text:
Lily Allen Tweets About "Leaking" Breast Milk … Again
This must be official boob day. There are Katy Perry boob rumors flying around, Jennifer Lawrence talked about her boobs again and, not to be outdone, singer Lily Allen is tweeting about her breast milk.
Lily recently gave birth to her second daughter, Marnie Rose, and just tweeted to her 4 million followers:
Left house without me nipple pads and me tits are leaking. #coldandwet #notLOL— lilyrosecooper (@lilyrosecooper) February 19, 2013
This is not the kind of information she's shy about sharing.
When Lily (who also has a third nipple and doesn't mind talking about that, either) was nursing her other daughter, Ethel, now 15 months, she tweeted that she could shoot her milk "10ft" from her nipples. She wrote, in response to another tweet, "My let down reflex ... is pretty good, 10 ft sounds about right." She also tweeted about leaking at a London restaurant, writing, "I feel sick and I left the house without breast pads and started leaking all over myself at the #Wolsley .oh and I wiped out in the street earlier #glam (sic)." When a follower complained about her "leaking" story, she responded, "What do you mean it's BEAUTIFUL !!!!! Boobs are not just #sexyfunbags , they be feeding implements too. (sic)"
Let no one say we have not been kept fully abreast of her mammary powers!
Sources: @lilyrosecooper, Monsters and Critics, Contactmusic


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You can now invite anyone to meet at your favorite Starbucks.
Limited-time feature
50 year old man | Beverly Hills, CA, USA | Seeking women 34-43 within 50 miles of Sherman Oaks, CA
Active within 3 days
Looking for that special someone to spoil and share my life.
WHO AM I: I am warm, energetic and enjoy the little things and finer things in life. I enjoy traveling to the far edges of the earth to explore the unknown and at the same time enjoy the responsibilities of my profession and being a parent. Being free to express one's self and being open minded to new ideas helps me to grow as an individual and maintain an understanding of the environment around me. I am intimate, passionate and enjoy bringing happiness to the ones that I love. I enjoy the company of others and hearing of their experiences and ideas. I have a great sense of humor and like to see it those around me. I am loyal, honest, focused, motivated, and tolerant. MY FUTURE PARTNER WILL BE intelligent, sensual, and feminine. She will be sexy, down to earth, tolerant and kind. She'll be confident to discuss her ideas with me and let me know what is on her mind. She will enjoy herself and those around her. With me, she will be passionate, intimate, caring, loyal, and inpiring. She will not worry about the small things in life, but focus on its overall picture. She will want to achieve a better life and ensure that I am part of her future. She will also be my best friend.
Dining out , Fishing/Hunting , Movies/Videos , Music and concerts , Nightclubs/Dancing , Playing cards , Travel/Sightseeing
Auto racing / Motorcross, Billiards / Pool, Cycling, Dancing, Football, Golf, Inline skating, Skiing, Swimming, VolleyBall, Walking / Hiking, Weights / Machines
I exercise 3-4 times per week.
Middle of the Road
My favorite places in town are the Gardens at the Four Seasons Hotel and The Palms resta. My favorite destinations are Paris and Tahiti. I look forward to bringing my new mate to all of these locations and more!
I enjoy the outdoors and almost every activity that one can find in it. I think I have tried (at least once) every sport presently known. At present, I snow ski, water ski, scuba dive, play golf, ride horses, and hike in the local mountains.
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What he is looking for
|6'0" (182cms)
|5'4" (162cms) to 5'10" (177cms)
|Body type:
|Athletic and toned
|Slender, Athletic and toned
|No preference
|Dark brown
|No preference
|No Way
|No Way
|Social Drinker
|No preference
|No preference
I am an attorney at a firm on the West Side. My specialty is product liability defense.
|I'll tell you later
|No preference
|No preference
|Have kids:
|Yes, and they live at home (1)
|No preference
|Wants kids:
|No, but it's OK if my partner has kids
|No preference
|White / Caucasian
|Asian, Middle Eastern, White / Caucasian
|I'll tell you later
|No preference
|PhD / Post Doctoral
|Bachelors degree

subset: commoncrawl


text:
Cole Slaw
6 Nov 2009
This Cole Slaw is a unique and winning salad that is sure to surprise and please your guests with its ingredients and amazing flavor! Trust me they will love this unique Cole Slaw preparation. Try it!
|6 Cup (96 tbs), finely shredded
|Whole-wheat flour
|2 Tablespoon
|Sea salt
|1⁄2 Tablespoon
|1⁄4 Tablespoon
|Caraway seeds
|1⁄2 Tablespoon
|2 Tablespoon
|Cold water
|1⁄2 Cup (8 tbs)
|Egg yolks
|2 , beaten
|Cider vinegar
|1⁄4 Cup (4 tbs)
|1 Tablespoon
|1⁄4 Cup (4 tbs)
Combine flour, salt, paprika, caraway seeds and honey in a saucepan.
Stir in cold water, beaten egg yolks, and vinegar.
Cook over low heat or in a double boiler, stirring constantly until smooth and thick.
Remove from heat and stir in the butter.
Let cool, then blend in the yogurt.
Measure cabbage into a large salad bowl and pour on the dressing, tossing lightly.
Vary this basic cole slaw by adding chopped nuts, raisins, shredded carrots or apples, chopped celery, and alfalfa sprouts.
Use green or red cabbage or combine the two.
Rate It
0 Comments
Cole Slaw Recipe


text:
You know how some people have perfect pitch - they can name any musical note instantly? I don't have perfect pitch and that's something I'm thankful for. I can see the usefulness of it, but I can also imagine significant drawbacks.
I like to listen to music and, free from perfect pitch, I don't have to worry about musical theory, what key the music is in, and whether the musicians are tuned to concert pitch or military band pitch. I can imagine that a listener with perfect pitch hears more of the problems than the wondrous sonorities on offer.
My point is however that there is no way I can ever understand what it's like to have perfect pitch. I can never know how music really does sound to such a person. And they can't understand how music sounds to me either. If they listen to me play, they might realize that I've tuned my instrument a quarter-tone sharp, where I wouldn't know without a reference. But they might not hear the interactions between harmonies the way I do, because I'm not aware of the exact pitches of the individual notes.
But it struck me recently that it might be possible for someone to have such a thing as 'perfect EQ'.
The classic scenario would be where a certain instrument in the mix seems to need a mid-band EQ cut, but most recordists would have to sweep the frequency control to find the right frequency, then fine-tune the gain and Q. Granted, the more experience you have, the quicker this process becomes, and in a sense it can become almost automatic. But I suspect it is a rare person who can listen to a sound and know exactly what EQ it needs, then set that EQ without the need for further adjustment.
But are there such people? Do people exist who can sense the spectrum of an audio signal directly and without thinking about it, like people with perfect pitch sense notes?
That's one question, but there's another...
Suppose that an engineer doesn't have 'perfect EQ', and some people do. What do those people think of that engineer's work? Does it sound like a jumble of frequencies to them?
By the way, I can tell you that perfect pitch isn't always what it's cracked up to be. I once knew an amateur cello player who had an acute sense of perfect pitch. His playing was always out of tune though. Somehow there's a distinction between what you want to hear and what you actually do hear!
P.S. I am totally convinced that success in recording is not down to the quality of your hearing but the degree of attention you pay to what you are listening to. So not having perfect pitch, or 'perfect EQ', is nothing to worry about!
Earlier discussion on this topic...
am stil finding it difficult workin a good eq for my mixed down.am using nuendo 3 in my baby studio. I nid an explainations 2 gettin a prefreable eq for my mix down.am new 2 mixing. And if there are good pluggings workin a better mixdown
Thursday June 23, 2011
I think perfect EQ is a theoretical probability, but a non existent one in real life. Yes, when I mix live, and the band plays in a certain key, I will closely match my EQ settings to the best harmonic values. But can anyone here tell 433,2 from 433,3 Hz? You can maybe tell that it sounds different.
I do a lot of live sessions with some truly great musicians and I seem to proactively get it right in any situation or style, without any soundcheck whatsoever. But for the most part it comes from an innate
musical sense, coupled with experience and knowledge. Musical recognition comes from breathing along, feeling and memorizing. I would agree with Ben Chandler for the most part. And yes, proper board settings make it like a fine tuned instrument.
I would not want EQ perfect in any case, the spur of the moment is where it happens for me... A bit of a non-issue really...
Tuesday June 07, 2011
I wan b a part of winning team. For all over the world in the field of recording
Tuesday June 07, 2011
SANUSI AHAMADU AKA SHANANA an upcoming artist ,the new genaration musicians in west AFRICA ar workin very hard to create a music brand that can have a universal appeal.In Nigeria alone we have so many raw rhethems, that u will not know what do.Ghana , cameron Ivory coast ,all have there unique rhethems. soo much style , even in lagos state where i reside each area has its style of music.ajegunle music. mushin musicfuji FESTAC music .Bariga style.Agege style.For me its all about the mood.what is the mood those who listen to the music, u should lern to read u audience and analyse ,
Tuesday June 07, 2011
I agree! "Perfect EQ" seems to be a bit of a misnomer because the way any engineer will choose to EQ will largely depend upon how they like to mix and the types of EQ's they choose to use. For example, how "perfect" of you sound could you get if you chose to use a stock ITB EQ like Logic Pro's Channel EQ, a third-party plug-in like Voxengo Overtone, or a hardware EQ like the Massive Passive? (Which would be better? I would say that it always depends upon what you're trying to accomplish mix-wise, and furthermore, even if you dialed in similar settings on all three EQ's, you'd obviously end up getting tonally different-sounding results). In addition to what Ben said about "being able to hear the interrelationship of all the elements in a mix", I think a more accurate term would be "Relatively perfect (or adequate) EQ" because any EQ'ing you do to a particular element has to fit the overall context of a mix, even if the element doesn't sound "perfect" all by itself with the way you EQ'ed it. =D
Monday May 30, 2011
Hi. Interesting comments. Perfect EQ - true or not? I have worked with an engineer at a couple of folk/world music festivals and corperate events where many entertainers/bands etc would perform for 30 mins and then a 5 minute off and next band on etc.
This engineer could pick his gain and eq in advance by looking at the performer/ band members/instruments. NO sound check - they just play and within 30 seconds his mixes were sounding very coherent and their monitors were acceptable. Just very minor adjustments after the initial 30 seconds. It was unnerving how he just knew.
So, I think experience can lead to intuitive eq choices. Re pitch, I have spent way too much time with autotune trying to correct a note or two that were grating only to discover they were 1/4 of a note out. Others were also out but they did not stand out. I am not sure why, but some things just are :)
Thursday May 12, 2011
Just become a live sound engineer, you will have to train yourself to be able to hear feedback frequencies and pick out that particular frequency on a 31 band EQ. We can hear feedback and have it gone within seconds during a show by knowing exactly what frequency we're hearing. This has helped me in the studio when mixing by making it easier to know a good starting point for the frequency and q width when I want to make adjustments.
Wednesday May 11, 2011
OK a far as perfect EQ I think it is an over simplification of all the elements that go in to making music. Not only are there environmental factors but there is also the way we perceive music witch has a lot to do with culture and education. In conclusion I think nothing beets experience!
Wednesday May 11, 2011
While I do agree that some people may appear to have perfect EQ, the question is perfect in relation to what? We have gotten used to the model of what certain instruments are supposed to sound like, and it is true that certain instruments live within specific frequency ranges. Some people may have enough experience mixing to appear to have perfect EQ, or they may have memorized a set of paramaters that always work for them. True perfect EQ would seem to live in the savant range. But in the case of the savant, would this knowledge be useful information? I do believe that some people just have a knack for finding the right tone and balance. Some people can have a natural ability to play a musical instrument, while others struggle for years and never "play music". But those with natural ability still have to work at it to become excellent. I think that it is the same in the audio recording domain. The rest of us just have to sweep/boost/dip to find it. And power to the engineer that is looking to break boundries and maybe EQ that snare drum or that vocal just a little (or a whole lot)different than the norm.
Tuesday May 10, 2011
I have a problem trying to suggest that perfect EQ exists, unless it means that the better EQ setting for a song was realized. Unlike perfect pitch which has less to do with the quality of the instrument, amp, speakers, plug-ins, the room used, EQ would seem to be subject to all of that and more. It would be like saying someone has perfect color vision. How is that possible when color is arbitrary with millions of shades of the primary colors of the color spectrum? Searching around within a song for the "right" EQ must have many factors involved; the other instruments in the song and all the EQ settings those are under, the playback system, quality of plugins, the acoustics in the room. If getting a phantom image is subject to proper acoustics then getting "perfect EQ" should fall under the same set of circumstances. And yet this may be subjective as the next generation of engineers, equipment and listeners will have different ears altogether, But A-440 will always be A-440 to infinity.
Monday May 09, 2011
I don't think of myself as having "perfect EQ", but I do seem to hear frequencies in a tonal kind of way. Things out of tune bother me, but not as bad as frequencies that seem to muddle up a mix. Instinctively I can go to an equalizer and get pretty close on my first attempt at clearing things up. Perfect Eq? Never would have thought of it in that way. Rather, a good ear for clearing up a jumbled mess of a mix. I am not sure if there is really "perfect anything". Good at hearing pitch? Good at hearing frequencies? Maybe more along those lines??
Monday May 09, 2011
When I was studying classical guitar performance in college, I had a very few professors with perfect pitch. It was lovely for being able to know what note a chair squeaked at, but didn't really help them play better. The performance was a skill that required work, and they still have to "hear" the quality and musicality of the perfomance like everyone else. An engineer is in the same boat I think. You must hear how it all works together, and sometimes disonance is better than harmony if discord is what works for a particular peice. And honestly, most people don't hear the way someone with exceptional pitch does, so if you are producing a recording being one of the masses has it's advantage since you'lll hear it the way they do too. I hear people talk about this mystical "perfect" element that we all fear we are missing. I think the only element that can bring you closer to "your perfect" production is experience and a constant jouney to do it one step better each time.
Monday May 09, 2011
As an audio engineer, and a professional I've had to train myself to be able to hear frequency ranges, which I think is more in the realm of reality than what "perfect EQ" suggests. Perfect EQ, in my estimation, might be as distracting to engineers and mixers, and as big a curse, as perfect pitch is to those musicians who posses it. We as mixers, recordists and mastering engineers, benefit more from an overall perspective, or being able to hear the interrelationship of all elements of a mix. Boy would I hate to get hung up on one aspect of a mix to the detriment of the balance. Of course, in my case, having been a working musician, for a number of years, never hurt.
Monday May 09, 2011
Learn how Audio Masterclass can help you become a better producer
and engineer in your own home recording studio!


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- Wednesday, May 21, 2014, 7-8:30pm. Kirkland
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Originally written on Fox Sports Florida | Last updated 5/4/12
After Thursday's practice session, Orlando Magic head coach Stan Van Gundy said he was considering lineup changes for Saturdays Game 4 against the Indiana Pacers. No wonder. The coach is do doubt looking for answers as he looks at numbers that tell a striking story. In the first three games of the best-of-seven series, the Pacers starting five have outplayed the Magic starters. Badly. Heres how the numbers look: Indianas starting five, on the court together, have an offensive efficiency rating of 121.2, meaning, the Pacers are scoring an incredible 121 points per 100 possessions. Pacer starters have a defensive efficiency rating of 83.9, which means they are giving up just 84 points per 100 possessions against the Magic. Subtract the defensive rating from the offensive rating, and you have a net efficiency rating for Indianas starting lineup at a whopping 37.4. (By comparison, the Miami Heats starting five this season had a net efficiency rating of 10.9, among the highest in...
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Find hotels in Stourport-on-Severn using the list and search tools below. Explore our guide for inexpensive and discount hotel/motel rates in or near Stourport-on-Severn, United Kingdom for your upcoming personal or group travel. We provide the best Stourport-on-Severn hotel rooms. Review the Stourport-on-Severn hotel list below to find the perfect lodging. We also have a map of the Stourport-on-Severn hotels. Booking 5+ Rooms? Find Stourport-on-Severn Group Hotel DealsThe Stourport-on-Severn center point is approximately 2 miles from West Midland Safari Park and 3 miles from City Centre Kidderminster.
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text:
I am working on my first login script. I have been following examples from a book, but this is where I am stuck. I want the user login to be an e-mail address, but when I would hit submit, I would get this error.
Warning: mysqli_num_rows() expects parameter 1 to be mysqli_result, boolean given in C:\wamp\www\mysite\index.php on line 19
The script works from the book, there's no typos, and from Google, I found that error is given if the original query fails, so I decided to insert a "mysqli_error" to check what is wrong and I got this:
Nah. firstname.lastname@example.com have an error in your SQL syntax; check the manual that corresponds to your MySQL server version for the right syntax to use near '@mysite.com AND password = SHA('password')' at line 1
So, I am almost positive that the @ sign is ending my query early. I have trim and mysql_real_escape_string as a way to clean up the strings, but besides that I have nothing. I have been Googling for a while now and I can't find or figure out how to make an exception for the @ ("at sign") or some kind of work around.
I didn't want this question to be too long or complicated, but I can provide more code if needed.
Edit: Here is the complete code to narrow down the solution.
if (isset($_POST['submit'])) {
$loginEmail = mysqli_real_escape_string($dbc, trim($_POST['loginEmail']));
$loginPassword = mysqli_real_escape_string($dbc, trim($_POST['loginPassword']));
$query = "SELECT user_id, username FROM user_db WHERE email = $loginEmail AND password = SHA('$loginPassword')";
$loginData = mysqli_query($dbc, $query);
if (mysqli_num_rows($loginData) == 1) {
echo 'You win!';
else {
$error = mysqli_error($dbc);
echo 'Nah. ' . $loginEmail . $error;


text:
Applies to
Surface RT, Surface 2, Windows RT 8.1.
Connect Surface RT or Surface 2 to a second screen
You can make photos, videos, and presentations bigger by connecting your Surface to a TV, monitor, or projector. For example, you can connect to an HDTV and watch movies on a big screen or connect to a projector for a presentation.
To connect Surface to another screen, you'll need a compatible cable and adapter. You can even connect to some displays wirelessly. Surface video adapters are available in the Accessories area on this site and in the Microsoft Store.
How can I connect to another display?
To figure out which adapter and cable you need, look at the video ports on your TV, monitor, or projector.
- HDTV. If your TV has an HDMI port, you'll need a HDMI cable and a Surface HD Digital AV Adapter.
- Projector or monitor. Check the projector or monitor for an HDMI port. If you don't see one, use a VGA cable and the Surface VGA Adapter.
- Wireless. You can also connect to wireless displays when Windows detects one nearby. (Not supported for Surface RT.)
A VGA adapter or cable is for video only. Audio will play from your Surface speakers unless you've connected external speakers. For more info, see Surface sound, volume, and audio accessories.
Connect Surface RT or Surface 2 to a wired display
|Step 1:
|Connect a VGA or HDMI cable to the HDMI or VGA port on your TV, monitor, or projector.
|Step 2:
|Connect the other end of the cable to the Surface adapter.
|Step 3:
|Plug the adapter into the HD video out port on the upper-right side of your Surface.
|Step 4:
|Swipe in from the right edge of the screen, and tap Devices.
(If you're using a mouse, point to the lower-right corner of the screen, move the mouse pointer up, and click Devices.)
|Step 5:
|Tap or click Project, and under Project to a connected screen, choose Duplicate, Extend, or Second screen only. For more info about what these options mean, see Set up your screens below.
Connect Surface 2 to a wireless display
With Surface 2, you can connect to some displays wirelessly. (This isn't available for Surface RT.)
|Step 1:
|Swipe in from the right edge of the screen, and tap Devices.
(If you're using a mouse, point to the lower-right corner of the screen, move the mouse pointer up, and click Devices.)
|Step 2:
|Tap or click Project, and tap or click Add a wireless display.
|Step 3:
|Choose the wireless display in the list of devices found, and follow the instructions on the screen.
If you have problems connecting your Surface or have questions about screen resolution, see Trouble connecting to a second screen?
Set up your screens
After you plug in your second monitor or connect a projector, you can choose your screen options.
|Step 1:
| Swipe in from the right edge of the screen, and tap Devices.
(If you're using a mouse, point to the lower-right corner of the screen, move the mouse pointer up, and click Devices.)
|Step 2:
Tap or click Project, and choose what you want to see on each screen:
Move apps between your screens
To do this
Press this
Move Windows Store or desktop apps to another monitor.
Windows logo key +Shift+Right arrow
Windows logo key +Shift+Left arrow
Move desktop apps to the left, center, and right on a monitor.
Windows logo key +Right arrow
Windows logo key +Left arrow
You can also move a desktop app to another screen by clicking the window's title bar and dragging it to the other screen.
Use two or more apps side by side
Depending on the resolution of your screen, you can have two or more apps open side by side onscreen at a time. You can schedule meetings on your calendar while you respond to email, or record a lecture in class with Sound Recorder while you take notes in the desktop.
To use two apps side by side:
|Step 1:
|Open an app that you'd like to use.
|Step 2:
|Slide in from the top of the screen until an opening appears behind the app, then drag the app to the left or right side.
(If you're using a mouse, drag the top of the screen down until an opening appears behind the app, then drag it to the left or right.)
|Step 3:
|Go back to Start and open a second app. It will appear next to the first app.
|Step 4:
|To resize the apps, drag the divider between the apps.
To use three apps side by side:
- If you go back to Start and open a third app, it'll appear on top of the first two. You can tap or click the left or right side of the third app to replace one of the apps underneath it.
- If you want use all three apps on the same screen, drag the third app until an open space appears next to or between the other apps. If a space doesn't open, your screen can only fit two apps at a time.
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text:
I recently went to the first ever PAX Aus, which is also the first PAX outside of the US. Since Ive never been to a PAX before, I just got a one day (Saturday) pass so I could check it out and see what I thought. I can safely say that after my one day there this year, I already want to go at least two days, maybe even all three next year.
I go to many cons in Melbourne each year, Supanova, Oz Comic Con, Armageddon etc, and I can safely say that PAX was the most well organized convention I have ever been to, and probably the most fun Ive ever had in one day at a con. The biggest difference I can point out between those other cons and PAX, was that while PAX had a limited amount of tickets which sold out, the other cons usually allow on the day arrivals to get in, which means the convention centre ends up over packed and crowded to the point of being uncomfortable with ridiculous lines.
PAX on the other hand was quite spread out and felt a lot more relaxed. Sure there were some lines, especially for panels and Occulus Rift, but overall there was space to breathe and move, which is refreshing for a convention. Even the food lines werent very long in the middle of the day.
The Expo Hall was great fun, and I loved checking out the indie developer section as well as the massive LoL stage in which live tournaments were being held all day. While Nintendo had a large section, I wish Sony and Microsoft also had a presence there. Microsoft did have a glass case which supposedly housed the Xbox One at some points, but I never saw it in there and it wasnt even playable. Maybe next year we can hope to see more from these two other giants of gaming.
The console and PC freeplay areas were also really well organized and I had a lot of fun in there playing a few games with my friend, including some games that Ive never played before. I also didnt expect to leave PAX with an interest in TCG or tabletop gaming, but now I have 5 decks of Magic: The Gathering and am very eager to learn about the game, despite never really playing card games before.
My only real complaint for PAX is the fact that they underestimated just how popular some panels would be. At one point I heard an organizer say "we didnt expect it to be this popular, so we put it in a small venue" which seemed to be the case with many panels. I only managed to get into one panel, which was the Good Game panel. I lined for about 3 hours and only just made it in with a terrible seat up the back, while many others couldnt get in at all. Good Game especially is a panel that could have filled the biggest hall, yet they had us all squashed into a tiny room.
Things like that are to be expected for the first ever Australian PAX though. These issues will be ironed out over the years as the organisers get a better idea of how PAX Aus works and what the people want. As for me, Im already excited for next year.


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The Adenomatous polyposis coli (APC) tumour suppressor is found in multiple discrete subcellular locations, which may reflect sites of distinct functions. In Drosophila epithelial cells, the predominant APC relative (E-APC) is concentrated at the apicolateral adherens junctions. Genetic analysis indicates that this junctional association is critical for the function of E-APC in Wnt signalling and in cellular adhesion. Here, we ask whether the junctional association of E-APC is stable, or whether E-APC shuttles between the plasma membrane and the cytoplasm.
We generated a Drosophila strain that expresses E-APC (dAPC2) tagged with green fluorescent protein (GFP-E-APC) and we analysed its junctional association with fluorescence recovery after photobleaching (FRAP) experiments in live embryos. This revealed that the junctional association of GFP-E-APC in epithelial cells is highly dynamic, and is far less stable than that of the structural components of the adherens junctions, E-cadherin, α-catenin and Armadillo. The shuttling of GFP-E-APC to and from the plasma membrane is unaltered in mutants of Drosophila glycogen synthase kinase 3 (GSK3), which mimic constitutive Wingless signalling. However, the stability of E-APC is greatly reduced in these mutants, explaining their apparent delocalisation from the plasma membrane as previously observed. Finally, we show that GFP-E-APC forms dynamic patches at the apical plasma membrane of late embryonic epidermal cells that form denticles, and that it shuttles up and down the axons of the optic lobe.
We conclude that E-APC is a highly mobile protein that shuttles constitutively between distinct subcellular locations.
The Adenomatous polyposis coli (APC) protein is an important tumour suppressor in the colonic epithelium [1]. A key function of this highly conserved protein is to antagonize Wnt signalling, by constitutively downregulating the transcriptional activity of β-catenin/Armadillo, a key effector of the Wnt signalling pathway [2]. Loss of this function is thought to be critical in the initiation of colorectal tumorigenesis as it causes a transcriptional switch in the intestinal epithelium towards actively dividing crypt progenitor cells [3-5]. APC proteins are highly conserved among vertebrates and flies, and flies encode two APC proteins with overlapping roles in Wnt signalling during development [6,7].
However, APC proteins have additional functions in connection with the actin and microtubule cytoskeletons that appear to be separate from their function in controlling Wnt signalling [8,9]. One of these functions is a role of APC in facilitating cellular adhesion, as indicated by studies in Drosophila tissues [10] and in mammalian colorectal cancer cells [11]. This function in cellular adhesion is likely to be conferred by the subcellular pool of APC protein that is associated with adherens junctions (AJs) in Drosophila [12,13] and in polarised mammalian cells [14]. The mechanism by which APC facilitates cellular adhesion is unknown.
In order to explore this mechanism, we asked whether Drosophila E-APC (also called dAPC2) might have a structural role at AJs. If so, E-APC would be expected to be stably associated with AJs, similarly to the structural components of the adhesive complex. As in mammalian epithelia [15,16], the main functional components of this complex in Drosophila are the transmembrane protein E-cadherin, a calcium-dependent trans-membrane adhesion molecule, and the catenins (Armadillo and α-catenin) that link E-cadherin to the actin cytoskeleton at the cytoplasmic side [17-22]. We thus conducted photobleaching experiments on live embryos expressing E-APC or structural AJ components tagged with green fluorescent protein (GFP) [23-25], to compare their relative mobility. These experiments revealed that GFP-E-APC is less stably associated with AJs than their structural components. We also found that GFP-E-APC is remarkably mobile in neurons.
Results and discussion
We used the GAL4 system to express GFP-E-APC throughout the embryo, and found that its subcellular distribution is very similar to that of endogenous E-APC in fixed embryos. In particular, GFP-E-APC is concentrated underneath the plasma membrane in apicolateral regions of embryonic epithelial cells (Fig. 1a; Fig. 2b). These regions form the zonula adherens (ZA) which contains the AJs [26]; they can also be visualised with antibody staining against α-catenin [27] (Fig. 1b), and we observe a remarkably close coincidence of GFP-E-APC and α-catenin. Similar results were obtained by Akong at el. who examined the same GFP-E-APC transgene in the embryo [7], and who also showed that GFP-E-APC is distributed similarly as endogenous E-APC in larval neuroblasts [28].
Figure 1. Association of GFP-E-APC with AJs of embryonic epithelial cells. Side view of epidermis of a ~6 hour old embryo expression ubiquitous GFP-E-APC, stained with antibody against (a) GFP and (b) α-catenin to mark the apicolateral AJs; note the co-incidence of GFP-E-APC and α-catenin staining (arrows).
Figure 2. FRAP protocol for the analysis of live Drosophila embryos expressing GFP-E-APC. (a) Sketch of the microscopy and data analysis used to determine the mobility of GFP-E-APC in epithelial cells of live embryos. (b) Consecutive face-on views of a live ~6 hours old embryo (stage 11) expressing GFP-E-APC, with squares marking specific sections of the AJs at cell interfaces; the set of yellow squares in the right-hand image illustrate the cell shape changes that took place during the 5 minutes between the two optical sections shown. White bar in this and subsequent figures, 5 μm.
Next, we conducted fluorescence recovery after photobleaching (FRAP) experiments in live embryos, to examine how stably GFP-E-APC is associated with adherens junctions. We bleached the fluorescence in a defined square centred over the junctional region of an epithelial cell with a short laser pulse, and examined the recovery of the fluorescence within this square over time (Fig. 2a). This revealed a relatively fast rate of recovery of most of the fluorescence within a few minutes (Fig. 3a) [see 1]. Quantitative analysis shows that nearly 80% of the initial fluorescence is recovered within ~220 seconds (with a half-time value of ~60 seconds) (Fig. 4a,4e). This indicates that the association of E-APC with the ZA is dynamic rather than stable. The fluorescence recovery observed in these FRAP experiments could be due to movement of E-APC between the cytoplasm and the plasma membrane, but also to sideways movement along the plasma membrane (see also below).
Figure 3. FRAP of GFP-E-APC and GFP-tagged AJ proteins in early embryonic epithelial cells. Face-on views of live ~6 hours old embryos (stage 11) expressing (a) GFP-E-APC, (b) E-cadherin-GFP, (c) Armadillo-GFP, (d) α-catenin-GFP, with white squares marking sections of cell interfaces that were bleached, and red squares marking unbleached control sections. Pre-bleaching images are shown on the left; subsequent images on the right show recovery of fluorescence within white squares 15, 75 and 300 seconds after bleaching [see additional file 1].
Additional File 1. FRAP of GFP-E-APC in early embryonic epithelial cells. Example of a FRAP experiment of GFP-E-APC, as described in Figure 3.
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Figure 4. Quantitative evaluations of FRAP experiments. Plots of the relative fluorescence in white squares compared to grey squares in Fig. 3 as a function of time; error bars indicate the range of values from >12 different experiments. (a-d) FRAP of individual GFP-tagged proteins in wild-type embryos, as indicated; (e) combined data of (a-d); (f) Comparison of FRAP of GFP-E-APC in wild-type and sgg mutant embryos (note that we were unable to distinguish between null mutant and paternally rescued embryos; however, the rescue activity of the paternal allele is minimal as both types of embryos are highly abnormal).
We also conducted FRAP experiments with structural AJ components, namely E-cadherin-GFP, Armadillo-GFP and α-catenin-GFP. In these cases, we can only recover a small fraction of the initial fluorescence within the time frame of the experiment (Fig. 4a,4b,4c,4d,4e; note that these experiments cannot be extended beyond ~6 minutes, due to the extensive cell shape changes during this developmental stage). Furthermore, the rate of recovery is slower than that observed with GFP-E-APC, with estimated half-times of >3 minutes (α-catenin-GFP and of E-cadherin-GFP; Fig. 4b,4d,4e). This also appears to be true for Armadillo-GFP (Fig. 4c,4e), though we cannot estimate its half-time of recovery with confidence, given that its fluorescence levels are considerably lower than that of the other GFP-tagged protein examined in this study.
We conclude that E-APC is significantly more mobile than the structural AJ components. This suggests that E-APC shuttles either within the cortex, along the zonula adherens, or that it shuttles from the cytoplasm to the plasma membrane (as previously proposed; [29]). Interestingly, the observed rates of recovery of GFP-E-APC were much slower than the estimated rate of free diffusion (e.g. [30]; the rate of recovery of GFP alone was <10 seconds, i.e. too fast to be measured by our experimental setup). This suggests that the movements of GFP-E-APC are primarily determined by the kinetics of its binding to ligands. One of these could be Axin which associates with E-APC in Drosophila cells to from large dot-like structures [31]. Similarly, Axin associates with APC in mammalian cells to form large molecular weight protein complexes [32]. Our observations argue against a structural role of E-APC in cellular adhesion. However, they are consistent with a catalytic role of E-APC in facilitating cellular adhesion, for example by maintaining the junctional pool of Armadillo [10,29,33]. In support of this, recent evidence suggests that there is rapid exchange of β-catenin within the junctional cadherin complex, and that APC is required for this process [34].
In late embryonic stages, GFP-E-APC forms striking patches underneath the apical plasma membrane of epidermal cells that are in the process of forming denticle extrusions (Fig. 5). These striking 'pre-denticle' patches are also seen in embryos stained with antibody against E-APC, and overlap with actin patches [25]. They may thus represent an actin-dependent association of E-APC as that seen in the cortex of earlier epithelial cells and at the ring canals between nurse cells within the egg chambers [25,33]. FRAP experiments revealed that the presence of GFP-E-APC in these pre-denticle patches is also dynamic, with an estimated half-time of fluorescence recovery of 200–300 seconds (Fig. 5). Again, E-APC is therefore unlikely to have a structural role in these patches.
Figure 5. FRAP of GFP-E-APC patches in late embryonic epithelial cells. Face-on views of ~17 hours old embryo (stage 17) expressing GFP-E-APC, showing patches of GFP-E-APC at the apical plasma membrane of epidermal cells forming denticles (pre-bleach and subsequent images labelled as in Fig. 3). Note the fast recovery of the fluorescence in these patches after photobleaching.
It has been reported that E-APC and Armadillo are required for anchoring mitotic spindles in the cortex of dividing blastoderm cells in the early Drosophila embryo [25]. We cannot measure the kinetics of GFP-E-APC association with the cortex in these early embryonic cells, because of insufficient expression levels at this stage. However, assuming that these kinetics do not change radically during embryonic development, our observations from the later embryos (Fig. 3,4,5) suggest that E-APC has a catalytic role in capturing microtubules in the cellular cortex, rather than providing a structural tether [25].
We also expressed GFP-E-APC in eye imaginal discs, to examine its subcellular distribution within a larval epithelial sheet. We thus noticed striking puncta of green fluorescence within the axons of the optic stalk that connects these discs to the larval brain (Fig. 6). These puncta resemble the E-APC/Axin-GFP dots that we observe in embryonic cells [31] and in these axons (not shown), and also the E-APC/Armadillo dots that Peifer and colleagues observed in early embryos [25]. They may thus represent the Axin destruction complex [31]. We performed FRAP experiments, bleaching a 6 μm wide strip perpendicularly across the axons and monitoring the recovery of the fluorescence into the bleached section. This revealed that the GFP-E-APC puncta are remarkably dynamic: they re-appear within the bleached area within a minute, with an estimated half-time of ~100 seconds (Fig. 6) [see 2]. Many of these puncta seem to re-appear from other focal planes, so we cannot be absolutely certain that they represent movement of existing puncta. However, some of the puncta re-appearing in the bleached area can clearly be traced as moving puncta within the same focal plane (e.g. see isolated axon, left-hand side of [2]). The movement of these GFP-E-APC puncta may be due to tracking (e.g. along microtubules), although we cannot see uni-directionality of movement (i.e. the movement appears to be up and down the axons). The movement we observe in these axons is somewhat reminiscent of that observed with a GFP-tagged truncation of Xenopus APC that misses its C-terminus (thus resembling the overall structure of E-APC): this truncation, despite lacking the putative microtubule-interacting domain within its C-terminus, forms large puncta that can track along microtubules in Xenopus tissue culture cells [35].
Figure 6. FRAP of GFP-E-APC in the larval optical stalk. Optical sections through the optical stalk of a third instar larva, before and after photobleaching, showing fluorescent puncta in individual axons, and the reappareance of these puncta (arrows) from both sides of the bleached areas within minutes [see additional file 2]. Width of bleached strip, 6 μm.
Additional File 2. FRAP of GFP-E-APC in the larval optical stalk. Example of a FRAP experiment of GFP-E-APC, as described in Figure 6.
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GSK3 is inhibited during Wnt signalling [2], and GSK3 mutants in Drosophila (shaggy/zeste white3, or sgg, mutants) therefore mimic constitutive and sustained Wingless signalling [36]. The normal level of Wingless signalling in the embryonic epidermis does not appear to change the subcellular distribution of bulk E-APC protein [12], although it does cause a re-location of Axin-GFP/E-APC complexes to the plasma membrane [31]. However, a reduction of cortical E-APC has been observed in early sgg mutant embryos [12,25]. Likewise, in older sgg mutant embryos, the levels of membrane-associated GFP-E-APC are also noticeably reduced (Fig. 7a,7b,7c). However, this does not appear to be due to a change in mobility of GFP-E-APC since the kinetics of fluorescence recovery between wild-type and sgg null mutant embryos were comparable (Fig. 4f). Instead, it is due to a reduction of the overall E-APC protein levels in these mutants: Western blot analysis of 2–16 hour old embryos revealed that the total levels of GFP-E-APC protein were much lower in sgg mutant embryos compared to the wild type (Fig. 7d). The same is true for endogenous E-APC whose levels are also substantially reduced in sgg mutants (Fig. 7e). This indicates that sgg is required for the stability of E-APC protein, and it suggests that sustained Wingless signalling may destabilise E-APC. Similarly, phosphorylation by GSK3 is required for the stability of mammalian Axin, a functional binding partner of APC [37], and the levels of Drosophila Axin in embryos are also reduced after prolonged Wingless signalling [38]. Destabilisation of the main components of the Axin complex (Axin and APC) during Wnt signalling may be a positive feedback mechanism resulting in the amplification of the signalling level.
Figure 7. Destabilisation of E-APC in sgg mutant embryos. (a, b) Face-on and (c) side views of ~14 hours old embryos (stage 16), fixed and co-stained with antibodies against GFP and α-catenin as indicated, revealing junctional association of GFP-E-APC in (a) wild-type and (b, c) sgg mutant embryos (similar in sgg null and paternally rescued embryos, see also Fig. 4f). (d, e) Western blots of hand-picked 10–16 hours old wild-type and sgg mutant embryos (~100 embryos per lane), probed with antibodies against (d) GFP or (e) E-APC, and α-tubulin as internal controls. Note that the levels of GFP-E-APC and of endogenous E-APC are much reduced in sgg compared to wild-type embryos (sgg mutants represent a 1:1 mixture of sgg null and paternally rescued embryos). The lower bands in upper panels (d, e) correspond to breakdown products of GFP-E-APC and E-APC, respectively; their occurrence varies somewhat between preparations.
The subcellular distribution of E-APC and its accumulation at the adherens junctions is unchanged in other mutants of the Wingless signalling pathway (including wg, axin, dsh and signalling-defective arm mutants; [31]; F. Hamada, X. Yu and M. B., unpublished observations). We thus did not expect any of these mutants to affect the shuttling behaviour of GFP-E-APC to and from the plasma membrane. In support of this, preliminary FRAP experiments indicated that the kinetics of fluorescence recovery are unaffected in dsh null mutant embryos (not shown). Taken together with our results from the sgg mutants, this suggests that the kinetic association of GFP-E-APC with the plasma membrane is unaffected by Wingless signalling.
Our FRAP experiments provided evidence that E-APC is a cytoplasmic shuttling protein whose association with the adherens junctions is highly dynamic. The speed of its shuttling to and from the plasma membrane appears to be constitutive and does not require GSK3 activity. The dynamic association of E-APC with the plasma membrane is consistent with a catalytic role of E-APC, and argues against a structural or tethering role in the cell cortex.
Fly strains
Fly lines transformed with UAS.GFP-E-APC (full length E-APC tagged with GFP at its N-terminal end, inserted into pUAST [39]) were generated by R. Rosin-Arbesfeld (see also [7,28]). The GAL4 driver lines arm.GAL4 and GMR.GAL4 (FlyBase) were used to express GFP-E-APC throughout the embryonic epidermis [31] and in the larval eye disc, respectively. All fly strains were cultured at 25°C.
zw3M11-1 and dshv26 mutant embryos lacking maternal and zygotic gene function were generated as described [40]. We did not detect any differences in the subcellular localisation of GFP-E-APC or α-catenin between zygotic null and paternally rescued sgg mutants (identified with an RFP-marked X chromosome [41]). For Western blot analysis, 10–16 hours old wild-type and sgg mutant embryos were hand-picked (from timed egg collections) under the dissecting microscope, and separated into GFP-positive and GFP-negative embryos; unfertilised embryos were discarded.
Analysis of fixed embryos and Western blots
Antibody staining of fixed embryos and analysis by confocal microscopy were described previously [12]. The following primary and secondary antibodies were used: rabbit anti-E-APC [12], rabbit anti-GFP [14], rat anti-α-catenin [42]; goat anti-rat IgG Alexa Fluor 568, goat anti-rabbit IgG Alexa Fluor 488 (Molecular Probes).
The following primary and secondary antibodies were used for Western blotting: rabbit anti-E-APC [12]; mouse monoclonal anti-GFP IgG2a (Santa Cruz Biotechnology); mouse anti-α-tubulin (clone B-5-1-2, Sigma), as internal control; goat anti-mouse and anti-rabbit HRP IgG (Santa Cruz Biotechnology). The enhanced chemiluminescence (ECL) Western blotting system (Amersham) was used for detection [43].
Live imaging of embryos
For live imaging, embryos were dechorionated in 50% bleach for 1–2 minutes and washed. Embryos were transferred to a moistened black filter (Schleicher and Schüll). Embryos were adhered to coverslips with heptane glue, made by mixing heptane and clear sellotape (Sellotape Ltd). Embryos were mounted in Voltalef oil (10S). For short term imaging (<30 minutes), embryos were mounted on a glass slide with small coverslips as supports. For longer term imaging, e.g. for bleaching of pre-denticle patches, embryos were mounted in oil and placed on Bio-foil gas permeable membrane (Sartorius Ltd) mounted on a perspex frame [44].
Photobleaching of live embryos
FRAP experiments were performed using a Bio-Rad Radiance confocal microscope with a 40× NA 1.3 objective lense. Imaging was performed with a 488 nm argon laser at 5% laser power and the following confocal settings: iris at 4 mm, 50% gain, zoom 10, scan speed 500 lps, box size 512 × 512 pixels. These conditions were found to give minimal photobleaching over the observed time.
For each FRAP experiment, a pre-bleach image was recorded by selecting a focal plane and taking a Z-series, consisting of 3 0.5 μm steps either side of the desired focal plane (from -1.5 μm to +1.5 μm). The LaserSharp software was used to define several regions of interest (ROIs) for bleaching. A maximum of one bleach ROI was placed in any cell and several cells were always left unbleached. Typically, 3–5 ROIs were bleached in one field of view on one embryo. These regions were bleached at 100% laser power (scanning at 500 lps). 10 bleach scans were found to produce the best results for all constructs. After bleaching, a Z-series was recorded every 15 seconds for 5 minutes. At the time of these experiments, the LaserSharp software did not contain a function for performing this type of 4D bleaching experiment. This problem was overcome by manually switching between imaging and bleaching settings and manually saving pre bleach images and starting the time course. As a result of this, there was usually a 30–60 second delay between the pre-bleach image and the post-bleach images.
Data analysis
Data sets were analysed with the Bio-Rad LaserPix software. For each time point, the total pixel intensity distribution was compared to the pre-bleach image to select the corresponding region. The two images were then compared by eye to confirm that they did correspond to the same focal plane. The coordinates for the bleach ROIs were used to accurately locate the bleach spots on the pre bleach image, and the mean fluorescence intensity for each ROI was calculated. Several equivalent sized ROIs were also placed on unbleached cells to measure any change in fluorescence due to photobleaching or movement.
To track movement of the cells, an acetate sheet was placed over the computer monitor and each ROI was marked on it as well as the shapes of the cells surrounding it. By aligning the sheet with the appropriate cell shapes, the ROI could be appropriately positioned for each time point. This process was used to position each ROI on the appropriate image for each time point.
Once all ROIs had been placed on the image, the mean fluorescence intensities were calculated for each ROI, and their positions were saved on a copy of the image (See Fig 2.1). Data was exported to Microsoft Excel for analysis. Relative fluorescence was calculated for each bleach area by dividing fluorescence at time (t) by pre-bleach fluorescence. The change in fluorescence was plotted on a graph with Excel. For each construct tested, the data from multiple bleach experiments from multiple embryos were averaged to give the approximate rate of recovery.
Data sets were discarded for any of the following reasons. First, if movement of the embryo in the Z axis took the sample outside the range of the Z-series in any time point. Second, if movement in the X/Y axis was sufficient to move significant numbers of the bleach boxes outside of the observed region. Third, if an ROI ever left the field of view, all data points for that ROI was discarded. Fourth, all data sets were discarded if the intensities of the control ROIs changed dramatically at any point in the experiment, or showed a large general increase or decrease.
Pre-denticle structures were bleached in a similar manner to junctional E-APC described above.
Live imaging of the larval optic stalk
GFP-E-APC was expressed in eye imaginal discs by the GAL4 system, using the driver line GMR.GAL4 (described in FlyBase). Eye discs and brains were dissected from crawling third instar larvae in PBS. Eye discs were teased away from the brain and inverted to reveal the optic stalk. Whole disc/brains were mounted in a drop of PBS under a cover slip, supported by two smaller cover slips. Each disc was observed for no more than 30 minutes.
Photobleaching of the larval optic stalk
FRAP experiments were performed using a Bio-Rad Radiance confocal microscope and Bio-Rad LaserSharp software, using the 100× NA 1.4 objective lens. A narrow strip was bleached across the whole field of view by adjusting the size of the scanning area. These experiments were performed before a FRAP program was available for LaserSharp so bleaching was performed manually, leading to somewhat variable intervals between each stage of the experiment. The region was bleached with the 488 nm line of an argon laser for approximately 20 scans. Time courses were recorded after each bleaching experiment for 5 minutes.
Authors' contributions
A.C. developed and conducted most of the FRAP experiments; J.M. completed the analysis of GFP-E-APC in live and fixed sgg mutant embryos, including the Western blots; M.B. directed the study, helped with the microscopy and drafted the manuscript. All authors read and approved the final manuscript.
We thank R. Rosin-Arbesfeld, H. Oda and M. Peifer for fly strains expressing GFP-tagged proteins, and F. Hamada for help with the Western blots. J. M. is supported by a grant from the Association for International Cancer Research (no. 03–275) awarded to M.B.
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Choose high qualtiy and fashionable Ralph Lauren clothing in Cheap Ralph Lauren Outlet Store Online and enjoy the best prices. Kids clothing sometimes can be difficult to purchase. This is mainly because there are so many different manufacturers in the market these days that declare to pay attention to the promoting of these styles of clothing. Sometimes it is only much easier to choose producer that we believe in and know. For many of us the Ralph Lauren children collections are one that we go along with quality and one that we are able to believe in. Ralph Lauren kids provides a product of clothing that actually includes every kind of clothing that a youngster could ever wish to put on. The Ralph Lauren producer in common has many years of balance behind which results in them being a reliable name by its customers and suppliers as well. So with regards to Ralph Lauren kids clothing, what's their key. We see that youngsters are drawn to these clothing for a lot of the primary factors we are drawn to just about any kind of clothing even though grownups.
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YIELD: Makes 8 cups
Place all ingredients in large pot.
Bring to boil over medium-high heat. Reduce heat to medium, cover, and simmer until apples are soft, stirring occasionally, about 20 minutes. Stir apples until coarse puree forms; discard cloves and ginger. Serve warm or cold. (Can be made 2 days ahead. Cool, cover, and chill.)


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Oil Filters
Can anyone tell me if the oil filters used on the 1200GS and on the 800GS are the same thing?
I see that the parts numbers differ but are they structurally different?
Just checking 'cos I just got a new 1200 as well as an 800 and I will do my own maintenance.
Not sure about the 800 GS but the one on my 800ST and the GSA are the same. There are others you can use for 800 ST from.
Purolator PL 10241?PL25230
Mobil1 M1-102
Bosch 3330
Supertech ST 3614
All these will fit ST You need the lower profile for the GSA due to the skid plate.
Hope this helps some anyways.
The reason for the different numbers is the washer that comes with them for the drain plug are different.
You can always look it up here: http://www.realoem.com
They are the same filter
They are not the same filter, just sayin.
Hmmm. Well BMW shows different part numbers but they sure do look the same. However, k&n, Mann, hiflo and others list the same number for both. Are you just basing the difference off the BMW number or do you know something I don't?
Same filters fit..no problem..and if'n you use K&N..it would be a 164..
Amsoil would be a Eao51
Like i said the different number is due to the plug washer. Filter is the same.
Not saying you couldnt get the 800 filter to fit but I am holding both in my hand currently at the shop. The 800 is a deeper filter with much more space between the threads and the filter housing than the 1200, and the ridges around the rim for a filter wrench are quite different. Hence why I have two different filter wrenches for the 1200 and 800... different filters.
these are OEM filters I am comparing
Well i'll be. The 2 i had here were the same. Not sure what to say. I do use them(OEM) but also used Hi flow and the ones i listed from other suppliers. Some are taller but on ST it is no issue. Threads and diameter all match. I change oil often so am not as worried about the filter element. I have not found any that will actually give you their spec's as to filtration particle size etc. Is it a GS/GSA thing?
Curious. I also had two OEM filters and they were identical looking. Oh well.
Not only were they never the same to begin with, the old stubby F800/650 has been replaced with a longer filter than the original stubby version.
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text:
You are boring."
The words feel like thousands of ribbons being cut inside of my stomach,
your apathy snipping away like the fiercest of scissors,
a punch to the gut that knocks me back a few feet,
sends me hurtling towards the tiled marble floors.
Every spiked letter clawing at me from the inside out,
it hurts to hold myself under the sheets.
B is for the plentiful moments I can't make your heart
beat, beat, beat anymore, no matter how many times
I perform CPR on your blue, lifeless lips.
O is for ordinary, nothing more, nothing less,
just part of a pattern on a dull wallpaper
that sometimes bubbles up in the sweltering heat.
R is for the "our" that no longer seems to function.
And every time I ask "Why," it latches itself onto "our"
along with an S for "selfish",
and everything becomes "Yours" and I don't know the concept
of "mine" anymore,
because I is for invisible. Because I am only invisible to you,
but I have written letters to you in invisible ink,
traced the very outline of my deepest thoughts
And etched them into syllables, words, sometimes coherency.
Carved them into the familiarity in my arms,
And stored them away within the pockets of my faded jeans.
N is for the times I am
Never enough. Whether it be in your eyes, my eyes,
Or the world's. Because I can stand anger and hatred,
I have braved more storms than you will ever realize.
But I cannot handle disappointment,
It finds its ways to my veins, and slices them open,
Charcoal drops of worthlessness dripping,
Turning into ink splatters on a crisp white button-up.
And G is for giving up.
I am really just boring, after all.