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College students with no loan debt are more likely to lead a richer social life that involves partying, studying less, and forming relationships that will last long after graduation, a pair of University of Indiana sociologists says.
People may wait until the last minute to pay a bill, but when owed a refund, they want it as quickly as possible. Understanding that the manual refund process at American Public University System (W.Va.) was problematic, the finance staff set out to improve it and ended up reducing average processing time for financial aid refunds by 90 percent.
Any efficient solution to the explosion in college tuition and fees must either damp demand or increase supply.
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When politicians make a stink about student loan rates, they're smelling a rotten fish, but not the most obvious one.
Statistics analyzed by the federal Consumer Financial Protection Bureau show that more than six million borrowers are in default on their federal student loans, representing $89 billion of the more than $1 trillion in outstanding debt.
The House on Wednesday approved a bipartisan bill that ensures lower interest rates on loans for students heading to college this fall. Members of the House voted 392 to 31 to lower rates for undergraduates taking out government loans this school year to 3.86%.
College students have a better chance of getting financial aid if they come from affluent backgrounds than if they are lower on the income scale, some new studies show.
A comparison of low-income enrollment shows wide disparities among the most competitive private colleges. A student at Vassar, for example, is three times as likely to receive a need-based Pell Grant as one at Washington University in St. Louis.
Nearly one in five community college students get so worried about finances, they think about dropping out, according to the "Inceptia National Financial Aptitude Analysis" report. Teaching them to better manage money may help them graduate.
The number of students applying for federal financial assistance jumped 62 percent over five years, while the total amount of federal financial aid awarded to students under Title IV of the Higher Education Act also increased dramatically. Information about these vital programs can be found in NASFAA's recently updated National Student Aid Profile.


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The US Army wants to buy a new family of high speed rotorcraft by 2030. Four companies have been awarded study contracts by the Army Aviation Technology Directorate (AATD) — AVX Corp, Bell Boeing, Boeing and Sikorsky. Three of the four concepts were on display in the exhibit hall of the Association of the US […]
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AUSA2011: Boeing displays ideas for next-gen rotorcraft
The US military's helicopter replacement master plan
The US military plans to reduce about 25 different helicopter types today into four models of a next-generation of vertical lift aircraft. We've known this for a few years already, but the full scope and vision for the Future Vertical Lift (FVL) studies guiding the Joint Multi-Role (JMR) has eluded this blogger until now. The […]
Russia talks stealthy, 370mph combat helicopter
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Definitions for callie
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A 19th century diminutive of Caroline, also used as a formal female given name.
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All jawed-vertebrates have four T cell receptor (TCR) chains: alpha (TRA), beta (TRB), gamma (TRG) and delta (TRD). Marsupials appear unique by having an additional TCR: mu (TRM). The evolutionary origin of TRM and its relationship to other TCR remain obscure, and is confounded by previous results that support TRM being a hybrid between a TCR and immunoglobulin locus. The availability of the first marsupial genome sequence allows investigation of these evolutionary relationships.
The organization of the conventional TCR loci, encoding the TRA, TRB, TRG and TRD chains, in the opossum Monodelphis domestica are highly conserved with and of similar complexity to that of eutherians (placental mammals). There is a high degree of conserved synteny in the genomic regions encoding the conventional TCR across mammals and birds. In contrast the chromosomal region containing TRM is not well conserved across mammals. None of the conventional TCR loci contain variable region gene segments with homology to those found in TRM; rather TRM variable genes are most similar to that of immunoglobulin heavy chain genes.
Complete genomic analyses of the opossum TCR loci continue to support an origin of TRM as a hybrid between a TCR and immunoglobulin locus. None of the conventional TCR loci contain evidence that such a recombination event occurred, rather they demonstrate a high degree of stability across distantly related mammals. TRM, therefore, appears to be derived from receptor genes no longer extant in placental mammals. These analyses provide the first genomic scale structural detail of marsupial TCR genes, a lineage of mammals used as models of early development and human disease.
The hallmarks of the vertebrate adaptive immune system are antigen specific receptors, the T cell receptors (TCR) and immunoglobulins (Ig) encoded by genes that undergo somatic DNA recombination to generate diverse binding specificities. The TCR are expressed by thymus-derived lymphocytes (T cells) that play a major role in regulation and effector functions of immune responses. Each T cell expresses a unique TCR that binds a specific antigen resulting in the activation of an immune response [1,2]. TCR are heterodimers comprised of either alpha (TRA) and beta (TRB) or gamma (TRG) and delta (TRD) combinations, respectively. These two combinations define the two major lineages of T cells: αβT cells and γδT cells [3,2]. αβT cells typically recognize peptide antigens presented on major histocompatibility complex (MHC) encoded molecules. In contrast γδT cells have been shown to be either MHC restricted or in some cases, similar to Ig, able to bind free antigen [4]. Ig are expressed by antibody forming cells (B cells), which produce both a membrane bound form of Ig that comprises the B cell receptor (BCR) and a soluble form that is free antibody. Like TCR, Ig are made up of two different chain types, a heavy (IgH) and light (IgL) chain. TCR and Ig chains both contain variable domains that bind the antigen and membrane-proximal constant (C) domains. It is the variable domains that are encoded by gene segments that undergo somatic recombination to generate diversity in binding specificity. The gene segments encoding the variable domains of TRA, TRG and IgL chains are the variable (V) and joining (J) gene segments, while the variable domains of TRB, TRD and IgH chains are encoded by exons assembled from V, diversity (D) and J gene segments [5]. Recombination of these gene segments takes place in the thymus for developing T cells and adult bone-marrow for developing B cells [5].
Of the immunoglobulin superfamily (IgSF) members, the TCR and Ig are each other's nearest relatives, however there are dissimilarities to their genetic structure and evolutionary history [6,7]. For example all jawed-vertebrates appear to contain the same four homologous TCR isotypes: TRA, TRB, TRG, and TRD [8]. In contrast there is variability in the number and class of Ig isotypes in different vertebrate lineages [9,10]. In addition the organization of TCR loci appears to be more conserved than Ig. For example, in cartilaginous fish most Ig loci are organized as multiple, unlinked clusters of [V-(D)-J-C], limiting the combinatorial usage of their gene segments [11]. Whereas, in bony fish and tetrapods the majority of Ig loci are organized in the translocon style of Vn-(D)n-Jn-Cn [11]. TCR loci tend to be organized in the translocon style in all lineages. These differences between TCR and Ig genes are likely the result of dissimilar selection forces on the two different antigen receptor systems and have made determining the evolutionary relationship of the TCR and Ig chains to each other unclear [12].
The relationship between Ig and TCR is further muddled by the recent discoveries in marsupials and sharks of TCR loci that appear to be hybrids between ancestral Ig and TCR loci [13,14]. In marsupials, a mammalian lineage that diverged from eutherians (placental mammals) 186 to 193 million years ago (MYA), a new fifth TCR chain, named TCR mu (TRM) has been identified [14,15]. Unlike the conventional TCR, TRM has a tandem cluster organization and its origins appear to have involved a recombination between and ancestral TCR locus, most likely a TRD and IgH. TRM appears analogous to an unusual shark TCR called NAR-TCR, which utilizes the C regions of TRD and upstream Ig-like V regions [13]. Both TRM and NAR-TCR are expressed in an atypical TCR isoform that contains double variable domains. Marsupial TRM and shark NAR-TCR, however, are not orthologous but rather the product of convergent evolution generating common features [14]. Nonetheless the presence of TCR with these features in both marsupials and cartilaginous fish, make it likely analogous TCR will be found in other vertebrate lineages and illustrate a level of plasticity in TCR evolution heretofore unrealized.
The availability of the first completely sequenced marsupial genome provides the opportunity to investigate the evolutionary origins of TRM and its relationship to the conventional TCR in mammals [16]. Towards this aim we have determined the complete genomic organization, content and evolution of the loci encoding both the conventional TCRs (TRA, TRB, TRG and TRD) and the recently discovered TRM locus in the opossum Monodelphis domestica. In addition, these analyses provide a level of detail for the TCR genes of a marsupial that has only been available for a limited number of eutherians such as human and mouse.
Results and Discussion
TRA/D locus
Previous physical mapping of the TCR loci in the opossum revealed that the TRA and TRD were co-localized on chromosome 1p [17]. Analysis of the opossum whole genome sequence confirmed that the TRD genes are clustered within the TRA locus resembling the organization of TRA/D observed in eutherians and birds (Figure 1) [18-20]. In opossum, the TRA/D locus spans approximately 1.3 Mb, making it intermediate in size compared to that of human at 1 Mb and mouse at 1.65 Mb [21,22]. To investigate the degree of genomic conservation in this region we identified the genes syntenic to opossum TRA/D locus and compared these to the available genomic data for mouse, human, cow and chicken using the current ENSEMBL databases for each species [23]. At the 5' end of the opossum TRA/D locus are the methyl-transferase like 3 (METTL3), zinc finger protein (SALL2) and several olfactory receptor (OR) loci that have conserved synteny in human, mouse, and cow, but not chicken (Figure 1 and Additional file 1). However these genes are not immediately flanking the cow TRA/D locus. Also conserved at the 5' end of the TRA/D locus in opossum, human, and mouse are TRA V genes (TRAV) interspersed with the OR loci. In opossum and mouse there is only one TRAV segment interspersed with the OR, whereas in human there are two at this location (Figure 1) [22]. These TRAV gene segments (opossum TRAV1, mouse TRAV1, and human TRAV1.1 and 1.2) appear orthologous in phylogenetic analyses forming their own distinct group (group B) in a tree of TRAV and TRDV sequences (Figure 2). The 3' end of the TRA/D locus appears to be the most conserved across species since many of the loci have conserved synteny in both mammals and birds. The opossum has two copies of the defender against cell death gene 1 (DAD1) also found at the 3' end of the human, mouse, cow and chicken TRA/D loci. In those eutherian mammals examined the position of the abhydrolase domain-containing protein 4 gene (ABHD4) is also conserved (Figure 1 and Additional file 1). The opossum TRDV6 gene segment further illustrates the conservation of the TRA/D locus across mammals. This gene segment is in an inverted orientation and located downstream of the TRD C (TRDC). A clear ortholog of TRDV6 is found in both human and mouse with the same location and reading orientation and these gene segments from all three species fall into the same phylogenetic clade (see below, Figure 2). These results all support the overall organization of the mammalian TRA/D loci and their flanking genomic regions being highly conserved over a span of at least 186 My and as long as 300 My in some cases [15,24].
Additional file 1. Opossum TCR syntenic genes and their corresponding location on chromosomes of several species. this table contains the opossum TCR syntenic genes and their corresponding location on chromosomes of several species.
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Figure 1. Map of the opossum TRA/TRD locus. TRAV (red), TRDV (yellow), TRDJ (light green), TRAJ (dark green) and TRDD (orange) gene segments are shown numbered by their location in order across the locus. TRV segments are designated with the subgroup number followed by a period and a designated number according to their location. TRA segments that were also found expressed with TRDC, and therefore TRA/DV, have diagonal yellow stripes. TRDC (light blue) and TRAC (dark blue) are also indicated. Transcriptional orientation is indicated by the direction of the arrow on each segment. Presumptive pseudogenes are indicated with a ψ. Syntenic genes discussed in the text are indicated in gray.
Figure 2. Phylogenetic tree of the TRAV, TRDV and TRA/DV gene segments from mammalian and avian species using the neighbor joining method. Opossum TRV sequences are indicated in bold. Mammalian gene segments that are known to be expressed as TRA/DV are indicated with an asterisk. TRV genes fall into eight groups (A-H, indicated with braces) which indicate the evolutionary relationship of these genes. Branch supports are indicated as the percentage of trees based on 1000 bootstrap replicates.
Overall the organization and complexity of gene segments within the opossum TRA/D locus is similar to that of human and mouse. There are 74 total V segments (TRAV plus TRDV) in opossum, a number intermediate to that of human and mouse (Table 1). In human and mouse the V gene segments are either TRAV or TRDV, in other words used in TRA or TRD chains respectively, or in some cases specific V segments have been found expressed in either TRA or TRD chains and have been designated TRA/DV. The category a particular V segment falls into is historically defined by a number of criteria. One criterion is nucleotide similarity to V segments defined already in other species; by this criterion there are 68 opossum TRAV and six TRDV segments when compared with human and mouse. These two groups, with a single exception, are spatially separated in the TRA/D locus with the TRAV segments at the 5' end and the TRDV at the 3' end of the locus. The exception is a single TRDV segment (TRDV1) interspersed with the TRAV segments (Figure 1). Of the 68 TRAV and 6 TRDV, 12 and 2 respectively appear to be pseudogenes due to absence of a complete open reading frame (ORF) (Figure 1, Table 1). This is a ratio of functional to non-functional gene segments comparable to human and mouse (Table 1).
Table 1. Number of TRV, TRD, TRJ and TRC genes found in human, mouse and opossum. The numbers of functional genes are indicated in parenthesis.
To determine which of the 74 V gene segments are also used as TRA/DV we performed RT-PCR on 23 day old thymus RNA using combinations of primer pairs specific for each of the V segment subgroups (see below) paired with either TRA or TRD C regions (TRAC and TRDC respectively) (not shown). This age was chosen as an early age where the thymus is fully mature [25]. Nineteen of the TRAV segments and all four of the functional TRDV segments were found expressed with both TRAC and TRDC resulting in a total of 23 apparent TRA/DV segments (Figure 1 and Table 1). The number of opossum TRA/DV may be an underestimate since it is possible some TRAV may be rarely expressed with TRDC or may appear at different times during development than was examined. Alternatively this number could be an overestimate as well, since it is possible that some combinations are negatively selected in the thymus and not used in the periphery. Either way this appears to be a comparatively high number of TRA/DV segments in the opossum relative to human and mouse (Table 1).
V gene segments evolve by gene duplication and deletion resulting in degrees of relatedness amongst segments [26]. These are defined as subgroups with the segments belonging to the same subgroup by having 80% or greater nucleotide identity. By this criterion the current 68 TRAV segments can be placed into 41 subgroups, where the nucleotide identity between subgroups ranges from 32.1 to 79.5%. The six TRDV gene segments were sufficiently different, with nucleotide identity ranging from 33.6 to 61.9%, that each belonged to its own distinct subgroup. Phylogenetic analyses using TRAV and TRDV segments were performed to elucidate their evolutionary relatedness. Opossum sequences were compared with sequences from human, mouse, rabbit, cow, sheep, and chicken using the same dataset as used by Su et al. [27] to define the major phylogenetic groups (Figure 2). Eight groups of V gene segments (designated A through H) with bootstrap values greater than 89% emerged from the inclusion of the marsupial sequences (Figure 2). All eight contain opossum sequences (Figure 2). Four of these, groups A through D, were defined previously and only included TRAV or TRAV/D [27]. However, the addition of opossum sequences revealed four new V groups (E through H) not previously recognized or requiring reevaluation. Group E contains TRAV, TRDV and TRA/DV sequences; groups F and G only TRDV sequences; and group H TRAV and TRA/DV sequences. The addition of the opossum sequences to this analysis substantiate the statement that all these subgroups were present in the common ancestor of amniotes and that some species have lost segments that belong to different subgroups [27].
These analyses also allow us to evaluate the evolution of mammalian V segments that can be utilized with either TRA or TRD chains over a larger time-span than has been available previously. TRA/DV sequences clearly belong to different groups rather than forming a monophyletic cluster (Figure 2) and, as observed for human and mouse, TRA/DV regions are dispersed throughout the opossum TRA/D locus, although most are located towards the 3' end of the locus (Figure 1). Considering that αβ and γδT cells recognize potentially very different antigens these results continue to support the high level of plasticity in V gene utilization at this locus.
The number and complexity of D and J gene segments in the opossum TRA/D locus is also comparable to that of human and mouse (Figure 1, Table 1). Encoding TRD chains are at least two D and six J gene segments (TRDD and TRDJ respectively), all of which are located upstream of a single TRDC. There are at least 53 TRAJ segments located between the TRDC and TRAC all of which appear to be functional by the criteria defined above for the V gene segments (Figure 1, Table 1).
The opossum TRAC and TRDC regions are encoded by three exons (Figure 3A, 3B) that, as reported previously, encode residues conserved in other species [28,29]. For both TRAC and TRDC, exon 1 encodes an IgSF domain, which contains two cysteine residues that form the intra-chain disulfide bond. Exon 2 encodes the connecting peptide (Cp) containing the cysteine residue involved in the inter-chain disulfide bond. Exon 3 encodes the transmembrane (Tm) and a short cytoplasmic (Ct) region (Figure 3A, 3B). In the TM region of both TRA and TRD there are two hydrophilic residues (lysine and arginine) that are conserved in other species and that are important for interaction with other dimers from the TCR complex [30]. There are five potential N-glycosylation sites in the opossum TRAC and two in the TRDC [28,29].
Figure 3. Exon and intron organization of the opossum TRC genes. Numbers above and below indicate the length of the exons and introns in base pairs, respectively. Indicated in white circles are the donor splice sites and in black the acceptor splice sites. A: TRAC; B: TRDC; C: TRBC1; D: TRBC2; E: TRBC3; F: TRBC4; G: TRGC.
TRB locus
TRB has been physically mapped to chromosome 8q in the opossum [17]. The opossum TRB locus spans 400 kb making it smaller in size than its human and mouse homologues that are about 650 kb each (Figure 4) [31,22]. Genes syntenic the opossum TRB locus are also conserved across human, mouse, cow and chicken. These include the trypsinogen genes (TRY) found at the 5' and 3' ends of the TRB locus and intermixed between the TRBV and TRBC gene segments (Figure 4) [22]. The mono-oxygenase DBH-like 2 (DBHL) is found at the 5' end of the locus in the opossum, similar to human, mouse, and cow, but not in chicken. Genes such as kell blood group glycoprotein (Kel) and ephrin type-b receptor 6 precursor (EPHB6) found at the 3' end of the opossum TRB locus have conserved synteny in mammals and chicken (Figure 4). As with TRA/D, the TRB locus organization is highly conserved between opossum and eutherians. This is further illustrated by a TRBV segment (TRBV28 in opossum) located at the 3' end of the locus that is in the reverse orientation relative to the other gene segments. A clear orthologue of this gene segment is present in human (TRBV30) and mouse (TRBV31) making this an ancient arrangement (Figure 4 and group F in Figure 5).
Figure 4. Map of the opossum TRB locus. TRBV (red), TRBD (orange), TRBJ (green) and TRBC (blue) are indicated. Transcriptional orientation, pseudogenes, and syntenic genes discussed in the text are indicated as in Figure 1.
Figure 5. Phylogenetic tree of the TRBV gene segments from mammalian and avian species using the neighbor joining method. Opossum TRBV sequences are indicated in bold. The percent bootstrap values based on 1000 replications are indicated. Major phylogenetic groups (A-F) are indicated with braces.
There are 36 opossum TRBV segments (Figure 4, Table 1) and these can be grouped in 28 subgroups based on nucleotide identity with the value between subgroups ranging from 38.5 to 68.5%. Compared with other TCR, the TRB locus in eutherians appears to contain a higher number of V pseudogenes, where 19% and 34% of TRBV are pseudogenes in human and mouse, respectively. This pattern appears to hold for the opossum since nine of the 36 TRBV segments (25%) appear to be pseudogenes (Table 1).
Phylogenetic analyses of the TRBV regions from different mammals and chicken reveal that the opossum TRBV regions are very diverse with six groups (A to F) identified (Figure 5). Human, mouse and opossum TRBV sequences are found in all six groups, supporting their presence before the divergence of marsupial and eutherian mammals (Figure 5) [27]. Three sequences chickenB1S1, mouseB2 and opossumB20 did not cluster with any other sequence, nor with each other, and therefore not included within any of the groups.
As in human and mouse, the opossum TRB D, J, and C genes are organized in tandem cassettes. The human and mouse TRB locus contains two of such D-J-C cassettes while the opossum has four (Figures 3C–3F and 4). In the opossum, each cassette contains a single TRBD, four or five TRBJ, and a single TRBC. All four cassettes appear to be functional and TRBJ gene segments from each have been found in TRB transcripts (data not shown).
The four opossum TRBC regions are very similar to each other at the nucleotide level and in intron – exon organization. Each TRBC region is encoded by four exons (Figure 3C–F). Exon 1 encodes the immunoglobulin domain which contains two conserved cysteines residues important for the intra chain disulfide bond formation. There are three potential N-glycosylation sites in exon 1. Exon 1 of TRBC1, TRBC2 and TRBC3 have 100% nucleotide identity and TRBC4 differs by only a single non-synonymous nucleotide substitution (K10E) that encodes a lysine (AAA) instead of the glutamate (GAA). Exon 2 encodes the Cp and it contains a conserved cysteine residue used for the inter chain disulfide bond (Figure 3C–F). The Cp from the four opossum TRB share more than 82.6% nucleotide identity. Exon 3 encodes the Tm region and contains a lysine residue involved in the interaction with the CD3 complex. The Tm regions are much conserved among the four opossum TRBC, sharing greater than 85.1% nucleotide identity. Exon 4 encodes the cytoplasmic region and it also includes 3' untranslated region.
Due to the high degree of sequence similarity among the four cassettes described above it is difficult to fully reconstruct the duplication events that led to the current arrangement in the opossum. However, cassettes 2 and 3 share two characteristics indicating they are derived from a relatively recent tandem duplication. First of all, both cassettes are nearly identical in nucleotide sequence over a 12.8 kb region that extends from 4.8 kb upstream of a non-functional copy of cyclin A2 including the gene segments TRBD, TRBJ to 100 bp downstream of TRBC (Additional file 2). Secondly both cassettes 2 and 3 have a cyclin A2 gene 5' of the D-J-C gene segments, which is not present in the other two cassettes (Figure 4). Cyclin A2 is also associated with the cow TRB locus but is not in human, mouse or chicken. This is consistent with the cyclin A2 gene being inserted near the TRB D-J-C cassettes prior to the divergence of marsupials and eutherians, with subsequent loss in some eutherian species such as human and mouse.
Additional file 2. Dot plot analyses of opossum TRB cassettes. The dot-matrix analysis corresponds to the comparison of 51 Kb region containing the four TRB D-J-C cassettes aligned to itself.
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TRG locus
TRG has been physically mapped to chromosome 6q in the opossum [17]. As in human and mouse the TRG locus is the smallest and least complex of the three conventional TCR loci. From the most 5' V to the 3' untranslated region (UTR) of the single C region, the opossum TRG locus spans only approximately 90 kb (Figure 6), smaller than that in human (150 kb) and mouse (205 kb) [32,33]. The opossum TRG locus has a translocon organization, which is different from that present in human and mouse (Figure 6). The human TRGV segments are upstream of two TRGJ-TRGC cassettes, while in mouse there are four cassettes that contain TRGV, TRGJ and TRGC gene segments [34,33]. Even though the organization of V, J and C gene segments appears different between human, mouse and opossum, the genes flanking this locus are conserved among these species (Figure 6). Amphiphysin (AMPH) and the related to steroidogenic acute regulatory protein D3-N-terminal like (STARD3NL) are found at the 5' and 3' end of the TRG locus respectively (Figure 6)[22]. Both AMPH and STARD3NL are also associated with the TRG locus in cow and chicken, although their locations appear changed when searching in their current assembled genomes [35]. In cows and sheep there are two TRG loci, TRG1 and TRG2 [36,37]. To determined if the opossum also has more than one TRG locus we examined its genome thoroughly by performing BLASTN searching of the entire MonDom5.0 assembly using the TRG cDNA and genomic sequences. Only a single TRG locus was identified and this corresponds to the locus we mapped previously to chromosome 6q (Figure 6) [17].
There are nine TRGV gene segments present in the opossum and these are divided into four subgroups based on nucleotide identity (Figure 7). All TRGV segments appear to be functional, and have been found expressed in the thymus (not shown). The number of TRGV segments is similar to that in mouse where there are seven V segments, all of which are functional. In human there are fourteen V segments, but only six are functional (Table 1).
Figure 7. Phylogenetic tree of the TRGV gene segments from mammalian and avian species using the neighbor joining method. Opossum TRGV sequences are indicated in bold. The percent bootstrap values based on 1000 replications are indicated. Major phylogenetic groups (A-J) are indicated with braces.
Previously, phylogenetic analyses of the mammalian and avian TRGV segments revealed the presence of eight ancient groups [38]. Addition of the opossum TRGV sequences to these analyses revealed two additional groups, group I and J. Group I contains human, mouse and opossum TRGV4. Support for this group is low (Figure 7), however it is likely that these three sequences from opossum, human and mouse are derived from the same ancestral gene since they also have similar RSS sequences (data not shown). The new group J contains only the members of the opossum TRGV1 subgroup (Figure 7), which is related to the previously defined group F that contains sequences from human, rabbit, sheep and cow [38]. This relationship is not well supported however, and two groups may not have arisen from a common ancestral gene segment (Figure 7). Opossum TRGV3 segments group in a previously defined group A. The single member of opossum TRGV2 subgroup does not clearly cluster with any existing group (Figure 7).
There is only a single opossum TRGC region (Figure 3G and 6) in contrast to four in mouse (three of which are functional) and two in human (both functional) (Table 1). The opossum TRGC is encoded by three exons. Exon 1 encodes the immunoglobulin domain and contains a single N-glycosylation site. An unusual characteristic described previously in marsupials was the absence of the second cysteine residue required for the formation of intrachain disulfide bond in the TRGC region [38]. Exon 2 encodes the Cp and exon 3 encodes the Tm, Ct and 3'UTR regions (Figure 3G).
TRM locus
The TRM locus has been described so far only in marsupials and is located on chromosome 3q in the opossum [14]. Homologs to TRM have yet to be found in any eutherian mammal examined so far (results not shown). Previous analyses of the TRM locus were consistent with TRM being a hybrid locus generated by recombination between ancestral Ig and TCR genes [14]. To examine this hypothesis further we analyzed the genes flanking the TRM locus to look for any evidence of this recombination. On the immediate centromeric, 5' side of TRM are three zinc finger protein genes of the C2H2 type (ZNF3) (Figure 8 and Additional file 1). Unfortunately these share similarity to human and mouse ZNF3 genes on several chromosomes making orthology difficult to establish (data not shown). On the telomeric, 3' side of TRM are genes encoding Speckle type POZ-like protein (PCIF1-like) and Myelin Oligodendrocyte Glycoprotein (MOG). In both cases PCIF1 and MOG have paralogous copies in the opossum genome and the paralogue syntenic to TRM is the least similar to the eutherian homologue. None of the genes flanking the opossum TRM locus have conserved synteny in human and mouse making it difficult to identify a region of the eutherian genome that is homologous to the region of the opossum genome containing TRM. In other words, and in contrast to the conventional TCR, the chromosomal region containing TRM is not well conserved in mammals.
Figure 8. Map of the opossum TRM locus. TRMV regions are colored in red, TRMD in orange, TRMJ in green, TRMVj in yellow and TRMC in blue. Clusters are numbered starting at the 5' end with V, D, J gene segments and C regions numbered according to the cluster to which they belong. All other designations are as in Figure 1.
Previously we reported that the TRM V gene segments appeared to be more similar to Ig V gene segments than that of TCR [14]. This conclusion was drawn from an analysis of a limited number of available marsupial TCR V gene segments at that time. Availability of the complete TCR genomic sequences described above allows us to further test this observation. TRM is organized in tandem clusters where complete clusters contain two classes of V segments: a single non-rearranged V gene segment (TRMV) and an unusual V gene segment which is already joined to D and J genes (TRMVj) in the germline DNA [14]. The opossum has six such complete clusters. The remaining two clusters are partial, lacking the TRMV and TRMD gene segments (Figure 8) [14].
To investigate the evolutionary history of the individual TRM clusters we compared the sequence and organization of the six complete clusters (clusters 1, 2, 3, 4, 5, and 7) and two partial clusters (clusters 6 and 8). These analyses revealed three classes of clusters based on gene content and nucleotide sequence identity. These three classes likely represent the most ancient duplications of TRM clusters. The lineages generated by these older duplications are represented by clusters 1, 2 and 3, respectively. Clusters 5 and 7 have similar gene content (three TRMD segments) and share greater nucleotide sequence identity to cluster 3 and represent more recent whole cluster duplications that would have followed the duplication of an additional D segment in this lineage [14]. Clusters 4, 6, and 8 contain two TRMD segments and share greatest nucleotide sequence identity to cluster 2, representing another round of more recent duplications. Cluster 1 is a third class unto itself based on not sharing significant sequence similarity to the others [14]. These results are consistent with the current complement of TRM clusters in opossum being the result of whole cluster duplications followed by divergence of each cluster. These duplication events have resulted in partial clusters cases and different numbers of clusters in different marsupial species; bandicoots for example appear to have only two TRM clusters [14,29].
Opossum TRMV and TRMVj each form distinct clades in a phylogenetic analysis and share only 38 to 43.5% nucleotide identity to each other and are, therefore, from distinct subgroups (Figure 9A) [14]. Now having the complete genomic sequence from the opossum, we wished to compare the TRMV genes with V genes from the conventional TCR and Ig loci. When TRMV and TRMVj are compared to all conventional opossum TCR V gene segments the similarity also remains low; nucleotide identity between TRM V genes and TRA, TRB, TRG, and TRD V genes ranges from 27 to 43%. In other words, the TRA/D, TRB, and TRG loci do not contain V segments from which either TRMV or TRMVj appear to have been derived. The greatest similarity for TRM V genes remains with the IgH V gene segments (VH) with nucleotide identity ranging from 34 to 51%. However, the clades of TRMV and TRMVj remain outside those containing VH genes from a variety of species (Figure 9A). In addition we compared the TRM V genes to all the germline VH in opossum and four other marsupial species (Tammar wallaby, Virginia opossum, Brush-tail possum, and Northern Brown bandicoot) and TRMV and IGHV continued to form distinct clades (Figure 9B). These results are consistent with a conclusion that, indeed, TRMV are most related to IGHV, but not sufficiently similar to extant marsupial IGHV to determine from which they might have been derived.
Figure 9. Phylogenetic analyses of TRMV to other V genes from TCR and Ig loci. A: The relationship of the V gene segments from IgH, IgL, NAR, TRA, TRD, TRB, TRD and TRM. Tree shown was generated using the neighbor joining method. Opossum TRM sequences are indicated in bold. Numbers at the nodes indicate the percent bootstrap values based on 1000 replicates. B: Phylogenetic tree showing the relationship of V gene segments from opossum TRM to IgH V gene segments from four distantly related marsupials. This analysis includes all the opossum IgH V genes which belong to three different subgroups (VH1–VH3). Available VH1 sequences from the brush-tail possum, tammar wallaby, Northern brown bandicoot and Virginia opossum were also used in the analysis. NAR-TCR was used as an outgroup. The tree was generated using the neighbor joining method with the Tajima-Nei model. Bootstrap values are indicated as in figure 9A.
In addition to the six functional TRMV gene segments located within the TRM locus, there is a single TRMV orphon gene (TRMV-OR2) located on opossum chromosome 2 in a region containing a number of flanking sequences resembling long interspersed repeat elements (LINE)(data not shown). TRM is the only of the TCR loci in the opossum for which orphon V gene segments have been found. TRMV-OR2 appears to be non-functional and has no leader peptide, but it does contain a complete V gene segment and the recombination signal sequence (RSS). TRMV-OR2 is most similar to TRMV2 and TRMV4, sharing 96 and 95% nucleotide identity, respectively. The high degree of identity between these functional gene segments and the orphon is consistent with the latter being the result of a relatively recent duplication event that, based on the flanking LINE elements may have been due to transposition [39]. TRMV-OR2 provides additional evidence for the role of retroelements in both gene translocation in marsupials. A similar translocation was reported previously for opossum MHC class I genes, where two class I loci (UB and UC) had been translocated outside the MHC region. UB and UC are similarly tightly flanked by retroelements [40,41]. Furthermore, TRMV-OR2 may also provide an additional connection between retroelements and the evolution of TCR genes themselves in marsupials. TRMVj is a variable region gene segment that appears to have been generated by retro-transposition since it is lacking an intron that all V gene segments have and is already joined to the D and J gene segments in the germline [14]. Although highly speculative, it is possible that translocated orphons such as TRMV-OR2 may have contributed to the activation of local retroelement genes to allow their co-expression when the TRM locus is actively transcribed. This would have been a necessary step in the retro-translocation events that generated TRMVj.
Is TRM present in lineages other than marsupials?
The availability of a large number of vertebrate genome sequences, with varying degrees of depth of sequence coverage, provided the opportunity to search for TRM or TRM-like genes in species other than marsupials. A search of the available genomes using the BLAST algorithm and opossum TRM sequences was unable to identify a homologue in any of the eutherian species available. This search included human, mouse, rabbit, dog, cat, cow, horse, hedgehog, elephant and armadillo. The rabbit, cat, hedgehog, elephant and armadillo are low coverage genome sequences ranging from 1.86× to 2×. While it is possible that in any given species the TRM locus was missed in the sequencing, it is unlikely that such a random gap would have been consistently present in all the eutherian genomes. Therefore we conclude that TRM is not likely present in any eutherian lineage. We also searched the current chicken and the anole lizard (Anolis carolinensis) genomes in a similar manner and were unable to detect clear TRM homologues. In the case of chicken and all the eutherian genomes, the TRD homologues were identifiable indicating that our search strategies are able to pick up this conventional TCR locus. Furthermore, we were able to identify a clear TRM homologue in the recently completed platypus genome sequence (Ornithorhynchus_anatinus-5.0) available at GenBank. Further characterization of the platypus TRM locus is ongoing and beyond the scope of this paper. Nonetheless identification of these genes in a monotreme, which are separated from marsupials and eutherians by 217 to 231 MY [15], further supports that our search strategies should be able to identify TRM homologues in other species. These results are also consistent with TRM being present early in the evolution of mammals and therefore likely lost in the eutherian lineage.
First and foremost, we have described in detail the genomic content and complexity of the T cell receptor loci for the opossum Monodelphis domestica, the first such analysis available for a marsupial. The opossum is arguably the most extensively studied marsupial species and is used as a model of human disease and development. The opossum, for example, is one of the few mammalian model organisms that develop melanoma following exposure to ultraviolet radiation providing a cancer model [42]. Additionally, opossums are a natural host and a reservoir of the causative agent of Chagas disease, Trypanosoma cruzi [43]. Like all marsupials, opossums give birth to highly altricial young also providing a model for early immune as well as other anatomical system development. Further characterization of the immune system in the opossum, and T cell immunobiology in particular, is important for better understanding of these disease and developmental models. Complete characterization of the TCR genomics in this species is one step in that direction.
In spite of detailed analyses of the opossum conventional TCR loci, the origins of TRM remain enigmatic. The current evidence support the following conclusions and model for the origin of TRM: 1) There likely was a recombination or insertion event between an IgH and TCR locus (Figure 10); 2) The TCR locus involved was most likely TRD or a TRD-like based on sequence similarity [14]. Unfortunately the highly conserved and stable organization of the TRA/D region across birds and mammals does not provide clues as to how TRD might have participated in the origins of TRM; 3) The IGH-TCR hybrid formed likely underwent a whole or partial duplication event giving rise to multiple sets of V, D, and J elements one of which remained unrearranged in the germline, the other becoming germline joined either through direct RAG mediated V(D)J recombination in the germline (left-hand path in Figure 10) or through retrotransposition (right-hand path in Figure 10). Ongoing analyses of the TRM locus in the platypus may yield further insights into these possible scenarios for the origins of this unusual TCR chain.
Figure 10. Diagram of possible scenarios for the origin of TRM. Non-homologous recombination between ancient IgH and TCR loci gave rise to a hybrid locus. A duplication event of part of the hybrid locus presumably created and additional set of V, D and J gene segments. Subsequently two possible alternatives are given for the origin of TRMVj. Left panel: A V(D)J recombination in germ cells followed by deletion of the intron located within the sequence encoding the leader peptide. Right panel: A V(D)J recombination event occurs in somatic cells, followed by transcription and splicing, resulting in a complete mRNA transcript. This mRNA was reverse transcribed to DNA by a reverse transcriptase and it was inserted back into the genome by homologous recombination. V, D and J gene segments and C regions are color-coded as in previous figures. TRMV and TRMVj are distinguished as red or yellow color, respectively. The exon encoding leader peptide is indicated with an L.
Genome analyses
The opossum whole genome assembly MonDom5 was used in this study and it is available at GenBank under the accession number AAFR00000000[23]. The location for all TCR gene segments in MonDom5 is provided in Additional file 3. For comparative purposes the current genome assemblies from human (NCBI 36), mouse (NCBI m37), cow (Btau_3.1) and chicken (WASHUC2), rabbit (RABBIT), dog (CanFam2.0), cat (CAT), horse (preEnsembl EquCab2), hedgehog (eriEur1), elephant (BROAD E1), armadillo (ARMA) and the anole lizard (AnoCar1.0) were searched for any evidence of TRM. Genomes were analyzed using BLAST assembled genomes tools [23,35].
Additional file 3. Location of opossum TRA/D, TRB, TRG and TRM gene segments. contains four tables (A-D) with the location of opossum TRA/TRD, TRB, TRG and TRM gene segments in the MonDom5 assembly.
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RNA extraction
Opossum lymphoid tissues were collected and immediately processed to extract the RNA or stored in RNAlater® (Ambion, Austin, TX) at 4°C for 24 hours and stored at -80°C for use later. Whole RNA extraction was performed using the Trizol RNA extraction protocol (Invitrogen, Carlsbad, CA). Tissue was homogenized in 1 ml of Trizol® Reagent per 100 mg until the tissue was completely dispersed. Phase separation was done using 200 μl of chloroform per 1 ml of Trizol. RNA was precipitated with 500 μl of isopropanol per 1 ml of trizol, washed with 70% ethanol and resuspended in 50 to 100 μl of DEPC water. DNase treatment to remove contaminating DNA has been performed using Ambion's kit TURBO DNA-free (Ambion, Austin, TX). Each sample was quantified using the NanoDrop ND-1000 Spectrophotometer (NanoDrop Technologies, Wilmington, DE).
Reverse transcription, PCR and sequencing
Reverse transcription-polymerase chain reactions (RT-PCR) were performed using GeneAmp RNA PCR Core Kit (Applied Biosystems, Foster City, CA). Amplifications of cDNAs were performed using AdvantageTM-HF 2 PCR (BD Biosciences, CLONTECH Laboratories, Palo Alto, California) with the conditions: 94°C for 1 minute, denaturation at 94°C for 30 seconds, annealing/extension according to the melting temperature of the primers, and a final extension period of 68°C for 5 minutes.
PCR products were cloned using TOPO TA Cloning® Kit for sequencing (Invitrogen, Carlsbad, CA). Plasmids were sequenced using BigDye Terminator Cycle Sequencing Kit v3 (Applied Biosystems, Foster City, CA) in 10 μl reactions and analyzed on an ABI Prism 3100 DNA automated sequencer (PerkinElmer Life And Analytical Sciences Inc, Wellesley, MA). Analyses of chromatograms were done using the SequencherTM 4.6 program (Gene Codes Corporation, Ann Arbor, MI).
Identification of TRV, TRD, TRJ, TRC genes
To determine the location, content and organization of the TCR genes, the whole opossum genome was searched using the BLAST algorithm. TRA, TRB, TRG, TRD and TRM were located using sequences previously isolated [28,29,38,14]. The V and J segments were located by similarity to corresponding segments from other species and by identifying the flanking conserved RSS.
Rapid amplification of 5' complementary DNA ends (5' RACE) performed on opossum thymus mRNA was used to identify novel, expressed V, D and J segments. In addition to using 5' RACE, PCR using primers that are specific for each V family were also used in RT-PCR to amplify cDNA containing VDJ recombinations that are underrepresented in the RACE PCR. Primers used to amplify the conventional TCR are complementary to the most conserved sequences of the V regions and have been paired with primers located on the C regions (Additional file 4). Sequences obtained by these means were compared with the whole opossum genome using the BLAST algorithm to identify novel TCR gene segments. This approach allowed the identification of gene segments in six possible reading frames, which also help to find gene segments located in the reverse orientation and D segments that may be used in multiple reading frames. The exon-intron organization of V regions was determined using sequences obtained by 5' RACE.
Additional file 4. List of primers used to amplify opossum TCR. this table contains the sequences of forward and reverse primers used to amplify opossum TCR.
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Determination of the exon-intron organization in the C regions was done using BLAST to compare available cDNA sequences that encode complete TCR chains to the opossum genomic sequences. Additionally, transcripts encoding the C terminal end of TCR were obtained by 3' RACE performed on thymus cDNA. Clones obtained by 3' RACE PCR were used to identify the CP, TM and CT and 3' untranslated regions (UTR) for each one of the TCR. cDNA made using the Oligo dT primer was used to perform 3' RACE PCR. The Oligo dT primer supplies a priming site for the GeneRacerTM 3' PCR primers. Sequences obtained by 3' RACE were aligned with the germline sequences to determine the location, intron-exon boundaries and splice sites of these exons.
Opossum gene segments were named following the IMGT nomenclature established for human and mouse [44]. TRV segments were numbered according to their location, from the 5' to 3' end of the locus. TRAJ segments were numbered from 3' to 5' according to a nomenclature proposed by Koop et al. [45] and followed by IMGT. The TRBD, TRBJ and TRBC found in four cassettes in the opossum are numbered according to the cassette to which they belong and the position of the cassette from 5' to 3' in the locus.
Phylogenetic analyses
Nucleotide sequences that encode from FR1 through FR3 of the V regions identified from each one of the opossum TCR loci were compared to TCR V sequences from other species retrieved from GenBank. The sequences were aligned using ClustalX [46] and BioEdit [47] programs. Phylogenetic analyses were performed using MEGA version 3.0 [48] for the distance methods (neighbor joining and minimum evolution). Confidence values were obtained from bootstrap analyses using 1000 replications.
The accession numbers for sequences used in the phylogenetic analyses are:
Figure 2
Human: A8.4: D13077; A3: X57534; A16: DQ097913; A9.2: X57531; A40: DQ097942; A10: DQ097904; A27: DQ341447; A25: DQ097923; A35: DQ097935; A22: DQ097920; A20: X70305; A34: DQ097934; A30: X58768; A41: DQ097943; A36/DV7: X61070; A17: DQ097914; A6: X58747; A24: M17661; A21: U50404; A23/DV6: D13071; A29/DV5: M17664; A1.1: X04939; A26.1: L06886; A4: M17663; A38-2/DV8: D13074; A14: S51029; D3: M23326; D1: AY357942; D2: S24406; A19: Z46641; A2: DQ097917. Mouse: A9: M33586; A17: X60319; A12.3: M38680; A6: M34200; A11: DQ340292; A13: M38102; A4: L47342; A7: X56719; A14.3: L77149; A5.4: M38681; A10: X57397; A3.1: X02967; A1: M22604; A2: X03760; A21/DV12: M94080; A15-2/DV6-2: M37599; D5: X12729; D4: M23545; TRADV16D: M16118; D2.2: M37280. Rabbit: D4: 38120; 885: M12885; D1: D26555; D5: D38121. Sheep: A622: M55622; D1S1: Z12989; D6: AJ005908; D7: AJ809501; D4: AJ005906; D2: AJ005904; D5: Z12995; D3S2: Z12996; A35: U78035. Cow: 014: D90014; 013: D90013; 015: D90015; 011: D90011; 012: D90012; 017: D90017; D113: D16113; 016: D90016; D116: D16116. Chicken: 611: GDU04611; 612: GDU04612; 613: GDU04613.
Figure 5
Human: B11.3: X58797; B7.2: U07975; B12.1: X07224; B14: X06154; B17: U03115; B2: M64351; B27: U66061; B25.1: L27610; B13: U03115; B4.3: X58812; B21.1: L27608; B23: L27614; B5: X61439; B9: M27380; B3.1: U07977; B15: U03115; B24.1: L27612; B10.3: U17047; B28: U08314; B6.9: X61447; B19: U48260; B30: L06893; B20.1: X72719; B29.1: M13847. Mouse: B14: AE000664; B16: L29434; B3: AE000663; B21: X16691; B26: K02548; B23: X59150; B24: M61184; B12: M30881; B2: AE000663; B4: X56725; B17: AE000664; B29: X00696; B10: X16694; B13.3: M15616; B19: AJ249821; B31: X03277; B30: X16695; B20: M11859; B1: X01642. Rabbit: B8: BAA04245; B11: BAA04248; B9: BAA04246; B7S1: BAA04241; B1: AAA31472; B5: BAA04239; B10: BAA04247; B2: M13895; B6: BAA04240. Sheep: B6S1: AAB88431; B8S1: AAB88433; B10S1: AAB88434; B1S5: AAB88425; B7S1: AAB88432; B22S1: AAB88440; B3S1: AAB88427; B13S1: PQ0068; B15S1: AAB88438; B12S1: AAB88435; B17S1: AAB88439; B2S1: AAB88426; B4S1: AAB88430. Cow: B126: PQ0062; B129: PQ0065; B125: PQ0061; B122: JQ0473; B123: PQ0060; B124: PQ0059. Chicken: B1S1: B36198; B2S2: AAA62753.
Figure 7
Human: G2: M13429; G3: S60779; G4: S60780; G2: M27335. Mouse: G3: AF037352; G6: M13338; G4: M13336; G7: AF037352; G1: Z22847. Rabbit: G1S2: D38137; G1S3: D38138; G1S1: D38135; G1S4: D38139; G2S1: D38142. Sheep: G6S1: Z13007; G2S1: Z12999; G2S2: Z13000; G2S3: Z13001; G2S4: Z13002; G5S1: Z13005; G5S2: Z13006; G1S1: Z12998; G3S1: Z13003; G4S1: Z13004. Cow: G6: AY560834; G1S1: D16119; G1S3: D16131; G5S11: D16126; G5S7: D16130; G5S6: D16129; G5S16: D16133; G3S1: U73186; G3S2: U73187; G4S1: U73188. Platypus: V3.1: AAY82120; V3.2: AAY82094; V1.1: AAY82100; V1.2: AAY82093; V1.3: AAY82109; V2.2: AAY82110; V2.3: AAY82114; V2.1: AAY82119. Chicken: G3S8: U78235; G3S4: U78231; G3S3: U78230; G2S7: U78225; G2S8: U78226; G2S9: U78227; G1S4: U78212; G1S5: U78213; G1S3: U78210; G1S8: U78216.
Figure 9A
IgH: Hsheep80: U80145; Hmouse21: M27021; Hhuman47: X64147; Maxolot43: L20243; Hmouse69: K01569; Hhuman14: X05714; Mxenopus84: M20484; Hmouse70: M21470; Hhuman00: DQ454900; HHlama75: AY544575; HHlama33: AY342133; HHlama32: AJ629032; HHlama86: AF441486; Hpig94: U15194; Hhuman62: U80162; Hopossum13: AF012113; Hopossum24: AF012124; Hhuman86: M99686; Hmouse65: M25465; Hmouse85: M31285; Hmouse01: X03301; Mchicken48: M30348; Mzebrafish80: AF281480; Mcatfish60: DQ230560; Mtrout03: DQ831803; Mrockcod55: AF303555; Hwolffish86: AY188786; Hsturgeon61: DQ257661; Wnurseshark50: U51450; Wlungfish27: AF437727; Mnurseshark51: M92851; Hhornshark49: X13449; Hsandbarshark57: AY548357. NAR-TCR: TnarsharK88: DQ022688; Tnarshark10: DQ022710; Tnarshark06: DQ022706. NAR: Nwobbegong92: AF336092; Nwobbegong94: AF336094; Nnurseshar03: AF447103; Nnurseshar81: AY114781. IgL: Khuman92: Y08392; Khuman98: DQ915098; Ksheep10: X54110; Krabbit53: AF211353; Krat09: U39609; Khorse11: X75611; Kopossum08: AY074408; Lnurseshark44: GCU15144; Khamster65: U17165; Khuman98: AY320598; Lmouse88: DQ986488; Lray46: AB062646; Lseabream69: EF555069; Lsturgeon42: AJ387842; Lsalmon17: AF273017; Lseabass79: AJ291779; Ltrout60: X65260; Lhuman77: AF216777; Lopossum81: AF049781; Lchicken67: S65967; Lxenopus78: L76578; Lhuman69: Z73669. TRG: TGhuman29: M13429; TGplatypus21: DQ011321; TAmouse98: M34198; TArabbit85: M12885; TGhuman79: S60779; TGcow86: U73186; TGrabbit42: D38142; TGcow88: U73188. TRA/D: TAsalmon97: EF467297; TAsalmon05: EF466505; TAfugu52: AY198352; TAselfish71: AY198371; TAselfish49: AY198349; TAcod44: AJ133844; TAfugu95: AB222395; TDhalibut71: AB076071; TDfugu79: AB222479; TAzebrafish50: AL592550; TADhuman96: Z14996; TAsheep35: U78035; TDsheep03: AJ005903. TRB: TBtrout23: OSU18123; TBseabream37: AM490437; TBfugu32: AB222432; TBselfish13: AF324813; TBopossum8.1: XM_001363148; TBmouse82: DQ983582; TBsheep11: AF030011; TBrabbit19: D17419; TBhuman04: M27904; TBsheep17: AF030017; TBcow29: D90129; TBmouse16: M15616.
Figure 9B
Gray short-tailed opossum: IGHV1-1: AAC48826; IGHV1-6: AAC48820; IGHV1-11: AAC48816; IGHV1-16: AAC48836; IGHV1-23: AAC48846; IGHV2-1: AAC48849. Remaining germline sequences are available at AAFR00000000. Brush-tail possum: 70: AAL87470; 71: AAL87471; 72: AAL87472; 73: AAL87473; 74: AAL87474; 76: AAL87476; 77: AAL87477; 78: AAL87478; 79: AAL87479; 91: AAD41691; 92: AAD41692; 41: AAT40441; 40: AAT40440; 44: AAT40444; 43: AAT40443; 42: AAT40442. Virginia opossum: P83 is an unpublished sequence kindly provided by Dr. R. Riblet. Tammar Wallaby: sequences are unpublished but available upon request. Northern Brown Bandicoot: Bandicoot58: AY586158. Shark NTCR: AAY98815.
Dot plot analyses
Comparisons of the genomic sequence were performed using the program Spin from the Staden Package [49]. Dot matrix plots were generated to determine degrees of similarity among cassettes for the TRB locus. The sequence analyzed includes the four TRB cassettes with coding and non-coding sequence from 3kb upstream of the most 5' TRBD (TRBD1) segment to the most 3' TRBC (TRBC4) segment, which comprises 51 kb. Although not shown, similar comparisons were performed for the other TCR loci.
TCR: T cell receptor. TRA: T cell receptor alpha. TRB: T cell receptor beta. TRG: T cell receptor gamma. TRD: T cell receptor delta. TRM: T cell receptor mu. Ig: immunoglobulin. V: variable gene segment. D: Diversity gene segment. J: Joining gene segment. C: constant region. Tm: transmembrane region. Cp: connecting peptide. Ct: cytoplasmic region. RSS: recombination signal sequence. MHC: major histocompatibility complex.
Authors' contributions
ZEP, MLB, and RDM conceived of the study, participated in its design, and drafted the manuscript. ZEP, JH, AML, JT, and AS generated the data and performed the sequencing reactions. ZEP performed the data analyses. All authors have read and approved the final manuscript.
This work was supported by funding from the National Institutes of Health Initiative for Maximizing Student Diversity (ZEP, JT) and Institutional Development Award (IDeA) programs (MLB, JH), the Robert C. McNair program (AML) and the National Science Foundation (RDM).
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Original Gravity Public House 2 Year Anniversary Party
- When
- Sat Jul 19, 2014
- Where
- Original Gravity Public House
- Time
- 12pm
- Tags
- Bars, Beer Houses & Brewers
DescriptionJoin us for a truly special day of celebration including extremely special kegs, bottle releases, food specials, limited edition anniversary t-shirts and music on our back patio. We will also be unveiling a few more surprises so check back often for more details TBA!
We are in the midst of an expansion which will provide an estimated additional 50 seats indoors, a second bar with 10 more taps, a slightly updated food menu and a much needed extra restroom. This space should be open by the party so consider this in some ways our grand re-opening.


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Originally written on Mets360.com | Last updated 8/27/13
By now, we all know the terrible news about Matt Harvey's partially torn UCL, which might require surgery. If Harvey would need to undergo Tommy John surgery, he would miss mot (if not all) of the 2014 campaign. The news was heartbreaking to all of us. It was the first time in a while that I didn't have words to describe my feelings about what I was reading, hearing, seeing. Never before has it been so hard to try to put something like this into perspective, and it still isn't. But last night during the Mets game, there was a moment that put it all together for me and really gave this sad story a promising future. For those who have not seen this yet, be prepared to be a bit depressed for a moment. Credit to this goes to JewishDoggy on Reddit: Now there are two ways to look at this and both would be appropriate. The first would to feel that the world is coming to an end and everything that Sandy Alderson and the Mets have been working towards has been flushed down the drain. ...
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a lovely dough, perfect to make with young children, such a short resting time means they will soon be eating their creation.
Quick Pizza Dough
From EatingWell: November/December 1996, July/August 1996
In less time than you can drive to the store and back, this homemade pizza dough is ready.
6 Reviews for Quick Pizza Dough
Tried this one night when I was running late and didn't have time to rise my normal pizza dough - this recipe was a revelation! It was so quick! I just whizzed it all together as per the instructions, and when I went to knead it the dough was soft and pliable, a bit sticky but a floured board was sufficient. The dough rolled out very easily and went a long way and the finished result was thin and crispy. Will make again!
The recipe doesn't say what temp and how long to bake this crust at...I guess I'll just keep my eye on it and take the pizza out when it looks done...But I'm curious if anyone else opts to toast the crust a little before putting on the toppings. I usually do that and it tends to result in a crunchier, sturdier crust. I hate soggy crust!
I subbed WW pastry flour and regular yeast (let sit for the same amount of time- 15 minutes). Added fresh rosemary and Italian seasoning. It made a flavorful, crisp and slightly chewy thin crust- awesome! So fast and simple. Worked great for my grilled pizza recipe, and turned out 4, 1/8-inch thickness pizzas, about 8-10 inches in diameter, each.
This was fast and easy! The dough was good. It was light and fluffy, not chewy like I prefer but for fast crust that doesn't need to raise, pretty good! We will definitely be making this again.


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This round black dolly is the easiest way to transport your BRUTE containers from 20 Gal to 55 Gal in size, holding up to 250 lb at one time. The all-plastic construction resists rusting, chipping and denting, and the heavy-duty swivel wheels make moving even heavy loads a snap. Swivel castes offer better mobility through the workspace. Heavy-duty 3" casters. Includes one dolly. High-Density Polyethylene (HDPE). Holds BRUTE Container Models:2630, 2632, 2643 and 2655. Size: 6 5/8" H x 18 1/4" Dia.
Additional Information


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In line with these portfolio decisions, Sunesis is reducing operating expenses primarily in the research and general & administrative areas. As part of the reorganization, the company is eliminating a total of 35 full-time employee positions, and will now have 108 employees. Sunesis expects that this realignment of personnel, operations and programs will reduce annual expenses by more than $10 million below its planned levels for the next several years.
"With the talent, focus and commitment of the Sunesis team, we have the opportunity to generate transforming clinical data in the coming quarters. By proactively undertaking the realignment, we can focus on the execution of our revised operating plan without needing to raise additional funds or entering into a corporate partnership in 2008," said Daniel Swisher, Chief Executive Officer and President of Sunesis. "The workforce reductions we're announcing today are difficult. We are deeply grateful for the dedication and contributions of the employees who will be leaving Sunesis and wish them the very best in all their future endeavors."
The company will be providing severance and career transition
assistance to those employees directly affected by the restructuring. As a
result of the restructuring plan, Sunesis estimates that it will record a
one-time restructuring charge in the third quarter of 2007 of between
approximately $1.0-1.2 million for personnel costs and approximately
$0.5-0.8 million for facilities-related and other costs. Sunesis estimates
that the total amount of the restructuring charge will be between $1.5-2.0
million. The cash portion of this restructuring charge will be
approximately $1.0-1.2 million. As of June 30, 2007, Sunesis had cash, cash
equivalents and marketable securities of $65.2 million. The company now
anticipates that cash used in operating activities in 2007 will be le
|SOURCE Sunesis Pharmaceuticals, Inc.
Copyright©2007 PR Newswire.
All rights reserved


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November 17, 2012 News
SKUSA SuperNationals XVI - Friday Report
The 2012 edition of the Superkarts! USA SuperNationals began two days ago outside the Rio All-Suite Hotel & Casino in Las Vegas as nearly 500 drivers made their way onto the temporary circuit for the first time. Friday (November 17) marked the first day of three where results on track matter - with qualifying and the opening round of heat races taking place.
The day commenced under overcast and cloudy skies on a track that is most-favourable to all the racers here, touting it as one of the best tracks ever for the SKUSA SuperNationals.
Following a seven minute warm-up run for the morning classes (S4, S5, KZ2, and TaG Senior), it was time for the qualifying runs to determine the grids for the three heat races to come for each class.
First up was the large S4 class, with the contingent broken up into two groups. In Group 2, the initial one's on-track, Willy Musgrave (Tony Kart) was the early driver to watch virtually the entire session, securing the provisional pole via a fast lap of 45.758. Canadian Don McGregor (Italkart) and Chris Perreault (Vemme) were 15th and 24th respectively. Group 1 wasted little time going quicker. In the end, defending champion Bonnier Moulton (Tony Kart) posted a lap of 45.353 to secure the pole. The 16-time SuperNats competitor and two-time winner edged out Brian Fisher (DR Kart) by just two-thousandths of a second. Championship contender Eddie Olpin (Kosmic) ended up third over SummerNationals winner Trevor McAlister (Praga) and defending King of the Streets winner Jordon Musser (Birel). Musgraves time slotted him sixth overall.
Next up in the morning qualifying runs were the youngest Stock Moto drivers, S5 Junior. While Patricio O'Ward (GP) led the way early on, it was Carter Williams who proved quickest in the second half of the session, earning the pole with a 46.169-second fast lap. O'Ward held on for second, just ahead of Andrew Evans (Kosmic), Jim McKinney (KGB) - the current SKUSA Pro Tour points leader - and Kiwi driver Marcus Armstrong (GP), making his first shifterkart and SuperNats start. Canadian Nelson Learn (GP) was 25th.
As expected, the headline KZ2 class fight for the pole and the Oakley Lead Foot award proved entertaining to watch. Featuring a mix of the best from USA and abroad going head-to-head, second generation driver Max Verstappen (son of former Formula One driver Jos Verstappen) proved the racer to beat, securing the Oakley award and pole with a quick lap of 43.586 aboard his CRG machine. Marco Ardigo (Tony Kart), a two-time former champion, was P2, followed by Patrik Hajek (Praga), Rick Dreezen (Tony Kart) and Ben Hanley (ART GP). Seven different brands were represented in the top-10, including defending race winner Anthony Abbasse (SodiKart), the Energy of American Gary Carlton in seventh and former KF2 champion Armand Convers (Kosmic). Philip Orcic (Zanardi) led the Canadian contingent in 17th, ahead of Zach Meyer (GP) in 22nd, Michael Vincec (Vemme) in 24th, and Nico Fattore (GP) in 27th.
Completing the morning qualifying sessions was the huge TaG Senior class, which was split into two groups. Stepanova Nekeel (LH) was on-top throughout the Group two run, posting a lap of 46.579 to lead the group. Chad Campbell (GP) topped the Canadians in 12th, leading Alejandro Liverant (Praga) in 17th, Jamie McAllister (Italkart) in 20th, Devon Sandeen (Vemme) in 34th, and Marcus Brodie (Vemme) in 38th. As expected, the Group 1 racers were quick to take over the top of the timing monitors, when they rolled on-track in Las Vegas. Charles Leclerc (ART GP), who was quickest in practice on Thursday, in the end captured the pole with a time of 46.058, edging the young Dennis Olsen (Energy), Canadian Michael Valiante (Italkart), Austin Elliott (Top Kart), and Aussie David Sera (Arrow). Other Canadians in Group 1 included Scott Hargrove (Italkart) in 15th, Luke Chudleigh (Maranello) in 21st, Remo Ruscitti (Italkart) in 27th, Adam Isman (Italkart) in 31st, and Cory Cacciavillani (Kosmic in 44th.
With the chase for one quick lap in the books, it was time to get down to some racing via the opening Heat races for the morning contingent.
The chase for S4 supremacy got underway with Group A vs. Group D going head-to-head. When the green flag waved to get the 12-lap affair underway, Moulton made the most of his P1 position to hold off P2 starter McAlister. With the lead duo repeatedly trading fastest race laps, it was apparent that either one could take home the victory. McAlister ultimately made his move on lap eight, holding the advantage for a narrow Heat 1 win. Jim Inglebright (GP) was the third driver to the stripe, leading Eduardo Martins (Tony Kart), and Marks, who took over fifth place with a late race pass.
Immediately following the checkered flag waving, Group B and C of S4 were on-course for their 12-lap duel. Like the opening run, the polesitter Fisher made the most of his starting position to hold off the charges from behind through the first few corners. This ultimately proved to be the deciding factor in the race, as a duel between Olpin and Musgrave for second place allowed for Fisher to steadily pull away. The fight for the runner-up position lasted the majority of the race, eventually going in Olpin's favor. Coy Dayton (Tony Kart) and Rob Logan (CRG) completed the top-five. Canadians McGregor and Perreault were ninth and 26th respectively.
When the green flag waved a short-time later for S5's first Heat race, the fans on hand witnessed their first opening lap pass of the day, as Williams immediately relinquished the lead to Andrew Evans. In fact, in what was pre-cursor to difficult race (finishing sixth), he was down a further position by the end of the lap one. Evans run out front, didn't last, as O'Ward soon found a way past. Behind the lead pair, the action was just as entertaining to watch, with Erik Nelson (Kosmic), Axel Matus (GP), Carter, and Micah Hendricks (Praga) in close formation. While Evans closed in the final stages, O'Ward held on for the win. Nelson completed the top-three, leading Matus and Hendricks to the stripe. For the championship leader McKinney, a slow green light to begin the race led to a flooded carburetor as the race began and he was hit from behind, collecting a handful of karts as well to end the first race on the sidelines. Learn was 15th at the stripe
The KZ2 standouts were the next group to go racing. Verstappen enjoyed a solid launch when the green flag waved, leading Hajek and the rest of the field through the opening sequence of corners. Just as it appeared Verstappen was headed for a straight forward victory, he mysteriously fell back to third behind Hajek and Ardigo, and come under attack from Abbasse. The Dutch up-and-comer, however, remained calm, promptly string some very fast laps, work his way back into second by lap eight. Closing the gap on Hajek, Verstappen passed for the lead and the win, albeit with via some contact, a few laps before the checkered flag waved. Ardigo followed the pair across the start/finish, several kart lengths ahead of Abbasse. Dreezen completed the top-five via a last lap pass of Carlton. Meyer was the top Canadian, 14th, ahead of Fattore in 24th, Orcic, who was caught out in an early race incident, in 28th, and Vincec, who failed to complete a lap after contact, in 32nd.
The final races of the morning classes featured the highly regarded TaG Senior, with Heat A versus D first on tap. Leclerc led the large field to green, and promptly held the P1 position, as Sera slotted into second. With Sera immediately coming under contact from Elliott, the lead steadily grew. In fact, the fight for second rapidly became a multi-kart affair, with Olin Galli (Energy) and Tommy Andersen (Kosmic) joining the fray. While Sera was ultimately able to separate himself from the dice, he never got within striking distance of the leader, giving Leclerc an easy opening Heat win. Behind the duo, order was only decided with the waving of the checkered flag, with Galli taking home third over Andersen and Elliott. Chudleigh earned a strong top-10 with an eighth place finish, while Campbell, Isman, and McAllister came home in 25th, 29th, and 30th respectively.
After two failed starts, the Heat B vs. C TaG Senior encounter witnessed 2011 Pro Tour TaG Junior champion Jake Craig (Kosmic) getting an ideal launch to take over the top spot ahead of Olsen and Valiante. Subsequent contact between the chasing pair allowed Kiel Spaulding (FA Kart) and Andre Nicastro (Italkart) to move into the top-three. That, however, proved to be just the start of the action, as the race progressed, so did the intensity of the fighting among the lead pack. With plenty of bumping and banging going on, the order repeatedly changed, particularly in the final few tours. Spaulding in the end emerged with victory over Olsen. Louie Pagano (Birel) enjoyed a great late race charge, beating Nicastro and Craig to the checkered flag. Valiante ultimately took the checkered flag in sixth, just in front of his teammate Hargrove. Ruscitti was the next Canadian in 15th, ahead of Cacciavillani in 23rd, Sandeen in 25th, Brodie in 30th, and Liverant in 33rd.
After the lunch break and warm-up runs, it was time for the afternoon classes (TaG Cadet, TaG Junior, TaG Master, S2, and S1) to go after the pole positions.
With a huge field on hand, the TaG Cadet class was broken into two groups. In the opening Group 2 outing, Max Hewitt and Dante Yu repeated swapped fastest laps, before Hewitt secured the provisional pole via a time of 52.607. Canadian Griffin Dowler (Energy) was 19th. As expected, Group 1 proved even quicker, with several racers trading the top spot on the timing monitors in the first part of the run. Canadian Antonio Serravalle (Tony Kart) in the end proved quickest of the bunch, circulating in 51.846 to better Hunter Kelly by just five-hundreths of a second - leaving the Pro Tour championship contender that short from gaining ten bonus points. East coast drivers David Malukas (Birel), Zach Holden (Top Kart), and Anthony Gangi Jr. (Tony Kart) rounded out the fast-five. Canadian Roman DeAngelis (Kosmic) was 16th.
TaG Junior was next up, and it too was divided into two groups due to its large size. Parker Thompson (Energy) proved to be the driver to beat in Group 2, posting a lap of 47.085. Fellow Canadians Jordan Redlin (Zanardi) was 14th, Gianfranco Mazzaferro (Zanardi) 25th, Tyler Ripani (LH) 26th, and Ethan McDade (Italkart) 31st. The Canadian's run as the qualifying leader was short-lived, as the Group 1 leaders were quick to make their mark. After what was a back and forth affair featuring several drivers, defending race winner George Russell (OK1) secured the pole via a lap of 46.636 seconds. Canadian Lance Stroll (Zanardi) was next on the timesheets, followed by Yurik Carvalho (Italkart), Davey Manthei Jr. (Kosmic), and Dalton Sargeant (Tony Kart). Canadian Zachary Claman DeMelo (CRG) Devlin DeFrancesco (CRG) were 22nd and 29th.
As close as the young racers had been in qualifying the elder TaG Master fight for the pole was even more impressive. Going right down to the wire, Pro Tour point leader Scott Falcone (Arrow) came away with top honors, thanks to a fast lap of 47.241, mere hundredths of a second faster than Larry Fraser (Tony Kart). Two-time defending champion Leonardo Nienkotter (Kosmic) was P3, ahead of Robby Mott (Corsa) and Paul Bonilla (Arrow).
The S2 Semi-Pro Stock Moto class did not disappoint as well when it took to the track for qualifying, with several racers in contention for the pull. The P1 position ultimately went to SpringNationals winner Billy Musgrave (Tony Kart) following a lap of 44.827. Canadian Remo Ruscitti (Italkart) and Austin Schimmel (GP) completed the top-three, by bettering Connor Wagner (FA Kart) and Pro Tour point leader Sabre Cook (CRG). Scott Hargrove (Italkart) was the next Canadian in ninth, ahead of Adam Dowler (Energy) in 34th, and Austin King (PCR) in 36th.
Concluding qualifying at the 16th edition of the SKUSA SuperNationals was S1. In what was a strategic affair, many of the expected front-runners elected to sit in pit lane in the early parts of the session. When they did hit the track, the times at the top of the scoring charts rapidly dropped, with several racers taking a turn on top. European KZ2 champion Simas Juodvirsis (Energy) proved to be the fastest man, posting a lap of 44.917 to edge Kiwi driver and defending Pro Tour champion Daniel Bray (GP) for the pole by just 43-thousandhts of a second. Italian karting legend Alessandro Manetti (Praga) was third in his first start for the S1 category while NorCal drivers Nic LeDuc (CRG) and Jason Toft completed the fast-five.
Following the traditional Sunset break and the lights coming on, it was time to go racing once again at the Rio.
First up was TaG Cadet Heat A versus D, and Serravalle and Holden leading the field to the green. While Serravalle got away cleanly to hold off a hard charging Neil Verhagen (Arrow), Holden dropped down to seventh. The man to watch early on, however, was Gangi Jr., working his way into the lead by lap four. This proved to be the decisive move, as Serravalle was forced to duel Luiz Branquinho (Tony Kart) and Verhagen, allowing Gangi Jr. to build an advantage. Serravalle fought back, retaking the second spot and challenged Gangi to the final corner for the win. At the line, it was Gangi over Serravalle. Verhagen was third, leading DeAngelis, who enjoyed a superb drive that culminated in a last lap pass for fourth over Holden. Dowler was 25th.
The TaG Cadet Heat B versus C was next, and it proved great fun to watch. Right from the get go, the battle for the win was on, with polesitter Kelly and Leonardo Stoia (PCR) swapping the top spot several times in the opening tour. As entertaining as that was, Brandon Lemke (Merlin) generating a lot of interest, charging into the top-three by lap five. This subsequently resulted in a superb duel with Santiago Terife (Tony Kart). Despite fighting for position from corner to corner, the duo found a way past Stoia making a battle for second, albeit well behind Hunter. In the end, Hunter scored an easy victory over Lemke and Terife. Rasmus Lindh (Kosmic) came home fourth, thanks to a last lap pass of Jake Drew (CRG).
When the TaG Junior Heat A versus D race got underway, all eyes were on the polesitter Russell, and he did not disappoint. Making the most of disastrous first few corners for fellow front row starter Manthei., that him witnessed him fall to 12th, he built a comfortable early advantage. It was not to last though, as Dalton Sargeant (Tony Kart) not only worked his way into second ahead of Pedro Cardoso and Vitor Batista, but steadily reduced the gap to the leader. In the end there wasn't enough time, as Russell scored a flag-to-flag win over Sargeant. Batista, running alone most of the race was third at the flag, while Kurt Kostecki came home fourth via a strong late race charge. Nick Ramirez completed the top-five a pass in the final few corners. Ripani was 20th, while Thompson, was involved in a first lap crash was classified in 37th.
The second TaG Junior race (Heat b vs. C) proved to be a dramatic affair. Things commenced with the front-row of Stroll and Carvalho being sent back to row two after two failed launches. While this allowed Pro Tour point leader Luke Selliken (Kosmic) and Marino Sato lead the field to the green, Stroll still got the best launch, immediate vaulting into the led. Behind Stroll and Selliken, Colton Herta (DR Kart) were also on a charge, rapidly slotting into fourth, just in front of Thomas Schou. From that point forward the foursome battled for positions, with Selliken ultimately getting the best of Stroll, and Schou finding a way past Herta. While these battles garnered most of the attention, some late race drama occurred in the fight for fifth. Mauro Auricchio, having been among the fastest drivers in the closing stages, and Pro Tour title contender Brenden Baker (CRG) fought tooth and nail, subsequently resulting in contact that not only sent both down the order, but also allowed Carvalho to take home fifth. Claman DeMelo and DeFrancesco earned top-10s in seventh and 10th respectively, while Mazzaferro was 19th, Redlin 26th, and McDade 28th, after a first lap incident.
The TaG Master Heat 1 likewise was an exciting race. In untypical fashion, the start proved to chaotic, with lead duo of Falcone and Fraser getting involved in a first lap incident, giving Nienkotter the lead over Mott. With Billy Cleavelin (CRG) rapidly joining the fight care of some very quick laps, the trio regularly looked to find a way past one another. Not to be out done, title contender Paul Bonilla (Arrow) and Jim Russell Jr. (Parolin) squared off for fourth place. Only decided at the stripe, Mott scored a narrow win over Cleavelin and Nienkotter, while Bonilla and Russell Jr. completed the top-five.
When the S2 karts rolled on-course for their first Heat, it was anyone's guess how things would play out. Musgrave made the most of his P1 starting position holding the top spot through turn one. Behind the leader Sabre Cook and Connor Wagner got the best jump, vaulting into second and third as front-row starter Ruscitti fell back to fifth. Just as the field was beginning to space out, the Red flag waved for Dalton Egger (Tony Kart) having heavy contact with the turn four barriers. After seeing to the racer and ensuring his safety the race resumed with six laps to go. On the single-file restart, Cook tried to make a move for the lead, but was held back by Musgrave. That proved decisive, as he subsequently held the top-spot to the checkered flag. Behind the duo, the restart greatly benefited Scott Hargrove, as the Canadian jumped all the way up to third, holding the position to the finish, ahead of Wagner and Milan Dontje (Energy). At the line, King was 28th, Dowler 30th, and Ruscitti 39th, having retired six laps in.
The first day of racing concluded with S1 drivers battling. LeDuc was the racer on the move when the green flag waved, not only taking over the lead, but building a sizeable gap on the polesitter Juodvirsis. Initially it appeared as the advantage may last, but as the race progressed, Juodvirsis kart hooked up, steadily getting closer and closer to the leader. On lap 10, the European standout out-braked LeDuc for first and ultimately the win. Behind the duo, Bray had an eventful race. Falling back to sixth at the start, he slowly recovered, working his way into the top-five in the closing stages, before passing for fourth and third in the final two laps. Championship leader Fritz Leesmann (CRG) likewise was forced to work his way from aways back in the race, in the end taking fourth place at the stripe. Toft came home in fifth, having run as high as third early on. Wimsett, the second half of the championship chase, fell back to nearly last and worked his way up to 10th, gaining five points toward his total.
EKN Trackside Live continues with rounds two and three of the heat races and the Last Chance Qualifiers for the S4, TaG Senior, TaG Junior and TaG Cadet categories. Tune in to
at 8:30am PST for the start of the morning categories with the afternoon groups beginning after that. Visit the
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You don't have to be a geopolitical wonk to know that Iranian President Mahmoud Ahmadinejad is no fan of Israel. So you can imagine he wasn't too happy to find a Jewish star hiding in plain sight. Yep, that one in the photo from Google Earth, which reminds me of this.
From the JPost:
Did Israeli prankster architects sneak a Star of David onto the roof of the Teheran airport, or is the controversy in Iran over a Google Earth revelation much ado about nothing?
The six-pointed star was discovered by an eagle-eyed Google Earth user recently, over three decades after the building that houses the national airline of the Islamic Republic was constructed by Israeli engineers.
Israel and the Shah's Iran maintained good ties until the Islamic Revolution of 1979 ended the relationship. Before 1979, Israel brokered arms deals with Iran, and there were regular flights between Teheran and Tel Aviv.
Once the existence of the Star of David was reported in Iranian media, government officials called for the immediate removal of the apparently offensive Jewish symbol.
Obviously, the Elders of Zion had their hand in this one.
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text:
Oct 29 (Reuters) - TOYOTA INDUSTRIES CORP CONSOLIDATED FINANCIAL HIGHLIGHTS (in billions of yen unless specified) 6 months ended 6 months ended Full year to Full year ended Sep 30, 2010 Sep 30, 2009 Mar 31, 2011 Mar 31, 2010 LATEST YEAR-AGO COMPANY YEAR-AGO H1 RESULTS H1 RESULTS FORECAST RESULTS Sales 729.81 627.56 1.48 trln (+16.3 pct) (-32.4 pct) (+7.4%) Operating prft 36.21 loss 2.80 prft 65.00 (+195.4%) Recurring 38.39 4.27 70.00 (+800.1 pct) (-89.4 pct) (+120.4%) Net ...
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