so I tried your tip and sent 2 of them a BCC and it worked just great, Exactly as you said it would. Thank you as always for the tip. Invaluble info and I hope everyone new to iPhone and even myself reads your articles. They will learn what they might have never known before. Thank you.
How to quickly move recipients to different fields in Mail on iPhone and iPad
If you send a lot of emails on your iPhone or iPad that go to different people, you may want to blind copy some users, or all of them for that matter, so they don't see each other's email addresses. Regardless of the reason, if you forget there isn't a need to delete the email and re-enter it in the appropriate field. iOS actually gives you the ability to move them pretty seamlessly.
Here's how.
- Launch the Mail app from the Home screen of your iPhone.
- Tap on the Compose button in the lower right hand corner.
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- Hold down on the one you'd like to move to a different send field and simply drag it to the new field and release your finger.
That's all there is to it. This is a great tool for times when you need to blind copy someone and accidentally but an address in the wrong field. Try it out and let us know how it works for you!
Have something to say about this story? Leave a comment! Need help with something else? Ask in our forums!
Reader comments
How to quickly move recipients to different fields in Mail on iPhone and iPad
That's good to know. I've been using iPhone for a long time and never knew that. Just when you think you know everything there's to about iOS you learn something new lol.
Can't tell you how much this was well received when it was first announced at the iPhone keynote (I forget which one it was). I've used it more times than I want to admit. Thanks for reminding us.
Great tip. This will help me stop having to delete and re add the name. Thanks!
Great tip! I've been using iOS for years and didn't know this trick!
great tip very useful


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Video & Multimedia
ITS supports both academic and administrative departments with the creation of long and short-form video programs. We provide all aspects of video support, including full video production and post-production services, live event broadcasting, classroom recording, video distribution services, and video conferencing. Visit the video.wesleyan.edu gallery to view recent productions.
Video Production Services
The ITS New Media Lab (NML) provides production and post-production support for academic and university produced video programming intended for the Wesleyan website and social media distribution channels. ITS's Instructional Media Services (IMS) provides video and audio recording support used for internal purposes, including video archiving, classroom recordings and reference video. [Learn more]
Event and Audio/Visual Support
The ITS Instructional Media Services (IMS) provides special event and audio visual services support throughout the Wesleyan campus for events like lectures, dances, film-screenings, and debates. The service includes providing and setting up needed equipment such as projectors, screens, microphones, speakers, mixing boards. [Learn more]
Classroom Recording
Faculty may request to have their classroom lectures recorded and made available online for their students to review. [Learn More]
Live-Broadcasting: WesCastWesCast is Wesleyan's live-broadcasting service. We live-broadcast important events such as sports games, lectures, etc. so that anyone around the world with a computer and access to the internet can view your event. If you are looking to view a live WesCast event, go to www.wesleyan.edu/wescast. [Learn more]
The Office of University Communications (UComm) maintains the Wesleyan University YouTube Channel and can assist faculty, staff and students with uploading video content for public distribution. [Learn more]
iTunes U
Wesleyan University provides access to more than 1,000 faculty and guest speaker lectures and podcasts as well as special events and weekly seminars on its public iTunes U site. [Learn more]


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The rivalry hasn't been dormant for 29 years. We played against Seattle since the PTFC restarted in 2001. This was the first fixture in league play since the scum went to MLS in 2009.
Redfly I pity your sad sheltered life.
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The importance of non-reportable income to Middle America
By Jeff Reed
Kestler Financial Group
The reality is that high income earners are not the only ones affected by means testing and the recent changes in tax laws under Obamacare.
In fact, when you start to look at means testing and the income levels at which Social Security and Medicare benefits are impacted, these changes may be more meaningful for Middle America than for those with high net worth. We've already explored the tax increases that the wealthy face in 2013, and when you add in the phase-out of many deductions based on PEP and Pease, it's clear that the wealthy are taking a big hit.
But when we look at the magnitude of the change in real dollars, like the loss of the $3,900 personal exemption over $372,000 in income, are the high-income earners really going to care?
I think they care much more about the tax rate increases. These other items, while still worth mentioning, are but another layer on a cake they already don't want to eat.
When our focus changes to those who are going to have a more marginal retirement, some aspects of means testing become much more significant. Taxation of Social Security benefits, for instance, begins at income levels as low as $44,000. Similarly, Medicare costs begin to increase at an $85,000 income.
The impact on Social Security
To gain a deeper level of understanding on the issue, let's talk real dollars, starting with Social Security.
If Middle American clients are maximizing their Social Security benefits, they can bring in roughly $30,000 per year. If they also have income from other sources, and their income totals $100,000, they're going to pay tax on a portion of their Social Security income. Sure, tax is taking a smaller bite out of their income than that of a wealthy, high-income client, but look at the significance of those dollars. To the high-income client, taxing their benefits is a nuisance. For the average client, however, paying an additional $10,000 in tax can become a big problem.
The impact on Medicare
Medicare costs are much the same.
Rather than benefits being subject to tax, Medicare costs rise if an individual has "too much" income. Sounds ominous, and no one is excited about paying more. However, when we look at the increase in real dollars, it totals $3,900 per year.
Again, not an insignificant amount of money, but much more meaningful to the average Joe than the high-net-worth client. Life insurance as a source of income
Taken together, these two issues can spell trouble for a retirement plan. The tax benefits of using life insurance as a source of income in retirement are already well known and become increasingly important in a period of rising tax rates.
The other feature of income from a life contract — the fact that it's non-reportable — may make life insurance a critical component of an effective retirement plan in the new economy. Tax efficient income that preserves benefits from social welfare programs could make the difference between a client's comfortable retirement and a client outliving his money.
For the average client mentioned above, saving him even half of the taxes paid on his Social Security benefits and reducing his Medicare costs could increase his net income by $7,000. I'm certain that the 7 percent increase will be very meaningful to him.


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Miss Movin' On
Redirected from Miss Movin On
this wiki
Miss Movin' On is the debut single for the American girl group Fifth Harmony. The single was released on June 15, 2013 on Saturday Night Online and is the main single on their debut EP, Better Together . The single was made available through iTunes on July 16, 2013. The girls announced on Twitter that the single was certified gold by the Recording Industry Association of America on June 17, 2014.
The main producer of the debut album, Julian Bunetta, said on Twitter that two singles from the Debut Album are coming on June, resulting in the expectations by fans growing. On June 4, it was announced on Twitter that there was going to be an announcement related to the single. On June 7, 2013, the girls announced by a video on Vine that the debut single is called "Miss Movin' On". Harmonizers trended on Twitter "#WeWant5HMissMovinOn", after that the girls released a little preview of the song. It should be mentioned that a few months ago, before this song was announced as the debut single, there were already rumors on internet saying that "Miss Movin' On" was the single.
The song was released through SaturdayNight Online. It was released on iTunes on July 16, 2013 and the music video was released on July 15. The girls performed it on The Today Show on July 18, 2013 along with Me and My Girls.
Chart Performance
Following it's' release on iTunes on the 16th of July, "Miss Movin' On" reached the Top 10 in 16 countries (El Salvador, Peru, Honduras, Costa Rica, Dominican Republic, Singapore, Venezuela, Argentina, Nicaragua, Israel, Ecuador, Paraguay, Malta, New Zealand, Malaysia and Guatemala).
The song debuted at #85 on the Billboard Hot 100, becoming the highest-peaking single by any X Factor USA contestant.
|Chart Name
|Peak Position
|US Billboard Hot 100
|US Billboard Pop Songs
|Radio Disney Top 30 Countdown
|New Zealand (Recorded Music NZ)
|US Hot Digital Songs (Billboard)
|US Heatseekers (Billboard)
- Fifth Harmony posted four lyric previews on their tumblr, facebook page, and twitter.
- The song was released through SaturdayNight Online.
- Although Camila and Lauren deemed as being the most popular from the group, many harmonizers were very upset that Ally and Normani had very little singing time. Some say this is the "One Direction Problem" all over again, where it was Niall and Louis that rarely sang.
- Harmonizers are encouraging each other to buy the song instead of illegaly downloading so that they can support the girls.
- Harmonizers are encouraging each other to call and tweet top radio stations like Z100 in New York and request the song.
- The song was originally supposed to be released on June 18, 2013, but was later announced to be released on July 16, 2013.
- A radio station said the Fifth Harmony fandom is the "most annoying" because they have so many requests to play the single.
- Ally sings Lauren second verse on every live performance.
- The girls performed the song on NBC's The Today Show on July 18, 2013.
- The song's music video has racked up over 5 million views and counting on VEVO since it came out on July 16, 2013. The video's views hit the 1 million mark 3 days after the video came out.