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Seattle's Big Blog
Covering Seattle news, weather, arts and conversation.
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Long-time Phinney Ridge restaurant Santa Fe Cafe is closing
The Santa Fe Cafe is closing after 30 years in business. Owner Drew Bloom announced on Facebook earlier this month that the restaurant, at 5910 Phinney Ave. N., that the restaurant would close Jan. 1 "due to mitigating circumstances." "This was obviously a tough decision and one that was not taken lightly," he wrote. Seattle […] [Read More]
Starbucks says baristas can have tattoos
Break out the tats, baristas. Starbucks has decided that tattoos are OK after all for it store employees. So are, gasp, untucked shirts and nose studs. Even gauges, those large earrings, will be OK. This all came as Starbucks announced a pay raise for "partners," though it didn't specify an amount. The pay raises begin […] [Read More]
Yelm man, Edward Smith, tells Brit TV: I've had sex with 700 cars
A man who says his name is Edward Smith and that he lives in Yelm, Wash., told a British morning TV program on Tuesday that he has had sex with more than 700 cars. That's correct: Not in but with. Monty Python couldn't make this up. Reports ITV's "This Morning:" Since he was 14 years […] [Read More]
No fish story: Angler boats 482-pound halibut
We hope Jack McGuire likes halibut. The Orange County, Calif., fishermen recently pulled a 482-pound halibut into a boat in Glacier Bay, Alaska. The fish was 95 inches long and large enough to produce 225 pounds of fillets, according to ABC News. A record? No, the fish was brought into the boat by harpoon, so […] [Read More]
Louie's Cuisine, Ballard landmark restaurant, closes
Ballard has lost a landmark. Louie's Cuisine of China has announced on its website that Sunday was its last night. It is with a heavy heart that I must share with you the news that Louie's will soon bid an affectionate but reluctant farewell to the many wonderful and loyal customers who have come through […] [Read More]
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This Friday, 29 May sees the tenth International ME Conference put on by UK research charity Invest in ME (IiME) in London. The day-long conference will include 220 participants from 17 countries and will be attended by researchers, clinicians and patients.
Discuss the article on the Forums.
Illness is not our true self/soul
Discussion in 'Spirituality and ME/CFS' started by Gingergrrl, Jul 10, 2014.
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subset: commoncrawl


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I absolutely love this place, worth every $$ spent, the Food fresh and amazing. Excellent customer service, clean, organized.
3 Days in Fort Lauderdale
Museum Guide for Fort Lauderdale
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I LOVE ZONA FRESCA! This is always the place I will go first if I want something really delicious, quick and for a good price in Ft Lauderdale. The restaurant is always clean, the staff are friendly and it's just great fresh food. I think the fish tacos are amazing!
Very fresh mexican food made to order there is take out and dine in.This is one of my favorite places to go for quick mexican that is made to order. it has salsa bar to accompany your food.if there is a line waiting it is worth the wait alot of locals eat here too.
Its not the nicest place but the guac is good, fish tacos are awesome.. really casual place, dress down and eat.
Great made to order mexican fast food with guacamole that is great for sharing and really good! Also in Pompano.
We were into mexican so we asked around. The bell boy at the Hilton advised us to go here. Simple but great food. We had a Burrito with Shrimp and enshelada. Freshly made great ingredients and really big portions. Friendly staff and for those who can;t resist a free goodies bar.... You can sit outside or inside. everything simple but...
It's kind of like a Moes, Chipotle or Qdoba. It wasn't bad, but with all the other great stuff to eat around here, I wouldn't recommend waisting your money here. The rice was bland, the steak was flavorless and stuffed peppers are so so. The shredded beef was good, but I wouldn't recommend getting it enchilada style. It would have...
This place was delicious! Wish there were one in Ohio! Very clean and great price. I loved the salsa bar, anytime I can have all the Pico I want is awesome.
All food fresh prepared as you order. I love the salads, i always eat the garden chicken salad and ask them to put it in a taco bowl. My boyfriend always eats Baja Ranchero which is a full meal of steak or chicken, stuffed peppers, rice beans and guacamole.
I love the freshness and the fact that they don't over season everything! Unlike the bigger chains, they still offer free chips and salsa bar. The salsa is so fresh and good! I love this place and for what you get for the money it's the best hands down!
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Up one level: Canacona


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Built in the 1930's, Miami's Mansion club has plenty of history.
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Paolo Mojo
Walker & Royce
Paolo Mojo is firmly established as a leading light on the underground house music scene with a large global following and a consistent production schedule that keeps him in demand all over the world. With an astonishing work ethic in the studio and his endless special re-edits, he's an innovative DJ whose enviable technical ability and a thriving, huge loyal underground fanbase ensure that he will remain a star of the scene for many years to come.
Part of the Nurvous Records NY crew, Walker & Royce have been packing a serious punch with an array of scorching remixes and a string of club-ready originals. 2012 has been a breakthrough year for them with releases on Crosstown Rebels, Pets Recordings and many more.
Join us as Kontrol presents Paolo Mojo with Walker & Royce and Troncoso at Mansion Nightclub in Miami Beach.
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"The 10 Trends That Are Changing The Face Of The NFL As We Know It"
03 Nov, 2012 by Craig Newmark
Print this article Font size -16+
One of them is "The no huddle is about to take over the league."
We'll see.
Also see...
Father Hands Out Haymakers to Teenage Girl Punks for Attacking His Daughter [Video]Jun 29, 2015 | By Terresa Monroe-Hamilton
This dad punches the crap out of two girls attacking his daughter. Tell me you wouldn't do the same if a pack of thugs were beating on your child. I...Read More
Craig Newmark
Associate Professor of Economics, North Carolina State Univ.
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Well, that must have been a heck of an important call… at the Grand National Cross Country event in Ohio,
How to Make the Track Team in Fundamentally Transformed America (and Get Rich Doing It)
Sports are based on the countermoonbat principle of competition. Unless the game is fixed, the athlete who performs best wins.


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By Cory Edwards on
The Social Media, Mobile Marketing Link[Continue Reading...]
/ Cory Edwards / 5 days ago
Cory Edwards is head of Adobe's Social Business Center of Excellence. He is responsible for integrating social media into the way Adobe does business. Edwards and his team consult and train departments across Adobe to define and measure their social strategies. In addition, they identify opportunities to innovate and pilot new social business programs.


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NBC SportsTalk: Impressions of Yu
Mar 8, 2012, 8:50 AM EDT
I was on NBC SportsTalk last night, talking to Russ about the Yu Darvish debut. If you read my reports from the ballpark you got the general gist. But this time you get it from a parking lot in front of a ballpark while holding a mic, so there's that.This video is no longer available. Click here to watch more NBC Sports videos!
- paperlions - Mar 8, 2012 at 8:58 AM
See….after talking to Michael Young, that double to RF might have been "a smash past the diving Michael Young".
- orton1227 - Mar 8, 2012 at 9:54 AM
On LoneStarBall.com, we have an acronym for that: PADMY (past a diving michael young). It happened so frequently, we had to have a short version.
- cur68 - Mar 8, 2012 at 11:57 AM
I like PADMY. Its right there with TOOTBLAN, IMO.
- randomdigits - Mar 8, 2012 at 9:00 AM
The biggest issue I saw with Yu coming into the league was pitch count and he did nothing yesterday to ease my concerns in that regard.
- paperlions - Mar 8, 2012 at 9:02 AM
- randomdigits - Mar 8, 2012 at 9:45 AM
Nice rebuttal.
I am not denying that there is significant value in getting 6 quality innings from him a start. That will not, however, be enough to move him into the upper echelon of starters.
Look at the game logs:
If he is running those kinds of pitch counts in Japan how can you say it won't be an issue in the states pitching every 5 days instead of every 7?
- paperlions - Mar 8, 2012 at 11:34 AM
What I am saying is that a single 2 inning stint at the beginning of spring training is no basis to change ones opinion. It provides no information whatsoever to address your concern. If he had blown through two innings on 15 pitches, it still would not have been a basis to change opinions.
- randomdigits - Mar 8, 2012 at 12:23 PM
Then you should have said that.
I agree the sample size is way too small to be actually useful. I was simply using the chance to bring up the topic of pitch counts, since no one in the media seems to think it will be a concern.
I know Texas supposedly doesn't keep track of pitch counts but I firmly believe that it will be the single biggest limiting factor on Darvish in the States.
- rohlo - Mar 8, 2012 at 1:35 PM
guy could be good? kinda cocky IMO will veneable of the padres rocked a staright to center 400 ft+++ double off him…only reason it was a double is becasue of hte huge wall to staright center..it would have been out of any ballpark in mlb and he squared up on the hit as well… yu's response- "it was kind of wind aided" LMAO the wind is what kept it from clearing the huge center field wall!! the wind was blowing in as well!! what a douche!! i say "FAHK YU".. cant believe teams would pay so much to negotiate with these overated asian pitchers and hitters.. dice k has been mediocre or injured and foukodome has been average as well!
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Both authors contributed equally to this work.
Cytokinin and gibberellin activate SaMADS A, a gene apparently involved in regulation of the floral transition in Sinapis alba
Article first published online: 25 DEC 2001
DOI: 10.1046/j.1365-313x.2000.00859.x
The Plant Journal
Volume 24, Issue 1, pages 103–111, October 2000
Additional Information
How to Cite
Bonhomme, F., Kurz, B., Melzer, S., Bernier, G. and Jacqmard, A. (2000), Cytokinin and gibberellin activate SaMADS A, a gene apparently involved in regulation of the floral transition in Sinapis alba. The Plant Journal, 24: 103–111. doi: 10.1046/j.1365-313x.2000.00859.x
Publication History
- Issue published online: 25 DEC 2001
- Article first published online: 25 DEC 2001
- Received 19 May 2000; revised 17 July 2000; accepted 19 July 2000.
- Abstract
- Article
- References
- Cited By
- cytokinin;
- gibberellin;
- floral signal;
- SaMADS A;
- Sinapis alba;
- Arabidopsis thaliana.
In plants of Sinapis alba induced to flower by one long day, the MADS box gene, SaMADS A, is expressed initially in the central corpus (L3 cells) of the shoot apical meristem (SAM), about 1.5–2 days before initiation of the first floral meristem. We have combined a physiological approach by testing the effects of three putative floral signals on SaMADS A expression in the SAM of S. alba plants with a transgenic approach using Arabidopsis thaliana plants. A single application of a low dose of a cytokinin or a gibberellin to the apex of vegetative S. alba plants is capable of mimicking perfectly the initial effect of the long day on SaMADS A transcription. A treatment combining the two hormones causes the same activation but seems to enhance the level of SaMADS A expression. A sucrose application to the apex of vegetative plants is, on the contrary, unable to activate SaMADS A expression. None of these chemicals, alone or combined, is capable of causing the floral shift at the SAM. Since the constitutive expression of SaMADS A leads to precocious flowering in A. thaliana and antisense expression of a fragment of the A. thaliana homologue AGL20 leads to a delay in flowering time, these results are consistent with SaMADS A activation being an intermediate event in a cytokinin- and/or gibberellin-triggered signal transduction pathway that is involved in the regulation of floral transition in S. alba.
Plant development is controlled by both environmental and endogenous factors. In photoperiodic plants, exposure of leaves to a day length favourable for flowering results in the production and transmission by these leaves of one or several floral signals, collectively called 'the floral stimulus'. Arrival of these signals at the shoot apical meristem (SAM) causes cessation of leaf formation and start of flower initiation. Molecular and genetic studies have shown that this morphogenetic switch is governed by the activation of interacting groups of genes, including for instance genes such as CO ( Simon et al., 1996 ) and FPF1 expressed early in the switching SAM ( Kania et al., 1997 ) and genes such as LFY and AP1 expressed later and responsible for floral meristem identity ( Ma, 1998; Pidkowich et al., 1999 ). So far, studies attempting to relate the arrival at the SAM of particular floral signals of leaf origin with activation of some of these genes are scarce. This is because the nature of the transmissible floral signals is still a controversial issue ( Bernier et al., 1993 ), and thus the times of arrival at the SAM of these signals are often entirely unknown.
In Arabidopsis thaliana, there is evidence that a gibberellin (GA) is one of the primary floral signals ( Koornneef et al., 1998 ; Levy and Dean, 1998). In this species, it was indeed found that flowering of mutants deficient in GAs is delayed under long days or even suppressed under short days unless GA3 is applied ( Wilson et al., 1992 ). Activation of GA-mediated signal transduction pathways, such as in plants with a mutation in SPY or over-expressing FPF1, leads to early flowering and other phenotypic changes that mimic the effects of GA application ( Jacobsen and Olszewski, 1993 ; Kania et al., 1997 ). Moreover, exogenous or endogenous GAs activate in the SAM of A. thaliana the expression of LFY, one of the major genes responsible for floral meristem identity ( Blázquez et al., 1998 ), through an identified cis element in the LFY promoter that resembles the consensus binding site for some MYB transcription factors ( Blázquez and Weigel, 2000). Up-regulation of LtGAMYB in the shoot apex of Lolium temulentum at floral transition is also believed to be caused by a GA signal originating in leaves ( Gocal et al., 1999 ).
Another floral signal in A. thaliana is sucrose, as shown by observations that the floral response to an inductive photoperiodic treatment is severely impaired in conditions where leaves are unable to provide an extra amount of sucrose to the SAM ( Corbesier et al., 1998 ). Sucrose can hasten considerably the flowering time of two late cold-requiring ecotypes and several late-flowering mutants ( Roldán et al., 1999 ), and it has been shown that sucrose acts in concert with a GA to activate LFY expression ( Blázquez et al., 1998 ).
In Sinapis alba, a long-day (LD) plant that can be induced to flower by exposure to a single long day, the timing of movement of the floral stimulus from leaves to SAM has been determined ( Bernier, 1989), and two putative floral signals, sucrose and a cytokinin, have been identified ( Bernier et al., 1993 ). For each of these signals, the timing of their increased flux from induced leaves to the SAM was determined ( Lejeune et al., 1993 ; Lejeune et al., 1994 ). Also, although the application to plants kept under non-inductive short days (SDs) of either sucrose or a cytokinin fails to cause flowering, each of these compounds causes several cellular and metabolic events in the SAM that are typically observed after floral induction by one long day ( Bernier et al., 1993 ). Whether a GA is also a floral signal in S. alba remains an unresolved question. Application of a GA under SDs or of a GA biosynthesis inhibitor under LDs yielded inconclusive results ( Bernier, 1969). However, FPF1, a gene involved in a GA-mediated signal transduction pathway, is expressed early in the S. alba SAM in response to floral induction ( Kania et al., 1997 ), suggesting that increased GA sensing is a component of the floral transition process in this species, as in A. thaliana (see above).
We have started to investigate the effects of these three putative floral signals on the transcription of genes involved in the SAM floral transition in S. alba. Genes expressed early during this transition are presumably the best candidates as targets for individual floral signals. One such gene, SaMADS A, was identified as being expressed in the SAM of S. alba well before initiation of the first floral meristem ( Menzel et al., 1996 ). SaMADS A protein presents a high degree of similarity to AGL20 (now renamed SOC1, Samach et al., 2000 ) of A. thaliana, with 100% similarity between the MADS box domains and 95.3% similarity between the K box domains. In this paper, we show that the constitutive expression of SaMADS A in A. thaliana leads to precocious flowering under SD and LD conditions and that the constitutive expression of an AGL20 fragment in the antisense orientation leads to a delay in flowering. Recent work, published after submission of this paper, has shown that AGL20, which is a target gene of CO, has a major role in promoting flowering in A. thaliana ( Onouchi et al., 2000 ; Samach et al., 2000 ). AGL20 was proposed by Samach et al. (2000) to be part of a transcriptional cascade within the SAM that results in activation of LFY and other floral meristem identity genes. In the present paper, we also report that a single cytokinin or gibberellin application, but not a sucrose application, does indeed activate the transcription of SaMADS A in the SAM of S. alba plants kept under non-inductive SD conditions, in a way that mimics perfectly the initial activation caused by an inductive LD. It is suggested that SaMADS A acts within a signal transduction pathway involved in the SAM floral transition and activated by a cytokinin and/or a gibberellin.
Transcription of SaMADS A in S. alba in response to floral induction caused by one long day
The work of Menzel et al. (1996) showed early activation of SaMADS A in the SAM of S. alba plants induced to flower by continuous 16 h LDs. Since the timing of movement of the floral stimulus and putative floral signals of S. alba were determined in an inductive system involving a single 22 h LD, it was first necessary to establish the spatial and temporal transcription of this gene in this particular inductive system.
In situ hybridizations were performed with longitudinal sections of apical buds of LD-induced plants collected from 24 to 72 h after the start of the LD, using 35S-labelled SaMADS A antisense RNA as a probe. SaMADS A was not expressed in the SAM of vegetative plants continuously grown under SD conditions ( Figure 1a). The first transcription of SaMADS A was detected in the SAM of some, but not all, plants 24 h after start of the LD, i.e. 16 h after the start of the photo-extension period of this LD; transcription was present in all plants collected at 32 h, that is about 1.5–2 days before initiation of the first floral meristem which occurs in most plants at 66–80 h after start of the LD ( Figure 1b). As described by Menzel et al. (1996) , transcription was initially localized to the central zone only. In fact, we observed that transcription started in a few cells deeply localized in the central corpus (just above the rib meristem) and then spread to the whole central zone, except the superficial two layers (L1 and L2) and also the outermost layer of L3 in some SAMs ( Figure 1b). At 56 h, about 0.5–1 day before initiation of the first floral meristem, RNA transcripts were more abundant in the central zone (although still excluded from L1 and L2 but no longer from the outermost layer of L3) and were now seen in the cells of the rib meristem just below the central zone ( Figure 1c). There was clearly no transcription in the peripheral zone. At 72 h, when the first floral meristem was emerging from one flank of the inflorescence meristem, the hybridization signal had spread, although at a reduced level, to the peripheral zone ( Figure 1d), as previously observed by Menzel et al. (1996) . Again we found that transcription was absent in the L1 and L2 cell layers of both the central and peripheral zones ( Figure 1d). There was no transcription in nascent floral meristems (left flank of the SAM in Figure 1d). In control experiments with the SaMADS A sense probe, there was no hybridization signal above background level ( Figure 1e).
Transcription of SaMADS A in S. alba caused by a cytokinin or a gibberellin under non-inductive SD conditions
Two-month-old plants, kept vegetative by growth under continuous SDs , were treated once with solutions of the three putative floral signals applied directly to the apical bud. Solution concentrations were 2% for sucrose (SUC), and 5 × 10−5 m for the cytokinin benzylaminopurine (BAP) and for gibberellin A3 (GA3). The concentrations of applied solutions were chosen on the basis of previous studies ( Bernier et al., 1990 ; F. Bonhomme et al., unpublished data) . Times of application were derived from previous knowledge about the timings of movement of the floral signals in S. alba plants induced by one long day. SUC levels started to increase in the phloem sap, reaching the apical bud and the SAM itself extremely early during the LD, in fact at 10 h after its start ( Bodson and Outlaw, 1985; Lejeune et al., 1993 ). SUC was apparently a fast-moving component of the floral stimulus since the slowest components moved from the leaves to apex only 8–10 h later, as indicated by defoliation experiments ( Bernier, 1989). Accordingly, and taking into account that there is a lag period of 4–5 h between application of a chemical and its arrival at the SAM (see Experimental procedures), the times of solution application to the bud were 6 h after the start of the experimental SD for SUC and 16 h after the start of this SD for BAP and GA3. None of the applied compounds caused flowering. SaMADS A transcription was investigated at 16 (SUC only), 24, 32, 48, 56 and 72 h after the start of the experimental SD.
There were no SaMADS A transcripts detectable in the SAM of SUC-treated plants throughout the investigated period ( Figure 1f).
SaMADS A transcripts were first detectable in the SAM of all BAP-treated plants 8 h after the application of the chemical, i.e. 24 h after start of the experimental SD ( Figure 1g). Transcripts were localized in the central zone, except in L1, L2 and the outermost layer of L3, as well as in the cells of the rib meristem just below the central zone ( Figure 1g). Transcript localization was identical to that initially observed above in the SAM of LD-induced plants ( Figure 1b). Despite there being only a single BAP application, the signal was maintained in the central zone and rib meristem for at least up to 56 h after the application, i.e. 72 h after start of the experimental SD, but unlike in LD-induced plants it did not spread with time to the peripheral zone ( Figure 1h).
The effect of a GA3 application was identical to that of a BAP application: activation of SaMADS A transcription started 8 h after the GA3 treatment and was observed in exactly the same tissue ( Figure 1i). As for BAP, SaMADS A activation caused by GA3 was also long-lasting, and did not spread with time to the peripheral zone ( Figure 1j).
Cytokinin and gibberellin
As BAP and GA3 were capable of activating SaMADS A expression independently of each other, it was decided to analyse the effect of a combination of these two compounds. A mixture of 5 × 10−5 m BAP and 5 × 10−5 m GA3 was applied once to the apical bud of SD-grown plants 16 h after the start of the experimental SD. The combined treatment caused an activation of SaMADS A transcription with the same timing and localization as those activations produced by the individual treatments, but the hybridization signal was somewhat stronger, indicating that the two hormones possibly had additive effects ( Figure 1k, compare with Figure 1g,i).
Modulation of the time to flowering in transgenic A. thaliana plants
A. thaliana is a facultative long-day plant, flowering earlier under long-day than under short-day conditions ( Koornneef et al., 1998 ; Levy and Dean, 1998; Piñeiro and Coupland, 1998). To analyse whether the SaMADS A gene from S. alba and the homologous gene from A. thaliana are involved in the control of the floral transition, we have generated transgenic A. thaliana plants that over-express the coding region of SaMADS A in the sense orientation or an AGL20 fragment in the antisense orientation under the control of the 35S CaMV promoter. We obtained six kanamycin-resistant lines with the SaMADS A coding region in the sense orientation and have selected 88 transgenic Arabidopsis lines with an AGL20 antisense construct.
Expression of the SaMADS A transgenes was monitored by Northern blotting. As shown in Figure 2, all over-expressing lines showed expression of the transgene; however, lines S1–S3 showed only weak expression compared to the RNA level of the transgene in lines S4–S6. In wild-type plants, no hybridization could be observed ( Figure 2). To analyse phenotypic changes in SaMADS A over-expressing plants, these were grown in parallel with wild-type plants in the same environment. Seedlings at the cotyledon stage appeared normal, and germination and development up to the four-leaf stage showed the same duration as in wild-type plants. From this stage onwards, the duration of the following phases in some lines was remarkably diminished, which led to precocious bolting and earlier flowering ( Figure 3). The transgenic lines S1–S3, which showed only weak expression of the SaMADS A transgene, did not flower earlier than wild-type plants under LD conditions ( Figure 4), whereas the lines S4–S6 showed a significant reduction of the vegetative phase under these conditions. Under SD conditions, the time to flowering was also remarkably reduced in lines S1–S3, but was even shorter in lines S4–S6 ( Figure 4). The transgenic line S6 showed the most dramatic reduction of the vegetative phase. The time to flowering was reduced to 52 days, compared to 86 days in wild-type plants under SD conditions. The decrease in leaf number by about 50% is mainly caused by a decrease of rosette leaves, and the number of cauline leaves is only slightly reduced.
Most of the 88 AGL20 antisense lines showed a slight delay in flowering, whereas several lines showed a markedly late flowering time compared to wild-type plants both under SD and LD conditions. From the late-flowering lines, eight were selected and further analysed. From homozygous kanamycin-resistant plants, the amount of RNA from the antisense transgenes was determined by Northern blotting. Very strong expression of the antisense transgene was seen in five lines (A10, A11, A25, A40 and A 75) and very low expression in the other selected lines (data not shown). With the exception of line A75, the lines with the strongest expression of the antisense construct were the lines that flowered latest under LD conditions, with line A40 flowering 13 days later than the wild-type plants ( Figure 4). However, under SD conditions, the flowering time was not strictly correlated with the transgene expression. Under these conditions, line A40 was also the latest line to flower, flowering 17 days later than the wild-type plants. Floral development was not affected in the transgenic plants: flowers developed normally, were fertile and the number of seeds per silique was not altered.
Evidence that SaMADS A is involved in flowering time control
SaMADS A and SaMADS B are the earliest up-regulated MADS box genes from S. alba that are expressed in the SAM upon floral induction ( Menzel et al., 1996 ). The homologous genes to SaMADS A and B from A. thaliana, AGL20 and AGL8, show a similar pattern of expression in A. thaliana (Borner et al., unpublished results ; Mandel and Yanofsky, 1995). In this paper, we have shown that the over-expression of SaMADS A in A. thaliana is sufficient to shorten the vegetative phase of transgenic plants. More direct evidence that the gene is involved in flowering time control comes from transgenic approaches with an antisense construct of the homologous gene AGL20 in A. thaliana, which leads to late-flowering phenotypes. Moreover, we have shown that the over-expression of AGL20 also causes very early flowering in transgenic A. thaliana plants, confirming similar data from Samach et al. (2000) . We have also found that transposon-induced mutations in AGL20 cause late flowering in null mutants (Borner et al., unpublished results) . Therefore, the results with S. alba and A. thaliana are consistent with the assumption that SaMADS A and AGL20 play similar and important roles in the floral transition in these two species.
Transcription of SaMADS A in S. alba in response to floral induction caused by one long day
Our analysis of SaMADS A gene transcription during the floral transition caused in S. alba by one long day has generally confirmed the observations of Menzel et al. (1996) . Thus, activation of SaMADS A occurs well before initiation of the first floral meristem, and this activation is initially restricted to the central zone of the SAM. It spreads to the rib meristem and peripheral zone only at later times. A distinctive observation of our study is that the activation of SaMADS A occurs only in L3 cells, not in the L1 and L2 cells of the SAM. This was not reported by Menzel et al. (1996) , perhaps because they induced their plants to flower by continuous LDs , i.e. an inductive treatment much stronger than our single LD. Indeed, in plants of S. alba induced by successive LDs, we have observed that activation of this gene eventually occurs in L1 and L2 cells (data not shown). However, our results show that the floral transition can proceed when SaMADS A is activated only in L3 cells.
The initial activation of SaMADS A in the central L3 cells or corpus and its later activation in other zones of the SAM are interesting. The central corpus is known to consist of relatively undifferentiated cells, including a small number of self-maintaining pluripotent stem (initial) cells ( Evans and Barton, 1997). These cells replenish continuously both the peripheral zone, which produces the leaf or flower primordia, and the rib meristem, which gives rise to the pith of the shoot axis. It is tempting to speculate that SaMADS A could initially switch on at least part of the floral morphogenetic programme in the undifferentiated cells of the central corpus and then this new programme could extend progressively to the surrounding zones through cell divisions.
Transcription of SaMADS A in S. alba caused by a cytokinin or a gibberellin under non-inductive SD conditions
SaMADS A activation under non-inductive SD conditions may be achieved by applying to the apex a single low dose of a cytokinin, a GA or a combination of these two hormones, but not by a sucrose application. Activation by the cytokinin or the GA is restricted to the central L3 cells (corpus) and rib meristem cells, just as in the initial steps of activation in LD-induced plants. Activation is first detectable in all cases 8 h after hormone application, and since the application was made 16 h after the start of the experimental SD, activation of SaMADS A is first detectable at 24 h after the start of the experimental SD. Activation of SaMADS A expression in LD-induced plants also started 24 h after the start of the LD. Thus, relative to the start of the light period, the timings of activation caused by the LD or the hormones are identical. Thus, both spatially and temporally, each hormone is capable of mimicking almost perfectly the initial effect of the LD on SaMADS A transcription. Interestingly, when the two hormones are applied together, the localization and timing of SaMADS A activation remain unchanged but activation is apparently greater than after application of the individual hormones. These observations are consistent with SaMADS A activation being an intermediate event in a signal transduction pathway activated by a cytokinin and/or a GA and involved in the floral transition of the S. alba SAM.
Activation of SaMADS A is consistently detectable 8 h after hormone applications. As it takes compounds applied to the apical bud 4–5 h to reach the SAM itself (see Experimental procedures), we infer that SaMADS A transcription is activated within 3–4 h following arrival of the hormones at the SAM. Similar time ranges have been reported for activation of other genes by cytokinins or GAs ( Davies, 1995; Schmülling et al., 1997 ).
Despite there being only a single application of the hormones, the activation of SaMADS A is long-lasting, persisting for at least 56 h after application. The late spreading of this activation to the peripheral zone of the SAM, observed after one LD, does not occur after the hormone applications under SD conditions, and this might be one of the reasons why these hormones do not cause the floral transition under SD conditions . Presumably, the late spreading process under LD conditions is dependent on the activation (or deactivation) of other genes.
The above results strongly support the idea that a cytokinin and a GA are floral signals in S. alba. As far as cytokinins are concerned, previous analyses of (a) the changes in their endogenous levels and fluxes in plants induced by a long day, and (b) the effects of their application to the bud of non-induced plants, have provided strong evidence that this hormone family participates in the control of the floral transition in this species (summarized in Bernier et al., 1993 ). Dewitte et al. (1999) have reported that the cytokinin levels in the SAM of tobacco decrease markedly at the floral transition. This result does not disprove the above conclusion concerning S. alba as it is well known, from both in vivo and in vitro studies, that if flowering is to occur, there is a permissive range of cytokinin concentrations in many species, with too low or too high concentrations being inhibitory depending on the plant species and age and prevailing growing conditions (reviewed in Bernier et al., 1981 ; Bernier, 1988). Thus, the direction of changes in cytokinin levels at floral transition may be opposite in different experimental systems.
In the case of the GAs, evidence for their participation in the control of the floral transition in S. alba is based on the observation of Kania et al. (1997) that FPF1, a gene involved in a GA-dependent signalling pathway, is activated in the SAM at an early step of the floral transition, well in advance of initiation of the first floral meristem. Thus, GA sensing appears to be implicated in the SAM transition in S. alba, just as in A. thaliana (see Introduction). In A. thaliana, an increased GA level is a critical factor in the control of the floral transition ( Blázquez and Weigel, 2000; Blázquez et al., 1998 ; Levy and Dean, 1998). Whether a similar situation prevails in S. alba is unknown as the levels and fluxes of GAs at floral transition in this species have not yet been investigated.
Interestingly, SaMADS A transcription is activated by two different kinds of hormones, and the effects of these hormones are apparently additive. This is a situation frequently encountered in the field of plant hormones which are known to substitute for each other in many cases and also often act in concert ( Crowell and Amasino, 1994; Davies, 1995; Izhaki et al., 1996 ). As far as the expression of a gene such as SaMADS A is concerned, a possibility is that the transduction pathways triggered by the two hormones partially overlap. This may happen when a pathway crosses another at some point ( Genoud and Métraux, 1999) or when one hormone is capable of altering the level of the other hormone ( Davies, 1995). Alternatively, the transduction pathways may be independent but finally affect the same target gene.
Experimental procedures
S. alba plant materials
Plants of S. alba were kept vegetative by growth under continuous 8 h SDs (light period: 08:30–16:30 h) as described by Lejeune et al. (1988) . The irradiance at the top of the plants was 150 µmol m−2 sec−1 and was provided by very-high-output Sylvania fluorescent white tubes (Zaventem, Belgium). When the plants were 2 months old, they were exposed to the following treatments:
- 1maintained under SD conditions;
- 2induced to flower by exposure to one 22 h LD (08:30–06:30 h) and then returned to SD conditions;
- 3maintained under SD conditions and treated once with an aqueous solution of 2% sucrose (SUC) applied directly to the apical bud at 14.30 h, i.e. 6 h after the start of the experimental SD;
- 4maintained under SD conditions and treated once with an aqueous solution of 5 × 10−5 m benzylaminopurine (BAP) (Sigma B-5898) applied directly to the apical bud 16 h after the start of the experimental SD, i.e. at 00:30 h;
- 5maintained under SD conditions and treated once with an aqueous solution of 5 × 10−5 m gibberellin A3 (GA3) (Sigma G-7645) applied directly to the apical bud 16 h after the start of the experimental SD, i.e. at 00:30 h; or
- 6maintained under SD conditions and receiving a combination of the two previous treatments.
Time was computed from the start of the experimental SD or LD. All experiments were repeated 2–4 times. Apical buds were collected at various time intervals after the start of the experimental SD or LD. Aqueous solutions of the chemicals were applied to a small cotton plug inserted between the young leaves of the apical bud. An amount of about 0.3 ml was applied to each bud and 0.1% Tween-20 was added to all solutions. Tween-20 did not affect floral induction ( Gonthier, 1987). Experiments using 3H-thymidine or red eosine applied in the same way allowed estimation of the lapse of time required for an applied chemical to reach the SAM itself (4–5 h) ( Gonthier, 1987).
In situ hybridization
Shoot apical buds of S. alba plants were fixed in 2% formaldehyde in 100 m m phosphate buffer, pH 7.2 (16 h at 4°C). Fixed tissues were dehydrated and embedded in paraffin according to the procedure described by Bonhomme et al. (1997) . Longitudinal sections (8 µm) were mounted onto poly- l-lysine-coated slides. 35S-UTP-labelled antisense and sense riboprobes were synthesized from truncated cDNA without the MADS box and poly(A) tail, and inserted in the SmaI site of pBS SKII+ (Stratagene), using T3 or T7 RNA polymerase according to the manufacturer's instructions (Promega, Madison, WI, USA). The labelled RNA probes were hydrolysed to give fragments with an average length of 150 nucleotides. Pre-treatments, hybridizations and washes were performed as previously described ( Bonhomme et al., 1997 ). Slides were exposed for 8 weeks. Four to eight SAMs (from two to four independent experiments) were examined for each experimental time point.
Northern blot
RNA was isolated from two- to four-leaf stage seedlings of A. thaliana ground in liquid nitrogen and stored at −80°C according to Kania et al. (1997) . For Northern blot analyses, 20 µg RNA per sample were separated on an agarose gel containing formaldehyde. After blotting to a GeneScreen membrane (NEN, Boston, MA, USA), the RNA was fixed to the membrane by baking at 80°C under vacuum for 1 h.
Hybridization was performed overnight at 65–68°C in 1.5 × SSPE, 7% SDS, 10% PEG 8000, 100 µg ml−1 salmon sperm DNA and 250 µg ml−1 heparin, using an SaMADS A cDNA fragment without the MADS box as a probe ( Menzel et al., 1996 ). Filters were washed with 0.1 × SSC/0.1% SDS and exposed to Kodak X-Omat AR or BioMax MR-1 films (Eastman Kodak, Rochester, NY , USA) for 5 days.
Transgenic approaches in A. thaliana
For over-expression of the coding region of SaMADS A in A. thaliana under the control of the 35S CaMV promoter, the EcoRI fragment of the SaMADS A cDNA ( Menzel et al., 1996 ) was inserted into the pRT104 vector ( Töpfer et al., 1987 ) and transformed into E. coli DH5α cells. After partial digestion with HindIII, a fragment containing the expression cassette was isolated and inserted into the BIN19 vector ( Bevan, 1984). The recombinant vector pBIN19–SaMADS A was transformed into the Agrobacterium tumefaciens strain C58C1 by a standard transformation procedure ( Höfgen and Willmitzer, 1988). The obtained recombinant bacteria were used to transform A. thaliana ecotype Columbia by in planta transformation ( Bechtold et al., 1993 ). After in planta transformation, plants were grown in a growth chamber under LD conditions. Plant seeds were harvested 5 weeks after infiltration, sterilized, and plated on germination medium containing 500 mg l−1 timenten to suppress growth of Agrobacterium and 50 mg l−1 kanamycin for selection of transformants. Kanamycin-resistant transformants were transferred to soil, seeds were harvested, and homozygous lines were selected with kanamycin.
For the antisense approach, an EcoRI fragment of an AGL20 cDNA which does not include the MADS box (Borner et al., unpublished results) , was cloned in the pRT104 vector in antisense orientation and further treated as described for the SaMADS A over-expression.
For analysis of the transgenic lines, seeds were sown on soil, singled out in lawn soil, and grown in climate chambers at 20°C under fluorescent tubes emitting a photon flux density of 160 µmol m−2 sec−1. Illumination time was 8 h for SD conditions and 16 h for LD conditions. The time to flowering was measured in days until the opening of the first flower and by total leaf number .
We thank H. Sommer, R.F. Lyndon, F. Cremer and J. Chandler for critical reading of the manuscript, and A. Pieltain and N. Detry for technical assistance. This work was supported by a grant from the Fonds Spéciaux (1994–1997) of the University of Liège and Pôles d'Attraction Interuniversitaires Belges (Service du Premier Ministre, Services Fédéraux des Affaires Scientifiques, Techniques et Culturelles, P4/15). F.B. was a research assistant of the 'Fonds National de la Recherche Scientifique' of Belgium during part of this work.
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text:
jack russel terrier
hi im Gobby. i am very loving and excitable im about 2 years old. im a good boy when left alone and at night. I DONT like the postman and like barking and jumping up at the letter box, i also dont like anyone near my food bowl, i am fine if yo
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The Price Is Right "Live" Show Coming To Amarillo
Get ready Amarillo here it comes...The Price Is Right game show is on its way to you and you don't want to miss it!
Get ready Amarillo here it comes...The Price Is Right game show is on its way to you and you don't want to miss it!
This morning we found ourselves in a situation that we didn't know if we could get out of. Everyday after the show we enjoy getting a healthy shake for the day. Well, today everyone parked in the back parking lot of the radio-station and which caused our vehicle to be blocked in
This is one of our favorite games we love to play on the morning show and try to win cool prizes for our listeners. The game is called Radio Pop Challenge and the way we play it is Angel Dee will play a snippet of a song and we have to buzz in and guess the correct song with title and artist. The
Things have been getting heated the 'Dash for Cash' scavenger hunt. People are getting excited and anxious to find our cash.
We are so excited to bring 'Dash for Cash' back!
Here's some phrases that only people in the panhandle will know and out of towners will have no clue what your talking about.
Every Monday on 'The Hacker Morning Show' we play a game called Radio Pop Challenge. The way it works is one person the show will pick 8 songs for the other show members to try to guess what song it by hearing a small clip from the song selected. The
Apparently this paintball player was caught up in the moment for him not to see a metal barrel in front of him as he runs into nearly knocking him out. He hit the barrel so hard that it puck him backwards on his butt! He couldn't continue the game and had to get himself checked out.This
Alex Tillinghas a college student from Drake not only scores on a half-court show but also scores himself a brand new 2014 Ford F-150. Alex had 30 seconds to make a free-throw, 3 pointer and half-court shot and the truck was his. Wit
Pulling off a on-side kick is really hard but this highschool football team made it look way too easy! By the looks at it they practiced really hard to accomplish this...
Credit: MaxPreps
Sixth grader, Demias Jimerson, who plays in the Arkansas' Wilson Intermediate Football League has been punished for scoring too many touchdowns.


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Garden Botanika, Body Lotion, Almond Cream, 16.9 fl oz (500 ml) (Discontinued Item)
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Garden Botanika, Body Lotion, Almond Cream, 16.9 fl oz (500 ml) (Discontinued Item)
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Garden Botanika is always cruelty-free! We do not test on animals.


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Tags: Asphalt Institute
Asphalt Pavement Alliance names executive director
The Asphalt Pavement Alliance, a partnership of the Asphalt Institute, the National Asphalt Pavement Association, and the State Asphalt Pavement Associations, today named Mike Kvach as its first full-time executive director.
The appointment takes effect immediately. Kvach is charged with mobilizing ... Read More...
Read More...
International Slurry Surfacing Association, Asphalt Institute offering pavement preservation webinars
The International Slurry Surfacing Association (ISSA) and the Asphalt Institute are partnering to offer a second series of 90-minute webinars designed to educate public agencies about different pavement preservation topics. The webinars will take place the first three Thursdays in ... Read More...
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Ruby on Rails Developer
Russia - Last active: 1 month ago - Tests: 2
I've been working with web development projects for 3 years. I had some experince with Ruby on Rails. Now my main development stack is ASP.NET and KnockoutJs and I prefer practicing BDD/TDD approach. I have another job outside the Upwork, so I prefer part-time job.
Ruby on Rails Developer
Spain - Last active: 5 days ago - Tests: 2
Hi, I'm David Ramirez, a freelance Ruby/Rails developer. I've been developing Ruby on Rails applications since 2006, since 2007 as freelance.
Ruby on Rails Expert
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Upwork's Top Rated Developer looking to contribute skills and hard work in RoR projects. Upto now, i worked many RoR applications including some with finest rails architecture written by contributors of Rails community.
Russia - Last active: 2 months ago - Tests: 5
Konstantin is a proficient backend Java/C++ developer with more than 5 years of experience. He is fluent with MySQL and MongoDB databases. Konstantin also works with JavaScript, CoffeScript, jQuery. HTML and CSS. He is initiative and likes a new challenge. Konstantin knows how to pick the right instrument to reach the goal, always meets client's deadlines and writes solid, testable code. He is strong communicator and good team member which is important for an effective collaboration.
Associated with: Datacrafts
Ruby on Rails Developer
Pakistan - Last active: 12/31/2014 - Tests: 1 - Portfolio: 14
I have been working as a web developer since 2000, on both PHP & .NET and more recently Ruby on Rails (commercially since early 2007). Along these years I've delivered training on things like Robotics, Software Engineering, JAVA, PHP and Ruby on Rails. I have also participated in several workshops and conferences talking about Ruby on Rails, plug-in and games programming, XML processing, CSS frameworks or agile development. The list of companies I've worked for includes Shutl, CoffeSender, BookingBug and VeriQual I'm proud to have helped clients ranging from small start ups to big banks and corporations in Spain, Italy, the US and the UK. ------ Experience 8 years PHP development 7 years .NET development 6 years Ruby on Rails experience, most recently working for London-based technology start-up http://shutl.com/uk/ ------ Skills Web: HTML/CSS, JavaScript, JQuery, JSON, Ruby on Rails. Testing: RSpec, Cucumber Deployment: Capistrano, Jenkins, Git Databases: PostgreSQL
- Java
- Ruby
- Git
- Apache Subversion (SVN)
- Scrum
- Agile software developmennt
- Trac
- MySQL Administration
- Ruby on Rails
Associated with: VeriTech
Ruby on Rails Developer
India - Last active: 1 month ago - Tests: 1 - Portfolio: 1
Ruby and Erlang developer
Russia - Last active: 11/16/2014 - Tests: 2
Competent Ruby, Erlang and C developer with over 10 years experience in software development. Was participating in developing a numbers of robust systems, including banner system capable to serve over 400'000 requests per second. Keywords: Ruby, Erlang, C, PostgreSQL, Linux.
Senior Ruby / Rails Developer
India - Last active: 1 month ago - Tests: 1
Available for fixed price as well as hourly engagement ------------------------------------------------------------------------------- Expertise in Ruby, Rails 2 and Rails 3 Version control with Git, Subversion Cloud server deployment to Heroku, Amazon AWS Testing using Rspec, FakeWeb, FactoryGirl, WebMock, Capybara, VCR Backend databases using Redis, PostgreSQL, Memcache, Mongo Authentication gems Devise, Authlogic, CanCan, OmniAuth Message queuing usin Resque, AMQP RVM to deploy multiple rails installations Utilizing existing rails gems for efficient rails development : Rails Performance Management (RPM), will_paginate for pagination Continuous integration using Capistrano Project management using basecamp, pivotaltracker, assembla, jira Well aquainted with agile development and been an active participant of scrum teams HTML (XHTML, W3C Validated) Web services ( SOAP , XML , REST , JSON ) Mobile Applications : Android, iPhone, phoneGAP, Titanium Payment Processors : Paypal, Mercury Pay, WePay Experience with APIs such as : Google Maps API, Twilio API, Facebook, LinkedIn API, Twitter API and various oAuth based APIs
- Ruby on Rails
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Ruby software developer
Serbia - Last active: 21 days ago
I'm passionate RubyOnRails developer who constantly learning new things in this area and I'm trying to keep my code cleaner and clearer. I enjoy in challenges and their solving .
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India - Last active: 1 month ago - Tests: 2
5+ years experience in ruby on rails. Server admin and good in javascript libraries. TDD, BDD, good database skills.


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Seattle Animal Shelter hosts "Cool City Pets" Saturday, May 16
Have you been thinking about adding a pet to your family? Perhaps you don't have the space for a dog or maybe someone in your household is allergic to cats. The Seattle Animal Shelter has the perfect solution: start small. Come on down to the shelter on Saturday, May 16, for Cool City Pets, an […][More]
Posted: May 13th, 2015 under Other Critters, Press Release.


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By Lawrence Van Gelder
Published: February 26, 2004
JANET JACKSON LOSES A ROLE -- Janet Jackson's Super Bowl breast-baring has reportedly cost her a role as the singer and actress Lena Horne, left, in a planned ABC television movie. Reuters reported that Ms. Jackson bowed out of the biographical film after Ms. Horne refused her cooperation in the production as long as Ms. Jackson remained in the role. Stephen Huvane, a spokesman for Ms. Jackson, said she was no longer part of the project ''because Lena didn't want her in it.'' The executive producers Craig Zadan and Neil Meron quit in support of Ms. Jackson. ''ABC and the producers were supporting Janet,'' Mr. Huvane said, ''and Janet realized this wasn't going to change, so she needed to bow out, and then the producers bowed out with her.'' A spokesman for ABC said the television movie was currently ''in limbo.''
JUSTIN TIMBERLAKE CAUSES A FLAP -- Justin Timberlake, who ripped open Janet Jackson's costume to expose her breast at the Super Bowl, was in the middle of a controversy himself yesterday after he canceled his commitment to be the co-host, with Lionel Richie, of ABC's ''Motown 45'' special. The announcement came after a national e-mail, fax and telephone campaign by a coalition of African-American organizations protesting Mr. Timberlake's involvement in the two-hour tribute to Motown, which is to be taped on April 4 at the Shrine Auditorium in Los Angeles and broadcast in May, said a Billboard-Reuters report. ''The selection of Timberlake as co-host of this Motown special is a cultural insult to the black community,'' Najee Ali, executive director of Project Islamic H.O.P.E., said in a statement last week. ''This special, celebrating the success of the legendary music label, should not be compromised in the pursuit of a crossover audience.'' Jeff Margolis, executive producer of ''Motown 45,'' said in a statement that Mr. Timberlake had ''gladly accepted'' when asked to be a host but had to withdraw from all television appearances through April because of a previous commitment to a feature film. Mr. Timberlake is to star with Morgan Freeman, Kevin Spacey and LL Cool J in ''Edison''; he will play a journalist who finds police corruption.
BUDAPEST: TV'S NAKED RUNNER -- Literally outstripping Ms. Jackson, Anettka Feher, a 30-ish Hungarian television host wearing nothing but some jewelry, sat atop a table, legs crossed, yesterday and announced that she was a candidate for a seat in the European Parliament. Hungary joins the European Union on May 1 and has been allotted 24 seats. Ms. Feher, a candidate of the upstart Union Party, rejected any comparisons between herself and Ilona Staller, a former pornography star who is Italian but of Hungarian descent, Reuters reported. Ms. Staller, known as Cicciolina, bared her breasts during a campaign that won her a seat in the Italian parliament in 1987. ''If they think I am just another Cicciolina, they are in for a surprise,'' said Ms. Feher, referring to Hungarian mainstream politicians. ''I am smart, and I have a daily four-hour presence on a national television channel.''
MARIAH CAREY ON TOUR -- Wearing a two-piece silver belly dancing costume, Mariah Carey, left, gave her first concert in the Arab world on Tuesday when she appeared before 5,000 rapturous fans in the heart of Beirut, Lebanon, Agence France-Presse reported. Most of the audience who came to hear her promote her latest album, ''Charmbracelet,'' consisted of young fans in jeans and T-shirts, but the crowd also included Andrée Lahoud, the wife of President Émile Lahoud, and the United States ambassador to Lebanon, Vincent Battle. Ms. Carey is to perform tonight in Dubai.
PRINCE PLANS A TOUR -- For the first time in six years Prince will return to arena stages, singing hits like ''When Doves Cry'' and ''Little Red Corvette'' in a national tour to begin on March 29 in Los Angeles and extend to at least 38 cities, The Associated Press reported. ''It's older music, but it's going to be played in a newer way,'' Prince said in Los Angeles before a half-hour concert with his eight-piece New Power Generation Band.
THEATER: TOGETHER AGAIN -- The Phantom and his true muse are reuniting. Michael Crawford, who starred in the title role of the long-running musical ''Phantom of the Opera,'' will be rejoining its creator, Andrew Lloyd Webber, when Lord Lloyd Webber's semi-operatic musical ''The Woman in White'' opens in May at the Palace Theater in London, The Guardian reported. The show, replacing ''Les Misérables,'' which has moved after a 19-year run at the Palace, is based on the Wilkie Collins Victorian thriller, but this time Mr. Crawford, now 62, will not play the romantic hero but the sinister and devious Count Fosco. Lord Lloyd Webber said, ''We wanted an actor who will take the heart by the throat.''
Wallace Chappell will become the executive director of the Paul Taylor Dance Company, effective March 8. For the past two and a half years Mr. Chappell has been the director of strategic initiatives for American Ballet Theater. . . . Scratch ''Duet'' from the spring lineup of shows on Broadway. This Otho Eskin play, starring Laura Esterman as Sarah Bernhardt and Pamela Payton-Wright as Eleonora Duse, which opened off Broadway on Dec. 4, had been scheduled to open in revised form in early April at Circle in the Square. But Ludovica Villar-Hauser, the play's producer and director, said delays in completing financing for the Broadway production mandated a postponement until the summer or fall.
Photos: Andrew Lloyd Webber, left, is on a team again with Michael Crawford, former Phantom of the Opera. Mr. Crawford and Maria Friedman, shown here at the Palace Theater in London, are to star in Lord LLoyd Webber's ''Woman in White.'' (Report, below right.) (Photo by European Pressphoto Agency)


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shenning (Sarah Henning)
Last login: Sunday, January 4, 2015
Comment history
Funding channels for Farm Aid in Kansas lack adequate cultivation
Agilla- All the numbers came from Farm Aid directly. Their tax forms are with the article as well, if you want to check it out (on the upper left under the ad). The folks at Farm Aid were terrific and helpful in helping me unravel why Kansans haven't gotten much aid. We haven't asked. It's all there. Read the whole thing, please.
August 8, 2011 at 1:06 p.m. ( permalink | suggest removal )
Three Moves: Personal trainer offers tips to tone great glutes
Thanks for your eagle eyes, Tracey! The address is now fixed. It really is a great blog, check it out!
January 31, 2011 at 8:38 p.m. ( permalink | suggest removal )
'American Idol': The fix is on
Who do you think Siobahn's votes will go to, Jon? Crystal? Lee? Someone else?
May 5, 2010 at 9:54 a.m. ( permalink | suggest removal )
'American Idol' gets a little bit country
I'm kind of wondering if the judges were tough on Crystal because they are getting worried that people will think she has it in the bag and stop voting for her (Chris Daughtry, anyone?) and they don't have their "save" anymore in case that happens. I think Simon and the gang were hoping for reverse psychology a bit, like if they're tough on her, people might say "Oh, she was great, they're crazy, I'm voting for her!" ....
April 28, 2010 at 9:54 a.m. ( permalink | suggest removal )
Eating up the landscape: Permaculture proves a yard can wield more than grass
Smitty- When I talked with Lori last night, she said she was thinking of putting salvaged tree stumps in the area between the sidewalk and the curb. That way they could look neat (all different heights) and double as seating for visiting neighbors. As far as I'm aware by going through the design with them, there is no plan to have a fruit-bearing tree near the sidewalk area, so don't worry about having to walk through fallen fruit.
April 21, 2010 at 11:29 a.m. ( permalink | suggest removal )
Ranking the Top Ten 'Idol' finishers
Great breakdown, Jon!
March 31, 2010 at 11:47 a.m. ( permalink | suggest removal )
Snacking satisfaction: Dishes to indulge both the hungry hoops fans and the those with special diets
Ack! Sorry, about that Boston, we'll get that fixed. It's one pepper, cored and seeded.
March 17, 2010 at 10:09 a.m. ( permalink | suggest removal )
'Idol' contenders can't always get what they want
Please tell me that next week they'll have a smarter theme for singers ... sometimes this show doesn't exactly help itself!
March 17, 2010 at 8:52 a.m. ( permalink | suggest removal )
The Oscars: Welcome to the history books, Kathryn
Awesome job, Mel!
March 8, 2010 at 11:51 a.m. ( permalink | suggest removal )
Jew Ball! KU students put their own spin on Shabbat
Cheeseburger- You're right about the different spellings. The spelling in the story is correct. I'm unable to change the graphic — the designer who created it has to do that — but I'll see if it can be done on Monday. Good catch!
Sarah Henning
March 6, 2010 at 12:16 p.m. ( permalink | suggest removal )
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The trials and tribulations of a superhero kid and his evil twin.
weebl and bob
It's the everyday happenings of weebl and sometimes weebl's friend bob!
Jazza's epic sword and sorcery adventure!
i normally dont like super short movies...but this is the exception XD best. ending. ever.
Never though this movie is short. ^_^ Nice though.
jesus when did that red guy go on the movie reboot that please
great but could be longer great drawings


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H.R.1213 - To repeal mandatory funding provided to States in the Patient Protection and Affordable Care Act to establish American Health Benefit Exchanges.112th Congress (2011-2012)
|Rep. Upton, Fred [R-MI-6] (Introduced 03/29/2011)
|House - Energy and Commerce
|Committee Reports:
|H. Rept. 112-65
|Latest Action:
|05/05/2011 Read the second time. Placed on Senate Legislative Calendar under General Orders. Calendar No. 39.
|Major Recorded Votes:
|05/03/2011 : Passed House
This bill has the status Passed House
Here are the steps for Status of Legislation:
- Introduced
- Passed House
Subject — Policy Area:
- Health
- View subjects
Related Bills: H.R.1213 — 112th Congress (2011-2012)All Bill Information (Except Text)
No related bill information was received for H.R.1213.


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President Obama unveiled his plan to tackle climate change by reducing greenhouse gas emissions.» Read More
Eli Lilly has been on a roller-coaster ride in the wake of patent expirations, CEO John Lechleiter tells CNBC.
As the Super Bowl looms, real estate brokerage Redfin culled some of the prettiest property listings in Boston and Seattle.
Denver is becoming home to more regional corporate headquarters, creating demand for housing but the listings are light.
Federal Reserve Chair Janet Yellen offered an upbeat assessment on the U.S. economy in a meeting with Senate Democrats, according to media reports.
Valuations for energy companies may not have fallen far enough to spark dealmaking, two analysts tell CNBC.
The pace of business activity in the U.S. Midwest in January accelerated unexpectedly in January, a report showed on Friday.
The corporate "tax holiday" plan to pay for infrastructure projects is supported by Sens. Rand Paul and Barbara Boxer, the Journal says.
After years of giving investors the cold shoulder, Amazon.com is starting to warm up to Wall Street.
President Obama will dedicate $215 million in his budget request to a program that will target the underlying cause of disease.
Chevron reported fourth-quarter earnings and revenue that beat analysts' expectations on Friday.
The drugmaker has engaged in $100 billion of M&A in the last year, but CEO Brent Saunders told CNBC it is committed to innovation.
Here are the states that impose no personal income taxes.
Is Yahoo a takeover target. ESPN's Lee Corso always says: Not so fast, my friend.
Beware the dirty dozen tax scams of 2015.
Analysts are predicting Standard & Poor's 500 companies will see no earnings growth at all in the first quarter of 2015. Here's why.
Colleen McCullough is likely Australia's best-known writer, but one Australian daily thought her weight was more important than her work.
The US Senate has passed the final Keystone bill which now faces veto by President Obama.
McDonald's is struggling. Shake Shack's about to go public. What's really going on with the American staple: the burger?
Two new studies discuss turning stem cells into hair cells and transplanting them onto bald spots.
Get the best of CNBC in your inbox
Curtis "50 Cent" Jackson's latest bankruptcy filings reveal more financial details, including a net monthly income near $80K.
What's the state of the restaurant industry? It depends where you look.
The last remaining bottle of a vintage cognac made during the Battle of Trafalgar has gone on sale for £148,320.
The weakness in the oil market has caused this stock and many others to suffer.
Mad Money host Jim Cramer reminds investors there are always two sides to every coin. Why China, oil and Greece are all good for the bulls.
Mad Money host Jim Cramer revisits his infamous rant about the Fed from 2007. Has anything really changed?


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This cute little film reminding us of the power of choosing the right words is directed by Seth Gardner of redsnappa.com for Purplefeather.
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Minnesota's Five Division I College Hockey Teams To Make Announcement
March 21, 2013
The University of Minnesota, Minnesota State University - Mankato, St. Cloud State University, University of Minnesota Duluth and Bemidji State University will make a joint Minnesota college hockey announcement at a press conference on Saturday, March 23, at Xcel Energy Center. The press conference will begin at 5:15 p.m. or immediately following USA Hockey's 5 p.m. press conference.
The press conference will be held in the interview room at Xcel Energy Center.
WHAT: A joint Minnesota college hockey announcement
WHEN: March 23 at 5:15 p.m.
WHERE: Interview room in Xcel Energy Center
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Oak Lawn Housekeepers
Choose from 33 housekeepers in Oak Lawn, IL! Average rate: $12.25/hr. Join for free today and make sure to share this rate with friends!
Housekeeper — Oak Lawn, IL
I have worked for a cleaning business before. I have been helping clean my parents house as well for years, so I have a lot of experience. I will leave your house as clean as I can. I enjoy cleaning a...
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Quintin Berry Gets Tenth Man Award by Detroit Tigers
Two weeks ago, Detroit Tigers rookie Quintin Berry was announced as the recipient of the Detroit Sports Broadcasters' Associates Rookie of the Year Award. This week, it was announced that Berry will be given the Tenth Man Award by the Detroit Baseball Society.
Berry will receive the 2012 Tenth Man Award on January 26th. The award is meant to award a player who is not in the lineup every game, but still contributes. Last year, the award went to utility player Don Kelly.
In the 2012 season, Berry saw his first major league experience after spending seven years in the minors. The Tigers outfielder, who was drafted by the Philadelphia Phillies in the fifth round of the 2006 MLB Draft, was a key speedster for the team. Berry led the team with six triples and 21 stolen bases. Berry hit .258, with an impressive .330 on-base percentage. The Tigers player filled in nicely at all three outfield when injuries to Austin Jackson and Andy Dirks left the Tigers short. Berry also filled in at the leadoff hitter spot normally held by Jackson.
Berry became a fan favorite with his speed and dugout excitement. The Tigers outfielder hit safely in his first six games in the majors. After Jackson returned from injury, Berry was seldom used and saw his statistics decline.
The tenth man award could be a curse for Tigers players. Kelly was not re-signed by the Tigers during the off-season and Berry now finds himself in a crowded outfield position battle with the team. Berry contributed when needed for the Tigers and will hopefully be rewarded with some starts in the 2013 season.
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