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One of the major obstacles to the exploitation of genetic variation in human medicine, veterinary medicine, and animal breeding is the difficulty in defining haplotypes in unrelated individuals.
We have developed a Multiplex Double Amplification Refractory Mutation System combined with Solid Phase PCR on Fluorescently labelled beads. The process is inherently amenable to automation. It provides a high degree of internal Quality Control, as each PCR product is represented in duplicate on the bead array, and each SNP is tested against multiple partners. This technique can resolve very complex genotypes into their constituent haplotypes; it defined all the alleles at 60 SNP in exon 2 of the ovine DRB1 MHC locus in a sample of 109 rams. These 60 SNP formed 33 DRB1 exon 2 alleles; two of which had not been previously identified; although both of them have been independently confirmed.
This technique has the same resolution as allele specific sequencing. Sequencing has the advantage of identifying novel polymorphic sites but where all SNP sites have been identified this novel procedure can resolve all alleles and haplotypes and identify novel combinations of polymorphisms. This method is similar in price to direct sequencing and provides a low cost system for direct haplotyping of extended DNA sequences.
One of the major priorities in genome analysis is to determine the joint inheritance and the population-wide associations among contiguous alleles on the same chromosome. This is known as the haplotype structure of a population and it is an essential part of the genetic architecture. The haplotype structure determines the usefulness of closely linked markers in identifying disease-susceptible individuals or genetically superior livestock. It also influences genetic interactions. For example, a polymorphism in a promoter, an enhancer or another regulatory element may interact differently with an allele on the same or on an opposing chromosomal strand.
Either deductive or direct approaches can be used to define haplotypes. The deductive approach infers haplotypes from the inheritance patterns in families or from the observed associations among genotypes [1]. This approach is widely used. However, the algorithms can have error rates between 2 and 48% [1,2].
Direct approaches analyse single chromosomes. The first techniques used cloning and sequencing [3], but this is time-consuming and impractical for large populations. Subsequently, SNP were used to design allele specific primers and the resulting products were then sequenced. This approach was used to haplotype a 6.4 Kb fragment spanning the APOE gene [4] and a 19 Kb portion of the human glucocorticoid receptor [5]. Lo et al [6] used allele-specific forward and reverse primers to haplotype two SNP's <500 bp apart. This work was further developed by Eitan and Kashi [7] who determined the haplotypes of 14 SNP in a 653 bp segment of the chicken HSP 108 gene. While this method allows long regions of DNA to be haplotyped, it is a two stage process, requiring initial sequencing or genotyping to define suitablele SNP that can be used for allele specific PCR. Unfortunately, the fragments that can be analyzed are of limited size, and the dependence on electrophoresis as the end point greatly limits sample throughput. Clearly, a simple, reliable and rapid method for direct haplotyping is urgently needed.
The approach that we have developed has two stages. In the first stage, PCR is performed in the presence of all forward and reverse primers both in solution and linked to the beads. This overcomes some of the difficulties of solid phase PCR [8,9] by allowing in-solution amplification of products, which are subsequently 'kidnapped' to the solid phase. In the second stage, the beads are washed free of non-covalently linked DNA, interrogated by allele specific primer extension with individual fluorescently labeled primers, washed and analyzed. This is a fully automatable procedure with a high degree of internal quality control.
We chose to test this procedure on the DRB1 locus of the sheep major histocompatibility complex. This locus and its homologues is among the most polymorphic in the mammalian genome. In sheep, over 100 alleles have been identified at this locus but additional alleles are believed to exist. The combination of high levels of diversity with unknown alleles provided a rigorous test of our procedure. In addition, sheep are an important commercial species and the DRB1 locus is associated with resistance to nematode infection [10,11], which is a major disease of livestock including sheep [12]. Therefore any results would have both scientific and commercial relevance.
Of the 237 nucleotides examined in exon 2 at the DRB1 locus, 60 (28%) were polymorphic. The majority of polymorphic sites were biallelic with only two nucleotides, but a substantial proportion had three or even four different nucleotides (Figure 1).
Figure 1. Consensus sequence for reported alleles.
The direct haplotyping procedure identified 33 exon 2 alleles at the DRB1 locus in 109 Scottish Blackface Rams. PCR using allele-specific primers and sequencing gave identical results. Both techniques identified one or two exon 2 alleles in all animals. Therefore both techniques appeared to identify all alleles in the population.
The majority of these exon 2 alleles had not been previously identified in this breed of sheep, while two exon 2 alleles had not been previously identified in sheep of any breed. They were assigned Genebank accession numbers FJ213447 and FJ213448. These new alleles both represented novel combinations of nucleotides previously reported to be polymorphic. Both exon 2 alleles have been independently confirmed (Table 1).
Table 1. Alleles identified in Scottish Blackface sheep.
The names of the exon 2 alleles are listed in Table 1 while the frequencies are presented in figure 2. The distribution of exon 2 allele frequencies was very skewed. Three exon 2 alleles (DRB1*0101, *0501 and *0901: Y10248, U00216 and AB017204) accounted for more than 50% of the observations and the vast majority of the homozygosity observed. In contrast, 7 exon 2 alleles occurred in only 2 sheep while 11 exon 2 alleles were present in just one animal.
Figure 2. Frequency chart of the 33 alleles in 109 Scottish Blackface rams.
The fluorescent signal from the amplified products was readily distinguished from the background. The mean number of positive events in the absence of the SNP allele across all primers was only 9 ± 5. In contrast, the number of positive events in the presence of the SNP allele was 195 ± 12. Non-specific amplification was readily distinguished from allele-specific amplification by setting a threshold of 100 events.
A Multiplex double ARMS reaction on solid phase (Trans-Cistor) was used to determine the linkage phase of each pair of polymorphic sites and to resolve complex genotypes into constituent haplotypes. Essentially this technique determines whether the alleles at two linked SNP loci are in a trans or cis relationship. (Trans-Cistor). This approach has the same resolution as allele specific sequencing. In addition, unlike direct sequence based genotyping, it does not require the time-consuming and error prone assignment of alleles from a single polymorphic sequence. However, direct sequencing does have the advantage of identifying novel polymorphic nucleotides but for loci such as the MHC, the vast bulk of the variation is the result of novel combinations of polymorphisms at well-described positions. We have demonstrated that this technique (Trans-Cistor) is capable of identifying such novel exon 2 alleles. Importantly, both of the novel exon 2 alleles have been independently confirmed.
An alternative bead-based method has been developed independently [13]. In this procedure, oligonucleotides specific for SNP allele are attached to differently-coloured fluorescent beads and use to probe amplicons which have been generated from the test genome. This procedure relies on allele-specific hydridisation and like the double ARMS procedure described here allows high-throughput determination of SNP haplotypes.
ARMS based techniques can be prone to both false positive (due to promiscuous priming) and false negative results (due to PCR failure). VOSS addresses these potential problems in a number of ways. Each PCR reaction uses at least four specific primers. When the sample is heterozygous at both SNP pairs, four positive reaction products are produced. Heterozygosity at only one of the pair produces 2 reaction products, whereas homozygosity at both members of the pair produces a single product. Each SNP is tested multiple times within the same reaction. In addition, there is a minimum of two positive fluorescent bead populations for every expected reaction. This inherent quality control greatly aids the confident assignment of haplotype. Such built in redundancy might generally be thought to be expensive. However the cost of the fluorescent beads (£1.60 or $3.00 per test) is in the same order as single sequencing reactions, and with all other reagents and consumables being common to both techniques, Trans-Cistor compares favorably in economic terms. Moreover, it is the potential to automate from PCR set up to haplotype identification that shows the greatest potential advantage.
Solid phase PCR has been reported to have an efficiency less than half that of liquid phase [14]. We have adopted two strategies to address this. As each reaction is represented in both the liquid and solid phase, there is always a more efficient liquid phase reaction, producing template for the solid phase reaction. Additionally, the use of laser detection of fluorescent product on beads, allows detection of extremely small quantities of DNA, far less than could be visualized with ethidium bromide stained electrophoresis gels. Furthermore, while multiplex PCR reactions are prone to produce many spurious products that cannot be easily identified by electrophoresis, the detection system used here only identifies those products with the expected priming site at each end, eliminating the 'multiplex noise'.
PCR based techniques are always limited by the size of the DNA region which can be amplified efficiently and this may be a specific limitation on Solid phase PCR. We have tested this by amplifying portions of exon 14 of the human clotting factor VIII gene. Solid phase amplicons up to 10 Kb could routinely be produced, and amplicons up to 3.5 Kb could be readily produced in multiplex reactions (data not shown), demonstrating the power of the technique to determine haplotype over extended regions.
The double ARMS [6] method is a general approach to determining linkage phase of polymorphic markers. Trans-Cistor offers the ability to automate all the way through to haplotype. Its application extends to all uses of haplotype information including linkage analysis for the diagnosis of genetic diseases. Population studies on the geographical distribution of haplotypes can be performed without pedigree analysis. Haplotype information over extended regions may also be used to study genetic recombination.
The solid phase double ARMS is a robust method that has been applied to the complex DRB1 locus in sheep. However, this method could be used routinely for haplotype determination and could have many diagnostic applications. Potential applications include genotyping of viral strains in mixed infection, chimaerism following bone marrow or stem cell transplantation, and mapping the boundaries of translocations in malignant cell clones.
The Trans-Cistor procedure improves the accuracy with which linkage disequilibrium can be estimated. We compared linkage disequilibrium between SNP at positions 8 and 233. The first method used in individuals whose haplotypes are unknown, uses a composite linkage disequilibrium coefficient [15]. In our sample the most common haplotype was AA with a frequency of 0.31 and a correlation coefficient of 0.23. In contrast, when the directly determined haplotypes were used the correlation coefficient was 0.17. As linkage disequilibrium is widely used to identify genetic markers for disease, the ability to obtain correct estimates in unrelated individuals without testing extended families will be invaluable.
The solid phase double ARMS approach combines exquisite sensitivity with the specificity to detect a minority DNA population. High accuracy results from multiple testing of each SNP and the repeated checking of phase against multiple sites.
We examined 109 rams. All rams were purebred Scottish Blackface rams from 12 Scottish farms in the 'Blackface Elite Sire Reference Scheme'. This is the largest sheep breeding scheme in the UK. Twenty-two different farms have the same breeding objectives and they share and exchange rams every year to facilitate comparisons among lambs from different farms. Over 10,000 lambs are produced every year. Blood sampling was covered by Home Office personal and project licences to M. J. Stear.
Primers for PCR amplification were purchased from MWG (Ebersberg, Germany), Expand long range Taq DNA polymerase from Roche Diagnostics (Burgess Hill, UK) and GoTaq Taq polymerase and dNTPs from Promega (Southampton, UK). Multiplex PCR Buffer was obtained from Point-2-Point Genomics (Edinburgh UK). Luminex beads were purchased from BioRad (Hemel Hempstead, UK). Standard reagents were from SigmaAldrich (Gillingham, UK) or BDH Laboratory Supplies (Poole, UK) in analysis grade, if not otherwise stated.
Assay design
The assay is a multiplex, double Double Amplification Refractory Mutation System (ARMS) based haplotyping method (ARMS [16], also known as PCR amplification of specific alleles or PASA). A short summary of the procedure has been provided (Figure 3) and a diagram illustrating the procedure in Figure 4. Fundamental to this approach is the efficient amplification of product on a defined position on a solid support. Here we have used fluorescently tagged beads, although a conventional microarray could also be used. Up to 100 separately anchored PCR reactions can be multiplexed in the same tube. The assay was developed as a two-stage process. In the first stage, all oligos (forward and reverse) involved in the multiplex reaction are present in the solution phase, and also bound to beads. Thus the solution phase reaction is able to amplify sequences, which then act as templates for the solid phase reaction. After this first stage, the beads are washed, and aliquots of the bead mixture labeled by one round of primer extension with each of the individual fluorescently labeled oligonucleotides. On analysis, products can be detected from every possible combination of forward and reverse oligonucleotides, thus directly identifying the phase of each polymorphism. This process (Trans-Cistor) is covered by patents held by Point-2-Point Genomics: UK & Au patent no 2377220, European Patent EP137 3566, International PCT WO 02081743.
Figure 3. A short summary of the haplotyping procedure.
Figure 4. Diagram of the typing procedure. In 4A, in the first phase, allele specific primers are shown in red and black. The sample is shown as wavy lines in grey and blue. There is a PCR reaction in the supernatant with forward and reverse allele-specific primers. The PCR amplicon is then kidnapped and attaches to one of the reverse primers on the solid phase. In the presence of nucleotides and polymerase a second chain is formed. In 4B, the kidnapped amplicon, which is only held by hydrogen bonding, is released by heating. Washing leaves a covalently bonded single strand. In 4C, a reverse primer is introduced which binds to the covalently attached amplicon. In reality we have all possible forward and reverse primers illustrated as 3 candidate SNP in a row in green, red and grey. In 4D, the results from the separate reactions can be combined to indicate the SNP haplotypes.
Primer design
Primer sequences are listed in Table 2. These were designed with reference to an alignment of the known allele sequences for ovine DBR1 (figure 1). Primers were selected to avoid internal or paired complementarities, and to have melting temperatures of 55°C ± 3°C. For each primer position, oligonucleotides were synthesized, to offer allelic discrimination at the 3' terminal base. Where polymorphic bases were included other than at the extreme 3' end of the sequences these were either synthesized as 'wobbles', or used as part of the allelic discrimination set (see Table 2 legend). For immobilization to beads, oligonucleotides were synthesized with a 5' amino link C12 spacer to minimize steric hindrance [9,17].
Table 2. Sequences of allele specific primers used in this study.
Conjugation to Beads
Luminex (xMAP) suspension array technology is based on polystyrene beads with a diameter of 5.6 μm that are internally dyed with various ratios of two spectrally distinct fluorophores. Thus, an array of 100 different bead sets with specific fluorescent ratios is created. These sets are combined to a suspension array and, due to their unique fluorescence pattern, allow up to 100 different analytes to be measured simultaneously in a single reaction. Conjugation of oligonucleotides to carboxylated microspheres was done by a minor modification of the Luminex protocol [18,19]. Briefly, 5 × 106 unlabeled, carboxylated microspheres, were vortexed in a 1.5 ml microcentrifuge tube, dispersed by sonication for 30 s, centrifuged at 8000 g for 1 min and, after supernatant removal, adjusted to 0.1 M 2-(N-morpholino) ethanesulfonic acid, pH 4.5. An aliquot of 1 nmol 5' amino-modified oligonucleotide was added, followed by 2.5 μl of fresh 1-ethyl-3-(3-dimethyaminopropyl)carbodiimide-HCl (Perbio Science, Cramlington UK) from a 10 mg/ml stock. After 1 h at room temperature the microspheres were repeatedly washed by centrifugation and stored at 4°C in the dark. A control oligonucleotide, with 5' amino link C12 spacer and 3'fluorescent label was conjugated with each batch of reactions to estimate conjugation efficiency.
Phase-1 reaction conditions
Reaction conditions, with MgCl2 concentration, primer concentrations, annealing temperature, and additives (e.g., bovine serum albumin and DMSO, TEMAC) were tested extensively to optimize the PCR reactions. Optimal conditions were 1× Multiplex PCR buffer, 2 nM MgCl2, 200 μM of each dNTP, 25 mU/μL of GoTaq DNA Polymerase, and sterile deionized water to the appropriate volume. Optimum primer concentrations were determined empirically. Each reaction included 2,000 beads of each specificity. After an initial denaturation at 95°C for 3 min, there were 10 cycles of 95°C for 15 s, 60°C for 20 s, 70°C for 45 s, and 20 cycles of 10 cycles of 95°C for 15 s, 55°C for 20 s, 70°C for 45 s. On completion of thermal cycling, the beads were washed 3 times by dilution in 200 μl TE (10 mM Tris, pH 7.4; 1 mM EDTA) and 30 s incubation at 95°C.
Phase-2 reaction conditions
For the second phase reaction, the beads were resuspended in a 25 μL reaction volume containing 1× Multiplex PCR buffer, 1 nM MgCl2, 50 μM of each dNTP, 25 mU/μL of GoTaq DNA Polymerase and 0.05 μM specific labeled primer. The reaction mixture was then incubated at 95°C for 15 s, and 65°C for 30 s. Beads were subsequently washed 3 times by dilution in 1× Multiplex PCR buffer and 30 s incubation at 75°C.
Analysis of beads
Following the final wash, the beads were resuspended in 0.5 ml TE, and analysed by flow cytometry on a FACSCalibur Flow cytometer (BD Biosciences). A single bead population was gated on forward and side scatter. 100,000 events from this population were then analysed on FL3 and FL4 to discriminate each of the bead populations. These individual bead populations were then gated, and analysed for FL1 and FL2 for the reporter fluorescence. A positive fluorescence was defined as being at least four fold higher than background fluorescence (measured in un-reacted beads). For a reaction to be scored as positive, both the beads representing that reaction (ie with the forward or reverse primer in the solid phase) had to be positive.
PCR of Sheep DNA for sequencing
DNA was extracted from jugular blood samples from 109 sheep, using standard phenol/chloroform methods. The second exon of DRB1 was amplified using a semi-nested PCR technique. The first round of PCR was performed with primers ERB3 (5'-GGAATTCCTCTCTCTGCAGCACATTTCCT-3') and HL031 (5'-TTTAAATTC GCGCTCACCTCGCCGCT-3') [20]. 100 ng of genomic DNA was amplified by PCR in a total volume of 25 μl of PCR buffer (1× multiplex buffer, 1.5 mM MgCl2, and 120 μM dNTP), to which 0.2 mM each primer, and 25 mU/μL of GoTaq DNA Polymerase, and sterile deionized water to the appropriate volume had been added. Reactions were performed under the following conditions: 5 min at 94°C, followed by 15 cycles of 94°C for 15 s, 50°C for 30 s, and 72°C for 60 s, with final extension at 72°C for 5 min. We amplified 3 μl of the resulting mixture with primers ERB3 and SRB3 (5'-AAGTCGACCGCTGCACAGTGAAACTC-3') for the second round of PCR. The conditions for the second round of PCR were one cycle for 5 min at 94°C, followed by 30 cycles of 94°C for 30 s, 60°C for 30 s, and 72°C for 60 s with final extension at 72°C for 10 min. PCR products were checked by agarose gel electrophoresis, before being sequenced with primers ERB3 and SRB3. Forward and reverse sequences were aligned with Sequence Navigator Software (ABI), and where heterozygous bases were identified, a third sequencing reaction using an appropriate allele specific oligonucleotide (Table 2) was performed. Analysis of all sequences allowed haplotypes to be assigned.
Conventionally, a locus is the site of a gene on a chromosome while an allele is an alternative form of a gene and a haplotype represents a contiguous set of genes on a chromosome. The size of a gene can vary from a single nucleotide in an SNP allele to over 150,000 nucleotides in the Huntington's Disease locus. Some loci such as the MHC class II genes contain many SNP even within a single exon. Therefore a protein-coding allele can also be considered as an SNP haplotype. Usually, the context makes matters clear but to minimize potential confusion, we occasionally refer to SNP alleles, SNP haplotypes and protein-coding alleles.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
DS carried out the molecular genetic studies and drafted the manuscript. MJS participated in the design of the study, carried out statistical analyses and revised the initial draft. Both authors read and approved the final manuscript.
This study was financed by a Department of Trade and Industry SMART award. Brendan Hamill assisted in the design of this study and Point-2-Point Genomics provided access to the patent.
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Cape Western Railway Society, Goodwood
Cape Western Railway Society
- Street:
- P.O. Box 491
- Postcode/City:
- 7459 Goodwood
- Province:
- Western Cape
- Phone:
- 021 691 0027021 691 0027
- Website:
- capewestrail.co.za
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The Cape Western branch of the Railway Society of Southern Africa meets on the first Tuesday of each month, except January, at the club house of the Western Cape Live Steamers in Parow, at 19:30 where members enjoy a programme of lectures, films, videos, slides, discussions and other items of railway interest.
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City: Holmdel
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The sights, sounds, and smells of rural Monmouth County in the 1890s have been re-created on 9 acres at Historic Longstreet Farm in Holmdel Park, Holmdel. Interpreters in period dress show both...
City: Holmdel
62 Longstreet Rd. Holmdel, NJ 07733
The Holmes-Hendrickson House, built circa 1754, reflects a merger of English and Dutch style houses and is a testament to the cultural heritage of its occupants. The exterior façade and interior...
City: Middletown
137 Kings Hwy. Middletown, NJ 07748
Marlpit Hall, built circa 1756, was home to the prominent loyalist Taylor family at the time of the Revolution. Edward Taylor endured house arrest for his political position and the family lost...
City: Lincroft
765 Newman Springs Rd. Lincroft, NJ 07738
The Monmouth Museum, founded in 1963 as a Museum of Ideas, presents changing exhibitions in art, history and science to educate and entertain while providing a destination for creative expression...
City: Navesink
PO Box 332 Navesink, NJ 07752
The New Jersey Lighthouse Society (NJLS) was formed in 1989 as a state chapter of the United States Lighthouse Society. NJLS assists in the restoration and preservation of lighthouses in New Jersey...
City: Holmdel
1 Memorial Ln. Holmdel, NJ 07733
Holmdel is the site of the New Jersey Vietnam Veterans' Memorial, Vietnam Era Museum and Educational Center....
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PAIR OF GEORGE III-STYLE CARVED MAHOGANY LIBRARY ARMCHAIRS . Circa 1900. 38 x 28-1/2 x 29 inches (96.5 x 72.4 x 73.7 cm). ... (Total: 2 Items)
2013 February 23-24 Estate Auction - Dallas #5129
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Heritage Auctions - Design District Annex
1518 Slocum Street
Dallas, TX
Circa 1900
38 x 28-1/2 x 29 inches (96.5 x 72.4 x 73.7 cm)
With carved arms and cabriole legs terminating in pad feet.
Condition Report*:The chairs present well; one has wear to the upholstered crest rail and one has missing casters and loss to front left foot, sturdy overall.
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Population estimates released by the U.S. Census Bureau show Franklin County — home to Columbus — recorded by far the most births in the state last year, 18,200. The birth rate of 15.21 per 1,000 residents in Franklin beat out Hamilton County — home to Cincinnati — which had a birth rate of 13.73.
The Columbus Dispatch reported Ohio on the whole lost almost 7,400 people to migration last year. Almost half of that loss occurred in Cuyahoga County, home to Cleveland.
Three-fourths of the state's counties had a net loss of residents because of people moving out of Ohio.
Hocking County in southeast Ohio had the biggest population loss — 2.34 percent.
Statistics show Franklin County is closing in on Cuyahoga County and could become Ohio's most populated by the 2020 census.
Ohio had more than 11.5 million residents last year. The state's population was up 17,777 residents over 2012.


text:
Lil Bow Wow's biological father is Alfonso Moss, a man he hasn't had a relationship with except for through the media. His stepfather is Rodney Caldwell.
Lil Bow Wow was born Shad Gregory Moss on March 9, 1987, in Columbus, Ohio. His stage name was given to him by Snoop Dog when he performed as an opening act for the legendary rapper.
His contentious relationship with his birth father has been played out in public through messages and interview snippets. In 2012, his father famously called him out for his parenting tactics, telling Bow Wow (who has dropped the Lil from his name) not to be the kind of dad he was.
Lil Bow Wow born Shad Gregory Lamar Moss never met his father and w...
He is a rapper and a actor
Bow Wow never met his biological father. He was raised by his mother Theresa Caldwell & step dad Rodney Calswell. He was born on Mar 9, 1987.
he's master p's son.


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|ISSN 1088-6826(online) ISSN 0002-9939(print)
On the classification
Abstract | References | Similar Articles | Additional Information
Abstract: We present some results associated with the existence of central configurations (c.c.'s) in the classical gravitational -body problem of Newton. We call a central configuration of five bodies, four of which are coplanar, a central configuration (p.c.c). It can be shown that there are only three types of p.c.c.'s, admitting one or more planes of symmetry, viz. (i) the case where the planar bodies lie at the vertices of a regular trapezoid, (ii) the case where the bodies lie at the vertices of a kite-shaped quadrilateral, and (iii) the case where the bodies lie at the vertices of a rectangle. In this paper we classify all p.c.c.'s with a rectangular base and, in fact, prove that there is only one such c.c., namely, the square-based pyramid with equal masses at the corners of the square. The classification of all p.c.c.'s satisfying either (i) or (ii) will be discussed in subsequent papers.
Retrieve articles in Proceedings of the American Mathematical Society with MSC (1991): 70F15, 70F10
Retrieve articles in all journals with MSC (1991): 70F15, 70F10
Additional Information


text:
- Detail Images
A ridge along the hull of the Old Town Cayuga 130 Kayak provides stability in choppy waters, while the bow and stern hatches store lunch, extra layers, and camera equipment. Since no one wants to paddle all day on a seat that feels like a medieval torture instrument, Old Town made the 13-foot Cayuga seat with a cushiony, adjustable back and bottom. Padded thigh braces keep the cockpit rim from digging into your legs. Cross-lock covers on the Cayuga 130's bow and stern hatches help keep water out of the storage bulks, and deck bungees hold maps and a windbreaker.
Share your thoughts
What do you think of the
Old Town Cayuga 130 Kayak
? Share a...
Steve Creek
Does this kayak come with a skirt, and if...
Does this kayak come with a skirt, and if not, what skirts that you carry would fit?
finlay king
is this a quick turning kayak for fast...
is this a quick turning kayak for fast water
I love winter
No. It is a recreational kayak meant to be paddled in mild conditions on flatwater.
I've taken mine through class II and III and it's done fine with a skirt. It's a tough boat!
Jeremy quesinberry
This will be my first kayak looks really...
This will be my first kayak looks really nice i wana know how it will do in the river ?
Thomas Jones
It depends on what river you are kayaking.
It's longer size makes it more Ideal for lakes or flat rivers.
Don't expect it to preform well in rapids. But if you plan on taking it down light rapids then It should work fine.
All in all, you should only buy this for 90% lake travel. If you plan on taking it down a lot of rivers Old town sells a short, cheap, but excellent kayak called the otter. It works very well down easy rapids or just casual short trips.
I am trying to find info on the Old Town...
I am trying to find info on the Old Town Loon 12 ft. Kayak. I found one on craigslist. $250 10 years old. tripple hull. I don't know if it is a good boat or a good deal. Wondering if I should buy a new Kayak...Someone told me Old Town is one of the best brands. thank you, I look forward to your input.
I own a loon 13 foot kayak I use it mostly for over night trips on river & lakes Very stable,I am buying a cayuga 13o soon but will keep my loon for over night trips you should buy the loon for250 good beginner kayak
I have large feet...will there be enough...
I have large feet...will there be enough room in this kayak or should i go with the 14 ft ?
Angus Bohanon
I think your feet will fit in this just fine. If they don't, the 14 footer isn't going to be any thicker.


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Henry Taylor
Position: DT
Height: 6-2 Weight: 295 lbs.
Born: November 29, 1975 in Broward County, FL
High School: Barnwell
College: South Carolina (school history)
Weighted Career AV (100-95-...): 0 (14747th overall since 1950)
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Output includes all games from 1920 to 2014.


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50 years ago (1959), the Reds were swept in a doubleheader at Crosley Field vs. the Pirates--losing the opener 6-2, and the nightcap 14-11.
First Game
Bill Virdon cf
Dick Groat ss
Smoky Burgess c
Rocky Nelson 1b
Bob Skinner lf
Bill Mazeroski 2b
Don Hoak 3b
Roman Mejias rf
Vern Law p
Johnny Temple 2b
Vada Pinson cf
Jerry Lynch lf
Frank Robinson 1b
Gus Bell rf
Frank J. Thomas 3b
Ed Bailey c
Roy McMillan ss
Bob Purkey p
WP: Vern Law (5-2)
LP: Bob Purkey (5-6)
HR: PIT - Smoky Burgess 2 (5), Don Hoak 2 (4).
In Game 1, Vern Law pitched a complete-game eight-hitter to get the win for the Pirates.
Second Game
Bill Virdon cf
Dick Groat ss
Danny Kravitz c
Ted Kluszewski 1b
Bob Skinner lf
Bill Mazeroski 2b
Don Hoak 3b
Roman Mejias rf
Al Jackson p
Johnny Temple 2b
Vada Pinson cf
Pete Whisenant rf
Frank Robinson lf
Walt Dropo 1b
Dutch Dotterer c
Frank J. Thomas 3b
Roy McMillan ss
Don Newcombe p
WP: Elroy Face (7-0)
LP: Bob Mabe (3-2)
HR: PIT - Bob Skinner 2 (10), Don Hoak (5), Smoky Burgess (6). CIN - Dutch Dotterer (1), Roy McMillan (6).
In Game 2, Pirates starter Al Jackson made his major-league debut.


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we were very impresed of daniel his local restaurant,great food the best we ever taste in Italy,
JP v Dierendonck
We ate here twice during our stay in Assisi and both meals were wonderful. It is a small place just a block from the main road you take from Assisi to the highway. It is delightful inside, the service is great, the prices are very reasonable, and food is some of the best we had on our 3 month trip...
Nice people, fresh local food en service with the extra mile.
Eat there 3 of the 4 days we were there.
Great service, excellent food, and wonderful atmosphere. All local, artisinal food, reasonably priced.
We have eaten here the last two evenings and both times were the top two meals we have had on our two week holiday so far. It's a pleasure to find an unassuming restaurant that genuinely finds pleasure in treating people well and serving slightly unusual great food. Very good value - three courses for four people, wine for two,...
Located directly across from the Santa Maria degli Angeli Cathedral, this cute and clean restaurant is an excellent selection. The staff was friendly and the service was prompt. An English menu is available. The mixed cheese starter was simple and nice as was the lamb main course. Prices are reasonable for the standard offered. Air conditioned indoor seating offered along...
Had we heard of this restaurant, or read the TripAdvisor reviews, we would headed straight there during our trip to Assisi and the Umbria region. But it was completely by chance, after visiting Santa Maria degli Angeli, that we walked in off the street - and sound ourselves enjoying a truly memorable experience. Food is divine, welcome is wonderful, service...
Outstanding! Everything was great, the service, excellent food and wine! Not expensive at all, really good value for money.
We enjoyed our lunch here so much that we decided to go back for supper. The menu is not really big but every dish is made to perfection. The ingredients come from the owners farm in Norcia. I have never tasted such wonderful prosciutto and cold cuts - all sliced right in the store portion of the restaurant. The ingredients...
Very friendly service, nice food, dinner for 2 with 2 courses with wine, water and cover €54 fair value. Great location by the Duomo especially in the evening. Restaurant produces and sells a lot of its produce.
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text:
1 Shouts - 361 Scrobbles
4 N.F. stands for 4 Night Flames.
This eurodance project with Kurtis Blow released the single "I Wanna Be Your Lover in 1995.
4 Night Flames on Last.fm.
User-contributed text is available under the Creative Commons By-SA License and may also be available under the GNU FDL.
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National Network to End Domestic Violence's Technology Summit
July 27, 2015 - July 30, 2015
San Francisco, California
Domestic Violence & Sexual Assault Training
September 22, 2015 - September 25, 2015
Orlando, Florida
Domestic Violence & Sexual Assault Training
November 2, 2015 - November 5, 2015
Reno, Nevada
Domestic Violence & Sexual Assault Training
December 8, 2015 - December 11, 2015
Las Vegas, Nevada
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Luxury Rental Car in Phoenix, Arizona
Luxury cars > > Luxury cars USA > > Luxury cars Phoenix, Arizona >>
Looking for a great sports car rental in Phoenix? Sixt offers a wide range of great luxury cars to choose from at Phoenix Airport, so get ready to explore Arizona in style. Sixt is looking forward to handing over the keys to your luxury vehicle!
Sixt Sports Cars in Phoenix
Sixt offers luxury vehicles from car brands like Mercedes-Benz, BMW, Cadillac, Ford, and so much more. With Sixt, you can also get a convertible or turbo version of our vehicles. To get a sports car make sure you book the car category LFAR, XFAR, XTAR, or PTAR.
Sixt rent a car has some of the lowest prices on luxury cars in Phoenix. Fly into Sky Harbor Airport, pick up a sports car and drive through the desert, cruise the city, or check out spring training. No matter what your plans are in Phoenix, having a Sixt sports car will make your trip even more magical.
Example Luxury Cars in Phoenix
|Mercedes-Benz CLA (PCAR)
|Cadillac ATS (LCAR)
|BMW 5 Series (XCAR)
|Cadillac SRX (LFAR)
|Ford Mustang (STAR)
|Mercedes-Benz SLK (PTAR)
More Information about our Phoenix Location
- 1805 E Sky Harbor Circle South 85034 Phoenix
- Phone # +1-602-4255736
- Fax # +1-602-5327863
To find out this location and get more information about traveling to Phoenix, Click Here.
Sixt Requirements for Premium Car Rentals
- Must be 25 years of age
- Must have a credit card
- Deposit will be taken once the vehicle is picked up and will be returned once vehicle is returned
- Must of held driver's license for minimum of one year


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You can now invite anyone to meet at your favorite Starbucks.
Limited-time feature
30 year old woman | Wichita Falls, TX, USA | Seeking men 18-35 within 50 miles
Insert witty and meaningful eye-catching statement that will captivate and entertain (I hate writing these).
I am constantly full of energy. I love coming up with ideas and creating things, whether it be writing something or making art work. I am working towards graduate school, aiming to get my Masters in English. Eventually I hope to teach. I am a sucker for someone who is quick witted and funny (I wanted to marry Peter Venkman from Ghostbusters when I was a child and I adore anything Joss Whedon dreams up). I want someone who is up for trying new things but understands if I want to just chill at home. Having two jobs is exhausting and makes it hard to get out and do a lot of things, but getting out every once and awhile is awesome. Ideally, I would like someone who likes science fiction, supernatural shows, and comic books, but if not that's okay (it opens up the possibility of new experiences to see what that other person likes). I like video games, not so much sports-based ones, but the rest are fun. I love hearing stories and meeting new people.
Coffee and conversation , Dining out , Movies/Videos , Museums and art , Music and concerts , Exploring new areas , Performing arts , Shopping/Antiques , Travel/Sightseeing , Video games
Aerobics, Dancing, Other types of exercise, Running, Swimming, VolleyBall, Walking / Hiking, Weights / Machines
I exercise 1-2 times per week.
I have Cats
I like Birds, Dogs, Exotic Pets, Fish, Horses, Other
Three cats, one is a rescue.
Middle of the Road
I'd like to visit Ireland, New York City, London, New England, Greece.
Midwestern State University, Wichita Falls, TX
TV/Movies: Supernatural, The Vampire Diaries, Grey's Anatomy, the History Channel, Ghostbusters, Blade. Music: Evanescence, Florence The Machine, Rob Zombie, Disturbed, Breaking Benjamin. Comic book: Spider-Man, X-Men, Batman
Movies, listening to music, online gaming, writing, working in Adobe Photoshop, watching TV, working out at fitness center, creating art work, karaoke
The Hunger Games Trilogy, World War Z, Anita Blake Novels, Comic books, News on Yahoo.com, contemplating picking up the Girl with the Dragon Tattoo trilogy,
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What she is looking for
|5'8" (172cms)
|5'10" (177cms) to 6'4" (193cms)
|Body type:
|No preference
|No preference
|Auburn / Red
|No preference
|No Way
|No Way
|Social Drinker
|Never, Social Drinker, Moderately
|Administrative / Secretarial
|No preference
Secretary and web master
|I'll tell you later
|No preference
|Never Married
|No preference
|Have kids:
|Wants kids:
|No preference
|White / Caucasian
|No preference
Irish (Primarily), Scottish, British, Black Dutch, Native American (Cherokee/Choctaw), German
|I'll tell you later
|No preference
|Bachelors degree
|High school, Some college, Associates degree, Bachelors degree, Graduate degree, PhD / Post Doctoral


text:
Beautifully Simple: School Bus Turned Minimal Mobile Home
You could make a strong case for this vehicle being barely recognizable as such. The dimensions, fenestration and over spatial configuration give good clues ...
Heart of the Home Laid Open: Intimate Kitchen Portraits
Would you ever allow a stranger to come into your home, open every cabinet and cupboard in your kitchen, and photograph whatever he finds? Artist Erik Klein ...
Transformer Theater: Deserted House, Upcycled in Disguise
This incredible transforming theater, built from salvaged pieces of a deserted home, is more than meets the eye. A community theater in disguise, its contents ...
Wheel House: Circular Hobo Home is a Rolling Circus Marvel
In this strange circus performance piece, two travelers turn architectural conventions on end, moving an offbeat nomadic home through a comedic narrative via ...
Cute, Kitschy, Cool: 12 Clever Chinese Desk Fan Designs
Intended for low-cost mass production but displaying a wide variety of clever conceptual designs, these 12 Chinese desk fans may blow but they sure don't suck.
Vertical Garage: Hide Cars Below or Lift Your Ride to the Sky
There are all kinds of houses designed to showcase your prized luxury vehicle or custom cruiser, but few can boast so blatant a mechanism to quite literally ...
Nail House: Holdout Building Had Highway Built Around It
China is full of strange stories of so-called 'nail houses' - homes of people who refuse to move to make way for a large development project.
Decor on Demand: 14 3D-Printed Home Accents
Liven up your home decor with fun 3D-printed accents like lamps, espresso cups, planters and kits to make three-dimensional geometric objects out of pencils.
Borrowed from Libraries: Mobile Shelving for Modular Rooms
To save space, libraries employ a rolling stack system that allows access to only one or a few aisles needed at one time. How about the same in homes or ...
Ding Dong! A Dozen Delightfully Daffy Doorbell Designs
Ring the bell or knock on the door? It's one of life's conundrums to be sure, but these dozen delightfully daffy doorbell designs can help button it down.
- Categories
- Architecture
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text:
Updated 04/14/2014
The cool, forested grounds here lie close to the center of town but seem far away. The Austrian owners lavish you with lots of personal attention. The bungalows on stilts are simply furnished but contain hot-water baths, mosquito nets over the beds (you'll need them), and hammocks on the porch. There are also two fully furnished houses available for short- or long-term rental. Great coffee is included in the room rate; a huge buffet breakfast is extra. You'll receive a discount if you pay with cash.
180 m south of bridge at entrance to town, Puerto Viejo de Talamanca, 70403, Costa Rica
Website: www.cocolocolodge.com
Hotel Details:
Updated 04/14/2014
No Thanks


text:
Need to know: CV cliches Why you can`t find a jobHF interviews
By Rumman Ahmed of Dow Jones Newswires
Fortis Healthcare India said a unit has acquired an 85% stake in Singapore outpatient diagnostic and molecular imaging chain RadLink-Asia Pte. for S$62.9 million (2.48 billion rupees).
By Kenan Machado of Dow Jones Newswires
Fortis Healthcare (India) Ltd. said it has completed a $665 million acquisition of its founders' Singapore-based firm Fortis Healthcare International Pte. Ltd.
By Rumman Ahmed of Dow Jones Newswires
Citigroup Global Markets Mauritius Pvt. Ltd. has sold 2.56 million shares of Fortis Healthcare (India) Ltd. for about 221.36 million rupees ($4.17 million).
Deal Journal India is a one-stop destination following deals, deal-makers, private equity and venture-capital activity in India. The real-time blog is updated throughout the day with breaking news, exclusive analysis, commentary and profiles. Edited by The Wall Street Journal's Vibhuti Agarwal, Deal Journal India features contributions from the South Asia bureaus of The Wall Street Journal and Dow Jones Newswires in Mumbai and Delhi as well as bureaus around the world. Reach our Editors at firstname.lastname@example.com or follow Deal Journal India on Facebook and Twitter.
FINS has become a part of DHI in order to improve your job search experience. eFinancialCareers will provide you with the best possible service, the best job opportunities and editorial content and the best talent. With our long track record of helping finance professionals develop their career, from graduating to landing top positions in the industry, we will continue to deliver.
For technology jobs outside the financial industry users can visit Dice.com


text:
Welcome to a suite so stylish, so comfortable, and so spacious, you'll never want to leave. The largest of Hotel Adagio's suites, the 900-square foot Stone Suite sits on Floor Sixteen and boasts amazing views of the city. With a master bedroom complete with a king-size bed, a luxurious bath and a separate living room with decorative fireplace, this suite is perfect for entertainers and VIPs alike.
- Breathtaking views of the city
- Exterior facing view of the city
- Wi-Fi Internet service
- In-room dining service
- Luxe cotton linens
- 32" HD LCD TV with Showtime
- Premium sound system with docking station
- Ample work space with ergonomic chair
- Minibar with refrigerator
- Electronic safe
- Luxurious green-certified bath products
- Hairdryer
- Individual heating and cooling control
- Fully lighted framed vanity
- Iron and ironing board
- Double-pane windows
- Air conditioning
- Ceiling fan


text:
PHILADELPHIA (AP) ŌĆö Authorities say 15 cabins were destroyed and seven others were damaged by fire Monday morning on the Neshoba County Fairgrounds in east central Mississippi.
By PAUL SIMS
By COLLEEN MCCARTHY
By STEVEN NALLEY
The eaglets were living on borrowed time from the moment they were born.
The coaches of the Starkville High School tennis team believe the program is on the verge of some special things in the not so distant future.
The Starkville High School golf team is looking to put everything together for a run at a state championship on Tuesday.
EUPORA ŌĆö Avonell T. Hughes, 87, of Kilmichael, died Friday, May 6, 2011 in the University Hospital, Jackson.
The Starkville Community Market kicked off their season bright and early Saturday morning.
JACKSON (AP) ŌĆö Longtime state Sen. Jack Gordon of Okolona, known as a master of the state budgeting process, died Saturday night at his home, according to Senate leaders.
By DANNY P. SMITH
Jalan Catledge seized the opportunity when it came time to help the Starkville Yellowjackets at the Class 6A North State track meet Saturday afternoon.


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Pork, Plums, Scallions, and Jalapeños? Grill 'Em All in One Sandwich!
When I get out of New York City and actually have a chance to grill, I don't just want to cook the obvious stuff on the grill, I want to cook everything. Case in point: This grilled pork sandwich with a grilled plum chutney and miso-cabbage slaw. It's been held over the flames, from top to bottom. More


text:
All the Land to Hold Us (Hardcover)
Usually Ships in 1-5 Days
A masterfully crafted novel of seekers that spans three generations set amidst the harsh terrain of West Texas.
About the Author
RICK BASS's fiction has received O. Henry Awards, numerous Pushcart Prizes, awards from the Texas Institute of Letters, fellowships from the National Endowment for the Arts and the Guggenheim Foundation, among others. Most recently, his memoir "Why I Came West" was a finalist for a National Book Critics Circle Award.


text:
- Pearl Strands
- Gold
- 7-8mm 18"
- Gift Box
Nice necklace for the price 01/05/2013
This review is from: The Pearl Outlet FNCR8AAP-YG Cranberry Red Freshwater Pearl Necklace - 8mm, 14k Gold Clasp, AAA, 18"
This is a nice necklace looks good for the color and came with a nice package. Pearls look nice and polished.
None so far.
Did you find this review helpful? Yes / No Thanks for the valuable feedback you provided!


text:
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Paddleboard Plaid 10" Shorts (# T87214)
- 55% linen, 45% Tencel® lyocell
- Dry clean only
- Inseam: 10"
- Four pockets
- Imported
Rated 4.6 out of 5Â by 7 reviewers.
Rated 5 out of 5Â by Beachtime Exactly what we expected. Great fit This shorts were exactly what we expected. They are true to size and travel well. August 25, 2014
Rated 5 out of 5Â by Collie12 Awesome shorts These shorts are the most comfortable shorts that I have ever purchased. Size was accurate as advertized. I will definitely order more shorts from TB. July 27, 2014
Rated 5 out of 5Â by TClayborne love the shorts and material Great shorts for casual wear. I look forward to buying more. July 25, 2014
Rated 2 out of 5Â by jwalken Slim fit.....what? Loved everything about these except the fit. The description does not say these are slim fit. There is a little tag on one of the belt loops that says standard fit, aka for slim. I'm not a slim guy, so when they sold these in 44 I was happy. When they arrived the waist fit fine, but the legs and butt do not. I wish TB sold more size 44 shorts or more inventory for the ones they do, as when they do they are sold out in days, often before I can find them. June 6, 2014
Rated 5 out of 5Â by ChicagoSunshine Great shorts! The fabric, fit and look of these shorts are terrific. They can be worn with short or long sleeve button shirts, polos and t-shirts - very versatile! My husband loves them!! June 4, 2014
Rated 5 out of 5Â by cama1959 Great Shorts I just love the shorts fit nice , it is fresh and perfect for the beach April 7, 2014
Rated 5 out of 5Â by NOSPF COMFORTATBLE & LIGHTWEIGHT This is another TB winner. A lightweight and breathable short that is very comfortable. This short is a bit longer than most of my other TB shorts, but they look great. I have worn them with t-shirts, linen shirts, and polo shirts and they look good with all of them! February 28, 2014
1-7 of 7


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How To Get Healthy, Shiny Hair
Are you tired of dull, lifeless hair? If your hair is giving you trouble, you're not the only one. The road to long and luscious hair is a difficult one. But with a few simple tips, you can get healthy, shiny hair in no time. Different lengths and styles require different maintenance so just because your friend's routine works for her doesn't mean it'll work for you too. Be sure to tailor your haircare routine [...]
Healthy Lunch Box Ideas for Kids
Many parents don't realize how important it is to pack a healthy lunch for their kids. Food is our energy, our fuel, and with out sufficient energy how do you expect your kids to learn or even pay attention in school? It is becoming more and more clear to the world the negative effects of unhealthy eating habits. Thanks to Dr. Oz, the authors of books such as Wheat Belly, Eat to Live and the [...]


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This is, without a doubt, the wildest, most erotic and exciting video ever produced for big babies and diaper lovers everywhere.
If your fantasy is to be helplessly turned into a baby by a dominant woman, then you won't want to miss this one.
Lee places an ad in a newspaper looking for a lady willing to play fantasy "baby games", but the woman who answers his ad actually wants to turn him into a real, total, complete baby FOREVER. The woman forces Lee to wet his pants right in the middle of a busy restaurant and feeds him a bottle, much to the amusement of the waitress who has warmed it for her.
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text: StoryCorps A Father, a Son, and a 10-Cent Mistake September 29, 2006 To say that Samuel Black's father worked would be like saying that Bill Gates made money. But he found time to teach his son lessons. "He was a very stern disciplinarian," Samuel Black says -- often, all his father had to do was look at his sons, and his meaning was clear. Listen Loading… Playlist Download Embed Embed Close embed modal <iframe src="http://www.npr.org/player/embed/6161284/6161293" width="100%" height="290" frameborder="0" scrolling="no">


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35 year old woman | Jamaica Plain, MA, USA | Seeking women 20-45 within 60 miles of Boston, MA
Don't let me be the one who got away!
I'm a writer by trade, but writing a self-description is surprisingly difficult for me. I'm vibrant, caring, and creative (don't let the lack of creativity in this description fool you!) I can be prone to sarcasm - a healthy amount, I think, and pretty standard for a writer - but overall, I am very kind and considerate individual; I tend to value a person's capacity for kindness above all other traits. I'm a fan of pretty much all fiction (mystery, horror, hefty character-driven fiction), cult TV shows, and really cheesy horror movies; my idea of a good Saturday night is curling up on the couch, preferably with one of the aforementioned distractions, but don't let this introvertedness fool you. I'm also interested in live music, theater, and enjoying conversation with good friends over a few drinks. Not to damage my nerd-cred or anything, but I am also interested in athletics. I used to play volleyball and softball, and I am interested it taking up both again. I took up fencing in college, a hobby I'm particularly proud of since there aren't really a whole lot of fencers out there (for those of you familiar with the sport, I'm trained in all three weapons, but foil and epee are my two favorites). I'd also like to say that I am a pool shark, but alas, I am just a minnow. Other hobbies I have include traveling, to pretty much anywhere. I've spent a good deal of time traveling across Europe and the eastern portions of the United States/Canada, but I have yet to get to Asia, South America, or even the west coast. I have an obsessive desire to collect stamps in my passport (not that I am obsessive or anything :) ; I simply like seeing - and experiencing - new cultures. Ideally, the woman I'm looking for is strong, supportive, and confident - but who of us out there isn't looking for this woman? It's impossible to sum her up in only a few words. I don't think we could ever describe someone's character so easily, especially when the qualities I admire the most can take so many different forms. I think what's most important is that she is passionate about what she loves, and is willing, every now and then, to curl up for a cheesy movie.
Book club , Coffee and conversation , Dining out , Movies/Videos , Museums and art , Music and concerts , Exploring new areas , Performing arts , Playing sports , Political interests , Travel/Sightseeing , Video games , Wine tasting
Baseball, Billiards / Pool, Running, Soccer, Swimming, Tennis / Racquet sports, VolleyBall, Walking / Hiking
I exercise 1-2 times per week.
I like Birds, Cats, Dogs, Exotic Pets
Local/independent music venues, pool, coffee houses, tapas bars and ethnic eateries, theater
I'm a fan of virtually any kind of movie (although not big on raunchy comedy or romantic comedy). With TV, I love the cult favorites (Star Trek, Buffy the Vampire Slayer). I'm an avid reader: mystery, literary, horror - anything with good characters.
Movies/theater, museums, reading, wine-tasting (more for the company than the wine)
Amy and Isabelle; The Shining; The Giant's House
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What she is looking for
|5'2" (157cms)
|4'9" (144cms) to 6'4" (193cms)
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|No preference
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I am a writer and a teacher. Both can be extremely rewarding. I like knowing (or hoping) that I can make a difference!
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|Graduate degree
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I don't really have that much of a problem with soldered on RAM any more, as it's the way so much of the small profile laptops are these days. But 4 gig of RAM with Yosemite? There are going to be a bunch of disappointed non-computer savvy people who thought they'd be getting a typically snappy Mac. That chintzy RAM starts caching around with THAT pokey drive? Why even bother marketing that configuration? Can it even run for a week without imploding?
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Originally written on Pro Sports Daily | Last updated 8/2/12
As well as the Tampa Bay Rays' starters have been doing, Alex Cobb didn't want to be the one to mess it up. So after working out of messy, 25-pitch, bases-loaded first inning, he turned in a solid seven-inning outing as the Rays capped their cross-country road trip with a 4-1 win over the A's at Oakland. Tampa Bay wound up 6-3 on the trip. "I think it's kind of like hitting; it's just a contagious thing," said Cobb, who improved to 5-8 with a 4.60 ERA. "You see one pitcher doing good, you want to match him. You definitely don't want to be 'that' guy, to be the weak link in the rotation." Cobb, who has been filling in for injured Jeff Niemann, had two straight solid starts. On Friday in Anaheim, he allowed three runs in one inning and nothing in his other six. "That was more what he looked like last year in July when he came up to help us out," manager Joe Maddon said. "Better command of his fastball, he threw some really good curveballs, his changeup had more depth to it. ...
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Lifestyle Changes to Manage Brain Tumors
American Brain Tumor Association website. Available at: http://hope.abta.org/site/PageServer.
Harrison TR, Fauci AS. Harrison's Principles of Internal Medicine. 14th ed. New York, NY: McGraw-Hill; 1998.
The Whole Brain Atlas website. Available at: http://www.med.harvard.edu/AANLIB/home.html.
Revision Information
- Reviewer: Mohei Abouzied, MD
- Review Date: 05/2014 -
- Update Date: 05/28/2014 -
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When cleaning your eye, wipe from the inside (next to the nose)
toward the outside. Use a clean surface for each wipe so that drainage being
cleaned away is not rubbed back across the eye. If tissues or wipes are used,
make sure they are put in the trash right away. If
washcloths are used to clean the eye, put them in the laundry right away so
that no one else picks them up or uses them. After wiping your eye, wash your
hands to prevent the pinkeye from spreading.


text:
Although the club have not yet officially announced the deal, manager Manuel Pellegrini confirmed on Saturday night that the England midfielder, who signed for City's sister club New York City FC this summer, will join up with the team on Wednesday.
"As you know Frank has signed a contract with New York City and will start working here [the United States] in January as the season starts in March," Pellegrini said.
"From next Wednesday he will start working with our team and will play for us. He will work with the squad and be a member of the squad. He will fight for a position. We have a lot of players in his position. He is one player more."


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Smith College
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KevinGutting: Women's Air Force (WAF) veteran Fern Hohman Robinson, 90, to be featured in Gazette story by @EmmaKolchin. GazetteNET (link)
LaurieLoisel: William Ryder indicted 9 more counts larceny over $250, 1 life insurance fraud; Susan Ryder indicted life insurance fraud, larceny over $250
GazetteUMass: RT @BobMcGovernJr: J.T. Blyden No Longer with #UMass Football Program (link) via @MaroonMusket
StevePfarrer: Fantastic show at the Clark Art Institute on Vincent van Gogh; also a smaller show on "Whistler's Mother." (link)
mikemoranDHG: We're releasing our player of the year and all-star teams. 1st up is boys tennis. Girls tennis goes Friday with girls track slated for Sat.


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20th November 2014
Woman who says she is girlfriend of Michael Phelps reveals she was born intersex 5
2:18 PM — A woman who claims to be the girlfriend of Olympic swimmer Michael Phelps has revealed that she was born intersex.
21st March 2014
As 'God Hates Fags' founder Fred Phelps dies, Twitter mistakenly mourns Olympic swimmer Michael Phelps 8
12:30 PM — Do you know the difference between the late long-time homophobe Fred Phelps and US Olympic swimmer Michael Phelps? Apparently these Twitter users don't.
10th September 2012
London: Gays urged to 'come' Out to Swim 25
4:18 PM — After a summer of Olympic sporting fever, the capital's LGBT community is being urged to hit the pool. Out to Swim's Rory Desch shares the experience of one of its members, Micke Masood, who's found a whole new lease of life with the gay amateur swim club.


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Dr. Anne Gurnack is professor emerita and former chair of the Department of Politics, Law and Philosophy at the University of Wisconsin-Parkside. Her teaching areas include American politics, public policy and public administration. She has been a visiting professor at UWM and the University of Gdansk, Poland since her retirement. She has been a frequent guest on MPTV's Fourth Street Forum and WGTD Public Radio.
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Respond to Review of Planet India
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Nolan Ryan says Josh Hamilton is giving at bats away
Jul 24, 2012, 9:12 AM EDT
Early this season it looked like it'd be impossible for the Rangers to let Josh Hamilton walk when he hits free agency this fall. Then Hamilton started to skid and he hasn't stopped. In June he hit .223/.318/.436. In July he's hitting .172/.242/.345. He has a grand total of seven homers in those two months.
Nolan Ryan was asked about it on a radio show the other day and this is what he had to say:
You're right that some of his at bats aren't very impressive from the standpoint that he doesn't work deep into the count, he's swinging at a lot of bad pitches, he just doesn't seem to be locked in at all. So what you're hoping is that his approach will change and he'll start giving quality at bats because there's a lot of those at bats that he just gives away. One of the things I've always commented on is I can't ever say that I ever saw Henry [Hank] Aaron give an at bat away.
He speaks truth. And maybe I'm reading way too much into this, but I get the sense than he's doing more than just describing what's wrong with Hamilton. Rather, he's also laying the groundwork for letting Hamilton walk after this year without Rangers fans going crazy. Reminding them that while Hamilton may be talented, he's not the sort of superstar [cough] Hank Aaron [cough] that only comes around once in a lifetime and, yes, we will survive without him.
- ninjasports - Jul 24, 2012 at 9:24 AM
Sports are ultimately a business, and letting Hamilton go is obviously a business decision because he is a great player
- suhisabeast90 - Jul 24, 2012 at 9:31 AM
Can I have one of those bats he's giving away? I have a pine tar addiction.
- sabathiawouldbegoodattheeighthtoo - Jul 24, 2012 at 9:34 AM
While Hamilton has tremendous potential, the Rangers offense could certainly endure without him. They would be better off locking up Napoli to a far more modest extension and nabbing a less-expensive OF in free agency (Victorino? Swisher? Hunter?) or from their farm.
They should not be giving Hamilton an ARod contract or they could end up with, well, an ARod contract.
- beefytrout - Jul 24, 2012 at 9:52 AM
I certainly understand the thinking behind preparing for Hamilton to not be a Ranger in 2013, but wouldn't it make more sense to trade him and get something valuable in return?
- Phillies Homer - Jul 24, 2012 at 10:00 AM
Maybe if they weren't in first place and arguably the most complete team in the AL.
- beefytrout - Jul 24, 2012 at 10:07 AM
Things aren't going *that* well for them… a month ago they had a run differential that dwarfed everyone else. Before last night, the Yankees had more or less caught them. They just lost Lewis for the season, have Feliz still on the DL, Holland just came off it, Oswalt's back is acting up, and Darvish is still figuring things out. They're using Tanner Scheppers in the bullpen, for crying out loud.
There is lots that could be done to improve the Rangers. And the laaa alberts are not going away.
- js20011041 - Jul 24, 2012 at 9:53 AM
Dave Cameron wrote a nice article about this at Fangraphs a little while back saying pretty much the same thing. He really does need to tighten up. It's probably not a good idea to take Vlad Guerrero's approach at the plate unless you can put the bat on the ball like Vlad Guerrero.
- crali - Jul 24, 2012 at 11:17 AM
This reminds me of what the Yankees did to Bernie, Posada, & Jeter. One might think that as a former player Ryan would find a different way of doing this – especially when it comes to someone who has issues but then it's always about the money, isn't it?
- vallewho - Jul 24, 2012 at 12:50 PM
[cough] speculation [cough]
- artisan3m - Jul 24, 2012 at 1:54 PM
Why is Hamilton still batting 3rd in the lineup? In fact, with Gentry's BA along with his speed and consistency, why is Hamilton still IN the lineup? Maybe Josh needs to fall off the wagon again. Right after his last encounter with a bottle, he was a holy terror.
Wash's loyalty is admirable but Josh has become a liability at the plate. The message is clear ~ there is no penalty on the Rangers for non-performance. That's not good.
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Register now for free, or sign in with any of these services:
Rest in Peace, Rocky.
With love,
Lorraine Modzelewski
Congratulations Midland Beach residents. You came together as a community and beat the odds.
WilliamWallace.. Absolutely, they'd allow that purchase! Heck, the government (Obama) won't even call a terrorist a terrorist. How's that for mud in your eye?
Both parents should be held accountable.. father sucks, but mother is no better if she allows such a monster near her child. The child should be placed in a caring foster home.
From the article it appears as though a lot of Staten Island children are being abused one way or another... What's in the water? This Moss guy should never see the light of day... keep him confined and away from children.
Sad thing about it is that she'll probably win the lawsuit.
While the watress should have been more aware of the placement of the carafe within the reach of an infrant, it is the mother's responsiblity to insure the safty of her child. Ever since the McDonalds lawsuit there has been an increase in hot coffee claims across the nation. I would hate to think that in times of economic struggges some of these accidents are... not accidents?
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Schengen Area Passport Validity Requirements
I just saw this posted on TripAdvisor: SCHENGEN AREA: PASSPORT VALIDITY REQUIREMENT On 26 June 2013, the European Union published regulation EU 610/2013, modifying Regulation EU 562/2006 (Schengen Borders Code). According to the regulation, third-country nationals entering the Schengen area will be required to hold a travel document valid for at least three months after the intended date of departure from the territory of the Member States. The requirement will enter into force on 19 July 2013. IATA is currently seeking clarification on the upcoming changes and effective dates with the authorities of individual Schengen Member states. The official date is tomorrow, but it looks like individual countries can set their own dates to enforce the rule. edited to add the regulation document.


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Did Lindsay Lohan tamper with her SCRAM bracelet the night of the MTV Movie Awards after party?
Sources say that although her test for alcohol consumption came back negative, Lindsay was indeed drinking that night.
Lindsay Lohan Tests Negative for Alcohol
RadarOnline.com is reporting that Lindsay's alcohol level was between .03 and .04 from midnight to 3AM on June 6th.
"There was absolutely no doubt that Lindsay was drinking, and the report that the alcohol monitoring service provided to Judge Revel was very detailed and extremely reliable," a source close to the situation said.
There are also reports that Lindsay's SCRAM bracelet appeared to have been tampered with. "The (SCRAM) report also says that Lohan's SCRAM bracelet had indeed been tampered with, and this is one of the reasons that Judge Revel determined that Lindsay violated terms of her bail," the source added.
Lindsay's rep says she took a urine test that indicated she had no alcohol in her system. The time of the test will be compared to the SCRAM data by both the prosecution and Lindsay's defense.
Lindsay's Ex is Worried About Her
Despite reports that the probation department has cleared Lindsay, Judge Revel still isn't 100% convinced.
Her next hearing in July 6th so stay tuned! Maybe this new assistant of hers can keep her on the straight and narrow! Apparently the new guy was an intern at a big agency in town, and was offered the job. We don't know which job sounds worse!


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text: U.S. Doctors May See Payment Cut for Medicare Patients June 27, 2008 Doctors who treat Medicare patients will likely see a 10.6 percent cut in payments starting Tuesday. The move follows the Senate's failure to pass a Medicare bill on Thursday night. The House had earlier approved a similar measure. Listen Loading… Playlist Download Embed Embed Close embed modal <iframe src="http://www.npr.org/player/embed/91975518/91975502" width="100%" height="290" frameborder="0" scrolling="no">