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Publications (24)208.61 Total impact
- SourceAvailable from: sciencedirect.com
Article: Corrigendum to "Engineering polypeptide coatings to augment gene transduction and in vivo stability of adenoviruses" [J. Control. Release 166 (2013) 75–85]Journal of Controlled Release 12/2013; 172(3):1161. DOI:10.1016/j.jconrel.2013.08.035 · 7.26 Impact Factor
Article: Rapamycin Enhances Adenovirus-Mediated Cancer Imaging and Therapy in Pre-Immunized Murine Hosts[Show abstract] [Hide abstract]
ABSTRACT: Tumor-specific adenoviral vectors comprise a fruitful gene-based diagnostic imaging and therapy research area for advanced stage of cancer, including metastatic disease. However, clinical translation of viral vectors has encountered considerable obstacles, largely due to host immune responses against the virus. Here, we explored the utilization of an immunosuppressant, rapamycin, to circumvent the anti-adenovirus immunity in immunocompetent murine prostate cancer models. Rapamycin diminished adenoviral-induced acute immune response by inhibiting NF-κB activation; it also reduced the scale and delayed the onset of inflammatory cytokine secretion. Further, we found that rapamycin abrogated anti-adenovirus antibody production and retarded the function of myeloid cells and lymphocytes that were activated upon viral administration in pre-immunized hosts. Thus, the co-administration of rapamycin prolonged and enhanced adenovirus-delivered transgene expression in vivo, and thereby augmented the imaging capability of adenoviral vectors in both bioluminescent and positron emission tomography modalities. Furthermore, we showed that despite an excellent response of cancer cells to a cytotoxic gene therapeutic vector in vitro, only minimal therapeutic effects were observed in vivo in pre-immunized mice. However, when we combined gene therapy with transient immunosuppression, complete tumor growth arrest was achieved. Overall, transient immunosuppression by rapamycin was able to boost the diagnostic utility and therapeutic potentials of adenoviral vectors.PLoS ONE 09/2013; 8(9):e73650. DOI:10.1371/journal.pone.0073650 · 3.53 Impact Factor
Article: Engineering Polypeptide Coatings to Augment Gene Transduction and in vivo Stability of Adenoviruses.[Show abstract] [Hide abstract]
ABSTRACT: We sought to modify adenoviral (Ad) particles by incorporating the advantageous characteristics of non-viral gene delivery vehicles to complement the viral vectors. α-Amino acid-N-carboxyanhydride chemistry was used to synthesize homopolypeptides and diblock copolypeptides that possess well-defined secondary structures. Using cryo-electron and fluorescence microscopy, we showed that these polypeptides can coat the surfaces of Ad particles in a non-covalent manner to modify their transduction properties. The coated Ad particles were found to bind to and be internalized by cells. In contrast to reports using covalently PEGylated Ad particles, we found that our physically coated Ad hybrid complexes facilitate gene transfer both in vitro and in vivo. We showed that our polypeptide coating was able to shield the Ad particles from the neutralizing effect of antibodies and mitigate the binding of blood coagulation factor (Factor X) in vitro. The coating also reduced the antigenicity of Ad in immunocompetent mice. The biodistribution of the systemically administered hybrid complexes mirrored the behavior of both viral and non-viral vectors, exhibiting liver tropism as well as enhanced lung transduction. These data demonstrated that our non-covalent modification was able to alter Ad's interactions with cells and organs with retention of transduction efficiency. Advantages such as facile coating of the Ad vector, design flexibility and ease of attaching ligands to the polypeptides make this system potentially useful as a platform for adding functionalities to Ad to target cancer metastasis.Journal of Controlled Release 12/2012; 166(1). DOI:10.1016/j.jconrel.2012.10.023 · 7.26 Impact Factor
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ABSTRACT: Better intraprostatic cancer imaging techniques are needed to guide clinicians in prostate cancer treatment decisions. Because many genes are specifically overexpressed in cancer cells, one strategy to improve prostate cancer detection is to image intraprostatic cancer-specific transcriptional activity. Because of the obstacles of weak cancer- or tissue-specific promoter activity and bladder clearance of many PET tracers, intraprostatic PET of gene transcriptional activity has not been previously reported. The two-step transcriptional amplification (TSTA) system that amplifies the prostate-specific antigen promoter activity was used for PET imaging of the reporter gene herpes simplex virus type-1 sr39 thymidine kinase (HSV1-sr39tk). The TSTA-sr39tk system was injected directly into prostates or prostatic tumors as a replication-incompetent adenovirus (AdTSTA-sr39tk) and imaged using PET. AdTSTA-sr39tk was able to image prostate-specific antigen promoter transcriptional activity by 9-(4-(18)F-fluoro-3-[hydroxymethyl]butyl)guanine PET, in both mouse and canine prostates in vivo. Ex vivo small-animal PET images, scintigraphic counts, and sr39tk expression analysis confirmed the specificity of the observed signal. Here, by combining the TSTA-amplified signal with a protocol for tracer administration, we show that in vivo PET detection of transcriptional activity is possible in both mouse and immunocompetent canine prostates. These results suggest that imaging applications using transcription-based tumor-specific promoters should be pursued to better visualize cancer foci that escape detection by conventional biopsies.Journal of Nuclear Medicine 05/2011; 52(5):784-91. DOI:10.2967/jnumed.110.084582 · 5.56 Impact Factor
Article: 2152 A DUAL ADENOVIRAL AMPLIFICATION SYSTEM BASED ON PSA-PROMOTER TO INCREASE SENSITIVITY OF DETECTION OF PROSTATE CANCER CELLS IN VIVO BY NON INVASIVE IMAGINGThe Journal of Urology 04/2010; 183(4). DOI:10.1016/j.juro.2010.02.2254 · 3.75 Impact Factor
Dataset: Targeting distinct tumor-infiltrating myeloid cells by inhibiting CSF-1 receptor: combating tumor evasion of antiangiogenic therapy[Show abstract] [Hide abstract]
ABSTRACT: Tumor-infiltrating myeloid cells (TIMs) sup-port tumor growth by promoting angiogen-esis and suppressing antitumor immune responses. CSF-1 receptor (CSF1R) signal-ing is important for the recruitment of CD11b F4/80 tumor-associated macro-phages (TAMs) and contributes to myeloid cell-mediated angiogenesis. However, the impact of the CSF1R signaling pathway on other TIM subsets, including CD11b Gr-1 myeloid-derived suppressor cells (MDSCs), is unknown. Tumor-infiltrating MDSCs have also been shown to contribute to tumor angiogenesis and have recently been impli-cated in tumor resistance to antiangiogenic therapy, yet their precise involvement in these processes is not well understood. Here, we use the selective pharmacologic inhibitor of CSF1R signaling, GW2580, to demonstrate that CSF-1 regulates the tumor recruitment of CD11b Gr-1 lo Ly6C hi mono-nuclear MDSCs. Targeting these TIM subsets inhibits tumor angiogenesis associated with reduced expression of proangiogenic and immunosuppressive genes. Combination therapy using GW2580 with an anti– VEGFR-2 antibody synergistically sup-presses tumor growth and severely im-pairs tumor angiogenesis along with reverting at least one TIM-mediated anti-angiogenic compensatory mechanism in-volving MMP-9. These data highlight the importance of CSF1R signaling in the recruitment and function of distinct TIM subsets, including MDSCs, and validate the benefits of targeting CSF1R signaling in combination with antiangiogenic drugs for the treatment of solid cancers. (Blood.
Article: Targeting distinct tumor-infiltrating myeloid cells by inhibiting CSF-1 receptor: combating tumor evasion of antiangiogenic therapy[Show abstract] [Hide abstract]
ABSTRACT: Tumor-infiltrating myeloid cells (TIMs) support tumor growth by promoting angiogenesis and suppressing antitumor immune responses. CSF-1 receptor (CSF1R) signaling is important for the recruitment of CD11b(+)F4/80(+) tumor-associated macrophages (TAMs) and contributes to myeloid cell-mediated angiogenesis. However, the impact of the CSF1R signaling pathway on other TIM subsets, including CD11b(+)Gr-1(+) myeloid-derived suppressor cells (MDSCs), is unknown. Tumor-infiltrating MDSCs have also been shown to contribute to tumor angiogenesis and have recently been implicated in tumor resistance to antiangiogenic therapy, yet their precise involvement in these processes is not well understood. Here, we use the selective pharmacologic inhibitor of CSF1R signaling, GW2580, to demonstrate that CSF-1 regulates the tumor recruitment of CD11b(+)Gr-1(lo)Ly6C(hi) mononuclear MDSCs. Targeting these TIM subsets inhibits tumor angiogenesis associated with reduced expression of proangiogenic and immunosuppressive genes. Combination therapy using GW2580 with an anti-VEGFR-2 antibody synergistically suppresses tumor growth and severely impairs tumor angiogenesis along with reverting at least one TIM-mediated antiangiogenic compensatory mechanism involving MMP-9. These data highlight the importance of CSF1R signaling in the recruitment and function of distinct TIM subsets, including MDSCs, and validate the benefits of targeting CSF1R signaling in combination with antiangiogenic drugs for the treatment of solid cancers.Blood 12/2009; 115(7):1461-71. DOI:10.1182/blood-2009-08-237412 · 9.78 Impact Factor
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ABSTRACT: Imaging in medicine has been classically based on the anatomical description of organs. In the past 15 years, new imaging techniques based on gene expression that characterize a pathological process have been developed. Molecular imaging is the use of such molecules to image cell-specific characteristics. Here, we review recent advances in molecular imaging, taking as our prime example lymph node (LN) metastasis in prostate cancer. We describe the new techniques and compare their accuracy in detecting LN metastasis in prostate cancer. We also present new molecular strategies for improving tumor detection using adenoviruses, molecular promoters and amplification systems. Finally, we present the concept of 'in vivo pathology', which envisages using molecular imaging to accurately localize metastatic lesions based on the molecular signature of the disease.Trends in Molecular Medicine 07/2009; 15(6):254-62. DOI:10.1016/j.molmed.2009.04.004 · 10.11 Impact Factor
Article: Titration of Variant HSV1-tk Gene Expression to Determine the Sensitivity of F-18-FHBG PET Imaging in a Prostate Tumor[Show abstract] [Hide abstract]
ABSTRACT: Because of its high selectivity and specificity for the imaging reporter probe 9-(4-(18)F-fluoro-3-[hydroxymethyl]butyl)guanine ((18)F-FHBG), the herpes simplex virus type 1 thymidine kinase (HSV1-tk) variant sr39tk is actively being studied as a PET reporter gene. We recently demonstrated the capability of using a prostate-specific transcriptional amplification PET reporter vector, AdTSTA-sr39tk, to target prostate cancer lymph node metastasis. However, one area that warrants further study is the examination of the sensitivity of PET by determining the minimum percentage of cells expressing the sr39tk transgene needed for detection. Addressing this question could determine the sensitivity of vector-mediated sr39tk PET in cancer-targeting strategies. DU-145, PC-3, and CWR22Rv.1 prostate cancer cell lines (a total of 1 x 10(6) cells) were studied, of which 7%, 10%, 25%, 50%, or 70% were transduced with the lentiviral vector constitutively expressing HSV1-sr39tk-IRES-enhanced green fluorescent protein (EGFP). Cells were subcutaneously implanted into the left shoulder of severe combined immunodeficient mice and evaluated. Tumor cells comparably transduced with an EGFP control vector were implanted on the right shoulder. Mice were imaged using PET with (18)F-FHBG at 8, 15, and 22 d after tumor implant. On day 23, tumors were isolated and analyzed for sr39tk transgene expression by quantitative reverse-transcriptase polymerase chain reaction (RT-PCR), Western blotting, immunohistochemistry, and flow cytometry for EGFP expression. Results showed a linear relationship between the level of sr39tk expression and the quantity of tracer accrual in DU-145, with the minimal value for PET detection at 10%. The magnitude of tracer retention in sr39tk-expressing cells was amplified over time as the tumor grew. Protein levels in the stepwise titration increased with the percentage of sr39tk-transduced cells. The stepwise titration of prostate cancer cells transduced with the lenti-CMV-sr39tk-IRES-EGFP determined the minimum number of sr39tk-expressing tumor cells necessary to be detected by PET using the (18)F-FHBG reporter probe. Furthermore, PET signal correlated well with traditional methods of protein evaluation such as flow cytometry, quantitative RT-PCR, Western blotting, and immunohistochemistry. Unlike the traditional methods, however, the use of PET is noninvasive and will be more advantageous in clinical situations.Journal of Nuclear Medicine 05/2009; 50(5):757-64. DOI:10.2967/jnumed.108.058438 · 5.56 Impact Factor
Article: Adenovirus-mediated gene expression imaging to directly detect sentinel lymph node metastasis of prostate cancer.[Show abstract] [Hide abstract]
ABSTRACT: The accurate assessment of nodal involvement in prostate cancer is crucial to planning treatment, yet there is a shortage of noninvasive imaging techniques capable of visualizing nodal lesions directly. This study demonstrates the feasibility of using recombinant human adenoviral vectors to detect nodal metastases in a human prostate cancer model. This was achieved by the prostate-restricted expression of optical and positron emission tomography (PET) imaging reporter genes by the viral vector coupled with the innate lymphotropic properties of adenovirus. We show that peritumoral administration of these vectors results in the direct detection of reporter gene expression in metastatic lesions within sentinel lymph nodes. Notably, this approach parallels the current lymphoscintigraphy method but enables the direct PET visualization of sentinel lymph node metastases, eliminating the need for invasive lymphadenectomy. These findings may lead to more effective diagnostic and therapeutic strategies for individuals with advanced-stage prostate cancer.Nature medicine 08/2008; 14(8):882-8. DOI:10.1038/nm.1727 · 28.05 Impact Factor
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ABSTRACT: Prostate cancer dissemination is difficult to detect in the clinic, and few treatment options exist for patients with advanced-stage disease. Our aim was to investigate the role of tumor lymphangiogenesis during metastasis. Further, we implemented a noninvasive molecular imaging technique to facilitate the assessment of the metastatic process. The metastatic potentials of several human prostate cancer xenograft models, LAPC-4, LAPC-9, PC3 and CWR22Rv-1 were compared. The cells were labeled with luciferase, a bioluminescence imaging reporter gene, to enable optical imaging. After tumor implantation the animals were examined weekly during several months for the appearance of metastases. Metastatic lesions were confirmed by immunohistochemistry. Additionally, the angiogenic and lymphangiogenic profiles of the tumors were characterized. To confirm the role of lymphangiogenesis in mediating metastasis, the low-metastatic LAPC-9 tumor cells were engineered to overexpress VEGF-C, and the development of metastases was evaluated. Our results show CWR22Rv-1 and PC3 tumor cell lines to be more metastatic than LAPC-4, which in turn disseminates more readily than LAPC-9. The difference in metastatic potential correlated with the endogenous production levels of lymphangiogenic growth factor VEGF-C and the presence of tumor lymphatics. In agreement, induced overexpression of VEGF-C in LAPC-9 enhanced tumor lymphangiogenesis leading to the development of metastatic lesions. Taken together, our studies, based on a molecular imaging approach for semiquantitative detection of micrometastases, point to an important role of tumor lymphatics in the metastatic process of human prostate cancer. In particular, VEGF-C seems to play a key role in prostate cancer metastasis.International Journal of Cancer 11/2007; 121(10):2153-61. DOI:10.1002/ijc.22900 · 5.01 Impact Factor
Article: Differential biodistribution of adenoviral vector in vivo as monitored by bioluminescence imaging and quantitative polymerase chain reaction.[Show abstract] [Hide abstract]
ABSTRACT: A better understanding of the in vivo biodistribution of adenoviral vectors would enable the researcher to anticipate potential side effects due to off-targeted site of transduction, and aid in the strategic design of gene therapy. We combined real-time polymerase chain reaction with in vivo optical imaging to examine viral transduction in liver, lung, spleen, kidney, prostate, and lymph nodes. A replication-deficient serotype 5 adenoviral vector expressing the firefly luciferase gene under the control of a constitutive cytomegalovirus promoter was administered in vivo via different routes. Intravenous and intraperitoneal injections resulted in greatest gene expression and viral DNA in the liver, whereas intraperitoneal injections led to a greater extent of gene delivery to the prostate. Although prostate-directed injection resulted in dominant gene expression in the targeted site, leakage of the vector to other organs was also observed. Vector injection into the lymphatic-rich paw tissue or the subcutaneous tissue of shoulder or chest followed the expected lymphatic drainage pattern, resulting in the accumulation of viral vector in ipsilateral brachial and axillary lymph nodes. Collectively, this study demonstrates that each tissue retains various amounts of adenoviral vector, depending on the route of administration. This knowledge is useful in the strategic design and implementation of adenovirus-mediated gene therapies.Human Gene Therapy 01/2007; 17(12):1262-9. DOI:10.1089/hum.2006.17.1262 · 3.62 Impact Factor
Article: Specific targeting of gene therapy to prostate cancer using a two-step transcriptional amplification system.[Show abstract] [Hide abstract]
ABSTRACT: Significant advances in gene therapy have been made as a result of the improvement of gene delivery systems, discovery of new therapeutic genes, better understanding of mechanisms of disease progression, exploration and improvement of tissue-specific gene regulatory sequences, and development of better prodrug/enzyme systems. This review discusses adenoviral-based and prostate-specific cancer gene therapy--emphasizing tissue-specific promoter choices to increase gene therapy safety and specificity--and the development of prostate-targeted vectors, with a focus on the two-step transactivation system for amplifying gene expression, specifically in prostate cancer cells. Several examples will be discussed for the scientific basis and therapeutic applications. In addition, prostate cancer gene therapy clinical trials and future directions in this field will also be described briefly.Future Oncology 07/2006; 2(3):391-406. DOI:10.2217/147966184.108.40.2061 · 2.61 Impact Factor
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ABSTRACT: Gene-based therapy is a promising and flexible therapeutic approach to manage diverse types of cancer. The lack of convincing therapeutic success of current gene therapy protocols in part, can be attributed to the inability to monitor gene expression at the targeted site in the living subject. Linking molecular imaging to gene therapy will enable real-time assessment of the therapeutic process and the refinement of treatment protocols. This review will cover two common imaging modalities, positron emission tomography (PET) and bioluminescence imaging (BLI), used in pre-clinical and clinical gene therapy applications. Strategies to develop more specific and robust cancer gene therapy and imaging approaches will be discussed. Coupling PET to gene therapy of cancer has already been implemented in several clinical studies. This approach would help to improve the efficacy and safety of future gene therapy clinical trials.Current Gene Therapy 01/2006; 5(6):607-18. DOI:10.2174/156652305774964695 · 4.91 Impact Factor
Article: 1051. Prostate Cancer-Targeted Suicide Gene Therapy Achieved Effective Tumor Destruction While Safeguarding Against Systemic Toxicity[Show abstract] [Hide abstract]
ABSTRACT: Cancer gene therapy based on tissue-restricted expression of cytotoxic gene should achieve superior therapeutic index over an unrestricted method. This study compared the therapeutic effects of a highly augmented, prostate-specific gene expression method to a strong constitutive promoter-driven approach. Molecular imaging was coupled to gene therapy to ascertain real-time therapeutic activity. The imaging reporter gene (luciferase) and the cytotoxic gene (herpes simplex thymidine kinase) were delivered by adenoviral vectors injected directly into human prostate tumors grafted in SCID mice. Serial bioluminescence imaging, positron emission tomography (PET) and computed tomography (CT) revealed restriction of gene expression to the tumors when prostate-specific vector was employed. In contrast, administration of constitutive active vector resulted in strong signals in the liver. Liver serology, tissue histology, and frail condition of animals confirmed liver toxicity suffered by the constitutive active cohorts, while the prostate-targeted group was unaffected. The extent of tumor killing was analyzed by apoptotic staining and human prostate marker (PSA). Overall, the augmented prostate-specific expression system was superior to the constitutive approach in safeguarding against systemic toxicity, while achieving effective tumor-killing. Integrating non-invasive imaging into cytotoxic gene therapy will provide a useful strategy to monitor gene expression and therapeutic efficacy in future clinical protocols.Molecular Therapy 08/2005; DOI:10.1016/j.ymthe.2005.07.598 · 6.43 Impact Factor
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ABSTRACT: Due to the exceptionally limited treatment options currently available for recurrent hormone refractory prostate cancer (HRPC), gene therapy can provide a tool to fight this Herculean disease. To develop a safe and efficient vector-based gene delivery approach, we employed a prostate-targeted gene expression system. The combination of a modified PSA promoter/enhancer and two-step transcriptional amplification (TSTA) system enabled us to achieve robust expression of transgenes in our vectors while maintaining prostate specificity. Furthermore, integration of an efficient adenovirus-mediated gene delivery approach and noninvasive optical imaging allowed us to continuously monitor and evaluate our system on human prostate cancer mouse xenografted models (Sato, et al. Mol Ther, '03). We have also shown that the TSTA system can be usable for clinically-translatable mPET radionuclide imaging, applicable to HRPC where androgen receptor (AR) is expressed (Sato, et al. Clin Cancer Res, '05), and that the suicide gene therapy approach with the TSTA system exhibited promising results, as well as remarkably lower liver toxicity compared to conventional CMV promoter (Johnson et al. submitted).To further explore therapeutic approaches to utilize the TSTA system, we have taken an oncolytic approach to eradicate prostate cancer. Previously we had confirmed that two distinct genes can be expressed from a single construct because of the structural nature of the TSTA system. A pair of firefly luciferase (FL) and HSV-1 sr39tk genes flanking five repeats of GAL4 binding sites were both expressed from a single adenovirus vector simultaneously. We successfully obtained both optical and mPET imaging on the same animal by this bidirectional vector. Based on this, we developed an oncolytic adenovirus utilizing the TSTA system. In this new vector, both E1A and E1B driven by GAL4 binding sites will make the virus replication competent by the GAL4VP16 activator expressed in trans. Therefore, where the TSTA is active, E1A and E1B will be expressed, and this should lead to oncolytic adenoviral replication. In a preliminary study, virus replication was suggested by the increase of the FL activity from the AdTSTA-FL co-infected in the AR+ prostate cell line, LNCaP, following the transfection of the plasmid with E1A and E1B flanking two GAL4 repeats (G2). However, no increase of the FL activity was seen with the E1A and E1B plasmid with no GAL4 repeat (G0), possibly because of no virus production. The viral production was actually confirmed by a plaque formation in the transfection study with a G2 or G4 driving E1 proteins but was not for G0 in addition to the expression of E1 proteins. Viral replication was also confirmed by co-infection study using G4 adenovirus but not with G0 adenovirus. Currently we are working on analyzing G0, G2, and G4 adenovirus to see oncolytic activity and constructing all-in-one TSTA oncolytic adenoviruses. We foresee that this oncolytic approach based on the TSTA system can be another promising gene therapy strategy for the advanced stages of prostate cancer.Molecular Therapy 08/2005; 11. DOI:10.1016/j.ymthe.2005.07.555 · 6.43 Impact Factor
Article: Functionality of androgen receptor-based gene expression imaging in hormone refractory prostate cancer[Show abstract] [Hide abstract]
ABSTRACT: A highly augmented, prostate-specific two-step transcriptional amplification (TSTA) method was developed with the ultimate goal of delivering an effective and safe gene-based treatment to prostate cancer patients. Because very limited treatment options are available for recurrent hormone refractory prostate cancer (HRPC), it is imperative to assess whether the prostate-specific antigen (PSA) promoter-based TSTA gene therapy will be functional in HRPC. We tested the TSTA-driven adenovirus vector on three androgen-dependent and six HRPC models. Real-time gene expression was monitored by both optical imaging and the combined modality of positron emission tomography (PET) and computed tomography. The TSTA-driven firefly luciferase expressing adenoviral vector was active in all androgen receptor (AR)-expressing HRPC models, but inactive in AR- and PSA-negative lines. Interestingly, the TSTA-mediated gene expression was induced by hydrocortisone in MDA PCa 2b, a cell line with mutated AR that possesses altered ligand specificity. In animal models, the TSTA-mediated optical signal was more robust in the HRPC than androgen-dependent tumors. In a parallel trend, a TSTA vector that expresses the herpes simplex virus thymidine kinase PET reporter gene also displayed more robust PET signal in the HRPC tumor. The activity of TSTA system is AR dependent and it recapitulates the functional status of endogenous AR. These data support the conclusion that AR function is activated in HRPC despite castrated levels of androgen. Together with the fact that majority of recurrent prostate cancers express AR and PSA, we foresee that the TSTA approach can be a promising gene therapy strategy for the advanced stages of prostate cancer.Clinical Cancer Research 06/2005; 11(10):3743-9. DOI:10.1158/1078-0432.CCR-04-1990 · 8.19 Impact Factor
Article: Lentiviral vector retargeting to P-glycoprotein on metastatic melanoma through intravenous injection[Show abstract] [Hide abstract]
ABSTRACT: Targeted gene transduction to specific tissues and organs through intravenous injection would be the ultimate preferred method of gene delivery. Here, we report successful targeting in a living animal through intravenous injection of a lentiviral vector pseudotyped with a modified chimeric Sindbis virus envelope (termed m168). m168 pseudotypes have high titer and high targeting specificity and, unlike other retroviral pseudotypes, have low nonspecific infectivity in liver and spleen. A mouse cancer model of metastatic melanoma was used to test intravenous targeting with m168. Human P-glycoprotein was ectopically expressed on the surface of melanoma cells and targeted by the m168 pseudotyped lentiviral vector conjugated with antibody specific for P-glycoprotein. m168 pseudotypes successfully targeted metastatic melanoma cells growing in the lung after systemic administration by tail vein injection. Further development of this targeting technology should result in applications not only for cancers but also for genetic, infectious and immune diseases.Nature Medicine 04/2005; 11(3):346-52. DOI:10.1038/nm1192 · 28.05 Impact Factor
Article: 975. TSTA Mediated Multi-Modality Gene-Expression Imaging in Hormone Refractory Prostate Cancer[Show abstract] [Hide abstract]
ABSTRACT: Molecular Therapy (2004) 9, S373–S373; doi: 10.1016/j.ymthe.2004.06.948 975. TSTA Mediated Multi-Modality Gene-Expression Imaging in Hormone Refractory Prostate Cancer Makoto Sato1, Mai Johnson1, Liqun Zhang2, Sanjiv S. Gambhir3,4, Michael Carey2,3 and Lily Wu1,31Urology2Biochemistry3Crump Institute for Molecular Imaging, UCLA School of Medicine, Los Angeles, CA4Department of Radiology and Bio-X Program, Stanford Univ, Stanford, CAMolecular Therapy 04/2004; DOI:10.1016/j.ymthe.2004.06.948 · 6.43 Impact Factor
Article: Optimization of adenoviral vectors to direct highly amplified prostate-specific expression for imaging and gene therapy.[Show abstract] [Hide abstract]
ABSTRACT: Gene expression-based imaging coupled to gene therapy will permit the prediction of therapeutic outcome. A significant challenge for successful gene therapy is to achieve a high-level of specific gene expression; however, tissue-specific promoters are weak. We postulate that if the weak activity of tissue-specific promoters can be amplified to the levels of strong viral promoters, which have been successful in preclinical scenarios, while retaining specificity, the therapeutic index of gene therapy can be greatly augmented. With this in mind, we developed a two-step transcriptional activation (TSTA) system. In this two-tiered system, a modified prostate-specific antigen promoter was employed to drive a potent synthetic transcriptional activator, GAL4-VP2. This, in turn, activated the expression of a GAL4-dependent reporter or therapeutic gene. Here we demonstrate that recombinant adenoviral vectors (Ads) in which we have incorporated prostate-targeted TSTA expression cassettes retain cell specificity and androgen responsiveness in cell culture and in animal models, as measured by noninvasive optical bioluminescence imaging. We investigated the mechanism of TSTA in different adenoviral configurations. In one configuration, both the activator and the reporter components are inserted into a single Ad (AdTSTA-FL). The activity of AdTSTA-FL exceeds that of a cytomegalovirus promoter-driven vector (AdCMV-FL), while maintaining tissue specificity. When the activator and reporter components are placed in two separate Ads, androgen induction is more robust than for the single AdTSTA-FL. Based on these findings, we hope to refine the TSTA Ads further to improve the efficacy and safety of prostate cancer gene therapy.Molecular Therapy 12/2003; 8(5):726-37. DOI:10.1016/j.ymthe.2003.08.016 · 6.43 Impact Factor
Top Journals
- Molecular Therapy (4)
- Nature Medicine (2)
- Cancer Research (1)
- The Journal of Urology (1)
- Journal of Nuclear Medicine (1)
University of California, Los Angeles
Los Ángeles, California, United States
- • Institute for Molecular Medicine
- • Department of Molecular and Medical Pharmacology
- • Department of Urology
Stanford UniversityPalo Alto, California, United States
Los Angeles Biomedical Research Institute at Harbor-UCLA Medical CenterTorrance, California, United States
Comprehensive Cancer Centers of NevadaLas Vegas, Nevada, United States


text:
OAKLAND (AP) - In three short years, Sean Doolittle transformed himself from a top first base prospect into a shut-down reliever.
SAN JOSE (AP) - Tomas Hertl and Raffi Torres both scored in their first game together as teammates and the San Jose Sharks jumped on top of Jonathan Quick and the rival Los Angeles Kings with a 6-3 victory in their playoff opener Thursday night.
MIAMI (AP) - Finally, the NBA playoffs are set.
Jozy Altidore's disappointing first season with England's Sunderland has less than a month remaining, and it appears as though the American's struggles for playing time aren't finished.
Duke freshman Jabari Parker is entering the NBA draft, and there's a strong chance he'll be the No. 1 pick.
SAN FRANCISCO (AP) - Hyun-Jin Ryu pitched for his country, doing anything he could from afar to lift spirits in South Korea after all the lives lost during a devastating ferry accident.
MIAMI (AP) - The smugglers who helped Los Angeles Dodgers star Yasiel Puig leave Cuba on a speedboat have made death threats against him and against a Cuban boxer who says he defected with Puig, according to court documents.
DENVER (AP) - Jordan Crawford scored a career-high 41 points and Harrison Barnes added 30 as a squad of Golden State backups beat the Denver Nuggets 116-112 Wednesday night.
SAN JOSE (AP) - For more than a decade there have been no questions about who will start in goal for the San Jose Sharks come playoff time.
ANAHEIM (AP) - Chris Iannetta homered with two outs in the 12th inning against Drew Pomeranz, giving the Los Angeles Angels a 5-4 victory over the Oakland Athletics on Wednesday night.
SAN FRANCISCO (AP) - Pablo Sandoval singled in the go-ahead run in the seventh inning, lifting the San Francisco Giants to a 2-1 victory over the rival Los Angeles Dodgers on Wednesday night and into first place in the NL West.
NEW YORK (AP) - The New York Yankees honored Nelson Mandela on Wednesday night during their rain-delayed celebration of the legacy of Jackie Robinson, another icon of social change.
SACRAMENTO (AP) - Archie Goodwin scored 12 of his career-high 29 points in the fourth quarter and the Phoenix Suns defeated the Sacramento Kings 104-99 Wednesday night in the season finale for both teams.
BOSTON (AP) - Bradley Beal scored 27 points and Washington clinched the No. 5 seed in the Eastern Conference with a 118-102 win over the Boston Celtics on Wednesday night.
ANAHEIM (AP) - Ryan Getzlaf had a goal and an assist, Frederik Andersen made 32 saves to win his NHL playoff debut, and the Anaheim Ducks opened the postseason with a 4-3 victory over the Dallas Stars on Wednesday night.
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LOS ANGELES (AP) - Los Angeles Clippers forward Matt Barnes was fined $50,000 by the NBA on Friday for directing inappropriate language at a courtside fan who turned out to be Houston star James Harden's mother.
NEW YORK (AP) - Ryan McDonagh scored 9:37 into overtime and the New York Rangers kept their Stanley Cup hopes alive with a 2-1 victory over the Washington Capitals on Friday night in Game 5 of the Eastern Conference semifinal.
PONTE VEDRA BEACH, Fla. (AP) - The biggest star at The Players Championship was not Rory McIlroy or Jordan Spieth playing together in the morning. And it wasn't Phil Mickelson and Tiger Woods in back-to-back groups in the afternoon.
CHICAGO (AP) - There was a time when Jimmy Butler was kicked out of his home as a teenager with no place to go. He has come a long way since then.
SOUTH LAKE TAHOE, (AP) - Mark Cavendish and Kristin Armstrong will highlight the Tour of California.
WASHINGTON (AP) - John Wall has five non-displaced fractures in his left wrist and hand, and the All-Star guard's playing status has not been determined.
NEW YORK (AP) - Floyd Mayweather said in an upcoming interview with Showtime that at this moment he's not interested in a rematch with Manny Pacquiao "because he's a sore loser and he's a coward."
ST. PAUL, Minn. (AP) - Corey Crawford made 34 saves, Patrick Kane had a goal and an assist, and the Chicago Blackhawks advanced to the Western Conference finals with a 4-3 victory over the Minnesota Wild on Thursday night for a four-game sweep.
SALEM, Mass. (AP) - New England Patriots quarterback Tom Brady said Thursday night the scandal surrounding his use of deflated footballs during the NFL playoffs last season hasn't detracted in any way from his Super Bowl title.
SAN FRANCISCO (AP) - On a night when he allowed more hits than any San Francisco pitcher in 47 years, two stood out to Tim Hudson.
MINNEAPOLIS (AP) - On a day when Drew Pomeranz had difficulty finding the plate, he sure could have used some help from his defense to grind through the series finale against the Minnesota Twins.
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text:
Hanukkah: 5 Holiday Lessons to Unwrap!
The Festival of lights
The Festival of lights
But the needed oil was eight days away. It would be easy to despair, after years of fighting and now realizing they were lacking the resources needed to move forward. But, as the story is told, the Maccabees decided it had been too long since the eternal light had been ignited and so they took a first step. Despite having only enough oil for one day, they committed themselves to starting the process of rededicating themselves and the Temple by starting where they were, and taking it one day at a time.
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Inspiring Stories That Made it to the Big ScreenHere are seven inspiring true stories that made it to the big screen.
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guitarist,gamer,funny thats all anyone really needs to know to get along with me.
No blog entries have been posted here. Boring yes, but as confucius says - if you have nothing good to say, why say anything at all? Check back later, perhaps something exciting will pop-up and be worth blogging about, like season 3 of the teletubbies.
Speak your mind, rant about games, mods and the development scene, it's simple and who knows maybe someone will read it and agree. It will feel right!


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Tel: (410)757-5000
School Overview
|School Membership(s)School Assoc.
|Other school association(s)
|Religious Affiliation
|Grades Offered
|Grades 7 - 8
Student Body
|Total Students
|19 students
|Student Body Type
|Students by Grade
Academics and Faculty
|Total Classroom Teachers
|1 teachers
|Student : Teacher Ratio
National avg.: 13:1
Nearby Schools:
All Schools | High Schools | Elementary Schools | Preschools | Public Schools
Show me:
- School Location Miles Students Grades
AnnapolisThe Harbour School At Annapolis
1277 Green Holly Drive
Annapolis, MD 21409
(410)974-4248Special Education School1.2 mi | 170 students | Gr. 1-12
AnnapolisCape Kids Corner
857 Chestnut Tree Drive
Annapolis, MD 21401
(410)757-2271Special Program Emphasis (Methodist)1.3 mi | 76 students | Gr. PK-5
AnnapolisIn Faith Christian School
657 Broadneck Rd
Annapolis, MD 21409
(410)757-7680Daycare / Preschool (Methodist)1.8 mi | 19 students | Gr. K
ArnoldArnold Christian Academy
365 Jones Station Road
Arnold, MD 21012
(410)544-1882(Christian)1.8 mi | 33 students | Gr. K-8
ArnoldCollege Parkway's Creative Beginnings Preschool
Po Box 859 301 College Parkway
Arnold, MD 21012
(410)647-9224Daycare / Preschool (Baptist)2.1 mi | 65 students | Gr. NS-PK
School Reviews
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Updated June 28, 2015
When it comes to choosing a private school, there are so many choices to consider that the project can be a huge challenge to most of us. We offer some help and advice to help you get started.
Sending your child to a foreign language school makes good sense when you have been posted to this country from abroad and in other circumstances as well.
Do you need financial aid? Not sure? Confused by how financial aid works? Here are some answers.


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I made the speculative add in the opening week of my fantasy league, and was roundly mocked. Who's mocking now, fellas??
Originally written on IT IS HIGH, IT IS FAR, IT IS caught | Last updated 5/12/13
Republican or Democrat, Vegan or Carnivore, Peanut Butter or Jelly - whatever your inclination - tonight, let us unite as obnoxious Yankee fans in one mighty chorus: Altogether, everybody:"VERNON WELLS IS OUR LEFT-FIELDER, AND HE IS BATTING THIRD."Note: This will not change when a certain Man of Grandeur emerges from the fracking ponds of Pennsylvania. This will not change when the Rod of A-Hip slithers up from Florida, or when Tex or the Captain, or even Tennille arrives. This will only change when Vernon Wells decides he isn't the Yankee left-fielder, batting third.I was wrong on Vernon Wells. We all were. Could he be the player version of Joe Torre - the guy who looked tanked until he reached the Yankees? Two months ago, Wells was run out of LA with the Angels paying half his tab, headed to a Yankee fan-base that viewed his presence as the ultimate sign of the Yankees impending apocalypse. Well, he's hit, fielded and stolen bases - been every bit the player he was once projected...
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Jason Cohen
Senior editor Jason Cohen has written for Texas Monthly since 1995 (and texasmonthly.com since its very first iteration). His 1997 story "The Ice Bats Cometh," about minor league hockey in Texas, was the basis of his book Zamboni Rodeo (Greystone Press, 2001). He also penned the magazine's first-ever Matthew McConaughey story, in August 1996. The co-author of Generation Ecch! (Fireside Books, 1994) and co-editor of SXSW Scrapbook (Essex/University of Texas Press, 2011), he has also written for such publications as Rolling Stone, SPIN, Details, the Austin Chronicle, the Austin American-Statesman, Portland Monthly, and Cincinnati magazine. His 1995 Rolling Stone cover story on the band Hole prompted Courtney Love to yell at him from the stage at Lollapalooza in Austin, while his 2007 profile of the Portland strip club Mary's won a Sex-Positive Journalism Award. As one of the two primary writers for the TM Daily Post, Cohen wrote approximately 500 stories for Texas Monthly in 2012. He has been a blogger since 2002 and been known to maintain as many as five Twitter accounts.
The Republican U.S. Senate candidate had some interesting things to say during his first appearance on the Sunday talk shows as a national political figure.
The Six Flags Fiesta Texas thrill ride, which at one time was the tallest, steepest, fastest wooden rollercoaster in the country, shut down operations Sunday.
The Top Chef winner tells Eater about his "flagship" restaurant, as well as plans for two new East Side Kings, including one near the University of Texas.
A Killeen woman with a history of domestic violence charges allegedly tried to hit her boyfriend with a "pink steel stripper pole."
Heart Stops Beating, the Sundance-featured short film about the Texas Heart Institute's unprecedented "continuous flow device," is reworked into a longer and more detailed version, now called Flatline.
The former Dallas Stars player and his wife of five years, Willa Ford, announced that they're divorcing.
Is this the Jerry World of high school football? The Eagles open the 18,000-seat facility in style August 31, when Southlake Carroll comes to visit.
The U.S. Senate candidate's national profile rises further with a speaking slot at the Republican National Convention in Tampa. Too bad that he can't ever run for president. Or can he?
How much can go wrong trying to field a single bunt? Earlier this week against the Nationals, your 36-77 "Lastros" put on quite the (gong) show.
The San Antonio-native's star performance for the silver medal-winning U.S. Olympics volleyball team was accompanied by two weeks of cheap Twitter jokes.
Mary Gonzalez of El Paso has been called the first lesbian member of the Texas legislature, but she tells the Dallas Voice that she prefers "pansexual."
During an exit interview with NBC's Chuck Todd, Kay Bailey Hutchison talked about Rick Perry, Mitt Romney, and "RINOs"—Republicans in Name Only.
The season premiere of the Travel Channel's Bizarre Foods came to Central Texas, with Andrew Zimmern hitting Kreuz, Lambert's, Black's, Pig Vicious, Contigo and more.
Eljiah Earnheart's battle with a Mesquite Pee Wee football association makes national headlines.
Picking up a Houston Chronicle story, the Fort Worth Star-Telegram printed the name of Chron reporter Yang Wang as "Yank Wang." She took it in good stride.


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A capsule look at Cardinals-Giants series
Watch For:
— Timmy Time. Lincecum looked nothing like a two-time Cy Young winner when he posted the highest ERA in the National League in the regular season. That performance kept him out of the rotation in the first round, but he excelled in his new role as a reliever. He allowed one run, three hits and no walks in 6 1-3 innings, getting the win in Game 4. He could move back into the rotation in the NLCS after Zito lasted just 2 2-3 innings in his one start.
— Triples Alley. The Giants hit just 31 homers during the regular season at AT&T Park — six fewer than Barry Bonds hit himself there during his record-setting 2001 season. They made up for the lack of longballs with a major league-leading 57, including 29 at home with the spacious right-center field.
— Carp in Control. It seemed to be a foregone conclusion that Chris Carpenter wouldn't pitch in 2012, especially after the 37-year-old had a procedure in July to relieve nerve pressure in his pitching shoulder that caused numbness to the entire right side of his body. Winless in three starts in September, he pitched shutout ball to win his start in the NLDS. It was his first victory since Game 7 of the World Series last year, and re-established him as an October ace.


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today's leftovers:
- Get Involved in Gentoo Linux
- 2012: What a year for Linux
- Subtitle Editor: Handy for Captioning but Lacking Instructions
- Nouveau NVIDIA Driver Can Be Faster With Linux 3.8
- Raspberry Pi gets an open source educational manual
- Jono Bacon Bobblehead. One step closer to reality.
- KDE 4.10 Desktop Delayed Into February
- Rsync, It's GRRRRaphical!
- The Ubuntu Phone OS Doesn't Stand A Chance
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Android-powered BlackBerry Venice with a slide-out keyboard reportedly headed to AT&T
Reports from earlier this month that BlackBerry would soon launch a full fledged Android-powered smartphone are looking up. Ex-tipster who still occasionally tips/confirms new devices @Evleaks tweeted earlier today that a device called the BlackBerry Venice is headed to AT&T later this year. He specifically mentioned that this device would be powered by Android and that — here's the best part — it will feature a slide-out physical keyboard for QWERTY fans.
Top 5 Android phones — July 2015
Then we have our wild cards, two phones are included this quarter, and both deserve some sort of mention. One isn't available yet, while the other is about to receive a much anticipated successor. Without further ado, let's dive into the top 5 Android smartphones for July 2015. And please, as always, keep in mind that these are in no particular order; each phone has plenty of pros and cons.
Linux Kernel Gets a Patch for Dell Airplane Mode Switch
A patch has been submitted to the Linux kernel to fix a problem that was really bothering the users of Dell laptops, and that's the ability to use the airplane mode switch.
Today in Techrights


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AP1 outlines bold approach to hedge funds
Martin Källström, the portfolio manager responsible for hedge funds at AP1, believes that the Skr221bn ($33bn) Swedish pension fund's approach to hedge funds is very different to that of most other investors in the sector.
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Except for a few lonesome souls, most people hate traveling alone and prefer to travel with a group. Totally understandable, group travel allows you to enjoy your vacation better, as you talk to someone and be safer too.
Travel with Family and Friends
When travelling to the UK, we suggest that you travel with:
- A group
- Or with family and friends
Whoever you decide to travel with, get help from a travel agency to plan your trip or join a trip with a set itinerary.
Cost Advantage
Group travel will cost you less. Also, since everything is already laid out, you need not worry about:
- Booking a hotel
- Organizing your trip overall
Of course, there are tradeoffs as your itinerary is set, some free time may be blocked.
Local Agency
To get the best deal, book a trip with a local agency. They will:
- Contact their counterpart in London;
- To welcome you at the airport.
If not, book a ticket to London, then book a group tour when you arrive. Remember, not all travel agencies are alike, so book with an awesome, low-fussed tour operator.
Go Beyond Guidebooks
Choose a travel agency that allows you to enjoy the United Kingdom like the locals do:
- From pubs;
- To fish and chips.
Doing what the Britons do lets you go beyond guidebooks, straight into a world waiting to be uncovered.
Guest Transportation
The best group travel services make plans for guest transportation, from departure to arrival to and from destinations. In destination locations, a planner does these:
- Arranges for mode of transportation;
- Plans step-by-step itinerary;
- Makes lodging bookings;
- Buys visitor tickets to entertainment locations.
Effective travel plans are really a make-or-break responsibility for group travel organisers because they greatly affect client experiences.
Group Travel Tour Package
Generally, a group travel tour covers:
- Airfare costs
- Ground transportation
- Hotels/lodgings
- Food
- Event registration
However, it doesn't cover car rentals, telephone calls, access to the internet and other incidentals.
Though you can organise your trip to save money, most travel agencies are prepared to go the extra mile to accommodate your requirements, demands and cost issues as well.


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Question of the Week
6/24/12Author: Cary Hutto
Topics: Government and civics, PoliticsComments: 0
6/17/12Author: Cary Hutto
Topics: Arts and Culture, PoliticsComments: 0
6/10/12Author: Cary Hutto
Topics: 19th century, Arts and Culture, PoliticsComments: 0
6/3/12Author: Cary Hutto Comments: 0
5/28/12Author: Cary Hutto
Topics: 19th century, Science and MedicineComments: 0
5/20/12Author: Cary Hutto
Topics: 18th century, Government and civicsComments: 0
5/13/12Author: Cary Hutto Comments: 0
Legend says that in 1682, William Penn met with a group of Native Americans to form a treaty. Where did they meet?
5/6/12Author: Cary Hutto Comments: 1
4/30/12Author: Amanda Dean
Topics: 18th century, Arts and Culture, PhiladelphiaComments: 0
Memory Stream
Look for these history stories every Sunday in the Philadelphia Inquirer. The stories, called Memory Stream, are published in the Currents section of the newspaper.


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Money blog + Personal loans
A survey by the Consumer Finance Association says 93% of customers think payday lenders treat them with dignity and respect. But looking at the sample shows a flawed process
Credit licences take years to revoke, but there is a way to check if a loan company is on the OFT's watchlist
We are running a live advice clinic on Monday 9 January at 1pm to help answer your questions about borrowing, debt and how to deal with it
The average person still has a desire to spend, but the credit card companies and other lenders have turned off the supply
The consumer champion and founder of moneysavingexpert.com is being interviewed by the Guardian. But you can ask him your own questions
Just days after the high court ruling on mis-selling PPI policies Jill Insley received a pushy phonecall
Consumer Credit Directive will offer additional customer provisions, with further measures aimed at empowering borrowers, says minister
The public supports a rate cap, but it would be a simplistic measure to the complex problem
Interest rates: Time to introduce a capPatrick CollinsonWhy has the Lib-Dem coalition only banned excessive charges on credit cards?
Struggling with debt repayments? Our expert panel will be online to help from 1pm on 18 January


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|Nokia C6 Price Pakistan, Nokia C6 Symbian smartphone. 5 MP camera, Features 3G, 3.2″ TFT resistive touchscreen, WiFi, GPS, Bluetooth.Announced 2010, April.
Mobile Prices > Nokia Mobiles > Nokia C6
Nokia Mobile Phones Prices in Pakistan,Mobile Phone Prices in Pakistan,Nokia C6 Mobile Phone Price in Pakistan,C6 Nokia Mobile Phone Price in Pakistan,
Disclaimer. We can not guarantee that the information on this page is 100% correct > Read More
You have just read an article that category Nokia / Nokia Mobiles by title Nokia C6 Price in Pakistan Mobile Price & Specification. You can bookmark this page URL http://www.pakistanmobilephoneprice.com/2012/04/nokia-c6-mobile-price-in-pakistan.html.


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BILLY MADISON - special outdoor screening with BuzzFeed at 50 Kent
- Film, Media & Entertainment
- 50 Kent Ave, Brooklyn, NY, 11249
Spit'in Anger - New York City
Fathers Incorporated
Monday, March 3, 2014 from 7:00 PM to 9:30 PM (EST)
Spit'in Anger is the exploration into the uncharted space of fatherlessness. Through the story of one of America's premier leaders in the responsible fatherhood movement--Kenneth Braswell--we learn that the filmmaker and healer is about to receive a lesson in healing himself. Experts Dr. Jeff Gardere, Iyanla Vanzant. Judge Mablean Ephriam and Terrie Williams lay the foundation for one of most devastating social ills for black children in America; father absence. Today; 82.3% of African American children born since 1990, will live in a home without their biological fathers before their 18th birthday.
FILM IS FOR FAMILIES, MOTHERS, FATHERS, SONS, DAUGHTERS, SERVICE PROVIDERS, CLERGY, SOCIAL WORKERS! We can be reached at email@example.com or at 518-713-4755. You can also visit our website at www.spitinanger.com
Fathers Incorporated is a 501(c)3 not-for-profit (S) corporation and is dedicated to strengthening the community and family infrastructure by encouraging and enabling the positive involvement of fathers in the lives of their children. Our mission also includes skills training to strengten healthy relationships and marriage. We do this through the use of innovative social marketing and multi-media platforms, event planning, training, presentations, traditional communications, and product development.
In order to purchase these tickets in installments, you'll need an Eventbrite account. Log in or sign up for a free account to continue.


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University of Jammu, Jammu invites online application forms from the eligible candidates for admission into Post Basic Nursing in BEE ENN College of Nursing, Jammu for the Academic Year 2012 – 2013. Total No. of Seats: 40 under General category and 06 under Management quota Eligibility: 10 + 2 from a recognized Board / University, […]
admin September 1st, 2012
Posted In: Education
Tags: Administration Block, Application Forms, Basic Nursing, Community Health Nursing, Degree Course, Downloaded Application, Eligible Candidates, Fee Details, Govt College, Jammuuniversity, Last Date, Leprosy, Male Nurse, Management Quota, Nursing And Midwifery, Nursing Council, Ophthalmic Nursing, Psychiatric Nursing, Registration Council, Year 2012
Courses / Total No. of Seats: Mahir-e-Tib / Mahir-e-Jaharat (MD Unani) i. Amraz-e-Niswan wa Qabalat: 02 (01 ST and 01 UR) ii. Ilmul Saidla: 03 [01 UR (AIQ) and 02 UR (DUQ)] iii. Moalejat: 03 (01 OBC and 02 UR) iv. Munaful Aza: 02 (01 SC and 01 OBC) Ayurveda Vachaspati (MD Ayurveda) Course i. […]
admin September 14th, 2012
Posted In: Education
Tags: 30th September, Aiq, Ayurveda, Ayurvedic, Aza, Bank Draft, Bums, Ccim, Degree Courses, Deputy Registrar, Duq, Graduate Medical, Information Bulletin, Kriya, Mahir, Medical Entrance, Obc, Registrar University, Unani Medicines, University Of Delhi
The Andhra Pradesh State Level Police Recruitment Board (APSLPRB) has released results of AP Police Constables results here today… Details are as on follows…. In pursuance to the notification issued in 927/R&T/Rect.2/2011 dated 31-10-2011, 14358 number of candidates have been provisionally selected to the posts of SCT Police Constables (Civil/AR/SAR CPL/APSP), Constables (Men) in […]
admin August 31st, 2012
Posted In: Police
Tags: Amp, Andhra Pradesh State, ap police constable, ap police constable results, Apstatepolice, apstatepolice.org, Ar Men, constable results, Cpl, East Godavari, Eligible Candidates, Emergency Services, Fire Men, Firemen, http://apstatepolice.org, Krishna, Level Police, Police Constable, police constable results, Police Constables, Police Recruitment, Position Post, Recruitment Board, Rect, Srikakulam, West Godavari, www ap state police org, www.apstatepolice.org
Full Time Contract Specialist/Part Time Contract Specialist: 10posts Qualification: Post Graduate degree in concerned speciality from recognized university with three year experience after acquiring the PG Degree or PG diploma with 5years post diploma experience Fee: Demand Draft of RS.I00/- (Rs.25/- in case of SC/ST) drawn in favour of ESI Fund Account No.1 (no fee for woman […]
admin September 6th, 2012
Posted In: Others
Tags: Contract Specialist, Demand Draft, Esi, Favour, Full Time, Graduate Degree, Medical Superintendent, Nic, Part Time, Passport Size Photograph, Pg Diploma, Post Diploma, Post Graduate, Relevant Documents, Testimonials, Time Contract, Uploaded, Woman Candidate
Results of 5 Years Dual Degree Course in Management 2012-13 at South Campus, Bhiknoor, Telangana University, Nizamabad-503322. The following (Hall Ticket Nos.) candidates are qualified the Entrance Test held on 23-08-2012 at University College, Main Campus, Telangana University, Dichpally, Nizamabad. 025YDDC01 025YDDC15 025YDDC27 025YDDC40 025YDDC54 025YDDC02 025YDDC16 025YDDC28 025YDDC41 025YDDC55 025YDDC03 025YDDC17 025YDDC29 025YDDC43 025YDDC56 […]
admin September 1st, 2012
Posted In: Education
Tags: Admission Fee, Bam, Bm, Bonafide, Caste, Certificates, Degree Course, Dual Degree, Hall Ticket, Mam, Nizamabad, Passport Size Photos, Rupees, Telangana, Test Results, University Degree, Xerox, Xerox Copies
JAWAHARLAL NEHRU TECHNOLOGICAL UNIVERSITY HYDERABAD KUKATPALLY : HYDERABAD 500 085 5 Yrs. IDDMP (JNTUH – SWEDEN ) – 2012 LIST OF SUCCESSFUL CANDIDATES The following is the provisional list of candidates qualified in the entrance Examination for admission to the 5 Yrs. Integrated Double Degree Masters Programe-2012 […]
admin September 6th, 2012
Posted In: Results
Tags: Admission, Brackets, Counselling, Double Degree, Entrance Examination Results, Hall Ticket, Jawaharlal Nehru, Jawaharlal Nehru Technological University, Masters, Month Of August, Mou, Sweden, Ticket Numbers


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Below are top resources on SparkPeople for Living Green Lifestyle.
John Keats once wrote, "The poetry of the earth is never dead."
Poet or not, almost all of us have been awestruck by nature at one time or another. Whether it's running at sunset on a sandy white beach, walking alongside a cool tri... Read more
Wouldn't it be great to be inside the mind of a health and fitness expert, even just for a little while? Now you can! Below, your favorite SparkPeople experts share the secrets of their healthy lifestyles. Just like you, they have to deal with... Read more
Most people who "go green" bring reusable bags to the store, unplug electronics when they're not in use, and recycle more. Others move to more involved changes, like trading in for a hybrid, buying carbon offsets, or installing sola... Read more
A healthy lifestyle is an ideal that we all strive for. Eat right, exercise regularly, get enough sleep. It seems pretty simple, right? But, we all know that it's much more complicated than a few simple words. While you might be successful in one... Read more
Store shelves are drowning is books and magazines dedicated to the care and maintenance of your body. Your abs, your thighs – even your heart – get special attention. But what about your mind?
Why does it seem like the brain, y... Read more
Green Tea ... 3 cups of water in progress.... Read more
I actually live in Verrado which is on the foothills of the White Tank Mountains on I-10 & Verrado. Plenty of trails for hiking and plenty of parks for some SparksWorkouts (Cardio, Calisthenics, and maybe some Yoga).
Just some ideas, Any Interest?... Read more
I live in the Southwest Valley, but work in the PHX area. Usual work hours are 6AM-4:30 PM, Mon-Thur. I'm free all Fridays and weekends in the AM hours. I'm open to suggestions or ideas depending on your schedule. Let me know & have a great day.... Read more
Well, I pulled a whole garbage bag of green beans from my garden. Unfortunately they have a real tough outer skin, so they don't taste good at all. My carrots have the same problem.... Read more
Did any of you try green coffee? I've heard it's good so i bought some, haven't searched about the benefits, just curiosity, tastes like warm grass juice... Ehh not very fond of the taste n smell... Lol... Read more
Grean tea, chilled is really yum :) I didnt like the flavor until it found healthries (brand) had green teas with fruit. Blackcurrent is my favorite and has added vit c. I have added the overall benefits below :) .... Weight Loss. Green tea in... Read more
How do I live this healthy lifestyle? How do I keep positive??... Read more
If you're a vegetarian, vegan, or just interested in a cruelty-free lifestyle, join us here! Get tips for making the transition, dining out, cooking and more.... Read more
''Yikes! It is hard to believe, huh?! We lived through it and didn't realize how bad it was until we were on this side of it.''That is an exact quote from my wife about the pictures you see.I'm not sure if it's funny o... Read more
We round up the week's top stories in health, fitness, and more.Stay-Young Secrets From 10 Fit Grannies Who Rock (Shape.com) These ladies are older and wiser, and we should certainly follow their lead! Work Life Balance Isn't Merely A Wo... Read more


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For myself, I would give it more stars, because I got it for the visuals -- the landscapes, the costumes, the atmosphere -- and Rinko Kikuchi. Read morePublished 19 months ago by zillah975
It wasn't quite what I was expecting but it wasn't completely horrible. There could've been a little more to it than there was.Published 24 months ago by Kerry Ryan Maloney
If you're an MMORPG lover then you MUST watch this little gem. It's a relatively short production that borders on art flick with minimalist dialogue that serves a practical... Read morePublished on December 27, 2012 by Wiz
This movie is a terribly boring art house film. Don't let the cover fool you...or the trailer like it did me. Read morePublished on October 5, 2012 by Thadeus Buttons
Frist I like watching diffirent types of movies: But I be frank and steight to this one. Story line very, dull, it sets up a borning story line but don't really flow with the... Read morePublished on February 3, 2012 by Lone shark...
If you're a fan of the Japanese director's films then you'll like "Assault Girls" . The film is a bit short and could do with a better developed plot line the film , all in all ,... Read morePublished on January 27, 2012 by 124c41
I first saw this at New York Comic Con 2010. What was advertised in the program book about this movie was totally misleading (for shame!). Read morePublished on December 11, 2011 by Shelly S.
The premise of "Assault Girls" is actually very cool and could have been very interesting. Unfortunately the execution of the movie is not up to modern standards and looks more... Read morePublished on September 21, 2011 by C. Hill
Wow, this movie sucks as a whole. All the reviews on here were right. It's super slow and boring. There was a 10 min intro with no action and just talk about the background story,... Read morePublished on September 10, 2011 by S. Der


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All of the Stars Tried to Outshine One Another on Last Night's Red Carpet
Sex, Drugs, and Don Draper: Everything to Know About Mad Men in 2 Minutes
It's Cute Friends and Fun Vacations in This Week's Best Celebrity Candids
See Every Angle of January Jones's Multibraid and Blue Cat Eye
Famous Friends and Full House Round Out the Rebels Gala
January Jones Poses Nude in Racy Photo Shoot
Score January Jones's Exact Chanel Red Lipstick Shade
Mad Men TBT: When Betty and Don Draper Were Adorable and in Love
Now's the Time to Break Out Your Denim Jacket
January Jones, Is That You?
Celebaby Roundup!
The Striped Shirt? Not Going Anywhere


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|Viewing Single Post From: Spoilers for the Week of December 3rd
|Nov 20 2012, 01:24 AM
absolving Ej of rape? kidnapping? murder?
|Spoilers for the Week of December 3rd · DAYS: News, Spoilers & Discussion


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Originally written on Fox Sports | Last updated 8/1/12
On Thursday, having already helped her team claim the women's gymnastics team gold medal, Gabrielle Douglas will try to do what no American gymnast has done before: Add an individual all-around gold medal to her team gold. This is history in the making, a 16-year-old taking her own shot at Olympic immortality, and it would culminate a remarkable journey for a gymnast who has been ascending to the top of this sport since the spring. Douglas first flashed her growing possibilities in May in New York when she bested the score of the darling of American gymnastics, Jordyn Wieber, at the American Cup. She did not win because she'd competed as an alternate, and even then the whispers swirling around that success were that she only scored so well because it did not count. Once the pressure tightened its grip on her, the thinking went, she would falter. Then, at the U.S. Olympic trials in San Jose, where the pressure was absolutely immense, she took home the highest score. Suddenly the ...
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C&D Construction, Inc.
B-408930.2: Feb 14, 2014
Additional Materials:
- Full Report:
Ralph O. White
(202) 512-8278
(202) 512-4800
(202) 512-8278
Office of Public Affairs
(202) 512-4800
C&D Construction, Inc., of Cocoa, Florida, a small business, protests the award of a contract to RUSH Construction, Inc., of Titusville, Florida, under invitation for bids (IFB) No. W912EP-13-B-0007, issued by the Department of the Army, Corps of Engineers, for construction services to repair the Canaveral Lock East and West timber approach walls to restore the walls to an operational condition, at the Canaveral Lock and Barge Canal, in Brevard County, Florida. IFB Synopsis at 1. C&D argues that the Corps of Engineers should have rejected RUSH's bid as nonresponsive.
We sustain the protest.
Jun 30, 2015
- Precise Management, LLC--ReconsiderationB-410912.2
Jun 29, 2015
- Bode Aviation, Inc.B-411265
- Decisive Analytics CorporationB-410950.2,B-410950.3
Jun 26, 2015
- SageCare, Inc.B-411260
- Computer World Services Corporation; CompQSoftB-411216,B-411216.3,B-411216.4,B-411216.5
- WKF Friedman EnterprisesB-411208
Jun 25, 2015
- K-MAR Industries, Inc.B-411262,B-411262.2
Looking for more? Browse all our products here


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7X10 Journey To The Centre Of The TARDIS(Grading/Discussion)(SPOILERS)
View Single Post
April 29 2013, 12:28 AM
Mr. Adventure
Location: Mr. Adventure
Re: 7X10 Journey To The Centre Of The TARDIS(Grading/Discussion)(SPOIL
tomalak301 wrote:
They started this mystery of who Clara was, going all the way back to the premiere episode of the season, and I hope we get answers before the season is out and everyone is fawning over the 50th anniversary show. If there's only 2 episodes left, we're going to get some rushed stories to wrap up the season successfully.
I think it was always going to wrap up in that manner. Before the season started I thought maybe the Doctor was going to continue to encounter "Claras" and mysteries but that doesn't seem to be the case. Even if she had remembered her talk with the Doctor I don't see how it would've advanced the solving of the mystery, she'd just know he's trying to solve one. Not that it couldn't have added some spice to their interrelationship.
Slugboy wrote:
I was fairly forgiving about the scary zombies---after all, there needs to be something to scare the children into running behind the couch.
It always confuses me, though, that the camera crew unfocuses the camera whenever filming the creatures (whether the zombies this week or the monsters from Hide). I can understand using a fuzzy lens to film a "creature's-eye view" but why would someone's vision go fuzzy when looking at the creatures?
At least for me personally, I've found that approach to be pretty effective, the monsters this week and last have seemed more intense than usual.
Mr. Adventure
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Be ready for your new shiny Spring and Summer postcards!
What's New
Best Happy birthday wishes
Now you can wish a happy birthday to your friends or loved ones on the desired date with this images and phrases Happy Birthday
A complete collection of messages and congratulations happy birthday. Send a birthday card who want more, so this really is a happy birthday
You'll find classic, tender congratulations for your boyfriend or girlfriend and above all, many images as fun and funny to congratulate your birthday known
This app will also allow you:
* Like your favorites Happy birthday phrases and save them in the Favorites tab
* Like the different Happy birthday phrases to affect the ranking in the "most voted" tab
* Check the real time updated ranking to see which are the most voted/liked Happy birthday phrases by other users
* Easily browse Happy birthday phrases with an easy and friendly interface
* Receive a daily notification with a new image of Happy birthday phrases every day
Happy Birthday Cards
You can use it to wish a "Happy Birthday" to your most beloved ones: your friends, family and even your couple. This way you can even add funny birthday quotes of your own to make that day even more special and unique.
¿How can I use the Happy Birthday Cards app?
Once installed, next step is choosing whether you want to create your own happy birthday messages or just use some of the already created for this occasion.
If you want to create your own birthday postcards:
We've created a complete happy birthday pictures or images editor so you can create your own in a simple and fast way:
1- Choose your background: The first step is selecting the background you need for your creation. Use the arrows to choose the background you like the most and once you've made up your mind, tap on the background to select it.
2- Create your own happy birthday quotes: This second step allows you to insert the custom text you want to include on your birthday postcard. Just write a text and then choose the font type, size and even the text color, definitely a great way to create postcards with funny birthday wishes. You can move text position with your finger and place it wherever you prefer. Once you are done, tap on the arrow on top right to continue with the next steps.
3- Add icons: Through this step, you can add different types of icons or happy birthday images to your own creation. Tap on your favorite icons to add them to the image and then move them around the screen by using your finger until they are placed on the spot of your choice.
4- Send your happy birthday wishes: On this last step, you can send your birthday wishes to that person you want to congratulate on that special day. Don't worry, your congratulations will be automatically saved in the "My creations" section so you can view it and share it whenever you want to. Remember that you can use this postcards editor to create birthday invitations and send them out to your friends or relatives.
If you prefer using the designs offered by the app:
If you don't want to create your own postcard and prefer using already created ones, don't worry, we've created the best happy birthday designs so you can share them:
1- Choose the congratulations postcard: Select the one you liked the most, you can browse through all of them by using the arrows on screen.
2- Share the postcard: You can download it, save it as wallpaper, add it to your favorites and of course, share it with the person you want to congratulate through any instant messaging app, social networks, email, birthday sms or just through any app you may have installed on your smartphone or tablet.
Remember: You can access to your favorite designs or your own creation from the app main screen by tapping on upper left button.
We hope you enjoyed this app. We are always opened to suggestions on how to improve our service, so if you have any idea that can help us with this, don't hesitate to contact us. On the other hand, of you liked the app, we'd appreciate it if you could rate it positively, this will help us to keep the app available including improvements and new features.
Legal notice/Disclaimer:
All designs used on this app have been created by exclusive designers for Reticode, therefore the commercial use of any image included in the program is prohibited without the previous authorization of Reticode.
For some designs we have used images from public domain since they are not identified in any way that indicates the existence of exploitation rights reserved thereon.
Any natural or legal person who was the owner of any images contained herein, may accredit through firstname.lastname@example.com, committing to the immediate withdrawal of the image after verification, if necessary, ownership of the image or protected sentence.
Birthday Card
Over 100 professional designed birthday cards for you to choose.
* This app quality is assured as this app is available for iphone.
We hope you can send all the birthday card to your beloved and friends to express your best regards.
Step 1: Choose a birthday card.
Step 2: Share the birthday card.
Step 3: Send out the birthday card via Facebook, whatsapp, wechat, whatsapp, Email and other social media app.
Step 4: Waiting your beloved and friends to reply you!
-You can chat with the rest of people in this app and wishes each other happy birthday. The chat has 12 rooms from January to December.
Happy Birthday Card is one of the best app in the market!
Happy Birthday Images
Now you can wish a happy birthday to your friends or loved ones on the desired date with this card.
Very useful to use as personalized birthday invitation for both children and adults. You can also use it to compliment other special days, such as holding another holiday or important event.
Birthday Wishes
The main benefits of our app:
-Huge base of Birthday Wishes
-Friendly color and interface
-Simple usability
-Surprise effect(randomization function)
-Quick share function
-Working on every android device in the world
We sincerely hope that our Birthday Wishes app will be helpful for you.
Talking Ginger 2
Talking Ginger, the cutest little kitten, is growing up and wants you to celebrate his birthday with him. :-)
Join him for the best party ever - with awesome food and loads of games! Yes, you're invited to hang out with the most adorable kitten in the world!
- Talk to Ginger: Speak and he will repeat what you say in his cute voice.
- Play with Ginger: Cuddle, tickle or poke Ginger to see his funny reactions.
- Eat with Ginger: Feed him snacks or use the special 'meal time' button to eat together.
- Record Ginger: Record videos of Ginger having fun and share them with your friends.
- Celebrate with Ginger: Blow the party whistle for as long as you can.
- Play the mini-game: See how many birthday candles you can blow out before the timer runs out.
- Win free snacks: Get a free spin of the Fortune Wheel every 24 hours, or earn snacks within the app.
- Unlock rewards: Keep playing with Ginger to unlock more than 50 different jigsaw puzzle images.
This app is PRIVO certified. The PRIVO safe harbor seal indicates Outfit7 has established COPPA compliant privacy practices to protect your child's personal information. Our apps do not allow younger children to share their information.
This app contains:
- Promotion of Outfit7's products and contextual advertising
- Links that direct customers to our websites and other Outfit7 apps
- Personalization of content to entice users to play the app again
- Watching videos of Outfit7's animated characters via YouTube integration
- The option to make in-app purchases
Terms of use: http://outfit7.com/eula-android/
Privacy policy: http://outfit7.com/privacy-policy/
Birthday Wishes and cards
Lots of birthday wishes and birthday greeting cards.
Choose the most appropiate birthday message for the ocasion, choose one of the cute birthday cards and send it to your beloved ones.
- Birthday wish of the day.
- Favorite birthday quotes.
- Search birthday messages.
- 46 birthday card designs. You can import pics from your gallery or even take one with your phone to use as a birthday card.
- Customize color, style and size of the birthday card greeting.
- Send the Birthday card using Facebook, Whatsapp, E-mail, Messegner, etc.
Happy Birthday Cards
Customized birthday cards with messages
Write the congratulations note and share it with anybody you want
Choose the font and color you prefer
Write the greeting card in your own handwriting
All images have been acquired by this enterprise for exclusive use. These pictures have all rights reserved and they belong to their author.
Birthday Card
Wish You Happy Birthday with Beautiful Birthday Card!
All these Birthday Card are designed by professional designers. We hope you can send all the birthday card to your beloved and friends to express your best regards.
This is a very simple app.
Step 1: Choose a birthday card.
Step 2: Share the birthday card with your comment.
Step 3: Send out the birthday card.
Step 4: Waiting your beloved and friends to reply you!
Enjoy this app!
Greeting card, birthday card, happy birthday
Frases de Cumpleaños
* Para Guardar una imagen, pulsa sobre ella y aparece una ventana para poder descargarla.
* Para Compartir una imagen, primero descargarla, de lo contrario comparte el enlace.
***NOTA: Se recomienda instalar la aplicación con la conexión WIFI activada, ya que tienen que cargarse al iniciar la App todas las categorías (puede consumir ancho de banda 3G/4G de lo contrario).
Happy Birthday Frames
In addition you can also add birthday and party stickers related to your photos, apply cool effects and modify the picture to make it amazing.
You can add text and thus put a nice post card, with the font and color that you like more.
You'll use a lot of super easy to use tools to create photomontages to wish a happy birthday.
When finished you can send it by WhatsApp, Facebook, Twitter or any other application you have pressing a button.
You can even use any image as wallpaper greeting or save in the memory of your phone.
More details:
-Add Love frames, stickers or text to your photos.
-Use Effects to your photos.
-Save The images on your phone
-Use it as wallpaper
-Send by WhatsApp, Facebook or any other App.
-Simple And very easy to use.
-100% Free.
This application will be very useful for you to make super original Happy Birthday wish cards.
Feliz Cumpleaños
Las Frases y tarjetas mas increíbles para alegrar cumpleaños y celebraciones.
Hermosas frases y dedicatorias para felicitar en su cumpleaños a nuestros seres queridos.
Y puedes compartirlas por tu red social favorita
Puedes enviar tu imagen o postal de feliz cumpleaños por tu red social favorita a quién quieras felicitar en su día con una bonita tarjeta de felicitacion.
Pulsa el icono que esta en la esquina superior izquierda para hacer aparecer el menú
desliza tus dedos a derecha e izquierda sobre la pantalla de tu móvil para cambiar de imagen
Pulsa sobre el icono compartir para enviar tu imagen
Pulsa sobre el icono SD para guardar tus imágenes preferidas
Pulsa sobre el icono fondo y podrás poner tu imagen como fondo de pantalla.
- Sencillo, rápido, intuitivo, elegir y compartir.
- Envia gratis a tus amigos, contactos y familiares.
- La aplicación permite la instalación en la SD.
- Podrás guardar tus imágenes favoritas en la tarjeta SD.
- Pulsa sobre el icono compartir para enviar la postal a través de cualquier red social.
- Establece como fondo de escritorio la imagen que mas te guste con un solo clic.
- Esta aplicación contiene publicidad para sufragar los gastos de programación.
- Esta aplicación es Totalmente gratis.
Birthday SMS Collection
You can share SMS to your friends in just One click via Whatss app, Hike, Line , Messaging and more message apps
installed in your device.
Hindi SMS are available in both hindi and english fonts.
Feliz Cumpleaños
- Feliz cumpleaños tiene la opción de enviar la imagen con frases de cumpleaños por redes sociales con solo un click.
-Se puede guardar las imágenes como fondo de pantalla con solo un click.
Se irán añadiendo nuevas imágenes.
Algunas imágenes son de recopilación libre en la red, si crees que alguna infringe derechos de autor, ponte en contacto con nosotros acreditando la autoría de las mismas y serán retiradas.
★ Alles Gute zum Geburtstag Animierte Bilder
★ Happy Birthday, Karten, Geburtstagskuchen ...
► Um ein Bild herunterzuladen klicken Sie es und ein Fenster erscheint zum Download.
Happy birthday greetings
1. Write the name of the person you want to congratulate and the number of birthdays.
2. Enter your name to be displayed as the signature.
3. Choose the image or animation you want to appear on the card or upload a photo and select a phrase or write your own phrase.
Ready is finished!
Now you can send via a link, sharing for Whatsapp, Facebook, mail or internet platform ...
You can also send the birthday wishes via SMS to any mobile phone .
With reminders Facebook letting you know when is the birthday of your family or friends, you have no excuse not to congratulate, and what better way also easily and quickly be sent a greeting card birthday , which can customize without having to buy one, you can now design it yourself, because in our application can select a variety of images and phrases birthday.
Have sentences for each type of person, whether your raw / os, brothers / sisters, friends / spouses, parents, grandparents, children or your partner find an appropriate message to them.
And in less than one minute, you get a nice greeting to send to friends or family.
So simple and quick to create your own personalized card that will surprise you is the one who send.
We hope you enjoy this application to create birthday cards and we would like valorases us if you liked.
This application is on the web http://www.felicitaciondecumpleanos.com/
More from developer
Flowers Postcards
Be ready for your new shiny Spring and Summer postcards!
Get well Postcards
Be ready for your new shiny Spring and Summer postcards!
Memes postcards
Love Postcards
Be ready for your new shiny Spring and Summer postcards!
Ripple Price
Feel free to donate: rDLCE1RxnEB2HH62B6TaP4oBuA8H3zBeVm (Ripple wallet)
If you want me to add some gateway please leave a comment in Google Play.
Shiny Postcards
You get Birthday, Flowers, Love, Get Well, Wedding and Internet Memes pictures - all in one app!
Be ready for your new shiny Spring and Summer postcards!


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Kensington Court Hotel Notting Hill, London
- Free continental breakfast and WiFi
Main amenities
- 20 smoke-free guestrooms
- Restaurant
- 24-hour front desk
- Luggage storage
What's around
- In Paddington
- Royal Albert Hall (1.2 mi / 2 km)
Loved the quaint location. People were friendly. Close to a lovely pub. Was surprised…
one shared bathroom and 20 rooms. Need I say any more
Enter dates to check prices
Hotel Info: 866-539-8117from $109
- Deluxe Suite, 3 Bedrooms (7 adults)
- Deluxe Suite, 2 Bedrooms (5 adults)
- Deluxe Suite, 2 Bedrooms (6 adults)
- Deluxe Suite, 3 Bedrooms (6 people)
- Double Room, Ensuite
- Basic Triple Room, Shared Bathroom
- Deluxe Suite, 2 Bedrooms (7 adults)
- Basic Double Room, Shared Bathroom
- Family Room (5 adults)
- Deluxe Suite, 2 Bedrooms (4 adults)
- Triple Room, Ensuite
- Quadruple Room, Ensuite
Recommended hotels
At a glance
Key facts
Hotel size
- This hotel has 20 rooms
- This hotel is arranged over 5 floors
- Check-in time starts at 2:30 PM
- Check-out time is 11:00 AM
- Express check-in/out
Required at check in
- Credit card or cash deposit required
- Government-issued photo ID required
Travelling with others
- Pets not allowed
- Free WiFi in public areas
- Free WiFi in rooms
- No parking
Other information
- Smoke-free property
Nearby Three Star London hotels
In the hotel
- Free continental breakfast
- Restaurant
- 24-hour front desk
- Luggage storage
- Number of buildings/towers - 4
In the room
- Coffee/tea maker
- Iron/ironing board (on request)
- Hair dryer (on request)
- Flat-screen TV
- Free WiFi
Top London hotels
Recently reviewed hotels in London
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"Large room with private bath, perfect for a family (or small group that likes each other) that wants to visit London. Next to a busy street, but that's what London is all about.…"Jun 22, 2015
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Nearby hotels
Kensington Court Hotel Notting Hill, London's small print
Also known as
- Kensington Court Hotel Notting Hill
- Kensington Court Hotel Notting Hill London
- Kensington Court Notting Hill
- Kensington Court Notting Hill Hotel
- Kensington Court Notting Hill London
- Kensington Hotel Notting Hill
- Kensington Notting Hill
- Notting Hill Kensington
This property requires a security deposit for incidentals that will be pre-authorized (not charged) on the credit card. To complete the reservation process and obtain additional information, please contact the office using the information on the reservation confirmation received after booking.
Only registered guests are allowed in the guestrooms.
This property does not have elevators.
The name on the credit card used at check-in to pay for incidentals must be the primary name on the guestroom reservation.
Mandatory fees
Breakage deposit: GBP 20 per stay
Optional extras
Parking is available nearby and costs GBP 24 per 24 hours
We have included all charges provided to us by this hotel. However, charges can vary, for example, based on length of stay or the room you book.


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All-weather rug for outdoor or indoor use.
EZ Clean – resists stains; is kid and pet friendly.
BRAND: Momeni
COLLECTION: Veranda
COLOR: Tangerine
MADE IN: China
CONSTRUCTION: Loop-Hooked
MATERIAL: 100% Synthetic (olefin/polypropylene)
THICKNESS: 1⁄2" (approx.)
WARRANTY: 1 year - manufacturer
Elegant traditional designs and subtle transitional motifs adorn these unique outdoor rugs. Hand-hooked of 100% polypropylene, Veranda is completely outdoor-friendly. With their EZ Care system, you just hose them down and they will offer years of outdoor enjoyment. UV protected and mildew resistant.
The digital images we display have the most accurate color possible. However, due to differences in...
Rugs Direct recommends you avoid using your vacuum's beater bar on any area rug. For complete information on area rug care and cleaning, please click here.
Rugs Direct in-home delivery is available for selected rugs in this collection.
Standard curb-side delivery of oversized rugs is free on most purchases from Rugs Direct.
However, special "white glove" delivery is available for selected rugs in this collection at additional cost. If you select this option, we will make an appointment to deliver your purchase at a time convenient for you and place the rug in...


text:
This content has been marked as final. Show 20 replies
15. Re: Custom Event Subscription48771 May 6, 2009 3:20 PM (in response to 190407)Are you certain that the block raising the event is being called? If the procedure raising the event is executing properly, and is not erroring, then I am at a loss as to where else to look. That is the last place that there could be a problem. Sorry I couldn't help you. If you figure it out, please let me know, as I am curious as to why it's not working.
I have created many custom events, and have gone through all the troubleshooting that has helped me in the past.
Good luck.
16. Re: Custom Event Subscription714433 Jul 28, 2009 4:13 PM (in response to 190407)Hi all.
I have the same problem. I have a trigger with wf_event.raise.
If I put a code into PL/SQL procedure works fine, but when I call from trigger "wf_event.raise" don't works.
Any help??
17. Re: Custom Event Subscription48771 Jul 28, 2009 4:17 PM (in response to 714433)You need to commiit after the wf_event.raise. Do not do that in the trigger, create a stored procedure with an autonomous transaction to raise the event and commit.
18. Re: Custom Event Subscription714433 Jul 29, 2009 4:59 AM (in response to 190407)Hi Shersh.
Thanks for your answer.
We have others two environments and the trigger works fine in they.
I can't see any diference.
Any idea???
19. Re: Custom Event Subscription48771 Jul 29, 2009 9:09 AM (in response to 714433)Did you try testing the subscription from the UI using the WF Admin Responsibility? How did you create the event and subscription? Did you create from the forms, or use a script/utility? If you did not create from the forms, make sure the event and subscription both have licensed_flag = 'Y'.
20. Re: Custom Event Subscription989231 Feb 5, 2013 8:11 AM (in response to 190407)Hi,
We had a similar problem when we were testing a few events. When the event was raised from workflow administratror the custom subscription was getting picked but when the same event was getting raised by pl/sql program it didn't get executed. After a lot of debug we found that the event key that was assumed to be a NUMBER in our sample subscription was a VARCHAR2 for few events and it was raising an exception which we didn't bother to capture in the custom subscription earlier. This was a silly mistake which took our lot of time.


text:
Scirra is the name of loosely organised group of volunteer software developers. Currently students working in their spare time, they write software because it is interesting, because it's a challenge, and because they want to increase what people expect from technology.
No members were found matching the criteria specified. We suggest you try the memberlist with no filter applied, to browse all member profiles.
Also why don't you join the community, its fast, easy and free and opens up plenty of great features for you to enjoy.


text:
Volunteer Application
Contact Details
PO Box #812143.
Wellesley, MA 02482-0014
Phone: (781) 235-1218
Stray Pets in Need: email@example.com
Upcoming Events
SPIN Meetings
LOCATION: 5 Overbrook Terrace, Natick, MA
START DATE: July 01st, 2015
TIME: 6:00pm
SPIN Direct Care Orientation
LOCATION: 5 Overbrook Terr. Natick, MA 01760
START DATE: July 15th, 2015
TIME: 6:00pm
Adopted Pets
We are enjoying our three new additions very much. Our house feels full and happy again since bring them home.
PO Box 812143
Wellesley, MA 02482
(781) 235-1218
We who serve Stray Pets in Need deeply appreciate your interest in volunteering
to help the animals. So that we may use your talents most wisely,
we request the following information:


text:
The final nail in the coffin.
This is VLC's biggest release yet.
European owners of Nokia's Windows RT tablet are in for a shock.
While the Surface 2 was a huge improvement for Microsoft, it now faces some stiff competition from one of Microsoft's closest allies.
The Surface 2 shows that Microsoft's initial bet on hybrid computing devices is beginning to pay off.
The rumors are true: Nokia's next Windows devices are all about big, beautiful screens.
Nokia will launch its first Windows RT tablet in September, and alleged photos of the device have already leaked via a Chinese tech site. But with RT's relative failure, why is Nokia trying its hand at an RT tablet?
Microsoft's plan to bring Windows to low-power ARM processors, Windows RT, has struggled from the start — and now high-level partners are losing faith.
Guest In tablets, Microsoft has lost its focus.
Microsoft still faces criticism for its Windows 8 operating system, but despite that, the software giant has sold more than 100 million licenses in its first six months of availability and important updates are on the way.
If you needed further evidence that tablets are more than a mere computing fad, just look at the latest numbers from Strategy Analytics.
Microsoft's Windows RT ambitions are off to a tepid start, and Samsung's had enough.
Microsoft sold a paltry number of Surface tablets in the last quarter — somewhere between 680,000 and 750,000 units — the research firm IHS iSuppli estimates.
Is there much consumer interest in Windows RT? Samsung isn't convinced.
"Mac OS is the best thing to happen to Windows RT," quip the trolls.
Undeterred by Microsoft's foray into the hardware arena, Nokia has a trick up its sleeve for its own Windows RT tablet.
Late to the party, Barnes & Noble is finally launching its Nook app for Windows 8.
Google is bringing its Search app to Windows RT, offering a users a much-needed alternative to Bing.
After Microsoft pitched the Surface as the perfect no-compromise device of the future, we found that it was anything but.
While I admire Apple's ability to define a new category of computing on the iOS platform, I've always felt the iPad experience to be a little lacking for my needs.
Microsoft's Surface will be shipping on Friday, and the early reviews are already in. The conclusion: It's an amazing piece of hardware, with desperately bad flaws in its operating system and app ecosystem.
Microsoft's Surface RT pricing is directly competitive with Apple's iPad.
According to Lenovo $300 will be the magic number for Windows RT tablets this fall.
Long battery life, small size, and multitouch screens: That's what Microsoft is promising its PC partners will soon deliver using a version of Windows 8 known as Windows RT. Today, the company delivered some specifics.
We've known since February that Microsoft was preparing new touch-enabled Office apps for Windows RT, but now it seems those tablet-focused Office apps won't have as many features as their desktop siblings.
It was only a matter of time before Microsoft's hardware partners began publicly criticizing the Surface tablet, Microsoft's first stab at building its own computing hardware.
Microsoft has confirmed that its slick-looking Surface tablet will launch on Oct. 26, the same day the company releases its Windows 8 operating system, as noted in its annual report to the SEC.
As if on cue, the rampant speculation about Microsoft's mysterious LA event today reached new heights over the weekend. Initially believed to be a tablet related announcement, more recent reports indicated that it could be the debut of a new device developed together with Barnes & Noble.
You'd think the first Microsoft tablet worth getting excited about would be coming from Nokia, but AllThingsD's Ina Fried is reporting that Microsoft is gearing up to launch its own iPad competitor at a Los Angeles event next week.
Were you holding out for an inexpensive Windows 8 tablet this holiday season? Probably not, but still, the option for cheap Windows 8 tablets appears to be gone now that a VR-Zone report says Microsoft will charge $85 for a license of Windows RT.
Microsoft has finally shed some light on the different versions of Windows 8 we'll see upon release, and thankfully, things are much simpler than past versions.