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83-14389 - Raspberry Pi Breakout Cable Kit with GPIO Cable
Now that you've finally got your hands on a Raspberry Pi® , you're probably itching to make some fun embedded computer projects with it. What you need is an add on prototyping Pi Cobbler from Adafruit, which can break out all those tasty power, GPIO, I2C and S...
Mfr Name: Adafruit Industries
Mfr Number: 914
See all items in Project Labs
83-16422 - Pibow Pi Coupe Enclosure for Raspberry Pi™ B+
Pibow Coupé is a slim, hackable and attractive case crafted out of four unique smoked layers with a transparent top and base that leave your beautiful Pi visible inside. Each layer is laser-cut from colorful high-quality cast acrylic and once stacked they securely...
Mfr Name: Pimoroni
Mfr Number: PIM010
See all items in Raspberry Pi
21-14610 - 6" PCB Reference Ruler
The first time you soldered up a surface mount component you may have been surprised "these are really small parts!" and there's dozens of different names too! QFN, TDFN, SOIC, SOP, J-Lead, what do they mean and how can you tell how big they are? Now you can have ...
Mfr Number: 1554
See all items in Ruler
83-14384 - Raspberry Pi Prototyping Plate Kit
Now that you've finally got your hands on a Raspberry Pi® , you're probably itching to make some fun embedded computer projects with it. What you need is an add on prototyping Pi Plate from Adafruit, which can snap onto the Pi PCB (and is removable later if you wi...
Mfr Number: 801
See all items in Breadboard Kits
555-4051 - Professional Home/Studio Stereo Preamplifier
May be used on a tabletop or mounted in a rack, making it suitable for professional or high end home use. Front panel includes an input for microphone, with level control. Rotary controls adjust volume, loudness, bass, treble and midrange. Other features include h...
Mfr Name: Pyramid
Mfr Number: PR2500
See all items in Preamplifiers
21-13508 - TD Series Desktop Barcode and Label Printer with Network Capability
The TD-4100N desktop unit provide a complete barcode software and label printing solution that prints on most medium/high sensitivity direct thermal media in high quality 300dpi resolution, and comes ready with everything you need to start printing including the U...
Mfr Name: Brother Mobile
Mfr Number: TD4100N
See all items in Electronic Labelers
82-14100 - Stopper® II with Horn and Relay Flush Mount
This unique and patented device has been helping stop false fire alarms around the world for almost 30 years, without restricting legitimate alarms. When lifted to gain access to the actual alarm, a piercing 95 or 105 dB warning horn sounds.
Mfr Name: Safety Technology International
Mfr Number: STI-1100RC
See all items in Theft Prevention
28-11118 - Interchangeable DC Plug Kit for AC Adapter
Make sure you have the DC plug you need, when you need it, with this wide assortment of interchangeable DC plugs.
Mfr Name: Distributed By MCM
Mfr Number: MW X-15
See all items in DC Adapters
555-13862 - 8 Channel 32 Ft Xlr Cable Snake
Eight Channel Cable Shake - 32'
Ideal for permanent installation, stage rigs, and any PA system, this heavy-duty cable snake features a rugged metal stage box and quality 3 pin XLR connectors, and keeps microphone and instrument cable runs organized between the s...
Mfr Name: Stellar Labs
Mfr Number: 555-13862
See all items in Cables
82-13315 - Lock N a Box Magnetic Door Access Control System 600LB Holding Capacity
Ideal for indoor use in any commercial environment, this access control system consists of a 600lb electromagnetic lock, backlit digital keypad, push to exit button, and a 24V power supply.
Mfr Name: Alarm Controls
Mfr Number: LNB-6
See all items in Magnetic Locks
555-13900 - 16 Send / 4 Return 49 Ft Xlr Cable Snake
16-Channel Cable Snake with Four Returns - 49'
Ideal for permanent installation, stage rigs, and any PA system, this heavy-duty cable snake features a rugged metal stage box and quality 3 pin XLR connectors, and keeps microphone and instrument cable runs organize...
Mfr Number: 555-13900
82-18220 - Complete 8-Camera Digital Surveillance 960H DVR Camera Kit
The Defender 960H Network DVR Kit is an all-in-one surveillance solution with advanced widescreen high resolution recording and 700TVL bullet cameras featuring powerful night vision up to 60ft in total darkness.
Internal hard drive must be ordered separately, se...
Mfr Name: Defender Security
Mfr Number: 82-18220
See all items in 8 Camera DVR Kits
830-2520 - Rhino 4200 Labeler
Compact, time-saving labeling for on-the-go pros.
Mfr Name: Rhino Products
Mfr Number: 1801611
51-1100 - Pyle Blue Label 4'' 2 Way Speaker Pair - 180W Max
4'' 180 Watt Two-Way Speakers (pair)
Pyle's Blue Label series speakers don't just sound great – they look great, too. They're great replacements for your vehicle's factory speakers – you'll hear the difference immediately. Included is a pair of round 4" two-way ...
Mfr Name: Pyle
Mfr Number: PL42BL
See all items in Piezo Horn Tweeters
21-13471 - P-Touch Edge™ Industrial Labeling Tool
The PT-E100 is a compact, handheld labeler designed to help contractors be more productive by labeling quickly and efficiently. Standalone system with display, keyboard and cutter lets you type, print and cut the perfect label.
Mfr Number: PTE100
Overall, my MCM experience was great! They delivered fast and as promised. Great product, great price!


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Suffolk County
Suspect In Shooting Of Suffolk County Cop Pleads Not Guilty
Officials say Officer Mark Collins was shot just before midnight Wednesday while confronting a suspect who fled a traffic stop in Huntington Station.
Police Investigating 2 Separate Shootings In Suffolk County
Police are asking anyone with information regarding the two shootings to call Crime Stoppers at 1-800-220-TIPS.
3 Injured In Hauppauge Crash, Including Stony Brook Fire Chief
Police said a man driving with a passenger was heading south on Route 111 when their car struck the SUV belonging to the Stony Brook fire chief.
L.I. Man Sentenced In Wrong-Way Crash After Pleading Guilty To DWI
A Long Island man has been sentenced to 2 1/2 to five years in prison in connection with a wrong-way crash that seriously injured a married couple last year.
LI Lawmaker Proposes Bill To Halt Gaming Facilities In Nassau, Suffolk Counties
After the Westbury community came together and fought the plan to bring a casino to their neighborhood, a lawmaker has proposed a bill that would halt gaming facilities in Nassau and Suffolk counties.
Long Island Is Most Expensive Place To Live In U.S., Website Says
Stamford-Norwalk ranked third, Westchester County was fifth, and New York City eighth.
State Lawmaker Proposes Legislation To Block OTB Gaming Parlors On Long Island
A state lawmaker is introducing new legislation to prohibit the Off-Track Betting Corporation from building gambling parlors on Long Island.
Suffolk Co. Police Recruit Pool Smaller This Year Than Years Past
One of the largest and highest paid police departments in the nation is looking for more than a few good men and women.
Officials Urge Caution As Tri-State Area Is Socked With Snow, Bitter Cold This Weekend
The extreme cold will last throughout the weekend with Sunday being the worse for some areas where it will feel like below zero.
LI Parents Ask School Districts To Enforce Stricter Safety Regulations For Contact Sports
There are calls being made on Long Island for new rules to protect student athletes from unsafe equipment.
Schumer Calls On FAA To Expedite Approval For Part Of Mastic-Shirley Sewer Project
Suffolk officials are waiting on the FAA to move forward with a massive sewer project.
Police: L.I. Man Burglarizes Home, Kills Cat After Crashing Car
A Suffolk County man facing a series of charges in connection with a crime spree that started with a car crash and ended in the death of a cat is due in court Thursday, Nassau County police said.
Suffolk County Continues To Dig Out Following Massive Storm
Snow is still piled as high as street signs on snowy Long Island as residents continue to dig out from the storm.
Suffolk County Digging Out From Storm That Dumped 2 Feet Of Snow
Gov. Andrew Cuomo said Tuesday that about 500 pieces of equipment, plows, dump trucks, front end loaders, are on their way to Suffolk County from New York City and the mid-Hudson Valley.
Pumps Stolen From 3 Out-Of-Business Gas Stations On Long Island
Police on Monday were trying to find the thieves who stole seven gas pumps from several shuttered service stations on Long Island.

subset: commoncrawl


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Nyack College Arts and Sciences News
Dr. Poston Publishes Chapter on Muslim Americans
Posted by Fernando.Arzola on Thursday June 5, 2014
Dr. Larry Poston, Professor of Religion, publishes "Islamic Da'wah and Christian Evangelism: The Problems and Prospects of Mutual Exclusivity" in Muslim American Life: Reflections and Perspectives (University of Indianapolis Press), 2014).
This text is edited by Dr. Iqbal J. Unus, Visiting Fellow of the Prince Alwaleed bin Talal Center for Muslim-Christian Understanding in the School of Foreign Service at Georgetown University.
For more information:
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Fit4Life Body Transformation Challenge
It is a 12 week programme designed to improve not only one's health but one's overall fitness levels and physique. It is centred around a total "physique and lifestyle" transformation. During the 12 week period, participants will attend 1 hour group training sessions two, three or four times a week. The training sessions are a combination of cardio and weight training exercises aimed at increasing fitness levels and improving muscle strength and tone. The sessions are run by a qualified personal trainer who monitors correct form and encourages participants to ensure that the optimum results are achieved. It really is a fun, exciting and effective way to get into the BEST shape of your life.
Participant's goals may be to get Fit4Life; lose weight, stay in shape, improve their lifestyle, become more active etc. The benefits of the challenge to the participant are numerous (just to mention a few):
~ Improved health (improves cholesterol levels, lowers high blood pressure, lowers risk of diabetes)
~ Improved fitness (keeps your body fit and able)
~ Increased self confidence and makes you feel happier
~ Better performance and productivity both at home and at work
~ Cope better with stress and alleviates anxiety
~ Reduces feelings of depression and lessens fatigue
~ Healthy body = a healthy mind (boosts mental health)
Please feel free to browse our past challenge gallery to see what the Fit4Life Challenge is all about.
You only have one body and it is up to you to make it LAST!
The Fit4Life Chalenge is a 12 week program designed and centred on a total lifestyle transformation, giving you the tools, the training and support you need to achieve your goals!
Fit4Life Challenge11 starts on the 25th of MAY 2015
Prices are as follows:
R2600 - 2 group training sessions per week (24 sessions)
R3200 - 3 group training sessions per week (36 sessions)
R3500 - 4 group training sessions per week (48 sessions)
We have launched a new LOYALTY PROGRAM and REWARDS PROGRAM!
We also have payment plans available!!
Please note we do offer Express Challenges as well - 8 week and 6 week!!!
So email email@example.com for more details!
If you get FOUR people to sign up - you TRAIN FOR FREE!!!
So stop saying tomorrow and register today!!
Fit4Life now offer an Express Challenge.
Fit4Life 6 week express challenge starts: 06 July 2015
6 week challenge price:
R1400 - 2 group training sessions a week
R1750 - 3 group traning sessions a week
R1900 - 4 group training sessions week
e-mail email@example.com for more details.
Join Fit4Life and become apart of a healthy and fit family!
You have one body...........MAKE IT LAST!!


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Just saw The Tricky Part starring Kevin Ford at Byrdie's Gallery on St. Claude. Controversial but important material & convincing portrayal. 1447 days ago
Posts In 5/2011
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Top Definition
To be in a state of advanced lovesickness, for no person in particular, akin to horniness, but without the sexual connotation. Desiress of affection and love, though without a specific person in mind.
Sally was so slargelterfied that she married the first man who told her she was beautiful.
by Caitlin February 25, 2004


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Franconia, New Hampshire Hotels & Resorts - On a winding country road, just past Robert Frosts summer cottage. The Franconia Inn has been welcoming guests since 1886. Elegant American cuisine highlights the Inns quiet country sophistication. On location, year round, recreational activities.
Greenfield, New Hampshire Bed & Breakfasts - SLEEP TOGETHER IN VICTORIAN SPLENDOR--FULL BREAKFAST PARTY WITH CRYSTAL CHINA AND MOZART only 90 Min. from Boston and 4 1/2 hrs from New York...tour every room /two pictures per room at
Chocorua, New Hampshire Bed & Breakfasts - Award-winning ten room inn on 15 wooded acres along the Chocorua River. Enjoy scrumptious multi-course breakfasts on a deck overlooking the river. Surrounded by mountains, river and lakes to hike, canoe or snowshoe.
Gilmanton Iron Works, New Hampshire Vacation Rentals - Four Bedroom Lake Front home on Crystal Lake, in Historic Gilmanton Iron Works
Harts Location, New Hampshire Bed & Breakfasts - A comfortably elegant 1860's mansion on 100 forested acres. Seven spacious rooms, six suites and three cottages, most with woodburning fireplaces, some with Jacuzzis. Romantic 5-course dinner, bountiful breakfasts.
Bartlett, New Hampshire Vacation Rentals - Large, comfortable townhouse in the heart of the White Mountains. Features 3 bedrooms, 2 baths plus a jacuzzi, and a heated pool in the summer. Townhouse offers all the amenities of home to make this ...
Lincoln, New Hampshire Vacation Rentals - LIMITED TO 5 PEOPLE: Escape to the White Mountains for a delightful vacation or a fun get-away weekend. This large, charming non-smoking condo with spectacular mountain views is located in the heart o...
Bretton Woods, New Hampshire Vacation Rentals - Gorgeous ski chalet, ski on and ski off at Bretton Woods near Mt. Washington. Every luxury: gourmet kitchen, plasma tv's, pool table, fine linens, 2 fireplaces with firewood provided, full equipped.
Wolfeboro, New Hampshire Bed & Breakfasts - Stay on a traditional New England farm in the Historic District of Wolfeboro, NH. The Guest House at Mustard Seed Farm is a charming alternative to the Inns and Resorts while visiting the Lakes Region and Mountains.
Sugar Hill, New Hampshire Bed & Breakfasts - 3 best of NH awards. A Grand B & B amidst the White Mountains. B & B offering Fireplaces, decks, Jacuzzis, outdoor pool, golf, ski & snowshoe on site. Casual tavern and award winning restaurant, wine list. Spectacular views wherever you look!
|Page: 12 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |
Find the perfect accommodations for vacations, romantic weekends and adventurous getaways. Find the right accommodations for your travels, from budget to luxury!
|Page: 12 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 |


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Originally written on Who Dat Dish | Last updated 12/5/11
IRVING, TX - DECEMBER 12: Wide receiver Joe Horn #87 of the New Orleans Saints runs between Nathan Jones #32 and Dexter Coakley #52 of the Dallas Cowboys on December 12, 2004 at Texas Stadium in Irving, Texas. (Photo by Ronald Martinez/Getty Images)
NFL and Drugs (Source: www2.tbo.com) This summer fierce legal battles erupted around the NFL – the players sued the league, the league sued the players, and even ex-players sued the league and the players — all over millions, and in some circumstances billions, of dollars in revenue. … [visit site to read more]Ex-New Orleans Saint Joe Horn among several players suing NFL over Toradol - Who Dat Dish - Who Dat Dish - A New Orleans Saints Fan Site - News, Blogs, Opinion and more.
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Summer GiftsMake their day extra special and send one of our beautifully presented summer gifts. This season, we've chosen some attractive options for you including crisp, sparkling wines, a selection of sumptuous hampers and some pretty planters to give colour and texture to their outdoor space.
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Tortilla Soup
From south of the border comes this spicy and hearty soup ladled over crisp tortilla strips and grated cheddar. The soup is chunky with chicken and avocado, but it's the tortillas that give it a slight thickness and a special flavor.
Level: Moderate
Serves: 4
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- 6 tbsp. Cooking oil
- Eight corn tortillas
- 1 onion
- 4 large cloves garlic
- 1 tbsp. paprika
- 2 tsp. ground cumin
- 1 tsp. ground coriander
- 1 tsp. chili powder
- 0.25 tsp. cayenne
- 1.50 qt. canned low-sodium chicken broth or homemade stock
- 3 c. canned crushed tomatoes in thick puree (one 28-ounce can)
- 2 bay leaves
- 2.50 tsp. salt
- 0.25 c. lightly-packed cilantro leaves
- 1.75 lb. boneless
- 1 avocado
- 0.25 lb. Cheddar
- Lime wedges
- In a large heavy pot, heat the oil over moderately high heat. Add half the tortilla strips and cook, stirring, until pale golden, about 1 minute. Remove with a slotted spoon and drain on paper towels. Repeat with the remaining tortilla strips.
- Reduce the heat to moderately low. Add the onion, garlic, and spices; cook, stirring, for 5 minutes. Add the broth, tomatoes, bay leaves, salt, cilantro leaves, if using, and one-third of the tortilla strips. Bring to a simmer. Cook, uncovered, for 30 minutes; remove the bay leaves.
- In a blender, puree the soup in batches; pour it back into the pot. Add the chicken, bring the soup back to a simmer, and cook until just done, about 1 minute. Stir in the avocado.
- To serve, put the remaining tortilla strips in bowls, top with the cheese, and pour in the soup. Sprinkle with the chopped cilantro, if using, and serve with the lime wedges.
- Wine Recommendation: A Rioja Reserva, with its complex combination of spicy, earthy, and fruity flavors, will echo the taste of the soup beautifully.
What do you think?
These Berry Bourbon Creamsicles Are Ready To Win Your July 4th
Bourbon and berries: A new American tradition.
Lidey's Table: This Addictive Chipotle Hummus Jump-Starts Any Party
Take Ina's advice and make this can't-resist appetizer in advance.
More From Meals & Cooking


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Mike and Tommy answer fans' questions in the TD Bank Mailbag.
Here's a question that was asked PRIOR to the C's win over the Jazz. We capitalize prior for a reason:
The Celtics held off the Jazz Wednesday night, 98-93, and it was what they did on the defensive end in the end of the game that really mattered.
Photos and video clips are buzzing through the Internet the day after Jeff Green threw down a monster dunk against the Utah Jazz Al Jefferson on Wednesday at the TD Garden.
BOSTON The Boston Celtics once again found themselves in a nail-biter that wasn't decided until the final seconds of play.
CSNNE's Jessica Moran checks in with the day's top headlines.
The Boston Celtics squeaked out a 98-93 win over the Utah Jazz for their third straight victory.


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Photos of Mischa at a couple events from the same night in New York have been added:
– Style 360 Presents Sachika Fashion Show – After Party
– Karen Elson For Nine West Vintage America Collection
LadyGunn magazine have posted a feature on "My Boyfriend was Stolen By Tron", the art piece by Mischa and Ericka Clevenger. Head on over to the LadyGun website to read more about it. Mischa will also be featured in the upcoming Fall issue of the magazine.
A photo of Mischa at the Carrera Sunglasses Escape held in West Hollywood has been added. I'm not sure if there are more photos from this event, but this is all I could find.
As part of Mischa's trip for the Shanghai Film Festival, she also attended a press conference. It took way longer than expected to get the photos, but finally they've been added.
There is also a YouTube where you can see Mischa's interview. She comes on after the singing man.
Photos of Mischa at the opening ceremony of the 14th Shanghai International Film Festival have been added.Hopefully more photos will be added later.
I posted the candids of Mischa heading to the Pia Michi event below, but here finally are pictures of Mischa actually at the event. The launch party was held at Iberica restaurant in London.
Following the event, Mischa headed to The Box nightclub. Seems like co-star Ashley Madekwe was also there.
Candids of Mischa leaving Mahiki nightclub in London have been uploaded. I think I uploaded a couple of double shots in this album by mistake.
A stunning/radiant Mischa stepped out to attend the No-Tell Motel Southern Comfort Room Launch at Mahiki London. Beautiful!


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Special Events
- $25 Under 35
- FREE Movie Night
- Gala
- John R. Hamilton Mock Trial
- Open House
- How to Submit
- History & Updates
"We love OST and are extremely thankful they are in Orlando and serving the community."
– Orlando Shakes Audience Member
Join us for a FREE Open House to celebrate our 27th Season! Festivities include crafts and coloring for the little ones, meet-and-greet photo opportunities with costumed characters, and more. This annual event is fun for the entire family!
Check back later for more details!


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Polypeptides corresponding to mature human serum albumin residues 1 to n, where n is between 369 and 419 inclusive, are useful as substitutes for albumin in the treatment of burns and shock in humans, the clearance of undesirable compounds, (such as bilirubin) from human blood, in laboratory growth media and in HSA assays. The polypeptides may be produced by recombinant DNA techniques, especially in yeast.
1. A method of expanding the plasma volume of the blood of a mammal, comprising administering intravenously to said mammal an effective amount of a polypeptide which is
an N-terminal fragment of mature human serum albumin (HSA) consisting of residues 1 through n, where n is from 369 to 419, or
an oncotically active variant of said fragment, wherein said variant is a polypeptide of at least 360 and not more than 430 amino acids, exhibits at least 80% sequence identity with the corresponding HSA N-terminal fragment, and which differs from said fragment only by conservative substitutions.
2. A method according to claim 1, wherein the polypeptide is selected from the group consisting of HSA(1-373), HSA(1-388), HSA(1-389), HSA(1-390), HSA(1-407), and a variant of one of the foregoing HSA fragments, said variant exhibiting at least 80% sequence identity with the corresponding HSA N-terminal fragment and differing therefrom only by conservative substitutions.
3. A method according to claim 1 wherein the administered polypeptide is HSA(1-387).
This application is a continuation of application Ser. No. 07/687,211, filed Apr. 18, 1991, now abandoned, which is a divisional of Ser. No. 07/263,211, filed Oct. 27, 1988, now abandoned.
This invention relates to a novel polypeptide molecule which can be produced by recombinant DNA technology and can be used for many of the existing applications of human serum albumin.
Human serum albumin (HSA) is the most abundant plasma protein, contributing 60% w/w of the total protein content of the plasma. A molecule of HSA consists of a single non-glycosylated polypeptide chain of 585 amino acids of formula molecular weight 66,500. The amino acid sequence of HSA has been established by protein sequence analysis (Meloun et al, 1975, "Complete amino acid sequence of human serum albumin" FEBS. Letters 58:1, 136-317; Behrens et al, 1975, "Structure of human serum albumin" Fed. Proc. 34, 591) and more recently by genetic analysis (Lawn et al, 1981, Nucleic Acids Research 9, 6102-6114). Although there have been discrepancies between the amino acid sequences as published (some being attributable to polymorphisms), FIG. 1 represents the amino acid sequence currently believed to be most representative of the HSA present within the human population.
Because of its relatively small molecular weight and net negative charge at physiological pH (Peters, 1970, "Serum albumin", Adv. Clin. Chem. 13, 37-111), HSA contributes 85% of the osmotic effect of normal plasma. Thus HSA is the principal regulator of plasma volume. A secondary role of HSA is to bind small molecules produced by catabolic processes (for example fatty acids and bilirubin). Albumin represents the principal means for the transport of these key metabolites, which are poorly soluble at physiological pH. Physical, chemical, immunological and limited proteolytic studies of HSA have shown that the molecule is composed of regions of polypeptide chains which retain their conformation after separation from the parent molecule by enzymatic means. These polypeptide chains retain their binding capabilities thereby facilitating the mapping of binding sites for bilirubin, fatty acids and other small molecules to particular regions of the polypeptide chain (Kragh-Hansen, 1981, "Molecular aspects of ligand binding to serum albumin", A. Soc. Pharm. Expt. Ther. 33, 1, 17-53). Much of the information in this area has been reviewed (Brown and Shockley, 1982, "Serum albumin: structure and characterisation of its ligand binding sites").
The indications for the clinical use of therapeutic concentrates of HSA are related principally to its oncotic action as a plasma volume expander. Concentrates of HSA have been used therapeutically since the 1940's, in particular in cases of shock, burns, adult respiratory distress syndrome, and cardiopulmonary bypass. Albumin has also been used in cases of acute liver failure, following removal of ascitic fluid from patients with cirrhosis, after surgery, in acute nephrosis, in renal dialysis, and as a transport protein for removing toxic substances, such as in severe jaundice in haemolytic disease of the new born.
In addition to its use as a therapeutic agent, HSA is a major component of serum added to media used to support the growth of mammalian cells in tissue culture. The consumption of serum and hence of albumin has been greatly increased over recent years as biotechnology and pharmaceutical companies have expanded their tissue culture for research and for production. There is a universal need for lower cost and better regulation of sera for these purposes.
It is known to manipulate the HSA-encoding DNA sequence to express a recombinant polypeptide in microorganisms. Indeed such a recombinant HSA polypeptide has been produced in bacterial species such as Escherichia coli (G.B. Patent No. 2 147 903B) and Bacillus subtilis (European Patent Application No. 86304656.1) and the yeast Saccharomyces cerevisiae (European Patent Publication No. 201 239, Delta Biotechnology Ltd.); thus it is generally accepted that a recombinant polypeptide essentially identical to natural HSA can be produced in a variety of microbial hosts by employing known methods. However, in all cases where recombinant HSA has been produced, the objective has been to produce a molecule which is "nature-identical" to HSA in structure and biological function.
It has now been found that it is advantageous to produce shorter forms of HSA.
One aspect of the present invention provides a polypeptide comprising the N-terminal portion of human serum albumin up to amino acid residue n, where n is 369 to 419, and variants thereof.
The novel polypeptides of the invention are hereinafter referred to as "HSA(1-n)".
The term "human serum albumin" is intended to include (but not necessarily to be restricted to) known or yet-to-be-discovered polymorphic forms of HSA. For example, albumin Naskapi has Lys-372 in place of Glu-372 and pro-albumin Christchurch has an altered pro-sequence. The term "variants" is intended to include (but not necessarily to be restricted to) minor artificial variations in residues 1 to n (such as molecules lacking one or a few residues, having conservative substitutions or minor insertions of residues, or having minor variations of amino acid structure). Thus polypeptides which have 80%, preferably 85%, 90%, 95% or 99%, homology with any HSA (1-n) compound are deemed to be "variants". Such variants are preferably 360 to 430 amino acids long, more preferably 369 to 419 amino acids long. It is also preferred for such variants to be physiologically equivalent to HSA (1-n) compounds; that is to say, variants preferably share at least one pharmacological utility with HSA (1-n) compounds. Furthermore, any putative variant which is to be used pharmacologically should be non-immunogenic in the animal (especially human) being treated.
Conservative substitutions are those where one or more amino acids are substituted others having similar properties such that one skilled in the art of polypeptide chemistry would expect at least the secondary structure, and preferably the tertiary structure, of the polypeptide to be substantially unchanged. For example, typical such substitutions include alanine or valine for glycine, arginine or asparagine for glutamine, serine for threonine and histidine for lysine. Variants may alternatively, or as well, lack up to ten (preferably only one or two) amino acid residues in comparison with any given HSA (1-n); preferably any such omissions occur in the 100 to 369 portion of the molecule (relative to mature HSA itself). Similarly, up to ten, but preferably only one or two, amino acids may be added, again in the 100 to 369 portion for preference. The term "physiologically functional equivalents" also encompasses larger molecules comprising the said 1 to n sequence plus a further sequence at the N-terminal (for example, pro-HSA(1-n), pre-pro-HSA(1-n), met-HSA(1-n), and HSA(1-n) having a suitable leader sequence which is not necessarily native to HSA.
If the HSA (1-n) is to be prepared by culturing a transformed yeast (S. cerevisiae) as is described in more detail below, the leader sequence may, for example, be that found naturally with the yeast alpha-factor protein. C-terminal fusion products with other polypeptides of interest may be produced. Known forms and fragments of HSA are clearly to be regarded as excluded from the above definition, for example HSA(1-187), which was a peptic fragment produced in low yield (Geisow and Beaven, Biochem. J. 161, 619-624, 1977 and ibid. 163, 477-484, 1977. These prior articles identify the fragment as 1-386, but it has since become apparent (see, for example, Lawn et al, op-cit.) that this is due to the authors' use of incorrect published sequence information and that the fragment was in fact 1-387). Similarly, a C-terminal fusion protein comprising HSA (1-n) and the remaining HSA residues (numbers n+1 to 585) is not claimed as part of the invention.
Particularly preferred novel HSA(1-n) compounds include HSA(1-373)(i.e. C-terminal Val), HSA(1-388) (i.e. C-terminal Ile), HSA(1-389)(i.e. C-terminal Lys), HSA(1-390)(i.e. C-terminal Gln) and HSA(1-407)(i.e. C-terminal Leu).
The HSA(1-n) molecules are preferably produced by means of recombinant DNA technology (optionally followed by proteolytic digestion), rather than by chemical or enzymatic degradation of natural HSA, or by peptide synthesis. In the case of enzymatic degradation, for example, a trypsin-like enzyme will cleave HSA between Lys(389) and Gln(390) but also concomitantly at other cleavage sites. In the future, peptide synthesis may become more feasible for molecules as long as 419 amino acids, but at present is not a practical proposition. Expression in yeast is particularly preferred.
It has been found that, at least in some situations where the HSA(1-n) compound is produced by culturing a transformed host, some HSA(1-n) compounds which are longer than HSA(1-387) are proteolytically digested back to HSA (1-387) by the enzymes which are naturally present in the system. Thus, one can, if desired, use a nucleotide sequence corresponding to a given HSA(1-n) compound in order to prepare another HSA(1-n) compound.
The new molecules herein described can be used as an effective substitute for either natural HSA or nature-identical recombinant HSA as a plasma volume expander. An advantage of HSA(1-n) over natural HSA and recombinant nature-identical HSA relates to the efficacy of raising the colloid osmotic pressure of blood. The smaller molecular weight (approximately 44 kilo-daltons) of the protein of the present invention means that an individual protein dose of only one-half to two-thirds that of natural-HSA or natural-identical recombinant HSA will be required for the equivalent colloid osmotic effect. Consequently, any process for the production of this novel polypeptide by means of recombinant DNA technology may afford significant economic advantages over known processes for the production of nature-identical recombinant HSA, since substantially less proteinaceous material is required to be produced for an effective dose.
Thus, a second aspect of the invention provides a pharmaceutical composition comprising HSA(1-n)plus, where HSA(1-n)plus is HSA(1-n) as defined above or any HSA(1-n) molecules which are known per se but have not been proposed for pharmaceutical use.
HSA (1-387) which, as discussed above, was a fragment produced by chance in a prior art peptic digest of HSA, is particularly preferred as the HSA(1-n)plus in such a pharmaceutical composition. The composition may comprise "variants" of HSA (1-387) as defined above.
A third aspect provides a method of treating a human for shock, burns or other conditions in which albumin is indicated, comprising administering intravenously a blood-bulking or blood-clearing effective non-toxic amount of a sterile non-pyrogenic solution of a polypeptide comprising HSA(1-n)plus.
Further aspects of the invention include (a) vectors, plasmids and transformed microorganisms, including cell lines, encoding HSA(1-n)plus expression; (b) processes for the production of HSA(1-n)plus comprising the fermentation under suitable conditions of a microorganism (including a cell line) so transformed as to express HSA(1-n)plus; and (c) laboratory media comprising HSA(1-n)plus.
A further advantage of at least some HSA(1-n) plus molecules over nature-identical recombinant HSA is that their smaller size and thus reduced amino acid content has been found to lead to an increase in the yield obtained (molecules per cell dry weight) in microbial hosts relative to that obtained currently for nature-identical recombinant HSA. Thus, not only has it been found that the scale of the process can be reduced, but also productivity in the recombinant host organism can be enhanced.
The compounds of the invention may be used as blood-bulking (plasma-expanding) agents in analogous ways and in analogous formulations as HSA itself except that the dose of the HSA(1-n)plus compound (in terms of weight) will generally be less than that of HSA as the oncotic effect of the former is greater. The pharmacist or clinician skilled in the art will readily be able to determine by routine and non-inventive experimentation the optimum dose of the HSA(1-n)plus compound. Generally, the amount of HSA(1-n)plus which is administered will be about two-thirds of the amount of HSA which would be administered.
HSA (1-n) plus compounds may also be used as: (1) substitutes for HSA or, more commonly, bovine serum albumin (BSA) in tissue culture media, thereby reducing the risk of contamination of the medium with, for example, viruses and mycoplasmas; (2) substitutes for BSA in the stationary phase in liquid chromatography for resolution of enantiomers and so on.
The invention will now be illustrated by way of example and with reference to the drawings, in which:
FIG. 1 depicts the amino acid sequence currently thought to be the most representative of natural HSA, with (boxed) the alternative C-termini of HSA(1-n);
FIG. 2 depicts the DNA sequence coding for mature HSA;
FIG. 3 illustrates, diagrammatically, the construction of mHOB16;
FIG. 4 illustrates, diagrammatically, the construction of pHOB31; and
FIG. 5 is a copy of a rocket electrophoretogram showing the increased yield of HSA(1-389) over complete HSA.
FIG. 6 illustrates the construction of pDBD3 from pDBD2 and pMA91.
FIG. 7 likewise depicts the construction of pDBD4 from pDBD2 and pMA91.
FIG. 8 compares the binding of bilirubin to HSA (1-387) (delta 21) and to clinical grade HSA.
FIG. 9 shows that HSA (1-387)(delta 21) yields a colloid osmotic pressure about one-third higher than that of full-length HSA at a given protein concentration.
Standard recombinant DNA procedures are as described by Maniatis et al (1982) unless otherwise stated. Construction and analysis of M13 recombinant clones was as described by Messing (1983) and Sanger et al. (1977).
The human serum albumin coding sequence used in the construction of the following molecules is derived from the plasmid M13mp19.7 (European Patent Application No. 201 239, Delta Biotechnology Ltd.) or by synthesis of oligonucleotides equivalent to parts of this sequence. Oligonucleotides were synthesised using phophoramidite chemistry on a Applied Biosystems 380B oligonucleotide synthesizer according to the manufacturer's recommendations (AB Inc., Warrington, Cheshire, England).
An expression vector was constructed in which DNA encoding the HSA secretion signal and mature HSA up to and including the 389th amino acid, lysine, was placed downstream of the S. cerevisiae phosphoglycerate kinase gene (PGK) promoter and followed by a stop codon and the PGK terminator of transcription. This vector was then introduced into S. cerevisiae by transformation and directed the expression and secretion from the cells of a molecule representing the N-terminal 389 amino acids of HSA.
An oligonucleotide was synthesised (Linker 1) which represented a part of the known HSA coding sequence (FIG. 2) from the PstI site (1092, FIG. 2) to the codon for valine 381 wherein that codon was changed from GTG to GTC: ##STR1## Linker 1 was ligated into the vector M13mp19 (Norrander et al, 1983) which had been digested with PstI and HincII and the ligation mixture was used to transfect E. coli strain XL1-Blue (Stratagene Cloning Systems, San Diego, Calif.). Recombinant clones were identified by their failure to evolve a blue color on medium containing the chromogenic indicator X-gal (5-bromo-4-chloro-3-indolyl-β-D-galactoside) in the presence of IPTG (isopropylthio-β-galactoside). DNA sequence analysis of template DNA prepared from bacteriophage particles of recombinant clones identified a molecule with the required DNA sequence, designated mHOB12 (FIG. 3).
M13mp19.7 consists of the coding region of mature HSA in M13mp19 (Norrander et al, 1983) such that the codon for the first amino acid of HSA, GAT, overlaps a unique XhoI site thus: ##STR2## (EPA No. 210239 A1). M13mp19.7 was digested with XhoI, made flush-ended by S1-nuclease treatment and was then ligated with the following oligonucleotide (Linker 2): ##STR3##
The ligation mix was then used to transfect E. coli XL1-Blue and template DNA was prepared from several plaques and then analysed by DNA sequencing to identify a clone, pDBD1 (FIG. 4), with the correct sequence.
A 1.1 kb HindIII to Pst1 fragment representing the 5' end of the HSA coding region and one half of the inserted oligonucleotide linker was isolated from pDBD1 by agarose gel electrophoresis. This fragment was then ligated with double stranded mHOB12 previously digested with HindIII and PstI and the ligation mix was then used to transfect E. coli XL1-Blue. Single stranded template DNA was prepared from mature bacteriophage particles of several plaques. The DNA was made double stranded in vitro by extension from annealed sequencing primer with the Klenow fragment of DNA polymerase I in the presence of deoxynucleoside triphosphates. Restriction enzyme analysis of this DNA permitted the identification of a clone with the correct configuration, mHOB15 (FIG. 4).
The following oligonucleotide (Linker 3) represents from the codon for the 382nd amino acid of mature HSA (glutamate, GAA) to the codon for lysine 389 which is followed by a stop codon (TAA) and a HindIII site and then a BamHI cohesive end: ##STR4##
This was ligated into double stranded mHOB15, previously digested with HincII and BamHI. After ligation, the DNA was digested with HincII to destroy all non-recombinant molecules and then used to transfect E. coli XL1-Blue. Single stranded DNA was prepared from bacteriophage particles of a number of clones and subjected to DNA sequence analysis. One clone having the correct DNA sequence was designated mHOB16 (FIG. 4).
A molecule in which the mature HSA coding region was fused to the HSA secretion signal was created by insertion of Linker 4: ##STR5## into BamHI and XhoI digested M13mp19.7 to form pDBD2 (FIG. 5). In this linker the codon for the fourth amino acid after the initial methionine, ACC for threonine in the HSA pre-pro leader sequence (Lawn et al, 1981), has been changed to AGC for serine to create a HindIII site.
The 5' end of this construction was removed as a BamHI to PvuII fragment and ligated with the PvuII to BamHI fragment of double stranded mHOB16 (representing the 3' end of the truncated HSA gene) into pMA91 (Mellor et al, 1983) at the BglII site to form pHOB31 (FIG. 4). This molecule contains the truncated HSA coding region with the HSA secretion signal between the S. cerevisiae PGK gene promoter and terminator such that the 5' end of the gene abuts the promoter. The molecule also contains a selectable marker for yeast transformation, LEU2, and part of the yeast 2um plasmid to permit autonomous replication in yeast.
The plasmid pHOB31 was introduced into S. cerevisiae AH22 (Hinnen et al, 1978) by transformation using standard procedures (Beggs, 1978). Purified transformants were grown in YEPD broth (1% yeast extract, 2% peptone, 2% glucose) for 3 days at 30� C. and the culture supernatant was then analysed, successfully, for the presence of HSA-related material by rocket gel electrophoresis. FIG. 5 shows the electrophoretogram: the yield of HSA-related material from transformants harboring a plasmid encoding HSA(1-389) is demonstrably higher than the yield from a transformant secreting mature, natural, HSA.
However, production of HSA (1-389) gave a product indistinguishable from HSA (1-387)(see Example 2) by both amino-terminal and carboxy-terminal sequence analysis. This is probably explained by the efficient removal of the COOH-terminal sequence Ile-Lys.
The construction of a plasmid encoding HSA (1-387) was identical to the procedure for construction of the HSA (1-389) plasmid, pHOB31, except that the linker 3 was substituted by linker 5 (shown below) which represents the region from the codon for the 382nd amino acid of mature HSA (glutamate, GAA) to the codon for leucine 387 which is followed by a stop codon and a HindIII site and then a BamHI cohesive end: ##STR6## The remainder of the construction was as detailed above for pHOB31 and resulted in the plasmid pDBD5.
In order to construct a plasmid encoding HSA (1-369), a linker was synthesised representing the region from the PstI site of mature HSA (position 1092, FIG. 3) to the codon for cystine 369 which was followed by a stop codon (TAA), a HindIII site and then a BamHI cohesive end: ##STR7##
This linker was ligated with the BamHI PstI fragment of pDBD2, representing the 5' part of preproHSA, into pMA91 at the BglII site. A plasmid with the correct configuration was termed pDBD3 (FIG. 6).
Production of HSA (1-369) by culturing S. cerevisiae transformed with pDBD3 gave low yields, indicating that the product may have been unstable in the yeast expression system used.
For the construction of a plasmid encoding HSA (1-419) the BamHI-HincII fragment of pDBD2 was ligated with an annealed self-complementary oligonucleotide (linker 7):
and then the ligation mix was digested with BamHI and the fragment was ligated into pMA91 to give pDBD4 (FIG. 7). In this construct the HincII site (1256, FIG. 3) of pDBD2 creates a blunt end after the second base of the codon for serine 419 and this codon is reformed by the linker 6 such that this codon is followed by a stop codon, a HindIII site and a BamHI site.
Expression of HSA (1-419) via plasmid pDBD5 in S. cerevisiae produced a molecule with the correct amino terminal sequence (Asp-Ala-His . . . ) but leucine and not serine was the COOH-terminal residue. Attempts to isolate the COOH-terminal peptide using a covalent label which should attach to cysteine 392 also were unsuccessful. It was concluded that proteolysis of part of the COOH-terminus of HSA (1-419) occurred. This is consistent with the observation of a small percentage of proteolysis in the same position of full-length HSA produced in an analogous manner in yeast. (Sleep et al, 1988).
A laboratory fermenter is filled to half its normal working volume with an initial "batch" medium containing 50 ml/l of a salts mixture (containing 114 g/l KH2 PO4, 12 g/l MgSO4, 3.0 g/l CaCl2.6H2 O, 2.0 g/l Na2 EDTA; 10 ml/l of a trace elements solution containing 3 g/l ZnSO4.7H2 O, 10 g/l FeSO4.7H2 O, 3.2 g/l MnSO4.4H2 O, 79 mg/l CuSO4.5H2 O, 1.5 g/l H3 BO3, 0.2 g/l KI, 0.5 g/l Na2 MoO4.2H2 O, 0.56 g/l CoCl2.6H2 O, 75 ml/l H3 PO4 ; 20 g/l sucrose; 50 ml/l of a vitamins mixture containing 1.6 g/l Ca pantothenate, 1.2 g/l nicotinic acid, 12.8 g/l m inositol, 0.32 g/l thiamine HCl and 8 mg/l pyridoxine HCl and 8 mg/l biotin. An equal volume of "feed" medium containing 100 ml/l of the salts mixture, 20 ml/l of trace elements solution 500 g/l sucrose and 100 ml/l vitamin solution is held in a separate reservoir connected to the fermenter by a metering pump.
The fermenter is inoculated with Saccharomyces cerevisiae which has been transformed as above with plasmid pDBD3 from Example 2. The pH is maintained at 5.7�0.2 by automatic addition of ammonia or sulphuric acid, the temperature is kept at 30� C. and the stirred speed is adjusted to give a dissolved oxygen tension (DOT) of >20% air saturation at 1 v/v/min air flow rate. When the initial substrate has been consumed, the metering pump is turned on, maintaining a growth rate of approximately 0.15 h-1. The pump rate is increased to maintain this growth rate until the stirrer speed reached its maximum value at which point it is not possible to increase the pump rate any further without causing the DOT to fall below 15% air saturation which is the minimum value permitted to occur. PPG 2000 is added in response to a foam sensor. None is added until over 50% of the feed solution had been added. The final level of addition is 0.2 g/l.
HSA(1-387) is secreted into the medium.
Binding of the heme metabolite, bilirubin, to HSA (1-387) was carried out by a fluorescence enhancement method (Beaven and Gratzen (1973) Eur. J. Biochem. 33, 500-510). FIG. 8 shows that the enhancement of bilirubin fluorescence as a function of protein/bilirubin ratio is indistinguishable for HSA(1-387) and clinical grade HSA.
The interaction of HSA and bilirubin is very sensitive to the conformation of the protein (Beaven and Gratzen, loc. cit.) and these results indicate that no gross alteration in conformation of the region of HSA represented by HSA(1-387) has occurred through the expression of a shorter molecule.
HSA(1-387) was concentrated in 0.9% w/v saline to a final protein concentration of 54 mg/ml. Dilutions of this concentrate, together with dilutions of a clinical grade HSA (100 mg/ml), were compared for osmotic effect in a colloid osmometer. FIG. 9 indicates that HSA(1-387) gives a colloid osmotic pressure approximately one-third higher than that of full-length HSA at a given protein concentration. Importantly, the increase in colloid osmotic pressure with protein concentration is approximately linear over a range up to 5% w/v, which represents the concentration in plasma.
This indicates that HSA(1-387) does not self-associate appreciably within a useful working clinical concentration range.
The HSA(1-n)plus of the invention may be presented in container sizes ranging from 20 ml to 500 ml, with the concentration thereof varying (typically) from 2% to 17% for example 3%, 13% or 17%.
The solution for administration is sterile and pyrogen free. A 3% solution is osmotically similar to human plasma. At least 96% of the total protein is preferably albumin. The sodium ion content is generally between 130-160 mmol/liter and the potassium ion content is generally not more than 2 mmol/liter. The pH is adjusted to 6.9�0.5. The concentration of citrate is generally no more than 20 mmol/liter and may be absent altogether.
Stabilizers may be used, for example either 0.16 millimole sodium acetyl tryptophanate, or 0.08 millimole sodium acetyl tryptophanate and 0.08 millimole sodium caprylate per gram of HSA(1-n)plus.
Beggs, J. D. (1978), Nature, 275, 104-109.
Brown, J. R. and Shockley, P., (1982) in "Lipid-Protein Interactions" 1, 25-68, Eds. Hayes, O. and Jost, P. C.
Hinnen, A. et al (1978), Proc. Natl. Acad. Sci. U.S.A., 75, 1929-1933.
Lawn, R. M. et al (1981), Nucl. Acid. Res. 9, 6103-6114.
Maniatis, T. et al (1982), Molecular cloning: A laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, New York.
Mellor, J. et al (1983), Gene, 24, 1-14.
Messing, J. et al (1983), Methods Enzymol. 101, 20-78.
Norrander, J. et al (1983), Gene, 26, 101-106.
Sanger, F. et al (1977), Proc. Natl. Acad. Sci. U.S.A., 74, 5463-5467.
Sleep, D. Belfield, G. P. and Goodey, A. R. (1988) Yeast 4, S168.
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When speaking out about human rights might land you in jail
July 02, 2015 , 3:53 PM by Jo Printz | Comments (0)
More than 40 current and former workers at Australia's detention centres on Nauru and Manus Island published an open letter on July 1, challenging the government to prosecute them under new secrecy laws for speaking out over human rights abuses.
Here's part of that letter... "Today the Border Force Act comes into force. It includes provision for a two-year jail sentence for 'entrusted persons' such as ourselves if we continue to speak out about the deplorable state of human rights in immigration detention without the express permission of the Minister for immigration and border protection.
This strengthens the wall of secrecy which prevents proper public scrutiny. We have advocated, and will continue to advocate, for the health of those for whom we have a duty of care, despite the threats of imprisonment, because standing by and watching sub-standard and harmful care, child abuse and gross violations of human rights is not ethically justifiable."
Earlier this year, Mornings spoke with Central Victorian mental health nurse Chris Cummins, who had just returned to Australia after five years working with asylum seekers on Christmas Island - as team leader of the Torture and Trauma Counselling Service - and we wanted to find out what she thought of her colleagues letter and the new legislation.
Hear her response below:
Read more


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VACATION BIBLE SCHOOL: Updated for July 4
'Inside Out' at HR Church of Christ
Death notice for July 4
Wayne Jones
Yesteryears: Swimming pool bond measure passes 398 to 61 in 1945
With a majority of better than 6 to 1,000 voters of Hood River City endorsed the $60,000 bond issue, proceeds of which are destined to be used for the construction of a swimming pool. The vote was: Yes, 398; No, 61. While the location of the pool has not been definitely decided upon, it is anticipated that a site near the city high school will be selected, and it may be incorporated into a wider plan for a recreation park at the same location.
BBBS friends learn windsurfing basics
On a pleasantly warm and breezy day, Big Brothers Big Sisters of the Columbia Gorge held its annual Windsurfing Clinic at The Hook.
MID VALLEY NEWS: Celebrate the Fourth in Odell
First, a correction: I inadvertently typed the wrong date for the 50-Plus Expo coming to the Hood River County Fair on Senior's Day in my last column — it will be on July 23, to correlate with the special admission price to the fairgrounds for seniors on that day.
Castillo-Flores named to White House internship
Yonny Castillo-Flores, a 2011 Hood River Valley High School graduate, has been named a White House Intern for summer 2015.
44 Trails Association hosts Trailgate event on July 11
Celebrate the world of Mt. Hood single track at Trailgate, hosted by the 44 Trails Association and the Mt. Hood National Forest.
Bagge Gardens holds last gospel garden concert
Bagge Gardens, located at 1805 Carroll Rd., Mosier, will hold its last-ever gospel music garden concert on July 18 beginning at 5:30 p.m.
Prevention Dept. partners listed
Here is the full list of recipients of certificates of appreciation from the Hood River County Prevention Program last month:
Katie Crafts is addicted to high latitudes: Wanders and wonders in Antarctica and Alaska
"How much money do you have?"
Kiters and barge in a river drama
Sam Grotte of White Salmon enjoys taking scenic photos and his close-ups of hummingbirds are true micro-examinations of the world around us.
Cyclist rolls through Hood River on cancer-fighting 'Victory Tour'
A New Orleans man pedaled through the Gorge last Friday — part of a 7,200-mile cross-country bike ride across the United States honoring his wife's battle with pancreatic cancer.
The Ale List: Solog Fest returns
The second annual Solog Festival will return to Parkdale on an August date to be announced, said brewer Jason Kahler of one of two breweries involved in the collabora-fest, Solera Brewery of Parkdale and Logsdon Farmhouse Ales of Hood River. Watch The Ale List for details.
HR Parks District presents Guignard Road park proposal
A new park for the south part of Hood River took one step closer to reality last week.
Port announces waterfront closures for July 4 festivities
The Fourth of July holiday will limit access to several Port of Hood River waterfront properties beginning Friday, July 3 when the Hood River Eyeopeners Lions Club begins setting up its fireworks display just west of the mouth of the Hood River.
Pet of the Week: Joonie is 'ready for cuddles'
This week volunteers at Adopt A Dog have selected Joonie as the program's adoptable pet of the week:
Sheriff Log, June 22 to 28
All calls were responded to within the County of Hood River.
Passions run high at Nestlé town hall
More than 120 people filled the Cascade Locks City Hall gym Thursday to discuss Nestlé's proposed bottling plant. Tension mounted as those in favor and opposed raised their voices with comments and questions.
Letters to the Editor for July 1
Reality; Yes to Nestlé;Make a change
Extreme Need: No ban on fireworks — just be careful
Do you think Sunday's thunder storm was loud?


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NewsBusted 4/02/13
02 Apr, 2013 by Newsbusted
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Father Hands Out Haymakers to Teenage Girl Punks for Attacking His Daughter [Video]Jun 29, 2015 | By Terresa Monroe-Hamilton
Facebook36.9kTwitter35Email1 This dad punches the crap out of two girls attacking his daughter. Tell me you wouldn't do the same if a pack of thugs were beating on your child....Read More
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Narrow your search
The brains behind Moore's Law, which says processing power should increase exponentially every couple years, says his 1965 prediction was initially made looking only 10 years out.
An international tech competition targets Silicon Valley's diversity problem, aiming to inspire girls around the world into taking up -- and sticking with -- technology.
Samsung plans to launch "several" Tizen smartphones by the end of this year across a wide range of prices, according to a new report.
Everything you need to know about today's Apple announcements.
Maker of fitness- and health-tracking gadgets raises $731.5 million, for a valuation of about $4.1 billion.
Commentary: Apple's chief explains why having more women and minorities in tech matters to Apple and the rest of the industry. In short, diversity is the key to future success.
Mozilla is working on a project that could let a few key Android apps like WhatsApp run on its fledgling mobile operating system, which could keep its prospects alive.
This startup wants to put a desktop computer in the homes of two billion people who can't afford normal PCs. Here's how they are going to do it.
The chip designer is reportedly in talks to spend about $80 million on Israel-based Sansa Security, which would help it compete in the growing market for connected devices.
Google's experimental hardware division talks smart textiles, and controlling them just by the touch of your hand.


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With George Springer making his highly-anticipated MLB debut on Wednesday, we take a look at some of Houston's notable first-round draft picks and how they fared in their MLB debut with the Astros.
How big a blow is George Springer's injury for the Astros?
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Town Square
Review: 'Kung Fu Panda 2'
Original post made on May 27, 2011
Read the full story here Web Link posted Thursday, May 26, 2011, 3:01 PM
Comments (1)
a resident of another community
on May 27, 2011 at 1:30 pm
I'm taking my 5 year old daughter tomorrow. Can't wait. Here's a funny review from a Finnish film reviewer attempting to write in English. I think he likes it.
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Calave wine bar opens in Palo Alto
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Family Outing - Inside Out
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For those looking for better performance than the stock settings, use 384/1200 interactive instead of ondemand with a high max freq (I tried 1420 and 1536 steps). I did some tests and the first setup always yields better real life performance, better benchmarks, and better screen on time.
Wheatley on the other hand I have not tested yet.
Sent from my Galaxy Nexus using Tapatalk 2
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Black & Decker The Complete Guide to a Clutter-Free Home: Organized Storage Solutions & Projects
Creative Publishing | Philip Schmidt | 2009 | HQ PDF+OCR | ISBN: 1589234782 | 240 pages | 104 Mb
Finding adequate storage space is an ongoing battle in every household. The Complete Guide to a Clutter-Free Home provides inspired answers and dozens of step-by-step projects that offer creative solutions to common storage challenges. From understairs shelving to full-height medicine cabinets to laundry rooms and lumber racks, this book shows you how to find hidden storage space around your home and how to put it to use efficiently.
[Fast Download] Black & Decker The Complete Guide to a Clutter-Free Home: Organized Storage Solutions & Projects
60 Quick Luxury Knits: Easy, Elegant Projects for Every Day in the Venezia Collection from Cascade Y
How To Grow Organic Plants Indoors With LED Lights
Crochet Ever After: 18 Crochet Projects Inspired by Classic Fairy Tales
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Life Hacks: Any Procedure or Action That Solves a Problem, Simplifies a Task, Reduces Frustration, E
Simple Steps to Success: Orchids
Antique To Heirloom Jelly Roll Quilts: 12 Modern Quilt Patterns from Vintage Patchwork Quilt Designs
Illustrated Dictionary of Photography: The Professional's Guide to Terms and Techniques
Planet Earth: 25 Environmental Projects You Can Build Yourself
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Tablet peeped, even though Sony Ericsson claims it has no slate plans.
Sony Ericsson is working on a 4G tablet, with a prototype of the next-gen network-packing device snapped last week during Mobile World Congress in Barcelona.
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The slate, spied by eagle-eyed Dutch bloggers at the TDIA stand, appear to cut a chunky figure, with a bezel that would make even the current-generation iPad blush. According to reps on the stand, the Sony Ericsson 4G tablet will come loaded with Android, with a 3 megapixel camera up front for video calling.
Sony Ericsson last week denied it was working on a tablet, a claim seemingly back up by leaked designs and details of the Sony-only S1.
What do you make of the Sony Ericsson 4G tablet? Enough to make you hold fire before slapping down your hard-earned on one of the myriad new Android tabs? Tell us now on our Facebook and Twitter pages.
The 4G tablet might not be official but the Sony Ericsson Xperia Play has made its debut. Check out our hands-on video below.
Sony Ericsson Xperia Play video
Video Source: T3 Tech Videos
News via TechRadar and NieuweMobiel


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The Tal Law deals ONLY with yeshiva students. It has nothing to do with Israeli Arabs, who are not - and never have been - exempt from callup for military service. Ehud Barak can call Israeli Arabs up tomorrow, and if he did then they MUST report for service. No change in legislation is required: Barak merely needs to change the current administrative policy, which is to not bother to call them up.
If selected for publication, it will appear as soon as possible on Haaretz.com.


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"As a young ski racer, I couldn't find any women I could relate to as role models in the sport," Lynsey Dyer writes in the our January issue (which hits newsstands on January 7). So she sought out women who followed their passion fearlessly, whatever that passion was. Now Lynsey is one of those role models, and she's bringing together others like her to produce "Pretty Faces," an all-female ski and adventure-sports film.
Kickstarting "Pretty Faces"
Video: One-Legged Shred
"I want to show people that it's possible to be good at skiing if you have a disability," says Vasu Sojitra ("That Guy," BCM 93). Well, good seems like an understatement, and disability seems like a relative term. Vasu crushes it, as shown in this early-season edit. He and his friends at T-Bar Films produced it […]
The Art of the Up: Tips for Skinning Ease and Efficiency
Look at any beginner skinner, and you'll likely see an act of distress akin to uphill roller-skating on black ice. Look at someone who's been at it for a while, and they might appear as comfortable sidehilling a sheen of breakable crust as a child frolicking through a meadow. Here's how to become that skinner.
Ruthless Ambitions: Scouting steeps in Alaska's Ruth Amphitheater
Like a trip to Chamonix, one to Alaska's Ruth Amphitheater is a prerequisite to earning your "Serious Alpinist" merit badge. Andrew McLean had never been to either, and was painfully aware of his lowly Webelo (We Be Loyal Scouts) status until last spring when he had a chance to redeem himself with a trip to the Ruth. The Powderwhore's captured the trip in this year's film, "Elevation."
Working for the Weekend: Meathead Films' New Series
Happy Winter: Andreas Fransson's Musings
Andreas Fransson may seem like the maddest man on skis, ticking off unimaginably steep descents around the world. But he's also a yogi and deep thinker with endless musings on life in the mountains. Backcountry Managing Editor Tyler Cohen got to know Fransson last April on a trip to Chamonix, France, and he profiled the extreme […]
Pit Maneuvers: Snow pit tests and digging protocol
When was the last time you dug a snow pit? During that Avy 1 course you took a few years back? On that one hut trip last winter to a zone you'd never skied before? Digging pits is often seen as time consuming, but, in reality, there's no better way to understand the snowpack upon which you're traveling. Here are four videos to refresh your skills.
Bare-Ass Backcountry: Sweetgrass "Valhalla" Segment
Heaven on Earth: Holden Village, Wash.
Getting Caught in the Coast Range
Last April, pro skier KC Deane joined photographer Mason Mashon and friend Kye Peterson for a photo session near the Pemberton Icecap in British Columbia's Coast Mountains. KC and Kye skied a test run, made several ski cuts on their intended line and dropped a cornice nearby to no hazardous effect. But when KC dropped into his intended line, he took a wild ride.


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Live from the Biltmore - No Sinner kicks off their set with this doozie track.
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Date: 1908-09-08
Description: Photographic postcard of Signal Rock. Trees are in the foreground and on the the hill. Text below reads, "Signal Rock, Camp Douglas, Wis."
Date: 1910 ca.
Description: Postcard reads "New High School, Antigo, Wis."
Date: 1911
Description: Interior view of soda fountain and confectionary, part of Wilson's Drugstore.
Date: 1920 ca.
Description: Exterior of the La Crosse post office, which was destroyed in 1977.
Date: 1910 before
Description: A giant, oddly-shaped potato rests on a flatbed railroad car. A horse-drawn carriage with a driver is visible behind the train. White bags filled with pota...
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Description: A souvenir postcard from the 1935 National Model Airplane Championship that featured illustrations of racing planes powered by Shell aviation gasoline such...
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We are thrilled to feature a large selection of eeBoo! Founded in New York City by Mia Galison, eeBoo is an award-winning boutique toy manufacturer that creates a refreshing variety of toys, games, and gifts all decorated with unique artwork from well-loved children's book illustrators. Playtime favorites such as bingo games, memory games, card games, growth charts, and paper dolls by eeBoo are manufactured with the highest quality and encourage literacy, basic math, and story-telling skills.
Little Robot's Mission Write Me A Story
Little ones will get exposed to the delights of creative writing with our Little Robot's Mission Write Me A Story. This charming kit has everything your youngster needs to create an original story book they can hold onto forever. Each of the 50 pages in this book features 4 spaces at the top for stickers and lines below where your child can writ...More Details →
Circus Animals' Adventure Write Me A Story
Children can experience the joys of creative writing with our Circus Animals' Adventure Write Me A Story. This kit has everything your little author needs to create an imaginative story book they can hold onto forever. Each of the 50 pages in this book features 4 spaces at the top for stickers and lines below where your little one can write thei...More Details →
Mystery In The Forest Tell Me A Story
Melissa Sweet's whimsical forest creations come together to make the Mystery in the Forest: Tell Me a Story Creative Story Cards a true family favorite. Pre-schoolers will mix and match a total of 36 different cards in limitless combinations, creating original tales full of twists and turns that only a child's imagination could invent. The possi...More Details →
Little Robot Tell Me a Story Flashcard Storytelling Prompts
Send your little science-fiction enthusiast into the future with The Little Robot's Mission: Tell Me a Story Creative Story Cards. Saxton Freymann invented these robot rascals with pre-school imaginations in mind. Line up the cards to create a robot adventure tale in outer space. 36 separate cards encourage creativity and spark your child's imag...More Details →
Fairytale Mix-Up Tell Me A Story
Children will fall in love with Lizzy Rockwell's depiction of classic fairy tale characters and scenes in the Fairytale Mix-Ups Tell Me a Story Creative Card Set. All of your preschooler's favorite fairy tales are included. The possibilities are endless -- kids can combine a variety of fairytale worlds into one or more amazing stories as they pl...More Details →
Circus Animals Tell Me A Story Flashcard Storytelling Prompts
Your child's imagination will serve as the ringleader as Brandon Reese's circus-themed story cards prompt them to create their own wacky circus stories. 36 illustrated action cards featuring cats on stilts, juggling dogs, and a mischievous monkey will spawn creativity and originality. Play with your little one and help them along with ideas and ...More Details →


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'Revenge' recap: It's love or confusion as nemeses resurface in the Hamptons
"What's our motivation?"
Kara Clarke is back in everyone's lives — stopping by Grayson Manor to visit Victoria (read: see if she knows where Gordon is) and tell her how glad she is that she's safe (read: keep an eye on her). Victoria freaks out and calls Conrad; Kara could ruin the Graysons with information she might have! It's hard to believe people this scheme-y wouldn't have dealt with that possibility back when they got her out of town in the first place.
The unlikely changelings
It's just as hard to believe that nobody on "Revenge" who claims to have loved Young Amanda can tell that Present-Day Amanda isn't the same person — but the show's still going with that, as Kara is at the hospital and doting on newly de-coma'd Amanda and baby Carl. (Despite a fall that should probably have killed both of them, Carl is fine, Amanda has not a scratch on her and flawless hair even in the hospital, and they both get sent home about 36 hours after she wakes up.)
Only Mason Treadwell, who shows up at the hospital with a bouquet of flowers and a nose for the "Kara returns" story, has any idea — helping Amanda out of a wheelchair, Mason sees that a scar Amanda is supposed to have, from a fire in a foster home, is absent.
Mason should stir things up by exploiting that secret — but all the other plots in the episode just kind of lie there. The homeowner, Kenny, hooks Jack and Declan up with a good contractor, then offers to buy the bar and forgive Dec's debt, as predicted, but neither of the Porter brothers seems either thrilled or upset. Emily is sad in spite of herself that Kara doesn't recognize her — Nolan insightfully notes that that's "good for revenge-y stuff, but sad for you" — and she tells Amanda to tell Kara the truth, that she doesn't recognize her and can't forgive her. (She also tells Amanda that Kara tried to drown her when she was 5. When Amanda wonders how a mother could do that, we wondered if Kara is Emily's real mother. You never know on this show.) Kara gives a tearful speech about how well Amanda turned out, and Amanda, touched in spite of herself, forgives her, in defiance of orders — but then Emily's kind of like, "Well, it's just as well," and forgives Amanda for screwing it up, so you have to wonder whether Emily even has a plan at this point if it's this flexible. What does she want from Kara?
And then, after apologizing to Amanda for almost getting her killed, Emily admits that Carl is Jack's baby. Amanda forgives her, and is forgiven in turn for getting together with Jack in the first place when she knew Em had feelings for him, which is nice, we guess, but again — what is Emily's endgame here? We're not seeing a vengeance plan, just secrets for the sake of secrets.
David Clarke, still causing posthumous problems
Foxy Aiden is still operating as though Emily's actively engaged in destroying the Graysons. He's embedded at Grayson Global as Takeda's proxy, drinking martinis with Daniel and Ashley and nosing around. But Emily's keeping things from him, like the fact that she has Gordon's cell phone, and being as rude to him when he tries to be supportive as she usually is to Nolan: "I needed someone; you walked through the door." Foxy also tips Emily to the fact that Padma queried Grayson Global about David Clarke's initial investment in NolCorp; long story short, because David invested while he was at GG, GG has a controlling interest in NolCorp. Emily lets Nolan know that Padma blew up his spot, and he turns kind of green, but again, we can't visualize the endgame. Is Padma a Grayson plant? It doesn't make her any more interesting, or give her any chemistry with Nolan — and why is the show adding that intrigue when it can barely manage the ones it has?


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|Eli Manning and the Giants have the pieces to get to another Super Bowl. (AP)
EAST RUTHERFORD, N.J. -- The pass rusher hates the general manager, the coach might not yet trust the star wide receiver that shot himself in the foot and the competition is building what one player called a Dream Team. This, ladies and gentlemen, is the plight of the New York Giants.
The first day of training camp began with defensive lineman Osi Umenyiora promising an explosion to the media due to anger over his contract situation. The target was to be Jerry Reese, general manager of the team, whom Umenyiora claims broke a promise to re-do his deal. But the explosion never came because Umenyiora arrived late.
"Let's put it this way," said Umenyiora's agent, Tony Agnone, "Osi isn't a big fan of Jerry's. Osi is now waiting for one of two things. A new contract or a trade."
No one knows for sure which one will occur and there is indeed a third option: the Giants could do nothing and let Umenyiora stew. But one league source believes the Giants will eventually trade Umenyiora and Baltimore has emerged as a possible destination, though that seems unlikely. The Ravens jettisoned a number of talented veterans because of salary cap issues.
|More on Giants
|Related links
Coach Tom Coughlin explained the Umenyiora situation this way: "It's not what you'd like. You'd like everyone to be in the right frame of mind." That's one way of putting it. You'd also like the 300-pound defensive end not wanting to pummel the snot out of his general manager.
Then there's Plaxico Burress. Coughlin and Burress once went together like a bathtub and a plugged in hair dryer. Burress is out of prison and making the Plaxico NFL World Tour rounds with stops in New York and Pittsburgh. He met with Coughlin and the Giants coach said he enjoyed their conversation and interest was possible. Burress' destination is likely Pittsburgh or San Francisco.
So the Giants are dealing with Umenyiora, Burress, and their huge division rival, the Philadelphia Eagles, are building football's version of the Miami Heat. The Giants look dead, right?
Well ... maybe not.
Something about this Giants team is intriguing. Very intriguing. Under the radar, but intriguing.
Who will win the NFC East?
Total Votes: 20,602
I'm not the only one who believes this. When asking several coaches which NFC team could sneak out of its conference and give Green Bay a run they all said the Giants.
There's a feeling among some in football that if New York can overcome this dysfunctional beginning and maybe catch the Eagles a year or so away from its Super Bowl run, the Giants could do something. That's just a feeling, but it's more than an itch.
The reason is while Umenyiora may not like Reese, it is Reese who has built an incredibly fast, athletic roster that's deep enough to withstand the losses of players like Burress and Umenyiora. Burress is probably the best wide receiver the Giants ever had (yes better than Amani Toomer and Frank Gifford) and it says a lot about Reese that Burress can go to prison and Reese can find players to keep the offense churning at a respectable pace in his absence.
The defensive line is thick. The secondary just got help with first-round pick Prince Amukamara. There are solid backs and a good receiving corps. Quarterback Eli Manning has a Super Bowl ring and while Coughlin may be a hothead and stickler for players wearing the right colored socks, he's a talented coach.
The Eagles may run away with the division, but they may also find what the Heat discovered that expectations can be asthmatic and crushing. If the Eagles fall it could be the Giants -- not Dallas -- that might settle in their place.
But first the distractions have to be eradicated. The Burress situation will soon be settled with Burress maybe heading to Pittsburgh. But Umenyiora could be a problem. The Giants need to quickly deal with his situation. Letting that fester just wouldn't be smart. And the Giants are usually pretty smart because Reese is smart.
Now, if we could only keep Umenyiora from punching him in the grill.


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Happy Girls Day★Hinamatsuri Kimono Sushi Dolls
|Lotus root
|1 Medium, peeled, sliced
|1 Tablespoon
|Shitake mushrooms
|4 Medium, sliced
|1 Medium, chopped finely
|Snow pea
|10 Medium
|Kelp tea
|1 Teaspoon
|Soya sauce
|1 1⁄2 Tablespoon
|1 Tablespoon
|1 1⁄2 Tablespoon
|2 Tablespoon
|Quail eggs
|10 Small, hard boiled
|2 Large
|Salt and pepper
|To Taste
|Rice vinegar
|3 Tablespoon
|Cooked rice
|3 Cup (48 tbs)
|Sesame seed
|1 Tablespoon
|Sakura denbu
|1 Tablespoon (dried white fish)
|Ham slice
|1 Medium
|Dry sea weed
|1 Tablespoon
|Black sesame seed
|1 Teaspoon
1. Peel and slice lotus root, shitake mushroom, carrot and snow peas.
2. In a bowl, combine water and vinegar. Dip lotus root in it.
3. In a pot, heat water and plum kelp tea. Add soya sauce, sake, sugar and mirin.
4. Now, Add mushrooms, carrots and lotus roots. Cover the lid and simmer for 10 minutes.
5. Boil eggs in salted water.
6. In a bowl, whisk 2 eggs with salt and pepper.
7. In a pan, heat oil and pour the egg mixture. Cook it on both sides and flip it on the plate. Keep aside.
8. Check the simmering vegetables and add snow peas into it.
9. Drain quail eggs and remove shell.
10. Take out snow peas and slice it.
11. Keep two snow peas and carrot slices aside.
12. Add rice vinegar to the pot.
13. For making sushi rice - in a large pot combine sushi rice and vegetables.
14. Add snow peas to the rice.
15. Add sesame seed to the rice and let it cool.
16. Add sakura denbu to the rice.
17. On a saran wrap, put sushi rice and make a ball.
18. Cut egg sheet into two halves and wrap over sushi rice bowl. This makes kimono for dolls.
19. Fix half a slice of lotus root with a toothpick to make fan.
20. With a tooth pick fix an egg as doll's head.
21. With seaweed make empress long hair.
22. Make dolls eyes with sesame seeds.
23. Use carrot piece to make lips of doll.
24. Decorate it with carrot slice. Empress doll is ready.
25. For making emperor doll – using snow pea make sword of king and use seaweed to make his crown.
26. Serve the dolls with desired sauce.


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This 12-piece set includes everything you need to perfectly perform all the basic cooking tasks, large or small. From reheating a single portion of soup to preparing appetizers and entrées for the entire family, you'll find what you need here.


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1980 Maserati Merak '80 Maserati Merak in orange with black interior. Very nice color combination. Recent paint. Comes equipped with a 5 speed manual transmission,... More
1972 Maserati Bora 4.7 Coupé Here is an exciting find, this is a matching numbers European delivery Bora which was just recently discovered sitting in... More
This 2008 Maserati Quattroporte 4dr 2008 Maserati Quattroporte Sport GT S Sedan features a 4.2L 8 CYLINDER 8cyl Gasoline engine. It is equipped with... More
AM 101*1806 was, according to the Maserati Certificate of Origin, completed in September 1961. Originally White it was delivered ironically to a Signore Albino.... More
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In Detroit's Black neighborhoods, safety has been sacrificed to austerity.
Real solidarity will help to mobilize forces everywhere that are coming to realize the deepening costs and injustices of accepting capitalism's continuation.
Noam Chomsky's most recent book is Power Systems: Conversations on Global Democratic Uprisings and the New Challenges to U.S. Empire. Interviews with David Barsamian. Chomsky is emeritus professor of linguistics and philosophy at the Massachusetts Institute of Technology, in Cambridge.


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Migratory birds that breed in the Arctic are starting to nest earlier in spring because the snow melt is occurring earlier in the season.
This is confirmed by a new collaborative study, "Phenological advancement in arctic bird species: relative importance of snow melt and ecological factors," published in the current online edition of the journal Polar Biology. The scientists, including Wildlife Conservation SocietyE biologists, looked at the nests of four shorebird species and one songbird in Alaska, recording when the first eggs were laid in the nests. The work was undertaken across four sites ranging from the oil fields of Prudhoe Bay to the remote National Petroleum Reserve of western Arctic Alaska.
The scientists looked at nesting plots at different intervals in the early spring. Other variables, like the abundance of nest predators (which is thought to affect the timing of breeding) and satellite measurements of "greenup" (the seasonal flush of the new growth of vegetation) in the tundra were also assessed as potential drivers, but were found to be less important than snow melt.
Lead author Joe Liebezeit from the Audubon Society of Portland says "it seems clear that the timing of the snow melt in Arctic Alaska is the most important mechanism driving the earlier and earlier breeding dates we observed in the Arctic. The rates of advancement in earlier breeding are higher in Arctic birds than in other temperate bird species, and this accords with the fact that the Arctic climate is changing at twice the rate."
Morten Rasch from the Arctic Environment Dept of Aarhus University in Denmark is the coordinator of one of the most ambitious ecological monitoring programs in the Arctic. The Greenland Environment Monitoring Program includes two stations, Zackenberg, which is in the High Arctic region, and Nuuk, Greenland's capital, in the "lower" Arctic. Hansen and other members of Rasch's teams monitor 3,500 different parameters in a cross-disciplinary project, combining biology, geology, glaciology, all aspects of research into the fragile ecosystems of the Arctic. At that time, he told me during an interview, 10 years of monitoring had already come up with worrying results:
"We have experienced that temperature is increasing, we have experienced an increasing amount of extreme flooding events in the river, we have experienced that phenology of different species at the start-up of their growing season or the appearance of different insects for instance now comes at least 14 days earlier than when we started. And for some species, even one month earlier. And that's a lot. You have to realize the entire growing season in these areas is only three months. When we start up at Zackenberg in late May, or the beginning of June, the ecosystem is completely covered in snow and more or less frozen, and when we leave, in normal years -- or BEFORE climate change took over -- then we left around 1st September and the ecosystem actually started to freeze up. So the entire biological ecosystem only has three months to reproduce and so on. And in relation to that, a movement in the start of the system between 14 days and one month – that's a lot."
I can't write about birds and climate change in the Arctic without finishing off with a mention of George Divoky, an ornithologist whose bird-monitoring has actually turned into climate-change monitoring on Cooper Island, off the coast of Barrow, Alaska. George looks after a colony of black guillemots and spends his summer on the island. In recent years, he has taken to putting up bear-proof nest boxes for the birds, because polar bears increasingly come to visit, as the melting of the sea ice has reduced their hunting options. He has also observed the presence of new types of birds which did not previously come this far north.
This story is posted on Alaska Dispatch as part of Eye on the Arctic [1], a collaborative partnership between public and private circumpolar media organizations.