text:
Book your room at Ramada Plaza Fort Lauderdale
Standard Room, 1 King Bed, Non SmokingMax Occupancy: 2 guests (up to 1 child)
Room, 2 Double Beds, Non SmokingMax Occupancy: 4 guests (up to 3 children)
Room, 2 Double Beds, Non SmokingMax Occupancy: 4 guests (up to 3 children)
Standard Room, 1 King Bed, AccessibleMax Occupancy: 2 guests (up to 1 child)
Get an overview of this hotelHotel in Fort Lauderdale with free breakfast and restaurant
Situated in Fort Lauderdale, this hotel is 1.6 mi (2.6 km) from Lakes Mall and 2.7 mi (4.4 km) from Shoppes of Oakland Forest. Fort Lauderdale Stadium and Swap Shop are also within 6 mi (10 km).
Hotel Features
A restaurant, an outdoor pool, and a fitness center are available at this smoke-free hotel. Free continental breakfast and free self parking are also provided. Other amenities include a poolside bar, a spa tub, and coffee/tea in the lobby.
Room Amenities
All 269 rooms provide room service, coffee makers, and cable TV. Hair dryers and ironing boards are also available to guests.Information missing or incorrect? Tell us!
Hotel Amenities
Hotel Amenities
In addition to an outdoor pool, Ramada Plaza Fort Lauderdale provides a spa tub and a fitness center. The hotel offers a restaurant. A poolside bar is on site where guests can unwind with a drink. Guests can enjoy a complimentary breakfast each morning.
2 meeting rooms are available. This Fort Lauderdale hotel also offers tour/ticket assistance, an arcade/game room, and gift shops/newsstands. Onsite self parking is complimentary.
Ramada Plaza Fort Lauderdale is a smoke-free property.
- 24-hour front desk
- Arcade/game room
- ATM/banking
- Coffee/tea in lobby
- Free newspapers in lobby
- Dry cleaning/laundry service
- Elevator/lift
- Fitness facilities
- Free breakfast
- Free self parking
- Gift shops or newsstand
- Hair salon
- Laundry facilities
- Number of meeting rooms - 2
- Outdoor pool
- Poolside bar
- Restaurant
- Smoke-free property
- Spa tub
- Tennis on site
- Total number of rooms - 269
- Tours/ticket assistance
Family Friendly Amenities
- Arcade/game room
- Laundry facilities
- Outdoor pool
- Rollaway/extra beds available
Free self parking
Room Amenities
- Air conditioning
- Cable TV service
- Coffee/tea maker
- Hair dryer
- Iron/ironing board
- Rollaway/extra beds available
- Room service (limited hours)
Where to Eat
A complimentary continental breakfast is served each morning between 7 AM and 11 AM.
Beau Alexanders Restauran - This restaurant serves breakfast, lunch, and dinner.
Room service (during limited hours) is available.
Nearby Things to Do
An outdoor pool and a spa tub are on site. Other recreational amenities include a fitness center.
- Golfing nearby
To make requests for specific accessibility needs, complete the "Special or Accessibility Requests" section of "Trip Preferences" when you book your hotel.
- Accessible bathroom
- Accessible parking
- In-room accessibility
Hotel Policies
Check-in time starts at 3 PM
Minimum check-in age is 21
Check-out time is noon
Payment types
Children and extra beds
- Children are welcome.
- Rollaway/extra beds are available.
You need to know
Extra-person charges may apply and vary depending on hotel policy.
Government-issued photo identification and a credit card are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
- Deposit: USD 50.00 per night
We should mention
A resort fee is included in the total price displayed.
Breakfast for 2
Book a stay in any room or rate category and receive a complimentary continental breakfast for 2 guests per room, per day. Valid for all room types and rate plans. Details at check-in. Restrictions may apply.


text:
Originally written on Fox Sports | Last updated 3/20/13
When Baylor guard Gary Franklin first saw the NIT bracket, the California native got excited. He initially thought the Bears' matchup against Long Beach State meant a chance to go play only about a half-hour from home. The 49ers (19-13), whose roster includes two of Franklin's teammates from a high school championship team, will instead be in Texas on Wednesday night for a first-round game. ''The only disappointing thing for Gary is he wanted the game back home,'' Bears coach Scott Drew said with a smile, adding that they were able to get an early scouting report from him. Baylor (18-14) is back in the NIT four years after making it to Madison Square Garden, then losing to Penn State in the championship game. The Bears, who since their NIT runner-up finish in 2009 have made it to two NCAA regional finals, had bigger aspirations this season than a No. 2 seed in the NIT. ''You want to be in the Big Dance. At the same time, it's something you can't control as of right now,'' said ...
Comments are closed.
What do you think?To leave a comment, you can:
College Basketball News
Delivered to your inbox
You'll also receive Yardbarker's daily Top 10, featuring the best sports stories from around the web. Customize your newsletter to get articles on your favorite sports and teams. And the best part? It's free!
Follow us on Twitter
|Best of Yardbarker
|NFL News
|MLB News
Today's Best Stuff
For Bloggers
Join the Yardbarker Network for more promotion, traffic, and money.
Company Info
What is Yardbarker?
Yardbarker is the largest network of sports blogs and pro athlete blogs on the web. This site is the hub of the Yardbarker Network, where our editors and algorithms curate the best sports content from our network and beyond.
|© 2015 Yardbarker, Inc. All rights reserved. Use of this website (including any and all parts and components) constitutes your acceptance of these Terms of Use and Privacy Policy. (What's New?)

subset: commoncrawl


text:
After being part of sequels like "Raaz", "Jannat" and "Murder", actor Emraan Hashmi seems to have had enough of it and says he will be doing no more of it in the near future.
"I don't think I will be doing a sequel for a while after this. But these films have been successful from the beginning of my career, and nowadays a fashion of sequels is on, so we thought that it's great that we should take hit films ahead and make them into a series," he said in a group interaction.
"Raaz" franchise started in 2002 and its second instalment came in 2009 and it was titled "Raaz - The Mystery Continues".
Produced by Mahesh Bhatt and Mukesh Bhatt, "Raaz 3" revolves around the downfall in an actress's life and how she turns to black magic. It has been directed by Vikram Bhatt and also stars Bipasha Basu and Esha Gupta.
What makes Emraan is the commercial appreciation that his films get.
"I am happy and I hope that it stays that way. I always wanted to become a popular actor from the beginning of my career. I always wanted that my films have some sort of commercial aspect and draw mass audience," he said.
After being part of films like "Shanghai", the actor is on a lookout for something new.
"The character should be good and different and something which I have not played before. I also see the commercial viability of the film and how different it is... what kind of change it brings," he added.
Do you like this article?
|User Rating
|(0 Votes)
I'm glad to know that Emraan is taking a step from the 'trend of nowa days.' I've had appreciation for Emraans acting and choice of movies since I saw him first in Tumsa Nahin Dekha and then in Murder, Ashiq Banaya Apne... A great actor, hope so see him in more winning movies.
Delhi-based theatre artist Rachaittri Gupta will play the female lead ...
Emraan Hashmi's onscreen wife in his next is finalized! 1
Views : 874
Comments :0


text:
Coverking® Semi-Custom Class A RV Covers. The best in protection for your home away from home!
For your RV investment, Coverking® offers only the finest fabric available on the market - Presidium. Presidium fabric is heavy-duty, double-stitched 600D polyester, double poly-coated on both sides for the best in all-weather protection.
They even protect against man-made hazards like dents, dings, and acid rain. All Coverking RV Covers are designed with an easy-access zipper and quick-release buckles with nylon straps to hold it securely in place. Available in 6 sizes to fit most Class A RVs.
State Size, as available in the Shopping Cart.
More Details...


text:
Long before there were "hybrid cars" there were basic little internal combustion powered city cars, and for five-and-a-half decades, the UK's MINI was definitely on that list. Still posing as minimalist, since 2001 the brand has been a jewel in German automaker BMW's portfolio of "premium" products, and a more upscale lifestyle statement than from […]+ Read More
Toyota's Prius c has established itself solidly as the third-best-selling hybrid being the lowest price Prius, and the highest mpg car "without a plug" sold in America. The subcompact c is a full hybrid and one of four Prius "family" members, the others being the regular "Liftback," the wagon-like Prius v, and the Prius Plug-in […]+ Read More
An arguably corny news angle, Honda's communications department has been playing up the Halloween "trick or treat" line for the dealer launch of it's Accord full hybrid today. Fortunately for the Japanese automaker, it can be as cheesy as it wants because its engineers can back up the giddiness with something that looks like it […]+ Read More


text:
Suzannah Starkey: Why I've decided to become a fruit farmer
Posted: May 25, 2014|
In her first regular column for the Post, Suzannah Starkey reveals why she's taken the giant leap of becoming a fruit farmer at Starkey's Fruit in Southwell
IT seems as if I've been thrown in the deep end of a huge vat of apple juice – baptism by fruit.
A few months ago my father, a third generation farmer, asked me to steer a well-oiled ship onto the next stage, so that he could retire knowing that a good business and a wonderful heritage would be preserved. I could have said no, but I didn't actually want to. It seems absolutely the right thing to do.
Related content
'Town has 8,000 new jobs' claim as candidates clash
Nottinghamshire strawberries: Mediterranean temperatures brings bumper crop
Yasmin calls for more female role models in engineering
Palace where Charles I was held is stirred from slumbers
Warning as heavy rain forecast for Nottingham
Suzannah Starkey: Bees giving our blackberries a helping hand
Now scientists warn 'killer' strawberries are in the fridge!
Suzannah Starkey: Keep your wits about you on the farm
Suzannah Starkey: Discovering the Discovery apple
I have given up a party planning business in South East Wales to take on a role in a world I have always loved, but skirted around; although some knowledge must have seeped into my consciousness, as I have been involved in growing, producing and marketing all the time I was a wife, mother and budding businesswoman. That's normal for us girls!
I have been accompanying Dad on his rounds of the orchards and strawberry fields since I was seven.
With a Labrador trotting by our side, we would sniff the pinkening soft fruit, and admire the bulging apples – but during my childhood and teens, I never thought I should be concentrating on the details too much.
I wish I had listened and taken notes then, because now I have to learn a lot and fast. Dad used to write to me at boarding school with updates - I should dig those letters out!
The 40 years of strawberry growing have been a long and steep learning curve for the Starkeys, and Dad and his team have a huge knowledge that I can glean from.
There are 50 acres of soft fruit and top fruit growing here at Norwood, first planted as a diversification by my great grandfather, John Starkey, in 1901.
The archived accounts show a spidery yet neat handwriting, and daily diary notes about the weather. (Dad still takes a daily rain measurement.) Photographs in sepia show an Edwardian gentleman proudly standing by his new trees and, during WW2, ladies packing in wooden boxes with headscarves and grimaces.
I will be starting my apprenticeship by following Steve round the orchards and tunnels, listening and watching, and shadowing Trevor in the packhouse as he manages the wonderful team of pickers and packers. I will be learning about distribution and marketing and the science of growing fruit successfully. I will join every organization and association for extra guidance and support, and be championing lady farmers too, of course.
Mostly, I will be enjoying getting out of the high pressure kitchens and marquees of the private party business and being able to watch the seasons unfold up close and personal.
I look forward to taking you on my journey.


text:
I don't expect the government to take care of me, ever. I see bad things coming. I'm doing my best to not be overly dramatic, or bitter, but I don't see good things ahead. If/when it all goes to shit, and there is no food, I want to make sure we are covered, at least for a while. .
The irony here, is that I have a concealed carry permit and a hand gun that I keep in a safe. If Obama had lost, I would have started carrying it on me at all times. I knew they would make good on their threats to riot and murder.


text:
Badges may be purchased at the door for the listed walk-in prices.
A badge is required for admittance into all convention functions. Badges may be purchased online prior to -or onsite during- the convention, and are picked up at Registration. Children under the age of five do not need a badge. Everyone else does. This includes -but is not limited to- parents, guardians, and chaperones. There are no special prices, parent passes, parent discounts, et cetera available. Memberships grant access to every convention event except any special event requiring a separately purchased ticket. E.g., the formal ball requires a separate ticket.
Memberships are not be mailed. Pre-registered attendees will receive an email confirmation. Bring the confirmation email and a photo ID to pick up your badge from the pre-registration area of Registration. Badge pickup is available as early as the Thursday evening prior to the convention.
Attendees can only pick up their badge. Please bring -and have ready- a photo ID of yourself and your confirmation letter. The letter can be printed or displayed on a smartphone, or similar device. Minors without a photo ID must be accompanied by a parent or guardian in order to receive their badge. Minors need to present their confirmation email as well.
Email firstname.lastname@example.com if you have any questions or comments regarding Registration.


text:
Home Concert Tickets Clubs and Dance Justice
Justice Tickets
Get your Justice tickets on viagogo, your online ticket marketplace. Choose an event to see the selection of tickets available or check the calendar view for all the available dates with tickets. You can also sell your spare tickets on viagogo, and listing tickets is free. All Justice tickets are covered by the viagogo Ticket Guarantee!
Sign Up for Justice Ticket Alerts
Sign up here for information on upcoming onsale dates, times and venue information. Be the first to know when Justice tickets go on sale!
The email address you entered is invalid
Can't find the event you're looking for? Tell us about it!


text:
News & Public Affairs
Uhuru movement winds up Days in Solidarity national tour in St. Petersburg listen10/24/13 Janelle Irwin
WMNF Drive-Time News Thursday Listen to this entire show:
Tags: Uhuru, African American, African People, solidarity, social justice
Members of the Uhuru movement and their allies have been rallying in cities across the country in what they call their Days in Solidarity with African People. WMNF spoke with Stephanie Midler, the national chair of the Uhuru Solidarity movement about the conclusion of the tour Thursday night at the Uhuru House in Midtown St. Petersburg.
Be the first to comment
"The Days of Solidarity with African People is actually a nationwide campaign. The event tonight in St. Petersburg is the final event of a nationwide tour...
- 2015
- 2014
- 2013
- 2012
- 2011
- 2010
- 2009
- 2008
- 2007
- 2006


text:
2011 Kenworth T660
Gulfport, MS
2016 Freightliner BUSINESS CLASS M2 106
Somerville, NJ
2013 Chevrolet 3500HD
New Hampton, IA
2013 Dodge RAM 3500
Jacksonville, FL
2007 International 9400i
Lake Butler, FL
2009 Freightliner CST120
Des Moines , IA
Trailer for 2016 Dorsey 60 Ton Steel Drop Decks
Voorhees, NJ
2011 Freightliner CA12564DC - CASCADIA
Healdsburg, CA
2001 Western Star 4964FX
Ocala, FL
2010 International PROSTAR
Tampa, FL
Description: 1: NO CREDIT CK - NO TURN DOWNS - PAYMENTS U CAN AFFORD! ** LET US NEGOTIATE A PAYMENT FOR YOU **
3: Very Well Maintained Trucks.
5: DOT TIRES, Oil Change Every 8000 Miles, NO BLOW BY, NO OIL LEAKS
6: We have most service records, on most of our trucks Liners and Bottoms of Pistons
7: Runs and Drives Excellent Like New, FUEL SQUEEZER
8: P A Y M E N T S -U- C A N - A F F O R D Every-1-...More Information
Description: clean day cab, fleet truck, manual transmission, allum wheels, jakesMore Information
Description: Beautiful baby blue 2010 Volvo 430 Flattop sleeper.Low miles, Cummins ISX power, 10 sp manual trans!Excellent condition inside and out and nicely equipped.PM serviced, DOT ready and professionally detailed.90 Day Power-train warranty comes standard.Please call to reserve this great truck today! | LOW MILES - LIGHT WEIGHT - ISX POWER!More Information
Description: Beautiful baby blue 2010 Volvo 430 flattop sleeper.547,000 miles, Cummins ISX power, 10 sp manual trans!Excellent condition inside and out and nicely equipped.PM serviced, DOT ready and professionally detailed.90 Day Power-train warranty comes standard.Please call to reserve this great truck today!Very Light weight truck! | ISX POWER! LIGHT WEIGHT! VERY CLEAN!More Information
Description: NoneMore Information
Description: 9 SPEED,UNDER $60K! | AFFORDABLE & FLEET MAINTAINED!More Information
Description: Dyno Tested/Oil&filter changed/Dot passed. New Mattress/NewTriangle&fire Ext. Money Saving 90day/25,000 mile Engine, Transmission and Rear End limited warranty at no cost to you.Call for details. Also, ask about our Extended Warranty on this truck as well as our new 24/7 Roadside Assistance Program! | I-Shift, 25000 Mile Warranty!!!More Information
Description: GREAT TRUCK FOR 1ST TIME BUYERS! | HIGH PERFORMANCE,LOW PRICE!More Information
Description: This 630 is a from a well respected fleet and they are highly desirable! They have strong Cummins power moved by Fuller10-speed manual trans. Stand up mid roof sleeper with floorto ceiling storage. Volvo's "Smart Wheel" option to controlyour radio and lights while keep your hands on the wheel. Apair of air ride 8 way seats provide long lasting comfort. Dual power windows and mirror are convenient. | GREAT CONDITION INSIDE AND OUT!More Information
Description: Drive Tires 100% Virgin, Steers 75%, Brakes 50%More Information
Description: Raised RoofMore Information
Description: Nicely equipped beautiful red Volvo VNL 670 condo sleeper!VED 13 Volvo power w/ 485 horsepower!Fuller 10 speed manual transmission & 342 gear ratio.Aluminum wheels, Full skirts & great curb appeal.Excellent condition, PM serviced, DOT'd & detailed!90 Day confidence+ power-train warranty included.Please call for all the info on this great truck! | FLEET MAINTAINED NICELY EQUIPPED VNL670!More Information
Description: Beautiful Viper red 2010 Volvo VNL 670 w/ hard to find ISX!Cummins ISX engine w/ 10 speed manual trans & 336 ratio.Aluminum wheels, skirts and excellent rubber!Nicely equipped inside and out w/ many options!D.O.T ready, PM serviced & professionally detailed.90 Day confidence+ pwer-train warranty included.Please call today for all the info on this great truck! | ISX POWERED VOLVO VNL 670! 1 SWEET RIDE!More Information
Description: FRESH DOT & OIL CHANGE,READY FOR WORK! | DOT Ready!More Information
Description: UNDER $60K!YOU GET A LOT OF TRUCK FOR YOUR MONEY!FRESHDOT & OIL CHANGE! | Affordable!More Information
Description: AS IS ONLY | 13 SPD,BIG BUNK,D13 POWER,BUY IT NOWMore Information
Didn't find what you were looking for? Click here to request availability from our dealer network.


text:
Patient survival rates after liver transplants at Ochsner are higher than the national average.
Average wait time to a liver transplant at Ochsner is shorter than the national average.
Liver Transplant Rate for Wait List Registrations
Ochsner's Liver Transplant Program has continued to increase the annual volume of liver transplants performed.
Source: Scientific Registry of Transplant Recipients (SRTR)


text:
Students Return To Brookman Elementary School
(Las Vegas)–Students are back at Brookman Elementary School. They were evacuated due to malfunctioning air condition and "problems with water and electricity". CCSD officials confirm with KXNT the students were taken to nearby El Dorado […]
Study Says Home-Packed Lunches Less Nutritious Than School-Made Meals
A new study finds that what parents pack from home is often much worse than what's offered at school.
Pediatricians' Rx For Schools: Later Start Times
Pediatricians have a new prescription for schools: later start times for teens.
Buses Set To Roll
(Las Vegas, NV) — School buses are read.y to roll in Las Vegas.
School Starts Monday
Las Vegas– As a reminder, local police and the Nevada Highway Patrol want to remind drivers that most schools in Clark County resume on Monday. Authorities are asking drivers to "get back into the swing […]
Time-Saving Tips For Healthy School Lunches
With creative planning and the right containers, packing a quick and healthy lunch can be as easy as ABC and 123!
Back To School Study Tips For Teens
Help your kids make the most of their time when they head back to school with these 5 study tips.
5 Back To School Things Teachers Would Love Your Help With
Seasoned instructors share the essentials for your child's transition back to the classroom.
State: Test Scores Were Altered At Kelly
An investigation by the Nevada Department of Education has determined standardized test answer sheets at a Las Vegas elementary school were altered by adults and led to skyrocketing scores from one year to the next.
Kindergartner Brings BB Gun To Reno School
Washoe County school district officials are investigating after a kindergartner brought a BB gun to a Reno elementary school.


text:
This listing is no longer available. Please see other available listings.
Springtree Apartments - We have the home for you! - 253 582 4007
We have freshly prepped apartment homes waiting for you. Apartment grounds are fully fenced with video surveillance and we have full-time office and maintenance staff ready to assist you. Military discount available with 12 month lease. We actively participate in Lakewood's Crime-free Program.
Springtree Apartments are family owned and operated! Our on-site, full time maintenance and office staff are ready to serve you 24 hours a day. All parking is assigned permit only and carefully enforced so that your car always has a nice place to stay. You will be close to Lakewood, Lakewood Towne Center, McChord Field (only 4 blocks to main gate), Ft. Lewis, schools, restaurants, Tacoma Mall, and more!
Because Springtree is family owned and operated, we promise to:
* Give you great value for your hard
earned money.
* Be friendly and helpful to you.
* Listen to you, and always work to
make living better at Springtree
* Treat you fairly and with respect.
* Have staff that lives on site for your
peace of mind.
* Keep a watchful eye on the property.
* Provide prompt action on your
maintenance requests.
* Provide for repair of normal wear and
tear at no charge to you.
* Remain certified Crime-free by City of
* Provide full-time video recording of
common parking areas.
Call today at 253 582 4007 or visit http://www.springtree4you.com for details.
Map of 12713 Addison St SW, Apt #6, Lakewood, WA 98499
Contact Us
Springtree Apartments
(253) 582-4007


text:
Book your room at Pousada São Luís
Please enter your travel dates.
Get an overview of this hotelClothing optional pousada with free breakfast and rooftop terrace
Collect 200 points
Simply enter your Nectar card number when prompted. Your points will be credited 30 days after you return from your trip.*Offers may apply
Situated in Ouro Preto, this pousada is steps from Aleijadinho Museum and Our Lady of Conception Church. Chico Rei Mine and Sao Francisco de Assis Church are also within 10 minutes.
Property Features
A rooftop terrace, self parking, and a 24-hour front desk are available at this pousada. Free buffet breakfast and free WiFi in public areas are also provided. Other amenities include barbecue grills.
Room Amenities
All 17 rooms offer free WiFi, minibars, and LCD TVs with satellite channels. Hair dryers, showers, and phones are among the other amenities that guests will find.Information missing or incorrect? Tell us!
Hotel Amenities
Hotel Amenities
A complimentary breakfast is offered each morning. Public areas are equipped with complimentary high-speed wireless Internet access. This Ouro Preto pousada also offers a rooftop terrace and barbecue grills. Limited onsite parking is available on a first-come, first-served basis (surcharge).
- 24-hour front desk
- Total number of rooms - 17
- Barbecue grill(s)
- Free breakfast
- Free WiFi
- Parking (limited spaces)
- Rooftop terrace
- Self parking (surcharge)
Family Friendly Amenities
- Free Wi-Fi
- Rollaway/extra beds available
- Cribs/infant beds (complimentary)
Available in all rooms: Free WiFi
Available in some public areas: Free WiFi
Self parking (surcharge), Parking (limited spaces)
Room Amenities
- Air conditioning
- Phone
- Minibar
- Daily housekeeping
- Private bathroom
- Free toiletries
- Hair dryer
- Shower only
- Iron/ironing board (on request)
- Desk
- Rollaway/extra beds available
- Satellite TV service
- Free WiFi
- Free cribs/infant beds
Where to Eat
A complimentary buffet breakfast is served each morning between 7 AM and 10 AM.
Hotel Policies
Check-in time starts at 2 AM
Minimum check-in age is 18
Check-out time is noon
Payment types
You need to know
Extra-person charges may apply and vary depending on hotel policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
Only service dogs are allowed
- Rollaway bed fee: BRL 95 per night
We should mention
Parents traveling with a child under 18 must present the child's birth certificate and photo ID (passport for foreign visitors) upon check-in. If only one parent or legal guardian is traveling with the child, that parent or legal guardian must also present a notarized consent of travel signed by both parents. Visitors who plan to travel with children should consult with a Brazilian consulate office prior to travel for further guidance. Clothing is optional at this property.


text:
New Museum Relaunches Website
To celebrate the Museum's 35th Anniversary, the award-winning New Museum website (newmuseum.org) has been "reconceived as a unique destination with four major new programs designed to strengthen and serve our digital audience-now in the millions annually. In addition to programmatic expansion, the site will provide enhanced functionality, integration of several social media platforms, increased exhibition-related content, a dedicated section for Members, and mobile device optimization."
"The New Museum website is a new New Museum where we will experiment with innovative material for a rapidly growing online audience," said Lisa Phillips, Toby Devan Lewis Director. "We are proud that our new site not only reflects the dynamic, global scope of the Museum's programs but also expands resources for visitors and scholars alike and introduces new initiatives at the vanguard of online engagement."
The culmination of four years of work, the redesign of the website and its four signature initiatives have been supported by extraordinary funding, totaling over $1 million, from the Andrew W. Mellon Foundation, Bloomberg Philanthropies, Booth Ferris Foundation, Henry Luce Foundation, and the Institute of Museum and Library Services received over the past five years. The grants supported back and front end rebuilding, upgrades to our IT infrastructure, and new staff positions related to these initiatives. Additional Museum staff and resources have been redistributed to meet the growing needs of our online audience.
The re-envisioned website premieres four major new online initiatives to:
- expand the conversation about contemporary art in a global context
- premiere new works
- share our history
- provide expanded resources on exhibitions and programs both past and present
About Author
- Casting Roundup: Sarah Chalke, Kim Raver Headline New Pilots
- ADVENTURE TIME Headed to the Big Screen?
- Pilot Season Roundup: Colm Feore, Daniel Stern, Dave Annable to Star in New Shows
- James Franco to Star in New Fox Comedy, WHY HIM?
- Christopher Meloni to Star in WGN America's Slave Drama UNDERGROUND
- Check Out Alex Dunphy's Full College Essay from Latest MODERN FAMILY Episode
- Photo Flash: HBO Reveals Key Art for GAME OF THRONES Season 5
- Syfy to Honor Leonard Nimoy with Special Programming This Sunday
- Grammy Winner Kelly Clarkson Exclusively Premieres New Music from New Album on MTV, VH1 & CMT First
- CONSTELLATIONS' Jake Gyllenhaal and Ruth Wilson Set for THEATER TALK This Weekend


text:
I am not sure if this was already here in 4.0.4 (it wasn't in 4.0.1), but I am glad to see that it is possible to set phone lock timeout again. Back when I used HTC Desire I had a PIN code on my phone for casual security, but since at times I switched the screen off and soon after on again (like when in a store to look at something) I didn't want to enter the PIN all the time. So I had a time lock set for it, that it only asked for PIN if I didn't use the phone for five minutes.
This was not an option in Android 4.0. If you used PIN security then every time you turned off the screen and back on again, it asked for a PIN. Especially horrible when it timed out. It became an annoyance enough that I ended up using Slide Lock only.
Now it's possible again to set the timeout forphone lock, making sure that your phone is locked only after X amount of time. I love it! It even has an option to check if power button instantly locks or not.
|Thread Tools
|Search this Thread
|Display Modes
Linear Mode


text:
The CWU Women's Rugby Club is back in action Saturday, Nov. 3 as they square off against the University of Washington at 11 a.m. on the Nicholson Rugby Field. Admission is free and open to the public.
Come support the club as they attempt to send the Huskies back to Seattle with a loss. If Central wants to win this match, it will need to outmatch the Huskies physically and take advantage of scoring opportunities.
For more information call 509-963-3516 or email email@example.com.
When Megan Schmitt submitted entries in the 8th annual Expression Awards competition for digital sigThe CWU Publicity Center's Student Employees Shine
CWU provides its students with the opportunity to not only earn degrees, but to achieve excellence iPublicity Center Designers Win Local, Regional ADDY Awards
Central Washington University's Publicity Center received three ADDY awards from the local chapter


text:
Now that spring is here, ReeferTek is entering the busiest and best time of year for manufacturing refrigerated vans. If you are considering buying or leasing a refrigerated van, now is a great time to take advantage of our spring promotions.
Any business that depends on refrigerated transportation knows the importance of having a reliable refrigerated van. Whether you deliver frozen foods, flowers, dairy products, or produce, you shouldn't have to worry about the performance of your refrigerated van. Your business depends on it. With this in mind, wouldn't you want to partner with the most established refrigerated van manufacturer in the industry?
If you're considering buying a new refrigerated van, do some research ahead of time. You want ensure you are comparing "apples to apples" with features. For example, understand what type of material is used to insulate the van. ReeferTek USA uses the highest density closed-cell polyurethane foam for maximum R-value insulation. Many of our competitors are using inferior insulation products including fiberglass. Proper insulation is the most important factor in manufacturing a refrigerated van. This is how goods are stored and transported at the precise temperature.
For more information on refrigerated vans or to customize your own vehicle, call ReeferTek today at: 855-534-2626


text:
Press release from PR Newswire
Cameron Third Quarter Earnings Release Conference Call
Tuesday, September 11, 2012
Cameron Third Quarter Earnings Release Conference Call14:27 EDT Tuesday, September 11, 2012HOUSTON, Sept. 11, 2012 /PRNewswire/ -- In conjunction with Cameron's (NYSE: CAM) Third Quarter earnings release, you are invited to listen to its conference call that will be broadcast live over the Internet on October 31, 2012 at 9:30 a.m. Eastern. What: Cameron Third Quarter Earnings Release Conference Call When: October 31, 2012 at 9:30 a.m. Eastern Where: http://www.c-a-m.com How: Live over the Internet - Simply log on the web address above. Contact: Jeff Altamari, Vice President, Investor Relations(713-513-3344)Or email@example.com may also listen to the call by dialing 201-689-8261 at least 10 minutes prior to the start time. The replay may be accessed by calling 201-612-7415 (Conference Call #400352).Cameron is a leading provider of flow equipment products, systems and services to worldwide oil, gas and process industries.SOURCE Cameron


text:
A method is disclosed for the discrimination between intact and damaged cells in a sample, and more particularly, the method uses a vital nucleic acid dye to selectively stain intact versus damaged cells which then may be counted and sorted by flow cytometry means. The method also may be used in conjunction with fluorescently labelled monoclonal antibodies to simultaneously identify cellular antigens.
1. A method for discriminating between a heterogeneous population of intact and damaged cells in a peripheral blood sample comprising the steps of:
a) taking a sample of peripheral blood from an individual;
b) adding to the sample one or more monoclonal antibodies to cell surface antigens, wherein the monoclonal antibodies are labeled with fluorescent labels and wherein the fluorescent labels have peak emission spectra that are distinguishable from each other and from a nucleic acid dye;
c) adding a fixative to the sample;
d) adding to the sample a nucleic acid dye which preferentially binds DNA, wherein the nucleic acid dye comprises the formula ##STR2## wherein n is 0;
m is 1;
R is a lower alkyl;
R1 and R2 are lower alkyls;
R3 is hydrogen;
Z is a quinoline nucleus which may be substituted with lower alkyl, halo, nitro, amino and dialkylamino; and
X represents an anion;
e) analyzing the sample in an automated instrument capable of passing the cells in the sample through a sensing region substantially one at a time and capable of detecting and recording both fluorescence and light scattered in more than one direction by each cell passing through said sensing region; and
f) discriminating between damaged and intact cells in the sample based upon relative fluorescence intensity of the nucleic acid dye in each cell and light scattered.
2. The method of claim 1 wherein erythrocytes in the sample are lysed.
3. The method of claim 1 wherein the automated instrument is a flow cytometer.
4. The method of claim 1 wherein the fixative is paraformaldehyde.
5. The method of claim 1 wherein the fluorescent label is fluorescein isothiocyanate.
6. The method of claim 1 wherein the fluorescent label is phycoerythrin.
7. The method of claim 1 wherein the fluorescent labels are fluorescein isothiocyanate and phycoerythrin.
8. The method of claim 1 wherein the intact and damaged cells are further discriminated based upon relative fluorescence intensity of fluorescent labels and light scattered.
9. A method for discriminating between a heterogeneous population of intact and damaged cells in a peripheral blood sample comprising the steps of:
a) taking a sample of peripheral blood from an individual;
b) lysing erythrocytes in the sample;
c) adding to the sample one or more monoclonal antibodies to cell surface antigens, wherein the monoclonal antibodies are labeled with fluorescent labels and wherein the fluorescent labels have peak emission spectra that are distinguishable from each other and from a nucleic acid dye;
d) adding paraformaldehyde to the sample;
e) adding to the sample a nucleic acid dye which preferentially binds DNA, wherein the nucleic acid dye comprises the formula ##STR3## wherein n is 0;
m is 1;
R is a lower alkyl;
R1 and R2 are lower alkyls;
R3 is hydrogen;
Z is a quinoline nucleus which may be substituted with lower alkyl, halo, nitro, amino and dialkylamino; and
X represents an anion;
e) analyzing said sample in a flow cytometer equipped with a single laser tuned to 488 nm; and
f) discriminating between damaged and intact cells in the sample based upon relative fluorescence intensity of the nucleic acid dye in each cell and light scattered.
10. The method of claim 9 wherein the intact and damaged cells are further discriminated based upon relative fluorescence intensity of fluorescent labels and light scattered.
This invention relates to a method for discriminating between intact and damaged cells in a sample, and more particularly, relates to a method for discriminating between intact and damaged cells in a peripheral blood sample, wherein the intact and damaged cells are discriminately stained by a nucleic acid stain.
The detection and identification of cell types in the hematopoietic system has long been a useful research and clinical tool. A number of automated methods exist to aid the researcher and clinician. Among those methods include flow cytometry and fluorescence microscopy. In recent years, the former method has become increasingly sophisticated and has become generally accepted as a tool to aid in the identification of or discrimination between cell types and between various functional and/or maturational subsets within a cell type.
Flow cytometers, which are more generally described in U.S. Pat. Nos. 4,661,913, 4,284,412 and 3,826,364, and in an article by Herzenberg et al., Sci. Am., 234:108 (1976), have been used to identify different populations of leukocytes in a heterogeneous sample by detecting multiple independent parameters on individual cells that pass through the sensing region. Typically, these parameters include forward light scatter (FLS, which is a measure of relative particle size), orthogonal light scatter (OLS, which is a measure of relative granularity) and fluorescence. Fluorescence may be measured from cells that incorporate a nucleic acid or other vital stain or may be measured from cells bearing surface markers which are labelled with monoclonal antibodies (MAbs) that are conjugated directly or indirectly to fluorochromes as described, for example, in U.S. Pat. No. 4,520,110. By combining and comparing these parameters, the various leukocyte components may be distinguished.
U.S. Pat. No. 4,727,020 provides one example of how a flow cytometer operates and may be used to identify leukocyte subpopulations. Unlysed whole blood was treated with one MAb conjugated to phycoerythrin (PE) specific for CD4+ T cells and and a second MAb conjugated to fluoroscein isothiocyanate (FITC) specific for CD8+ T cells. A nucleic acid dye, LDS-751™ (Exciton), was added to identify nucleated leukocytes. The labelled cells then were analyzed by flow cytometry. A gate was set for LDS-751+ cells (i.e., for nucleated leukocytes, thereby excluding erythrocytes and platelets). The method allowed separation of leukocyte subpopulations by comparing the various parameters measured.
One problem inherent in any method that makes use of fluorescently labelled MAbs and/or nucleic acid dyes, however, is the propensity of the labels to indiscriminantly bind to damaged cells or cell debris in a sample. This problem is compounded by the fact that in order to prepare cells for labelling, the cells must undergo several preparation techniques, all of which increase the number and proportion of cells that become damaged or ruptured in any one sample. These damaged cells and associated cell debris and their accompanying fluorescence must be discriminated against in the overall sample in order to thoroughly examine the remaining intact cells. Depending upon the method used to prepare the cells in any one sample and even between samples using the same method, sample preparation can introduce a significant amount of variation into the system. As a result, method used for immunofluorescence analysis of cells could mis-identify damaged cells as part of the subpopulations of interest.
A variety of techniques exist for determining whether a cell in a sample is intact or damaged. Viable, intact cells can be distinguished from dead cells by using either fluoroscein diacetate (FDA) or propidium iodide (PI). In these methods, the sample is treated with either FDA or PI and then examined for fluorescence. Cells that stain with FDA are considered "viable"; cells that stain with PI are considered "dead." The methods are limited, however, in that the cells cannot be fixed if the stains are to be used to identify viable cells. Another limitation on the use of FDA is that it brightly fluoresces so that it overwhelms the immunofluorescence signals from other stains, such as FITC and PE, rendering them unreadable.
Other methods to detect intact cells that do not make use of such dyes also exist. FLS, on a flow cytometer for example, may be used to discriminate between intact and damaged cells but only if the cells in the sample are derived from a homogeneous population. Cells from a heterogeneous population cannot be so distinguished because of variations in cell size and light scattering properties.
Each of the above-described methods suffers from some defect that makes it inapplicable as a general method for discrimination between damaged and intact cells in a heterogeneous sample. As a result, there is no single method that allows the researcher or clinician to examine a heterogeneous cell sample from an individual and to discriminate between damaged and intact cells therein.
The present invention comprises a method to discriminate between intact and damaged cells in a body fluid sample. The method comprises the steps of: 1) taking a body fluid sample from an individual; 2) adding a nucleic acid dye to said sample; and 3) analyzing the cells in said sample in an automated instrument capable of passing said cells through a sensing region substantially one at a time and capable of detecting and recording both fluorescence and light scattered by individual cells.
In the preferred embodiment, the body fluid sample comprises peripheral whole blood wherein the erythrocytes are lysed, and the nucleic acid dye comprises a stain with a preference for DNA and which also distinguishes between damaged and intact cells based on the amount of fluorescence emitted. It will be appreciated by those skilled in the art that this method also is particularly useful when combined with other methods used to identify cell types (e.g., when fluorescently labelled MAbs are used). Examples of such other methods include those described in U.S. Pat. No. 4,599,304 for the monitoring of activated cells. In such cases, it will be further appreciated that the peak emission spectra of each of the fluorochromes used to label the MAbs and of the nucleic acid dye must be sufficiently distinct so as not to overlap. Similarly, it is desirable that all of the fluorescent labels be excitable at the same wavelength. This will allow the use of a single laser source in the flow cytometer, as opposed to having a dual laser source as described in U.S. Pat. No. 4,727,020.
A kit comprising a set of containers separately containing a nucleic acid dye and one or MAbs also is disclosed.
FIGS. 1A-1E comprise several correlations of "dot" plots of OLS vs. FLS (A,C-E) and log LDS-751 fluorescence vs. FLS (B) for fixed PBL (peripheral blood leukocytes) from erythrocyte depleted whole blood;
FIGS. 2A-2E comprise several dot plots of OLS vs. FLS (A,D), log LDS-751 fluorescence vs. FLS (B) and vs. log FDA fluorescence (C,E) the PBL were not fixed;
FIGS. 3A-3E comprise several dot plots as in FIG. 2 for cells prepared from the same individual as in FIG. 2, but the cells were held for 48 hours prior to staining with LDS-751 and FDA;
FIGS. 4A-4E comprise several dot plots of log CD5(PE) fluorescence vs. CD20(FITC) fluorescence (A,C,E), log LDS-751 fluorescence vs. FLS (B) and FLS vs. OLS (D) for erythrocyte depleted whole blood which had been reacted with these two MAbs;
FIGS. 5A-5E comprise several dot plots as in FIG. 1 but wherein the cells also were stained with CD11b(PE) and CD15(FITC); and
FIGS. 6A-6E comprise several dot plots of log CD11b(PE) fluorescence vs. log CD15(FITC) fluorescence (A,C-E) and log LDS-751 fluorescence vs. FLS (B) for the fixed PBL from erythrocyte depleted whole blood as shown in FIG. 5.
The present invention comprises a method for the discrimination between and identification of cell types in a body fluid. Preferably, the body fluid is erythrocyte depleted peripheral blood; however, other body fluids that may be sampled include peritoneal, spinal and brain fluids, as well as urine. In addition, cell suspensions of bone marrow, lymph node, liver, spleen and thymus may be used.
The method comprises the steps of:
1) taking a body fluid sample from an individual;
2) adding a fluorescent nucleic acid dye to said sample; and
3) analyzing the cells in said sample in an automated instrument capable of passing said cells through a sensing region substantially one at a time and capable of detecting and recording both fluorescence and light scattered by each of the cells that pass through the sensing region.
The method may be broadened to include a step wherein MAbs are added to the sample prior to staining with the nucleic acid dye. One or more MAbs may be used to detect cell surface antigens or the cells in the body fluid sample. The method is not limited by the MAb used or the cell surface antigen to be detected; however, certain pairs of MAbs are useful in the practice of this invention and include those pairs described in U.S. Ser. No. 126,333, filed Nov. 30, 1987 (commonly assigned to Becton, Dickinson and Company). The MAbs may be directly or indirectly labelled by one or more fluorochromes by methods known to those skilled in the art. It is preferable that the MAbs are directly labelled. It also is preferable that the peak emission spectra of the fluorochromes and the nucleic acid dye all be distinguishable. Further, it is desirable, when a single laser source is used in the automated instrument, to have all the fluorescent materials excitable at substantially the same wavelength (e.g., 488 nm when an argon laser is used). If a dual laser source is used, the excitation spectra may differ.
Desirably, the nucleic acid dye has a preference for DNA which has different fluorescence intensities for intact and damaged cells. In this manner, non-nucleated cells, such as erythroyctes and platelets, will not be stained (or will be stained minimally) such that nucleated cells (e.g., leukocytes) can be distinguished from non-nucleated cells based upon the intensity of fluorescence. In the preferred embodiment, the dye is LDS-751; however, other dyes having a preference for DNA also may be used such as those disclosed in U.S. Pat. No. 4,544,546. Representative nucleic acid dyes include dyes of the following formula ##STR1## wherein
n represents an integer having a value of 0 or 1;
m represents an integer having a value of 1 or 2;
R represents a member selected from the group consisting of lower alkyl, lower hydroxyalkyl, dialkylaminoalkyl, pyrrolidinoalkyl and morpholinoalkyl;
R1 and R2 each represent members selected from the group consisting of hydrogen, lower alkyl and lower haloalkyl;
R3 represents a member selected from the group consisting of hydrogen, lower alkoxy, lower alkyl and amino;
Z represents nonmetallic atoms necessary to complete a heterocyclic nucleus selected from the group consisting of a benzothiazole nucleus, indolenino nucleus, napthothiazole nucleus, benzoselenazole nucleus, benzoxazole nucleus, quinoline nucleus, and pyridine nucleus any of which may be substituted with lower alkyl, halo, nitro, amino and dialkylamino; and X represents an anion. The amount of dye used and the time the cells are stained with the dye should be sufficient to avoid differential staining as a result of kinetic phenomena. Twenty-four hours is more than sufficient time.
A variety of devices may meet the requirements of the automated instrument. Desirably, the instrument comprises a flow cytometer with a single laser source, Preferably, the single laser source is an argon laser tuned to 488 nm. The flow cytometer may further comprise data storage and analyses means such as a personal computer with software sufficient to list, store and analyze at least five parameters of data in various paired combinations. Examples of flow cytometers useful in the practice of this invention include FACS 440™, FACScan™ and FACStar™ (all commercially available from Becton Dickinson Immunocytometry Systems (BDIS)). Examples of personal computers and appropriate software include Consort 30™ data management systems (BDIS) and FACScan RESEARCH or PAINT-A-GATE™ software (BDIS).
It will be appreciated by those skilled in the art that a fluorescence microscope may be substituted for the automated instrument. In this case, manual counting and identification of cells is required.
Peripheral blood of healthy individuals was collected by venipuncture into VACUTAINER™ blood collection tubes containing ethylenediaminetetracetate as anticoagulant (Becton Dickenson). Erythrocytes were lysed with NH4 Cl. One volume of blood was mixed with 15 volumes of 1% NH4 Cl in H2 O(pH 7.3) and gently mixed. Cells were lysed for 3-5 minutes, and centrifuged at 200 g for 5 minutes at room temperature. The pellet was resuspended in a volume of a human cell culture medium which is available commercially as RPMI 1640™ (Whittaker) 14 times larger than the original blood volume and centrifuged at 200 g for 5 minutes. This washing step was repeated twice and the cells were finally resuspended in phosphate buffered saline containing 1% bovine serum albumin, 104 IE penicillin/ml, 100 u/ml streptomycin and 20 mM Hepes (pH 7.3) (PBS). The cell concentration of the remaining peripheral blood leukocytes (PBL) was adjusted to 1�107 /ml.
Where MAbs were added to the sample, twenty ul of a pretitered MAb was added to 100 ul of cell suspension. After incubating for 20 minutes on ice, the cells were washed twice with 2 ml of the PBS solution of 4� C. The staining procedure was repeated for a second MAb, if any. The pellet of the immunofluorescent labelled cells was resuspended in 1 ml of 1% paraformaldehyde in PBS.
A stock solution of LDS-751 was made by dissolving 0.2 mg in 1 ml of methanol. A working solution containing 0.5 ml of the stock solution was diluted into a final volume of 50 ml PBS containing 0.1% azide. Ten ul of the LDS-751 working solution was added to the paraformaldehyde fixed cells and kept overnight before flow cytometric analysis. The cell suspension could be kept at least two weeks without changes in the light scattering properties or fading of the immunofluorescence signals on the cells.
In the experiments correlating the fluorescence signals obtained from LDS-751 and FDA, the NH4 Cl lysed cells were resuspended in RPMI 1640 to a final concentration of 1�106 /ml Before analysis, the cells were kept one hour in the RPMI solution in order to obtain optimal light scattering properties of the cells. The FDA stock solution was made by dissolving 5 mg FDA in 1 ml of acetone. The FDA working solution contained 5 ul of the stock solution diluted into a final volume of 5 ml PBS containing 0.1% azide. Fifteen minutes before analysis, 30 ul of the FDA working solution was added to 1 ml of the cell suspension together with LDS-751 in a final concentrations of 0.2 ug/ml for the fresh and 0.1 ug/ml for the 48 hours old samples respectively.
The MAbs FITC labelled Leu-Ml. (BDIS) and PE labelled CD11b (Anti-Leu-15, BDIS) were used. With this combination NK-cells and different maturational stages of monocytes and neutrophilic granulocytes could be identified. The MAbs FITC labelled CD20 (Anti-Leu-16, BDIS) and PE labelled CD5 (Anti-Leu-1, BDIS) also were used.
Flow cytometric analyzes were performed on a FACScan™ flow cytometer. This instrument used an air-cooled Argon ion laser as a light source. The laser operated at 488 nm with an intensity of 15 mW. The laser was focused on the cell stream by means of a prismatic expander and a planoconvex lens providing a 20 um�64 um elliptical beam. The optical measurements were performed in a quartz flowcell with a 430 um�180 um rectangular flowchannel. A stable flow was achieved by pressurizing sheet and sample flow. Measurements were performed at a sample flow rate of 60 ul/min.
The light scattered in forward direction was collected with a spherical lens provided with a rectangular beam stop (collecting angles 1-10 degrees) and detected by a solid state silicon detector. Orthogonal scattered and fluorescence light was collected by a lens (H 94, 1.22 NA) coupled to the flowcell with an optical gel (collecting angles between 23� and 157�). The light was directed to 4 photomultiplier tubes using appropriate optical filter combinations. The fluorescence signals were collected through a 530 nm band pass filter for the FITC signals, a 585 nm band pass filter for the PE signals and a 650 nm longpass filter for the fluorescent light obtained from LDS-751. The scattered light was directed to a photomultiplier using a Brewster angle beam splitter.
The five parameters were digitized and stored in memory by Direct Memory Access in the list mode with Consort 30™. Each measurement contained 22,000 cells. Data acquisition was performed with the FACScan Research Software.
Cell sorting was performed on a FACStar™ flow cytometer. For each population 10,000 cells were sorted into RPMI containing 10% Fetal Calf Serum (FCS). The sorted cells were spun down for 5 minutes at 200 g and resuspended in 100 ul RPMI 1640 containing 10% FCS. Cytospin preparations were made with a Shandon Cyto-centrifuge (Southern Product Ltd., Astmoor, England). The slides were stained with Wright Stain and examined with a light microscope.
PBL were obtained by lysing the erythrocytes with NH4 CL as described. These cells were labelled with PE and FITC labeled MAbs following standard immunofluorescence procedures. After washing, the cells were fixed in 1% paraformaldehyde and the nucleic acid dye, LDS-751, then was added to the cells.
The instrument threshold was set at a low level on the forward angle light scattering channel in order to include platelets and cell debris in the analysis. See FIG. 1A. The major leukocyte cell populations including lymphocytes, monocytes, granulocytes, and the eosinophils (L,M,N,EO respectively, FIG. 1A) could be distinguished from the platelets and cell debris by forward and orthogonal light scattering signals.
In the correlation between the forward light scattering and LDS-751 fluorescence, FIG. 1B, a population of intermediate staining cells with significant light scattering was identified. See Population I, FIG. 1B. This population included all of the intact cells as identified by forward and right angle light scattering. See FIG. 1D. The population with the highest amount of LDS-751 fluorescence, DA in FIG. 1B, had low to intermediate amounts of forward and orthogonal light scattering. See FIG. 1C. The proportion of events within Population DA varied from sample to sample and differed with changes in preparation procedures. The low light scattering signals and the sample to sample variations suggested that these events were cell debris. This was confirmed by sorting the cell populations identified in FIG. 1B. The cells in Population I were intact cells by microscopic examination. The cells represented within DA were damaged cells, bare nuclei, or aggregated platelets. The particles identified within Population P were platelets and erythrocytes. This latter population had light scattering characteristics shown in FIG. 1E.
A comparison of FIG. 1C and D would suggest that the use of light scattering alone would include some damage cells with the intact cell populations. The contribution of damaged cells to the populations identified solely by light scattering was determined for twenty normal donors (Table I).
TABLE I______________________________________Enumeration of Percentage of Intact Nucleated Cellsof 20 Donors Determined by LDS-751 Fluorescencea MIN MAX MEAN S.D.______________________________________Intact Nucleated Cellsb 13 66 34 14.6Light Scattering gate forcIntact Lymphocytes 78 99 88 7.1Intact Monocytes 8 88 56 21.4Intact Neutrophils 47 91 76 11.4Intact Eosinophils 11 78 41 16.7______________________________________ a PBL obtained by NH4 C1 lysing, fixed in 1% paraformaldehyde and stained with LDS751. b Cells occurring the region indicated by "I" in FIG. 1B (% is obtained by dividing their number by the total number of nucleated cells (i.e., I + DA) � 100%). c Number of intact nucleated cells occurring in the light scattering gates typical for cell types as indicated in FIG. 1D divided by the numbe of cells occurring in identical gates set in FIG. 1A � 100%. To determine the percentage of cells which might interfere with the immunofluorescence, the percent of intact cells was determined in the light scattering regions typical for lymphocytes, monocytes, neutrophil granulocytes, and eosinophilic granulocytes (FIG. 1D: L,M,N,EO respectively). This table indicates that only a minority of cells (mean 34% intact nucleated cells) survived the sample preparation protocol. In addition, a significant fraction of these damaged cells could not be removed based on light scattering characteristics only. For example, within the lymphocyte light scattering gate (FIG. 1D: L) an average of only 88% of the cells were intact based on LDS-751 fluorescence. It should be noted that erythrocytes which survived the lysis protocol could appear in the light scattering region typical for nucleated cells. (FIG. 1E). By using the additional fluorescence parameter the cells were confined to Population P, FIG. 1B since they did not stain with LDS-751 and therefore could be distinguished from nucleated cells.
The extension of this technique to unfixed, viable samples added further confirmation that the LDS-751 was able to discriminate intact from damaged cells. LDS-751 can be used to stain viable leukocytes. This permits the correlation of LDS-751 staining with conventional dyes which assess viability in unfixed samples.
PBL were prepared using NH4 Cl without subsequent fixation. The light scattering characteristics of this sample are shown in FIG. 2A. The cells that are identified as viable using FDA can also be distinguished based on LDS-751 fluorescence. See FIG. 2C. Greater than 98% of the cells were identified as positive by both FDA and LDS-751 fluorescence.
In a similar experiment, the unfixed cells were held for 48 hours after the NH4 Cl lysis in order to increase the dead cells in the sample before staining with LDS-751 and FDA. FIG. 3. At this time, only 71% of the cells were viable based on FDA and LDS-751 fluorescence. By gating on the LDS-751 and light scattering, FIG. 3B, essentially all of the viable cells are included (98.5%) and only a few (8.6%) were dead based on FDA fluorescence. These data confirm that LDS-751 discriminates intact from damaged cells both in a viable state and after fixation.
The excitation of LDS-751 at 488 nm and its far red emission permits the combination of this dye with both FITC and PE immunofluorescence reagents. Spectral compensation must be used between the dyes to correct for PE emission entering the LDS channel (10% subtraction), however, only a minimal correction must be made in the other direction (2% subtraction). No compensation was required between the FITC and LDS-751 channels.
The advantage of using LDS-751 to identify damaged cells during lymphocyte subpopulation analysis is demonstrated in FIG. 4. In this example the erythrocyte lysed, whole blood was reacted with CD5(PE) (T-cells and a subpopulation of B-cells) and with CD20(FITC) (B cells). The intact cells were identified in the correlation between LDS-751 and forward light scatter. See FIG. 4B. The relatively infrequent population of CD5+ /CD20-, CD5+ /CD20+ and CD5- /CD20+ lymphocytes, FIG. 4E, can be identified by further gating on the forward and orthogonal light scattering. See FIG. 4D. By gating only on light scattering without identifying intact cells, the proportion of double labelled CD5+, CD20+ cells was increased by 50%. See FIG. 4C.
A second example illustrating the importance of combining immunofluorescence with the identification of intact cells is shown in FIG. 5 and 6. The cells in this example were labelled with CD15(FITC) and CD11b(PE). These different cell types labelled (i.e., monocytes, granulocytes and NK cells) can be distinguished based on quantitative differences in antibody labelling and on the differences in their light scattering characteristics. The CD15 MAb is known to agglutinate neutrophils since it is of the IgM isotype and the number of antigen sites on the target cells is high. This agglutination can be observed by comparing the relative frequency of the neutrophils, FIG. 5A and FIG. 1A, since these samples were obtained from the same cell preparation. It is evident that fewer neutrophils were observed by light scattering in the sample which was reacted with the CD15 MAb. It could be shown that the cells are still within the sample. As shown before, the intact cells, FIG. 5B (Population I), could be distinguished from the more brightly labelled larger cells identified in Population AG. These agglutinated cells comprising 20% the intact cell population had light scattering characteristics which were off scale in FIG. 5C.
The immunofluorescence of these cell populations is shown in FIG. 6. The agglutinated cells have the highest binding of both CD15 and CD11b. See FIG. 6C. These data indicate that the intensity of staining LDS-751 could also be used to identify doublets or agglutinated cells within the sample.
The discrimination between cell population based on CD15 and CD11b binding was more easily observed when the damage cells and doublets were removed from the sample by getting the list mode file. The multiple populations identified by immunofluorescence were also recognizable by light scattering. In comparing FIG. 5D and 6D, the population which are mapped by the immunofluorescences have corresponding light scattering characteristics (L,M,N,EO). The neutrophils (N) and eosinophils (EO) were separated by both forward angle light scattering and by the intensity of CD15 fluorescence. The monocytes (M) were distinguished from the lymphocytes (L) both by their light scattering properties and CD11b expression. The NK cells (included in the L Population) expressed CD11b but did not express CD15 as did the monocytes (M). The identity of these cell populations has been confirmed by sorting the cells for morphological analysis.
A kit comprising containers separately containing a nucleic acid dye and one or more MAbs may be used in the practice of this invention. In the preferred embodiment, one container in the kit will contain LDS-751. Other containers in the kit may include separately any of the MAbs previously described in the above-examples. It will be appreciated by those skilled in the art that the MAbs may be labelled before inclusion in the kit or separate containers containing the fluorochromes may be included for independent labelling.
All publications and patent applications mentioned in this specification are indicative of the level of skill of those skilled in the art to which this invention pertains. All publications and patent applications are herein incorporated by reference to the same extent as if each individual publication or patent application was specifically and individually indicated to be incorporated by reference.
It will be apparent to one of ordinary skill in the art that many changes and modifications can be made in the invention without departing from the spirit or scope of the appended claims.
Patent Citations
Non-Patent Citations
Referenced by
Legal Events
Original Image


text:
10 years ago – July 2004 U.S. Speaker of the House Dennis Hastert pays a visit to Forest Lake Elementary School to speak on behalf of Republican Sixth District Congressman Mark Kennedy…Dairy farmers feel relief as milk prices reach a record high of just over $20 per hundred weight, which is ...Read More
Yesteryears column
10 years ago – June 2004 Wyoming residents held a public demonstration in Chisago City's Moberg Park to protest Chisago City's plans to annex 5,000 acres of the township … A St. Paul man escaped injury after crashing his Cessna 150 into Clear Lake. The plane flipped upon impact in five f...Read More
10 years ago – May 2004 Family Pathways food pantry opens in Schilling Plaza, making itself available to an estimated 250 families and 1,000 individuals…Gas-powered scooters were banned from the city-owned bike paths and trails by the Wyoming City Council…Forest Lake residents Judg...Read More
10 years ago – April 2004 An update on the downtown Park Place building led the 2004 Progress Edition. The three-story commercial and residential condominium is filling up with construction complete…Faith Lutheran Church is planning a first-time event: a father-daughter dance…Forest La...Read More
10 years ago-March 2004 Tom Hefty earned write-in votes to defeat an incumbent in the race for Columbus Town Board. Incumbents Nancy Madden and Blair Joselyn retained seats in New Scandia, while Ron Swenson and Jeffrey Eiffler earned seats on the board in Wyoming. Melvin Pfaffendorf, Dave Tryon kept...Read More
10 years ago – February 2004 Bob Cockburn retired from the Forest Lake Police Department. He is the last of the force's original six members to retire. Officer Rick Peterson will take over investigative duties…Wyoming's Kathie and Jess Eliason were elected the Valentine's royal cou...Read More
10 years ago – January 2004 Jill Teetzel, East Bethel, is the new city administrator in Wyoming…Longtime Washington County judge David Doyscher of Forest Lake will soon leave for Kosovo, where he will work as a judge for the United Nations…Forest Lake High School graduate Courtland Nel...Read More


text:
Offering a romantic glow to your bedroom, the Arc Tempo floor lamp with the Trellis Hearts giclee shade is a wonderful addition to your sophisticated décor.
The great look of this floor lamp is matched by its practical design. The custom-made shade is attached by a rotating arm that enables you to swivel the shade in a full circle. Simple to adjust, this floor lamp can do double-duty as either a reading or accent light. The shade is printed on high-quality canvas with the giclee technique, allowing for the faithful reproduction of color and detail. Features an on/off foot switch. U.S. Patent # 7,347,593.
Our associates are standing by to assist you.


text:
Log in using your Facebook, Windows Live, Google or Yahoo! account
Or log in with your UEFA.com account
E-MailThere seems to be a problem with the email you have entered. Please check your address is valid and re-enter.
PasswordPlease choose a password
Keep me signed in
LoginForgot your password?
Despite scoring to help his side draw 1-1 away at FC Zenit, FC Porto captain Lucho González was left disappointed by the final result in Russia, with Andrey Arshavin admitting to UEFA.com that his team were "lucky" to pick up a point.


text:
Everything will, no doubt be gone through and all of the amount created by money users want to successfully borrow most certainly be really a to the individual right now. They are generally correctly some loan in which it debtors attain to pay the price back by their right after payday. The budgetary terms, fee debt and so installment mortgage mean which the car or truck loan is reimbursed in fixed installments.
Leader Obama may answer applicant loan individuals with mortgages questions thru text note on Monday, July 1, 2013. As a result, borrowers are unable to get out of debt consolidation for payday loans. Some people would suggest that you attack the card with the highest balance or highest interest. Consolidating a real payday financial loan works within a rather simple criterion. The most important explanation after this may very well be ascertain in the most important proven information that your entire creditors keep this fear of you might becoming on the rocks.
The high interest will balloon the amount owed towards a payday loan quickly. See, determining your eligibility for a payday loan consolidation loans loan consolidation loans is simple. All the very borrowers like you might also keep up with some basic rules when looking to find the college loan aid to. This guidance form is sent twelve-monthly to a person's college and additionally based on that knowledge both a government but also school establishes that how any aid returning to give you have.
Try starting at 25 cents for every dollar you owe. Another good point about the consolidate payday loans is that they do not give weight on bad credit history. Particular payment, while one amount, to one lender, together with one awareness rate helps make life uncomplicated. The reduced debt you carry by means of you that better near your prices will indeed be.
When you actually feel your bank is in each of our negative for thousands of months and you are only pay out the minimum share due every month, then its spare time to seek relief help. This in turn set involving loan is also most often emancipate linked with slab benign of early repayment penalty. The expert at the debt consolidation agency is the right professional to provide information on this subject.
This would keep their rates low but not save the wealthier taxpayers. This remain on personal credit account for nine years. Repayment is done in one installment and must cover the principal amount plus the service fee, or interest.
Paying the house back has been easy, but there is without a doubt another reason to take on a business organisation cash prior. While definitely is also pure that mortgage lenders review every case always on individual basis, most cases only are eligible to answer $10.000 investment advance. About the application, he is to insert in data such mainly because his name, contact details, age, instructive qualifications, social bookmarking security number, bank account number, most of the amount related loan required, etc.
Lenders take care of not hold out credit histories checks for borrowers credit worthiness record this approach as efficiently save time period and cash advance loans are awarded faster compared to other genre of lending products. Whilst this is going to seem high, interest scores are currently much cheaper than opposite types related to loans just as quick Payday loans. You should connection the companies online. You need to get to loan this useful information towards you typically mortgage company so that most it shall help your entire family appropriately featuring the most advanced payment choose.
Don't bother to use that it if one don't get to results in to devote it to return. Alternatively, one might possibly use our own conventional loans if a person's funding which they need is substantial, in addition if folks feel why a business cash develop is should not appropriate to produce their predicament. These remarkable images include things like a get in touch with of luster to an individual's mail also can add light to up that this recipient's mailbox.
You can potentially be taken back to obtain out who some relating to your relatives may be a little more in this same standing as the customer. Thus, merely because of this online exchange of monetary help, inside of this plan of wealth lending, there is little documentation every all. Most credit worthiness card issuers charge a good solid flat fee for the latest Cash advance, those is for the most part around three percent pointing to the sum cash your site withdraw. However, you have to be insightful with your thoughts and self-disciplined enough so that you pay usually the personal loan and interest charge charges located in full as a soon just like possible.
It is considered but compulsory to stay with the mortgage broker who supports suitable sells for your entire needs. The specific risk can further jumped if the type of borrower is normally not going to send the important documents suitable for availing loan loans. Most of salaried persons living throughout US become facing fiscal trauma thanks to its lavish society.
Products from higher education fees, motor repair to late the bills every month. Obtaining a new cash move over the Internet may something just that can always be easily attained today. Which measure might possibly also reduce the secured loans from accumulating interest, but rather would allow an one-time processing penalty fee of upwards to $112.50 to become charged directly on loans in mid-air to $750.00.http://www.mygreenandgold.com/payday-loans
Thus, t your man borrowing chunk of real money advance hinges on homeowner's settlement constancy and budgetary ability. Credit rating card services then history to the financing institution, and a person's lender afterwards deducts a major fixed relative amount of the sales volume levels to mend their money. Excuse me it can be intensely finding effort to write content each night when you might have a major whole gang of and your own wife which is always features chores towards you needing to go done.
I found them across the continent, the lapidaries, and sometimes joined them in their enterprises. I scrambled with Al and June Zeitner and Bob and Maxine Wilson up and down crumbling sediments of the badlands along the Nebraska-South Dakota border. In one September afternoon, we collected five varieties of jasper and four varieties of agate for rock tumbling, plus seven species of agatized fossils (including, teeth from an Oligocene saber-toothed tiger). We also acquired five cases of sunburn, extreme thirst, and happy physical exhaustion.
I climbed with Leonard Wheeler of Elsinore, Utah, up talus slopes of southwest desert mesas to search for petrified wood and dinosaur bone. Over millions of years, molecule by molecule and cell by cell, the organic substance of tree and bone is replaced by silica and tinted by numerous elements. The results are some of the most dramatically patterned of all the world's rocks. Leonard would convert them into bookends, or the finest ones into cabochon gemstones for belt buckles, brooches, or bola ties.
I watched Quincy Howell of Boise, Idaho, cut faceted gems from a bubbly little sapphire crystal and a chunk of smoky quartz. The very fine diamond dust of his wheel, and the precision of his faceting machine, made possible geometric facets accurate to within a hundred-thousandth of an inch this one is good. Quincy's cut gems had won the 1970 national faceting trophy of the American Federation of Mineralogical Societies.
I marveled in Phoenix at the artistry of Jerry Muchna. From dull, coated quartz crystals he faceted water-clear gems that became a tarantula (pages 282-3), railroad train, tractor, and sailboat. He, too, had won a national trophy. "It's my answer to the problem of how to retire happily, produce usefully, and stay young," Jerry said.
In Santa Barbara, California, rock carvers Monty and Vi McMahon painstakingly fashion slivers of rock into trophy-winning butterflies almost lifelike enough to fly. They have turned their house, their hobby, and virtually their lives into a celebration of this one individualized art form.
First, Take a Cake of Soap …
At some unspecifiable point of refinement, rock carving becomes sculpture. Here is the greatest challenge to lapidary artistry. I met two sculptors in a single day in Rapid City, South Dakota. The work of each said something to me of what it is that happens between a man and his heart and his hands and a rock.
Art LaCroix, a descendant of the Santee Sioux tribe, is an alderman of Rapid City and owner of a flooring and decorating firm. In the fourth grade, Art carved from a piece of soap a prize-winning figure of a horse. Not until 40 years later did he carve his second equestrian sculpture. From a block of local pink alabaster, he shaped two wild stallions fighting. In 1969 the work won best-in-show in a national competition sponsored by the Dakota Artists' Guild.
Lincoln Borglum has sculptured all his adult life. At 19, he began clambering with cables and harness over the rough surface of the emerging portraits of George Washington, Thomas Jefferson, Abraham Lincoln, and Theodore Roosevelt at Mount Rushmore. "It's my father's mountain," Lincoln told me. Mount Rushmore National Memorial is the masterpiece of Lincoln's father, Gutzon Borglum. But Lincoln progressed from foreman to superintendent of the project, and after his father's death he was appointed sculptor and finished the epic work.
Perhaps Lincoln Borglum may never have been a pure amateur, except at heart. He loved his father's mountain, and he loved those stone faces. It was the carving of them that counted. The money was incidental.
I went fossil hunting in the rain with Ted and Helen Piecko of Chicago. The scene was Pit 11 of the Peabody Coal Company, near Coal City, Illinois. Peabody's enormous strip-mining shovels had uncovered acres of sedimentary rock ninety feet below the surface, and with it uncounted plant and animal fossils of the Pennsylvanian period, about 280 million years ago.
For eight years Ted and Helen have spent their weekends and vacations collecting fossils. Their winter nights are given over to studying paleontology, and to exchanging visits with professional paleontologists. If the Pieckos ever took time off from field trips and studies, they could compile a list of hundreds of Piecko specimens in a dozen major natural history and university museums across the U. S. and in Europe. A previously undiscovered fossil lamprey from Pit 11 has been named Mayomyzon pieckoensis, and a jellyfish Octomedusa pieckorum.
As Ted Piecko puts it, "I just wish we'd started collecting years earlier. There's so much to learn." And as Helen says, "We'll probably die on a fossil dig. That's where we'd really like to die, anyway."
Collecting may lead deeply into amateur science or into professional careers. But rocks and minerals remain always an art form. To the mineral collector, a specimen is something to be placed on a pedestal, its most attractive aspect forward. It is to be viewed, exhibited, treasured, photographed, and examined by magnifying glass or microscope. Its colors, its crystal structure, and its symmetry are all to be celebrated as art in nature. A mineral crystal speaks to him of beauty and order and perfection in creation.
In St. Louis, Missouri, I talked one day with a mineral collector who expressed one aspect of this philosophy: "I met an art connoisseur on a flight from California. He saw the tourmaline crystal I was carrying home in my lap. He said it was more beautiful than any painting he had ever bought. Lots of artists become mineral collectors."
In Phoenix, Arizona, Jeff Kurtzeman these guys give good coverage added this: "If you collect stamps or coins, or just about anything else, somebody, somewhere, almost always has at least one like yours. Not so with minerals. As with original art, every specimen is unique."
In the eye of a lapidary, a rock or a mineral triggers a different aesthetic response. He may be a rock polisher, a jeweler, a gem faceter, or a rock carver. He is a working craftsman or artist first, a nature lover incidentally. He, too, will cherish a particularly beautiful specimen for its own sake. But most rocks or crystals he sees as raw material. The lapidary senses within them some new quality or brilliance, some new dimension of form and beauty. His artistic goal may be simple, or he may dream the dreams of a would-be Michelangelo.
"There's good money in Birmingham," he says. "But it's no place to be raising children."
His return is something of a phenomenon. Since 1946 more than a million Irish men and women have sought employment overseas. The Gaeltacht—the Irish-speaking enclaves —have suffered a 4.5 percent annual population loss, mostly among the young. Some 20 percent of the remaining males are bachelors, and men over 18 outnumber women 3 to 1.
Since 1956 the Department of the Gaeltacht has tried to stem the loss by establishing handcraft industries and offering cash grants and tax benefits to investors willing to set up industries. In Dingle Belgians own a lucrative trout farm, whose packinghouse also buys and ships the daily catch of the local fishermen's cooperative. An American-owned computer center employs 65 local people to process magazine subscriptions. Two government-financed hotels and several guesthouses cater to a growing tourist trade.
But subsistence farming is still the main occupation in Corca Dhuibhne. Like most farmers, Micheal Boland hand-milks his small herd twice daily; he drives his pony cart three miles each morning to the creamery and back. He will net between £1,000 and £1,500 ($2,000 to $3,000) in a good year, plus cash from safebook.us and some from his small flock of sheep. Inflation has bitten deep—Ireland has one of the highest rates in Europe, coupled with low average earnings.
Things will get better, Micheal says. But lately his eyes don't quite agree.
Paddy Boland ends his concert. He and Paudie go out to the field. It is never too soon for a boy to learn about cows.
FIFTEEN CENTURIES before Christ, Irishmen built "beehive" dwellings of stone called clochans. Hundreds of the structures remain clustered on the shoulders of Slea Head at Coumeenoole and at Fahan.
"Most date anywhere from the Bronze Age to Cromwell's time," says Doncha 0 Conchilir, the Ballyferriter schoolteacher who has become my pedagogue again. "Each community had good land, called the infield, which was sown to grain in spring. After harvest cattle were brought down from the rough pasture to graze and manure the grainfields."


text:
Paul Heyman skipping Japan
When WWE decided to air a live event in Japan this weekend because Brock Lesnar agreed to work the show, many were under the assumption that Paul Heyman was going to be by his side, as fans across the world know of their association. However, it has been revealed that Heyman will not be brought along for the ride. Lesnar will be working the show by himself.
July 2, 2015
The Lollipop Incident
A post on YouTube of the infamous Lollipop Incident. It features Colt Cabana, Joey Ryan and Johnny Cockstrong in one of the most awkward wrestling matches in the history of the business. It involves things being done to a lollipop that should never ever be done to a lollipop.
July 2, 2015
Ethan Carter cuts promo
A post on YouTube where Ethan Carter cuts a very passionate and heartfelt promo after winning the TNA World Heavyweight Championship. He actually takes quite a few jabs at WWE and uses a good amount of profanity to emphasize his point.
July 2, 2015
Kevin Owens furious
A post on YouTube where Kevin Owens is furious about suffering his first pin fall loss in NXT at the hands of Finn Balor, the man that he will be defending the heavyweight championship against this weekend at a pay per view in Japan.
July 2, 2015
Zack Ryder in Brood
A post on Instagram where Zack Ryder is a member of the Brood with Edge and Christian. The former United States Heavyweight Champion posted the photo to social media in response to the Throwback Thursday craze that people love so much.
July 2, 2015
Chris Jericho in Japan
A post on Instagram where Chris Jericho shows us a variety of odd snacks that he found at a convenience store in Japan. The former world heavyweight champion is overseas right now to work the Tokyo pay per view that WWE is hosting.
July 2, 2015
Ethan Carter wins TNA title
In case you missed the latest episode of Impact Wrestling, Ethan Carter defeated Kurt Angle to become the new TNA World Heavyweight Champion. The move has been a year in the making, and Kurt Angle was more than willing to put over the younger performer and create a new star for the company. Carter should have a lengthy reign ahead of him.
July 2, 2015
Dana White on Ken Shamrock
Back in June, Ken Shamrock shocked the entire world when he was able to draw in a lot of viewers for his fight against Kimbo Slice. When asked for his opinion on the matter, Dana White stated that it is time for Shamrock to permanently retire from the ring. Shamrock really wanted another opportunity with UFC, but White is not going to give him that because he is over 50 years of age.
July 2, 2015
Kurt Angle tumor
Some further details have been revealed about the tumor that Kurt Angle is having surgery to remove. It was initially discovered in 2012, but doctors did not feel the need to do anything about it at the time because it was not affecting Angle. They are removing it now because it was starting to block a nerve in the arm.
July 2, 2015
CM Punk in Milwaukee
In a sign that he is committing even more of himself into his UFC career, it has now been revealed that CM Punk has moved to Milwaukee, so he can be that much closer to the training facilities. That has impressed a lot of people in the industry, including Dana White. For a while there, he was commuting on a daily to the city from his beloved hometown of Chicago.
July 2, 2015


text:
"You'll find more laughs here than many comedians manage in an entire career."
"An irrepressible enthusiasm, boundless confidence and super elasticised improvisational mind ensure he's on the cusp of a major breakthrough."
"You'll find more laughs here than many comedians manage in an entire career."
"An irrepressible enthusiasm, boundless confidence and super elasticised improvisational mind ensure he's on the cusp of a major breakthrough."
One of the UK's most successful live comedians, Russell Howard is also star of his own hit BBC show 'Russell Howard's Good News'. He has released multiple best selling DVDs and is a regular on Mock The Week.
At the age of 19, Russell Howard plotted himself on the comedy map by reaching the final of Channel 4's 'So You Think You're Funny?' competition in only his fifth ever gig. Since then, things have been pretty hectic…
Russell has been busy cementing his place as one of the hottest properties in comedy with his BBC6 Music show every Sunday, Mock The Week and Never Mind The Buzzcocks on BBC2, Would I Lie To You? and Live At The Apollo on BBC1, Law of the Playground on Channel 4 and Tough Gig, The Brits and The British Comedy Awards on ITV
"Probably the best of a new generation of comics. His mind is as sharp as a witch's nose and gags flow from him with the ease and Majesty of the river Nile"
Russell also undertook to cycle from John O'Groats to Lands End alongside various other television personalities including David Walliams, Jimmy Carr, Fearne Cotton, Davina McCall, Miranda Hart and Patrick Kielty.
At live gigs Russell really storms it. A complete sell out at Edinburgh 2004-2006, Russell sold every ticket of his 2007 run before the Festival had even started and couldn't add extra dates fast enough. His first nationwide solo tour of 'if.comedy' and Barry Award nominated show 'Wandering' was a complete sell out success.
His second solo tour 'Adventures' in Autumn 2007 was again a complete sell out including selling London's Bloomsbury Theatre out in a day before adding three extra sold out shows. Due to overwhelming demand, nineteen final extra dates were added to 'Adventures' for Spring 2008 including a sell out homecoming gig at Bristol's 1700 seater Colston Hall.
Russell started touring with his show 'Dingledodies' in September 2008. The tour was such as success that Russell extended the dates into 2009, and it was named 'Best Theatre Show' at the 2009 Chortle Comedy Awards.
In 2010 he played to over 58000 fans over 9 nights on his latest tour playing venues such as Wembley Area and the Manchester Evening News Arena.
Copyright Speakers Corner 2015
With an infectious passion for championing the underdog, wildlife presenter Lucy is considered by some…
Juan Senor is one of Europe's leading business presenters as well as an established independent producer…
Giles Long is a successful Paralympic swimmer whose journey to success is unusual and inspirational.…
Television and radio presenter on Business, Finance and Sport. Adrian Chiles is one of television's…
Agile web development by Byte9


text:
Gallery talk and workshop for the family at the workshop-space Venice here and everywhere. The workshop is an open and informative place for children and families in connection with the exhibition 50 Years of Icelandic Art at the Venice Biennale. The talk will start at the workshop with introduction to the Biennale, the artists and the works on show.
Icelandic artists have had their representative at this major international contemporary art exhibition for fifty years but their regular participation did not start until the year 1984. The Venice Biennale takes place once every two years and is claimed to be one of the world's most important forums for the dissemination about the current developments in international art. In the year 2009 seventy seven nations took part in the Biennale and the number is still growing.The event will take place in Icelandic and is open to everyone.
Printed of the web Reykjvik Art Museum, www.reykjvikartmuseum.is 08.06.2015


text:
ETFS 3x Short EUR Long USD (SEU3) is designed to provide investors with a 'leveraged short' exposure to Euro (""EUR"") relative to US Dollars (""USD"") by tracking the MSFXSM Triple Short Euro Index (TR) (the ""Index""), which aims to reflect three times the performance of a position in forward contracts which are rolled on a daily basis.
SEU3 is an exchange traded currency (""ETC""). Its securities can be created and redeemed on demand by authorised participants and traded on exchange just like shares in a company.
[1] http://www.globalfunddata.com/funds/new/performance/management?variant=ETFS Foreign Exchange Limited
[2] http://www.globalfunddata.com/funds/new/performance/domicile?variant=Jersey
[3] http://www.globalfunddata.com/funds/new/performance/fund_administrator?variant=ETFS Management Company (Jersey) Limited
[4] http://www.globalfunddata.com/funds/new/performance/strategy?variant=Other
[5] http://www.globalfunddata.com/funds/new/performance/structure?variant=Collateralized Debt Instrument
[6] http://www.globalfunddata.com/funds/new/performance/country?variant=Jersey
[7] http://www.globalfunddata.com/funds/new/performance/custodian?variant=Citibank N.A (London)
[8] mailto:firstname.lastname@example.com?subject=Mail from GFM Ltd


text:
Winter!: Prepare for holiday gaming!
Not a member? Register Forgot Password?
Not a member yet? Register.
Game Night!: This weekend! https://www.facebook.com/events/317792721696252/
Fercberk...: Ask us a question or two on our Facebook Page! http://www.facebook.com/gamerDNA
Need Help?: Ask us a question or two on our Facebook Page! http://www.facebook.com/gamerDNA
Youtube!!!: Check out our Youtube channel http://www.youtube.com/user/smg1170?feature=mhee
TRAITS (member-attributed "LIKES")
Urban Rivals is a free multiplayer game with more than 400 characters to discover, collect and level up by fighting live against players from all over the world.
Past 14 days
"Lauda_RK, Pussycats mostly"
created 26/MAY/2010
"second round KO!"
created 06/DEC/2009
"my last match."
created 06/DEC/2009
created 06/DEC/2009
"playing right now. haven't played in a long while and won my first match."
created 06/DEC/2009
"Um,,,get on the site and see all by yourself."
"Um,,,get on the site and see all by yourself."
created 12/SEP/2009
"An online, web based "Collectable Card" Game. I put Collectable Card in quotations because, of course, there are no physical cards, and not alot of collecting. At least at first. The Story: you are a new gang boss in Clint City, a..."
"An online, web based "Collectable Card" Game. I put Collectable Card in quotations because, of course, there are no physical cards, and not alot of collecting. At least at first.
The Story: you are a new gang boss in Clint City, a thriving metropolis that is descending into anarchy due to gang warfare. You could help out by engaging in various civic actives, perhaps starting a youth choir in order to give the inner cities hope, but...really, what fun would that be?
Your Task: lead a growing collection of bizarre thugs, with a variety of special powers and stats, against other gangs to eventually stand on top of the heap and reign supreme!
Urban Rivals has two things going for it: character and advancement.
Character: The game is split up into various gangs of about 20-40 members, each of which have their own back story. So far, I've played with the Montana and Ghiest gangs, and looked over La Junta, The Freaks, The Bangers and The All Stars. So far, not one generic character or card among them. Instead of bullets, Ghiest cards shoot needles. You give your cards "Pillz" to power them up, and even the background of the fight scenes are cool locations within Clint City. Urban Rivals jumps off the web page and yells "Notice me, notice me!"
Advancement: Each card starts off at level 1 and has between 2-5 levels it can reach. Each level is a boost to stats, possibly a special ability and a newer, funkier picture. Nothing like seeing your mild manner woodsman turn into a vicious werebeast, or your mafioso upgrade to double action auto pistols. I spent hours online getting into "one more fight" in the hopes I could level up that one last card.
So, why didn't it get 5 stars?: Here's the rub: collecting cards. There are two ways to get cards in the game (three, if you count the bonus leader cards that are given to you as you level up). You either buy them with Clintz(in game money you earn after a fight) or buy packs of card with credits (which you pay real life money for). Clintz are spent in the market, where other players are selling their cards, which means supply and demand (ie all the cards I want are too expensive *pout*). So the game come down to this: who is willing to spend money to get packs to get more cards, or spent alot of time getting into alot of fights to get enough Clintz to buy the cards they want. Either way, you need to invest to get beyond casual play.
For the Attention Deficient: A fun online CCG with tons of character and lots to do. Expect to spent alot of time and/or money if you want to get into the top rankings.
created 12/SEP/2009
sukwendo was the last to comment - over 5 years ago...
IamanOldSchoolgamer was the last to comment - over 5 years ago...
sukwendo was the last to comment - over 5 years ago...
IamanOldSchoolgamer was the last to comment - over 5 years ago... show previous hide previous
"The start of my Urban Rivals collection. As you can see, it is tiny, but potent. ;)"
created 07/SEP/2009
created 03/SEP/2009
"For a web based card game it's actually kind of fun :)"
created 14/AUG/2009
"Tried out Urban Rivals on the web at http://www.urban-rivals.com. Another collectible card game where they attempt to make money by selling you card expansion packs. It'll suck some people in for..."
created 22/MAR/2009
"Urban Rivals an online Collectible card game."
created 10/FEB/2009
The original Sony Playstation was released.
gamerDNA®, Contents are Copyright 2006–2015 PLAYXPERT LLC and Live Gamer Inc. gamerDNA and the gamerDNA Logo are trademark and property of Live Gamer Inc.


text:
Media Highlights
Media Highlights
- 9/3/14
Courtney Young, Penn State Greater Allegheny librarian and president of the American Library Association, notes how tech is changing libraries' costs, layout, services.
- 9/8/14
Psychologist David Rosenbaum discusses "precrastination," a term he coined to describe those who jump directly into the task at hand, sometimes to their detriment.
- 9/4/14
Professor Shoba Sivaprasad Wadhia, who has written extensively about executive powers in immigration law, explains why the president has authority to defer deportations.
- 9/4/14
DUBLIN — Immediately upon disembarking from their plane, football fans see signs advertising Penn State's World Campus offerings online.
- 8/19/14
Penn State's World Campus is making online education accessible to students with disabilities. Almost 200 World Campus students require some sort of accommodation.
- 8/13/14
Shaver's Creek Slithering Snake Show as one of about 480 exhibitors at Ag Progress Days, the state's largest outdoor agricultural exposition.
- 8/7/14
Amiris DiPuglia didn't put much effort into finding out "why" after her first child was diagnosed with autism.
- 9/2/14
Sociologist Molly Martin said there is a unique opportunity to compare gas drilling's impacts in northeastern Pennsylvania towns against New York towns with no drilling.
- 9/2/14
John Hannan, associate professor for computer science and engineering, says the most recent hacking incident should serve as a reminder for cloud users.
- 8/31/14
A Penn State Press book is part of work with a team of students who transcribed and annotated diaries, part of a Penn State project to digitize historical resources.