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Resolution: standard / high
Figure 6.
Sustained HMGA1a-eGFP expression interferes with the expression of myogenic genes. (A) Comparison of expression profiles of myogenic transcription factors in myoblasts (day 0) and during differentiation (days 1, 3, 6, 9 after induction). Gene expression was analyzed by RT-PCR as described in Fig. 1. Genes analyzed were: myogenic factors 5 and 6 (Myf5 and Myf6), the myocyte enhancer factor 2A (Mef2a), myogenic determination gene 1 (MyoD), myogenin and homeobox, msh-like 1 (Msx1). Note the specific down-regulation of MyoD and myogenin and the up-regulation of the myogenic inhibitor Msx1 in C2A1a cells. As loading control Gapdh expression is shown. (B) Expression profiles of components of the Igf-system in C2C12 and C2A1a cells at the time points described in (A). Genes analyzed were: insulin-like growth factors 1 and 2 (Igf1 and Igf2) and Igf binding proteins 1, 2 and 3 (Igfbp1, Igfbp2 and Igfbp3). Note the suppression of Igf1, Igf2, Igfbp2 and Igfbp3. Gapdh expression is presented as a control.
Brocher et al. BMC Cell Biology 2010 11:64 doi:10.1186/1471-2121-11-64


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Why are guys so complicated?
I mean. I know they say that girls are the complicated gender, but honestly.. guys never show their feelings and it makes us really self conscious!... Show More
Most Helpful Opinion
"We never know how they feel and they keep giving us mixed signals.. "
Trust what your gut feelings (the "I just know" kind of feeling) are telling you. They never lie to you, nor can they be lied to. That's the best way to know. If you have to rely on what others tell you, through words and other things like body languages, you are putting yourself at risk, how high that risk is depends on who you are communicating with. This is the same for both sexes.
As for what guys want, see if these links help?
What are the major turn-offs for guys? link
What personalities do guys look for in girls? link
How can I be a better girlfriend? link
What Guys Said 7
If we put ourselves out there, we might get hurt. =(
I'm pretty expressive, and I let people know what's on my mind. Maybe that's why I never saw guys as very complicated. Oh right. Because I am a guy...
With guys it's usually black and white. There's no tricks, no walk around, no shortcut. When guys say something, they usually mean it. Analyzing is not good! It really is straight forward unless it's one of those "shifty" types. >.>
I'm really not sure what I can say besides that, but that's what I've found. I'm one of those sensitive types, so I let my emotions show. I have a hard time relating to other men.
Good luck. Maybe there's a sensitive guy coming to a theater near you!
Yup, that could be the case all right. I'm neither good at giving signals nor noticing them that's why I think girls are so complicated :P since I don't get what the heck they want. I prefer if girls just say what they feel as opposed to giving signals through body language. That way nothing can go wrong even if the guy is in denial of his own feelings. So just talk to the guy and ask him how he feels; the result would be way more the way I see it.
As an older man I can share my views on this topic with out hesitation. Many people go into a relationship with a set of ideas, rules and a form of a reality that they think is reasonable. However, if you only scratch the surface of Love to a committed relationship you will be disappointed. There is an author Erick Fromm who wrote an article The Art Of Loving. The overall concept of this articles says you must place a relationship as priority as you do your work, your family... You cannot half commit and expect wonderful results. Communication and liberating yourself and your partner is the true epitome of living for love...
We don't want to show our feelings, we want other people to see us as common people, as strong real men. But I tell you, we have strong feelings and we sometimes cry when we are alone or when we are with a person very close to us. If a guy strives on love, he will do everything for it. If the guy strives for his religion, he will do everything to serve their god. If the guy strives for success, he will do what he can for it. If he seeks close friendship, he will strive for it wholeheartedly, and etc. Whatever he strives into, he wholeheartedly do what he can for it. However, he greatly hides his feelings and doesn't want people to see those. The same also to women, I think.
What Girls Said 3
I learned something pretty valuable..guys are not complicated..we make them complicated.
If a guy is quiet, its easy to freak out..wondering what he's thinking of, how it affects us, what it means to us that he doesn't want to talk. In truth, he probably simply has nothing to say.
A guy can say something...and its easy to read between the lines...interpreting and analyzing his tone, his expression and get a much deeper meaning out of it...when in truth..he said what he had to say..nothing less nothing more.
Im not saying guys are transparent...far from it. But generally speaking...we can tear ourselves up over nothing save for what we've created in our heads.. In truth...the less we perceive, the more we see... that applies to understanding how a guy feels as well. Don't read between the lines or expect long heartfelt "share our feelngs" sessions... just pay attention to how he behaves.
As a female, I am struggling with this question myself. I had what felt like a great relationship, and a really compassionate guy. He never failed to express how he felt for me. We had a deep connection from the beginning and a one year relationship. But then things seemed to take a 180. I'm not even sure what happened. Yea we had outside things to deal with causing stress, but this was nothing knew and we usually could always communicate our way through it. But then less and less he wanted to talk. The problem of him not having a job caused us to not be able to go out as much and stress. One day he broke up with me, I'm sure it wasn't because of another girl. In the months following we still had contact and he expressed his love for me on different occasions, but we never officially got back together. We still had that special connection though. But less and less he wanted to talk, he did get a job early on, but I don't know if its commitment issues, when he was so committed at first for a year, or what. I don't know what is going on in his head, and he may be talking to someone else, love? idk. I never ever cheated or anything like that, but am struggling with how our relationship quickly turned so sour, please help!
But what if they really don't show any real signs, you see sometimes a mom can have those gut feelings thinking " oh well he is alright" or just okay.
But for the crushing girl thats different... Attitude and or body language are signs in saying they either have a positive, netrual or negative type of language they either want to share or just won't.
Guys have just to me changed so much, its not like the moives ((never saying that it was just saying)) guys won't go up to you or poke you and say hi.
Guys just seem to want the girl not only to say hi.. They always want them to do all the talking, bieng that conversation starter and its really annoying for me.
I don't want to trust my gut most times on how a guy is feeling, i don't want to be that conversation starter. i don't always have anything to say or talk about so why can't they?
Relationship is built on 50/50 effort..
If he is only going 30 or 20 keeping to himself then my question is why.
You can't do that in a relationship, you just can't because for the girl they choose to be with, its hard and annoying always trying to communicate to a lost cause.
Sorry if i offended someone, its just REALLY annoying when you say you WANT a girlfriend but you choose not to trust her or have her always having to keep pestering about what to say if they don't even try to talk to you.
Have an opinion?


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Originally written on Tireball.com | Last updated 2/5/13
Right handed pitcher Chris Carpenter may be headed for retirement. Carpenter experienced numbness and bruising in his right shoulder and hard after throwing off a mound. While Carpenter has not announced that he plans to retire the Cardinals and general manager John Mozeliak will have to fill the void of not having him in the clubhouse or on the mound. Carpenter missed nearly all of the 2012 season following surgery for thoracic outlet syndrome but had reported that he was feeling good a month ago. The Cardinals are not expected to renew their interest in Kyle Lohse who pitched for the team the last four seasons.
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Celebrity Central / Top 25 Celebs
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Emma Stone & Andrew Garfield Are Back Together!
The actors are spotted around L.A. six weeks after it was revealed they were taking a break
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The actress tells PEOPLE she's spent some time swiping on the app "for a friend"
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The star opens up in a May cover story for Interview with Diane Keaton
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The action hero tackles Swift's "Shake It Off" in a teaser for the new Spike TV series Lip Sync Battle
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Guardians of the Galaxy, The Fault in Our Stars and Neighbors scored top noms for the awards show, which will air April 12


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Going up to the sky after 26 months of storage in Malta.
Finally he's in the air were it belongs. 'TAM 9395' After 2 years and 2 months at Malta on storage, he's back outbound to Rio De Janeiro.. Hope to see you again :)
TAM9395 on her way back to Rio after 25 months of storage in LMML. Landed in Malta on the 20th of October 2011.
Climbing away from her home base of almost 10 years one last time as she returns to France as SQ8894. Delivered new to Singapore Airlines on January 23, 2004.
First visit to LHR and she must have been pleased as a lots of spotters turned out to greet her.


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30 year old woman | Westwood, CA, USA | Seeking men 26-41 within 50 miles of Los Angeles, CA
Active within 24 hours
I'm the kind of girl you've been dreaming of...
I'm silly and love to laugh. I have a sweet tooth that won't quit. I love to cuddle. I'm romantic. I'm a girly girl, that loves the outdoors (yes, I will go camping with you). I enjoy getting away, the more spontaneous the better! I like karaoke. If you challenge me, i'm quite competitive. I give amazing massages. I would eat breakfast for every single meal if I could. I make amazing bread pudding. My ideal guy will get me inside out. Be a great person, with integrity. Be willing to go on adventures with me. Will charm me with just a smile.
Camping , Coffee and conversation , Business networking , Cooking , Dining out , Movies/Videos , Museums and art , Music and concerts , Exploring new areas , Nightclubs/Dancing , Performing arts , Playing sports , Shopping/Antiques , Travel/Sightseeing , Volunteering , Wine tasting
Basketball, Billiards / Pool, Bowling, Dancing, Other types of exercise, Tennis / Racquet sports, Walking / Hiking, Weights / Machines
I exercise 3-4 times per week.
I have Cats
I have an adorable gray and white kitty named Skittles, like the candy.
Middle of the Road
My favorite places are Nobu, Boa Steakhouse, Sky-Bar,and Sprinkles for dessert.. Destinations: Scottsdale, Maui, New York, Santa Barbara, Laguna Beach.
California State University, Northridge, Northridge, CA
Wine & Cheese Days at the Beach Sushi Boardwalk Empire Malibu Dance music Sleeping in Breakfast
Beach days, farmer's markets, sushi, picnics, tennis, hiking, movies, camping, wine tasting, bowling, cooking, weekend getaways.
Girl with a Dragon Tattoo
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What she is looking for
|5'8" (172cms)
|5'9" (175cms) to 6'4" (193cms)
|Body type:
|Slender, About average, Athletic and toned
|Blue, Brown, Green, Hazel
|Light brown, Dark brown, Blonde, Dark blonde
|No Way
|No Way, Occasionally
|Social Drinker
|Social Drinker, Regularly, Moderately
|Other profession
|Artistic / Creative / Performance, Executive / Management, Financial / Accounting / Real Estate, Legal, Medical / Dental / Veterinary / Fitness, Political / Govt / Civil Service / Military, Sales / Marketing, Self-Employed / Entrepreneur, Travel / Hospitality / Transportation, Law enforcement / Security / Military, Fashion / Model / Beauty, Architecture / Interior design
I work as a personal assistant for a well known entertainment company. There is never a dull moment!
|I'll tell you later
|No preference
|Never Married
|Never Married, Widow / Widower, Divorced
|Have kids:
|Yes, and they sometimes live at home, No, Yes, and they live away from home
|Wants kids:
|Definitely, Someday
|White / Caucasian
|Asian, Latino / Hispanic, White / Caucasian
|Agnostic, Atheist, Christian / Catholic, Christian / Protestant, Jewish, Spiritual but not religious, Christian / Other
|English, Spanish
|Bachelors degree
|Bachelors degree, Graduate degree, PhD / Post Doctoral


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