text:
8th October 2009
Trans woman goes to court after being denied breast implants 20
1:27 PM — A trans woman who was denied breast enlargement surgery is taking her primary care trust to court. The patient, known only as 'C', has been living as a woman for ten years and has undergone hormone treatment, although she has not had any other surgery.
French culture minister urged to resign over sex with boys 7
11:34 AM — Frederic Mitterrand, the French culture minister, is facing calls for his resignation after writing in a 2005 autobiography about paying boys for sex.
7th October 2009
Mary Cheney pregnant with second child 1
5:44 PM — Mary Cheney, the lesbian daughter of former US vice-president Dick Cheney, is pregnant with her second child. She had her first baby, Samuel David, in 2007.
Police release CCTV images of homophobic attack suspects 6
5:29 PM — Police have released images of two women they are seeking in connection with a homophobic attack in Trafalgar Square last month. A 62-year-old man is still in hospital with serious head injuries.
Stonewall chief Ben Summerskill says Tories are monitoring Polish allies' homophobia 15
4:39 PM — Stonewall chief executive Ben Summerskill has said he has been reassured by the Conservative Party that it is checking past statements made by its European allies for homophobia. He also denied he had been "used" by Labour when he pulled out of a gay Tory event last night.
First official gay Tory party hailed a success despite Stonewall boycott 21
3:37 PM — Spirit Bar in Canal Street was packed to its 650-person capacity last night as the Conservative Party held its first-ever official gay event. As the Tory-themed cocktails flowed, there was much speculation at the absence of Stonewall chief Ben Summerskill.
Christian council worker sues after being sacked for homophobic email 57
3:02 PM — A Christian council worker who was sacked for sending a homophobic email is suing her former employers. Denise Haye, 25, worked at Lewisham Council's legal services department.
Cameron: 'Polish allies are not homophobic' 59
2:17 PM — Tory leader David Cameron has said that his party's allies in Europe are not homophobic. Yesterday, an open letter from celebrities such as Stephen Fry, Eddie Izzard and Ewan MacGregor urged Cameron to oppose the views of the Polish Law and Justice Party.
Canadian gay man admits lying to donate blood 11
10:57 AM — A gay man in Canada is suing the the country's blood services for barring him from donating blood. Kyle Freeman admitted in court this week that he had lied about his sexual history in order to undergo the procedure, which he believes is the "ultimate gift" a person can give.
6th October 2009
Video: Stephen Fry says David Cameron has aligned himself with homophobes 33
7:06 PM — A letter signed by celebrities including Ewan MacGregor, Stephen Fry and Jo Brand has asked Tory leader David Cameron to oppose the views of his party's new European allies. Fry tonight told Channel 4 News that Mr Cameron had aligned his party with an "unpleasantly homophobic nationalistic party in Poland and sharing their whip in Europe."
Men on trial for murder of gay Wembley shopkeeper
4:45 PM — Two men have denied the murder of a gay Wembley shopkeeper who was found dead at his home last November. Dilip Unadkat, 48, died after he was stabbed five times in the chest in his flat in Alexandra Court, Empire Way.
Gay council chief accused of abusing authority over LGBT staff network 22
3:10 PM — An openly gay council leader in Aberdeen has been accused of abusing his authority to set up an LGBT staff network.
Stars complain over Tories' 'anti-gay' allies in Europe 16
2:54 PM — A letter signed by celebrities such as Ewan MacGregor, Stephen Fry and Jo Brand has asked Tory leader David Cameron to oppose the views of his party's new European allies.
Fugitive preying on gay men through dating websites 9
12:55 PM — Police have warned gay men and trans people in the Sussex area to be on their guard after a man believed to target gay and trans victims escaped from jail.
Gay Tory group's anger over 'Shame' nightclub 46
12:50 PM — LGBTory, a gay Conservative Party-affiliated group, has attacked a nightclub for hosting a 'Tory Shame' night on the same evening as the party holds a Pride night.
Russian court denies lesbians the right to marry
10:45 AM — A Russian lesbian couple have been barred from marrying by a court. The Tverskoi District Court ruled that Irina Fedotova-Fet and Irina Shipitko cannot marry as Russia law states marriage is between a man and a woman.
5th October 2009
Obama tipped to address gay rights dinner 7
6:23 PM — US president Barack Obama is reportedly planning to speak at a gay rights dinner on Saturday, the day before a gay rights march on Washington. He will attend the Human Rights Campaign fundraising dinner gala, a White House source told Associated Press.
Lesbians still being denied fertility treatment 4
6:21 PM — A Freedom of Information Act request has found that lesbian women are still being denied fertility treatment. According to a survey, primary care trusts appear to be turning down lesbian couples for treatment on the grounds that funding is only available for those with fertility problems.
Tory leader David Cameron challenged to commit to gay rights 24
2:48 PM — As the Conservative Party Conference gets underway in Manchester, party leader David Cameron has been called on to prove his commitment to gay rights.
Obama to tackle gay military ban 'at the right time' 15
2:02 PM — US president Barack Obama's national security adviser has said he will tackle the issue of the ban on gays and lesbians serving openly in the military "at the appropriate time".


text:
MELBOURNE, Fla. - Barry women's tennis player Linda Fritschken has been named the 2014-15 Dr. M. Eileen McDonough Sunshine State Conference Female Scholar-Athlete of the Year. This is the ninth instance in which a Buccaneer athlete has won the award since the first was given out in 1995-96. Read the full story.
MELBOURNE, Fla. - With the conclusion of the 2015 tennis season, the final Intercollegiate Tennis Association (ITA) tennis rankings were released. Five men's teams and six women's teams from the Sunshine State Conference were included in the polls that were released on May 27th. Read the full story.
SKILLMAN, N.J. - The Intercollegiate Tennis Association announced its 2015 ITA All-American Teams for Women's Division II. The Sunshine State Conference received eight total All-American honorees. Read the full story.
SURPRISE, Ariz. -- Barry University women's tennis lost to Armstrong Atlantic, 5-2, in the NCAA Division II Championship match Saturday night. Read the full story.
SURPRISE, Ariz. – Barry University women's tennis has been delayed by weather several times throughout the season. Read the full story.

subset: commoncrawl


text:
If your little kid has to wear an eyepatch, maybe you can take a little inspiration from these awesome parents who customized their baby's patch every day and made her look super cool.Read Full Story
Music created for kids falls into one of two categories: the teeth-achingly simpy or the headache-inducing covers of pop songs rehashed for the diaper set (Kidz Bop, anyone?). Seattle musician and composer Eli Rosenblatt instead creates empowering, fun, heartfelt music for kids that ignites their imaginations. He tells us what inspires him to do this.Read Full Story
Toddlers aren't exactly known for their enduring patience and ability to sit still for long periods of time, which is why you take your sanity into your own hands when you attempt to cut their hair yourself.Read Full Story
Is your child the one that other kids find annoying because he's too young, too rough, too loud? Many of my family's and friends' kids are older than my 2-year-old son, which can make for un-relaxed socializing for all concerned.Read Full Story
And you'll see personalized content just for you whenever you click the My Feed .
SheKnows is making some changes!


text:
Tabletop & Bar
- Monogrammed Hotel Silver Flatware Place Setting Sugg. Price: $119.95 – $429.95 Sale $94.99 – $379.99
Related Products
Williams-Sonoma Estate Champagne Wine Glasses, Set of 2
Williams-Sonoma Estate Champagne Wine Glasses, Set of 2
Since 1956, innovation, quality and craftsmanship have been part of the Williams Sonoma legacy. Our signature Estate Champagne flutes embody that philosophy in their sleek lines, beautiful proportions and varietal enhancing bowls. Refined and versatile, the glasses are ideal ...


text:
OAKLAND (AP) - A judge said Friday that the mother of a 13-year-old girl who was declared brain dead after tonsil surgery can remove her daughter from a California hospital if she assumes full responsibility for the consequences.
JUDGE SAYS SF COMMUNITY COLLEGE CAN REMAIN ACCREDITED: SAN FRANCISCO (AP) - A San Francisco judge has ruled that a private commission can't revoke the accreditation of one of the nation's largest community colleges until a trial can be held to determine if the action is lawful.
LOS ANGELES (AP) - The Porsche carrying "Fast & Furious" star Paul Walker may have been going 100 mph or more before it crashed, killing both Walker and the driver, according to a coroner's report released Friday.
SAN FRANCISCO (AP) - A man who illegally came to the United States two decades ago said he hopes a court ruling granting him a law license will open doors to millions of other immigrants in the same situation.
SAN FRANCISCO (AP) - California's $324 million Mega Millions jackpot winner Steve Tran had a 3 a.m. epiphany earlier this week that spurred him from sleep and had him fumbling through a pile of lottery tickets on top of his dresser.
MURIETTA (AP) - A Southern California man has been arrested after his 3-year-old Akita allegedly attacked a toddler at a home improvement store.
EUREKA . (AP) - When the body of the Rev. Eric Freed was discovered in the rectory of his church on New Year's Day, police immediately had a suspect: A man released from jail hours earlier who was spotted on church grounds by a security guard, questioned by a police officer, and then seen again by the same guard, this time holding a wooden stake.
SACRAMENTO (AP) - The first Sierra snow survey of the winter on Friday confirmed the fears of state water managers, who are warning of drought conditions in the coming year unless the state receives significantly more rain and snow.
LOS ANGELES (AP) - A German architect pleaded no contest to involuntary manslaughter Friday in the death of a Los Angeles firefighter crushed in the collapse of a ceiling during a fire at a luxury home the architect designed and built in the Hollywood Hills.
OAKLAND (AP) - A federal judge on Thursday ordered settlement talks between lawyers for a California hospital and a 13-year-old California girl declared brain dead after tonsil surgery.
EUREKA (AP) - Police arrested a suspect Thursday in the killing of a respected priest and educator who was found dead on New Year's Day in a church rectory in Northern California.
WOODLAND (AP) - A former California Highway Patrol dispatcher and her husband are facing various charges after authorities say they orchestrated deals for drugs and weapons in Yolo County.
WOMAN KILLED, BABY INJURED CROSSING FREMONT STREET: FREMONT (AP) - A 19-year-old woman has been killed and a 3-month-old boy she was carry has life-threatening injuries after they were struck by a vehicle on New Year's Eve in Fremont.
SACRAMENTO (AP) - Officials overseeing California's $68 billion high-speed rail project have taken pains in recent weeks to assure the public that construction plans are moving ahead, characterizing a series of recent setbacks as "a bump in the road."
SAN FRANCISCO (AP) - The California Supreme Court granted a law license Thursday to a man who has lived in the U.S. illegally for two decades, a ruling that advocates hope will open the door to millions of immigrants seeking to enter other professions such as medicine, accounting and teaching.
Page 207 of 540
LOS ANGELES (AP) - A newspaper analysis has found that the most recent estimate of the cost to ride California's bullet train from Los Angeles to San Francisco is a bargain at an average of $86.
MODESTO (AP) - A nursery near Modesto sold pistachio trees with a disorder that officials say could affect as many as 20,000 acres of San Joaquin Valley orchards.
LOS ANGELES (AP) - Los Angeles Mayor Eric Garcetti has signed off on an agreement to clean polluted storm runoff water and redirect it to the city's groundwater basin.
SAN FRANCISCO (AP) - San Francisco has become the first city in the nation to outlaw chewing tobacco from its playing fields, including AT&T Park, home to the San Francisco Giants.
SAN DIEGO (AP) - The City of San Diego has confirmed that despite the drought, they are pumping millions of gallons of drinking water into Chollas Lake for recreational fishing.
Page 19 of 19
Contents of this site are © 2015 Manteca Bulletin - All Rights Reserved
Privacy policy and Terms of service | Contact Us


text:
Justin I. Hale was recently named as shareholder for the law firm of Carr Allison. Hale's practice includes defense of personal injury, premises liability, product liability, transportation, and workers' compensation matters.
Sign up to receive Birmingham Business Journal's Afternoon Edition (featuring People on the Move and the latest local business news), Morning Edition newsletters and breaking news alerts.
Subscribe now to search our database of 6626 Birmingham Business Journal People on the Move submissions and download their contact information.
© 2015 American City Business Journals. All rights reserved. Use of this Site constitutes acceptance of our User Agreement (updated 3/14/12) and Privacy Policy (updated 3/14/12).
Your California Privacy Rights.
The material on this site may not be reproduced, distributed, transmitted, cached or otherwise used, except with the prior written permission of American City Business Journals.


text:
Gunmetal Black Ep 01
episode 14.23 / 5.00 3,366 Views
Lucina's First Day
A look at the first day of Lucina in Super Smash4.22 / 5.00 10,160 Views
BATMAN v SUPERMAN final showdown
SPOILERS!!4.15 / 5.00 9,428 Views
This i a very fine animation it has lots of funny stuff like the angel part that was"LULZ"
overall it was fun
but i think it will br cool if you put some random caracter that gets hit or something or a dancing part
Tip* make a catch frase
thankyou for the review and i am taking that tip thank you lol
nice concider...
some subtitles would be great for this...
at some parts you can`t understand what they are saying....
I`m waiting for the other 4 parts XD
glad to know :D thanks
Have fun angel!
Hahahahaha This is funny, but i wonder why this isn't rated mature because of sex jokes?
there not really that graphic. thanks for the review :D
It was above average!
I liked everything except for the killing part with the silhouettes. It was just a bit too random and drawn out. Everything else was hilarious though.
thank you :D
Overall good
As said it was really good and fuun! :3, but (don't take this to hard might just be me) You talk very fast and I hard to be able to hear what you say. So recomendation is that you have subtitles. But again it's just might be me.
nah i do i talk fast it's haunted me my whole life but thank you very much :)


text:
Visit our other Wisconsin Historical Society websites!
Refine or narrow your results by clicking any term in the gray boxes below.
Expand your results by removing any breadcrumb in a gray outlined box above. Click on the blue "X" to remove the term.
Narrow your results by entering a keyword into the search box above. Then click the "Search Within" checkbox, then click on the orange "Search" button.
If you did not find what you are looking for or you did not get any results, try changing your search term(s). See our Search Help page for more tips.
Date: 1948
Description: Prior to the 1948 State Fair, artist Robert Hodgell traveled Wisconsin to study its many landscapes. He then produced a series of study sketches outlining...
Date: 1932
Description: Diorama(?) depicting the test of the first mechanical reaper by Cyrus McCormick at Steele's Tavern, Virginia in 1831. The display was likely part of Intern...
Date: 1859-12-02 ca.
Description: Business with Frank Leslie's Illustrated Newspaper in New York took Hölzlhuber east where he also visited Philadelphia, Baltimore, and Washington. H...
Date: 1886 ca.
Description: Oil on linen study of a dead or wounded Civil War soldier created as a preliminary study by one of the Milwaukee panorama painters active during the 1889s....
Date: 1960
Description: Pencil, ink, gouache, and watercolor design of a black skirt and brown tweed jacket over a red pullover for Audrey Hepburn in "Breakfast at Tiffany's" (Par...
Date: 1939 ca.
Description: The Leitch house located at 752 E. Gorham Street.
Date: 1937
Description: Boyhood home of Hamlin Garland.
Date: 1953
Description: Pencil, ink, watercolor, and gouache design for a black, off the shoulder evening gown for Martha Hyer to wear in "Sabrina" (Paramount 1954). She wears whi...
Date: 1968
Description: Cover for the Wisconsin Friends of Our Native Landscape program. Depicted are two geese and a duck on a lake. The Wisconsin Friends chapter was founded in...
Description: Portrait of Chief Buffalo, over-painted enlargement, possibly from a double-portrait (possibly an ambrotype). Grandson of Great Chief Buffalo. Chief Buffa...
Description: Portrait of Daniel Bread wearing a suit.
Date: 1938 ca.
Description: The George Storer house, 104 East Gilman Street, built in 1855 by John E. Kendall.
Date: date unknown
Description: An Ojibwa man and woman prepare to transport rolled bark back to their home to use in the construction of a canoe.
Description: Sioux on horseback charging with weapons raised.
Date: 1998
Description: This scene was painted by Seferina Contreras Klinger from memories of her grandmother, Aurelia M. Contreras, and her uncle, Fidel Contreras, who worked pic...
Description: The sandstone house known as Walker's Castle was located in the 900 block of East Gorham Street. According to newspaper articles, it was built in about 186...
Date: 1972
Description: Cover for the Wisconsin Friends of Our Native Landscape program. Depicted is a red wing blackbird perching on cattail flowers in a body of water. The Wisco...
Date: 1900 ca.
Description: Oil on paper painting of a butterfly. Bierstadt would apply oil paints directly to the paper to form one wing of a butterfly, then fold the paper in Rorsch...
Date: 1961
Description: Pencil, ink, gouache, and watercolor design for a blue and white polka dot romper with drawstrings at the waist and shoelace straps for Audrey Hepburn's "s...
Description: Portrait of John Starkweather.
If you didn't find the material you searched for, our Library Reference Staff can help.
Call our reference desk at 608-264-6535 or email us at:
© 1996-2015 Wisconsin Historical Society, 816 State Street, Madison, WI 53706


text:
Journal of Immunological Methods (J IMMUNOL METHODS)
Journal description
The Journal of Immunological Methods is devoted to covering techniques for: (1) Quantitating and detecting antibodies and/or antigens and haptens based on antigen-antibody interactions. (2) Fractionating and purifying immunoglobulins, lymphokines and other molecules of the immune system. (3) Isolating antigens and other substances important in immunological processes. (4) Labelling antigens and antibodies with radioactive and other markers. (5) Localizing antigens and/or antibodies in tissues and cells, in vivo or in vitro. (6) Detecting, enumerating and fractionating immunocompetent cells. (7) Assaying for cellular immunity. (8) Detecting cell-surface antigens by cell-cell interactions. (9) Initiating immunity and unresponsiveness. (10) Transplanting tissues. (11) Studying items closely related to immunity such as complement, reticuloendothelial system and others. In addition the journal will publish articles on novel methods for analysing the organization, structure and expression of genes for immunologically important molecules such as immunoglobulins, T cell receptors and accessory molecules involved in antigen recognition, processing and presentation. Submitted full length manuscripts should describe new methods of broad applicability to immunology and not simply the application of an established method to a particular substance - although papers describing such applications may be considered for publication as a short Technical Note The Recombinant Technology section will contain articles relating to modification by recombinant techniques of molecules of immunological interest; isolation of novel binding proteins by phage display; gene therapy; transfection; and expression. Immunology Protocols is a section providing detailed, step-by-step descriptions of new and established techniques in immunology. Articles on the molecular biological analysis of immunologically relevant receptor binding sites are also invited.
Current impact factor: 2.01
Impact Factor Rankings
|2015 Impact Factor
|Available summer 2015
|2013 / 2014 Impact Factor
|2012 Impact Factor
|2011 Impact Factor
|2010 Impact Factor
|2009 Impact Factor
|2008 Impact Factor
|2007 Impact Factor
|2006 Impact Factor
|2005 Impact Factor
|2004 Impact Factor
|2003 Impact Factor
|2002 Impact Factor
|2001 Impact Factor
|2000 Impact Factor
|1999 Impact Factor
|1998 Impact Factor
|1997 Impact Factor
|1996 Impact Factor
|1995 Impact Factor
|1994 Impact Factor
|1993 Impact Factor
|1992 Impact Factor
Impact factor over time
Additional details
|5-year impact
|Cited half-life
|Immediacy index
|Article influence
|Journal of Immunological Methods website
|Other titles
|Journal of immunological methods, Immunological methods, JIM
|Material type
|Periodical, Internet resource
|Document type
|Journal / Magazine / Newspaper, Internet Resource
Publisher details
- Pre-print
- Author can archive a pre-print version
- Post-print
- Author can archive a post-print version
- Conditions
- Pre-print allowed on any website or open access repository
- Voluntary deposit by author of authors post-print allowed on authors' personal website, arXiv.org or institutions open scholarly website including Institutional Repository, without embargo, where there is not a policy or mandate
- Deposit due to Funding Body, Institutional and Governmental policy or mandate only allowed where separate agreement between repository and the publisher exists.
- Permitted deposit due to Funding Body, Institutional and Governmental policy or mandate, may be required to comply with embargo periods of 12 months to 48 months .
- Set statement to accompany deposit
- Published source must be acknowledged
- Must link to journal home page or articles' DOI
- Publisher's version/PDF cannot be used
- Articles in some journals can be made Open Access on payment of additional charge
- NIH Authors articles will be submitted to PubMed Central after 12 months
- Publisher last contacted on 18/10/2013
- Classification green
Publications in this journal
- SourceAvailable from: sciencedirect.com
Article: Corrigendum to "The pre-analytical processing of blood samples for detecting biomarkers on protein microarrays" [Journal of Immunological Methods volume 418 (2015) 39–51]Journal of Immunological Methods 04/2015; 419. DOI:10.1016/j.jim.2015.04.003
Article: Evaluation of the efficiency of human immune system reconstitution in NSG mice and NSG mice containing a human HLA.A2 transgene using hematopoietic stem cells purified from different sources[Show abstract] [Hide abstract]
ABSTRACT: Severely immunodeficient mice such as the NOD/SCID/IL2rγ(null) (NSG) strain can be engrafted with human hematopoietic stem cells (HSCs), resulting in chimeric mice containing many components of the human immune system (Human Immune System mice or HIS mice). HIS mice can both support the replication of and recapitulate much of the immunological response to a variety of pathogens, including ones with strict human tropism, such as HIV-1. In an effort to develop a better mouse model for human infectious pathogen infection and possible immune resolution, we compared the human immune system reconstitution of NSG mice following injection with human CD34+ HSCs purified from either fetal liver (FL) or umbilical cord blood (UCB). We analyzed reconstitution in standard NSG mice as well as a derivative of these mice containing an HLA.A2 encoding transgene (NSG.A2). HSCs from both sources effectively reconstituted hematopoietic lineages when injected into NSG mice. In marked contrast, total CD45(+) human hematopoietic cells in NSG.A2 mice were well reconstituted by HSCs from UCB but very poorly by HSCs purified from FL. Moreover, the reconstitution of T cell lineages in NSG.A2 mice by HSCs from UCB was inferior to that obtained using NSG mice. We also found that FL CD34(+) HSCs contain a much higher percentage of cells with a phenotype consistent with primitive progenitors than UCB HSCs. We discuss possible explanations for the influence of the HLA.A2 transgene on hematopoietic reconstitution using the two sources of HSCs. Copyright © 2015. Published by Elsevier B.V.Journal of Immunological Methods 03/2015; 422. DOI:10.1016/j.jim.2015.02.007
Article: Sensitive recovery of recombinant antibody clones after their in silico identification within NGS datasets[Show abstract] [Hide abstract]
ABSTRACT: Recently the analytical power of the latest high throughput next generation DNA sequencing platforms has been used to analyse phage that have been selected from the panning of large combinatorial libraries displaying either peptide or antibody ligands. This process, commonly referred to as next generation phage display (NGPD), allows the researcher to determine the identity of specific phage that are being enriched against an antigen target by analysis of the DNA sequence encoding the displayed ligand. This method bypasses several steps in conventional phage panning that include laborious colony picking and functional ligand screening. A downside of this approach is that the only output from such experiments is the DNA sequence information of such enriched phage particles. In the case of peptides, the peptide sequence can be synthesised directly and used for further screening, however this is more difficult with larger antibody fragments such as ScFvs. In the case of ScFvs, their coding sequence would have to be fully elucidated, synthesised and re-cloned before expression. We describe here the application of an inverse PCR-ligation methodology that enables the specific recovery of ScFvs of interest from enriched sub-libraries of phage clones. Phagemid particles are recovered using sequence information derived from their unique heavy chain CDR3/FR4 domains and specific clones can be recovered irrespective of CDR3 size and at levels of abundance that would be refractory to their discovery during conventional phage panning and screening. Copyright © 2015. Published by Elsevier B.V.Journal of Immunological Methods 03/2015; 420. DOI:10.1016/j.jim.2015.03.005
Article: Preferential recognition of isocitrate dehydrogenase by a rabbit monoclonal antibody (ab124797) against the C-terminal peptide of RANKL[Show abstract] [Hide abstract]
ABSTRACT: A rabbit monoclonal antibody (Abcam ab124797), with high affinity for a synthetic peptide corresponding to the C-terminal region of the receptor activator of nuclear factor (NF)-κB ligand (RANKL), specifically recognizes a 37 kDa protein by immunoblotting, in good agreement with the molecular mass of RANKL. However, our mass spectroscopy analysis revealed that the protein recognized by the antibody is the α-subunit of NAD(+)-dependent isocitrate dehydrogenase (ICDH), a key Krebs cycle enzyme in mitochondria. Consistently, immunocytochemical staining with the antibody revealed a network organization characteristic of mitochondria, which overlapped with staining by MitoTracker and was lost after the siRNA-mediated downregulation of ICDH. The C-terminal peptide of ICDH contains similar chemical characteristics to that of the RANKL peptide and interacts with the antibody, although the affinity is a hundred times weaker. The present study provides an example of the preferential recognition of a surrogate protein by a rabbit monoclonal antibody. Copyright © 2015. Published by Elsevier B.V.Journal of Immunological Methods 03/2015; 420. DOI:10.1016/j.jim.2015.03.006
- [Show abstract] [Hide abstract]
ABSTRACT: The study aimed to evaluate cell surface mobilisation of CD107a as a general activation marker on chicken cytotoxic T cells (CTL). Experiments comprised establishment of an in vitro model for activation induced CD107a mobilisation and design of a marker panel for detection of CD107a mobilisation on chicken CTL isolated from different tissues. Moreover, CD107a mobilisation was analysed on CTL isolated from airways of infectious bronchitis virus (IBV) infected birds direct ex vivo and upon in vitro stimulation. Results showed that phorbol 12-myristate 13-acetate (PMA) in combination with ionomycin was a consistent inducer of CD107a cell surface mobilisation on chicken CTL in a 4h cell culture model. In chickens experimentally infected with IBV, a higher frequency of CTL isolated from respiratory tissues were positive for CD107a on the cell surface compared to those from uninfected control chickens indicating in vivo activation. Moreover, upon in vitro PMA+ionomycin stimulation higher proportions of CTL isolated from the airways of IBV infected chickens showed CD107a mobilisation compared to those from uninfected control chickens. Monitoring of CD107a cell surface mobilisation may thus be a useful tool for studies of chicken CTL cytolytic potential both in vivo and in vitro. Copyright © 2015. Published by Elsevier B.V.Journal of Immunological Methods 03/2015; 419. DOI:10.1016/j.jim.2015.02.011
- [Show abstract] [Hide abstract]
ABSTRACT: Cut points in immunogenicity assays are used to classify future specimens into anti-drug antibody (ADA) positive or negative. To determine a cut point during pre-study validation, drug-naive specimens are often analyzed on multiple microtiter plates taking sources of future variability into account, such as runs, days, analysts, gender, drug-spiked and the biological variability of un-spiked specimens themselves. Five phenomena may complicate the statistical cut point estimation: i) drug-naive specimens may contain already ADA-positives or lead to signals that erroneously appear to be ADA-positive, ii) mean differences between plates may remain after normalization of observations by negative control means, iii) experimental designs may contain several factors in a crossed or hierarchical structure, iv) low sample sizes in such complex designs lead to low power for pre-tests on distribution, outliers and variance structure, and v) the choice between normal and log-normal distribution has a serious impact on the cut point. We discuss statistical approaches to account for these complex data: i) mixture models, which can be used to analyze sets of specimens containing an unknown, possibly larger proportion of ADA-positive specimens, ii) random effects models, followed by the estimation of prediction intervals, which provide cut points while accounting for several factors, and iii) diagnostic plots, which allow the post hoc assessment of model assumptions. All methods discussed are available in the corresponding R add-on package mixADA. Copyright © 2015 Elsevier B.V. All rights reserved.Journal of Immunological Methods 02/2015; 418. DOI:10.1016/j.jim.2015.02.004
Article: Analysis of CCR7 mediated T cell transfectant migration using a microfluidic gradient generator[Show abstract] [Hide abstract]
ABSTRACT: T lymphocyte migration is crucial for adaptive immunity. Manipulation of signaling molecules controlling cell migration combined with in-vitro cell migration analysis provides a powerful research approach. Microfluidic devices, which can precisely configure chemoattractant gradients and allow quantitative single cell analysis, have been increasingly applied to cell migration and chemotaxis studies. However, there are a very limited number of published studies involving microfluidic migration analysis of genetically manipulated immune cells. In this study, we describe a simple microfluidic method for quantitative analysis of T cells expressing transfected chemokine receptors and other cell migration signaling probes. Using this method, we demonstrated chemotaxis of Jurkat transfectants expressing wild-type or C-terminus mutated CCR7 within a gradient of chemokine CCL19, and characterized the difference in transfectant migration mediated by wild-type and mutant CCR7. The EGFP-tagged CCR7 allows identification of CCR7-expressing transfectants in cell migration analysis and microscopy assessment of CCR7 dynamics. Collectively, our study demonstrated the effective use of the microfluidic method for studying CCR7 mediated T cell transfectant migration. We envision this developed method will provide a useful platform to functionally test various signaling mechanisms at the cell migration level. Copyright © 2015. Published by Elsevier B.V.Journal of Immunological Methods 02/2015; 419. DOI:10.1016/j.jim.2015.02.008
Article: Development and Characterization of a Free Therapeutic Ligand Binding Assay with Assistance from Kinetics Modeling.[Show abstract] [Hide abstract]
ABSTRACT: Bioanalytical data from early human studies conducted in normal volunteers are often used for building pharmacokinetic/pharmacodynamic models that can predict outcomes of future studies in diseased patients. Thus, it's important to develop and validate reliable and accurate bioanalytical assays that instill confidence that the intended therapeutic species (total or free) are being measured. Assays quantifying the free therapeutic species, the partially bound (for multivalent therapeutics) and unbound species, require much more characterization than assays that quantify the total therapeutic species. We have developed an immunoassay to measure free BMS-962476, an Adnectin protein therapeutic against soluble proprotein convertase subtilisin kexin (PCSK)-9, and performed an in-depth characterization of the accuracy of this assay with the assistance of modeling. The experimental data correlates with modeled data within 15% at all clinically relevant levels of PCSK9 in normal and diseased populations. Copyright © 2015. Published by Elsevier B.V.Journal of Immunological Methods 02/2015; 419. DOI:10.1016/j.jim.2015.02.009
Article: Citrobacter rodentium-induced colitis: A robust model to study mucosal immune responses in the gut[Show abstract] [Hide abstract]
ABSTRACT: Citrobacter rodentium is a natural mouse pathogen which reproducibly infects mice and causes intestinal disease. The C. rodentium model of infection is very useful for investigating host-pathogen immune interactions in the gut, and can also be used to understand the pathogenesis of several important human intestinal disorders, including Crohn's disease, ulcerative colitis, dysbiosis and colon tumorigenesis. Both innate and adaptive immune responses play a critical role in protection against C. rodentium. Here, we summarize the role of immune components in protection against C. rodentium and describe techniques for the analysis of innate and adaptive mucosal immune responses, including setting up the infection, analysis of colonic hyperplasia and bacterial dissemination, evaluation of antibody responses, and purification and analysis of intestinal epithelial and lymphoid cells. Copyright © 2015. Published by Elsevier B.V.Journal of Immunological Methods 02/2015; 421. DOI:10.1016/j.jim.2015.02.003
Article: The novel multispecies Fc-specific Pseudomonas exotoxin A fusion protein α-Fc-ETA′ enables screening of antibodies for immunotoxin development[Show abstract] [Hide abstract]
ABSTRACT: Immunoconjugates that deliver cytotoxic payloads to cancer cells represent a promising class of therapeutic agents which are intensively investigated in various clinical applications. Prerequisites for the generation of effective immunoconjugates are antibodies which efficiently deliver the respective cytotoxic payload. To facilitate the selection of human or mouse antibodies that display favorable characteristics as immunotoxins, we developed a novel Pseudomonas exotoxin A (ETA)-based screening protein. The α-Fc-ETA' consists of a multispecies-specific Fc-binding domain antibody genetically fused to a truncated ETA version (ETA'). α-Fc-ETA' non-covalently bound to human and mouse antibodies but did not form immune complexes with bovine immunoglobulins. In combination with antibodies harboring human or mouse Fc domains α-Fc-ETA' inhibited proliferation of antigen-expressing tumor cells. The cytotoxic effects were strictly antibody dependent and were observed with low α-Fc-ETA' concentrations. Mouse antibodies directed against CD7 and CD317/HM1.24 that previously had been used for the generation of functional recombinant immunotoxins, also showed activity in combination with α-Fc-ETA' by inhibiting growth of antigen-positive myeloma and leukemia cell lines. In contrast, α-kappa-ETA', a similarly designed human kappa light chain-specific fusion protein, was only specifically active in combination with antibodies containing a human kappa light chain. Thus, the novel α-Fc-ETA' fusion protein is broadly applicable in screening antibodies and Fc-containing antibody derivatives from different species to select for candidates with favorable characteristics for immunotoxin development. Copyright © 2015. Published by Elsevier B.V.Journal of Immunological Methods 02/2015; DOI:10.1016/j.jim.2015.02.002
Article: The pre-analytical processing of blood samples for detecting biomarkers on protein microarrays[Show abstract] [Hide abstract]
ABSTRACT: Specimen collection method and quality insurance is pivotal in biomarker discovery. Pre-analytical variables concerning blood collection and sample handling might affect analytical results and should be standardised prior application. In this study, we examine pre-analytical characteristics of blood samples using protein microarray. The influences of 1) standby times until centrifugation (1h, 4h, 24h and 48h), 2) four blood collection methods, and 3) IgG purified from those samples on differentially reactive antigens between samples ("DIRAGs") were investigated. Spearman correlation analyses of intra-individual arrays demonstrated remarkable differences (0.75-0.98 vs. 0.5-0.75) of antibody reactivities within and between serum and plasma samples. Class comparison showed that reactive antigen profiles were best preserved using IgG purified samples of serum tubes without separation gel as after 24hours 83% of the 1h baseline DIRAGs were re-found. Testing dilution series with protein microarrays and Luminex xMap® Technology, we found linear correlations (R(2)=0.94-0.99) between IgG concentration and read-out when using purified IgG instead of serum. Therefore, we highly recommend standardising pre-analytics and proposing the use of purified IgG for autoantibody immune-profiling with protein microarrays to reduce potential unspecific binding of matrix proteins abundant in serum and plasma samples. Although purified IgG cannot completely compensate the influence of pre-analytics, in highly parallel immune-profiling IgG enables reduction of unspecific effects, which occur when using serum or plasma for analysis on protein microarrays. Reproducibility problems due to pre-analytical processing of blood samples might explain discrepant results reported in various biomarker studies. Copyright © 2015 Elsevier B.V. All rights reserved.Journal of Immunological Methods 02/2015; 418. DOI:10.1016/j.jim.2015.01.009
Article: Amplification of highly mutated human Ig lambda light chains from an HIV-1 infected patient[Show abstract] [Hide abstract]
ABSTRACT: Isolation and characterization of anti HIV-1 broadly neutralizing antibodies (bNAbs) has elucidated new epitopes and sites of viral vulnerability. Anti-HIV-1 bNAbs typically show high levels of somatic mutations in their variable region genes. This feature potentially limits antibody identification, since the mutated antibody sequences are no longer complimentary to primers designed based on germline antibody sequences. Here we report a new set of primers for Igλ light chains that aligns to the 5' end of the leader sequence and is highly efficient for the amplification of antibodies that contain mutations and deletions in the 5' end of human Igλ. Copyright © 2015 Elsevier B.V. All rights reserved.Journal of Immunological Methods 02/2015; 418. DOI:10.1016/j.jim.2015.01.011
Article: Establishment of immunoassay for platelet-derived soluble glycoprotein VI, a novel platelet marker[Show abstract] [Hide abstract]
ABSTRACT: Soluble Glycoprotein VI (GPVI) is an attractive marker for disorders marked by platelet activation, such as thrombotic microangiopathy, myocardial infarction, and stroke. Several groups have already developed an immunoassay for soluble GPVI; however, there are several discrepancies between the groups' assays. In this study, we prepared the two types of recombinant soluble GPVI, the monomeric form GPVI (GPVI-His) and the dimeric form of GPVI (GPVI-Fc), moreover, we generated four anti-GPVI antibodies, F1232-7-1 (7S1), F1232-10-2 (10S2), F1232-19-1 (19D1), and F1232-21-1 (21D1). The former 2 antibodies (7S1 and 10S2) had a high affinity for both GPVI-His and GPVI-Fc, while the latter 2 antibodies (19D1 and 21D1) showed a high affinity for GPVI-Fc but low affinity for GPVI-His. All of the antibodies comparably recognized surface GPVI on resting platelets. Furthermore, we established two immunoassays for soluble GPVI, 7S1/10S2-HRP and 19D1/21D1-HRP (capture antibody/detection antibody). 7S1/10S2-HRP showed equivalent reactivity with GPVI-His and GPVI-Fc, whereas 19D1/21D1-HRP had high affinity for GPVI-Fc but low reactivity with GPVI-His. In terms of reactivity with platelet-derived soluble GPVI, 7S1/10S2-HRP demonstrated sensitive detection whereas 19D1/21D1-HRP was nonreactive. Taken together, 7S1/10S2-HRP is a suitable candidate for a reliable soluble GPVI immunoassay as it has a high affinity for monomeric GPVI.Journal of Immunological Methods 02/2015; 418. DOI:10.1016/j.jim.2015.01.010
Data provided are for informational purposes only. Although carefully collected, accuracy cannot be guaranteed. The impact factor represents a rough estimation of the journal's impact factor and does not reflect the actual current impact factor. Publisher conditions are provided by RoMEO. Differing provisions from the publisher's actual policy or licence agreement may be applicable.


text:
Police Beat: Sunday, Sept. 15, 2013
Man leads deputies on car chase in Collier County
A Lehigh Acres man was arrested Friday after Collier County sheriff's deputies say he led them on a high-speed car chase.
Jeffrey Eprhaim Ramos, 31, of the 3900 block of 32nd Street, faces multiple charges, including one for attempting to elude a law enforcement officer and driving with a suspended license.
The chase, which lasted about four minutes, began near Santa Barbara Boulevard and 19th Avenue and ended more than three miles away, in front of the Island Walk gated community on Vanderbilt Beach Road, the arrest report stated.
A deputy in an unmarked car noticed stolen plates on a beige Cadillac that Ramos was driving south on Santa Barbara Boulevard. Before the deputy could pull him over, Ramos made a U-turn that caused other drivers to hit their brakes to avoid hitting the Cadillac, deputies reported.
When the deputy activated his emergency lights, Ramos kept driving north, reached speeds of about 70 mph, ran a red light and put the Cadillac in the way of oncoming traffic multiple times, the report said.
Eventually, Ramos abandoned the car on Vanderbilt Beach Road and unsuccessfully tried to climb over the Island Walk community's wall, which is when deputies made the arrest, according to the report.
DUI arrests
Mycalann Alannah Deason, 22, of the 1300 block of Verde Drive, East Naples, was arrested Friday by Collier deputies at 2310 Pine Ridge Road.
Douglas Keating, 32, of the 800 block of Fifth Avenue South, Naples, was arrested Saturday by Collier deputies near U.S. 41 East and Bayshore Road.
Robyn Lee Jones, 36, of the 3300 block of Guilford Court, East Naples, was arrested Saturday by Collier deputies near U.S. 41 East and Lakewood Drive.
Domestic violence arrests
Michinique Dorilus, 21, of the 3700 block of Central Avenue, Fort Myers, was arrested Saturday by Collier deputies in the 5200 block of 19th Avenue. He is accused of slapping and trying to choke a woman.
Michael Francis Affleck, 25, of the 3200 block of 29th Avenue Southwest, Golden Gate, was arrested Saturday by Collier deputies at his home. He is accused of striking as woman's face several times with a Popsicle.
Grand theft arrests
Dennis Riley, 51, of West Roxbury, Mass., was arrested Saturday by Marco Island police at 1089 North Collier Boulevard. He is accused of trying to steal a framed sports jersey from the wall of a restaurant.
Donna M. Hall, 56, of Bluebill Avenue, North Naples, was arrested Friday by Collier deputies at 3701 U.S. 41 East. She is accused of trying to fraudulently refund items at a department store.
Burglary arrest
Hisam Abdel Wahab, 27, of Pittsburgh, was arrested Saturday by Collier deputies in the 500 block of 105th Avenue. He was found under a quilt in a bed in the home authorities allege he was burglarizing. He faces an additional charge of criminal mischief.
Subscribe to the Naples Daily News
Need Help? Call us at 1-888-878-6842.
Monday-Friday: 6am-6pm / Saturday: 7am-3pm / Sunday: 7am-2pm


text:
Division of University Advancement
The Division of University Advancement (UAD) supports the mission of the university by identifying, cultivating and securing resources to enhance and improve educational opportunities for students while serving the extended community.
The prioritization and recovery review began to move forward in August 2005, upon the completion of search for a new vice president. As UAD is a division designed to support the academic mission, it is essential that any long term plan is synergized with Academic Affairs.
Consultation Process
The division vice president met individually with every staff member to address short and long term goals. Those interviews came together as discussion points with division leaders that included: Ron Simons, Ron Fremont, Aida Morad, Sharon Joyce, Nikki Khurana, Ken Bonner, Melissa Riordan, Pamela Allen, Francine Ramirez and the development directors. The vice president conceptualized the outcomes with senior leadership and addressed the impact of the division priorities with all impacted managers.
Outcomes to Date
UAD receives approximately 2 percent of the General Fund budget, so the initial process has been one of efficiency and redeployment. UAD consists of 45 staff, which includes development officers for every college and intercollegiate athletics. Chief among the division priorities are providing the infrastructure to support the university's development efforts, as well as meet university communication and system-wide advocacy initiatives. To ensure that resources were being used in the most effective way, the division underwent a reorganization that saved the need to hire three new positions, resulting in the savings of $210,000 annually.
- AVP for University Development – A decision was made to move forward on this search as he/she will provide direct leadership support for all development officers and select advancement professionals. This is necessary based on the retirement of Ron Simons.
- Special Event Support for Development Officers - To support the development officer's special event needs, a current position within the division was redeployed to provide full-time event support for all of the colleges' philanthropy efforts.
- Centralized Gift Processing - To ensure that all university gifts are being addressed accordingly, UAD is undergoing a revision to its past practice by centralizing all gift processing. The Director of the Annual Fund will lead both gifts processing specialists towards a new system that will relieve the development directors of this responsibility.
- Data Accountability - To ensure that all university donor and alumni data is absolutely secure as well as creating better data synergy with all prospect research modules, a new structure was initiated to link these two units under single leadership, providing the development team with simpler and more accurate access to prospective donor information.
- Three-Year Advancement Plan Approval – The President approved the three-year plan for the Chancellor's Office (see attached) that address three primary goals for the division. They include:
- Campaign readiness and Major Gift Opportunities
- Advocacy Initiatives
- Division Reorganization and Redeployment
In support of those goals, the division recently has:
- Transferred the governmental affairs office from the President's Office to UA
- Redeployed staff position to support development directors' special events/programming
- Created new option for collateral print materials to support schools
- Restructured gift processing to ensure a more seamless receipt of gifts
- Completed search for new DOD for Engineering
- Begun a search for a new DOD for Athletics
Pending Priorities
- Equitable clerical support for development directors.
- Compensation equity
- Greater support for Alumni Affairs
- Dedicated resources to support Chancellor's advocacy initiatives.
- Following campus prioritization/recovery recommendations, complete a master plan that will address how the university will fund and implement a comprehensive capital campaign.
Next Steps
To date, University Advancement's P/R process has been very effective in identifying division needs, but maybe problematic when comparing our responsibilities with other divisions. After consultation with the leadership of the Academic Affairs Prioritization and Recovery Planning Committee (AAPRPC), it was obvious that University Advancement should retool and use the Academic Affairs model in reviewing our operations. One of UAD's primary responsibilities is to support the academic mission of the university by identifying and acquiring external resources. In many ways, we are "joined at the hip" with colleges. Using the Academic Affairs process will ensure that we are given equitable weight and a level playing field in comparison with other non-academic programs at Cal Poly Pomona.
The foundation of the Academic Affairs model is that it is program-based, not department-based. Programs are evaluated on a number of criteria, many still being defined for non-academic programs.
The first step for University Advancement is defining and identifying our programs. To help with this part of the process, we will use definitions derived from Robert Dickeson's book,Prioritizing Academic Programs and Services.
PROGRAM – An operational definition of a program is any activity or collection of activities that consumes resources (dollars, people, space, equipment, and time).
PROGRAM versus DEPARTMENT – It is important to distinguish between a program and the department that offers it. Programs are usually narrower in nature. A department is an administrative unit and manages several programs. Within University Advancement, it can be complex. (For example, Alumni Affairs is a department that administrates a number of programs, possibly including Professor for the Day, administrative support of the Alumni Association, Distinguished Alumni, etc. University Advancement does have a few programs that stand alone. Two examples would be Gifts Processing and Prospect Research.)
FIRST STEP: Defining our programs.
Office of Alumni Affairs
- Alumni Professor for a Day
- Distinguished Alumni
- Bronco Mentoring
- Student Ambassador
- Alumni Association (Membership, Golf Tournament, Alumni Scholars Program, On-line Community)
Office of Public Affairs
- Clip management & archiving
- Media communications management
- Experts & speakers management
- Crisis & issues management
- Internal communications management (PC, PolyUpdates, master calendar, campus e-mails, photography)
- External & donor communications management (Poly Trends, Panorama,
electronic marquee, annual report/honor roll, division web)
- Brand management & visual identity (graphic standards, university
- Community relations (external as well as campus)
Office of Governmental Affairs
- Resource acquisition
- Policy influence
Annual Fund
- Student assistant training and management
- Direct mail solicitation
- Phonathon solicitation
- E-solicitation
Gift Processing
- Data entry and management
- Donor honor roll
- Gift processing and record management
Information Systems
- Development Data and Programming
- Prospect Research
- IT Maintenance and Support
University Events
- Licensing/Filming of Facilities
- Development Events
- Special Projects
Support Programs
Advisory Board Support
Alumni and Student Programs
Alumni Association
Alumni Outreach and Recognition
Annual Fund
CLASS Development
College of Agriculture Development
College of Business Administration Development
College of Engineering Development
College of Environmental Design Development
College of Science Development
Collins School Development
Corporate and Foundation Relations
CSU Advocacy Initiatives
Development Data and Programming
Development Events
Division of Student Affairs Development
Donor Relations and Stewardship
External Communications and Relations
Gift processing and record management
Governmental Relations - Policy Influence
Governmental Relations - Resource Acquisition
Intercollegiate Athletics Development
Internal Communications and Relations
IT Maintenance and Support
Licensing of Facilities for Events and Filming
Media Communications Management
Planned Giving
Prospect Research
Special Events and Projects
Visual Identity and Branding
VPUA Administrative Operations
Web Application/Programming/Maintenance


text:
Santa Clara Vanguard drum line warming up in parking lot at Mile High Stadium in Denver Co, August 7th 2004 ...
For booking, sponsorship This my contact +2347035146604
Why must I be surrounded by fools?
Pitched up whistling and squeaky tape manipulation with voice recorded on 1/4" tape and physically looped
rhythmic amp and filter envelopes play against each other with fm modulation. bubbly edgy synth, no effects.
break made from random sounds like a drum and bass stomp.
part of my set in dubrovnik.
part of my set in dubrovnik. 2 bar 3/4 loop
Ableton live drum rack loop 123bpm
Short Source of uncertainty driven patch .
circuit bent Casio CTK-550 loop wav sample 5
loops from a track im making.
I Cant Think too MANY THOUGHTS,AHHHHHHHHHHHHHHHH


text:
- Infiniti Pro by Conair® Titanium Ceramic 1-Inch Flat Iron
Brand: Infiniti Pro by Conair
- Categories: More > Beauty & Spa > Hair Dryers & Styling Tools
Achieve professional results with the Infinity Pro Titanium Ceramic Flat Iron by Conair. Infrared energy and titanium ceramic technology combine for less static and frizz, resulting in beautiful, silky hair that looks like you just stepped out of a salon.
- Nexxus Flat Iron
Brand: Nexxus
- Categories: More > Beauty & Spa > Hair Dryers & Styling Tools
Look like you just stepped out of a salon with the Nexxus Flat Iron. An LCD digital temperature setting and touch screen allow you to flip, curl, and straighten your hair with easier control. The 1" floating plates offer great styling versatility.


text:
That's why we've made better heart and brain health our mission. But we're just beginning. Your generous donation will help us create a world free of heart disease and stroke - making a healthier, longer life possible for everyone.
Hear from others as they share their powerful, personal and inspirational Life is Why stories. Why not make your own Life is Why video?
Use the hashtag #lifeiswhy to share your 'why.' Your motivation for living a healthier, longer life could be seen here.
Personalize and buy your Life is Why gear and a portion of the proceeds will benefit the research, programs and life saving initiatives of the American Heart Association and the American Stroke Association. It's a cool way to express who or what motivates you to live healthier.
We invite you to share your "Why" with family, friends, loved ones, and co-workers. Personalize this Life Is Why e-Card to encourage and inspire others to live healthy.


text: I am really into the Idea of Bounded accuracy in 5th editions, and am wundering if anybody has found a good way to house rule it into 4th edition...at least as far as skill checks go. and if nobody has yet maybe they can give me some advice on how I may go about it anyway. =) thanks


text:
Why do readers love to read about rogues and unscrupulous heroes in fiction? Is there something about an unprincipled cad that intrigues us? I say yes, but only if a rebellious heroine tames him through her undeniable beauty, wit and charm. If she comes with her own warts and flaws, all the better. Characters should not be the epitome of perfection. Moreover, this reader is not buying main characters who are faultless and absolute. Everyone wavers between good choices and bad, between darkness and light. We all have a perceived devil with horns that resides in our psyche, tempts us day in and day out to turn away from what we know in our hearts is the "right" path to take. Circumstances sway us, rationalization that one faux pas does not make us corrupt or godless.
In Sojourn With a Stranger, our hero, Derek Stafford has one goal in life--to produce and heir so he can inherit land and title. He's cold and manipulative when the story begins, and not likeable to the reader.
Raine Brinsley, on the other hand, is stalwart, principled and innocent. But circumstances have changed her life and the tenets she's always lived by. (Ah-ha! That little man with horns tempts her to turn from those tenets). Penniless and alone now that her parents drowned at sea, she has to survive, right, and there's only one person in her life that matters now, her ailing, loveable grandfather in Maine.
Derek finds her lovely, possessed of the physical traits he'd like to see in his child. His child. So he makes her an offer--bear my child and I'll give you an enormous sum of money. She wavers and thinks about what she could do with all that money. Return to Maine, seek proper medical attention for her grandfather . . . and wipe every memory of Derek from her mind.
Life seldom turns out the way we'd like, and choices are not always for the greater good. Why? Because the good we see in others changes us, makes us question the choices we've made in our own lives. And because we are human--we fall in love, allow our hearts to overrule logic and the best-laid plans.
Here's an excerpt from Sojourn With a Stranger, a romantic suspense with voodoo, ghosts, mystery and murder. And, oh, yes, darkness and light, good versus evil, and most of all redemption.
Setup: Derek's brother Lyman, visits a voodoo priestess and demands she read tea leaves for clues of his future. He must know what obstructs his path in producing an heir before his brother does. The results of the reading appear as a rhyme or riddle he must solve.
The room smelled of incense and of various pungent herbs boiling in a cauldron over the hearth. Clad in a black silk shift, cut into a deep V that called attention to her plump breasts, the effect against her pale skin summoned Lyman's carnal appetite. Tall and slender, Zaira's slightness belied an inner strength of self-reliance and savoir-faire. Her long hair was the color of a raven's wing, her features small and well defined. An exotic aura defined her. Lyman imagined every man who had ever looked at her wanted her, including him.
"Get to the point, Zaira."
Her haughty air had always incited him. "I thought perhaps you threw the riddle away. I warn you, Lyman, the hour is upon you and the riddle must be solved."
"What on earth are you babbling about?"
She shuttered her eyes, reminding him of a cat. "Messages in the tea leaves, the symbols revealed during the readings."
"Tea leaves, indeed." He clenched his teeth. "Very well, appease me. What did the heathen ritual reveal?"
A smirk curled her erotic mouth.
"How does it work, Zaira? Educate me."
"Reading the leaves is a form of foretelling. My grandmother taught me to interpret the symbols and patterns."
"Ah, yes the wild gypsy who bore your insane mother out of wedlock."
"Who are you to look down on my family? What about your brother's wife, Lucinda? A woman fit for Bedlam."
He dropped his voice and drew the words out. "Yes indeed, poor Cinda. There is a difference though. Derek's wife was not of my blood."
"Bastard," she muttered.
With feigned disinterest he asked, "Do you want to spar all day or tell me how you interpret the leaves?"
"The leaves are dropped into a cup of hot water and I drink it." She licked her bottom lip, and his cock twitched.
"How methodically scientific," he said sarcastically.
"A small amount of liquid is left in the bottom of the cup with the leaves."
Lyman slid into a chair and drummed his fingers on the table. "I can barely contain my excitement over the lesson."
"With the cup in my left hand, I swirl the contents around three times clockwise, making sure the leaves are moved toward the rim."
He rolled his eyes. "Very well, continue."
"I place the cup upside down on the saucer and hold it until the fluids drain, then the cup is placed right side up again with the handle facing me."
"Good heavens, are we ever going to get to the reading, the symbols you spoke of?"
"Then I read or interpret the images, the symbols."
"That's it? Really, Zaira," he sighed and held the riddle before her. "You can't expect one to believe their entire future hangs in the balance over symbols in a cup."
A throaty snicker left her lips. "The leaves are never wrong."
"All right, placate me. You did a reading, thus this note."
She nodded.
"What symbols appeared?"
His head jerked up. "Whose?"
"Those initials could belong to any number of people."
"How many people do you know with those initials?" She smiled again. "If you connect them with the symbols, surely they're your brother's."
Lyman rose from the table, walked toward her and stood inches from her face. "I grow weary of these riddles and games."
Demurring to him, she lowered her head. "If you wish me to stop─"
"On the contrary," he said, taking a step back. "We've gone this far, proceed."
"After the initials, a leaf appeared."
"A leaf?" A short laugh left his lips. "And the meaning is?"
"New life."
Lyman crossed his arms over his chest. "What appeared next?"
"The picture of a cup." The tap of his foot against her creaky floorboards echoed in the small cottage.
"I'm waiting, Zaira."
"If a cup appears it means bloodlines."
"This is absurd!" Finding it hard to grasp air in stuffy room, he launched into a brisk pace. Breathe in; breathe out. "What else?"
"I saw a moon surrounded by dots and a fan."
"Dots and a fan." His emotions vacillated between disbelief and terror. He didn't want to know the meaning of the moon with dots and a fan, yet he had to know. "And the meaning?"
"A moon with dots surrounding it symbolizes an agreement for money, the fan . . . ." Her eyeballs rolled in the sockets, left to right. "An indiscretion."
He tugged at his shirt collar. Damnation. Heat suddenly emanated from every corner of the room. Had he entered a Roman bathhouse? "Let me see if I have this straight." He stopped walking long enough to look at her. "My brother Derek has made an agreement for money to engage in an indiscretion that will result in the birth of an heir?"
Zaira nodded.
"Did the voodoo magic speak to the gender of the child?"
"Not at that reading."
Elation flooded him when she shrank beneath his fierce glare. Zaira wasn't easily intimidated, but apparently she took the readings to heart , believed he should too. "I should throttle you!" He advanced on the table. "Who asked you to perform a reading?"
"Two readings. I only meant to help, prepare you for what lies ahead."
"You lie, Zaira. You did it for money, admit it."
"I-I need the money, yes, but I thought you'd want to know."
He choked on the words. "Know what? My future? All right, what does lie in my future?"
"The second reading revealed an hourglass, an oar and numbers."
"Oh, this is too much. I can't believe I'm standing here, listening to a mad voodoo priestess peddle this manure. "The entire theory is utterly ridiculous."
"An hourglass means time is running out. The oar means success after many failures, and the number speaks for itself."
Looming over her, he spat the words. "What numbers appeared?"
"Only one, the number nine, I interpret that to mean nine months."
"You are spawned from the devil!" His hands reached for her throat. "I should kill you here and now and none would be the wiser."
She flew from the chair and backed away from him. "Wait! If you kill me, I won't be able to help you."
Lyman stopped in mid-flight.
Zaira rushed to the cupboard and pulled a piece of paper from a shelf. "Here's my interpretation from the second reading."
He grabbed it from her outstretched hand. "Another riddle?" he asked in low humor.
The hour is upon us, the day is fading fast.
The maiden's made her choices, his seeds will soon be cast.
A male comes to the manor, with eyes of emerald green.
Raise the goblets; wave the banners, the Stafford heir undoes the scheme.
I hope you enjoyed the excerpt! Thanks so much for dropping in.
Kindle: http://amzn.to/I0tYJK
Nook: http://bit.ly/KicQAa
Nook UK: http://bit.ly/SxdyyG
Kobo: http://bit.ly/NrzDKC


text:
Tesla model year changes do not coincide with updates and improvements. Rather, Tesla simply adds features and options as they become technically feasible. Hence the center console data screen shows fields for blind-spot warning, lane-departure warning and all-wheel drive, all desirable, but with none not yet offered on the 2014 car.
For 2013, the Model S now has the ability to receive over the air software updates, providing buyers with new features and services as they become available.
Designed from the start as an EV, the 2015 Tesla Model S is a roomy 5-passenger electric sedan boasting excellent cargo room with advantages in range and performance that its rivals can't touch. Tesla itself is run more like a tech company than an automaker, making for a unique ownership experience that could hint at the future of the auto industry. For now though, the Tesla Model S stands alone, with its long cruising range and network of "Supercharger" charging stations taking some of the guesswork out of recharging. This year, the all-wheel-drive, high-performance P85D Model S debuts. It can blow the doors off virtually any sedan you'd care to mention...and a lot of supercars as well.View Tesla Model S vehicles for sale near you.
By John on Saturday, June 06, 2015
I own this car - My approximate mileage is 18,500overall rating 10 of 10rating details
Pros: "VERY fun to drive. Safe and longest driving range"
Cons: "Seats are a bit spartan."
Likely to recommend this car? (1-10): 10
"I drive over 80 miles every day in ATL bumper to bumper traffic and this car handles everything Atlanta throws at it. The automatic cruise control has saved me from being in several accidents. The supercharger network is just about perfect for me driving to Auburn, around in ATL and Decatur there is plenty of superchargers available. Only wish there was 1 in Augusta. Supercharger charge rate from 50 miles range to 200 is about 45 minutes and it is free. I have the Tesla in-home 80 amp charger installed too. I just hook it up when I get home and when I need to go somewhere the Tesla is charged up ready to go. Power usage for us is about $60 a month driving 80-100 miles a day. WAY cheaper than the $400 a month in gasoline expense! Tesla Needs to have a better console although upgrade is available for $600 which I'll be installing. Tesla needs a better standard stereo and I hear the upgraded stereo is not much better. However the stereo is better than what was in my Volvos. We also have a leaf which we enjoy for short hops but the range is only useful for driving to work and back (barely). Driving it always leaves me wishing to take the Tesla instead so we'll probably be buying a second one soon."
9 people out of 9 found this review helpful
Was this review helpful to you? yes | no | report abuse
By Ruscle on Tuesday, June 23, 2015
I own this car - My approximate mileage is 72,000overall rating 10 of 10rating details
Pros: "50 characters is not enough."
Cons: "Tires last about 25,000 miles or less."
Likely to recommend this car? (1-10): 10
"Do the math... *Gas was $700/mo... now zero. *No engine to maintain. *Electric bill before Model S was $250 and rising. *Solar panels and night charging... *electric bill is now $150. *Price of a 400 mile round trip via Superchargers = $4 [$0.11/kw to refill the battery at home after the journey.] *Time savings not going to gas stations, oil changes, service. *72,000 miles and 30 months later we love our Model S almost as much as the day we got it."
4 people out of 4 found this review helpful
Was this review helpful to you? yes | no | report abuse
By JB on Saturday, May 04, 2013
I own this car - My approximate mileage is 300overall rating 10 of 10rating details
Pros: "Efficiency, touch controls and a joy to drive"
Cons: "Too few cup holders, missing a couple features"
Likely to recommend this car? (1-10): 10
"I've driven lots of nice cars, from BMW's to Porsche's, and my Model S is the best car I've ever owned or driven. To be able to combine the performance of an exceptional sports car with comfort of a luxury car AND the efficiency better than any hybrid is what I find to be most remarkable. Range is the biggest compromise, but my range so far has been close to rated (200+ miles on regular charge with the 85kw battery and limited use of climate control), which is fine for me 95% of the time (will swap with my wife's car for longer trips where I don't want to worry about it). Also, love the touch screen center stack...definitely ahead of its time and it works great. The car has a feel of quality. Yes, it's missing some features (parking sensors, blind spot monitor, etc), but the sum of the car more than makes up for the lack of a couple missing options. I have also had a couple minor hiccups -- defective 3G antenna needed to be replaced and waiting on a fix to the tire pressure warning that keeps popping up, but overall, I love the car. Best advise for anyone doubting the performance or otherwise on the fence is to drive the car."
90 people out of 94 found this review helpful
Was this review helpful to you? yes | no | report abuse


text:
Former Bain students now working on staff
By Michele Dotson
Whoever said "Nothing good can last forever," certainly had never attended Bain Elementary School.
Five current members of the Bain Elementary staff are former students. What has drawn them back to Bain to work as adults is basically the same feelings they got when they were kids.
"The closeness that we have is like an extended family," says Administrative and Financial Secretary Dorothy Flaherty who attended Bain from Kindergarten through sixth grade.
Third grade teacher Jessica Corrigan is proud to be at Bain to continue the traditions.
"The heart of Bain is what makes it so special. There is a feeling of love and warmth amongst the staff, parents, and students. We have strong historical ties to the community and Bain is rooted in a long tradition of providing the best for its students. "
Academic Facilitator Kelli Drye's mother taught at Bain when she was a student there and she remembers spending time after school with her in her classroom "teaching school."
Although Bain has been known since its inception in 1889 as a quality educational institution, holding high expectation of its students and parents, there's always room for some fun, too. […]
CMS bond requests funds for programs, schools in Mint Hill area
A portion of the $290 million Charlotte Mecklenburg Schools is requesting through a bond referendum will directly assist Mint Hill area schools.
If approved, approximately $39.02 million will be directed for a new K-8 Magnet school and updating Career and Technical Education programs.
$30.38 million would fund the construction of a new K-8 partial magnet school, expanding […]
Principal readies Bain for transition
By Michele Dotson – Staff Writer
Finishing touches are being completed at the newly constructed Bain Elementary School in preparation for the start of school in August. The project, which cost more than $15 million and took about 18 months to complete was paid for with 2007 bond money.
The school was first slated only for upgrades, but approval for a new structure came once it was determined to be more cost effective to build from scratch. Construction began in early spring of 2012. nths to complete was paid for with 2007 bond money.
Principal John LeGrand has spent the summer organizing the move from the adjacent building and readying the school for the return of teachers on August 19.
"I have been addressing our instructional focus, of course, but I've been surprised how I've been pulled into dealing with lots of logistics this summer." […]


text:
Local News
Cat Rescue seeking donations for bake sale, craft bazaarNovember 19, 2014
SHERIDAN — Second Chance Sheridan Cat Rescue is seeking donations for a holiday bake sale and craft bazaar, both scheduled for Saturday. All proceeds from these events will go to Second Chance Sheridan Cat Rescue. The organization is accepting any homemade baked goods for the bake sale at Warehouse Market on Saturday from 10 a.m. Read More
Thursday Events Nov. 19, 2014
Events for Thursday, November 20, 2014 • 5-7 p.m., SAGE holiday art show and sale reception, Sheridan County Fulmer Public Library, 335 W. Alger St. • 7:30 p.m., Danish vocal group "Basix," WYO Theater, 42 N. Main St., $20 for adults, $18 for seniors and students
Local Briefs Nov. 19, 2014
Civic Theatre Guild to perform 'The Prisoner of Second Avenue' SHERIDAN — The Sheridan Civic Theatre Guild will perform "The Prisoner of Second Avenue" this weekend at the Carriage House Theater. Dimitra Dugal will direct the dramatic play by Neil Simon in which a well-paid executive of a high-end Manhattan firm gets the ax. While Read More
How does a city design paradise?November 18, 2014
City considers conceptual plans for large community park SHERIDAN — Conceptual plans for a proposed park on West Fifth Street have been released and were presented to City Council during a work session Monday. The park is proposed to include approximately 10 acres of multi-purpose sporting fields and more than 20 acres of open space Read More
Sheridan Swim Team bazaar to feature more than 100 tables of items
SHERIDAN — More than 100 tables of arts, crafts and homemade goods will be featured at Saturday's Sheridan Swim Team bazaar. The event will run from 9 a.m. to 4 p.m. at the Sheridan County Fairgrounds exhibit hall and pavillion. Lunch and a bake sale will also be available on-site. For additional information contact Shirley Read More
Sunrise Fall Bazaar open Saturday
SHERIDAN — The Sunrise Fall Bazaar will run from 8 a.m. to 2 p.m. Saturday at the Sunrise Assembly of God Church. The event will feature baked goods, a quilt raffle, food and more. A kids store with help for gift wrapping will also be available. For additional information, contact Crystal O'Brien at 674-6743. The Read More
Dayton artists open doors to guests Saturday
DAYTON — Eight artists in five locations will open their studios to guests on Saturday from 10 a.m. to 4 p.m. for the Dayton Art Loop studio tour. Artists participating in the event include Sonja Caywood, Iris Sorensen, Alice Fuller, Barb Sellar, Nikki Yeigh, Joan Feagins, Melanie Anseth and Haleigh DeRoacher. Caywood will display in her Read More
Big Horn Woman's Club to meet Friday
BIG HORN — The Big Horn Woman's Club will meet at 1:30 p.m. Friday at the clubhouse in Big Horn. Fachon Wilson will present a history of the Sheridan sculpture project. The meeting is free and open to all women. For additional information, contact Kathy Hosford at 674-7656. The Big Horn Woman's Club clubhouse is located Read More
SHS announces first quarter Principal's Honor Roll
SHERIDAN — Sheridan High School officials released the school's first-quarter honor rolls last week. To be eligible for the Principal's Honor Roll, students must be enrolled in fives classes at Sheridan High School and earn all As. Ninth grade Hunter Andersen, Christopher Brown, Kirby Coe-Kirkham, Olivia Conrad, Bailey Coon, William Craft, Kyle Custis, Lennon Dregoiw, Read More
Wednesday Events Nov. 18, 2014
Events for Wednesday, November 19, 2014 • 11 a.m. to noon, Affordable Care Act marketplace presentation, conference rooms A and B, Sheridan Memorial Hospital, 1401 W. Fifth St. • 10 a.m. to 3 p.m., Sheridan College AgriPark Open House, Chris LeDoux Way
Local Briefs Nov. 18, 2014
SC to host AgriPark open house SHERIDAN — The Sheridan College AgriPark will hold an open house on Wednesday. The open house will run from 10 a.m. to 3 p.m., with a special recognition ceremony from 12:15-1 p.m. It is free and open to the public. The open house will include guided tours by the Read More
Students: We want our laptops back
BIG HORN — "They want their laptops back" was the message that trustee Carol Garber brought to the Sheridan County School District 1 board of trustees after meeting with the Big Horn High School and Middle School students. In the first round of student meetings, trustees divided up schools in the district to visit with Read More
Three cities team up to promote NE Wyo. to national businesses
SHERIDAN — The cities of Sheridan, Buffalo and Gillette have teamed up to pursue a Wyoming Business Council planning grant that will enable the three cities to launch a national marketing initiative to promote northeastern Wyoming as a desirable location for business relocation and expansion. Members of City Council voted Monday to approve a resolution Read More
City passes resolution outlining liquor license application policy
BIG HORN — With the latest in a long line of steps toward the construction of the new Tongue River Elementary School completed, the outlook for the project is positive. A mandatory pre-bid meeting was held, inviting construction companies to tour the site, ask questions of the architect and learn more about the scope of Read More
SCSD1 officials confident new TRE project will move forward
BIG HORN — With the latest in a long line of steps toward the construction of the new Tongue River Elementary School completed, the outlook for the project is positive. A mandatory pre-bid meeting was held, inviting construction companies to tour the site, ask questions of the architect and learn more about the scope of Read More
City ceases efforts in development of entrepreneurial support facility
SHERIDAN — The city of Sheridan and Downtown Sheridan Association Monday announced intentions to cease pursuing an entrepreneurial support program facility in downtown Sheridan. In 2013, the DSA and the city partnered to explore the possibility of rehabilitating a building in the historic downtown to serve as a location for providing entrepreneurial support for downtown Read More
City waiting on approval for hydropower loan application
SHERIDAN — If a loan application is approved, the city of Sheridan will be able to produce hydropower on one of its raw water mains in the future and sell the power back to Montana-Dakota Utilities. City Council on Monday approved a resolution authorizing submittal of a loan application to the State Loan and Investment Read More
Historic business in Sheridan for sale: Sheridan Iron Works owners prepare to close up shopNovember 15, 2014
SHERIDAN — The Sheridan Iron Works sign is a comforting sight in Sheridan. The first lighted sign in Sheridan, it has been glowing on A Street since 1910. The future of that sight and that sign, though, is uncertain. "I'll tell you that I'm going to be really picky about who buys this place," Sue Read More
SCSD3 Board of Trustees approves wage increase for subs
CLEARMONT — It was a cold night for a drive in the country Wednesday as the Sheridan County School District 3 Board of Trustees gathered for its monthly meeting. In an attempt to escape the bitter cold and snowy roads before it was too dark, the meeting — regularly scheduled for 7 p.m. — was Read More
Fostering a new kind of family : Vicki Nyback opens her door to veterans in need
SHERIDAN — Commercial door hinges are supposed to last a long time — under normal wear and tear. The wear and tear on Vicki Nyback's home is definitely not normal, and that's OK with her. "We've lived in this house over 30 years, and my husband uses commercial hinges on our front door," Nyback said. "He said Read More
Local Briefs Nov. 15, 2014
Polka Club to meet Sunday SHERIDAN — The Big Horn Mountain Polka Club will host a dance at the Elks Lodge Sunday from 1-5 p.m. Music will be performed by the Wyoming Drifters and dancing will include polka, waltz and two-step. The event is free and open to the public. The Elks Lodge is located Read More
Sunday & Monday Events Nov. 15, 2014
Events for Sunday, November 16 and Monday, November 17, 2014 Sunday • 1 p.m., Let's Dance, Sheridan Elks Lodge, 45 W. Brundage St., $5 per person • 2 p.m., "The Prisoner of Second Avenue," Carriage House Theater, 419 Delphi Ave., $15 for adults, $12 for students, seniors and active military • 1-5 p.m., Big Horn Read More
Agendas Nov. 15, 2014
Sheridan City Council 6-6:45 p.m. work session Council Chambers Sheridan City Hall 55 Grinnell St • Park and Recreation Master Plan Update and West Fifth Street Park Plan Regular Council meeting 7 p.m. • Call to Order • Pledge of Allegiance to the flag • Invocation • Roll Call of members • Approval of Consent Read More
FIB extending coat drive to Dec. 1November 14, 2014
SHERIDAN — The Sugarland Branch of First Interstate Bank has extended its winter coat drive through Dec. 1. Organizers have asked community members to bring gently used, clean coats to the office for distribution to clients of Volunteers of America Northern Rockies. For more information, contact David Hubert at 672-1501. The Sugarland Branch is located Read More
WGFD Conservation Stamp Art Contest to feature swift fox
SHERIDAN — The Wyoming Game and Fish Department will begin accepting entries for the 2016 Conservation Stamp contest Jan. 1. Artists are asked to submit their designs for the 32nd annual Conservation Stamp Art Competition by March 1. The selection of the competition winner will take place April 10. The judging will be held in Read More


text:
CBCBMedical Dispensaries / Berkeley (235,664 hits)
We are a patient centered cooperative bringing patients access to a natural perspective of wellness through access to high quality medicine, educational seminars, support groups, and a variety of services. Services are included in membership with no additional fee. Please call for more information & Follow us on Twitter! Follow @CBCBerkeley
Great Bud, Expensive Edibles
First of all let me start by saying out of the four staff I encountered only one of them seemed friendly, and he (Coleman) is the only reason I'll probably come back just because he was so friendly and helpful and I'm hoping that other staff I didn't meet are that way. The front desk person seem really uninterested and not excited or friendly like you should be to new patients (First impressions are important! They set the tone!) the security guard at the main entrance didn't even say a word to us. No "Hello" "How's it going" nothing. Their papers that are required for new patients ask for a bit more info than other clubs. They want your Doctor's name and phone number, what you can offer to the club and what activities you can do with the club (I thought this was a tad strange). The bud they have there looks and smells nice, the bud I received (I bought a 16th of 3 X Crazy) smelled great, but the container it was in also produced it's own smell because it seemed like a cheap thin plastic. Their edibles are overpriced to the extreme. A 60MG edible was $15. I could have bought an edible with higher dosage for $8 (150MG) and their $5 edibles are only 25MG.. their prices don't make much sense to me at all on that. One thing I was really impressed with was the glass they had for sale. Nice thick glass with an extremely affordable price. Nice big bubblers were $40, and small bubblers were $15. I purchased a bong on the smaller size for $30 and it came with a really basic downstem and bowl. I think they should offer upgrades on the downstem and bowl as both are just the ordinary bowls and downstems you would find in a smokeshop. Overall CBCB is an OK establishment. There is definitely room for improvement.
Room for improvement
CBCB has some issues, in my opinion. The biggest ones are: tax not being included in the price, they cut down the buds so they're all small, and the staff is not very knowledgeable. I really don't like that tax isn't included in the price. Anytime I buy something, I feel like I'm overpaying, because I buy top shelf and it's almost an extra $5 a lot of times. Almost all of the other clubs include tax in the listed price, which tells me it's possible and they choose not to. The last 4 times I've gotten flowers from CBCB, the display looked nice (big, frosty buds), but when I got home and opened the container, it looked like shake/popcorn buds. I'm sure they're doing this to get the weight EXACT so they can make more money. I've learned that if I have a question about a product I should just Google it, because I never get good answers from the staff. I had a question about a tincture a few weeks ago, and the answer I got was, "I have no idea." This has happened many times before.
In my opinion, the only reason to go to CBCB is if they have a strain of flowers you really want. Unless you are prepared to drop a lot of money on concentrates, stay clear. The $20-30 concentrates are all dark brown and don't look appealing at all. Sometimes you'll find a nice gram of wax on the lower end, but unless you are going to spend $45, go somewhere else for your concentrates.
My go to for nectar
I like the points system no.1. I always get a free roll with a purchase over 60.00. I have had their Karma wreck that they made in house--was pretty delightful. Cherry AK 47 was also on point. I just tried the LSD and it smells lovely for sure! couldn't touch the bud before i bought it and noticed it was super dry--but goes far in the grinder. The taste is perfumey--which is the only down fall. Maybe it wasn't cured right? I probably wouldn't buy LSD again.. but they have amazing selections! I like that the collective feels community based..many events take place and has a cozy feel to it.
Chill spot
Friendly staff and even the budget strains are pretty good. Also close to Bart which is awesome.
My go to spot when I'm in the area
Always smooth experience when I'm visiting. Chill atmosphere.
Customer Svc LACKING!
This review is not an indicator of all the svc person there however the AA female in the black dress on 6/13/015 was the worst! This was my first time at a dispensary and I'm not a long time user so I was looking forward to getting some education on product. As I stood in line I observed the customers in front of me getting full explanations of differing products-encouraged that I would receive the same I was then called by another sales person. OMG! I asked about getting a mild indica and asked for suggestions. She brought ONE variety Full Sun OG, She never asked me what effect I was trying to get-I explained that this was my first time purchasing and needed more information to that she replied "well its our lowest grade so its not usually that strong". That was the extent of her imparting any information. I purchase 1/8th-she gave me a first timers joint and a cookie.....both will be giveaways since I have no idea what strain is in either. After I left I realize I should have asked to have another person assist me. It was a very disappointing visit-I will return but I will NOT allow that individual to assist me and hopefully leave more educated.
Y'all need to include tax in your prices
2 peoplethought this review was helpful!
All the other dispensaries do. End of discussion.
This is hands down my favorite dispensary. The staff is friendly & helpful & you can get pretty much anything you want. Whether you're looking for a decently priced half or a top shelf eighth, they're able to help you. Only time I go to another dispensary is when I'm on vacation. CBCB is where it's at!
My Berkeley Favorite
This is always my first option. The best is when you can park on the property but finding a spot in the neighborhood is not a hassle. Security is great and staff is awesome. Bud is always the best quality so sometimes you have to pay up for top shelf flowers. Wide variety of concentrates and edibles are always available. Different services are held daily and I'm happy to use my student discount here.
How do I love you let me count the ways
1 personthought this review was helpful!
I came in mad and left happy as hell. The bud lookd like some fire fire flame. The guy at the front was cool hella paper work though one minus. I must say the highlight of my trip was my budtender. With her pretty self. I think her name was tyler or tayler I couldn't really hear cause some guys were playing live behind us ( :-/) She was legit hella smart got me set up nice with doobies and edibles. this place is endless I could spent days there. Everyone just looked hella nice and chill ( hella stoned) but I loved it! And they have 100$ OZs!! In other words come here.
Confirm your email
Please confirm your email before writing a review.Resend email confirmation?


text:
About the artist
The band has shared stages with bands like Hockey, Magic Man, X Ambassadors, Donald Cumming, Har Mar Superstar, Gold Fields, JD Samson, Said The Whale and Public Access TV. On February 19 and 23 of 2012, the band opened up for Guns N' Roses in Chicago and Maryland. Born Cages' latest music video for Rolling Down The Hill won mtvU's The Freshmen competition on November 21, 2014. The single reached No. 7 on the Alternative Specialty Radio chart.
Description provided by Wikipedia under Creative Commons Attribution CC-BY-SA 4.0


text:
Book your room at Hotel du Grand Paradis
Get an overview of this hotelRomantic ski retreat with a full-service spa
Located near the slopes, this Cogne hotel is in the mountains, 2.5 mi (4.1 km) from Lillaz Waterfalls, and within 20 mi (32 km) of Introd Animal Park and Cote de Gargantua Natural Reserve. Sarre Royal Castle and Sarriod de la Tour are also within 16 mi (25 km).
Hotel Features
Skiing and snowboarding are not the only attractions at Hotel du Grand Paradis, where you can enjoy a meal at the restaurant, take a dip in a spa tub, and relax in a full-service spa.
Room Amenities
All 27 rooms feature thoughtful touches like bathrobes and slippers, along with free WiFi and digital TV. Guests will also find room service, free newspapers, and hair dryers.Information missing or incorrect? Tell us!
Hotel Amenities
Hotel Amenities
In addition to a full-service spa, Hotel du Grand Paradis features a spa tub and a sauna. The hotel offers a restaurant. A complimentary breakfast is offered each morning. High-speed wireless Internet access is complimentary.
This ski hotel also offers a fitness center, a steam room, and a library. Limited complimentary onsite parking is available on a first-come, first-served basis.
- Steam room
- Sauna
- Fitness facilities
- Spa tub
- Babysitting or childcare (surcharge)
- Total number of rooms - 27
- Number of floors - 3
- Coffee/tea in lobby
- Free newspapers in lobby
- Free breakfast
- Dry cleaning/laundry service
- Elevator/lift
- Express check-in
- Express check-out
- Full-service spa
- Turkish bath/Hammam
- Free WiFi
- Laundry facilities
- Library
- Parking (limited spaces)
- Garden
- Luggage storage
- Restaurant
- Year Built 1899
- Train station pickup (surcharge)
- Free self parking
- Ski shuttle
- Ski storage
Family Friendly Amenities
- Free Wi-Fi
- Laundry facilities
- Babysitting or childcare (surcharge)
Available in all rooms: Free WiFi
Available in some public areas: Free WiFi
Free self parking, Parking (limited spaces)
Room Amenities
- Hypo-allergenic bedding available
- Free newspaper
- Access via exterior corridors
- Phone
- Daily housekeeping
- Private bathroom
- Bathrobes
- Free toiletries
- Hair dryer
- Bidet
- Slippers
- Shower only
- In-room safe
- Desk
- Digital TV service
- Free WiFi
- Room service (limited hours)
Where to Eat
Guests are offered a complimentary buffet breakfast each morning.
Hotel du Grand Paradis has a restaurant on site.
Room service (during limited hours) is available.
Nearby Things to Do
Recreational amenities at the hotel include a spa tub, a sauna, a steam room, and a fitness center.
The recreational activities listed below are available either on site or nearby; fees may apply.
- Downhill skiing nearby
- Bicycle rentals nearby
- Mountain biking nearby
- Ski lessons available nearby
- Ski equipment rental shops nearby
Spa Services
Guests can indulge in a pampering treatment at the hotel's full-service spa, La Baita. Services include massages, facials, body scrubs, and body treatments. The spa is equipped with a sauna, a spa tub, and Turkish bath/hammam.
Children under 12 years old are not allowed in the spa without adult supervision.
- Body scrubs
- Sauna in spa
- Spa tub in spa
- Massages
- Body treatments
- Facial treatments
To make requests for specific accessibility needs, complete the "Special or Accessibility Requests" section of "Trip Preferences" when you book your hotel.
- Accessible bathroom
- Accessible path of travel
- In-room accessibility
Hotel Policies
Check-in time starts at 2 PM
Payment types
Children and extra beds
- Children are welcome.
Pets allowed
You need to know
Extra-person charges may apply and vary depending on hotel policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
- Tourism fee: EUR 0.80 per person, per night
- Self parking fee: EUR 10 per night
- Pet deposit: EUR 10 per night
We should mention
Cash transactions at this property cannot exceed EUR 999.99, due to national regulations. For further details, please contact the property using information in the booking confirmation. This property offers transfers from the train station (surcharges may apply). Guests must contact the property with arrival details before travel, using the contact information on the booking confirmation. Reservations are required for massage services and spa treatments. Reservations can be made by contacting the hotel prior to arrival, using the contact information on the booking confirmation.


text:
By clicking the "Submit" button, you agree to Avvo's Terms of Use. Please see our Community Guidelines and Privacy Policy for information on posting to Avvo and how we collect, use, and share information you provide to us.
400 Marshall RdPearl Harbor, HI
Licensed for 17 years
Go back to Peter's profile.


text:
Premier Program JOIN FREE!
Premier Members Enjoy:
Exclusive pricing and volume discounts
3% BACK on every purchase
Fast & FREE shipping on thousands of items
Save time with your account manager
Over 7 million products to choose from
Quick & easy PO terms for qualifying accounts
Buying for a business, school, or organization?Save even more with our FREE Premier Program for business professionals. CONTINUE
Joola USA Rollomat Indoor Table Tennis Table
Joola USA Rollomat Indoor Table Tennis Table
Weights & Dimensions
|Product Type: Indoor table
|Age Group: 11 to 12 Years; 13+ Years
|Table Top Material: Wood surface
|Accessories Included: Yes
|Legs Included: Yes
|Single Player: Yes
|Foldable: Yes
|Portable: Yes
|Tournament Grade: Yes
|Country of Manufacture: Germany
About the ManufacturerThe brand JOOLA has it's origin in the 1950's when it was popular to combine the names of companies and cities. The sports department of the department store "Jooss" in Landau, Germany was involved in the production of the first table tennis tables, in 1952. The credit of the establishment of this brand rests with Karl Frey.
Joola USA Rollomat Indoor Table Tennis Table
Still have questions about this product? We're here to help
Customer Reviews
Tell Us What You Think!
Tell us what you think about this product. It will help us provide you with better products in the future and help your fellow shoppers make better purchases.Write the first review
OUR SKU # JLA1088Rollomat Indoor Table Tennis Table is also sometimes listed under the following manufacturer numbers: 11410, Rollomat - Green