text:
Gabriel Aubry steps out with three year old daughter Nahla!
The custody battle between Halle Berry and Gabriel Aubry may be a bitter one, but Gabriel didn't let it show in L.A., when he stepped out with his daughter Nahla.
Gabriel is currently in the middle of a bitter custody war with Catwoman star Halle.
Last month she was back in court with her powerhouse attorney Laura Wasser in the latest round of their feud.
Her lawyer presented the custody judge with a thick folder detailing all of her former partner's alleged wrongdoings, according to U.S. website RadarOnline.com.
The Monster's Ball actress is claiming Gabriel broke the rules of their custody agreement and even went so far as to allege he has been negligent in his care of the youngster.
Diabetic Halle follows a strict diet and is said to enforce similar rules on the toddler, which the insider claimed the model was not been enforcing.
The source said: 'Gabriel said there was a nuclear level melt-down last week because he gave Nahla pizza on one of her 'non-cheat days.'
'He's basically being totally micro-managed and has no freedom when it comes to his daughter.
'Every single time he doesn't follow every rule, or do things by the book, he's slammed.'
Following the court appearance the judge set a further hearing for a later date.
It was only two months ago Halle claimed they were sorting out a custody arrangement for Nahla, but negotiations hit a stalemate.
The pair split last April 2010 after spending just less than five years together as a couple.
I kinda feel for Gabriel. He has Halle running all over town saying he's a horrible father. If he is – then he deserves it… but if he's not, it's gotta be hard to fight someone like Halle.
Either way – Nahla looks pretty happy in these pictures!
Photos: Fame, Source
Filed under Gabriel Aubry, Halle Berry | 3 Comments


text:
Breast Services/Mammography
Related Links
Holy Family Medical Center
Women's Imaging Center
100 North River Road
Des Plaines, IL 60016
The Women's Imaging Center at Holy Family Medical Center offers the perfect combination of advanced technology, convenience, personal care and attention.
Consider the benefits of selecting Holy Family for your imaging needs:
- New, all digital mammography with CAD (computer-aided detection)
- On-site dedicated board-certified radiologists
- Nurse Navigator
- Easy scheduling with same and next day appointments, early morning, evening and weekend hours
- Prompt results and follow-up information for patients who receive diagnostic mammograms
- Additional services, such as bone density scans, ultrasound and more
- Accreditation by the American College of Radiology
- Transportation to and from appointments with our Care-A-Van service (call 847-297-1800, ext. 2626 for more information)
Women's Imaging Center services include:
- All Digital Mammography with CAD (computer-aided detection) is used on patients experiencing breast problems, like a lump or nipple discharge, to create immediate images and manipulate them for better evaluation.
- Screening and Diagnostic Mammography is a preventative test used to identify abnormalities. The mammography unit in the Women's Imaging Center offer the latest in mammogram technology.
- Breast Ultrasounds deliver superb image quality with uniformity and high resolution to enhance detection and aid in diagnosis.
- Ultrasound Guided Automated Vacuum Assisted Biopsy is a less invasive procedure that can help make a highly accurate diagnosis of a breast abnormality without the need for an open surgery.
- Stereotactic Core Breast Biopsy, when needed, is an accurate, minimally invasive procedure to extract questionable tissue. Using X-rays, a computer guides the biopsy needle to the precise location and collects several samples. More accurate and less costly than traditional techniques, stereotactic core breast biopsy requires only a local anesthetic.
- Breast MRI with CAD uses a magnetic field and radio waves to create a composite, 3-D image of the breast to provide detailed images of hard to detect areas. Breast MRI is more commonly used in addition to a mammogram or other breast-imaging test.
- Fine Needle Aspiration (FNA) is a diagnostic procedure used to investigate superficial lumps or masses, as well as drain cysts located in the breasts.
- Bone Mineral Density Test (BMD) is an accurate, painless, non-invasive test to diagnose osteoporosis and determine risk of fracture. Dexa scanning of the spine and hip are used to screen and monitor for osteoporosis.
The No-Excuse Digital Mammogram
Offering walk-in mammograms Mondays through Saturdays – and you don't even need a physician order. Appointments are also accepted if that is your preference.
Presence Holy Family Medical Center offers the most advanced technology in digital mammography and is the first imaging center in northwest Chicago and the surrounding suburbs to welcome walk-ins for a screening mammogram without a physician order. There's no more waiting weeks or even months like at some diagnostic imaging centers. Remember there needs to be 12 months between each screening mammogram.
We're open 6 days a week
Monday – Friday: 8 am – 4:30 pm.
Saturday: 7 a.m. – 3 p.m.
Evening appointments are available by calling 847-813-3273
For all appointments, call 847-813-3273.
Why choose our Center?
- Featuring a spa atmosphere with tranquil music, heated robes and a patient resource center
- Recognized as a Breast Imaging Center of Excellence by the American College of Radiology
- Results within 24 hours of a screening mammogram
- If further testing is needed, an experienced nurse navigator will guide you through the entire process.
Comprehensive Services
We put our patient's needs first and strive to exceed expectations. In addition to screening
mammograms, we offer:
- diagnostic mammograms
- breast ultrasound
- breast MRI
- ultrasound-guided core needle biopsy and stereotactic core biopsy
- bone mineral density test
For more information, call 847-813-3200.

subset: commoncrawl


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|Boxing Champions | Boxing Schedule | Boxing Video | Boxing History | Pound For Pound | Lounge | The Wire | Audio | Arcade
Big Bad Booty Daddy
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Comments Thread For: Zimnoch vs. McCall Early Results From Poland
In a duel of two previously undefeated welterweights, Michal Zerominski (5-0, 1 KO) looked superior to Lukasz Janik (8-1-1, 3 KOs) in a six-rounder and left no doubt as to who was thewinner, scoring a unanimous decision.
Lightweight Krzysztof Cieslak (19-5, 6 KOs) won a thriller against far less experienced but competent opponent Ariel Krasnopolski (2-3). Cieslak was dropped down hard in the opening seconds of the fight after a huge right hand of Krasnopolski but managed to weather the storm and gradually fought his way back to the fight. Krasnopolski started to fade in the middle rounds. Later in the fight, Cieslak was in full control of the contest and finally got the job done with a hard left hook, which had Krasnopolski down and out cold.
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some close rounds in there & it could have gone either way. if this fight was 10 or 12 rounds i could see mccall pulling it out. mccall was slow but he couldn't miss with his jab especially in the last 2 rounds. mccall is 48 & as the writer said he can hang with the second tier HWs for a few more years. zimnoch beats botha he could get a title shot! wach got a title shot beating tye fields & pianeta got one beating mccall & botha...he's undefeated still
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11 Teacher-Approved Crafts for School
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Blinged Out Calculator Cover
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Make math exciting with this Blinged Out Calculator Cover from I Love to Create! This crystal bling decoration can be applied to anything from your calculator cover to your cell phone. Make your accessories sparkle with this cute "geeky craft"!
- Calculator with cover
- Tulip Glam-it-Up PDA Crystal Set in red and clear crystal
- Scrapbook paper or cardstock
- Heart stencil (we used the Fiskars 3" heart shaped template, cutting out the center of the heart using the 2.5" stencil.)
- Aleene's Tacky Dot Runner
- Fiskars Hearts Template and Cutting Tool
- Use template and tool to create open center heart using cardstock or scrapbook paper.
- Use tape runner to add a little stick to the back of the stencil and adhere to the center of the calculator cover. Be sure cover is clean.
- Starting from outside work your way to the center of the heart adding crystals as in photo. Alternate between red and clear crystals until you reach the center red crystal. Remove stencil and wipe any glue residue away.
- Use the crystals to frame the outer edge of your calculator cover. Work only on the hard areas, leave the soft areas uncovered. There are 16 clear crystals at the top and bottom with 15 red crystals directly beneath them. On the sides there are 5 clear crystals on the top sides and 22 clear crystals on the bottom sides. This is a TI-inspire calculator, so adjustments will need to be made if using a different model. This calculator cover is 3"x8".
- To create the x patterns, form a v shape with five clear crystals at the point of your heart. Add a second 10 crystal v shape using the red crystals. Create a 15 crystal v shape using the clear crystals. Repeat this pattern to form a mirror of the top half as in picture. Add 10 red crystals in the open areas of the center crystal x as in picture. Repeat this process at the top of your cover.
- Check back to be sure all crystals are securely applied. Now get to that math homework already!
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Mental health patterns and consequences : results from survey data in five developing countries
The social and economic consequences of poor mental health in the developing world are presumed to be significant, yet are largely under-researched. The authors argue... Show More +
MIGA annual report 2005
This Annual Report records the achievements of the Multilateral Investment Guarantee Agency during fiscal year 2005. MIGAs investment insurance, dispute mediation services,... Show More +
International companies and post-conflict reconstruction - cross-sectoral comparisons
This paper draws on the experience of international companies as a guide to future policy-making. What factors have guided investment decisions in post-conflict countries?... Show More +
Financing and aid management arrangements in post-conflict situations
This paper is concerned with the specific issues of financing modalities and aid management arrangements in post-conflict situations, and advances a number of recommendations... Show More +
Voices of the poor from many lands
This is the final book in a three-part series entitled, Voices of the Poor. The series is based on an unprecedented effort to gather the views, experiences, and aspirations... Show More +
The World Bank in action : stories of development
This pamphlet presents stories of people from local communities, civil society, the private sector, international institutions, and the World Bank who are working together... Show More +
Urban age (6,1) : Urban age - the shadow economy
The shadow economy (Dana Wickware). Microentrepreneurs: the unacknowledged borrowers (Lucy Conger). A conversation with Elaben Bhatt (Margaret Bergen and Paralekha... Show More +
Post-conflict reconstruction : the role of the World Bank
The nature, scale, and proliferation of conflicts--particularly interstate conflicts--in this post-cold war era are undermining development in a wide range of countries,... Show More +


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1000+ Rentals · Vancouver
1000+ Rentals · Vancouver
Located on the 15th floor in Yaletown - a very beautiful, convenient, modern and safe neighborhood. Close to skytrain station which can take you to almost anywhere. Welcome to Vancouver! Learn More
Yaletown-Roundhouse Station within 3 minutes walking distance This is a small private room in which has a single bed is prefect for a person to stay, and I am living in another room. The ideal that you will share the living area and bathroom with me, so it prefers a lady to live in.
Yaletown, Vancouver
Located in a trendy building in the heart of false creek, walking distance from sea wall, olympic village and skytrain. A bright airy spacious (750 sq ft) loft with open concept, full kitchen, queen bed and lounge which can be used for sleeping Learn More
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This little cozy paradise is near a bike path and is located within walking distance to the express bus #135 which is 15mins to downtown Vancouver and Stanley Park, and heads straight to Burnaby SFU in the other direction! Learn More
Hello, I have provided a very detailed description below and photos above. I hope you find the information useful and it will help you plan your trip. Would you like to visit? Then click on the blue "Contact Me" button to request a reservation or notify me of any special circumstances prior to booking. I would love to learn a bit more about you and your visit! Please share: Estimated Arrival time & what brings you to the city. The room is exactly as you see it in the picture. It is located...
Hastings-Sunrise, Vancouver
Modern, newly renovated and furnished bachelor suite on the ground level of a character house. Great cosy space with a garden view. Has a kitchenette with a full fridge, microwave, hotplate, toaster etc. Great funky area walking distance to trendy cafes, restaurants, bars and many great parks. Learn More
Modern, newly renovated and furnished bachelor suite on the ground level of a character house. Great cosy space with a garden view. Has a kitchenette with a full fridge, microwave, hotplate, toaster etc. Great funky area walking distance to trendy cafes, restaurants, bars and many great parks. The space is beautifully renovated and furnished, it feels spacious for a small space. It gets great natural sunlight from a south facing front yard. The entrance door is small (5ft) which is...
Mount Pleasant, Vancouver
Fully furnished Apartment for Rent Downtown Vancouver 2 block away front Water Front Sky Train Station and at the heart of all business and shopping districts. What you get is a beautifully decorated fully furnished 1 bedroom 1 full bathroom 560 sqft Learn More
Internet and Cable TV Heat, Hot water, Air Conditioning Queen Size Bed Living Room Couch Bar Stools Kitchen Ware, Plates and Utensils In suite Laundry Washer & Dryer Machine Laminate flooring Appliances; Refrigerator, Dishwasher, Microwave, Range Iron and Ironing board Walking distance to Canada place, walking distance to Gas Town, Lots of coffee shop, bars, restaurants. Easy to access public transportation. 2 blocks (5 minute walk) to the Water Front Sky Train Station that takes you...
Downtown Vancouver, Vancouver
Our beautiful, spacious two-bedroom condo has two bathrooms and is just three years old. It's a unique building located in the city's West End, minutes from English Bay, Stanley Park, Denman, Robson Street, and convention centre. Quiet and private. Learn More
A 995 square foot, two-bedroom condo with one large bathroom containing a soaker tub and one en suite with a shower. Gorgeous bamboo floors throughout and decorated tastefully. This is a serene environment and very quiet. The unit is one of two on the second floor, and nobody lives above us. The master bedroom has a queen bed with a pillow-top mattress purchased in December 2013. There is an en suite washroom with heated floors. The second bedroom is set up with a toddler bed, so this is a...
West End, Vancouver
Welcome to this 2 bedroom executive condo with spectacular views of Downtown Coal Harbour. The space features an entertainment furnished living room with 65" HD TV, high speed internet, and modern finishing. Full equipped with everything you need! Learn More
Welcome to this spacious 700 sqft condo with 2 bedrooms, 1 bathroom and large entertainment furnished living room. Being on the 26th floor, this unit has an amazing 180 degree view of Downtown Vancouver and surrounding landscape. Entertainment & Connectivity - 65" HD Television with HD content - Super high speed internet wi-fi Furnishings - High end furnishing with complete beddings - Towels and linens are provided Kitchen - Complete kitchenware is provided You will have access to al...
West End, Vancouver
The Cambie Street location is just down the street from Queen Elizabeth Park, and a 5 minute walk from the Langara-49th Ave train station. Downtown Vancouver is 10 minutes away. It's a perfect spot for appreciating the West Coast in a relaxed mood! Learn More
Our apartment is lightly furnished, and is lived in practically. We have both been self-employed in the arts for many years now, and are sociable, however, enjoy our home as a sanctuary of peace and comfort. The Cambie Street location of Langara Gardens is, in fact, leisurely walkable to downtown Vancouver if guests are fit and willing to spend a little time exploring outside of the core. There is an Alliance Francaise down the street showing free Francophone films, as well as a number of...
Oakridge, Vancouver
Newly Renovated, large 1 bedroom apartment in the heart of downtown. Steps away from Granville, Robson and Davie street - restaurants, clubs, bars, shopping. Wi-Fi, two bicycles, iPad, SmartTV's, laundry, parking, complete office set up and more! Learn More
Rustic style apartment. All brand new appliances with luxury finishings including rain-shower, dishwasher, two wall-mounted LED Smart tv's, pillow-top queen size mattress and more. Professionally cleaned prior to each stay. Bedroom sleeps two and daybed with trundle sleeps two. Access to entire apartment, parking and laundry. Two bikes available at no additional charge. Love to help guests see the best of Vancouver. Response rate is immediate. Can be reached by phone, email, text or what...
Downtown Vancouver, Vancouver
This is a great apartment with everything you need. The location can't be beat and you will feel right at home. Learn More
Enjoy the beautiful city and mountain views from the 16th floor. The huge windows offer fantastic light but also privacy during the day because they are mirrored to the outside. The building: 3 fast elevators gym bbq and patio area secure access 30 min temporary parking in the driveway (if available) Apartment: 9' ceilings central heating and air conditioning luxurious linens and towels 42'' TV Cable tv with over 80 channels DVD Player that also plays CD's Soundbar to plug in your ...
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Our one bedroom is on the top floor of a 4 storey walk up. We have a private south facing patio as well as a shared north facing, mountain/ocean view patio. Our place is clean and the whole building is pet free. We have a BBQ on the back patio. There is Wifi that can be accessed on your own device. Coin laundry facilities are accessible between the hours of 9am-9pm on the ground floor. We will be out of town during your visit. We are accessible by phone or email and have nearby f...
Kitsilano, Vancouver
A cool place to call home! This apartment is located at the most dynamic and trendy neighborhood: Yaletown of downtown Vancouver. Breathtaking view from the apartment, gym, and bike room. High security so you will feel safe and secure. Learn More
Breathtaking view from the apartment in modern new building. This very clean, bright and cozy apartment is fully equipped with a full kitchen( including coffee maker, oven, microwave, blender, dishwasher), in-suit laundry, a queen size bed(including linens and towels), a sofa bed that can accommodate one more person, a small office, wi-fi internet, cable, Netflex on HDTV smart TV. I will provide you with one key and fob that allows access to condo, gym and bike storage. Also free underg...
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Bright, clean, modern and spaciously laid out 714 sq feet apartment in trendy Mount Pleasant. Balcony with stunning views of downtown Vancouver. No car? No problem! Walking distance from stores and transit. Secure underground parking included. Learn More
Mount Pleasant, Vancouver
Enjoy the whole apartment all for yourself! Imagine you are on the 11th floor with a nice view! Full kitchen and tea is provided Highspeed internet Premium TV included! Movies on demand FREE!s Laundry, iron, blowdryer..etc. Negotiable check in time Flexible check out time Less than one minute walk to skytrain and buses One bus to UBC and 15 minute skytrain ride to downtown Lots of free parking spaces along the street local parks, restaurants, groceries and other stores in the area 30 min...
Renfrew-Collingwood, Vancouver
Looking for accomodations close to YVR and Canada Line Bridgeport Station? We offer 4 rooms with double-sized beds , a large bathroom , big soaker tub ,shower. WIFI included. Tim Hortons ,A&W ,Fujiya Sushi , IHOP,Denny's ,Korean BBQ ,Moxies are all within 5-10 minutes walking distance of our home. Learn More
A cozy homelike atmosphere with a friendly host ! Please check 3 more rooms listed if you require accomodation for 6-8 people. Discounted rate offered for minimum 1 week. Large bathroom with double sinks , soaker tub with shower ,towels and WIFI I love to chat with my guests and make them feel comfortable in my home and give them good travel tips . Our neighbourhood is located in a quiet cul de sac with no thru traffic. If you choose to take transit from the airport it will cost you $7...
fantastic location! a character apartment in Vancouver's West End, with inlaid wood floors, and a recently renovated kitchen with gas stove. Two blocks away from all the shopping on Robson street, and minutes away from the beach at English Bay as well as Stanley Park. In the heart of downtown on a tree-lined street, you are set to enjoy the city! Easy to access both the Expo & Millenium Skytrain lines (an easy trip in from the airport), and walking distance to the aquabus to Granville Island...
West End, Vancouver
Excellent private bedroom with ensuite & your own balcony in a 2 bedroom condo. Quiet, clean, modern. Shared roof top terrace with breathtaking views of Vancouver inlet & North Shore mountains. Great location, close to beach & public transport. Learn More
Kitsilano, Vancouver
Just a few dozen steps from famous Commercial Drive, this garden suite is located in a brand new duplex. This 400 sq ft well appointed suite with your own patio opens onto a beautiful garden in the hosts backyard. Explore the Drive in style. Learn More
Our garden suite is located just steps from the heart of Commercial Drive. It's brand new, with all new appliances and furniture and located on the lower level of a brand new duplex completed in January of 2014. We're very proud of what we have created for your comfort. Completely appointed with all you will need from the time you arrive, we are confident you will be able to relax and enjoy all that Commercial Drive and all of Vancouver has to offer. As a guest in our garden suite you w...
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You can now invite anyone to meet at your favorite Starbucks.
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65 year old woman | Pittsburgh, PA, USA | Seeking men 57-70 within 100 miles of Bellevue, PA
I am looking for someone to compliment my life. I love to travel and it would be fun to have a travel companion. I look and act younger than I am. I am not set in my ways and am always open to new ideas. I am always seeking knowledge. My ideal mate would also be willing to travel and be open to trying new things. (With the exception of riding on motorcycles and camping in a tent. Been there, done that and am over it.) I do not take life too serious and I am a firm believer that laughter is the best medicine. If you have no baggage or looking for someone who also has none, then I would have to wonder where you have been all this time. It is my opinion, if you have no baggage you must have grown up in a bubble. To me, the important question is, " What did you choose to do about it?" I emptied mine out and now use it for traveling. I refuse to let my past dictate where my future is going. I am currently attending college full-time and working on a degree. It is long overdue and has been on my bucket list for a long time now. I enjoy this tremendously, but it does take a great deal of my time during the school months. I also have been writing poetry for years and on occasion still do. I am trying to learn photography and I am excited about discovering my talent for art through a few art classes. Well, if you are young in spirit, enjoy some of my interests, and are open to trying new ones email me. Thanks for stopping by and reading my profile. Have a great day !!!
Coffee and conversation , Dining out , Movies/Videos , Museums and art , Music and concerts , Exploring new areas , Performing arts , Travel/Sightseeing
Swimming, Walking / Hiking
Don't display my sign
I have Cats, Dogs
I have two older cats and a 3 year old terrier named Sam.
Middle of the Road
My favorite spot in Pittsburgh is the river walk by the stadiums. I also like to walk around McConnells Mills. I plan on going to central or south america one of these days.
TV is a last resort for me. I am a documentary freak, and I love any show that is about space, the ocean, egypt, biographies, and pretty much anything that is nonfiction.
I enjoy movies, museums, live theatre and live baseball games. Travelling is my favorite thing to do.
Don't have time to read much these days except for my text books.
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What she is looking for
|5'4" (162cms)
|5'10" (177cms) to 6'5" (195cms)
|Body type:
|A few extra pounds
|About average, Athletic and toned, Heavyset, A few extra pounds, Stocky
|Blue, Brown, Grey, Green, Hazel
|Dark brown
|Black, Light brown, Dark brown, Blonde, Salt and pepper, Silver, Dark blonde, Grey, Bald
|No Way
|No Way, Occasionally, Yes, but trying to quit
|Social Drinker
|Social Drinker
|Nonprofit / Volunteer / Activist
|No preference
I have worked in the nonprofit sector for many years. It does not matter what field my match has chosen to persue as long as he has empathy for the disadvantaged (both people and animals).
|I'll tell you later
|No preference
|Widow / Widower
|Never Married, Widow / Widower, Divorced
|Have kids:
|Yes, and they live away from home (2)
|Yes, and they sometimes live at home, No, Yes, and they live away from home
|Wants kids:
|No, but it's OK if my partner has kids
|White / Caucasian
|Asian, Latino / Hispanic, Native American, Pacific Islander, White / Caucasian
I am of Irish, German and Native American descent. I have visited Ireland and someday hope to see Germany.
|Spiritual but not religious
|No preference
|Some college
|No preference
I currently attend college full-time pursuing a degree in social work.


text:
Published: May 23, 2012
Sean O'Toole, left, Joshua Kessner and Jim Villani protest the Mahoning County sheriff 's sale of homes that were to be auctioned off Tuesday. O'Toole and Kessner are members of Occupy Youngstown. The Occupy group says land banks should be used to hold and transfer property.
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DMI Office Furniture Keswick Buffet
DMI Office Furniture Keswick Buffet
Weights & Dimensions
|Product Type: Buffet
|Hardware Finish: Antique Brass
|Distressed: No
|Gloss Finish: Yes
|Base Material: Wood
|Top Material: Wood
|Hardware Material: Metal
|Reclaimed Wood: No
|Solid Wood Construction: No
|Number of Items Included: 1
|Scratch Resistant: Yes
|Stain Resistant: Yes
|Tarnish Resistant: Yes
|Display Case: No
|Drop Leaf/Expandable: No
|Mirror Included: No
|Drawers Included: Yes
|Cabinets Included: No
|Shelves Included: Yes
|Doors Included: Yes
|Lighting: No
|Casters: Yes
|Wine Rack: No
|Stemware Rack: No
|Finished Back: Yes
|Commercial Use: Yes
|Recycled Content: Yes
|Eco-Friendly: Yes
|Country of Manufacture: China
|Assembly Required: No
|Additional Parts Required: No
|FSC Certified: Yes
About the ManufacturerDMi Office Furniture where an office is more than a place to work. It's the womb that nurtures ideas, eases communication, and furthers generations. An office is more than a desk and credenza. It exudes lifestyles, work ethic and for many, it is the harbor in the fast-paced world of profitability and shareholder equity.
DMI Office Furniture Keswick Buffet
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From inside the book
34 pages matching George in this book
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Khalil Greene continues to suffer from anxiety issues
Feb 22, 2010, 11:17 AM EDT
The Rangers signed Khalil Greene to a one-year, $500,000 deal this winter, hoping he could replace Omar Vizquel as their
utility guy. Ken Rosenthal reports some bad news, however:
Infielder Khalil Greene is again suffering from anxiety issues, leaving
the Texas Rangers short an infielder as they begin spring training. Greene, 30, will not report with the rest of the team's position
players Tuesday and could miss significant time, according to
major-league sources.
This is really, really sad.
UPDATE: The Rangers' statement on Greene:
"The Rangers fully support Khalil's decision to address this private
matter. Per club policy, we will not comment on his medical situation.
We have agreed to leave the door open for a continued relationship, if
both Khalil and the team desire that in the future.
"We have not put a timetable on a possible return to the club with
the sole focus right now on doing what we can to assist him. The
Rangers will continue to work with Khalil and his representatives to
monitor his situation and interest in rejoining our organization.
"Over the next few days, we expect that Khalil's status will be
more clearly defined. We will also communicate the impact on the 40-man
roster once we've walked through our administrative options."
Evan Grant tweets that the Rangers could either place him on the restricted list or void his contract. I don't know anything special about the Rangers' roster considerations or Greene's health, but I can't imagine them voiding his deal would help all that much with his anxiety . . .
- Shely - Feb 22, 2010 at 3:11 PM
Only the twits tweet on this one. Let the poor guy along. If he has a medical problem, let him & his docs deal with it. For pete's sake, let him alone. We don't need to know who is treating him and when etc. with all the details. Let it lay, and get on the the games.
- Old Gator - Feb 22, 2010 at 3:20 PM
It would be nice if society in general took that sort of attitude to heart. Then we could get through checkout at the supermarket without the tabloid covers making us want to barf.
- Self Improvement - Feb 23, 2010 at 10:53 PM
Thanks for writing a post about this. You have a bunch of fantastic information here on your site. I am very impressed! I try to keep a couple blogs moderately ongoing myself however it is a struggle sometimes. You have done a very nice job with this one. How do you do it?
- The Ol Goaler - Feb 25, 2010 at 3:01 PM
You look at the tabs in the supermarket? I just turn away, man…
I don't know what demons Greene is fighting, but it was painful to watch him try to play last season. Here's hoping he gets the help he needs, whether he ever plays another inning of baseball or not.
- BrownBrooke26 - May 28, 2010 at 11:37 PM
Some specialists tell that credit loans help a lot of people to live the way they want, just because they can feel free to buy necessary things. Moreover, a lot of banks offer student loan for all people.
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Results 1 to 4 of 4
Thread: HTML help... for portfolio site
July 29th, 2005 #1
HTML help... for portfolio site
Okay.. so I want to know how to make the image open in a sized window w/ the picture you click on..
like let's say you have a thumbnail...
you click it and a new window pops up w/ that picture w/ the correct size of the window?
Also how do u make it so when u click on it, it closes...
show me the way....
- Similar Threads
- Art: Best portfolio site?
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- Portfolio Site
Hide this ad by registering as a memberJuly 29th, 2005 #2
javascript...there must be hundreds of tutorials on this...just google and you'll find it.
...and there are probably just as many different solutions as there are tutorials. I would say create a php page for the popup so you don't have to create hundreds of html pages where the only difference is the file used for the image.
July 29th, 2005 #3
Go get Dreamweaver, and all those little options are just a few clicks away.
July 29th, 2005 #4
I do a certain amount of web design so I know some javascript.
Here is the basic coding you need to do this.
This is just a simple page but the coding will be the same for what you need.
<title>Untitled Document</title> - Standard html for a basic page.
 - The main coding that does the work. You shouldn't need to change this. Also you should only need to put this in once.
 - The specific coding for the link and pop up image. You will need to repeat this block of code for each thumbnail and pop up image.
 - The rest of the standard html
If you get all the links to your image files right this should work. I hope this helps.
Have faith, these things should never be easy.
New Artists Seeking Help Come Here - A mutual learning thread for learning the basics of drawing. Everyone's welcome.
The Sketchlings


text:
Nature | Dogs That Changed the World (Part 2) - Preview | PBS
Duration: 00:31
Program: Nature #2412
Broadcast Date: Oct. 9, 2011
Details the explosion of dog types into the roughly 400 breeds known today and outlines dogs' potential role in medical care for human beings. (Part 2 of 2) 10/9/2011Tags:
Nature | Hard-Wired? | PBS (#2412)
Genetics and brain physiology can affect a dog's personality and behavior. 03:09
NATURE | Animal Odd Couples |... (#3003)
After being abandoned by her mother, a baby fawn, Pippin, was adopted by a Great Dane, Kate, and they have been best... 01:04
Nature | Why We Love Cats and... (#2608)
Airs Wednesday, November 30, 2011 at 8:00 p.m. ET/PT. Check your local listings. 00:31
Nature | Speedy Saluki | PBS (#2411)
For 6,000 years, the Bedouin have bred Saluki for speed. 03:22
Nature | "Underdogs" -... (#2307)
Extreme doggie makeover! 00:30
NATURE | Attenborough's Life...
In "Life on Camera," Sir David Attenborough revisits key places and events in his wildlife filmmaking career,... 00:33
NATURE | Attenborough's Life...
In "Understanding the Natural World," Sir David Attenborough shares his memories of the scientists and the... 00:32
NATURE | Attenborough's Life...
In "Our Fragile Planet," Sir David Attenborough reflects on the dramatic impact that human beings have had on the... 00:32
NATURE | An Original DUCKumentary... (#3004)
A look at all that's entailed in getting those duck feathers just right. "An Original DUCKumentary" premieres... 01:20
NATURE | An Original DUCKumentary... (#3004)
A newly hatched wood duckling jumps from her high-up tree nest to reach her calling mother. "An Original DUCKumentary"... 01:20
Nature | Animal Odd Couples | PBS (#3003)
Full Program: Love apparently knows no boundaries in the animal kingdom. 53:00
NATURE | Animal Odd Couples | The... (#3003)
Primatologist and evolutionary biologist Dr. Lauren Brent discusses her research on the social behavior of nonhuman... 01:41
NATURE | Animal Odd Couples | The... (#3003)
A 45-year-old female Aldabra tortoise was chosen by an unlikely suitor: a male Pacific Brant goose. An... 01:12
NATURE | Magic of the Snowy Owl... (#3002)
In the dim glow of the Arctic glow, the true majesty of the snowy owl can be seen in its frozen, white kingdom.... 01:05
NATURE | Magic of the Snowy Owl |... (#3002)
A snowy owl pair has made a nest out on the tundra. The much larger female is definitely sitting on eggs, and before... 01:28
NATURE | An Original DUCKumentary...
Masters of the water and air, they have conquered the globe. From deft dabblers to great divers, these are one of the... 00:31
NATURE | Animal Odd Couples...
(coming Nov. 7, 2012) A goat and a horse are inseparable for 16 years. A dog befriends a wild deer. It's time to... 00:32
Nature | Siberian Tiger Quest | PBS (#3001)
Full Program: Ecologist Chris Morgan embarks on a challenge to find and film a wild Siberian tiger. 55:39
NATURE "Magic of the Snowy...
NATURE takes an intimate look at the snowy owl, a bird made popular by Harry Potter's faithful companion, Hedwig.... 03:21
- Watch more video from this program
Post Date: September 27, 2011


text:
The invention provides a method for purifying DNA from any source in any form. The method comprises the use of water soluble organic solvents when purifying DNA. By using water soluble organic solvents such as ethanol, propanol, and isopropanol, DNA is purified with greater recovery amounts. In addition, the use of water soluble organic solvents eliminates the use of caustic and poisonous compositions such as chaotropes.
1. A method for purifying DNA from solution in the absence of chaotropes which comprises:
a) adding to the solution (i) a hydrophilic surface selected from the group consisting of celite diatoms, silica polymers, magnesium silicate, silicon nitrogen compounds, aluminum silicates, silica dioxide, glass fiber and nitrocellulose, and (ii) a water soluble organic solvent selected from the group consisting of 80-100% isopropanol, 80-100% propanol, 95-100% ethanol, 100% acetonitrile, and mixtures consisting essentially of 20-80% of each of at least two alcohols selected from the group consisting of isopropanol, propanol and ethanol;
b) allowing the DNA to bind to the hydrophilic surface:
c) separating the hydrophilic surface with the bound DNA from the solution;
(d) washing the separated hydrophilic surface with the bound DNA, and;
(e) eluting the DNA from the hydrophilic surface with an elution buffer.
2. The method of claim 1 in which a combination of the solution, organic solvent and hydrophilic surface is agitated prior to separation of the hydrophilic surface.
3. The method of claim 1 in which steps (a), (b) and (c) are repeated at least once prior performance of steps (d) and (e).
4. The method of claim 1 in which washing step (d) is repeated at least once.
5. The method of claim 1 in which the hydrophilic surface is celite diatoms.
6. The method of claim 1 in which the hydrophilic surface is silica dioxide.
7. The method of claim 1 in which the hydrophilic surface is aluminum silicates.
8. The method of claim 1 in which the water soluble organic solvent is 100% isopropanol.
9. The method of claim 1 in which the water soluble organic solvent is 100% propanol.
10. The method of claim 1 in which the water soluble organic solvent is 100% ethanol.
11. The method of claim 1 in which the water soluble organic solvent is about 80% isopropanol and about 20% water.
12. The method of claim 1 in which the water soluble organic solvent is about 80% propanol and about 20% water.
13. The method of claim 1 in which the water soluble organic solvent is about 60% isopropanol and about 40% ethanol.
14. The method of claim 1 in which the water soluble organic solvent is about 60% propanol and about 40% ethanol.
15. The method of claim 1 which further comprises heating to elute the DNA.
This application is a continuation of application Ser. No. 07/695,113, filed May 3, 1991, now abandoned.
The invention is in the field of molecular biology. In particular, the invention is in the area of deoxyribonucleic acid purification.
The continued advances in molecular biology and related disciplines present continued needs for improvements in tools associated with fully appreciating and developing the advanced technology.
A wide range of technologies involve the use of deoxyribonucleic acids (DNA) in a variety of forms. For example, advances in the area of recombinant DNA technology continually require the use of DNA in the form of probes, genomic DNA, and plasmid DNA.
Advances in the area of diagnostics also continue to utilize DNA in a variety of ways. For example, DNA probes are routinely used in the detection and diagnosis of human pathogens. Likewise, DNA is used in the detection of genetic disorders. DNA is also used in the detection of food contaminants. And, DNA is routinely used in locating, identifying and isolating DNA of interest for a variety of reasons ranging from genetic mapping to cloning and recombinant expression.
In many instances DNA is available in extremely small amounts, and isolation and purification procedures can be laborious and time consuming. The often time consuming and laborious procedures can lead to loss of DNA. In the purification of DNA from specimens obtained from serum, urine, and bacterial cultures, there is the added risk of contamination and false-positive results.
Typical DNA purification protocols involve the use of caustic and poisonous compositions. The typical DNA purification protocol uses high concentrations of chaotropic salts such as sodium iodine and sodium perchlorate.
There are numerous protocols for purifying DNA. As evidenced by recent activity in the area of DNA purification, there is a continued pursuit for optimal DNA purification protocols. U.S. Pat. No. 4,923,978 discloses a process for purifying DNA in which a solution of protein and DNA is passed over a hydroxylated support and the protein is bound and the DNA is eluted. U.S. Pat. No. 4,935,342 discloses purification of DNA by selective binding of DNA to anion exchangers and subsequent elution. U.S. Pat. No. 4,946,952 discloses DNA isolation by precipitation with water-soluble ketones. A DNA purification procedure using chaotropes and dialyzed DNA is disclosed in U.S. Pat. No. 4,900,677.
While the present protocols for purifying DNA are able to accomplish their goal, it is desirable to purify DNA without the use of such caustic and poisonous compounds such as the most often used chaotropes in addition to obtaining increased amounts of DNA.
The present invention provides a method for purifying DNA which comprises the use of non-caustic and nonpoisonous solvents.
In one embodiment is provided a method for purifying DNA from solution which comprises the addition of a water soluble organic solvent to attach DNA to hydrophilic surfaces.
In a preferred embodiment is provided a method for purifying DNA from solution which comprises:
(a) addition of a hydrophilic surface to the solution,
(b) adding a water soluble organic solvent,
(c) separating the DNA solution comprising (a) and (b) into a liquid and non-liquid fraction,
(d) washing the non-liquid fraction of (c),
(e) separating the liquid fraction from the non-liquid fraction in (d), and
(f) removing DNA from the non-liquid fraction of (e).
The invention is especially useful in obtaining greater amounts of purified DNA. In addition, DNA can be purified by binding any hydrophilic surface. Also, the purification can conveniently be performed at room temperature.
The present invention can be practiced by substituting water soluble organic solvents for the binding buffer suggested in any DNA purification protocol. "Purifying", as used in this document refers to obtaining DNA substantially free of cellular debris, and the like.
The start of any DNA purification or isolation procedure requires obtaining the desired DNA from its source. Typical protocols for obtaining DNA from specimens such as serum, urine and bacterial cultures are well known and routinely carried out. Likewise, the ability to obtain DNA from genomic libraries and the like are routine.
The present invention is directed toward the purification of DNA that has been obtained from the particular source. Where the DNA originated is not the key to practicing the invention. The key to the invention is the ability to purify DNA, once obtained from its source. Typical procedures for obtaining DNA end with a suspension of the DNA in solution. References include those for isolation of DNA from biological samples, Harding, J. D., Gebeyehu, G., Bebee, R., Simms, D., Ktevan, L., Nucleic Acids Research,17:6947 (1989), and Marko, M. A., Chipperfield, R., and Birnboim, H. C., Analytical Biochemistry, 121:382 (1982). Procedures for isolation of plasmid DNA can be found in Lutze, L. H., Winegar, R. A., Nucleic Acids Research 20:6150 (1990). Extraction of double-stranded DNA from biological samples can be found in Yamada, O., Matsumoto, T., Nakashima, M., Hagri, S., Kamahora, T., Ueyama, H., Kishi, Y., Uemura H., Kurimura, T., Journal of Virological Methods 27:203 (1990). Most DNA solutions comprise the DNA in a suitable buffer such as TE (Tris-EDTA), TEA buffer (40 mM Tris-Acetate, 1 mM EDTA), or a lysate.
Once the DNA is obtained in a suitable solution, a binding matrix is typically added to the solution. Generally used binding matrixes are silica in the form of glass or diatoms.
After a binding matrix has been added to the solution of DNA, a binding buffer is added. The present invention uses a binding buffer that is a water soluble organic solvent. The term "water soluble organic solvent" refers to a solvent that has organic characteristics that results in DNA leaving solution.
Preferred steps for practicing the invention with hydrophilic surfaces of particles, beads, and the like, comprise a binding step, a washing step, a drying step and an elution step. The binding step generally comprises the addition of a hydrophilic surface to a DNA containing solution, addition of a solution comprising water soluble organic solvent (order of addition of hydrophilic surfaces or water soluble organic solvent is not critical), agitation, centrifugation, and discarding the liquid fraction. The binding step is usually repeated at least once. The wash step generally comprises the addition of a wash buffer to remove solvent (for example 50% ethanol and 50% (40 mM Tris, 4 mM EDTA, 0.8 N NaCl pH 7.4)), agitation, centrifugation, and the discarding of liquid. The drying step generally comprises drying for about 2 to 20 minutes at about 40-70 degrees C. The elution step generally comprises the addition of an elution buffer (to remove DNA from surface: for example (10 mM Tris, 1 mM EDTA, pH 8.0), vortexing for about 30 seconds, heating for about 10 minutes at about 40-70 degrees C, centrifuging for about 2 minutes and collecting the liquid. At this point the liquid contains the DNA. The elution step is usually repeated at least once.
When practicing the invention with hydrophilic surfaces like filters, preferred steps include a binding step, a wash step, and an elution step. The binding step generally comprises the addition of a water soluble organic solvent to a DNA containing solution, adding through a filter the resultant solution (typically to a well of a blotter or any other filtration system (e.g., syringe filtration)), and optionally passing a water soluble organic solvent through the filter. The filter is briefly air dried (about one minute) after filtering. The wash step generally comprises the addition of a buffer (to remove solvent) through the filter. Generally the filter is briefly air dried (about a minute). The elution step generally comprises removal of DNA from the filter. The area of the filter that was in contact with the solutions is cut out and put in a centrifuge tube. An elution buffer (to remove DNA from filter) is then added followed by heating at about 40-60 degrees C for about 10 minutes. The liquid, which now contains the DNA, is then removed.
Suitable water soluble organic solvents include ethanol, propanol, isopropanol, and acetonitrile. Various concentrations of water soluble organic solvents can also be used in practicing the invention. Preferably the solvent is 100% isopropanol, ethanol or propanol. Most preferably the solvent is isopropanol. Suitable concentrations of water soluble organic solvents include 1% to 100% solutions of ethanol, propanol, isopropanol and acetonitrile. Preferably the concentrations are 20% to 80%. Most preferably the concentrations are 40% to 60%. Typically the variable concentration of solvent is reduced with water, however, combinations of the solvents can also be used. Preferred combinations of solvents include isopropanol and ethanol, isopropanol and propanol, and propanol and ethanol.
Binding matrixes suitable for use in practicing the invention include any hydrophilic surface. Examples of hydrophilic surfaces suitable for use in practicing the invention include nitrocellulose, celite diatoms, silica polymers, glass fibers, magnesium silicates, silicone nitrogen compounds (e.g., SIN4), aluminum silicates, and silica dioxide. The variety of forms that the hydrophilic surfaces can take are also suitable for use in the invention. Suitable forms of hydrophilic surfaces include beads, polymers, particles, and filters (i.e., membranes).
Binding buffers such as the well known chaotropes are believed to cause DNA in solution to attach to hydrophilic surfaces due to the hydration of the chaotrope. The hydration of the chaotrope is believed to reduce the interaction of water molecules with the DNA. The DNA, in turn, is believed forced into interaction with water molecules surrounding the hydrophilic surfaces which results in the DNA attaching to the hydrophilic surface through hydrogen bonding.
While not wishing to be bound or limited by theory, it is believed the present invention reduces the aqueous character of the DNA solution by using a water soluble organic solvent as a "binding buffer". By reducing the aqueous character of the DNA solution it is believed the DNA is forced to interact with the hydrophilic surfaces, thereby effecting a solid phase extraction. In addition, as demonstrated in the Examples section of this document, the invention results in purification by way of binding to a hydrophilic surface and not by way of precipitation.
The invention can be used to purify DNA from a variety of sources and from a variety of forms. Sources of DNA for purification include bacteria, bacteriophage, specimens, plants, animals, and the like. DNA can be found in a variety of forms and includes single-stranded, double-stranded, circular, and linear. The invention can be practiced with DNA from any source in any form.
The following examples illustrate the specific embodiments of the invention described in this document. As would be apparent to skilled artisans, various changes and modifications are possible and are contemplated within the scope of the invention described.
This experiment compares binding properties of different binding buffers to 6M NaClO4 (prep-a-gene). All experiments are in a prep-a-gene matrix (Prep-a-gene kit, Bio-Rad, Richmond, CA) performed under the same conditions except for the substitution of binding buffers.
______________________________________Materials: LOT #______________________________________Polyethyleneglycol Fluka (Fluka 24718584 MW(PEG) Chemical Corp, Ronkon, NY)Urea Fisher (Fisher 895704 Scientific, Norcross, GA)KSCN (potassium Sigma (Sigma 488-0409thiocyanate) Chemical Co., St. Louis, Mo.)Ethanol (E + OH) Fisher 902233Butanol (BuOH) Fisher 890783Glycerol Sigma 104F-0026Guanidine BRL 9DB209HydrochlorideSodium Hydroxide Fisher 862699(NaOH)Ammonium Hydroxide Fisher 860118(NH4 OH)Sulfuric Acid (H2 SO4) Fisher 860102Acetonitrile (CH3 CN) Fisher 890789Sodium Acetate Sigma S-2889 lot 19F-0010(NaOAc)Prep-a-gene kit BioRad Control 41180λDNA (503μg/803μL) BRL (Bethesda 56125A Research Labs, Ground Island,NY)______________________________________
All 13 binding buffers were used with the same conditions.
To each of the thirteen samples was added 20 μL of prep-a-gene diatom solution, followed by 750 μL of binding buffer lightly vortex and incubate 5 minutes at 45 degrees C, centrifuge 2 minutes, discard supernate and repeat binding step. Wash with 500 μL of wash buffer, centrifuge, discard buffer and repeat. Add 25 μL elution buffer, vortex, incubate 5 minutes at 50 degrees C., centrifuge, save supernate, repeat. Gel was run on each of the thirteen samples and the one standard.
The following binding buffers are listed in order of use:
1) Standard 6M NaClO4 (sodium perchlorate) from the prep-a-gene kit
4) 6M Glycerol
5) 95% EtOH
6) 100% BuOH
8) 6M Urea
9) 8M Gaunidine HCl
13) 6M NaOAc
14) Standard λ DNA
Results of gel electrophoresis of the 13 eluted DNA samples compared to the original DNA sample (λDNA) shows ethanol is superior to the 6M sodium perchlorate and all other binding buffers tested for retention of DNA on the solid phase (Prep-a-gene matrix). Acetonitrile was also good.
This experiment expands the results obtained in Example 1. In that experiment EtOH and CH3 CN were shown to be good DNA binding buffers. In this experiment it will be determined how low the % of ethanol, CH3 CN and MeOH can be in the binding buffer and still get good separation or recovery of the DNA. All experiments are done using the prep-a-gene matrix.
______________________________________Materials:______________________________________Prep-a-gene kit BioRadEtOH FisherMeOH FisherCH3 CN Fisher1% agarose gel______________________________________
λ DNA BRL 56125A, 9 Mol 104 503 μg in 803 μL
15 fractions/experiments were done differing only in the binding buffer used. The wash buffer, elution buffer and solid phase were all from a prep-a-gene kit. The procedure is performed in substantial accordance with the teaching of Example 1. 1.3 μl λ DNA is used in each fraction.
__________________________________________________________________________Fractions (diluted with H2 O if not 100%):__________________________________________________________________________1) 100% EtOH (aq) 6) 100% MEOH (aq) 11) 100% CH3 CN (aq)2) 80% EtOH (aq) 7) 80% MeOH (aq) 12) 80% CH3 CN (aq)3) 60% EtOH (aq) 8) 60% MeOH (aq) 13) 60% CH3 CN (aq)4) 40% EtOH (aq) 9) 40% MeOH (aq) 14) 40% CH3 CN (aq)5) 20% EtOH (aq) 10) 20% MeOH (aq) 15) 20% CH3 CN (aq)__________________________________________________________________________
The eluted DNA from the fifteen tested fractions was analyzed by gel electrophoresis and compared to a standard DNA sample (1.3 μL of λ DNA in 48 μL TE buffer (10 mM Tris HCl 1 mM EDTA, pH8.0)). Results indicate 100% ethanol is the best binding buffer with 100% acetonitrile the second best. The more organic character imparted to the binding buffer results in better DNA retention.
This experiment compares the binding abilities of propanol (PrOH), isopropanol (iPrOH) and ethanol (EtOH) and dilutions thereof to each other as well as to NaClO4. The purpose being to optimize the organic effect on the binding of DNA to prep-a-gene matrix.
______________________________________Materials:______________________________________Prep-a-gene kit BioRad Control (kit) 41492, Matrix 40523λDNA (503 μg/803μL) BRL 56125A, 9MOL 1041% agarose gelEtOH Fisher 902233PrOH Fisher 744241iPrOH Aldrich 06208TWDMSO Aldrich 9624HC(dimethylsulfoxide)______________________________________
13 fractions were done. See below for binding buffer used in each of the 13 fractions. All were done with prep-a-gene kit materials, except binding buffers, and prep-a-gene procedure in substantial accordance with the teachings of Example 1.
Binding Buffers Used:
1) 100% propanol
2) 80% propanol 20% H2 O
3) 100% isopropanol
4) 80% isopropanol 20% H2 O
7) 20% propanol 80% ethanol
8) 40% propanol 60% ethanol
9) 60% propanol 40% ethanol
10) 20% isopropanol 80% ethanol
11) 40% isopropanol 60% ethanol
12) 60% isopropanol 40% ethanol
13) Prep-a-gene binding buffer 6M NaClO4
14) Standard DNA (λ DNA)
The eluted DNA samples were analyzed by gel electrophoresis and compared to the standard DNA sample. Results indicate 100% Isopropanol is the best binding buffer. 100% propanol also resulted in good DNA retention. Isopropanol and propanol can be diluted to about 80% in water and still retain DNA. The tests indicate that as the % of isopropanol or propanol in the ethanol dilutions is increased, the amount of DNA retained is also increased.
A lot of the highest weight DNA (closest to well where DNA started) is retained with iPrOH (100%), this is higher than with any other binding buffer used. DMSO retained no DNA.
The following summarizes binding buffers' ability to retain DNA with preferences listed from best to worst based on analysis by gel electrophoresis compared to standard:
______________________________________Retains DNA No Retentions______________________________________1) iPrOH 10% PEG2) EtOH 20% PEG3) 6M NaClO4 6M glycerol4) 60% iPrOH 40% EtOH 6M Urea5) 60% PrOH 40% EtOH 30% NH4OH6) PrOH 10% H2 SO47) A) 40% iPrOH 60% EtOH 6M NaOACB) 40% PrOH 60% EtOH8) A) 80% iPrOH 20% H2 O MeOH 100% orB) 80% PrOH 20% H2 O agueous dilutions9) A) 20% PrOH 80% EtOH EtOH less than 100%B) 20% PrOH 80% EtOH10) 8M guanidine HCl CH3 CN less than 100%11) 6M KSCN DMSO less than 100%12) CH3 CN13) NaI14) BuOH15) 6M Guanidine HSCN16) 6M (NH4)2 SO417) 6M NaCl______________________________________
This experiment compares the binding buffers 6M NaO3 Cl and iPrOH in their ability to stick DNA to a variety of glass fiber membranes.
Gelman Sciences, Inc. filter (Gelman Sciences, Ann Arbor, MI) Type AE glass filter (Lot 603202).
MSI glass fiber filter (Micron Separation, Inc., West Borl, MA) (Lot 19571).
Whatman GF/B (Whatman Ltd., England, UK) Control 7823 Whatman GF/D (Control 4706).
Whatman GF/C (Control 1505). λ DNA (BRL) Lot 9 mo 1104 (503 μg/803 μL) Nitrocellulose (Schleicher & Schuell, Keene, NH) 44031621 Prep-a-gene (Bio-Rad) control 4004.
______________________________________iPrOH Fisher 744241______________________________________
Blotter (Bio/Dot apparatus by Bio-Rad)
Six (6) fractions were prepared identical to each other except that the membrane used to trap the DNA was different in each case. About 1.3 μλ DNA is dissolved in about 248 μL TE buffer. This is diluted with about 750 μL iPrOH and added to the blotter by passing through the filter. After all the liquid is pulled through, air dry about 1 minuteN Add about 750 μL iPrOH again air dry about 1 minute. After all iPrOH is pulled through, add about 750 μL of prep-a-gene wash buffer, pull through and air dry about 1 minute.
Cut the filter where the well comes through. Put cut out portion in centrifuge tube. Add 50 μL prep-a-gene elution buffer. Heat at about 60� C. for about 20 minutes. Results from gel electrophoresis show isopropanol superior for Whatman GF/B, Whatman GF/C, MSI glass, Gelman AE and nitrocellulose. Isopropanol and Gelman AE filters retained about 100% of the DNA.
This experiment determines 1) the effect of pH on DNA binding 2) the effectiveness of CELITE (diatomaceous earth or diatoms) as a binding surface and 3) the effect iPrOH has on DNA sticking to silanized surfaces (i.e., hydrophobic).
Silanized surfaces
1N NaOH
1N HCl
1% agouse gel in 1xTE buffer
TE buffer
Loading dye
λ DNA
7 samples with 248 μL TE buffer and 1.3 μL λ DNA are made. To samples 1-3 is added 1 of 3 silanized surfaces (prep-a-gene matrix, gene-clean matrix (Bio101, La Jolla, CA, and circle prep matrix (Bio 101)) followed by 750 μL iPrOH. Heat at 60� C. for 10 minutes.
During this time, to 3 samples add 20 μL prep-a-gene matrix and to the 4th add 20 μL of a solution of 50% celite 545 (Fisher) and 50% TE buffer. To the celite sample and 1 of the other 3 samples add 750 μL prep-a-gene binding buffer, to 1 sample add 750 μL of prep-a-gene binding buffer pH 11.0, adjusted with 1N NaOH. To one sample add 750 μL prep-a-gene binding buffer pH .1, adjusted with 1N HCl. Heat all 4 for 10 minutes at 60� C.
Centrifuge the 7 samples and decant the binding buffer. Add 750 μL of the same binding buffer to each sample that was used the first time on that sample. Heat at 60� C. for 5 minutes. Centrifuge and decant binding buffer. To each sample add 500 μL prep-a-gene elution buffer, stir/shake 5 minutes, centrifuge, decant, dry at 60� C. for 10 minutes. Add 25 μL prep-a-gene wash buffer, heat at 60� C. for 10 minutes, centrifuge, collect buffer, repeat the elution step.
The eluted fractions were analyzed by gel electrophoresis and compared to standard DNA samples. The results demonstrate that no DNA is recovered from the silanized surfaces, thus, in previous experiments the DNA bound to the surfaces and was not precipitated (a precipitate would not bind the surfaces and would wash away in the wash step).
This experiment compares binding buffer ability to bind DNA to nitrocellulose membranes.
Starting Materials:
Wash buffer (50% EtOH 50% (40 mM Tris 4 mM EDTA 6M NaCl pH 7.4))
Binding buffer (50 mM Tris 1 mM EDTA, 6M NaClO4 pH7.5)
Elution buffer (10 Mmol Trios 1 mM EDTA pH8.0)
Nitrocellulose (5.0 μM AE98 Order #19020 Lot 643317 S&S)
Nitrocellulose (.45 μm BA85 lot #9039/7 S&S)
1% agouse gel in 1X TAE(1X=89 mM Tris-Borate,2 mM EDTA, 89 mM
Boric Acid)
Loading dye
TE buffer
8M guanidine HCl
TBS buffer
Prep-a-gene kit
7 identical samples are made (248 μL TE buffer and 1.3 μL λ DNA). The 7 samples are bound to nitrocellulose membranes using a blotter with exact same procedures as described in Example 4, except for a different binding buffer used each time.
The DNA solution is added to 750 μL of the binding buffer then added to a well. Pull the liquid through and air dry 1 minute. Add 750 μL of the respective binding buffer to the well, pull through and air dry 1 minute. Wash with 750 μL of wash buffer. Pull through and air dry 1 minute.
Cut out the circle below each well and put in a centrifuge tube. Add 50 μL elution buffer, heat at 60� C. for 10 minutes
The eluted DNA samples were analyzed by gel electrophoresis and compared with standard DNA samples. Results show that isopropanol, propanol, and ethanol retain DNA while the chaotropes retain significantly less DNA.
The purpose of this experiment is to determine if λ DNA spiked into a Chlamydia lysate will bind to diatoms using isopropanol as the binding buffer.
Isopropanol (Aldrich, Milwaukee, WI 02610 MW)
Prep-a-gene kit (BioRad 41640)
λ DNA -(BRL 503 μg/803 μL)
Chlamydia (-) lysates:
Chlamydia (-) lysates from Wake County Health Dept.
TE buffer (10 mM Tris-HCl, 1 mM EDTA, pH 8)
TAE buffer (1X)
Ethidium Bromide (10 mg/1 ml stock (Sigma Cat #E-875 Lot #97E-3722)
4% NuSieve agarose in 1xTAE Buffer
ΦX 174 RF DNA/Hae III (BRL Cat #5611SA Lot #940103)
λ DNA/Hind III (BRL Cat #5612SA Lot #9M0104)
Type II Loading Dye (25% Ficoll, 0.25% Bromophenol Blue, 0.25% Xylene cyanol)
______________________________________Electrophoresis Unit: BRL Horizon 58 Submarine UnitPower Unit: Pharmacia Type EPS 500/400Photo Equipment: Polaroid Type 50 Land Camera Polaroid Type 57 Film Fotodyne Light Box UVOther: Siliconized Sterilized Microcentrifuge Tubes Gel/Loading Pipet Tips (Stratagene, LaJolla, CA).______________________________________
Sample, Preparation and Procedure:
13 samples are made, each containing ˜250 μL of one of the Chlamydia (-) human samples listed. To each of these samples is added 10 μL of 1:10 dilution of the λ DNA sample. A 14th sample is made containing 250 μL H2 O and 10 μL of the 1:10 dilution of λ DNA, no Chlamydia (-) human sample is added.
To 5 of the samples and the standard are added 20 μL of prep-a-gene loading matrix followed by 750 μL isopropanol, shake at room temperature 10 minutes. To the remaining 8 samples the isopropanol is added first followed by binding matrix and shaking. The remainder of the experiment was done exactly the same for all 13 samples and the standard.
After shaking samples at room temperature for 10 minutes, centrifuge 1 minute, decant and discard supernate. Wash with 750 μL isopropanol, shake at room temperature 10 minutes, centrifuge, decant and discard supernate. Heat at 50 degrees Centigrade 10 minutes to dry binding matrix. Add 25 μL prep-a-gene elution buffer. Heat at 50 degrees Centigrade 10 minutes, centrifuge 1.5 minutes. Collect supernate, repeat elution step combining eluted factions of each of the 14 samples giving 14 (50 μL) eluted DNA samples. These eluted samples are analyzed by gel electrophoresis to determine if any DNA was eluted.
The experiment demonstrates that DNA can be removed from a sample containing cellular debris (i.e., carbohydrates, proteins, nucleic acids, etc). Both the control λ DNA and human DNA are removed from the sample. The experiment also demonstrates that a number of different protocols can be used with isopropanol as the binding buffer and still get a large percentage of DNA removed from a sample (e.g., heat can be applied in the binding step, or not, two binding steps can be used or one, a wash step can be used with 50% ethanol and 50% low concentration EDTA pH8.0 buffer or no wash. The order of addition of reagents is unimportant, in other words, binding buffer or binding matrix may be added first with no significant changes in the amounts of DNA recovered from the respective sample).
Although the invention has been described with respect to specific modifications, the details thereof are not to be construed as limitations, for it will be apparent that various equivalents, changes and modifications may be resorted to without departing from the spirit and scope thereof, and it is understood that such equivalent embodiments are to be included therein.
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text:
Dinner & Quarter Auction
Event Details
Date(s) & Time(s)::
November 1, 2013 at 6:00 PM
Location: Jerome Senior Center 520 North Lincoln, Jerome, Idaho 83338
Phone: 208-324-5642
Email: firstname.lastname@example.com
Event Description
Join 4-H and the Jerome Senior Center Friday November 1st for our first annual Dinner and Quarter Auction. This fun Family event starts at 6pm with a Potato Bar, Chili and Hot buttered scones. There will be an ongoing silent auction and the live auction begins at 7pm. The cost is $5.00 for an individual or $20.00 per family. All proceeds will go to support the local 4-H clubs and the Jerome Senior Center.Return to event list.
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text: 0 Celebrity Back to article: Kanye West 'looking forward' to fatherhood 0 Previous Next The Saturdays perform at G-A-Y. The show was the last one Rochelle Humes will be taking part in for a while as she prepares to give birth to her first child in the coming weeks. Copyright: WENN Chris Jepson 27 of 44 Previous Next


text:
Buy Tickets Love & Diane: 10th Anniversary Screening
Show From Series
Tuesday, October 21
Wednesday, October 22
Thursday, October 23
Friday, October 24
Saturday, October 25
Sunday, October 26
Monday, October 27
Love & Diane: 10th Anniversary Screening
October 22, 2012
A special 10th anniversary screening of director Jennifer Dworkin's rich, subtle, and moving portrait of a New York City family facing enormous challenges—one of the revelations of the 2002 New York Film Festival. In person: Jennifer Dworkin. Read more »


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Adds pre-compiling support for Ember Handlebars templates to Brunch
This Brunch plugin adds support for pre-compiling Ember Handlebars templates prior to runtime, utilizing the latest and greatest EmberJS build (1.0.0-RC.5).
It is included by default in the Ember Brunch skeleton. However if you would like to import it into your own custom Brunch project, the instructions below will get you up and running.
"ember-handlebars-brunch": "git+ssh://firstname.lastname@example.com:bartsqueezy/ember-handlebars-brunch.git" to
package.json of your Brunch application.
config.coffee, set
precompile: true within the templates compiler if you want to enable pre-compiling.
templates: precompile: true # default is false root: 'templates/' # default is null defaultExtension: 'hbs' joinTo: 'javascripts/app.js' : /^app/
A few reminders about the configuration object mentioned above:
defaultExtension property
root should represent a directory located under your
app directory. If you do not provide a value for this property, ember-handlebars-brunch will, by default, set the template name to the path of your file, starting from
app. For instance, without defining the
root property, a template located at
app/templates/index.hbs will be registered with Ember like
If using the exact example configuration above, your
views and
templates directories should look similar to this:
└─┬ app ├─┬ templates │ ├─┬ index │ │ └── login.hbs │ ├── application.hbs │ └── index.hbs └─┬ views ├─┬ index │ └── login.js ├── application.js └── index.js
Based on the example above, you can define your views like so:
// app/views/application.js AppApplicationView = EmberViewextend templateName: 'application'; // app/views/index.js AppIndexView = EmberViewextend templateName: 'index'; // app/views/index/login.js AppIndexLoginView = EmberViewextend templateName: 'index/login';
The precompiled templates are injected into the
Ember.TEMPLATES namespace. You can access them like so:
var anotherTemplate = EmberTEMPLATES'index/login';
If you wish to
require the template manually instead of using them directly within a view class, you have to use the full path to the file, starting from the templates directory;


text:
As Hurricane Irene slammed into North Carolina this morning, commuters and travelers across the country are feeling its impact.
More than 8,300 commercial airline flights have already been canceled this weekend, and that number is expected to rise, according to the website, Flightware.com.
All airports in the New York area -- Newark, John F. Kennedy and LaGuardia -- will stop accepting domestic and international arrival flights at noon today.
Public transportation in New York City will stop running at noon, and airport officials do not want arriving passengers to be stranded at the airports.
Operations will be suspended at all New York airports at 10 p.m. tonight.
Sam Schwartz, former commissioner for New York City's Department of Transportation said it will take eight hours to get all the trains and buses tucked safely away.
"They're gonna have to move all of the trains - and remember - we never have a time in which all the trains are placed in rail yards," Schwartz said.
Philadelphia International Airport will shut down tonight at 6 p.m.
Most airlines are now giving out travel waivers, which means they do not charge any fees for changing your flight.
But in many cases, you have to book your next flight within a short window.
In the central terminal at LaGuardia Airport, several passengers are stranded in the food court, trying to plot their next move. Many airport shops and restaurants may close at noon.
The Port Authority of New York and New Jersey said it is ready for potential disruptions.
"Additional staff and contractor employees will be assisting customers as needed in every area of airport operations," said the Port Authority, and they will "stock hundreds of cases of bottled water, diapers, cots, blankets and pillows to provide to stranded passengers if necessary.
Under terms of the agency's concession contracts, at least one food vendor in every passenger terminal must remain open 24 hours so that food is available at any time of day or night."
Despite the fact that thousands of flights have been canceled, the departures area at LaGuardia was packed at 6 a.m. this morning with people trying to get home before they get stranded.
Flights will continue to take off as weather permits.
Raleigh-Durham Airport Authority told ABC News Friday morning about 100 out of 400 flights have been canceled for today at RDU and it anticipates further disruptions for flights along the East Coast through Monday, Aug. 29.
How quickly airports can function after Irene will depend on more than clear skies. If the public transportation in New York is not running, many airport personnel may not be able to get to work.
President Obama cut his vacation short by one day, conferencing with his emergency response team, and warned citizens of the dangers of Hurricane Irene.
"I cannot stress this highly enough: If you are in the projected path of this hurricane, you have to take precautions now. Don't wait. Don't delay," Obama warned Friday morning from Martha's Vineyard, urging citizens to visit ready.gov if they are not sure how to prepare.
Amtrak has canceled most trains traveling south of Washington, D.C. for Saturday and Sunday.


text:
Most Popular "Family Relationships" Titles
- Movies or TV
- Genres
- Keywords
- IMDb Rating
- Instant Watch Options
- In Theaters
- On TV
- Release Year
1. Game of Thrones (2011– )
TV-MA | 55 min | Adventure, Drama, Fantasy
Several noble families fight for control of the mythical land of Westeros.
Creators: David Benioff, D.B. Weiss | Stars: Peter Dinklage, Lena Headey, Emilia Clarke, Kit Harington
Votes: 800,035
2. Jupiter Ascending (2015)
PG-13 | 127 min | Action, Adventure, Sci-Fi
A young woman discovers her destiny as an heiress of intergalactic nobility and must fight to protect the inhabitants of Earth from an ancient and destructive industry.
Writers: Andy Wachowski, Lana Wachowski | Directors: Andy Wachowski, Lana Wachowski | Stars: Channing Tatum, Mila Kunis, Eddie Redmayne, Sean Bean
Votes: 91,287
3. Supernatural (2005– )
TV-14 | 44 min | Drama, Fantasy, Horror
Two brothers follow their father's footsteps as "hunters" fighting evil supernatural beings of many kinds including monsters, demons, and gods that roam the earth.
Creator: Eric Kripke | Stars: Jared Padalecki, Jensen Ackles, Jim Beaver, Misha Collins
Votes: 241,188
4. Sons of Anarchy (2008–2014)
TV-MA | 45 min | Crime, Drama, Thriller
A man in his early 30s struggles to find a balance in his life between being a new dad and his involvement in a motorcycle club.
Creator: Kurt Sutter | Stars: Charlie Hunnam, Mark Boone Junior, Katey Sagal, Kim Coates
Votes: 148,472
5. Interstellar (2014)
PG-13 | 169 min | Adventure, Drama, Sci-Fi
A team of explorers travel through a wormhole in space in an attempt to ensure humanity's survival.
Writers: Jonathan Nolan, Christopher Nolan | Director: Christopher Nolan | Stars: Matthew McConaughey, Anne Hathaway, Jessica Chastain, Mackenzie Foy
Votes: 660,473
6. Once Upon a Time (2011– )
TV-PG | 44 min | Adventure, Fantasy, Romance
A woman with a troubled past is drawn to a town in Maine where fairy tales are to be believed.
Creators: Adam Horowitz, Edward Kitsis | Stars: Ginnifer Goodwin, Jennifer Morrison, Lana Parrilla, Josh Dallas
Votes: 144,368
7. Poltergeist (2015)
PG-13 | 93 min | Horror, Thriller
A family whose suburban home is haunted by evil forces must come together to rescue their youngest daughter after the apparitions take her captive.
Writers: David Lindsay-Abaire, Steven Spielberg, Michael Grais, Mark Victor | Director: Gil Kenan | Stars: Sam Rockwell, Rosemarie DeWitt, Kennedi Clements, Saxon Sharbino
Votes: 15,635
8. Back to the Future (1985)
PG | 116 min | Adventure, Comedy, Romance
A young man is accidentally sent 30 years into the past in a time-traveling DeLorean invented by his friend, Dr. Emmett Brown, and must make sure his high-school-age parents unite in order to save his own existence.
Writers: Robert Zemeckis, Bob Gale | Director: Robert Zemeckis | Stars: Michael J. Fox, Christopher Lloyd, Lea Thompson, Crispin Glover
Votes: 614,900
9. Maggie (I) (2015)
PG-13 | 95 min | Drama, Horror, Thriller
A teenage girl in the Midwest becomes infected by an outbreak of a disease that slowly turns the infected into cannibalistic zombies. During her transformation, her loving father stays by her side.
Writer: John Scott 3 | Director: Henry Hobson | Stars: Arnold Schwarzenegger, Abigail Breslin, Joely Richardson, Laura Cayouette
Votes: 12,687
10. Run All Night (2015)
R | 114 min | Action, Crime, Drama
Mobster and hit man Jimmy Conlon has one night to figure out where his loyalties lie: with his estranged son, Mike, whose life is in danger, or his longtime best friend, mob boss Shawn Maguire, who wants Mike to pay for the death of his own son.
Writer: Brad Ingelsby | Director: Jaume Collet-Serra | Stars: Liam Neeson, Ed Harris, Joel Kinnaman, Vincent D'Onofrio
Votes: 37,619
11. Mad Men (2007–2015)
TV-14 | 45 min | Drama
A drama about one of New York's most prestigious ad agencies at the beginning of the 1960s, focusing on one of the firm's most mysterious but extremely talented ad executives, Donald Draper.
Creator: Matthew Weiner | Stars: Jon Hamm, Elisabeth Moss, Vincent Kartheiser, January Jones
Votes: 123,059
12. Lost (2004–2010)
TV-14 | 44 min | Adventure, Drama, Fantasy
The survivors of a plane crash are forced to work together in order to survive on a seemingly deserted tropical island.
Creators: J.J. Abrams, Jeffrey Lieber, Damon Lindelof | Stars: Jorge Garcia, Naveen Andrews, Matthew Fox, Josh Holloway
Votes: 326,979
13. Modern Family (2009– )
TV-PG | 22 min | Comedy
Three different, but related families face trials and tribulations in their own uniquely comedic ways.
Creators: Steven Levitan, Christopher Lloyd | Stars: Ed O'Neill, Sofía Vergara, Julie Bowen, Ty Burrell
Votes: 202,015
14. A Deadly Adoption (2015 TV Movie)
TV-14 | 84 min | Drama, Thriller
A successful couple house and care for a pregnant woman with the hopes of adopting her unborn child.
Writer: Andrew Steele | Director: Rachel Goldenberg | Stars: Will Ferrell, Kristen Wiig, Jessica Lowndes, Debra Christofferson
Votes: 1,427
15. Ray Donovan (2013– )
TV-MA | 60 min | Crime, Drama
Ray Donovan, a professional "fixer" for the rich and famous in LA, can make anyone's problems disappear except those created by his own family.
Creator: Ann Biderman | Stars: Liev Schreiber, Paula Malcomson, Jon Voight, Devon Bagby
Votes: 30,593
16. Homeland (2011– )
TV-MA | 55 min | Drama, Mystery, Thriller
When Marine Nicolas Brody is hailed as a hero after he returns home from eight years of captivity in Iraq, intelligence officer Carrie Mathison is the only one who suspects that he may have been "turned".
Creators: Alex Gansa, Howard Gordon | Stars: Claire Danes, Mandy Patinkin, Damian Lewis, Morena Baccarin
Votes: 188,694
17. Slow West (2015)
R | 84 min | Action, Thriller, Western
A young Scottish man travels across America in pursuit of the woman he loves, attracting the attention of an outlaw who is willing to serve as a guide.
Writer: John Maclean | Director: John Maclean | Stars: Kodi Smit-McPhee, Michael Fassbender, Ben Mendelsohn, Aorere Paki
Votes: 5,895
18. Poldark (2015– )
60 min | Drama, History, Romance
Ross Poldark returns home after American Revolutionary War and rebuilds his life with a new business venture, making new enemies and finding a new love where he least expects it.
Stars: Aidan Turner, Eleanor Tomlinson, Kyle Soller, Heida Reed
Votes: 3,214
19. The Fosters (2013– )
60 min | Drama
Teenager Callie Jacobs is placed in a foster home with a lesbian couple and their blend of biological, adoptive and foster children.
Creators: Peter Paige, Brad Bredeweg | Stars: Teri Polo, Sherri Saum, David Lambert, Hayden Byerly
Votes: 12,139
20. The Godfather (1972)
R | 175 min | Crime, Drama
The aging patriarch of an organized crime dynasty transfers control of his clandestine empire to his reluctant son.
Writers: Mario Puzo, Francis Ford Coppola | Director: Francis Ford Coppola | Stars: Marlon Brando, Al Pacino, James Caan, Diane Keaton
Votes: 1,013,634
21. That '70s Show (1998–2006)
TV-PG | 22 min | Comedy
A comedy revolving around a group of teenage friends, their mishaps, and their coming of age, set in 1970s Wisconsin.
Creators: Mark Brazill, Bonnie Turner, Terry Turner | Stars: Topher Grace, Laura Prepon, Mila Kunis, Danny Masterson
Votes: 102,959
22. Scream (1996)
R | 111 min | Horror, Mystery
A group of teens are pitted against a masked murderer that tests their knowledge of horror movies.
Writer: Kevin Williamson | Director: Wes Craven | Stars: Neve Campbell, Courteney Cox, David Arquette, Skeet Ulrich
Votes: 199,815
23. Shameless (2011– )
TV-MA | 46 min | Comedy, Drama
An alcoholic man lives in a perpetual stupor while his six children with whom he lives cope as best they can.
Creators: Paul Abbott, John Wells | Stars: Emmy Rossum, William H. Macy, Ethan Cutkosky, Shanola Hampton
Votes: 74,977
24. Harry Potter and the Sorcerer's Stone (2001)
PG | 152 min | Adventure, Family, Fantasy
Rescued from the outrageous neglect of his aunt and uncle, a young boy with a great destiny proves his worth while attending Hogwarts School of Witchcraft and Wizardry.
Writers: J.K. Rowling, Steve Kloves | Director: Chris Columbus | Stars: Daniel Radcliffe, Rupert Grint, Richard Harris, Maggie Smith
Votes: 389,849
25. Glee (2009–2015)
TV-PG | 44 min | Comedy, Drama, Music
A group of ambitious misfits try to escape the harsh realities of high school by joining a glee club, where they find strength, acceptance and, ultimately, their voice, while working to pursue dreams of their own.
Creators: Ian Brennan, Brad Falchuk, Ryan Murphy | Stars: Lea Michele, Jane Lynch, Matthew Morrison, Cory Monteith
Votes: 110,997
26. Independence Day (1996)
PG-13 | 145 min | Action, Adventure, Sci-Fi
The aliens are coming and their goal is to invade and destroy Earth. Fighting superior technology, mankind's best weapon is the will to survive.
Writers: Dean Devlin, Roland Emmerich | Director: Roland Emmerich | Stars: Will Smith, Bill Pullman, Jeff Goldblum, Mary McDonnell
Votes: 383,763
27. It's Always Sunny in Philadelphia (2005– )
TV-MA | 22 min | Comedy
Four young friends with big egos and slightly arrogant attitudes are the proprietors of an Irish bar in Philadelphia.
Creators: Rob McElhenney, Glenn Howerton | Stars: Charlie Day, Glenn Howerton, Rob McElhenney, Kaitlin Olson
Votes: 113,549
28. The Judge (2014)
R | 141 min | Crime, Drama
Big city lawyer Hank Palmer returns to his childhood home where his father, the town's judge, is suspected of murder. Hank sets out to discover the truth and, along the way, reconnects with his estranged family.
Writers: Nick Schenk, Bill Dubuque, David Dobkin | Director: David Dobkin | Stars: Robert Downey Jr., Robert Duvall, Vera Farmiga, Billy Bob Thornton
Votes: 104,251
29. Black Work (2015 TV Mini-Series)
60 min | Crime, Drama, Thriller
A policewoman sets out to discover who murdered her husband, who was an undercover police officer.
Stars: Sheridan Smith, Honor Kneafsey, Matthew McNulty, Oliver Woollford
Votes: 100
30. Boyhood (I) (2014)
R | 165 min | Drama
The life of Mason, from early childhood to his arrival at college.
Writer: Richard Linklater | Director: Richard Linklater | Stars: Ellar Coltrane, Patricia Arquette, Ethan Hawke, Elijah Smith
Votes: 211,989
31. The Sopranos (1999–2007)
TV-MA | 55 min | Crime, Drama
New Jersey mob boss, Tony Soprano, deals with personal and professional issues in his home and business life.
Creator: David Chase | Stars: James Gandolfini, Lorraine Bracco, Edie Falco, Michael Imperioli
Votes: 156,409
32. Poltergeist (1982)
PG | 114 min | Horror
A family's home is haunted by a host of ghosts.
Writers: Steven Spielberg, Michael Grais, Mark Victor | Director: Tobe Hooper | Stars: JoBeth Williams, Heather O'Rourke, Craig T. Nelson, Beatrice Straight
Votes: 92,284
33. The Good Wife (2009– )
TV-14 | 60 min | Crime, Drama, Mystery
Alicia has been a good wife to her husband, a former state's attorney. After a very humiliating sex and corruption scandal, he is behind bars. She must now provide for her family and returns to work as a litigator in a law firm.
Creators: Michelle King, Robert King | Stars: Julianna Margulies, Chris Noth, Josh Charles, Matt Czuchry
Votes: 40,738
34. Star Wars: Episode IV - A New Hope (1977)
PG | 121 min | Action, Adventure, Fantasy
Luke Skywalker joins forces with a Jedi Knight, a cocky pilot, a wookiee and two droids to save the universe from the Empire's world-destroying battle-station, while also attempting to rescue Princess Leia from the evil Darth Vader.
Writer: George Lucas | Director: George Lucas | Stars: Mark Hamill, Harrison Ford, Carrie Fisher, Alec Guinness
Votes: 759,993
35. The Simpsons (1989– )
TV-PG | 22 min | Animation, Comedy
The satiric adventures of a working-class family in the misfit city of Springfield.
Creators: James L. Brooks, Matt Groening, Sam Simon | Stars: Dan Castellaneta, Nancy Cartwright, Julie Kavner, Yeardley Smith
Votes: 218,391
36. Halt and Catch Fire (2014– )
60 min | Drama
Set in the early 1980s, this series dramatizes the personal computing boom through the eyes of a visionary, an engineer and a prodigy whose innovations directly confront the corporate ... See full summary »
Creators: Christopher Cantwell, Christopher C. Rogers | Stars: Lee Pace, Scoot McNairy, Mackenzie Davis, Kerry Bishé
Votes: 10,826
37. Back to the Future Part II (1989)
PG | 108 min | Adventure, Comedy, Sci-Fi
After visiting 2015, Marty McFly must repeat his visit to 1955 to prevent disastrous changes to 1985... without interfering with his first trip.
Writers: Robert Zemeckis, Bob Gale | Director: Robert Zemeckis | Stars: Michael J. Fox, Christopher Lloyd, Lea Thompson, Thomas F. Wilson
Votes: 281,526
38. Californication (2007–2014)
TV-MA | 28 min | Comedy, Drama
A self-loathing, alcoholic writer attempts to repair his damaged relationships with his daughter and her mother while combating sex addiction, a budding drug problem, and the seeming inability to avoid making bad decisions.
Creator: Tom Kapinos | Stars: David Duchovny, Natascha McElhone, Evan Handler, Pamela Adlon
Votes: 131,518
39. The Princess Bride (1987)
PG | 98 min | Adventure, Comedy, Family
While home sick in bed, a young boy's grandfather reads him a story called The Princess Bride.
Writer: William Goldman | Director: Rob Reiner | Stars: Cary Elwes, Mandy Patinkin, Robin Wright, Chris Sarandon
Votes: 261,487
40. This Is Where I Leave You (2014)
R | 103 min | Comedy, Drama
When their father passes away, four grown siblings are forced to return to their childhood home and live under the same roof together for a week, along with their over-sharing mother and an assortment of spouses, exes and might-have-beens.
Writer: Jonathan Tropper | Director: Shawn Levy | Stars: Jason Bateman, Tina Fey, Jane Fonda, Adam Driver
Votes: 39,872
41. Arrested Development (2003– )
TV-PG | 22 min | Comedy
Level-headed son Michael Bluth takes over family affairs after his father is imprisoned. But the rest of his spoiled, dysfunctional family are making his job unbearable.
Creator: Mitchell Hurwitz | Stars: Jason Bateman, Michael Cera, Portia de Rossi, Will Arnett
Votes: 184,442
42. Dawn of the Planet of the Apes (2014)
PG-13 | 130 min | Action, Drama, Sci-Fi
A growing nation of genetically evolved apes led by Caesar is threatened by a band of human survivors of the devastating virus unleashed a decade earlier.
Writers: Mark Bomback, Rick Jaffa, Amanda Silver, Pierre Boulle | Director: Matt Reeves | Stars: Gary Oldman, Keri Russell, Andy Serkis, Kodi Smit-McPhee
Votes: 264,173
43. 90210 (2008–2013)
60 min | Comedy, Drama, Romance
A Kansas family relocates to Beverly Hills, where their two children adapt to the infamous social drama of West Beverly Hills High.
Creators: Darren Star, Jeff Judah, Gabe Sachs, Rob Thomas | Stars: Shenae Grimes-Beech, Tristan Wilds, AnnaLynne McCord, Jessica Stroup
Votes: 33,089
44. Parenthood (2010–2015)
TV-PG | 60 min | Comedy, Drama
The lives and tragedies of the Braverman family tree.
Creator: Jason Katims | Stars: Peter Krause, Lauren Graham, Dax Shepard, Monica Potter
Votes: 21,552
45. Exodus: Gods and Kings (2014)
PG-13 | 150 min | Action, Adventure, Drama
The defiant leader Moses rises up against the Egyptian Pharaoh Ramses, setting 600,000 slaves on a monumental journey of escape from Egypt and its terrifying cycle of deadly plagues.
Writers: Adam Cooper, Bill Collage, Jeffrey Caine, Steven Zaillian | Director: Ridley Scott | Stars: Christian Bale, Joel Edgerton, Ben Kingsley, Sigourney Weaver
Votes: 96,275
46. Nashville (2012– )
TV-PG | 43 min | Drama, Music
A fading country music star comes into conflict with a rising teen star.
Creator: Callie Khouri | Stars: Connie Britton, Clare Bowen, Hayden Panettiere, Eric Close
Votes: 14,841
47. The Godfather: Part II (1974)
R | 200 min | Crime, Drama
The early life and career of Vito Corleone in 1920s New York is portrayed while his son, Michael, expands and tightens his grip on his crime syndicate stretching from Lake Tahoe, Nevada to pre-revolution 1958 Cuba.
Writers: Francis Ford Coppola, Mario Puzo | Director: Francis Ford Coppola | Stars: Al Pacino, Robert De Niro, Robert Duvall, Diane Keaton
Votes: 682,904
48. Prisoners (2013)
R | 153 min | Crime, Drama, Mystery
When Keller Dover's daughter and her friend go missing, he takes matters into his own hands as the police pursue multiple leads and the pressure mounts. But just how far will this desperate father go to protect his family?
Writer: Aaron Guzikowski | Director: Denis Villeneuve | Stars: Hugh Jackman, Jake Gyllenhaal, Viola Davis, Melissa Leo
Votes: 305,438
49. Switched at Birth (2011– )
60 min | Drama, Family
Two teenage girls lives are turned upside down when they find out they were switched at birth.
Creator: Lizzy Weiss | Stars: Sean Berdy, Lucas Grabeel, Vanessa Marano, Katie Leclerc
Votes: 16,607
50. Blue Bloods (2010– )
TV-14 | 43 min | Crime, Drama
Revolves around a family of New York cops.
Creators: Mitchell Burgess, Robin Green | Stars: Donnie Wahlberg, Bridget Moynahan, Will Estes, Len Cariou
Votes: 18,015


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Copper Canyon Crazies
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Old Cranky Guy
Joined: Aug 2006
Location: Not Toronto therefore off the edge of the world
Oddometer: 265
Day 8 - Chinipas to Paraiso del Oso
There was quite the discussion at breakfast about our nighttime visitor. What started out as 1 guy who scared us back into our room ended up as 20 guys with guns and we went out and opened up a can of whoop ass on them by the time I was done telling the story. Somehow I don't think Ivan believed me.
We will never probably know the story as to why the guy was there but as mentioned eariler, nothing was touched so it was time to get going. The road immediately started climbing back into the surronding mountains as we followed a ridgeline to the top...
We stopped near one of the local mines to gather everyone up and to make sure we had our bearings correct...
Whenever you are near a mine there is a possibility of coming across a military checkpoint...
Remember to play in full-screen mode.
Taking pictures of the police or military is a no-no but I forgot I had the helmet camera recording at the time.
We were taking a short break at an interestion gathering everyone up when Don got this shot of our token hillbilly, Russ...
It was a realatively short day and we rolled into our hotel, Paraiso del Oso Lodge by mid-afternoon...
We had lots of spare times on our hands so we decided to go into town and do a little sightseeing to check out the Jesuit mission in Cerocahui. I was the only one to ride over as the rest of the group had already changed into tourist attire and rode over in the lodge owner's van. The mission was easy to find since it was in the town square and the lodge owner had given me good directions...
There was a little bit of activity in the square but acually the town was pretty quiet...
The rest of the group went for a walk to visit the Tarahumara Indian Girl's School. Chris and Phil had some candy to hand out to the girls. Fortunately Scott stayed back at the hotel or else the girls would have all ran inside to hide. Don took this photo and had previously been to the bar and was already a little "fuzzy" which might explain the fuzzy photo...
Meanwhile I had not really wanted to leave my bike & gear unattended so I headed back and stopped to take a photo of a indian couple walking back through the trees to their hut...
When I got off the bike back at the lodge I heard some yelling from the top of the cliff. At first I thought is was Scott and I was yelling back for him to jump but it was Barak that had decided to go for a climb...
Russ took this photo of yours truly relaxing before getting ready for dinner...
The owner is an American now living in Mexico so for dinner it was Steak & potatoes. Our bartender was an English girl (a POME - Prisoner of Mother England - as the Aussies would say) who was a licenced electrician back across the pond but had decided she wanted to see Mexico, saved up some pounds, quit her job and picked up an intern job at the lodge and was working her way around Mexico. Interesting girl to talk to.
After dinner, our guide Alphonso got a hold of a guitar and we had an inpromtu karaoke in the middle of the Copper Canyon region.
Here are the stats for the day...
Next Post - The final ride day
Confucius said: "No matter where you go . . . there you are."
For the Dirt - 08 WR450F
Rapid Transit - 06 FJR1300AE
yamafitter screwed with this post 11-26-2010 at
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The 2015-16, 2016-17, 2017-18 KYRC contract plan outlined in this document was developed in collaboration with the Early Intervention parent support group, parents involved in the Peer Support Partner program coordinated through the KYRC, and staff from LRC's Early Intervention Unit.
General Internship
For students who are studying in traditional fields, such as social work, psychology and education, we also offer internships for them to be able to gain practical, hands-on experience in service coordination/case management and other social work-related skills.
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If you're interested in a general internship with Lanterman send an inquiry to Lanterman's Human Resources Department. Get contact details