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A device and method for a self contained solid phase immunodiffusion assay. The device is comprised of a sample collector, and a pre-fabricated laminate which can be used in many different forms. For example, the same collector and laminate can be used with a tube having compartmentalized reagents. The seals can be broken through pressure on the sample collector. The sample collector is pushed through the seals, mixed with reagent, and then pushed into a ligand receptor reaction area which is part of the laminate. The tip of the sample collector contacts diffusable or porous membranes or filters and transfers the reactants to a capture membrane wherein a ligand receptor reaction can be examined by the naked eye or otherwise.
1. A method of performing a ligand receptor assay in an assay test device wherein a ligand receptor complex is captured on at least one porous capture membrane in the test device, comprising the steps of providing sample collector means, collecting a specimen with said sample collector means and contacting the specimen with one or more reactants as required to perform said assay; providing at least one porous membrane located in the test device as required to perform said assay to capture any ligand receptor complex formed as a result of the performance of this and the preceding steps; causing liquid diffusion in the assay test device from the specimen and the reactants on said sample collector means to all of said at least one porous membrane simultaneously to capture said ligand receptor complex while the membrane is a part of the test device; and examining all of said at least one porous membrane after performance of all of the preceding steps while said at least one porous membrane is a part of the test device; whereby any change caused by a labelled ligand or a labelled receptor being captured or not being captured on said at least one membrane in accordance with the protocol of said assay can be readily observed.
2. The method of claim 1, and the additional steps of providing two of said porous membranes, and causing the other of said two porous membranes to be a control membrane.
3. The method of claim 1, and the additional step of filtering said specimens and reactants during said liquid diffusion step using filter means having a pore size selected to be large enough to permit said liquid diffusion step and small enough to prevent debris larger than said selected pore size which may be on said sample collector means from contacting any of said at least one porous membrane.
4. The method of claim 1, and performing said liquid diffusion step by including the sub-step of creating physical pressure at least indirectly between said sample collector means and all of said at least one porous membrane simultaneously.
5. The method of claim 1, and performing the method using a pre-fabricated assay device, and constructing said assay device comprising the following steps and providing sequential layers as follows:
(a) said at least one porous membrane;
b) impervious shield means;
c) forming at least one opening in said impervious shield means equal in number to the number of said at least one porous membrane and sizing and clustering said at least one opening so that said liquid diffusion can be performed through all of said at least one opening simultaneously;
d) filter means; and
e) selecting a pore size for said filter means large enough to permit said liquid diffusion and small enough to prevent debris larger than said selected pore size which may be on the sample collector means from contacting any of said at least one porous membrane.
6. The method of claim 5, and the additional steps performed during said construction of said pre-fabricated assay device of providing an absorbent means layer, and locating said absorbent means layer next to said at least one porous membrane on the side thereof opposite said impervious shield means.
7. A pre-fabricated ligand receptor assay device comprising sequential layers of:
a) at least one porous membrane adapted to capture any ligand receptor complex formed during the use of said assay device,
i) impervious shield means formed with at least one opening equal in number to the number of said at least one porous membrane,
ii) means for sizing and clustering said at least one opening so that liquid diffusion between said at least one porous membrane and a reactant brought into contact with said assay device can occur through all of said at least one opening simultaneously,
c) filter means having a pore size large enough to permit said liquid diffusion and small enough to prevent debris larger than said selected pore size from contacting any of said at least one porous membrane,
a sample collection means comprising an elongated shaft and an absorbent tip, and a sandwich device, said sandwich device comprising a pair of plate means, means to hinge said plate means together along one respective side of said pair of plate means, positioning said pre-fabricated assay device between said plate means, and means to apply pressure to said tip when said tip is inserted between said pair of plate means and in contact with said pre-fabricated assay device.
8. The device of claim 7, and means to apply pressure to said tip when said tip is in contact with said assay device, and said means to apply pressure comprising contoured protrusion means adapted to bear on said tip.
9. A kit containing the device of claim 7, said kit including a container containing liquid labelled receptor or labelled ligand reagent for cooperation with said sample collection means and selected in accordance with the protocol of said assay.
10. The device of claim 9, wherein said labelled reagent comprises a chromophore.
11. The device of claim 10, wherein said chromophore comprises a dye.
12. The device of claim 10, wherein said chromophore comprises a dyed particle.
13. The device of claim 10, wherein said chromophore comprises a pigment.
14. The device of claim 10, wherein said chromophore comprises a metal sol particle.
15. The device of claim 10, wherein said chromophore comprises a dye encapsulated liposome.
16. The device of claim 7, and said pre-fabricated assay device including an absorbent layer in contact with said at least one porous membrane on the side thereof opposite said impervious shield.
17. The device in claim 7, said means to apply pressure comprising clamping means.
18. The device of claim 7, and a second porous membrane, said second porous membrane comprising a control membrane.
19. The device of claim 7, and said assay device including an absorbent layer in contact with said at least one porous membrane on the side thereof opposite said impervious shield.
20. The device of claim 19, wherein at least said impervious shield, said at least one membrane and said absorbent layer are prefabricated in the form of a laminate.
21. The device of claim 7, and said means to apply pressure comprising contoured protrusion means adapted to bear on said tip.
This application is a continuation of U.S. patent application Ser. No. 07/262,503 filed as PCT/US87/03169, Dec. 1, 1987 (now abandoned); and that application Ser. No. 07/262,503 is a continuation-in-part of U.S. patent application Ser. No. 06/938,003 filed Dec. 3, 1986 now U.S. Pat. No. 4,770,853; all of the entire disclosures of which are hereby incorporated by reference as if here set forth in full.
In order to determine the condition of a patient, and to minimize the diseased state, the need for a rapid diagnosis and appropriate treatment by health care professionals is apparent. Diagnosis of many conditions can be facilitated through the determination or quantitation of antibodies, antigens, nucleotide fragments, and other analytes from a biological specimen, which are indicative of a particular disease state or condition. A rapid, sensitive, specific, and simplistic assay is extremely useful for emergency situations, field testing, physicians' offices and in home diagnostics. As diagnostic tests become more simple and easier to perform, they are being performed away from the professional clinical laboratory setting to physicians' offices and even to the home, where untrained or poorly trained individuals perform the tests, usually following product insert instructions alone. These assays are useful, provided they are performed properly and are safe to handle for the user. Assays that require multiple steps, have multiple reagents, and have limited storage conditions are prone to misuse, especially if they are performed by individuals without adequate training or skills.
Many types of ligand receptor assays have been developed and commercialized. These assays are less expensive if capital equipment can be eliminated, such as scintillation counters, fluorometers, and colorimeters in the case of radioimmunoassay, fluorescent immunoassay, and enzyme immunoassay respectively. Non instrumental assays, such as latex agglutination, enzyme immunoassays on strips, tubes, membrane or filters have increased the usefulness and ease of performance of immunodiagnostic testing, but are still cumbersome requiring washing steps, multiple reagent additions and usually refrigerated storage conditions.
In some assays amplification or growth of viruses and bacteria are desirable before testing to increase the sensitivity of detection. In other assays adsorption steps to remove interferring substances or inhibitors of the ligand receptor assay, or long incubation of reagents are necessary to perform an assay. Each step for an assay increases the difficulty of testing for the minimally trained individual and any device that would reduce user error would improve diagnostic testing.
Horrisberger et al (J. Histo Cytochem volume 25: 295-305, 1977) described the use of colloidal gold particles in an immunoassay. Leuvering in U.S. Pat. No. 4,313,734 also describes such an immunoassay. Cerny in PCT/US patent application number 85/02534 describes a solid phase diffusion assay using gold sol particles as an immunolabel which can be visualized by the naked eye on a capture membrane, and requires no washing step. Bernstein et al (86th annual American Society for Microbiology Meeting, 1986) presented and described a rapid immunodiffusion enzyme labeled antibody assay for Group A streptococci on a membrane in which there is no washing step. Gould and Zuk in U.S. Pat. No. 4,552,839 describe the use of colored or dyed beads in a solid phase immunoassay. Through the introduction of colored immunolabelled binding reagents (i.e. gold sol particles, dyed particles, dye encapsulated liposomes, etc.) and the removal of washing steps it becomes possible to perform receptor ligand assays in a closed system with the sequential additions of all reagents within that system.
A number of antigens of interest in the diagnosis of infectious disease are collected with a sterile swab on a shaft to remove the organisms from the suspected infected area or test site (wounds, lesions, blood, tissues, pus, fluids, etc.). The swab is generally used to transfer organisms to a suitable media for culturing which may take as long as 48 hours for growth of bacteria, and 2 weeks for viruses. If the organisms are viable and do grow, then their identification could be made by biochemical, morphological or immunological methods. This time consuming method is slowly becoming replaced by more rapid immunological testing methods or DNA probe methodologies.
In many immunoassays that utilize a swab for collection of antigens or cells, the swab is placed in a solution to release the antigenic materials or cells after collection. It may be necessary to use enzymes, acids, detergents, etc. to solubilize or breakdown the antigens to expose antigenic determinants. The extracted material can then be used in an immunoassay by removing the fluid from the swab and mixing it with other reagents or adding the other reagents directly to the swab extract. In the case where membrane or filters are used to capture the immunoreactants, it is necessary to bring the fluid containing the immunoreactants in contact with the filter or membrane.
In addition, where extraneous cells or debris may interfere with an assay, it may be necessary to have a prefilter (larger pore size filter or membrane) present between the swab and the capture membrane or capture filter to retain these unwanted components.
In some assays, where antigen expression may be low, amplification can be achieved if the organisms are first cultured and then tested. If the culturing and the testing could be performed in a single device, then testing would be simplified. In some assays where there are inhibitors, cross reactive products, or clotting factors, red blood cells, etc., it may be necessary to add adsorbant materials (i.e. beads, kaolin, antibody coated particles, antigen coated particles, or lectin coated particles), anticoagulants, or buffers etc. before the ligand receptor assay can be performed.
It is an object of the present invention, in one preferred form, to provide a novel test device that utilizes a swab or swab-like material (a shaft with a porous or fibrous absorbant material at one end) to collect a sample and to be able to react the sample on the swab with all the necessary reagents which are included within the device, and then to use the swab to transfer the reactants sequentially to other reactants if necessary, and finally to a reaction zone where the specific labelled reactant can be captured and visualized.
It is another object of the present invention, in a second preferred form, to provide a prefabricated assay device in the form of a laminate. This laminate, in cooperation with a swab or other sample collector device, provides a means for performing many types of ligand receptor assays. The laminate can be used in many different assay formats.
It is a further object of the present invention to provide a test device useful in performing ligand receptor assays to detect antigens, haptens, antibodies, DNA or RNA fragments, and the like, wherein the user is not required to dispense any of the reagents.
It is a particular object of the present invention to provide a test device that can be stored at nonrefrigerated temperatures, and can be utilized to perform an assay on a biological specimen or fluid without any additional reagents having to be provided to the test device.
In addition it is a further object of the present invention to provide a test device which can utilize lyophilized reagents that can be reconstituted in situ within the device.
The present invention maximizes the safety and ease of performance of ligand receptor assays through the use of apparatus designed to enable a biological specimen to be obtained by a collection device comprising a shaft and an attached tip of adsorbent or absorbent porous or fibrous material (i.e. rayon, dacron, cotton swab). In the first form of the invention, the tip is inserted into a cylindrical tube. The cylindrical tube contains a sealed vessel, or chamber, or plurality of sealed vessels or chambers in sequential order. The seal will break away or collapse when pressure of the collection device (swab) is exerted on the seal by physically pushing the collection device into and through each vessel. These sealed vessels may contain media, extraction reagents, diluents, labelled antibodies, labelled antigens, labelled lectins, anticoagulants, adsorbants, inactivators, etc. which mix with the biological specimen collected on the collection device. The reagents in these vessels may be lyophilized, enabling long term storage at non refrigerated temperature. The vessels are fixed in position in the cylindrical tube to enable the seals to be broken when physical pressure is exerted on the shaft of the collection device. The collection device holder has appropriate stop points to allow for the collection device tip to enter the appropriate vessel and mix with its contents. A key feature of the vessels are that the tip and shaft of the collection device can pass through each of the vessels into a lower portion of the cylindrical tube and attached lower portion comprising a ligand receptor reaction area. This ligand receptor area is part of a pre-fabricated laminated assay device.
The ligand receptor area is comprised of a capture membrane or a filter that will allow unbound reactants to pass through by diffusion and retain the appropriate labelled members of the binding pair. The capturing membrane or filter may be coated with a member of the binding pair to capture the reactants. If capture particles are used, then the capture filter is utilized to retain the particles and allow unbound free labelled antigen or antibody to diffuse through. A prefilter may be used between the collector tip and the capture or filter to remove any nonspecific binding due to debris. An additional absorbant material can be placed behind the capture membrane to increase the uptake of fluid. In either case a specific volume of reactants can be absorbed by controlling the size of the filter and absorbant materials. The configuration of the lower portion allows the collection device to come into physical contact with the prefilter, capture membrane or capture filter. The pre-fabricated assay device is comprised of sequential layers of at least one porous membrane, an impervious shield having at least one opening, and a filter means.
FIG. 1 is a cross-sectional view of a first form of the invention showing the collection device holder, the collection device, the tube, the sealed reagent compartments and the lower ligand receptor transfer area;
FIG. 2 is a perspective view of the basic structure of the collection device holder and collection device of FIG. 1 including the grooves for guiding the movement of the collection device through the apparatus;
FIG. 3 is a perspective view of the basic structure of the tube of FIG. 1, its compartmentalized reagents, and the nodule which fits into the groove of the collection device holder;
FIG. 4 is a perspective view of the sealed compartments (i.e. vessels) of the apparatus of FIG. 1;
FIG. 5 is a cross-sectional side view of the lower portion of the apparatus of FIG. 1 showing the final position of the collection device tip at the window of the ligand receptor area;
FIG. 6 is an exploded perspective view of the ligand receptor test area as used in the apparatus in FIG. 1;
FIG. 7 is an exploded perspective view of the pre-fabricated assay device according to a second form of the invention;
FIG. 8 is a elevational view of the sample collection device in contact with the pre-fabricated assay device of the second form of the invention;
FIG. 9 is a perspective view of a prefabricated assay device and sample collection device being sandwiched between an upper hydrophobic pressure plate 29 and a lower flat hydrophobic plate 30 according to a third form of the invention; and
FIG. 10 is perspective view of a prefabricated assay device showing a modification of the third form of the invention having a sample collection device positioned on a lower flat hydrophobic plate 30 which is hinged to a hydrophobic upper pressure plate 29.
In the following description, the various forms of the apparatus and the method of the invention will be described in exemplary terms only, for an antigen determining an immunoassay test. This discussion, however, is simply to illustrate the structure and use of the apparatus and the technique and steps of the method. The various forms of the apparatus clearly can be used for any ligand receptor assay in which washing steps have been eliminated and transfer of the reactants to or through a porous membrane or filter is used. The best modes, as described hereinafter, are accordingly, to be considered exemplary and not limiting as to the scope and concept of the invention.
Referring first to FIG. 1 for a general depiction of the apparatus, the inventive apparatus comprises a collection device holder 14 which is comprised of a restrictive portion 1 that hold the shaft of the collection device 2 in place, a cylindrical tube 13 which is comprised of one or more sealed reagent compartments 15 and 20, and a lower ligand receptor reaction area 10. Area 10 is drawn enlarged, but can be smaller, see FIG. 5 for example.
Referring to FIG. 2, the collection device holder 14 has a nodule 16 which positions onto the cylindrical tube and prevents the apparatus from being accidentally opened. Nodule 16 can be heat sealed or made to cooperate with a groove formed in tube 13, or the cooperating nodule 17 shown in FIG. 3 or other means can be provided to accomplish the sealing function. When a sample is to be taken, the collection device holder is removed and separated from the cylindrical tube by twisting and pulling up on the collection device holder. This frees up the collection device holder which is then used to collect the test sample (i.e. throat swab, pus, blood, urethral swab, etc.) by allowing the collection device tip 5 to come into contact with the suspect tissue, fluid, wound, etc.
Referring to FIG. 2 and FIG. 3, after obtaining a test sample, the collection device holder is replaced onto the cylindrical tube 13 and turned until the nodule 4 (FIG. 3) on the cylindrical tube is in alignment with the groove 18. The collection device holder is then manually forced downward until the nodule 4 stops at the horizontal groove 19. When the nodule 4 is in contact with horizontal groove 19, then simultaneously the tip 5 will have broken through the first seal (FIG. 4), mixing with the contents of the first chamber 15, then breaking through seal 7 and emptying its contents into chamber 20. The number of independent chambers is related to the number of required reagent additions and incubation steps. One chamber or a plurality of chambers could be used and the mixing of reagents controlled using the principles of nodule 4 and grooves 18, 19 as previously described. In the preferred embodiment, the collection device holder is turned to the right and then back and forth using groove 19 to mix the contents of vessel 20 through the simultaneous turning of the collection device tip.
Referring to FIGS. 2 and 3, after an appropriate incubation time, the collection device holder 14 is turned to the right and thus aligning nodule 4 (FIG. 3) with groove 3 (FIG. 2) and then manually forced downward until the movement of nodule 4 is stopped by the groove end 21 (FIG. 2).
Referring to FIGS. 5 and 6 the lower portion 10 may be physically one piece with the cylindrical tube 13 or may be an attached separate piece. When the nodule 4 is in contact with the groove end 21, then the collection device tip 5 is in contact with the prefilter membrane 25 through the window 11. The reactants flow through the prefilter membrane through holes 24 of impervious shield 23 which holds the prefilter membrane 25 against hole or window 11. The shape of the lower portion 10 is configured to enhance contact of the collection device tip 5 with the prefilter or reaction membranes. If preferred, the prefilter could be placed on the inside wall of the window 11. In any case, the reactants flow through holes 21 and 22 of shield 20 which holds membranes 18 and 19 respectively in place. The holes 21 and 22 restrict the flow of the reactants through a capture membrane 19 and a control membrane 18 and enhance the signal of the reaction by concentrating the labelled ligand or receptor binding pairs into a small area. Absorbant 17 absorbs excess fluid diffusing through the membranes. When an appropriate volume of fluid has diffused through the membranes, usually by saturation of the absorbant, the capture and control membrane are examined within the holes 21 and 22 respectively by lifting the tab 28 of the adhesive tape 12.
In the preferred form of the invention, the assay results are examined by the naked eye since color changes are the result. However the invention is not limited in this manner and the "examination" of test results could be done with instruments or otherwise dependent upon the protocol of the particular assay.
Adhesive tape 12 holds the absorbant 17 in place and applies the necessary pressure to insure diffusion of fluid through the various layers of the ligand receptor test device 33A. The color intensity of the capture membrane 18 is compared to the color intensity of the control membrane 19. A positive result is determined by viewing a more intense color in the capture membrane than in the control membrane. A negative result is determined by no significant color or the same weak color in the capture and control membranes. In competitive inhibition assays the positive and negative results are reversed. In the performance of drug analyte assays, the size of the ring of color in a single larger capture membrane is related to the concentration of drug in the test sample. The design of the ligand receptor area, the coating of reagents on the membranes, and the addition or deletion of capture or control membranes are dependent on the particular type of assay being performed. The capture membrane can be coated with antigen or antibody, or other complementary ligands or receptors and can be used to determine the presence of different antigens or antibodies. The number of chambers used in the apparatus is dependent upon the type of assay and can contain diluents, media for growth amplification of microorganisms, lyophilized labelled ligands or receptors, etc. The seal 7 (FIG. 4) may be attached to two chambers simultaneously or may be independent. Therefore the chambers could be attached to each other or be independent.
Referring to FIG. 6, the prefilter 25 is fixed around the periphery of hole 11 in tube 10 by taping, gluing, heat sealing, chemically sealing, sonic welding, or the like. Impervious shield 23 is a single side adhesive coated laminate having adhesive on the side facing the prefilter 25. The impervious shield laminate 23 with hole 24 fixes and exposes the prefilter to the tube 10 and hole 11. The impervious shield 20 can also be a single side adhesive coated laminate which fixes membranes 18 and 19 onto absorbent 17. Absorbent 17 is fixed on the side opposite to the positioned membranes with adhesive tape 12. The adhesive tape 12 fixes the assay device 33A to tube 10. Alternative means of fixing can include clamps, glues, or the like. In order to examine a test result on the membranes 18 and 19 it is necessary to separate the impervious shields 23 and 20 from each other to expose the membranes. By pulling back adhesive tape 12, a portion of the assay device 33A consisting of shield 20, membranes 18 and 19, and absorbent 17 separates from filter 25 and shield 23, thereby exposing membranes 18 and 19 for examination.
Referring to FIG. 7, the pre-fabricated assay device 33 is a laminate comprised of a prefilter membrane 25 held in place by a hydrophobic impervious shield 23 having a hole 24 through which reactants from absorbent tip 5 can flow into the prefilter. When absorbent tip 5 is pressed into contact with prefilter 25, the flexible prefilter is forced into contact through holes 21 and 22 selectively clustered and sized in impervious shield 20 with control and capture membranes 18 and 19. The fluid reactants from tip 5 flow from prefilter 25 to both membranes 18 and 19 simultaneously. Absorbent 17 is in contact with membranes 18 and 19 and the fluid reactants diffuse through membranes 18 and 19 to absorbent 17. At the conclusion of a ligand receptor assay the impervious shield 23 with attached prefilter 25 is removed and membranes 18 and 19 are examined by the naked eye, or otherwise, for any color change, or the like.
FIG. 8 is a perspective view of a collection device tip 5 being manually brought into contact with an assembled pre-fabricated assay device 33. The fluid of a labelled ligand or receptor reagent in combination with sample is transferred from tip 5 through prefilter membrane 25 and through control and capture membranes 18 and 19 to absorbent 17. This shows the general utility of the invention devices 33 and 33A to be used in many different environments.
FIG. 9 is a perspective view of a sandwich device 36 for holding collection device tip 5 under pressure against a pre-fabricated assay device 33. Hydrophobic upper pressure plate 29 is movable and hinged to hydrophobic lower flat plate 30. After reacting sample collection device tip 5 with labelled ligand or labelled receptor by manipulating shaft 2, the sample collection device tip 5 is positioned horizontally in contact with prefilter membrane 25 and pressure is applied to the device forcing the absorbent tip 5 to push the prefilter 25 into contact with porous membranes 18 and 19 and allowing the reactants to flow through and into the pre-fabricated assay device 33. Pressure can be applied to pressure plate 29 by means of a weight, clamp, manually squeezing, or the like. After an appropriate period of time to allow the fluid reactants to penetrate the assay device 33, pressure is removed from the hinged sandwich device and the impervious shield 23 is lifted to allow the control and capture membranes of the pre-fabricated assay device to be examined.
FIG. 10 is a perspective view of a hinged sandwich device 37 for applying pressure to sample collection device tip 5 and the pre-fabricated assay device 33. Upper hydrophobic pressure plate 29 is contoured as at 31 to the sample collection device 2 and 5 to maximize the amount of fluid that can flow into the pre-fabricated assay device 33 which is positioned on the lower hydrophobic plate 30. Upper plate 29 is formed with a groove 31 to accept shaft 2 of the collection device, and with a composite male and female protrusion 32 contoured to accommodate shaft 2 while permitting the application of pressure to squeeze tip 5. After the sample collection device has reacted with labelled ligand or labelled receptor the sample collection device is manually placed within the hinged sandwich device 37 with sample collection device tip 5 in contact with prefilter membrane 25 and pressure is applied to and maintained by the hinged sandwich device 37. The hinged sandwich device 37 consists of a hinge 32a and a clamping mechanism such as protrusions 34 from upper pressure plate 29 and a complementary fitted piece such as the orifices 35 which will produce a friction fit and maintain the closed clamped position of the hinged sandwich device 37. After an appropriate time period the hinged sandwich device is opened and the prefilter membrane 25 is removed to expose the control and capture membranes of the pre-fabricated assay device 33.
In FIGS. 9 and 10, the assay device 33 can be layed loosely, or affixed as by glueing or the like to one of the plates 29 or 30. Also a form fitted depression to hold the device 33 could be formed in a plate 29 or 30.
In the following section certain terms used in the specification and claims herein shall be understood to have the following meanings:
Ligand: Any organic compound for which a receptor naturally exists or can be prepared including haptens, antigens, sugars, vitamins, peptides and the like.
Receptor: Any compound capable of recognizing a specific molecular configuration of a ligand including antibodies, lectins, enzymes, nucleic acids, Fab fragments, avidin and the like.
Ligand receptor reaction: Any binding between a receptor and its complementary ligand.
Porous membrane: any porous solid matrix including porous spreding layers, bibulous papers, filters, and the like.
Coated porous membrane: A porous membrane that has a ligand or receptor covalently or noncovalently attached to its surface.
Impervious shield: any hydrophobic material that will not allow diffusion of fluid to occur through its surface. This material can be plastic, a plastic adhesive laminate, and the like.
Chamber: a partitioned space, vessel, reservoir, compartment or the like.
The following example is illustrative:
Group C phage associated lysin enzyme which is effective in fragmenting and solubilizing the Group A streptococcal polysaccharide was diluted in a buffer of .05M citrate phosphate pH 6.1 containing 0.005 EDTA, 0.005 DTT, 0.1% rabbit IgG, 0.05% sodium azide and mixed with rabbit anti streptococcal Group A coated gold sol particles (OD518 1.5) diluted in a buffer of 0.02 Tris pH 8.2 containing 1.0% BSA, 0.2% sodium heparin, 0.5% n-acetylglucosamine and 0.02% sodium azide in a ratio of 3 parts lysin reagent to 1 part antibody gold sol reagent. The combined reagent was sterile filtered through a 0.2 micron cellulose acetate filter and 200 microliters were aliquoted into acrylic walled reaction cup vessels, having an aluminum foil sealed bottom. The aliquots were frozen and lyophilized. The reaction cup vessels were sealed with aluminum foil and contact cement under nitrogen. Another reaction vessel was cemented to the aluminum foil lid of the first vessel. Two hundred microliters of distilled water was added to the second vessel and then cemented and sealed with aluminum foil. The vessels were placed and positioned into the cylindrical tube. The ligand receptor area was prepared by coating nitrocellulose membranes with rabbit anti Group A streptococcal antibody for the capture membranes, and normal rabbit immunoglobulin for the control membrane. The membranes were dried and fixed to a diacetate laminate which had 1.5 mm diameter holes for each membrane. A 1.2 micron cellulose acetate prefilter was used to cover the hole (window) in the lower portion of the tube. A dacron tipped swab was seeded with varying concentrations of group A streptococci. The swab was placed into the cylindrical tube and forced downward to break the first two seals on the reaction vessels. The swab was incubated for 4 minutes at room temperature allowing the lysin enzyme to solubilize the Group A streptococcal polysaccharide and the reaction of the gold labelled anti Group A antibody to form complexes with the released polysaccharide. After the four minutes, the swab was forced downward through the third seal into the lower portion, coming in contact with the ligand receptor area 33. The fluid diffused through the prefilter into the capture and control membranes. After 30 seconds the tab 28 on the ligand receptor area 33 was pulled away from the lower portion and examined by eye. A distinct color reaction with 2�103 organisms of Group A streptococci could be distinguished in the capture membrane compared to the colorless control membrane.
The foregoing disclosure and the showing made in the drawings are merely illustrative of the principles of this invention and are not to be interpreted in a limiting sense. It is to be understood that, through the example and embodiments described herein, various modifications in light thereof will be suggested to persons skilled in the art. Thus, it is to be understood that the protection granted is to be limited only within the spirit of the invention and the scope of the following claims.
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Originally Posted by tkamiya
For whatever reason, KEH is constantly running low (or non-existent) on RB backs for quite a while now. As of right now, there are none left and when one shows up, it goes fairly quickly. Be quick about it if you want one.
... and oh yeah, you'll be competing with me on quickness!
I noticed that...
BUT, I have an inside track to sales there ... LOL
Go to the light......

subset: commoncrawl


text:
Directed by: Christopher Nolan
Written by: Christopher Nolan and Jonathan Nolan
Main Cast: Matthew McConaughey, Anne Hathaway, Jessica Chastain, Michael Caine, John Lithgow, Mackenzie Foy, Bill Irwin, Wes Bentley, Casey Affleck, Matt Damon, David Gyasi, Topher Grace, Ellen Burstyn, and David Oyelowo, among others…
Past Oscar relations: Burstyn, Caine, Damon, Hathaway, and McConaughey are previous Oscar winners, while Affleck, Chastain, and both Nolans are Academy Award nominees in prior years
Today we have another brand spanking new article in this particular series of mine concerning certain high profile 2014 releases hoping to compete for some sort of actual Oscar attention as a contender at the upcoming 2015 ceremony. Next up for here for us is one of the biggest contenders of the year in Interstellar. It's an epic that's hoping to become the blockbuster in the Best Picture race. Does it have a strong chance? Well, let me get into that a little bit now…
This movie is a science fiction adventure/drama set in the near-ish future. Earth is dying and a last ditch mission to save it is set up by what remains of NASA. A former engineer and pilot turned farmer is chosen to lead the way and must leave his children behind in order to save mankind. I could say more, but the less you know going in, the more wonder that's going to be in store for you all. Interstellar stars Matthew McConaughey, Anne Hathaway, Jessica Chastain, Michael Caine, John Lithgow, Mackenzie Foy, Bill Irwin, Wes Bentley, Casey Affleck, Matt Damon, David Gyasi, Topher Grace, Ellen Burstyn, Timothée Chalamet, David Oyelowo, and Collette Wolfe. Christopher Nolan directs and co-writes with his brother Jonathan Nolan. They all combine to literally make magic happen. Now, will Oscar recognize these achievements?
What this film has going in its favor is the incredible ambition on display. Not only do I feel that this is Nolan's best work behind the camera to date, it's also his most emotional by far. There's a ton of heart here, which voters will probably respond to. Not only is his direction flawless, the writing is complex and super compelling. Throw in the top notch performances, especially by McConaughey (as well as by Chastain and Damon), and that makes for across the board potential, including the technical achievements of cinematographer Hoyte van Hoytema, composer Hans Zimmer, and editor Lee Smith, just to name a few. If the Academy goes for this flick […]
Tag Archives: matt damon
"Interstellar": What's Up with the 2015 Awards Race
Directed by: Christopher Nolan
Could 'Interstellar' be an Oscar winner?
Last week, I was one of the lucky first members of the press to lay eyes on Christopher Nolan's new opus Interstellar. Now that the embargo has lifted, I can say without hesitation that it's his best work to date, an absolutely enthralling and deeply moving science fiction drama that makes both your heart and your mind soar. More important than my thoughts though (which I'll get into more below), is how its debut could impact the Oscar race. I know that only a few of my fellow pundits love it as much as I do and some aren't even fans of it at all, but this is a legitimate contender folks. The only question is to what extent…
For those somehow in the dark about this, Interstellar is a sci-fi movie set in the future from Christopher Nolan. It details a dying Earth and the last ditch attempt to save it by going in search of a new planet to colonize. Matthew McConaughey stars as the pilot turned farmer/single dad turned hero, with an incredible supporting cast that includes Anne Hathaway, Jessica Chastain, Michael Caine, John Lithgow, Mackenzie Foy, Bill Irwin, Wes Bentley, Casey Affleck, Matt Damon, David Gyasi, Topher Grace, Ellen Burstyn, and David Oyelowo. Nolan directs and co-writes with his brother Jonathan Nolan.
Even with high expectations, I was floored by this film. It's visually stunning, powerful, and surprisingly emotional. Nolan has never been one to move me to tears, but on more than one occasion I got choked up. Particularly in terms of how he deals with relationships between fathers and daughters (McConaughey and Chastain/Foye, as well as Caine and Hathaway), it's actually quite beautiful and sad. That's really something special and unique, compounded by how intelligent the science fiction is here. The details about black holes and the Theory of Relativity is handled with the sort of faith that assumed the audience will come on this journey without turning off their brains. If they/you do, you'll be in for something amazing.
So how can it impact the Oscar race? Well, it'll depend on how the precursors and guilds take to it, but there's a potential narrative where it could become one of the top contenders for Best Picture as well as Best Director. If all goes perfectly for Interstellar from this point on, it could be in line for up to a dozen Academy Award nominations. Pie in […]
Mark Ruffalo: 2015 Best Supporting Actor contenders
As you fine folks all already know by now, it's one thing to just read early Academy Award predictions at this point in the year in order to see what folks like myself think will happen in six months, but it's an entirely separate thing to actually know something about who and what will be in contention. To help out in that particular regard, I'm continuing to run down some of the major contenders in each Oscar category in order to prep you all for the season to come. Basically, the format will have me saying a few words about what or who I feel are the top tier contenders right now in said categories, along with a longer list afterwards of many of the other hopefuls that the Academy might potentially take a shine to. Consider this a sort of before the awards season cheat sheet to have in your back pocket.
Today I'm continuing with another of the bigger acting categories…yes, it's Best Supporting Actor.
Here are the ten particular thespians that I have in play for Best Supporting Actor, with the top five cracking the unofficial lineup at this point:
1. Mark Ruffalo (Foxcatcher) – Especially considering that I've recently been informed that Channing Tatum (who I'd previously been predicting for one of the other Supporting slots) is going Lead here, this category seems like Ruffalo's to lose right now. He's someone who fits as a winner, apparently is excellent in Foxcatcher, and is just a warm person to be around. That's a big help, as he'd be great on the campaign trail. At this juncture, Ruffalo makes a lot of sense as the number one here, so that's where I have him currently.
2. J.K. Simmons (Whiplash) – If there's an under the radar contender of sorts who can really upend the race, it's Simmons, who blew folks away at Sundance in Whiplash. A few people out there even have him winning (including myself at one earlier point), though right now he has to be behind Ruffalo. Simmons is overdue his first citation, so I have a feeling that his run of bad luck is about to come to an end. He deserved to be in bigger play for Juno, but now could very well be his time.
3. Christoph Waltz (Big Eyes) *could go Lead – It seems a little unlikely that Waltz could wind up winning his third Best […]
The 2015 Awards Race: Best Picture contenders – Unbroken, Foxcatcher
It's one thing to read early Academy Award predictions to see what folks like myself think will happen at the end of the year, but it's another thing entirely to actually know which films will be in contention. To that end, once or twice a week for the next month or so I'll be running down some of the major contenders in each Oscar category. Basically, the format will have me saying a few words about what/who I feel are the top tier contenders right now in said categories, along with a longer list afterwards of many of the other hopefuls. Consider this a sort of pre awards season cheat sheet.
Today I'm beginning with the big one…Best Picture.
Here are the ten films that I have right now cracking the Best Picture lineup:
1. Unbroken – To me, Angelina Jolie's sophomore directorial outing is the one to beat out right now. It's a World War II survival tale starring up and comer Jack O'Connell, written by a group that includes the Coen Brothers, and is shot by legendary cinematographer Roger Deakins. There's such a tremendous pedigree on display, this would have to be an absolute disaster not to crack the lineup. As it stands now, it's the one I have winning it all.
2. Foxcatcher – Bennett Miller's passion project got a nice boost this past week at the Cannes Film Festival. Raves were given not just for Miller, but for star Steve Carell and co-stars Mark Ruffalo/Channing Tatum as well, so there's across the board potential here. Right now, it's the only film that I'm basically 100% sure will be in the Best Picture lineup, barring something unexpected.
3. Men, Women, & Children – I'm perhaps higher on Jason Reitman's latest than most, but I'm also a big fan of the book by Chad Kultgen and see real potential here. Along with giving Adam Sandler a rare chance to be serious, the talented ensemble cast includes Rosemarie DeWitt, Jennifer Garner, Judy Greer, Dean Norris, Emma Thompson, Kaitlyn Dever, Ansel Elgort, Dennis Haysbert, and J.K. Simmons, so Reitman has tons of toys to play with here. If Oscar can handle the sexual frankness of the story, Ivan's son could again be a nominee and see his film nominated for Best Picture.
4. Big Eyes – Of late, Tim Burton movies have been the exact opposite of Oscar bait, but this sees him returning to something […]
Critics' Choice: Joey Berlin from BFCA
Joey Berlin from the Broadcast Film Critics Association – Critics' Choice Awards
By ROBERT W. WELKOS
In 1995, Joey Berlin and Rod Lurie, two entertainment reporters and film critics who had written for rival publications, joined forces to create the Broadcast Film Critics Assn.
While Lurie would go on to carve out a successful Hollywood career as a screenwriter and director with such films as "The Contender" and "The Last Castle," Berlin hasn't faired too badly, either, as the president of the BFCA.
Starting with 44 members the first year, the BFCA's voting membership today hovers between 285 and 290 and the organization bills itself as the largest film critics group in the U.S. and Canada.
At the same time, the BFCA created the Critics' Choice Awards, a glitzy tux-and-gown gala now televised on the CW Network that features red carpet celebrity arrivals, drawing worldwide media attention and plenty of Oscar buzz since the show coincides with the run-up to the Academy Awards.
But as the show has catapulted the broadcast critics into the Oscar conversation each movies awards season, tax records show that the nonprofit group Berlin leads has paid his privately-owned company, Berlin Entertainment, Inc., hundreds of thousands of dollars each year for production services.
Read prior Broadcast Film Critics Association story here at Hollywoodnews.com
IRS Form 990 filings list Berlin Entertainment, Inc., receiving a combined $992,270 from the nonprofit between fiscal 2009 and 2011.
Berlin notes that his members seem to be satisfied with the job he's doing since he has been repeatedly re-elected president every two years.
The tax files show that Berlin's base compensation and benefits were a combined $1,297,133 for the three-year period spanning fiscal 2009 to 2011. But his compensation is listed as coming from "related organizations" and not directly from the BFCA, according to the Form 990 documents, which all nonprofits are required to file with the IRS to qualify for tax-exempt status.
When asked about his sizeable compensation, Berlin told HollywoodNews.com in a recent telephone interview: "I did this for five years for zero compensation, but this is what I do all day, every day. I'm really proud of what we've built and accomplished….
"My compensation has grown with the growth of the show," he adds without apology. "The television show is an incredible benefit to the members." He notes that these benefits include such things as access and awards screeners. "That seems a comfortable arrangement for everybody."
One […]
A biopic of R.A. Dickey could be an Oscar winner
As a longtime New York Mets fan, one of my great recent delights in the sporting world was seeing R.A. Dickey come of age while in his thirties. A supposedly washed up pitcher, Dickey developed the knuckleball (a very unique pitch that is almost impossible to master and even harder to hit) and eventually caught on with the Mets, where he went on to dominate for a few years, culminating in winning a Cy Young Award, which is the baseball equivalent of an Oscar win. He wrote a memoir about his life around that time called "Wherever I Wind Up: My Quest for Truth, Authenticity and the Perfect Knuckleball" and a documentary called Knuckleball! was made, centering heavily on his story. Now, he's actually sold the rights to his memoir to actors Logan Marshall-Green and Ben McKenzie, who've started a production company and want to take Dickey's life to the big screen. Friday Night Lights scribe Buzz Bissinger is writing the screenplay, and I personally can't wait to see this flick. Beyond that though, I think that this has a real chance to become an awards vehicle.
The reason that I think Dickey's story is tailor made for Academy voters is the fact that it reads like a movie on its own. He was molested as a child, yet still rose to become a high draft pick, though he lost most of his bonus money and was told his career would never amount to much after doctors found out he was missing an important ligament in his arm. Unfazed, Dickey pressed on, bouncing around the minor leagues and making unremarkable appearances in the bigs, until of course he harnessed the knuckleball. The rest is literally history. That triumphant tale is exactly the sort of one that Oscar voters flock to.
So who could play Dickey? Apparently neither Marshall-Green nor McKenzie are looking to take on the role, so it's going to go out there for some talented actors to circle. While he most closely resembles Timothy Busfield, I don't see that bit of casting coming about. A decade ago, I wouldn't have discounted Kevin Costner playing the role either, but that's also not happening. In my eyes, I actually would like to see Matt Damon potentially play Dickey, but that's just something off of the top of my head. Another top notch pick that actually looks a touch like Dickey in the […]
Oscars®: The Top 25 (Best Original Screenplay)
Continuing a new weekly series I'm doing…we're talking the top 25 Oscar winners in just about every single one of the Academy Award categories. Aside from the shorts and something like Best Sound Mixing like I mentioned previously, I'll be hitting them all over the coming weeks and months, including of course the big eight categories.
Today I'll even knock off the first of those big ones, the ever interesting Best Original Screenplay category. Depending on the category in question, I may wind up discussing the individual winners I'm citing specifically or just giving more of a broad overview of the winners, but for now, I'll still keeping it simple early on. Like I said last week though, in all honesty, you all mostly just want to see the list anyway, so I have no problem obliging you there in that particular regard. All you have to do is just be patient over the next few paragraphs…
Best Original Screenplay is personally one of my favorite Oscar categories, due to the absolute creativity that you can see on display here. Voters sometimes even go out of their comfort zone in honoring scripts written for projects that they'd never touch in the Best Picture category (though that's begun to change a bit). I think you'll be able to see a pattern emerging among my winners, as some of their more out there choices have been my favorites. Maybe that says more about me than it does about members of the Academy, but hey, we should all be thankful that some of these screenplays were able to win those Oscars, as they've inspired countless other writers in the years since.
This week, for this screenplay category, what I'm going to do is give you the list right now, with a few words about each of the top 25 victors that I've chosen. The big eight categories cater to this style nicely, so that's likely how it'll go for all of those. Here we go:
25. American Beauty (Alan Ball) – The film hasn't aged well, but the script itself remains scathingly funny to me. A satire of middle class life and mid life crises, Alan Ball hit on something here, at least at the time. He hasn't been able to get back to that level since then with his work, but man did he deserve the Oscar for this one, no question about that.
24. Pillow […]
Celebs: How Close Is Too Close?
By ROBERT W. WELKOS
Actor Matt Damon enters the green room backstage at NBC's 'The Tonight Show' and is immediately confronted by comedian and fellow Jay Leno guest Larry the Cable Guy wielding a camera phone.
'Okay, everybody, I promised, this is my dressing room after the big 'Tonight' show, Larry the Cable Guy says, and we zip around and there is my new buddy 'go Big Red!' Matty Damon!
'Go Big Red,' a pleasant but subdued Damon replies into the camera.
'My gosh, it's me and Matt!' exclaims the clearly awestruck Larry the Cable Guy.
And who's watching this live video from the green room? Why,anybody anywhere around the world who follows Larry the Cable Guy on a free mobile app called Hang With, or as it's officially known, Hang W/.
Larry the Cable Guy, the blue collar stage persona created by comedian Daniel Lawrence Whitney, clearly relishes bringing his mini-broadcast to his followers on their iPhone, iPad or Android device.
And he's not alone.
Rapper 50 Cent and more than 100 other celebrities and athletes have broadcast live on Hang W/. They include singer Paula Abdul,football great Terrell Owens, comedians Cheech and Chong and Jamie Kennedy, and popular DJ's Chuckie and Kaskade.
Denver Broncos tight end Julius Thomas used Hang W/ at this year's Super Bowl to take his followers on a personal tour inside the Broncos'locker room. 'It's just a little quick behind the scenes view of the locker room,' he tells his viewers. Then zooming in on the gear in his locker, heads, 'It's what it's all about right there.'
Record producer and rapper Timbaland (Timothy Zachery Mosley) even did a broadcast while relaxing at home with his adorable little daughter, Reign, as they sang a short rap tune for the hit maker's fans. Itends with Timbaland asking Reign, 'That's it' That's all we doing ' Say,Goodbye, people.' And she says, 'Goodbye. Love you all.'
So what, exactly, is Hang W/?
It's an app that connects people through live streaming and simultaneous chat, allowing anyone with an iPhone, iPad or Android device tobroadcast live to thousands of viewers. And, it could revolutionize how celebrities connect with their fans, whether it's live streaming themselves at parties,performances, the red carpet or just taking followers along with them on their daily routines.
But celebrities have to be mindful of what information they put out since anyone can […]
Hollywood Film Awards Honors Jerry Weintraub with the "Hollywood Legend Award"
The 17th Annual Hollywood Film Awards announced today that it will be honoring Jerry Weintraub, the prolific and celebrated producer behind the Ocean's Eleven and Karate Kid film series, among others, with the inaugural "Hollywood Legend Award," a special award of merit bestowed to an individual that has made outstanding contributions to film and the entertainment industry.
The announcement was made today by Carlos de Abreu, founder and executive producer of the Hollywood Film Awards. He said, "It is a great honor to recognize Jerry Weintraub, a visionary and true Hollywood legend, with the inaugural 'Hollywood Legend Award'."
The award will be bestowed at the Hollywood Film Awards Gala Ceremony on Monday evening, October 21, 2013 at the Beverly Hilton Hotel in Beverly Hills.
This year Weintraub produced the HBO film Behind the Candelabra, starring Matt Damon and Michael Douglas, which was nominated for an impressive 13 Emmy Awards. His career includes promoting Elvis Presley, Frank Sinatra, Bob Dylan, and Led Zeppelin and managing such talents as John Denver, The Carpenters and The Moody Blues among others. His remarkable list of film hits include Nashville, Diner, The Karate Kid, and the Ocean's Eleven series.
The Hollywood Film Awards honors cherished stars, filmmakers and up-and-coming talent, and traditionally kicks off the film awards season with the biggest stars and top industry executives in attendance.
"We are very proud to be the first stop of the awards season. In the last ten years, a total of 96 Oscar® nominations and 34 Oscars® were given to the honorees of the Hollywood Film Awards," said de Abreu.
Last year's awards show reached a total TV audience of more than 41 million media impressions, in addition to more than 300 million online and print readers' impressions.
Founded in 1957, dick clark productions, Inc (DCP) is a leading independent producer of television programming. DCP produces perennial hits such as the "American Music Awards," "Golden Globe Awards," "Academy of Country Music Awards," and "Dick Clark's New Year's Rockin' Eve with Ryan Seacrest." DCP also produces popular weekly television programming, including "So You Think You Can Dance," and owns and maintains one of the world's most unique and extensive entertainment libraries, which includes more than 30 years of "American Bandstand" footage. For additional information, visit www.dickclark.com.
The Hollywood Film Awards® founded in 1997 were created to celebrate Hollywood and launch the awards season. The recipients of […]
Ben Affleck, Matt Damon reunite to allegedly plan movie
HollywoodNews.com: Although their lives have been busy with plenty of work and kids, it looks like Ben Affleck and Matt Damon have found some time to reunite.
The best friends were reportedly spotted attending a meeting together in Santa Monica where they were allegedly talking about a new project, states UsMagazine.com. It is rumored they are working on bringing the story of mobster Whitey Bulger to the big screen.
Affleck will reportedly direct while Damon is allegedly scheduled to star in the film.
The two haven't worked together recently but have remained close friends all these years.
Are you looking forward to them working together?
Follow Hollywood News on Twitter for up-to-date news information.
Hollywood News, Hollywood Awards, Awards, Movies, News, Award News, Breaking News, Entertainment News, Movie News, Music News
Image by PR Photos


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Nesby Phips
Song & Album Downloads
Bakadahouse Music LLC
Featuring: Curren$y
Song: Rubenstein Bros.
Nesby Phips is sure moving up in the world; just the other day, the New Orleans spitter sat down for brunch with the Rubenstein Bros., proprietors of one of his city's most dinstinguished... More
Posted 3 years ago
New Orleans up-and-comer Nesby Phips has joined forces with Bakadahouse Music and The DJBooth to bring fans his new mixtape, The Lockout. The latest entry in the artist's Phiptapes series, the project features 13 fresh... More
Posted 3 years ago
Song: Hiding In Plain Sight
If you haven't noticed Nesby Phips around the Booth lately, you haven't been looking hard enough; as the N.O. spitter puts it on the latest leak off his forthcoming, DJBooth-sponsored... More
Posted 3 years ago
Song: H.D. & B.G.
As they say, "I ain't got nothing but some hard d*ck and bubble gum, and I'm fresh out of bubble gum." Actually, I'm not sure who says that; the only person I know for... More
Posted 3 years ago
Song: Changes
New Orleans producer, emcee and engineer Nesby Phips has never been one to stand still, hence his new release Changes in support of his forthcoming Life With Options project. The song, which was... More
Posted about 4 years ago
Song: Magic
Hoping to bring our readers under his spell, Dirty Southern up-and-comer Nesby Phips has stepped into the Booth to work a little Magic on your eardrums. On this unreleased joint, the pensive, sampled... More
Posted 4 years ago
Sample Text - Sample Link


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Daemen College : Offices & Services : Student Success Center : Academic Advisement : Student Resources : Testing Tips
Academic Advisement
Research & Information Commons
Phone 839-8228
That's the best way to really get a feel for what Daemen is all about!
Daemen is located in suburban Amherst, New York just minutes away from
the city of Buffalo. To schedule a weekday individual interview and
tour the campus, email us firstname.lastname@example.com or call 800.462.7652.
Daemen Professors & Students from several departments work at a public clinic to provide free medical care in Progreso Dos in the Dominican Republic during the semester break.
"I think one of the most crucial aspects of the Daemen curriculum is the required internship. I was an accounting intern for both a private health care company and a certified public accounting firm. I was able to apply lessons learned in the classroom to real business situations. When my internships were over, each company offered me a full-time position."
Memorial Giving
A memorial gift is a gift that takes on special meaning and memorial givers enjoy knowing their actions will live on for future generations.
©2014 Daemen College : 4380 Main Street, Amherst, NY 14226 : 800.462.7652


text:
And no, I'm not talking about "giving the whole load." Earlier this week Vice President Joe Biden had an epic day of failure on the campaign trail. First, he said "I'm going to give you the whole load" and then he gave a shout out to the Cleveland Plain Dealer, an Ohio newspaper, saying it was the best "in the state while he was campaigning in Florida.
Thursday, when campaigning for Obama in Ohio, Bill Clinton pulled a Biden by saying he was honored to "be in Pennsylvania." The crowd can be heard shouting, "Ohio! Ohio!"
|Katie Pavlich is the Editor at Townhall.com. Follow her on Twitter @katiepavlich. She is a New York Times Best Selling author. Her new book Assault and Flattery: The Truth About the Left and Their War on Women, will be published on July 8, 2014.
"Mamas, don't let your babies grow up to be brainwashed government dependents. Buy this book!" says Michelle Malkin.
"This is a thorough and gutsy book that should help set history straight." - Mark Levin
Buy Katie's book today and help us keep the pressure on the Left.
Author Photo credit: Jensen Sutta Photography


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1. Preheat the oven at 400°F.
2. Lightly flour the kitchen counter and unfold one pastry sheet. Roll the pastry sheet into an 18x9-inch rectangle. Cut the pastry into 18 (3-inch) squares.
3. Place the pastry squares into the muffin-pan cups.
4. Spoon one teaspoon of peanut butter into each pastry cup. Top each with a square of chocolate and 3 mini-marshmallows.
5. Bake in oven for 10 minutes until the pastries are golden brown.
6. Place the pastry on a wire rack along with the pan for 10 minutes.
7. Serve Chocolate Peanut Butter Pastry Cups with fruit juice.


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Nats reliever Ryan Mattheus breaks his hand punching a locker
May 21, 2013, 9:15 AM EDT
Some guys have 80 velocity, some guys have 80 movement. Some guys have 80 lack of impulse control:
Ryan Mattheus will be placed on the disabled list after breaking his right hand Sunday in San Diego, an injury that didn't occur during the Nationals' 13-4 loss to the Padres but afterward when the right-hander punched his locker in frustration.
We get one of these every year, it seems. The "punched a locker" thing is always interesting to me. Almost all major league clubhouses have open lockers with, usually, wood sides to them. So while you can picture it as a guy in a high school hallway punching a flimsy piece of aluminum which gives a bit, he's basically just punching a solid wood wall. Which is S-M-R-T.
The Nats have lost five of seven and have a seriously depleted bullpen at the moment. Stephen Strasburg goes for Washington tonight. The game is only scheduled for nine innings, but it'd be really helpful if he'd give them about 16 this evening.
- albertmn - May 21, 2013 at 9:37 AM
Didn't he ever watch Bull Durham? You don't punch with your pitching hand.
- DelawarePhilliesFan - May 21, 2013 at 9:47 AM
Ryan Madson did that when he was on the Phillies.
Ryan Mattheus has done the same.
Any other Ryan M's out there?
- kopy - May 21, 2013 at 10:15 AM
I typed "Ryan M" into the Google machine and "Ryan McDonagh" was the top option. He went to the same fancy pants high school as Joe Mauer and Paul Molitor so maybe he broke his hand slamming his golf club when his caddy wouldn't do it for him or something.
- missthemexpos - May 21, 2013 at 9:50 AM
Time for teams to start installing punching bags in the locker rooms.
- proudlycanadian - May 21, 2013 at 10:07 AM
I would exclude the Marlins and the Astros because they already are punching bags.
- natslady - May 21, 2013 at 12:17 PM
We have one, it's in the hallway specifically for Harper's use. Unfortunately, the team was in San Diego–the Padres are such inconsiderate hosts, really.
- brewcrewfan54 - May 21, 2013 at 10:20 AM
That's some Natitude!
- goskinsvt - May 21, 2013 at 10:27 AM
I feel like every park the Nats play in should be padded with something everywhere. They seem to hurt themselves a lot on hard objects (non-padded walls, lockers, etc.)
- digbysellers - May 21, 2013 at 10:58 AM
He's gritty and should be traded to the D'backs immediately.
- faulkner22 - May 21, 2013 at 11:00 AM
That's a clown move, bro.
- evanwins - May 21, 2013 at 11:27 AM
As a Phillies fan I can say that this sucks. This guy was great for opposing teams. Make it back soon.
In other Nati-News, Desmond and Strasburg still not impressed. Go Nats, the greatest team in the history of baseball hype. Keep playing and keep showing the media exactly how much they don't know. Keep up the proof that these "experts" are as bad at their jobs as Angel Hernandez.
- natslady - May 21, 2013 at 12:32 PM
If I were you, I'd pay attention to my own house. Colbert's got his diva on again, I see, and of the thirteen games the Fish have won, four over the Fillies?
- natslady - May 21, 2013 at 12:35 PM
BTW, it wasn't Desi who said that, he's got better sense. It was Espinosa… And I don't blame him and Stras, they stick up for their teammates, which is more than can be said for Colbert. See you this weekend. Without Werth, unfortunately.
- DelawarePhilliesFan - May 21, 2013 at 2:05 PM
A) Who is Colbert? (seriously, I am stumped who you could be refering to)
B) Providing bulletin board material is not the same as "sticking up for your teamates"
- natslady - May 21, 2013 at 6:59 PM
Sorry, I was working and didn't see your inquiry. "Colbert" is Cole Hamels' given name. So Colbert is what we call him in Nats Town. I understand you call him "Hollywood."
- evanwins - May 22, 2013 at 1:31 PM
Saying "I'm not impressed" about another team is not sticking up for YOUR team it's taking a dig at another.
Get your head out of your ass.
And I don't care it The Phillies lose to the Marlins or The AL All Star team, a loss is a loss. It just so happens that they ust went 7-5 through a stretch mostly on the road against all 1st and 2nd place teams.
And regarding Colbert? He'll be fine.
- natslady - May 21, 2013 at 12:27 PM
Nats fans (not me, in particular, but some on the Nats blogs) were clamoring for Moves To Be Made. Well, thanks to Mr. Mattheus, Moves Are Being Made.
Eury Perez sent down for no crime other than not being able to hit major-league pitching (in which he is far from alone on this team right now).
Fernando Abad (LHP) brought up for short relief (replacing Mattheus).
Yunesky Maya (RHP) brought up for maybe three days while Stammen gets rested.
Zach Duke not DFA, can't explain that one, except maybe he will pinch hit from now on…
- natslady - May 21, 2013 at 12:51 PM
For Nats fans who don't know Abad, here is what he did in spring training.
- Stiller43 - May 21, 2013 at 1:00 PM
I read that headline real quick and thought it said "hooker"….
Probably would have led to a better story
- scoocha - May 21, 2013 at 1:01 PM
And he probably still receives 100% of his salary. Only in the MLB. So ridiculous.
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Periodontal Disease Linked To Other Diseases?
Periodontal disease, comes in two forms gingivitis (reversible) and periodontitis (treatable but non reversible), is an infection of the gums caused by bacteria found in
plaque. Recent studies have shown between 50-75% of people have some form of periodontal disease. More statistics show that approximately 30% of Americans are at an increased risk of developing periodontal disease due to genetic factors. These statistics show how much a health concern periodontal disease can be especially when you add in even more research showing periodontal disease links with systemic diseases.
Factors in Development of Periodontal Disease
-Poor Oral Hygiene.
-Tobacco Use.
-Teeth Grinding or Bruxism.
-Poor Immune System
-Systemic disease.
The earliest and mildest stage of gum disease is gingivitis, where the gums redden and bleed easily. If not treated, inflammation of the tissue occurs, resulting in progression of the disease to periodontitis. Gingivitis is characterized by receding gums, loose teeth, sores, sensitive gums, swollen gums, red or discolored gums, chronic bad breath, change in teeth alignment and teeth movement. The ultimate consequence of advanced periodontal disease is loss of teeth, which occurs when the tissue and bone supporting the tooth breaks down.
Periodontal disease was previously thought to affect only the teeth and gums, but researchers have discovered that periodontal disease influences the overall health and well-being of an individual. Research has shown that gum disease is a risk factor for many health conditions throughout the body. The gum disease causing bacteria that normally resides around the teeth can enter the blood stream and reach other organs and tissues in the body. Once there, the bacteria release disease-causing agents that can lead to chronic inflammatory conditions that can include:
Diabetes Mellitus (or simply Diabetes)
Periodontal disease impairs the body's ability to maintain blood sugar levels making you more prone to diabetes or making diabetic symptoms worse. On the other hand, diabetic patients are more likely to suffer from periodontal disease due to a weakened immune system, making it easier for them to catch infections, viruses, and exhibit delayed wound healing.
According to scientific studies, gum disease increases the risk of stroke and coronary artery disease. A chronic infection of the gums can be directly related to an increased risk of reduced blood flow to the brain. Stroke and gum disease have similar risk factors and severe inflammation from periodontal disease increases the risks of having a stroke.
Heart Disease
Having periodontal disease puts you at higher risks of heart disease. Just like periodontal disease, heart disease is a chronic inflammatory disease which can be greatly impacted by periodontal disease. The more severe the periodontal infection, the higher the risk of developing heart conditions such as high blood pressure, high cholesterol and acute coronary syndrome.
Respiratory Infections
When the bacteria in the plaque that causes gum disease goes to the lungs, it can cause respiratory diseases such as pneumonia. This explains the increased cases of pneumonia and other respiratory conditions in people with periodontal disease. This also is in conjunction with patients with lowered immune systems which makes it easier for them to be susceptible to these bacterial attacks.
After considering risk factors for cancer including age, diabetes, smoking, BMI and more, experts found periodontal disease as a risk factor for lung, kidney, pancreatic, head, neck and hematologic cancers. Inflammation caused by periodontal disease is a major contributing factor to these cancers.
Bad Breath (Halitosis)
Periodontal disease can result in chronic bad breath which is more of a social issue than a medical one. Bacteria deposits on the tongue can also cause bad breath. This is one of the few conditions caused by periodontal disease that can be treated at home by practicing proper dental care to control halitosis (brushing, flossing, mouthrinses, tongue scraping).
Complications with Birth and Pregnancy
Periodontal disease in pregnant mothers has been shown to increase the risk of premature delivery and low birth weight. The periodontal bacteria involved cause inflammation of the uterus and cervix. Periodontal disease also increases the risk of developing preeclampsia, a condition characterized by high blood pressure and excess protein.
Rheumatoid Arthritis
Rheumatoid arthritis is a painful chronic inflammatory disease that affects the joints. The relationship between rheumatoid arthritis and periodontal disease goes both ways as each increases inflammation in the other. Patients with rheumatoid arthritis are faced with increased risks and severity of periodontal disease and treating periodontal disease can relieve some of the symptoms of rheumatoid arthritis.
The best and easiest way to prevent and control periodontal disease is by maintaining a good oral hygiene program which includes regular dental visits as well as diligent at home care. This includes brushing, flossing, use of mouth rinse, and tongue scraping. Your health is important, taking care of your dental health is a great way to start taking care of your overall health.
© 2013, Marielaina Perrone DDS. All rights reserved. Las Vegas Dental Implants
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Marielaina Perrone DDS is a Family, Cosmetic, & Implant Dentist practicing in Las Vegas and Henderson, Nevada.
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Friday, August 1, 201408/01/2014
Stuff the Bus is This Weekend!
Paul Wappel, Community Relations Director for Horace Mann, joins WTAX's Alex Degman to talk about this weekend's event to benefit Springfield School District 186.
Friday, August 1, 201408/01/2014
Congressman Rodney Davis
Davis joins WTAX's Alex Degman to talk about his vote to sue the president, plus what *didn't* get done ahead of the August recess.
Thursday, July 31, 201407/31/2014
Ward 7 Alderman Joe McMenamin
McMenamin joins WTAX's Alex Degman to talk about underfunded police and fire pension funds and his revised proposal to deal with both of those issues.
Thursday, July 31, 201407/31/2014
U.S. House Votes to Sue President Obama
The House voted to sue the president due, in part, to the perception he's overstepping his bounds and isn't upholding the law.
Wednesday, July 30, 201407/30/2014
Ward 1 Alderman Frank Edwards
Joey talks with Ward 1 Alderman Frank Edwards about the city council meeting last night and decisions on PILOT funds, and the Bel Aire.
Wednesday, July 30, 201407/30/2014
More Sanctions for Russia
The U.S. and the European Union had adopted new and sweeping economic sanctions against Russia for events in the conflict against Ukraine.
Wednesday, July 30, 201407/30/2014
The Gaza Strip
Fifteen people have reportedly died today after a explosions struck a United Nations school being used as a shelter for displace Palestinians. Hamas is blaming Israel and Israel is investigating.
Tuesday, July 29, 201407/29/2014
Keep Kids in School Coalition
Dr. Tracy Milbandt, Division Chief of General Pediatrics at SIU School of Medicine talks with Joey about the effort to make sure kids get physicals and immunizations before school starts.
Tuesday, July 29, 201407/29/2014
Redneck Fishing Tourney
Mike Chrysler joins Joey to talk about the Redneck Fishing Tournament that is coming up in Bath this weekend. Ger more into here – originalrednickfishingtournament.com
Tuesday, July 29, 201407/29/2014
Russia & Malaysian Flight MH17
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What Milwaukee is talking about
Has Kenny Strasser been unmasked?
Mikel Lauber, an anchor/reporter at WSAW in Wausau, has done some detective work on Kenny Strasser, the "yo-yo master" who managed to dupe a group of TV stations, including WSAW, into putting him live on the air.
Lauber received a tip from an Internet radio host in Illinois, who suggested Lauber check out an old website called Cine-Magic.com. If you check the cached versions of the site, you will find a photograph of Mark Proksch, a former University of Wisconsin-Eau Claire student.
Proksch looks remarkably like our friend Kenny Strasser, also known as K-Strass.
In those cached sites, you will also find the name Joe Pickett, who runs a tour called the Found Footage Festival. In our original story on Strasser, Pickett denied there was any connection between his outfit and Strasser, er, Proksch. But Pickett and Proksch apparently were friends at UW-Eau Claire, as was NIck Prueher, another Found Footage staffer.
As Lauber reports, Proksch has done an excellent job at staying out of the limelight since he became an Internet sensation. We stopped by his Milwaukee home on Monday to try to talk to him, but he wasn't home.
His landlord confirmed that Proksch still rents the apartment. When we asked the landlord to ask Proksch to contact Hubbub, the landlord said Proksch was "avoiding us."
In the meantime, you can go to zimzaminc.com and learn more about the legend of Kenny Strasser and Kenny's Corner.
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A Lesson In Communication: What 'New Yorker' Cartoonist Liza Donnelly Can Teach Entrepreneurs
At the edge of what we know is the thing that frightens us. Between what I know and what you know is a universe. It's when we come to the edge of things and then go a little further that we begin to see possibilities we hadn't imagined and opportunities that were hitherto unknown. The edge of things is where we can find the ideas that become the read »Drew Marshall, Contributor


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Rejoice in blessings this holiday
Would you say that you are a thankful person? When someone does something for you or perhaps pays you a compliment, do you thank them? People do many things for me that I can't do for myself, so I am careful to express my appreciation to them for their various acts of kindness.
Living with cerebral palsey has never been easy. For 52 years I have lived with this condition that causes me to be the way that I am. Given my physical condition being what it is, I have to rely on others to help me in ways that I can't help myself. Even though I can't do some things that other people can do, it is an humbling experience that teaches you to be thankful for the people that God sends your way to assist you.
People take so much for granted these days. They need to be more thankful for what they have because life can change so fast. One day you can be on top of the world and the next you can be incapacitated. You just never know. This is the reason that we should always be grateful. This is what Thanksgiving is all about.
In his epistle to the Philippians, the Apostle Paul says, "Be careful for nothing; but in everything by prayer and supplication with thanksgiving let your requests be made known unto God" (4:6). Aren't you glad that we serve a loving God who is concerned with our wishes and wants? This does not mean that He is going to give us everything that we ask Him for. If you are in need of a car and you ask Him for one, He will give you one, although it may not be a Cadillac or a Rolls Royce and there will probably be some work involved on your part.
All too often our prayers are filled with "give me this and give me that" and we fail to give Him thanks for the blessings that He gives us every day. Imagine if your children only came to you when they want something from you. This would not make you feel very good, would it? He says we should make our requests known with thanksgiving. You hear people say things like, "Well … I just don't have much for which to be thankful." I don't see how people can say such things. Go to the hospital, nursing home, or soup kitchen any day of the week and you will find out pretty quickly how thankful you should be.
I love that great old hymn "Count Your Blessings," by Edwin Excell. The refrain of this endearing song says, "Count your blessings, name them one by one; Count you blessings, See what God hath done; Count your blessing, name them one by one; Count your many blessings, see what God hath done."
If people would just sit down and start numbering the things for which they are most grateful, then they might be pleasantly surprise to discover just how rich they really are. Instead, they are busy counting their money, bills, and all the things they wish they had. People are so fixed on all of the problems and cares they have.
We are followers of God and we can find the answers to everything we need are in Him. When are we going to learn that every need that we have comes from Him? We are to rejoice in Him for the ways He has blessed us. The more that we praise Him, the more we are blessed.
Instead, most people sing the "Woe is me" song. Do you know people like this? We all are acquainted with those who think they have it so bad. They have no idea of how blessed they are. But those that have their eyes fixed on the Lord do. They know from where their help comes. He is the source of all blessings and good things.
The Psalmist David wrote in Psalm 95:2, "Let us come before his presence with thanksgiving, and make a joyful noise unto him with psalms." You say, "How do you do this? How can you sing praises to the Lord when you don't feel like it?" There are times when we don't feel much like praising the Lord, but it is times such as these when we need it the most.
Psalm 92:1 reads, "It is a good thing to give thanks unto the LORD, and to sing praises unto your name, O most high." You may say, "I can't sing." This makes no difference, as this is no talent contest! You may be like me and can't carry a tune in a bucket, but it should not hinder us in singing praises unto our God, who is the giver of life and all good things that we have.
I wish you and your family the best Thanksgiving ever! As you gather with family and friends, I pray that you will be mindful of the times, people and things that are most precious to us. I hope you will put away all those petty little things that don't even matter and rejoice in the blessings we have that God has so bountifully bestowed upon us.
Until next week—God bless.


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I organize my day (sometimes the whole week) hour by hour. This helps me stay on track & be sure I get my workouts in
My secret is I added two protein shakes to my plan a day
I drive to work in the mornings with no radio on. It gives me time to reflect and plan my day with out distractions
I have a daily folder for ongoing projects and things I do every day, a weekly folder for things I do once a week, each item marked for their day of the week, and also a monthly folder. Every morning I review all folders and pick the "must do today" items, then the "might do". Then I do 'em!


text: No recent wiki edits to this page. CreationExcaliber was created by Marv Wolfman and Carmine Infantino.Major Story ArcA Sword in HandJason "Slappy" Struthers was a simple, petty thief intent on stealing a sword from the London Museum that was believed to be King Arthur's legendary sword, Excalibur. This sword was however a copy of Excalibur created by the sorceress Morgan Le Fay to use against King Arthur. When Struthers took hold of the sword, he became possessed by one of Morgan's knights. Now calling himself Excaliber, Struthers was used by Morgan to try and steal back her old spell books from Magnus the Magician. Spider-Woman was able to foil Morgan's plan however when she knocked the sword out of Struther's hand, freeing him from Morgan's possession. Struthers was later handed over to SHIELD.Powers and AbilitiesAs Excaliber, Struthers possessed enhanced strength, stamina, reflexes, and durability. He was shown to be unaffected by Spider-Woman's venom blasts, he used his sword to knock a train off its tracks, he was able to turn an ordinary police horse into his personal steed, and he could fire off energy blasts capable of stunning his foes.Struthers only had his powers though as long as he held onto his sword, and would be rendered powerless as soon as he let go of it.


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In his fictitious town of Mountain Center, Donnelly takes inspiration from his hometown of Johnson City to flesh out the places and characters. Johnson Citians will recognize buildings, streets (some real addresses) as well as some of his characters thatComments
For Cindy Hagemann, getting back to public-sector marketing marks a return to one of her passions - helping communities grow.Comments
Comedian Jeanne Robertson Live at Memorial ParkComments
I will always be a green-as-grass novice when it comes to understanding and appreciating all that Asian cuisine has to offer. Growing up, I was fortunate to have parents who liked to dine out, especially at old-school Chinese family style restaurants. TheComments
The 14th Annual East Tennessee Celtic Festival (formerly the Sycamore Shoals Celtic Festival) presented by the Upper East Tennessee Celtic Society will be held this weekend at Sycamore Shoals State Historic Area.Comments
On Saturday at 7:30 p.m., the critically acclaimed Annie Moses Band will perform "Rhapsody In Bluegrass: The Art of American Music" at the Niswonger Performing Arts Center in Greeneville.Comments
Marty McFly had his time traveling DeLorean. Bill & Ted used a time traveling phone booth for their excellent adventures. Theatre Bristol is using music and nostalgia to roll back the clock for the fourth show of their 49th season.Comments
Johnson City Symphony Orchestra will open its 2014-15 season with an outdoor concert Sunday at Boone Lake. "Kinetic Energy," a pops concert, will feature favorites from screen and stage as well as patriotic and light classics. The concert starts at 6 p.m.Comments
President Sabra Hayden wants to be a part of the action that's going on in the city's downtown area, but she knows it's not going to come easily.Comments
John and Angela Vachon, who have purchased over 20 buildings in downtown Kingsport in the past decade, confirm they purchased the theater building last month.Comments


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Back by Popular Demand – The San Pedro Holiday Lighted Boat Parade will be held on Saturday, December 6th starting at 6pm til around 9pm. This traditional event introduces the holiday season and brings us into the holiday mood with a fun event filled with twinkling lights, children's laughter, and Christmas carols. The dazzling boat parade will start at 6pm ... Read More »
Tropic Air Expands
Tropic Air Expands Fleet to 15 aircraft Tropic Air (9N) announces today the addition into scheduled service of another Cessna Grand Caravan turbo prop aircraft. This latest addition to the fleet features air conditioning and the Garmin G1000 glass cockpit avionics suite with radar. This brings the number of Cessna Caravans in its fleet to ten, and overall number of ... Read More »
In prep mode for San Pedro and Belize City
Although it may seem like it, I have not gone MIA (missing in action). I have been super busy with Red Cross stuff in preparation for our community meeting tonight (see details below) and planning for a 3-day volunteer forum in Belize City at Princess Hotel & Casino which is now owned by Ramada. Each branch was invited to send ... Read More »
Belize Association of Houston Family Fun day
It is not uncommon for Belizeans seeking job opportunities to move abroad with the United States and Canada being popular choices and many youth opt to attend universities in other countries as well. According to the 2014 Belize Demographics profile information page, migration continues to transform Belize's population. About 16% of Belizeans live abroad, while immigrants constitute approximately 15% of ... Read More »
Forecast: Busy Weekend in San Pedro
The old saying when it rains it pours is often applicable to our lives in San Pedro. There is always so much going on at once and this weekend is no exception. As much as I wanted to go to trivia last night, I passed in favor of getting extra rest for tomorrow's blood drive hosted as a collaborative effort ... Read More »
Record Breaking Weekend and Belize Weather in November
Everyday choices affect our overall well-being inside and out and lately my choices have affected my sleep. The past week I clocked my average bedtime at 12:37 a.m., nearly two hours later than usual and my average wake time was 6:36 a.m., also on the late side. This was not surprising considering the start of last weekend had my social calendar filled ... Read More »


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26 year old woman | Cucamonga, CA, USA | Seeking men 24-35 within 40 miles of Rancho Cucamonga, CA
In the end, it's not going to matter how many breaths you took, but how many moments took your breath away" - Anonymous
Something's to know about me are that I am a very fun, friendly, and confident woman, who loves beautiful things, to have fun in the moment and am always dreaming bigger! I love to read...I mean really really love to read and barely go a few days without picking up a book.... I think I'm funny and am always up for a good joke. And I appreciate when people feel comfortable enough around me to just say the first thing on their mind. So now to the all important question.....Why am I on Match? Well I'm now looking for a relationship. I am looking for someone outgoing, educated, charismatic, and fun loving. I also want someone who works hard and plays harder. They must also be family oriented, have a great sense of humor, be easy going and goal oriented. I'm not looking to jump into anything without getting to know someone first. I've never been married, so I'm pretty sure it won't kill me to take things slow and make sure we are compatible. I'm unique in that I will not waste time with "shallow" interactions. I'm only open to meaningful, honest, and exclusive relationships with like minded people. I know it's hard to get to know someone by just a few photos and a couple of paragraphs, so if you want to get to know me better you should message me.
Coffee and conversation , Cooking , Dining out , Movies/Videos , Music and concerts , Playing cards , Travel/Sightseeing , Watching sports , Wine tasting
Billiards / Pool, Other types of exercise, Yoga
I exercise 1-2 times per week.
I like Cats, Dogs, Fish
I like them but I don't have any pets. I'm actually afraid of big animals (specifically dogs) but little ones are okay.
Middle of the Road
Boston, Washington D.C., Vegas, Starbucks, anywhere with friends or family..
University of Redlands, Redlands, CA
I love Mexican and Chinese food. Anything spicy is good. My favorite channels are the Food Network, HGTV, and DIY. I like all music for the most part, except for heavy metal and hard rock.
I mostly hang out with my friends and family doing what ever...
What haven't I read?.....recommend something!
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|What she is looking for
|5'5" (165cms)
|5'5" (165cms) to 7'0" (213cms)
|Body type:
|About average, Athletic and toned, Heavyset, A few extra pounds, Stocky
|Blue, Brown, Grey, Green, Hazel
|Dark brown
|Auburn / Red, Black, Light brown, Dark brown, Blonde, Dark blonde, Bald
|No Way
|No Way
|Social Drinker
|Social Drinker, Moderately
|Executive / Management
|No preference
My current job is a mix between educational administration and marketing management.
|I'll tell you later
|No preference
|Never Married
|Never Married, Widow / Widower, Divorced
|Have kids:
|Wants kids:
|Definitely, Someday
|Black / African descent
|Latino / Hispanic, White / Caucasian
I have a very diverse family and have been fortunate enough to learn and interact with people of different backgrounds.
|Christian / Other
|Christian / Catholic, Christian / LDS, Christian / Protestant, Spiritual but not religious, Other, Christian / Other
I was raised Christian but very non-traditional. I respect all faiths and think that as long as you are at peace with your spirituality then that's the right one for you.
|Graduate degree
|Bachelors degree, Graduate degree, PhD / Post Doctoral
I have a B.A in Management and an MBA in Finance.


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