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Proteins: Structure, Function, Bioinformatics
Structure of a cupin protein Plu4264 from Photorhabdus luminescens subsp. laumondii TTO1 at 1.35 Å resolution
[Apr 2015]
Structure of the yeast Bre1 RING domain
[Apr 2015]
Monoubiquitination of histone H2B at Lys123 in yeast plays a critical role in regulating transcription, mRNA export, DNA replication and the DNA damage response. The RING E3 ligase, Bre1, catalyzes monoubiquitination of H2B in concert with the E2 ubiquitin conjugating enzyme, Rad6. The crystal structure of a C-terminal fragment of Bre1 shows that the catalytic RING domain is preceded by an N-terminal helix that mediates coiled-coil interactions with a crystallographically related monomer. Homology modeling suggests that the human homologue of Bre1, RNF20/RNF40, heterodimerizes through similar coiled coil interactions. This article is protected by copyright. All rights reserved.
Solution structure and properties of AlgH from pseudomonas aeruginosa
[Apr 2015]
In Pseudomonas aeruginosa, the algH gene regulates the cellular concentrations of a number of enzymes and the production of several virulence factors, and is suggested to serve a global regulatory function. The precise mechanism by which the algH gene product, the AlgH protein, functions is unknown. The same is true for AlgH family members from other bacteria. In order to lay the groundwork for understanding the physical underpinnings of AlgH function, we examined the structure and physical properties of AlgH in solution. Under reducing conditions, results of NMR, electrophoretic mobility, and sedimentation equilibrium experiments indicate AlgH is predominantly monomeric and monodisperse in solution. Under non-reducing conditions intra- and intermolecular disulfide bonds form, the latter promoting AlgH oligomerization. The high-resolution solution structure of AlgH reveals alpha/beta-sandwich architecture fashioned from ten beta strands and seven alpha helices. Comparison with available structures of orthologues indicates conservation of overall structural topology. The region of the protein most strongly conserved structurally also shows the highest amino acid sequence conservation and, as revealed by hydrogen-deuterium exchange studies, is also the most stable. In this region, evolutionary trace analysis identifies two clusters of amino acid residues with the highest evolutionary importance relative to all other AlgH residues. These frame a partially solvent exposed shallow hydrophobic cleft, perhaps identifying a site for intermolecular interactions. The results establish a physical foundation for understanding the structure and function of AlgH and AlgH family proteins and should be of general importance for further investigations of these and related proteins. This article is protected by copyright. All rights reserved.
Computational characterization of the chemical step in the GTP hydrolysis by Ras-GAP for the wild-type and G13V mutated Ras
[Apr 2015]
The free energy profiles for the chemical reaction of the guanosine triphosphate hydrolysis GTP + H2O GDP + Pi by Ras-GAP for the wild-type and G13V mutated Ras were computed by using molecular dynamics protocols with the QM(ab initio)/MM potentials. The results are consistent with the recent measurements of reaction kinetics in Ras-GAP showing about two-order reduction of the rate constant upon G13V mutation in Ras: the computed activation barrier on the free energy profile is increased by 3 kcal/mol upon the G13V replacement. The major reason for a higher energy barrier is a shift of the "arginine finger" (R789 from GAP) from the favorable position in the active site. The results of simulations provide support for the mechanism of the reference reaction according to which the Q61 side chain directly participates in chemical transformations at the proton transfer stage. Proteins 2015. © 2015 Wiley Periodicals, Inc.
Deformability in the cleavage site of primary MicroRNA is not sensed by the double-stranded RNA binding domains in the microprocessor component DGCR8
[Apr 2015]
The prevalence of double-stranded RNA (dsRNA) in eukaryotic cells has only recently been appreciated. Of interest here, RNA silencing begins with dsRNA substrates that are bound by the double-stranded RNA binding domains (dsRBDs) of their processing proteins. Specifically, processing of microRNA (miRNA) in the nucleus minimally requires the enzyme Drosha and its dsRBD-containing cofactor protein, DGCR8. The smallest recombinant construct of DGCR8 that is sufficient for in vitro dsRNA binding, referred to as DGCR8-Core, consists of its two dsRBDs and a C-terminal tail. Because dsRBDs rarely recognize the nucleotide sequence of dsRNA, it is reasonable to hypothesize that DGCR8 function is dependent on recognition of specific structural features in the miRNA precursor. Previously, we demonstrated that non-canonical structural elements that promote RNA flexibility within the stem of miRNA precursors are necessary for efficient in vitro cleavage by reconstituted Microprocessor complexes. Here we combine gel shift assays with in vitro processing assays to demonstrate that neither the N-terminal dsRBD of DGCR8 in isolation, nor the DGCR8-Core construct, are sensitive to the presence of non-canonical structural elements within the stem of miRNA precursors, or to single-stranded segments flanking the stem. Extending DGCR8-Core to include an N-terminal heme-binding region does not change our conclusions. Thus, our data suggest that while the DGCR8-Core region is necessary for dsRNA binding and recruitment to the Microprocessor, it is not sufficient to establish the previously observed connection between RNA flexibility and processing efficiency. This article is protected by copyright. All rights reserved.
Structural mapping of the coiled-coil domain of a bacterial condensin and comparative analyses across all domains of life suggest conserved features of SMC proteins
[Apr 2015]
The structural maintenance of chromosomes (SMC) proteins form the cores of multisubunit complexes that are required for the segregation and global organization of chromosomes in all domains of life. These proteins share a common domain structure in which N- and C- terminal regions pack against one another to form a globular ATPase domain. This "head" domain is connected to a central, globular, "hinge" or dimerization domain by a long, antiparallel coiled coil. To date, most efforts for structural characterization of SMC proteins have focused on the globular domains. Recently, however, we developed a method to map interstrand interactions in the 50-nm coiled-coil domain of MukB, the divergent SMC protein found in γ-proteobacteria. Here, we apply that technique to map the structure of the Bacillus subtilis SMC (BsSMC) coiled-coil domain. We find that, in contrast to the relatively complicated coiled-coil domain of MukB, the BsSMC domain is nearly continuous, with only two detectable coiled-coil interruptions. Near the middle of the domain is a break in coiled-coil structure in which there are three more residues on the C-terminal strand than on the N-terminal strand. Close to the head domain, there is a second break with a significantly longer insertion on the same strand. These results provide an experience base that allows an informed interpretation of the output of coiled-coil prediction algorithms for this family of proteins. A comparison of such predictions suggests that these coiled-coil deviations are highly conserved across SMC types in a wide variety of organisms, including humans. Proteins 2015. © 2015 Wiley Periodicals, Inc.
Crystal structure of the human odorant binding protein, OBPIIa
[Apr 2015]
Human odorant-binding protein, OBPIIa, is expressed by nasal epithelia to facilitate transport of hydrophobic odorant molecules across the aqueous mucus. Here, we report its crystallographic analysis at 2.6 Å resolution. OBPIIa is a monomeric protein that exhibits the classical lipocalin fold with a conserved eight-stranded β-barrel harboring a remarkably large hydrophobic pocket. Basic residues within the four loops that shape the entrance to this ligand-binding site evoke a positive electrostatic potential. Human OBPIIa shows distinct features compared with other mammalian OBPs, including a potentially reactive Cys side chain within its pocket similar to human tear lipocalin. Proteins 2015. © 2015 Wiley Periodicals, Inc.
Genome-wide computational determination of the human metalloproteome
[Apr 2015]
Accurate prediction of protein function in humans is important for understanding biological processes at the molecular level in biomedicine and drug design. Over a third of proteins are commonly held to bind metal, and ∼10% of human proteins, to bind zinc. Therefore, an initial step in protein function prediction frequently involves predicting metal ion binding. In recent years, methods have been developed to predict a set of residues in 3D space forming the metal-ion binding site, often with a high degree of accuracy. Here, using extensions of these methods, we provide an extensive list of human proteins and their putative metal ion binding site residues, using translated gene sequences derived from the complete, resolved human genome. Under conditions of ∼90% selectivity, over 900 new human putative metal ion binding proteins are identified. A statistical analysis of resolved metal ion binding sites in the human metalloproteome is furnished and the importance of remote homology analysis is demonstrated. As an example, a novel metal-ion binding site involving a complex of a botulinum substrate with its inhibitor is presented. On the basis of the location of the predicted site and the interactions of the contacting residues at the complex interface, we postulate that metal ion binding in this region could influence complex formation and, consequently, the functioning of the protein. Thus, this work provides testable hypotheses about novel functions of known proteins. Proteins 2015; 83:931–939. © 2015 Wiley Periodicals, Inc.
Crystal structure of a novel two domain GH78 family α-rhamnosidase from Klebsiella oxytoca with rhamnose bound
[Apr 2015]
The crystal structure of the GH78 family α-rhamnosidase from Klebsiella oxytoca (KoRha) has been determined at 2.7 Å resolution with rhamnose bound in the active site of the catalytic domain. Curiously, the putative catalytic acid, Asp 222, is preceded by an unusual non-proline cis-peptide bond which helps to project the carboxyl group into the active centre. This KoRha homodimeric structure is significantly smaller than those of the other previously determined GH78 structures. Nevertheless, the enzyme displays α-rhamnosidase activity when assayed in vitro, suggesting that the additional structural domains found in the related enzymes are dispensible for function. This article is protected by copyright. All rights reserved.
The conservation profile of a protein bears the imprint of the molecule that is evolutionarily coupled to the protein
[Apr 2015]
The conservation profile of a protein is a curve of the conservation levels of amino acids along the sequence. Biologists are usually more interested in individual points on the curve (namely, the conserved amino acids) than the overall shape of the curve. Here, we show that the conservation curves of proteins bear the imprints of molecules that are evolutionarily coupled to the proteins. Our method is based on recent studies that a sequence conservation profile is quantitatively linked to its structural packing profile. We find that the conservation profiles of nucleic acid (NA) binding proteins are better correlated with the packing profiles of the protein-NA complexes than those of the proteins alone. This indicates that a nucleic acid binding protein evolves to accommodate the nucleic acid in such a way that the residues involved in binding have their conservation levels closely coupled with the specific nucleotides. This article is protected by copyright. All rights reserved.
Differential dynamics of RAS isoforms in GDP- and GTP-bound states
[Apr 2015]
RAS subfamily proteins regulates cell growth promoting signaling processes by cycling between active (GTP-bound) and inactive (GDP-bound) states. Different RAS isoforms, though structurally similar, exhibit functional specificity and are associated with different types of cancers and developmental disorders. Understanding the dynamical differences between the isoforms is crucial for the design of inhibitors that can selectively target a particular malfunctioning isoform. In this study, we provide a comprehensive comparison of the dynamics of all the three RAS isoforms (HRAS, KRAS, and NRAS) using extensive molecular dynamics simulations in both the GDP- (total of 3.06μs) and GTP-bound (total of 2.4μs) states. We observed significant differences in the dynamics of the isoforms, which rather interestingly, varied depending on the type of the nucleotide bound and the simulation temperature. Both SwitchI (residues 25-40) and SwitchII (residues 59-75) differ significantly in their flexibility in the three isoforms. Furthermore, Principal Component Analysis showed that there are differences in the conformational space sampled by the GTP-bound RAS isoforms. We also identified a previously unreported pocket, which opens transiently during MD simulations, and can be targeted to regulate nucleotide exchange reaction or possibly interfere with membrane localization. Further, we present the first simulation study showing GDP destabilization in the wild-type RAS protein. The destabilization of GDP/GTP occurred only in 1/50 simulations, emphasizing the need of guanine nucleotide exchange factors (GEF) to accelerate such an extremely unfavorable process. This observation along with the other results presented in this paper further support our previously hypothesized mechanism of GEF-assisted nucleotide exchange. This article is protected by copyright. All rights reserved.
Improved energy bound accuracy enhances the efficiency of continuous protein design
[Apr 2015]
Flexibility and dynamics are important for protein function and a protein's ability to accommodate amino acid substitutions. However, when computational protein design algorithms search over protein structures, the allowed flexibility is often reduced to a relatively small set of discrete side-chain and backbone conformations. While simplifications in scoring functions and protein flexibility are currently necessary to computationally search the vast protein sequence and conformational space, a rigid representation of a protein causes the search to become brittle and miss low-energy structures. Continuous rotamers more closely represent the allowed movement of a side chain within its torsional well and have been successfully incorporated into the protein design framework to design biomedically relevant protein systems. The use of continuous rotamers in protein design enables algorithms to search a larger conformational space than previously possible, but adds additional complexity to the design search. To design large, complex systems with continuous rotamers, new algorithms are needed to increase the efficiency of the search. We present two methods, PartCR and HOT, that greatly increase the speed and efficiency of protein design with continuous rotamers. These methods specifically target the large errors in energetic terms that are used to bound pairwise energies during the design search. By tightening the energy bounds, additional pruning of the conformation space can be achieved, and the number of conformations that must be enumerated to find the global minimum energy conformation is greatly reduced. This article is protected by copyright. All rights reserved.
Structural analysis of the polo-box domain of human Polo-like kinase 2
[Apr 2015]
Polo-like kinases (Plks) are the key regulators of cell cycle progression, the members of which share a kinase domain and a polo-box domain (PBD) that serves as a protein-binding module. While Plk1 is a promising target for antitumor therapy, Plk2 is regarded as a tumor suppressor even though the two Plks commonly recognize the S-pS/T-P motif through their PBD. Herein, we report the crystal structure of the PBD of Plk2 at 2.7 Å. Despite the overall structural similarity with that of Plk1 reflecting their high sequence homology, the crystal structure also contains its own features including the highly ordered loop connecting two subdomains and the absence of 310-helices in the N-terminal region unlike the PBD of Plk1. Based on the three-dimensional structure, we furthermore could model its interaction with two types of phosphopeptides, one of which was previously screened as the optimal peptide for the PBD of Plk2. This article is protected by copyright. All rights reserved.
RBRIdent: An algorithm for improved identification of RNA-binding residues in proteins from primary sequences
[Apr 2015]
Rapid and correct identification of RNA-binding residues based on the protein primary sequences is of great importance. In most prevalent machine-learning-based identification methods, however, either some features are inefficiently represented, or the redundancy between features is not effectively removed. Both problems may weaken the performance of a classifier system and raise its computational complexity. Here, we addressed the above problems and developed a better classifier (RBRIdent) to identify the RNA-binding residues. In an independent benchmark test, RBRIdent achieved an accuracy of 76.79%, Matthews correlation coefficient of 0.3819 and F-measure of 75.58%, remarkably outperforming all prevalent methods. These results suggest the necessity of proper feature description and the essential role of feature selection in this project. All source data and codes are freely available at http://18.104.22.168/RBRIdent. This article is protected by copyright. All rights reserved.
Complete characterization of the mutation landscape reveals the effect on amylin stability and amyloidogenicity
[Apr 2015]
Type-II diabetes is believed to be partially aggravated by the emergence of toxic amylin protein deposits in the extracellular space of the pancreas β-cells. Amylin, the regulatory hormone that is co-secreted with insulin, has been observed to misfold into toxic structures. Pramlintide, an FDA approved injectable amylin analog mutated at positions 25, 28, and 29 was therefore developed to create a more stable, soluble, less-aggregating, and equipotent peptide that is used as an adjunctive therapy for diabetes. However, because Pramlintide is not ideal, researchers have been exploring other amylin analogs as therapeutic replacements. In this work, we assist the finding of optimal analogs by computationally revealing the mutational landscape of amylin. We computed the structure energies of all possible single-point mutations and studied the effect they have on amylin stability and amyloidogenicity. Each of the 37 amylin residues was mutated in silico into the 19 canonical amino acids and an energy function computing the Lennard–Jones, Coulomb and solvation energy was used to analyze changes in stability. The mutation landscape identified amylin's conserved stable regions, residues that can be tweaked to further stabilize structure, regions that are susceptible to mutations, and mutations that are amyloidogenic. We used the single-point mutational landscape data to generate estimations for higher-order multiple-point mutational landscapes and discovered millions of three-point mutations that are more stable and less amyloidogenic than Pramlintide. The landscapes provided an explanation for the effect of the S20G and Q10R mutations on the onset of diabetes of the Chinese and Maori populations, respectively. Proteins 2015. © 2015 Wiley Periodicals, Inc.
The unique N-terminal insert in the ribosomal protein, phosphoprotein P0, of Tetrahymena thermophila: Bioinformatic evidence for an interaction with 26S rRNA
[Mar 2015]
Phosphoprotein P0 (P0) is part of the stalk complex of the eukaryotic large ribosomal subunit necessary for recruiting elongation factors. While the P0 sequence is highly conserved, our group noted a 15-16 residue insert exclusive to the P0s of ciliated protists, including Tetrahymena thermophila. We hypothesized that this insert may have a function unique in ciliated protists, such as stalk regulation via phosphorylation of the insert. Almost no mention of this insert exists in the literature, and although the T. thermophila ribosome has been crystallized, there is limited structural data for Tetrahymena's P0 (TtP0) and its insert. To investigate the structure and function of the TtP0 insert, we performed in silico analyses. The TtP0 sequence was scanned with phosphorylation site prediction tools to detect the likelihood of phosphorylation in the insert. TtP0's sequence was also used to produce a homology model of the N-terminal domain of TtP0, including the insert. When the insert was modeled in the context of the 26S rRNA, it associated with a region identified as expansion segment 7B (ES7B), suggesting a potential functional interaction between ES7B and the insert in T. thermophila. We were not able to obtain sufficient data to determine whether a similar relationship exists in other ciliated protists. This study lays the groundwork for future experimental studies to verify the presence of TtP0 insert/ES7 interactions in Tetrahymena, and to explore their functional significance during protein synthesis. This article is protected by copyright. All rights reserved.
BCL::MP-Fold: Membrane protein structure prediction guided by EPR restraints
[Mar 2015]
For many membrane proteins, the determination of their topology remains a challenge for methods like X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy. Electron paramagnetic resonance (EPR) spectroscopy has evolved as an alternative technique to study structure and dynamics of membrane proteins. The present study demonstrates the feasibility of membrane protein topology determination using limited EPR distance and accessibility measurements. The BCL::MP-Fold (BioChemical Library membrane protein fold) algorithm assembles secondary structure elements (SSEs) in the membrane using a Monte Carlo Metropolis (MCM) approach. Sampled models are evaluated using knowledge-based potential functions and agreement with the EPR data and a knowledge-based energy function. Twenty-nine membrane proteins of up to 696 residues are used to test the algorithm. The RMSD100 value of the most accurate model is better than 8Å for twenty-seven, better than 6Å for twenty-two and better than 4Å for fifteen out of twenty-nine proteins, demonstrating the algorithms ability to sample the native topology. The average enrichment could be improved from 1.3 to 2.5, showing the improved discrimination power by using EPR data. This article is protected by copyright. All rights reserved.
Key challenges for the creation and maintenance of specialist protein resources
[Mar 2015]
As the volume of data relating to proteins increases, researchers rely more and more on the analysis of published data, thus increasing the importance of good access to these data that vary from the supplemental material of individual papers, all the way to major reference databases with professional staff and long-term funding. Specialist protein resources fill an important middle ground, providing interactive web interfaces to their databases for a focused topic or family of proteins, using specialised approaches that are not feasible in the major reference databases. Many are labours of love, run by a single lab with little or no dedicated funding and there are many challenges to building and maintaining them. This perspective arose from a meeting of several specialist protein resources and major reference databases held at the Wellcome Trust Genome Campus (Cambridge, UK) on the 11th and 12th of August 2014. During this meeting some common key challenges involved in creating and maintaining such resources were discussed, along with various approaches to address them. In laying out these challenges, we aim to inform users about how these issues impact our resources and illustrate ways in which our working together could enhance their accuracy, currency, and overall value. This article is protected by copyright. All rights reserved.
Method for identification of rigid domains and hinge residues in proteins based on exhaustive enumeration
[Mar 2015]
Many proteins undergo large-scale motions where relatively rigid domains move against each other. The identification of rigid domains, as well as the hinge residues important for their relative movements, is important for various applications including flexible docking simulations. In this work, we develop a method for protein rigid domain identification based on an exhaustive enumeration of maximal rigid domains, the rigid domains not fully contained within other domains. The computation is performed by mapping the problem to that of finding maximal cliques in a graph. A minimal set of rigid domains are then selected, which cover most of the protein with minimal overlap. In contrast to the results of existing methods that partition a protein into non-overlapping domains using approximate algorithms, the rigid domains obtained from exact enumeration naturally contain overlapping regions, which correspond to the hinges of the inter-domain bending motion. The performance of the algorithm is demonstrated on several proteins. This article is protected by copyright. All rights reserved.
Structure and dynamics of DRD4 bound to an agonist and an antagonist using in silico approaches
[Mar 2015]
Human dopamine receptor D4 (DRD4), a member of G-protein coupled receptor (GPCR) family, plays a central role in cell signaling and trafficking. Dysfunctional activity of DRD4 can lead to several psychiatric conditions and, therefore, represents target for many neurological disorders. However, lack of atomic structure impairs our understanding of the mechanism regulating its activity. Here, we report the modeled structure of DRD4 alone and in complex with dopamine and spiperone, its natural agonist and antagonist, respectively. To assess the conformational dynamics induced upon ligand binding, all-atom explicit solvent molecular dynamics simulations in membrane environment were performed. Comprehensive analyses of simulations reveal that agonist binding triggers a series of conformational changes in the transmembrane region, including rearrangement of residues, characteristic of transmission and tyrosine toggle molecular switches. Further, the trajectories indicate that a loop region in the intracellular region––ICL3, is significantly dynamic in nature, mainly due to the side-chain movements of conserved proline residues involved in SH3 binding domains. Interestingly, in dopamine-bound receptor simulation, ICL3 represents an open conformation ideal for G protein binding. The structural and dynamical information presented here suggest a mode of activation of DRD4, upon ligand binding. Our study will help in further understanding of receptor activation, as acquiring structural information is crucial for the design of highly selective DRD4 ligands. Proteins 2014; 83:867–880. © 2014 Wiley Periodicals, Inc.


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The Phantom Menace at 15 was originally published as a postscript to my Q&A column - I'm reprinting it here as its own beast because it got a pretty good reaction and there's no reason to bury it in the back pages. Here it is, with a couple of tweaks. Thank you for your kind indulgence.
It's time for Q&A! Want to know some great other toy figure sites? Sure, who doesn't? And what are the best versions of more of your favorite Star Wars characters as action figures? Let's tackle that too. All this and a Phantom Menace at 15 rant. Read on!
Since you probably never heard of it, the passing of Star Wars: Attack Squadrons before an actual release won't mean much to you. The free-to-play game has been in beta testing, and now according to this Facebook post it won't be doing much of anything. Having joined 1313 and Vernost
Reported everywhere including the official site, Gareth Edwards (Godzilla, Monsters) will direct the 2016 Star Wars movie. What is about? Well, nobody knows. But we do know Gary Whitta will write it, and he has worked on The Book of Eli as well as The Walking Dead video games.
Welcome to Q&A! Do you want 6-inch scale vehicles for your Black Series collection? Well... it might be expensive. And how about paint applications - how can you remove excess paint, if it's showing up? Finally, let's talk about the changing world of Hasbro's boy action toy universe. New products are out there, but are they things you want? Not necessarily. Read on!
Let's talk Yoda's jacket in Q&A this week! We'll also talk about new Disney toys, as that's in this season. And how about more Black Series stand issues? Oh, that's a doozy. My mailbag is empty, so read on and send in some questions for next time!
Last weekend Disney Stores rolled out new Star Wares for May 4th, but a couple weeks earlier a few of us were in California for a press event showcasing new toys including Disney Store Plush. These aren't the same as the items currently sold by Comic Images, some are larger, some are smaller, and they're all quite different - creatures, robots, and aliens are on deck from Star Wars and The Empire Strikes Back.
There's more, read on!
Has Mustafar frozen over? Sponsor Entertainment Earth posted two assortments - Hot Wheels Star Wars cars and Track Sets - with no pictures as of yet, but a September release date. Mattel's first step into a larger world should prove exciting, as they have made die-cast metal vehicles from numerous licenses in many forms.
Hasbro officially releases photos and confirms the upcoming SDCC exclusive Black Series Jabba the Hutt Throne Room (including packaging details) and Black Series 6 inch Yoda. Check out all the visual goodness after the jump!
The headline pretty much says it all - click over! Tom Kane Reveals The Black Series Yoda 6-Inch Figure.
This week in Q&A: What's up with Saga Legends? Well, not much yet. And how much Expanded Universe are we saving? Some! Customization comes up too, so let's bag on the Etsy people. All this and more - read on! (And submit your questions for next week.)
Continuing Star Wars Day celebrations, our friends at Funko are giving away FOUR Pop! Prototypes from the latest assortment!The winner will receive Pop! Prototypes of the following characters: The Emperor, Biker Scout, Bossk, and Luke Skywalker Hoth.
The wait is finally over! You can now view the full Star Wars Rebels trailer courtesy of StarWars.com's YouTube Channel.
Wrapping up their May The 4th week, Sideshow concept art previews their forthcoming Premium Format Boba Fett and Darth Vader! Look for more product and pre-order info soon!

subset: commoncrawl


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Watch here:
What's Fly Day like? Click to check out this video.
(please note that Fly Day is a weather-dependent activity. No rain date is scheduled)]]>
The day broke with some fog in the outlining areas which prevented a few visiting and participating aircraft to fly-in but that did not hamper the start of Fly Day as scheduled with our first flight just after 9 AM. The sky was blue till the eye could see with a CAVOK forecast for the entire day! A total of 16 aircraft and +40 volunteers helped make Fly Day one of the best on record. As the last flight touched down at 17:15 pilots had taken up 313 people and helped raise over $11,000 for The Rotary Club of Ottawa!
Hundreds of hours were spent organizing this event but in the end it would not have been such a success without all of the volunteer pilots, marshallers and passenger escorts. Special thanks also go out to the MacDonald-Cartier International Airport Authority, Nav Canada, Ottawa Flying Club Management, OFC Dispatch, Frank Schilder (Fly Day Load Master), Rob Schwartz and team (Fly Day Air Bosses), Paul Wagner (Lead Marshall), Peter Luff (Lead Passenger Escort), Charlene Mathias who surveyed the patiently waiting public, and also the numerous Algonquin College students who participated.
Enjoy the remainder of the fall flying season. Stay tuned for news of next year's event!
Julie Marion and Marc Desjardins
Fly Day Event Coordinators]]>
We will be providing weather and wait time estimates by Twitter and text message. For more info on Fly Day, click on the ABOUT tab above.
Hope to see you there!]]>


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|* Some accounts: [[http://linkedin.com/in/tehnick|LinkedIn]], [[https://github.com/tehnick|Github]], [[https://www.ohloh.net/accounts/tehnick|Ohloh]], [[https://www.transifex.com/accounts/profile/Tehnick/|Transifex]]
|* Some accounts: [[http://linkedin.com/in/tehnick|LinkedIn]], [[https://github.com/tehnick|Github]], [[https://openhub.net/accounts/tehnick|OpenHub]], [[https://www.transifex.com/accounts/profile/Tehnick/|Transifex]]
Boris Pek
FOSS developer, Debian Developer
Email: <tehnick AT SPAMFREE debian DOT org> (main), <tehnick-8 AT SPAMFREE mail DOT ru> (reserve)
XMPP address: <tehnick AT SPAMFREE jabber.spbu DOT ru>
Debian member information
Activity in mailing lists: Gmane, Google (part 1), Google (part 2)
Launchpad activities
- IM (XMPP) and VoIP (SIP, Jingle) clients software
File sharing (mostly p2p protocols like Direct Connect and BitTorrent)
- Software for Numerical Simulations
- Cross-platform software
- QA work
Personal web-site (mostly in Russian)
Build scripts for maintaining packages
- Among others I am currently subscribed to these mailing lists:


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Lecture note posting harms academic publishing, publishers claim
July 4, 2011 | 11:51 pm
By Chris Meadows
The closure of a half-century-old university bookstore in Ireland is raising questions about the propriety of lecturers posting class notes on-line, the Bookseller reports. The chairman of a UK academic publishing-industry lobby group claims the practice harms academic booksellers.
The Bookshop at Queens, at the Queen's University Belfast, has been in business for 53 years, but has hit tough times and is closing while it is still above water.
Tim Smyth, manager of the Queen's bookshop, said the bookseller wanted to close before it "fell below the line". He also blamed internet competition and free sites such as Google Scholar and Wikipedia for a decline in custom. He said: "I don't know how [independent booksellers] can do it anymore. Certainly the academic model has reached a tipping point now: it is unsustainable."
With its traditional high prices and low margins, academic publishing has been in trouble for some time now. Students often balk at the prices of textbooks and can resort to practices such as rentals, textbook sharing, photocopying, and, more recently, Internet e-book piracy to stretch their budgets, and that's leaving aside the practice of online note-posting and university course reserves that use copyrighted materials.
Clearly, the academic publishing industry needs to get its business model straightened out. If academic publishers and bookstores fail, what might it mean for the university education process?
Textbook publisher Follett threatens price-comparison browser plug-in
Osprey captured by Bloomsbury
Purdue reveals an alarming future for academia with Amazon?
Morning Roundup: Author rejects award. Women who write horror and more
Amazon wants co-op payments, and also concessions in the UK
It's a shame the bookseller is caught in the middle, but I agree with Thorn: the old model of tertiary textbook supply is an unsustainable oligopoly which extracts super-profits. Many more university students are now paying fees for their courses, and these should cover the materials needed to complete them: they don't deserve to be hit with an additional cost for overpriced texts.


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Alison Dame-Boyle
Alison graduated from Stanford with a degree in English and Philosophy, and is ecstatic to have a job where she can write to do good. Prior to joining EFF, she spent summers working at the Humanities, Arts, Science, and Technology Advanced Collaboratory (HASTAC) and the Center for Adolescent Health and the Law (CAHL), where she helped write and edit State Minor Consent Laws: A Summary, 3rd ed., as well as one summer backpacking around Ecuador with only very rudimentary Spanish. She also did some freelance writing on vintage and DIY fashion in San Francisco. Alison is generally pro- reading, chocolate, traveling, baby animals, hiking, Oxford Commas, and EFF and generally anti- mayonnaise, apathy, and DRM.


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Stauffer likely done for 2012, Padres future uncertain
Hundley, who was optioned to Triple-A Tucson when Grandal originally joined the Padres on June 29, was 0-for-11 with three strikeouts since being recalled on Aug. 9. His batting average on the season has plunged to .157 with 56 strikeouts in a total of 104 at-bats.
Grandal immediately rejoined the starting lineup Friday night. He had played the past two night for Single-A Lake Elsinore, going 0-for-7 and catching one game.
Oblique strains usually take a month to six weeks to heal.
"When I was first injured, we didn't think I'd be able to play again as soon as I am," said Grandal. "I had to beat the odds. The training staff got to the injury right away and I think that was very important. One of the reasons I went on the rehab assignment was to make sure I was 100 percent.
Padres notes
--Starting pitchers Andrew Cashner (latissimus dorsi strain) and Anthony Bass (shoulder inflammation) each threw three innings in a simulated game at the Padres Peoria, Ariz., training base Friday and are scheduled to make rehab starts for Triple-A Tucson next week.
--Right-handed closer Huston Street was still limping in the clubhouse Friday seven days after suffering a strained left calf muscle.
--Third baseman Chase Headley went hitless in back-to-back games Tuesday and Wednesday in Atlanta. It marked the first time since June 24-25 that Headley went hitless in back to-back games and only the third time since he went hitless in three straight games May 11-13.


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Helix Sconces - Set of Two
Item # BBHD1650
♦ Dimensions
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DescriptionOur exclusive Helix sconces, set of two, can be hung as a staggered pair or mounted in multiples along a wall. With geometric precision, the helix pattern is embossed onto a waxed metal sheet, then crafted into a scroll shape. Silver highlights are a final touch to enhance the raised pattern of the Helix sconces.MetalHand finishedEach holds up to 3"" diameter pillar candle, not included
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text:
Nut & Mushroom Cream
31 Jan 2011
|Vegetable oil
|1 Tablespoon
|1 Ounce (25 Gram)
|1 , peeled and sliced
|2 Clove (10 gm), peeled and crushed
|Green pepper
|1 , deseeded and thinly sliced
|Red pepper
|1 , deseeded and thinly sliced
|Baby sweetcorn
|4 Ounce (100 Gram)
|Whole blanched almonds
|8 Ounce (225 Gram)
|8 Ounce, shelled and blanched (225 Gram)
|Unsalted cashew nuts
|4 Ounce (100 Gram)
|Freshly chopped coriander
|2 Tablespoon
|Oyster mushrooms
|4 Ounce (100 Gram, Small Size)
|For sauce
|1 1⁄2 Ounce (40 Gram)
|1 1⁄2 Ounce (40 Gram)
|Dry white wine
|1⁄2 Pint (300 Milliliter)
|1⁄2 Pint (300 Milliliter)
|English mustard
|3 Teaspoon
|Freshly ground black pepper
|To Taste
|Double cream
|1⁄4 Pint (150 Milliliter)
|Coriander sprigs
|4 (Fresh, For Garnish)
|To Taste
Heat the oil and the butter or margarine in a heavy-based frying pan.
Saute the onion and garlic for 2-3 mins.
Add the peppers and the baby sweetcorn, and cook for a further 5 mins.
Add the nuts, coriander and mushrooms, then continue to cook for 5 mins.
Keep warm.
To make the sauce, melt the butter or margarine in a pan, stir in the flour and cook for 1 min.
Gradually blend in the dry white wine and milk to form a smooth thick sauce.
Add the mustard, season to taste and continue to cook, stirring throughout, for 2-3 mins.
Pour the sauce over the nut mixture, then add the cream and stir well.
Transfer to a serving dish, garnish and serve.
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Nut & Mushroom Cream Recipe


text:
Deontay Wilder Becomes 1st American To Win Heavyweight Title Since 2006
Deontay Wilder became the first American to win a piece of the heavyweight title in nearly a decade Saturday night, staggering Bermane Stiverne early on his way to a 12-round unanimous decision that kept him unbeaten in 33 fights.
Nevada Suspends Chavez Jr. After Testing Positive For Marjiuana
The Nevada State Athletic Commission has temporarily suspended Julio Cesar Chavez Jr. after the former middleweight champion failed a drug test. The commission made the standard procedural move Tuesday. Chavez tested positive for marijuana use last month.
Mexican 'Canelo' Alvarez Punishes Josesito Lopez To Defend WBC Title
Saul "Canelo" Alvarez stopped Josesito Lopez late in the fifth round Saturday night, defending his WBC 154-pound title with a relentless display of power by the 22-year-old Mexican champion.


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Electronic program guides in which program listings may be graphically presented and in which sponsors and celebrities of those programs may be identified are provided. The electronic programs guides may be passive television program guides, interactive television program guides, and/or on-line television program guides. Television show text information, logos and/or trademarks, celebrity images, affiliation indicators, and advertisements may be present in the program listings to enhance the electronic program guides.
1. A method of presenting an electronic program guide, comprising:
receiving program guide data; and
presenting the program guide data as program listings for a plurality of television programs, wherein the title or name of at least one of the plurality of television programs is graphically branded to emulate the title or name of the television program as it appears in a broadcast.
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34. A system for presenting an electronic program guide, comprising:
program guide provider equipment that transmits program guide data; and
program guide display equipment that receives the program guide data and presents the program guide data as program listings for a plurality of television programs, wherein the title or name of at least one of the plurality of television programs is graphically branded to emulate the title or name of the television program as it appears in a broadcast.
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This application claims the benefit of U.S. Provisional application No. 60/174,443, filed Jan. 4, 2000, which is hereby incorporated by reference herein in its entirety.
This invention relates to electronic program guides, and more particularly, to promotional techniques for program guides.
Electronic program guides are used to provide program listings information to television viewers. Program listings information may include program titles, scheduled broadcast times and channels, program descriptions, etc.
One form of electronic program guide is the passive television program guide. Passive television program guides, such as the TV Guide Channel, are typically provided by cable system head-ends or other television distribution facilities on a dedicated television channel. Passive program guides may provide program listings in the form of an automatically scrolling or paged list. Viewers may access the listings of the passive program guide by tuning to the appropriate dedicated television channel with a standard television or set-top box.
Another form of electronic program guide is the interactive television program guide. Interactive television program guides, such as TV Guide Interactive and Gemstar Guide Plus, are typically implemented on set-top boxes or other user television equipment with suitable processing circuitry. Users typically interact with such interactive television program guides using a remote control. Features that are often offered by interactive television program guides include the capability to set favorite channels or programs, the capability to set reminders for programs of interest, and the ability to establish parental controls.
Yet another form of electronic program guide is the on-line television program guide. On-line television program guides, such as TV Guide Online, allow users to access program listings information over the Internet using a web browser or similar interface. When using these guides, users frequently may click on program titles within the guides to view additional information such as a detailed program description.
All of these variations of electronic program guides provide program listings information to their viewers or users in the form of text. This can be seen in passive products such as the TV Guide Channel or Americast's passive program guide, both of which feature a text-based scrolling grid of listings. Text-based listings are also seen in Internet guides such as the GIST on-line program guide and the Click TV on-line program guide. Text-based listings are also seen in interactive program guides such as Starsight and Americast interactive program guides.
Nevertheless, there is a need for a more aesthetically pleasing and eye-catching methods for presenting electronic program guides. Preferably these methods would incorporate program listings that are easy to identify and that indicate relationships between sponsors and celebrities of those programs.
It is therefore an object of this invention to provide an electronic program guide that presents program listings as graphical images that can meet these needs.
This and other objects of the invention are accomplished in accordance with the principles of the present invention by providing electronic program guides in which program listings may be graphically presented and in which sponsors and celebrities of those programs may be identified. The electronic programs guide may be passive television program guides, interactive television program guides, and/or on-line television program guides. Television show text information, information icons, graphic logos and/or trademarks, celebrity images, affiliation indicators, and advertisements may be present in the program listings to enhance the electronic program guides.
Further features of the invention, its nature and various advantages will be more apparent from the accompanying drawings and the following detailed description of the preferred embodiments.
Turning first to
When implemented as a passive television program guide, system 100 may be implemented with equipment 120 being a computer and video generator at a cable head-end, link 130 being a one-way cable television distribution system, and equipment 140 being a television. Program guide data then may be formatted by the computer, converted to a video signal by the video generator, transmitted through the cable television distribution system, and then received by and displayed on the television.
When implemented as an interactive television program guide, system 100 may be implemented with equipment 120 being a server at a cable head-end or other location, link 130 being a one-way or two-way cable television distribution system, and equipment 140 being an advanced set-top box and a television. Program guide data may the be transmitted from the server through the cable television distribution system, received by the set-top box, displayed on the television, and interacted with by a user using a remote control of the set-top box.
When implemented as an on-line television program guide, system 100 may be implemented with equipment 120 being a server, link 130 being a computer network that may include the Internet, and equipment 140 being a computer that is capable of communicating with the server in equipment 120. Program guide data may then be formed by the server into a web page, transmitted by the server through the computer network to the computer, displayed on a computer monitor connected to the computer to the user, and interacted with by the user using a mouse and/or keyboard attached to the computer.
Obviously, many variations of suitable equipment may be used to provide an electronic program guide in accordance with the present invention without departing from the spirit and scope of the invention. For example, the present invention may be implemented in a satellite television system.
Turning to
As illustrated in
As shown, information area 206 may include program listings for the electronic program guide. In some embodiments, the program listing may be displayed as buttons 306. Buttons 306 may include text information pertaining to each program, such as the title of the program, a description of the program, names of actors in the program, etc, and informational icons that indicate the rating of the program (e.g., PG, G, TV-13, etc.), whether the program is in stereo, whether the program is closed-captioned, whether the program is in Dolby, whether the program is broadcast in High-Definition Television (HDTV), etc. Buttons 306 may be color coded to indicate what category of show each represents. Each button 306 may be aligned with a channel indicator 308 to indicate what channel a corresponding program is on. Each channel indicator 308 may indicate the channel number, call letters, name, logo, and/or any other suitable indicator of the channel indicator. Each channel indicator 308 may also be in a similar style to guide provider logo 303.
Although the program listings are shown herein as being displayed in buttons 306, program listings may be displayed in any suitable graphical element, such as a grid cell.
An information tray 402 may also be included in information area 206 as shown in FIG. 4. Information tray 402 may contain a legend 404 that indicates the scheme used for color-coding buttons 306, if any. For example, legend 404 may indicate that buttons 306 corresponding to sports programs are indicated in green and that buttons 306 corresponding to movies are indicated in red. Thus, as shown in
Turning to
Like buttons 306 with text content, buttons 306 with graphic content may have colored backgrounds to indicate to the viewer what type of program is showing. For example, "F�R�I�E�N�D�S" button 604 may have a yellow background to indicate that it corresponds to a situation comedy (sitcom) or drama. "SportsCenter" button 606 may be colored green to indicate that it corresponds to sports related programming.
Buttons 306 may also indicate affiliation of programs and/or the program guide with an affiliated entity by providing graphical element affiliation indicators within the buttons. For example, buttons 306 may include one or more affiliation indicators to indicate network affiliation (e.g., ABC, NBC, etc.), sports team affiliation (e.g., Green Bay Packers, Denver Broncos, New York Yankees, etc.), corporate affiliation (e.g., NFL, NASCAR, National Geographic, etc.), event affiliation (e.g., Super Bowl, Daytona 500, Kentucky Derby, etc.), guide affiliation (e.g., TV Guide, etc.), etc. Affiliation indicators may indicate sponsorship of a program by a sponsor, or may indicate any other affiliation between an entity and a program.
Button 306 may also include graphical element celebrity images as shown in FIG. 7. As illustrated, buttons 702, 704, and 706 have images of celebrities in the buttons. Celebrity images on these buttons help viewers identify programs because viewers associate celebrity images with programs. Button 706, for instance, shows the faces of the cast members of the show "Party of Five." As is evident in
Other examples of celebrity images within buttons are shown in FIG. 8. As can be seen, in button 802, a program logo, network affiliation indicator, and a celebrity image are all included. Alternatively, as shown in button 804, only a program logo and a celebrity image may be used. As yet another alternative, a program logo may be positioned within a celebrity image as shown in button 806.
By providing logos, affiliation indicators, and celebrity images in buttons, the program guide of the present invention provides increased readability. Because less time is spent reading the program listings, viewers can focus more time looking at other parts of the screen. This is particularly useful because this provides an incentive for companies to advertise in the program guide and/or sponsor programs indicated in buttons 306. In order to provide more space for advertisements, bigger channel buttons may be implemented in the program guide.
Turning to
As can also be seen in
Buttons 306 may also include promotional slogans to indicate significant events. Slogans may include phrases such as "Final Episode," "Season Finale," or any other suitable phrase.
Turning to
Two other forms of promotion buttons are illustrated in
As shown in
The concepts shown and discussed in conjunction with the figures may be used in passive, interactive, and online guides. The arrangements of screen elements shown are merely illustrative. For example, see U.S. patent application Ser. No. 09/357,941, filed Jul. 16, 1999 and U.S. patent application Ser. No. 09/354,602, filed Jul. 16, 1999, which are hereby incorporated by reference herein in their entirety, for examples of arrangements of interactive program guides, and U.S. patent application Ser. No. 08/938,028, filed Sep. 18, 1997, which is hereby incorporated by reference herein in its entirety, for examples of arrangements of elements in an online program guide. The screens used in accordance with the present invention may or may not be partitioned into various sections containing, for example, program listings, video windows, advertisements, logos and screen titles, etc. Program listings may be in the form of a grid, a list sorted by time, channel, category, alphabetically, or in any other format.
In the interactive embodiments, the guide may allow the user to highlight any individual item and select it using a "Select", "Enter", "Ok", or similar key. In the online embodiments, the guide may allow the user to select any individual time by clicking on it using a mouse. Selectable items may include any button, logo, indicator, text, icon, etc.
Selecting an item may cause the guide to perform an action (such as tuning to a program) related to the item, display additional information related to the item, display a menu of options related to the item (e.g., watch, remind, lock, favorite, order, other air times, etc.), or other function.
The foregoing is merely illustrative of the principles of this invention and various modifications can be made by those skilled in the art without departing from the scope and spirit of the invention.
Patent Citations
Non-Patent Citations
Referenced by
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text:
1878 $5 Five Dollar, Judd-1574, Pollock-1766, High R.6, PR63 Gilt NGC....
2009 January Orlando, FL FUN Auction #1121
|Sold for:
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|Jan 7, 2009
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Orange County Convention Center
1878 Pattern Five Dollar, Judd-1574, PR63 Gilt1878 $5 Five Dollar, Judd-1574, Pollock-1766, High R.6, PR63 Gilt NGC.
Design. The William Barber Flowing Hair motif graces the obverse. Liberty faces left, wearing a headband eponymously inscribed. E PLURIBUS UNUM is above, the date 1878 below. The reverse offers an eagle with lowered wings in the center, with UNITED STATES OF AMERICA and E PLURIBUS UNUM around. The arrows are long and slender, their tips extending behind and past the eagle's wing, and the olive branch has six leaves. Struck in a larger diameter than regular half eagle issues, in copper, gilt, with a reeded edge.
Commentary. The series of 1878 half eagles, Judd-1568A through 1574A, were struck on larger-diameter, thinner planchets in an effort to combat the plague of malefactors scooping out the center of a gold coin and replacing it with less-expensive metal. Mint Director Henry Linderman's 1878 Annual Report states:
"... the worst danger which threatens our gold coin, from counterfeiters, is the filling with an inferior metal or alloy. By this art the piece presents genuine exteriors, but the inner part removed, a disk of platinum, pure or alloyed, is inserted in its place and closed with a ribbed rim of gold. Its is therefore partly genuine and partly counterfeit, and its value reduced by several dollars, differing according to the denomination of the piece.
"The largest chance of spoilation of course occurs with the twenty-dollar pieces, but the pieces of ten and five dollars have also been filled. So far the mischief has been very limited, as it evidently requires first-class workmen, and is slow work; but pieces of this sort are, of all false issues, the most difficult to detect."
Physical Description. Smooth, even gilding coats both sides of this pretty piece. A couple of minor areas of darker toning appear on the reverse in the arrows and the right wing, but there is plenty of appeal for the PR63 grade. NGC Census: NGC has certified six examples in all grades, including gilt and nongilt coins. Of the two gilt pieces at that service, this is the finest. PCGS Population: Six pieces total, none of which is noted as gilt (12/08).
Provenance. Ex: Auction '89 (Stack's, 7/1989), lot 1865.
From The Lemus Collection, Queller Family Collection Part Two. (PCGS# 61937)
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text:
Security Consulting Services
Matryx Consulting can assist with all areas of business security across any industry anywhere in Australia.
We provide security solutions that suit your specific needs and business environment and advise on the best technical and operational systems required to achieve your overall objectives.
Your project could be anything from needing to implement new technologies; upgrading your existing security systems; understanding what your risks are; or to writing an emergency management plan.
Whatever your security requirements are, we can help.
If you are unsure, call us for a no obligation chat and we can point you in the right direction. Sometimes, bouncing ideas around with an expert in the area is all you need.
Below are some of the areas we specialise in:
- Security Consulting
- Security Risk Assessments
- Security Solutions Design
- Security Implementation & Management
- Security Managed Services
- Physical Security Solutions
- Electronic Security
- CCTV Surveillance and Design
- Council SafeCity CCTV Design
- PSIM (Physical Security Information Management)
- Counter-Terrorism Risk Assessments
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Vintage cloth uniformed doll, possibly a Canadian Mountie. Probably made by Norah Wellings.
He is 11 1/2" tall. All original with complete costume including the hat. His boots and garment straps are made of leather.
He is in very good condition. Looking carefully and being very particular, I will point out that there is a minor smudge on his left cheek and his hat has a tiny separation in the front. There are two small holes, one on the back of the neck and one on the back of the hat.
No odors and from a smoke free home.
If you are the pending buyer of this item, go to My Account to view, track and pay for this item.
Treasures worthy of the next generation
Offering investment worthy dolls, antiques & vintage items.


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You can now invite anyone to meet at your favorite Starbucks.
Limited-time feature
27 year old woman | Clarkston, MI, USA | Seeking men 27-35 within 25 miles
Must love dogs
Hey there! Lets start with my flaws....I sleep with way to many pillows on my bed, I sing far too loud in the car and I have way to many shoes in my closet. I am a really outgoing, playful and a love to laugh kind of person. I enjoy having a good time and i truly cherish the people I have in my life. My friends are my family and I hold them dear to my heart. I'm pretty sarcastic and joke around a lot. I love my career I work in healthcare I'm a very driven goal oriented person. My friends would say I am very dependable and independent. I'v lived a lot of real life experiences in my short 25 years. I consider myself pretty laid back.I feel like I'm the kind of girl who is comfortable in heels drinkin a beer with the boys. I love live music, I live right down the road from a major music theater so I spend many summer nights listening to live bands. My cabin up north is one of my favorite places to be, I sneak up there as many weekends as I possibly can. Floating down a river is on my top 5 things to do. My dog Oberon is a big part of my life, we love walks or hikes at the park, so if you are not a dog person there's no reason to even message me it wont work. Obie and I are pretty active. Health is something that is very important to me and I want the person I am interested in to maintain an active life as well. In the winter I try to get out and enjoy the snow, oddly enough I have been skiing my whole life and I am terrible (I'd love some pointers if you have some) I like a low key night in watching a good movie as well, I like to cook. Theres not much I wont try. The guy I'm really looking for is the guy who is going to make me smile and laugh but knows when to be serious. I'm looking for someone who has ambition and goals and goes after them.I want a guy who is down to earth and isn't afraid to get dirty. I love to be challenged and I want to challenge someone. I'm looking for someone who can be comfortable in his own skin and in several different situations, I have diverse groups of friends and it is sexy when a man can hold his own in a room of people. I am pretty traditional, maybe a little old school I think a guy should act like a gentlemen. If this catches your eye shoot me a message I'd love to chat.
Camping , Coffee and conversation , Cooking , Dining out , Movies/Videos , Music and concerts , Exploring new areas , Performing arts , Playing cards , Playing sports , Shopping/Antiques , Travel/Sightseeing , Watching sports , Wine tasting
Bowling, Cycling, Dancing, Other types of exercise, Skiing, Swimming, VolleyBall, Walking / Hiking, Yoga
I exercise 5 or more times per week.
I have Dogs
I have an American Pitbull Terrier who is great!!!
Middle of the Road
My cabin up north, being on a river in a tube or kayak. Pine Knob music theater, Nashville, On any beach.
I love country music, Reality tv is a guilty pleasure, Justified, Boardwalk Empire, Dexter. Movies Goodfellas, Training Day, Dirty Dancing. I enjoy easy reading. I like a good craft beer. Favorite foods Italian, Chinese, Good steak Anything homemade
I enjoy all outdoor activities. Spending time with my dog. The gym, live music, sporting events, Tigers games, trying new things, a good craft beer. Skiing in the winter (even though I'm terrible). I love to laugh so doing anything fun. Wallyball.
The last thing I read was the 50 shades of Gray Trilogy. I enjoy all Nicholas Sparks books.
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What she is looking for
|5'7" (170cms)
|5'8" (172cms) to 6'4" (193cms)
|Body type:
|Slender, About average, Athletic and toned, A few extra pounds, Stocky
|No preference
|No preference
|No Way
|No Way
|Social Drinker
|Social Drinker
|Administrative / Secretarial
|No preference
I work in a unique area of healthcare administration.
|I'll tell you later
|No preference
|Never Married
|No preference
|Have kids:
|Wants kids:
|White / Caucasian
|White / Caucasian
|Christian / Other
|Christian / Catholic
|I'll tell you later
|Some college, Bachelors degree, Graduate degree, PhD / Post Doctoral


text:
LIVINGSTON, NJ -- (Marketwire) -- 11/15/12 -- SilverSun Technologies, Inc. (OTCBB: SSNT) (OTCQB: SSNT), a preferred source for best-of-breed business management applications and professional consulting services, today announced record third quarter financial results for the three and nine months ended September 30, 2012.
"We are very proud of our strong operating performance this quarter, as we delivered results that were at or above our expectations, thereby setting the stage for another record-breaking year for SilverSun," stated Mark Meller, Chairman, President and CEO of SilverSun. "Our notable progress in the first nine months of 2012 can be credited to the diligent efforts of our sales and marketing teams across the country coupled with our success in making several accretive acquisitions during the year. Given that, we have high confidence that SilverSun will perpetuate the positive sales trend we have achieved for the past six consecutive quarters; and we fully expect to end 2012 on a high note and embark on 2013 on very strong footing."
Financial Highlights for the Three Months Ended September 30, 2012 Compared to the Three Months Ended September 30, 2011:
Financial Highlights for the Nine Months Ended September 30, 2012 Compared to the Nine Months Ended September 30, 2011:
As of September 30, 2012, the Company had $101,340 in cash; $1,667,757 in accounts receivable; zero long term debt; and total stockholders' deficit of $570,800.
For more details on SilverSun's third quarter results, please refer to the Company's 10-Q filed yesterday afternoon with the U.S. Securities Exchange Commission and accessible at www.sec.gov.
About SilverSun Technologies, Inc.
Headquartered in Livingston, New Jersey, SilverSun Technologies is involved in the acquisition and build-out of technology and software companies engaged in providing best of breed management applications and professional consulting services to small and medium size businesses (SMBs) in the manufacturing, distribution and service industries. Serving as SilverSun's principal operating subsidiary, SWK Technologies, Inc. employs a national direct sales and consulting team currently serving a growing customer base spanning 38 U.S. states and Canada. For more information, please visit www.silversuntech.com, www.swktech.com or www.mapadoc.com.
Forward-Looking Statements
This press release may contain forward-looking statements, including information about management's view of SilverSun Technologies' future expectations, plans and prospects. In particular, when used in the preceding discussion, the words "believes," "expects," "intends," "plans," "anticipates," or "may," and similar conditional expressions are intended to identify forward-looking statements. Any statements made in this news release other than those of historical fact, about an action, event or development, are forward-looking statements. These statements involve known and unknown risks, uncertainties and other factors, which may cause the results of SilverSun Technologies, its subsidiaries and concepts to be materially different than those expressed or implied in such statements. Unknown or unpredictable factors also could have material adverse effects on SilverSun Technologies' future results. The forward-looking statements included in this press release are made only as of the date hereof. SilverSun Technologies cannot guarantee future results, levels of activity, performance or achievements. Accordingly, you should not place undue reliance on these forward-looking statements. Finally, SilverSun Technologies undertakes no obligation to update these statements after the date of this release, except as required by law, and also takes no obligation to update or correct information prepared by third parties that are not paid for by SilverSun Technologies.
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Dodi Handy or Kathy Addison
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text:
Teachers may be masters of the classroom, but some are still struggling to learn the ABCs of social networking, where the line between the professional and the personal blurs all too easily.
As Facebook, Twitter and others have grown in popularity, more and more U.S. teachers have reportedly been disciplined -- even fired -- for sharing photos and messages deemed inappropriate by their school systems.
So some school districts are trying to get a leg up on social media in schools, drawing up policies that outline what teachers can say and whether they can "friend" their students online. But the policies aren't without critics, who say school restrictions on social media may infringe on teachers' speech rights in the coming years.
A Massachusetts teacher was asked in August to resign after posting comments on her Facebook wall describing students as "germ bags" and parents as "snobby" and "arrogant." Although the teacher said she intended the comments for her close friends only, her privacy settings were open enough that others in her town could see what she had to say.
In Manatee, Fla., a teacher was recently suspended without pay for five days after writing on Facebook that he hated his job and his students.
As a result, all teachers in Manatee are on the verge of facing new rules regarding what they can and cannot say on social media sites.
"That teacher made a post that said, 'I hate my kids, I hate my job, I don't want to go to work.' … That's something that's going to affect your ability to do your job," said John Bowen, an attorney for the Manatee School District.
"If you wouldn't say it in the classroom, use common sense. Don't use it in some other media that may get out to students and parents. It may be you can say those things privately all you want, but if it gets out to the wrong people or students and parents, then it becomes our business."
The school board will vote within the next month on a policy that hems in teacher behavior online, he said. Teachers are allowed to "friend" students on Facebook but not to communicate with them online without notifying parents first.
The new policy also limits what they can say on Facebook and comments that cast the school or students in a negative light are out of bounds, he said.
Bowen said Facebook can be a valuable tool and officials don't want to restrict access altogether but want teachers to know that if they "extend the classroom to cyberspace, the rules go with you."
If a teacher violates the rules, he or she could be subject to a letter of reprimand, a suspension or an outright dismissal if the actions are egregious enough, he said.
School administrators in Cedar Rapids, Iowa, for instance, are considering a similar proposal that would ban teachers from "friending" or "following" students on social media sites and limit online communication to school-related correspondence only.
"We were not doing this as a reaction to any specific incident," said Richard Whitehead, superintendent of the College Community School District in Cedar Rapids. "We're simply doing it because social networking is growing so rapidly … and we just think our teachers, in order to protect themselves and students, need to use it in a common sense kind of way."
Teachers in his district are encouraged to use Facebook but also urged to use it wisely.


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I was reading on one of the bookmarks at the Bellevue Library about the facts about tobacco. I was shocked to find out cigarettes contain ammonia and arsenic. How is it possible that they can sell to the public something that contains ammonia and arsenic? Doesn't that constitute endangering the public? When there are recalls about other food products that can endanger your health, they have to pull it off the shelf. I went on the cigarettes Web site and found out there are 599 chemicals added to cigarettes. When they are burned it created more than 4,000 chemical compounds.
The fact sheet on the bookmark also says more people die from smoking related illnesses than from AIDS, car accidents, illegal drugs, murders and suicides combines. One third of all new smokers will eventually died from tobacco use. That nicotine is more addictive than cocaine and heroin. That 53,000 people every year die from second-hand smoke. These are facts that you heard before. How about you lose 11 minutes of life for every cigarette you smoke and an average of 14 years off your life span. Is it worth it for a puff of smoke?
If they are as addictive as cocaine and heroin and can make you sick and cause death, how can they keep on producing them? How about the FDA banning these chemicals that are so addictive and dangerous. How about making them pay for all the health problems they are causing instead of ads about not smoking. Let them pay into the health care system and build health care clinics.
Ruth Dendinger


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About Us
East Greenwich Spine & Sport is a multidisciplinary clinic specializing in the treatment and diagnosis of conditions of the nervous and musculoskeletal systems. Offering consultations and independent medical evaluations, we work together with our patients to develop personalized rehabilitation plans. We were founded in 2003.
News at EGSS
East Greenwich Spine & Sport and Affinity Sport Medicine become one care delivery team under Affinity Physicians. See letter
Oct 2014
Dr. Pasquarello is appointed to the Accreditation Council for Graduate Medical Education's (ACGME) newly created Osteopathic Principles Committee that will develop osteopathic standards for intern, resident and fellowship training programs designated as "Osteopathic Focussed" under the new single accreditation system for American Osteopathic Association (AOA) and ACGME postgraduate training programs.
Sep 2014
Dr. Pasquarello, represented the osteopathic medical profession at a Congressional briefing, "Back Pain: America's $50 Billion Challenge." Presented by the Arthritis Foundation, the briefing educated Congressional staff and stakeholders on the prevalence and costs of chronic back pain as well as strategies for improving the diagnosis and care of patients with back pain.
Aug 2014
Dr. Smith begins the Executive Master of Healthcare Leadership program at Brown University
Jul 2014
Dr. Pasquarello serves as the Rhode Island Delegate at the American Osteopathic Association House of Delegates Meeting in Chicago, IL
Jul 2014
East Greenwich Spine & Sport joins Affinity Physicians, a service of Care New England


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Argues that a Comparative Analysis of Global Credit Card Data Shows a Link to Increasing Bankruptcy Filings, Recommends Major Policy Reforms
AUSTIN, Texas — Professor Ronald Mann of The University of Texas School of Law will offer a broad-ranging critique of credit card policy when he delivers the Akard Lecture at the Law School on Wednesday, Nov.19, the evening before UT Law's Annual Bankruptcy Conference. The lecture, which is free and open to the public, begins at 5 p.m. in the Connally Center's Eidman Courtroom with a reception to follow.
Mann's speech, entitled "Policy Implications of the Global Growth of Card-Based Payment Systems," will focus on the findings of his multi-country study exploring the implications of the rapid growth in global credit card use. Mann, a 1985 UT Law graduate and the William Stamps Farish Professor in Law, is widely recognized as one of the leading commercial law scholars of his generation and is the most frequently cited legal scholar working in any of the business law fields to have entered law teaching in the last decade.
The most provocative
parts of Mann's work relate to the significance of credit card use in the
many countries in which they are common. As an empirical matter, he presents
the first multi-country analysis of the relation between credit cards and
bankruptcy. Relying on data about bankruptcy filings and borrowing from
Mann's analysis
of that point shows that even if a country's overall level of consumer debt
does not change, a shift in borrowing patterns that reflects increasing use
of credit cards will result in an increase in the total amount of consumer
bankruptcy filings. Mann explains: "What the data suggest is that credit
cards are uniquely associated with a particularly prodigal sort of borrowing.
The gist of it is that borrowing on credit cards is too easy, so easy
that borrowers fail to appreciate the risks they would see if they borrowed
the money in a more traditional way." These findings raise important policy
questions, coming as they do days after announcement of official 2003 statistics
showing another major increase in consumer bankruptcy filings in the
Mann's lecture
will also address a variety of potential policy initiatives that could be
brought to bear on the problem. "The use of credit cards differs dramatically
from country to country. Few use credit cards as much as Americans, and nobody
uses them to borrow as much as Americans. But regulators in Europe and Australia
are taking stern steps to limit rising credit-card use in their countries,
even though it is much less common than it is here," said Mann. "Unfortunately,
most of those regulatory initiatives are fundamentally misguided, because
they address the wrong problem." For example, he points to Japanese regulations
that for decades limited credit-card use by barring banks from issuing credit
cards. The problem with those initiatives is that they have not discouraged
debt more broadly. The end result has been a shift of consumer borrowing
to lenders loosely affiliated with organized crime. With respect to
Eschewing traditional regulatory initiatives, Mann argues instead that regulators should consider novel ideas, such as banning affinity programs. "Almost every time that an American cardholder decides whether to use a credit card as opposed to a debit card or a check or cash, the decision is influenced by an affinity program that provides a substantial tangible incentive in favor of the credit card transaction. If we removed that incentive and let cardholders make those decisions based on the merits of the different payment products, we might see a substantially lower volume of credit card borrowing."
He also argues that the current credit card disclosure regime is fundamentally misguided. By focusing on paper disclosures delivered at the time of the application rather than information delivered at the point of borrowing, the present system expends a great deal of resources to provide no useful information. A system that advised cardholders at the time of the transaction of their current credit level and of any fees associated with the transaction might produce much more intelligent decisions than the existing regime.
The speech builds on Mann's ground-breaking work in electronic commerce and payment systems. Mann is the author of Electronic Commerce (with Jane Winn), Payment Systems, and numerous law review articles related to payment systems and commercial finance. He is a member of the American Law Institute and currently serves as the reporter for amendments to Articles 3 and 4 of the Uniform Commercial Code.
"Ronald's research explores the impact of different international attitudes towards consumer credit. As a result, we will be better able to understand what drives that market and the social and economic effects of credit card use as opposed to other forms of consumer credit," said international insolvency expert and award-winning UT Law professor Jay Westbrook.
Mann received
his J.D. from The University of Texas School of Law, where he graduated first
in his class and was managing editor of the Texas Law Review. After clerking
on the U.S. Court of Appeals for the Ninth Circuit, he clerked for Justice
Lewis F. Powell of the U.S. Supreme Court. He served as an Assistant to the
Solicitor General of the
Mann's multinational study in the variation of credit card usage and practice is funded in significant part by the Marlow R. Preston Faculty Fellowship in Bankruptcy Law. The John C. Akard Distinguished Lectureship Program was endowed by the generous gifts of many members of the Texas bankruptcy bar in honor of Judge John C. Akard, a 1957 UT Law graduate who served with great distinction for 14 years as the U.S. Bankruptcy Judge in the Northern District of Texas, sitting in Lubbock and throughout much of West Texas. The first Akard Lecture was delivered in 2001 by Professor Elizabeth Warren of Harvard Law School, and formerly of UT Law. The 22nd Annual Bankruptcy Conference will be held at the Four Seasons on Nov. 20 and 21 and is offered by The Office of Continuing Legal Education (CLE) at The University of Texas School of Law (www.utcle.org).
Public parking near the Law School is available in the San Jacinto Parking Garage 1 (PG1) with entries on San Jacinto and Trinity streets. For a map of parking at the Law School go to:http://www.utexas.edu/law/about/parking.html . For maps of The University of Texas at Austin go to:www.utexas.edu/maps.


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Viewing comments for browner« back to profile
Rory Arnold banned for bite in process of scoring first ever Currie Cup try
I cant believe what im reading ----- You're a prized prat........... unintentional biting....!!!!! god help us, whatever will be dreamed up next.... i played all my life and ive never seen unintentional biting, its thuggery. Cameras are weeding out the punchers, and the gouger's so the dirty bastards have started biting.... i vote he has his teeth removed as a punishment.
1 Year, 9 Months ago
New #BeattheBoks campaign video causes a stir in South Africa
Fox should hang their heads in shame......... i expect much more from them......... much more piss takin, this is tame. Watch out for horwills feet u boks!!!!!
1 Year, 9 Months ago
Courtney Lawes huge hit in pre-season game vs Bedford Blues
All players have different skills, and impacts, some do the tight work [eg..O'Connell] other score tries ... Croft, Lawes major value to the team is his ability to wreck opposition backs with marginally late albeit fully committed tackles. When will 10's learn ....... When Saints wanted to disrupt Tigers who did they assign to the job? Yep, Lawes.... there's a reason, it's because he's good at it..... The same way Corbisiero has a different value to a team than Vunipola .... A brave but nevertheless daft 10 IMO pass slightly earlier & get Lawes YC for a late hit [cos he's too daft to pull out] , do it twice & he's off the field
1 Year, 10 Months ago
Sharni Williams dubbed Woman of Steel after brutal double knock
I'm not sure whether to congratulate or commiserate you on your dating choices.
1 Year, 11 Months ago
Rene Ranger shows the finger after high tackle by Bundee Aki
It's rather confusing, the players have separated & the direction that he is looking at the time of the finger isn't toward his opponents ...... so who is it at , AR on near side?, his own bench?, own players? fans? oppo bench? this clip is inconclusive - maybe someone could ask him for us !
1 Year, 11 Months ago
Plays of the Week - The Lions Series Awards
Davies was at fault for allowing AACooper to score, JD was looking inward & allowed AAC to get outside him, clearly Man4Man defending needed.
Just saying !
1 Year, 11 Months ago
Wilhelm Steenkamp suspended for two weeks for head charge on Butch James
What does James expect, everyone knows it's tough in amongst the forwards at rucks & mauls .............. keep safe = stay out :)
1 Year, 11 Months ago
Wilhelm Steenkamp suspended for two weeks for head charge on Butch James
I think the only person that comes out of this with credit is Japhta.
Personally I always enjoy seeing tiny ref's talking to huge players, it emphasises why the referee needs to be 100% supported by authorities to discourage all intimidation of officials.
Although seeing Butch James on the receiving end is kinda poetic !
1 Year, 11 Months ago
Butch James suspended for four weeks for high tackle on Jurgen Visser
Law 10.e applies here
Sanction: Penalty Kick
In this case there wasn't an injured player, and the referee was playing advantage [which was duly taken] IMO the post match ban is unwarranted. The referee assessed the situation & could of cautioned or YC or RC , he chose neither - so that should be the end of it.
1 Year, 11 Months ago
George Smith knocked out by Richard Hibbard on Wallaby return
What are these "Tests" ............. the Sports Governors are sending the wrong message ............ heed this http://www.canada.com/sports/School+board+launches+review+after+rugby+player+dies+after+head+injury/8376953/story.html
1 Year, 11 Months ago


text:
Originally written on Fox Sports Southwest | Last updated 12/6/12
SAN ANTONIO On Wednesday night, in New Orleans, NBA commissioner David Stern made sure to be on hand when one of the sexiest, glitziest teams in the league adorned fans and media with something equally shiny and impressive: Kobe Bryant becoming just the fifth person in history to score 30,000 points in his career. But it was 500 miles due west, away from the records and the commissioner and a 9-10 Lakers team that has commanded so much attention, that the San Antonio Spurs continued to be the slow, steady, sure organization that racks up wins in its no-flair style. The kind of fireworks, drama and Q-rating scores Stern has masterfully used to lift the NBA higher in the American imagination? None here, not this team. There are no Big Threes, no ego battles, no stars demanding exits, no Linsanity or teams hijacked by their own players or big-city angst over a so-so season. Just a Spurs squad quietly taking care of the Milwaukee Bucks, 110-99, to lift its record to a near-league bes...
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Teiz Motorsports introduces first ever Women's motorcycle suit
View Single Post
Beastly Adventurer
Joined: Feb 2009
Location: Plano, TX
Oddometer: 1,739
Originally Posted by
I'm going to be the devil's advocate....
I see where you are coming from but....
The comparison is not fair. You are comparing a 2 piece arrangement (not
meant to be worn over jeans) to a 1 piece suit (designed to be an oversuit). I don't think we want to debate which style is better on this particular thread.
It is also a matter of preference. Due to the nature of my business, i am continuously trying out gear from every brand. On even a mildly hot day, i want to rip off all the gear when i take a break - regardless of brand, materials, etc... When you are not moving, any sort of motorcycle gear will get hot (at least for me). Thats why i prefer to have a presentable layer underneath so i can sit and eat in comfort.... A one piece gives me that flexibility... When riding in a two piece suit, "I" always miss the ability to shed the top most layer.. Personal preference.
I am not quite sure how can sitting in (relatively) heavy and hot riding pants be more comfortable than sitting in shorts or comfy jeans. What if you are somewhere in the rural parts of Mexico and eating from a street vendor.. sitting on he sidewalk. 100+ degrees of blazing sun.. how can you not want to rip off those riding pants... My wife & I have been through similar situations elsewhere and she was quite envious of my ability to emerge in shorts every time we took a break.. She got back home and started designing the Revolution suit...
Update: on another forum, a rider expressed concern over how it would be inconvenient to take off the boots, in order to take off the suit. We agree. thats why the suit is designed to be put on and taken off with the boots on.
Teiz Motorsports
#1 Rated Riding Gear
a Revolution for the ladies :)
Get a custom suit - made to your measurements
Follow us on Facebook for awesome pics
| 888-808-8349
teizms screwed with this post 05-24-2011 at
Reason: updated for additional comment about boots
View Public Profile
Visit teizms's homepage!
Find More Posts by teizms


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|Instructor Info:
|Amy Jordan
Office Extension x5644
|Meeting Info:
Struggles for equity in education have always been central to African-American strategies for advancement. African-American ideas about how to make educational equity a reality, however, have varied greatly over time. This course seeks to examine how various issues in African-American education have evolved throughout the twentieth Century. The class will begin with the dynamic struggle of Boston's African American community to desegregate public education during the pre-civil war decade. We will cover other critical campaigns in the Reconstruction, Jim Crow and Civil Rights/Black Power eras.
By exploring a range of critical perspectives on black educational history, students will begin to identify specific research questions. This course will require students to become familiar with resource materials found in the library research databases and in the W.E. B. Dubois Special Collection located at UMASS. You will also have several opportunities to develop your abilities to analyze primary documents in education during classroom discussions. Reading materials will cover a wide range of areas of education, such as school building on the local level, desegregation, competing educational philosophies, Black Colleges and Universities, school boycotts, Black teachers and Civil Rights Movement and early childhood education. You will notice many gaps in the existing literature. Much of the second half of the course will be devoted to exploring new areas of research for a final paper.
Class participation is critical to the success of this course. You are required to complete the readings for each meeting and to develop three thematic questions for class discussions. These weekly assignments require that you take some time to jot down questions as you read. Developing your own critical questions will also allow you to gradually craft research questions that will hold your interest for the duration of the project. Another important aspect of your classroom participation involves your engagement of the recommended readings. You will be required to present one of the recommended readings to the class. In preparation for the presentation, you will write a three page reading response that explores how the recommended reading informs your understanding of the topic for that week.
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The staff and board of ScienceWorks believe that curiosity is the root of a thriving, innovative community. We are committed to sparking curiosity in people of all ages and all culturesserving adults, seniors, children, families, couples and businesses through the fun of creative innovation and invention.
With your help we can continue to build a love of curiosity in schools, homes and communities. Donating to ScienceWorks is a great opportunity to be affiliated with a commitment to excellence in innovation, science and education on a local and national level, while at the same time providing critical funding for important ScienceWorks programs.
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