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|By Daniel Pena | November 12, 2012 | Comments
The synopsis for the title reads: "In the late-90s, WWE was struggling for ratings survival while a cultural shift in the viewing audience's taste was occurring. "Saying your prayers and taking your vitamins" had lost its luster amongst the rebellious masses who preferred "flipping the bird" and "laying the smackdown" Enter Stone Cold Steve Austin, The Rock, Triple H and Mankind and the notorious crop of edge and fearless Superstars who ushered in The Attitude Era. Now in this 3DVD set fans can own all the infamous moments from this controversial and revolutionary era that redefined sports entertainment for an entire generation. Over 8-hours of envelope-pushing content includes all the agression, antics and innuendos that proved to be the perfect cocktail for an explosion of popularity."
Here is the listing, courtesy of Amazon.com.
The Birth of Attitude
Entrance Music
D-Generation X
Austin vs. McMahon
Long-Arching Stories
New Demographic
Wealth of Talent
The World Was Watching
Watershed Period
Jim Ross interviews Goldust & Marlena
Raw Nov 3, 1997
Steve Austin Throws the InterContinental Championship Off A Bridge
Raw Dec 15, 1997
Soldier of Love
Raw May 4, 1998
Mr McMahon Presents Mankind with the WWE Hardcore Championship
Raw Nov 2, 1998
Jim Ross Interviews Triple H
Sunday Night Heat July 25, 1999
An Evening At The Friendly Tap
SmackDown! Jan 20, 2000
Mae Young and the Acolyte Protection Agency
SmackDown! Jan 27, 2000
"The Jug Band"
Judgment Day 2000
Triple H Trains Trish Stratus
SmackDown! July 27, 2000
Edges Totally Awesome Birthday
Raw Oct 30, 2000
The Rocks Message to His Hell in a Cell Opponents
Raw Dec 4, 2000
Mike Tyson Joins DX
Raw Mar 2, 1998
A New Beginning For D-Generation X
Raw March 30, 1998
Sable vs. "Marvelous" Marc Mero
Raw May 11, 1998
Nation of Degeneration
Raw July 6, 1998
Brawl for All Match
Bart Gunn vs. "Dr Death" Steve Williams
Raw July 27, 1998
Four Corners Match for the WWE Tag Team Championship
The Undertaker & Stone Cold Steve Austin vs. Kane & Mankind vs. The New Age Outlaws vs. The Rock & Owen Hart
Raw Aug 10, 1998
Lions Den Match
Ken Shamrock vs. Owen Hart
SummerSlam 1998
Finals of WWE Championship Tournament
The Rock vs. Mankind
Survivor Series 1998
The Rock & The Undertaker vs. Mankind & Stone Cold Steve Austin
Raw Dec 7, 1998
Austin Gives The Corporation A Beer Bath
Raw March 22, 1999
The Undertaker vs. Stone Cold Steve Austin
Raw June 28, 1999
The Debut of Y2J
Raw Aug 9, 1999
Content still to be confirmed.
Special Features
(Expected Best Buy Exclusive)
GTV Al Snow & Head
Mae Young Gives Birth
Raw Feb 28, 2000
WWE Womens Championship Match
Lita vs. Stephanie McMahon-Helmsley
Raw June 12, 2000
The Dudley Boyz vs. The Hardy Boyz
Raw July 17, 2000
King of Kings Match
Ken Shamrock vs. Triple H vs. Owen Hart
Raw June 29, 1998
The Oddities w/ Insane Clown Posse vs. The Headbangers
Raw Sept 28, 1998
The Truth About Sammy
Raw Jan 18, 1999
The Unholy Union of Stephanie McMahon & The Undertaker
Raw April 26, 1999
The Rock vs. Val Venis
SmackDown Oct 7, 1999
Survivor Series Elimination Match
Stone Cold Steve Austin, The Rock, Kane & Shane McMahon vs. Triple H, X-Pac & The New Age Outlaws
SmackDown Nov 4, 1999
WWE Hardcore Championship Match
Al Snow vs. Crash Holly
SmackDown June 29, 2000
The Hardy Boyz & Lita vs. Perry Saturn, Eddie Guerrero & Dean Malenko
SmackDown Nov 30, 2000
Chris Jericho & The Dudley Boyz vs. Kurt Angle, Edge & Christian
Raw Dec 25, 2000
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John Cena Injury Update From Last Night's RAW
** SPOILERS ** TNA Impact Wrestling Tapings From 7/27
Photo: John Cena Suffers Broken Nose On RAW
Security Asks Fans To Stop Streaming At RAW, Finn Balor Milestone, Becky Lynch On WWE SmackDown
More Special Guests For This Week's WWE Tough Enough, Lita Wears A GoPro In The Ring (Video)
Ronda Rousey UFC Embedded Episode, UFC Rankings Movement, UFC 190 Countdown (Video)
Brock Lesnar - Paul Heyman Update, WWE 2K16 Commentary Team, Kevin Owens, Paige - Steve Austin
WWE Star Fuels Rumors On A Possible Gimmick Change
Big Update On The Rumors Of WWE SummerSlam Being A Four Hour Pay-Per-View This Year
WWE Monday Night RAW Results: John Cena Defends, Divas Matches, Wyatts, More
Rey Mysterio On How He Left WWE, If Door Is Open To Go Back, Never Winning WWE IC Title, More
Brock Lesnar - Undertaker Note, When Stephen Amell May Confront Stardust On RAW, David Otunga On TV


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ACE Fit Share
March 19, 2013
Pediatricians and other health professionals encourage mothers to breastfeed due to the considerable amount of research suggesting it benefits the health and well-being of both mother and baby. The evidence is compelling that breastfeeding helps moms to increase bonding with baby and decreases the mother's risk of developing rheumatoid arthritis, cardiovascular disease, and breast and ovarian cancer. (Whether breastfeeding increases postpartum weight loss is uncertain.) Furthermore, breastfed babies have a lower risk of respiratory, ear and gastrointestinal infections; sudden infant death syndrome (SIDS); and allergic (asthma, eczema) and immunologic disease (celiac disease and type 1 diabetes)
It has been widely reported that breastfeeding also decreases the risk of childhood obesity. However, a few recent studies have cast doubt on that belief. First, a study published in The New England Journal of Medicine labeled this belief a myth and pointed out that the studies supporting the weight-control benefit of breastfeeding were extremely biased. Now, a study just published in the Journal of the American Medical Association says the same: Breastfeeding does not prevent childhood obesity.
The Scoop
For this study, researchers recruited 17,046 breastfeeding mother-infant pairs in Belarus. Half of the participants received intensive coaching to exclusively breastfeed based on Baby Friendly Hospital recommendations. The other half received "usual practice," with no additional breastfeeding advice beyond what the hospital normally provides. The researchers found that breastfeeding duration and exclusivity increased in the intervention group (43% versus 6% exclusively breastfed at three months and 7.9% versus 0.6% at six months). Nearly 12 years later, the researchers followed up by measuring the children's body mass index (BMI), waist circumference, skinfold thickness (a measure of body fat) and levels of the hormone insulin-like growth factor 1 (IGF-1), a marker of body fatness. The outcomes were no different between the two groups.
What ACE Thinks
This study provides good evidence that breastfeeding in and of itself may not reduce risk of childhood obesity. However, the large number of other research-supported benefits of breastfeeding are compelling enough to continue to encourage and support moms in breastfeeding their babies. Plus, while breastfeeding itself may not protect against childhood obesity, it does offer a model of some important feeding strategies to help kids grow to be overall healthier (and less picky) eaters. For example, breastfed babies exposed to a wide variety of flavors from mom's breast milk are more likely to be willing to eat those foods later on. Breastfed babies are also better at regulating intake—not so much because of the milk itself, but mostly because the baby is in total control of how much he or she eats. Bottle-fed babies (whether they are fed formula or breast milk) are more likely to eat based on the number of ounces provided in the bottle.
What Does This Mean For Me?
These research findings won't change the general recommendation to breastfeed for at least one year, ideally with six months of exclusive breast milk, for optimal health benefits for mom and baby. While the list of benefits of breastfeeding may be slightly shorter, with overweight and obesity no longer included, numerous other benefits continue to make breastfeeding the preferred choice for babies.
Here are some important tips for parents who are committed to setting the stage for their kids to grow to be healthy eaters and maintain a healthy weight:
Natalie Digate Muth, MD, MPH, RD, FAAP is the Senior Advisor for Healthcare Solutions for the American Council on Exercise, a board-certified pediatrician and Fellow of the American Academy of Pediatrics, a Diplomat of the American Board of Obesity Medicine, registered dietitian and board-certified specialist in sports dietetics, and ACE Certified Health Coach. She is the author of "Eat Your Vegetables and Other Mistakes Parents Make: Redefining How to Raise Healthy Eaters" and the textbook "Sports Nutrition for Health Professionals." She has been ACE certified since 1998.
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Lyrics Depot is your source of lyrics to When the Shit Hits the Fan by Circle Jerks. Please check back for more Circle Jerks lyrics.
Artist: Circle Jerks
Album: Golden Shower of Hits
in a sluggish economy
hits the land of the free
standing in unemployment lines
blame the government for hard time
we just get by
however we can
we all gotta duck
when the shit hits the fan
10 kids in a cadillac
stand in lines for welfare checks
let's all leach off the state
gee!the money's really great!
soup lines
free loaves of bread
5lb blocks of cheese
bags of groceries
social security
has run out on you and me
we do whatever we can
gotta duck when the shit hits the fan
it's punk rock
Circle Jerks Lyrics
Circle Jerks Golden Shower of Hits Lyrics
More Circle Jerks Music Lyrics:
Circle Jerks - American Way Lyrics
Circle Jerks - Don't Care Lyrics
Circle Jerks - Fortunate Son Lyrics
Circle Jerks - Golden Shower of Hits (Jerks on 45) Lyrics
Circle Jerks - I'm Alive Lyrics
Circle Jerks - Operation Lyrics
Circle Jerks - Sinking Ship Lyrics
Circle Jerks - What's Your Problem? Lyrics


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Mighty Beau, who just missed last time out in the Shakertown Stakes (gr. IIIT) at Keeneland, easily took the $113,500 Aegon Turf Sprint (gr. IIIT) at Churchill Downs Friday.The five-furlong grass sprint is usually known for a late charge down the stretch. But Mighty Beau and Pat Valenzuela saw to it that didn't happen this year. Always in a good spot - second or third - the pair burst to the lead and made it no contest down the stretch. The winning margin was 2 ¾ lengths.Mighty Beau, by Rainbow Prospect, was winning his ninth race in 37 starts. He is owned by Mike Cloonan and Anthony Carolan and trained by Jeff Mullins. The 6-year-old bay gelding has two wins and three narrow second place finishes in five starts in 2005.Longshot Chosen Chief, Robby Albarado aboard, had aim on the winner in the stretch but settled for second best. Sgt. Bert, who was near the early pace, held on for third.The final time was :56.18 after fractions of :21.66 and :44.16.Mighty Beau, carrying 121 pounds, paid $5.80, $4, and $3.20, and Chosen Chief returned $11.60 and $6.80. Sgt. Bert, who was 1 ¼ lengths back in third, was worth $8.60 to show.The Sprint winner, bred in Oklahoma by Mighty Acres, earned $70,370, swelling his career bankroll to $510,991.The 123-pound high weight Wrzeszcz finished fourth. Parker Run, De Valmont, Deputy Storm, Elegant Fame, Testify and Montstar completed the order. Wildcat Shoes scratched.(Chart, Equibase)
Mighty Beau Shows Muscle in Turf Sprint
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Chargers WR Malcom Floyd plans to retire after 2015 seasonWednesday, June 10, 2015, 12:16pm
A proper farewell tour for Malcom Floyd can now begin.
The Chargers wide receiver reaffirmed Tuesday he will retire after the 2015 season. This long has been the plan for Floyd, who will turn 34 in September and is entering the final year of his contract.
Stevie Johnson's arrival may put a dent in Floyd's targets (92 in 2014), but Johnson was brought in mainly to replace the departed Eddie Royal (91 targets), so Floyd should continue to post solid numbers. He was the 72nd receiver drafted last season and finished the season in the #40 spot. Don't be surprised if he repeats those numbers, though due to his somewhat spotty big play tendencies, he's better suited for best ball formats than standard leagues.
Chargers Beat: Philip Rivers, Danny Woodhead have 'a special rapport'Tuesday, June 9, 2015, 1:03pm
When Chargers RB Danny Woodhead broke his ankle, quarterback Philip Rivers praised the replacements, but said no one player could match Woodhead's knack for winning on option routes, clever pass plays in which Rivers anticipates whether Woodhead will go north, east or west depending on the defense. It would take time, Rivers said, because he and Woodhead had developed a special rapport.
Assuming he's fully healed, he should resume his role as the Chargers' passing down specialist. In 21 games with the Chargers (including the playoffs), Woodhead has averaged 11.0 touches (4.1 catches) for 58 yards and 0.43 touchdowns, which equate to #13 RB numbers in PPR formats (#21 in standard). He's not likely to post those numbers with Melvin Gordon coming aboard, but he should continue to see a significant amount of touches as the team's primary third-down and no-huddle back, making him an excellent value late in drafts.
Ravens Beat: WR Kamar Aiken 'stole the show' during second OTA practiceTuesday, June 9, 2015, 12:50pm
Ravens WR Kamar Aiken quite simply just gets the job done, and he does it in a variety of ways.
He stole the show last week during the Ravens' second OTA practice open to the media. Aiken hauled in a deep bomb, stretched for a fingertips grab over the middle, tiptoed the sideline and out leapt a defender for a score.
Aiken was a jack-of-all-trades last season too in a breakout campaign. He caught 24 passes for 267 yards and three touchdowns during the regular season. He scored a 19-yard touchdown against the New England Patriots in the divisional playoffs.
With Torrey Smith in San Francisco and Jacoby Jones in San Diego, Aiken now has a chance to become a starting wide receiver. There's a lot of competition for the spot, but Aiken is competing on the first team and looks to have just as much a chance as any other.
Aiken was 12th in the league in fantasy points (PPR) per target last season. Breshad Perriman figures to win the starting job opposite Steve L Smith, but Aiken could serve as the team's WR3 or even start if Perriman struggles out of the gate.
Chargers Beat: RB Danny Woodhead 'back to form'Tuesday, June 9, 2015, 1:02am
Chargers HC Mike McCoy on Danny Woodhead (fibula): "He looks the way (he did) the snap before ... he went down" versus Buffalo in Week 3. Back to form.
Woodhead is signed through the 2016 season, so assuming he's fully healed, he should resume his role as the Chargers' passing down specialist. In 21 games with the Chargers (including the playoffs), Woodhead has averaged 11.0 touches (4.1 catches) for 58 yards and 0.43 touchdowns, which equate to #13 RB numbers in PPR formats (#21 in standard). He's not likely to post those numbers with Melvin Gordon coming aboard, but he should continue to see a significant amount of touches as the team's primary third-down and no-huddle back, making him an excellent value late in drafts.
Rams receivers 'love' QB Nick Foles and his 'great deep ball'Friday, June 5, 2015, 4:50pm
Quarterback Nick Foles is getting to know his new St. Louis Rams teammates.
"We love him," wide receiver Kenny Britt said Thursday after the second OTA session and first open to reporters. "Great deep ball, he puts it on the money every time.
"He can throw every pass in the book."
"He's got his hands full because we've got great defensive team speed and they're doing a lot of things over there right now," HC Jeff Fisher said. "We've just really been pleased."
Foles' second season as the starter in Philadelphia didn't go as well as his first. After a ridiculous 27-to-2 touchdown-to-interception ratio in 2013, Foles tossed 13 touchdowns against 10 picks last year. It wasn't all his fault, however, as he was playing behind a patchwork offensive line and didn't seem as comfortable in the pocket. In the seven games that he finished in 2014, he averaged 16.9 fantasy points, which is about what Philip Rivers scored as the #12 QB on the season. Foles ultimately broke his collarbone in Week 9 and missed the final eight games of the season. Now he joins a run-oriented Rams' offense that lacks consistent playmakers in the passing game. Sam Bradford had a few good stretches while in St. Louis, so there's no reason that Foles can't post QB2-type numbers if things break his way.
49ers QB Colin Kaepernick sporting a 'more compact' throwing motionFriday, June 5, 2015, 1:28pm
If you think 49ers QB Colin Kaepernick's release is more compact, you're right.
"It's the first thing everyone sees -- how fast the ball comes out of his hand," QB Dennis Gile said. That's a result of all the alterations -- from his head to the bottom of his cleats -- that the quarterback has made. "It's not about how hard you can throw it or how fast you can get rid of it," Gile said. "It's how quickly he can release it while still being relaxed."
One of the main things that changed was Kaepernick's stance. Gile said it was too narrow. That is, his feet were close together -- "real tall on his tippy toes," Gile said -- and when he took a step to throw the ball, that step was too big, too exaggerated. That elongated his release.
It's also helped his accuracy. Gile said Kaepernick made the biggest gains with his deep passes. That has to be good news for wideouts like Torrey Smith and Jerome Simpson, who were brought in this offseason to add firepower to a deep passing attack that had been lightly utilized in previous seasons.
Gile said he expected Kaepernick to return to Phoenix and continue to work on his craft during the team's break in early July.
It was reported earlier in the offseason that the team planned to utilize Kaepernick's running ability to a greater degree. Kaepernick ran the ball 12 more times in 2014 than he did in 2013 for an additional 115 yards, so it's not like he abandoned the scramble. It sounds like it's more about utilizing the read-option as a larger part of the offense. If that's the case, it could be a boon to Kaepernick's fantasy value. He was the #14 QB in 2014 after finishing #9 the season before, though his overall production only dipped by 11 fantasy points. He's a bounce-back candidate provided new OC Geep Chryst can design an offense that can move the ball. Chryst previously served as the OC for the Chargers in 1999 and 2000, but his offenses ranked 26th and 28th overall in that span. Any gains that Kaepernick can make as a thrower (due to his offseason work with Gile and former Rams/Cardinals QB Kurt Warner) will only help his overall value.
Cardinals say RB David Johnson will be a 'big piece of the puzzle'Thursday, May 28, 2015, 3:22pm
David Johnson, a third-round pick out of Northern Iowa, ranked 15th in FCS in 2014, posting 1,553 rushing yards while finding the end zone 17 times. He is expected to make a big contribution to the Cardinals offense this season, according to his position coach, Stump Mitchell.
"He's a guy who has a lot of confidence in his ability to play," Mitchell told Doug and Wolf Thursday on Arizona Sports 98.7 FM. "He's coachable. He's a smart young man, he's physical. He's going to make Andre (Ellington) pick up his game when given the opportunity.
"He has excellent hands and he's a bigger target to throw the ball to. He's going to be a big piece of the puzzle before this season is said and done."
Mitchell gushed about Johnson's physicality, citing his ability to run over people as well as run around them. He also conjured a comparison to one of the NFL's most productive backs over the last five years.
"He reminds me a lot of (Chicago RB) Matt Forte," Mitchell said. "You can put him outside and use him as a receiver and he's a good runner."
The Cardinals have been trying to add a bigger back to complement Andre Ellington, and Johnson fits the bill. He does a lot of the same things as Ellington, so for now we're expecting that he'll serve in a backup capacity, but Mitchell sounds as if Johnson will play a larger role than a typical backup. We currently project him for around 147 touches.
Cardinals RB Andre Ellington 'not limited in anything...feeling good' in OTAsFriday, May 22, 2015, 1:15pm
On the field for the first day of OTAs (organized team activities) Tuesday, Andre Ellington proclaimed himself healthy and ready to go.
"I'm not limited in anything," he said. "I'm out here running, running around, feeling good."
None of Ellington's injuries were the type that would keep him out long-term or inhibit him going forward. Once healed, he would essentially be the same player he was prior to getting hurt.
The Cardinals drafted David Johnson to lighten Ellington's workload, and a reduction in touches may actually help his effectiveness, though it's unlikely to help his per game fantasy production. Ellington averaged 22.0 touches per game, but only managed 3.3 YPC after averaging 5.5 YPC in his rookie season.
Chargers RB Melvin Gordon already turning headsFriday, May 15, 2015, 4:02pm
Via Michael Gehlken of U-T San Diego, the Chargers RB Melvin Gordon is already making an impression a day after signing his contract, even though teams aren't able to do much resembling football at the moment.
"He did a nice job," Chargers head coach Mike McCoy said. "We weren't really going full speed out there, just really an introduction to make sure everyone's got a hat on hat and guys are running to the right spot.
"But he makes a few of those moves and cuts, you understand why we took him where we did."
The Chargers traded up to get Gordon, so we'd expect they'll use him early and often in 2015. In fact, GM Tom Telesco used the phrase "impact player" 11 times in Gordon's introductory press conference. Ryan Mathews is gone, so it's Gordon's job to lose. He doesn't have a lot of experience as a receiver, so we'd expect that Danny Woodhead still gets a lot of work on third down while Gordon establishes himself as a good weapon out of the backfield. Given Todd Gurley's ACL recovery, we'd also expect that Gordon will be the first rookie running back off the board.
Monday, May 11, 2015, 9:32am
WR DeAndre Carter is only 5 feet 8 and 185 pounds, but he was one of the quickest and most active players on the field in the portion of Ravens' mincamp open to reporters on Saturday.
Carter was a coveted college free agent, but he looks up to Steve Smith, so signing with the Ravens made sense. He also had an extremely prolific college career, albeit against lesser competition. Carter caught 99 passes for 1,321 yards and 17 touchdowns last season and has return skills that could come into play with the Ravens looking to replace Jacoby Jones.
The article goes on to say that the Ravens' receiving lineup will be tough to crack, but the team is generally hurting for playmakers at the position. If Carter displays a skill set that the team needs, then he'll make the cut.
Cardinals RB Andre Ellington (foot) working his way backSunday, May 10, 2015, 1:00pm
Cardinals RB Andre Ellington on his recovery from a foot injury: "I'm close to doing a lot of things I've done in the past. Just (this week) I was able to do all my cuts and put a lot more pressure on my foot."
Ellington felt the opposite of threatened when the Cardinals drafted running back David Johnson in the third round last week. If Johnson, from Northern Iowa, is what the Cardinals think he is, he will lighten Ellington's load.
"I don't see why not," Ellington said when asked if he would be helped by the addition of Johnson. "It allows me to have more rest time, and it should keep the offense upbeat."
The Cardinals drafted Johnson to lighten Ellington's workload, and a reduction in touches may actually help his effectiveness, though it's unlikely to help his per game fantasy production. Ellington averaged 22.0 touches per game, but only managed 3.3 YPC after averaging 5.5 YPC in his rookie season.
Oddsmakers say Chargers RB Melvin Gordon is the favorite to win OROYTuesday, May 5, 2015, 7:06pm
Las Vegas oddsmakers have made running back Melvin Gordon the favorite to with the AP Offensive Rookie of the Year.
The sportsbook Bovada.com lists Gordon's odds at 13 to 2.
Quarterbacks Jameis Winston and Marcus Mariota, drafted first and second, are next at 7 to 1.
Behind them are three other players drafted before Gordon: receiver Amari Cooper 15-2, running back Todd Gurley 8-1 and receiver Kevin White 10-1.
All six players were first round picks. We agree that Gordon has the best chance to shine among the running backs, especially since Gurley is coming off of an ACL tear.
Cardinals Beat: David Johnson 'unlikely' to supplant Andre EllingtonMonday, May 4, 2015, 12:02am
Cardinals RB David Johnson has the makings of a player who can have an immediate impact out of a small school. He'll enter offseason workouts with an opportunity to be a three-down running back if he can learn the Cardinals' offensive scheme, coach Bruce Arians said.
It's unlikely, however, that he'll supplant Andre Ellington as the starting running back.
"I think Andre will continue his same role," Arians said. "We'll keep him healthy and let him continue to develop as a player, but the nice thing David can do is he can do everything Andre does, so you don't have to change if there was an injury."
The Cardinals have been trying to add a bigger back to complement Ellington, but it sounds as if Arians feels Johnson is more of an Ellington clone. Arians confirmed that Ellington should continue in his current role with Johnson serving as his primary backup.
Cardinals Beat: RB David Johnson 'will contend' for RB1 jobSaturday, May 2, 2015, 10:45am
Coach Bruce Arians doesn't like the word "need", but the Cardinals were lacking a big, physical back. David Johnson will contend for the starting job, and at least should be a nice complement to Andre Ellington. Johnson is big and he ran the 40-yard dash at the combine in 4.5 seconds, which is plenty fast for a man his size.
The Cardinals have been trying to add a bigger back to complement Ellington, and Johnson fits the bill. It's starting to look like a committee in Arizona, so keep an eye on this camp battle to see if Johnson's able to unseat Ellington. For now, Ellington should be able to hold onto the job with Johnson serving in a change-of-pace role.
Chargers prioritize the running back position by drafting Melvin GordonSaturday, May 2, 2015, 10:27am
Chargers GM Tom Telesco on the running back position in general (and Melvin Gordon in particular):
"The really, really talented ones are going to get drafted high. A top back is going to touch the ball 15 to 20 times a game, at least, a top receiver maybe eight or nine. You've got to have one.
"And I've always prescribed to the theory that you've got to have more than one of them. Melvin gives us that dynamic -- he can make the big play (which Mathews, whose longest run from scrimmage was 51 yards, his second-longest 39, could not). We've been a long-drive offense."
The Chargers traded up to get Gordon, so we'd expect they'll use him early and often in 2015. In fact, Telesco used the phrase "impact player" 11 times in Gordon's introductory press conference. Ryan Mathews is gone, so it's Gordon's job to lose. He doesn't have a lot of experience as a receiver, so we'd expect that Danny Woodhead still gets a lot of work on third down while Gordon establishes himself as a good weapon out of the backfield. Given Todd Gurley's ACL recovery, we'd also expect that Gordon will be the first rookie running back off the board (in the 3rd round?).
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If you have multiple kiddos sharing one bathroom, you know a towel organization system is a must. Rebecca from Rebecca's Round Up was inspired to create monogrammed towels for each of her three children. Rebecca knew a clean, straight line of monogrammed towels would eliminate any debates over whose towel is whose and where they belong. Best […Read More]
Kid's Monogrammed Bath Towels
Patriotic Burlap Stars
A few days ago I shared patriotic burlap stars for the Fourth of July. If you need one more nudge to break out the burlap and paint, then these beauties from The Kim Six Fix should do the trick! As soon as Kim saw Pottery Barn's stars, she knew they'd be easily to replicate and […Read More]
Trunk-Style Nightstand Makeover
Oh, how I love a good before and after, especially when it looks as amazing as this nightstand makeover from Jen at Girl in the Garage. Jen started out with two beat-up (as in missing a chunk of wood and covered in scribbles) campaign style nightstands. However, she worked some serious DIY magic to make […Read More]
A Trio of Driftwood Coastal Signs
Jen from City Farmhouse was inspired by beach signs from Pottery Barn to make her own rendition using driftwood she collected following Hurricane Sandy. Jen used a simple chalk technique to transfer and hand paint the lettering onto her wood. Living in New England, the signs are the perfect way for Sandy to easily incorporate some personal […Read More]
Burlap American Flag Pillow
Debbie from Confessions of a Plate Addict was shopping at Wal-mart last summer when she came across burlap sold in two patriotic patterns. She knew exactly what she was going to do – make a Pottery Barn knock off pillow for the Fourth of July! This was a pretty simple DIY. Debbie just sewed together […Read More]
DIY Bookshelves for Office Storage
Anne from Anne with an E was inspired by Ana White and the ladies over at Shanty 2 Chic to build two new bookcases to flank her vintage library desk. She knew she could really use the storage but sure didn't want to spend $1,800 at Pottery Barn for two shelves in a basement office. […Read More]
Painted Faux Coral for Summer
Although we have been enjoying warm temps and plenty of pool time for awhile now, it's actually only a couple of days into the official summer season. That means those of you who haven't even thought about summer decorating still have plenty of time, and Shannon from AKA Design is here to make it EASY […Read More]


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Ivan, thank you very much! this photo is one of ther fisrt and it was great start for my photo discoveries till now!
Greeting from Kharkiv, Ukraine!
Hm.. I think it is very interesting and unusual! Impressive constructivism beauty!
Výborný záber LIKE + FAV.
Zdravím Jaro.
Very nice ...Greetings azimli .L.
YSL 20/56; A very beautiful panorama! Peter, I have been there, at Neiden, Kirkenes and other places! You refreshed my mind! All the best from Norway!
Beautiful presentation of the church, LIKE + F, greetings Agathe
Very nice church, LIKE + F, greetings Agathe
Hi Peter,
excellent photo from this little chapel, L
All the best, Florian


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Date: 1868 ca.
Description: House of Timothy Brown II and Elizabeth Barnard Brown, 116 East Gorham Street. The house was built in 1863.
Date: 1856
Description: Painted portrait of John Arndt resting his hands on a cane.
Date: 1899-07-23
Description: Syl sleeping on a high-backed, upholstered, carved wood settee. Framed pictures are on the wall above him.
Date: 1874
Description: This map of Wisconsin shows cities, counties, railroads already completed and railroads projected, and geological makeup of the land such as lakes.
Date: 1881
Description: Cover of an advertising brochure for the Saint Paul harvester and cord binder, manufactured by the Saint Paul Harvester Works. The cover features a chromol...
Date: 1866 ca.
Description: A map of Lafayette County, Wisconsin, showing the townships and ranges, sections, towns, villages, stream and rivers, mounds, mineral deposits and the Min...


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Marci Ann Ingram, 41 of Searcy, was sentenced to 24 months in prison followed by three years of supervised release for mail fraud related to Landmark Closing Co., Inc. She used stolen escrow funds to buy Razorback tickets, gamble, shop and subscribe to Netflix.
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From Wikipedia, the free encyclopedia Polymerase chain reaction Polymerase chain reaction alternately heating and cooling the PCR sample to a defined series of temperature steps. These thermal cycling steps are necessary to physically separate the strands (at high temperatures) in a DNA double helix (DNA melting) used as the template during DNA synthesis (at lower temperatures) by the DNA polymerase to selectively amplify the target DNA. The selectivity of PCR results from the use of primers that are complementary to the DNA region targeted for amplification under specific thermal cycling conditions. Developed in 1984 by Kary Mullis,[1] PCR is now a common and often indispensable technique used in medical and biological research labs for a variety of applications.[2][3] These include DNA cloning for sequencing, DNA-based phylogeny, or functional analysis of genes; the diagnosis of hereditary diseases; the identification of genetic fingerprints (used in forensic sciences and paternity testing); and the detection and diagnosis of infectious diseases. In 1993 Mullis was awarded the Nobel Prize in Chemistry for his work on PCR.[4] A strip of eight PCR tubes, each containing a 100μl reaction. In molecular biology, the polymerase chain reaction (PCR) is a technique to amplify a single or few copies of a piece of DNA across several orders of magnitude, generating millions or more copies of a particular DNA sequence. The method relies on thermal cycling, consisting of cycles of repeated heating and cooling of the reaction for DNA melting and enzymatic replication of the DNA. Primers (short DNA fragments) containing sequences complementary to the target region along with a DNA polymerase (after which the method is named) are key components to enable selective and repeated amplification. As PCR progresses, the DNA generated is itself used as a template for replication, setting in motion a chain reaction in which the DNA template is exponentially amplified. PCR can be extensively modified to perform a wide array of genetic manipulations. Almost all PCR applications employ a heatstable DNA polymerase, such as Taq polymerase, an enzyme originally isolated from the bacterium Thermus aquaticus. This DNA polymerase enzymatically assembles a new DNA strand from DNA building blocks, the nucleotides, by using single-stranded DNA as a template and DNA oligonucleotides (also called DNA primers), which are required for initiation of DNA synthesis. The vast majority of PCR methods use thermal cycling, i.e., PCR principles and procedure Figure 1a: An old thermal cycler for PCR 1 From Wikipedia, the free encyclopedia Polymerase chain reaction but Mn2+ can be utilized for PCRmediated DNA mutagenesis, as higher Mn2+ concentration increases the error rate during DNA synthesis[7] • Monovalent cation potassium ions. The PCR is commonly carried out in a reaction volume of 10-200 μl in small reaction tubes (0.2-0.5 ml volumes) in a thermal cycler. The thermal cycler heats and cools the reaction tubes to achieve the temperatures required at each step of the reaction (see below). Many modern thermal cyclers make use of the Peltier effect which permits both heating and cooling of the block holding the PCR tubes simply by reversing the electric current. Thin-walled reaction tubes permit favorable thermal conductivity to allow for rapid thermal equilibration. Most thermal cyclers have heated lids to prevent condensation at the top of the reaction tube. Older thermocyclers lacking a heated lid require a layer of oil on top of the reaction mixture or a ball of wax inside the tube. Figure 1b: An older model three-temperature thermal cycler for PCR PCR is used to amplify specific regions of a DNA strand (the DNA target). This can be a single gene, a part of a gene, or a non-coding sequence. Most PCR methods typically amplify DNA fragments of up to 10 kilo base pairs (kb), although some techniques allow for amplification of fragments up to 40 kb in size.[5] A basic PCR set up requires several components and reagents.[6] These components include: • DNA template that contains the DNA region (target) to be amplified. • Two primers that are complementary to the 3' (three prime) ends of each of the sense and anti-sense strand of the DNA target. • Taq polymerase or another DNA polymerase with a temperature optimum at around 70°C. • Deoxynucleoside triphosphates (dNTPs; also very commonly and erroneously called deoxynucleotide triphosphates), the building blocks from which the DNA polymerases synthesizes a new DNA strand. • Buffer solution, providing a suitable chemical environment for optimum activity and stability of the DNA polymerase. • Divalent cations, magnesium or manganese ions; generally Mg2+ is used, Procedure The PCR usually consists of a series of 20 to 40 repeated temperature changes called cycles; each cycle typically consists of 2-3 discrete temperature steps. Most commonly PCR is carried out with cycles that have three temperature steps (Fig. 2). The cycling is often preceded by a single temperature step (called hold) at a high temperature (>90°C), and followed by one hold at the end for final product extension or brief storage. The temperatures used and the length of time they are applied in each cycle depend on a variety of parameters. These include the enzyme used for DNA synthesis, the concentration of divalent ions and dNTPs in the reaction, and the melting temperature (Tm) of the primers.[8] • Initialization step: This step consists of heating the reaction to a temperature of 94–96°C (or 98°C if extremely thermostable polymerases are used), which is held for 1–9 minutes. It is only required for DNA polymerases that require heat activation by hot-start PCR.[9] • Denaturation step: This step is the first regular cycling event and consists of heating the reaction to 94–98°C for 20–30 seconds. It causes melting of DNA template and primers by disrupting the hydrogen bonds between complementary 2 From Wikipedia, the free encyclopedia Polymerase chain reaction allowing annealing of the primers to the single-stranded DNA template. Typically the annealing temperature is about 3-5 degrees Celsius below the Tm of the primers used. Stable DNA-DNA hydrogen bonds are only formed when the primer sequence very closely matches the template sequence. The polymerase binds to the primer-template hybrid and begins DNA synthesis. • Extension/elongation step: The temperature at this step depends on the DNA polymerase used; Taq polymerase has its optimum activity temperature at 75–80°C,[10][11] and commonly a temperature of 72°C is used with this enzyme. At this step the DNA polymerase synthesizes a new DNA strand complementary to the DNA template strand by adding dNTPs that are complementary to the template in 5' to 3' direction, condensing the 5'-phosphate group of the dNTPs with the 3'-hydroxyl group at the end of the nascent (extending) DNA strand. The extension time depends both on the DNA polymerase used and on the length of the DNA fragment to be amplified. As a ruleof-thumb, at its optimum temperature, the DNA polymerase will polymerize a thousand bases per minute. Under optimum conditions, i.e., if there are no limitations due to limiting substrates or reagents, at each extension step, the amount of DNA target is doubled, leading to exponential (geometric) amplification of the specific DNA fragment. • Final elongation: This single step is occasionally performed at a temperature of 70–74°C for 5–15 minutes after the last PCR cycle to ensure that any remaining single-stranded DNA is fully extended. • Final hold: This step at 4–15°C for an indefinite time may be employed for shortterm storage of the reaction. To check whether the PCR generated the anticipated DNA fragment (also sometimes referred to as the amplimer or amplicon), agarose gel electrophoresis is employed for size separation of the PCR products. The size(s) of PCR products is determined by comparison with a DNA ladder (a molecular weight marker), which contains DNA fragments of known size, run on the gel alongside the PCR products (see Fig. 3). Figure 2: Schematic drawing of the PCR cycle. (1) Denaturing at 94–96°C. (2) Annealing at ~65°C (3) Elongation at 72°C. Four cycles are shown here. The blue lines represent the DNA template to which primers (red arrows) anneal that are extended by the DNA polymerase (light green circles), to give shorter DNA products (green lines), which themselves are used as templates as PCR progresses. bases of the DNA strands, yielding single strands of DNA. • Annealing step: The reaction temperature is lowered to 50–65°C for 20–40 seconds 3 From Wikipedia, the free encyclopedia Polymerase chain reaction involves spatial separation of PCR-setup areas from areas for analysis or purification of PCR products, and thoroughly cleaning the work surface between reaction setups. Primer-design techniques are important in improving PCR product yield and in avoiding the formation of spurious products, and the usage of alternate buffer components or polymerase enzymes can help with amplification of long or otherwise problematic regions of DNA. Application of PCR Isolation of genomic DNA Figure 3: Ethidium bromide-stained PCR products after gel electrophoresis. Two sets of primers were used to amplify a target sequence from three different tissue samples. No amplification is present in sample #1; DNA bands in sample #2 and #3 indicate successful amplification of the target sequence. The gel also shows a positive control, and a DNA ladder containing DNA fragments of defined length for sizing the bands in the experimental PCRs. PCR allows isolation of DNA fragments from genomic DNA by selective amplification of a specific region of DNA. This use of PCR augments many methods, such as generating hybridization probes for Southern or northern hybridization and DNA cloning, which require larger amounts of DNA, representing a specific DNA region. PCR supplies these techniques with high amounts of pure DNA, enabling analysis of DNA samples even from very small amounts of starting material. Other applications of PCR include DNA sequencing to determine unknown PCR-amplified sequences in which one of the amplification primers may be used in Sanger sequencing, isolation of a DNA sequence to expedite recombinant DNA technologies involving the insertion of a DNA sequence into a plasmid or the genetic material of another organism. Bacterial colonies (E.coli) can be rapidly screened by PCR for correct DNA vector constructs[15]. PCR may also be used for genetic fingerprinting; a forensic technique used to identify a person or organism by comparing experimental DNAs through different PCRbased methods. Some PCR 'fingerprints' methods have high discriminative power and can be used to identify genetic relationships between individuals, such as parent-child or between siblings, and are used in paternity testing (Fig. 4). This technique may also be used to determine evolutionary relationships among organisms. PCR stages The PCR process can be divided into three stages: Exponential amplification: At every cycle, the amount of product is doubled (assuming 100% reaction efficiency). The reaction is very sensitive: only minute quantities of DNA need to be present.[12] Levelling off stage: The reaction slows as the DNA polymerase loses activity and as consumption of reagents such as dNTPs and primers causes them to become limiting. Plateau: No more product accumulates due to exhaustion of reagents and enzyme. PCR optimization In practice, PCR can fail for various reasons, in part due to its sensitivity to contamination causing amplification of spurious DNA products. Because of this, a number of techniques and procedures have been developed for optimizing PCR conditions.[13][14] Contamination with extraneous DNA is addressed with lab protocols and procedures that separate pre-PCR mixtures from potential DNA contaminants.[6] This usually Amplification and quantitation of DNA Because PCR amplifies the regions of DNA that it targets, PCR can be used to analyze 4 From Wikipedia, the free encyclopedia Polymerase chain reaction PCR in diagnosis of diseases PCR allows early diagnosis of malignant diseases such as leukemia and lymphomas, which is currently the highest developed in cancer research and is already being used routinely. PCR assays can be performed directly on genomic DNA samples to detect translocation-specific malignant cells at a sensitivity which is at least 10,000 fold higher than other methods. PCR also permits identification of non-cultivatable or slow-growing microorganisms such as mycobacteria, anaerobic bacteria, or viruses from tissue culture assays and animal models. The basis for PCR diagnostic applications in microbiology is the detection of infectious agents and the discrimination of nonpathogenic from pathogenic strains by virtue of specific genes. Viral DNA can likewise be detected by PCR. The primers used need to be specific to the targeted sequences in the DNA of a virus, and the PCR can be used for diagnostic analyses or DNA sequencing of the viral genome. The high sensitivity of PCR permits virus detection soon after infection and even before the onset of disease. Such early detection may give physicians a significant lead in treatment. The amount of virus ("viral load") in a patient can also be quantified by PCRbased DNA quantitation techniques (see below). Figure 4: Electrophoresis of PCR-amplified DNA fragments. (1) Father. (2) Child. (3) Mother. The child has inherited some, but not all of the fingerprint of each of its parents, giving it a new, unique fingerprint. extremely small amounts of sample. This is often critical for forensic analysis, when only a trace amount of DNA is available as evidence. PCR may also be used in the analysis of ancient DNA that is tens of thousands of years old. These PCR-based techniques have been successfully used on animals, such as a forty-thousand-year-old mammoth, and also on human DNA, in applications ranging from the analysis of Egyptian mummies to the identification of a Russian Tsar.[16] Quantitative PCR methods allow the estimation of the amount of a given sequence present in a sample – a technique often applied to quantitatively determine levels of gene expression. Real-time PCR is an established tool for DNA quantification that measures the accumulation of DNA product after each round of PCR amplification. • See also Use of DNA in forensic entomology Variations on the basic PCR technique • : This diagnostic or cloning technique is used to identify or utilize single-nucleotide polymorphisms (SNPs) (single base differences in DNA). It requires prior knowledge of a DNA sequence, including differences between alleles, and uses primers whose 3' ends encompass the SNP. PCR amplification under stringent conditions is much less efficient in the presence of a mismatch between template and primer, so successful amplification with an SNP-specific primer signals presence of the specific SNP in a sequence.[17] See SNP genotyping for more information. • or Polymerase Cycling Assembly (PCA): Assembly PCR is the artificial synthesis of long DNA sequences by 5 From Wikipedia, the free encyclopedia performing PCR on a pool of long oligonucleotides with short overlapping segments. The oligonucleotides alternate between sense and antisense directions, and the overlapping segments determine the order of the PCR fragments thereby selectively producing the final long DNA product.[18] : Asymmetric PCR is used to preferentially amplify one strand of the original DNA more than the other. It finds use in some types of sequencing and hybridization probing where having only one of the two complementary strands is required. PCR is carried out as usual, but with a great excess of the primers for the chosen strand. Due to the slow (arithmetic) amplification later in the reaction after the limiting primer has been used up, extra cycles of PCR are required.[19] A recent modification on this process, known as Linear-After-The-Exponential-PCR (LATEPCR), uses a limiting primer with a higher melting temperature (Tm) than the excess primer to maintain reaction efficiency as the limiting primer concentration decreases mid-reaction.[20] : This technique is similar to traditional PCR, but uses a constant temperature rather than cycling through denaturation and annealing/extension cycles. DNA Helicase, an enzyme that unwinds DNA, is used in place of thermal denaturation.[21] : This is a technique that reduces nonspecific amplification during the initial set up stages of the PCR. The technique may be performed manually by heating the reaction components to the melting temperature (e.g., 95˚C) before adding the polymerase.[22] Specialized enzyme systems have been developed that inhibit the polymerase's activity at ambient temperature, either by the binding of an antibody[9] or by the presence of covalently bound inhibitors that only dissociate after a high-temperature activation step. Hot-start/cold-finish PCR is achieved with new hybrid polymerases that are inactive at ambient temperature and are instantly activated at elongation temperature. (ISSR): a PCR method for DNA fingerprinting that amplifies regions between some simple sequence repeats to produce a unique fingerprint of amplified fragment lengths.[23] Polymerase chain reaction • : a method used to allow PCR when only one internal sequence is known. This is especially useful in identifying flanking sequences to various genomic inserts. This involves a series of DNA digestions and self ligation, resulting in known sequences at either end of the unknown sequence.[24] • : This method uses small DNA linkers ligated to the DNA of interest and multiple primers annealing to the DNA linkers; it has been used for DNA sequencing, genome walking, and DNA footprinting.[25] • (MSP): The MSP method was developed by Stephen Baylin and Jim Herman at the Johns Hopkins School of Medicine,[26] and is used to detect methylation of CpG islands in genomic DNA. DNA is first treated with sodium bisulfite, which converts unmethylated cytosine bases to uracil, which is recognized by PCR primers as thymine. Two PCRs are then carried out on the modified DNA, using primer sets identical except at any CpG islands within the primer sequences. At these points, one primer set recognizes DNA with cytosines to amplify methylated DNA, and one set recognizes DNA with uracil or thymine to amplify unmethylated DNA. MSP using qPCR can also be performed to obtain quantitative rather than qualitative information about methylation. • : Miniprimer PCR uses a novel thermostable polymerase (S-Tbr) that can extend from short primers ("smalligos") as short as 9 or 10 nucleotides, instead of the approximately 20 nucleotides required by Taq. This method permits PCR targeting smaller primer binding regions, and is particularly useful to amplify unknown, but conserved, DNA sequences, such as the 16S (or eukaryotic 18S) rRNA gene. 16S rRNA miniprimer PCR was used to characterize a microbial mat community growing in an extreme environment, a hypersaline pond in Puerto Rico. In that study, deeply divergent sequences were discovered with high frequency and included representatives that defined two new division-level taxa, suggesting that miniprimer PCR may reveal new dimensions of microbial diversity.[27] By enlarging the "sequence space" that may be queried by PCR primers, this technique • • • • 6 From Wikipedia, the free encyclopedia may enable novel PCR strategies that are not possible within the limits of primer design imposed by Taq and other commonly used enzymes. (MLPA): permits multiple targets to be amplified with only a single primer pair, thus avoiding the resolution limitations of multiplex PCR (see below). : The use of multiple, unique primer sets within a single PCR mixture to produce amplicons of varying sizes specific to different DNA sequences. By targeting multiple genes at once, additional information may be gained from a single test run that otherwise would require several times the reagents and more time to perform. Annealing temperatures for each of the primer sets must be optimized to work correctly within a single reaction, and amplicon sizes, i.e., their base pair length, should be different enough to form distinct bands when visualized by gel electrophoresis. : increases the specificity of DNA amplification, by reducing background due to non-specific amplification of DNA. Two sets of primers are being used in two successive PCRs. In the first reaction, one pair of primers is used to generate DNA products, which besides the intended target, may still consist of non-specifically amplified DNA fragments. The product(s) are then used in a second PCR with a set of primers whose binding sites are completely or partially different from and located 3' of each of the primers used in the first reaction. Nested PCR is often more successful in specifically amplifying long DNA fragments than conventional PCR, but it requires more detailed knowledge of the target sequences. : is a genetic engineering technique allowing the construction of a DNA sequence with an alteration inserted beyond the limit of the longest practical primer length. : is used to measure the quantity of a PCR product (preferably real-time). It is the method of choice to quantitatively measure starting amounts of DNA, cDNA or RNA. Q-PCR is commonly used to determine whether a DNA sequence is present in a sample and the number of its copies in the sample. The method with currently the highest level of accuracy is Quantitative real-time PCR. It is often Polymerase chain reaction confusingly known as RT-PCR (Real Time PCR) or RQ-PCR. QRT-PCR or RTQ-PCR are more appropriate contractions. RTPCR commonly refers to reverse transcription PCR (see below), which is often used in conjunction with Q-PCR. QRT-PCR methods use fluorescent dyes, such as Sybr Green, or fluorophorecontaining DNA probes, such as TaqMan, to measure the amount of amplified product in real time. : (Reverse Transcription PCR) is a method used to amplify, isolate or identify a known sequence from a cellular or tissue RNA. The PCR is preceded by a reaction using reverse transcriptase to convert RNA to cDNA. RT-PCR is widely used in expression profiling, to determine the expression of a gene or to identify the sequence of an RNA transcript, including transcription start and termination sites and, if the genomic DNA sequence of a gene is known, to map the location of exons and introns in the gene. The 5' end of a gene (corresponding to the transcription start site) is typically identified by an RT-PCR method, named RACE-PCR, short for Rapid Amplification of cDNA Ends. : encompasses multiple meanings, including Polony Amplification (where PCR colonies are derived in a gel matrix, for example), 'Bridge PCR' (the only primers present are covalently linked to solid support surface), conventional Solid Phase PCR (where Asymmetric PCR is applied in the presence of solid support bearing primer with sequence matching one of the aqueous primers) and Enhanced Solid Phase PCR[28] (where conventional Solid Phase PCR can be improved by employing high Tm solid support primer with application of a thermal 'step' to favour solid support priming). : Thermal asymmetric interlaced PCR is used to isolate unknown sequence flanking a known sequence. Within the known sequence TAIL-PCR uses a nested pair of primers with differing annealing temperatures; a degenerate primer is used to amplify in the other direction from the unknown sequence.[29] : a variant of PCR that aims to reduce nonspecific background by gradually lowering the annealing temperature as • • • • • • • • • 7 From Wikipedia, the free encyclopedia PCR cycling progresses. The annealing temperature at the initial cycles is usually a few degrees (3-5˚C) above the Tm of the primers used, while at the later cycles, it is a few degrees (3-5˚C) below the primer Tm. The higher temperatures give greater specificity for primer binding, and the lower temperatures permit more efficient amplification from the specific products formed during the initial cycles.[30] • : This method uses isothermal conditions for amplification, and may be used in living cells.[31][32] • : this method allows genome walking and genetic fingerprinting using a more specific 'two-sided' PCR than conventional 'one-sided' approaches (using only one gene-specific primer and one general primer - which can lead to artefactual 'noise') [33] by virtue of a mechanism involving lariat structure formation. Streamlined derivatives of UFW are LaNe RAGE (lariat-dependent nested PCR for rapid amplification of genomic DNA ends) [34], 5'RACE LaNe [35] and 3'RACE LaNe [36]. Polymerase chain reaction dramatic improvements of the PCR method. The DNA polymerase isolated from T. aquaticus is stable at high temperatures remaining active even after DNA denaturation,[11] thus obviating the need to add new DNA polymerase after each cycle[3]. This allowed an automated thermocycler-based process for DNA amplification. When Mullis developed the PCR in 1983, he was working in Emeryville, California for Cetus Corporation, one of the first biotechnology companies. There, he was responsible for synthesizing short chains of DNA. Mullis has written that he conceived of PCR while cruising along the Pacific Coast Highway one night in his car.[39] He was playing in his mind with a new way of analyzing changes (mutations) in DNA when he realized that he had instead invented a method of amplifying any DNA region through repeated cycles of duplication driven by DNA polymerase. In Scientific American, Mullis summarized the procedure: "Beginning with a single molecule of the genetic material DNA, the PCR can generate 100 billion similar molecules in an afternoon. The reaction is easy to execute. It requires no more than a test tube, a few simple reagents, and a source of heat."[40] He was awarded the Nobel Prize in Chemistry in 1993 for his invention,[4] seven years after he and his colleagues at Cetus first put his proposal to practice. However, some controversies have remained about the intellectual and practical contributions of other scientists to Mullis' work, and whether he had been the sole inventor of the PCR principle. (see main article: Kary Mullis) History A 1971 paper in the Journal of Molecular Biology by Kleppe and co-workers first described a method using an enzymatic assay to replicate a short DNA template with primers in vitro.[37] However, this early manifestation of the basic PCR principle did not receive much attention, and the invention of the polymerase chain reaction in 1983 is generally credited to Kary Mullis.[38] At the core of the PCR method is the use of a suitable DNA polymerase able to withstand the high temperatures of >90°C (>195°F) required for separation of the two DNA strands in the DNA double helix after each replication cycle. The DNA polymerases initially employed for in vitro experiments presaging PCR were unable to withstand these high temperatures.[2] So the early procedures for DNA replication were very inefficient, time consuming, and required large amounts of DNA polymerase and continual handling throughout the process. The discovery in 1976 of Taq polymerase (a DNA polymerase purified from the thermophilic bacterium, Thermus aquaticus, which naturally occurs in hot (50 to 80 °C (120 to 175 °F)) environments[10]) paved the way for Patent wars The PCR technique was patented by Kary Mullis and assigned to Cetus Corporation, where Mullis worked when he invented the technique in 1983. The Taq polymerase enzyme was also covered by patents. There have been several high-profile lawsuits related to the technique, including an unsuccessful lawsuit brought by DuPont. The pharmaceutical company Hoffmann-La Roche purchased the rights to the patents in 1992 and currently holds those that are still protected. A related patent battle over the Taq polymerase enzyme is still ongoing in several jurisdictions around the world between Roche and Promega. The legal arguments have extended beyond the lives of the original PCR 8 From Wikipedia, the free encyclopedia and Taq polymerase patents, which expired on March 28, 2005[41] Polymerase chain reaction References [1] [2] [3] [4] [5] [6] [7] [8] [9] ^ D.J. Sharkey, E.R. Scalice, K.G. Christy Jr., S.M. Atwood, and J.L. Daiss (1994). "Antibodies as Thermolabile Switches: High Temperature Triggering for the Polymerase Chain Reaction". Bio/ Bartlett & Stirling (2003)—A Short History of the Technology 12: 506–509. doi:10.1038/ Polymerase Chain Reaction. In: Methods Mol nbt0594-506. Biol. 226:3-6 [10] ^ Chien A, Edgar DB, Trela JM (1976). ^ Saiki, RK; Scharf S, Faloona F, Mullis KB, Horn "Deoxyribonucleic acid polymerase from GT, Erlich HA, Arnheim N (December 20 1985). the extreme thermophile Thermus "Enzymatic amplification of beta-globin genomic aquaticus". J. Bacteriol 174: 1550–1557. sequences and restriction site analysis for PMID 8432. diagnosis of sickle cell anemia". Science 230 [11] ^ Lawyer FC, Stoffel S, Saiki RK, Chang (4732): 1350–4. doi:10.1126/science.2999980. SY, Landre PA, Abramson RD, Gelfand PMID 2999980. http://sunsite.berkeley.edu/cgiDH (1993). "High-level expression, bin/ebind2html/pcr/034. purification, and enzymatic ^ Saiki, RK; Gelfand DH, Stoffel S, characterization of full-length Thermus Scharf SJ, Higuchi R, Horn GT, Mullis aquaticus DNA polymerase and a KB, Erlich HA (1988). "Primer-directed truncated form deficient in 5' to 3' enzymatic amplification of DNA with a exonuclease activity". PCR Methods thermostable DNA polymerase". Science Appl. 2: 275–287. PMID 8324500. 239: 487–91. doi:10.1126/ [12] Campbell Biology,7th edition science.2448875. PMID 2448875. [13] PCR from problematic templates. Focus http://sunsite.berkeley.edu/cgi-bin/ 22:1 p.10 (2000). ebind2html/pcr/009. [14] Helpful tips for PCR. Focus 22:1 p.12 ^ Karry Mullis Nobel Lecture, December (2000). 8, 1993 [15] Pavlov AR, Pavlova NV, Kozyavkin SA, Cheng S, Fockler C, Barnes WM, Higuchi Slesarev AI (2006). "Thermostable DNA R (1994). "Effective amplification of long Polymerases for a Wide Spectrum of targets from cloned inserts and human Applications: Comparison of a Robust genomic DNA". Proc Natl Acad Sci. 91: Hybrid TopoTaq to other enzymes". in 5695–5699. doi:10.1073/ Kieleczawa J. DNA Sequencing II: pnas.91.12.5695. PMID 8202550. Optimizing Preparation and Cleanup. ^ Joseph Sambrook and David W. Russel Jones and Bartlett. pp. 241–257. ISBN (2001). Molecular Cloning: A Laboratory 0-7637-3383-0. Manual (3rd ed.). Cold Spring Harbor, [16] "Chemical Synthesis, Sequencing, and N.Y.: Cold Spring Harbor Laboratory Amplification of DNA (class notes on Press. ISBN 0-87969-576-5. Chapter 8: MBB/BIO 343)". Arizona State In vitro Amplification of DNA by the University. Polymerase Chain Reaction http://photoscience.la.asu.edu/photosyn/ Pavlov AR, Pavlova NV, Kozyavkin SA, courses/BIO_343/lecture/DNAtech.html. Slesarev AI (2004). "Recent Retrieved on 2007-10-29. developments in the optimization of [17] Newton CR, Graham A, Heptinstall LE, thermostable DNA polymerases for Powell SJ, Summers C, Kalsheker N, efficient applications". Trends Smith JC, and Markham AF (1989). Biotechnol. 22: 253–260. doi:10.1016/ "Analysis of any point mutation in DNA. j.tibtech.2004.02.011. PMID 15109812. The amplification refractory mutation Rychlik W, Spencer WJ, Rhoads RE system (ARMS)". Nucleic Acids Research (1990). "Optimization of the annealing 17 (7): 2503–2516. doi:10.1093/nar/ temperature for DNA amplification in 17.7.2503. PMID 2785681. vitro". Nucl Acids Res 18: 6409–6412. [18] Stemmer WP, Crameri A, Ha KD, doi:10.1093/nar/18.21.6409. PMID Brennan TM, Heyneker HL (1995). 2243783. "Single-step assembly of a gene and http://www.pubmedcentral.nih.gov/ entire plasmid from large numbers of articlerender.fcgi?tool=pubmed&pubmedid=2243783. oligodeoxyribonucleotides". Gene 164: 9 From Wikipedia, the free encyclopedia 49–53. doi:10.1016/ 0378-1119(95)00511-4. PMID 7590320. [19] Innis MA, Myambo KB, Gelfand DH, Brow MA. (1988). "DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA". Proc Natl Acad Sci USA 85: 9436–4940. doi:10.1073/ pnas.85.24.9436. PMID 3200828. [20] Pierce KE and Wangh LJ (2007). "Linearafter-the-exponential polymerase chain reaction and allied technologies Realtime detection strategies for rapid, reliable diagnosis from single cells". Methods Mol Med. 132: 65–85. doi:10.1007/978-1-59745-298-4_7. PMID 17876077. [21] Myriam Vincent, Yan Xu and Huimin Kong (2004). "Helicase-dependent isothermal DNA amplification". EMBO reports 5 (8): 795–800. doi:10.1038/ sj.embor.7400200. [22] Q. Chou, M. Russell, D.E. Birch, J. Raymond and W. Bloch (1992). "Prevention of pre-PCR mis-priming and primer dimerization improves low-copynumber amplifications". Nucleic Acids Research 20: 1717–1723. doi:10.1093/ nar/20.7.1717. PMID 1579465. [23] E. Zietkiewicz, A. Rafalski, and D. Labuda (1994). "Genome fingerprinting by simple sequence repeat (SSR)anchored polymerase chain reaction amplification". Genomics 20 (2): 176–83. doi:10.1006/geno.1994.1151. [24] Ochman H, Gerber AS, Hartl DL (1988). "Genetic applications of an inverse polymerase chain reaction". Genetics 120: 621–623. PMID 2852134. [25] Mueller PR, Wold B (1988). "In vivo footprinting of a muscle specific enhancer by ligation mediated PCR". Science 246: 780–786. doi:10.1126/ science.2814500. PMID 2814500. [26] Herman JG, Graff JR, Myöhänen S, Nelkin BD, Baylin SB (1996). "Methylation-specific PCR: a novel PCR assay for methylation status of CpG islands". Proc Natl Acad Sci USA 93 (13): 9821–9826. doi:10.1073/ pnas.93.18.9821. PMID 8790415. [27] Isenbarger TA, Finney M, Ríos-Velázquez C, Handelsman J, Ruvkun G (2008). "Miniprimer PCR, a new lens for viewing the microbial world". Applied and Environmental Microbiology 74: 840–9. Polymerase chain reaction doi:10.1128/AEM.01933-07. PMID 18083877. [28] Khan Z, Poetter K, Park DJ (2008). "Enhanced solid phase PCR: mechanisms to increase priming by solid support primers". Analytical Biochemistry 375: 391–393. doi:10.1016/j.ab.2008.01.021. PMID 18267099. [29] Y.G. Liu and R. F. Whittier (1995). "Thermal asymmetric interlaced PCR: automatable amplification and sequencing of insert end fragments from P1 and YAC clones for chromosome walking". Genomics 25 (3): 674–81. doi:10.1016/0888-7543(95)80010-J. [30] Don RH, Cox PT, Wainwright BJ, Baker K, Mattick JS (1991). "'Touchdown' PCR to circumvent spurious priming during gene amplification". Nucl Acids Res 19: 4008. doi:10.1093/nar/19.14.4008. PMID 1861999. http://www.pubmedcentral.nih.gov/ pagerender.fcgi?artid=328507&pageindex=1. [31] David, F.Turlotte, E., (1998). "An Isothermal Amplification Method". C.R.Acad. Sci Paris, Life Science 321 (1): 909–914. [32] Fabrice David (September-October 2002). "Utiliser les propriétés topologiques de l'ADN: une nouvelle arme contre les agents pathogènes" (PDF). Fusion. http://www.lab-rechassociatives.com/pdf/ Utiliser%20la%20Topologie%20de%20l'ADN.pdf. (in French) [33] Myrick KV, Gelbart WM (2002). "Universal Fast Walking for direct and versatile determination of flanking sequence". Gene 284: 125–131. doi:10.1016/S0378-1119(02)00384-0. PMID 11891053. [34] Park DJ Electronic Journal of Biotechnology (online). 15 August 2005, vol. 8, no. 2 [35] Park DJ (2005). "A new 5' terminal murine GAPDH exon identified using 5'RACE LaNe". Molecular Biotechnology 29: 39–46. doi:10.1385/MB:29:1:39. PMID 15668518. [36] Park DJ (2004). "3'RACE LaNe: a simple and rapid fully nested PCR method to determine 3'-terminal cDNA sequence". Biotechniques 36: 586–588,590. PMID 15088375. [37] Kleppe K, Ohtsuka E, Kleppe R, Molineux I, Khorana HG (1971). "Studies on 10 From Wikipedia, the free encyclopedia Polymerase chain reaction polynucleotides. XCVI. Repair Scientific American 262 (4): 56–61, replications of short synthetic DNA's as 64–5. catalyzed by DNA polymerases". J. Mol. [41] Advice on How to Survive the Taq Wars Biol. 56: 341–361. doi:10.1016/ ¶2: GEN Genetic Engineering News 0022-2836(71)90469-4. Biobusiness Channel: Article. May 1 http://www.sciencedirect.com/ 2006 (Vol. 26, No. 9). science?_ob=ArticleURL&_udi=B6WK7-4DKM9WR-36&_user=103681&_coverDate=03%2F14%2F19 info(%23toc%236899%231971%23999439997%23524037%23FLA%23display%23Volume)&_cdi=689 [38] Rabinow, Paul (1996). Making PCR: A • PCR Virtual Lab Story of Biotechnology. Chicago: • US Patent for PCR University of Chicago Press. ISBN • Step-through animation of PCR - Cold 0-226-70146-8. Spring Harbor Laboratory Adobe Flash [39] Mullis, Kary (1998). Dancing Naked in required the Mind Field. New York: Pantheon • PCR at Home - performing PCRs with lowBooks. ISBN 0-679-44255-3. cost household materials Scientific [40] Mullis, Kary (1990). "The unusual origin American of the polymerase chain reaction". External links Retrieved from "http://en.wikipedia.org/wiki/Polymerase_chain_reaction" Categories: Molecular biology, Polymerase chain reaction, Laboratory techniques, DNA profiling techniques, Amplifiers, Hoffmann-La Roche, Biotechnology, Molecular biology techniques, American inventions This page was last modified on 17 May 2009, at 12:17 (UTC). All text is available under the terms of the GNU Free Documentation License. (See Copyrights for details.) Wikipedia® is a registered trademark of the Wikimedia Foundation, Inc., a U.S. registered 501(c)(3) taxdeductible nonprofit charity. 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Family Finds Scissors In Birthday Cake
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Yoly Nava and her family were celebrating her mother's birthday in Arlington, Texas, when their time was ruined by a pair of scissors baked into a store-bought cake. Yoly's brother bought the $59.99 tres leches cake from the nearby El Rancho Supermarket, and nowhere on the receipt did it mention anything about a free prize hidden inside.
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"Couldn't ask for a finer salad. Elegant and delicious. I used a mix of romaine, arugula, and spinach (what I had in the fridge). Awesome good." – naple34102
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CHICAGO, July 31- CBOE Holdings Inc, operator of the largest U.S. stock options market, reported stronger-than-expected quarterly earnings and revenue on Friday due to increased transaction fees and regulatory fines imposed against customers. The average revenue per contract rose 14 percent to 36.8 cents from a year earlier as clients shifted to more...» Read More
Feb 4- CME Group Inc announced on Wednesday that it would shutter most of its open-outcry trading pits by July 2, bringing an end to the once-raucous tradition of face-to-face price discovery that has been in decline since the rise of computerized trading. Following are key dates in the 167- year history of the exchange, according to the exchange operator:.
CHICAGO, Feb 4- CME Group Inc will shutter most of its open-outcry futures pits by July 2, the world's largest futures market operator said on Wednesday, bringing down the curtain on a once-raucous tradition that has been in decline since the rise of computerized trading. The decision ousts traders of products ranging from grain and livestock to gold from...
Feb 4- Most open-outcry futures trading pits in Chicago and New York will close by July 2 due to almost all trading having moved to electronic platforms, the CME Group Inc said on Wednesday. Only about 1 percent of total futures volume takes place in the pits where traders shout and use hand signals to conduct business. The S&P 500 futures and options pits will...
Stung by the loss of the IPO of Chinese e-commerce firm Alibaba, the Hong Kong stock exchange took a step towards loosening its listing rules.
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CBOE won a long-running court battle to prevent rival International Securities Exchange from listing options on two key stock market indexes.
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The U.S. must do more than the recently passed "fiscal cliff" measures if the country is to rescue its Aaa debt rating from its current negative outlook, rating agency Moody's Investors Service said on Wednesday.


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The Rangers signed Armando Galarraga
Feb 3, 2014, 4:49 PM EDT
Do something famous — or almost famous, depending on your point of view — and you will always be remembered. Or, at the very least, you will always have your minor league signings for purposes of organizational depth noted on blogs. Such is the case for Armando Galarraga, who was just signed to a minor league deal with the Rangers.
He should find a nice comfy place to rent in Round Rock, because it's doubtful he'll see much action for Texas. Or, if he does, it will mean that the Rangers season has gone awry. He hasn't pitched in the bigs since 2012 and when he did, for a terrible Astros team, he put up a 6.75 ERA and walked more guys than he struck out in five starts.
- Joe - Feb 3, 2014 at 5:03 PM
This begs the question, What are the Rangers aiming for, exactly?
- hardjudge - Feb 3, 2014 at 7:31 PM
What are the Rangers aiming for, well, in this case, minor league pitching help.
- dylanthom2013 - Feb 3, 2014 at 7:39 PM
Like every team looks for this time of year.
- doctorofsmuganomics - Feb 3, 2014 at 10:30 PM
Damn you Jim Joyce. Damn you to hell.
…Whew, I hadn't said that in a while. Thanks for the reminder Craig.
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Ghana's mixed fortune as a dumping ground for e-waste
Return to Kuwait: The class of 1990
10 Apr 2013 20:57 GMT | Sport, Football, China, Iran, Japan
Japan's Vegalta Sendai stunned FC Seoul with their first ever AFC Champions League victory on Wednesday as they ignited their hopes of reaching the knock-out phase on debut.Former Japan international Atsushi Yanagisawa headed the game's only goal on 16 minutes as Sendai prevented Seoul's early qualification and rose to within two points of the Group E leaders with two games to go."It was a historic day as we won a game in Asia. I feel like we can move up (to the knock-out round)," said Sendai's jubilant coach Makoto Teguramori.But Sendai's heroics were matched by Thailand's Buriram United, who drew level with the Japanese team on points as they upset China's Jiangsu Sainty 2-0 at their rocking home stadium.
"It was a historic day as we won a game in Asia. I feel like we can move up (to the knock-out round)"
Sendai coach Makoto Teguramori
Elsewhere, J-League champions Sanfrecce Hiroshima earned their first point in a 1-1 draw with Pohang Steelers, saving themselves from an early exit. Also in Group G, Uzbekistan's Bunyodkor beat Beijing Guoan 1-0 to go two points clear.At Sendai Stadium, the hosts' Brazilian striker Wilson hit the bar in the eighth minute but it was Yanagisawa who sent the home fans into ecstasy when he headed in from a Ryang Yong-Gi corner.Seoul came close to the equaliser in the second half when Dejan Damjanovic struck the bar, while Mauricio Molina's shot was saved and Kim Ju-Young's header went wide.In Buriram, backed by their noisy home fans, the hosts took the lead in the first half through Suchao Nutnum, and Charyl Chappuis fired the second on the hour mark when he stretched to meet a ball behind the defence from Kai Hirano.But the Swiss-born player's joy was tempered when he injured his right knee during his celebration and had to be stretchered from the pitch.In Group G, Naoki Ishihara sparked hopes of a Sanfrecce win when he scored on 62 minutes, but the lead was quickly snuffed out by Hwang Jin-Sung's tap-in for Pohang five minutes later.In Wednesday's later matches, Saudi's Al Shabab reached the last 16 from Group A after beating Tractorsazi of Iran 1-0. Fellow Saudi side Al Ahli also made the knockout stages with a 4-1 thumping of Iranian team Sepahan in Group C to register their fourth win in four matches.
The top two from eight four-team groups will make up next month's last 16, played over two legs home and away. The competition then takes a break to avoid the harsh Middle East summer, before resuming for the quarter-finals in August.
Source: AFP
Crowds are kept apart as Jiangsu Sainty and Vegalta Sendai meet in AFC Champions League at scene of notorious massacre.
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Chihuahua/Toy Dachsund (Chiweenie) puppy
Cutest little female chiweenie EVER! So sweet and loving. 11 weeks old. Has had first set of shots/deworming. Has been started on potty training outside. Loves her little crate and goes for walks in her harness, too! Available to a good home. Asking $250.
Jul 29 -
Stunning salt and pepper miniature schnauzer puppy 9 weeks old
We have salt and pepper miniature schnauzer puppy available last one of the litter. He has a great disposition and loves playing with children.
He has a fabulous coat, will grow to be about 12 to 15 pounds we own both mom and dad and I are present in our home to look at at your convenience if interested.
Jul 13 -
Small liver colored miniature schnauzer female puppy!!
We have a beautiful small liver colored miniature schnauzer female puppy now available to a pre approved pet home. She has been raised with our two children five-year-old and seven-year-old, along with other dogs and even a cat. She has a great disposition, temperament and a coat to die for!!! She is going to be a looker, and a show stopper! So if you are looking for a great addition to your family she would be a fantastic prospect for you, you will stay small my guess is about 10 pounds but maybe even smaller. Don't hesitate to long!
We own both mom and dad, mom had a beautiful litter ...
Jul 17 -
Port Orchard
Purebred Blue Nose Pit Bull Puppies
Litter of adorable PUREBRED PURPLE RIBBON blue nosed pit bulls. Up to date shots and papered. ****We only have two males, one blue, one tan and one tan female left**** Contact for more pictures! Or contact to set up a time to come meet the puppies and see which one is a match for you! Sire and Dam are ON SITE: Both are Purple Ribbon Breeds Sire is Blue Gotti, Muggleston, and Razors Edge Dam is Blue Gotti, Butthead, and Razors Edge
******Litter and Parents are registered with the UKC.
First photo: Blue fawn male
Second photo: ...
Jul 20 -
Cattle dog
8 adorable puppies purebred! They were born on May 23 and will be ready to go to there forever home on July 18! We have both parents. We are now taking reservations. Six females two males.
This ad was posted with the eBay Classifieds mobile app.
Jun 24 -
Toy Aussie/ Australian Cattle dog mix male pup.
7 week old born june 11th.
Can be registered as parents are. He would be registered with the DBR as a texas heeler.
He is the last one, I was going to keep him but I better not just due to not enough time.
He's doing great with potty training and he's incredibly smart.
Super fun and loving!
Hes ready for his new home.
Last picture taken Aug. 2nd
Aug 2 -
Salem :
Farm dogs from great parents ready for work or play. First shot wormed well socialized. Red and blue Call email or text this # 541-860-8035 for more info
Jul 24 -
Eugene :
Myrtle Creek
Pups are 8 weeks they come with full registration papers I have 1 male and 1 female left to choose from . Come picks yours out! 503-843-2806. Or 971-237-2677 male on left female on right in photo
Jul 20 -
Salem :
Firecreek Farm is proudly offering another litter of quality Australian Cattle Dog puppies that were born Monday July 27, 2015. These puppies are blue, we do not breed reds. ***There is 1 female left and 3 males left*** 9 puppies in this litter.
Both sire and dam have been Xrayed for hip and elbow health. Both have even temperaments and great work ethic. We have successfully placed each puppy ...
Jul 11 -
Spokane :
We have 3 beautiful baby Australian cattle dogs. 1boy 2girls pick of the litter. High drive blood line. These are impressive animals. They are very command oriented. Extremely responsive. Great with people pets etc. The true blue working Australian cattle dog These are highly intelligent pups. They prefer to be doing some type of task throughout the day .parents are pure breed australian ...
Jul 8 -
Spokane :
1red male left! Ready to go july 4th 2015! Will be in seattle on 7/10 can deliver!
Beautiful purebred Australian cattle dog (heeler) pups. Litters due in Aug 2015, now taking deposits. These pups come from working ranch dog lines but also make great family pets, agility dogs, flyball dogs, and even BIRD dogs...that's right we have even had pups go on to be trained bird hunting/retrieving ...
Jun 26 -
Spokane :
Deer Park
541 280 1537 http://rightwayranch.wordpress.com/ We raise old style Queensland Heelers in standard miniature & toy sizes. we have pups available or coming up most of the time if not we have a waiting list. we live in Central Oregon we come to Sandy (Portland area) every few weeks & can bring pups. we have raised Heelers since the 1960's & have old style dogs from different imported lines. ...
Jun 23 -
Portland (OR) :
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text: The hotel is set round a fabulous swimming pool and gardens. Our room was on the 2nd floor overlooking the pool and had a large balcony, perfect for enjoying sundowners after a hot day visiting the temples. The room had everything we needed and the shower gels and shampoo provided were gorgeous. Every night when we went to bed there... More