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A novel TMPRSS3 missense mutation in a DFNB8/10 family prevents proteolytic activation of the protein
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Pathogenic mutations in TMPRSS3, which encodes a transmembrane serine protease, cause non-syndromic deafness DFNB8/10. Missense mutations map in the low density-lipoprotein receptor A (LDLRA), scavenger-receptor cysteine-rich (SRCR), and protease domains of the protein, indicating that all domains are important for its function. TMPRSS3 undergoes proteolytic cleavage and activates the ENaC sodium channel in a Xenopus oocyte model system. To assess the importance of this gene in non-syndromic childhood or congenital deafness in Turkey, we screened for mutations affected members of 25 unrelated Turkish families. The three families with the highest LOD score for linkage to chromosome 21q22.3 were shown to harbor P404L, R216L, or Q398X mutations, suggesting that mutations in TMPRSS3 are a considerable contributor to non-syndromic deafness in the Turkish population. The mutant TMPRSS3 harboring the novel R216L missense mutation within the predicted cleavage site of the protein fails to undergo proteolytic cleavage and is unable to activate ENaC, thus providing evidence that pre-cleavage of TMPRSS3 is mandatory for normal function.
- Title
- A novel TMPRSS3 missense mutation in a DFNB8/10 family prevents proteolytic activation of the protein
- Journal
Human Genetics
Volume 117, Issue 6 , pp 528-535
- Cover Date
- 2005-10
- 10.1007/s00439-005-1332-x
- Print ISSN
- 0340-6717
- Online ISSN
- 1432-1203
- Publisher
- Springer-Verlag
- Additional Links
- Industry Sectors
- Authors
- Marie Wattenhofer (1) (7)
- Nilüfer Sahin-Calapoglu (2)
- Ditte Andreasen (3)
- Ersan Kalay (4) (8) (9)
- Refik Caylan (10)
- Bastien Braillard (1)
- Nicole Fowler-Jaeger (3)
- Alexandre Reymond (1) (5)
- Bernard C. Rossier (3)
- Ahmet Karaguzel (6)
- Stylianos E. Antonarakis (1)
- Author Affiliations
- 1. Department of Genetic Medicine and Development, University of Geneva Medical School, Geneva, Switzerland
- 7. Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), CNRS/INSERM/Université Louis Pasteur, 67404 Illkirch cedex, CU de Strasbourg, France
- 2. Department of Medical Biology, Medical School of Süleyman Demirel University, Isparta, Turkey
- 3. Pharmacology and Toxicology Institute, University of Lausanne, Switzerland
- 4. Department of Human Genetics, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
- 8. Department of Otorhinolaryngology, Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands
- 9. Department of Medical Biology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey
- 10. Department of Otorhinolaryngology, Faculty of Medicine, Karadeniz Technical University, Trabzon, Turkey
- 5. Center for Integrative Genomics, University of Lausanne, Switzerland
- 6. Department of Medical Biology, Medical School of Karadeniz Technical University, Trabzon, Turkey


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1994 Toyota Tercel in Washington, DC
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Inarticulate Southern Rockers from Oregon Curse Corrupt Church
Mike Damron isn't lying when he says, "I've never been too articulate," but that's his charm, along with his studied rasp and drawl and his Portland band's Southern grit. He isn't selling a complex worldview or even sincerity, although the song about his grandmother's death seems earnest. There aren't many shades of gray on Menace, and the people populating, say, his anti-war "Dust and Sun" (where the American soldier gets a last name but the Iraqi doesn't) are symbols, not fleshed-out individuals. I Can Like Any Sonofabitch in the House just want to deliver a fierce blast of misery. After starting out celebrating the end of a relationship, they proceed through loneliness, desperation, and death. By album's end, it feels like you've been reliving election night with a bottle of Jimmy Dean and a friend yelling "fuck you" at the TV. That phrase features prominently in "Westboro Baptist Church," the best track because it sounds like the band is having fun venting its anger at Fred Phelps and his ilk.
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DNR targets groundwater threats
Dan Gunderson
MPR News 91,3 FM
Park Rapids —A growing awareness about Minnesota's groundwater challenges is about to hit the residents of Park Rapids in the wallet.
Fees for city water are going up roughly 25 percent to help pay for a $2.5 million treatment plant. The new plant is needed because the city's old water supply has become too contaminated with nitrates from fertilizer applied by farmers in the area.
Dick Rutherford, who owns a business and two homes in town, is angry that city residents are paying for pollution caused by agriculture.
"We know where it's coming from," he said. "It's the farmers that are putting this stuff in the ground. I don't feel we should be paying for the whole thing, I don't think we should be paying for any of it. We should be billing these people that are putting the irrigators in."Irrigation is essential for growing crops in parts of Minnesota. But in the north central part of the state near this city of 3,700 residents, irrigated farm fields contribute to groundwater pollution and threaten a unique fishery.That's why what is known as the Straight River watershed, which includes Park Rapids, is one of three groundwater management areas the state Department of Natural Resources will focus on over the next year. As pressure builds on the groundwater available, the state's goal is to bring farmers, businesses, residents and officials together to figure out how to deal with it, possibly even to restrict use in some cases.Although the effects of irrigation were first talked about years ago because the Straight River, a valued trout stream, was getting damaged, the biggest single impact recently has been the need for the new water treatment plant Park Rapids will bring on line next month.The city never needed to treat its water before, said public works superintendent Scott Burlingame. It simply pumped clean plentiful water from a shallow aquifer."Water rates were cheap, everybody was happy with that," Burlingame said. "So things have changed."What changed is the level of nitrates in the water. Nitrates come mostly from the nitrogen fertilizer farmers use on irrigated potato and corn crops grown on top of the shallow aquifer.Nitrate levels in existing city wells, which tap the aquifer about 70 feet below the surface, approach or exceed the safe drinking water standard. Click to view full aquifer mapThat forced the city to drill a well into a deeper aquifer, about 120 feet down. That aquifer is at least somewhat protected from nitrate contamination by a layer of clay above it. Hydrologists hope to map the aquifer in the next few years to better understand how water moves and interacts with the shallow, polluted aquifer."There's a lot of water in that well and good quality water," Burlingame said.The new treatment plant will remove iron and manganese from the water. Those naturally occurring metals are not a health risk but can stain sinks, showers or clothing.Based on national water use averages, a typical family of four would see its annual water bill rise about $130 as a result.IRRIGATION BOOMOver the past 25 years, farmers in the Park Rapids area sank dozens of irrigation wells because water and lots of fertilizer turned poor, sandy land into highly productive farmland for potatoes and corn.DNR groundwater specialist Michele Walker said abundant groundwater only 70 feet below the surface made it easy for farmers to expand."Back in 1988 we had about 100 permits," Walker said. "Now we have about 275." The state requires a permit from the DNR for any well pumping more than 10,000 gallons a day or a million gallons per year.Nearly eight billion gallons of water are pumped from wells in the Straight River watershed each year, 90 percent of it for crop irrigation.The sandy soils drain quickly. That's good for potatoes because wet soil can cause disease."The problem is that these soils are very sandy, and if you happen to put your nitrogen out and we get a gully washer, it's going to go somewhere," Walker said.Walker said in the Straight River watershed, it's likely the nitrogen fertilizer moves down through the soil to the aquifer that in some places is only 40 feet down.Minnesota Department of Agriculture test wells show nitrate levels two to three times the safe drinking water level. Those wells are tested at the top of the aquifer, closest to the surface where nitrates are most concentrated. But in 2012, the department tested 72 private wells in Hubbard County, wells that typically tap the aquifer farther down, and still found 10 above the safe level.Elevated nitrates in drinking water are a problem because they can cause blue baby syndrome in infants under 6 months who are bottle fed.Long-term health effects for older children and healthy adults exposed to elevated levels of nitrate in their drinking water are not yet known or agreed upon in the scientific community. But the National Cancer Institute suggests a link between elevated levels of nitrate in drinking water and an increased risk of non-Hodgkin's lymphoma.Even before the creation of the DNR's groundwater management area, the state Department of Agriculture has been working with farmers in the Park Rapids area to reduce water and fertilizer use.Fifty farmers in the area took part in a monitoring and education program last year, said Luke Stuewe, who coordinates the program for the department."Sixty-five percent of the growers involved in the program found they could make adjustments to their rate and the majority of those were reductions in rate," Stuewe said. "So they found they could do something differently and ultimately have a lower amount of nitrogen going down on the field."But nitrates are a leaky nutrient, Stuewe said. That means it moves easily through the soil with water."I think even under the absolute best conditions some loss will occur," he said. "It's a necessary nutrient that we need. It's a challenging one though."One of the biggest players facing that challenge is the R.D. Offutt Company, which grows up to 9,000 acres of potatoes a year in the Park Rapids area.Those potatoes are turned into french fries at a plant just outside Park Rapids.The company has made a number of changes in potato production in the past couple of years to reduce its impact on groundwater, said Nick David, lead agronomist for Offutt."We have no benefit to putting on more water than the crop needs," he said. "There's no economic benefit to putting on more nitrogen or fungicides or herbicides on a potato crop than it needs."David says the company uses a slow release fertilizer that reduces leakage to the groundwater. It stopped heavy fertilizer applications at planting time and instead makes several small applications throughout the growing season, sometimes putting nitrogen in the irrigation water.In some fields, it plants potato varieties that need less nitrogen."We like to utilize those in areas that are sensitive, so a good example would be areas around communities, where we have wellheads. So we've been able to cut our nitrogen use by almost half on some of those fields."Despite those changes, nitrate levels in wells continue to rise. It could take years for changes on the land to improve water quality 70 feet below.TROUT STREAM AFFECTEDGroundwater quality isn't the only concern in the Straight River Watershed.Covered with a thick layer of ice and snow now, the Straight River is a favorite trout stream when the weather warms. Trout depend on cold water, and the cold water comes from underground aquifers in this area."It's an excellent fishery," said DNR area fisheries supervisor Doug Kingsley. "The quality and size structure of the fish is remarkably unique."Kingsley said the brown trout that thrive in the Straight River are threatened by rising water temperatures, something that has been happening slowly but steadily for the past 10 years.Warmer water can't hold as much oxygen so the Straight River is on the Minnesota Pollution Control Agency's new list of impaired waters for low dissolved oxygen.It's not clear why the stream is warming, but Kingsley said pumping from irrigation wells can affect water levels.Over the years the DNR has required careful monitoring of some wells near the river. But the agency has never rejected a well permit application in the watershed.Wisconsin provides ample examples of what can happen to trout streams in sandy irrigated farmland, Kingsley said."They've had instances where one of the popular trout streams in the Stevens Point, Wis., area actually stopped flowing because of a lack of groundwater. Hopefully we learn from those and take a close look at what's going on here."If groundwater pumping is causing the stream to warm, the DNR might limit how much some farmers can pump near the river. That hasn't happened yet.But its one of issues to be studied as part of the groundwater management process.Another issue likely to be raised is the cost of water used for irrigation.Most Minnesota farmers pay $140 a year for each irrigation well they have, said DNR hydrologist Darrin Hoverson."It costs many times more to turn the pivot on for one rotation than it's going to cost for water for the whole season," he said. "There will be discussion about rates. Understanding how we can best determine allowable water uses that are sustainable (is our goal), and cost may be a component of those discussions."


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+Jerry Hildenbrand This game is just RIDICULOUSLY fun, and I simply cannnot stop grinning like a complete idiot every time I play it. So, basically, it's your fault I look like an idiot. ;-p
Jerry HildenbrandShared publicly -
The good news is that Super Monsters Ate My Condo works perfectly with 4.2.2.
Adult Swim's giant, hungry monsters are back for a second helping in Super ...
5 comments
Love it. Also, +Jerry Hildenbrand , you need to grab Fruit Pop if you haven't already, along with Carnivores HD
love this game as well but does anyone else's N4 get extremely hot while playing it as well as draining the battery? i don't get the same problems on my A200 tablet.
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Find Blue River industrial buildings for lease, retail rentals, & Blue River shops. Locate a Blue River warehouse lease and any real estate for lease / investment, including industrial properties to rent and sale.
Many things should be considered when contemplating a new Blue River commercial lease and relocating to a new location. In addition to the actual dollar amount of the lease particular consideration should be made concerning the area for recruiting purposes and retaining existing employees. Study the areas available and the possibility of expansion should the business grow. If you are managing a start-up, your undertaking can be even more daunting as you may have no indication on how your business will evolve and the area you will need.
Leasing commercial real estate gives business owners, new and established, a unique opportunity to obtain the real estate necessary to conduct business whether its a retail space, land, warehouse, flex space, medical facilities, restaurants, or will build to suit real estate. Terms and conditions should be negotiable and the landowner flexible, in some cases a professional broker may be worth the cost in order to get you a better lease contract and monthly rent. While leasing is often cost-effective, it may require a large security deposit or several months rent prepaid.
Your long term business plan and Blue River real estate commitment should be aligned as most real estate leases and investments are long in duration. Business leaders need to anticipate future growth and requirements of the business to be positioned for the best and worst possible scenarios. Define the space you need and make sure the area can be designed to incorporate all your requirements. Will the layout satisfy employee expectations and will any possible employee relocation, or commute, to a new Blue River venue be achieved to everyones satisfaction. Consider proximity to facilities and amenities such as Blue River shopping, health care, childcare, etc.
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The characteristics of leukemia
● a sudden onset. Leukemia, multiple rapid-onset, short duration, particularly for multi-children and young people.
● the first symptom of fever in acute leukemia, mostly fever, can be expressed as remittent fever, missed fever, intermittent fever, or irregular fever, body temperature at 37.5 ~ 40 ℃ or higher. A sense of when cold, but not chills.
● bleeding. Is a common symptom of leukemia. Bleeding sites over the entire body, especially in the nasal cavity, mouth, gums, skin, fundus common and may also have brain, inner ear and internal organs bleeding.
● anemia. Early stage of anemia, manifested as looking white, dizziness, palpitations and so on.
● hepatosplenomegaly. 50% of leukemia patients appear hepatosplenomegaly with acute lymphoblastic leukemia Hepatosplenomegaly the most significant.
● lymph nodes. Body a wide range of lymph nodes, with acute lymphoblastic leukemia is more common, but not as acute lymphocytic CLL significant. Superficial lymph nodes in the neck, lower jaw, armpit, groin, etc., deep in the mediastinal lymph nodes and viscera in the vicinity. Swollen lymph nodes in general texture of the soft or medium hardness, the surface smooth and no tenderness, no adhesion.
● skin and mucous membrane lesions. Associated with leukemia, skin damage manifested as nodules, lumps, maculopapular rash and so on. Manifested as nasal mucosal damage, respiratory mucosa and oral mucosa and other places, such as swelling and ulcers.
● nervous system inflammation. Subarachnoid space, meninges, etc. leukocyte infiltration can occur, performance resembling brain tumors, meningitis, etc. will appear in patients with increased intracranial pressure, meningeal irritation, limb paralysis and other symptoms.
● bone and joint disease. Infiltration of bone and joint disease after bone and joint pain often occurs, performance of the sternum, humerus and the shoulder, elbow, hip, knee pain, etc. occurs, pain, occasional pain. Children with acute lymphoblastic leukemia occur more than bone and joint tenderness.
● Other. Infiltration of leukemic cells can breath, digestive and urinary and reproductive systems, as well as the eyes, the lacrimal gland and fundus, etc. can occur in patients with diffuse or nodular changes in the lungs, accompanied by pleural effusion, digestive disorders, proteinuria, hematuria, amenorrhea or excessive menstrual flow, proptosis, vision loss and other symptoms.
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What is the deal?
Cameron's veto of the European initiative was not really a veto. As critics have pointed out, vetoes stop the process in question. In Cameron's case, the process simply took a new form, shrugged its shoulders and carried on anyway. The form is that of an intergovernmental treaty. While there is still a lot of uncertainty around it, it is likely to impose rules on all 26 EU members — not just the 17 countries which use the euro.
The treaty will hold members to strict new budgetary rules, including a cap of 0.5% of GDP on their annual structural deficits, a requirement for them to keep public deficits under three per cent of GDP and a rule that insists they must submit their budgets for European approval.
While it originally appeared that just the eurozone countries would be affected by the rules, it is now likely they will be imposed on all the EU countries. Most of the non-eurozone members have plans to join the euro eventually and they want to maintain theirRead More »from Everything you need to know about the eurozone deal in five minutes


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A "league table" for prisons has been made public by the Government.
Rankings for last year have been published for the first time and show Whanganui Prison was the only jail classed as exceptional in September. By December it had lost the top spot and is listed as "exceeding".
At the bottom of the table are Hawke's Bay, Northland and Otago prisons, which "need improvement".
Corrections Minister Anne Tolley released the rankings for the year to September 2012 and the year to last December.
All 17 prisons are measured on their performance in security, assaults, drug tests and rehabilitation programmes.
There are four grades - exceptional, exceeding, effective and needs improvement. More than half of the prisons fall into the effective category.
"These first-ever performance tables are a valuable tool for Corrections and also allow communities to measure how well our prisons are operating," Tolley said.
She said the privately run Mt Eden prison was in the exceeding category, which "shows how much progress has been made by Serco".
Serco was fined $150,000 after an inmate escaped in February, a further $25,000 for releasing an inmate early and $50,000 for failing to file progress reports.
The reports will be released quarterly.
Corrections national commissioner, Corrections Services, Brendan Anstiss said most prisons were operating "effectively or better".
"These results are very good," he said.
"We haven't had a single escape, walkaway or unnatural death in all the periods reviewed."
Otago, Hawke's Bay and Northland staff were working on reducing the number of assaults on staff and prisoners, he said.
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Fund financial associate jobs1,772 Jobs
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- Financial Associate- Fund Management This position reports directly to the CFO and Controller, giving you significant interaction with the Asset and Property Management team & IRLeading Technology CompanyLeading Technology CompanyNew York, NY30+ daysSave
- Fund Administrator, Financial Reporting, Associate 2 With more than 30,495 employees across 29 countries, at State Street, our people are our greatest asset. We recognize that highly skilled, engagedState Street2.8 1,736 Reviews State StreetBoston, MA30+ daysSave
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- Hedge Fund Solutions- Financial Associate • Developing and delivering forecasting, budgeting, and financial analysis reports to drive optimization of business decision-making and planning • Analyze and prBlackstone3.9 66 Reviews BlackstoneNew York, NY30+ daysSave
- Alternatives (Hedge/Real Estate/ PE) - Financial Accounting, Control & Reporting Sr. Associate The Alternative Funds at PIMCO total over $29B in assets under management spread amongst multiple strategies (credit, volatility, opportunisticPIMCO2.6 121 Reviews PIMCONewport Beach, CA25 daysSave
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The popsicle sticks in the bucket are the chores that need to be done and then marked off on the above chart. Then, the paycheck sheet to the left gets marked with the allowance made each day. At the end of the week, it's added up and paid, and the child separates the money into the three pouches, "Spend it", "Save it", and "Give it". Such a great way to teach so many wonderful virtues. Kids Allowance Charts, Kids Chore Charts, For Kids, Gift Ideas, Chore Ideas, Favorite Pins, Kid Chores, Charts Ideas, Popsicle Sticks
Related Pins
Chore Chart made with wood, metal, and magnets. The pictures are perfect for little ones who don't read.
Toy Ransom Box - such a cute idea & will be necessary if my son is anything like i was about cleaning up!
Good cute idea to make with your kids and easy fun chore chart!! Maybe this cute idea with simple chores will help them on a daily basis to learn their own routines easier! :) love
great idea for a chore chart- lets them be hands on (amazing what kids will do when they get to add a sticker, move a magnet, etc)
Chore Pins/Magnets- Kids choose the job and how much it's worth. At the end of the week, total amount is how much allowance they receive.
DIY Chore Chart! Brilliant!!! Finally one that makes sense. I just made 1 but its on a piece of card board and i will have to make a new 1 each week!! I love that this is so much easier and reusable :)
After their "regular" chores, give kids the option to do extra chores, which will earn them a punch in their chore card. After a card is filled, they get a small reward.
Great idea to add magnets to the back and make a magnetic chore board! Let the kids help paint and decide what kind of chores should go on the pieces! Chore Ring 105
Chore list printable, going to try this to be more organized about what the kids are helping with.


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Phil Pressey: A Steal for the Boston Celtics?
Contrary to popular belief, there is not a "hidden gem" on every NBA team. The phrase is thrown around so often we, as fans, tend to slap it on any given rookie who can muster four points per game and maybe hit a big shot or two. Every team likes to think they landed the "steal of the draft" or have some future star in the making. While all players we now see as stars had to start somewhere, so did every bench warming scrub.
All I'm suggesting is that it is ridiculous when fans rave when rookies play decently. This shouldn't come as a shock to anyone, seeing as they are playing in the NBA to begin with.
So imagine my surprise when I see a player who really could be a diamond in the rough for the Boston Celtics, but no one seems to notice.
Like any other athlete, the college career of Phil Pressey stands as the best indicator of his potential and abilities. Unfortunately, Pressey's collegiate career shows a player with major potential but with major pitfalls, too. Yet despite his battles with turnovers and late-game decision making, Pressey has a rare skill that leaves me wondering how he went undrafted. Ball-handling ability is one of the most basic and crucial skills in the game. Allen Iverson made his career and compensated for his size with excellent ball-handling skills. Ask Tim Duncan how mastering the basic fundamentals of basketball can lead to success in the NBA.
In his last season as a member of the Missouri Tigers, Pressey averaged 11 points and nine assists per game and in the process, set the school record for steals. He has also gained the accolades of making the 2012-13 SEC first team, and in the 2011-12 season, he made the All Big 12 first team. The icing on the cake lies in the fact Pressey is YouTube famous, known for dunking on unsuspecting victims who are as much as a foot taller than him. His previously-mentioned crazy handles have made a bit of a splash, too.
While nasty crossovers and thunderous dunks are fun, don't expect them every game from Pressey. The NBA is obviously a much bigger place, and Pressey in no longer a big fish in a small pond. However, his upside and potential excellence can be brought out the best through the Celtics.
First of all, Pressey gets to play under the guide and influence of Rajon Rondo, one of the best point guards in the game. Rondo, a master of ball handling, seems to have seriously curbed his turnover problem from the past and could prove to be a fine teacher to Pressey. Seeing how both Pressey and Rondo have both received awards for leading their respective competition in assists, the potential for major as well as speedy improvement is certainly there. It is the similarities between the two that could make for such an excellent back court pairing someday.
Secondly, while the odds of Pressey getting a lot of minutes are slim to none, he is a member of a somewhat depleted team, especially at the point. So, for an undrafted player added based on summer league performance, he will get decent playing time from new head coach Brad Stevens. All the better if he is sent to the Celtics' NBA D-League affiliate Maine Red Claws — a team on which he could develop as well as shine
While Pressey's game is reminiscent of Nate Robinson's in many ways, his ceiling as a player is entirely up to him. That said, his "floor" is dependent on his own ability as well.
Boston hasn't unearthed the biggest diamond in the rough or discovered the next big thing. However, watch for Pressey in the coming season. He is a player with a major upside who, with the right guidance, could have a highly positive impact on the Celtics.
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Small Business
2012 has been a tough year for some SMEs, while others seem to go from strength to strength. Four Kiwi business gurus suggest some New Year's resolutions to spark growth in 2013.
Phil O'Reilly, ceo, Business NZ
- Get paid. Most SMEs are critically dependent on good cashflow, so get control of your debtors, particularly you 90-days-plus debts.
- Get a strategy. Many SMEs don't have one; they just exist, but the difference between those that survive and those that don't is often having a plan.
- Get advice. Traditional advice for small businesses comes from your mate and your accountant. but consider an informal board of advisers. Implementing a more disciplined approach to taking advice is a good idea.
- Innovate. Broadly speaking we are, as a country, not doing enough research and development. Traditionally SMEs haven't been leaders in innovation but that is changing. And it doesn't mean science. It means looking at new ways of reaching markets that can give you the competitive advantage.
- Address the internet. If you don't have an online strategy you need one. Very little happens around making any purchase decision that doesn't involve the internet these days. Not just a website but a plan for using Twitter and Facebook as well.
Nick Churchouse, Venture manager, Creative HQ, Wellington-based business incubator
- Focus on value. The past year has seen a number of awesome start-up fairy tales emerge out of start-up hubs worldwide. But the legendary tales of success tend to focus on the zeros not the metrics. Every start-up success comes from a simple value proposition, sold well to the right people. It doesn't matter what your business, you must build the value, communicate the value and deliver the value.
- Make the money come to you. 2012 saw a deepening dearth of traditional start-up funding. Phrases such as "traction is the new IP" represent a shift from early start-up seed funding to not-so-early growth funding. Turn market research into customer feedback, turn clever tech into a business model with history. Cashflow forecasts should be based on something that actually resembles cashflow. Like grumpy rhinos, investors are attracted to movement.
- Commit yourself. Part-time lovers get sidelined. Look at what you want this year and be straight up about how you can make it happen and what you have to give to it. If 2012 was a tough year, 2013 will be harder. Achieve, build and inspire.
Andrew Hawke, Business advisory partner, KPMG Christchurch
- Manage cashflow. As demand builds stock and supplies will be needed to fill orders, placing demands on cashflow. Working capital management will become critical. A rolling cashflow forecast is useful to ensure you see warning signs early.
- Focus on the bottom line. And maintain margins to ensure top line sales growth is profitable.
- Recruit well. The war on talent will become more intense. Make sure the right frameworks are in place to recruit and retain key staff.
- Keep planning. Whilst profitability levels will increase, continue to plan strategically in terms of succession plans and exit.
Kevin Andreassend, President of Auckland ICT and managing director Ice AV
- Think global. B2B and B2C around the globe are now comfortable purchasing from businesses and sellers whom they will never meet. Make them comfortable dealing with you.
- Be a specialist. Whether it's a service, product, or technology. If you are credible and easy to find on the internet, clients will contact you.
- Be an authentic presence online. What happens online dictates what happens in the real world. It used to be vice versa. Make yourself known for your insight, knowledge and authority.
- Be on film. Online videos are now a must for every company.
- Be savvy. There are rogues, fake profiles and companies. Learn how to research them and make absolutely sure you cannot be mistaken as a scammer, spammer or rogue.
- BusinessDay.co.nz
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Marton man's cloth nappy business cleans up
More interest rates cuts to come after "relentless" dairy price drops
China's stock slump hits world markets
Households save hundreds by switching power companies
Tourism industry calls for border tax to be scrapped or delayed
John Key says NZ's drug bill set to rise under TPP, but patients protected
Departing Banking Ombudsman's challenges included suicidal customers
Widow blames forestry industry for husband's death
Anne Tolley still happy for Serco to run social services for children
Blessie Gotingco's family call for public inquiry into her killer's release
American writer takes aim at Timaru cops
Former All Black Jerry Collins' orphaned baby daughter is thriving
Gender and race discrimination hurting New Zealand financially: Lawyer Mai Chen
Faulty phone charger may have sparked Wellington house fire
Wellington couple from lost memory card found in 20 minutes
AFL racism: West Coast Eagles fan says it's just banter, world is too PC
Taylor Swift is terribly calculated and mean, says Grizzly Bear's Edward Drost
American writer takes aim at Timaru cops
Green light for second season of Westside
Cardiac arrest during cosmetic surgery: overdose of local anaesthetic likely
MPs share the correct pronunciation of some common Māori place names
Shia LaBeouf says he would have killed girlfriend Mia Goth if onlookers hadn't intervened
Winston Peters kicked out of debating chamber
Hackers can use a text to infiltrate your Android smartphone
Mum saves child before being dragged to death in escalator
Three inmates among 10 arrested in Head Hunters gang raids
Jail for woman who had baby with 12-year-old boy
Meeting planned after Ihaka's aunt cautioned by employer over comments
'I'm disgusted': Ruby Rose in trouble over Floyd Mayweather photo
South Africans question legality of Richie McCaw lineout try against Springboks
Student cowers in cab as 'road rage' driver pursues pedestrian
Blessie Gotingco's family call for public inquiry into her killer's release
Search turns to NZ in international photos lost-and-found mystery
Tornado victim Benedict Dacayan lifted from ground inside two tonne portacabin
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Walk In The Same Manner As He Walked
in Religion
Please join me Tuesday March 17th at 11 am CST as I discuss how The Scriptures say we who say we abide - Ought to walk as Jesus walked. Do you keep His commandments? Do you keep His Word? Is the love of God perfected in you?
I can safely say - no- you are not there yet!!!! How did Jesus walk? How are we supposed to walk? Thats what I'm going to discuss today. Hope you can join. ~ Char
By this we know that we have come to know Him, if we keep His commandments. (1 John 2:3)
The one who says, "I have come to know Him," and does not keep His commandments, is a liar, and the truth is not in him; (1 John 2:4)
but whoever keeps His word, in him the love of God has truly been perfected. By this we know that we are in Him: (1 John 2:5)
the one who says he abides in Him ought himself to walk in the same manner as He walked. (1 John 2:6)
Dr. Price's Response to Supreme Court's Same Sex Marriage Ruling
in Christianity
Dr. Paula Price and Ap. Dwann Holmes Rollinson address the Supreme Court's ruling on same-sex marriage. Discover the true B.E.D. of Sex, how we got here as a nation and what our next steps should be to recover all.
Dr. Price's Website: http://www.drpaulaaprice.com
Ap. Dwann's Website: http://www.prophetdwann.com
Same Sex Marriage Celebrating LGBT Marriage for All with KM Davis
Join Cultural Conversations with an intimate interview with KM Davis, Founder of Davis Law & Associates. Davis is a returned special guest to Cultural Conversations and will enlighten and engage on the subject of the Supereme Court ruling making same sex marriage legal in 50 state, the path to marriage equality and her personal journey in the Lesbian Gay Bisexual Transgender (LGBT) community.
KM Davis is founder of Davis Law & Associates. She discovered her passion for helping business owners far before she realized her own entrepreneurial leanings. However, upon realizing her dual interest (and the need to now "practice what she preached") Davis's interest in business law matters has only deepened. The ability to understand the needs of business owners and their families allows Davis to consider aspects of client issues that sometimes even they haven't realized, and creates a relationship beyond attorney/client…for most clients Davis serves a more comprehensive business advisor role.
Verona Mitchell, Ph.D.
Cultural Conversations
Same Sex Marriage Agenda
in Spirituality
We are now in the official beginning of the end! With the demonic Supreme Court decision to violate our religious freedoms and stand in the face of God with disobedience now give the unclean spirits legal right to this nation. Tonight we discuss out future and what we as Christians should do about this violation of the temple of the Holy Spirit. Letters, guest, Julie and Scott are read, interviewed and opinions are given tonight.
LGBT Wins Same-Sex Marriage Supreme Court Ruling TheWWShoW
LGBT Wins Same-Sex Marriage Supreme Court Ruling TheWWShoW
I mite say something you mite not like.
The Willie Williams Radio Show
free call (347) 677-1859 to listen/talk
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in Radio
Good morning and welcome to the Seriouside of the jrilshow. We will be discussing the following topics:
SAME SEX MARRIAGE: The Supreme Court of the United States ruled that the banning of Same Sex Marriage is unconstituational.
BLACK CHURCHES BURNED: In wake of the shootings in Charleston, South Carolina, Black Churches are being burned in the south.
MALCOLM X: With the shooting of unarmed black men, the killing of the Charleston Nine,many African Americans are tired of the saying "turn the other cheek". Some are yearning for the days of Malcolm X"By Any Means Necessary".
Supreme Court Rules on Same Sex Marriage, Bobbi Kristina's Condition, + MORE
in Pop Culture
The show where the listeners have a voice is back at a NEW TIME Sunday's at **12 Noon**
Join #HolliewoodAndFriends (Hollie Wood, Venor and Miss Juddy) Live Sundays at 12 Noon ET. as they give you the latest #HotInTheBlogs topics as only they can!
Supreme Court Same-Sex Marriage Ruling.
Bobbi Kristina moved to hospice care.
Beyoncé surprises us again!
Donald Trump's ruffling feathers again.
Mimi from Love And Hip-Hop ATL confesses.
and a whole lot MORE!
Also #WhatBlowsMine (where you, the listener can call in and tell the world what's that one thing that just gets under your skin)
Tune in live Sunday afternoon at 12pm ET!
You can call 646-716-8544 just to listen or press 1 when you hear a topic you want to chime in about, we'll bring you on live.
Are you an artist? Do you know an artist? Submit music to the be played on the show email@example.com
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Same sex marriage, with Attorney, Ross B. Russell, of Hampton & Associates, P.C.
in Legal
Constitutional background and arguments over same sex marriage, with Attorney, Ross B. Russell, of Hampton & Associates, P.C. in Fort Worth, Texas.
Topics covered on this show:
Introducing Ross Russell and brief background;
Same sex marriage (SSM), decisions in Texas, 9th Circuit and the U.S. Supreme Court;
Identify and explain levels of scrutiny and process in deciding constitutional issues;
Current case pending before the U.S. Supreme Court, potential outcomes;
Alternative theory of gender discrimination, Justice Roberts stated thoughts;
Possible challenges and reactions to the U.S. Supreme Court, if in favor of SSM.
Ross Russell is a native Texan from Central Texas and his passion for Texas law and constitution drives his focus in litigation. After graduating number five in his class from Texas A&M University School of Law, he achieved the highest score on the bar exam among A&M law graduates. A long-time entrepreneur, Ross launched his own firm, Russell, PLLC, a full service general practice firm. He also works as an attorney at the Fort Worth law firm of Hampton & Associates, P.C. and is active with a variety of complex legal matters. Ross is active in the Tarrant County Bar Association and attends frequent programs and continuing legal education seminars. Ross will be a frequent guest on Texas Law Talk Radio, sharing insight in Texas law and developments with our attorney and business professional audience. For more information visit www.TheRussellLawFirm.com and www.HamptonLawOnline.com. You may also call Ross at Hampton & Associates, P.C. by dialing (817) 877-4202.
the legalization of same-sex marriages
in Spirituality
My show has been changed today to dress to topic of the legalization of same-sex marriage.. My talkshow will air today at 12:30 p.m. central time
Same-Sex Marriage in Florida
in Politics
From Alabama, Louisiana, Georgia and Mississippi, gay couples traveled to this quaint Panhandle city on Tuesday, eager to take part in a historic moment: the arrival of same-sex marriage in the Deep South."We came because it felt like home," said Virginia Jeffries, 30, of Foley, Ala. "And because if we waited for Alabama, we'd be waiting forever."
"I have to confess, when I was first approached about coming here, I thought no way," said the Rev. Dr. Jim Merritt, who moved to Pensacola several months ago to become the senior pastor of Holy Cross Metropolitan Community Church. He married his partner of 20 years on Tuesday in one of many same-sex weddings performed at the church.
A Tuesday regular at the diner, Earl Barrett, 72, and his two breakfast companions voted in favor of Florida's gay marriage ban in 2008. Look up the definition of marriage in Webster's Dictionary, they instructed, citing a core text for gay marriage opponents. (The dictionary amended the definition several years ago to include same-sex couples.)
"I'm a Roman Catholic," Barrett said. "For me, I can see how, civilly, they have the right to the benefits of marriage. But religiously, I can't support it."Tampabay.com
Join Patrick Weaver & Lynn Wheeler as we discuss the ruling of same sex marriage
in News
In a landmark opinion, a divided Supreme Court on Friday ruled that same-sex couples can marry nationwide, establishing a new civil right and handing gay rights advocates a historic victory.In the 5-4 ruling, Justice Anthony Kennedy wrote for the majority with the four liberal justices. Each of the four conservative justices wrote their own dissent.Nearly 46 years to the day after a riot at New York's Stonewall Inn ushered in the modern gay rights movement, the decision could settle one of the major civil rights fights of this era. The language of Kennedy's opinion spoke eloquently of the most fundamental values of family, love and liberty.
New Year, Same You!
in Motivation
If we are being realistic, a new year doesn't make you new. It's a new year, but you are very much the same! Tune in to find out why!
Tuesday nights @ 11 pm: NICK speaks, listens, and instructs you how to fly! Rise above your gravity!
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About the Author
During her tenure as an emergency room nurse, Mercado developed an interest in the psychological aspects of patient care and thus, began studying the mind, body, and spirit connection. At the same time, Mercado began to focus on paranormal phenomena. For over fifteen years, she has studied and attended numerous lectures and workshops on types of hauntings and the current theories behind them. Mercado, a locally published writer, has also written for The Brooklyn Baron and The Nursing Spectrum.
She is an American Heart Association Certified CPR Instructor, and operates a business with her second husband called Learning For Life, offering CPR training to hospital and hotel personnel. Mercado still lives in Gravesend with her family.
Excerpt. © Reprinted by permission. All rights reserved.
At this point in time we were still living in a three-room apartment with our two daughters. Our landlord was extremely frugal with the heat, and we were growing tired of huddling in our living room near a portable heater. We were willing to look at virtually any house within our price range, but we had only a small sum of money available for a down payment. Real-estate agents took us from house to house, showing us lovely homes that, unfortunately, required substantial deposits.
We thought we were permanently stymied until we noticed this one house on their listing. It had been there for over two years, but no one had attempted to show it to us.
"It's too old and too much trouble," one of the brokers said, barely looking up at us, "although you might actually be able to afford this one." The sarcasm in her voice was duly noted. However, we were undaunted by her negative description of the house. We wanted to see it.
So, there we were, full of anticipation, parking our car on this very ordinary block. I was in a very "up" mood that day, partly because I had hopes this would be "the" house, partly because it was fall. September and October had always been my favorite months. The smell of cool air and the vision of trees painted in glowing hues of orange and red and yellow signaled the upcoming holiday season. This particular block had many trees, all colored so vibrantly that the whole area had a beautiful orange cast to it. Newly fallen leaves were gathered in bunches near most of the houses and they crunched beneath our feet as we walked to our latest prospect. I thought of pumpkin pie and chestnuts, and my heart leapt at the thought that perhaps, at last, we had found a house we could actually purchase. Maybe we could even spend Christmas there. I had to stop myself from skipping toward our destination.
As we were walking down the street, I noticed an unusual tree. Not only were its leaves a strikingly colored mixture of orange and red, but it appeared to be almost caressing the house in front of which it was planted. Its trunk was tilted toward the house, with almost no branches extending toward the street. Its branches and foliage sprung out like long arms, almost protectively. When we realized this was the tree in front of the house we were to look at, I felt an unusual sense of peace. I was, without obvious reason, very glad this wonderful, strong tree would be part of the package. I didn't know then that this house, indeed, needed whatever protection it could get.
The house itself was one of those big, old, fat houses; the kind they simply don't build anymore. It was whitish-gray with dark green trim, and rather dingy looking.
In retrospect I could imagine someone describing it as "spooky" looking, but "spooky" was the last thing on our minds. The front yard, not very large, was unkempt. Weeds were growing along the chainlink fence that surrounded it. The four entrance steps were made out of weather-beaten, cracked cement. The wrought-iron railing attached to these steps was rusted and peeling. The front windows, many small ones separated by strips of rotting wood, were nearly opaque with dirt.
For a brief moment, my husband and I looked at each other, wondering if this was such a good idea after all, but we knew our financial situation, and neither one of us wanted to spend another winter with our not-so-friendly landlord. So we took a deep breath and rang the doorbell, half expecting it not to work. To our surprise, we were greeted by a rather pleasant, if somewhat cool, middle-aged couple. They smiled as they brought us up to the second floor of this grand old house.
As we walked up the stairs we were wondering what would be the problem this time. We'd seen so many houses that we developed a knee-jerk reaction of not getting our hopes up. It was hard not to think that, even if the price was right, there would be something terribly wrong. Perhaps there were combative neighbors, or mud in the cellar, or exposed sewer pipes in the kitchen. We'd seen it all by this time. To our surprise, this couple mentioned only one dominant problem¿the elderly couple living downstairs. They refused to leave.
"My uncle is one tough cookie," remarked the middle-aged man, his nephew, about the old gentleman inhabiting the first floor apartment. As he reached the top of the stairs, he continued, "My uncle and aunt have been living in this house for over forty years. They sold it to us a few years back and have been paying a nominal rent ever since. I guess he thought this arrangement would go on forever, but our children are grown now and my wife and I bought a house in Florida. We are anxious to leave but my uncle keeps scaring prospective buyers off by declining to move."
My husband and I certainly didn't like the sound of that.
In a few short moments, we found ourselves on the second floor, in the front living room, with windows overlooking the aforementioned beautiful tree. The decor was very bland, no pictures on the walls, no outstanding furniture. It looked like a 1950s motel room. Working our way toward the back of the house, we were introduced to the bathroom, which appeared relatively new. Green and white tiles gleamed at us, reflecting the light on the ceiling. Towels hung on the towel racks, but no pictures or designs of any sort adorned the walls. The shower curtains were, again, green and white, but with no distinguishing pattern. The bathroom was very clean, almost sanitary, but decorated with no imagination, no feeling.
Next to the bathroom was a dining area, connected to a kitchen. Dark wood cabinets dominated this room, and pink, painted-over paneling covered the walls. It wasn't a salmon pink or a "hot" pink, just a dull, almost beige pink, going from ceiling to floor. I could see the texture of the paneling showing through the paint and I wondered why anyone would impose such a nauseating color over wood. I figured I would never find curtains to match this unfortunate hue, but before I could get too upset about the dining room and kitchen, our hosts ushered us into what they termed "the playroom." There, in the back of the house, was a beautiful, spacious area, lined with double windows. It was huge.
"This was built only twenty years ago," the woman said, "and our children spent many hours happily at play here."
I must admit, it was bright and airy and pleasant. A sloped ceiling, punctuated with halogen lights, captured our interest. We imagined our own children playing under this wondrous roof.
Our excitement was palpable. That back room really piqued our interest. Our hosts then brought us up to the third floor and showed us their bedroom, located in the front of the house. It was the largest of the three rooms on that floor, but still it had a sort of restrictive quality to it. Thick, upholstery-like curtains hung on the front windows, almost completely obliterating the radiant sunshine coming through. The spread covering the queen-size bed was heavily quilted and shiny, and reminded me of unwanted afternoons spent at my grandmother's house. I could almost smell the mothballs.
The other two rooms were nearly claustrophobic in nature¿bedrooms housing the remnant memories of their now-moved-away children. The walls were devoid of decoration, except for a Charles Atlas photo cellophane-taped to one wall. Although there were still beds in each room, no pictures or shelves or toy chests were visible. No posters or telephones or any specific reminders were present. It was impossible to tell if a male or female child had occupied either of these rooms. And we didn't ask.
The rooms felt cold and dank. We kept focusing on that airy playroom on the second floor and assumed we could turn even this stark landscape into a warm habitat.
Although the atmosphere upstairs was less than desirable, it was certainly not enough to discourage us. We were finished with our tour when the wife said, "I'm sorry about my husband's aunt and uncle . . . you'd think they'd know better at their age."
"How bad can they be?" I remarked, not quite ready for her response.
"How bad? I'll tell you how bad. They intimidate everyone who comes to see this house. Especially his uncle. He's more than a 'tough cookie'¿he's nasty and impossible, and I'm getting sick and tired of it. I don't want to live in New York anymore." I could see her husband flinch at her candidness. She continued, "We've got this beautiful house in Florida that we can't move into, and he doesn't give a shit about it. If you can persuade him to move, more power to you. Even the real- estate broker has stopped sending people to see this house. I don't care if this makes us vulnerable to a lower sale price, I want out."
Her husband was clearly not happy with her outburst, but he remained silent, seemingly trying to be tolerant of her obvious frustration.
After a brief, but very pregnant, silence, I exclaimed, "Well, can we meet the ogres downstairs?"
I thought I was being cute, but it wasn't received very well, and I couldn't help but notice my husband's disapproving look. After a moment or two, our hosts took us downstairs, said their goodbyes, and left us alone at the first floor entrance. I suddenly felt frightened. I was anxious about meeting this man and felt my heart palpitate as my husband knocked on the door. I nearly expected Lurch to answer.
My husband looked at me a little strangely and asked if there was anything wrong.
"I'm scared," I said. "Aren't you?
"Hell, no," he replied. "We can probably get this house for less than we thought."
If it weren't for his attitude, I would have gone home. ...


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Ottawa Ontario, CA - Players
Welcome to the Ottawa Squash Orbit community.
Feel free to connect and play with people.
Looking to play tonight, at Carleton, anytime after 5pm. Text me, 519-540-8043. Cheers!
Planning on booking time during this week (July 13-17) for practice. If anyone is interested in joining, send me a message. Preferred time afternoons/evenings. Txs!
Just registered and this is my first post!
After a long hiatus on my squash experiences, I am resuming gaming.
My first raquet was a wooden one! That's how I first learned the game several years ago.
Looking for players for practice within the Orleans region; will drive downtown too!
hi i'm new in Ottawa and i'm looking for some international size court i was already on a ottawaU court but its dirty and in bad condition. Is there anybody who can recommand me any good squash court in Ottawa?
Hi there have not played in years so probably call me a beginner.Once my hockey season is over in April will be looking to play some squash .So hope to hear from anyone wants to play.Prefer venues near downtown.BTW im 53 years young {)
PLease contact me 613.878.1930 email@example.com
hello everyone
I am available to play squash every day from 18 to 19.30
Hello! I am a first year bio student, and am looking for someone to play with. Haven't played in a year and I miss it. I am really not looking for anything too competitive, just want to hit the ball around a bit and have some fun.
Looking for new challenges...
Just getting back into squash (haven't played for a couple of years). Looking for lunchtime (11:30 or 12:10) or after work (15:30 or 16:10) matches. I'll give you a good run but I'm no longer a highly competitive player (I am 59 after all)!
I'm around U ottawa campus all the time. for November; evenings and weekends. haven't played in a year or 2, looking to get back into it.
I'm pretty much a beginner, maybe intermediate. I'd have to play again to find out.
If you want to play thi saturday or sunday (Nov9 or 10th), msg me please


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I really hope you update soon! Please don't leave us hanging, I need to know what happens with Rosy and Micah!!
I am currently working on posting my stories on Wattpad
Location:Northern Rivers NSW Australia
Joined:2 years ago
Reading Lists
Mygypsy's Reading List42 stories
New Reading List7 stories
5 Published Works
Featured work.Mature
Stories of Us.
Social data: 9.1K reads. 430 votes. 125 comments.
Description: This story bridges the gap between To Love a Stray and Rainbow Reflection.
Other Works by Mygypsy.
Rainbow ReflectionMature
Rainbow Reflection
To Love a StrayMature Completed
To Love a Stray
Tawny Caruthers has been hounded and hunted by Stray and Clan Werecats alike since she first became a We...
Enter the Cat Chapter 1Mature Completed
Enter the Cat Chapter 1
Micah Williamson is Chief Warrior of the Williamson Clan. Sworn to protect his family clan and control t...
Just re-read RR......That Drew. What a major douche.
GregoryOdom posted a message to Mygypsy
are you going do a two of to love a Stray
Katatude4life posted a message to Mygypsy
I've come from Literotica to find "To Love A Stray" and finish reading about Rosey. Hope I find it on your site.
Mygypsy posted Rainbow Connection. to Stories of Us.
Stories of Us.
I made a cover for To Love A Stray ❤ If you want to see it, let ne know
lola_cheesecake posted a message to Mygypsy
so I was getting kind of lost as to who was who and i created a table to keep track as i read your great story. Is this what i have got so far. anyone have a more complete/correct list? All the characters are great and i don't think there are too many but some list would help to organize it in my mind. Williamson: Wade Marissa jazzy Edwin Dwayne Micah Alex Justin Donny Wilabugh: Stephen Celeste Steven Pete Malakki Andy Stephan: Burt Bella Conner Albie Callum timny Macintosh: ray rosy sam mitchel Inness: mal carlotta Robby Malcolm Chis Leon Perkins: Jake Black: kert Dave Baxter: Stan Patrick Neil lance Dorrington: drew jakob Francis: martian adriana Danny mick loci Lancaster: bud larni larna kell kerby Commings: Allen
Mygypsy added First Flight (Generation Icarus #1) to New Reading List
First Flight (Generation Icarus #1)
Mygypsy commented on The Lost Stories - The Sword Breaker Bow
Mygypsy commented on The Lost Stories - Luca's Tale