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Farmville Tips and Tricks That Work: Free Coins and More
In this guide I will tell you how you can use some tips and tricks for gaining experience levels quickly and making more money on FarmVille. FarmVille is a popular game on Facebook. Tips and Tricks have been tested and work.
CHECK OUT THE AMAZING UPDATED AND IMPROVED FARMVILLE TIPS AND TRICKS THAT WORK ARTICLE AT : http://www.articlevoid.com/gaming/internet-games/farmville-tips-and-tricks/
GO TO: http://www.articlevoid.com/gaming/internet-games/farmville-tips-and-tricks/ FOR THE UPDATED VERSION OF THIS ARTICLE! THANKS
Some say that FarmVille is a very good game application on Facebook, they are wrong……it is the BEST game application on Facebook and in this article I will tell you about a few things that can help you level much quicker than you thought you could.
FarmVille Experience Level Points Cheat That Works
While playing Farmville you will sometimes have the problem of having a lot of money but not enough land to plant anymore seeds in order to get experience points. This trick will also help you if you are very close to a higher experience level but have no plots available to plough for experience points. You need some money saved up in order to use this trick. So don't buy any decorations and spend Farmville coins on things that will not help you level up.
To get started, you will need 10 plots that are empty or that need to be ploughed. Just calculate how much money you will need when you really want to harvest, such as watermelon that take 4 days to harvest but sell for 348 FarmVille coins. You are not going to be planting the watermelons right away in these plots. You are going to need 1300 FarmVille coins so try and gather that much and do not spend any money on anything. You will also need 150 coins for ploughing those future watermelons plots.
To start this FarmVille trick that actually works, plough your 10 plots and then buy soybeans. It will cost 150 coins to plough and 150 coins to plant soybeans, but delete those plots using the shovel tool immediately after seeds have been planted.
You might ask yourself why you should delete FarmVille plots in order to gain experience level points, but ploughing and planting soybeans will gain you 30 points on those 10 plots. You can repeat this trick process, every time you do it, it will cost you 300 FarmVille coins. This is the cost for ploughing and planting. Your FarmVille experience will quickly rise and you will become a step closer to reaching your goals. After you have all the wanted experiences gained a new level, you can plant the exact seeds that you wanted to buy. You can even expedite this way by using your harvester, tractor, and seeder if you already have them.
Good work…My best wishes to you
the unlimited gifts trick does not work. when i click on accept, i cannot find those unlimited gifts in the farm gift boxs. i can just get the one that was sent by that person first
I've not tested recently but it should work. What browser are you doing it in?
mozilla firefox
Thanx nice tips
Unlimited gifts glitch not working (FF,IE Chrome)
Question – do you know what does the gold coin that occasionally appears on farm (see http://images.stanzapub.com/readers/2009/10/04/flyinggoldcoinsfarmville_1.jpg )
10x for tips
yes. great firefox
please! add me to do a perfect nieghtbor
god bless u!
Blueberries don't give you the most money.. where did you get that from
@gena: the gold coin is a reward for plowing (i don't know how many fields). I think it adds 100 coins.
The multiple gift glitch seems to have been repaired unfortunately… however, I have recently discovered another one.
When you receive a cow in your gift box, buy a cow shed and move the cow straight from the gift box to the shed. The cow will still be in your gift box to do again until your shed is full!!! I have filled 3 cow sheds in this way!! (20 cows in each shed – using just the one cow from gift box!)
This didn't work for me. I am using Firefox.
Unlimited gifts cheat does not work….they are blocked my farmville makers…..!!!! so dont try to use it…..it will not work whatever browser (IE/ Firefox/ Safari/ GC) you use……!!!!
Just to mention that Raspberries are far better now for experience if you have enough time to plant and harvest them immediately. Once you reach 3 stars and start getting experience for harvesting it turns into 1 exp per hour.
Unlimited Gifts trick does work I was able to check asIhave 2 farms…!! In Firefox….opening new windows ….maybe you used new tab?
Unlimited gifts does not work for me either…My daughter sent me 4 gifts and they showed as me receiving 4 famville gifts but when i accepted them it took me right into Farmville and no gift was in my giftbox…..
yep the gift thing doesnt work. sad but true. its a waste of time and the person who accepts them is taken to his/her farm with no gifts.
Very nice Tips …. really these are very common ways to ding this …. but we did not think of it yet… nice work dude…
Hi unlimited gifts works good. I have safari and no pb at all. I did it twice today…
What does the golden coin means. It sometimes happen when I harvest, there is a coin flying from the field.
Pls explain
Hi all,
I have problems adding neighbors. They are listed "pending" and impossible to go further. I can send them gifts and also help them but they don't appear as neighbors..
Can someone help ?
Please ?
Thank you for the cow trick Zabby!
Gifts tricks worked very good for a while and now stopped… someone knows why ?
Can someone help please ? I need answers… may be one of the farmers experienced these pb.
hi, the multiple gifts were probably discovered by zynga and has been corrected…i have pending gifts i got from multiple gifts, but couldn't accept them anymore…but if the gift was sent using regular send gift function, i am able to accept them…but hey, this is just my thoughts…
I found gold coin twice which sprung from the field during plow and noticed that each time it yieled 100 coins. Both coins were in the left side of my property………wonder if more can be unearthed ? Wonder what really triggered it too !
Unlimited gift cheat probably wont work anymore. Any Idea's why? They probably read this (or one of the countless others) and found it out. A shame indeed, but oh well.
Also the gold coin gives you 100 coins, and it is totally random.
Does the unlimited gift also works in google chrome?
OMG it works the unlimited gift one works !!
i just tried it with my friends account !!
I got four presents !!
Thankss A Lott !!!
Best Wishes !!
Juliee !!
OMG it works the unlimited gift one works !!
i just tried it with my friends account !!
I got four presents !!
Thankss A Lott !!!
Best Wishes !!
I FREQUENTLY HAVE PROBLEMS POSTING A REQUEST TO BE WRITTEN ON MY WALL I have updated often. It is very rare that the box allows typing.
How do you get more land or a bigger farm?
I would reccomend buying the ARTICHOKES for 70 coins, and then re-sell them for 204 coins each. Then, help out and fertilize ALL of your friends farms. Thats what I usually do and I'm on LEVEL 15 after only a few months.
I think the best for starters is Soybeans. Later on, go with the grapes – it makes 170profit/day. The artichokes takes 4 days to grow and you could harvest 4 other crops during that time.
Trust me, I starded on january 4th and now after 2 weeks I'm level 21!!!
@Matt and Sabine:
I've been playing two weeks and I'm level 21 as well.. lol!
unlimited gifts works with latest firefox for me..Great tips!! Cheers!!
If you get some money saved up, try this to gain experience….
The ribbon category "Fenced In" can be completed quickly. Clear a spot on your land and just start buying fence for 300 each. It does not matter where you put them, but have enough space to pile up a few hundred. Keep buying until you complete all ribbons, then sell them all. You will still have the blue ribbon, and the massive XP's that come with it. And yes, you will be down on the coins, but its worth it. Now try the same with the "Architect" category, buying Rest Tents for 1000 coin each. You will quickly have your blue ribbon, gathering the huge XP bonuses for each ribbon. The giant coin bonuses help offset the cost of the tents and fences.
as you level you unlock more and more crops the highest crop you have unlocked is generally the best for me its asparagus 16 hours buy for 220 coins sell for 357 coins and 2 xp
free gifts
i think the grapes bring the largest profit, because to plow and plant, it costs 100, and you're getting back 275 i think, so that has the largest profit margin. Just because something harvests in 4-6 hours, and you planting them 5 or 6 times in a day, does not mean that you'll make more coins; at the same time, you're ending up paying more every time for plowing as well, whereas for grapes, they take a day, and you only pay for plowing once, and over the course of a day, the bring in more profit.
All i want is like two villas. and im always so broke. this stuff takes to long. i want something where i can get it now.!
May I add you to my website
May I add you to my website
unlimited gifts DOES NOT WORK…
stupid game
cool guide man i'm farmville fan
the gift trick is amazing, thanks!
Yes you may.
Great site. I didn't even know about the gift trick. Thanks. Click my name and check out my site. Let me know how it is. I just started it
Hey Darkplanner, since you posted this… the Getting Unlimited Gifts was working beofre hand really well and now it's not working…thanks for makeing them aware of this and them fixing it..
Yeah, I might decide to make an updated one sometime in the near future.
then r great tricks but i already know them ……………….:)
if u have some more tricks then plz……tell me
u can add me. my id is email@example.com
Send me a gift please i'll send one back
i just found a legit website that is giving away free 310 fv cash in less than 5 minutes.check it out here http://bit.ly/zyngafvcash also Watch the beautiful video testimonials inside.its a great website indeed
i just found a legit website that is giving away free 310 fv cash in less than 5 minutes.check it out here http://bit.ly/zyngafvcash also Watch the beautiful video testimonials inside.its a great website indeed
A tip – save time and use a wall bonus collector like BonusBuddy http://www.facebook.com/bonusbuddyapp It's easy to use and works great!
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text:
August 20, 2012
Photo by Elaine Rackley
Betty Murphy, 88, talked baseball with former Atlanta Braves pitcher Tom Glavine at the second annual "Crusin' for a CURE" fundraiser Saturday. The event featured a car show, inflatables, and Glavine signing autographs.
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subset: commoncrawl


text:
Minneola faces bleak financial picture thanks to sewage plant
Column | Mar 2, 2011
Minneola faces bleak financial picture thanks to sewage plant
Column | Mar 2, 2011
Olympic hockey: Gold is the goal for U.S. men and women
Story | Sep 10, 2009
The slogan for Canada's aspiring Olympians at the Vancouver Winter Games is "Own the podium." Dave Ogrean, executive director of USA Hockey, said Thursday that his organization is using that as a jumping-off point for its own athletes, but with...
U.S. women's national hockey team named
Story | Aug 24, 2009
Angela Ruggiero, a former Simi Valley resident and three-time Olympic medalist, led the list of 23 players named to the U.S. women's national hockey team in preparation for the Winter Olympics next February at Vancouver. Ruggiero, a defenseman and...
U.S. women's Olympic hockey roster announced
Story | Dec 17, 2009
Defenseman Angela Ruggiero of Simi Valley was nominated to her fourth Olympic team Thursday, when USA Hockey announced the roster for the upcoming Vancouver Winter Games. Ruggiero shares that distinction with Jenny Potter, formerly Jenny Schmidgall. Two...


text:
System and method for providing a sample preparing arrangement (10) submergible in a liquid medium that includes a carrier structure (11) having at least one cavity (12) therein and that is in communication with an arrangement (13) for controllable generation of a magnetic field through influence of a control signal. The sample preparing arrangement includes a magnetic covering structure (14) for covering and/or uncovering the cavity in operative interaction with the magnetic field.
1. A sample preparing arrangement comprising:
a carrier structure;
at least one cavity in said carrier structure;
a magnetic covering structure for covering/uncovering said cavity;
a magnetic field generating element comprising an electric circuit proximal to the cavity for controllably generating a magnetic field, wherein the magnetic field regulates the positioning of the covering structure;
a control signal for regulating the magnetic field generated by the element and for regulating the covering/uncovering of the cavity; and
means for detecting the presence of the magnetic covering structure when it is covering the cavity, wherein the means for detecting the presence of the magnetic covering structure is selected from the group consisting of: a control circuit for detecting a chanae in the inductance of the electric circuit of the magnetic field generating element caused by the presence of the magnetic covering structure, an electromagnetic radiation detector inside the cavity, and a capacitance detector near the cavity.
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11. The arrangement as recited in 1, wherein the magnetic covering structure is a magnetic bead that is directed onto the cavity using external magnets that create magnetic fields counteracting a field created by the magnetic field generating element.
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24. A method for preparing samples with a sample preparing arrangement, the method comprising:
providing the sample preparing arrangement of
providing the control signal to the magnetic field generated by the element to cover or uncover the cavity; and
detecting the presence or absence of the magnetic covering structure using the means for detecting.
25. The method as recited in
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This application is a continuation patent application of International Application No. PCT/SE01/01798 filed 23 Aug. 2001 which was published in English pursuant to Article 21(2) of the Patent Cooperation Treaty, and which claims priority to Swedish Application No. 0002990-0 filed 23 Aug. 2000 and to U.S. Provisional Application No. 60/228,015 filed 24 Aug. 2000. Said applications are expressly incorporated herein by reference in their entireties.
1. Technical Field of the Invention
The present invention relates to a method and arrangement for preparing samples submergible in a liquid medium.
2. Background of the Invention
When considering a living organism, for example a human being, an animal or even a plant, a basic subunit of such organisms is a cell. One way to categorize such cells is by the functions they are aimed to perform in an organism; e.g.: epithelial cells, (skin) muscle cells, neural cells and the like. All these cells communicate with the surrounding world via complex mechanisms, which usually involve many different complex molecules called proteins, some of which are embedded into a cell wall. Cells live and die very much like the whole organism; they also grow, divide, and the like. In all these functions, many different parts/components of a given cell take part. These components can be proteins, enzymes (acting as catalysts for certain reactions occurring in the cell) as well as DNAs, RNAs, tRNAs, and the like. Thus, there exists an enormous number of processes (mutations) occurring in an organism per unit time, and also in each cell of the organism. Some of these mutations are important for the cell-well being, but others are dangerous, for instance, cancerous mutations.
Therefore it is very important to be able to foresee, at least partly, the behavior of a cell, to map out the reactions that occur, and their products (usually creation of new cells DNAs and/or proteins). It is equally important to be able to cure maligneous events in the body, which can arise by either invasion of other organisms (viruses or bacterium), or by processes caused within the body itself (autoimmune reactions), or by outside environmental factors such as stress.
In all of these processes, the number of out coming events is immense. Take for example, DNA strands. There are millions upon millions of different DNAs that contribute to production of even larger numbers of proteins whose function and chemistry is far more complex than that of the DNAs themselves.
It would take many lifetimes to establish the structure of even a few thousands of the DNA molecules, not to mention the proteins and/or their functions, or related drugs. Therefore, one needs fast techniques that enable the acquisition of information in parallel, and effective means of storage and handling of such information.
During the past decades, a search to develop such methods has been started. The common name for such techniques has been coined as high throughput screening (HTS). The idea is to prepare, in parallel, fewer samples so as not to use large amounts of expensive and rare chemicals but, to make as many as are feasible, as is possible. The easiest and the most logical (from the information handling point of view o) way is to arrange such complex samples in a matrix. Taken from the semiconductor industry, these matrices are often referred to as "chips".
One example of such preparation is given in
The number of elements (spots) in a matrix varies depending on the preparation method, but usually does not exceed 10,000; although, matrices as large as including 1,000,000 sites have been reported. The outcome of each single "experiment," therefore, gives at best 10,000 results. In reality this number is much lower (around 20% of best results) due to the very poor quality of even the best matrices produced to date.
FR 2,781,886 concerns fabrication of a microsystem with multiple points for chemical or biological analysis consisting of a structure provided with micro-cups. Each micro-cup is designed to receive a reagent coupled with a conductor polymer. Each micro-cup has a receiving electrode whereon, is fixed, the reagent via of the polymer conductor, with which it is coupled. Each micro-cup also has a counter-electrode arranged so as to apply, in a volume of the micro-cup, an electric field between its counter-electrode and its receiving electrode. The structure has means for simultaneously connecting all the receiving electrodes to a first electric potential and means for simultaneously connecting all the counter-electrodes to a second electric potential for generating said electric field.
U.S. Pat. No. 5,874,219 discloses methods for concurrently processing multiple biological chip assays by providing a biological chip plate comprising a plurality of test wells, each test well having a biological chip having a molecular probe array. Samples are introduced into the test wells; subjecting the biological chip plate to manipulation by a fluid handling device that automatically performs steps to carry out reactions between target molecules in the samples and probes. The biological chip plate is subjected to a biological chip plate reader that interrogates the probe arrays to detect any reactions between target molecules and probes.
U.S. Pat. No. 5,755,942 describes a system for processing a plurality of tests or syntheses in parallel that include a sample channel for moving samples into a micro-laboratory array of a plurality of wells connected by one or more channels for the testing or synthesis of samples. A station is provided for housing the array and an optical system comprising at least one light source and at least one light detector for measuring the samples in the array. A means is provided for electrically connecting the array to an apparatus capable of monitoring and controlling the flow of fluids into the array. Samples are loaded from a common loading channel into the array, and the samples are processed in the wells. Measurements are also taken by the optical system of the samples. The system uses a magnetic valve for opening and closing a channel for micro-fluids.
One object of the invention is to present an arrangement which improves the "one by one" experimentation by providing a controllable space.
The technique allows a relatively rapid screening of new chemicals to be used as drugs, both with regard to their function and, importantly, with regard to the determination of the side effects that a given drug might exert. The technique can also be used in many other applications such as genome determination, proteomics and others.
The invention can be used to prepare a ready-to-use-product, which is impossible to modify, but to allow a user for possibility to prepare his or her own "experiment". Thus, one object of the invention is to provide an easy-to-handle platform, which can be used repeatedly and can be prepared in-house. Consequently, the invention is not limited to the surface deposits as is the devices described above (see also
In an arrangement configured according to the invention, it is relatively easy to change both the dimensions and the number of the wells. Also, the simplicity of the design will allow integrating the reaction product detection system, on-chip, and perhaps also the facility for multi-well deposition of the active substance.
Another object of the invention is to describe how the detection limits for the events under study can be improved using techniques similar to those used for chip production.
These objects are achieved by the initially mentioned arrangement, which includes a section provided with a device for controllable generation of a magnetic field through influence of a control signal. The magnetic field is generated to trap at least part of the samples. Preferably, the device is a coil or a magnetically active material and it is made of an electrically conducting material, preferably aluminum. In each device, a current of different strength is applied through a conductor whereby the current amplitude and the number of windings in the coil are proportional to the strength of the magnetic field.
According to a first aspect of the invention, the arrangement includes a cavity provided in a substrate and a lid for closing the cavity. Preferably, the lid is a magnetic bead. The bead is directed onto a cavity using external magnets that create magnetic fields counteracting the field created by the material deposited around each cavity. Each cavity is surrounded by a device, which directs the lid using external magnets that create magnetic fields counteracting the field created by material deposited around each cavity. The cavities are etched in a silicon surface and the lid is provided as a large magnetic particle in the liquid. The particle is attracted to a predetermined cavity when the coil of the cavity is energized by electric current to produce magnetic field of spatial attraction. Before sealing off the cavity, smaller magnetic particles are attracted into the cavity. The sample is a magnetic particle covered with appropriate chemical(s).
In one embodiment, the arrangement includes means for detection of presence of a magnetic capping lid capping a cavity. In one embodiment, the capping is detected by detecting the change in inductance in the control circuit, which produces the attractive magnetic field whereby the bead acts like a magnetic yoke in a transformer, increasing the inductance. In another embodiment, the capping is detected through decrease of electromagnetic radiation to a detector inside the cavity or by changes of capacitance between electrodes inside the cavity or near a cavity rim.
The arrangement may also include means for detection of changes of inductance when a magnetic particle passes through the opening into or out of a cavity. The indication is determined using the direction of externally controlled magnetic field, either by changing the direction of the electric current flowing through a coil or flipping an external magnetic. Preferably, the particle contains particular molecular coating, which reacts with the liquid in that cavity or with the coating adsorbed on the walls of the cavity.
The substrate can be made of silicon, Si, or of Si-compound, such as Si-oxide Si-nitride or Si-carbide, or combinations thereof, or a suitable polymer, such as polyethylene, polyethylene glycol, polyethylene oxide, fluorine containing a polymer (PTFE BTeflon), or silicon containing a polymer.
The invention also relates to a method of preparing samples, by means of an arrangement submergible in a liquid medium. The arrangement includes a section provided with a device for generation of a magnetic field. The method includes the steps of connecting a signal to the device and generating a magnetic field to trap at least part of the samples. Each device is applied a current of different strength. The arrangement is provided by a cavity in a substrate.
According to this method, it is possible to detect the presence of a magnetic capping lid capping a cavity. The capping is determined by detecting the change in inductance in the control circuit which produces the attractive magnetic field. In this way, the bead acts like a magnetic yoke in a transformer, increasing the inductance. The capping may also be determined through decrease of electromagnetic radiation to a detector inside the cavity or by changes of capacitance between electrodes inside the cavity or near the cavity rim. According to the method, it is possible to detect changes of inductance when a magnetic particle passes through the opening into, or out of a cavity, thereby determining the indication using the direction of externally controlled magnetic field. This is accomplished either by changing the direction of the electric current flowing through a coil, or flipping an external magnetic.
According to the method, given a known number of samples in each cavity and a density of respective coatings, quantitative data on the number of reactions between the coating on a wall of the cavity and the coating on a small sample is obtained by counting the number of samples.
In the following, the invention will be further described in a non-limiting way with reference to the accompanying drawings, in which:
A basic idea of the present invention is to create an enclosure or a crater (a well), provided with a lid, and that can be opened and closed by the "lid". A user can control the lid, and the device is intended to be submerged in a liquid medium. By operating the lid, the enclosed volume becomes separated from the surroundings. That means, the liquid stored in the crater, including particles suspended therein, and/or material that adheres to the crater's inner surface, are not affected by subsequent changes that occur in the surroundings while the lid is closed. These changes might be a different chemical composition of the liquid, light shining on the crater chip, or other solids in the surrounding liquid. The lid may or may not be completely liquid-tight, but mixing of the liquid outside a crater with the liquid contained inside a well will be dramatically slowed. Hence, solids and liquids will be well separated between inside and outside by the lid.
By making many craters 12, all with individually controlled lids 14, different types of mixing of solids dispensed in a liquid and/or liquids can be achieved at the same time. As different liquids/solids are introduced to the outside of the craters, only user-selected craters with open lids will be reached for the mixing by the liquids/solids external to the closed craters.
The dimensions and the shapes of each crater 12 can of course vary within a large interval both with respect to its diameter and depth. The craters can have circular cross-section, for example being about 50 μm deep with diameters of approximately 100 μm. It is relatively easy to produce craters with dimensions ranging from a few μm and larger, and with depths ranging from a few μm and up to several hundreds of μm, in, for example, square shapes.
The material of the substrate can be silicon and the manufacturing process may include micro-machining, similar to the process of making microprocessors or memories chips. A device configured according to the invention may contain from several hundreds of craters on a single piece of silicon, establishing a so-called chip. Of course, tens of thousands of craters on commercial units can be arranged.
Preferably, the lid is a micro-bead introduced in a liquid. The lid-actuation mechanism that is used for the closing and the opening each of the craters can include using switchable magnetic fields that influence the motion of the introduced beads. The magnetic fields are created using the coils 13 deposited around each crater.
The coils 13 surrounding each of the craters are made of an electrically conducting material. In a preferred embodiment, the conductor is made of aluminum, Al, but any electrical conductor can be used. Preferably, each coil is accessible through electrically conducting leads so that a current of different strength can be applied separately to each coil. The current amplitude and the number of windings in the coil are proportional to the strength of the magnetic field, which can thus be varied. Clearly, it is possible to change the number of windings in the coils surrounding each crater as well as their width and thickness within a broad range of dimensions. Preferably, but not exclusively, coils can have from 2 to 10 windings.
In an alternative embodiment, instead of the coils 13, the control means can take the form of a magnetically active material surrounding each crater and which directs the beads using external magnets that will create magnetic fields counteracting the field created by the material deposited around each crater 12.
Preferably, the craters are etched in the silicon surface and the lid is provided by a large magnetic particle 14 in the liquid. Thus, particle 14 can be attracted to the crater of choice when the coil of this crater is energized by electric current to produce magnetic fields for spatial attraction. Before sealing off the crater of choice, however, it is also possible to attract smaller magnetic particles into the crater. To attract the smaller magnetic particles 15 to the crater, the coil is energized by an electric current. When the coil is energized, a magnetic field is established. This field will attract the magnetic particle 15 from the liquid. These smaller particles have higher mobility in the liquid compared to the mobility of larger particles and will thus reach crater faster then the larger lids. The large lid-particle will cap the crater at a later stage. Preferably, as large particles commercially available magnetic particles such as ferromagnetic or super-paramagnetic having about 100 micrometers in size can be used, while the size of the smaller particles is much smaller than the crater's size. There are other dimensions and particle types on the market and the invention is applicable a broad range of particle sizes, shapes and materials.
To open a closed crater, a repelling field is generated either externally or by inverting the direction of the current flowing through the coil. It is also possible to terminate the current through the coil, whereby the particle may be released due to shear force from the flowing liquid or due to gravitational forces if the craters are positioned "upside down."
The simple actuation of the crater lid using a current controlled magnetic field(s) and the large number of craters on a chip makes it necessary that the chip be operated automatically through a controlling arrangement. The chip is preferably provided with an interface device that establishes electrical connection with the chip and provides the handling of the surrounding fluid with the beads and chemicals. After use, the chip may be removed for cleaning and reuse or disposal. The interface device will be connected to a computer equipped with suitable software to control the sequence of operations on the craters and the liquid handling system. The software will also provide an interface for the user to establish the process sequence and to plan the states of the crater lids in each sequence.
Detection of a magnetic capping bead can also be done. It is important to obtain feedback on which craters are capped. The presence of a magnetic capping bead, in place over a crater, can be detected by the change in inductance in the electric circuit, which produces the attractive magnetic field. The bead acts like a magnetic yoke in a transformer, increasing the inductance. A resonant, or other circuit can then detect this inductance change.
The presence of the capping bead can be detected by various other schemes such as a decrease of electromagnetic radiation experienced by a detector (112) inside the crater, or by changes of capacitance between electrodes inside the crater or near the crater rim.
Another possible application along similar lines is the detection of changes of inductance when a small magnetic sphere passes through the opening into a well. Using this arrangement, according to the invention, it is possible to determine whether a sphere is entering the well, or if it is leaving the well. This is determined using the direction of an externally controlled magnetic field either by changing the direction of the electric current flowing through a coil or flipping an external magnetic field creating device by other means. Such a sphere may contain particular molecular coating, which will react with the liquid in that well or with the coating adsorbed on the walls of the crater. Given that the number of spheres is known in each well, as is the density of the respective coatings, quantitative data on the number of reactions between the coating on the wall and the coating on a small bead can be obtained by simply counting the spheres.
In the following non-limiting examples are given for simplifying the understanding of the invention. According to a first example, liquid A containing magnetic beads is introduced. User selected craters 12 are energized, and hence capped. The remaining beads are flushed away with a cleaning liquid. Now liquid B is introduced, containing small (much smaller than the capping beads) particles, called X, made of a material interesting to the user. Only uncapped craters will accept X. Then, more magnetic beads are introduced and selected craters are capped, trapping X. Cleaning liquid is used to flush all excess away. A liquid containing chemical reagent Y can then be introduced and some craters are opened. X and Y are allowed to mix and react, but only in the user-selected areas. This reaction can be followed using sensing techniques that can easily be incorporated into the system, for example using optical techniques. Other possible novel detection techniques that are easily incorporated into the present embodiment are described below.
In a second example, a substance is attached to the craters inner surface. In a repeating sequence, some craters are closed by the beads and the others are exposed to a reactive chemical A. After the reaction, the chemical is flushed and some craters are exposed to another chemical B. As a result, there will be craters that have been exposed to A and B, some to A, some to B, and some to neither. This process can be repeated with many chemicals producing very large numbers of differently modified substances residing in different locations (craters) of choice. With a sequence of 10 different chemicals, for example, more than 1000 different combinations are obtained. In particular, this could be used to synthesize DNA strands or (using appropriate well-known techniques) to investigate the function(s) of different proteins.
Yet another application of the present invention is to lock the cells in the wells filled with different chemicals and monitor the reaction of cells (cell proliferation, differentiation, spreading or others) to these chemistries. This would enable, for example, a fast, high throughput screening of drugs.
The arrangement may also be used separately, one-by-one, for example to deliver a certain chemical, or chemicals, locally at a certain place or places in a reaction vessel, and monitor reaction products locally, or to deliver a drug inside a body.
Another field of possible applications of the device has been triggered by something generally referred to as a "low throughput screening" (LTS). LTS is often used when the amount of required information is smaller, but the researcher wants to additionally obtain some quantitative information about concentrations of analyses or number of reactions that occur during certain time at certain amounts of reagents. The idea behind LTS has much in common with another timely idea often used today and referred to as an "electronic tongue." Electronic tongue is a device that enables one to determine components in a liquid. These components can then be associated with certain tastes (sweet, sour, salt, etc. or combinations thereof). To determine the content of simple liquids in a liquid mixture, for example % of sugar dissolved in a cup of tea along with the amount of tea used to prepare this cup, and even perhaps different tea blends used. To acquire knowledge about all these requires performing several experiments with constituents that react differently to different tea blends and to different amounts of tea from each blend that has been used, as well as to the amounts of sugar being dissolved in this tea. All these can be made by LTS methods using the presently disclosed equipment, and choosing appropriate reagents different for each crater and letting these first react with "standard" samples ("teaching the tongue" to recognize certain non-mixed liquids) and later exposing these samples to mixtures of different tea blends, with or without sugar. Appropriate data processing from the outcome, compared with the results obtained on standard samples, enables one often to obtain information about tea blends used and the amount of sugar dissolved.
The device is not limited to spheres or coils for creation of magnetic fields that direct beads, nor is it limited to the use of beads. Other shapes can be used. Finally, the present invention is not limited to the use of silicon technology to fabricate the crater matrices; other materials can be used for this purpose.
Additional, non-limiting examples are provide below of different crater preparation techniques, and materials of use paired with its utilization. A general concept behind these examples is to manipulate small particles in order to bring them to a chosen place on the surface of the substrate using magnetic field(s) as a driving force for particle manipulation. The surface of the substrate may or may not be patterned in a particular manner. When the substrate is patterned and the pattern consists of craters, some particles are used preferably as caps or lids to close each crater as earlier described. When the substrate is left without a pattern or patterned in a different manner (see below for an example), the particles can be used mainly as a way to enhance sensitivity of detection of the processes taking place in the device.
The magnetic force to manipulate the particles can be created using coils as described above, but it can also be created using externally applied magnets. In the former case, the field strength (and thus the magnitude of the force) is determined primarily by the number of windings in the coil and the magnitude of the electric current passed therethrough. In the latter case it is possible to control the magnitude of the magnetic force by appropriate choice of magnet position and strength.
The substrate may be made of silicon (described above), Si, or of Si-compound, e.g. Si-oxide Si-nitride or Si-carbide, or combinations thereof. It may also consist of thin self-supporting Si, or of a Si-compound, with another film of suitable thickness (for example few micrometers), such as ZnO, evaporated onto its surface. This additional film is needed if the device is to work as an acoustic wave device for detection.
The substrate may also be fabricated using other material than silicon. For example, a suitable polymer, e.g. polyethylene, polyethylene glycol, polyethylene oxide, fluorine containing a polymer (PTFE BTeflon), or silicon containing a polymer may be used as a substrate material.
When patterning, the different substrate techniques may be used depending on the substrate material and the pattern. Thus, Si and Si-compounds are suitably patterned applying techniques known from semiconductor fabrication. When patterning polymers, one can use known techniques like polymer stamping or moulding.
It is also possible to supply iron or any other magnetically active material as yoke somewhere inside the crater coil geometry to concentrate the magnetic field to that region, when a coil is activated. This can be inside, below, or around the actual crater. The idea is to build the crater coils in several layers around a crater to increase the magnetic field strength. The crater coils can be used to expel all magnetic material from inside selected craters. Magnetic material can be attracted to the inside of selected craters. Smaller magnetic particles will get attracted to the center of a coil but will sink to the bottom of the crater due to the force of gravity.
It is also possible, in advance, to prepare the floor or the walls of all the craters as a bio/chem friendly surface, while the rest of the chip surface stays inert, or vice versa. In this way, both the capping beads and the type of surface will determine where a new bio/chem substance will react or be active.
Moreover, capping beads can be used as a chemically/biologically active surface, while the rest of the chip surface is not, or vice versa. By supplying magnetic capping beads, which have been bio/chem prepared in advance, to the chip, a particular bead (surface) will be attracted to a pre-determined site. Hence, the user can, by repeatedly applying different beads and washing off unattached beads, cover the chip surface with different and well-defined surfaces. Thus, it is possible to trigger a chem./bio reaction/activity inside a crater by attracting a capping bead with the right bio/chem active surface.
The invention is not limited the shown embodiments, but can be varied in a number of ways without departing from the scope of the appended claims and the arrangement and the method can be implemented in various ways depending on application, functional units, needs and similar requirements.
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Baseball Preview: Team By Team
Narrows League
Central Kitsap Cougars
Coach: Bill Baxter, 20th season
Last year: 5-13 (5-9 league)
Top returners: Riley Paschal (C, jr.), Alec Anderson (INF/OF, sr.), Eric Braun (P/1B, sr.); Chanlin Morgan (OF, sr.); Hunter Culp (1B, sr.); Chandler Manson (SS, jr.); Bryson Duarte (3B/P, jr.), Brion Anduze (3B/1B/P, jr.), Nick Barbero (P/OF, jr.).
Top newcomers: Ben Kunkle (OF, sr.), Colby Cain (OF, sr.), Drey McInnis (INF, jr.).
South Kitsap Wolves
Coach: Marcus Logue, first year
Last year: 12-6 (10-4 league)
Top returners: Kellen Traxel (P, sr.), Tom Simpson (OF/P, sr.), Kevin Whatley (SS, sr.), Michael Wood (P, sr.), Cody Wolfe (OF/2B, sr.), Jesse Moore (3B/1B, sr.), Alec Morrison (2B, sr.), Nic Stoner (3B, sr.), Josh Johnston (P, sr.), Logan Knowles (2B/P, jr.), Dylan Garcia (OF, jr.), Tyler Ludlow (C/1B/DH, jr.), Panner Paulson (C, jr.), Tyler Pinkerton (1B/C/DH, jr.).
Top newcomers: Bryce Broome (OF, sr.), Conner Sharp (P, jr.).
Narrows League Outlook
This is the first head varsity job for Logue, a Port Townsend grad who said he inherited some "real baseball savvy kids who are hard workers and fun to work with." UW signee Traxel, Wood, a top reliever a year ago, and Simpson give the Wolves three strong pitchers; Moore and Whatley can bash, and Logue said Wolfe and a trio of juniors — Pinkerton, Paulson and Ludlow — all looked capable of hitting in the middle of the order. ... Central's Baxter, the dean of prep baseball coaches in the area, thinks the Cougars will be in the hunt for a postseason berth. "We've got some kids who can play. It'll come down to pitching and hitting. Defensively, I think we'll be solid," he said. Pitchers Braun, Anduze, and Barbaro need to keep the Cougs in games. Anduze is the top returning hitter. Manson, and McInnes, a transfer from Bremerton, are solid up the middle, as is Anderson, who has started at second and outfield in the past. Paschal started as a sophomore and has the tools to be one of the league's best catchers. ... Olympia returns four first-team All-Narrows selections, and should be the team to beat. ... Gig Harbor's Nick Gagliardi is back after being named pitcher of the year as a junior.
Metro League
Bainbridge Spartans
Coach: David Smart, third year
Last year: 7-11 (7-9 Metro)
Top returners: Will Houser (P/INF, sr.), Spencer Hogger (3B, sr.), Zander Silverman (SS, sr.), Dylan Vchulek (CF, jr.), Drew Miller (OF, sr.), Ben Kussie (INF, jr.).
Top newcomers: Sam Tiffany (C, jr.), Tyler Riley (P, sr.), Drew Dierickx (2B, jr.), Brett Green (P/UTL, jr.).
Metro League Outlook
If the Spartans can get into the playoffs, and they just missed the last two years, Smart likes their chances with pitchers Houser (already committed to Cal Lutheran), Green and Riley. Catcher Paimon Jaberi tore his ACL in football and won't be able to play his senior year. "That's a big loss. He's a fantastic leader and a great kid," Smart said. ... Metro power O'Dea has won three state titles (2005, 2009, 2010) in the last eight years and, along with Eastside Catholic, is the team to beat. Pierre LeDorze, who lives on Bainbridge Island, and Connor O'Brien, son of former West Bremerton athlete and ex-Seahawk Mike O'Brien, play for the Irish.
Olympic League
Bremerton Knights
Coach: Steve Schorzman, second year
Last year: 9-8 (9-7 league)
Top returners: Eli Fultz (P/OF, sr.), Matt Noll (Utl/P, sr.), Beau West (P/1B, jr.), Nick Torres (2B, jr.), Ben Merrill (3B/P, sr.), Theo Robinson (CF/P, sr.), Casey Winderl (OF/P, jr.), Zach Zurbrugg (C, jr.).
Top newcomers: Anthony Amicangelo (SS, fr.), Zane Zurbrugg (SS, fr.), Taylor Sayler (3B/P, so.), Connor Mahoney (SS/P/C, so.).
Kingston Buccaneers
Coach: Jeff Tapp, second year
Last year: 15-9 (9-7 league; state participant)
Top returners: Beau Rabedeaux (SS, jr.), Ethan Syverson (2B/3B/P, sr.), Ben McMullen (UTL, sr.), Jackson Suter (1B, sr.), Alex Vandegrift (P/OF, sr.), Nathan Carlton (OF/P, jr.), KT Deam (2B, sr.).
Top newcomers: Auggie Rodriguez (C, jr.), Tyler Shuey (3B/P, jr.), Carter Draper (1B/P, jr.), Miles Sustad (P/OF, so.).
Klahowya Eagles
Coach: Dan Zuber, third year
Last year: 7-9 (7-9 league)
Top returners: Jacob Burton (2B/SS, sr.), Drew Fagan (OF/P, sr.), Dylan Keiffer (OF/P, sr.), Jacob Gotchall (3B/P, so.), Kendall Kitts (1B/3B/P, so.); Grady Bayshore (OF, sr.); Steve Harstad (OF, sr.).
Top newcomers: Ryan Gotchall (C, so.), Matthew Page (C, so.); Conner Schnuit (1B, so.), Tanner Zuber (2B/SS, so.).
North Kitsap Vikings
Coach: Jeff Weible, eighth year
Last year: 17-9 (12-4, league champs, state participant)
Top returners: Shane Crowell (INF/P, sr.), AJ Millyard (SS/P, sr.), Matt Fischer (2B, sr.), Will Ekin (OF, jr.), Kyler Gracey (OF/P, sr.), Brian Bratlien (3B, sr.).
Top newcomers: Andrew Hecker (CF/P, so.), Adam Lemmon (1B, jr.); Max Teising (INF, jr.), James Demile (C, jr.), Connor Green (C, so.), TJ Jensen (INF, so.), Ben Tamm (P/DH, sr.), Sheldon Parris (INF/P, jr.), Tyler Bryne (P/1B, jr.), Grant Loveless (C, sr.), Kole Millyard (P/INF, fr.).
North Mason Bulldogs
Coach: Bill Geyer, third year
Last year: 4-15 (3-13 league)
Top returners: Riley McKean (2B/P, sr.), Jordan Young (SS/2B/P, sr.), Chase Schoonhoven (C, sr.), Sam Sandell (1B/P, jr.), Morgan Grewell (OF, jr.); Hunter Hohmann (SS/2B/P, so.), Daniel Burggraaf (SS/3B/P, so.), Evan Daley (OF/P, sr.), Chris Stevens (OF, sr.), Andrew Burggraaf (OF/P, sr.), Ty Grewell (OF, so.), Justin Hill (3B, jr.).
Top newcomers: Brody Stromberg (OF, sr.), Manny Long (OF, fr.), Levi Rouse (INF, sr.).
Olympic Trojans
Coach: Nate Andrews, 13th year
Last year: 13-9 (9-7 league)
Top returners: Shane Matheny (UTL, jr.), Trevor Ward (SS, sr.), Tayler Huddleston (UTL, sr.), Noel Torres (OF, sr.), Evan Elevado (OF, sr.), DJ Wojeck (C, sr.), Erik Turnquist (2B/P, so.).
Top newcomers: Marcus Vanshur (OF, sr.), Kevin Beamish (UTL, sr.), Kipp Cartharius (UTL, jr.), Chuck Vetter (P, jr.).
Port Angeles Roughriders
Coach: Chad Waggoner, first year
Last year: 12-10 (11-5 league)
Top returners: Marcus Konopaski (C, sr.), Wesley Giddings (OF/P, sr.), Michael Konopaski (2B/P, sr.), Brian DeFrang (OF, sr.), Chase Jangula (3B, sr.), Kevin Herzog (OF, sr.), Coleman Tomasson (INF, sr.).
Top newcomers: Brady Konopaski (SS, jr.), Larson Chapman (1B, jr.).
Port Townsend Redskins
Coach: Tom Webster, first year (was an assistant last year and head coach prior)
Last year: 4-15 (3-13 league)
Top returners: Devon Courtney (C/CF, sr.), Dillon Ralls (1B, sr.), Cody Russell (INF/P, jr.), Jacob King (OF/P, jr.), James Delagarza (INF/OF/P, jr.), Harry Goodrich (INF/P, jr.), Emmett Davis (C/UTL, sr.), Sean Dwyer (INF/P, so.),
Top newcomers: Jacob Rawls (INF/OF, fr.), Joe Hoffmann (INF, fr.).
Sequim Wolves
Coach: Dave Ditlefsen, eighth year
Last year: 12-13 overall (9-7 league, reached district)
Top returners: Nick Johnston (P/1B, jr.), Brett Wright (C, jr.), Fred Serrano (3B, sr.).
Top newcomers: Nigel Christian (P/SS, fr.), Austin Clement (P, sr.), Cameron Harrison (SS/2B, sr.), Tanner Rhodefer (P/OF, so.).
Olympic League Outlook
North Kitsap is favored to win its fifth straight league title in a year that figures to be pretty competitive. North Mason, for instance, was picked to finish seventh in the nine-team league, and the Bulldogs return its entire pitching staff and all of its starting position players. ... In addition to experienced and talented returners such as Shane Crowell, A.J. Milyard, Matt Fischer and Kyler Gracey, North Kitsap will reload with quality players who sat on the varsity bench or played junior varsity ball a year ago. Don't sleep on senior pitcher Ben Tamm, a home-schooled athlete. Ben Tamm? "He probably should have DH'd last year and pitched for us but we didn't have any room for him as a starter," Weible said. "He just dominated the jayvees and had a great summer. He's been unhittable in our scrimmages, better than Shane (Crowell) and AJ (Milyard). ... I wouldn't be surprised if he's in the MVP picture at the end of the year." ... Bremerton coach Steve Schorzman has turned the Knights into a contender in two years. "With Eli (Fultz) and Matt Noll, I can't not like our chances," he said, talking about the ace of his staff and his speedy senior. "If we hit, we'll do well. We're going to play defense and our pitching will be good. We are blessed with a lot of arms. If one doesn't work, we'll move on to the next one." ... Olympic returns six starters, including Shane Matheny — arguably the best player in the league — and will be looking to qualify for the postseason for the seventh straight season. Coach Nate Andrews said he's got some good young talent competing for playing time. ... Kingston has made it to regionals five years in a row and despite being younger than usual, coach Jeff Tapps said the Bucs will compete with anybody. "Our goal is to always win the whole thing," he said. "We want to play hard every day, and do what we can to be the best we can be." Beau Rabadeaux is a good one at shortstop. ... Klahowya coach Dan Zuber said the Eagles, led by Dylan Keiffer and Jacob Burton, are talented enough to challenge for a playoff spot. The Eagles haven't hosted a home game in two seasons and that's a big disadvantage ... North Mason, as mentioned above, has plenty of reasons to be optimistic, including pitchers Riley McKean and Jordan Young. "We lost eight one-run games last year," said coach Bill Geyer. "If we can turn that around ..." ... Port Angeles' first-year coach Chad Waggoner played at Sequim and was the pitching coach at Lower Columbia College and also with the Kitsap BlueJackets last summer. The goal is to reach the playoffs, and he's got three Konopaski brothers (catcher Marcus, a senior; second baseman/pitcher Michael, a senior, and Brady, a junior shortstop), he can pencil into the lineup. Sequim returns just three players, and coach Dave Ditlefsen said, "This is definitely a rebuilding year." Port Townsend's best player, Kyle Kelly, moved with his father, Pat Kelly, who was the Redskins' head coach. The cupboard's not bare, but being a 1A school, PT doesn't have the depth that the bigger 2A schools have.
Nisqually League
Chimacum Cowboys
Coach: Jim Dunn, third year
Last year: 19-1 (12-0 league; lost at regionals)
Top returners: Derek Ajax (UTL/P, sr.), Alex Morris (C/P, jr.). Miles Hundley (1B/P, so.), Casey Settje (3B/P, sr.).
Top newcomers: Seth Hamm (DH/1B, sr.); Coby Schrieder (SS/P, sr.), Colton Shaw (OF/P, so.), Drew Yackluc (OF, so.).
Nisqually League Outlook
Mike Nordberg sustained a concussion in football and isn't available this spring. The senior would have been the ace of the staff. "Hopefully this is not a rebuilding year," Dunn said of his relatively young team. "Hopefully it's just a retooling year." The Cowboys have been among the elite Class 1A programs in the state, posting a 77-10 mark over the last four years. The Cowboys were third at the 2009 Class 1A tournament, second in 2010 and first in 2011. Chimacum was 19-0 before losing 1-0 to Kalama in the regional finals a year ago.
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Gear Review
Not properly sized for I-Phone
I really like the product. I really do. The ziplock seal is heavy duty...the plastic is durable, but allows you to use a device through it (i.e. you can type on your iphone while it's in the bag).
The Small (4x6) is supposed to be iPhone sized, but it isn't. It's about 1/4-1/2" too tight to fit comfortably. Once the phone is in, you're fine...but it's really hard to get the phone in or out. Add the sticky plastic of the case to the situation, and it's just not a well-functioning piece of gear.
The next size up is WAAAAAAYYYYYYY too big for iPhone use.
So I'd give it a 5 stars, if it fits your phone, and a 3 stars rating as an iPhone bag.
View More Details on SealLine e-Series Electronic Case


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JSB music is influenced by people with soul and groove, old and new. Al Green, Mofro, Otis, Soulive, G Love, James Brown, Subdudes, Jack Johnson, Gov't Mule, Big Head Todd, for example and the list goes on. "We are true fans of Soulful music!!!" states Sutton. "We are all about getting the crowd fired up and involved in the show. The music performed in our shows is all designed to get folks dancing, singing, and having a great time."
To quote a review in The Nashville Underground Music Scene, "Three guys who moved to Nashville to be apart of a thriving music scene that they are rarely part of due to their excessive road schedule. Their performances are fun, exciting, and full of great playing."
Over the past 11 years JSB has been performing for any and all crowds throughout the Southeast and Midwest turning their shows into crowd sing-alongs with both their original and cover music. From college club to private party this band loves to perform. As the band has grown, JSB has been through several line up changes. This JSB is truly at home on stage and on the road. A band of professional minded musicians they share a common goal of entertaining everyone who is kind enough to pay them a listen.
Their songs, written with expression and realism, are done so in a way that the purpose of the lyric is not lost in the live jam. Always fun!!! Always a party!!! Always a jam!!! The music comes first right along side the fans of their music. A truly appreciative band, with all the love and support they are getting from their fans, JSB is in this for the long haul. Rock!!! Soul!!! Blues!!! The John Sutton Band is jamming rock music for the lovers of rock music. At a JSB show you feel like you are jamming at the beach, dancing in the sand.


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5 Best Teams In NBA Heading Into 2014-2015 Season
The NBA season is just two weeks away. Let's break down who the five best teams in the NBA are.
5 Worst Teams In NBA Heading Into 2014-2015 Season
With the NBA season right around the corner, let's break down which five teams are the worst.
Top 10 NBA Rookies You Should Follow On Instagram
Here are the top ten NBA Rookies you should follow on Instagram.
5 Months When You Forget Sports Aren't The Only Thing Happening
Is there a better time of the year for sports than now? Well, depends on what you like. Here are five of the best sports months.
The 5 Biggest Athletic Endorsement Deals In The Last 10 Years
Athletes not only sign big contracts on the field but off the field as well. Most athletes make more money off of their endorsement deals than their actual contract salary.
Keidel: LeBron Is Cavalier In Cleveland
You've seen the sardonic messages splashed all over social media. Everyone is calling LeBron James the best GM in the NBA, based largely on his ability to wrench Kevin Love from Minnesota, which instantly imbues the Cavaliers with three All-Stars.
7 Notable NBA Players Who Have Played Overseas
In light of Metta World Peace aka Ron Artest aka The Panda's Friend announcing that he is playing in China next season, let's take a look at other notable players who have taken their talents overseas.
5 Favorites To Win 2015 NBA Title
With Kevin Love headed to Cleveland, let's take a look at the 5 favorites to win the 2015 NBA title.
Top 5 NBA Starting Lineups After The Kevin Love Trade
Kevin Love has finally been traded. With the deal in place, let's take a look at the top five starting lineups in the NBA…
20 Hysterical #NBASweets Memes Found On Twitter
20 of the best #NBASweets Memes Found On Twitter


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The right kind of tears filled tents and street corners as visitors spilled into Jonesborough last week for the 42nd Annual National Storytelling Festival.Comments
"We have received a lot of positive feedback from vendors and festivalgoers" - Unicoi County Chamber of Commerce Executive Director Amanda Delp.Comments
The 10-day campaign will feature special editions of public radio favorites like "Fresh Air," "Car Talk," "This American Life," "A Prairie Home Companion" and "Wait Wait Don't Tell Me!"Comments
Franco became fascinated with the art of magic after seeing it on television at age four.Comments
Series creators and executive producers David Lynch and Mark Frost will be back to write and produce the limited series.Comments
From "Frozen" to "The Little Mermaid," audiences will be enchanted by songs from classic Disney films at Milligan College's Homecoming concert.Comments
New York Times bestselling author, Gordon Korman visited Ridgeview School for a day and a half long celebration of reading and literacy on September 23, 2014.Comments
The Oct. 16 concert will begin at 7:30 p.m. at the Willow Tree Coffeehouse and Music Room, 216 E. Main St. Admission is free.Comments
Megan Jean and the KFB and Amythyst Kiah & Her Chest of Glass will perform at a masquerade party tonight at the Willow Tree Coffeehouse and Music Room in Johnson City. Tickets will be $10 at the door and the music starts at 9. Costumes are encouraged.Comments
The Historic Jonesborough Dance Society will host the next Contra Dance of the season on Saturday at the Pavilion at Serenity Knoll, 542 Bacon Branch Road, Jonesborough.Comments


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We created Pandora to put the Music Genome Project directly in your hands
It's a new kind of radio –
stations that play only music you like
True Love Way
Features of This Trackelectric rock instrumentation
a subtle use of vocal harmony
mild rhythmic syncopation
major key tonality
romantic lyrics
These are just a few of the hundreds of attributes cataloged for this track by the Music Genome Project.show more
Similar Tracks
Need to see you guys play live ! Come to Southern California !! Soon !!
Rarely a band with this sorta genre comes along and puts a touch and a spin to rock and it all becomes addictive and/or enjoyable
Love KoL


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About Port Douglas Australia
Port Douglas is the nearest mainland town to the Great Barrier Reef in Tropical North Queensland, Australia. The world heritage rainforests of the Daintree and Cape Tribulation are also at your doorstep, making this an extremely popular holiday destination for both Australian and overseas visitors. The Mossman Gorge, the Atherton Tablelands and outback Queensland are also close by.
Port Douglas boasts a superb tropical climate all year and combined with it's charming, friendly, village atmosphere, is the perfect place to "get away from it all"........somewhere to put your feet up and unwind. Whether you're a couple or a family with children, you'll find plenty to see and do during your holiday and will more than likely return home wishing you had booked to stay longer.
Port Douglas is also renowned for its fine restaurants, world-class shopping facilities, large choice of tours to the rainforest and reef, and the wide sandy stretches of Four Mile Beach. This is somewhere to put your feet up and unwind. Walk on Four Mile Beach in the morning, cruise the Great Barrier Reef or visit the rainforest on a tour during the day, then dine in one of the many famous restaurants in the evening. Scroll down for more details about accommdation, tours, restaurants, shopping, weddings.
Location of Port Douglas
site by jomarc
Camera Image Supplied by Zinc Restaurant & Lounge Bar - www.zincportdouglas.com
Port Douglas Australia - Port Douglas Web Camera & Tourist Information 2014


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28 year old man | Palos Verdes Peninsula, CA, USA | Seeking women 21-25 within 50 miles of Redondo Beach, CA
A word thats just stuck with me for awhile is the word PERSPECTIVE. I like putting things in perspective because it allows us to grow and understand one another. It makes me want to explore and meet all different kinds of people. I'm very animated but at the same time very mellow. I love anything creative and artistic and don't care much to conform to the masses, because I'm very passionate about street art and underground hip hop. I am Japanese and am very proud of our culture, yet love exploring and interacting with other cultures. I'm a hopeless romantic who has not given up yet while so many others have. I'm looking for a girl who is open minded, soulful, and is adventurous. A girl who is artistic in any way is a definite plus, whether she sings, plays instruments, or does poetry. My family used to own a Japanese restaurant, so I'm definitely a foodie and I do not eat to live, I live to eat! I definitely want to be with someone who enjoys good food, whether it's a hole in the wall or fine dining. I'm also looking for someone who is active and spontaneous because I have a long bucket list of things to explore and I'd love to share it with someone who's truly special. I tend to only watch comedies because I always have this yearning for something new to make me laugh, so I definitely want someone with a good sense of humor. Family is a very important part of my life, and I'd like a girl who's very family oriented. I've had a long line of girlfriends who were overly dramatic which has led me to avoid all the drama at all costs. I don't see myself who's not well-mannered because that is a big pet peeve of mine. Personality really comes before looks, but I guess I'm attracted to girls who have a natural beauty, girls who don't have to rely on all the make-up and fahsad. Fakeness is a disease so please don't spread it around.
Cooking , Dining out , Hobbies and crafts , Movies/Videos , Museums and art , Music and concerts , Exploring new areas , Nightclubs/Dancing
Billiards / Pool, Cycling, Other types of exercise, Swimming, Tennis / Racquet sports, Walking / Hiking, Weights / Machines, Yoga
I exercise 5 or more times per week.
I have Dogs, Fish, Other
I like Birds, Cats, Exotic Pets, Horses
I am obsessed with dogs! They are truly man's best friend and have nothing to give but love~
Some other viewpoint
I love places that have a lot of greenery and aesthetics, the ocean or any body of water, underground hip hop shows, spoken word, and anywhere that has good ambiance and vibes.
I love ALL genres, and yes even country. My heart is in Underground Hip Hop, my soul is in oldies and my vibes are in reggae. I enjoy dubstep and electronica and i like punk rock too! My TV shows would have to be South Park, The Office
I love anything artistic and creative. The sort of art I'm into isn't really conventional, but I'm really into graffiti and street art as well as underground hip hop. I can't forget about spoken word or anything that involves the ocean.
I started getting into this book about sacrogeometry, real interesting stuff! All is number!
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What he is looking for
|6'0" (182cms)
|5'3" (160cms) to 5'7" (170cms)
|Body type:
|About average
|Slender, Curvy, Athletic and toned
|Black, Brown, Hazel
|Black, Light brown, Dark brown, Blonde
|Occasionally, Yes, but trying to quit
|Social Drinker
|Social Drinker
|Education / Teacher / Professor
|Administrative / Secretarial, Artistic / Creative / Performance, Medical / Dental / Veterinary / Fitness, Self-Employed / Entrepreneur, Student, Education / Teacher / Professor, Other profession, Nonprofit / Volunteer / Activist, Fashion / Model / Beauty
I work with children from ages 3 to high school teaching mathematics and reading.
|$35,001 to $50,000
|$25,001 to $35,000
|Never Married
|Never Married
|Have kids:
|Wants kids:
|Asian, Latino / Hispanic, Pacific Islander, White / Caucasian
My father was a minister for the Church of Perfect Liberty. (Japanese religion)
|English, Japanese, Spanish
|Bachelors degree
|Some college
I went to UC Irving my undergrad but currently attending CSULB for my teaching credentials and hope to apply elsewhere to earn my masters degree...I eventually want to be self-employed and run a Kumon Center because I love children and mathematics!


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2009 NFL Draft
Post-Workout Rankings
The Top Guard Prospects
Pete Fiutak
2009 NFL Combine Numbers
| Running Backs & Fullbacks
| Receivers
Ends | Centers & Guards
Off. Tackles
Def. Ends |
Def. Tackles
Inside LBs
Outside LBs
new Cornerbacks |
new 2009 NFL Draft Post-Workout
Quarterbacks |
Running Backs |
Fullbacks |
Receivers |
Tight Ends
Centers |
Guards |
Off. Tackles |
Def. Ends |
Def. Tackles
Inside LBs |
Outside LBs |
Cornerbacks |
Full Player Breakdowns & Analysis To Come In March-
2008 CFN Draft Central
2008 NFL Draft Breakdown and Analysis
1st Round
2nd Round
3rd Round
4th Round
5th Round
6th Rd
7th Rd
1. George "Duke" Robinson, Oklahoma 6-5, 329
Pre-Combine Position Rank: 1Post-Combine Skinny: Duke
was every bit as big as expected, but he didn't lift. He ran an
eye-popping 5.27 for a player of his size.
Positives: Great in pass
protection … Quick. Can get on the move and make blocks down the field
if needed … Mauling when he gets his hands on a defender.
Negatives: Limited to guard.
He's not a tackle … Has gotten by on overpowering college defenders. Has
to prove he can handle linemen with his strength.2. Andrew
Levitre, Oregon State 6-3, 305
Pre-Combine Position Rank: 2Post-Combine Skinny: One
of the hot guard prospects coming into the Combine, he showed surprising
athleticism and ran well with a 5.24. However, the 23 reps were fine,
but nothing to get excited about
Positives: Smart … Very strong with good size … Not bad at getting
Doesn't have an elite NFL guard's body … Hard worker who isn't
necessarily gifted … Not an athlete.3. Kraig Urbik, Wisconsin
6-6, 335
Pre-Combine Position Rank: 3Post-Combine Skinny: He
looked the part. If Wisconsin linemen are considered among the safest
around to draft, he didn't do anything to slow down those beliefs. The
29 reps were enough, and he looked smooth in the 40.Positives: Could
play either tackle or guard … Excellent athlete for his size … Isn't bad
on the move.Negatives:
While physical, he's not as dominant as he should be … Not consistent
for his talent. 4.
Tyronne Green, Auburn 6-2, 309
Pre-Combine Position Rank: 4Post-Combine Skinny: While
he moved well, he quietly slipped through the Combine without showing
anything major, for good and bad.
Positives: Athletic … Tough. A former defensive tackle … Produced
against top-shelf SEC tackles.
Negatives: Still needs to develop a bit in his technique … Not
polished … Not a powerful run blocker.5. Herman Johnson,
Pre-Combine Position Rank: 5Post-Combine Skinny:
Johnson lost a ton of weight, and while he wasn't bad, he wasn't as
fluid as expected. He only lifted the 225 21 times, but that's partly
because of the longer arms.Positives: Huge.
HUGE. … Mauling when he locks on a defender … Long arms. Great in pass
protection.Negatives: Can
be beaten by quicker linemen … Not a fit for finesse offenses … Not
great on the move.6.
Louis Vasquez, Texas Tech 6-5, 333
Pre-Combine Position Rank: 8Post-Combine Skinny: He
wasn't just strong, he was phenomenally strong. He led everyone on the
day with a whopping 39 lifts on the bench. He used that power to blast
through the weight room.
Positives: Extremely quick for
his size … Great in pass protection … Great athlete who could play
tackle.Negatives: Not as
much of a mauler as he should be … Might not be suited for a power
offense despite his size and strength. 7.
Greg Isdaner, West Virginia 6-3, 325
Pre-Combine Position Rank: 7Post-Combine Skinny:
Considered the type of blocker best on the move. he ran a mediocre 5.44.
The 24 lifts were fine, but he didn't impress enough to show he was
something special.
Positives: Nice on the move …
Experienced, productive veteran … Good in pass protection.
Negatives: Not elite-level
strong … Shoulder concerns … Built like a tackle but is a guard.
Cedric Dockery, Texas 6-4, 320
Pre-Combine Position Rank: 6Post-Combine Skinny:
Wasn't At The Workout
Positives: Looks like the
prototype NFL guard …. Good in a phone booth … Very strong.
Negatives: Doesn't play up to
his potential … Mediocre pass protector … Not a great athlete.
9. Anthony Parker, Tennessee 6-2,
297Pre-Combine Position Rank: 9Post-Combine Skinny:
He didn't run the 40, but he lifted and came up with
a nice 24 lifts. Long wingspan. Positives:
Versatile. Could move to center … Great base with good balance …
Excellent all-around blocker.
Negatives: Lacks elite quickness … Doesn't dominate even when he
gets a hold on a defender … Could stand to be more of a mauler.
10. Trevor Canfield, Cincinnati 6-4, 307
Pre-Combine Position Rank: 10Post-Combine Skinny: The
former Bearcat had a strong workout. He lifted 20 times and was just
athletic enough to dominate at times.
Positives: Can play either
guard spot … Great athlete … Great for a finesse offense.
Negatives: Not a mauler … Can
be shoved around a bit … Has to keep weight in check.11.
Andy Kemp, Wisconsin 6-5, 313
Pre-Combine Position Rank: 11Post-Combine Skinny:
While not a difference maker, he didn't stand out in the workouts. He
was good, but nothing special.
Positives: Big body who's tough to get around … Tough. Played
through a broken hand.
Negatives: Not strong enough for his size … Not a good athlete. A
statue.12. Ramon Foster, Tennessee 6-5, 328
Pre-Combine Position Rank: 12Post-Combine Skinny: The
20 reps were a big problem for a player looking to shine on the inside.
He's big with long arms, and he didn't move all that well.
Positives: Big body … Good
worker. Good motor … Could be moved to tackle if needed.
Negatives: Not an elite pass
blocker … Has issues against speed rushers and quick linemen.
13. Travis Bright, BYU 6-4, 316
Pre-Combine Position Rank: 13Post-Combine Skinny: The
34 reps on the bench were among the best of the guards, and he showed
some explosiveness in the drills.
Positives: Insanely strong. Record-setting strong … Plays with a
mean streak … Very tough. Punishing.
Negatives: Old. He's already
26 … Not a good athlete … Durability a problem.14. Ray Feinga,
Pre-Combine Position Rank: 14Post-Combine Skinny:
While he wasn't all that quick or fluid, he proved he really is big, and
he did a good job on the bench with 30 lifts.Positives:
Dominant run blocker … Improved in pass protection … Strong with a
great, big body.Negatives:
Never lived up to the prep hype … Good, but not special. 15.
Mitch Petrus, Arkansas 6-3, 310
Pre-Combine Position Rank: 15Post-Combine Skinny:
Wasn't At The Combine
Positives: Athletic. He's a
beefed up tight end … Can play tackle … Hard worker.
Negatives: Might not be enough
of a mauler to play guard … Not a strong blocker and needs to be in a
zone blocking offense.16. Steve Rehring, Ohio State 6-7, 345
Pre-Combine Position Rank: 16Post-Combine Skinny: Wasn't At
The Combine
Positives: Solid run blocker …
Tackle-sized. Able to pound away when needed.
Negatives: Too tall to be a
regular guard … Wasn't consistent for the Buckeyes. Didn't have a great
senior year. 17.
Jim Tartt, Florida 6-3, 300
Pre-Combine Position Rank: 19Post-Combine Skinny: Wasn't At
The Combine
Positives: Tough. He fought
through injuries … Durable … Can dominate at times when 100%.
Negatives: Health … Not a
mauler. Better in pass protection.
18. Matt Slauson, Nebraska 6-5, 316
Pre-Combine Position Rank: 18Post-Combine Skinny:
One of the more athletic guard prospects. While big, he moved extremely
well and jumped well.
Positives: Versatile … Can
work at tackle if needed … Good run blocker who's better in power
offenses.Negatives: Hasn't
been enough of a power blocker … Needs to do more to shove around
Paul Fanaika, Arizona State 6-6, 327
Pre-Combine Position Rank: 17Post-Combine Skinny: He
slimmed down and was much lighter than expected, and it showed in the
bench press with a horrendous 15 reps. He didn't run.
Positives: Huge body …
Excellent upside with some development.
Negatives: Mediocre athlete.
Not great in pass protection … Needs work. Can't get on the move.20. Ryan Durand, Syracuse 6-4,
Pre-Combine Position Rank: 20Post-Combine Skinny:
Wasn't At The CombinePositives: Tough
blocker … Great worker who'll bust his tail to get better.
Negatives: Marginal athlete …
Doesn't do any one thing all that well.


text:
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Details on 'Ellen' segment with 'Milwaukeenos' airing Wednesday
TV talk show host Ellen DeGeneres gave cash gift cards to fans who wore ugly holiday sweaters to an event she staged in Milwaukee Tuesday.
She used Twitter to tease fans here with details of the Toys for Tots event throughout the day. »Read Full Blog Post(1)
Comedian Gaffigan savors New Year's in Milwaukee
Pasty comedian Jim Gaffigan has become such a New Year's tradition in Milwaukee that Point Brewery in Stevens Point bottles a special beer in his honor for the shows - aptly titled "Jim Gaffigan Pale Ale."
This will be the sixth year that Gaffigan, 46, performs at the Pabst Theater around New Year's. Beside the fact that tickets continue to sell well (he's playing three shows from Saturday through Monday this year) there's another important reason why he keeps coming back. »Read Full Article
Free and bargain ebooks for Kindle, Nook, ereader users
If someone gave you a Kindle, a Nook or another ereader for the holidays, or you're curious about reading ebooks on your new smartphone or tablet, here are a few sites to bookmark or follow via RSS to help you fill up your devices.
On Tap Today: Merry Christmas at the movies
If you're like my family, you get up on Christmas morning excited to open the gifts under the tree, to dig into a ham dinner, to spend quality time with loved ones...and then you need to get the heck out of the house. That's why I've spent many a Christmas night at the movies.
And of course, I'm not the only one. Christmas Day is one of the year's biggest holidays for new movie releases, and this year is no exception. »Read Full Blog Post


text:
Originally written on SI.com | Last updated 2/20/12
Washington Wizards Gilbert Arenas falls hard after committing a foul on the Miami Heat during the fourth quarter at the Verizon Center in Washington on November 4, 2009. The Heat defeated the Wizards 93-89. UPI/Alexis C. Glenn Photo via Newscom
Getty Images
Gilbert Arenas spoke with SI.com recently about everything, from that ill-fated gun incident in 2009, to his recent workout with the Lakers and his hope for the future.
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The Pajanimals Adventure
Go on an adventure with The Pajanimals! Visit Jerry the Bear's house in the Friendly Forest!
Go on an adventure with The Pajanimals! Visit Jerry the Bear's house in the Friendly Forest!
Create your own sandy stories with Nina from The Good Night Show.
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Learn Sign Language with Nina from The Good Night Show. Watch videos of Nina to learn then play in 'Play' mode to quiz yourself!
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|Publications at this Location
|ARS scientists publish results of their research projects in many formats. Listed below are the publications from research projects conducted at this location. Clicking on a publication title will take you to more information on the publication. Clicking on the reprint icon will take you to the publication reprint.
2015 Publications
(listed by order of acceptance date)
|Displaying 1 to 1 of 1 Records
Untapped genetic variability in Herefords: implications for climate change
(Abstract Only)


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Doesn't add anything new, doesn't inform you as much as previous versions
We all know that if you have a computer and it's hooked up to the Internet then you need as much protection as possible to stop it being infected by all and sundry.
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You won't find anything particularly new or ground breaking on offer, but you will find that everything has been tightened up. Take the anti-spyware, for instance, which has been greatly improved. Anti-phishing has also been strengthened but the thing we like the most is that the program no longer throws up as many alerts and pop-up boxes, simply choosing to deal with any potential threats in the background.
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If you want to delve deeper into our your security settings work, you can always access the Security Inspector, which scans your system for potential problems, such as weak passwords, vulnerable browser/IM settings and the like, which helps you tighten your defenses.
One of the big problems we've always had with Norton is the amount of system resources, especially memory, which the software has needed to run smoothly. Thankfully, this is no longer the case, as 2007 feels positively streamlined in comparison. Even on a low-powered PC, you'll hardly notice it's there.


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Headlines Blare Americans Lose 40% of Their Wealth Yet Income Decline is the Real Horror Story, by Robert OakQuicklink submitted by Ethan Hollow Permalink
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|The Federal Reserve released a report, the 2010 Survey of Consumer Finances. This is a report on household wealth from 2007-2010, removing effects of inflation. No surprise, median net worth declined by 38.8% from 2007 to 2010 and is down to 1992 levels. Why this should be no surprise is due to the housing bubble and declining home values. A home is the largest asset many people have.
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Sapporo - Sonny Bill Williams didn't get off to the start he hoped for in his debut in Japanese rugby on Sunday.
The star centre played the full 80 minutes but his Panasonic Wild Knights were defeated 25-22 by NTT Communications Shining Arcs in Japan's professional rugby league.
The Wild Knights were runners-up last season and were heavy favourites heading into Sunday's match with the addition of Williams.
Williams quit New Zealand's All Blacks to pursue more lucrative opportunities in Japan and Australia. He is reportedly earning $1.2m for his 12-game stint in Japan.
After Japan, Williams will play in Australia's National Rugby League.
A try by hooker Tetsuya Shitara gave Panasonic a 22-3 lead four minutes into the second half but the Shining Arcs fought back with tries from Yoshio Kimishima, Samoa international Alesana Tuilagi and Hiraku Tomoigawa.
Kimishima also knocked over two conversions and two penalty goals to finish the day with 15 points as NTT picked up their first win of the season.
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text: youth community coalition Drug Take Back Program visits Columbia By Jonathan Fenton • Oct 24, 2013 The Drug Take Back Program is collecting prescription drugs today in Columbia. Ryan Worley, Coordinator of the Youth Community Coalition, is in charge of the program. He said unused prescription drugs can sometimes be accessible to and abused by family members if they are not disposed of properly. "These are not safe to abuse. They are drugs they are powerful, and they should only be taken the way a doctor prescribes them," Worley said. Youth advocacy group to host drug take-back event By Katy Mersmann • Apr 26, 2013 The Javorac / Flickr Listen Listening... / 2:11 The Columbia-based Youth Community Coalition is hosting its annual prescription drug take back this Friday and Saturday. The take back is part of a national movement to collect and dispose of expired and unused prescription drugs, in collaboration with the U.S. Drug Enforcement Administration.


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Limited-time feature
35 year old man | Santa Monica, CA, USA | Seeking women 25-35 within 15 miles
Never judge a book by its cover.
I have a very easy going personality and I love to make people laugh even at the expense of me looking like a complete fool. I like to work with absolutes, which helps me formulate a plan and achieve personal goals. Once I establish a goal I will work tirelessly to achieve it. A large part of what I do for a living is mentoring and developing others to do better and perform optimally. I'm very passionate about my work and I'm thankful every day that I was able to steer myself into this field and role where I wouldn't trade it for anything in the world. Lately I've been trying to check doing a triathlon off the list. I've been training for about 6 months now and am doing a half iron-man coming up in April and another two weeks after that. I'm generally very active, and outdoorsy. I love to fish, hike, bike, camp, climb, hanging at the beach. After long days of working out or being outside I decompress with a movie and some beverage. I'm looking for someone genuine, strong willed and passionate about their life. A girl that accepts no constraints regardless of circumstances. A partner that is capable of surviving through the thick of it in life, no bullshit or stuck on petty things, and most importantly doesn't take herself too seriously. You love to have a good time, music, art and travel. I know you're out there...
Camping , Coffee and conversation , Fishing/Hunting , Movies/Videos , Museums and art , Music and concerts , Exploring new areas , Nightclubs/Dancing , Performing arts , Playing cards , Playing sports , Travel/Sightseeing , Video games , Watching sports , Wine tasting
Cycling, Dancing, Hockey, Martial arts, Other types of exercise, Running, Skiing, Swimming, VolleyBall, Walking / Hiking, Weights / Machines
I exercise 5 or more times per week.
I like Birds, Dogs, Exotic Pets, Fish, Horses
Middle of the Road
The beach, hot tub, lounging, movies, fun places to eat and night clubs.
I love 80's movies: Mannequin, Top Gun, Rocky, Willow, Princess Bride, and Big Trouble in Little China...More recent ones: Silver Linings, Skyfall, The Bourne Identity, and Tron, Intouchables, Life of Pi.
I'm generally very active, and outdoorsy. I love to fish, hike, bike, camp, climb, hanging at the beach.
Ender's Game or epic adventure books.
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What he is looking for
|6'4" (193cms)
|5'3" (160cms) to 6'0" (182cms)
|Body type:
|Athletic and toned
|Slender, About average, Athletic and toned
|Black, Blue, Brown, Grey, Green, Hazel
|I'll tell you later
|No preference
|No Way
|No Way
|Social Drinker
|Social Drinker
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|Associates degree
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text:
|Dog Days are Over
|20m. Awesome diagonal line starting from the depths of the zawn and heading leftwards along a faint crack.
Start as for "Support your local sheriff" / "Deputy dawg", follow the diagonal undercut crack into "Department of Correction", crux moves to gain and leave a two finger pocket and a pumpy finish lead to a lower off at a small triangular hand ledge on the arete.
*Can also be started up "Department of Correction" at the same grade
M.Richards and A.Sharp 05/Jun/2011
Submit a new photo of this climb.