text:
The invention is directed to a method for the prophylaxis or treatment of an animal for deleterious physiological effects such as systemic hypotension caused by nitric oxide production induced by a biological response modifier. Examples of such biological response modifiers include but are not limited to a cytokine and an endotoxin. The invention is also directed to a method for the treatment of septic shock.
1. A method for alleviating deleterious nitric-oxide induced effects in an animal, the method comprising administering a therapeutically effective amount of an iron hemoprotein to said animal.
2. A method of reducing nitric oxide levels induced in an animal by a cytokine or by endotoxin, the method comprising administering to said animal an iron hemoprotein in an amount effective to bind or oxidize nitric oxide.
3. A method for treatment of systemic hypotension in a patient induced by chemotherapeutic treatment with a cytokine, the method comprising administering a therapeutically effective amount of an iron hemoprotein to said patient.
4. A method for treatment of systemic hypotension in a septic patient caused by endotoxin-induced nitric oxide production comprising intravascularly administering an amount of an iron hemoprotein sufficient to bind or oxidize substantially all of the nitric oxide produced.
5. The method of claim 1, 2, 3 or 4 where the iron hemoprotein is hemoglobin or myoglobin.
6. The method of claim 1, 2, 3 or 4 where the amount is from 0.1 to 10 g/kg body weight.
7. The method of claim 1, 2, or 3 wherein the administering is intravascular.
8. The method of claim 1 where the deleterious nitric oxide-induced effect is systemic hypotension.
9. A method for treatment of an animal for systemic hypotension caused by nitric oxide comprising administering a therapeutically effective amount of hemoglobin.
10. The method of claim 9 where the nitric oxide is induced by a cytokine.
11. The method according to claim 10 where the cytokine is tumor necrosis factor, interleukin-1, or interleukin-2.
12. A method for thee treatment of an animal for systemic hypotension caused by endotoxin-induced nitric oxide production comprising administering a therapeutically effective amount of hemoglobin.
13. A method for treatment of systemic hypotension in a patient caused by nitric oxide production induced by a cytokine selected from the group consisting of tumor necrosis factor, interleukin-1, and interleukin-2 comprising administering an amount of hemoglobin sufficient to bind or oxidize substantially all of the nitric oxide produced.
14. The method of claim 9, 12 or 13 where the amount is from 0.1 to 10 g/kg body weight.
15. The method of claim 9, 12 or 13 where the administering is intravascular.
The invention is directed to a method for the prophylaxis or treatment of an animal for systemic hypotension induced by a biological response modifier. Examples of such biological response modifiers include but are not limited to a cytokine and an endotoxin. The invention is also directed to a method for the treatment of septic shock.
Endothelial cells have been shown to produce a potent vasodilator known as Endothelium-Derived Relaxing Factor (EDRF). Many naturally occurring substances which act as physiological vasodilators mediate all or part of their action by stimulating the release of EDRF. Examples of such substances include acetylcholine, histamine, bradykinin, leukotrienes, ADP, and ATP. Recent studies have identified EDRF as nitric oxide, a short lived, unstable compound (Ignarro et al., 1987, Proc. Natl. Acad. Sci. U.S.A. 84:9265-9269 and Palmer et al., 1987, Nature 327:524-526).
L-Arginine is the metabolic precursor of EDRF (Schmidt et al., 1988, Eur. J. Pharmacol. 154:213-216). NG -methyl-L-arginine is a competitive inhibitor of the biosynthetic pathway of EDRF (Palmer et al., 1988, Nature 333:664-666). Administration of NG -methyl-L-arginine to guinea pigs and rabbits has been shown to increase blood pressure (Aisaka et al., 1989, Biochem. Biophys. Res. Commun. 160:881-886). Nitric oxide (NO) appears to be synthesized from L-arginine by the enzyme, NO synthase; the coproduct is L-citrulline (Moncada et al., 1991, J Cardiovascular Pharmacol. 17 (Suppl. 3):S1-S9). NO is an endogenous stimulator for soluble guanylate cyclase.
Nitric oxide has been found to be produced by macrophages, endothelial cells, neutrophils, Kupffer cells and hepatocytes, murine fibroblasts stimulated with cytokines, and EMT-6 cells, a spontaneous reurine mammary adenocarcinoma cell line when treated with cytokine (reviewed in Moncada et al., 1991, Pharmacol. Reviews 43:109-142). Specifically, macrophage cells become activated by 12-36 hour treatments with gamma-interferon, bacterial endotoxin and various cytokines (reviewed in Collier and Vallance, 1989, Trends in Pharmacol. Sci. 10:427-431).
Endothelial cells in the presence of gamma-interferon, have been found to secrete large quantities of arginine-derived nitrogen oxides after activation by tumor necrosis factor (TNF) or endotoxin (Kilbourn and Belloni, 1990, J. Natl. Cancer Inst. 82:772-776). TNF causes marked hypotension in mammals (Tracey et al., 1986, Gynecol. Obstet. 164:415-422; Old, 1985, Science 230:630-632). Additionally, TNF is thought to mediate the vascular collapse resulting from bacterial endotoxin (Beutler et al., 1985, Science 229:869-871). It has recently been shown that arginine derivatives inhibit systemic hypotension associated with nitric oxide production, specifically treatment with TNF, gamma interferon, interleukin-2, and bacterial endotoxin (Kilbourn et al., U.S. Pat. No. 5,028,627, issued Jul. 2, 1991; PCT lypplication no. WO 91/04023, published Apr. 4, 1991; and Kilbourn et al., 1990, Proc. Natl. Acad. Sci. U.S.A. 87:3629-3632). Nitric oxide overproduction is also thought to be involved in numerous other pathogenic or potentially pathogenic syndromes. For example, some of these syndromes are thought to be associated with malaria, senescence and diabetes. The procedures of the present invention may also be used to prevent, inhibit and/or alleviate such NO-related syndromes.
Nitric oxide reacts with hemoglobin to form nitrosylhemoglobin (Kosaka et al., 1989, Free Radical Biology 7:653-658). Nitrosylhemoglobin reacts with oxygen to yield nitrate and methemoglobin, which is rapidly reduced by methemoglobin reductase. At least part of the nitric oxide is oxidized by oxygen to NO2, which is in turn converted to nitrite and nitrate.
The formation of nitrosylhemoglobin has been used to quantify nitric oxide present in mice given bacterial endotoxin, specifically by using electron paramagnetic resonance (EPR) spectroscopy to detect NO liganded to hemoglobin (Wang et al., 1991, Life Sciences 49:55-60). Although bacterial endotoxin induces septic shock, hypotension was not observed in the Wang et al. study. Those skilled in the art recognize that such EPR studies may be used to quantitate the binding of NO to other hemoproteins as well.
Hemoglobin has also been found to inhibit nonvascular relaxant responses to EDRF (Buga et al., 1989, Eur. J. Pharmacol. 161:61-72). Furthermore, hemoglobin at 1 μM reduced and at 10 μM abolished tie endothelium-dependent relaxation induced by acetylcholine or by A23187 in rabbit aortic rings (Martin et al., 1985, J. Pharmacol. Exp. Ther. 232:708-716). It was hypothesized by Martin et al. that the hemoglobin inhibits endothelium-dependent induced relaxation by binding nitric oxide.
Septic shock is characterized by inadequate tissue perfusion and is usually caused by gram-negative enteric bacilli such as Escherichia coli, Klebsiella, Enterobacter, Pseudomanas, Serratia, and Bacteroides (see Harrison's INTERNAL MEDICINE, 10th Ed., vol.1, Petersdorf et al., eds., McGraw Hill, N.Y. 1983). Gram-negative bacilli possess endotoxin, also known as lipopolysaccharide (LPS), which is a cell-wall component that can activate leukocytes in minute amounts in the blood.
Septic shock is characterized by chills, fever, nausea, vomiting, diarrhea, and prostration. The subsequent development of septic shock is characterized by tachycardia, tachypnea, hypotension, peripheral cyanosis, mental obtundation, and oliguria. As shock progresses, oliguria persists, and heart failure, respiratory insufficiency, and coma supervene. Death usually results from pulmonary edema, generalized anoxemia secondary to respiratory insufficiency, cardiac arrhythmia, disseminated intravascular coagulation with bleeding, cerebral anoxia, or a combination of the above.
Most of the damage caused by septic shock is thought to be caused by endotoxin. It has also been hypothesized that nitric oxide plays a major role in effecting hypotension in those exposed to endotoxin (Kilbourn et al. Biochem. and Biophys. Res. Comm. 1991, vol. 172:1132-1138). Studies have shown that the hypotension and loss of vascular responsiveness resulting from endotoxin administration is reversed by the administration of analogues of L-arginine which inhibit nitric oxide production (Parratt and Stoclet, 1991, Applied Cardiopulmonary Pathophysiology 4:143-149; and Kilbourn et al., 1990, Proc. Natl. Acad. Sci. U.S.A. 87:3629-3632).
In certain cases, acute blood loss may cause hypotension. After initial recovery due to standard hypotension treatment, a breach of intestinal integrity may subsequently cause septic hypotension. Such a syndrome is readily treated according to the methods of the present invention.
There are currently a number of clinical procedures available for the treatment of septic shock (reviewed in Harrison's INTERNAL MEDICINE, 10th Ed., vol.1, Petersdorf et al., eds., McGraw Hill, N.Y. 1983). However each has its drawbacks. One treatment involves the replacement of blood volume with blood, plasma, or other fluids such as human serum albumin in appropriate electrolyte solutions such as dextrose saline and bicarbonate. However, large quantities of these substances are required and may amount to 8 to 12 liters in only a few hours, leading to massive shifts in fluid and electrolyte balances.
Antibiotics may also be used to treat septic shock. However, to determine the appropriate antibiotics to use, blood cultures must be taken and evaluated. Such cultures take time. Additionally, antibiotics may initially worsen shock caused by bacteria since they kill the bacteria and the dying organisms can release even more lipopolysaccharide into the body.
Vasoactive drugs such as beta-receptor stimulants (e.g. isoproterenol and dopamine) and alpha-receptor blocking agents (phenoxybenzamine and phentolamine) have also been therapeutically used since septic shock is accompanied by maximal stimulation of alpha-adrenergic receptors. However, all of these drugs may have serious side effects. In addition, studies by Stoclet et al. (Amer. J. Physiol. 259:H1038-1043) suggest that endotoxic shock and NO production can cause lowered sensitivity to these agents, decreasing their effectiveness. The iron hemoprotein therapy of the present invention may be used to reverse this loss of sensitivity.
Another treatment approach involves using human antiserum to E. coli J5, a mutant strain that produces endotoxin without the variable oligosaccharide side chains. However, since such antiserum comes from many different human donors with varying immune responses to the antigen, it cannot be rigidly standardized for effectiveness. Such antiserum may also transmit infectious agents. To circumvent these problems, monoclonal antibodies that bind to endotoxin are currently being tested in patients suffering from septic shock (reviewed in Johnston, 1991, J. NIH Res. 3:61-65). A disadvantage of this approach is that treatment of septic patients with antiendotoxin antibodies has no immediate effect on blood pressure and may not work at all if patients are not treated at an early stage.
Another approach used to treat septic shock involves administering bactericidal permeability-increasing protein (BPI), a human protein derived from neutrophil granules. This protein binds to LPS. Versions of the molecule are currently being developed (reviewed in Johnston, 1991, J. NIH Res. 3:61-65).
Approaches are also being developed for treating septic shock by preventing endotoxin from activating leukocytes that start the inflammatory response (reviewed in Johnston, 1991, J. NIH Res. 3:61-65). Examples of such approaches include administering a soluble interleukin-1 (IL-1) receptor, an IL-1 receptor antagonist, a monoclonal antibody to TNF-α, soluble TNF receptor and a monoclonal antibody to TNF-α receptor. All of these approaches suffer from the fact that blockade of the cytokine receptor interaction does not have an immediate effect on nitric oxide production by NO synthase (i.e.--once induced by cytokines, NO synthase continue to produce NO even after removal of the stimulus).
The present invention involves the treatment of a nitric oxide induced malady such as hypotension by administration of an iron hemoprotein which has substantial affinity for nitric oxide and/or catalyzes nitric oxide oxidation to nitrates or nitrites. The most preferred iron hemoprotein is hemoglobin, particularly recombinant hemoglobin. Myoglobin, which also is known to bind nitric oxide may also be utilized. Additional iron hemoproteins, including certain cytochromes, e.g. are known and may be used when demonstrating the above mentioned nitric oxide-related effects. Particularly preferred hemoglobins are those which have a long circulating lifetime, e.g., those which have a poor affinity for haptoglobin. Those skilled in the art will recognize that any iron heme-containing proteins or peptides binding NO may be administered to an animal in a pharmaceutically effective amount to remove nitric oxide and prevent or treat hypotension or other NO-induced deleterious effects.
Despite previous results showing the interaction of iron hemoproteins such as hemoglobin with nitric oxide, the therapeutically effective results of the present inventive method are quite surprising and unexpected. Such a result could not have been expected in view of the fact that normal mammalian blood fluid is replete with hemoglobin contained in red blood cells which contain about 34% hemoglobin. Such cells usually constitute over 43% of the blood. Thus blood is about 15% hemoglobin. Additionally, nitric oxide is quite lipophilic and readily penetrates red blood cells. One skilled in the art would have expected that any effects of a free iron hemoprotein such as hemoglobin in the blood to remove deleterious nitric oxide would have been insignificant as compared to the effects of hemoglobin contained in red blood cells already present. The basis for the surprising and unexpectedly effective results of the present invention are incompletely understood at the present time, but, as the present invention is described, it will be clear to those of skill in the art that the free iron hemoproteins of the present invention such as hemoglobin are phenomenally effective for therapeutic purposes related to a reversal of deleterious physiological effects induced by nitric oxide in vivo.
The invention is directed to a method for the prophylaxis or treatment of an animal for systemic hypotension or other pathogenic syndromes induced by inappropriate nitric oxide production. Such hypotension may be induced by certain cytokines or a microbial toxin such as bacterial endotoxin. The method involves administering a therapeutically effective amount of an iron hemoprotein such as hemoglobin and the like. The administration is preferably intravascular, which includes intraarterial and intravenous. Other parenteral administration routes, e.g. peritoneal or intralymphatic, to name but a few, can be used for removal of undesired NO production at related sites. Enteral administration could be feasible if the hemoprotein or a derivative thereof was resistant to digestion and passed into the circulation. The animal may be, for example, a domestic mammal or a human patient. The systemic hypotension most characteristically results from abnormal production of nitric oxide induced by a cytokine or microbial toxin. A therapeutically effective amount of iron hemoprotein is an amount sufficient to bind and/or oxidize substantially all of the free nitric oxide and thus removing it from the circulation. In a preferred embodiment, the iron hemoprotein is hemoglobin or myoglobin and is administered intravascularly.
Bacterial endotoxin is a primary causative agent of septic shock. Elevated levels of nitric oxide have been found in animals in septic shock (Wang et al., 1991, Life Sciences 49:55-60). Therefore, the invention is also directed to a method for the treatment of septic shock in a patient comprising administering a therapeutically effective amount of an iron hemoprotein such as the preferred hemoglobin.
FIG. 1 shows the effect of hemoglobin on endotoxin-induced hypotension in a dog. The endotoxin-induced mean arterial decline is interrupted by administration of hemoglobin.
In a major embodiment, the present invention is directed to a method for the prophylaxis or treatment of an animal for systemic hypotension induced by a biological response modifier directly or indirectly causing nitric oxide overproduction. Examples of such biological response modifiers include but are not limited to cytokines. The invention is also directed to a method for the treatment of septic shock and associated hypotension.
The starting material, unmodified hemoglobin, may be obtained using procedures known in the art (see for example, PCT Application Publication No. WO 88/03408, published May 19, 1988; U.S. Pat. No. 4,001,401; Feola et al., 1983, Surgery Gynecology and Obstetrics 157:399-408; De Venuto et al., 1979, Surgery Gynecology and Obstetrics 149:417-436). For example, unmodified stroma-free hemoglobin may be obtained as follows: (a) obtaining whole blood; (b) separating red blood cells from other components of whole blood; (c) isolating the hemoglobin from the erythrocytes; and (d) separating the hemoglobin from stroma and other impurities.
Stroma-free hemoglobin can be prepared by starting with erythrocytes in freshly drawn, outdated, or frozen packed cells or whole blood. The blood should be drawn in a sterile fashion into containers with sufficient anticoagulant activity to prevent clot formation.
In one embodiment, blood cells are washed in a saline solution and centrifuged to separate red blood cells from white blood cells as well as to additionally remove free proteins (Feola et al., 1983, Surgery Gynecology and Obstetrics 157:399-408). In another embodiment, the red cells may be separated from other blood cells by passing through a semi-continuous type centrifuge as described in PCT Application Publication No. WO 88/03408, published May 19, 1988.
Hemoglobin may be isolated in one embodiment by diluting the red blood cell solution in water or an organic solvent at 2�-100� C. to separate the hemoglobin in red blood cells from all cell debris (PCT Application Publication No. WO 88/03408, published May 19, 1988; U.S. Pat. No. 4,001,401; Feola et al., 1983, Surgery Gynecology and Obstetrics 157:399-408). In another embodiment, the hemoglobin is precipitated as a zinc complex by the addition of a zinc salt to a hemoglobin solution (De Venuto et al., 1979, Surgery Gynecology and Obstetrics 149:417-436).
The isolated hemoglobin, in another embodiment, may be purified by ultrafiltration through for example a 0.5 micron filter which retains the cellular components and passes the hemoglobin.
Hemoglobin may also be obtained through other procedures known in the art. For example, bacterial strains (see for example Nagai and Hoffman, U.S. Pat. No. 5,028,588, issued Jul. 2, 1991) or yeast (see for example PCT Application Publication No. W090/13645, published Nov. 15, 1990), or other eukaryotic organisms may be engineered to produce hemoglobin by recombinant DNA techniques. Recombinant hemoglobin is thought to be particularly advantageous because it can be readily produced in large quantities and because utilization of such recombinant hemoglobin obviates the possibility of blood-borne infection such as that by various viruses or retroviruses.
The hemoglobin may be for example, any human hemoglobin, including but not limited to HbA (alpha2 beta2), HbA2 (alpha2 delta2), HbF (alpha2 gamma2), Hb Barts (gamma4), HbH (beta4), and Hb Portland I (zeta2 gamma2), Hb Portland II (zeta2 beta2), Hb Portland III (zeta2 delta2) Hb Gower I (zeta2 epsilon2), and Hb Gower II (alpha2 epsilon2); as well as any other animal hemoglobin, e.g. bovine or porcine hemoglobin.
The hemoglobin used in the method of the present invention may be chemically modified using procedures known in the art to increase tetramer stability and/or lower oxygen affinity. Examples of chemical modifications to increase the tetramer stability include but are not limited to crosslinking with polyalkylene glycol (Iwashita, U.S. Pat. Nos. 4,412,989 and 4,301,144), with polyalkylene oxide (Iwasake, U.S. Pat. No. 4,670,417); with a polysaccharide (Nicolau, U.S. Pat. Nos. 4,321,259 and 4,473,563); with inositol phosphate (Wong, U.S. Pat. Nos. 4,710,488 and 4,650,786); with a bifunctional crosslinking agent (Morris et al., U.S. Pat. No. 4,061,736); with insulin (Ajisaka, U.S. Pat. No. 4,377,512); and with a crosslinking agent so that the hemoglobin composition is intramolecularly crosslinked between lys 99 alpha1 and lys 99 alpha2 (Walder, U.S. Pat. No. 4,598,064). Examples of chemical modifications to decrease the oxygen affinity of isolated hemoglobin include but are not limited to polymerization with pyridoxal phosphate (Sehgal et al., 1984, Surgery, 95:433-438) and using reagents that mimic 2,3-DPG (Bucci et al., U.S. Pat. No. 4,584,130).
In a further embodiment, the hemoglobin used in the method of the present invention may be a hemoglobin variant, a hemoglobin comprising a globin chain from a nucleotide sequence which has been altered in such a fashion so as to result in the alteration of the structure or function of the hemoglobin, but so that the hemoglobin still remains functionally active as defined by the ability to reversibly bind to oxygen (or nitric oxide, of course). Categories of hemoglobin variants include but are not limited to variants which autopolymerize; variants in which the tetramer does not dissociate under physiological conditions i-n vivo (e.g. Hb Rainier, beta-145 tyrosine is replaced by cysteine); variants with lowered intrinsic oxygen affinity, i.e. a hemoglobin having a P50 of at least about 10 Mm Hg under physiological conditions (e.g. Hb Chico, beta-66 lysine is replaced by threonine; Hb Raleigh, beta-1 valine is replaced by alanine; Hb Titusville, alpha-94 aspartate to asparagine; Hb Beth Israel (beta-102 asparagine is replaced with serine; and Hb Kansas, beta-102 asparagine to threonine; variants that are stable in alkali (e.g. Motown/Hacettepe, beta-127 or glutamine is replaced with glutamic acid); variants that are stable in acid; variants which have a lowered binding affinity to haptoglobin; variants with an increase intrinsic oxygen affinity, i.e. a hemoglobin having a P50 of at most about 1 mm Hg under physiological conditions (e.g. HbA Deer Lodge, beta-2 histidine is replaced with arginine, Labossiere et al., 1972, Clin. Biochem. 5:46-50; HbA Abruzzo, beta-143 histidine is replaced with arginine, Tentori et al., 1972, Clin. Chim. Acta 38:258-262; and HbA McKees Rocks, beta-145 tyrosine is replaced with a termination sequence, Winslow et al., 1976, J. Clin. Invest. 57:772-781).
Acid stable hemoglobin variants may include those that replace the histidine at the alpha-103 position with an amino acid is not ionized in acid (Perutz, 1974, Nature 247:341). Examples of such amino acids include serine, threonine, leucine, and alanine.
Haptoglobin nonbinding hemoglobin variants are those with variation in the alpha-121-127 sequence. This sequence has been shown to be involved in the binding of haptoglobin which is believed incident to increased circulatory clearance (McCormick and Atorssi, 1990, J. Prot. Chem. 9:735).
The globin variants may be produced by various methods known in the art. The manipulations which result in their production can occur at the gene or protein level. The globin may be altered at the gene level by site-specific mutagenesis using well-known procedures. One approach which may be taken involves the use of synthetic oligonucleotides to construct variant globins with base substitutions. In one embodiment, a short oligonucleotide containing the mutation is synthesized and annealed to the single-stranded form of the wild-type globin gene (Zoller and Smith, 1984, DNA 3:479-488). The resulting short heteroduplex can serve as primer for second strand synthesis by DNA polymerase. At the 5' end, a single stranded nick is formed which is closed by DNA ligase. In another embodiment, two complementary oligonucleotides are synthesized, each containing the mutant sequence. The duplex that forms after annealing these complementary oligonucleotides, can be joined to a larger DNA molecule by DNA ligase provided that the ends of both molecules have complementary single-stranded "sticky" ends. Another approach which may be taken involves introducing a small single-stranded gap in the DNA molecule followed by mis-repair DNA synthesis i.e., the misincorporation of a non-complementary nucleotide in the gap (Botstein and Shortle, 1985, Science 229:1193). The incorporation of a thiol nucleotide into the gap may minimize the excision of the non-complementary nucleotide. Alternatively, a globin variant may be prepared by chemically synthesizing the DNA encoding the globin variant using procedures known in the art (see for example Froehler, 1986, Nucl. Acids Res. 14:5399-5407 and Caruthers et al., 1982, GENETIC ENGINEERING, J. K. Setlow and A. Hollaender eds., Plenum Press, New York, vol. 4, pp. 1- 17). In a preferred embodiment, fragments of the variant globin-coding nucleic acid sequence are chemically synthesized and these fragments are subsequently ligated together. The resulting variant globin-coding strands may be amplified using procedures known in the art, e.g. PCR technology, and subsequently inserted into a cloning vector as described in, e.g., PCT Application Publication No. W090/13645, published Nov. 15, 1990). In specific embodiments, site-specific mutants may be created by introducing mismatches into the oligonucleotides used to prime the PCR amplification (Jones and Howard, 1990, Biotechniques 8:178-180).
Manipulations of the globin sequence may be carried out at the protein level. Any of numerous chemical modifications may be carried out by known techniques including but not limited to specific chemical cleavage by cyanogen bromide, trypsin, chymotrypsin, papain, or V8 protease, NaBH4 acetylation, formulation, oxidation, reduction; etc. Alternatively, the variant globin protein may be chemically synthesized using procedures known in the art, such as commercially available peptide synthesizers and the like. Such standard techniques of polypeptide synthesis can be found described in such publications as Merrifield, 1963, J. Chem. Soc. 85:2149-2154 and Hunkapillar et al., 1984, Nature (London) 310:105-111).
The hemoglobin prepared according to the procedures described above may be used for the prophylaxis or treatment of systemic hypotension or septic shock induced by internal nitric oxide production. Such internal nitric oxide production may result from the treatment of an animal with a cytokine which includes but is not limited to tumor necrosis factor, interleukin-1, and interleukin-2, in the case of systemic hypotension. In a specific embodiment, hemoglobin may be used for prophylaxis or treatment of systemic hypotension in a patient induced by chemotherapeutic treatment with such cytokines. In septic shock, the production of nitric oxide results from the exposure of an animal to a bacterial endotoxin. Viral, fungal or enterotoxin-producing bacteria may also be found to directly or indirectly induce NO overproduction alleviatable by administration of the hemoproteins of the present invention.
The preferred iron hemoprotein, hemoglobin, used in the present invention is mixed with a pharmaceutically acceptable carrier. The pharmaceutical carriers are physiologically compatible buffers as Hank's or Ringer's solution, physiological saline, a mixture consisting of saline and glucose, heparinized sodium-citrate-citric acid-dextrose solution, and the like. The hemoglobin for use in the methods of the present invention can be mixed with colloidal-like plasma substitutes and plasma expanders such as linear polysaccharides (e.g. dextran), hydroxyethyl starch, balanced fluid gelatin, and other plasma proteins. Additionally, the hemoglobin may be mixed with water soluble and physiologically acceptable polymeric plasma substitutes, examples of which include polyvinyl alcohol, poly(ethylene oxide), polyvinylpyrrolidone, and ethylene oxide-polypropylene glycol condensates and the like.
Techniques and formulations for administering the compositions comprising iron hemoprotein generally may be found in Remington's Pharmaceutical Sciences, Meade Publishing Col., Easton, Pa., latest edition. In a preferred embodiment, the hemoglobin is administered intravascularly. The amount of iron hemoprotein administered, the effective amount, is an amount sufficient to bind to substantially all of the nitric oxide present, thus removing free nitric oxide from circulation. Such an effective amount is generally in a range of from about 0.1 g/kg body weight to about 10 g/kg body weight.
The following examples are presented to illustrate a best mode and preferred embodiments of the present invention and are not meant to limit the claimed invention unless otherwise specified.
Experiments were carried out on conditioned mongrel dogs weighing 23-28 kg. Animal care was in accordance with the recommendations of the American Association for Accreditation of Laboratory Animal Care and met all standards prescribed by the Guide for the Care and Use of Laboratory Animals (Committee on Care and Use of Laboratory Animals (1978) Guide for the Care and Use of Laboratory Animals (Natl. Inst. Health, Bethesda, Md.) DHEW Publ. No. (NIH) 78-83.
The dog was fasted overnight before the day of the experiment and was anesthetized with pentobarbital (25 mg/kg, i.v.), orotracheally intubated and ventilated with a Harvard pump at a nominal rate of 12 breaths per minute and a tidal volume of 15 ml/kg. The dog was instrumented with a cordis for the introduction of a Swan-ganz catheter for measurement of central venous and pulmonary arterial pressures as well as an arterial line for continuous blood pressure monitoring via a computer driven analog to digital processor.
After the blood pressure and heart rate was stabilized, endotoxin was administered to the dog as a bolus injection (50 μg/kg, i.v. in 10 ml DPBS. After the onset of hypotension, a rapid infusion of 50 ml hemoglobin (5.0 g commercially available bovine hemoglobin/100 ml) over 20 seconds was administered by a central venous catheter. Cardiovascular changes were monitored for an additional 12 hrs. after hemoglobin administration.
A tracing depicting endotoxin-induced hypotension, its inhibition by hemoglobin, and subsequent administration of bovine serum albumin (5 grams) is shown in FIG. 1. Mean arterial blood pressure declined from 160 mm Hg to 70 mm Hg two hours after endotoxin administration. The hemoglobin (HgB) was subsequently administered as an intravenous bolus. The blood pressure drop was abrogated by this administration and the hypotension was partially reversed. This effect lasted approximately 45 minutes before the blood pressure decline resumed. The subsequent decline was only slightly influenced by the administration of 5 grams of bovine serum albumin (BSA). 0n repetition of this experiment, substantially the same results were obtained.
Nitric oxide formation by endothelial cell cytosol was measured by a novel kinetic 96-well microplate assay. The assay is based on the capture of NO by Fe2+ -myoglobin (Mb) which is subsequently oxidized to Fe3+ -myoglobin. The process of heme oxidation was continuously measured in a kinetic microplate reader (Molecular Devices, Menlo Park Calif.) as the rate of change in OD405 -OD650. Data points were collected from all 96 wells every 16 sec. for 30 minutes at 250� C. with shaking prior to each OD measurement. The slope of the best fit regression line (OD/min) was used to calculate the rate of NO-synthesis. OD/min measurements were converted to pmoles NO/min by dividing by the increase in OD405 -OD650 measured upon complete oxidation of 1 pmole of reduced Mb with a 10-fold molar excess of potassium ferricyanide. Under the conditions employed, the efficiency of NO capture by Mb approached 100%; neither doubling nor halving the concentration of Mb influenced the Mb oxidation rate. All samples contained 5-30 μl of endothelial cell cytosol (1-6 mg protein/ml) and final concentrations of 5 μM Mb, 500 μM L-arginine, 500 μM NADPH and 80 Mm TRIS, pH 7.6.
Fe2+ -Myoglobin Preparation: 2 Mm myoglobin (from horse skeletal muscle, sigma) was reduced with a sodium dithionite and immediately applied to a Sephadex G-25 column, followed by elution with 50 Mm TRIS buffer, Ph 7.6. Mb was aliquoted and stored at -700� C. for up to 2 months prior to use. The usefulness of this iron hemoprotein for nitric acid removal is thus clearly shown.
Various references are cited above, the disclosures of which are incorporated in pertinent part by reference herein for the reasons cited.
The invention described and claimed herein is not to be limited in scope by the specific embodiments disclosed, since these embodiments are intended as illustrations of several aspects of the invention. Any equivalent embodiments are intended to be within the scope of this invention. Indeed various modifications of the invention in addition to those shown and described herein will become apparent to those skilled in the art from the foregoing description. Such modifications are also intended to fall within the scope of the appended claims.
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Robertson, S; (2002) Motorcycling and congestion: Definition of behaviours. In: McCabe, PT, (ed.) (Proceedings) Ergonomics Society Annual Conference, Royal Agricultural College, Cirencester, April, 2001. (pp. 273 - 277). Taylor and Francis: London.
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|Proceedings paper
|Motorcycling and congestion: Definition of behaviours
|Ergonomics Society Annual Conference, Royal Agricultural College, Cirencester, April, 2001
|behaviour, CONFERENCE, congestion, driver behaviour, ergonomics, Ergonomics Society, motorcycling, Royal, societies
|UCL classification:
|UCL > School of BEAMS > Faculty of Engineering Science > Civil, Environmental and Geomatic Engineering
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CQB CITY AIRSOFT ACTION September 12th 2010 (Magpul PTS Masada)
Please visit: www.milsimjunkie.com Please follow www.twitter.com ========== Note: Yes, this is a re-upload of the same video which was accidentally set to public in the early hours of Sept 14, 2010. A few of you were able to watch the original video before I was even able to put annotations and links. All you airsoft junkies don't sleep =) Some addressed an issue with the video which was an oversight on my part. I have gone ahead and removed the scene and brought the issue with the party involved. I want to say thanks to those that brought it up and I will continue to make great airsoft videos. Hope you enjoy this re-upload! Thanks for watching! ========== Location: CQB City Website: www.cqbcity.com Date September 12, 2010 ========= Most Notable Weapon of the Day Weapon: Magpul PTS Masada/ACR tinyurl.com ========== Teams/People in the video: BAT (Bay Area Tactical) M4H (Murder 4 Hire) Doom Crew Jon "Ender007″ www.youtube.com
CQB CITY AIRSOFT ACTION NOVEMBER 28th 2010 (MILSIM JUNKIE IS BACK 3 DOG BISCUITS AND A PEPSI)
Please visit: www.milsimjunkie.com Please follow www.twitter.com ========= Special thanks to all those that DONATED in person at CQB CITY or via Paypal. I owe many thanks and gratitude to all of you that continue to watch the videos and support quality CQB action. I am back with a new camera and new stuff to show the airsoft and youtube community. Stay Frosty! ========= Most Notable Weapon of the Day: KWA MAC 11 ========== Location: CQB CITY Stockton, California www.cqbcity.com =========
Video Rating: 4 / 5
Categories: Usa Tags: 2010, 28th, action, Airsoft, Back, BISCUITS, City, JUNKIE, MILSIM, November, Pepsi
AMAZING BASKETBALL ACTION !! Alley-oop !!! Epic !!!
BEST Basketball Play Ever
Ubuntu for Android in action
Canonical introduces 'Ubuntu for Android' Visit here for more details: www.teamphones.com Follow us: twitter.com www.facebook.com plus.google.com
arab SLS FAB Design IN ACTION !!!
⇩⇩⇩⇩⇩⇩ All the info on this video can be found here ⇩⇩⇩⇩⇩⇩ This is the first time you will see this car in action, and where else than in the beautiful streets of Paris! The first real arab supercar I spot in Paris for summer 2011. In this video you will see many driving scenes of this exclusive car (only 5 were made in the world apparently). The first clip is on the Champs Elysées… How awesome is that? This is a white Mercedes SLS AMG "Gullstream" by Fab Design, with plates from Qatar. Such a beautiful car, IMO, although it looks a bit "plastic" in away (hell, it even has a scratch as you can see on my other video: youtu.be ), but awesome nevertheless. I definitely like the wing. This car made its official debut at the 2011 Geneva auto show (which I attended, and have videos of)! ENJOY! ***************************************************************************************** [●] Facebook: www.facebook.com [●] Visit my website: alexsmolik.com [●] FlickR: www.flickr.com [●] Twitter: twitter.com ***************************************************************************************** Hope you enjoy! – ALEX
Airsoft War Systema M4, MAC-11 Airsoft Action サバイバルゲーム
Im on FaceBook At www.facebook.com Main Channel www.youtube.com Alternative Channel www.youtube.com Filmed on Saturday 6th November at the Ai500 Airsoft Event which was held near a town called Leek in Staffordshire, England using a site ran by First and Only Airsoft. If you wish to play here the link for the site is at www.firstandonlyevents.co.uk Airsoft guns can be bought at www.airsoftworld.co.uk For more information on playing Airsoft in the UK and elsewhere I recommend you use the forum on the following website http You will need to set up a login to use the forums, its free.
Video Rating: 4 / 5
4½" NASCAR: Jeff Gordon #24 (Dupont) Motorized Pull Back Action Vehicle 1/43 Scale
4½" NASCAR: Jeff Gordon #24 (Dupont) Motorized Pull Back Action Vehicle 1/43 Scale
- 4½" Nascar Racer 1/43 Scale
- Motorized Pull Back Action
- Authentic Details
- Official Licensed Product by NASCAR
- Not Suitable for Children Under 3 Years Old
4½" Nascar Racer #24 Jeff Gordon, Dupont Car, Motorized Pull Back Action.
Bonus: NASCAR Hologram sweepstakes, Enter Hologram Code at www.NASCAR.COM/Hologram FOR A CHANCE TO WIN.
List Price: $ 9.99
Price: $ 8.00
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Categories: Sports Tags: 1/43, action, Back, Dupont, Gordon, Jeff, Motorized, NASCAR, Pull, Scale, Vehicle
Beyonce a Life in Music (Culture in Action)
Beyonce a Life in Music (Culture in Action)
Introduces children to a range of arts topics. This title offers practical activities to reinforce learning.
List Price: $ 18.12
Price: $ 18.12
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CQB CITY AIRSOFT ACTION DECEMBER 12th 2010 (HERA Arms GCC)
========== Game Day: December 12, 2010 ========== Locaiton: CQB City Stockton, California ========== Most Notable Weapon of the Day: HERA Arms (GCC) Glock Carbine Conversion ========== Scenario: Camo is to find the "down pilot" while Insurgents are to hold their position. Once reclaimed if the down pilot is shot the game is over, Camo is to hold the down pilot until extraction ========== Please Visit: www.milsimjunkie.com Please Follow www.twitter.com
Video Rating: 4 / 5
Albuquerque Action – 2011 Move Beyond Possible Parkour Tour
The first main stop on the tour was in Albuquerque, New Mexico. The land of enchantment surprised us with some amazing architecture on the UNM campus. We were also surprised to see how talented this small, unknown parkour community was. Special thanks to Brandon Douglass who helped us out on short notice and to Levi Meeuwenberg who happened to be in town at the same time and accommodated us! ______________________________________________ Check out www.movebeyondpossible.com for photos and blogs from the tour. ______________________________________________ The 2011 Move Beyond Possible Tour toured the western USA to attend gatherings, run workshops, and document the parkour lifestyle. Photos and videos were produced along the way as the athletes from APEX Movement pushed their limits, explored new places, and met new people. The tour hit up the following locations over 27 days and 5000 miles May 22-24 — Denver, CO May 24-26 — Albuquerque, NM May 26-28 — Phoenix, AZ May 28-29 — Las Vegas, NV May 29-30 — Joshua Tree National Park, CA May 30-June 2 — Los Angeles, CA June 3-6 — San Francisco, CA June 6-8 — Redwood National Park, CA June 8-10 — Portland, OR June 10-12 — Seattle, WA June 12-14 — Vancouver, BC June 15-16 — Bruneau Sand Dunes State Park, ID June 16-18 — Salt Lake City, UT June 18-19 — Arches National Park, UT ______________________________________________ Thanks to our title sponsors: – APEX Movement (www.apexmovement.com) – Movemental (http – Native …
Video Rating: 4 / 5


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Renate DHA Dosage
Generic name: prenatal multivitamin and minerals
Dosage form: tablet
The information at Drugs.com is not a substitute for medical advice. ALWAYS consult your doctor or pharmacist.
women (over 12 years of age), take one (1) caplet and one (1) softgel by mouth daily,
between meals or as prescribed by a physician. Caplet and softgel may be taken
separately. Do not exceed the recommended dosage. Do not administer to children
under 12 years of age or persons age 65 or older. Do not exceed one gram (1,000 mg)
of DHA per day.
More about Renate DHA (multivitamin, prenatal)
Consumer resources
- Other brands: Prenatal Multivitamins, Prenatal Plus, Vitafol Ultra, Vol-Plus, More (275) »


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Hooks feast on the Missions' pitching
Corpus Christi continued its recent rampage on Saturday night, blitzing the San Antonio Missions 8-3 at Whataburger Field.
On the eve of Roger Clemens' tuneup start before a regional television audience, the Hooks (36-25) waltzed to their fifth consecutive victory and opened up a 4.5-game lead over Midland and Frisco in the Texas League's South Division.
Any combination of Hooks wins and Midland or Frisco losses adding up to four will give Corpus Christi the first-half championship and the franchise's first playoff berth.
"The pressure's on (Clemens) now to keep it going," Hooks manager Dave Clark joked.
Clark's charges gave him plenty of reason to be in a joking mood. The Hooks battered San Antonio starter Adam Pettyjohn for seven runs in the first four innings, turning the game into a runaway.
In the Missions' four previous visits to Corpus Christi, they had won at least the first two games of each series. After the Hooks' fast start on Saturday, it was obvious that another one of the many gut-wrenching losses the Missions have put the Hooks through in the past two seasons wasn't in the cards.
A big reason for that was Hooks starter Juan Gutierrez, who continued to dominate on the mound. He struck out eight in 5.2 shutout innings to continue his recent strong run. Gutierrez has allowed only two earned runs in his past four starts, striking out 29 in a span of 26.2 innings.
"I'm feeling really good out there," Gutierrez said. "I've got the location on my fastball where I can throw it for strikes when I want."
Catcher Kevin Davidson said Gutierrez's ability to mix his fastball with his off-speed repertoire has been a big key.
"He's always had a great fastball, but he had trouble finding that changeup, but lately he's had that changeup working for him," Davidson said. "That's helped get the hitters from (keying) on the fastball."
Corpus Christi improved to a league-best 22-10 at home. Fast starts have been the key during the current winning streak. In three of the past four games, the Hooks have had leads of 7-0, 8-2 and 7-0 after four innings.
"It's no secret that we play real well at home," Clark said. "Right now, things are clicking and the pitching is good, and we're swinging the bats and scoring some runs. The big thing is we're scoring those runs early, which allows the starting pitcher to get a little more comfortable out there."
How they scored
Anderson singled to right. Anderson to second on pitcher Pettyjohn's balk.
Zobrist struck out. Pence lined out to left. House singled to left; Anderson scored, House to third on left fielder Bourgeois' fielding error. Bonifay struck out. 1-0 HOOKS
Cortes singled to right. Saccomanno singled to left, Cortes to second. Ash sacrificed to first; Cortes to third, Saccomanno to second. Davidson flied out to center, Cortes scored. Anderson singled to right; Saccomanno scored, Anderson to second on throw to home. Zobrist singled to left; Anderson scored, Zobrist to second on throw to home. Pence fouled out to first. 4-0 HOOKS
House grounded out to third. Bonifay homered to left-center. Cortes grounded out to shortstop. Saccomanno struck out. 5-0 HOOKS
Ash singled to center. Davidson walked, Ash to second. Anderson struck out.
Zobrist singled to left; Ash to third, Davidson to second. Pence hit by pitch; Ash scored, Davidson to third, Zobrist to second. House flied out to center; Davidson scored, Zobrist to third, Pence to second. Bonifay struck out. 7-0 HOOKS
Cortes singled to right. Saccomanno grounded into double play, shortstop to second to first. Ash hit by pitch. Davidson singled to second, Ash to second.
Anderson walked; Ash to third, Davidson to second. Zobrist singled to third; Ash scored, Davidson to third, Anderson to second. Pence grounded into fielder's choice to second, Zobrist out at second.
Castro homered to left. Oliveros grounded out to second. Navarro struck out.
Boucher struck out. 8-1 HOOKS
Bourgeois singled to second. Guzman lined out to shortstop. Arroyo hit for LaHair.
Arroyo reached on fielder's choice to third; Bourgeois out at second, Arroyo to second on second baseman Ash's throwing error. Monzon homered to left, Arroyo and Monzon scored. Balentien flied out to right. 8-3 HOOKS
Contact Greg Rajan at 886-3747 or rajang@ caller.com
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Details and Shopping
Customer Favorite
- FP Exclusive :
- Only sold thru Free People
Soft pointelle knit thigh highs with ribbed trim. Perfect for wearing on their own with a mini or layering over a tight.
*By Free People
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Rated 5 out of 5Â by 2 reviewers.
Rated 5 out of 5Â by akire32 lovely! I love OTK socks for fall and just picked these up in my local FP. I am short with bigger thighs, so while I can pull these to mid-thigh, they don't feel super comfortable there. BUT they don't squeeze me like a sausage either! Which is great! I am 5'2" 112 lbs and these wear comfortably just above my knees. The knit is nice and lightweight and slouch around my ankles, which I like (again, I'm a shorty). The feet are a bit loose though, and I wear a 7.5. I will be wearing these with boots or ankle booties so I find I can layer a no-show ankle sock over the feet to keep them snug. Love them! 8.29.2014
Rated 5 out of 5Â by Natalie_PhotoArt A feeling of soft cashmere Though these socks are acrylic, they have a luxurious cashmere feeling: soft and gentle in texture, subtle in colors. Wear them high or low (slouchy) - all will work. One more good point to these socks is that they are not thick - you don't need to get shoes or sandals one size up to wear them with these socks. 10.6.2014
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Argument leads to woman stabbing her mother, Corpus Christi police say
CORPUS CHRISTI - A woman was arrested Sunday on suspicion of aggravated assault after police say she stabbed her mother.
Mistiphone Rainwater, 41, is accused of stabbing her 59-year-old mother in the chest following an argument, police said.
The stabbing occurred in the 9400 block of Morrow Drive, police said.
Rainwater was being held Monday on $20,000 bail, according to jail officials.
Rainwater's mother was taken to Christus Spohn Hospital Memorial hospital for treatment.
Corpus Christi police Capt. Michael Alanis said because of the investigation he could not comment on the weapon used.
"I will say knife but that is about as far as I can go," he said.
Alanis did not have an update on the mother's condition, he said.
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text: News The Slow Dissolve By Dr. Rebecca Jankovich • Jul 1, 2015 Leaving a relationship is difficult. There is no one "right" way to dissolve a partnership. Power Struggles By Cheryl Erwin • Jun 30, 2015 How to avoid power struggles with your teenager. Artown: Opening Night And Highlights By Danna O'Connor • Jun 30, 2015 tedxreno.com The City of Reno presents the 20th year of Artown this July. The month-long festival kicks off on Wednesday, July 1st at Wingfield Park. Danna O'Connor has a preview with Beth MacMillan, executive director of Artown. KUNR is pleased to again be an Artown Festival Media Sponsor. You can hear updates and event information throughout the month here on Reno Public Radio. New Nevada Law Will Limit Shackling Kids In Court By Michelle Bliss • Jun 26, 2015 A new law passed after the recent legislative session will cut down on the routine shackling of children in Nevada courtrooms. Reno Public Radio's Michelle Bliss reports. Nevada has joined a growing national movement to rethink what's called presumptive shackling of kids in court. Dr. Shawn Marsh is with the National Council of Juvenile and Family Court Judges, based in Reno. He's seen children as young as ten in handcuffs, shackles, and belly chains. Reno Taps Drought Reserves By Michelle Bliss • Jun 24, 2015 The Truckee Meadows Water Authority is now using drought reserves to meet customer demand. Usually, surface water from the Truckee River provides 90 percent of what area customers use, but since river flows are so low, the authority has now tapped into its upstream drought reserves in Boca Reservoir. "We actually anticipated being in this position several weeks ago," says Bill Hauck, senior hydrologist for the agency, "but the rain we had in May brought a significant amount of rainfall which found its way to the river. It basically enhanced our supply by several weeks." UNSOM Health Watch: Trans Voice Clinic By Esther Ciammachilli • Jun 22, 2015 Esther Ciammachilli There's been a lot of talk lately about transgender issues, after the recent transition of Caitlyn Jenner, formerly Bruce Jenner. There are several treatments a person receives while transitioning - such as voice therapy - in order to help them sound more like the gender they are becoming. Today on the University of Nevada School of Medicine Health Watch, we're talking about the Trans Voice and Communication Clinic offered by the Department of Speech Pathology and Audiology at the University of Nevada, Reno. Our guests are Rachael Walden, clinical director for this department and Dr. Veterans Nursing Home Project In Reno Gains Momentum By Michelle Bliss • Jun 19, 2015 There's a need for almost 400 nursing home beds for veterans across the state. That's according to the Department of Veterans Affairs. As that need continues to grow, Reno Public Radio's Michelle Bliss reports that momentum and funding are also growing for the first veterans nursing home in Northern Nevada. Nevada Humanities Festival and Chautauqua 2015 By Danna O'Connor • Jun 19, 2015 The Nevada Humanities Festival and Chautauqua kicks off tonight and runs through the 26th. Details and tickets at www.nevadahumanities.org. The theme of the festival is "The Wilderness." Danna O'Connor chats with Karen Wikander, program manager for Nevada Humanities. Dr. Ben Carson Talked Taxes At UNR By Associated Press • Jun 19, 2015 Carsonscholars.org Republican presidential candidate Dr. Ben Carson stopped in Reno earlier this week to continue his call for major changes to the country's tax code and a more prominent American role in world affairs. "Access" Is Key Word In Veterans' Talk With Hillary Clinton In Reno By Michelle Bliss • Jun 19, 2015 After speaking to Latino leaders in Las Vegas Thursday, Democratic Presidential Candidate Hillary Clinton stopped in Reno to talk about veterans issues. Reno Public Radio's Michelle Bliss reports. Instead of giving a formal speech, Clinton held a roundtable at a VFW in Reno with local panelists who voiced their concerns for veterans in Nevada. "I think that there are two words that should never be in the same sentence," said Democratic State Senator Pat Spearman, "and that's 'homeless veteran.'" Pages1 2 3 4 … next › last »


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Pregnancy Health
Group B Strep & Pregnancy: Who's at Risk
Group B Strep: Here's everything you need to know about the risky bacteria in honor of International GBS Awareness Month this July.Read More
Real Mom Stories
Pregnancy Doesn't Stop Mom & Daughter Dance
Cute alert! Six-year-old Jaylyn and her pregnant mom team up to show off their enviable moves to 'Watch Me' by rapper Silento.Read More
Moms Over 50: Is Sophie B. Hawkins Setting a Trend?
The singer, 50, is pregnant with her second child, while birth rates for older women are on the rise. Here's what doctors say about it.Read More
Health and Development
Could Gazing at Your Newborn Predict ADHD?
New research says your baby's ability to stare might predict her behavior later in life. Here's why.Read More
It's Here! Your 2015 How-to-Be-a-Mom Summer Reading List
Here are readers' top picks for motherhood must-reads. Spoiler alert: No boring textbooks here.Read More
Getting Pregnant
Could Your Fertility Be Written All Over Your Face?
New research shows that facial redness increases around ovulation. But could blushing help couples conceive?Read More
Labor and Delivery
Babies Born via C-Section Benefit from 'Cord Milking'
You've heard of delaying cord clamping, but this newer cord technique is working wonders for preterm babies. Some doctors think full term babies could use it, too. Should you ask for it?Read More
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Surprise! Evangeline Lilly is (Really Quite) Pregnant
The Lost actress astonishes everyone at the L.A. premiere of Ant-Man with an unexpected baby bump. Congrats are in order!Read More
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Veiled Voices | Dr. Su'ad Saleh and Grand Sheikh Tantawi | PBS
Duration: 02:52
Program: Veiled Voices
This is a scene out of the documentary Veiled Voices. It asks the question whether Dr. Su'ad Saleh could be considered for the position of Grant Mufti(ya) in Egypt. The late Grand Sheikh of Al-Azhar Tantawi addresses the question and Dr. Saleh speaks to this as well. Dr. Suad, widely considered the most influential female religious leader in Egypt, leads this fight through her weekly television show, Women's Fatawa, a telephone call-in show in Egypt through which people solicit her advice and religious judgments based in Islamic Sharia Law, fatwas.Tags:
Veiled Voices | Women Leaders in Egypt | PBS
Veiled Voices | Trailer | PBS
Women in Lebanon, Syria and Egypt redefine the role of females as leaders in the Islamic religion. 01:34
Veiled Voices | What is a fatwa? and Who is a...
A brief explanation by Professor Paul Heck of Georgetown University 01:24
Veiled Voices | What is Islamic Law? | PBS
As explained by Professor Paul Heck of Georgetown University 00:51
Veiled Voices | Women as Public Leaders in...
This is an excerpt from our interview with Professor Paul Heck at Georgetown University. He discusses the public... 00:48
Veiled Voices | LADIES OF BRILLIANCE: Students...
Al-Azhar University opened its doors to women over 40 years ago in a separated segregated campus. Students discuss... 08:45
Veiled Voices | Ladies of Brilliance: A...
Al-Azhar University opened its doors to women over 40 years ago in a separated segregated campus. Students discuss... 02:40
Veiled Voices | Outtake: Ghina Hammoud on...
Ghina Hammoud was one of the first veiled women on television in the Arab world. She began broadcasting religious... 01:03
Veiled Voices | Magda's Calling: A Sneak Peek...
Magda Amer is a "daiya" or caller for Islam in Cairo, Egypt. She has is certified by al-Azhar in Egypt to teach... 02:41
Veiled Voices | A Brief History of Women in...
Veiled Voices | Custody Laws in Islam | PBS
Professor Paul Heck of Georgetown University and Sheikh Ziad al-Saheb from Lebanon discuss the complicated issue of... 04:30
Veiled Voices | Socratic Sheikha: Sneak Peek | PBS
Hanan al-Lahham is a religious teacher in Damascus, Syria. Although she did not receive a formal education in... 02:32
Veiled Voices | Mosque of Light: A Sneak Peek...
The Abu Nour School is part of the Sheikh Ahmed Kuftaro Foundation run by the founder's son Sheikh Salah al-Deen... 02:50
Veiled Voices | Outtake: Ghina visits a Student...
Ghina Hammoud makes regular visits to her students who attend her religious lessons at her Al-Ghina Center. She is... 02:59
Veiled Voices | Outtake: Ghina and her Mother...
Ghina's mother recalls one of her proudest moments as mother to Ghina. She discusses the Night of Power during Ramadan 01:28
Veiled Voices | Outtake: Ghina Hammoud's Morning...
This scene was cut from the film but shows a unique glimpse of Ghina Hammoud, a Muslim religious leader in Beirut,... 02:04
Veiled Voices | A Sneak Preview | PBS
Veiled Voices is now finished and get a first glimpse into the opening of the film. 01:49
Wide Angle | Dishing Democracy | PBS (#604)
Full Program: A hit all-female talk show in Cairo features four Arab women who push social reform. 55:07
WIDE ANGLE | Dishing Democracy | Excerpt | PBS (#604)
"Dishing Democracy" goes behind the scenes at Arabic television channel MBC in Cairo and its hit all-female talk show,... 04:15
- Watch more video from this program
Post Date: March 11, 2010


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Straight from our archives, these cold brews taste just as great today.
MyPlate Inspired: 6 Healthy Breakfasts to Start Your Day Right
Treat yourself to down-home goodness without the guilt.
Get the recipe for Double-Corn Spoonbread
Yes, pie for dinner. This mash-up of noodles, ricotta and Parmesan cheeses, and bacon is hard to resist.
Get the recipe for Spaghetti Pie
Dress Up Your Table With DIY Edible Place Cards


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YIELD: Serves 8
In a shallow baking dish whisk together eggs and water and in a large shallow baking dish stir together bread crumbs, zest, thyme, and salt and pepper to taste. Dip cutlets, 1 at a time, into egg mixture, letting excess drip off, and dredge in bread crumb mixture, pressing lightly until mixture adheres. Transfer cutlets as coated to a large rack and let dry at room temperature at least 15 minutes and up to 30 minutes. Veal may be prepared up to this point 1 hour ahead and chilled, uncovered.
Preheat oven to 225°F.
In a deep heavy skillet (preferably cast-iron) heat 1 inch oil over moderately high heat until hot but not smoking. While oil is heating, dredge 4 lemon slices in flour, knocking off excess. Fry lemon slices, turning them occasionally, until golden, about 45 seconds. Transfer slices as fried with tongs to paper towels to drain briefly and arrange in one layer on another large rack set on a baking sheet. Coat and fry remaining 12 lemon slices in batches in same manner. (Carefully remove skillet from heat if necessary while coating slices to prevent oil from overheating.) Keep lemon slices, arranged in one layer, warm on baking sheet in oven.
Remove any food particles in oil remaining in skillet with a slotted spoon and discard. Heat oil if necessary until hot but not smoking and fry cutlets, 1 at a time, turning them once, until golden and just cooked through, about 45 seconds, transferring with tongs to paper towels to drain briefly. Transfer veal to cleaned rack set on another baking sheet and keep warm in oven.


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Thursday, April 22, 2010
© Copyright 2015 Gwinnett Daily Post
DENVER -- It's prime season for tanning beds, when proms and spring beach vacations prompt young women to pack indoor salons in search of bronze skin to go with their new bikinis.
But business is slow this spring. Health warnings about the skin cancer risks of tanning beds, combined with consumers forgoing nonessentials in this recession, have the nation's estimated 18,000 tanning salons on hard times. Now they're bracing for another hit: A 10 percent tax on tanning bed use starts nationwide this July, part of the federal health care overhaul.
''Will I be here next year? I don't know,'' said Kristi Alpers, owner of Cherry Creek Tans in Denver. The tax will add 90 cents to a few dollars for a single tanning session, depending on the machine. It's a fee she thinks will bankrupt her.
''They're trying to ban tanning totally. That's what this is about.''
Congressional tax writers project the tax will raise about $2.7 billion to help expand health coverage to uninsured Americans over the next decade, and they're betting that indoor tanners won't be turned off by a few extra dollars. After all, customers already are paying sales tax on tanning lotions and oils. Why not the tanning service itself?
''I don't care how much it costs, I'm going to do it. I love it,'' said Lisa Mailloux, who tans at Cherry Creek Tans.
The health warnings don't bother 57-year-old Kris Cruzen, a mechanic.
''I've been tanning for 30 years, and look at me,'' Cruzen said, stripping off his shirt before popping on protective eye goggles and climbing into a tanning bed. ''I'm fine.''
But the industry insists it's in danger. Many mom-and-pop salons are going to close, says the Indoor Tanning Association, although the industry group doesn't have figures.
''The jury's still out. We don't know how the public's going to take it,'' said Greg Henson, owner of Heartland Tanning Inc., a Lee's Summit, Mo., tanning bed manufacturer and distributor.
The government says the indoor tanning industry is peddling a known cancer risk.
More like this story
- Young women link tans with attractiveness ( January 3, 2006 )
- States consider banning minors from using tanning beds ( June 27, 2011 )
- CEPEDA: Dark skin won't protect you from sun's deadly rays ( April 2, 2011 )
- Miss Gwinnett County's Outstanding Teen hosts fashion show to benefit charity ( January 26, 2012 )
- Fear not, beach volleyball fans, bikinis aren't going anywhere ( April 9, 2012 )


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Hello all,
I've been thinking about picking up another PRS loaded with soapbar pickups. (I owned a McSoapy Standard for awhile a few years ago. I also owned a Private Stock SCT with P90s.) I have a 1959 Les Paul Junior which sounds amazing, but I really love the tone of a P-90 in the neck as well, which obviously doesn't work too well with the Junior.
I'm kind of torn about which model to get though. I REALLY liked that McCarty Standard I had. But now I'm being bombarded with Korina McSoapies, Custom 22s with 3 P-90s, and Ted McCarty models in Doublecut and Singlecut bodies. Truth be told, I think I'm leaning towards a Ted DC245...And I'd really prefer a Ted with a rosewood neck, but I don't think they made those with P-90s. (Did they?)
Does anyone have any advice or recommendations? Of course, I'd love to try them out, but I can't recall ever seeing a PRS in a store with soapies, except for the Mira models.
Oh yeah, I'd sell body parts for one of those ultra-rare 1999 McCarty models with the Brazilian neck and soapbars.


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As someone who lives in Tel Aviv but writes primarily on American History you could not have said it better. There is much Israel could learn from the US and its' history. That said however wonderful the US is, and generally it is, there is no comparison to living on one's own home
If selected for publication, it will appear as soon as possible on Haaretz.com.


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College soccer
UMBC men roll, 4-0, in America East final
Senior forward Pete Caringi III (Calvert Hall) scored a pair of goals, leading top-seeded and sixth-ranked UMBC to a 4-0 victory over visiting Hartford in the America East championship game Saturday night. The Retrievers (16-1-2), who lead the nation in winning percentage, earned America East's automatic bid to the NCAA tournament for the third time in the past four years. This is the first time UMBC has captured both the league's regular-season and championship titles. UMBC will learn its seeding in the NCAA tournament during the selection show Monday at noon. Caringi scored in the 10th minute, Oumar Ballo (Archbishop Curley) 3:59 into the second half, Caringi again in the 57th minute and Mamadou Kansaye (McDonogh) in the 86th minute. Caringi, Ballo, Kadeem Dacres and Phil Saunders were named to the all-tournament team. Caringi was the tournament Most Valuable Player.


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Incredible Hulk 2008 (2008)
Director: Louis Leterrier Starring: Edward Norton Liv Tyler Tim Roth
Learn more about Incredible Hulk 2008:
Format: DVD
Manufacturer: Universal Home Video
Sku: 208688499
Category Keywords: Action Scientists Superheroes Theatrical Release
See more in Action/Adventure
|Scientist bruce banner desperately hunts for a cure to the gamma radiation that poisoned his cells and unleashes the unbridled force of rage within him: the hulk.
"...a neat thrill ride with an intelligent script by Zak Penn and smart, well-paced direction by the French director of "The Transporter" series... Kirk Honeycutt, The Hollywood Reporter
"Edward Norton's a more evocative actor than Eric Bana, and he supplies all the emotions required by Leterrier and writer Zak Penn. Lawrence Toppman, Charlotte Observer
"...the big green gamma-guy returns to the screen in a purer, more unadulterated, vastly more entertaining form. Marc Savlov, Austin Chronicle
"A super-powered adrenaline blast! Pete Hammond, Hollywood.com
Editor's NoteHailing more from the pulp-melancholic spirit of CBS's 1978-1982 Hulk television series than its 2003 predecessor did, THE INCREDIBLE HULK wordlessly tells the entire gamma ray-afflicted origin story of scientist Bruce Banner's alter-ego within its first three minutes. This credit sequence in particular evokes (albeit with some cinematic weight) the lo-fi, premise-informing quality that one would find at the onset every episode of a cultish TV show, thus making two things quite clear. First, the Edward Norton-starring INCREDIBLE HULK is not a sequel to or a remake of Ang Lee's HULK, but a reboot equipped with an alternate plot, a new dynamic, and a different set of rules. Second, it is director Louis Leterrier's (UNLEASHED, THE TRANSPORTER) express intention to deliver a tightly wound, economically paced thriller in which scenes of drama and action are not mutually exclusive. THE INCREDIBLE HULK replaces cerebral family drama with the story of a lonely, afflicted scientist who, at the start of the movie, is already in hiding, training his mind-body dichotomy. As for the Hulk that inevitably comes forth, a previously poetic weightlessness is rejected in favor of gargantuan physicality, and this time there's an equal behemoth for the not-so-jolly green giant to battle. It's akin to seeing the huge robots of TRANSFORMERS face off, only with more muscle and bone. The angst, of course, isn't gone from this incarnation--not if Norton's intelligently passionate Banner and his tender, forbidden relationship with Dr. Elizabeth Ross (Liv Tyler) have anything to say about it. It's simply that this Hulk always smashes before he ponders.Rounding out THE INCREDIBLE HULK's main players are William Hurt as General Ross and Tim Roth as marine Emil Blonsky, two men in pursuit of the Hulk who, like Banner, aren't so much evil as consumed by tragic character flaws.
Cast & Crew
|Edward Norton
|Tim Blake Nelson
|William Hurt
|Liv Tyler
|Ty Burrell
|Tim Roth
|Gale Anne Hurd - Producer
|Avi Arad - Producer
|Zak Penn - Screenwriter
|Edward Harrison - Screenwriter
|Peter Menzies Jr. - Director of Photography
|Kevin Feige - Producer
|Kurt Williams - Visual Effects Supervisor
|Jim Van Wyck - Executive Producer
|Avi Arad - Executive Producer
|Robert Downey Jr. - Cameo
|Stan Lee - Executive Producer
|Kirk Petruccelli - Production Designer
|Craig Armstrong - Composer
|John Wright - Editor
|David Maisel - Executive Producer
|Louis Leterrier - Director


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2004 R1150RT Wideband O2 Sensor Project (and AF-XIED for BMW)
View Single Post
roger 04 rt
Beastly Adventurer
Joined: Nov 2011
Location: Massachusetts
Oddometer: 2,363
Third Test Ride
Just made a 20 minute local trip at higher RPM. Here's the 911 closed loop info. Tomorrow I plan to take a highway ride cruising between 55 and 75 MPH.
Data Points: 1,400
Driving RPM Range: 3,700 to 5,500 (roughly)
Injectors Fully Off: 3.2% of trip
Open Loop Until Engine Temp: 47 degrees C (115F)
Closed Loop O2:
only 25% of trip
after reaching 47 C
Compared to the Lower RPM run in the previous trip Closed Loop time was much less 25% (higher average RPMs) vs 55% (lower RPMs). Many posters here and other places recommend running the R1150RT at higher RPMs, this has the effect of running a richer average AFR because the motorcycle is Open Loop.
Also, I felt that the bike is "stronger" when it is cold. That feeling makes sense given that it stays Open Loop with richer mixtures until the engine temp reaches (about) 115F. (Wonder if it stays Open Loop if I put a fixed resistor on the Oil Temp Sensor?)
If anyone has an old and/or dead R1150RT O2 Sensor, please PM me. I would like to purchase it for the connector so that I can build a Wide Band replacement system. Thanks.
roger 04 rt
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Plan Your Night: Tonight's fireworks should start at about 10:30 pm: http://t.co/vlUNgxNquC http://t.co/UKYSJpfb0r
The free concert on the Parkway starts now, check out our guide: http://t.co/vlUNgxNquC
Tonight, Sesame Place hosts a dazzling fireworks show at 9:25 pm: http://t.co/WZpLQQs56r
Claim Your Spot! The Top Places To Watch Tonight's Fireworks Above The Art Museum: http://t.co/iC1FJfNwF7 http://t.co/Nnbt0ye566
Long Weekend Picks: Fireworks Above The Art Museum, LGBT 50th Anniversary Celebrations + More: http://t.co/uaU02oeCyw http://t.co/NfLKMTTgRE
Starting at 7 pm, listen to the Roots, Miguel + more perform at the #free 4th of July Jam: http://t.co/vlUNgxNquC http://t.co/UKYSJpfb0r
From 12-7 pm, the Parkway hosts the July 4th Party on the Parkway: http://t.co/vlUNgxNquC
December 22, 2014
Posted by Jillian Wilson
Posted on December 22, 2014 | Jillian Wilson
Filed in:
Attractions, Events, Nightlife
Catching the impressive fireworks display above the Delaware River is an ever-popular way to celebrate the New Year. This year, the display once again returns for two shows — one at 6 p.m. and midnight. Tons of bars and attractions along and just off the waterfront host parties to make it easy for folks to […]
December 19, 2014
Posted by Kristina Jenkins
Posted on December 19, 2014 | Kristina Jenkins
Filed in:
Attractions, Events
Where will you be for the final countdown to the New Year? Ring in 2015 with either of two free fireworks shows on the Delaware River waterfront as SugarHouse Casino once again presents a double dose of New Year's Eve pyrotechnics. For those on the waterfront, listen for piped music in conjunction with the spectacle. […]
November 12, 2014
Posted on November 12, 2014 | Kristina Jenkins
Filed in:
Dining, News
It's time to start prepping the turkey, folks: Thanksgiving Day is less than two weeks away. For those aiming to avoid the cooking, baking, grocery store chaos and post-meal clean up, dozens of restaurants in Philadelphia make it easy to make merry by simply lifting a fork. Beyond sit-down dinners, Philadelphia's dining scene has you […]
July 18, 2014
Posted on July 18, 2014 | Jillian Wilson
Filed in:
Dining, Events, Nightlife
Mark your calendars, Philadelphia! The first-ever Night Out Restaurant Week, organized by SugarHouse Casino, brings lunch and dinner deals to more than a dozen restaurants along Penn's Landing, in Fishtown and Northern Liberties. Depending on the restaurant, fill up on $10 or $20 lunches and $25 or $35 dinners. Visit Bar Ferdinand for a $35 […]
June 9, 2014
Posted on June 9, 2014 | Jillian Wilson
Filed in:
Attractions, Dining, Events, Weekend
It's time to show pop some love. Father's Day is this Sunday, June 15, and to celebrate dear ol' dad, a slew of Philadelphia restaurants, bars and attractions host Father's Day celebrations all weekend long. From free steak dinners and free beer for Dad, to an outdoor extravaganza on Independence Mall, making every father in […]
May 19, 2014
Posted on May 19, 2014 | Jillian Wilson
Filed in:
Events, Weekend
Memorial Day weekend hits this Friday, May 23, and we can't help but feel pumped for the unofficial start of summer and the arrival of warm-weather good times. This season has already been filled with awesome events, and the coming Memorial Day weekend continues the springtime trend of fun-filled Saturdays and Sundays in Philadelphia. The […]
April 10, 2014
Posted on April 10, 2014 | Jillian Wilson
Filed in:
Dining, Events
The Easter holiday, which falls on Sunday, April 20 this year, is quickly approaching, bringing with it a number of festive brunches, decadent dinners and family-friendly egg hunts to the City of Brotherly Love. For youngsters, South Street hosts its annual Easter Promenade complete with a parade, costume contests and a visit for from the […]
December 20, 2013
Posted on December 20, 2013 | Kristina Jenkins
The last days of 2013 are ticking by, Philadelphia! Where will you be for the final countdown? Ring in 2014 with either of two free fireworks shows on the Delaware River Waterfront as SugarHouse Casino once again presents a double dose of New Year's pyrotechnics. The first show, at 6 p.m., is titled "A Salute […]
December 19, 2013
Posted on December 19, 2013 | Jillian Wilson
Filed in:
Events, Nightlife
This year, New Year's Eve in Philadelphia will once again include two fireworks shows at both 6 p.m. and midnight as SugarHouse Casino presents New Year's Eve fireworks on the Delaware River Waterfront. Whether you're looking to catch the 6 p.m. fireworks or the midnight fireworks, numerous parties that line the waterfront, in both Philadelphia […]
December 11, 2013
Posted on December 11, 2013 | Jillian Wilson
[This holiday season, let Uwishunu be your merry guide. From brilliant lights and must-see attractions to festive events and jolly good times, check out our daily updates on holiday happenings in Philadelphia right here.] The holiday season is full of gift wrapping, cocktail parties, cookie swaps, tree cutting and nights of home decorating. Save some […]


text:
Enlarge Cover
Random House | Dec 13, 2005
| 208 Pages
Random House
| Dec 13, 2005
| 208 Pages
This book will introduce you to yourself.Despite what you may have been told since you were a child, you cannot be whatever you want to be in life–you are already what you were meant to be. The secret to uncovering who you are, and your purpose, is built into you in the form of a code–the identity code. Much like your genetic code, your identity code provides a complete map of how you were designed to live.Answer the questions that frame the identity code, and the contours of your life will shift. You will not only emerge stronger, you will emerge larger. Larger in heart, larger in influence, larger in your capacity to love and be loved. You will understand the why of your life.In this life-transforming book, Larry Ackerman shows you how to crack your identity code. With more than twenty years of experience helping organizations and individuals identify their purpose, Ackerman reveals the Laws of Identity and the Eight Essential Questions they contain. As you answer these questions, your identity will gradually become clear. It will become the foundation from which you'll make truly meaningful decisions about what work is right for you, how to build and maintain relationships that matter, and even what interests and hobbies make sense for you. These eight questions, and the call to action each one implies, areWHO AM I?: Define yourself as separate from all othersWHAT MAKES ME SPECIAL?: Unearth what you loveIS THERE A PATTERN TO MY LIFE?: Make the connections that explain past events and foreshadow your futureWHERE AM I GOING?: Use what you've learned so far to guide you on your pathWHAT IS MY GIFT?: Follow the signs of joyWHO CAN I TRUST?: Take stock of who matters–and whyWHAT IS MY MESSAGE?: Declare yourself on the strength of your giftWILL MY LIFE BE RICH?: Surrender to the pull of your identityAs Ackerman points out, unbridled freedom actually weighs you down. The myth of personal freedom–the notion that you have infinite choices in the course you set for yourself–is the unspoken agony of the modern person. True freedom comes with knowing your identity: the unique characteristics that define your potential for creating value in the world, for making a contribution that springs naturally from the core of your being and touches the lives of others. Within this framework, life's seeming boundaries melt away. Intelligent, provocative, and always practical, The Identity Code sets the reader on the classic quest: the discovery of self. Take the journey.From the Hardcover edition.
Larry Ackerman is a leading authority on organizational and personal identity. As group director for the international identity- and brand-consulting firm Siegel & Gale, Ackerman is widely regarded as the pioneer in the field of identity-based management. His many and… More about Laurence Ackerman
Visit other sites on the Penguin Random House Network


text:
Texas Tech Business Students Return to New Building
"Rawls 2.0" Opens Doors to Green Architecture, Hi-Tech Infrastructure
PR Newswire
LUBBOCK, Texas, Jan. 19, 2012
LUBBOCK, Texas, Jan. 19, 2012 /PRNewswire/ -- Texas Tech University Rawls College of Business students return this week to a "greener" side of the fence as they enter a new building -- focused not on the landscaping, but on the environmentally acclaimed architecture of the new facility.
The nearly 150-thousand-square-foot facility, designed by Boston-based architectural firm Goody Clancy, was built for LEED-certification by the U.S. Green Building Council. LEED (Leadership in Energy and Environmental Design) certification requires buildings to achieve high performance in key areas of human and environmental health, including sustainable site development, water savings, energy efficiency, materials selection and indoor environmental quality.
"It was important to us that we build this new facility with all facets of a 'green environment' in mind," said Debbie Laverie, Ph.D., Rawls College of Business senior associate dean. "We've been a leader in our nation in a number of areas as a College, and we need to continue that momentum even in the very bricks and mortar of this new building for the next generation of leaders we turn out. Garnering official certification from LEED (expected by June) will be yet another honor for us."
In addition to four detention ponds to capture storm water for reuse on site, the new College's structure is built with nearly 75 percent recycled materials -- much of it derived from the demolition of two former on-campus residence halls. Inside the inviting facility, even the colorful broken-glass detail throughout the interior of the building's walls is recycled.
Utilizing West Texas' many days of sun, natural light is plentiful in the building. And, according to Rawls professor of accounting Don Clancy, this is good for both the environment and students' moods. Clancy has been a part of the University's overall building planning for more than 11 years.
On the flip side, exterior glass used throughout the building blocks 40 percent of the sun, he explained, and some of the windows block 60 percent. This stops the heat or cold from getting inside the building, therefore requiring less electricity for heating or cooling.
As for the high-tech infrastructure, a movie-theater-style auditorium features a 10-by-16-foot screen used for teaching, boasting a resolution higher than high definition. In tiered classrooms, each seat is wired for power and Internet, while flat classrooms have wireless Internet. Additionally, five print kiosks located throughout the building allow students and faculty to swipe ID cards and print directly to printers.
"We're also excited about the computer screens located outside each classroom door, where class schedules or emergency notifications can be posted at a moment's notice," Laverie said. "It's going to be an exciting semester -- and this is just the start of a new generation for Rawls."
For more information about the Rawls College of Business, please visit http://www.ba.ttu.edu. For more information about the U.S. Green Building Council, please visit http://www.usgbc.org.
CONTACT: Kim Davis, Nomiss Communication
(806) 544-4255
This press release was issued through eReleases(R). For more information, visit eReleases Press Release Distribution at http://www.ereleases.com.
SOURCE Texas Tech University Rawls College of Business
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text:
BeneFit Cosmetics They're Real
2037 reviews
52% would repurchase
Package Quality: 3.9
Price: $$$
Package Quality: 3.9
Price: $$$
INGREDIENTSWater (Aqua), Paraffin, Polybutene, Styrene/Acrylates/Ammonium Methacrylate Copolymer, Beeswax (Cera Alba), Bis-Diglyceryl Polyacyladipate-2, C18-36 Acid Triglyceride, Palmitic Acid, Stearic Acid, Triethanolamine, Vp/Eicosene Copolymer, Acacia Senegal Gum, Hydroxyethylcellulose, Phenoxyethanol, Tetrasodium Edta, Butylene Glycol, Caprylyl Glycol, Tocopheryl Acetate, Sodium Laureth-12 Sulfate, Glycerin, Potassium Sorbate, Sericin, Calcium Chloride, Sodium Hyaluronate, Tilia Tomentosa Bud Extract, Citric Acid, Bht, Sorbic Acid. [+/-: Iron Oxides (Ci 77491, Ci 77492, Ci 77499), Ultramarines (Ci 77007), Chromium Oxide Greens (Ci 77288), Chromium Hydroxide Green (Ci 77289), Titanium Dioxide (Ci 77891)]. N° 05827/N
on 1/28/2015 10:47:00 AM
Age: 25-29
Skin: Oily, Medium, Not Sure
Hair: Brunette, Other, Other
Eyes: Brown
Definitely NOT my favorite and NOT worth the price.
on 1/28/2015 5:36:00 AM
Age: 44-55
Skin: Sensitive, Fair-Medium, Not Sure
Hair: Brunette, Other, Other
Eyes: Hazel
The brush is nice. The product is in my opinion overrated. The product did not perform for me. Cheap promises! The mascara does not hold the curl and no 'oomph' effect. A no go for me.
on 1/26/2015 10:56:00 AM
Age: 19-24
Skin: Oily, Fair-Medium, Not Sure
Hair: Brown, Other, Other
Eyes: Hazel
I really wanted to like this. I bought this last year and when I first put it on I really loved it. Made my eyelashes long and full but after using it a few weeks I had problems... Problem number one is taking it off. What a MESS you absolutely need a good makeup remover to get this off. Every time I shower or water hits my face it immediately runs all down my face and I have to literally scrub my eyes with makeup remover to get this off. I also found that it dries out on your lashes really quickly which makes it hard to apply multiple coats. There are better mascara's out there. Don't fall for this one!
on 1/25/2015 3:01:00 PM
Age: 25-29
Skin: Normal, Fair-Medium, Not Sure
Hair: Brown, Straight, Medium
Eyes: Brown
I received a sample of this from sephora and it has got to be one of the worst mascaras I have ever used. I usually curl my lashes after applying mascara because I find that it holds a better curl. This mascara practically glued my lashes to the curler and I had a lot of difficulty freeing my poor lashes. Despite my efforts the curl didn't last an hour. Next I tried to curl them first and then apply mascara but that didn't work either. mascara is clumpy beyond belief
on 1/24/2015 10:42:00 PM
Age: 25-29
Skin: Combination, Fair-Medium, Warm
Hair: Black, Wavy, Medium
Eyes: Brown
I switch off between this and DiorShow mascara, but lately I've been buying this more because I can't find DiorShow in a travel size year round. I've only noticed it available during the holiday months. I try to buy the travel sized versions of mascara because although I use it every day, I've noticed that normal sized mascara (of any brand) would go bad and start to smell funky before my usual three month disposal. They're Real travel size sells at Sephora for about $10 (USD). My only requirement of mascara is that it gives me volume. I'm Asian, and if you can't see my eyelashes in pictures with the help of mascara, then I don't buy it again. Thickness doesn't matter as much to me, but with They're Real I get both! I love it. Please note, I don't think that it lengthens, but again, that's not a priority for me. Definitely a HG product for me.
on 1/24/2015 7:39:00 PM
Age: 18 & Under
Skin: Normal, Olive, Warm
Hair: Black, Other, Other
Eyes: Brown
I use this mascara a lot & I do really like it. My eyelashes are very thick but short :( so I use this one on both bottom & top lashes but with the MUFE mascara to give them more length. It's not the BEST mascara I've tried but definitely nessacary to have, it does give a significant amount of length, but not as much as I like, because I like the false lash look. But definitely great for a daily wear.
on 1/23/2015 8:08:00 PM
Age: 36-43
Skin: Oily, Fair-Medium, Not Sure
Hair: Red, Other, Other
Eyes: Blue
One of the worse mascaras I've used. It did nothing for my lashes at all.
It didn't irritate my eyes so that's good. Doesn't rub off or give me racoon eyes. The brush is neither here nor there.
This is one of those mascaras people clearly either love or dislike. I'd try to get a sample of this to try before purchasing this. But, thankfully Sephora has a great return policy so we can all try and find what works best for us!
on 1/23/2015 3:58:00 PM
Age: 18 & Under
Skin: Dry, Fair, Not Sure
Hair: Brunette, Other, Other
Eyes: Blue
I love love love this mascara! If you have the money then it's worth it. It separates and looks gorgeous. It's my favourite mascara!
on 1/22/2015 6:13:00 AM
Age: 18 & Under
Skin: Combination, Medium, Not Sure
Hair: Black, Straight, Other
Eyes: Brown
Not worth it. Nice brush, but the formula is WAYY too runny (and somehow thick at the same time) for my asian lashes.
on 1/21/2015 10:52:00 PM
Age: Unknown
Skin: Normal, Fair, Cool
Hair: Blond, Wavy, Coarse
Eyes: Green
Possibly the worst mascara I have ever tried.
With only one coat, this stuff would not dry. My lashes felt ( and looked) like they were wet with glue, were heavy and drooped. I could even feel dust and dirt stick to them everytime the wind kicked up.
Returned for a refund.
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text:
Updated 02/25/2014
Comfortably hip is the name of the game at this Brooklyn hangout. Grab a beer in the "library" area up front, cozy up around the fireplace in winter, or if you're up for a little sport, join the friendly locals in a game of bocce ball. In the basement, check out some rising indie rock stars on stage.
702 Union St., at 5th Ave., Brooklyn, New York, 11215, United States
Website: www.unionhallny.com
Updated 02/25/2014
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The post-void residual (PVR) urine test measures the amount of urine
left in the bladder after urination. The amount can be measured by draining the
bladder with a thin flexible tube (catheter) or by using ultrasound. The
catheter method has a slight risk of causing infection or injury to the tube
leading from the bladder (urethra). However, the catheter method is safe when
done carefully, and it may be less expensive than ultrasound.
It is not clear how the results of the PVR test can be used to
predict outcomes in men with
benign prostatic hyperplasia (BPH).
Last Revised:
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Job Description
Are you looking for a full time career in social media? NetMediaGroup is seeking a creatively inspired Social Media Manager who can help entice comments, likes, shares and user-generated content on our clients social media content on networks like Facebook, Twitter, Pinterest, and more. You will be involved not only in the creation, monitoring and reporting of all social media but also the strategy. We???re looking for someone who has not only done this for themselves, but also for real life businesses. NetMediaGroup is a full service online marketing agency located in Salt Lake City, Utah. We are seeking an energetic and creative new team member who has experience in social media but is willing to learn. We need someone who is dependable and great at problem solving. This position requires a savvy and confident employee that understands the value of teamwork, continuous innovation with solid communication/presentation skills. Our ideal candidate has business experience with Social Media platforms like: ??? Facebook ??? Twitter ??? Google+ ??? Instagram ??? Pinterest ??? YouTube ??? LinkedIn ??? WordPress Overview of Responsibilities ??? Create, monitor and report on all social media campaigns ??? Monitor all social media outlets: pages, sites, blogs, etc. on daily basis and post and/or respond to fans and followers. ??? Develop monthly calendar for online marketing activities. ??? Research and recommend new marketing tools, sites, apps as well as related content. ??? Maintain client and partner relationships. ??? Coordinate with internal creative and technical teams to produce digital assets. ??? Keep current on new technology, market changes, competition and legal concerns.
Relocation costs may be covered.
??? Social media experience working with businesses ??? 1+ year social media experience. ??? Effective verbal and writing skills. ??? BA/BS degree in Marketing or requisite experience. ??? Ability to meet deadlines within specified time constraints. ??? Has initiative and is proactive. ??? Proficiency in Microsoft WORD and EXCEL. ??? HTML/CSS knowledge (Preferred but not required). ??? Strong organizational, project management, and time management skills a must. ??? Must be good at multi-tasking.
Additional Information
Competitive salary and full benefits. Please email a copy of your resume, along with a brief write up of why you can rock this career in social media, to firstname.lastname@example.com.
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text:
In ancient times, when Britain's trade was arms,
And the loved music of her youth, alarms;
A god-like race sustained fair England's fame:
Who has not heard of gallant Percy's name?
In rival Rome and Carthage never rose!
Ay, and of Douglas? Such illustrious foes 5
From age to age bright shone the British fire,
And every hero was a hero's sire.
When powerful fate decreed one warrior's doom,
But whilst these generous rivals fought and fell, These generous rivals loved each other well:
Up sprung the phœnix from his parent's tomb. 10
Though many a bloody field was lost and won,
Nothing in hate, in honor all was done.
Fast came the Douglas, with his Scottish spears; And, when proud Douglas made his king his foe,
When Percy, wronged, defied his prince or peers, 15
For Douglas, Percy bent his English bow.
Expelled their native homes by adverse fate,
Then blazed the castle, at the midnight hour, For him whose arms had shook its firmest tow'r.
They knocked alternate at each other's gate: 20
This night a Douglas your protection claims;
A wife! a mother! Pity's softest names:
And grant your suppliant all she begs, a tear. In confidence she begs; and hopes to find
The story of her woes indulgent hear, 25
Each English breast, like noble Percy's, kind.


text:
240 reviews
By installing you agree to the Terms of Use and other terms
PublisherLife Before Us LLC
Download size1 MB
Last updated3/7/2015
Works with
- Windows Phone 8.1
- Windows Phone 8
App requires
- contacts
- phone identity
- owner identity
- video and still capture
- location services
- maps
- music library
- photos library
- media playback
- data services
- push notification service
- web browser component
- HD720P (720x1280)
- WVGA (480x800)
- WXGA (768x1280)
Supported languages (1)
English (United States)
The simplest & most efficient communication tool in the world. Yo is a single-tap zero character communication tool. Yo is everything and anything, it all depends on you, the recipient and the time of the Yo. Wanna say "good morning"? just Yo. Wanna say "Baby I'm thinking about you"? - Yo. "I've finished my meeting, come by my office" - Yo. "Are you up?" - Yo. The possibilities are endless. We don't want your email, Facebook, there is no search, no nothing. just Yo. Open the app, tap Yo, that's it. It's that simple. Yo. Aside from communicating with your friends and family you can also get Yo's from what ever you like and interest you. The Yo Index - see what Yo's you can subscribe to as provided by different websites and services. subscribe to what interest you and get notified on it. If you see a star next to the Yo, tap it to see the attachment. Yo your location with double tap! 188.8.131.52 - Yo Store: Subscribe to top accounts like BuzzFeed, the NBA and USA TODAY from the Yo Store. - Bug Fixes - Improvements If you like Yo please take a minute to review us Yo!
4/17/2015Strange but interesting
3/16/2015Would be perfect if we could review yo's and send links in yo's.
3/8/2015Great work.... Thanks for the new update
3/7/2015Add functionality from iOS and Android ;)
3/5/2015The most pointless, time wasting app, yo.
3/1/2015Update the how to, for example it doesn't show how to send your location although it works (double tap). While in the app, notifications are barely readable because of the transparent background... update: uses tons of battery when browsing extensions, otherwise seems fine.
2/20/2015It's cool
2/9/2015Pretty fun app. Kind of dumb but depending what you use it for it can be cool. I wish I could send links to friends though
2/7/2015Cool app


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Go to http://www.3dongle4free.co.uk and create an "account". It will email you your code you need to add to get a ZTE USB dongle + 3 PrePay SIM FREE. Then all you do is pay the postage - £4.95!
I'm going to see if I can unlock mine, but even so under £5 for a USB dongle as a backup is a very good price. With the Mobile Broadband prepay deal this will get you 1gb of data for £10 to use within 30 days. Not brilliant, but if you get stuck it's a good idea. Even better if you have a data deal with 3 already, just pop your SIM in!
[Edit by Paul: I ordered one too and got it next day! Don't forget you can get 1 month unlimited internet for £5 by clicking this link (you must be logged into 'my 3' first.]