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MAB424 | Anti-PCNA Antibody, clone PC10
Use Anti-PCNA Antibody, clone PC10 (mouse monoclonal antibody) validated in ELISA, FC,IHC(P), IP, WB to detect PCNA also known as Proliferating Cell Nuclear Antigen, DNA Polymerase delta Processivity More>>
Use Anti-PCNA Antibody, clone PC10 (mouse monoclonal antibody) validated in ELISA, FC,IHC(P), IP, WB to detect PCNA also known as Proliferating Cell Nuclear Antigen, DNA Polymerase delta Processivity Factor. Less<<
|Replacement Information
Offres spéciales
Tableau de caractéristiques principal
|Species Reactivity
|Key Applications
|Antibody Type
|Inv, Vrt
|Monoclonal Antibody
|Product Information
|Protein A Purified mouse immunoglobulin in PBS with 0.1% sodium azide as a preservative.
|Physicochemical Information
|Materials Information
|Toxicological Information
|Safety Information according to GHS
|Safety Information
|Packaging Information
|Material Size
|500 µg
|Transport Information
|Supplemental Information
Certificats d'analyse
Références bibliographiques | 18 Disponible | Voir toutes les références
|Aperçu de la référence bibliographique
|Nº PubMed
|Inhibition of HIV-1 Tat-mediated transcription by a coumarin derivative, BPRHIV001, through the Akt pathway.
Lin PH, Ke YY, Su CT, Shiao HY, Hsieh HP, Chao YK, Lee CN, Kao CL, Chao YS, Chang SY
Journal of virology 85 9114-26. Epub 2011 Jun 22. 2011
Afficher le résumé
The human immunodeficiency virus type 1 (HIV-1)-encoded RNA-binding protein Tat is known to play an essential role in viral gene expression. In the search for novel compounds to inhibit Tat transactivity, one coumarin derivative, BPRHIV001, was identified, with a 50% effective concentration (EC(50)) against HIV-1 at 1.3 nM. BPRHIV001 is likely to exert its effects at the stage after initiation of RNAPII elongation since Tat protein expression and the assembly of the Tat/P-TEFb complex remained unchanged. Next, a reduction of the p300 protein level, known to modulate Tat function through acetylation, was observed upon BPRHIV001 treatment, while the p300 mRNA level was unaffected. A concordant reduction of phosphorylated Akt, which was shown to be closely related to p300 stability, was observed in the presence of BPRHIV001 and was accompanied by a decrease of phosphorylated PDPK1, a well-known Akt activator. Furthermore, the docking analysis revealed that the reduced PDPK1 phosphorylation likely resulted from the allosteric effect of interaction between BPRHIV001 and PDPK1. With strong synergistic effects with current reverse transcriptase inhibitors, BPRHIV001 has the potential to become a promising lead compound for the development of a novel therapeutic agent against HIV-1 infection.
|Oligodeoxynucleotide IMT504 induces a marked recovery in a streptozotocin-induced model of diabetes in rats: correlation with an early increase in the expression of nestin and neurogenin 3 progenitor cell markers.
MS Bianchi, A Hernando-Insua, NA Chasseing, JM Rodriguez, F Elias, N Lago, J Zorzopulos, C Libertun, AD Montaner, VA Lux-Lantos
Diabetologia 2010
Afficher le résumé
AIMS/HYPOTHESIS: IMT504 is an oligonucleotide that promotes tissue repair in bone injury and neuropathic pain models by stimulating progenitor cells. Here we evaluated the effect of IMT504 on the recovery of islet function in a streptozotocin (STZ)-induced model of diabetes in the rat. METHODS: Male Sprague-Dawley rats were injected with STZ (60 mg/kg, i.p., day 1) or citrate buffer (Control). Animals with glycaemia between 11 and 20 mmol/l on day 4 were injected with IMT504 (4 mg/animal in saline, s.c., STZ-IMT504) or with saline (STZ-Saline) for 10 days. Glycaemia and water and food intake were recorded for 33 days. Intraperitoneal glucose tolerance tests (IPGTTs) were performed on day 30. On day 35, overnight-fasted animals were killed and blood samples and pancreases collected for hormonal and histological studies. A second group of STZ-IMT504 rats was killed, together with Control and STZ-Saline rats, after two consecutive days of blood glucose decreases after the beginning of IMT504 treatment. Pancreases were collected and proliferating cell nuclear antigen (PCNA), nestin and neurogenin 3 (NGN3) detected by immunohistochemistry. RESULTS: IMT504 greatly improved blood glucose and food and water intakes in STZ-IMT504 rats by day 8, as well as IPGTTs on day 30. Significant increases in islet number and beta cell content were observed in STZ-IMT504 rats (day 33). Furthermore, after two to five IMT504 injections, blood glucose decreased, and an increase in pancreatic nestin (mainly in endothelial cells), PCNA and NGN3 production (in islets) was observed in STZ-IMT504 rats. CONCLUSIONS/INTERPRETATION: IMT504 induced a marked recovery of STZ-induced diabetes that correlated with early production of progenitor cell markers, such as nestin and NGN3.,
|RanBPM Has Proapoptotic Activities That Regulate Cell Death Pathways in Response to DNA Damage.
Atabakhsh, Elnaz, et al.
Mol. Cancer Res., (2009) 2009
Afficher le résumé
Ran-binding protein M (RanBPM) is a nucleocytoplasmic protein previously implicated in various signaling pathways, but whose function remains enigmatic. Here, we provide evidence that RanBPM functions as an activator of apoptotic pathways induced by DNA damage. First, transient expression of RanBPM in HeLa cells induced cell death through caspase activation, and in the long-term, forced expression of RanBPM impaired cell viability. RanBPM COOH-terminal domain stimulated the ability of RanBPM to induce caspase activation, whereas this activity was negatively regulated by the central SPRY domain. Second, small interfering RNA-directed knockdown of RanBPM prevented DNA damage-induced apoptosis, as evidenced by the marked reduction in caspase-3 and caspase-2 activation. This correlated with a magnitude fold increase in the survival of RanBPM-depleted cells. Following ionizing radiation treatment, we observed a progressive relocalization of RanBPM from the nucleus to the cytoplasm, suggesting that the activation of apoptotic pathways by RanBPM in response to ionizing radiation may be regulated by nucleocytoplasmic trafficking. Finally, RanBPM downregulation was associated with a marked decrease of mitochondria-associated Bax, whereas Bcl-2 overall levels were dramatically upregulated. Overall, our results reveal a novel proapoptotic function for RanBPM in DNA damage-induced apoptosis through the regulation of factors involved in the mitochondrial apoptotic pathway. (Mol Cancer Res 2009;7(12):1962-72).
|Spatial and temporal expression of RP58, a novel zinc finger transcriptional repressor, in mouse brain.
Chiaki Ohtaka-Maruyama, Akiko Miwa, Hitoshi Kawano, Masataka Kasai, Haruo Okado
The Journal of comparative neurology 502 1098-108 2007
Afficher le résumé
RP58, a novel zinc finger protein containing a POZ domain, is a sequence-specific transcriptional repressor. To understand the role of this protein, we examined RP58 gene expression in the developing mouse brain by quantitative polymerase chain reaction (PCR) and in situ hybridization. RP58 mRNA expression was detected at embryonic day (E) 10 in the neuroepithelium, and subsequently in the ventricular zones of the cerebral cortex in the E12 embryo. Strong expression was observed in the preplate in the cerebral cortex from this stage onward. High levels of expression continued to be detected in the cortical plate and subventricular zone of the neocortex, hippocampus, and parts of the amygdala, but not in the thalamus or striatum. These results suggest that RP58 plays a crucial role in neuronal proliferation, migration, and differentiation in the developing cerebral cortex. RP58 is also expressed in the adult mouse neocortex, hippocampus, parts of the amygdala, and granule cells in the cerebellum. Double in situ hybridization using GAD67 or VGLUT1 probes revealed that RP58 is expressed in glutamatergic excitatory neurons.
|Mitogenic effects of the up-regulation of minichromosome maintenance proteins in anaplastic thyroid carcinoma.
Teresa Guida, Giuliana Salvatore, Pinuccia Faviana, Riccardo Giannini, Ginesa Garcia-Rostan, Livia Provitera, Fulvio Basolo, Alfredo Fusco, Francesca Carlomagno, Massimo Santoro
The Journal of clinical endocrinology and metabolism 90 4703-9 2005
Afficher le résumé
CONTEXT: Anaplastic thyroid carcinomas (ATC) are among the most aggressive human malignancies and are characterized by high mitotic activity. Minichromosome maintenance proteins (MCM) 2-7 are required to initiate eukaryotic DNA replication, and their overexpression has been associated with dysplasia and malignancy. OBJECTIVE: In an attempt to cast light on the mechanisms governing ATC, we evaluated MCM5 and MCM7 expression in human normal, papillary (PTC), and anaplastic thyroid samples, as well as in primary culture cells and transgenic mouse models. RESULTS: MCM5 and MCM7 expression was high in 65% of ATC and negligible in normal thyroid tissue and papillary thyroid carcinomas. In ATC, high MCM5 and MCM7 expression was paralleled by high levels of MCM2 and MCM6. An analysis of human ATC primary cell cultures and of a transgenic mouse model of ATC confirmed these findings. An increased transcription rate accounted for MCM7 up-regulation, because the activity of the MCM7 promoter was more than 10-fold higher in ATC cells compared with normal thyroid cells. Adoptive overexpression of wild-type p53, but not of its inactive (R248W and R273H) mutants, strongly down-regulated transcription from the MCM7 promoter, suggesting that p53 knock-out contributes to MCM7 up-regulation in ATC. Treatment with small inhibitory duplex RNAs, which decrease MCM7 protein levels, reduced the rate of DNA synthesis in ATC cells. CONCLUSION: MCM proteins are overexpressed in ATC and sustain the high proliferative capacity of ATC cells.
|Histochemical and immunohistochemical localisation of elastic system fibres in focal reactive overgrowths of oral mucosa.
A J Mighell, P A Robinson, W J Hume, A J Mighell, P A Robinson, W J Hume, A J Mighell, P A Robinson, W J Hume
Journal of oral pathology medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 26 153-8 1997
Afficher le résumé
Eight specimens each of the following groups were investigated: gingival pyogenic granuloma, fibrous epulis, calcifying fibrous epulis, peripheral giant cell granuloma, giant cell fibroma (four gingival, four non-gingival), denture-irritation hyperplasia and fibroepithelial polyp. These lesions have diverse histopathological appearances but the composition of their connective tissue is poorly defined. The elastic system consists of a complex mixture of glycoproteins that in normal oral mucosa form three differentially distributed fibre types; oxytalan, elaunin and elastic. The elastic system was investigated by Verhoeff's haematoxylin stain, aldehyde fuchsin staining and an anti-elastin monoclonal antibody. Elastin was identified in all fibroepithelial polyps and denture-irritation hyperplasias, but in none of the other lesions. In particular, this identified a distinct difference in the extracellular matrix between the giant cell fibroma and fibroepithelial polyp. Many of the epulides included only oxytalan fibres, but the presence of oxytalan fibres did not follow any pattern within either a single lesion group, or between different lesions. However, the presence of oxytalan fibres in the absence of elastin does not necessarily support a periodontal ligament origin for reactive epulides.
|Immunolocalisation of tenascin-C in focal reactive overgrowths of oral mucosa.
A J Mighell, P A Robinson, W J Hume
Journal of oral pathology medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 25 163-9 1996
Afficher le résumé
Focal reactive overgrowths of oral mucosa were investigated in the following groups: gingival pyogenic granuloma, fibrous epulis, calcifying fibrous epulis, peripheral giant cell granuloma, giant cell fibroma, fibroepithelial polyp and denture-related fibrous hyperplasia (n = 8 for each group). We hypothesised that immunoreactivity to tenascin-C, a functional protein associated with connective tissue organisation and cell migration, would be differentially distributed in individual lesions and between lesion groups. Staining patterns for giant cell fibromas and fibroepithelial polyps were similar to those reported for normal mucosa. By contrast, additional staining was observed in the other lesion groups, although immunoreactivity was variable and not specific to each lesion group. Strong immunoreactivity was observed around blood vessels lined with plump endothelial cells and in regions where keratinocytes were migrating over ulcerated surfaces. Interlacing collagenous fascicles could be either strongly or weakly immunoreactive, with either fibrillar or diffuse staining. Localised staining was observed around, but not within, areas of calcification.
|PCNA and Ki-67 immunoreactivity in multinucleated cells of giant cell fibroma and peripheral giant cell granuloma.
A J Mighell, P A Robinson, W J Hume
Journal of oral pathology medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology 25 193-9 1996
Afficher le résumé
Immunohistochemical investigation of PCNA and Ki-67, two diverse nuclear proteins essential to the cell cycle, was undertaken in archival, formalin-fixed and paraffin-embedded specimens of giant cell fibroma (GCF) and peripheral giant cell granuloma (PGCG++). GCF multinucleated cell nuclei were mostly PCNA+, although there was variability in staining intensity. This indicates heterogeneity in nuclear PCNA metabolism of GCF multinucleated cells, and it is possible that the most intensely stained nuclei have passed through the cell cycle more recently compared to the less immunoreactive nuclei. However, the absence of Ki-67 immunoreactivity in GCF multinucleated cells, and absence of mitoses in GCF multinucleated cells, suggests that cell cycling in the absence of cytokinesis is not involved in GCF multinucleated cell formation. Alternatively, GCF multinucleated cells possibly form by fusion of mononuclear cells previously identified as fibroblasts, although this theory cannot be confirmed by the data presented in this study, and the histogenesis of GCF multinucleated cells remains unclear. In contrast, absence of either PCNA or Ki-67 immunoreactivity in PGCG multinucleated cells is consistent with an osteoclast lineage and formation from differentiated mononuclear cells.
|PCNA/cyclin expression and BrdU uptake define different subpopulations in different cell lines.
Coltrera, M D and Gown, A M
J. Histochem. Cytochem., 39: 23-30 (1991) 1991
Afficher le résumé
Monoclonal antibodies (MAb) to a 36 KD protein, proliferating cell nuclear antigen (PCNA/cyclin), have been previously shown to be capable of identifying proliferating cells in vitro as well as in alcohol-fixed, paraffin-embedded tissue specimens. The routine use of these anti-PCNA/cyclin MAb in investigative studies and in diagnostic pathology requires a clearer understanding of the distribution of PCNA/cyclin in the different cell populations found in tissue specimens. We therefore compared the ability of MAb to three nucleus-associated proliferation markers (MAb 19A2 to PCNA/cyclin; Ki-67 to an undefined proliferation-related marker; BU-1 to 5'-bromodeoxyuridine (BrdU) incorporated into DNA) to identify the proliferating cell fraction of various cells in vitro. The cell lines were chosen to represent a spectrum of proliferation rates (high to low) and cell lineage (mesenchymal vs epithelial, non-transformed vs malignant): (a) HeLa and A-431 (two malignant carcinoma cell lines with high proliferation rates); (b) SK-5 (a non-transformed fibroblast cell line with a low proliferation rate); (c) HUVE (a non-transformed human umbilical vein endothelial cell line with a low proliferation rate). Single and double labeling immunofluorescence studies were performed after uniform 1-hr incubations with BrdU. Comparison of the overlapping distributions of detectable PCNA/cyclin expression and BrdU incorporation demonstrated substantial qualitative and quantitative differences between the different cell lines. In two of the four cell lines (HeLa, A-431) the BrdU staining distributions formed inclusive subsets of the PCNA-positive cell populations. In the HUVE cell line the two populations overlapped incompletely. In one cell line, SK-5, the two populations were mutually exclusive. MAb Ki-67 demonstrated a pattern in the SK-5 cell line that was strongly predictive of PCNA positivity, while showing no associated patterns in the other three cell lines. We conclude that PCNA/cyclin expression detected by MAb may define different cell subpopulations in different cell types relative to those incorporating BrdU or expressing the target antigen for Ki-67. This has implications for the clinical study of mixed cell populations using these antibodies.
|Monoclonal antibody analysis of the proliferating cell nuclear antigen (PCNA). Structural conservation and the detection of a nucleolar form.
Waseem, N H and Lane, D P
J. Cell. Sci., 96 ( Pt 1): 121-9 (1990) 1990
Afficher le résumé
The proliferating cell nuclear antigen, PCNA, has recently been identified as the polymerase delta accessory protein. PCNA is essential for cellular DNA synthesis and is also required for the in vitro replication of simian virus 40 (SV40) DNA where it acts to coordinate leading and lagging strand synthesis at the replication fork. The cDNA for rat PCNA was cloned into a series of bacterial expression vectors and the resulting protein used to immunize mice. Eleven new monoclonal antibodies to PCNA have been isolated and characterized. Some of the antibodies recognize epitopes conserved from man to fission yeast. Immunocytochemical analysis of primate epithelial cell lines showed that the antibodies recognized antigenically distinct forms of PCNA and that these forms were localized to different compartments of the nucleus. One antibody reacted exclusively with PCNA in the nucleolus. These results suggest that the PCNA protein may fulfil several separate roles in the cell nucleus associated with changes in its antigenic structure.
|Monoclonal antibodies to proliferating cell nuclear antigen (PCNA)/cyclin as probes for proliferating cells by immunofluorescence microscopy and flow cytometry.
Kurki, P, et al.
J. Immunol. Methods, 109: 49-59 (1988) 1988
Afficher le résumé
Proliferating cell nuclear antigen (PCNA)/cyclin is an intranuclear polypeptide antigen that is found in both normal and transformed proliferating cells. We have recently described two mouse monoclonal antibodies reacting with PCNA. In this report we describe the application of these antibodies to the study of proliferating human cells by indirect immunofluorescence microscopy and by flow cytometry. A fixation/permeation procedure was developed in order to obtain satisfactory binding of monoclonal PCNA-specific antibodies to proliferating cells. This method involved fixation with 1% paraformaldehyde followed by methanol treatment. For the staining of cells in suspension with the IgM type monoclonal antibodies lysolecithin was added to the paraformaldehyde solution to achieve a better permeation by the antibody molecules. This procedure gave a good ratio of specific staining relative to the background staining. It also preserved the shape and normal architecture of the cells as judged by visual microscopic observation and by light scatter measurements using a flow cytometer. Furthermore, this fixation technique permits simultaneous labeling of DNA by propidium iodide and PCNA by monoclonal antibodies. PCNA was detected in various types of normal and transformed proliferating cells by indirect immunofluorescence. Quiescent peripheral blood mononuclear cells were PCNA-negative whereas a fraction of lectin-stimulated lymphocytes became PCNA-positive. Similarly, early passages of fetal skin fibroblasts were PCNA-positive but non-proliferating senescent fibroblasts of later passages were PCNA-negative. The association of PCNA-staining by monoclonal antibodies with cell proliferation was confirmed by flow cytometry. Simultaneous labeling of PCNA and DNA showed that the PCNA signal increased during the G1 phase of the cell cycle, reached its maximum in the S-phase, and declined during the G2/M phase. Using cell sorting we demonstrated that mitotic cells had a very low PCNA signal. Thus, monoclonal PCNA-specific antibodies offer a convenient tool for the detection of human cell proliferation by immunofluorescence microscopy and by flow cytometry.
|Monoclonal antibodies to a nuclear protein (PCNA/cyclin) associated with DNA replication.
Ogata, K, et al.
Exp. Cell Res., 168: 475-86 (1987) 1987
Afficher le résumé
Two hybridomas producing monoclonal antibodies to proliferating cell nuclear antigen. (PNCA)/cyclin were generated from spleen cells of BALB/c mice immunized with purified PCNA from rabbit thymus. The specificity of the monoclonal antibodies (19A2 and 19F4) was established by showing that they reacted in enzyme-linked immunosorbent assay (ELISA) with purified PCNA. Furthermore, they reacted in one-dimensional (ID) gel immunoblots with a 36 kD polypeptide which also reacted with human autoantibodies from lupus patients. Both monoclonals also recognized the nuclear polypeptide cyclin in two-dimensional (2D) gel immunoblots of HeLa cell proteins. Epitopes recognized by 19A2 and 19F4 were analysed by competitive inhibition test using a modified ELISA. The data suggested that the epitopes were closely related, but not identical. The data with human auto-antibodies were more difficult to interpret, although it suggested that some human anti-PCNA may share epitopes with 19A2 and 19F4, but in addition recognize different epitopes on the PCNA molecule.
|Cyclin/PCNA is the auxiliary protein of DNA polymerase-delta.
Bravo, R, et al.
Nature, 326: 515-7 (1987) 1987
Afficher le résumé
Identification of the cellular proteins whose expression is regulated during the cell cycle in normal cells is essential for understanding the mechanisms involved in the control of cell proliferation. A nuclear protein called cyclin of relative molecular mass 36,000 (Mr 36K), whose synthesis correlates with the proliferative state of the cell, has been identified in several cell types of human, mouse, hamster and avian origin. The rate of cyclin synthesis is very low in quiescent cells and increases several fold after serum stimulation shortly before DNA synthesis. Immunofluorescence and autoradiography studies have shown that the nuclear staining patterns of cyclin during S phase have a sequential order of appearance and a clear correlation can be found between DNA synthesis and cyclin positive nuclei. The proliferating cell nuclear antigen (PCNA) and cyclin have many common properties and it has been shown that these two are identical. Recently a protein which is required by DNA polymerase-delta for its catalytic activity with templates having low primer/template ratios has been isolated from calf thymus. We report here that cyclin and the auxiliary protein of DNA polymerase-delta are identical.
|Autoantibody to the proliferating cell nuclear antigen neutralizes the activity of the auxiliary protein for DNA polymerase delta.
Tan, C K, et al.
Nucleic Acids Res., 15: 9299-308 (1987) 1987
Afficher le résumé
Two murine monoclonal antibodies to the proliferating cell nuclear antigen (PCNA), a rabbit anti-N-terminal peptide antibody and human auto-antibody to PCNA reacted with the auxiliary protein for DNA polymerase delta from fetal calf thymus following SDS-polyacrylamide gel electrophoresis, confirming the identity of PCNA and the auxiliary protein. Undenatured auxiliary protein was immunoprecipitated by the human autoantibody, but not by the monoclonal antibodies, which were raised to SDS-denatured PCNA, nor by the anti-N-terminal peptide antibody, suggesting that the epitopes recognized by both the monoclonal antibodies and the anti-peptide antibody are not exposed in the native protein. The human anti-PCNA autoantibody neutralized the activity of the auxiliary protein for DNA polymerase delta, but did not inhibit the activity of pol delta itself. The ability of pol delta to utilize template/primers containing long stretches of single-stranded template was inhibited by the anti-PCNA autoantibody, whereas the activity of pol alpha on such templates was not affected, confirming the specificity of the auxiliary protein for pol delta. The ability of PCNA, a cell cycle-regulated protein, to regulate the activity of pol delta suggests a central role for pol delta in cellular DNA replication.
|Characterization of proliferating cell nuclear antigen recognized by autoantibodies in lupus sera.
Takasaki, Y, et al.
J. Exp. Med., 159: 981-92 (1984) 1984
Afficher le résumé
A nuclear antigen associated with cell proliferation (proliferating cell nuclear antigen [PCNA]) and blast transformation is recognized by autoantibodies in the sera of some patients with systemic lupus erythematosus. Using this autoantibody as a reagent, PCNA was purified 120-fold by ammonium sulfate fractionation, DEAE chromatography, and Sephadex G200 gel filtration. The antigenicity of PCNA was sensitive to trypsin but resistant to ribonuclease and deoxyribonuclease, suggesting that the antigenic determinant resided in protein and not nucleic acids. PCNA was inactivated at 56 degrees C for 30 min. Isoelectrophoretic focusing showed that the pI was 4.8. Analysis of immunoprecipitates on polyacrylamide gels showed the presence of IgG heavy and light chains and a single polypeptide band of 33,000 mol wt. This polypeptide band was the reactive antigen in immunoblotting (Western transfer) assays.
|Proliferating cell nuclear antigen in blast crisis cells of patients with chronic myeloid leukemia.
Takasaki, Y, et al.
J. Natl. Cancer Inst., 73: 655-61 (1984) 1984
Afficher le résumé
A nuclear antigen associated with cell proliferation [proliferating cell nuclear antigen (PCNA)] and blast transformation is recognized by autoantibodies in the sera of some patients with systemic lupus erythematosus; these autoantibodies are precipitating antibodies and also react in immunofluorescence, a technique that was used to determine if PCNA might be expressed in leukocytes of patients with chronic myeloid leukemia (CML) during certain phases of their disease. At all times, a strong relationship was seen between the percentage of cells stained by anti-PCNA antibody and the percentage of blast cells in peripheral blood leukocytes (r) = 0.865, P less than .001. However, during blast crisis, certain cells that morphologically looked like myelocytes, metamyelocytes, and some cells with segmented nuclei stained with anti-PCNA serum. This staining, which remained nuclear in location, was less intense than in blast cells, suggesting low density of the antigen in these nonblast cells. This phenomenon was not observed in myelocytes or metamyelocytes obtained from patients in remission. These initial studies demonstrated that anti-PCNA can be used as a reagent to detect blast cells in CML crisis and also has the capability to detect PCNA in other cells associated with blast crisis.
|Proliferating cell nuclear antigen (PCNA) and human malignant tumor nucleolar antigens (HMTNA) in nucleoli of human hematological malignancies.
Smetana, K, et al.
Blut, 46: 133-41 (1983) 1983
Afficher le résumé
Lymphoma (Lymphocytic non-Hodgkin's malignant lymphoma) and leukemic (chronic lymphocytic, acute and chronic myeloid, myelomonocytic leukemia) cells were studied by indirect immunofluorescence to evaluate the presence of proliferating cell nuclear antigen (PCNA) and human malignant tumor nuclear antigen (HMTNA) in their nucleoli. Most cells in lymph node smears of lymphocytic non-Hodgkin's malignant lymphoma (NHML) developed a bright nucleolar fluorescence with HMTNA antibodies. PCNA was detected in nucleoli of a limited number of cells which apparently represent the proliferating cell population in these lymphomas. Similarly, in the bone marrow smears of patients with chronic lymphocytic leukemia most cells possessed a nucleolar fluorescence for HMTNA and PCNA was present in nucleoli of a limited number of cells. In the bone marrow smears of patients with myeloid or myelomonocytic leukemias most blastic or monocytoid cells also developed a bright nucleolar fluorescence with HMTNA antibodies and PCNA was present only in a small percentage of these cells. Leukemic cells with PCNA in their nucleoli like thekhuntigen might represent a proliferating cell population in late G1-early S phase.
|Autoantibody to a nuclear antigen in proliferating cells.
Miyachi, K, et al.
J. Immunol., 121: 2228-34 (1978) 1978
Fiche technique
|Anti-PCNA, clone PC10 - Data Sheet


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Men's Soccer: Late goal lifts UCI past Tar Heels
Story | Nov 24, 2013 | 10:05 PM
Men's Soccer: Late goal lifts UCI past Tar Heels
Story | Nov 24, 2013 | 10:05 PM
College Roundup: UCI falls to Maryland
Story | Dec 3, 2013 | 11:52 PM
MEN'S SOCCER Maryland 1, UC Irvine 0 COLLEGE PARK, Md. — The visiting No. 12-seeded Anteaters matched the No. 5 Terrapins in shots, 10-10, but the hosts scored in the 32nd minute to end UCI's season in the NCAA round of 16 on Sunday. UCI (15-5-3)...
Next level features several locals
Story | Jan 26, 2012 | 11:01 PM
Corona del Mar and Newport Harbor are the top two teams in the CIF Southern Section Division I girls' water polo coaches' poll. Each has a top goalie to anchor their defense. The Sea Kings have senior Alex Musselman, a three-year starter. The Sailors...

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7 Strategies to Getting Unstuck with Elisa Balabram
Yesterday, I sat down with Elisa Balabram, author of "Ask Others, Trust Yourself: The Entrepreneurial Woman's Key to Success" to discuss her 7 strategies to getting unstuck.
(scroll down to watch the full webinar)
In the webinar, Elisa shared her strategies for moving forward with your business idea, creative project or other life goals.
Here are the 7 strategies that you'll learn more about in the call:
For the full strategies + bonus tips watch the video below!
Can't see this video? Click here to watch it on YouTube.
To connect with Elisa, visit www.AskOthersTrustYourself.com or email her at firstname.lastname@example.com.
You can also connect with Elisa on Twitter at @WomenAndBiz.
Join Elisa on Facebook for her 30-Day Self-Love Challenge, by clicking here.
Thank you to all the women who joined us on the call and added their questions to the conversation. I also want to thank Elisa for igniting the discussion with us!
Empowering Women to Achieve New Heights.


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Dancers, choreographers, bartenders, massage therapists, are more likely to divorce than vets, agricultural engineers and teachers, says a new research. Dr Michael Aamodt, an industrial psychologist at Radford University in Virginia, came up with the formula to work out the likelihood of success of a marriage based on the occupation of one of the partners.
Give your home a green makeover
Give your home a green makeover
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Here is the video for Niggas in Poorest by Yassin Bey F.K.A Mos Def. The video was directed by Yasiin Bey & Set Free, edited by Andre Cole and "produced by Yasiin Bey, Set Free and Andre Cole for Fellowship Mission".
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The Dewalt DW625EK is one of our best selling Router and is the ideal power tool for fitting Kitchen Worktops. The Dewalt DW625EK Router is available with or without a Dewalt Worktop Jig.
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In a recent interview, two investment gurus, Burton Malkiel and Charlie Ellis, shared three investing guidelines for those of us who are building or maintaining their retirement nest eggs.
One of the reasons that I also like these two giants is that they both are advocates, as I am, of indexed investing for those of us who are small-fry investors. And, if you are one who invests in a value stock and retains it for the long haul, you are a type of indexed investor, whether you call yourself that or not.
Both Malkiel and Ellis believe that the stock market will not be able to deliver future returns of the magnitude that it has in the past, since the risk free yields that are available in U.S. Treasuries are so low. They feel that a 6 percent return over the next 10 or so years is about what an investor should expect. With that scenario in focus, the costs of investing matter a lot – lower expenses are better. So, if you are investing in a mutual fund that takes 1.5 percent for management and other expenses, that charge represents 25 percent of your long-term return. As a result, no-load or low-load indexed funds may make sense.
Their second tip is to rebalance your investment portfolio regularly, by selling your appreciated stocks and buying back into lower cost stocks of well-run companies. In this way, one can reduce the temptation to take a flyer on the latest hot-tip from your brother-in-law.
I was interested to learn that these gents recommend that investors consider government debt from emerging markets, such as Brazil, where budgets and fiscal policies are relatively strong compared to many developed markets. The two also recommend that yield-seeking investors lighten up on bonds and generate income through shares of companies that are growing dividend payouts.
In that regard, here are nine attractive stocks for the income-conscious among us:
1. 3M Corporation which yields 2.4 percent and has strong financials.
2. Accenture yields 2.1 percent and is a worldwide consulting firm.
3. Automatic Data Processing yields 2.7 percent and is the king of payroll companies.
4. Coca-Cola yields 2.9 percent and has one of the most recognizable brands in the world.
5. Illinois Tool Works yields 2.4 percent and is a global industrial company.
6. Lancaster County yields 3.3 percent and makes condiments and lots of profit.
7. Sysco yields 3.3 percent and is one of the largest U.S. distributors of food.
8. Walgreens yields 2.6 percent and will soon be adding walk-in clinics.
9. WD-40 yields 2.3 percent and is a great buy.
The final tip from our two experts is that investing in indexed funds is the only way that the average investor can hope to keep up with the Wall Street big boys, who have better information, lower investment costs and superior experience. Ellis likened an investor who invests in individual stocks to someone who plays tennis every day against Serena and Venus Williams, since these two women tennis player represent the talent and skill levels of Wall Street's investment pros. Ellis said, "I don't think it makes any sense for individuals to take them on." I couldn't agree more.
Got a financial planning question for Greg? You may e-mail him at firstname.lastname@example.com
Greg Roberts is a certified financial planner with 35 years of financial and estate planning experience.
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The Armed Forces Health Professions Scholarship Program offers Medical School scholarships through the U.S. Army, Navy and Air Force. These one-, two-, three- and four-year scholarships cover all direct educational costs (i.e., tuition, fees, books, health insurance) and provide a monthly stipend for ten and one-half months per year. In return, there is a service commitment of one year of active duty service for each year of participation in the program, with a minimum commitment of three years.
For more information regarding the HPSP, please visit the following websites or contact OSVCP director Karen McNeil for recruiter information:


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|Main | About Us | Employment | Facilities | Memberships | Hours of Operation | Contact Us
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|Bike Share | Challenge Course | Educational Clinics | Gear Rental | Outdoor Trips | Project Marj
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Outdoor Adventures has all the outdoor equipment you need for your next outing, whether you are camping, backpacking, bouldering, hiking, or snowboarding. Check out our site or come in to the climbing gym located in Burdick Hall to talk to our equipment rental staff. See below for a complete list of equipment available for rental.
Not sure where to go for your next adventure? Our knowledgeable staff can assist you in planning and make recommendations for locations throughout the region for your next adventure.
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|Type of
|UAC code
|Pattern of
|Course start date
|Major offer round
B Aboriginal Professional Practice/B Laws (Honours)
B Arts/B Laws (Honours)
B Business/B Laws (Honours)
B Commerce/B Laws (Honours)
B Communication/B Laws (Honours)
B Science/B Laws (Honours)
B Social Science/B Laws (Honours)
|Newcastle – Callaghan
|23 Feb 2015
|21 Jan 2015
For information about when you'll need to apply to be considered in each offer round refer to UAC applications and offer round dates. Check the course search for the most recent final closing date.
Assumed knowledge: For Law: None. For non-Law degree: Refer to 482000 B Aboriginal Professional Practice, 482010 B Arts, 482200 B Business, 482300 B Commerce, 482350 B Communication, 484000 B Science or 484050 B Social Science.
Major studies: Law, plus majors from the relevant combination degree entries.
Practical experience: Students enrolled in the professional program undertake 180 hours of simulated exercises and placements with legal practitioners in each of their two final years.
Honours: For non-Law degree: May be available as an additional year (or part-time eqv) to meritorious students.
Professional recognition: This is an accredited law school for the purpose of admission to practise law in NSW.
Career opportunities: Examples include legal practice and employment in corporate administration, government departments, accountancy firms, merchant banking and management consultancy firms.
Additional information: Both degrees can be completed in five years of full-time study as the Law courses completed in the first three years count towards both degrees.
During the first three years, students complete four courses (the core program) and a designated number of courses for the second degree. The core program includes legal system and method, criminal law and procedure, torts and contracts. For the final two years of the program, only Law courses in either B Laws (LLB) or B Laws/DipLegPrac (the professional program) are undertaken.
The B Laws Program is designed for students wanting a general education in law. It has a limited clinical component. Students who complete this program must also complete postgraduate professional training approved by the Legal Profession Admission Board to meet requirements to practise as a lawyer.
Alternatively, the professional program merges traditional legal education with skills training and practical legal experience. Students who complete this program will receive a Dip Legal Practice as well as the B Laws degree. They will not have to complete postgraduate professional training or a period of practical legal experience before practising as a solicitor.
Admission to the professional program will be on a competitive basis and selection is based entirely on academic merit in the core program courses with at least three of the four courses being completed at Newcastle. Enrolment in the professional program is available on a full-time basis only.
Graduates wishing to study Law should refer to the Juris Doctor program www.gradschool.com.au
1. The B Commerce component does not include accountancy accreditation without additional courses being undertaken.
Course updates
Check UAC's course search throughout the 2014–15 admissions period for course updates, including new and cancelled courses.
Review of programs
The academic content of all programs is subject to routine review. For up-to-date details visit www.newcastle.edu.au/program
Applicants should note that under the revised Australian Qualifications Framework some programs with Honours are four year programs available with direct entry and other programs are three year end-on Honours programs that require a 3 year Bachelor degree for admission. Check the course descriptions carefully.
Key to abbreviations
For an explanation of the abbreviations used in this course description, visit Undergraduate course search results help


text: Meta Battle Subway PokeBase - Pokemon Q&A Login Register All Activity Questions Unanswered Tags Users Ask a Question Rules Chat Shedinja and Effort Value 2HP? 0 votes Shedinja has the worst HP in the game, How does beating him give 2 HP EVs? shedinja asked Apr 29, 2013 by Dinocharlie Please log in or register to add a comment. Please log in or register to answer this question. 1 Answer 1 vote What I guess is due to its ability which prevents moves from damaging him unless super effective. So making it seem like it had a lot of hp. And if that's not the case Thats how Gamefreak Wanted It answered Apr 29, 2013 by Đarkheart Please log in or register to add a comment. Related questions Ninjask and Shedinja; Double Ninja Shenanigans? Shedinja and Toxic Strategy? Will Poison Powder and Leech Seed hit Shedinja? What is the point in Shedinja having Defense and Special Defense stats? When evolving Nincada, will Shedinja and Ninjask both maintain Nincada's nature, IVs, EVs, and moveset? Questions about Shedinja and Spiritomb? Is Shedinja affected by Gastro Acid and Mold Breaker? If Shedinja had a Focus Band in battle, and it got hit, would it faint or survive? Useful Meta Posts How to change your username How to add a user avatar Why was my post hidden? The points system Question etiquette Flags vs Downvotes Anime FAQ


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Light-Filled Living Room
A cream-coloured palette allows pops of pattern to stand out.
"I love the blend of traditional and modern here, and the chocolate grasscloth on the ceiling makes for a dramatic statement," says Erin.
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It doesn't take a rocket scientist to know that in-flight entertainment and connectivity (IFEC) and social media go hand-in-hand. And indeed, we're already seeing evidence that major IFEC players Panasonic Avionics and Thales are bringing social media apps to their platforms. Panasonic's SeatTweet app, which I had a chance to try at AIX in Hamburg, is particularly clever. Here's the tweet I sent from a Panasonic IFEC system:
Airlines are wrapping their arms around social media and with good reason – it is now an essential marketing tool. It reaches loads of people (and indeed is becoming part of our daily lives), and, oh yeah, it's FREE (okay, you do need at least one warm body and ideally a social media strategy).
The carriers that understand how to harness the power of in-flight connectivity together with social media – I'm looking at you Lufthansa and your MySkyStatus – will be leaders in this industry.
So with all this attention being paid to the benefits of connected IFEC with social media apps, surely the major players in the field – Panasonic, Thales and other IFEC stakeholders – are participating, right? Wrong!
The Thales Group has a Twitter stream, but it pushes out tweets about everything going on within the group (space, defense, everything!) and really only uses the service as another public relations outlet…i.e. There is no back-and-forth conversation.
Sadly, Thales doesn't even own its own Twitter handle. Instead this person does. We call this a social media #Fail on Twitter. The Thales Group does have a FaceBook page, which is a little bit more dynamic but still very general.
Panasonic promises that it will be actively tweeting soon (see below), but again it doesn't appear to have a dedicated Twitter handle for IFEC. Another social media #Fail in my humble opinion. Its FaceBook page is also very general.
Both firms – and frankly all IFEC companies – should also be looking at building an IFEC presence on YouTube. Who better to showcase the latest and greatest IFEC systems coming to market – and the latest and greatest content – than the providers? Then we journos will take that info and push it to our growing networks. Yes, airlines brand your systems, but that doesn't mean you shouldn't be telling the world about what you do (hey, even Boeing is jumping into social media!)
Someone asked me today (on Twitter): "Will social media really help Panasonic or Thales win an RFP contract because they're more social?" Fair question. Here's my answer: "It can't freaking hurt!"
Indeed the World Airline Entertainment Association (WAEA) – which is rebranding as the Airline Passenger Experience Association (APEX) – is even starting to get more social, with the launch of the Passenger Choice Awards (it should be touting these awards on Twitter and FaceBook!)
Meanwhile, I can't wait for the day when I'll be able to tweet: Panasonic and Thales – IFEC social media #Success.
On an aside, I'm curious as to why the IFEC press hasn't jumped into the social media arena either. If you stay out of social media (or merely lurk in the shadows), you'll let others dominate a conversation that you're likely best equipped to lead (The same argument can be made for Thales and Panasonic and other IFEC players.)
Perhaps you think the real thought leaders in our industry aren't using Twitter. I can tell you that they are. Sure, some IFEC executives prefer to stay in the background, but these and others are becoming increasingly vocal.
Collectively, this industry has a tremendous opportunity to use social media to get the word out about how IFEC plays a crucial role in the passenger experience (and I assure that the passenger experience is one of the most popular topics on Twitter).
If nothing else, would you please jump on Twitter and help me dispel the myth that the iPad is going to kill embedded IFEC? My God, that could be a job in and of itself.
So, what do you say? Will you join me?
Here is what people are saying about the in-flight experience now.


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Full disclosure: Jennifer Gimenez from Celebrity Rehab is my girl. We spoke every week during this past season of the show and my interviews with her were incredibly insightful, sometimes emotional, and always fascinating, given her insider's point of view working at the Pasadena Recovery Center and with her own history of drug abuse. But often, our talks were sprinkled with girl talk and gossip, too, and Jenn occasionally mentioned this health regimen she had stuck to that sounded rough (no oil, no butter, no fun — that last one is just my own opinion). But giiiirl, it paid off. She lost over 100 pounds and we're stunned at the results. Check out Gimenez in this photo shoot from Star Magazine and tell us she doesn't look amazing. For more pics, check out RadarOnline, they have the full photo spread posted.
Jennifer Gimenez Flaunts Her Amazing Bikini Body After Losing 100 Pounds [RadarOnline]
[Photos: RadarOnline/Star Magazine]


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If you want to add a touch of class and sophistication to any setting, then you may want one.Tiffany table lamp are made with exquisite pieces of stained glass which are soldered together to form beautiful scenes or conceptual motifs. These beautiful lamps generally have a solid metal base which is also delicately decorated. The designer of these beautiful creations is none other than Louis Comfort Tiffany. The first Tiffany lamp to be made was in New York in 1899. He made it in his studio that…Read more…
Customer Retention - Listen to them
Today, is the day of the most digitally empowered customers! Your guests/customers don't like the experience at your restaurant, they will actively look out to voice their opinion. If the medium they choose to do so is on the internet forums (like Zomato, Yelp etc), then we have a problem. Negative word-of-mouth. This again leads to the dreadful phenomenon of Customer churn. Which means, you need to spent a lot more on advertising than to grow your customer base Automatically. So now what?…Read more…
THE NEXT STEP YOU TAKE By Vikash Mishra
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Is This Motivate You?
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What Most Of Foodies Choice? 1:- Taste 2:- Quantity 3:-Price 4:-QualityRead more…
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So, is generating sales via online food ordering more important to an operator than managing their reputation?
32 percent of U.S. teens named Instagram the most important social network...so what are restaurateurs doing about that?
The importance of customer feedbacks that will drive insightful actions and measurement of a restaurant performance is ever Increasing.
A startup called Afterall, has developed a product for just the same.
Voyce - www.myvoyce.in
Its really elegant and innovative with the design, while clearly intelligent to ask your customers the right set of questions.
Check them out, or drop a mail to email@example.com
We still believe the mobile and web food order sector is just beginning to affect change in the marketplace. New, fully-evolved business models track consumer behavior and it's all about delivery.
Seems that smarter restuarants win, when they know what their customers really want...basics like good food, good service and a clean, quality environment. Sounds good to us!
Internet commerce for restaurants will absolutely make a difference and help define your success in 2015


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Focus on MS Video Seminars
The Multiple Sclerosis Society of New Zealand
The Multiple Sclerosis Society of New Zealand (Inc) is a non-profit organisation formed to provide on-going support, education and advocacy for people with MS and their support networks. It also aims to educate the general public, employers and health professionals about MS and actively funds key research into the condition.
The Society was formed in 1967 and today there are 18 Regional Societies from Northland to Southland supported through advice, education and materials by the National Office.
Official launch of the New Zealand Multiple Sclerosis Research Trust
$900,000 in grants kick starts Trust dedicated to New Zealand-based Multiple Sclerosis research
The New Zealand Multiple Sclerosis Research Trust ("NZMSRT") today announces it has received a combined $900,000 investment portfolio as founding capital for the Trust. A $600,000 portfolio has been transferred from the Multiple Sclerosis Society of New Zealand and a $300,000 portfolio from the Multiple Sclerosis Auckland Region Trust. The Research Trust has been established to stimulate, co-ordinate and support New Zealand-based research into the cause, prevention, treatment, alleviation and cure of Multiple Sclerosis (MS), and to obtain and disseminate research findings.
Click Here to read more
The Multiple Sclerosis Society of New Zealand (MSNZ) is delighted by PHARMAC's announcement today that it will fund two new treatments for Multiple Sclerosis.
After years of funding submissions, culminating in a campaign this year led by MSNZ, PHARMAC has approved funding for two new treatments and increased access to those currently funded for New Zealander's diagnosed with relapsing remitting forms of Multiple Sclerosis.
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MS Regional Societies and Field Worker Service
If you would like further information and support you can contact your Regional Society directly. All our Regional Socieites employ skilled and experienced Field Workers who are available to you and your family and carers to provide on-going support, information and advice.
Contact your nearest regional MS Society
Home | About MS | Living with MS | Get Involved | News & Research |Contact Us Disclaimer | Privacy Policy2010 © Multiple Sclerosis Society of New Zealand


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Groups to 'shine light' on human trafficking Jan. 17 at mallWritten by Chase Will | | email@example.com
The third annual Shine a Light on Human Trafficking event will take place 1-5 p.m. Jan. 17 at the Franklin Park Mall.
During this free event, speakers from Northwest Ohio and Columbus will educate the public on what human trafficking is, how it occurs and how to prevent it.
"Two women who were actually trafficked are going to tell their stories," said Mary Jay, co-chairman of the Lucas County Human Trafficking Coalition, which is presenting the event along with Toledo Area Ministries and Second Chance.
There will be a presentation from 1-3 p.m. at Cinemark Theater and information available from 3-5 p.m. at a kiosk in the food court. Franklin Park Mall is located at 5001 Monroe St.
Among presenters will be Theresa Flores, a trafficking survivor who is now an author, victim's advocate and founder of Save Our Adolescents from Prostitution (SOAP); Jeff Willbarger, director of The Daughter Project; Sarah Ladd, attorney with Legal Aid of Western Ohio; and Michelle Moore, a trafficking survivor and support specialist/ chemical dependency counselor.
Toledo is the third largest origin city in the nation for human trafficking, and boys and girls as young as 8 are bought and sold daily, Jay said.
While the common misconception is these children are "snatched" off the street, Jay said traffickers typically finesse a child by using their personality vulnerabilities to gain their trust.
"They will target kids that maybe don't have high self-esteem, kids who are hungry for attention," Jay said. "Even when kids travel in groups, traffickers will go up and start talking to them and looking for the kid who seems to not think too highly of themselves."
Traffickers will typically prey upon children by offering to buy them name brand clothing or taking false interest in their lives in order to gain their complete trust.
This trust is built over time as the child hangs out with this "cool new friend," and the trafficker brainwashes the victim into trusting them completely, which leads to complete power over the victim's decision making and willingness to service clients.
As a health department employee, Jay meets daily with both victims and traffickers, typically for medical services such as STD testing.
"We had a trafficker and his victim here last November who were trying to get a birth certificate," Jay said.
"The trafficker will often want to have possession of the victim's identification. Think about it; it's pretty impossible to get service anywhere without a form of ID, and if trafficker withhold those things it enhances their level of control," Jay said.
Jay also said these arrangements don't always occur in the shadows or back alleys.
"Wherever you see a large influx of men who are unattached and not with their significant others, that's where you'll see a large demand for sex," said Jay. "We're talking car shows, the Super Bowl, all those big name sports events and conventions."
Jay said it's important to know human trafficking happens everywhere, not just in bigger cities like Toledo. Recent victims have been traced back to Fulton County and small towns across Ohio.
"The best thing parents can do is empower their kids and not have a child be so hungry for attention that they'll go against their own gut instincts," Jay said. "I would say for parents to empower their kids to not be so trusting of adults, to tell if something happens, such as if a stranger at the mall approaches and tells them they're beautiful and they should be a model."
Jay told a story about an incident which happened a few years back in Toledo. Two cousins, 14 and 15 years old, were kidnapped off the streets and taken across state lines after getting into an innocent-looking vehicle. One victim, who was later recovered, said every gut instinct told her not to get into the vehicle, but her trust in adults led her to disobey that instinct.
Even scarier, perhaps, is the large number of victims trafficked completely in the open on websites such as backpage.com, where traffickers use careful wording to sell victims the way an average Joe would sell items on eBay.
Jay said the FBI tracks websites known for promoting prostitution, and will set up reverse stings by luring offenders via fake postings.
"Toledo has one of the first human trafficking FBI divisions, if that gives you any idea how big it is here," said Celeste Rollins, co-organizer for the event. "We're known nationally as one of the hubs because of how our highways are set up. It's easy to access, and you can jump on I-75 and be in Indiana or Michigan in minutes."
Rollins got involved with the event after hearing a man speak on human trafficking during a church service. She had no idea at the time how prevalent it was, and with five children of her own, she was inspired to help raise awareness toward preventive actions.
Event attendees will leave with a wealth of knowledge as well as pamphlets detailing how to get involved in preventive organizations.
"One of the biggest things people can do is be aware and look for the signs," Rollins said. "That's what this event is all about."
Tags: Celeste Rollins, Franklin Park Mall, human trafficking, Lucas County Human Trafficking Coalition, Mary Jay, Shine a Light


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Google Earth for 557 boxing champs
Thursday, November 08, 2007
Recently, a group of Chicago Googlers who are passionate about sports came together to find ways to support Chicago's bid for the 2016 Summer Olympic Games. We got in touch with folks from World Sport Chicago, who had recently agreed to organize the AIBA World Boxing Championships, and asked them how Google technology could help support this local event with global reach. Together, we came up with an idea to educate fans about the boxers and their home countries through an information overlay on Google Earth, embedding select information about each boxer and their sponsoring country in pop-up windows across the globe.
Then we took this idea further by matching up our software with Accenture's Interactive Wall Technology to make the information even more lively. The Accenture Interactive Wall literally put the power of Google Earth at one's fingertips, so that people could manipulate the globe using their hands. Thousands of fans visited this display during the championships and we're pleased we could play a part in developing this learning tool for them.
When members of the Mongolian boxing team visited and asked how it worked, a volunteer helped them learn the controls, and they were quickly off to explore the planet. In a few seconds, they made their way to Mongolia, and were able to locate the gym back home where they had trained in for these very championships. They were amazed at the technology and the ease of use in finding their homes.
Chris Lobdell, one of our customer solutions engineers, worked closely with Bruno Bowden from the Google Earth team to build this interactive mashup. Using boxer profile information provided by the USOC, Chris merged the profile for each boxer and coach with data about the represented country (population, land mass and currency).
Googlers love a challenge, and this was an interesting one, especially considering the short timeframe in which it needed to be done, the massive information overlay development, and the integration with Accenture's display.


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Love is in the air at Harriet Kelsall Bespoke Jewellery in Cambridge
Posted: February 14, 2014|
As loved-up men (and women) up and down the country prepare to pop the question this Valentine's Day, LYDIA FALLON heads to Harriet Kelsall Bespoke Jewellery on Green Street to see the studio's dedicated team of ring designers in action, and have a play at commissioning a ring herself.
I'm a sucker for a love story; from Colin Firth's bumbling proposal to his Portuguese housekeeper in Love Actually to dishy Mr Darcy proclaiming his love ("You must allow me to tell you how ardently I admire and love you,") to Lizzy Bennet in Pride and Prejudice, I weep over every single one of them, and unashamedly so.
I dream one day my own Colin-Firth-alike will get down on one knee and produce a sparkling bespoke engagement ring, which he has designed himself (this is the point in the article when my boyfriend goes into hiding). I'll say yes, fall into his arms and then ride into the sunset on a fine stallion.
And then pigs will fly. I have a feeling I'm going to be waiting a rather long time for my Jane Austen-worthy proposal, but, in celebration of the most romantic day of the year, I thought I'd follow the lead of Cambridge's sweethearts and 'commission' my own bespoke ring anyway. A girl can dream, right?
I was booked in for a mock design consultation at Harriet Kelsall Bespoke Jewellery in Cambridge and, as a self-confessed magpie, it's safe to say I was just a little bit excited.
Launched in 1998, Harriet Kelsall Bespoke Jewellery is now one of the country's top bespoke jewellery design companies, has won numerous awards and is a mecca for couples looking to bring their love stories to life. Not bad for a business that in true Google Chrome style was started at Harriet Kelsall's kitchen table.
Nestled in Green Street, the studio glistens with swoonworthy gems, all immaculately displayed in pristine cabinets. I'm positively giddy as I whirl around in a daze, seeking out my favourite pieces.
Luckily my lovely designer April Page completely understands my obsession with things that sparkle.
"I've always been a magpie," she laughs, flashing an amazing engagement ring. "I'm obsessed with the art-deco style."
I eventually regain my composure and we settle down with a cuppa to begin the design process.
April explains she usually starts by trying to find out as much as she can about the customer and their other half. From their proposal stories to their personality, interests and style, anything she can draw on for inspiration when designing the ring.
"Some people even bring photos in and I've had storyboards before too," April laughs. "But I think the most important thing is that whatever we design is suitable for that person to wear every day. You don't want these magnificent show pieces that just sit in the drawer all the time."
The lead up to Valentine's Day is one of the busiest times of year for the jewellers, and April admits it is mostly, although not always, slightly sheepish men sitting in my position. "Some guys do get quite anxious about the process, and I guess it is overwhelming for them as it is quite a girly thing to be involved with, but I think it's lovely when a guy is confident enough to come in and do it," says April.
Customers are encouraged to get completely involved with the design of their ring, and the team have had some pretty wacky requests over the years.
"I think the strangest request I heard was a ring inspired by an octopus," April tells me. "At first we thought 'Is that a technical term?' but he literally wanted it to look like an octopus holding a diamond.
"Apparently they'd been on a holiday where they went scuba diving and coaxed out an octopus which was sparkly; so that was the reason."
Unfortunately I haven't given my perfect ring quite this amount of thought, but tell April that I do have a particular liking for aquamarine and sapphire gems (with a few diamonds thrown in for extra sparkle), and quite fancy the thought of a wave-inspired motif, having spied a gorgeous creation earlier.
April measures my ring finger ("We had one man who had to find out his girlfriend's size while she was asleep," April chuckles) and starts to gauge my ideal size and shape – do I want it to be all about the main stone? Would I want a twist style? Do I intend to match it to my wedding and eternity rings? – before leading me over to the cabinets to start trying some on.
I'd always thought I'd opt for a classic diamond ring (diamonds are a girl's best friend after all), but now feel swayed towards perhaps a more contemporary design with a blue stone.
What is the most popular choice the designers see? "Diamonds followed by sapphires," April says, showing me some of the favourite styles. "After Kate Middleton and Prince William got engaged we had a lot of people asking for a sapphire with diamonds around the outside just like Kate's ring."
As my own taste and style starts to become a little clearer, April grabs her sketchbook and begins to ink out three intricate designs. "We always draw to scale so people can visualise exactly what it will look like," April says.
I watch transfixed as the ring starts to take shape. It's in a classic twist style with the side open to reveal a beautiful aquamarine stone. Little diamonds sit either side, and I'm instantly smitten.
"Light blue shades look really pretty with white nine carat gold; it brings out the blue hue," April explains, as she brings out a variety of different bands and stones for me to muse over.
Looking completely contented as she sketches away, it's clear April adores what she does. "I obviously love working with the diamonds and looking at the different stones, but I really love listening to the different stories," she muses. "We had one guy who desperately wanted to give his girlfriend the ring on Christmas Eve, so he put it in her advent calendar. But she'd eaten loads that day, so said 'No, I don't want any more chocolate'. In the end he had to change it to New Year's Eve because she just wouldn't eat these chocolates."
As she adds the final touches to her sketches, April leans back to admire her handiwork. "What do you think?" she asks.
It's love at first sight; a contemporary but elegant design which captures everything I'd hoped for, as well as being a true one-off.
At this point, the design would usually be sent to the team of goldsmiths, and then the designers and goldsmiths work together in the same room to ensure every single detail of the piece is perfect. The experienced craftsmen use traditional hand-making skills, and April says seeing them in action is pretty "amazing" stuff.
Unfortunately my design will stay as that, just a design, but I do get to keep the sketches, just in case Cupid decides to strike this Valentine's Day after all.
For more information on Harriet Kelsall Bespoke Jewellery, visit the website at hkjewellery.co.uk.


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KINGSTON, R.I. -- April 8, 2002 -- The Center for Personal Financial Education has a new name, a new home, and a new grant.
Now called the University of Rhode Island Center for Personal Financial Education, the Center has moved from its first home in Warwick at the Consumer Credit Counseling Service of Southern New England to the Transition Center on URIs Kingston Campus. The $90,000 grant from the CDNE Foundation will help bolster its programs in workplace financial education, online credit education for college students, and outreach at schools.
The relocation of the Center and the awarding of the grant were announced at a recent ceremony on the Kingston campus. The event was attended by URI President Robert L. Carothers, URI Provost M. Beverly Swan, Stephen R. Bucci, president of Consumer Credit Counseling Service of Southern New England, Leonard OConnor, chief executive officer of AQM Solutions and chairman of the CDNE Foundation, in addition to staff members and supporters of the Center.
According to Swan, moving the Center to URI supports one of URIs main focus areasChildren, Families and Communities. "Since money matters are often at the core of a familys stability, we want to be at the forefront of providing information to Rhode Islanders that strengthen families and in turn the entire state."
Len OConnor, director of the CDNE Foundation, presented University officials with a check for $90,000 to support the Centers efforts. The CDNE Foundations mission is to support credit education in New England.
The mission of the Center for Personal Financial Education is to advance the adoption of sound personal financial practices by developing and delivering personal financial education programs and conducting related research. The goal of the Center is for all Rhode Islanders to attain financial well being as a result of informed decision-making about spending, saving, and investing their money.
Joan Gray Anderson, URI professor of Human Development and Family Studies, serves as the Centers director of research, and Claudia M. Kerbel is the Centers director of outreach.
"There is a huge need for this kind of information, particularly since the economy has been in a downturn," Kerbel said. "There are so many people who walk the thin line between financial control and financial crisis, and when that layoff notice comes or the overtime drops, they fall into deep financial trouble."
The Center, established in 1996, is a joint venture between URI and the Consumer Credit Counseling Service of Southern New England. Steve Bucci, who earned both his bachelors and masters degrees from URI, is currently the president of Consumer Credit Counseling Service and a director of the CDNE Foundation.
"Every day, we help people in financial trouble," Bucci said during the ceremony. "So its important that the URI Center has become a permanent part of the landscape. The Center is going to continue to help Rhode Islanders make better financial decisions."
The Centers main product is a program called Getting Fiscally Fit, a series of nine modules designed for financial educators. The units cover effective strategies for personal money management, credit, insurance, buying your first home, buying and leasing a car, investing, saving for college, retirement planning and financial matters during later life.
Each module contains a leader guide, computer-generated slide presentation, script, participant materials and instruments for a three-part evaluation. The program is distributed by downloading from the Centers website at www.gettingfiscallyfit.org or on CD. The cost is $295.
The Center also provides a free, semi-annual newsletter for 2,500 high school teachers around the region, which provides information about new research and teaching resources related to personal financial education.
In addition to Getting Fiscally Fit and the newsletter, the Center is currently conducting a study of the effectiveness of workplace financial education to measure changes in financial practices and improvements in productivity that result from financial education programming.
"We believe if people have their finances in order, they will be better, more productive workers. Theyll be making fewer phone calls to straighten out financial crises," said Anderson.
The Center also launched a new program last fall that was integrated into the sections of URI 101 offered to freshmen in the College of Business Administration. Kerbel and Anderson wanted to find out if financial education delivered using web technology would increase students knowledge about personal financial practices.
"The preliminary results show a real difference between those students who participated in the program and those in the control group," Kerbel said. Along with CCCS-SNE , the URI Department of Human Development and Family Studies and the Rhode Island Cooperative Extension sponsor the URI Center. In addition to the CDNE Foundation, other Center supporters include: the U.S. Department of Agriculture, URI Agricultural Experiment Station, and the National Endowment for Financial Education.
For a digital image of the ceremonies, please contact Dave Lavallee, URI Department of Communications.
For Information: Claudia Kerbel 401-874-743, Dave Lavallee 401-874-2116
Copyright © 1999
University of Rhode Island
For more information about this site, contact email@example.com
File last updated: Thursday, May 30, 2002
The University is an affirmative action/equal opportunity employer.
All rights reserved. URL: http://www.uri.edu/news/


text: Manning really has cemented his place among the all time greats. He kept his cool the entire game and shifted into a different gear in the 2nd half. They have a lot of weapons for Manning to play with, if Addai and Brown can eek out some yds on the ground they will be unstoppable.


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Palermo defender Carlos Labrin has confirmed he'll leave in January. "I have shown I am not ready."
The Chilean just turned 22 and has struggled to make a positive impact in Sicily since joining in January 2012.
"I am waiting for January to find another team that I can play with, because I want to play consistently," Labrin told Radio ADN.
"I have shown that I am not ready and perhaps this affected my experience at the club."
Meanwhile, Palermo defender Ezequiel Munoz has seen his one-match ban revoked after an error was spotted in the referee's report.
The official confirmed he intended to write down the name of teammate Edgar Barreto instead.
Think you know your Italian football? Share your knowledge, tips and comments to win cash prizes in OLBG's tipster competition [12] - £11,000 to be won monthly!


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With the release of the Samsung Galaxy S4 just a few months away the rumour mill has been heating up with leaks including case images and now new software features
The Samsung Galaxy S4 could feature a new camera mode called Samsung Orb which would allow users to stitch together a completely 360-degree image, similar to Google's own Photo Sphere feature found on Android Jelly Bean.
The leak comes from Android Geeks who claim to have been contacted by a software engineer working on the Galaxy SIV. The engineer claims that Samsung Orb will be better than Photo Sphere due to the Galaxy S4's rumoured 13MP camera.
Facebook integration has also been suggested with users able to upload their Samsung Orb pictures onto the social network allowing others to click on the image and then 'look around' inside the 360-degree photo.
The Galaxy S4 is yet to be confirmed however several companies have already started releasing videos of Galaxy S4 cases which have revealed that the smartphone will be around the same size as the Galaxy S3 but will sport a completely new design.
Other reports have claimed that the S4 will be Samsung's most powerful smartphone yet featuring a huge 1.8GHz eight-core chip called the Exynos 5 Octa chip.
While this directly contradicts SamMobile which believes Samsung's new superphone will come with a 2.0GHz quad-core processor, either would still make it the most powerful smartphone available trumping HTCs newly announced HTC One and wiping the floor of Apple's iPhone 5.
With Samsung set to unveil budget and mid-range smartphones such as the Galaxy Star at MWC 2013, users will have to wait until after the conference to find out what the Galaxy S4 will look like with rumours pointing to a mid-March release date.


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OM Puerto Viejo: Hostel & YogaCosta Rica, Limon, Puerto Viejo de Talamanca
Request to Book
About this listing
We are a budget hostel option located on the main surf break of the Caribe Sur. Minimal frills, but Yoga classes 2-4 times/day offered 7 days/week. This accommodation is most suitable for low-key yogis and surfers, backpackers, students and couples.
Space where guests can cook their own meals
Towels, bed sheets, soap, and toilet paper
In the building, free or for a fee
Space where guests can cook their own meals
Towels, bed sheets, soap, and toilet paper
Central heating or a heater in the listing
In the building, free or for a fee
In the building, free or for a fee
Continuous access in the listing
Breakfast is provided.
The listing can accommodate a gathering of 25 or more attendees
Easy access to the building and listing for guests in wheelchairs
Private or Shared
Free, in the building or nearby
Posted emergency information and resources
The Space
$35 USD/per night + 13% CR sales tax
This yoga studio / hostel offers ocean front location, discounts on yoga and surf classes, discounts on body and energy work and more!
The price includes:
-purified drinking water
-personal safe in each room
-shared fully equipped kitchen
-shared bathroom
-shared shower
-pay per weight laundry service
-regular cleaning (6x/week) and change of linens as needed
-Free WiFi with your personal device
-Free local phone calls
In addition find the CariBeans Chocolate Tasting Lounge in the front downstairs space - serving the best coffee, chocolate, ice creams and light lunches in the caribe! Everything you need all in one spot and on the most popular surf/sun bathing beach in the South Caribe. Yoga is offered 7 days a week and with your reservation of 5+ days you receive a complimentary yoga class.
OM is located a quick 5 minute bike ride from town center and a 10 minute bike ride to some of the most beautiful Souther beaches (PLaya Chiquita, Punta Uva). Far enough from the hustle and bustle, but close enough that you won't miss out on the action!
We offer a mix of backpacker's simplicity with spa elegance. Catering to the low-key yogi and the more mature globetrotter our space gives a feel of communal living with folks all over the world. Pura vida!
Guest Access
Immediate access to beach, yoga and surf classes, massages, energy work, chocolate tour, kayak tour, snorkelling, community kitchen, patio, beautiful gardens and more!
-No smoking on premise
-Deposits for reservations are non-refundable
-OM linens/towels may not be taken to the beach
-No overnight guests that are not registered/reserved guests of OM
-Clean and take care of your own dishes in the community kitchen
-Keep windows and doors locked when not on premise/in room
-Quiet hours from 10:30p.m. to 8a.m. daily
6 Reviews
Endroit parfait pour se relaxer et passer du bon temps ! Juste à côté d'un bar et à 1 minute de la plage
Pura vida !
Great location, clean hostel, really nice yoga space. The owner is on site often and is easy to communicate with. There is always a mgr available if needed! definitely a good experience especially the yoga.
Let me preface this by saying I've never been much of the earthy type...with that being said OM was an AMAZING place to ease into that laid back, backpacker vibe. Yes there are mosquito nets over the bed and yes the shower is outside (surrounded by metal for privacy of course) and yes there is a bar next door but that truly adds to the experience. If you're looking for a place to get a little closer to nature and step out of your resort/hotel comfort zone this is a great place to do it! The facilities were clean and while I felt like I was sleeping in the jungle I never felt overexposed and there were actually ALOT fewer bugs than I expected. Another added bonus is that since alot of the other people staying there were surfers, folks were in bed pretty early and it was generally quiet by 10pm or earlier. The cafe downstairs had some of the best chocolate, and the walk to town (Puerto Viejo) was pretty quick and painless with plenty of shops, restaurants, and friendly people. The beach is steps away and was surprisingly peaceful..no loud obnoxious people or music or naked folks but a great place to sun, swim or surf. As advertised this is not a resort or hotel so you shouldn't expect those types of accommodations or amenities but it is a great place and made my first few days as a first time solo traveler really comfortable and enjoyable. OM was without a doubt one of the main reasons I didn't wanna leave Puerto Viejo.
I had a very nice stay in the OM hostel. The people were very nice and helpful and it's nice to hang out in the little restaurant in the evenings and have a snack or a coffee in the afternoon. The yoga classes were great, with a nice view and with locals joining. The downside was a bit that it's directly at the sea, but separated by a street and the shower was cold ;)
Love this place!!! The rooms were clean and very comfortable. The yoga space is amazing! It overlooks the beach and the jungle - so neat! If you're thinking about a surfing lesson, Alex is an awesome instructor and a very kind guy. There's good energy here and Kristin makes everyone feel welcome.
The bathroom is clean and it provided its purpose. The downstairs common area is very neat, you meet different people who pass through their wonderful for coffee or chocolate tour.
The kitchen has all the necessary pots and pans for travelers who are on a budget. Drinking is provided for guest, so bring your refillable bottled water.
The only issue I see problematic for other travelers would be the establishment/bar next door. On Tuesday nights they have "club" night. The music is overly loud and obnoxious so for travelers staying there bring ear plugs. It's a shame the establishment next door can't be courteous and turn down the music.
Other than that, we would definitely come back to OM anytime of day! We miss you guys!
The hosts were very helpful and welcoming. Our room was very small and there was hardly any space to move. The location is very convenient. The Yoga class with Ro was amazing. The kitchen and bathroom could have been better kept. Great place to have breakfast on the premises.
About the Host, Om
HOSTEL Catering to low-key yogis and surfer backpackers.


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Politicians of both parties are shrieking about AIG's bonuses. The government can't do much about it because, of course, these are contractual obligations. (AIG could and should have gone to executives to renegotiate those contracts, but they couldn't unilaterally void the bonuses either.) But wait. This is precisely what flows from avoiding the bankruptcy courts. That is the legally proper and very efficient forum in which to void contracts, reduce liabilities, and throw out the otherwise entrenched and obviously defective management.
But in our bailout frenzy we are insisting that companies not utilize that time-honored mechanism. Instead we have contractual obligations we don't like, dense management, and taxpayer-sponsored inanity. It is the very worst of capitalism, in which bad behavior is not eliminated but subsidized by the government. As a conservative friend remarks, "This is incompetent crony capitalism." It's enough to make us all rabid populists.
So the next time Barney Frank or Larry Summers cry foul, the question should be: Why didn't the government send them to the bankruptcy courts? And better yet: Why is it still giving AIG and many similar firms billions of taxpayer money?


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Our Partners
The Ol Pejeta Conservancy's mission and programmes could not be realised without the help of our partners in Kenya and around the world. They help us mobilise resources, design strategies and implement programmes.
Highlighted here is a list of Ol Pejeta's major partners. We deeply appreciate their support: