text:
We Guarantee The Lowest Total Tire Price! On The Rim & Out The Door® Pricing. The No Hassle Buying Experi... more
We Guarantee The Lowest Total Tire Price! On The Rim & Out The Door® Pricing. The No Hassle Buying Experi... more
We Guarantee The Lowest Total Tire Price! All Inclusive Pricing. The No Hassle Buying Experience. more
Drive a little smarter.®Visit our website for available auto services and online coupons! more
There's more to Just Tires than just tires. more
We are a multi-generational family business that provides a full-service solution for all of your automot... more
When it comes to the performance of your vehicle, you want quality service from trusted experts. We are a... more
Over 5 million top brand tires in stock. Fast free shipping to your local installer. Easy! more
Top Trending Articles
How to Tell if it is Time to Change the Tires...
Many factors contribute to determining when it's time t... more
Normal 0 false false false EN-US X-NONE ...
Driving in the winter can be hazardous if you don&rsquo... more
Practical Tips to Increase Your Gas Mileage
Frustrated with gas prices? Even more frustrated with y... more
How to Keep Your Pet Calm During a Fireworks ...
Keeping your pet safe and calm during fireworks events ... more
Why Exercising in the Morning Burns More Fat
Exercise in the morning for maximum results. Learn how ... more
Highly recommend Law Offices of Nader hasan. He was professional, smart, confident, caring and trustworth... more
Doc is involved in my nutritional alternatives for my fatty liver and my back pain. She is wonderful at m... more
© 2015 Local Corporation - US Patent 7,231,405
Some data provided by Acxiom. Questions and Comments?


text:
Registration takes less than 1 minute.
Pasha Group acquired Horizon Line's Hawaii trade lane operations, while Matson took over the ocean carrier's Alaska business.
President Obama and supporters of the Trans-Pacific Partnership won a procedural battle in Congress with the Senate voting to end a filibuster on fast-track Trade Promotion Authority Tuesday, but the real war may be yet to come.
A fire broke out on the Marshall Islands-flagged Purple Beach, causing the crew to evacuate due to toxic gas and fears of explosion.
Australian logistics and stevedoring firm Asciano, parent of Patrick Stevedoring, has confirmed it received a purchase offer of $6.8 billion from Brookfield.
BPO arrangement allows the express giant, which has been targeted for acquisition by FedEx, to focus on its core activities and customers service.
While many airfreight carriers around the globe saw a slowdown in growth, Middle Eastern cargo lines reported a drastic increase in airfreight volumes.

subset: commoncrawl


text: Publications Search Results Users may wish to consult What's Included in the Bibliography. Results 1 - 1 Results per page: 25 50 100 Sort by: Pub Date (newest) Title Recent Additions Author Clear All Pub. Year Citation Cubbin, C., Brindis, C.D., Santelli, J., Jain, S., Braveman, P. Neighborhood Poverty and Initiation of Sexual Intercourse among Adolescents: Mediating and Moderating Effects. . Export Options: RIS EndNote XML [1]


text:
More about this preview.

Change DPI Base size is 150
Explore Our fabrics
Optional Filters
I'm going to use this fabric to make...
I'm looking for a fabric that is...
Receive our weekly email with news about contests, giveaways and prodution releases.
Email Address
No Thanks
© 2008-2015 Spoonflower Inc


text:
Press release from Business Wire
ConAgra Foods Announces Dividend Payment
Tuesday, July 12, 2011
ConAgra Foods Announces Dividend Payment08:09 EDT Tuesday, July 12, 2011 OMAHA, Neb. (Business Wire) -- The board of directors of ConAgra Foods, Inc., (NYSE: CAG) approved a dividend payment of $0.23 per common share to be paid on Sept. 1, 2011, to stockholders of record at the close of business on July 29, 2011. ConAgra Foods, Inc., (NYSE: CAG) is one of North America's leading food companies, with brands in 97 percent of America's households. Consumers find Banquet, Chef Boyardee, Egg Beaters, Healthy Choice, Hebrew National, Hunt's, Marie Callender's, Orville Redenbacher's, PAM, Peter Pan, Reddi-wip, Slim Jim, Snack Pack and many other ConAgra Foods brands in grocery, convenience, mass merchandise and club stores. ConAgra Foods also has a strong business-to-business presence, supplying frozen potato and sweet potato products as well as other vegetable, spice and grain products to a variety of well-known restaurants, foodservice operators and commercial customers. For more information, please visit us at www.conagrafoods.com. ConAgra Foods, Inc.MediaTeresa Paulsen, 402-240-5210Vice President, Corporate email@example.com Klinefelter, 402-240-4154Vice President, Investor email@example.com


text:
The Snail and the WhalePaperback
List price $6.99
You save $0.51 (7%)
Free delivery worldwide
Dispatched in 3 business days
When will my order arrive?
Additional formats available
- Format
- Hardback $15.78
- Publisher: PUFFIN
- Format: Paperback | 32 pages
- Dimensions: 249mm x 274mm x 5mm | 159g
- Publication date: 4 June 2006
- Publication City/Country: New York, NY
- Edition statement: Reprint
- Sales rank: 14
Product description
When a tiny snail meets a humpback whale, the two travel together to far-off lands. It's a dream come true for the snail, who has never left home before. But when the whale swims too close to shore, will the snail be able to save her new friend?
Add item to wishlist
Other people who viewed this bought:
Room on the Broom
USD$6.23 - Save $0.76 10% off - RRP $6.99
Giraffes Can't Dance
USD$9.00 - Save $1.89 17% off - RRP $10.89
The Gruffalo's Child
USD$6.98 - Save $0.01 - RRP $6.99
Stick Man
USD$7.60 - Save $3.29 30% off - RRP $10.89
Dear Zoo
USD$5.61 - Save $1.35 19% off - RRP $6.96
USD$8.44 - Save $2.45 22% off - RRP $10.89
Other books in this category
The Day the Crayons Quit
USD$9.58 - Save $1.31 12% off - RRP $10.89
Room on the Broom
USD$6.23 - Save $0.76 10% off - RRP $6.99
Possum Magic
USD$6.99 - Save $2.36 25% off - RRP $9.35
Ten Little Fingers and Ten Littl...
USD$8.52 - Save $0.81 (8%) - RRP $9.33
Stick Man
USD$7.60 - Save $3.29 30% off - RRP $10.89
USD$8.89 - Save $2.00 18% off - RRP $10.89
- Categories:
Author information
Julia Donaldson lives in Glasgow, Scotland.
Customer reviews
By Pavel Ondrejovic 23 May 2014
It's a lovely, rhymed story that both we as parents and the kids love ;) Beautiful illustrations, captivating flow of rhymes. We certainly recommend.
By Alison Kappler 27 Oct 2013
This is a fantastic book! My 3 year old boy absolutely loves it, as do I! It has wonderful rhymes and beautiful pictures, which both enhance an already charming story.
By Sarah Wilcox 19 Aug 2010
This is a beautiful story much loved by my son. I bought a copy as a present for a friend and was disappointed to discover when it arrived that it is a US edition, even though this is not marked on the description. Look out for US spelling "gray" rather than "grey" and the snail "hitching a ride" rather than "hitching a lift".
Review quote
"Along with providing a resonant environmental message, the story lightly demonstrates that friendships come in all shapes and sizes. Publishers Weekly, starred review"


text:
Does magic mouse work fully in VMWare Fusion 3.0.1
Before I purchase a magic mouse, I just want to make sure that it works fully and cooperatively with latest Mac OSX 10.6.2 and VMWare Fusion 3.0.1 ? No gueses, Just anyone with same setup. Running Windows XP, with the above Mac os and Fusion version. ALL features work. ??
- Asked by Donna V from Camas
- Flag as inappropriate (Does magic mouse work fully in VMWare Fusion 3.0.1)
1 Answer from the Community
Best Answer:
Most features work, scrolling up and down works fine, scrolling side to side does not on Windows. I do love the mouse and am very happy with my purchase.
- Answered by Stephen M from Olathe
- Flag as inappropriate Answer 1
Related Questions
Will the Magic Trackpad work with VMware Fusion and Windows 7 64bit. What functionality works and doesn't work with this combination?
- Asked by Christopher A from Phoenix
- Aug 2, 2010
- Flag as inappropriate (Will the Magic Trackpad work with VMware Fusion and Windows 7 64bit. What functionality works and doesn't work with this combination?)
- Asked about: Magic Trackpad
vmware fusion
- Asked by Nivil L
- May 24, 2012
- Flag as inappropriate (vmware fusion)
- Asked about: Mac
No answers yet
does Lion 10.7 support VMware fusion?
- Asked by Norma P from Ketchikan
- Jan 10, 2014
- Flag as inappropriate (does Lion 10.7 support VMware fusion?)
- Asked about: OS X Lion (10.7)


text:
Movies Distributed by ABC Family
List of ABC Family movies. Famous and notable films distributed by ABC Family are listed below, along with release information. ABC Family films are listed below with ABC Family movie posters when available. If the film was distributed by ABC Family, then it is on this list. Every film on this list wasn't produced by the distribution house of ABC Family, but only distributed. Although some places do both, this list is only a list of movies distributed by ABC Family. Movies list can be sorted by any column. This list has a variety of items, like Snowglobe and Crimes Of Fashion, in it. (28 items)
- 1
Au Pair John Rhys-Davies, Richard Riehle, Gregory Harrison
- 2
Au Pair II Gregory Harrison, Robin Dunne, Rachel York
- 3
Beautiful Girl Fran Drescher, Marissa Jaret Winokur, Brooke D'Orsay
- 4
Beauty & the Briefcase Hilary Duff, Jaime Pressly, Jennifer Coolidge
- 5
Christmas Caper Shannen Doherty, Ty Olsson, Tara Lynn Wilson
- 6
Christmas Every Day Erik von Detten
- 7
Christmas in Boston Marla Sokoloff, Patrick J. Adams
- 8
Crimes Of Fashion Megan Fox, Kaley Cuoco, Dominic Chianese
- 9
Everything You Want Shiri Appleby, Edie McClurg, Nick Zano
- 10
Free Fall Jaclyn Smith, Hayden Christensen, Bruce Boxleitner
- 11
Holiday in Handcuffs Melissa Joan Hart, Mario López, Markie Post
- 12
Legally Blondes Chloe Bridges, Curtis Armstrong, Bobby Campo
- 13
Love Rules Marilu Henner, Maggie Lawson, Joey Lawrence
- 14
Lucky 7 Patrick Dempsey, Kimberly Williams-Paisley, Gail O'Grady
- 15
My Fake Fiancé Melissa Joan Hart, Joey Lawrence, Diane Neal
- 16
National Lampoon's Golf Punks Tom Arnold, James Kirk, Neil Denis
- 17
Princess Nicole Gale Anderson, Nora Zehetner, Devon Bostick
- 18
Revenge of the Bridesmaids Raven-Symoné, Joanna García, Beth Broderick
- 19
Santa Baby Jenny McCarthy, Michael Moriarty, Kandyse McClure
- 20
Santa Baby 2 Jenny McCarthy, Kandyse McClure, Paul Sorvino
- 21
See Jane Date Charisma Carpenter, Rachelle Lefevre, Zachary Levi
- 22
Snowglobe Christina Milian, Lorraine Bracco, Matt Keeslar
- 23
Taking Five Alona Tal, Christy Carlson Romano, Daniella Monet
- 24
Teen Spirit Cassie Scerbo, Chris Zylka, Rhoda Griffis
- 25
The Christmas List Stella Stevens, Mimi Rogers, Enuka Okuma
- 26
The Ghosts of Christmas Eve Jewel, Ossie Davis, Allie Sheridan
- 27
The Initiation of Sarah Summer Glau, Jennifer Tilly, Morgan Fairchild
- 28
added by GaileW.Tolzman
August Rush Robin Williams, Keri Russell, Jonathan Rhys Meyers


text:
Current Articles
RSS Feed
You have six months to meet your goals for 2015. What do you need to do?
That's one of the questions that forces Bank Relationship Managers to think. Unfortunately, thinking consumes both time and energy so most of us tend to avoid it. (One of the things a teacher of mine used to say was that "Most people would rather die than think--and most do.")
So if you had to think about your sales prowess--as if your professional future really depended on it--what should you focus on? Here's a quick checklist for starters:
1. How is your performance year to date? How do you stack up vs. your peers? How are you doing in retaining and expanding relationships with your key and high potential customers? What does your pipeline look like now and 3 or 6 months down the road? Are you making progress with your key prospects? Where do you see the biggest opportunities for growth? What are the major challenges you face?
2. What do you need to work on to improve your results? Be honest. Is it better product knowledge? A more focused prospecting approach? Quality time to prepare for upcoming calls? A better working relationship with your administrative assistant? A consistent follow-up plan to stay top of mind with your COIs and prospects?
3. Who can help you get to the next level? The obvious answer is your sales manager, but think about where you might find other allies. Are there customers who could refer you to other businesses? Do you need to do a better job of networking internally with product partners in cash management or private banking or capital markets? Can the Executive Director of that trade association of manufacturers become a referral source?
4. What's your plan? Get specific. Write it down. Make sure that you get feedback from your personal board of advisors (e.g. your spouse, your boss, any of your good friends or coworkers who want you to succeed, etc.)
For most of us this is not a 20 minute exercise. But if you've only got 20 minutes to start the process today, do it. Your results in 2015 (and in 2016) may well hinge on it.
Here are some prospecting resources from MZ Bierly Consulting.
1. How to Build a Business Network eBook
2. Recorded and Live Webinars
If you liked this post, feel free to share it with your network via email, LinkedIn or Twitter.
What do you think? What can bankers do to improve their success in prospecting? Please share your advice, insights, and experiences in the COMMENTS section below.
Looking for more tools to help you with prospecting? Check out the following resources:
How to Qualify Prospects Quickly
Leveraging Your Network in Prospecting
Keys to an Effective First Call on a Prospect
Go to http://mzbierlyconsulting.webex.com for a complete list of our recorded webinars on prospecting.
Free Webinar: Alternative Lending and Banks with Charles Wendel Alternative lending companies continue to focus on the business banking space. Increasingly, banks are evaluating how to cooperate with these players since they offer streamlined processes and enhanced risk management, both of which can turn small business lending into a profitable opportunity for banks. This webinar will summarize the current state of alternative lending, the different lending approaches, the major players and how they work with banks as well as developing trends. It will also discuss the key criteria that banks need to consider when they evaluate whether and how to work with these new lenders. Date: Friday, August 14, 2015 Time: 11:00 am, Eastern Daylight Time (New York, GMT-04:00) REGISTER HERE
If you liked this post share it with others on LinkedIn, Twitter and by email.
Many senior bank executives question whether their sales leaders have what it takes to get the job done. Some banks are redoubling their efforts to train and coach their first-line sales leaders on how to develop their commercial and small business teams. Others are actively recruiting management talent. The smart ones are doing both.
If you're a sales leader, here are 10 mistakes you don't want to make:
What do you think? What can banks do to improve the performance of sales leaders? Please share your advice, insights, and experiences in the COMMENTS area below...
Date: Friday, August 14, 2015 Time: 11:00 am, Eastern Daylight Time (New York, GMT-04:00) REGISTER HERE
Cold calling is sometimes a banker's best option. For some inexperienced branch managers and business bankers that can lead to cold call reluctance. Here's what to do if you find yourself procrastinating or dreading the thought of making a cold call to a prospect.
If you would like to download a transcript of this video, click on the link below.
Cold Call Reluctance
Free Resource for Bank Sales Managers: Download your copy of "Coaching the Prospecting Process: Tips for Sales Managers" by going here.
Archived Webinars on Prospecting: Go to http://mzbierlyconsulting.webex.com for a complete list of our recorded webinars on prospecting.
What do you think? Please share your advice, insights, and experiences in the COMMENTS area below...
If you liked this post, share it with your colleagues by email and on LinkedIn.
What is it about the number 3? Some people consider it lucky. It is sacred in many religions. In some traditions 3 is considered the number of harmony, wisdom and understanding.
Heroes are often offered three choices or three tests. Others overcome difficulties on the third try.
Counting to three is common in situations where a group of people wish to perform an act together. (Now, on the count of three, everybody pull!).
Basketball coaches know that three word commands are easy to remember. See the ball. Slide your feet. Everybody box out.
Well, on 6/3/2015 I'm going to suggest to bankers that 6 things in 3 word snippets might change your business development results this year. Here they are:
If one of these looks like it will help you make progress in landing more prospects and meeting your sales goals, do something with it—in the next 3 days!
Final tip: Be sure to click on the hyperlinks for more details.
The novel Anna Karenina begins with the observation that "Happy families are all alike; each unhappy family is unhappy in its own particular way." That's probably true of sales teams as well, whether they're composed of bankers or pharmaceutical sales representatives.
Having spent time recently with both happy and unhappy bankers, though, I've been struck by how the mood of teams is determined to a large degree by their leaders. Bank sales managers can have a huge impact on the morale and performance of front-line personnel.
What specifically do the best sales leaders do? What are the secrets to their success? Here's a partial list:
10. They keep things in perspective. They know that their attitude has a huge impact on the success of their team.
Post Script: I shared this list with two sales leaders who have been successful building what I would call happy (and successful) commercial teams. Here are their comments:
Bank Sales Leader #1: "I would add that joint calling lets the managers demonstrate that they are also using the sales tools which they profess are so important. Additionally, this simple activity can reinforce that the program works. I also believe that this demonstrates that the manager is not afraid to get into the action, specifically if you are calling on prospects or customers with opportunities in the pipeline. Sales teams need to know that we do what we preach as managers.
Lastly, if the manager demonstrates energy and positive attitude, this can "rub off" on the RM. I've found that the manager's perspective becomes the RM's perspective rather quickly. If the manger is down, the staff is down. If the manager believes "we can do it", the staff generally responds in kind."
Bank Sales Leader #2: "I absolutely agree with this article. One very key component that is missing and I would add is that the sales leader must always be positive. Even in difficult times, challenging credit criteria and demanding customers and prospects the leader needs to be a partial spin doctor and always see the light at the end of the tunnel for the team. The positive attitude and confidence of a leader are contagious."
OK, sales leaders, what else would you add? Share your thoughts in the space provided below or email me at email@example.com.
Question: I am currently considering joining a networking group. This group limits members to one from each industry, however, when I attended the first introductory meeting there were three other bankers who wanted to join. I wonder how this group will approve applications if we all apply? The group appears to be a well-organized one and the leader seems genuine in getting referrals going.
To download a podcast with my answer--or a transcript of it--click on the link below.
Q&A on Networking Groups
Interested in a series of fast-paced refreshers on prospecting? Check out any of our 10 archived webinars on Prospecting Strategies:
Building a Good Prospect List
Leveraging Your Network to Get in the Door
How to Use Your LinkedIn Network in Prospecting
Preparing for First Calls on Prospects
Industry Research as a Differentiator
Business Operations Meetings: Building Strong Relationships with Business Owners
Delving into Financial Operations: Selling to Financial Change
The First 3 Calls on Prospects
Following Up on Proposal – Persistent or Pest?
Getting the Most Out of Networking Events
Go to http://mzbierlyconsulting.webex.com or contact Susan Lersch at 610-296-4771 for more information and team discounts.
Tax season is over. It is probably OK to start calling on CPAs again in search of referrals. But before you do, here are 6 reasons why you ought to be asking some of your clients for introductions too:
For more thoughts on why you ought to be spending more time with your satisfied clients, check out this video.
Looking for specific tips on how to ask for referrals? Check out this article How to Ask Customers for Referrals
You might also be interested in signing up for one of our recorded webinars on the topic at http://mzbierlyconsulting.webex.com
If you liked this post, share it with your colleagues via email or on LinkedIn.
Editor's note: This blog post is one of our most read. It may be a stretch to compare it to a classic Mustang, but that's a chance we'll take.
With apologies to Stephen Covey, here are my "7 Habits of Highly Unproductive Salespeople (banker version)":1. Be reactive. Wait for what walks in the door. Don't ask your satisfied customers for referrals—they know you provide great service and will send you any leads they have.2. Put (anything other than customers) first. Problem loan reports. Compliance issues. Operational matters. No experienced banker gets canned for failing to make his sales goals. 3. Don't worry about preparing for calls. Industry research--who has time for that? Developing specific questions for a first meeting with a prospect? Just ask 'em to tell you about their business.4. Strategize alone. Your Sales Manager (a) doesn't know your customers/ prospects well; (b) doesn't have time; and/ or (c) probably wouldn't add much value if you talked about your strategy.5. Think about what you need to sell first, not what your customers and prospects need. You've got goals to make. 6. Don't make time for professional development. Training is for "junior" people, not veterans like you. Heck, if you're like most commercial bankers, you believe that you're in the top 20% of your peer group when it comes to business development skills. This is known in some circles as the Lake Wobegon effect—where all the children are above average. (Special offer: If you'd like to assess which stage your relationship building skills are in, go to The Progression of Relationship Development Skills )
7. Keep reminding yourself that knowing how to do something (like prospecting, say) is the same thing as doing it. And, if anybody questions your current results, you can come up with any number of excuses ("Our credit policy is too restrictive." "We're not even close to market pricing today." "We need to do more advertising/ build more branches/get more creative on structuring deals, etc.")
To read more articles on sales leadership topics visit our blog at Bank Sales Corner Blog
Feel free to share this post with others in your organization who might benefit or via LinkedIn.
A few years ago Greenwich Research reported that almost half of all business customers couldn't recall any calls in which their bankers had furnished a new idea. When asked what the most memorable topics discussed were in their meetings with bankers, the most common response was "nothing."
What then would business owners like to talk about? To find out the answers, watch this brief video.
To download a transcription of this video, click on the link below.
Would Your Prospects Pay for your Next Call?
More resources--Check out these mp3s on:
Acquiring New Relationships http://www.mzbierlyconsulting.com/acquiring-new-relationships/
Building Your Personal Brand http://www.mzbierlyconsulting.com/building-your-personal-brand-podcast
© 2015 110 N. Phoenixville Pike, Suite 200, Malvern, PA 19355 • 610.296.4772 • Fax: 610.296.4775


text:
Sculpt and tone your midsection by strengthening your abdominal and lower back muscles. Learn correct body position and techniques that will get you better results.
Experience a total body workout while learning boxing combinations and cardio routines.
Take sculpting to the next level! High-intensity weight training techniques that utilize dumbbells, body bars, resistance-tubing and steps. Abs included.
H.E.A.T.- (High Energy Athletic Training)Workouts designed to improve your agility, strength, balance, and cardiovascular performance. Training may include weights, steps, balls and Bosu. Abs included.
A dynamic mind-body conditioning class based on a unique system of stretching and strengthening exercises developed over 90 years ago by Joseph H. Pilates. This workout will strengthen and tone muscles; improve posture, flexibility and balance; unite body and mind; and create a more streamlined shape.
Focuses on abdominals, hips, thighs and glutes. This workout includes mat work, small weights and other props. Results Barre combines elements of Pilates, yoga, dance and various Barre methods.
Results Flow: A flow-based class modeled on the sun salutations that links standing and seated postures, backbends and inversions with the breath in evenly-paced, straightforward sequences. Appropriate for all levels.
Burn an average of 500 calories while getting a heart-pounding, low-impact workout. Set your own pace as you learn proper form and riding technique. This cardio class accommodates all fitness levels.
Arthur is an AFAA certified group fitness instructor with 20 years of experience. Energy describes Arthur. He motivates students with his humor and passion for fitness.


text: African-American Churches Weigh Gospel Debate July 31, 2005 The rise of "mega-churches" in the African-American community has helped draw people back to religion. But some traditional black pastors find the growth of these churches worrisome, saying their leaders focus on messages of personal prosperity and are neglecting the civil rights struggle. Listen Loading… Playlist Download Embed Embed Close embed modal <iframe src="http://www.npr.org/player/embed/4779412/4779413" width="100%" height="290" frameborder="0" scrolling="no">


text:
Supernatural Season 3 Reviews
Children get super creepy and Dean runs into an old flame in "The Kids Are Alright." Read More... http://www.ign.com/articles/2015/07/01/supernatural-the-kids-are-alright-flashback-review More »
We begin our look back at Supernatural, as Sam and Dean dealt with the Devil's Gate and met a mysterious new ally. Read More... http://www.ign.com/articles/2015/06/24/supernatural-the-magnificent-seven-flashbac More »
The part when dean had the rabbit's foot and saving Sam is too funny ( I am batman.) priceless More »
I loved this episode, this blond hot chick/Demon "Ruby" seems to be nice to Sammy and Dean, but we'll have wait to see what happens in the rest of this season, More »


text:
The VIP Experience at Universal Studios Hollywood
Read what other Viator.com travelers think about the The VIP Experience at Universal Studios Hollywood. What they loved, what they liked and what they think could be improved, it's all here to help you make the most of your next trip.
Click here for tour details, schedule and pricing
Darren H
United Kingdom
Great tour, definitely worth the money as you get to access areas others don't, lunch was superb, easily the best meal I've had during my time in the USA, one thing I didn't know was that if you arrive early for your tour you get a complimentary breakfast buffet in the Universal VIP lounge, Top Day
This was my favorite part of my vacation. Everything was perfect and totally worth the price of the tour.
This was my favorite day so far on my holiday the VIP experience at universal studios was absolutely amazing. Would highly recommend doing this. My tour guide was brilliant she had a whole day planned for us we got to see the whole park she took us on all the rides. it was absolutely amazing
I would recommend this tour to anyone. Lots of interesting information about the movies and how they are made and why the cost so much. The food was fantastic and going straight to the front of the line was a Hugh factor. Got to see everything once and more fun after on rides we really enjoyed.
Fantastic way to see EVERYTHING at Universal in just one day. Dawn our guide was informative and kept everyone moving at a good rate. The VIP pass to rides is great as you w
Could queue for hours without them. The 4D rides are mind blowing.
The tour was well organised with time to enjoy the major attractions but also have personal time. We started with a morning tea followed by a personally narrated tour of the studio in luxury coaches. The guides were witty and knowledgable. Lunch was wonderful, rides and shows exciting. Great tour
Well worth the extra money! From start to finish we were treated like VIPs and our guide was knowledgable and planned our route of the park perfectly with 2 hour free time at the end. He was happy to take our photos around the studios and even looked after our bags whilst we went on the rides.
This tour was amazing!
Truly a VIP experience, worth every single cent.
VIP is the only way
Don't even think otherwise
this is the only way to do universal studios absoulutly amazing
Excellent would do this again, good value for money when you consider you can use the front of line card all day, our tour guide Robert was an extra treat.
Totally fantastic day, we were well looked after and made to feel like true VIP's. Our guide Doug was great fun.
Tunisia was an excellent guide. Well worth the expense
Great Experience, saw the whole park and got a great tour
A little bit pricey but worth it as we cover all the rides before we leave as we only have a day there.
Best way to experience Universal Studios. Starting from the amazing breakfast to the guided, front of line experience. Would recomend
United Kingdom
This is a must do at universal the backlot was fantastic, the guide Larry was friendly and brilliant at explaining and very knowledgeable about universal. Having food in with the price was a bonus and also having front line passes and vip seating at the shows was great. The whole experience was amazing from start to finish I would definitely do this again
The VIP experience was well worth the money for people who want to see as much as possible in the one day while being fed with nibbles for breakfast and exceptional buffet at lunch ,with personal commentary from knowledgeable guides. Front of line access to all rides and plenty of free time at the end to wander and souvenir hunt.
Great tour!! Very worth the $$$ our guide was amazing and we got to learn a lot about movies and how they're made. The lunch was fantastic!
A truly amazing day well worth paying the extra for the vip tour you got to see both sides of universal studios the behind the scenes and front of queue on all the rides the Buffet lunch was amazing there was so much choice I would say it was definitely the best food on the whole park. Our guide for the day was Mark he was very informative and really knew stuff definitely a trip to do if you are ever in Los Angeles
This is definitely the best way to visit Universal Studios! We had a lovely breakfast with plenty of choice and was then met by our guide for the day. He took us to the different rides and walked us straight to the front of the queues, he also gave us plenty of information. We then stopped for a brilliant buffet lunch, again with plenty of choice and the food was delicious. Then it was time for the studio tour where we saw the different sets and got to have a wander around them, it was interesting to hear the different films sets had been used in that you didn't realise. We then had the rest of the day to ourselves and it was a definite bonus to still be able to jump the queues for rides. It was a brilliant way to spend my sister's birthday and I would recommend it to anyone visiting Universal Studios, it is well worth the money!
OMG! One of the best tours I did on my trip. Skip the line pass with this is amazing. Not waiting for rides - best way to enjoy the most of your day and the attractions at Universal Studios. VIP seating is also well worth it as once again you don't have to go and start lining up half hour or more before the performance / show and still get the best spots in the house! Great use of your time here. So much fun on this tour! You also get to walk around the sets and see "hot sets" as part of this tour.
The VIP Experience will make you really like VIP Person the lounge, the buffet lunch with 5 star food, just a small group of people with tour guide, you can stop and take picture on location, you don't have to queued when entering the attraction especially for the Transformers very long que. Highly recommended if you want to feel everything in 1 day.
I could not recommend this tour highly enough, it was absolutely the highlight of our trip! It is worth getting the VIP package as parking is included as well as breakfast, lunch and bottles of water. Unless you choose to buy souvenirs or alcohol you won't need to spend any more money in the park. The tour was fantastic, our guide Tanisha was with us for the whole day and was really lovely. We had front of line passes for all the rides to go on them as often as we liked as well as reserved seats at the shows, the food was delicious with a really great selection... I could go on and on! A really excellent tour!


text:
Check out our Upcoming Concerts!
Hosts / Schedule
The Morning Point w/Mike and Woo Woo
Woo Woo
Mike Powers
Barry McKay
Don London
Listen Live
The Point's Vault
Listen On Your Phone
Photo Gallery
the Point after
Paws and Claws with Selina
Point TV Videos
Fitness Friday
Point Events
Mike Powers
The Point's Feed
Barry McKay
Whats the one thing that annoys people the MOST?
by Barry
posted May 8 2013 1:00PM
A slow computer! And one in three people have YELLED at their computer. The rest of the top five annoying things are: People who leave clothes on the floor . . . a sink full of dirty dishes . . . a leaky faucet . . . and leaving the toilet seat in the wrong position.
Here's the rest of the survey!
1. A slow computer. One in three people have YELLED at their computer . . . and 6% of people say that they feel better after HITTING their computer.
2. People who leave their clothes on the floor.
3. A sink full of dirty dishes.
4. Leaky faucets.
5. Leaving the toilet seat in the wrong position.
6. Your significant other.
7. Annoying neighbors.
8. Children.
9. Pets.
Back To Blog
What's the one thing that annoys people the MOST?
Please Enter Your Comments Below
Title :
Comment :
Please enter the text as it is shown in the box below :
Refresh Image
Pick Your Playlist
Latest HollyWooWoo Reports
advertise with us
Recent Blog Posts
So That's How You Make Twins...
Mike Stanz
Jul 3
Barack Obama, the Voice of America
Mike Stanz
Jul 3
Uncle Sam's Crying a Little
Mike Stanz
Jul 3
Bruce Springsteen Jumps On Brian Wilson's Encore
Mike Stanz
Jul 3
Thank You, Donald Trump, from America
Mike Stanz
Jul 3
Now You Can Stuff Your Own Donnie T
Mike Stanz
Jul 3
Liev Schreiber Talks Voiceovers
Mike Stanz
Jul 3
"The Authorities Would Tell Us!"
Mike Stanz
Jul 3
Jul 2015
Jun 2015
May 2015
Apr 2015
Mar 2015
Feb 2015


text:
search for a card
Result of the search for the collectible card:
The following 6 users match with the requested search. Click on their names to contact them.
For some advice on how to trade cards click here.
- elgalactiko LV5 (Chile-Maipo)
- german_lopez_31 LV4 (Chile-Santiago)
- undeeeer LV2 (Chile-Cachapoal)
- polilla LV2 (Chile-Santiago)
- totosaralegui69 LV2 (Chile-Santiago)
- jonaestepp LV1 (Chile-Ñuble)
When you register you will be able to search for all your cards in one go, and we will send you any new results of the search every day by email.


text:
The Top 10 In Action Sports
01Brick By Brick: Camping Lego Style
02Booze and Bikes: 14 Tours That Let Combine Two of the Best Things in the World
03Who Needs Hollywood? These Incredible Videos Were All Filmed on the Simplest Cameras
04Sam Pilgrim vs. The World: The Champion Aiming To Reclaim His Crown In A New Era Of Biking
05Eccentric Adventures Episode 2: Britain's Most Brutal Illegal Event is All About Cheese
06A Journey Into Darkness: We Went Mountain Biking in the Abandoned Mines of Slovenia
07After the Apocalypse: Inside The World's Most Breathtaking Ghost Towns
08Debate: Let's Stop Hating On Parents Who Want To Raise Adventurous Kids
09Guns, Roses, And Eating Insects: Adventure Cycling in Vietnam
10From Intense Trails to Intensive Care: Dicing with Death In a Mountain Biking Paradise
The Scrum Tilly Lush- Teaser # 2
A Skateboarding video by wallnut - 24,414 views
Teaser # 2 for the all super 8 film that explores several European city's scenes. Featuring Love Eneroth in Stockholm, Conhuir Lynn & Denis Lynn in Belfast, Wieger Van Wageningen in Amsterdam, Lennie Burmiester in Berlin, Daniel Lebron & Vinnie Bressol in Barcelona, Nick Jensen in London, Soy Panday & Vivien Feil in Paris, Al Collins, Wayne Gallagher & Paddy Clear in Dublin, Janne Saario in Helsinki, Per Magnusson in Malmo, & Danny Wainwright in Bristol.
updates: aulpups.blogspot.com
What Do You Think?
Get the best of Mpora straight to your inbox


text:
Greece has dominated the news lately but there are a few key players that you should know. Check out this list!» Read More
Check-in is only the start of hotel spending. Guests are paying more for cocktails, massages and other extras.
Technology will have a major impact on advisor firms as they look to leverage solutions to provide better experiences for clients.
When it comes to vacation nightmares, renting a home or apartment from a scammer has to be near the top of the list.
The crowdfunding company Indiegogo hopes to cash in on equity crowdfunding once regulations are in place.
Despite optimism surrounding the U.S. recovery, one asset management firm has signaled that this could mean a gloomy future for the rest of the world.
Since 1969, millions of cars have been recalled after being deemed threats. CNBC.com takes a look at these cars, from the 1971 Pinto to this year's growing list of recalls.
Stanford's divestment from coal is just the beginning of investors taking climate change seriously, according to a senior Wall Street risk pro.
The franchise model continues to grow in the US, and more owners are now making millions from little-known franchising concepts.
The Veterans health care system has come under fire as officials reap big bonuses while patients suffer. CNBC's Dina Gusovsky investigates.
The "golden years" could be pretty rocky for one in four Americans, and roughly a third of young "Millennials", if they don't sock away some cash.
Collectors can breathe easy as Hess announced it will continue making its toy trucks after the sale of its retail arm to Marathon Petroleum.
Can powerful brands that court socially-conscious consumers stretch their values to include a higher minimum wage?
Correction is the call du jour in the stock market these days, and it's getting more and more converts.
Video game makers like EA look for ways around high taxes to build a big following in Brazil ahead of the World Cup.
Laws designed to combat climate change won't do anything but hurt economic growth, said Sen. Marco Rubio. He also weighed in on retirement savings.
Some of high-ranking retired military brass are advocating for a radical way to bolster national security: Improve the fitness of children
Eight owners of unsung franchises generating more than $1 million in annual sales offer lessons learned on building a thriving franchise.
Despite some financial and legal challenges, bike-sharing programs continue to roll out in cities throughout North America.
Whether you win a lottery or get picked in the NFL draft, careful planning is required to avoid going bankrupt, as do most suddenly rich.
Investors could have profited from Fed policy announcements by gaining access to information ahead of public release, according to new research.


text:
5 Tips to Be a Good Team Player
By Kyle Ohman
A team with unselfish players who support each other will generally perform better than a group of individuals who doesn't play as a team. Learn how t
Sign up for a college baseball camp, find tips on how to improve your baseball skills with training, and learn how to get recruited.
Adding family members lets ACTIVE find events specific to your family's interests.


text:
Recent Appearances
How Business Leaders Can Save Education
The Heritage Foundation sponsored a forum on methods to improve inner city schools and to assess what role American business…
African-Americans and Education
The speakers described ways of improving inner city schools. Issues raised in the forum included the participation of…
Equal Opportunity in Education
Assistant Secretary Williams talked about his past two years in the Department of Education. He spoke about the issues…
Education Department Office of Civil Rights
The committee heard testimony on the Department of Education's Office of Civil Rights, an agency designed to enforce…
Appearance Stats
- 1992 Videos: 3 See all videos
- 1991 Videos: 3 See all videos
- 1990 Videos: 1 See all videos


text:
By clicking the "Submit" button, you agree to Avvo's Terms of Use. Please see our Community Guidelines and Privacy Policy for information on posting to Avvo and how we collect, use, and share information you provide to us.
501 Executive Center Boulevard Suite 100El Paso, TX 79902
Licensed for 21 years
Go back to Joseph's profile.


text:
Please pray for my daughter Allyson and her husband Michael as the struggle to obtain financial solvency.
Pray along with our community, or submit your own.The prayers on this page are also being prayed by The Episcopal Community
- Cindy R.
For successful surgery for Ginny and continued healing.
- Nancy K
for strength and gainful employment.
- H. Black
I need emergency prayers for my Facebook friend Julianna in Oklahoma. Her mother needs cancer surgery and is short $2000. which must be raised by Wednesday. Ask God to provide just enough just in time for Juliana.
- Kevin Y.
This is a prayer request for an increase in Latino membership at Iglesia Episcopal San Cristobal in Orlando,Florida.
- Alejandro R.
For the safe trip my children and husband are getting ready to take.
- Buffy F.
I pray that me and my family fin a house to live in,in our old town
- Cody.W
Nana to help her be calm and allow god to guide her threw her final stages of life. Grandad know your god and family will help and carry you.
- Jessica e.
Pray for patience and mercy
- jennifer h.


text:
Southaven, MS Extraordinary Women Conference 2015
- Religion & Spirituality
- 4560 Venture Dr Southaven, MS 38671, US
Time Management Skills for Interns
Office of Academic Internships
Thursday, October 31, 2013 from 2:45 PM to 3:45 PM (PDT)
The Experiential Learning Lab presents University of Memphis students the opportunity to learn the importance of time management skills as interns. All majors are welcome. Don't forget to RSVP. We hope to see you there!
Join millions of people on Eventbrite.


text:
Upgrade Gt-i9100g To Android 4 2
If you searching for Upgrade Gt-i9100g To Android 4 2, you come to the right place. Here you can read or download Upgrade Gt-i9100g To Android 4 2 directly from the official website. Free download APK file for your Android device. This android applications, android games, android themes, android how to troubleshooting or android apk is provided by the community, and you will read or download from their server.
Read or Download Upgrade Gt-i9100g To Android 4 2
Android - 4.4 kitkat, Bluetooth map support android now supports the message access profile (map) so bluetooth-enabled cars can exchange messages with your. Android 4.0 upgrade list: is your device getting ice cream, Android 4.0 upgrade list: devices that have received (or are receiving) ice cream sandwich • acer iconia tab a100 [updated 5/1/12]: acer delivered android 4.0 to. Android (operating system) - wikipedia, the free encyclopedia, Android is a mobile operating system (os) based on the linux kernel and currently developed by google. with a user interface based on direct manipulation, android is.
Android 4.1.2 update causing problems for motorola droid, Are you the proud owner of the droid razr or razr maxx? did you update to android 4.1.2 when it became available earlier in the month? is it working perfectly?. Android - 4.3 jelly bean, Dial pad autocomplete just start touching numbers or letters and the dial pad will automatically suggest numbers or names.. Firmware archive | samsung updates, Remember! if the flashing process is interrupted (e.g. with kies) your phone might be very difficult to revive. firmwares provided by samsung-updates.com are not.
The Upgrade Gt-i9100g To Android 4 2 is available for free. We got the link from internet. We are neither affiliated with authors and brands nor responsible for its content and change of content. You can read or download Upgrade Gt-i9100g To Android 4 2 below. Check any other articles by browse all content of this site.
Download page: Upgrade Gt-i9100g To Android 4 2


text:
rdave13 24 Feb 12
Yes, the freebie AV has also been nagging me. Might as well go for it now. If you don't hear from me again you'll know what's happened, killed by an updated version..
This thread is now locked and can not be replied to.
Should I upgrade to Windows 10? 8 reasons why you should upgrade to Windows 10... and 2 why you…
We are being sold the ability to spend money we don't have. And we love it
IKinema aims to banish droopy shoulders and wonky spines in animated CG characters
How to use Apple Music in the UK: Complete guide to Apple Music's features


text:
|Amazon Price
|New from
|Used from
Library Binding
"Please retry"
Audio, Cassette, Audiobook
"Please retry"
Product Details
Would you like to update product info or give feedback on images?
IT'S AMAZING. That book is the best book ever I am completely happy with Piers Anthony's wonderful work I LOVE ITPublished 3 days ago by Kindle Customer
I think this is a very good book, as well as series. It's an intriguing world where all of your traditional tropes are twisted in the best ways. Read morePublished 27 days ago by Joel Dalton
W h at can one say. I've read Plers Xanth series so many times. When I need a break from the serious, or just need a lift, I can always count on PiersPublished 1 month ago by Kenneth. O'Leary
Love this book I only wish it had been put to audio form other than just tapes. And would love to see it as a movie.Published 1 month ago by andrea h kouyate
Ever since first reading this I have always enjoyed the Xanth series of books. The use of puns isn't for everyone but it always seemed clever to me. Read morePublished 1 month ago by Todd C. Young
I'm reading this book with my 6th grade elective class. It uses wonderful puns and idiomatic expressions which many second language learners have trouble with. Read morePublished 1 month ago by Stephen Goodelle
It was funny when Bink got past the shield of instant death
Also it was funny when it said cuss words


text:
on Thursday's show....in film there's Magic Mike, Terminator Genisys and the life & work of Orson Welles and the latest albums from Years and Years, Ezra Foreman and Neil Young
on Thursday's show....in film there's Magic Mike, Terminator Genisys and the life & work of Orson Welles and the latest albums from Years and Years, Ezra Foreman and Neil Young
Daily arts and popular culture show.
Author Jeffrey Deaver on his book 'The Kill Room'. The Publishers are Hodder & Stoughton.
Germaine Greer will be a guest on Arena at The Hay Festival in Kells, Co Meath on Friday June 28th. Questions for Germaine Greer can be e-mailed to firstname.lastname@example.com.
Sonya Kelly on being blue in the face after touring her show 'The Wheelchair On My Face' to over 50 venues. The show is at the Kinsale Arts Festival in Cork on July 10th and 11th - www.kinsaleartsfestival.com.
Michael Hilliard Mulcahy and Don Wycherley on the play 'After Sarah Miles' which will be at The Viking Theatre in Clontarf in Dublin from July 1st to 13th - www.vikingtheatre.ie.
Niall Toner on his book 'The Nuts and Bolts of Song-Writing'. The Publishers are Blackstairs Publishing. www.nialltonerband.com for details on Niall Toner's gig dates.
A play in which an Irish man tells of how his life changed when he went to Senegal has already been a huge success at the Dublin Fringe Festival. It's called 'Gis a shot of your bongos, Mister'. Brian Fleming and Senegalese musician Chico join Seán Rocks. It's part of this week's Big Bang Festival - www.bigbangfestival.com.
Presenter: Seán Rocks
If you require a CD copy of this programme please e-mail email@example.com or click here for RTÉ Archives sales form. Transfer fees and terms and conditions apply.
Presenter: Seán Rocks
Tip: You can drag and scroll the programme grid with your mouse


text:
|Home | About | Journals | Submit | Contact Us | Français
Besides its cooperating effects on stem cell proliferation and survival, Kit ligand (KL) is a potent chemotactic protein. While transwell assays permit studies of the frequency of migrating cells, the lack of direct visualization precludes dynamic chemotaxis studies. In response, we utilize microfluidic chambers that enable direct observation of murine bone marrow-derived mast cells (BMMC) within stable KL gradients. Using this system, individual Kit+ BMMC were quantitatively analyzed for migration speed and directionality during KL-induced chemotaxis. Our results indicated a minimum activating threshold of ~3 ng ml−1 for chemoattraction. Analysis of cells at KL concentrations below 3 ng ml−1 revealed a paradoxical chemorepulsion, which has not been described previously. Unlike chemoattraction, which occurred continuously after an initial time lag, chemorepulsion occurred only during the first 90 minutes of observation. Both chemoattraction and chemorepulsion required the action of G-protein coupled receptors (GPCR), as treatment with pertussis toxin abrogated directed migration. These results differ from previous studies of GPCR-mediated chemotaxis, where chemorepulsion occurred at high ligand concentrations. These data indicate that Kit-mediated chemotaxis is more complex than previously understood, with the involvement of GPCRs in addition to the Kit receptor tyrosine kinase and the presence of both chemoattractive and chemorepellent phases.
Mast cells are important effectors of inflammation, releasing mediators involved in the response to bacterial and helminthic infection, as well as in allergic disease.1–3 Mast cell progenitors are derived from hematopoietic stem cells (HSC) and circulate in the peripheral blood before entry into tissues and differentiation to form tissue-specific mast cell populations.4 The development of mast cells from progenitors is dependent on proliferative and anti-apoptotic signals mediated by the c-kit receptor (Kit) and Kit ligand (KL, aka stem cell factor, SCF).5–7 Two major subsets of mast cells, connective tissue and mucosal, have been described with different functional capacities. Connective tissue mast cells in the skin, peritoneum, and perivascular areas have granules containing tryptase and chymase, while mucosal mast cells in the lungs and intestine express tryptase but not chymase.8 Although they are tissue resident, mature mast cells undergo migratory redistribution in response to various stimuli. For example, parasitic infection in mice causes jejunal mast cells to migrate from the submucosa or crypt areas to the lamina propria and intraepithelial regions.9 Mast cells are responsive to multiple chemotactic stimuli, including KL, IgE and IgE-antigen complexes through the FcεRI, CC, CXC, C, and CX3C chemokines, and non-protein mediators such as sphingosine-1-phosphate, eicosanoids, and adenosine.10 In addition to its known role in mast cell chemotaxis, Kit signaling also results in synergistic activation of FcεRI, resulting in greater degranulation and cytokine release.11 Thus, Kit signaling has critical roles in both the development of mast cell progenitors and the functions of mature mast cells, including chemotaxis and activation.
Kit is a type III receptor tyrosine kinase that is expressed by mast cells at high levels and is also expressed at different levels by a variety of hematopoietic stem and progenitor cell populations, including hematopoietic stem cells, erythroid and myeloid progenitors, lymphoid progenitors and thymocytes. In addition, Kit is expressed in non-hematopoietic lineages, notably embryonic stem cells, germ cells, neural and cardiac progenitors, and interstitial cells of Cajal.12–15 Kit signaling is critical for the development and maintenance of such populations. Mice mutated for Kit have the dominant White spotting defect (W), while those mutated for KL have the phenotypically similar Steel (Sl) defect.16 Both are marked by abnormal melanocyte development and migration, resulting in hypopigmentation, varying degrees of HSC and hematopoietic defects, and impaired fertility.
Studies of chemotaxis in mast cells have been performed with a variety of methods, including transwell assays and measurements of chemotaxis in cells covered by a semisolid medium.17,18 These assays measure net movement of populations of cells and are limited by the lack of direct observation of individual cell behavior and by lack of a stable chemokine gradient. A common challenge in the interpretation of data from these experiments is the confounding effects of chemokinesis (an increase in random motility) and chemotaxis (an increase in directional migration).19 To address these limitations, we directly observed and quantified the chemotactic behavior of individual murine bone marrow-derived mast cells (BMMC) in microfluidic chambers containing stable KL gradients. The results revealed previously undescribed complexities in the mast cell response to KL, namely a concentration- and temporal-dependent bimodal movement. KL concentrations greater than a threshold value of ~3 ng ml−1 resulted in chemoattraction following a concentration-dependent lag time. Unexpectedly, concentrations less than 3 ng ml−1 caused transient mast cell chemorepulsion, i.e. movement away from the KL source, followed by a period of random motility. Both the chemoattractive and chemorepulsive behaviors were found to require the activity of WASP, a regulator of cytoskeletal structure that is a phosphorylation target of activated Kit, as well as G-protein coupled receptors (GPCR). While GPCR are well known chemotactic receptors, they have not been previously implicated in KL-mediated chemotaxis and have not been reported to induce transient chemorepulsion at low chemokine concentrations.
Murine BMMC cultures were established as previously described.20 Bone marrow cells from 4–8 week old C57BL6/J and WASP(−/−) mice21 were cultured for 4–6 weeks in BMMC medium with 20% Fetal Calf Serum (FCS) in the presence of murine IL-3 and KL (20 ng ml−1 each; BioSource International). BMMC medium consisted of RPMI-1640 (Gibco), 1 mM sodium pyruvate (Gibco), 0.1 mM nonessential amino acids (Gibco), and 50 μM β-mercaptoethanol (Thermo Fisher Scientific). Cells were factor deprived for 12 hours prior to chemotaxis assays. For pertussis toxin experiments, 100 ng ml−1 of pertussis toxin (Sigma Aldrich) was added to the factor-depleted medium. After 12 hours of exposure, cells were rinsed in BMMC medium prior to chemotaxis assays. All animal work performed are approved by the Administrative Panel on Laboratory Animal Care (APLAC) at Stanford University.
Transwell migration assays were conducted using 5 μm pore size Transwell devices (Corning, NY). Various concentrations (0, 1, 2, 4, 8, 10, 20, 30, 100 ng ml−1) of recombinant mouse KL were placed into the bottom chamber of the Transwell device, and 2.5 × 105 BMMC in 100 μL medium were placed in the top chamber. After 3 hours of incubation at 37 °C, cells were collected from the bottom chamber and counted using a hemocytometer. Three independent trials using BMMC from three mice were performed.
Devices were designed using AutoCAD software and fabricated from polydimethylsiloxane (PDMS) using standard soft lithography methods.22 The microfluidic device consists of a central cell chamber that extends 4.0 mm in length, 1.0 mm across, and 100 μm in depth. The cell chamber is flanked by two rows of micro-capillaries that are 14 μm wide, 260 μm long, and 7 μm deep and are spaced 16 μm apart. These capillaries connect the cell chamber and the two outer "source" and "sink" channels, enabling Fickian diffusion to occur throughout the device while preventing convective fluid flow into the cell chamber.23–26 The Stanford Microfluidics Foundry fabricated a 4 inch silicon mold with positive relief features using negative photoresist (SU-8) and ultraviolet light exposure through a Mylar transparency mask. After coating with chlorotrimethylsiloxane (Sigma), a 1 : 10 mixture of silicone elastomer curing agent and base (Dow Corning Sylgard© 182, Midland, MI) was poured evenly across the silicon mold. The hardened PDMS devices were removed from the mold, and inlets and outlets were punched using a 20 gauge needle. Finally, the devices were irreversibly bound to glass substrates by exposing both surfaces to oxygen plasma before placing the device onto the substrate with gentle pressure.
Two devices were mounted onto a single well within a six-well plate. Devices were washed with 70% ethanol, rinsed using Dulbecco's Phosphate-Buffered Saline (DPBS), and coated overnight with a 25 ng ml−1 solution of bovine fibronectin. BMMCs were loaded into the cell chamber at a concentration of 1 × 107 cells per ml and allowed to adhere for 30 minutes at 37 °C and 5.0% CO2. Cells that failed to adhere were washed away using migration medium (BMMC medium with 2% FCS and no KL or IL-3) and the cell chamber inlets were plugged to prevent flow in the cell chamber. Migration medium alone was introduced into the sink channel, while migration medium plus 5 μM FITC-dextran (20 kD, Sigma) and 20 ng ml−1 murine KL was introduced into the source channel via tubing connected to 100 μl Hamilton syringes. The source and sink solutions were introduced into the device at a flow rate of 0.6 ml h−1 using a syringe pump. After 1 hour, stable gradient formation was confirmed by fluorescence imaging of the FITC-dextran marker. This is defined as time = 0 for chemotaxis analysis, and the initial positions of all cells were recorded using phase contrast imaging. Following this, time-lapse images were collected every 2 minutes over a period of 3 hours using an inverted confocal microscope (Zeiss LSM510 Meta) with an environmental control chamber operating at 37 °C and 5% CO2. Control experiments omitted KL from the sink channel and were run in parallel with all test conditions. Three independent trials were performed for each condition, and approximately 120 cell tracks were recorded per trial.
To determine the KL concentration, Image J (NIH freeware) was used to measure the fluorescence intensity of the FITC-dextran tracer molecule, and the ratio of the intensity at each position in the cell chamber to the source channel was multiplied by the known initial concentration. Control experiments confirmed that the fluorescence intensity was linearly related to FITC-dextran concentration across the entire concentration range used. Each phase contrast image of the cell chamber was divided into quadrants along the length of the gradient such that quadrants 1 to 4 represent high to low KL concentrations, respectively. Thirty randomly selected cells from each quadrant were tracked manually using MTrackJ, an Image J plug-in. Cell quadrants were determined according to the starting position of the cell; thus, a cell that started in quadrant 1 would still be included in the data for quadrant 1 even if it traveled into quadrant 2 during the analysis. Cell tracks were analyzed for average speed and chemotactic index. The chemotactic index was defined as the distance the cell migrated in the direction parallel to the gradient divided by the accumulated distance the cell traveled. Therefore, perfectly random migration will have a chemotactic index of 0, perfect chemo-attraction (i.e., migration in a straight line towards higher concentrations) will have a chemotactic index of 1, and perfect chemorepulsion (i.e., migration in a straight line towards lower concentrations) will have a chemotactic index of −1. Speed was defined as the accumulated distance the cell traveled over time. Compass plots of cell tracks (with all track starting points superimposed on the origin) were created with the Chemotaxis Tool Image J plug-in (Ibidi, Martinsried, Germany).
To determine the range of KL concentrations to test in the micro-fluidic chambers, standard transwell assays were used to establish the dose–response relationships in BMMC (Fig. 1). A range of KL concentrations from 0–100 ng ml−1 was tested. There was a concentration-dependent increase in chemotaxis until maximal chemotaxis was observed at KL 8–10 ng ml−1. A decrease in chemotaxis was observed at higher concentrations. As a negative control, BMMC derived from mice deficient for WASP were simultaneously tested. WASP, the gene which is mutated in Wiskott–Aldrich syndrome, is downstream of Kit signaling, regulates cytoskeletal rearrangements, and is necessary for blood cell chemotaxis.20 In WASP-negative BMMC, there was significantly less chemotaxis than in wild-type cells (10–20% of wild-type, data not shown). As for normal BMMC, maximal chemotaxis was observed at 10 ng ml−1.
Based on the transwell results, BMMC chemotaxis was assessed in microfluidic chambers that generated a range of KL concentrations from 0 to 12 ng ml−1. The capillaries create a diffusion-enabled gradient across the chamber as the ligand moves from a source channel, which delivers KL, across the chamber to a sink channel, which contains medium alone and removes KL (Fig. 2A). To determine the cellular responses to different average concentrations of KL, images of the chambers were divided into quadrants prior to analyzing cell migration (Fig. 2B and C). The same syringe pump simultaneously drove flow into the KL gradient device as well as flow into a control device containing media alone in both the source and sink channels. With equal flow rates of the source and sink channels, no shear forces are generated within the cell chambers,24 which was confirmed by the random movement of cells observed in the control chambers (Fig. 3A).
To measure chemotaxis in response to KL, BMMC were randomly distributed in a fibronectin-coated microfluidic chamber and then placed in an incubator at 37 °C and 5.0% CO2 for approximately 30 minutes to allow cells to adhere before initiating flow in the source and sink channels to generate the KL gradient. A stable KL gradient was established within one hour and was confirmed by fluorescence imaging of a FITC-dextran tracer molecule (Fig. S1, ESI†). After gradient verification and quantification, the initial cell positions were recorded using phase contrast microscopy (designated as time = 0) and further monitored every 2 minutes for the duration of the experiment. As previously confirmed by fluorescence imaging of tracer molecules, the concentration gradient within the cell chamber remains constant once it has reached equilibrium.24,27
To analyze the dose–response relationship between chemotaxis and KL concentration, the cells were divided into quadrants according to the KL concentration they were initially located in, e.g., for a gradient of 0–12 ng ml−1, quadrant 1 (Q1) contained cells initially in a KL gradient of 9–12 ng ml−1, Q2 contained those at 6–9 ng ml−1, Q3 contained those at 3–6 ng ml−1, and Q4 contained those at 0–3 ng ml−1. The dose–response analyses showed that there were two distinct chemotactic patterns of KL-induced chemotaxis, which were concentration dependent. At KL concentrations greater than 3 ng ml−1 (Q1–Q3) cells moved towards the source channel in a dose dependent manner (Fig. 3A). Within these quadrants, the chemotactic index, i.e., directionality of migration, was related to the KL concentration, while speed of cell migration was not significantly affected (Fig. 3B and C). Unexpectedly, cells initially in the lowest KL concentrations of 0–3 ng ml−1 (Q4) underwent chemorepulsion away from the KL source channel (Fig. 3A). Accordingly, the average chemotactic index for Q4 was negative, indicating chemorepulsion at low KL concentrations (Fig. 3B). The average cell speed in Q4 was similar to that in other quadrants, although the population distribution was altered (Fig. 3C). The control chambers lacking KL showed neither forward nor reverse movements (Fig. 3A–C), indicating that there were no shear or other mechanical forces causing the observed movement in the KL chambers. Thus, the microfluidic chambers revealed a chemorepulsive response at low concentrations of KL, which has not been described previously.
We analyzed cell movements of BMMC at multiple time points from 0–150 minutes in each quadrant of KL concentration to determine if chemoattraction and chemorepulsion were equally persistent (Fig. 4A). Within Q1–Q3, after a lag phase of random migration, Kit-mediated chemoattraction persisted over the entire period of observation a total of 150 min. The duration of the lag phase appeared to be KL concentration dependent, with random migration taking place for 30 min in Q1, 60 min in Q2, and 90 min in Q3 as evidenced by both the individual cell tracks (Fig. 4A) and the population averaged chemotactic index (Fig. 4B). In contrast, cells in the lowest quadrant of KL concentration experienced no lag phase and displayed chemorepulsion at the earliest time points. This chemorepulsion persisted for a limited period of time, 90 min, after which further migration exhibited random directionality (Fig. 4). Consequently, the net movement of cells away from the source channel only increased for the first 90 minutes, after which minimal chemorepulsive movement was observed. The cells in control wells lacking a KL gradient underwent no directional chemotaxis during the entire 120 minute observation (Fig. 5). Thus, the time-dependence of Kit-mediated chemo-attraction and chemorepulsion differed.
We have previously demonstrated that WASP, a regulator of cytoskeletal structure, is a phosphorylation target of activated Kit and is essential for Kit-mediated cytoskeletal changes, Ca++ flux, and gene expression.20 Therefore, we investigated whether WASP was also necessary for both Kit-mediated chemoattraction and chemorepulsion. Observation of WASP-deficient BMMCs exposed to a KL gradient revealed that WASP-deficient cells exhibited migration that was statistically random, with chemotactic index averages close to zero (Fig. 6A). The lack of a both chemoattraction and chemorepulsion in WASP-deficient cells was similar to that observed for WT cells in the absence of KL gradient. Thus, it can be concluded that the bimodal chemotactic behavior of BMMCs, i.e., both chemoattraction and chemorepulsion, involves active processes that are dependent upon Kit-WASP signaling for cytoskeletal changes and consequent migration. To further probe the mechanism behind Kit-mediated chemotaxis, we also studied the behavior of WT BMMCs treated with pertussis toxin (PTx) in the presence of a KL gradient. Previous studies have shown that PTx can block chemotaxis through the inhibition of G-protein coupled receptors (GPCR), which would normally interact with the chemokines driving directed migration.28 PTx inhibited Kit-mediated chemotaxis of WT BMMCs in the presence of a KL gradient, and cells exhibited non-directional, random movement similar to that observed with control, WT BMMCs in absence of KL gradient, and WASP-deficient experiments (Fig. 6B). These results indicate that Kit-mediated chemotaxis is dependent upon GPCRs, which has not been reported previously.
The presence of KL induces the formation of filopodia in wild-type BMMCs (Fig. 7). However, loss of WASP signaling inhibits filopodia formation (Fig. 7C).
Our results reveal that Kit signaling generates a uniquely complex chemotactic response featuring both dose-dependent chemoattraction and chemorepulsion. Previous experiments using conventional transwell assays permitted observations of the chemoattractive response to Kit signaling, similar to those shown in Fig. 1, but they were not able to capture the chemo-repulsive response. The use of a microfluidic device, which allowed direct surveillance of individual cell movements within a stable KL gradient ranging in concentration from 0–12 ng ml−1, allowed observation of a previously unseen chemorepulsion at KL concentrations less than 3 ng ml−1. As shown by its abrogation in WASP-negative cells, the observed chemorepulsion was an active process, dependent upon the rearrangement of the actin cytoskeleton. The limited duration of Kit mediated chemo-repulsion may be due either to an intrinsic signal refractory period after low dose stimulation or to the cumulative effect of chemorepulsion moving the cells to regions of the chamber where the KL concentration was below a minimal threshold for any chemotaxis, either forward or backwards.
Previous studies of chemokine-induced chemotaxis have demonstrated both chemoattraction and chemorepulsion, but the dose–response in these cases is the converse of what we observed with Kit-mediated chemotaxis. Poznansky, et al.28 reported induction of chemorepulsion of CXCR4(+) cells, e.g., T lymphocytes, at high concentrations and chemoattraction at low concentrations of SDF1/CXCL12. Other chemorepulsive effects that depend upon a high concentration of ligand have been shown for chemokine/receptor pairs CXCR2/IL-8 (CXCL8), CXCL9/CXCR3, and CXCL10/CXCR3.29 It has been proposed that these chemorepulsive movements could impact how the responsive cells distribute themselves in relation to the ligand source; in other words, these chemorepulsive signals could help to pattern cell localization in vivo. One effect of these chemorepulsive movements is that areas of high ligand concentration may be depleted of cells expressing the cognate receptor. For example, melanoma cells that overexpress SDF1/CXCL12 escape from immune control by repulsion of tumor-specific T lymphocytes.30 Similarly, chemorepulsion induced by high levels of intrathymic SDF1 contribute to the emigration of thymocytes from the fetal thymus.30,31 Our results indicate that a converse process occurs during Kit-mediated signaling in mast cells, i.e., low levels of KL induce chemorepulsion. Such a process could be important for regulating the distribution of mast cells in the tissue such that they are more likely to be recruited to areas of high KL concentration and less likely to be present in areas of low KL expression. Thus, chemorepulsion of mast cells from areas of low KL expression may have anti-inflammatory effects. Xenotransplantation experiments have shown that both mucosal and connective tissue mast cells are derived from transplanted HSC, but with different kinetics of reconstitution and distribution in different tissues and anatomical regions.32 The bimodal response to KL signal intensity might be a mechanism to explain such differences: tissues expressing low level Kit ligand may actively repel mast cell progenitors.
In addition to the KL gradient concentration profile, the intensity of Kit signaling might also be modulated by receptor density. For example, Kit expression in BMMC is uniformly high,20 but much greater variation is observed among hematopoietic progenitors.33 Experiments with other Kit-expressing cell types will be important in determining whether the bimodal chemoattractive and chemorepellent responses observed in BMMC are generalizable and how KL concentration affects cells with differing receptor density.
The mechanisms by which different concentrations of chemotactic molecules can result in differential chemoattractive and repulsive signals are not well understood. In SDF1-mediated axonal guidance, the direction of axonal outgrowth is controlled by differential contributions of secondary signals downstream of phospholipase C (PLC).34 Activation of protein kinase C (PKC) results in repulsion of the axonal growth cone, while activation of the inositol triphosphate (InsP3) receptor (InsP3R) resulted in attractive turning. In neutrophils, the balance of levels of InsP3 generated by phosphoinositol-3 kinase (PI3K) vs. phosphatidic acid generated by catalysis of InsP3 by phospholipase D (PLD), controls the direction of neutrophil signaling in response to bacterial chemoattractants.35 PLC-gamma and PLD are prominent targets of Kit signaling, and both PKC activation and InsP3 generation are known to occur after Kit stimulation.13,36,37 The mechanism, by which the intensity of Kit signals could cause differential levels of activation of PKC, PLC-gamma, or PLD, is not known. We have previously described topological changes in the distribution of Kit, the PI3K p85 regulatory subunit, and the phosphatase and tensin homologue (PTEN) after Kit stimulation.38 These experiments were performed with cells suspended in high concentrations of KL, which were chosen to maximize Kit responses. Lower intensity of Kit signaling, e.g., those occurring at the chemorepulsive concentrations described in this paper, may alter the relative activation of PI3K, PTEN, PKC, PLC-gamma or PLD, and thereby alter the balance of phospholipids that regulate the direction of chemotaxis.
In addition to the discovery of dose-dependent chemoattraction and chemorepulsion, we have also established that both chemorepulsion and chemoattraction depend on activation of a heterotrimeric G-protein dependent pathway, since pre-treating cells with PTx blocked any directed migration. Although previous studies of Kit-mediated chemotaxis have shown dependence on Fyn phosphorylation, PTEN, and Jak-Stat signaling,10 they have never before shown dependence on a G-protein. Activation of a G-protein might occur directly downstream of either Kit tyrosine kinase activity or via a secondary mediator, such as Src or Jak-Stat proteins. Previous evidence has shown that cross-receptor interactions between Kit and type I cytokine receptors, e.g., the Epo or interleukin-7 receptors (EpoR, IL-7R) are essential downstream effectors of Kit signaling;39,40 the type I cytokine receptor is tyrosine phosphorylated and activated by Kit in these interactions. For example, Kit-mediated support of erythroid differentiation is dependent on the expression of the Epo receptor. An alternative mechanism for the dependence of Kit chemotaxis on a G-protein pathway could be analogous cross-receptor interaction of Kit with a chemokine receptor, which in turn acts as a guanine exchange factor for a heterotrimeric G-protein. Such a mechanism would be the converse of a previously described interaction in which a leukotriene receptor activates Kit.41
The studies described here emphasize the importance of direct observation at the cellular level for cells undergoing chemotaxis within a chemical gradient. Standard transwell assays, e.g., those shown in Fig. 1, and similar assays that are based on counting the total number of cells that have reached a designated target produce a binary output, i.e. either the cells have moved to the target or they have not. These assays do not provide sufficient information to detect complex chemotactic responses, such as those described here. In a transwell assay the chemo-repelled cells at the lower ligand concentrations would have simply been scored as unresponsive to the chemo-attractant. In contrast, the microfluidic device described for these experiments provides a trove of information including migration speed, directionality, and persistence of individual cells over a range of concentrations as a function of time. In addition, the microfluidic device can maintain a stable linear chemical gradient for long periods of time (well over 12 hours),27 contrary to transwell assays, which create a time-dependent gradient. This enables exact specification and quantification of the chemokine gradient profile to identify concentration-dependent effects. The ability to directly observe cells over time in these microfluidic devices will prove an invaluable asset to the discovery and deconstruction of these complex chemotactic responses of BMMC and other Kit+ cell types, e.g. hematopoietic progenitor cells.
In conclusion, the microfluidic chambers used for these experiments allowed single-cell analysis of cell migration in response to a KL gradient. These experiments lead to the discovery of a complex chemotactic response that involves both chemoattraction and chemorepulsion. The novelty of the response observed lies in both its dose response, that chemorepulsion occurs at low KL concentrations and attraction at high concentrations, as well as its dependence on G-proteins, which had not been previously reported in Kit signaling. These observations would not have been possible using traditional chemotaxis assays, such as transwells, indicating the need for single-cell analysis techniques to study the complexities of cell migration.
We present a microfluidic device that enabled us to perform a single-cell analysis of the chemotactic response of bone-marrow derived mast cells (BMMCs) to a linear gradient of Kit ligand (KL). This technological innovation led us to the discovery of a complex time-dependent chemotactic response that involves chemoattraction at high concentrations of KL and chemorepulsion at low concentrations, which may help explain how mast cells pattern themselves within the body during maturation. Further experimentation using pertussis toxin revealed that the response observed must involve the tyrosine kinase receptor, c-kit, as well as a G-protein-couple receptor. These biological insights would not have been possible without integrating a microfluidic device engineered by a materials science lab and techniques developed in a hematology lab.
Supported by NIH grant CA049605 (KW), the Lucile Packard Foundation for Children's Health (KW), and NIH grant DP2-OD006477-01 (SH). The early, consistent support of Benja-min and Patty Anixter, and Stephen and the late Harriet Zelencik is gratefully acknowledged. M. Manchandia was a recipient of a Stanford Undergraduate Advising and Research grant. CN was a Rose Hills Researcher of the Office of the Vice Provost of Undergraduate Education at Stanford University. MF was a recipient of the Diversifying Academia, Recruiting Excellence fellowship from the Office of the Vice Provost of Graduate Education at Stanford University and the National Science Foundation Graduate Research Fellowship.
†Electronic supplementary information (ESI) available. See DOI: 10.1039/c3ib40025e
PubMed Central Canada is a service of the Canadian Institutes of Health Research (CIHR) working in partnership with the National Research Council's Canada Institute for Scientific and Technical Information in cooperation with the National Center for Biotechnology Information at the U.S. National Library of Medicine(NCBI/NLM). It includes content provided to the PubMed Central International archive by participating publishers.


text:
Classic debuts at Strayhorn
After a breather last Friday, the Tunica Academy Blue Devils return to the field tonight (Friday) for its first football match-up with Strayhorn High School. Kickoff is 7 p.m. at Strayhorn.
Organizers of the inaugural County Line Classic hope it won't be the last meeting of the friendly rivals.
Latest News
Town, county wait to hear judge's ruling After a half a day...
Board splits over Chandler evaluation On June 10, the Board...
Kirkland tapped for Coaches' Hall of Fame The winningest boys basketball...
Hercules Hampstead Stage players...
New airman proves 'good' comes from Tunica "When the world...
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8


text:
Ratings based on 39 Verified Reviews
They paid and stayed. We double-checked.
4.7 Room cleanliness
4.4 Service & staff
4.7 Room comfort
4.7 Hotel condition
They paid and stayed.
We double-checked.
We double-checked.
for Everyoneby A verified traveler
Awesome!!Posted Apr 7, 2015
Watching zebras and ostrich and cattle from our balcony was a truly amazing experience!!! It was a very long walk to our room but in the end it was worth it!!!
for Familiesby A verified traveler
An unforgettable stayPosted Mar 2, 2015 on Hotels
A truly fantastic stay. Not only are you close to all the Disney Theme Parks, the rooms are also big and beautifully furnished and you can't beat the views of Zebra, Giraffe, Kudu etc. walking past your room all day.
for Familiesby Dr John from New Jersey
Incredible!Posted Nov 14, 2014 on Hotels
This Hotel was phenomenal. Our girls thoroughly enjoyed themselves and all the amenities within the property as well as the animal watching.
for Everyoneby A verified traveler
2 Night StayPosted Oct 6, 2014
very well kept room, no stress check in after doing it all with My Disney Experience. Disney matched my requests for room type perfectly.
Recommendedby Mccue from Miami
HorriblePosted Aug 10, 2014 on Hotels
I will never ever reserved with hotel.com again Nothing particular with the hotel . They didn't transfere the reservation to the hotel so no room upon arrival ...... They destroy my Disney experience
for Everyoneby A verified traveler from Nottingham
A resort in itselfPosted Aug 1, 2014
Pros: So much to see and do in this resort. Luxury bathrooms in the villas and awesome savannah views. Car park sits underneath the accommodations brill
Cons: Cannot think of anything to improve this resort.
Location: Everything is easily accessible with the disney transport bus. No problems using a car as you get a parking zone with every villa.
We stayed in a 1 bedroom villa 2 adults 16 year son 11 year daughter. Fantastic - the room the savannah, the place is huge and you can enjoy so much of the resort itself without including the theme parks. The pool at Kidani Village is fabulous, never crowded, loads of water play areas and a slide. Campfire fun at night is brilliant, the restaurants are fab. The 1 bed savannah view is well worth the extra money as you can enjoy viewing the animals over breakfast lunch or dinner and we used the balcony loads, even though we are hardened theme park goers. We used disney buses and they go direct to their destination which is better than other disney resorts, although it takes longer to get to disney attractions. A really luxurious villa definitely recommended to suit everyone.
for Everyoneby A verified traveler
Grandson's First VisitPosted Jul 23, 2014
Pros: 3 bathrooms
Cons: Would be nice if they had a counter service restaurant for breakfast.
My wife and I, our 2 daughters and our grandson stayed for 5 nights at Kidani Village and it was wonderful as usual. We had a 2 bedroom, 3 unit that was perfect. Getting to the parks takes a little longer but the stay was worth it.
for Everyoneby A verified traveler
Expensive, but worth it.Posted Jul 13, 2014
Highest quality furnishings and very roomy 1 bedroom villa. Savannah views lived up to billing. Everything about room and experience was positive. Only "complaint" is sheer magnitude of lodge ... it was literally a 10-15 minute walk from lobby to our room (we had 2nd furthest possible room from lobby). Minor inconvenience, though
for Everyoneby yohann
Posted May 6, 2014
Pros: I like comfort !!!
Nice experience for family, room is big and view is nice !!
for Familiesby ROB from KANSAS
awesome placePosted Mar 25, 2014 on Hotels
Disney just does it right. The resort is new and extremely nice. Get the Savannah view if you can. In my opinion, it is worth it. The animal come in every night about 5:30 and really is an incredible view. The pool area is very nice and it has a slide that the kids really enjoyed. It was very relaxing to come back after a tired and hectic day at the parks and just sit by the pool. They have movies under the stars every night about 8:30 as well. I Highly recommend this resort.


text: Remembering Doc Watson With 'Tennessee Stud' May 30, 2012 Legendary folk singer and guitarist Doc Watson died on Tuesday, at the age of 89. Long considered one of America's greatest musicians, Watson was blind from the age of one, and taught himself to play music. NPR's Neal Conan remembers the life and career of Doc Watson with a song: "Tennessee Stud." Listen Loading… 2:51 Playlist Download Embed Embed Close embed modal <iframe src="http://www.npr.org/player/embed/154000832/154000831" width="100%" height="290" frameborder="0" scrolling="no">


text:
X-Files-Season 7 (1999)
Starring: Gillian Anderson David Duchovny
Learn more about X-Files-Season 7:
Format: DVD
Manufacturer: Foxvideo
Sku: 202439471
Category Keywords: Alien Abductions Alien Encounters Alien Life Forms Aliens Conspiracies FBI Agents Illness Science-Fiction Series Television/TV Series
Rating: NR
See more in Television
"A Time of Closure, A Time of New Beginnings. "
|Now you can own the entire seventh season of The X-Files. All 22 classic episodes from David Duchovny's last full season as Agent Fox Mulder are available for the first time in this exclusive 6-disc collector's edition. From Scully discovering the alien spacecraft in "The Sixth Extinction" and Mulder finally learning the truth about his sister in "Closure," to Mulder's own disappearance and Scully's miraculous pregnancy in "Requiem," these Season Seven episodes are a must for every X-Files fan!
Editor's NoteScience fiction and conspiracy are in ample supply in this seventh season of the popular Fox television series, THE X-FILES. FBI Agents Mulder (David Duchovny) and Scully (Gillian Anderson) continue to investigate unexplained events while battling their own demons. Fans of the show will also be in heaven with the number of in-jokes that occur throughout this season. Among the classic episodes is "Hollywood A.D." Directed and written by Duchovny, the episode surrounds a TV-movie with celebrities Garry Shandling and Tea Leoni (Duchovny's real-life mate) playing the agents.
Cast & Crew
|David Duchovny
|Gillian Anderson
|Mitch Pileggi
|Chris Carter - Created By
|Frank Spotnitz - Producer
|Frank Spotnitz - Writer
|James Wong - Director
|Mark Snow - Original Music By
|Vince Gilligan. et al. - ScreenwriterScreenwriterScreenwriter
Emmy (2000)
|Bill Millar. et al., Winner, Outstanding Special Visual Effects
|Cheri Montesanto-Medcalf, Winner, Outstanding Makeup for a Series
|Mark Snow , Nominee, Outstanding Music Composition


text:
Joan Jett sues Hot Topic over Blackheart trademark
Story | Jun 13, 2013 | 12:43 PM
Joan Jett sues Hot Topic over Blackheart trademark
Story | Jun 13, 2013 | 12:43 PM
Retailers report soft May sales figures
Story | Jun 3, 2010
The nationâs consumer spending recovery hit a snag in May as retailers reported soft results that hinted at what could be a tough summer for spending. On Thursday, major chain stores posted a 2.5% year-over-year increase in May sales, according...
Few women in executive ranks in California, UC Davis survey finds
Story | Dec 2, 2010
Women hold fewer than 10% of all top executive and board of directors positions within Californiaâs 400 largest companies, according to a study released by UC Davis. Though several L.A. companies made the top 25 list of firms with the...
L.A.'s John Eshaya to design collections for Hot Topic and Torrid
Story | Dec 27, 2010
John Eshaya, founder of the contemporary Jet Clothing brand and former vice president of women's wear at Ron Herman, has signed on as the first name-brand designer to create collections for teen-focused retailer Hot Topic and its plus-size spin-off...


text:
Welcome Hearth.com Guests and Visitors - Please enjoy our forums!
Hearth.com GOLD Sponsors who help bring the site content to you:
Hearthstone Soapstone and Cast-Iron stoves( Wood, Gas or Pellet Stoves and Inserts)
Are the echo choice heatilator pellet stoves made by quadrafire?
Post in 'The Pellet Mill - Pellet and Multifuel Stoves' started by Jarhead1229, Nov 12, 2011.
- Thread Status:
- Not open for further replies.
- Thread Status:
- Not open for further replies.


text:
This is a very rare and special vehicle. It was built as a factory "Brass Hat" show car and not even sold to a... More
Gateway Classic Cars is proud to offer a very unique 1966 Plymouth Valiant Signet convertible. Plymouth introduced the Valiant for the 1960 model... More
Plymouth 1966 Valiant 200, good condition, red exterior and interior, very little rust and no rips on interior, $2,000. 631-472-2594.... More


text:
What's News: World-Wide
Obama's approval ratings fell to their lowest level. A Wall Street Journal/NBC poll found 44% of Americans approve of his job as president; over half for the first time disapproved. Over 70% said the economy hasn't hit bottom. By 44% to 40%, Americans now say they are more likely to vote Republican in 2012.
Obama called for job creation and Romney sought to stake out moderate ground in Labor Day electioneering.
U.S. military and spy officials are seeking more authority for covert operations to thwart Iran's sway in Iraq.
Iran has broadened a crackdown on activists in a bid to pre-empt protests as regional revolts gained momentum.
Libyan rebels closed in around the pro-Gadhafi stronghold of Bani Walid but held back on a final assault.
Pakistani agents working with the CIA arrested an al Qaeda leader who sought to hit U.S. economic interests.
Syrian soldiers raided homes and made arrests in a hunt for an official who defected from Assad's regime.
U.S. authorities are probing the release of an Arizona man accused of supplying grenades to a Mexican drug cartel.
Turkey said it won't change economic relations with Israel "for now" as a rift between the two nations deepened.
Japan braced for more rain and floods as the typhoon death toll climbed to 34.
Torrential rains from Tropical Storm Lee's remnants swept through the South.
A wildfire destroyed nearly 500 homes and forced evacuations near Austin, Texas.


text:
Do Online Relationships Work?
Through out the generations, love has found its way to blossom no matter what the medium is. There used to be a time when marriages were arranged by the families. In the earlier times, the boys and girls met in any social event and the courtship started that used to end in sweet relationships. Courtship has changed its forms with time.
Dyslexia: Signs or Symptoms to Take Into Consideration
Occasionally, someone with dyslexia can reach their adult years without even realizing she or he has the situation.
I Like Hollie's Quotes
I have found a treasure trove of sayings!
Coaching or Comparing?
Negative tactics almost always produce negative results.
Yesterday's Slang Not So Bad!
It was often colorful………….
Time to be Courageous
Try to be more courageous.
Ways to Increase Your Vocabulary: Participating in The Magic of Words
Increasing your vocabulary is like learning a new language. It will open doors and introduce you to opportunity.
Five Words That Destroy The Relationship
The words have a powerful effect – can hurt you, help you, inspire you and answers some purpose. Learning marital language requires time and patience, but some of the words you keep repeating can cause irreparable damage.
Phrases You Can Learn in Japanese From Watching Anime
The typical phrases and words people can learn from watching anime.
Don't Tell Me What to Do
Just some thoughts I wanted to share with you.


text:
VIEWS: 28 PAGES: 4 CATEGORY: Business POSTED ON: 3/14/2010 Public Domain
Open Letter on PBS and FTA
To the Honourable John Howard MP, Prime Minister of Australia and The Honourable Mark Latham MP, Leader of the Opposition, 27 September 2004 Safeguards to ensure Protection of the Pharmaceutical Benefits Scheme from Provisions in the Australia-US Free Trade Agreement We the undersigned, urge bipartisan cooperation to ensure that prior to formal bilateral ratification of the Australia US Free Trade Agreement (AUSFTA) in late October 2004, additional safeguards are attached to the treaty to clarify ambiguities in the text that may undermine the integrity and basic framework of the Pharmaceutical Benefits Scheme (PBS). The course of negotiations and subsequent public debate has highlighted that the US1 and Australia2 may have significantly different interpretations of key articles and provisions in the AUSFTA text related to the PBS. A unilateral interpretative declaration would put on record Australia's interpretation of these ambiguities, which could play a vital role to protect the PBS in the event of a dispute under the AUSFTA. Additionally, in light of recent comments by the Deputy US Trade Representative 3 indicating intent to use the joint AUSFTA implementation committees and working groups for ongoing changes to the PBS, we urge full transparency of these processes and that the PBS cost-effectiveness pricing system be protected from the scope of these committees. These measures could be taken in accordance with the rules of international law and should not delay ratification of the AUSFTA. Footnotes: 1 'The FTA makes suitable progress on addressing Australia's drug price controls' (Senator Kyl, Congressional Record: July 15, 2004 (Senate), p.S8208). 2 'We have secured absolute protection of the Pharmaceutical Benefits Scheme. Yes, some of the American drug companies did try and get in under the radar on that subject, but we didn't agree' (PM John Howard, Archival footage, 29/07/04 quoted in Holmes, A Bitter Pill?, 4 Corners, ABC, 02/08/2004). 3 'Crucially, the FTA also establishes a Medicines Working Group that will provide a forum for ongoing dialogue on Australia's system of comparing generics to innovative medicines. We urge Congress to act quickly to approve the U.S.-Australia FTA, so that we may begin implementing agreed provisions and working toward further reform. (Testimony of Deputy U.S. Trade Representative Josette Sheeran Shiner before the Committee on Finance, Subcommittees on Health Care and International Trade, United States Senate, April 27, 2004. p5) Open letter on PBS and AUSFTA 27 January 2004 1/4 While the extent of the increased risk to the PBS is subject to debate, trade agreements should not be the forum to drive reforms to domestic health programs such as the PBS. We note these sentiments are shared by many US Representatives.4 We therefore urge prior to the exchange of notes in October 2004, bipartisan commitment to the establishment of such safeguards that clarify Australia's understanding that the PBS system is protected in the AUSFTA. Yours Sincerely, The undersigned (ORGANISATIONS) Professor Peter Sainsbury Morrie Mifsud, JP President, on behalf of the State President Combined Pensioners & Public Health Association of Australia Superannuants Association of NSW Inc Andrew McCallum President, on behalf of the Denele Crozier Australian Council of Social Service (ACOSS) Executive Officer on behalf of Women's Health NSW Peter Kell Chief Executive Officer, on behalf of the Dr Patricia Ranald Australian Consumer Association (ACA) On behalf of the Australian Fair Trade and Investment Network Professor Gwen Gray Deputy Convenor, on behalf of the Australian Women's Health Network 4 'Domestic healthcare policy should not be decided in trade agreements–It is wrong for us to interfere with another country's domestic health policy, particularly when it comes to the affordability of medicine which is an equally sensitive issue here in the United States' (Representative Henry Waxman speaking during US Congressional debate on the AUSFTA, US Congressional Record- Extensions of Remarks, 16 July 2004, p.E1397-E1398). Open letter on PBS and AUSFTA 27 January 2004 2/4 (INDIVIDUAL SIGNATORIES) Professor Paul Gatenby Frank Fenner, AC, CMG, MBE, FRS, FAA, FRACP, FRCP. Dean John Curtin School of Medical Research Medical School The Australian National University Emeritus Professor Bob Douglas Julian Disney Founding Director (1989-2001) Director, Social Justice Project University of NSW National Centre for Epidemiology and Population Health Former President ACOSS and World President of the Australian National University International Council on Social Welfare (ICSW) Dr. Ron Wells, AM., M.D., F.R.A.C.P. Former Deputy Director-General, Commonwealth Department of Health Professor Michael Dodson Former Aboriginal and Torres Strait Islander Social Justice Commissioner Professor and Chairperson Institute for Indigenous Australia and Australian National University Richard Refshauge SC Director of Public Prosecutions Australian Capital Territory Professor Simon Chapman School of Public Health University of Sydney Professor David Henry Professor of Clinical Pharmacology University of Newcastle Former Chair, Economic Sub-Committee of the Pharmaceutical Benefits Advisory Committee Professor Wayne Hall Ph.D. Professor and Director Officer of Public Policy and Ethics Professor Wayne Smith Institute for Molecular Bioscience School of Medical Practice & Population Health University of Queensland University of Newcastle Open letter on PBS and AUSFTA 27 January 2004 3/4 (INDIVIDUAL SIGNATORIES CONTINUED) Dr Thomas Alured BA LLB (Hons) B Med. PhD Pieta Laut Dr Buddhima Lokuge Senior Lecturer, ANU Medical School Executive Medical School and Director Lecturer, ANU Law Faculty Public Health Association of Australia Regulatory Institutions network Australian National University Dr Peter R Mansfield, Director, Healthy Skepticism Inc General Practitioner and Research Fellow Department of General Practice Dr Buddhima Lokuge Pieta Laut Executive Director, Public Health University of Adelaide Health Policy Consultant Association of Australia Visiting Fellow, Medical School and Regulatory Institutions network Australian National University Jennifer Clarke Lecturer, ANU Law Faculty Dorothy Broom, PhD, FASSA, AM Senior Fellow National Centre for Epidemiology & Population Health Dr Peter R Mansfield, The Australian National University Director, Healthy Skepticism Inc General Practitioner and Research Fellow Catherine Curtain Department of General Practice Law School, Melbourne University University of Adelaide Dr Angela Taft Research Fellow Mother and Child Health Research, Martyn Goddard La Trobe University Former member of the Former Vice-President (Policy) Pharmaceutical Benefits Advisory Committee, Public Health Association of Australia Former member of the Australian National Council on AIDS Open letter on PBS and AUSFTA 27 January 2004 4/4
Pages to are hidden for
"Open Letter on PBS and FTA"Please download to view full document