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Publications (37)239.93 Total impact
- SourceAvailable from: Michael Way[Show abstract] [Hide abstract]
ABSTRACT: Host innate immune responses to DNA viruses involve members of the nucleotide-binding domain, leucine-rich repeat and pyrin domain containing protein (NLRP) family, which form "inflammasomes" that activate caspase-1, resulting in proteolytic activation of cytokines interleukin (IL)-1β and IL-18. We hypothesized that DNA viruses would target inflammasomes to overcome host defense. A Vaccinia virus (VACV) B-cell CLL/lymphoma 2 (Bcl-2) homolog, F1L, was demonstrated to bind and inhibit the NLR family member NLRP1 in vitro. Moreover, infection of macrophages in culture with virus lacking F1L (ΔF1L) caused increased caspase-1 activation and IL-1β secretion compared with wild-type virus. Virulence of ΔF1L virus was attenuated in vivo, causing altered febrile responses, increased proteolytic processing of caspase-1, and more rapid inflammation in lungs of infected mice without affecting cell death or virus replication. Furthermore, we found that a hexapeptide from F1L is necessary and sufficient for inhibiting the NLRP1 inflammasome in vitro, thus identifying a peptidyl motif required for binding and inhibiting NLRP1. The functional importance of this NLRP1-binding motif was further confirmed by studies of recombinant ΔF1L viruses reconstituted either with the wild-type F1L or a F1L mutant that fails to bind NLRP1. Cellular infection with wild-type F1L reconstituted virus-suppressed IL-1β production, whereas mutant F1L did not. In contrast, both wild-type and mutant versions of F1L equally suppressed apoptosis. In vivo, the NLR nonbinding F1L mutant virus exhibited an attenuated phenotype similar to ΔF1L virus, thus confirming the importance of F1L interactions with NLRP1 for viral pathogenicity in mice. Altogether, these findings reveal a unique viral mechanism for evading host innate immune responses.Proceedings of the National Academy of Sciences 04/2013; DOI:10.1073/pnas.1215995110 · 9.81 Impact Factor
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ABSTRACT: The K7L gene product of the smallpox virus is a protease implicated in the maturation of viral proteins. K7L belongs to protease Clan CE, which includes distantly related cysteine proteases from eukaryotes, pathogenic bacteria, and viruses. Here, we describe its recombinant high-level expression, biochemical mechanism, substrate preference, and regulation. Earlier studies inferred that the orthologous I7L vaccinia protease cleaves at an AG-X motif in six viral proteins. Our data for K7L suggest that the AG-X motif is necessary but not sufficient for optimal cleavage activity. Thus, K7L requires peptides extended into the P7 and P8 positions for efficient substrate cleavage. Catalytic activity of K7L is substantially enhanced by homodimerization, by the substrate protein P25K, as well as by glycerol. RNA and DNA also enhance cleavage of the P25K protein, but not of synthetic peptides, suggesting that nucleic acids augment the interaction of K7L with its protein substrate. Library-based peptide preference analyses enabled us to design an activity-based probe that covalently and selectively labels K7L in lysates of transfected and infected cells. Our study thus provides proof-of-concept for the design of inhibitors and probes that may contribute both to a better understanding of the role of K7L in the virus life cycle and the design of novel anti-virals.Journal of Biological Chemistry 09/2012; DOI:10.1074/jbc.M112.388678 · 4.60 Impact Factor
Article: High-throughput screen for the chemical inhibitors of antiapoptotic bcl-2 family proteins by multiplex flow cytometry.[Show abstract] [Hide abstract]
ABSTRACT: The human Bcl-2 family includes six antiapoptotic members (Bcl-2, Bcl-B, Bcl-W, Bcl-X(L), Bfl-1, and Mcl-1) and many proapoptotic members, wherein a balance between the two determines cell life or death in many physiological and disease contexts. Elevated expression of various antiapoptotic Bcl-2 members is commonly observed in cancers, and chemical inhibitors of these proteins have been shown to promote apoptosis of malignant cells in culture, in animal models, and in human clinical trials. All six antiapoptotic members bind a helix from the proapoptotic family member Bim, thus quenching Bim's apoptotic signal. Here, we describe the use of a multiplex, high-throughput flow cytometry assay for the discovery of small molecule modulators that disrupt the interaction between the antiapoptotic members of the Bcl-2 family and Bim. The six antiapoptotic Bcl-2 family members were expressed as glutathione-S-transferase fusion proteins and bound individually to six glutathione bead sets, with each set having a different intensity of red fluorescence. A fluorescein-conjugated Bcl-2 homology region 3 (BH3) peptide from Bim was employed as a universal ligand. Flow cytometry measured the amount of green peptide bound to each bead set in a given well, with inhibitory compounds resulting in a decrease of green fluorescence on one or more bead set(s). Hits and cheminformatically selected analogs were retested in a dose-response series, resulting in three "active" compounds for Bcl-B. These three compounds were validated by fluorescence polarization and isothermal titration calorimetry. We discuss some of the lessons learned about screening a chemical library provided by the National Institutes of Health Small Molecule Repository (∼195,000 compounds) using high-throughput flow cytometry.Assay and Drug Development Technologies 05/2011; 9(5):465-74. DOI:10.1089/adt.2010.0363 · 2.08 Impact Factor
Article: Mechanism of Bcl-2 and Bcl-X(L) inhibition of NLRP1 inflammasome: loop domain-dependent suppression of ATP binding and oligomerization.[Show abstract] [Hide abstract]
ABSTRACT: NLRP1 (NLR family, pyrin domain-containing 1) is a contributor to innate immunity involved in intracellular sensing of pathogens, as well as danger signals related to cell injury. NLRP1 is one of the core components of caspase-1-activating platforms termed "inflammasomes," which are involved in proteolytic processing of interleukin-1beta (IL-1beta) and in cell death. We previously discovered that anti-apoptotic proteins Bcl-2 and Bcl-X(L) bind to and inhibit NLRP1 in cells. Using an in vitro reconstituted system employing purified recombinant proteins, we studied the mechanism by which Bcl-2 and Bcl-X(L) inhibit NLRP1. Bcl-2 and Bcl-X(L) inhibited caspase-1 activation induced by NLRP1 in a concentration-dependent manner, with K(i) approximately 10 nM. Bcl-2 and Bcl-X(L) were also determined to inhibit ATP binding to NLRP1, which is required for oligomerization of NLRP1, and Bcl-X(L) was demonstrated to interfere with NLRP1 oligomerization. Deletion of the flexible loop regions of Bcl-2 and Bcl-X(L), which are located between the first and second alpha-helices of these anti-apoptotic proteins and which were previously shown to be required for binding NLRP1, abrogated ability to inhibit caspase-1 activation, ATP binding and oligomerization of NLRP1. Conversely, synthetic peptides corresponding to the loop region of Bcl-2 were sufficient to potently inhibit NLRP1. These findings thus demonstrate that the loop domain is necessary and sufficient to inhibit NLRP1, providing insights into the mechanism by which anti-apoptotic proteins Bcl-2 and Bcl-X(L) inhibit NLRP1.Proceedings of the National Academy of Sciences 03/2009; 106(10):3935-40. DOI:10.1073/pnas.0809414106 · 9.81 Impact Factor
Article: Biophysical Mechanism of Converting Apoptosis Regulator Bcl-2 from a Protector to a Killer in Cancer Cells By A Short Nur77-derived PeptideBiophysical Journal 01/2009; 96(3):529. DOI:10.1016/j.bpj.2008.12.2732 · 3.83 Impact Factor
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ABSTRACT: Bcl-2 can be converted into a proapoptotic molecule by nuclear receptor Nur77. However, the development of Bcl-2 converters as anticancer therapeutics has not been explored. Here we report the identification of a Nur77-derived Bcl-2-converting peptide with 9 amino acids (NuBCP-9) and its enantiomer, which induce apoptosis of cancer cells in vitro and in animals. The apoptotic effect of NuBCPs and their activation of Bax are not inhibited but rather potentiated by Bcl-2. NuBCP-9 and its enantiomer bind to the Bcl-2 loop, which shares the characteristics of structurally adaptable regions with many cancer-associated and signaling proteins. NuBCP-9s act as molecular switches to dislodge the Bcl-2 BH4 domain, exposing its BH3 domain, which in turn blocks the activity of antiapoptotic Bcl-X(L).Cancer cell 11/2008; 14(4):285-98. DOI:10.1016/j.ccr.2008.09.002 · 23.89 Impact Factor
Article: A TR3/Nur77 peptide-based high-throughput fluorescence polarization screen for small molecule Bcl-B inhibitors.[Show abstract] [Hide abstract]
ABSTRACT: Nuclear receptor TR3/Nur77/NR4A1 binds several antiapoptotic Bcl-2-family proteins (Bcl-B, Bcl-2, Bfl-1) in a non-BH3-dependent manner. A 9-amino-acid peptide derived from full-length TR3 with polyarginine tail (TR3-r8) recapitulates TR3's binding specificity, displaying high affinity for Bcl-B. TR3-r8 peptide was used to screen for small molecule Bcl-B inhibitors. A fluorescence polarization assay (FPA) employing fluorescein isothiocyanate (FITC)-labeled TR3-r8 peptide (FITC-TR3-r8) and Bcl-B protein was optimized, with nonfluorescent TR3-r8 serving to demonstrate reversible, competitive binding. Approximately 50,000 compounds were screened at 3.75 mg/L, yielding 145 reproducible hits with > or =50% FITC-TR3-r8 displacement (a confirmed hit rate of 0.29%). After dose-response analyses and counterscreening with an unrelated FITC-based FPA, 6 candidate compounds remained. Nuclear magnetic resonance (NMR) showed that 2 of these compounds bound Bcl-B, but not glutathione S-transferase (GST) control protein. One Bcl-B-binding compound was unable to displace FITClabeled BH3 peptides from Bcl-B, confirming a unique binding mechanism compared with traditional antagonists of antiapoptotic Bcl-2-family proteins. This compound bound Bcl-B with Kd 1.94 +/- 0.38 microM, as determined by isothermal titration calorimetry. Experiments using Bcl-B overexpressing HeLa cells demonstrated that this compound induced Bcl-B-dependent cell death. The current FPA represents a screen that can identify noncanonical inhibitors of Bcl-2-family proteins.Journal of Biomolecular Screening 07/2008; 13(7):665-73. DOI:10.1177/1087057108320918 · 2.01 Impact Factor
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ABSTRACT: The natural product gambogic acid (GA) has been reported to have cytotoxic activity against tumor cells in culture and was identified as an active compound in a cell-based high-throughput screening assay for activators of caspases, proteases involved in apoptosis. Using the antiapoptotic Bcl-2 family protein, Bfl-1, as a target for screening of a library of natural products, we identified GA as a competitive inhibitor that displaced BH3 peptides from Bfl-1 in a fluorescence polarization assay. Analysis of competition for BH3 peptide binding revealed that GA inhibits all six human Bcl-2 family proteins to various extents, with Mcl-1 and Bcl-B the most potently inhibited [concentrations required for 50% inhibition (IC(50)), < 1 micromol/L]. Competition for BH3 peptide binding was also confirmed using a time-resolved fluorescence resonance energy transfer assay. GA functionally inhibited the antiapoptotic Bcl-2 family proteins as shown by experiments using isolated mitochondria in which recombinant purified Bcl-2 family proteins suppress SMAC release in vitro, showing that GA neutralizes their suppressive effects on mitochondria in a concentration-dependent manner. GA killed tumor cell lines via an apoptotic mechanism, whereas analogues of GA with greatly reduced potency at BH3 peptide displacement showed little or no cytotoxic activity. However, GA retained cytotoxic activity against bax-/-bak-/- cells in which antiapoptotic Bcl-2 family proteins lack a cytoprotective phenotype, implying that GA also has additional targets that contribute to its cytotoxic mechanism. Altogether, the findings suggest that suppression of antiapoptotic Bcl-2 family proteins may be among the cytotoxic mechanisms by which GA kills tumor cells.Molecular Cancer Therapeutics 06/2008; 7(6):1639-46. DOI:10.1158/1535-7163.MCT-07-2373 · 6.11 Impact Factor
Article: Differential regulation of Bax and Bak by anti-apoptotic Bcl-2 family proteins Bcl-B and Mcl-1[Show abstract] [Hide abstract]
ABSTRACT: The pro-apoptotic members of the Bcl-2 family include initiator proteins that contain only BH3 domains and downstream effector multi-BH domain-containing proteins, including Bax and Bak. In this report, we compared the ability of the six human anti-apoptotic Bcl-2 family members to suppress apoptosis induced by overexpression of Bax or Bak, correlating findings with protein interactions measured by three different methods: co-immunoprecipitation, glutathione S-transferase pulldown, and fluorescence polarization assays employing synthetic BH3 peptides from Bax and Bak. Bcl-B and Mcl-1 showed strong preferences for binding to and suppression of Bax and Bak, respectively. In contrast, the other anti-apoptotic Bcl-2 family proteins (Bcl-2, Bcl-X(L), Bcl-W, and Bfl-1) suppressed apoptosis induced by overexpression of either Bax or Bak, and they displayed an ability to bind both Bax and Bak by at least one of the three protein interaction methods. Interestingly, however, full-length Bax and Bak proteins and synthetic Bax and Bak BH3 peptides exhibited discernible differences in their interactions with some anti-apoptotic members of the Bcl-2 family, cautioning against reliance on a single method for detecting protein interactions of functional significance. Altogether, the findings reveal striking distinctions in the behaviors of Bcl-B and Mcl-1 relative to the other anti-apoptotic Bcl-2 family members, where Bcl-B and Mcl-1 display reciprocal abilities to bind and neutralize Bax and Bak.Journal of Biological Chemistry 05/2008; 283(15):9580-6. DOI:10.1074/jbc.M708426200 · 4.60 Impact Factor
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ABSTRACT: RIZ1 is a transcriptional regulator and tumor suppressor that catalyzes methylation of lysine 9 of histone H3. It contains a distinct SET domain, sometimes referred to as PR (PRDI-BF1 and RIZ1 homology) domain, that is responsible for its catalytic activity. We determined the solution structure of the PR domain from RIZ1 and characterized its interaction with S-adenosyl-l-homocysteine (SAH) and a peptide from histone H3. Despite low sequence identity with canonical SET domains, the PR domain displays a typical SET fold including a pseudo-knot at the C-terminus. The N-flanking sequence of RIZ1 PR domain adopts a novel conformation and interacts closely with the SET fold. The C-flanking sequence contains an alpha-helix that points away from the protein face that harbors active site in other SET domains. The SET fold of RIZ1 does not have detectable affinity for SAH but it interacts with a synthetic peptide comprising residues 1-20 of histone H3.Biochemical and Biophysical Research Communications 03/2008; 366(3):807-13. DOI:10.1016/j.bbrc.2007.12.034 · 2.28 Impact Factor
Article: Mapping the specific cytoprotective interaction of humanin with the pro-apoptotic protein bid.[Show abstract] [Hide abstract]
ABSTRACT: Humanin is a short endogenous peptide, which can provide protection from cell death through its association with various receptors, including the pro-apoptotic Bcl-2 family proteins Bid, Bim, and Bax. By using NMR chemical shift mapping experiments, we demonstrate that the interaction between Humanin-derived peptides and Bid is specific, and we localize the binding site to a region on the surface of Bid, which includes residues from the conserved helical BH3 domain of the protein. The BH3 domain mediates the association of Bid with other Bcl-2 family members and is essential for the protein's cytotoxic activity. The data suggest that Humanin exerts its cytoprotective activity by engaging the Bid BH3 domain; this would hinder the association of Bid with other Bcl-2 family proteins, thereby mitigating its toxicity. The identification of a Humanin-specific binding site on the surface of Bid reinforces its importance as a direct modulator of programmed cell death, and suggests a strategy for the design of cytoprotective peptide inhibitors of Bid.Chemical Biology & Drug Design 12/2007; 70(5):383-92. DOI:10.1111/j.1747-0285.2007.00576.x · 2.51 Impact Factor
Article: Nur77 converts phenotype of Bcl-B, an antiapoptotic protein expressed in plasma cells and myeloma[Show abstract] [Hide abstract]
ABSTRACT: Defects in apoptosis mechanisms play important roles in malignancy and autoimmunity. Orphan nuclear receptor Nur77/TR3 has been demonstrated to bind antiapoptotic protein Bcl-2 and convert it from a cytoprotective to a cytodestructive protein, representing a phenotypic conversion mechanism. Of the 6 antiapoptotic human Bcl-2 family members, we found that Nur77/TR3 binds strongest to Bcl-B, showing selective reactivity with Bcl-B, Bcl-2, and Bfl-1 but not Bcl-X(L), Mcl-1, or Bcl-W. Nur77 converts the phenotype of Bcl-B from antiapoptotic to proapoptotic. Bcl-B is prominently expressed in plasma cells and multiple myeloma. Endogenous Bcl-B associates with endogenous Nur77 in RPMI 8226 myeloma cells, where RNA interference experiments demonstrated dependence on Bcl-B for Nur77-induced apoptosis. Furthermore, a Nur77-mimicking peptide killed RPMI 8226 myeloma cells through a Bcl-B-dependent mechanism. Because Bcl-B is abundantly expressed in plasma cells and some myelomas, these findings raise the possibility of exploiting the Nur77/Bcl-B mechanism for apoptosis for eradication of autoimmune plasma cells or myeloma.Blood 06/2007; 109(9):3849-55. DOI:10.1182/blood-2006-11-056879 · 10.43 Impact Factor
- Cell Death and Differentiation 09/2006; 13(8):1419-21. DOI:10.1038/sj.cdd.4401937 · 8.39 Impact Factor
Article: Anthrax lethal factor protease inhibitors: Synthesis, SAR, and structure-based 3D QSAR studies[Show abstract] [Hide abstract]
ABSTRACT: We have recently identified a series of compounds that efficiently inhibit anthrax lethal factor (LF) metallo-protease. Here we present further structure-activity relationship and CoMFA (comparative molecular field analysis) studies on newly derived inhibitors. The obtained 3D QSAR model was subsequently compared with the X-ray structure of the complex between LF and a representative compound. Our studies form the basis for the rational design of additional compounds with improved activity and selectivity.Journal of Medicinal Chemistry 02/2006; 49(1):27-30. DOI:10.1021/jm050892j · 5.48 Impact Factor
Article: LMP1 protein from the Epstein-Barr virus is a structural CD40 decoy in B lymphocytes for binding to TRAF3[Show abstract] [Hide abstract]
ABSTRACT: Epstein-Barr virus is a human herpesvirus that causes infectious mononucleosis and lymphoproliferative malignancies. LMP1 (latent membrane protein-1), which is encoded by this virus and which is essential for transformation of B lymphocytes, acts as a constitutively active mimic of the tumor necrosis factor receptor (TNFR) CD40. LMP1 is an integral membrane protein containing six transmembrane segments and a cytoplasmic domain at the C terminus that binds to intracellular TNFR-associated factors (TRAFs). TRAFs are intracellular co-inducers of downstream signaling from CD40 and other TNFRs, and TRAF3 is required for activation of B lymphocytes by LMP1. Cytoplasmic C-terminal activation region 1 of LMP1 bears a motif (PQQAT) that conforms to the TRAF recognition motif PVQET in CD40. In this study, we report the crystal structure of this portion of LMP1 C-terminal activation region-1 (204PQQATDD210) bound in complex with TRAF3. The PQQAT motif is bound in the same binding crevice on TRAF3 where CD40 is bound, providing a molecular mechanism for LMP1 to act as a CD40 decoy for TRAF3. The LMP1 motif is presented in the TRAF3 crevice as a close structural mimic of the PVQET motif in CD40, and the intermolecular contacts are similar. However, the viral protein makes a unique contact: a hydrogen bond network formed between Asp210 in LMP1 and Tyr395 and Arg393 in TRAF3. This intermolecular contact is not made in the CD40-TRAF3 complex. The additional hydrogen bonds may stabilize the complex and strengthen the binding to permit LMP1 to compete with CD40 for binding to the TRAF3 crevice, influencing downstream signaling to B lymphocytes and contributing to dysregulated signaling by LMP1.Journal of Biological Chemistry 10/2005; 280(39):33620-6. DOI:10.1074/jbc.M502511200 · 4.60 Impact Factor
Article: Cytoprotective peptide humanin binds and inhibits proapoptotic Bcl-2/Bax family protein BimEL[Show abstract] [Hide abstract]
ABSTRACT: Humanin (HN) is a recently identified endogenous peptide that protects cells against cytotoxicity induced by various stimuli. Recently, we showed that HN binds to and inhibits Bax, a proapoptotic Bcl-2 family protein, suggesting a mechanism for HN action. In this study, we identified Bim, a Bcl-2 homology 3-only member of the Bcl-2/Bax family, as an additional HN target protein. Using in vitro protein binding, immunoprecipitation, and coimmunolocalization assays, we demonstrated that HN binds directly to the extra long isoform of Bim (BimEL) but not the long (BimL) or short (BimS) isoforms. HN also protects cells against apoptosis induced by BimEL but not BimL and BimS in gene transfection studies. In contrast, mutants of HN which failed to bind BimEL failed to protect from BimEL-induced cell death. Moreover, HN inhibited BimEL-induced release of SMAC and cytochrome c from mitochondria isolated from bax-/-cells, indicating that HN can suppress BimEL independently of its effect on Bax. Finally, we demonstrate that HN prevents BimEL-induced oligomerization of Bak using isolated mitochondria. Taken together, our results indicate that the inhibition of BimEL may contribute to the antiapoptotic properties of the HN peptide.Journal of Biological Chemistry 05/2005; 280(16):15825-35. DOI:10.1074/jbc.M413062200 · 4.60 Impact Factor
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ABSTRACT: Recently, we discovered that Humanin (HN), a small endogenous peptide of 24 amino acids, binds to and inhibits the proapoptotic protein Bax. We show here that HN also interacts with the BH3-only Bcl-2/Bax family protein, Bid, as well as a truncated form of Bid (tBid) associated with protease-mediated activation of this proapoptotic protein. Synthetic HN peptide binds purified Bid and tBid in vitro and blocks tBid-induced release of cytochrome c and SMAC from isolated mitochondria, whereas mutant peptides that fail to bind Bid or tBid lack this activity. Moreover, HN peptide also retained protective activity on bax-/-mitochondria, indicating that HN can block tBid-induced release of apoptogenic proteins from these organelles in a Bax-independent manner. HN peptide inhibits tBid-induced oligomerization of Bax and Bak in mitochondrial membranes, as shown by experiments with chemical cross-linkers or gel filtration. Gene transfection experiments showed that HN (but not an inactive mutant of HN) also protects intact cells from apoptosis induced by overexpression of tBid. We conclude that Bid represents an additional cellular target of HN, and we propose that HN-mediated suppression of Bid contributes to the antiapoptotic activity of this endogenous peptide.Journal of Biological Chemistry 05/2005; 280(16):15815-24. DOI:10.1074/jbc.M411902200 · 4.60 Impact Factor
Article: Key molecular contacts promote recognition of the BAFF receptor by TNF receptor-associated factor 3: implications for intracellular signaling regulation.[Show abstract] [Hide abstract]
ABSTRACT: B cell-activating factor belonging to the TNF family receptor (BAFF-R), a member of the TNFR superfamily, plays a role in autoimmunity after ligation with BAFF ligand (also called TALL-1, BLyS, THANK, or zTNF4). BAFF/BAFF-R interactions are critical for B cell regulation, and signaling from this ligand-receptor complex results in NF-kappaB activation. Most TNFRs transmit signals intracellularly by recruitment of adaptor proteins called TNFR-associated factors (TRAFs). However, BAFF-R binds only one TRAF adaptor, TRAF3, and this interaction negatively regulates activation of NF-kappaB. In this study, we report the crystal structure of a 24-residue fragment of the cytoplasmic portion of BAFF-R bound in complex with TRAF3. The recognition motif (162)PVPAT(166) in BAFF-R is accommodated in the same binding crevice on TRAF3 that binds two related TNFRs, CD40 and LTbetaR, but is presented in a completely different structural framework. This region of BAFF-R assumes an open conformation with two extended strands opposed at right angles that each make contacts with TRAF3. The recognition motif is located in the N-terminal arm and intermolecular contacts mediate TRAF recognition. In the C-terminal arm, key stabilizing contacts are made, including critical hydrogen bonds with Gln(379) in TRAF3 that define the molecular basis for selective binding of BAFF-R solely to this member of the TRAF family. A dynamic conformational adjustment of Tyr(377) in TRAF3 occurs forming a new intermolecular contact with BAFF-R that stabilizes the complex. The structure of the complex provides a molecular explanation for binding affinities and selective protein interactions in TNFR-TRAF interactions.The Journal of Immunology 01/2005; 173(12):7394-400. DOI:10.4049/jimmunol.173.12.7394 · 5.36 Impact Factor
Article: Structurally distinct recognition motifs in lymphotoxin-beta receptor and CD40 for tumor necrosis factor receptor-associated factor (TRAF)-mediated signaling[Show abstract] [Hide abstract]
ABSTRACT: Lymphotoxin-beta receptor (LTbetaR) and CD40 are members of the tumor necrosis factor family of signaling receptors that regulate cell survival or death through activation of NF-kappaB. These receptors transmit signals through downstream adaptor proteins called tumor necrosis factor receptor-associated factors (TRAFs). In this study, the crystal structure of a region of the cytoplasmic domain of LTbetaR bound to TRAF3 has revealed an unexpected new recognition motif, 388IPEEGD393, for TRAF3 binding. Although this motif is distinct in sequence and structure from the PVQET motif in CD40 and PIQCT in the regulator TRAF-associated NF-kappaB activator (TANK), recognition is mediated in the same binding crevice on the surface of TRAF3. The results reveal structurally adaptive "hot spots" in the TRAF3-binding crevice that promote molecular interactions driving specific signaling after contact with LTbetaR, CD40, or the downstream regulator TANK.Journal of Biological Chemistry 01/2004; 278(50):50523-9. DOI:10.1074/jbc.M309381200 · 4.60 Impact Factor
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ABSTRACT: Bax (Bcl2-associated X protein) is an apoptosis-inducing protein that participates in cell death during normal development and in various diseases. Bax resides in an inactive state in the cytosol of many cells. In response to death stimuli, Bax protein undergoes conformational changes that expose membrane-targeting domains, resulting in its translocation to mitochondrial membranes, where Bax inserts and causes release of cytochrome c and other apoptogenic proteins. It is unknown what controls conversion of Bax from the inactive to active conformation. Here we show that Bax interacts with humanin (HN), an anti-apoptotic peptide of 24 amino acids encoded in mammalian genomes. HN prevents the translocation of Bax from cytosol to mitochondria. Conversely, reducing HN expression by small interfering RNAs sensitizes cells to Bax and increases Bax translocation to membranes. HN peptides also block Bax association with isolated mitochondria, and suppress cytochrome c release in vitro. Notably, the mitochondrial genome contains an identical open reading frame, and the mitochondrial version of HN can also bind and suppress Bax. We speculate therefore that HN arose from mitochondria and transferred to the nuclear genome, providing a mechanism for protecting these organelles from Bax.Nature 06/2003; 423(6938):456-61. DOI:10.1038/nature01627 · 42.35 Impact Factor
Top Journals
Sanford-Burnham Medical Research Institute
La Jolla, California, United States
- • Conrad Prebys Center for Chemical Genomics
- • Infectious and Inflammatory Disease Center
University of Iowa
Iowa City, Iowa, United States
- Department of Internal Medicine
La Jolla Institute for Allergy & Immunologyلا هویا, California, United States
Pacific Northwest National LaboratoryРичленд, Washington, United States
The Scripps Research Institute
لا هویا, California, United States
- Department of Cell and Molecular Biology
NYU Langone Medical CenterNew York, New York, United States


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1170 Klockner Road
Hamilton Township
Commute to Downtown Trenton
Most errands require a car.
Score Details
1170 Klockner Road has a Walk Score of 48 out of 100. This location is a Car-Dependent neighborhood so most errands require a car.
The closest park is Bromley Park.
Explore how far you can travel by car, bus, bike and foot from 1170 Klockner Road.
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subset: commoncrawl


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iHerb 顾客评论
产品 1-4,共 4 个产品
Not a good Ranch Dressing Flavor
1 个顾客认为以下的评论有帮助
Reviewer2263045发表于 Jun 19, 2012
I was disappointed in this salad dressing. It is useable if I add some chipotle powder to it. By itself, it is oddly seasoned, a bit sweet, and in no way represents the flavor of any Ranch Dressing I have ever had. I will go back to my usual homemade with Greek yogurt and mayo.
Funny After Taste
1 个顾客认为以下的评论有帮助
Reviewer3086632发表于 Dec 26, 2011
I did not like this at all. It had a funny after taste and did not taste like ranch.
Herlienv发表于 May 19, 2015
always a winner
Cowgirl Ranch Dressing
Reviewer2672073发表于 Dec 04, 2012
Good dressing. Will order again in the near future.
产品 1-4,共 4 个产品


text:
All Firearms
Gun Parts
|Seller's Description
This is a Beretta Model 84 Double Action, Double Stack Pistol in 380 Auto. This is an exceptionally nice carry piece to go with your concealed carry license. It has ambidextrous safeties, a double stack magazine, double action firing, walnut grips and very good sights. It has been fired, but looks new in the box with the manual. FFL required. Buyer pays $20.00 shipping. Certified funds please. Phone is 251-928-0108.
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'The Big Bang Theory' Still Most Watched Show on Canadian TV
The nerdy CBS comedy even gave CTV a rare Saturday night win as it airs in repeats as a comedy block against a diminished "Hockey Night in Canada" during the NHL lockout.
TORONTO – The Big Bang Theory remains Canada's most-watched TV show, and earned domestic broadcaster CTV a rare Saturday night win.
An average 4.5 million Canadians have so far tuned into the CBS comedy each week during fall 2012, according to BBM Canada data, up 18 percent compared to fall 2011 when The Big Bang Theory also topped viewership tables here.
PHOTOS: Behind the Scenes of 'The Big Bang Theory'
And the nerdy comedy helped CTV win Saturday night last weekend when CTV scheduled a Big Bang Night on CTV comedy block opposite Hockey Night in Canada on the CBC during the NHL lockout.
The repeats of Big Bang Theory on October 13 drew 1.24 million viewers, well ahead of the 293,000 viewers that watched a repeat of the May 10, 1979 playoff game between the Boston Bruins and the Montreal Canadiens.
Hockey Night in Canada, a national institution on the CBC, routinely draws upwards of 2 million viewers when airing live NHL games.
But the hockey league's work stoppage has forced the CBC to air repeat games to preserve its audience and ad revenue.
And that vacuum has produced an opening for CTV to command Saturday nights north of the border, when it otherwise dominates weeknights with its stable of U.S. network series.
STORY: Canadian Nets Clash Over 'The Big Bang Theory' Promotion During NHL Lockout
CTV's other fall 2012 performers include Grey's Anatomy and Two and a Half Men with an average 2.9 million viewers each, both up over last year, and Arrow, which debuted on CTV Two to 1.1 million viewers.
CTV also has the Charlie Sheen-starrer Anger Management on its schedule.
Never miss a story. Stay up-to-date with THR's Daily Headlines.
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VIEWS: 9 PAGES: 2 POSTED ON: 5/3/2011 Public Domain
?Link building is essential in order to rank a site on the search engine. There are two types of links including one way links and two way links. Two way links is done by exchanging links with each another site. Link exchange used to be effective in the past. However, many webmasters abuse it by link exchange with unrelated site that are spammy. As a result, Google had decrease the value of link exchange. Nowadays, one way link is the best way to build backlinks for your site. One way to obtain free one way links is through social bookmarking. Social bookmarking site is a site that allows users to store and share their web pages with other people on the internet. Webmasters can take advantage of social bookmarking site to promote their website. By submitting to the social bookmarking site, you will obtain a free one way link to your site. You can submit 1 - 3 links per day. You should not attempt to social bookmark too many post as it will attract the attention of other users. When you submit a post, it will appear on top of the search result in the recent bookmark page. If you keep submitting your page, the search result will be filled with your bookmarks on your website. When people visit the Recent bookmarks page, they will not be able to browse the content which they are looking for. Therefore, the user will resort to report you to the moderator. Once the moderator is notified, he will perform an investigation and ban your account. The links displayed on the social bookmarking sites can be dofollow or nofollow. Dofollow means the link will be crawled and indexed by the search engine. Nofollow means that the search engine will ignore the link as if it is not there. Nofollow link has no SEO benefits. When submitting to social bookmarking site, make sure to check if it is dofollow or nofollow. In this way, you won't waste your time submitting to a nofollow social bookmarking site. You can find social bookmarking site by performing a search on the search engine. For example, you can enter the keyword "top social bookmarking sites" in the search box of the search engine such as Google. After you hit enter, you will see a list of result that offer the top social bookmaking site list. You can bookmark the social bookmaking site so that you can visit it in the future submission. There are more than one hundred social bookmarking sites on the internet. In addition, there are hundreds of social bookmarking sites that are based on the Pligg platform. With so many social bookmarking sites, it can be time consuming to submit to all of them. If you don't have time to submit to them, you can hire a SEO company to perform the social bookmarking submission for you. The cost for the social bookmarking submission is cheap and affordable. Before hiring a SEO company, make sure you check their portfolio to see if they can handle the job properly Mascar Rooney is the author of this article on Blog Commenting Service. Mascar Rooney is the author of this article on Dmoz guide.
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text: Tom Hardy Born Sep 15, 1977 Hammersmith, London, England Age 37 Tom Hardy had his breakthrough performance in Inception and has acted in other mainstream films since including The Dark Night Rises. Get Alerts about Tom Hardy By signing up, I agree to the Terms and to receive emails from POPSUGAR. View 226 photos of Tom Hardy » Start Slideshow | View Gallery Posts View 5 videos of Tom Hardy »


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Peggy turns the Big Two today, and to celebrate she's givin' us some MAJOR Princess Di Side-Eye! "She makes us laugh every day," says Mary H. Can't imagine why. :)
Peggy turns the Big Two today, and to celebrate she's givin' us some MAJOR Princess Di Side-Eye! "She makes us laugh every day," says Mary H. Can't imagine why. :)
Ever see a photo on Cute Overload and go, "I don't care where he/she is, I am getting in a car/on a plane to go see him/her NOW!" That's what I just decided with Boomer. (Oh, and before I forget, nice Side-Eye up there, too.)
"I just wanted to submit Boomer, our Labradoodle Puppeh. He's just over a year old right now."
"We've been wanting to submit him ever since his brother-in-law (Nermal, the British short-hair) got featured on your website… twice!
"Hope he makes the cut!"
"Photos were taken by my fiance ('Izzie') and I." -John W.
Morning coffee…AND CUTE BEBEH PYGMY GOATS! Gaaaaaaaaaaaaaa. Saw this one on T.O.
Tiny Tongues are Cute. But then we also have #99: Peeping Tongues Are Cute, too. Aren't they pretty much the same thing? This Red Panda photo is from Flickr user Mark Dumont.
After you clean up your desk or wherever from The Mandatory Head 'Splosion, don't forget to consult Rule Of Cuteness #45: Any animal with the word "pygmy" in it is cute.
People, meet Meet Millie (aka Stampy.)
From Kimberly K.
Earlier today, we saw BFFs Morris and Champy hanging out. Now we get a sequel of sorts! "This is Moo Pony and his new cat friend," from Submitter Kerry D.
This little pupster is named "Holly." Let's check 'er out from a few different angles, shall we? The second photo below is for scale. As you can see, Holly is quite the teeny lass.
And in this next one, Holly is so Fuzzular (new word) that she kinda resembles a…gold carpet sample.
And finally, we're getting some Wicked Prosh Side-Eye (RoC #95) in this next one.
Stacey K. reports, "These photos were taken by Holly's adoring owner, Katie S."
You almost never see a Kitteh do a Baroo, because….they're Kittehs, and they do what they want. This concept continues through adulthood, as well all know. We DO have one here, though- c/o First-Time Submitter Mēgan O. "Hullo! Never submitted before, but LOVE the site. Taj, my 6 month old boy, stands stock-still under the Christmas Tree, almost in a trance sometimes. A friend of mine snapped this and said he looks like the E.T. of kittehs."
"I've been a huge fan of Cute Overload since the day I emailed Cody's puppy pictures to a friend who replied that Cody was as cute as anybody on C.O., and I should visit the site immediately, and submit a photo someday….which took me a while, but here it is. Just in case you don't have enough photos in the category "pups humoring humans for a Christmas card shot," here's one of our girl Cody doing an awfully good job of it. She is a 3-year old lab mix. We'd love to see her on Cute Overload!" -Nora & Glenn.
Some Rules Of Cuteness just jump right out at ya. Ahem, if you will- presenting RoC 97: "Head To Body Ratio: If your head is exponentially larger than the rest of your entire body, that's Cute."
"This is Chloe. She just turned "fo weeks ode". She has decided that this is an appropriate time to share her astonishing Cuteness with the world. So here, dear Cute Overload, is Miss Chloe. Sent in by her smitten human, Donna."
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Gender Events
Expanding Access to Essential Maternal Health Commodities
November 30, 2010 // 8:30am — 10:30am
Maternal Health Initiative
The ninth meeting of the 2009-2010 Advancing Policy Dialogue on Maternal Health series addresses the challenges and strategies for expanding access to maternal health commodities.
Paradise Beneath Her Feet: How Women Are Transforming the Middle East
October 27, 2010 // 4:00pm — 5:00pm
Middle East Program
Isobel Coleman of the Council on Foreign Relations highlights how demographic changes are intensifying the notion that women's empowerment is key to the prosperity of the economies of Arab and Muslim-majority countries.
New Applications for Existing Technologies to Improve Maternal Health
October 27, 2010 // 3:00pm — 5:00pm
Maternal Health Initiative
Josh Nesbit, Alain Labrique, and David Aylward discuss the role of Information and Communication Technologies in the prevention of maternal mortality.
A Modern Narrative for Muslim Women in the Middle East
September 30, 2010 // 10:00am — 12:00pm
Middle East Program
Fawziah Al-Hani, Manager, Gherass Center for Social Education, Saudi Arabia; Rana Husseini, Human Rights Activist, Author and Journalist, Jordan; Zainab Al-Suwaij,Executive Director, American Islamic Congress
Demography and Women's Empowerment: Urgency for Action?
September 13, 2010 // 12:00pm — 1:00pm
Middle East Program
Middle Eastern women should be empowered to participate in the economy in a more significant way if the region is to take advantage of its "demographic window of opportunity," says the World Bank's Nadereh Chamlou.
Improving Monitoring, Transparency, and Accountability for Maternal, Newborn, and Child Health
September 08, 2010 // 10:00am — 12:00pm
Maternal Health Initiative
Panelists discuss strategies for improving maternal health evaluation methods while balancing the interests of donors and beneficiaries during the seventh meeting of the Advancing Policy Dialogue on Maternal Health series.
Women in Developing Countries: Sowing the Seeds for the Future
July 30, 2010 // 12:00pm — 1:15pm
Wilson Center on the Hill
Two leading experts detail the challenges and opportunities for women in developing countries, as well as how the United States can create a new, more effective development policy that recognizes the key role of women.
The Impact of Maternal Mortality and Morbidity on Economic Development
July 29, 2010 // 3:00pm — 5:00pm
Maternal Health Initiative
The sixth meeting of the Advancing Policy Dialogue on Maternal Health Series addresses the economic impact of maternal mortality, and provides evidence of the need for increased investment in maternal health.
In the Midst of Milestones: Perspectives from Afghan Women Leaders
June 17, 2010 // 10:00am — 11:00am
Middle East Program
Huria Samira Hamidi, Afghan Women's Network; Najla Ayubi, Organization of Strategic Studies and Research
Islamic Feminism and Beyond: The New Frontier
June 15, 2010 // 8:30am — 1:00pm
Middle East Program
Amaney Jamal, Assistant Professor, Department of Politics, Princeton University; Nayereh Tohidi, Professor, Gender and Women's Studies, California State University, Northridge; Lilia Labidi, Professor, Anthropology and Psychology, University of Tunis; Haleh Esfandiari, Director, Middle East Program, Woodrow Wilson Center; Margot Badran, Senior Scholar, Woodrow Wilson Center; Senior Fellow, Prince Alwaleed bin Talal Center for Muslim Christian Understanding, Georgetown University; Souad Eddouada, Assistant Professor, Department of English, Ibn Tofail University, Kenitra, Morocco; Binnaz Toprak, Professor, Political Science, Department of Political Science and International Relations, Bahçesehir University, Istabul; Zainab Al-Suwaij, Executive Director, American Islamic Congress


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American Rhythm Center to Host Free Classes, 9/8-13
Kicking off its third year of offering Chicago a range of dance classes at its innovative, collaborative dance/arts facility, the American Rhythm Center (ARC), a program of the Chicago Human Rhythm Project (CHRP), hosts a Free 4 All, six days of free dance classes on the third floor of the historic Fine Arts Building, 410 S. Michigan Avenue. The event takes place Monday-Saturday, September 8-13, 2014.
The Free 4 All features free classes for all ability levels in a range of genres and styles, including tap, flamenco, Zumba, modern, hip hop, Tai Chi and more. Teaching the classes are professionals and young dancers representing the ARC's community partners and other affiliated groups, including Chicago Human Rhythm Project, Cerqua Rivera Dance Theatre, Chicago Chinese Cultural Institute, DMX Dance Company, Ensemble Español Spanish Dance Theater, Esoteric Dance Project, Gang of Toes, Kalapriya Center for Indian Performing Arts, M.A.D.D. Rhythms, MPulse, Muntu Dance Theatre, Movement Revolution Dance Crew and more.
On Friday, September 12, the ARC hosts a free Improvisation Jam at 6 p.m. for modern and contemporary dancers and a free Tap Jam for hoofers and tap dancers at 8 p.m. Donations are welcome.
The ARC is Chicago Human Rhythm Project's collaborative initiative to provide a shared, affordable and sustainable education, rehearsal and administrative facility for several leading Chicago arts organizations. The aim is to offer diverse, high-quality dance and movement classes to the general public while centralizing the education programs, rehearsal space and administrative offices of several core groups, as well as additional emerging companies and independent artists. The fall 2014 session of classes begins Monday, September 15.
Chicago Human Rhythm Project's Free 4 All takes place Monday-Saturday, September 8-13 at the American Rhythm Center at the Fine Arts Building, 410 S. Michigan Ave., 3rd floor. For information or an updated class schedule, visit arcchicago.org or call 312-922-1272.
About Author
- Casting Roundup: Sarah Chalke, Kim Raver Headline New Pilots
- ADVENTURE TIME Headed to the Big Screen?
- Pilot Season Roundup: Colm Feore, Daniel Stern, Dave Annable to Star in New Shows
- James Franco to Star in New Fox Comedy, WHY HIM?
- Christopher Meloni to Star in WGN America's Slave Drama UNDERGROUND
- Check Out Alex Dunphy's Full College Essay from Latest MODERN FAMILY Episode
- Photo Flash: HBO Reveals Key Art for GAME OF THRONES Season 5
- Syfy to Honor Leonard Nimoy with Special Programming This Sunday
- Grammy Winner Kelly Clarkson Exclusively Premieres New Music from New Album on MTV, VH1 & CMT First
- CONSTELLATIONS' Jake Gyllenhaal and Ruth Wilson Set for THEATER TALK This Weekend


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Game of Thrones Season 5 Marks the Point When the Adaptation Debate Consumed the Show
Even GRRM is sick of it.By Nate Jones
Even GRRM is sick of it.By Nate Jones
"If you portray a utopia, then you probably wrote a pretty boring book."By Nate Jones
He says they still have a part to play in his books.By Nate Jones
"When I read that scene, I kinda loved it."By Nate Jones
George R.R. Martin says he hopes to be finished in 2016.By Nate Jones
He says he's in the home stretch.By Nate Jones
George R.R. Martin will have a rough draft for his ending.By Nate Jones
They may compromise by splitting the final season in two.By Nate Jones
So much enthusiasm for one pen.By Nate Jones
"People are going to die who don't die in the books."By Nate Jones
So many love triangles!By Nate Jones
She'd play a witch in In the Lost Lands.By Nate Jones
According to George R.R. Martin's publisher.By Nate Jones
Maybe.By Jennifer Vineyard
The original Dance With Dragons manuscript contains handwritten notes between Martin and his editor.By Nate Jones
We always knew he was Santa.By Nate Jones
The novelist on Downton Abbey, sex, and death.By Jennifer Vineyard
Why he wrote this companion guide instead of The Winds of Winter.By Jennifer Vineyard
The grave-hopping, pizza-scarfing Game of Thrones author also thinks we should free the nipple.By Jennifer Vineyard
Which of his dragons could beat Smaug?By Nate Jones


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While we know how much insurers and oil and gas companies dole out to political campaigns and lobbyists, we don't have a clue how much of their cash is used to establish front groups or how much of it winds up in the pockets of either pundits for hire or tax-exempt organizations that do their bidding.
Although the employment situation for veterans has improved since I left the military, the most recent report from the Bureau of Labor Statistics tells me we still have work to do. Every man and woman who has honorably worn the uniform of this country has the ability to make our nation stronger. I know because they already have.
We all remember the childhood story of Pinocchio and the valuable life lesson it taught us about the importance of telling the truth. I've repeated the same story to my own children in hopes that a growing nose may dissuade them from lying. Unfortunately, the Pinocchio story doesn't seem to have impacted the U.S. Chamber of Commerce or Crossroads GPS.


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The Essential Tool Chest
Workbench Tool Storage Solutions
Drawbore Your Mortise-and-Tenon Joinery
A Woodworker's Guide to Grain Direction
Simple Cabinetry with Pocket Hole Joinery
Capture More Dust from Your Router Table
How to Sharpen a Spokeshave
Hinge Mortises on the Tablesaw
How to Sharpen Hollow Chisel Mortising Bits
The Coolest Cutting Board Ever?
Finishing Technique for Greene and Greene Furniture
Simple Tape Trick for Tight Fitting Through-Mortises
Smoothing Plane Tips and Techniques
Customize Your Router for Centered Mortises
Speed Up Handplane Honing with Your Ruler
Coffee Tablecomments (4) December 28th, 2009 in Reader's Gallery
My wife asked me to make her a Coffee Table for Christmas. I thought that this would be a nice easy project to spend some time on, so I asked her if she had any ideas about the design. She said in fact she had seen a "beautiful table" in a magazine and produced a picture of Paul Schurch's Ribbon Table that she had found in one of my design magazines from Fine Woodworking. I had to explain that I really didn't have the expertise to produce something of that nature as I had never veneered before and had only very limited experience at bent laminations. But, my wife asks for so little I thought that I would give it a try and so 172 pieces and 2 1/2 months of my spare time later it was finally finished and with 2 days to spare!
Design or Plan used: My own design
posted in: Reader's Gallery, table, modern, walnut, veneer, inlay, bent lamination, holly, birdseye maple, walnut burl veneer
Comments (4)
Nice job Eric
And remember - happy wife, happy life.
Posted: 11:11 am on December 21st
Posted: 12:18 pm on January 12th
Posted: 5:31 pm on December 28th
Jason M.
Posted: 10:09 am on December 28th
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More Information:
|100% Cotton
|Black, Gray, Ivory
|Wash care:
|Hand Wash Separately
|Catalog No.:
One of my favourite pieces since it is really nice to wear but a little to thin for the winter months
I was actually surprised at the quality of this shirt, although I agree with another reviewer that the material didn't feel like 100% cotton, more like a blend. Regardless, I think it would hold up really well over time. Unfortunately it was just too large for my frame (5'1"/155cm) to be flattering. I've got a number of large drapey shirts and sweaters from Korea that work for me just fine, but this looked like a sack. I had to return it.
Not the best quality top, and slightly overpriced... but simple and cute nonetheless!
It was thinner than I expected, but all in all good purchase.
it's a really nice top, i was expecting it would be a bit more loose fitted like on the model, but thats probably because the model is thinner than i am.
overall I really like the look and it still looks good even if it's not a baggy as I wanted.
This is a good product, good material and quality. It is comfortable to wear.
It is nice quality, but doesn't quite fit me the same -- I'm on the thin side, but I have a medium build. If I tuck it into pants/skirts it looks good. All depends on how you wear it! Probably better to go with something else considering the price for what it is.
Very comfortable and fits me perfectly, I love the neckline and the way it fits me. Yes, a little bit overpriced but a great basic piece for any wardrobe.
It's baggy but I wish it was made of a thicker material. The quality does not match the price.
I doubt the material is actually 100% cotton...probably more like polyester.
DANI LOVE keeps young, active women in mind with pants, denims, tops and outerwear that look and feel comfortable. Founded in 2006 by designer Lee Bang Hee, the brand adheres to a simple aesthetic that shows off the female form without sacrificing a sense of ease or modesty. YesStyle is an authorized retailer of DANI LOVE.


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« April 10 April 12 » Friday, April 11, 1997
You Can't Judge A Blues Pianist By His Age Or Baby Face
April 11, 1997 in Seven A.J. Croce, son of Jim Croce, makes his first trip to Idaho this weekend for three sold-out performances.
Offbeat Universe Of Coen Films Halfway Between Real, Surreal
April 11, 1997 in Seven Thugs Carl Showalter (Steve Buscemi) and Gaear Grimsrud (Peter Stormare) await their contact in "Fargo."
Big, Bad Snake Gives Good-Time Fright
April 11, 1997 in Seven Jon Voight has a good time portraying a not-too-seriuos snake poacher in "Anaconda."
Killer Role For Cusack 'Grosse Pointe Blank' A Hit-Man Story Without The Sadistic, Nonchalant Violence
April 11, 1997 in Seven John Cusack's character is a hit man who travels back to his hometown on a job, and hooks up with a former girlfriend.
'Magic Flute' Blends Genius Of Two Artists
April 11, 1997 in Seven "The Magic Flute" is being shown as a benefit for KPBX.
Brash Bette Shines In 'That Old Feeling'
April 11, 1997 in Seven (Scene from film)
Zephyr Serves Up Mexican Offerings
April 11, 1997 in Seven The musicians of Zephyr will provide a hot evening of chamber music tonight with percussion music by Mexican composer Carlos Chavez and "The Grand Tango" by Argentina's greatest modern master …
Big Band Sound Coming To Hobart's
April 11, 1997 in Seven Saturday night at Hobart's, Tom Molter's All-Star Big Band, with Gary Gemberling on vocals, will recreate one of the golden eras of American music. Molter is a trombone player and …
Europa Will Sweeten Up Tax-Filing Day
April 11, 1997 in Seven A spoonful of sugar helps the medicine go down, right? Europa wants to help soothe the sting of tax day with a dessert extravaganza. For $10, guests will be invited …
Lead Expert Says River Likely Toxic Health Care Professionals Warned To Prepare For Lead-Poisoning Cases In Washington State
April 11, 1997 in Nation/World on Page A1 Rosen
U.S. Judge Rules Line-Item Veto Unconstitutional Law Usurps Authority Of Congress
April 11, 1997 in Nation/World on Page A1 One year after enactment of a law giving the president line-item veto power over congressional spending bills, a federal judge ruled Thursday that the law is unconstitutional. The line-item veto …
Spokane River Probably Toxic, Says Expert Doctors Advised To Count On Cases Of Lead Poisoning
April 11, 1997 in Nation/World on Page A1 Rosen
They Love Those Swingin' Sounds Music From Another Era Echoes At Hobart's
April 11, 1997 in Nation/World on Page A1 Jazzy combo Mona Clark and Hal Poffenroth perform at Hobart's Jazz Lounge. Photo by Colin Mulvany/The Spokesman-Review
Batt Denounces Plan To List Bull Trout As Endangered Governor Says Fish And Wildlife Proposal Ignores Idaho Efforts To Save Species
April 11, 1997 in Nation/World on Page A1 Gov. Phil Batt accused the Clinton administration on Thursday of sabotaging his efforts to keep the bull trout off the endangered species list. Citing dozens of Idaho projects to benefit …
Commission Erred On River, Creek Buffer Hearings Board Also Says Penalties Must Be Part Of Growth Ordinance
April 11, 1997 in Nation/World on Page A1 FROM FOR THE RECORD (Saturday, April 12, 1997): Correction Clarifying who's who: The Washington Environmental Council joined a Spokane environmentalist in challenging Spokane County's "critical areas" ordinance before the Eastern …
Developer Has Designs On Post Falls 1,613 Homes Expected To Strain Services
April 11, 1997 in Nation/World on Page A1 Montrose Community would fill this field next to the Prairie Ridge subdivision in Post Falls. Photo by Jesse Tinsley/The Spokesman-Review
Campaign Financing A Real Snooze
April 11, 1997 in Nation/World on Page A2 Lincoln bedroom guests may be the only Americans who've lost sleep so far. File/Associated Press
Lottery Numbers
April 11, 1997 in Nation/World on Page A2 Wednesday's Washington Triple Choice: 629. Wednesday's Washington Keno: 7-12-13-16-19-20-27-29-30-32-37-44-45-53 -56-61- 72-75-79-80.
April 11, 1997 in Nation/World on Page A2 Ten years ago: Invoking emergency powers, the government of South Africa outlawed any action, word or written document protesting the practice of detention without trials or calling for the release …
Today In History
April 11, 1997 in Nation/World on Page A2 In 1789: A group of Baltimore workers asked Congress for tariff protection. The workers said that imports were hurting American workers. In 1814: Napoleon abdicated and was banished to the …
Online Library To Enrich Offerings
April 11, 1997 in Nation/World on Page A3 The nation's library on the Internet will acquire 19th-century sheet music by black Americans, old photographs from the Great Plains and other historical materials by tapping into several libraries across …
Government To Hire 10,000 Off Welfare President Hopes Employers Across Nation Will Follow Suit
April 11, 1997 in Nation/World on Page A3 In his latest effort to make last year's sweeping welfare-reform law work, President Clinton announced Thursday that the federal government will hire 10,000 people from welfare rolls during the next …
Kidnap Pulls Town Together Porch Lights Burn, Polly Klaas Searchers Join Effort To Find Boy
April 11, 1997 in Nation/World on Page A3 Anthony Michael Martinez, 10, was kidnapped at knifepoint last Friday. Photo by Associated Press
Flood Victims Argue For Eased Species Act
April 11, 1997 in Nation/World on Page A6 Members of Congress and California landowners called Thursday for relaxation of the Endangered Species Act, saying it was responsible for millions of dollars of property damage in last winter's devastating …


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Marijuana Blog
Colorado issues first medical-marijuana business licenses in U.S.
Category: Medical Marijuana | Posted on Thu, October, 27th 2011 by THCFinder
Colorado is setting the bar to support Dispensaries and Medical Marijuana Patients by giving out medical marijuana business licenses to local dispensaries. Hopefully other states will start following what Colorado has done to show the support and hopefully get the federal government to back down on their raids.
Colorado has begun issuing the first state medical-marijuana business licenses in the nation, the culmination of a more than year-long application process for dispensaries and marijuana- infused-products makers.
The state has issued 11 licenses to businesses in Boulder, Denver, Colorado Springs, Fort Collins and Littleton, according to the state Medical Marijuana Enforcement Division of the Department of Revenue. Another seven have been notified they are likely to receive a license. And the state has sent out letters to local governments for 467 dispensaries and products-makers to double-check that those businesses have local approval — one of the final steps in the licensing process.
The first license to a Denver business was presented Wednesday to Dr. J's owner Tom Sterlacci at a meeting of an industry workgroup. The presentation received a standing ovation.
"It's very historic," Sterlacci said. "Now we're not standing alone with the feds. We have the city and the state standing with us."
Medical-marijuana advocates say Colorado's regulations for cannabis businesses are the most comprehensive in the nation, and they credit the rules' thoroughness with shielding the businesses from federal raids. John Ingold, The Denver Post
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Participate in This Event or find another event
Friday, April 10, 2015
Johns Hopkins University
3400 N Charles St, Baltimore, MD Map it
Already a participant?
Log in here
43 teams and 430 participants have raised $39,270.47
Total above may not reflect all participants, teams, and donations
Relay is just a few days away!!
Rain or Shine!
Online Registration and Online Luminaria Sales will close
at 12 noon on Thursday, April 9th
You can still register at the event for $15
Luminaria will be avaliable for a donation at the event.
See you there!!
American Cancer Society Relay For Life campus events serve as a powerful way for you to raise awareness and funds in the fight against cancer, from helping people facing the disease in your community to ensuring cancer is a thing of the past for future generations. The Relay For Life movement features campus and community events that offer an opportunity to honor the cancer survivors in your life, promote how you can reduce your cancer risk, and raise money to help find a cure. Today, with the support of thousands of student volunteers, the American Cancer Society is helping save more than 400 lives a day. And we won't stop until we end cancer once and for all!
Remember or honor a loved one who has battled cancer with a luminaria tribute bag >>
Cancer survivors and caregivers find a community of hope and support >>
Find out more details about your campus Relay For Life event >>
Contact Taylor Lutz:
(410) 931-6850
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Book Accommodation online in Longtown
Bessiestown Farm Country Guesthouse, Carlisle
This multi award winning, 5 Star Gold, Country Guest House is situated in a magnificent rural setting in the heart of the Debatable Lands, just north of Carlisle, midway between ... More Prices from £95.00
Lynebank House, Carlisle
Lynebank House is situated amidst the rolling countryside of Cumbria in the rural hamlet of Westlinton, a 6 mile drive from Carlisle & the M6 on the busy A7 tourist ... More Prices from £65.00
Graham Arms Hotel, Longtown
The Graham Arms is a former Coaching Inn and was built about 200 years ago. In days gone by, the hotel was an overnight coaching stop; the horses would be ... More Prices from £75.00
Greens at Gretna, GRETNA
Formerly Garden House Hotel, Greens at Gretna is currently under refurbishment. Once completed (by summer 2015) the hotel will be modern and stylish, providing a quality 4-star experience at 3-star ... More Prices from £60.00
Anvil View Guest House, Gretna
Luxury Bed and Breakfast accommodation situated in the heart of romantic Gretna Green ... The Anvil View Guest House is two minutes walking distance from the World Famous Blacksmiths Shop ... More Prices from £75.00
Smiths at Gretna Green Hotel, Gretna Green
The new, exciting Smiths at Gretna Green embraces both tradition and modern day comfort with inspirational design set against a historical backdrop. Guests can enjoy a blend of professional, relaxed ... More Prices from £60.00
Surrone House, Gretna
Surrone House, a former farmhouse, has been in the same family for the past 36years.The house has been carefully converted to provide six comfortable appointed rooms, all with en-suite facilities, ... More Prices from £70.00
Gretna Hall Hotel, Gretna Green
Originally built in 1710 as a manor house, our hotel; set in 10 acres of beautiful gardens offers all the character of a substantial historical building with all the facilities ... More Prices from £81.00
Hunters Lodge Hotel, Gretna
The proprietors personally supervise the smooth running of the hotel (a modernised listed building) which is situated in the heart of Gretna and is also the nearest hotel to the ... More Prices from £58.00
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Facebook Ruins Birthdays, Christmas, and Harry Potter
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Sad and embarrassing moments on Facebook
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The state of the music industry
Another one got caught today, it's all over the papers. "Drug Bust" Damn kids. They're all alike. But did you, in your three-piece psychology and 1950's technobrain, ever take a look behind the eyes of the smokers? Did you ever wonder what made the...
Rap music's defining feature is not the cursing, the bravado, or even the beefs -- it's the genre's
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Rumor has it on the internet that .
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Human Traffic Very unique comedy about the drug/club culture in the UK. Five friends ponder society, drug use and their own lives as they go about their usual weekend of snorting, smoking, popping, dancing and sex. The Matador Pierce Brosnan plays an...
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Xbox, Kindle Fire, Roku, Smart TV—no matter what device you have, you can find Crackle on it. Each month, 18 million users in the U.S. access the Sony-owned, advertiser-supported streaming network to watch a selection of movies and TV shows, as well as a growing number of original series, including Jerry Seinfeld's Comedians in Cars Getting Coffee. But Crackle still has a bit of an identity crisis as it looks to make a name for itself among the likes of Netflix and Amazon. After an erroneous New York Post report in December indicated that Sony—at the time under siege from hackers and seeking alternative distribution for its controversial comedy The Interview—would be releasing the film for free on Crackle, most people said, "What's Crackle?" (In the end, Crackle landed rights to the film next year in the first ad-supported window.) Crackle's general manager and evp Eric Berger, who's also general manager of digital for Sony Pictures Television, is trying to change that. He's taking big swings like commissioning Joe Dirt 2: Beautiful Loser—the first digital sequel to a movie—and pushing original series like the drama Cleaners, starring David Arquette and Gina Gershon, and the thriller Chosen, starring Chad Michael Murray and Rose McGowan. And in his loudest statement yet, he's moved Crackle out of the NewFronts and into the upfronts for the first time this year: on April 14, it will become the first streaming network to present at the upfronts. On the eve of Crackle's first upfront, Berger talked about his bold move, what really happened with The Interview and meeting the challenge of raising Crackle's profile.Read More
The seeds of Yahoo's St. Patrick's Day premiere of season six of "Community" were planted when CEO Marissa Mayer made a critical decision in January 2014 about the future of the Internet media company's video biz. Mayer, on the job for 18 months after leaving Google, had just fired chief operating officer Henrique de Castro, whose... Read moreRead More
In a heated exchange before a judge, YouTuber Commander Holly (real name: Holly Conrad) faces off against actress Tina Huang. The issue at hand is one that has divided many a Trekkie: William Riker, yay or nay? Welcome to Nerd Court, where arguments that one might normally witness at a comic book store instead are heard in a "court of law,"Read More
After releasing two new "Cinderella" trailers to ring in the new year, Disney has debuted a featurette via Yahoo Movies. Aside from new footage from the film, the clip also features interviews with director Kenneth Branagh and stars Lily James (Cinderella), Cate Blanchett (Cinderella's stepmother), "Game of Thrones" alum Richard Madden (Prince Charming) and Helena... Read moreRead More
It's the end of the year, and Tumblr is recapping its top branded entertainment posts of 2014. With the average desktop user spending 14 minutes per visit in October, according to comScore, it's clear why leading entertainment companies are advertising on the Yahoo-owned microblogging site.Read More
It's the end of the year, and Tumblr is recapping its top branded entertainment posts of 2014. With the average desktop user spending 14 minutes per visit in October, according to comScore, it's clear why leading entertainment companies are advertising on the Yahoo-owned microblogging site.Read More
Yahoo announced a deal to acquire BrightRoll, a programmatic video-advertising platform, for $640 million in cash. The deal will combine Yahoo's desktop and mobile video advertising inventory with BrightRoll's platform and publisher relationships. BrightRoll's net revenue is expected to exceed $100 million this year, and Yahoo said it expects the transaction to enhance its profitability.... Read moreRead More
Entertainment companies have yet another social-marketing tool to play with: Yahoo's Tumblr micro-blogging site has launched autoplay video ads, with initial advertisers including Universal, CW and Hulu. The video ads on Tumblr will show up in users' feeds, muted by default and presented in a continuous loop — like Facebook's autoplay ads. On Tumblr's Android and iOS apps, the... Read moreRead More
The stars of the cult TV classic "Mystery Science Theater 3000″ are having a reunion of sorts. Joel Hodgson and Trace Beaulieu have joined the cast of "Other Space," a half-hour comedy created by Paul Feig ("Bridesmaids") for Yahoo. The series just began production in anticipation of a launch in the spring of next year.... Read moreRead More
Shareholder activist urges merger talks between online giantsRead More


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Network After Work Detroit at The Corner
- Business & Professional
- 100 Townsend St, Birmingham, MI, 48009
Network After Work Philadelphia at Ladder 15 ***Sold Out***
Network After Work
Wednesday, February 6, 2013 from 6:00 PM to 9:00 PM (EST)
Join Network After Work on Wednesday February 6th at (1528 Sansom St.) from 6-9pm. Events are for professionals of all industries and career levels interested in expanding their professional network and take place monthly which allow guests a chance to foster new professional connections in a relaxed atmosphere conducive to business and social networking. Upon entering, guests will receive a name tag color coded by industry which allows for easy navigation. Network After Work events allow guests a chance to get their name and brand in front of some of Philadelphia's top business professionals while visiting the city's best After Work destinations.
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text: Friday, February 22, 2013 - Seductive nose showing plenty of toasted oak aromas, followed by tons of fresh lemon juice, some soapy floral notes, orange zest, and some chalky limestone. More charred oak on the palate intertwined with fresh lemon juice, tart pineapple, honey, melon, white flowers, and a hint of herbal notes. Plenty of acidity, with a long length finish ending on notes of white flowers and lemon. This was a pleasant surprise, fairly ripe and round on the palate yet carrying the acidity to bring everything into perfect balance. Layered, complex, with a terrific viscous mouth feel. Drinking beautifully at the moment with no signs of slowing down. Fantastic.Post a Comment / View MattTM's profile


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Some would say there aren't many reasons to make the trek from Boston to Cape Cod, but James Boden has one that's close to his heart: Futsal.
Though winter is just beginning on Cape Cod, fans of the Cape Cod Baseball League already have their calendars marked for the start of the 2013 season.
The ice on the Butler Rink of the Hyannis Youth and Community Center was the place to be Dec. 13, especially for those eager to skate with the Barnstable boys ice hockey team.
When a team wins a game, it's always exciting. It's even sweeter when everyone on the team has a chance to play, and sweeter still when every player scores.
Click HERE to subscribe to e-mail updates from barnstablepatriot.com.
CONTACT US | The Barnstable Patriot | 4 Ocean Street | P.O. Box 1208 | Hyannis, MA 02601 | 508-771-1427 | Copyright © 2015 Local Media Group, All Rights Reserved.


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World Bank Approves $188 Million for Rehabilitation of Guddu Barrage
Washington DC, June 19, 2015 ─ The World Bank today approved US$188 million to improve the reliability and safety of the Guddu Barrage and strengthen the Sindh Irrigation Department's capacity to oper... Show More +
Pacific Islands Forum Secretariat - Pacific Resilience Program
IDA Grant: US$1.32 million equivalentGEF Grant - Special Climate Change Fund: US$0.90Project ID: P155542Project Description: The objective of the project is to strengthen early warning, resilient inve... Show More +
Pacific Islands – Secretariat of the Pacific Community - Pacific Resilience Program
IDA Grant: US$3.68 million equivalentPilot Program for climate Resilience Grant: US$5.79 million equivalentProject ID: P147839Project Description: The objective of the project is to strengthen early w... Show More +
Republic of Vanuatu - Pacific Resilience Program
IDA Credit: US$1.50 million equivalentMaturity = 40 years, Grace = 10 yearsProject ID: P155256Project Description: The objective of the project is to strengthen the financial protection of Vanuatu.
Republic of the Marshall Islands - Pacific Resilience Program
IDA Grant: US$1.50 million equivalentProject ID: P155257Project Description: The objective of the project is to strengthen the financial protection of the Republic of the Marshall Islands.
Kingdom of Tonga - Pacific Resilience Program
IDA Credit: US$6.00 million equivalentMaturity = 40 years, Grace = 10 yearsIDA Grant: US$4.50 million equivalentGlobal Facility for Disaster Reduction and Recovery Grant: US$1.50 million equivalentGEF... Show More +
Independent State of Samoa - Pacific Resilience Program
IDA Grant: US$13.79 million equivalentProject ID: P154839Project Description: The objective of the project is to strengthen early warning, resilient investments and financial protection of Samoa.
China's New Approach to Flood and Watershed Management
Participatory Watershed ManagementIn Xining, like many other places in China, watersheds face environmental problems, including soil erosion, landslides, heavy sedimentation, water pollution, floods a... Show More +
Pacific Islands Prepare for a More Disaster Resilient Future
WASHINGTON, June 19, 2015 – The World Bank's Board of Executive Directors today approved US$32.29 million in International Development Association (IDA) grants and credits to support the Pacific Resil... Show More +
Cambodia: Consultation Plan
Key Stakeholder Groups to Be ConsultedThe WBG will hold several consultation meetings with different stakeholder groups. Participants in the consultation with civil society organizations (CSO) will be... Show More +
Independent State of Samoa - Pacific Regional Connectivity Program - Phase 3: Samoa Connectivity Project
IDA Grant: US$16.0 million equivalentProject ID: P128904Project Description: The objective of the project is to reduce the cost and increase the availability of Internet services in Samoa.
Underwater Cable to Bring High-Speed Internet to Samoa
Washington D.C., June 19, 2015 – Samoans will soon be connected to cheaper and higher-capacity internet access after the World Bank's Board of Executive Directors approved a grant of US$16 Million for... Show More +
Powering Guinea: The World Bank Group Supports Improved Energy Supply and Electricity Services
CONAKRY, June 19, 2015—The Government of Guinea is signing a management contract today with the Veolia Africa-Seureca consortium, which combines two experienced utilities management firms, to enhance ... Show More +
Sindh Barrages Improvement Project (SBIP)
WASHINGTON, June 19, 2015 - The World Bank's Board of Executive Directors today approved the following projectPakistan – Sindh Barrages Improvement Project (SBIP)IDA Credit: US$188 million equivalentT... Show More +
World Bank Grants $55 million for Palestinian Development
Washington, June 19, 2015 – The World Bank Board of Governors authorized the transfer of US$55 million to replenish the Trust Fund for Gaza and West Bank from which the World Bank has, since 1993, pro... Show More +
Beyond Connections: Energy Access Redefined
Is having an electricity line run into your home the same as you being able to turn on lights or appliances without worrying if it is affordable and of good quality? And is access to energy any good i... Show More +
Asian Countries Are among Top Achievers on Sustainable Energy Progress
Manila – June 18, 2015 - Asian countries are making a vital contribution to achieving global sustainable energy goals, a new World Bank report finds. But while the region performs strongly on ensuring... Show More +
Mongolia's Ministry of Finance and World Bank sign credit agreements for projects supporting education quality and transparent and efficient service delivery
ULAANBAATAR, June 18, 2015 — Minister of Finance J. Erdenebat and World Bank Country Manager James Anderson signed today credit agreements totaling US$63 million for three new projects: the... Show More +
New World Bank Report Looks at How to Bring On-site Sanitation to Ger Areas of Ulaanbaatar
Ulaanbaatar, June 18, 2015 — Representatives from municipality, districts and government ministries, as well as CSOs and development organizations gathered today to participate in a Roundtable discuss... Show More +
Corporate Governance and Financial Reporting Global Solutions Groups
OVERVIEWCorporate governance and financial reporting (CGFR) are key building blocks of a well-functioning market economy. Sound reporting, controls, auditing and corporate governance reduce the risks ... Show More +


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The Malik Report
Red Wings captain Henrik Zetterberg speaks with WJR's Mitch Albom
by George Malik on 05/21/14 at 02:39 PM ET
Comments (1)
Henrik Zetterberg appeared on WJR's Mitch Albom show on Tuesday night, discussing the Henrik Zetterberg Foundation (which raised $8,800 for Detroit schools), the establishment of a charitable initiative called "Club 40" (and its partnership with Albom's charity, SAY Detroit), his recovery from back surgery and the Wings' short playoff run. Zetterberg spoke with Albom for 19 minutes, and he was very glib and comfortable (the cliche machine was dialed back significantly). Enjoy:
(Soundcloud is having some issues, so if you can't see the player, the download link is here).
Filed in: | The Malik Report | Permalink
Red Wings mid-day news: Griffins locker room clean-out-day Tweets and 'Mantha vs. Bertuzzi'
by George Malik on 05/21/14 at 02:21 PM ET
Comments (48)
Amongst this afternoon's Red Wings-related news stories:
The Grand Rapids Griffins' locker room clean-out day wasn't exactly covered by oodles of media outlets, but Fox 17's Steve Amorose still snagged some particularly interesting tidbits regarding coach Jeff Blashill's future, the Wings' back-up situation and Mitch Callahan's desire to remain a member of the organization even if he has to return to the AHL:
Stopped by Griffins locker clean out today. Key takeaways: 1) Blashill plans on being back in GR next year, emphasized his contract status
2) Petr Mrazek was as diplomatic as can be, but says he feels he has nothing left to prove in AHL and is ready for NHL challenge.
3) Mitch Callahan is an RFA. About making Red Wings next year, he called it an outside chance.
3 cont.,) Callahan says he'd be happy to return to GR and continue to develop as a player. He also got wires removed yesterday from mouth.
(I don't think the Wings will have a hard time agreeing with Mrazek)
In another prospect-related vein, the Red Wings posted a seven-minute video chronicling Val-d'Or Foreurs forward Anthony Mantha and Guelph Storm forward Tyler Bertuzzi's Memorial Cup experiences-in-progress (Guelph plays London tonight at 7 PM EDT, and the game will air on Sportsnet and the NHL Network):
Filed in: | The Malik Report | Permalink
Former Wing Brian Rafalski discusses the pratfalls of a professional athlete's lifestyle
by George Malik on 05/21/14 at 04:35 AM ET
Comments (1)
When Brian Rafalski retired from the Detroit Red Wings in 2011, leaving $6 million in salary on the table, he stated that a religious calling played a large role in his decision-making process. Rafalski made a short comeback with the ECHL's Florida Everblades this past season.
The Fort Myers (Florida) News-Press's Craig Handel reports that Rafalski, who now coaches youth hockey near Naples, Florida, shared some of the reasons that he's hung up his skates for good while speaking at the News-Press's All-Stars Banquet:
"Much of what marketing and media show fans is exclusively the good," the Sanibel resident said. "Much of the bad is glossed over and ignored. Image takes precedence. Then we're shocked when athletes make mistakes in their public and professional lives."
Rafalski starred in the NHL despite being a 5-foot-10 defenseman who didn't make the pros until age 26. He had to play a year in Sweden.
However, those weren't his biggest obstacles or trials and tribulations. Without going into specifics, Rafalski talked about being the prodigal son who drifted and indulged in things he should have avoided.
"I failed as a husband and father," he said. "If I continued on this course, I'd lose my family."
In sharing why he'd admit his mistakes in front of a room full of strangers, he said, "That's life – if we're honest with ourselves. It's not through joy I do that. It's very humbling. It's not something I enjoy but it's something that's part of my life and will affect how my life will be in the future."
Handel continues, and wherever Rafalski's calling takes him, I hope he's found a happier and more fulfilling path.
Filed in: | The Malik Report | Permalink
Red Wings overnight report: programming notes and Twitter stuff; on Howard, Mantha and Ferraro
by George Malik on 05/21/14 at 04:14 AM ET
Comments (1)
I've been spending most of my time recapping Memorial Cup games and World Championship games, so this overnight report's a little light, but it's not due to a lack of effort on the Foreurs-Oil Kings recap or any of the Worlds news.
As Paul posted on Tuesday evening, the World Championships' quarterfinals schedule was finalized after Tomas Tatar's Slovaks bowed out of the tournament, Justin Abdelkader scored 2 goals for the U.S. and (I missed this one) Jakub Kindl had an assist in the Czechs' 5-4 OT win over France.
On Thursday, Abdelkader's and Danny DeKeyser's Americans will play Kindl's Czechs at 9 AM EDT (USA Hockey's website reports that NBCSN will air the game on tape delay at 9:30 AM).
The winner of the U.S.-CZE game will play the winner of Thursday's Canada-Finland game in Saturday's semifinal 1, which takes place at 7:45AM;
Also on Thursday, Gustav Nyquist's Swedes will play Belarus at 2 PM (and yes, the Swedish press is already freaking out thanks to flashbacks of Belarus beating Sweden at the 2002 Olympics).
Filed in: | The Malik Report | Permalink
Red Wings playoff prospect news: Mantha's Foreurs take 2-OT decision at Memorial Cup
by George Malik on 05/21/14 at 12:19 AM ET
Comments (0)
Updated substantially at 2:28 AM: Anthony Mantha had only an assist as his Val-d'Or Foreurs defeated the Edmonton Oil Kings 4-3 in a double-overtime decision at the Memorial Cup, but his linemate, Anthony Richard, scored the game-winner, and the fact that Mantha's getting the snot checked out of him by new opponents is essential for his growth as a player (he has 1 goal, 2 assists and is a -1 in 3 games).
The win assures that the Foreurs won't have to play Thursday's tiebreaker--instead, they'll await the winner of Thursday's Edmonton-London tilt, and whichever team's playing back-to-backs will battle Val-d'Or on Friday, and whichever team wins Friday's game will advance to play Tyler Bertuzzi's Guelph Storm in Sunday's championship game.
Guelph will wrap up its round-robin play against the London Knights on Wednesday evening,
but that game doesn't really count for much of anything as London will have to play in Thursay's tiebreaker--and it turns out that the game means something as a London win = London plays Edmonton on Thursday, and a London loss = the hosts are eliminated.
However, Guelph will get three days off to wait for the winner of Friday's game no matter what happens.
First and foremost, the Oilers' website posted a superb highlight clip:
Filed in: | The Malik Report | Permalink
IIHF World Championship Quarter Finals Schedule
by Paul on 05/20/14 at 05:11 PM ET
Comments (29)
George asked me to post the starting times for the IIHF World Championship Quarter Finals Games.
The games are this Thursday, May 22nd and listed in ET time.
via the IIHF,
Quarter-Finals on 22 May 2014
At Minsk Arena:
Russia vs. France 10:00am ET
Sweden vs. Belarus 2:00pm ET
At Chizhovka Arena:
USA vs. Czech Republic 9:00am ET
Canada vs. Finland 1:00pm ET
Filed in: | The Malik Report | Permalink
A programming note: Zetterberg to appear on WJR this evening
by George Malik on 05/20/14 at 12:34 PM ET
Comments (0)
The Henrik Zetterberg foundation recently attempted to donate $10,000 to Detroit schools via a Twitter campaign, and they got 88% ($8,800) of the way toward their goal. As such...
Filed in: | The Malik Report | Permalink
Red Wings at the Worlds: Tomas Tatar scores 2 as the Slovaks bow out
by George Malik on 05/20/14 at 12:29 PM ET
Comments (1)
After Justin Abdelakder scored 2 goals in the Americans' win over Germany, Tomas Tatar matched Abdelkader's total, scoring the 1-0 and 4-2 goals en route to Slovakia's 4-3 win over Denmark (today's remaining Wings activity: Jakub Kindl's Czechs vs. France @ 1:45 PM EDT, and Anthony Mantha's Val-d'Or Foreurs vs. Edmonton @ 7 PM, on the NHL Network and Sportsnet)...
But the game was painful to watch at times, because neither Slovakia and Denmark made the World Championship's quarterfinals, and as such, the teams didn't "engage" until the last minute of the 3rd period.
For the Slovaks, losing their quarterfinal spot to France is a particularly stinging loss, and instead of playing for a medal, Tatar, whose most exuberant celebration involved a mid-level fist pump on his second goal, his Medal Round activities most likely include heading back to Metro Detroit to pack up his apartment before taking a few weeks off (back to Sunny Beach in Bulgaria?) before engaging in off-season training in Trencin, Slovakia.
Filed in: | The Malik Report | Permalink
Wallner: Grand Rapids Griffins roster's shape entinwed with Wings personnel moves
by George Malik on 05/20/14 at 11:56 AM ET
Comments (15)
Based upon yesterday's Tweets and Instagram pictures, the Grand Rapids Griffins chose to remain in Austin after the Texas Stars eliminated them from the Calder Cup playoffs on Sunday evening, enjoying one last day as teammates in the warm weather before returning to Grand Rapids to clean out their lockers and engage in the same kinds of exit interviews that the Wings hold after their season ends.
The Grand Rapids Press's Peter J. Wallner notes that many of the Griffins' moving parts and summertime decisions (Ryan Martin is now the Griffins' GM) will depend upon the Wings' personnel decisions, and as you already know, that's true about the team's decision whether to bring back Jonas Gustavsson or to promote Petr Mrazek...
Filed in: | The Malik Report | Permalink
'Red Army's' director was drawn in by Russian hockey's stylistic flourishes—like many Wings fans
by George Malik on 05/20/14 at 10:57 AM ET
Comments (1)
I get the feeling that yesterday's pair of entries regarding the surprising popularly of Red Army are the tip of the iceberg. This film's going to make quite the impact--Sony Pictures Classics doesn't snag a documenary's rights prior to the Cannes Film Festival unless it's good.
As someone rasied on the puck possession hockey of the Russian 5, I can only smile while reading director Gabe Polsky tell the Associated Press that he was attracted to the subject matter because of the way the Russian national team played hockey (you will still hear many "new" Wings fans state that they were attracted to the team because the system the Wings have played for the past 20 years is exciting and/or pleasant to watch).
The movie explores the social and cultural changes in the Soviet Union, then Russia, through the experiences of the national hockey team, and the life of its superstar captain, Fetisov.
Polsky's first impressions of the "Red Army" team were their balletic style of play, compared to the more brutish style practiced in the United States.
"You, know I preferred this style of play and I think it really evolved sport and hockey to another level," said the director in an interview here last week. "That really piqued my interest about the Soviet Union and my roots, through hockey, and I wanted to explore why, how they got so good and what was going on over there."
The interview continues, and again, Fetisov is anything but a present-day critic of the Putin regime:
Filed in: | The Malik Report | Permalink
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About The Malik Report
The Malik Report is a destination for all things Red Wings-related. I offer biased, perhaps unprofessional-at-times and verbose coverage of my favorite team, their prospects and developmental affiliates. I've joined the Kukla's Korner family with five years of blogging under my belt, and I hope you'll find almost everything you need to follow your Red Wings at a place where all opinions are created equal and we're all friends, talking about hockey and the team we love to follow.
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text:
How fans are called?
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November 20 2012, 12:55 AM
Mr. Laser Beam
Fleet Admiral
Location: The visitor's bullpen
Re: How fans are called?
Andrew_Kearley wrote:
Robert D. Robot wrote:
Whovians is a term used for Doctor Who Fans.
Only by people who want a punch in the face.
teacake wrote:
They call themselves that all the time.
I've also heard 'Whovian' used many times. Seems a bit awkward though.
"Pitching is simple -- cheese for the kitchen and a yakker for the kudo." - Dennis Eckersley
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