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The psychology of color
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Lion cubs give hugs.
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Little puppy wakes up.
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(BGM有)誕生日の特別企画として、2010年のビデオを使って作りました。As a special plan of Maru's birthday, I made "I am Maru 3." with videos of 2010. BGM: Purple Planet http://www.purple-pl...
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Cat photography for most people is a way to remember their pets' funny actions and playing around. But actually, cat pictures require more talent to move to the next level and become professional cat photographs.
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It doesn't have to be perfect to be beautiful
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I don't know where to begin when it comes to this one. Paul & Cassie planned their wedding for months and months and made it a very, very personal affair, but something tells me Cassie has been planning this wedding her whole life. Not only did they ...
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Individuals from Sudan are brought to the USA, cultural differences emerge.
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The Hobbit: Start of production, video from the set.
Watch as a patient police officer in Texas puts up with a friendly black cat clinging to him during a recent traffic stop. The ways of the affectionate felin...
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Anonymous @ on
pls give me research scope to do ph.d in this emerging field
On Monday July 6th, the nanoHUB will be intermittently unavailable due to scheduled maintenance. All tool sessions will be shut down early in the morning. Home directories and tools will be unavailable most of the day. We apologize for any inconvenience this may cause. close
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pls give me research scope to do ph.d in this emerging field
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I'm a postdoc doing research in high-energy particle physics. I also have a hobby interest in computer programming.
You can find me on Twitter, or check out my blog and personal website! Or you can email me at email@example.com.
However, please don't contact me through any other channel about something that should be handled through this site. If you do, I'll probably just tell you to post it here.
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Daemen College : Academics & Research : Division of Arts & Sciences : Visual & Performing Arts : Faculty : Full-Time Faculty : Laura Watts Sommer
Laura Watts Sommer earned her PhD from Temple University with her thesis Francesco Hayez and the Byronic Influence on Italian Romantic Painting (1999). Her articles include "Italian Romanticism and Academicism in an Unidentified Rest on the Flight into Egypt" for the Gazette des Beaux Arts (2002), "Shifting Identities: Politics, Poetry and Passion in Italian Nineteenth‐Century Portraiture" for Visual Resources (2007), and "Regionalism and Ruskin in the Ottocento Art Historical Narrative," a chapter in the 2012 anthology Ruskin, Venice, and Cultural Tourism. Her most recent project is a manuscript on the effect of Regionalism on the Ottocento art historical narrative. Professor Sommer teaches classes on theory and methodology in the Modern and Contemporary eras.
Daemen Professors & Students from several departments work at a public clinic to provide free medical care in Progreso Dos in the Dominican Republic during the semester break.
©2014 Daemen College : 4380 Main Street, Amherst, NY 14226 : 800.462.7652


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What Do You Think Of GMGM Now?
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Murray was not without his faults and failures - he was at the core of our teams playoff failures. But I like his methodical approach. Assigning Gaetan to shadow Bossy resulted in Gates potting 4 goals while limiting Bossy to 1. Pounding that giveaway prone young defensemen, and by not using his A1 goal scorer soley, to do so.
I always felt Murray was too obsessed with the dump and chase, but it obviously worked to the tune of being able to get a ton of shots in all those pivotal close playoff games where we choked. Like Bruce, he did steer us out of being a laughing stock.
* Murray faced some stiff competition in our division. Facing long established franchises, we just couldnt get over that offensive stonewalling in big playoff games, our Gs outplayed. He was helped by all the great D pickups by D. Poile. The D stands for defensemen, his specialty. He and Murray just couldn't get us over the hump offensively, or get A1 G play when it mattered most.
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John Milton: Life, Writing, ReputationPaperback British Academy Original Paperbacks
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- Publisher: Oxford University Press
- Format: Paperback | 224 pages
- Dimensions: 155mm x 231mm x 15mm | 340g
- Publication date: 7 October 2010
- Publication City/Country: Oxford
- Sales rank: 1,595,990
Product description
This volume offers a series of fresh explorations of the life, writing, and reputation of John Milton. The ten papers take us inside Milton's verse and prose, into the context of the events and the intellectual debates within which they were written, and into the later worlds within which his reputation evolved and fluctuated. Key topics discussed include: his political beliefs and career; the characteristics of his poetry - especially Paradise Lost; the literary influences upon his verse; his perception of women; and the ways he has been seen since his death. John Milton: Life, Writing, Reputation will be essential reading not only for scholars and students of literature, but for all those interested in Milton's work and reputation over the last four centuries.
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Even the most seasoned scholars should be grateful for a volume that lays out so clearly where the field stands as Milton enters his fifth century and where scholars can profitably go from here. Anthony Welch, Milton Quarterly
Table of contents
1. Milton: Literature and Life ; 2. Milton and the Classics ; 3. Milton on Knowing Good from Evil ; 4. Milton and the Poetry of the Fall ; 5. Milton and the Regicide ; 6. Milton's Christian Temper ; 7. Milton, Marriage, and the Politics of Gender ; 8. Milton and the Romantics ; 9. Milton in the Twentieth Century ; 10. Milton and his Biographers


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It's a well known fact that nothing is more delicious than pork meat especially if it's cooked in a 'German flair'! For pork lovers out there, here's your time to feast! Thanks to Bavarian House, a reasonable deal for a set meal both 2 and 4 pax is offered. The set consists of mouthwatering pork based dishes such as BBQ ribs, Frikadalen, Thuringer Bratwurst and more. Famous for their beer and pork, Bavarian House has never disappointed pork lovers. Pork lovers out there, hurry up and grab this appetizing deal right away and for those who haven't become a pork lover yet, it's never too late, Bavarian House will help you become one!
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An Excessive Heat Warning is in effect from 6/25/2015 9:00 AM to 6/27/2015 9:00 PM PDT for Mohave County. Learn more about how to stay safe in extreme heat and stay informed about future heat alerts.
Health News – Publications
ADHS offers a variety of free publications for you to read online or download. The publications available here are intended to highlight news and information in the field of public health.


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Even if you don't use Facebook, if any of your friends do and they have enabled FB to see contact information in their smartphones or computer address books, Facebook is keeping tabs on you anyway.
Remember, Facebook is a CIA asset.
Iran Deal Good for Regional Peace
5 minutes ago


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This engineer serves as UI designer, coder, report writer and support for the company?s internal applications and the reports which serve internal and external stakeholders. Our internal systems are critical to the day-to-day settlement and clearing operations of the exchange6/30/2015 New Orleans, LA Save This Job
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Alert Logic is a leading provider of on-demand compliance and security solutions for mid-sized organizations. Our on-demand platform utilizes software-as-a-service (SaaS) to enable our customers the easiest way to secure their networks and comply with policies and regulations.6/30/2015 Houston, TX Save This Job
Alert Logic is a leading provider of on-demand compliance and security solutions for mid-sized organizations. Our on-demand platform utilizes software-as-a-service (SaaS) to enable our customers the easiest way to secure their networks and comply with policies and regulations.6/30/2015 Houston, TX Save This Job
Alert Logic is a leading provider of on-demand compliance and security solutions for mid-sized organizations. Our on-demand platform utilizes software-as-a-service (SaaS) to enable our customers the easiest way to secure their networks and comply with policies and regulations.6/30/2015 Houston, TX Save This Job
Team description:Each month, AppNexus transacts billions of ad impressions, which in turn powers the economic engine of the Internet. The Business Systems team is responsible for engineering systems that allow AppNexus to operate its business smoothly and effectively. We make5/24/2015 New York, NY Save This Job
Engineering Campbell, CA, United States Senior Software Engineer ? Mobile Platform Are you interested in building the future of customer service interactions? Are you excited by the opportunities that mobile has opened up for simplifying customer experience? We are looking5/11/2015 Campbell, CA Save This Job
The 247 Service Delivery Applications team builds world-class mobile, web, and speech recognition applications, as well as framework components. Our mission is to improve the customer service experience for millions of consumers who interact with major enterprises around the6/10/2015 Campbell, CA Save This Job
This role of a Software Engineer, Devices is an exceptional opportunity to work on the client side of the platform including mobile devices (such as iPhones/iPads, Android phones and tablets, Windows phones and tablets), game consoles (Xbox, PS3), and set top boxes and TVs in a5/24/2015 San Mateo, CA Save This Job
Location: Santa Barbara, CAType: Full TimeMin. Experience: Experienced InTouch Health is the leader in robotic telehealth solutions for healthcare. We are looking for an eager, self-starting Staff Software Engineer to join our team! This position will collaborate with a team of5/23/2015 Santa Barbara, CA Save This Job
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Today?s web and cloud applications are complex distributed systems, sometimes spanning hundreds of servers. The companies that design and manage these applications wage a daily war against outages and performance problems. You?ve probably experienced this for yourself, for4/14/2015 Morrisville, NC Save This Job
Overview: This position is responsible for managing day to day QA activities on several product teams. Day to day activity includes QA operations on multiple teams, test planning, test strategy work, and reviews of major QA deliverables. Additionally, this role will be5/16/2015 Pittsburgh, PA Save This Job
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NYC Type: Full Time Min. Experience: Experienced At Dataminr, we are building web apps with the latest browser technology in CSS3, HTML5, Backbone.js, node.js and many others. We are looking for engineers with strong experience in the development of web apps to help us build5/13/2015 New York, NY Save This Job
LEAD SOFTWARE DEVELOPMENT ENGINEER IN TEST (SDET)Â The mission of the Quality Assurance team is to create technology frameworks, implement quality best practices processes, and develop tools to ensure we deliver optimal end user experience for our products. At DemandMedia, we7/1/2015 Santa Monica, CA Save This Job
Betaworks We?re working on a brand new project at betaworks, and we?re looking for an experienced front end engineer to join the team. This is a super early stage product, so your job will by definition include a bunch of different projects and challenges, and you'll be very5/25/2015 New York, NY Save This Job
Summary: BATS, the second-largest U.S. equities market center, is actively seeking a top-notch software engineer with experience in dynamic website design and development. Location: Lenexa, Kan. What We Do We produce tools to run some of the world?s largest stock and options5/2/2015 Lenexa, KS Save This Job
About Us: ? Glooko helps people with diabetes, as well as their doctors and care team, manage the disease more easily and cost effectively. ? Launched in 2010, Glooko is funded and managed by visionary technologists and leaders in healthcare ? Achieved clearance by the FDA to5/26/2015 Palo Alto, CA Save This Job
SoundHound is creating and productizing transformative technologies that improve life. Join us. After amassing a global user community over 200 million strong, a large portfolio of core technologies and award winning products, we decided that is not enough. So, we put together a5/25/2015 San Francisco, CA Save This Job


text:
Just wondering, we are trying to get a layout to suit our site, but everything appears to either left aligned or not set to use 100% of the page width.
Have tried the obvious, editing CSS setting in various places but unable to locate a fix for this, however we have found a code snippet that we cant locate anywhere.
"If we set grid number to 3, the setting states width="26%" or if set to 4, the setting changes width="18%"
Obviously these are the incorrect percentages as they should be nearer grid of 3="33%" and grid of 4="25%". But we cant figure out where this snippet is being hooked from in order to reset it or if we are simply looking to deep into the code.
The snippet we have found looks like this:
.product_grid_display .product_grid_item {
width: 26%;
.product_grid_display .product_grid_item {
width: 18%;
Any input would be very gratefully received as we are having a lot of "white space because of this setting. :-)
Many thanks in advance.


text:
The exhibit will prominently display the entire gamut of LED Global Corp's lights, including their interior collection of pars, MR16s, down and spot lights, their flat bay collection of high bays, low bays and wall packs as well as their flat street and tube collection.
The exhibit runs from the 1st of October through the 3rd of October, 2012.
About LED Global Corp
LED Global Corp is a global manufacturing and distribution company of state of the art LED lighting technologies spanning a portfolio of interior and exterior LED lights utilizing Nichia diodes with a lifespan of 100,000 hours. The company is headquartered in Los Angeles, California and has operations in Europe, Middle East and Asia.
LED Global Corp has deployed LED lighting solutions in the United States, Asia and Europe. Our LED lighting products are manufactured in Taiwan.
For more information please visit www.ledglobalcorp.com.
Press contact:
Valerie Krueger
Public Relations


text: Feb 23, 2013 19:45 Travel agents say no effect yet from Triumph Travel agents say no effect yet from Triumph In this aerial photo, the cruise ship Carnival Triumph is towed Thursday into Mobile Bay, Ala. The ship with more than 4,200 passengers and crew members has been idled for nearly a week in the Gulf of Mexico following an engine room fire. (AP Photo/Gerald Herbert) This Sunday photo, provided by Kalin Hill, of Houston, shows passengers with makeshift tents on the the deck of the Carnival Triumph cruise ship at sea in the Gulf of Mexico. (AP Photo/Kalin Hill) The cruise ship Carnival Triumph is towed towards Mobile Bay near Dauphin Island, Ala., on Thursday. (AP Photo/Dave Martin) BETH J. HARPAZ| AP Travel Editor Feb. 23, 2013 Comments NEW YORK (AP) — Some travel agents on Thursday said cruise prices and bookings have not been affected by reports about a disabled Carnival ship, but others in the industry say it's too early to tell. The Triumph was being towed to Mobile, Ala., Thursday amid reports of filthy conditions onboard after the ship lost power at sea due to a fire. "As of now, cruise bookings throughout Travel Leaders Group have not been impacted and we are not seeing cancellations," said Kathy Gerhardt, spokeswoman for Travel Leaders, a network of independently owned and operated travel agencies in the U.S. Gerhardt noted that the cruise industry is in the middle of "wave season," the six-week period each winter when cruise vacations are promoted for spring and summer travel with deals and advertising, "and our bookings to date have been very strong and robust compared to one year ago." Heidi Allison-Shane, a spokeswoman for CruiseCompete.com, said the website has "seen above-average quote requests as well as bookings over the last five days with very few cancellations. Prices remain the same." But others sounded a note of caution. "Initial reports from travel agents indicates no impact yet, but there are clear concerns that after the ship returns to Mobile and videos come out, that may turn the tide," said Michael Driscoll, editor of the industry newsletter Cruise Week. "No single cruise ship problem has been so vividly reported over a drawn-out period of time, other than Concordia," the ship that capsized off the coast of Italy in January 2012, killing 32 people. Driscoll said Carnival canceled a one-day sale this week and will be hit harder than other cruise lines by the Triumph story. Carnival owns the company that operated the Concordia, and a third Carnival ship, the Splendor, lost power at sea in 2010 and was towed back to port under similar conditions to those on the Triumph. Driscoll added that "many cruise sellers expect a harsh round of media coverage to spike Friday and over the weekend, as firsthand reports and visuals get reported in national media and the hometowns of people once they return from the cruise. Logically, that may cause a downward blip in bookings, but it is expected to pass quickly once the situation dies down." But Carolyn Spencer Brown, editor of CruiseCritic.com, said that while "travel agents are going to say this is not going to have any impact," she believes "it's too soon to tell." She added that consumers are right to be concerned: "This is a cruise line problem. This is not an act of God or nature." The Costa Concordia's effect on the cruise industry unfolded over a period of months in 2012, as revenues grew at a slower rate than had been expected. A November 2012 report by PhoCusWright, the global travel market research company, said the cruise industry's growth rate fell to 4 percent in 2012, down from 7 percent in 2011. In addition to the Concordia, the industry was hurt by the U.S. recession and European financial crisis. But on Thursday, PhoCusWright senior research director Douglas Quinby said the Triumph is in a "completely different category" from Concordia. "Events like the Triumph, while they're obviously very unpleasant ... they're rare and they do not tend to have a long shelf life within consumer consciousness, especially among the core cruiser set, those consumers that cruise frequently and love cruising." Diana Warschauer of Reno, Nev., was onboard the Carnival Splendor when it lost power at sea in 2010, and like other passengers, she was given a refund and a free future Carnival cruise, which she only recently booked. She said hearing about the Triumph "brought back everything. I didn't think I had PTSD until the other night." But even though the Triumph rekindled her memories of sickening smells and other discomforts on the Splendor, she's going ahead with the second cruise, partly because the ship leaves from New Orleans and she's already booked flights and hotels for her family of four. "If we were driving and all these other costs had not been incurred, I might not go," she said. Alyce S. Lee-Walker of Pinehurst, N.C., a veteran cruiser, said the Triumph won't stop her from booking a Mediterranean cruise this spring with her husband. "I don't think it is any more dangerous than walking across the street, flying in a plane, taking the subway," she said. Mark Schiffner, vice president and chief operating officer of Cruise Holidays International, said some of the travel company's "agents tell us they have received questions about this particular incident, but it has not scared away any clients. More Business stories Florida Boulevard office building sold Jul 2, 2015 18:14 Timothy Boone| email@example.com Website combines business services from several Louisiana agencies Jul 2, 2015 17:29 Timothy Boone| firstname.lastname@example.com Shell moves forward on Appomattox development; largest Gulf platform could produce massive oil daily Jul 2, 2015 13:13 BY TED GRIGGS| firstname.lastname@example.com Furniture Expo building sold near Cortana Mall Jul 2, 2015 17:29 Timothy Boone| email@example.com Houma-Thibodaux real estate firm merges with Latter & Blum Jul 1, 2015 13:59 Timothy Boone| email@example.com Six of nine Louisiana metro areas lose jobs, but state still up overall Jul 1, 2015 22:51 by bill lodge| email@example.com Pennington scientists' breakthrough discovery on body's fat use, diabetes goes against previous theories Jul 1, 2015 13:58 Advocate staff report Baton Rouge's First National Bankers Bank acquires Florida institution Jul 1, 2015 13:58 Timothy Boone| email@example.com Director says downtown Baton Rouge may not need new taxing districts Jun 30, 2015 12:02 Timothy Boone| firstname.lastname@example.com Louisiana businesses react to fraud-fighting computer chip credit cards, which mean transition costs, and a deadline Jun 29, 2015 14:41 Advocate staff and wire report Business Honors for June 28, 2015 Jun 27, 2015 22:34 Advocate story People in Business for June 28, 2015 Jun 27, 2015 22:34 Advocate story Business Briefs for June 28, 2015 Jun 27, 2015 22:34 Advocate story Gay marriage expected to boost wedding-related businesses Jun 29, 2015 10:29 Timothy Boone| email@example.com U.S. oil and natural gas rig count increases by 2; Louisiana gains 6 Jun 28, 2015 12:00 The Associated Press Weekly unemployment claims fall Louisiana Jun 28, 2015 12:00 The Associated Press Four area companies receive state Lantern Awards Jun 28, 2015 12:00 by bill lodge| email@example.com MidSouth Bank names Troy Cloutier president Jul 1, 2015 22:51 Advocate staff report Entrepreneur event coincides with Essence Festival Jun 29, 2015 06:43 Advocate staff report Copa Airlines begins service between New Orleans and Panama Jun 27, 2015 22:34 Advocate staff and wire report Harvey Gulf Marine acquiring shipbuilders in New Orleans, Gulfport Jun 27, 2015 22:34 Advocate staff report Walk-On's picks Texas for its first location outside Louisiana, but you probably won't guess which city Jun 27, 2015 22:34 Timothy Boone| email@example.com What the Supreme Court's Obamacare ruling means for Louisiana residents Jun 27, 2015 22:34 BY TED GRIGGS| firstname.lastname@example.com C.J. Guillory -- 'the backbone of Gerry Lane' -- celebrates 100th birthday in style Jun 27, 2015 22:34 by bill lodge| email@example.com Retail spending in East Baton Rouge Parish drops slightly in April Jun 27, 2015 22:34 Timothy Boone| firstname.lastname@example.com Monsanto announces plan for $1 billion Luling plant expansion Jun 27, 2015 22:34 Advocate staff and wire report Jindal, GOP hopefuls caught on horns of Obamacare dilemma Jun 27, 2015 22:34 Advocate staff report Exterran closing Broussard oil, gas equipment plant, laying off 76 Jun 28, 2015 19:33 Bill Lodge| email@example.com Saratoga stock removed from exchange Jun 27, 2015 22:34 BY TED GRIGGS| email@example.com Prairieville site sold for $1.4 million for Valero convenience store Jun 27, 2015 22:34 Timothy Boone| firstname.lastname@example.com Get The Advocate in your inbox Subscribe


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Findings for PPC in Gainesville
Gainesville Ranks Amongst Most Expensive Markets for Keywords in South Atlantic
SEP's ongoing research into PPC Campaigns has revealed that Gainesville was the 13rd most expensive city in South Atlantic in keyword bids in Google Adwords for the month of April 2015. Gainesville also ranks amongst the most expensive cities in Florida, ranking 7th. In the study, North Charleston ranked as the most expensive city in South Atlantic for keywords, while Norfolk ranked as the least expensive city.
Google Adwords Keyword Prices 19% Higher in Gainesville than Jacksonville
SEP's research also tracks Google Adwords price changes and trends in cities across Canada and the U.S. Recent data shows that average prices in Gainesville are 19% higher than in Jacksonville. The average price of a click in Gainesville is $16.43.
This price differential is somewhat surprising however, as Jacksonville has a larger population than Gainesville.
To get a better sense of relative prices, we also compared prices with another city with a similar population, Elizabeth, and found the average price of a click in Gainesville was 214% higher than in Elizabeth.
The Price Rank shows how expensive keywords are in certain industry and segment relative to other cities in a country, region and province.


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Changes related to "Talk:Gravitation"
From Conservapedia
This is a list of changes made recently to pages linked from a specified page (or to members of a specified category).Pages on your watchlist are bold.
Legend: New! - new page, m - minor edit, b - bot edit.
5 July 2015
- (diff | hist) . . Physics; 13:43 . . (-43) . . SamHB (Talk | contribs) (Revert. Even if the linked page were meaningful (which it isn't), it isn't noteworthy enough to have its own link from the physics page.)


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|Reviews for The Will to Live
| I'maMePanda chapter 1 . 10/19/2013
This was very, very good. Xander's freak-out was so well done and realistic.
| Harm Marie chapter 1 . 4/16/2012
Loved this.
| emw314 chapter 1 . 10/17/2010
Just wanted to say that you write some beautifully poignant ficlets.
| The Ashes Fan chapter 1 . 8/9/2010
Lovely chapter. Very heartfelt and really shows the close bonds between the group but especially between Xander and Giles. Brava! Well done! :)
| medicgirl chapter 1 . 1/9/2010
This was amazing. The best kinds of families are the ones that are chosen. The downside is that they don't always realize that they are a family. Good for Xander for pointing it out.
| Wrinkled Fabric chapter 1 . 4/29/2009
OH man that was perfect. Brought tears to my eyes! I love the parental role Giles took on to all these kids and you showed that in a subtle, beautiful way. Hysterical Xander was heart-wrenching and so very realistic. Love it
| Z-L.B chapter 1 . 8/19/2008
That brought tears to my eyes. It is wonderful how he did become a father figure to basically fatherless kids. Buffy's dad was in LA, Willows parents where usually MIA and Xander's parents desperately needed to go to AA. I always wondered if Giles truly got how much he made a difference in their lives. ~ZLB
| Naithom chapter 1 . 8/17/2008
Magnificent. But you need to warn folks to bring Kleenex ahead of time.
| Tanokai chapter 1 . 7/26/2008
aww... cute! Glowing review! again.. o.o; I just love your stories!
| sapph89 chapter 1 . 5/27/2008
I cried. The entire time that Xander was telling Giles what he wanted from him I was crying. It took me about 20 min to get through the story because the words kept on blurring. Every time that I thought that I had it under control I would read the next sentence or paragraph and they would all blur together again. I loved it and I love the way that you portray Giles and Xander. Keep it up.
| Gremlinite chapter 1 . 9/9/2007
That was so sweet! I almost made myself sick thinking about Giles dying after reading that. That was a great look into the 'Scooby family' - especially Giles and Xander. Awesome.
| RagamuffinSundrop chapter 1 . 9/5/2007
Very sweet
| ivywatcher chapter 1 . 9/2/2007
Really, really sweet. I liked Xander in this; very true to character, a hint at the more mature version of himself that we see in later seasons. And I like that Giles does that for them without making a big deal out of it. Very him.
All in all, a lovely little piece that nicely shows Xander's heart and Giles' jaded mindset and the solid relationship between the two of them. Well done.
| Califaction chapter 1 . 8/20/2007
This fic is completely aww-worthy! Your Xander is very IC. I can picture him acting like this upon finding Giles' will. It's a little harder picturing Giles suggesting a camping trip, but he was getting to be a softy in season 3, wasn't he? Plus Xander had him all softened up with his anxiety about him dying. Xander's excitement, "Fishing? Really?" is typical of him, and infectious. You write good dialogue. This was a really wonderful, enjoyable fic!
Thanks for writing and posting!
| evil-mastermind666 chapter 1 . 8/15/2007
Hysterical Xander always makes me feel like the world is tilting in a very dangerous manner. It makes me break out in hives in worry. Good thing Giles isn't gonna die for a really really really long time right?


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Book Search
The Routledge Guidebook to Machiavelli's The Prince
Series: The Routledge Guides to the Great Books
Niccolò Machiavelli's The Prince is one of the most influential works in the history of political thought and the adjective Machiavellian is well-known and perhaps even over-used. So why does the meaning of the text continue to be debated to the present day? And how does a contemporary reader get...
To Be Published March 1st 2016 by Routledge
Hamilton Bailey's Physical Signs
Demonstrations of Physical Signs in Clinical Surgery, 19th Edition
It is approaching a century since the first edition of Demonstrations of Physical Signs in Clinical Surgery was first published, authored by the pioneering surgical teacher Hamilton Bailey. That it has survived is testimony to the continuing need for those learning surgery to be able to elicit...
Published January 5th 2015 by CRC Press
The Social Process of Lobbying
Cooperation or Collusion?
Series: Routledge Research in American Politics and Governance
Despite a wealth of theorizing and research about each concept, lobbying and norms still raise a number of interesting issues. Why do lobbyists and politicians engage in cooperative behavior? How does cooperative behavior in lobbying affect policy making? If democratic participation is good, why do...
Published December 18th 2014 by Routledge
Practicum and Internship
Textbook and Resource Guide for Counseling and Psychotherapy, 5th Edition
Completely revised and updated, the fifth edition of Practicum and Internship is an eminently practical resource that provides students and supervisors with thorough coverage of the theoretical and practical aspects of the practicum and internship process. New in this edition are: an...
Published September 17th 2014 by Routledge
'The Wars of Louis XVI,1667-1714', 'The Rise of the Great Powers, 1648-1815' and 'The Great Powers and the European States System, 1814-1914' - PACK
Published June 24th 2014 by Routledge
Suturing Principles and Techniques in Laboratory Animal Surgery
Manual and DVD
Suturing Principles and Techniques in Laboratory Animal Surgery: Manual and DVD introduces the basic principles of, and the materials and instruments required for, suturing and wound closure on animals in a research or veterinary facility. The manual provides descriptions and pictures portraying...
Published June 24th 2013 by CRC Press
Rail Human Factors
Supporting reliability, safety and cost reduction
The rail human factors/ergonomics community has grown quickly and extensively, and there is much increased recognition of the vital importance of ergonomics/human factors by rail infrastructure owners, rail operating companies, system developers, regulators and national and trans-national...
Published February 19th 2013 by CRC Press
Laboratory Mouse and Laboratory Rat Procedural Techniques
Manuals and DVDs
Despite the fact that the majority of research animals are rodents, the trainers at the Research Animal Resources Center at the University of Wisconsin-Madison found training material on the proper handling of mice and rats in biomedical research to be limited. So, they developed videos, narratives...
Published November 22nd 2010 by CRC Press
Laboratory Rat Procedural Techniques
Manual and DVD
This combination manual and DVD provides much-needed training on the proper handling of rats used in biomedical research. The DVD includes narrated video clips that demonstrate and describe each procedural technique. The manual contains handouts with color illustrations and descriptive text for...
Published November 19th 2010 by CRC Press
Laboratory Mouse Procedural Techniques
Manual and DVD
This combination manual and DVD provides much-needed training on the proper handling of mice used in biomedical research. The DVD includes narrated video clips that demonstrate and describe each procedural technique. The manual contains handouts with color illustrations and descriptive text for...
Published November 19th 2010 by CRC Press


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PRLog - May 18, 2012 - LANGPORT, U.K. -- Once again the onus is on the wonderful local producers and craftspeople that Somerset has to offer. The stall holders are all independent craftspeople, traders and include artisan producers such as Somerset Biltong, Wraxall Vineyard, Merricks Organic Farm, Feast Catering, Evans Butchers and many more. Several of the stall holders are award winners in their field and there will be samples and tasters available on the day.
Merricks Farm produce
A raffle of gifts, very kindly donated by the stall holders, will take place. The raffle proceeds are donated to a local charity. The happy recipients of the last raffle donation were the Langport & Huish Episcopi Youth Club who has put the money towards their camping trip.
Feedback from the last event was extremely positive with customers delighted to meet the producers. The March market exceeded all expectations on the day. The organisers are hoping for another glorious sunny morning and that, as before, the market will be supported by local residents and visitors alike. It is very much hoped that a market, on Langport's main square, will become a regular sight and plans are in place for regular markets in the future.
For more information or to take part in the markets, please contact Clare Aparicio on 07879 423117.


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There are currently two one games that are an exception to this rule and those are The Bulbasaur Race!, and Pokémon Trivia's DCC: Watch Out for Ghosts. Otherwise, there are plenty of chatting threads outside of Pokemon Trivia for both your Pokemon and non-Pokemon chatting needs!
Please refrain from posting in threads that haven't been posted in during the last 30 days.
If a thread is too old, make a new one. Constant revival [five times or more] will be infracted with 2 points that will last for two weeks.
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Have some common sense. Abide by all the normal PokeCommunity rules.
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Remember to have a good time and enjoy yourself! Thank you guys. :D


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New - Kamado Charcoal Ceramic Grill Smoker with Extras!
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Are you J Victor Garcia?Claim your profile
Publications (80)449.79 Total impact
- [Show abstract] [Hide abstract]
ABSTRACT: Since the onset of the HIV epidemic, there has been a shift from a deadly diagnosis to the management of a chronic disease. This shift is the result of the development of highly effective drugs that are able to suppress viral replication for years. The availability of these regimens has also shifted the neurocognitive pathology associated with infection from potentially devastating to a much milder phenotype. As the disease outcome has changed significantly with the availability of antiretroviral therapy, there is an opportunity to re-evaluate the currently available models to address the neurocognitive pathology seen in suppressed patients. In the following, we seek to summarize the current literature on humanized mouse models and their utility in understanding how HIV infection leads to changes in the central nervous system (CNS). Also, we identify some of the unanswered questions regarding HIV infection of the CNS as well as the opportunities and limitations of currently existing models to address those questions. Finally, our conclusions indicate that the earlier humanized models used to study HIV infection in the CNS provided an excellent foundation for the type of work currently being performed using novel humanized mouse models. We also indicate the potential of some humanized mouse models that have not been used as of this time for the analysis of HIV infection in the brain.Journal of NeuroVirology 11/2014; 21(3). DOI:10.1007/s13365-014-0299-6 · 3.32 Impact Factor
Article: Hypogammaglobulinemia in BLT Humanized Mice – An Animal Model of Primary Antibody Deficiency[Show abstract] [Hide abstract]
ABSTRACT: Primary antibody deficiencies present clinically as reduced or absent plasma antibodies without another identified disorder that could explain the low immunoglobulin levels. Bone marrow-liver-thymus (BLT) humanized mice also exhibit primary antibody deficiency or hypogammaglobulinemia. Comprehensive characterization of B cell development and differentiation in BLT mice revealed other key parallels with primary immunodeficiency patients. We found that B cell ontogeny was normal in the bone marrow of BLT mice but observed an absence of switched memory B cells in the periphery. PC-KLH immunizations led to the presence of switched memory B cells in immunized BLT mice although plasma cells producing PC- or KLH- specific IgG were not detected in tissues. Overall, we have identified the following parallels between the humoral immune systems of primary antibody deficiency patients and those in BLT mice that make this in vivo model a robust and translational experimental platform for gaining a greater understanding of this heterogeneous array of humoral immunodeficiency disorders in humans: (i) hypogammaglobulinemia; (ii) normal B cell ontogeny in bone marrow; and (iii) poor antigen-specific IgG response to immunization. Furthermore, the development of strategies to overcome these humoral immune aberrations in BLT mice may in turn provide insights into the pathogenesis of some primary antibody deficiency patients which could lead to novel clinical interventions for improved humoral immune function.PLoS ONE 10/2014; 9(10):e108663. DOI:10.1371/journal.pone.0108663 · 3.53 Impact Factor
Article: Preclinical Evaluation of TMC-278 LA, a Long-acting Formulation of Rilpivirine, Demonstrates Significant Protection from Vaginal HIV Infection.AIDS Research and Human Retroviruses 10/2014; 30 Suppl 1:A11-2. DOI:10.1089/aid.2014.5010.abstract · 2.46 Impact Factor
Article: The Role of Semen on Vaginal HIV-1 Transmission and on the Efficacy of Maraviroc as a Topically Applied Microbicide.AIDS Research and Human Retroviruses 10/2014; 30 Suppl 1:A50. DOI:10.1089/aid.2014.5087.abstract · 2.46 Impact Factor
Article: The use of BLT humanized mice to investigate the immune reconstitution of the gastrointestinal tract[Show abstract] [Hide abstract]
ABSTRACT: The gastrointestinal (GI) track represents an important battlefield where pathogens first try to gain entry into a host. It is also a universe where highly diverse and ever changing inhabitants co-exist in an exceptional equilibrium without parallel in any other organ system of the body. The gut as an organ has its own well-developed and fully functional immune organization that is similar and yet different in many important ways to the rest of the immune system. Both a compromised and an overactive immune system in the gut can have dire and severe consequences to human health. It has therefore been of great interest to develop animal models that recapitulate the key aspects of the human condition to better understand the interplay of the host immune system with its friends and its foes. However, reconstitution of the GI tract in humanized mice has been difficult and highly variable in different systems. A better molecular understanding of the development of the gut immune system in mice has provided critical cues that have been recently used to develop novel humanized mouse models that fully recapitulate the genesis and key functions of the gut immune system of humans. Of particular interest is the presence of human gut-associated lymphoid tissue (GALT) aggregates in the gut of NOD/SCID BLT humanized mice that demonstrate the faithful development of bona fide human plasma cells capable of migrating to the lamina propria and producing human IgA1 and IgA2.Journal of Immunological Methods 08/2014; DOI:10.1016/j.jim.2014.06.009 · 2.01 Impact Factor
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ABSTRACT: Antiretroviral therapy (ART) can reduce HIV levels in plasma to undetectable levels, but rather little is known about the effects of ART outside of the peripheral blood regarding persistent virus production in tissue reservoirs. Understanding the dynamics of ART-induced reductions in viral RNA (vRNA) levels throughout the body is important for the development of strategies to eradicate infectious HIV from patients. Essential to a successful eradication therapy is a component capable of killing persisting HIV infected cells during ART. Therefore, we determined the in vivo efficacy of a targeted cytotoxic therapy to kill infected cells that persist despite long-term ART. For this purpose, we first characterized the impact of ART on HIV RNA levels in multiple organs of bone marrow-liver-thymus (BLT) humanized mice and found that antiretroviral drug penetration and activity was sufficient to reduce, but not eliminate, HIV production in each tissue tested. For targeted cytotoxic killing of these persistent vRNA(+) cells, we treated BLT mice undergoing ART with an HIV-specific immunotoxin. We found that compared to ART alone, this agent profoundly depleted productively infected cells systemically. These results offer proof-of-concept that targeted cytotoxic therapies can be effective components of HIV eradication strategies.PLoS Pathogens 01/2014; 10(1):e1003872. DOI:10.1371/journal.ppat.1003872 · 8.06 Impact Factor
Article: HLA-B*57 Elite Suppressor and Chronic Progressor HIV-1 Isolates Replicate Vigorously and Cause CD4+ T cell Depletion in Humanized BLT Mice.[Show abstract] [Hide abstract]
ABSTRACT: Elite Controllers or Suppressors (ES) are HIV-1 infected patients who maintain undetectable viral loads without antiretroviral therapy. The mechanism of control remains unclear, but the HLA-B*57 allele is overrepresented in cohorts of these patients. However, many HLA-B*57 patients develop progressive disease and some studies have suggested that infection with defective viruses may be the cause of the lack of high levels of virus replication and disease progression in ES. We therefore performed a comprehensive comparative in vivo and in vitro characterization of viruses isolated from well-defined ES. For this purpose, we first performed full genome sequence analysis and fitness in vitro assays on replication-competent isolates from HLA-B*57 ES and HLA-B*57 chronic progressors (CPs). Under our experimental conditions we found that isolates from ES and CPs can replicate in vitro. However, since inherently these assays involve the use of unnaturally in vitro activated cells we also investigated the replication competence and pathogenic potential of these HIV isolates in vivo using humanized BLT mice. The results from these analyses demonstrate that virus isolates from ES are fully replication competent in vivo and can induce peripheral and systemic CD4 T cell depletion. These results provide the first direct in vivo evidence that viral fitness does not likely determine clinical outcome in HLA-B*57 patients and that elite suppressors can control replication-competent, fully pathogenic viruses. A better understanding of the immunological bases of viral suppression in ES will serve to inform novel approaches to preventive and therapeutic HIV vaccine design.Journal of Virology 01/2014; DOI:10.1128/JVI.03380-13 · 4.65 Impact Factor
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ABSTRACT: A nef gene is present in all primate lentiviral genomes and is important for high viral loads and progression to AIDS in human or experimental macaque hosts of HIV or SIV, respectively. In these hosts, infection of the thymus results in a decreased output of naive T cells that may contribute to the development of immunodeficiency. We have previously shown that HIV-1 subtype B Nef proteins can block human T-cell development. However, the underlying mechanism(s) and the conservation of this Nef function between different groups of HIV and SIV remained to be determined. We investigated whether reduction of thymic output is a conserved function of highly divergent lentiviral Nef proteins including those from both types of human immunodeficiency viruses (HIV-1 and HIV-2), their direct simian counterparts (SIVcpz, SIVgor and SIVsmm, respectively), and some additional SIV strains. We found that expression of most of these nef alleles in thymocyte progenitors impaired T-cell development and reduced thymic output. For HIV-1 Nef, binding to active p21 protein (Cdc42/Rac)-activated kinase (PAK2) was a major determinant of this function. In contrast, selective disruption of PAK2 binding did not eliminate the effect on T-cell development of SIVmac239 Nef, as was shown by expressing mutants in a newly discovered PAK2 activating structural motif (PASM) constituted by residues I117, H121, T218 and Y221, as well as previously described mutants. Rather, down-modulation of cell surface CD3 was sufficient for reduced thymic output by SIVmac Nef, while other functions of SIV Nefs contributed. Our results indicate that primate lentiviral Nef proteins impair development of thymocyte precursors into T cells in multiple ways. The interaction of HIV-1 Nef with active PAK2 by HIV-1 seem to be most detrimental, and downregulation of CD3 by HIV-2 and most SIV Nef proteins sufficient for reduced thymic output. Since the reduction of thymic output by Nef is a conserved property of divergent lentiviruses, it is likely to be relevant for peripheral T-cell depletion in poorly adapted primate lentiviral infections.Retrovirology 11/2013; 10(1):137. DOI:10.1186/1742-4690-10-137 · 4.77 Impact Factor
Article: E. E. Just award lecture.[Show abstract] [Hide abstract]
ABSTRACT: What started as a game of discovery for a child with a chemistry set has resulted in a lifetime of fulfillment and dedication to science. It is deeply rewarding to continuously let my curiosity ask questions for which there are no known answers. Equally rewarding has been the opportunity to mentor young students and postdoctorate graduates through their formative years in science.Molecular biology of the cell 11/2013; 24(21):3275-7. DOI:10.1091/mbc.E13-07-0407 · 5.98 Impact Factor
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ABSTRACT: The HIV-1 accessory protein, Nef, is decisive for progression to AIDS. In vitro characterization of the protein has described many Nef activities of unknown in vivo significance including CD4 downregulation and a number of activities that depend on Nef interacting with host SH3 domain proteins. Here, we use the BLT humanized mouse model of HIV-1 infection to assess their impact on viral replication and pathogenesis and the selection pressure to restore these activities using enforced in vivo evolution. We followed the evolution of HIV-1LAI (LAI) with a frame-shifted nef (LAINeffs) during infection of BLT mice. LAINeffs was rapidly replaced in blood by virus with short deletions in nef that restored the open reading frame (LAINeffs[increment]-1 and LAINeffs[increment]-13). Subsequently, LAINeffs[increment]-1 was often replaced by wild type LAI. Unexpectedly, LAINeffs[increment]-1 and LAINeffs[increment]-13 Nefs were specifically defective for CD4 downregulation activity. Viruses with these mutant nefs were used to infect BLT mice. LAINeffs[increment]-1 and LAINeffs[increment]-13 exhibited three-fold reduced viral replication (compared to LAI) and a 50% reduction of systemic CD4+ T cells (>90% for LAI) demonstrating the importance of CD4 downregulation. These results also demonstrate that functions other than CD4 downregulation enhanced viral replication and pathogenesis of LAINeffs[increment]-1 and LAINeffs[increment]-13 compared to LAINeffs. To gain insight into the nature of these activities, we constructed the double mutant P72A/P75A. Multiple Nef activities can be negated by mutating the SH3 domain binding site (P72Q73V74P75L76R77) to P72A/P75A and this mutation does not affect CD4 downregulation. Virus with nef mutated to P72A/P75A closely resembled the wild-type virus in vivo as viral replication and pathogenesis was not significantly altered. Unlike LAINeffs described above, the P72A/P75A mutation had a very weak tendency to revert to wild type sequence. The in vivo phenotype of Nef is significantly dependent on CD4 downregulation but minimally on the numerous Nef activities that require an intact SH3 domain binding motif. These results suggest that CD4 downregulation plus one or more unknown Nef activities contribute to enhanced viral replication and pathogenesis and are suitable targets for anti-HIV therapy. Enforced evolution studies in BLT mice will greatly facilitate identification of these critical activities.Retrovirology 10/2013; 10(1):125. DOI:10.1186/1742-4690-10-125 · 4.77 Impact Factor
Article: HIV-1 infection, response to treatment and establishment of viral latency in a novel humanized T cell-only mouse (TOM) model[Show abstract] [Hide abstract]
ABSTRACT: The major targets of HIV infection in humans are CD4+ T cells. CD4+ T cell depletion is a hallmark of AIDS. Previously, the SCID-hu thy/liv model was used to study the effect of HIV on thymopoeisis in vivo. However, these mice did not develop high levels of peripheral T cell reconstitution and required invasive surgery for infection and analysis. Here, we describe a novel variant of this model in which thy/liv implantation results in systemic reconstitution with human T cells in the absence of any other human hematopoietic lineages. NOD/SCID-hu thy/liv and NSG-hu thy/liv mice were created by implanting human fetal thymus and liver tissues under the kidney capsule of either NOD/SCID or NSG mice. In contrast to NOD/SCID-hu thy/liv mice that show little or no human cells in peripheral blood or tissues, substantial systemic human reconstitution occurs in NSG-hu thy/liv. These mice are exclusively reconstituted with human T cells (i.e. T-cell only mice or TOM). Despite substantial levels of human T cells no signs of graft-versus-host disease (GVHD) were noted in these mice over a period of 14 months. TOMs are readily infected after parenteral exposure to HIV-1. HIV replication is sustained in peripheral blood at high levels and results in modest reduction of CD4+ T cells. HIV-1 replication in TOM responds to daily administration of combination antiretroviral therapy (ART) resulting in strong suppression of virus replication as determined by undetectable viral load in plasma. Latently HIV infected resting CD4+ T cells can be isolated from suppressed mice that can be induced to express HIV ex-vivo upon activation demonstrating the establishment of latency in vivo. NSG-hu thy/liv mice are systemically reconstituted with human T cells. No other lymphoid lineages are present in these mice (i.e. monocytes/macrophages, B cells and DC are all absent). These T cell only mice do not develop GVHD, are susceptible to HIV-1 infection and can efficiently maintain virus replication. HIV infected TOM undergoing ART harbor latently infected, resting CD4+ T cells.Retrovirology 10/2013; 10(1):121. DOI:10.1186/1742-4690-10-121 · 4.77 Impact Factor
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ABSTRACT: Abnormal gut-associated lymphoid tissue (GALT) in humans is associated with infectious and autoimmune diseases, which cause dysfunction of the gastrointestinal (GI) tract immune system. To aid in investigating GALT pathologies in vivo, we bioengineered a human-mouse chimeric model characterized by the development of human GALT structures originating in mouse cryptopatches. This observation expands our mechanistic understanding of the role of cryptopatches in human GALT genesis and emphasizes the evolutionary conservation of this developmental process. Immunoglobulin class switching to IgA occurs in these GALT structures, leading to numerous human IgA-producing plasma cells throughout the intestinal lamina propria. CD4(+) T cell depletion within GALT structures results from HIV infection, as it does in humans. This human-mouse chimeric model represents the most comprehensive experimental platform currently available for the study and for the preclinical testing of therapeutics designed to repair disease-damaged GALT.Cell Reports 06/2013; 3(6). DOI:10.1016/j.celrep.2013.05.037 · 7.21 Impact Factor
Article: Rectal Transmission of Transmitted/Founder HIV-1 Is Efficiently Prevented by Topical 1% Tenofovir in BLT Humanized Mice[Show abstract] [Hide abstract]
ABSTRACT: Rectal microbicides are being developed to prevent new HIV infections in both men and women. We focused our in vivo preclinical efficacy study on rectally-applied tenofovir. BLT humanized mice (n = 43) were rectally inoculated with either the primary isolate HIV-1JRCSF or the MSM-derived transmitted/founder (T/F) virus HIV-1THRO within 30 minutes following treatment with topical 1% tenofovir or vehicle. Under our experimental conditions, in the absence of drug treatment we observed 50% and 60% rectal transmission by HIV-1JRCSF and HIV-1THRO, respectively. Topical tenofovir reduced rectal transmission to 8% (1/12; log rank p = 0.03) for HIV-1JRCSF and 0% (0/6; log rank p = 0.02) for HIV-1THRO. This is the first demonstration that any human T/F HIV-1 rectally infects humanized mice and that transmission of the T/F virus can be efficiently blocked by rectally applied 1% tenofovir. These results obtained in BLT mice, along with recent ex vivo, Phase 1 trial and non-human primate reports, provide a critically important step forward in the development of tenofovir-based rectal microbicides.PLoS ONE 03/2013; 8(3):e60024. DOI:10.1371/journal.pone.0060024 · 3.53 Impact Factor
Article: A Cluster of Virus-encoded microRNAs Accelerates Acute Systemic Epstein-Barr Virus Infection but does not Significantly Enhance Virus-induced Oncogenesis In Vivo.[Show abstract] [Hide abstract]
ABSTRACT: Over 90% of the adult human population is chronically infected with the Epstein-Barr virus (EBV), an oncogenic herpesvirus. EBV primarily infects naïve human B cells and persists latently in memory B cells. Most individuals experience an asymptomatic infection that is effectively controlled by the adaptive immune response. However, EBV-associated lymphomas can develop in immune compromised individuals. These tumors typically express all nine EBV latent proteins (latency III). Latency III is also associated with the expression of three precursor microRNAs (miRNAs) located within the EBV BHRF1 gene locus. The role of these BHRF1 miRNAs was unclear until recent in vitro studies demonstrated that they cooperate to enhance virus-induced B cell transformation and decrease the antigenic load of virus infected cells, indicating that the BHRF1 miRNA cluster may serve as a novel therapeutic target for the treatment of latency III EBV-associated malignancies. However, to date, it is not known if BHRF1 miRNAs enhance virus-induced oncogenesis and/or immune evasion of EBV in vivo. To understand the in vivo contribution of the BHRF1 miRNA cluster to EBV infection and EBV-associated tumorigenesis, we monitored EBV infection and assessed tumor formation in humanized mice exposed to wild-type virus and a viral mutant (Δ123) that lacks all three BHRF1 miRNAs. Our results demonstrate that while the BHRF1 miRNAs facilitate the development of acute systemic EBV infection, they do not enhance the overall oncogenic potential of EBV in vivo.Journal of Virology 03/2013; DOI:10.1128/JVI.00281-13 · 4.65 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: Innate immune restriction factors represent important specialized barriers to zoonotic transmission of viruses. Significant consideration has been given to their possible use for therapeutic benefit. The apolipoprotein B mRNA editing enzyme catalytic polypeptide 3 (APOBEC3) family of cytidine deaminases are potent immune defense molecules capable of efficiently restricting endogenous retroelements as well as a broad range of viruses including Human Immunodeficiency virus (HIV), Hepatitis B virus (HBV), Human Papilloma virus (HPV), and Human T Cell Leukemia virus (HTLV). The best characterized members of this family are APOBEC3G (A3G) and APOBEC3F (A3F) and their restriction of HIV. HIV has evolved to counteract these powerful restriction factors by encoding an accessory gene designated viral infectivity factor (vif). Here we demonstrate that APOBEC3 efficiently restricts CCR5-tropic HIV in the absence of Vif. However, our results also show that CXCR4-tropic HIV can escape from APOBEC3 restriction and replicate in vivo independent of Vif. Molecular analysis identified thymocytes as cells with reduced A3G and A3F expression. Direct injection of vif-defective HIV into the thymus resulted in viral replication and dissemination detected by plasma viral load analysis; however, vif-defective viruses remained sensitive to APOBEC3 restriction as extensive G to A mutation was observed in proviral DNA recovered from other organs. Remarkably, HIV replication persisted despite the inability of HIV to develop resistance to APOBEC3 in the absence of Vif. Our results provide novel insight into a highly specific subset of cells that potentially circumvent the action of APOBEC3; however our results also demonstrate the massive inactivation of CCR5-tropic HIV in the absence of Vif.PLoS Pathogens 03/2013; 9(3):e1003242. DOI:10.1371/journal.ppat.1003242 · 8.06 Impact Factor
Article: Human Breast Milk and Antiretrovirals Dramatically Reduce Oral HIV-1 Transmission in BLT Humanized Mice[Show abstract] [Hide abstract]
ABSTRACT: Currently, over 15% of new HIV infections occur in children. Breastfeeding is a major contributor to HIV infections in infants. This represents a major paradox in the field because in vitro, breast milk has been shown to have a strong inhibitory effect on HIV infectivity. However, this inhibitory effect has never been demonstrated in vivo. Here, we address this important paradox using the first humanized mouse model of oral HIV transmission. We established that reconstitution of the oral cavity and upper gastrointestinal (GI) tract of humanized bone marrow/liver/thymus (BLT) mice with human leukocytes, including the human cell types important for mucosal HIV transmission (i.e. dendritic cells, macrophages and CD4⁺ T cells), renders them susceptible to oral transmission of cell-free and cell-associated HIV. Oral transmission of HIV resulted in systemic infection of lymphoid and non-lymphoid tissues that is characterized by the presence of HIV RNA in plasma and a gradual decline of CD4⁺ T cells in peripheral blood. Consistent with infection of the oral cavity, we observed virus shedding into saliva. We then evaluated the role of human breast milk on oral HIV transmission. Our in vivo results demonstrate that breast milk has a strong inhibitory effect on oral transmission of both cell-free and cell-associated HIV. Finally, we evaluated the effect of antiretrovirals on oral transmission of HIV. Our results show that systemic antiretrovirals administered prior to exposure can efficiently prevent oral HIV transmission in BLT mice.PLoS Pathogens 06/2012; 8(6):e1002732. DOI:10.1371/journal.ppat.1002732 · 8.06 Impact Factor
Article: Overlapping effector interfaces define the multiple functions of the HIV-1 Nef polyproline helix[Show abstract] [Hide abstract]
ABSTRACT: HIV-1 Nef is a multifunctional protein required for full pathogenicity of the virus. As Nef has no known enzymatic activity, it necessarily functions through protein-protein interaction interfaces. A critical Nef protein interaction interface is centered on its polyproline segment (P69VRPQVPLRP78) which contains the helical SH3 domain binding protein motif, PXXPXR. We hypothesized that any Nef-SH3 domain interactions would be lost upon mutation of the prolines or arginine of PXXPXR. Further, mutation of the non-motif "X" residues, (Q73, V74, and L75) would give altered patterns of inhibition for different Nef/SH3 domain protein interactions. We found that mutations of either of the prolines or the arginine of PXXPXR are defective for Nef-Hck binding, Nef/activated PAK2 complex formation and enhancement of virion infectivity (EVI). Mutation of the non-motif "X" residues (Q, V and L) gave similar patterns of inhibition for Nef/activated PAK2 complex formation and EVI which were distinct from the pattern for Hck binding. These results implicate an SH3 domain containing protein other than Hck for Nef/activated PAK2 complex formation and EVI. We have also mutated Nef residues at the N-and C-terminal ends of the polyproline segment to explore interactions outside of PXXPXR. We discovered a new locus GFP/F (G67, F68, P69 and F90) that is required for Nef/activated PAK2 complex formation and EVI.MHC Class I (MHCI) downregulation was only partially inhibited by mutating the PXXPXR motif residues, but was fully inhibited by mutating the C-terminal P78. Further, we observed that MHCI downregulation strictly requires G67 and F68. Our mutational analysis confirms the recently reported structure of the complex between Nef, AP-1 μ1 and the cytoplasmic tail of MHCI, but does not support involvement of an SH3 domain protein in MHCI downregulation. Nef has evolved to be dependent on interactions with multiple SH3 domain proteins. To the N- and C- terminal sides of the polyproline helix are multifunctional protein interaction sites. The polyproline segment is also adapted to downregulate MHCI with a non-canonical binding surface. Our results demonstrate that Nef polyproline helix is highly adapted to directly interact with multiple host cell proteins.Retrovirology 05/2012; 9:47. DOI:10.1186/1742-4690-9-47 · 4.77 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: The outcome of untreated HIV-1 infection is progression to AIDS and death in nearly all cases. Some important exceptions are the small number of patients infected with HIV-1 deleted for the accessory gene, nef. With these infections, disease progression is entirely suppressed or greatly delayed. Whether Nef is critical for high levels of replication or is directly cytotoxic remains controversial. The major problem in determining the role of Nef in HIV/AIDS has been the lack of tractable in vivo models where Nef's complex pathogenic phenotype can be recapitulated. Intravenous inoculation (3000 to 600,000 TCIU) of BLT humanized mice with HIV-1LAI reproducibly establishes a systemic infection. HIV-1LAI (LAI) replicates to high levels (peak viral load in blood 8,200,000 ± 1,800,000 copies of viral RNA/ml, range 3,600,000 to 20,400,000; n = 9) and exhaustively depletes CD4+ T cells in blood and tissues. CD4+CD8+ thymocytes were also efficiently depleted but CD4+CD8- thymocytes were partially resistant to cell killing by LAI. Infection with a nef-deleted LAI (LAINefdd) gave lower peak viral loads (1,220,000 ± 330,000, range 27,000 to 4,240,000; n = 17). For fourteen of seventeen LAINefdd-infected mice, there was little to no loss of either CD4+ T cells or thymocytes. Both LAI- and LAINefdd-infected mice had about 8% of total peripheral blood CD8+ T cells that were CD38+HLA-DR+ compared <1% for uninfected mice. Three exceptional LAINefdd-infected mice that lost CD4+ T cells received 600,000 TCIU. All three exhibited peak viral loads over 3,000,000 copies of LAINefdd RNA/ml. Over an extended time course, substantial systemic CD4+ T cell loss was observed for the three mice, but there was no loss of CD4+CD8+ or CD4+CD8- thymocytes. We conclude Nef is necessary for elevated viral replication and as a result indirectly contributes to CD4+ T cell killing. Further, Nef was not necessary for the activation of peripheral blood CD8+ T cells following infection. However, CD4+CD8+ thymocyte killing was dependent on Nef even in cases of elevated LAINefdd replication and T cell loss. This depletion of thymic T cell precursors may be a significant factor in the elevated pathogenicity of CXCR4 trophic HIV-1.Retrovirology 05/2012; 9:44. DOI:10.1186/1742-4690-9-44 · 4.77 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: Clinical trials testing microbicides and related biomedical interventions to block HIV transmissions have produced contradictory results and to date it is unclear why. Further elucidation of the molecular basis of mucosal HIV transmission and extensive pharmacokinetic and pharmacodynamic analyses are essential to implementing effective prevention strategies. Animal models are of critical importance to this effort and bone marrow-liver-thymus (BLT) humanized mice have recently emerged as a powerful small animal research platform for in vivo efficacy evaluation of mucosal and parenteral HIV-1 prevention interventions. The availability of this validated system for the pre-clinical evaluation of HIV-1 prevention approaches will accelerate the implementation of the best candidate interventions into clinical trials.Trends in Microbiology 04/2012; 20(6):268-74. DOI:10.1016/j.tim.2012.03.007 · 9.81 Impact Factor
- [Show abstract] [Hide abstract]
ABSTRACT: Here we demonstrate that a combination of tenofovir, emtricitabine, and raltegravir effectively suppresses peripheral and systemic HIV replication in humanized BLT mice. We also demonstrate that antiretroviral therapy (ART)-treated humanized BLT mice harbor latently infected resting human CD4+ T cells that can be induced ex vivo to produce HIV. We observed that the levels of infected resting human CD4+ T cells present in BLT mice are within the range of those observed circulating in patients undergoing suppressive ART. These results demonstrate the potential of humanized BLT mice as an attractive model for testing the in vivo efficacy of novel HIV eradication strategies.Journal of Virology 01/2012; 86(1):630-4. DOI:10.1128/JVI.06120-11 · 4.65 Impact Factor
Top Journals
- Journal of Virology (10)
- Human Gene Therapy (8)
- Virology (7)
- Retrovirology (7)
- Journal of Virology (6)
University of North Carolina at Chapel Hill
North Carolina, United States
- • Division of Infectious Diseases
- • Department of Medicine
- • Center for AIDS Research (CFAR)
University of Texas Southwestern Medical Center
Dallas, TX, United States
- • Department of Internal Medicine
- • Division of Infectious Diseases
University of Texas at DallasRichardson, Texas, United States
Harvard Medical SchoolBoston, Massachusetts, United States
St. Jude Children's Research HospitalMemphis, Tennessee, United States
The University of Tennessee Health Science CenterMemphis, Tennessee, United States
Fred Hutchinson Cancer Research CenterSeattle, Washington, United States


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For One Day Only: $1=$4, Thanks to Matching from BIG Donors
And thenas time passes, you perceive a change within. You begin to notice inefficiencies in the hierarchy, leaks in the system, mistakes or lacks in the production line. You are no longer so new to the job that you are silenced by the higher-ups, but you are also not so entrenched in the company that you can't see beyond the set infrastructure. You bring new blood and a fresh perspective that visualizes how and where change can be effective. You have ideas and foresight that can revolutionize the old, and the stale.
And soin your own way, you begin to make dents in the way things are done. At first, it is only on a small scale, but with growing courage, you begin to tackle issues company-wide. You suggest new projects and revolutionary proposals well beyond your jurisdiction. You want to inspire others to sail along with your dreams. What you lack in experience you are more than willing to make up in initiative, energy and exuberance.
And that's when you hit against the inertia. You encounter it at every step of the way. There is resistance. There are raised eyebrows. There are the naysayers and there are the know-it-alls. For every two steps of progress, someone is forcing you to regress one.
And soondespite your convictions, you once again discern a change within. The criticism is having its effect. The sparkle of determination in your eyes has dimmed. You begin to wonder why you are expending so much effort. For the first time you hear yourself saying, "This isn't my department" and "I can't change the world." You've settled down. You've begun to accept the status quo, the easy path − the one with less resistance. You stop tackling new undertakings. You've just entered into your complacent adulthood.
A baby enters this world. For his first many years, the child is discovering the wonder of his new world. He is awed by his environment and reverent of his elders who provide instruction, infrastructure and guidance. But, as time progresses, he becomes a teenager. That's when he realizes that things aren't functioning as perfectly as he originally believed. There are inconsistencies in the system, unfair hypocrisies, incongruities and distorted priorities. He wants change and what he knows he lacks in experience and wisdom, he's ready to make up with his stamina, convictions, enthusiasm and energy.
Then somewhere along the path to maturity, the teen encounters too many obstacles, too many naysayers, and too many people telling him to mind his own business and stop rebelling against what is. His inspiration becomes quashed; his initiative dies. To the outside eye, he may have "settled down" and outgrown his impractical idealism or youthful rebelliousness, but intuitively he knows he's lost a part of himself.
The Lubavitcher Rebbe saw the years of teens as ones full of an unparalleled idealism and strength that just needed to be channelled appropriately:
The period of adolescence is nestled between childhood and adulthood. Teenagers are overflowing with adrenaline and confidence, feeling: "I want to change the way the world works." Adults burdened by the pressures of everyday life, convince themselves that this is the way it is, but young people cannot tolerate such resignation.
Youth are rebellious. But rebellion is not a crime. It can be the healthiest thing for a human − an energy that inspires a person to not give up easily, to refuse to tolerate injustice, to not go along with an idea just because everyone else is thinking it.
Some of us retain our youthful drive our entire lives; others never experience it. No matter what our age, though, there is a "teenager" within each of us. In every situation that we find ourselves, we have the opportunity to find the positive aspects of our teenage perspective and harness its power. Bubbling within us is that youthful idealism–that ability to question the status quo, to fight for the underdog and to follow the dreams of our beliefs with undaunted courage and determination.
Let's not follow the naysayers. Or become one.
Chana Weisberg is the author of several books, including Divine Whispers-Stories that Speak to the Heart and Soul and Tending the Garden: The Unique Gifts of the Jewish Woman. She is an international inspirational lecturer on a wide array of topics and an editor at chabad.org. Visit Chana's blog "Let's Go for Coffee" at www.chabad.org/618216. She can be reached at email@example.com.
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Orlando was once a place where people came only to visit and vacation. Now it is home to a burgeoning Torah community, a place Jewish families can be proud to call home.
The smuggler's life has been changed forever. He is faced with a major criminal charge. He will probably be sent to prison.
"Thanks to a local philanthropist who shares our core mission, we now are able to connect more Jewish teens to Israel than ever before," said Todd Cohn, executive director of Southern NCSY.
In September 2013 he was appointed head rabbi of the IDF Central Command and is currently in charge of special projects for the IDF chief rabbinate.
Last month we outlined how a few years after Judah Touro's death a public movement was inaugurated by the citizens of New Orleans to erect a monument to his memory, and that opposition to this tribute came from a number of rabbis throughout the country who claimed that Judaism forbade the erection of any graven […]
Marceau suggested a dark reason for his wordless art: "The people who came back from the [concentration] camps were never able to talk about it…. My name is Mangel. I am Jewish. Perhaps that, unconsciously, contributed towards my choice of silence."
Anna Henriques, who hopes to one day head back to Jamaica, says, "Rabbi Raskin must be willing to respect what exists in Jamaica. The way to the future is to gently bring in the traditions of the past and at the same time embrace the idiosyncrasies of the Jamaican people."
The Silver Platter has it all: gorgeous photography, oodles of useful tips and, more importantly, incredible recipes that you will find yourself making again and again.
It may be that seeking to connect with the past is rooted in the impermanence and impersonality of modern life.
It is very hard to build a healthy marriage when you do not have good role models.
My best book is one that hasn't been published yet.
We tend to justify and idealize this division with pride attributing these tendencies as demonstrating a higher level of kedushah.
We're on one of those really long family road trips. The kind that parenting experts advise will imprint fond memories on your children's psyche. (How's that for guilt?) And the kind on which you never leave home without a bottle of Tylenol and your favorite cup of strongly caffeinated, black coffee.
We're on one of those really long family road trips. The kind that parenting experts advise will imprint fond memories on your children's psyche. (How's that for guilt?) And the kind on which you never leave home without a bottle of Tylenol and your favorite cup of strongly caffeinated, black coffee.
Last week, I bought a new brand of detergent.
It promises to remove all stains, even those stubborn, impossible to remove ones–or your money back. Guaranteed.
Last week, I bought a new brand of detergent.
It promises to remove all stains, even those stubborn, impossible to remove ones–or your money back. Guaranteed.
From the great synagogue in Tel Aviv to his performances in the role of Jean Valjean in the hit Broadway show Les Miserables, Dudu Fisher is an international star singer and cantor.
From the great synagogue in Tel Aviv to his performances in the role of Jean Valjean in the hit Broadway show Les Miserables, Dudu Fisher is an international star singer and cantor.
He looks at me with such a wistful expression in his clear blue eyes. His young shoulders are sagging and he appears to be carrying the world's burdens.
He looks at me with such a wistful expression in his clear blue eyes. His young shoulders are sagging and he appears to be carrying the world's burdens.
Printed from: http://www.jewishpress.com/sections/jewess-press/do-you-know-where-your-teenager-is-an-ode-to-all-teens/2008/09/24/
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Publications and Statistics
This page contains links to all the research and publications which have influenced and informed the development of the Quality Mark.
1. Quality Mark Annual Statistical Returns
Starting in 2010-11, we asked the Quality Mark holding institutions to submit annual statistics relating to their implementation of the QM scheme via an online system.
The first report (Quality Mark Universities' Annual Statistical Report 2010/11) summarises the statistics gathered from universities over the period 2007 - 2011.
The statistical return from universities was reviewed in 2012 and in 2013 a section for FE colleges was added.
This year's report (Quality Mark Annual Statistical Report, Academic Year 2013-2014, May 2015) is the final statistical return and includes comparisons with the two previous years for university data and with the previous year for college data.
Quality Mark Annual Statistical Report, Academic Year 2013-2014 (May 2015)
This final report is an analysis of the statistical data return submitted by 59 universities and 26 colleges.
Universities have submitted this dataset for three consecutive years. We have included in the report a number of comparisons and trends we have been able to identify over the past three years.
For colleges this is the second year of the return and the report draws comparisons where relevant and possible with the previous year's data.
Quality Mark Annual Statistical Report, Academic Year 2012-2013 (June 2014)
The information in this report has been collected from 62 Higher Education Institutions and 25 Further Education Colleges. This is the second year in which we have collected this information from Higher Education colleges in this format.
Further Education Colleges were invited to join the Quality Mark for the first time in 2012, following the pilot phase in 2009-2011, and this is the first time we have collected data from them. As a result we now have a more rounded snapshot of the experiences of care leavers in post-16 education.
Quality Mark Universities' Annual Statistical Report, 2011/12 (June 2013)
This report is an analyses of the data received this year, relating to the academic year 2011/12, and provides some interesting statistics which are useful indicators for where support can be further developed within institutions.
Quality Mark Universities' Annual Statistical Report 2010/11
Please note that this earlier report refers to the previous set of returns and is designed to show an overview of trends and patterns relating to care leaver students and activities at Quality Mark institutions.
2. Quality Mark research reports and publications
Assessing the Impact of the Buttle UK Quality Mark in Higher Education (June 2013)
This research, conducted by York Consulting LLP and funded by the KPMG Foundation,
The report reveals a dramatic improvement in awareness of the barriers that care leavers face in higher education as well as the provision now in place for them at HEIs since the introduction of the Quality Mark in 2006.
Support across Quality Mark institutions still varies and the report highlights the need for all to strive to achieve the top 'exemplary' level of assessment for their Quality Mark.
Please note: For the glossary, the equivalent to an Access Agreement in Wales is a Fee Plan.
The Buttle UK Quality Mark Model: Working with your institution to support Care Leavers in HE (April 2012)
Dr Louise Comerford Boyes, a Research Fellow from the University of Bradford completed a report in April 2012 on the impact of the Quality Mark for higher education.
The report highlights the benefits of the Quality Mark to HEIs in for example, building effective cross-sector collaboration, providing a basis for external evaluation, as well as a framework to develop strategic cross-departmental working.
A Quality Mark for Care Leavers; a Pilot Study for Further Education (July 2011)
The strong success of the Quality Mark led to many requests from the further education sector to consider a similar FE commitment to Care Leavers. Buttle UK acknowledged that many young people choose to stay in further education, sometimes to then go on to complete HE qualifications. In October 2009 therefore, Buttle UK embarked upon an 18-month development programme for an FE Quality Mark, with a pilot of 25 further education colleges drawn from different areas of the United Kingdom.
The pilot found an urgent need for a Quality Mark for the FE sector and as a result Buttle UK now offer a Quality Mark for FE institutions as of January 2012. The report summarises the development, implementation and impact of the programme, and draws conclusions and recommendations on the potential for a national roll out.
By Degrees: From Care to University (2005)
In 2004, Buttle UK completed the first ever research study of university students with a background in local authority care.
Read or download a Summary of the final 'By Degrees' Report's Findings, or read or download the final 'By Degrees' Report 'Going to university from care'.


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After Sunriseby Vikki Salmela
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I used Google Translate and it came out different from Neosquid's.
Does anyone think it sounds like they think we're excited to see characters didn't make it?
The Silence Bridge Guru
I'd want to see Megaman, and maybe more second stage evolutions for Pokeball Items. Assist trophy-wise, I have NO idea.


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Pershing Square Capital Management LP sold down its position in the combined pharmaceutical companies Actavis PLC and Allergan Inc. after the activist failed to push Allergan into the deal it wanted last year. Read More »
May 15, 2015
Pershing Square Capital Management LP sold down its position in the combined pharmaceutical companies Actavis PLC and Allergan Inc. after the activist failed to push Allergan into the deal it wanted last year. Read More »
In the month after activist William Ackman launched his fight against Herbalife Ltd. in 2012, the nutritional-products company made a new online purchase: the rights to billackmanlies.com. Read More »
An annual mergers-and-acquisition confab hosted by Tulane University's law school was buzzing for most of two days about the battle for Allergan Inc., which featured dueling cross-country lawsuits, a regulatory investigation, vitriolic attacks, and, eventually, a sale at a price most analysts and investors would have considered out of reach just a few months earlier.
It felt, some said, like old times. Read More »
The Sohn Investment Conference–long considered the Super Bowl of investor conferences–is turning 20 this year. That's two decades of high-profile hedge-fund managers unveiling market-moving short positions and massive new bullish bets. The lineup for this year's conference suggests more big news is in store. Read More »
Bill Ackman has a message for his "friends" who are considering buying shares of Salix Pharmaceuticals Ltd. at $170: Don't do it. Read More »
Hedge fund billionaire Bill Ackman went on offense on CNBC Friday morning after the Wall Street Journal reported late Thursday that federal prosecutors and the Federal Bureau of Investigation are probing potential market manipulation of Herbalife Ltd., a firm he's loudly proclaimed to be a pyramid scheme. Read More »
Warren Buffett's cachè in the investment world is like Michael Jordan is to the sports world: Rarely duplicated but often modeled.
Even some activist investors–akin to the jocks of Wall Street–are increasingly tying themselves to the famed Omaha native. Most prominent among them is Bill Ackman, who says the Oracle of Omaha has long served as a guide. Read More »
Sales are down at Herbalife. The company's guidance for 2015 is also down, and investors are selling out of Herbalife's stock Friday. Read More »
Three months after Actavis PLC announced its $66 billion acquisition of Botox-maker Allergan Inc., investors and analysts appear even more confident that the deal can drive the combined company's stock higher. Read More »
Although most of the opinion's 20-pages are devoted to showing why the D.C. decision bars Continental Western from litigating the Iowa case, the key passage in Tuesday's decision is contained in a footnote on the penultimate page. That's where Judge Pratt declares that he agrees with the legal reasoning of the D.C. court and says he would have ruled the same way:
"The Court notes that even if it were to reach the merits of Continental Western's claims, including the allegedly new claims, it would agree with the well-reasoned opinion of the very able Judge Lamberth in Perry Capital that the case must be dismissed," Judge Pratt wrote. Read More »
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