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Lab 4, Appendix 1: The Polymerase Chain Reaction APPENDIX 1: THE POLYMERASE CHAIN REACTION The polymerase chain reaction, or PCR, is a technique that allows for the amplification of a specific target DNA sequence within a larger population of DNA (such as the human genome). Using PCR, picogram quantities of target DNA can be amplified to yield microgram quantities for subsequent biochemical analysis. In order to perform PCR, the DNA sequence flanking both sides of the target must be known. (Note that PCR cannot be used as a substitute for cloning unknown genes!) This information is used to synthesize short oligonucleotides (single-stranded DNA, ~20 nucleotides long) that are complementary to these flanking sequences. These oligonucleotides will be used as "primers" to prime replication of the target DNA by DNA polymerase. (Note in the diagrams below that these primers are complementary to opposite strands of the double helix and thus their 3' ends point towards each other when annealed to their complementary sequence.). It should be easy to follow the first cycle of the replication process. The target DNA is denatured into single strands by incubation at 94C, the primers are allowed to anneal to their complementary sequences by reducing the temperature to 60C, and finally the temperature is elevated to 72C to allow DNA polymerase to replicate the target by extending from the 3' end of the primers. (The exact temperatures for each of these steps vary between different PCR protocols and are dependent on the nucleotide composition of the primers being used. The temperatures given here are those used in our ABO gene PCR protocol.) region of target gene 5' 3' 3' 5' denature 5' 3' 3' 5' anneal primers to sequences flanking gene 5' 3' 3' 5' 5' 3' 3' 5' extend from primers with DNA polymerase 5' 3' 3' 5' 5' 3' 3' 5' What has just been described is one cycle of replication. The "chain reaction" part of PCR involves repeated cycles of replication, involving repeated denaturation, annealing, and extension steps, so that the replication products of one cycle become templates for replication in the next cycles. The result is an exponential increase in the amount of target DNA. The next cycle of PCR goes as follows: 33 Lab 4, Appendix 1: The Polymerase Chain Reaction denature 5' 3' 3' 5' 5' 3' 3' 5' anneal primers 5' 3' 5' 3' 5' 3' 3' 5' 5' 3' 3' 5' 3' 5' 3' 5' extend from primers with DNA polymerase 5' 5' 3' 5' 3' 5' 5' 3' 5' 3' 5' 5' After two cycles of PCR, we have four double-stranded DNA molecules containing the gene we are amplifying. After another round, we would double that number again to eight. On your own, diagram the steps and products of another cycle of PCR. This procedure could be performed using DNA polymerase from E. coli and by transferring the reaction tube to different water baths to achieve the various temperature shifts. However, this would be a very laborious procedure because you would have to shift tubes between water baths once every minute for 2-4 hours. It would also be very costly because every time you denatured the DNA at 94C, you would also irreversibly inactivate the polymerase, and would have to add fresh enzyme prior to the next extension step. Despite these inherent problems, PCR has gained widespread usage because of two developments that alleviate these problems. First, automated temperature cyclers (thermocyclers) alleviate the need of excessive manipulations by the investigator. Second, the use of a heat-stable DNA polymerase alleviates the need to add fresh enzyme prior to each extension step. This heat-stable polymerase was isolated from the thermophilic bacterium Thermus aquaticus and is called Taq DNA Polymerase. A single aliquot of Taq DNA Polymerase added at the beginning of the protocol will remain active through at least 30 cycles of PCR. 34
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antique wall-mount or high arch faucet for cottage kitchen, apron front sinks for cottage kitchens, cottage kitchen sinks, freestanding farmhouse drainboard sink for cottage kitchens, freestanding farmhouse sinks for cottage kitchens, how to cottage kitchen faucets, how to cottage kitchen sinks, old-fashioned faucet for cottage kitchen, trough sinks for cottage kitchens
seriously (almost) my dream kitchen down to the farm sink!! maybe a little bigger kitchen island! and diffrent color cabinets
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kitchen | modern rustic | beams | ceiling | wood. Rustic spaces could be elegant and chic as well. Get it to your living room, bedroom or even dining room. Learn how to create the best ambiences! Check out www.pinterest.com... for more amazing ideas.
[CasaGiardino] Marble slab counter top on island, marble tiles on back splash...dark wood accents...stainless steel hood, pendant lights and stove. Almond stained cabinets with French patisserie hanging racks. Love the plank panel detail on the island. Black legged bar stools pick up on the rear black counter. Perfect.
{décor inspiration | places : chandeliers & carrara in the kitchen} by {this is glamorous}, via Flickr
Check out that cute chalkboard to use for reminders or grocery needs in this immaculate white kitchen.
rethinking sparkly counters, and leaning towards white and gray marble for when we have a home again
I like this size of opening into the kitchen... where it's not totally open (I need kitchen wall space...) but the traffic flow is good. And I love the beams.
farm house. I would LOVE a sink like this in a mudroom. Perfect for so many things, and multiple messy hands from playing outside.

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Job Searches Beyond the Big Cities: Finding Employment in Medium to Small-Sized MarketsWould you like to learn the best ways to search for jobs in medium and smaller-sized cities and towns? In this book, you wil get information essential to understanding the unique nature of securing interviews and jobs in these markets. You will find job search tools that will enable you to develop a larger pool of job opportunities than you thought possible. Also, learn about online job resources.
$25.99If you're embarking upon a career in interior design, here's a highly visual overview of the profession, with in-depth material on educational requirements, design specialties, finding a job, and the many directions a career in interior design can take. Featuring informative interviews with working designers, this Second Edition includes updated educational requirements and a list of accredited
The Resume Handbook: How to Write Outstanding Resumes and Cover Letters for Every SituationOnly 1 interview is granted for every 250 resumes received-with The Resume Handbook, you can make sure yours is the one on top! Your resume has one purpose: to obtain an interview. In order to create an interview-winning resume, you need to know what to say and how to say it-and The Resume Handbook will show you how. The book focuses on three major objectives:Organization: Give your resume
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Horizon Shipbuilding, Inc., Bayou La Batre, Ala., has delivered the M/V Capt. Kirby Dupuis to Florida Marine Transporters of Mandeville, La. She joins her sister ship, the M/V Capt. W. D. Nunley in pushing cargoes throughout the inland waterways of the United States.
One of the largest Italian tug owners, Fratelli Neri S.p.A, ordered its first Damen tug, an ASD 3212, on 21 July 2015.
The vessel will be the first ASD tug
Shell Oil's icebreaker MSV Fennica weaved through nine remaining protesters hanging from the St. Johns Bridge and made its way toward the Pacific Ocean.
James Troop supplies two new Volvo Penta engines to upgrade Mersey pilot boat
Merseyside-based engine specialist James Troop & Co has secured a deal to supply


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The Ten Commandments
10 Commandments
10 Commandments
Everyone knows about the Ten Commandments. But what do they really mean? For many people they're just a list of "thou shalt nots." Old fashioned bible talk that has as much relevance to modern day life as a butter churn. In fact, less than half of Americans today couldn't even name half of the 10 commandments.
We made it simple. We've given the 10 Commandments an extreme makeover.
Here's the Ten Commandments stripped down to its true intentions. (Don't worry; we're keeping the original text too!)
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The same weekend NPR premiered the stream of Corn, a new posthumous collection from Arthur Russell, the Red Bull Music Academy assembled a live musical tribute to the "great genius" of New York's '80s music scene. Held over two nights at the Brooklyn Academy of Music, "Red Hot + Arthur Russell" featured band leader Stuart Bogie and numerous musicians, including Sam Amidon, Cults, Lonnie Holley, Devonté Hynes, Redding Hunter, Little Scream, Thao Nguyen (of Thao & The Get Down Stay Down), Richard Reed Parry (of Arcade Fire), Rubblebucket, Jake Shears (of Scissor Sisters) and Grey McMurray performing songs from the late, great Russell. Many of those tributes were released as part of the Master Mix compilation from Yep-Roc last year.
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Mavis Staples and the Flaming Lips were among the lineup for the 2015 Nelsonville Music Festival, an annual four-day event produced by Stuart's Opera House, "a historic, non-profit theater located in Nelsonville [Ohio]". Both acts returned to the festival after previous appearances in recent years. The Flaming Lips performed a memorable show in 2011, and Staples began to perform in 2013 before her set was interrupted by a thunderstorm. That both artists were interested in another visit to Nelsonville is a testament to the quality of the festival and its growing reputation as a place for music and art enthusiasts to enjoy eclectic programming.
Iceland's Ólafur Arnalds continues to gain renown with his diverse solo and collaborative projects. His For Now I am Winter was well-reviewed, he's done the soundtrack for the detective drama Broadchurch, and his neo-classical project with Alice Sara Ott, The Chopin Project is one of the most intriguing albums I've come across in 2015. Kiasmos, a collaboration with Janus Rasmussen, a member of electro-pop Bloodgroup, is a project that began in 2009. But the duo only released their first full-length album last year. Though I didn't discover it till this year, I was fortunate enough to catch Kiasmos in New York on a rare tour—one that will wrap up soon with a couple California shows before a few European dates (listed below).
Memorial Day Weekend kicked off in style at the Boston Calling Festival. The three-day event, set beneath the buildings surrounding City Hall Plaza, received strong performances from headliners Beck, My Morning Jacket, and the Pixies, with a well-rounded supporting cast that included Tame Impala, St. Vincent, and TV on the Radio.
Radio 104.5 in Philadelphia celebrated their 8th birthday with an awesome lineup of bands. Specifically, Of Monsters and Men, Death Cab For Cutie, Hozier, Passion Pit, Walk the Moon, AWOLNATION, Vance Joy, Coasts, Meg Myers, X Ambassadors, Night Riots, Holychild and Andorra. Held just outside Philly in Camden, the day-long festival was packed with people, with those furthest from the stage, on the lawn, enjoying an incredible early summer day. The event felt like the unofficial start of the summer festival season in the Northeast with a variety of different kinds of music across two stages. In the Northeast, people now have many other events to look forward to including Radio 104.5's Summer Block Party shows, Boston Calling, Governors Ball, The Roots Picnic, Newport Folk Festival, Wilco's Solid Sound Fest and many, many more.
On the second stage, we caught fast-rising rock band, X Ambassadors, and promising new singer Meg Myers. On the main stage, dance pop acts Walk the Moon and Passion Pit, supporting their new album, Kindred, led into rock music from Hozier. A lot of people left following Hozier's set but the lawn still appeared packed for Death Cab for Cutie and the final band, Of Monsters and Men, who are about to release a new album Beneath the Skin. Photos from the event are below with a larger gallery on Facebook as well as some video clips we found.
Dark Palms "Locket" (audio) (premiere) // Music
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Residence Adriana, Marseille
- Free WiFi
Main amenities
- 30 smoke-free apartments
- Near the beach
- Breakfast available
- Terrace
- Daily housekeeping
- Limited hour front desk
- Tour/ticket assistance
What's around
- Heart of Carry-le-Rouet
- Martigues Garden Beach (11.6 mi / 18.6 km)
- Parish of Martigues (12.1 mi / 19.5 km)
- Parc du Griffon (13.6 mi / 21.8 km)
Within walking distance of the harbor and all its restaurants. Very nice building and…
Carre Le Rouet is such a lovey place. This hotel is perfectly located and is a 5 minute…
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Hotel Info: 866-539-8117from $75
- Standard Studio, 1 Double Bed
- Comfort Studio, 1 Double Bed
- Comfort Studio, 1 Double Bed, Terrace
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At a glance
Key facts
Hotel size
- This hotel has 30 rooms
- This hotel is arranged over 3 floors
- Check-in time 3 PM-8 PM
- Check-out time is 11 AM
Required at check in
- Credit card or cash deposit required
- Government-issued photo ID required
- Minimum check-in age is 18
- Minimum Spring Break check-in age is 18
Travelling with others
- Pets allowed *
- Free WiFi in public areas
- Free WiFi in rooms
- No parking
- Free nearby parking
Other information
- Smoke-free property
Nearby One Star Marseille hotels
In the hotel
- Buffet breakfast daily (surcharge)
- Bicycle rentals nearby
- Golf lessons available nearby
- Golfing nearby
- Scooter/moped rentals nearby
- Scuba diving nearby
- Water skiing nearby
- Front desk (limited hours)
- Tours/ticket assistance
- Number of buildings/towers - 1
- Year Built 2011
- Elevator/lift
- Terrace
- Accessible bathroom
- In-room accessibility
- Roll-in shower
In the room
- Climate control
- Blackout drapes/curtains
- Soundproofed rooms
- Private bathroom
- Bathtub only
- 52-cm plasma TV
- Satellite TV channels
- Desk
- Free WiFi
- Refrigerator
- Microwave
- Kitchenette
- Cookware, dishware, and utensils
- Daily housekeeping
- Connecting/adjoining rooms available
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Recently reviewed hotels in Marseille
- Suite Novotel Marseille Centre EuromedFrom
"Perfect room for our needs, 1 double bed plus a single bed with a sliding room divider, for me, my husband and 19 yo son. Very clean and practical room."Jul 25, 2015
- Grand Hôtel Roi René Aix-en-Provence Centre MGalleryFrom
"One of the great places to stay if you stop in beautiful Aix-En-Provence. Impeccable service, nice pool and restaurant, very quiet and residential neighborhood, yet just a few…"Jul 24, 2015
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"Excellent location for us, between the train station and town. Great customer service and concierges. Only complaints were the bathroom didn't have much counter space and the…"Jul 23, 2015
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Residence Adriana, Marseille's small print
Also known as
- Residence Adriana
- Residence Adriana Aparthotel
- Residence Adriana Aparthotel Carry-le-Rouet
- Residence Adriana Carry-le-Rouet
The property has connecting/adjoining rooms, which are subject to availability and can be requested by contacting the property using the number on the booking confirmation.
Mandatory fees
You'll be asked to pay the following charges at check in or check out:
- Resort fee: EUR 0.80 per person, per night
Optional extras
Buffet breakfast is offered for an extra charge of EUR 10 per person (approximately)
Cribs/Infant beds are available for EUR 10 per night
Pets are allowed for an extra charge of EUR 9.00 per pet, per night
We have included all charges provided to us by this hotel. However, charges can vary, for example, based on length of stay or the room you book.


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With a voice as powerful as it is sultry and sweet, Christine Sweeney deftly handles poppy and playful without sacrificing genuine emotion. Both solo and with her band, she performs her unique brand of indie acoustic pop along with an eclectic mix of covers in the New York City area.
Christine has performed in venues such as The Bitter End, Studio at Webster Hall, the gallery at CBGB's, and Trump Towers as well as various coffeehouses. Check the events calendar for a full list of upcoming dates.
Christine's band features Mike Walsh on bass, Dave Ferro on piano, Samantha Kantor on backing vocals and Hugo Lopez on drums. Her influences include Rilo Kiley, Michelle Branch, and Grace Potter.


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University of Cambridge > Talks.cam > Computer Laboratory Security Seminar > Mobile Social Networks and Context-Awareness: Usage, Privacy and a Solution to Texting While Driving?
Mobile Social Networks and Context-Awareness: Usage, Privacy and a Solution to Texting While Driving?
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If you have a question about this talk, please contact Wei Ming Khoo.
Many location-sharing systems have been developed over the past 20 years, and only recently have these systems started to be adopted by consumers. One of the major successes so far in terms of user-adoption is Foursquare, which reports having ca. 7.5 million users as of March 2011. We studied both qualitatively and quantitatively how and why people use Foursquare, and how they manage their privacy. We will report on our user studies and surprising uses of Foursquare. Furthermore, we will discuss our ongoing work in using context-awareness and location sharing to nudge people not to use their mobile phones while driving.
Speaker's bio: Janne Lindqvist is a Postdoctoral Fellow with the Human-Computer Interaction Institute at Carnegie Mellon University. Janne works at the intersection of mobile computing, systems security and human-computer interaction. His current projects include usable privacy interfaces for mobile phones, context-aware mobile mashups, and mitigating problems with mobile phone usage while driving. Before joining the academia, Janne co-founded a wireless networks company, Radionet, which was represented in 24 countries before being sold to Florida-based Airspan Networks during 2005.
This talk is part of the Computer Laboratory Security Seminar series.
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JR and Vanessa are at St Mary's Food Bank lending a helping hand and saluting organizations that make communities stronger. With school just around the corner they begin with an organization that helps kids get ready to return to school.
Stitches of Love began in 1967 when the Salt River flooded in Phoenix. Christine Wilhelm, Stitches of Love volunteer, explains a lady and her friends began sewing clothing for children needing clothes after the flood. Today Stitches of Love has over 500 volunteers.
Every month throughout the year an open house is held for volunteers to select materials to be used for a sewing or crafting project. The next month the finished item(s) are returned and held in inventory until the Back To School Clothing Drive distribution the 2nd Saturday in July. It is exciting for the kids to get their backpacks and clothing and what they need to start school. It is important for children to feel confident about going back to school. To find out more out Stitches of Love go to backtoschoolclothingdrive.com/programs/stitches-of-love
The next organization making a difference is one fostering creativity and self-expression. Art Helping Kids can be life changing.
Free Arts AZ is changing lives. Professional teaching artist Ryan Ingram, dance instructor for Free Arts Hip Hop Camp, explains the intent of the camp is to introduce kids to the Hip Hop Culture. The camp gives kids the tools and avenues to express themselves creatively through music, art and dance.
This year the camp is held at ASU for a week in July. Campers come from all over the valley and from a variety of environments; forester homes, group homes and treatment centers. The camp gives them an opportunity to interact and hear each other's story and to know they are going through similar experiences. One student has attended the camp four times and says the camp has become the family he never had. He explains it has increased his sense of confidence. Once a kid that retreated to the corner, he is more open and reaches out to others.
Ingram explains the camp helps kids find their voice. It gives them hope and opportunities they wouldn't have – it helps them heal. Visit FreeArtsAZ.org for more information.
For more than 36 years Parenting Arizona has played a vital role in the community and throughout the state of Arizona. Erika Mendoza, Parenting AZ Director with Chicanos Por La Causa shares the mission of Parenting AZ. The mission is to prevent child abuse and maltreatment of children by empowering parents to thrive. Through the organization parents are given tools and techniques to raise healthy and successful children.
In 2014 CPLC directly served 5,000 families and provided outreach to 46,000 families throughout Arizona. Parenting AZ did a survey of parents participating in the programs and services and 98% of parents reported they have been able to better support their children. Of the parents currently being served 97% indicated knowing more about child development. The services are offered statewide and are free. To learn more and be connected to the right programs visit parentingaz.org.
After 14 years of Operation Santa Claus this August will introduce the first ever Operation Summer Claus. Summer Clause will support the four charities supported by Sanderson Ford's Operation Santa Claus – Military Assistance Mission, Southwest Autism Research and Resource Center, Ronald McDonald House Charities Phoenix and St Mary's Food Bank. Max Sirstins, of Sanderson Ford, recognizes the charities have needs year round, not just at the holiday season. He notes all of the charities are in Phoenix but they reach statewide. All the money donated stays here to help the local charities and communities.
David Kimmerle, CEO of Sanderson Ford, has dubbed Max the Father of Operation Santa Claus, an event that has grown year after. For Kimmerle giving back to the community has always been a part of Sanderson Ford's 60 years of doing business. Sanderson employees are involved and the community is involved. Community helping community is a powerful thing.
August 3-9, OperationSummerClaus.com will begin accepting donations. A $10 donation is suggested but any donation is accepted and appreciated. Going on line and registering is an opportunity to be entered in a drawing to win a holiday trip to Ney York City. After August 9th the selection of a winner will be made. The prize includes airfare, four nights of hotel accommodations, $250 spending money and tickets to a Broadway show. This first ever Operation Summer Claus is a great way to help Sanderson Ford help these charities. These charities and Sanderson Ford have helped thousands of families. Visit OperationSummerClaus.com for more information on the Claus.
JR tips his hat to firefighters by putting on their hat.
Seeing what firefighters do on a daily basis is the purpose of JR's visit to the Phoenix Fire Department Academy.
JR, along with Sonya Contreras, Phoenix Fire Department Academy Media Coordinator gives viewers a glimpse of what it takes to be a firefighter.
Shelly Jamison, Battalion Chief and 15 year Phoenix Fire Department veteran runs the Public Affairs Division. She explains that the Technical Rescue Team (TRT) handles very difficult rescues; high angle rescues, swift water rescues, tight space rescues, etc. TRT handles the events the regular trucks don't do. She explains to JR that there is more to firefighting than "turning off" fires. Chief Jamison says the majority of calls are EMS, Emergency Medical Services.
All fire fighters are trained as an EMT, and many are paramedics. She notes that the trucks carry state of the art life support equipment.
Joaquin Torres, a multidiscipline firefighter, tells JR it is important to stay motivated and committed to accomplish one's dream.
After putting on 75 pounds of gear and completing a course at the academy JR gains insight and appreciation for firefighters.
Volunteering their time at St Mary's Food Bank was a way for Vanessa and JR to tip their imaginary hats to organizations that make communities stronger. They conclude the true heroes are those who do work on behalf of others.


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"Justice without force is impotent, force without justice is tyranny. Unable to make what is just strong, we have made what is strong just." ~ Blaise Pascal
What Is To Be Done? - A Comment on Angelo Codevilla's "Ruling Class"
"In his paper "America's Ruling Class – and the Perils of Revolution"1 Professor Angelo Codevilla offers an excellent analysis of the causes and forms of government encroachment into the basic traditional liberties of Americans, and a very good sketch of the reasons why big government ideology succeeded in imposing its tenets upon the country, despite overwhelming opposition by Americans. The problem America faces, according to him, is nothing less than a complete usurpation of power by an alienated elite: the ideologues of big government and the politicians that work in concert to subvert the structure of the American constitution, and to rule over the great majority of Americans against their will. In pursuing this goal they are using the federal government, judiciary and educational system as their levers of power."
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text: Quick View save 26% The Alphabet War: A Story about Dyslexia by Diane Burton Robb January 1, 2004 | Picture Books In stock online from $17.66 When Adam started kindergarten, the teacher wanted him to learn about letters. But "p" looked like "q," and "b" looked like "d." In first grade, he had to put the letters into words so he could read. That was the beginning of the Alphabet War. See Details $18.59 / $17.66 lowest price*was $23.99


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Robert Lamm
Robert Lamm Net Worth is $5 Million.. Robert William Lamm (born October 13, 1944) is an American keyboardist, singer and songwriter who came to fame as a founding member of the pop rock band Chicago. He wrote many of the band's biggest hits, including "Does Anybody Really Know Wh...
Robert Lamm Net Worth is $5 Million.Robert Lamm Net Worth is $5 Million. Robert William Lamm is an American keyboardist, singer and songwriter who came to fame as a founding member of the pop rock band Chicago. He wrote many of the band's biggest hits, including "Does Anybody Really Know What Time It Is?," "Beginnings," "Saturday in the Park," "Dialogue," and "25 or 6 to 4."
Robert Lamm was born in Brooklyn, New York and raised in Chicago, Illinois. His parents had a collection of jazz records, which were an early influence on him. He studied art in high school, particularly drawing and painting, but changed direction in college by enrolling in the music program at Roosevelt University in Chicago. In a 2003 interview, Lamm said, "My first musical training came as a member of the choir at Grace Episcopal Church, Brooklyn Heights, New York. It was a very good choir, . It exposed me to some of the great sacred music from the Middle Ages, right up th...
Robert Lamm Latest News
Did Amy Ryan blow founding Chicago keyboardist Robert Lamm?
Chicago and Earth, Wind & Fire pull into Circuit of the Americas Thursday, July 23, during their "Heart and Soul" tour. For sheer volume of chart hits that run the gamut from precision pop and ambitious jazz-rock to gloriously arranged funk, you'd be ...
Posted: July 17, 2015, 1:17 pm
Robert Lamm on Chicago's lengthy recording drought: 'That took the wind out of the band's sails'
Even as Chicago continues work on its first new material since 2006, Robert Lamm marvels over the changes that have swept through the industry. Lamm says they weren't going to make room for new songs, no matter how good they were. Over time, the impetus ...
Posted: January 5, 2014, 12:00 am
Preview: Chicago and Earth, Wind & Fire at Gexa Energy Pavilion
Keyboardist/multi-instrumentalist Robert Lamm, one of the four founding members still in the band, says he's not interested in that — or in much of anything having to do with looking back. "I've lived the life of a rock musician," the writer of ...
Posted: July 22, 2015, 1:00 am
Chicago and Earth, Wind & Fire Are an Unlikely Heavenly Pairing
Saxophonist Walter Parazaider, guitarist Terry Kath, drummer Danny Seraphine, trombonist James Pankow, and trumpet player Loughnane were attending DePaul University while keyboardist/singer Robert Lamm was attending nearby Roosevelt University. To counter ...
Posted: July 19, 2015, 8:13 pm
Whatcom County Jail report for July 16-19
Joseph James Jurich, booked by Whatcom County Sheriff's Office for failure to appear for disorderly conduct. Michael Robert Lamm, booked by Whatcom County Sheriff's Office for possession of a controlled substance and failure to appear for fourth-degree ...
Posted: July 20, 2015, 1:46 pm
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40 Miles Of 'Sticky Goo' Damages 150 Cars In Pennsylvania
We have to pass along more about this bullet from our Thanksgiving travel roundup:
"Some motorists were delayed for hours last night and early today on the Pennsylvania Turnpike near Pittsburgh when 'a tar-like substance ... leaked from a tanker,' the Pittsburgh Post-Gazette reports."
Pittsburgh's KDKA-TV says "the tanker, carrying 4,000 to 5,000 gallons of driveway sealant, got on the turnpike at the New Castle exit" in the early evening, headed southeast and didn't stop until it got to a service plaza near Oakmont, Pa. — a distance of about 40 miles according to the turnpike's website.
Apparently, the MTS Transport driver didn't realize that his tank was leaking most of the way. Now, turnpike spokesman Carl DeFebo tells the Beaver County Times, it's possible "there could be hundreds of insurance claims" against the company from drivers whose cars were damaged. CBS News says it was told by officials that "150 or more cars were disabled when the sticky goo covered their tires and wheels."
No one was injured, thankfully, and the highway was reopened by 11 p.m. ET last night thanks to "a combination of salt, sand and cinders" that was used to break up the sticky substance and snowplows that then pushed it aside, the County Times says.


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Zionist Union MK Itzik Shmuli confirms: I'm gay
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Diplomacy & Politics
03/11/2014 22:32
Palestinians celebrate prisoner release 390.
(photo credit:REUTERS)
Israel and the Palestinians are keying up for a major battle over the coming two weeks regarding whether Israeli Arabs will be included in the next security prisoner release later this month.Palestinian officials have said in recent days that they expect Israel to release Israeli Arabs in the final batch of 26 prisoners, who are set to go free on March 28. Foreign Minister Avigdor Liberman, however, made clear on Tuesday that all five Yisrael Beytenu cabinet ministers would vote against releasing Israelis convicted of terrorism if the idea came to the cabinet for a vote.Under the framework agreement from last July that led to the current Israeli-Palestinian negotiations, Israel was to release 104 convicted terrorists in four stages. Israel has already released 78 prisoners, with one tranche left to go.When the cabinet voted 13-7 in July, with two abstentions, to release the terrorists in order to move into the negotiations, there was a stipulation that while a committee of five ministers would be empowered to come up with the names for each batch, the list would have to come back to the cabinet for full approval if it contained names of Israelis.Liberman's announcement on Tuesday will make it more difficult for the list to pass the cabinet. The full cabinet does not, however, have to vote on it again if no Israelis are among those to be freed.Israeli officials have said in recent weeks that although the Palestinians are pressing Israel to include Israeli Arabs, Prime Minister Binyamin Netanyahu did not specifically commit himself to do so back in July. This position is supported in Washington.Six Israeli Arabs went free in the Gilad Schalit prisoner release.On Monday, Israel Radio quoted Palestinian Authority President Mahmoud Abbas as saying recently in private meetings that if Israel did not go through with the final prisoner release, it would constitute an abrogation of the framework agreement.US Secretary of State John Kerry considers the final prisoner release a barometer of the negotiations' progress, a State Department official told The Jerusalem Post on Tuesday.If the cabinet fails to support the release, the fate of the peace process will likely be in jeopardy, the US official asserted. The US is preparing for that eventuality, but notes that the release is part of a deal already forged – not contingent upon a future agreement.US officials' Israeli counterparts have told them that the last release of prisoners, including some of the most heinous actors, would reap few returns given the dilapidated state of the talks.Kerry is being strict with the parties, the official added, in demanding that they reach a framework for the continuation of negotiations within the original ninemonth time frame.Voices have been raised recently – including that of Deputy Foreign Minister Ze'ev Elkin – saying that Israel should not go through with the final tranche unless the Palestinians commit themselves to continuing negotiations with Israel past the April 29 deadline for the talks.Meanwhile, Netanyahu told the Likud-Beytenu faction that after recent Palestinian statements, "a deal is getting further away because of the Palestinians. They said this week that they will never recognize a Jewish state or give up the right of return. I won't bring a deal that doesn't cancel the right of return and doesn't involve the Palestinians recognizing a Jewish state. These are just fundamental conditions. The Palestinians aren't showing any sign of willingness to reach a practical and fair deal."Netanyahu was referring to a speech Abbas gave last Thursday in Ramallah, in which he said, "We shall never agree to recognize the Jewish state," and insisted that the "five million Palestinian refugees and their offspring" will have the option of "returning" to Israel and "holding Israeli citizenship."Abbas is scheduled to meet with US President Barack Obama in the White House on Monday. One Israeli official said there was concern in Jerusalem that Abbas's hard-line position was an effort to "tie his own hands before he meets Obama, and then go in and say that his hands are tied. This has been standard Palestinian operating procedure."Netanyahu is expected to raise this issue with British Prime Minister David Cameron, who is arriving on Wednesday for a 30-hour visit, and to impress upon him the need for the Europeans to pressure the Palestinians to – as one official said – "change their current behavior.""The Europeans have a role," the official said. "If the Palestinians feel that their intransigence is costless, then what motivation do they have to change their intransigence? And here the Europeans have the ability to play a more effective role if the they desire to do so."In addition to meeting Netanyahu on Wednesday, Cameron – on his first visit to Israel since becoming prime minister in 2010 – is scheduled to meet with President Shimon Peres, address the Knesset and visit Yad Vashem. He will travel to Bethlehem and meet with Abbas on Thursday.Michael Wilner in Washington and Gil Hoffman contributed to this report.
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Evergreen Line has reached an agreement with Wan Hai Lines to exchange slots on Evergreen's NSA/NSB services, as well as Wan Hai's KSS/KVS services with effect from Feb. 22, 2009.
The agreement between the two Taiwanese ocean carriers will enhance Evergreen's service network in the intra-Asia trade.
The impacted services have the following port rotations:The NSA service rotation is as follows: Osaka - Kobe - Iwakuni - Moji - Hakata - Taipei - Taichung - Kaohsiung - Nansha - Shekou - Hong Kong.
First Sailing: Uni-Perfect 1183-084S estimated arrival in Osaka on 2/28/09
The NSB service rotation is as follows: Kaohsiung - Taichung – Taipei - Hong Kong - Pasir Gudang - Tanjung Pelepas - Penang - Port Kelang - Tanjung Pelepas - Singapore - Manila First Sailing: Ital Onore 1179-021S estimated arrival in Kaohsiung on 2/22/09
The KSS service rotation is as follows: Penang - Port Klang - Pasir Gudang - Singapore - Hong Kong - Kaohsiung - Keelung - Inchon - Kwangyang - Pusan- Keelung- Kaohsiung- Hong Kong- Singapore- Port Klang-Penang.
First Sailing: Wan Hai 313 S039 estimated arrival in Inchon on 2/27/09
The KVS service rotation is as follows: Inchon - Kwangyang - Ulsan -Pusan - Keelung -Taichung-Hong Kong-Ho Chi Minh - Kaohsiung -Taichung- Keelung- Inchon .The service sector for slot exchanges is from South Bound: Hong Kong- Ho Chi Minh & North Bound :Ho Chi Minh – Kaohsiung
First Sailing: Wan Hai 202 S267 estimated arrival in Hong Kong on 2/27/09
On July 31, Border Patrol Search Trauma and Rescue (BORSTAR) agents and CBP Office of Air and Marine rescued a man who was reported missing the previous morning.
Heidmar announced the promotion of Duncan Ross to Fleet Manager.
Duncan joined Heidmar's London office in 2012 as vessel manager. Prior to joining Heidmar,
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There were no personnel injuries in the fire,
The container ships MOL Empire and Northern Democrat collided at around 1430 LT Aug 1 in Westport harbor at Port Klang, Malaysia, reportedly while mooring.
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China Shipping Container Lines has placed orders for eight 13,500 twenty-foot-equivalent container ships at Shanghai Jiangnan Changxing Shipbuilding, continuing


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The Art of WarPaperback History & Warfare (Paperback)
List price $18.61
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- Publisher: Westview Press Inc
- Format: Paperback | 384 pages
- Dimensions: 137mm x 208mm x 36mm | 431g
- Publication date: 11 February 1994
- Publication City/Country: Boulder, CO
- Illustrations note: maps, diagrams
- Sales rank: 59,662
Product description
The Art of War is almost certainly the most famous study of strategy ever written and has had an extraordinary influence on the history of warfare. The principles Sun-tzu expounded were utilized brilliantly by such great Asian war leaders as Mao Tse-tung, Giap, and Yamamoto. First translated two hundred years ago by a French missionary, Sun-tzu's Art of War has been credited with influencing Napoleon, the German General Staff, and even the planning for Desert Storm. Many Japanese companies make this book required reading for their key executives. And increasingly, Western businesspeople and others are turning to the Art of War for inspiration and advice on how to succeed in competitive situations of all kinds. Unlike most editions of Sun-tzu currently available (many simply retreads of older, flawed translations), this superb new translation makes use of the best available classical Chinese manuscripts, including the ancient "tomb text" version discovered by archaeologists at Linyi, China. Ralph Sawyer, an outstanding Western scholar of ancient Chinese warfare and a successful businessman in his own right, places this classic work of strategy in its proper historical context. Sawyer supplies a portrait of Sun-tzu's era and outlines several battles of the period that may have either influenced Sun-tzu or been conducted by him. While appreciative of the philosophical richness of the Art of War, this edition stresses Sun-tzu's practical origins and presents a translation that is both accurate and accessible.
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Ralph D. Sawyer, one of America's leading scholars in Chinese warfare, has worked extensively with major intelligence and defense agencies. After studying at MIT and Harvard and a brief stint of university teaching, Sawyer has spent the past thirty years lecturing and doing international consulting work focused on China.
Review quote
Robert L. O'Connell, author of Arms and Men: A History of Warm Weapons, and Aggression "A tour de force. Sawyer puts this most famous of the classic Chinese military writings into context and shows that Sun-tzu was not just a solitary genius, but the product of a remarkably rich martial culture." Robin D.S. Yates, Burlington Northern Professor of Asian Studies, Dartmouth College "I am convinced that this translation...will prove to be the definitive edition for many years to come." Arther Ferrill, author of The Origins of War: From the Stone Age to Alexander the Great "Fills a serious gap for anyone interested in the history of ancient warfare...a fascinating book."


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Bulgaria's Consumer Protection Commission Bans 243 Dangerous Goods
Nearly 70% of the 17,834 complaints received in Bulgaria's Commission for Consumer Protection in 2013 have been handled in favor of citizens, the institution reports.
The most frequent complaints concern faulty communication and audio-visual equipment, home appliances and services, shoes and furniture.
A total of 243 types of dangerous goods posing health risks have been banned, mostly products intended for children.
Consumers have been complaining in regards to the provision of quality tourism products. After carrying out 8990 inspections, the Consumer Protection Commission have drawn up 1034 statements.
Some 169 tourist accommodation establishments have been temporarily closed, due to providing services without the required rating or certificate of registration.
- » Monument to Elias Canetti Unveiled in Bulgaria's Ruse
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- » Wildfire Forces Evacuation of 10,000 Campers in southern France
- » Bulgarian Archaeologists Discover 11th Century Rakia Distillation Vessel
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What a useless areticle. Complete waste of space. What consumer items were identified? What areas of commerce are involved? Why were they banned? How will people who have these items know there may be a risk? Where is the list readily available (and please do not say it is in a foreign country i.e. the Independant Republic of Sofia.


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Loss of Peraltada corner unavoidable TilkeJul.17 (GMM/Inautonews.com) Renowned F1 circuit designer Hermann Tilke says the loss of the fearsome Peraltada corner from the Mexico layout was unavoidable. The legendary, sweeping, high-speed end of the lap at the Autodromo Hermanos Rodriguez ... Inautonews, 1 week ago
Tilke rejects blame for boring F1 races - Crash.net, 2 weeks ago
Mexico F1 renovations '90% complete'
LONDON, England - Mexico is right on track for its return to Formula 1 in November 2015. The Autodromo Hermanos Rodriguez, not seen on the F1 calendar since 1992, is currently being extensively renovated for its 2015 return. The fabled and ...Wheels24, 2 weeks ago Mexico F1 renovations 90pc complete Inautonews, 2 weeks ago
Tilke to start work on Baku layout
Jun.30 (GMM/Inautonews.com) Once considered an unlikely fit for formula one, Azerbaijan is racing into its grand prix future. Bernie Ecclestone has inked a deal for a street race in Baku, the oil-rich former Soviet country's capital, and asked ...Inautonews, 4 weeks ago Baku Prepares for Formula 1 Grand Prix United States Azerbaijan Chamber of Commerce, 3 weeks ago Formula 1 Grand Prix of Europe preparation in Baku Today.Az, 4 weeks ago Formula One Continues Its Death Spiral In Austria Gas 2, 1 month ago
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BMW Motorsport ahead of visit to Austria
The FINANCIAL -- After the successful weekend in Zandvoort (NL), with a top-seven shut-out on Saturday and a top-five shut-out in the race on Sunday, BMW Motorsport is stopping off in Spielberg (AT) from 31st July to 2nd August. The Red Bull Ring ...Finchannel.com, 2 days ago DTM action in the mountains: BMW Motorsport ahead of visit to Austria European Motor News, 1 day ago
Pirelli returns to Malaysia as World Superbike event main sponsor
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Dept 56 Sv Jingle Mutts Animated Musical Dogs Bark The Jingle Bells Song For Sale
Jingle Mutts
Item #4030379
Does the barking dog version of "Jingle Bells" bring back fond memories? If so...this collectible Snow Village accessory is for you because the dogs on thispiece move whilebarkingthe Jingle Bells song!!!!!
Size: 5.12 x4.33 x 5.12"
Collector Notes:
Introduced: January, 2014
Retired: December, 2014
This item is brand new, inmint condition, comes in the original packaging, has never been displayed and wasremoved from the box for inspection purposes only andcomes from a pet free, smoke free Environment.
NJ residents add 7% sales tax.
Please email me with any questions, I'm always happy to answer!
We aim to exceed your shopping expectations and hope you remember to leavepositive response to let us know that you're delighted with your 5-STAR service!
Yes, we will gladly combine shipping. Please contact us for pricing.
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Jamie Carragher admits Liverpool's Europa League 'mountain to climb' against Zenit St Petersburg
Carragher and Steven Gerrard both hopeful of an Anfield comeback
James Legge
James Legge is an online writer for The Independent
Friday 15 February 2013
Liverpool's Jamie Carragher has admitted his side have "a mountain to climb" in the Europa League after their 2-0 defeat at Zenit St Petersburg last night, but hopes another of Anfield's famous European nights can redeem them in the return leg.
Two second half goals within in three minutes from Hulk and Sergei Semak sealed the match for the hosts at the sub-zero Petrovsky Stadium last night and Carragher said his team's lack of experience showed - despite him, Steven Gerrard, Lucas Leiva, Martin Skrtel and Jose Reina boasting 435 European appearances between them.
Carragher, who announced last week that he will retire at the end of the eason, said: "It was frustrating because up until they scored it had probably been a perfect European night in the way we dealt with the onslaught in the first half and creating a few chances."
"Second half we looked in control but that's European football, we had a few experienced lads and some young lads and you have to learn but you have to learn quickly - especially at this level.
"That is a typical European night because you think you are in control and, bang, you are 2-0 down with a mountain to climb.
"But it is a mountain the club have climbed in the past and we have to look to do again in a week's time.
"It was a disappointing night but we have a week to dust ourselves down and try to come back and if we could do it it would be a great European night."
Star man Luis Suarez squandered a number of chances, with manager Brendan Rodgers admitting the Uruguayan international could have had a hat-trick in the first half. But Carragher said Suarez- the club's top scorer with 17 goals this season- should not be held responsible for the result.
"Everyone creates chances - we never put them in - but you can't be conceding goals like that, especially at that time of the game in the 70th minute when you have everything under control," he said.
"There is no point in looking at the strikers, we have to look at ourselves as a team defensively in that situation.
"The first one is a great goal to be fair but you can always defend things better but the second one is a poor goal all round for us.
"They are big favourites now, of course they are, but it will make for a good atmosphere at Anfield next week and if we can get that first goal the place will be bouncing."
These sentiments were echoed by club captain Steven Gerrard, who told the club's official website: "If anyone can pull it back, it's us.
"We'll have an advantage with our supporters. If they can create the noise they're capable of, get behind the team and we can get an early goal, it's game on.
"But we are disappointed. It wasn't the result we wanted - we wanted an away goal and to try and sneak away with a clean sheet, but if you concede sloppy goals, you get punished and that's what happened.
"Up until the 70th minute against Zenit, the game was under control. It was a tough night in tough conditions. We're disappointed with the two sloppy goals we conceded, but the tie is not over."
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Swoon: From Haiti With Love
Brooklyn based Street Artist Swoon has been in Haiti for the last few weeks helping re-build the community and bring in alternative ways of constructing housing and shelter. The project involves the residents of the village of Barriere Jeudy in the process.
To get the work done Swoon is there with a small team of friends and like minded individuals; Tod Seelie, KT Tierny, and Ben Wolf.
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- OPEN WALLS Gallery Presents: "PAIN/TING" A Group Exhibition (Berlin, Germany)
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Heaven on earth. Crystal clear waters, a fresh water stream running into the ocean that forms a lagoon, nice restaurant/cafe for food and cocktails on site, a botanical garden leading up to the beach.
This is an intimate, but fabulous beach. The fresh river running into the cove is full of fish, so take a mask & snorkel! Food a bit pricey, but tastey. Small tour buses usually show up in the afternoon, but not too busy.
The Cove is beautiful. It's very picturesque. The sand is lovely and the food stand has very good food!
We initially visited from Hotel Mockingbird Hill for a day at the beach. This was clean, with sunbeds for hire. The sea was fun, as the clear cool water from the spring nearby mixed with the warm sea. On another visit we explored the grounds as a shower and breeze had made the sea too rough - a red flag was up. We had not realised how extensive the grounds were, or how good they were for birdwatching - we saw many endemics here.
Seriously...I don't think I've ever seen a more stunning beach in my life. Protected, freshwater stream, great sand and waves, awesome trees. Truly beautiful. I loved that due to it being a private beach, there were no hucksters. $8 well spent. I really can't say enough about the splendor and beauty of the setting.The only reason this place is not rated an excellent....Service, food and lack of credit card payment option. Service sucked and was unfriendly, food was bland one day, gross another and despite having internet, electricity and a constant stream of clientele, the restaurant only accepts cash. Bogus and unacceptable in the year 2013.Bring cash or your own beer and food! Free WIFI is a plus!
If you own or manage Frenchman's Cove, register now for free tools to enhance your listing, attract new reviews, and respond to reviewers.


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Gene-based personalized medicine has many possibilities for diagnosis and targeted therapy, but one big bottleneck: the expensive and time-consuming DNA-sequencing process.
Now, researchers at the University of Illinois at Urbana-Champaign have found that nanopores in the material molybdenum disulfide (MoS2) could sequence DNA more accurately, quickly and inexpensively than anything yet available.
"One of the big areas in science is to sequence the human genome for under $1,000, the 'genome-at-home,'" said Narayana Aluru, a professor of mechanical science and engineering at the U. of I. who led the study. "There is now a hunt to find the right material. We've used MoS2 for other problems, and we thought, why don't we try it and see how it does for DNA sequencing?"
As it turns out, MoS2 outperforms all other materials used for nanopore DNA sequencing – even graphene.
A nanopore is a very tiny hole drilled through a thin sheet of material. The pore is just big enough for a DNA molecule to thread through. An electric current drives the DNA through the nanopore, and the fluctuations in the current as the DNA passes through the pore tell the sequence of the DNA, since each of the four letters of the DNA alphabet – A, C, G and T – are slightly different in shape and size.
Most materials used for nanopore DNA sequencing have a sizable flaw: They are too thick. Even a thin sheet of most materials spans multiple links of the DNA chain, making it impossible to accurately determine the exact DNA sequence.
Graphene has become a popular alternative, since it is a sheet made of a single layer of carbon atoms – meaning only one base at a time goes through the nanopore. Unfortunately, graphene has its own set of problems, the biggest being that the DNA sticks to it. The DNA interacting with the graphene introduces a lot of noise that makes it hard to read the current, like a radio station marred by loud static.
MoS2 is also a single-layer sheet, thin enough that only one DNA letter at a time goes through the nanopore. In the study, the Illinois researchers found that DNA does not stick to MoS2, but threads through the pore cleanly and quickly. See an animation online.
"MoS2 is a competitor of graphene in terms of transistors, but we showed here a new functionality of this material by showing that it is capable of biosensing," said graduate student Amir Barati Farimani, the first author of the paper.
Most exciting for the researchers, the simulations yielded four distinct signals corresponding to the bases in a double-stranded DNA molecule. Other systems have yielded two at best – A/T and C/G – which then require extensive computational analysis to attempt to distinguish A from T and C from G.
The key to the success of the complex MoS2 simulation and analysis was the Blue Waters supercomputer, located at the National Center for Supercomputing Applications at the U. of I.
"These are very detailed calculations," said Aluru, who is also a part of the Beckman Institute for Advanced Science and Technology at the U. of I. "They really tell us the physics of the actual mechanisms, and why MoS2 is performing better than other materials. We have those insights now because of this work, which used Blue Waters extensively."
Now, the researchers are exploring whether they can achieve even greater performance by coupling MoS2 with another material to form a low-cost, fast and accurate DNA sequencing device.
"The ultimate goal of this research is to make some kind of home-based or personal DNA sequencing device," Barati Farimani said. "We are on the path to get there, by finding the technologies that can quickly, cheaply and accurately identify the human genome. Having a map of your DNA can help to prevent or detect diseases in the earliest stages of development. If everybody can cheaply sequence so they can know the map of their genetics, they can be much more alert to what goes on in their bodies."
The Air Force Office of Scientific Research and the National Science Foundation supported this work, published in the journal ACS Nano.
Liz Ahlberg | University of Illinois at Urbana-Champaign
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Directed by Peter Jackson
I really didn't like The Hobbit: An Unexpected Journey when I first saw it.
It was in 3D, and the much vaunted 48fps ''High Frame Rate'', on the best screen in Wellington, sitting with people who had all worked on the film, and who clapped and cheered whenever one of ''their'' bits came on screen.
And so, in a spasm of professionalism, I went to see the film again. The second time around I chose a smaller cinema, showing a standard print.
And, freed from the distraction of the 3D, and the unfamiliar brightness and sharpness of the HFR, I thought the story-telling and the characters had far more resonance and power than they did with all the technological bells and whistles getting in the way.
I've asked around in the year since, and I know many people feel the same way; the HFR, combined with 3D, was a distraction for some, and the story suffered for it.
Then again, I can't look at a fluorescent tube without wanting to commit an act of violence, and yet I know people who work under them all day. So possibly I've just got a cockamamie visual cortex.
So I wasn't really looking forward to seeing The Hobbit: The Desolation of Smaug this week. Lovely though it is to be invited to a red carpet premiere, I really would rather have slipped into some provincial flea-pit to watch the film.
But the deadline wouldn't allow that, so there I was on Monday night, not holding my breath that I was going to particularly enjoy the 160 minutes to come.
But you know what? This film is better than Unexpected Journey.
In fact, The Desolation of Smaug is so good it actually gave me a fresh appreciation of what that first part of the trilogy had achieved.
If Unexpected Journey seemed to take an awfully long time to cover very little story (which is surely a peril, when making a film about short people who walk everywhere) it did at least introduce us very thoroughly to the troupe.
So that when The Desolation of Smaug kicks off with a hiss and a roar, we aren't wondering who these hairy little oddities are.
The emotional investment was made last year, and now we can sit back and thrill to the spectacle of them banging around some of the more perilous and pointy bits of Middle-Earth, knowing that most of the exposition has been dealt with, and this film can get on with the serious business of being your ticket's worth of bloody good fun.
Whereas Journey never got near repeating the tension of Bilbo meeting Gollum, Smaug has four or five scenes from which to choose your favourite.
Peter Jackson, and his co-directors Andy Serkis and Christian Rivers, have assembled a feast of battles and escapades. The story never feels perfunctory, and yet every situation leads to another scrap, another hair-raising escape.
There's magic in this film, and an economy and a leanness that the editors - I'm guessing - didn't have the time to cut into Journey.
Whereas Journey showed us every tea cup being put back on Bilbo's shelves, Smaug tells us as much as we need to know, and then tears into the next set-piece with absolute gusto.
The effect is exhilarating. At two hours and forty minutes, no one's claiming that Jackson hasn't again heroically cast off the shackles of brevity, but this time around, everything that happens on screen drives the story forward, and what isn't needed has been jettisoned.
Happily, one early victim of this new economy is the character of Radagast, who threatened to be the Jar Jar Binks of the franchise in Journey. In Smaug he gets a couple of admirably terse and necessary scenes, and then he's gone.
And the High Frame Rate and 3D that so distanced the film from me a year ago?
It's different this time around.
The brightness has been dialled back, and the beauty of the sets, the costumes, the backgrounds, and all of Weta's myriad creations is all the more spectacular for it. The effect doesn't assault us this time, it just invites us to look more closely.
I liked The Desolation of Smaug very much. Sometime next week I'm going to see it somewhere a bit smaller, in a deserted mid-morning session, in 2D.
And then I predict I will absolutely love it.
- Stuff
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'This is just what her friend needs': Gwyneth Paltrow helps Beyonce 'get her energy back as they plan four-day visit to exclusive retreat together'
They are both seemingly in need of some R&R after their recent personal dramas, so it's no surprise that Gwyneth Paltrow and Beyonce are reportedly going on a four-day retreat together.
The actress will help her A-list pal, 32, unwind with yoga classes and hikes before the Drunk in Love hitmaker begins her gruelling On the Run tour with husband Jay Z later this month.
The 41-year-old is believed to have checked the pair into an exclusive complex in California's central coast, where they are expected to meditate, go on long walks and sample local wine.
And... relax! Gwyneth Paltrow and Beyonce, pictured in 2011, are reportedly going on a four-day retreat together on California's central coast
A source told The Sun newspaper: 'Gwyneth reckons this is just what her friend needs to get her energy back.'
The relaxing getaway could be exactly what the in-demand stars need following their respective personal crises this year.
Gwyneth announced her split from husband Chris Martin in March, while Beyonce found herself at the centre of a scandal when footage emerged of her sister Solange physically attacking her husband Jay in a hotel elevator.
Calm before the storm: The actress will help her A-list pal, 32, unwind with yoga classes and hikes before the hitmaker begins her gruelling On the Run tour with husband Jay Z
Pals: The 41-year-old is believed to have checked the pair into an exclusive complex, where they are expected to meditate, go on long walks and sample local wine
The XO hitmaker has previously spoken of her affinity with the Iron Man star, revealing that they bonded over their daughters.
Beyonce has a two-year-old tot, Blue Ivy, while Gwyneth's girl, Apple, is aged 10.
She said: 'She is incredible, she's a great friend on every level.
'I think for us, protecting our daughters is so important and keeping things on our own terms, so I will still be protective and make sure [Blue Ivy] has a childhood and a great time.'
MailOnline has contacted a spokesman for the stars.
Drama: A source told The Sun: 'Gwyneth reckons this is just what her friend needs to get her energy back', after the recent spat between Jay Z and her sister Solange
Beyonce and Jay Z have already sold out gigs across the country, which could be piling on the pressure ahead of June 25.
The concert preparations are apparently so intense, it caused them to skip the wedding of Kanye West and Kim Kardashian last month.
But the no-show was interpreted as a snub by many as Jay Z was in line to be Kanye's best man at one time.
Moving on: While Gwyneth has also had a difficult time of late after announcing her split from husband Chris Martin in March
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