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Trimble Creek Lodge - Perfect Vacation Lodging for Families, Reunions & Groups of 4-16. Nestled in the Arkansas Ozarks. Summer getaway planners - reserve the entire resort and treat your family to a truly unique vacation that allows togetherness and getting away from the crowd in a pristine, natural setting on a 72 acre peninsula of beautiful Bull Shoals Lake. This Vacation Rental includes a Lodge plus three unique cottages, a bunkhouse, treehouse, and private boat and swim dock in a gorgeous, quiet cove. New for 2015: A 9 Hole Disc Golf Course situated near the Meadow and Barn at TCL!
Bull Shoals Lake is open and clean - perfect for family water activities like water-skiing, boating, tubing, wake boarding, fishing or just relaxing in the lake. Our private boat & swim dock is for our guests only and is located in a beautiful secluded cove. Solar powered and equipped with a fish cleaning station, we keep our dock well-maintained for your personal or rented boats. We keep the dock the way we know families who care about their boats like it! We also feed the fish so your little ones can enjoy fishing from the dock.
What makes Trimble Creek Lodge Unique? We, Michael & Lorilee (Lulu), are your Innkeepers during your stay at Trimble Creek Lodge, a resort with the privacy of a Vacation Home, the extras of a Country Inn and the adventure of a 72-acre Lakeside Peninsula. We offer one-of-a-kind vacations for families, reunions and groups of 6-15 in a unique set-up that includes: A spacious Lodge for gathering, three unique and charming, fully equipped cottages with many extras, plus private boat & swim dock, bunkhouse & treehouse.
We personalize for each of our groups. Homemade ice cream, double chocolate brownies, advice for fisherman and help celebrating a special occasion, birthday or anniversary are some of our unique offerings. Spend time together in the Lodge, on the dock or at one of the many porches throughout our resort. Then, spread out for sleeping in our 3 stand-alone cottages plus the bunkhouse. There are porches and patios around the Lodge & East Cottage and a great covered back porch at the Meadow House. We have gas and charcoal grills and provide most everything you need in our well equipped kitchens (waffle irons, crock pots, good knives, rolling pins, great pots, plenty of dishes although due to our wilderness setting, we do not have dishwashers), bathrooms and bedrooms (fluffy towels, high quality sheets we iron by hand for you, beautiful quilts, fresh flowers and American & English soaps). Bring your food and friends and you are set!
Bull Shoals has 1,000 acres of shoreline and the water is beautifully clean. Guest's love swimming off our swim dock and enjoying the seclusion of our beautiful natural location. This lake is so large, you can boat or water ski without getting close to other boats – one of the reasons families love this place.
Every vacation here includes at least one free family style food gift from our Trimble Creek Lodge favorites including our own hand churned ice cream, double chocolate brownies or fabulous crusty fresh baked rolls. Complementary cooking lessons with Lulu are part of the charm of this unique place.
What is your set up including sleeping arrangements?
An ideal space for all your group gatherings, card games, pool tournaments, and simply spending time with friends and family. The Lodge boasts fabulous lake views and is under the same roof as the East Cottage. This large, airy space houses the library, antique pool table, family-style dining tables, sofas, chairs, fireplace, hardwood floors, cherry paneling, ceiling fans and a twenty-two foot window wall with spectacular views of the lake and our Heart of the Ozarks hillsides. (We are at 900 feet elevation, the lake at 650). Attached to the East Cottage, and opening to back and front decks complete with grills, a sun deck, two sun-shading pergolas and outdoor furniture, the Lodge is a favorite with all our family reunions for lounging, refreshing, outdoor meals and catching up with those we love in our naturally mosquito-free location. The treehouse, foosball table (porch location) and nearby horseshoe pits provide additional outdoor fun for your group. The Hot Tub Room, a lovely redwood enclosed room with windows on three sides, houses our hot-tub-for-two and is also attached to the Lodge. About 1200 sf or a combined 2600 sf with the East Cottage. Free Wi-Fi.
East Cottage: Often the location for joint dinner preparations, the two and a half bedroom East Cottage is fully equipped with kitchen, lake view grill, bathroom, satellite TV, living room, sofas, chairs, air conditioning, linens, fireplace and two fridges for those extra vacation foods. Two queen-sized beds, two queen sofa sleepers, one futon, and one twin chair sleeper provide sleeping for 2-6 guests. Attaches directly to the Lodge. Free Wi-Fi. About 1400 sf.
Meadow House: A charming two-bedroom cottage style vacation home provides additional sleeping, kitchen and bathroom spaces for groups using the East Cottage and Lodge. A kitchen, family room, TV, bathroom, linens and air conditioning make the Meadow House perfect for families with younger children, grandparents, or those who enjoy a quiet getaway. A large covered porch provides lovely forest vistas where wild turkey regularly feed over rolling hills. Each bedroom sleeps two in queen beds and the family room also offers sleeping for teens or children on the two sofas. Sleeps 2-6. Free Wi-Fi About 900 sf
Bunkhouse: A separate sleeping space with a view of the forest. Located just west of the Lodge, it provides sleeping and adventure for 5-6 tweens or teens in one full size futon and two sets of bunk beds. Flashlights included! Guests sleeping in the Bunkhouse come into the Lodge or East Cottage to use a bathroom. Available Summer Only.
The Barn at Trimble Creek Lodge: A perfect additional 'away from the crowd' sleeping space for a couple. One bedroom with a queen bed, kitchen, bathroom, family room with full sized futon and lovely views of an oak tree dappled meadow, located between the Meadow House and the Lodge/East Cottage. Sleeps 2-4. Free Wi-Fi.
Bathrooms & showers: Trimble Creek Lodge offers a total of four guest bathrooms (one each in the Meadow House, East Cottage, Barn at TCL and Lodge). The Lodge bathroom has a large old-fashioned claw foot tub and a great assortment of bubble baths (not a shower though). We also have an outdoor shower located near the East Cottage with a privacy screen on three sides and the forest on the third. We sometimes host an 'outdoor shower competition' with fun-loving crowds and taking outdoor showers is part of the vacation fun for many of our summer groups.
To summarize – East Cottage: Green Room (1 queen), White Room (1 queen), Tile Room (1 double futon). Family Room (2 queen sofa sleepers and 1 twin chair sleeper). Meadow House: Blue Room (1 queen), Pink Room (1 queen), Family Room (1 large sofa & 1 love seat for teen or child sleeping); Bunkhouse: 1 full futon & 2 sets of bunkbeds; the Barn at Trimble Creek Lodge: 1 queen & 1 full futon; Lodge: everyone for fun, dinner or gathering 24/7 (no beds).
What else is nearby? The Buffalo National Scenic River is not far and also great for day trips and river floats. The nearby White River is famous for the great trout fishing and we are only an hour from the shows and shopping in Branson. The new Crystal Bridges Museum on American Art is about 1.5 hours from here in Bentonville, Just for fun you might want to visit the towns of Yellville and Flippin Arkansas for used books or grocery shopping. Some guests visit the town of Eureka Springs (1.5 hours west of here) for upscale boutique shopping and fabulous Victorian architecture set into the hills of Northwest Arkansas. The Ozark Folk Center, located under two hours away in Mountain View, works to preserve and share the heritage and way of life of Ozark mountain people. Visit their website, http://ozarkfolkcenter.com to learn what music, gardening, herb or pioneer craft workshops are available during your time here. Arkansas, named "The Natural State" has many beautiful outdoor areas for outdoor enthusiasts including lakes, rivers, trails and more. If you Google "Arkansas Trails" you will find many resources for outdoor activities and hiking in this region.
Our Guests are Saying:
'What a wonderful place for our family reunion! Thank you for a relaxing vacation at your 'nothing-more-to-be-desired place.' Looking forward to being with you again!' Mildred & Cleo MO
"Remarkably outstanding" only begins to scratch the surface of the feelings we all have for our Arkansas family vacation at your "best kept secret" lake country resort… WOW. Rich & Carla TX
'Y'all are amazing! You two go so way beyond what you have to do. You are an inspiration.' Jason & Joy AR
'Most comfy beds ever!' Ray & Lorrie MO
'What a bang up delight to find this place and two wonderful innkeepers to provide us with awesome experiences.' Paul & Barbara TX
'Thank you from all our hearts for making America feel like home! Sonia & Radu '- Romania
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Smoking allowed in designated outdoor locations. Absolutely no indoor smoking allowed....moreless
Lodge book library also features world music collection (CD's) of retired professional dancers & dance teacher....moreless
East Cottage, Lodge Common Area and 3 Porches/Decks have Spectacular Lake Views from 900 Feet Elevation. Over one mile of Clean Water and Natural Shoreline are Visible from our Location...moreless
A short walk or drive in our private, natural setting takes you to a Swim & Boat Dock for use only by our guests. Located in a Quiet Cove of Bull Shoals Lake...moreless
We are at 900 feet elevation and the Lake is at 650 allowing fabulous views from the Lodge and East Cottage...moreless
Stone fireplace in the Lodge common space which adjoins the East Cottage...moreless
High quality, ironed sheets. Plenty of kitchen towels & soaps. English soaps, lotions and shampoos and great towels in bathrooms. Extra swim towels also available....moreless
The Lodge space provides a separate airy room for gathering, games or dining on 3 dining room tables...moreless
Parking: Plenty of safe space on our 72 acres to park your boat trailer. Launch at nearby 125 Recreation Area. Keep your personal or rented boats in our private boat dock....moreless
Available in the Lodge, East Cottage, Meadow House & Barn. Many guests can also access WiFi in some outdoor locations. ...moreless
East Cottage has its own brick fireplace in the kitchen/family room area for cozy evenings....moreless
All getaways & vacations include at least one free dessert. Some Spring or Fall Getaways for smaller groups include one free Family Style dinner - Try our Shrimp Pasta, Grilled Pork Tenderloin, Slow Simmered Italian Beef or Smoked Turkey. Additional meals, desserts (home made ice cream, double chocolate brownies, gingerbread houses) and our own special home baked breads are available for purchase by some groups. Check with your on-site Innkeepers if the cook needs a break or if you need something from the store. Allow us to make desserts or set our tables with china & silver for your special occasions or holiday meals....moreless
Drip coffee makers, french press coffee pots and 1 coffee grinder...moreless
waffle irons, crock pot, good knives, rolling pins, great pots & pans, iron pans & dutch oven, plenty of dishes & silverware, multiple coffee pots, wine glasses, wine opener, toaster, gas & charcoal grills, corn pots, turkey roaster, spatulas, measuring spoons & cups, outdoor grill tools, first aid kits, flash lights. Need something else? Ask the Innkeepers who likely can get it for you....moreless
Guests tell us there is so much more here than at other places they have stayed!...moreless
Three kitchens, one each in the East Cottage, Barn at TCL & Meadow House...moreless
(3) One each in the East Cottage, Meadow House & Barn...moreless
Full size in East Cottage & Meadow House. Counter top oven in the Barn at TCL...moreless
(6) Natural Views from all Porches & Patios. You might see deer or turkey but you won't see other houses or people (other than your Innkeepers) while you are here. Plenty of Outdoor Seating for Meals and Celebrations. Lake View Patio with Grills Outside East Quarters, Lake View Deck Behind Lodge Window Wall, Lighted Porch West of Lodge, Covered & Lighted Porch with Fridge Near Horseshoe Pits & Treehouse, Covered Lodge Front Porch with Garden Views, Covered Back Porch with Outdoor Ceiling Fans & Meadow View Behind Meadow House....moreless
Disc Golf that is! New 9 hole Course on our property in 2015!...moreless
Located in a separate window-lined room, this two person hot tub is accessible from an outside door off the Lodge...moreless
|Weekend Night
|Monthly *
May 22 - Sep 7, 2015
5 night min stay
Changeover Day: Saturday
Rates are based on 8 guests; add $29 per night, per additional guest
My Standard Rate
2 night min stay
Rates are based on 4 guests; add $35 per night, per additional guest
Additional information about rental rates
We offer many extras for all our groups. VRBO "QUICK QUOTES" are sometimes incorrect for our property, which has many options. Call 870-436-3222 for your rate. Summers are scheduled 5-7 nights Sat-Sat & Summer Rates start at $2795 for 8 guests + fees for additional guests, $100 dock & cleaning fee for some groups-16 max.20% Discount for Summer Last Minute Getaways or Spring/Fall Weekend Rates!
Policy available by contacting owner when booking.
Paying through this free service protects your payment up to $10,000 against listing fraud. If you are not paying through HomeAway Payments always call the owner at the number listed on our website and never use instant money transfer services such as Western Union and MoneyGram.
Adding our Vacation Protection services can make sure your getaway goes smoothly, no matter what. We offer Cancellation Protection, Carefree Rental Guarantee, and Damage Protection so you can truly relax.
Car: Necessary
Summer reunions & groups reserve the entire resort. Trimble Creek Lodge is a cross between a Resort, a Vacation Home and a Country Inn, located on a lovely 72 acre peninsula of Bull Shoals Lake in a secluded, wilderness location. You will find plenty of extras like great beds, fresh flowers, ironed sheets and home-made desserts but you can cook for yourselves in our well equipped kitchens. One reason our place is great for reunions is our Lodge Common Space – an airy room with cathedral ceilings and plank oak floors overlooking the lake where you can gather day or night for games, conversations, or meals. This is a great lake for fishing, boating, waterskiing, tubing and other family water fun. The mile-long cove where our private dock is situated is not crowded and perfect for family water sports - you can always find a secluded cove to teach your kids to ski. New in 2015 is a 9 hole Disc Golf Course on Trimble Creek Lodge Property for our guests only! Within an hour's drive find Golf courses, Silver Dollar City, Branson, MO, White & Buffalo Rivers. Visit the town of Eureka Springs (1.5 hours) for upscale shopping and fabulous Victorian architecture. The Crystal Bridges Museum in Bentonville offers first-class American art in a fabulous natural setting just 90 miles from Trimble Creek Lodge.
Year Purchased: 2002
About the owner: Dear Families and Friends, We are honored by the many wonderful families who in the last 12 years have chosen Trimble Creek Lodge for their vacations or getaways. Over the years we created many memories during our family vacations with our children and extended family and friends. We hope you will do the same during your time here with us in Arkansas, the Natural State. We are fortunate our kids still return "to the Lake." In fact, our son still takes his summer vacation here, traveling from Japan and then Korea to Trimble Creek Lodge. (The picture of us is actually from his recent wedding in Tokyo!) As a family, we treasure the things that were most different from our daily lives – long (and competitive) card games, lazy afternoons on the lake, learning new ways to have fun in the water – tubing, skiing, wakeboarding, fishing and making up games of our own! Here at Trimble Creek Lodge we hope you enjoy leaving your normal lives behind and experiencing life in our unique, natural setting where summer days are distinctive, simple and relaxed. Enjoy fixing (and eating) family breakfasts or dinners when no one is rushed to get to work, soccer or school. Have the kids do the dishes (there are no dishwashers here except the natural kind - your kids!) Take the time to enjoy a quiet morning outdoors listening to the birds. Have a family story/discussion night and hear everyone's stories – Grandpa as well as young ones (Michael & Lorilee have a basket of story-starting questions). Please let us know any questions about Trimble Creek Lodge. Our hope is that your family and friends will build your own memories and vacation traditions here in one of the most pristine, natural environments you will find in all of the US.
Why the Owner Chose Bull Shoals:
We vacationed on Bull Shoals for years with our kids and realized there were no places quite like Trimble Creek Lodge - distinctive, yet simple and relaxed. Below is a list compiled by our guests of their favorite experiences here at Trimble Creek Lodge. Previous guests, please send us your favorites to add!
The Unique Benefits at this House:
Eat, sleep, ski, fish! Memorial Day – Mid September: Tube, Water-ski or wakeboard on the lake where the water is beautiful and clean. Game night in the Lodge – we have Cards, Poker chips, Apples to Apples, Guesstures, Scrabble, Monopoly, Dominoes, Jenga, Pool table (adults), Foosball (outdoor porch), Pictionary, Yahtzee, Uno and more - or bring your favorite games! Walk - Forget the treadmill while you're here, we have 72 acres for outdoor walking! (Remember the insect repellent and shoes for walking on rocky surfaces.) Walk to the dock – 6 minutes down, 10 minutes up a rocky Arkansas lane – about ¼ mile. Walk from the Meadow House to the Lodge, Lodge to Meadow House (1/4 mile), enjoy the views and scenery along the way. Or bring your bikes for riding between the Meadow House and Lodge (the hills surrounding the Lodge are rocky and a bit steep for biking although we have had some mountain bike enthusiast who disagree!) Swim (Memorial Day – Mid September) or relax off the dock – we have both sun and shade for swimmers and relaxers. Boat on the Lake – 1,000 miles of shoreline on the 10th largest lake in the US. Pack a picnic, find a nice cove, jump into the lake for a swim, play water volley ball or keep away. Nap or read in the boat. Enjoy this beautiful, clean and uncrowded lake. There are boat rentals nearby at the Buck Creek and 125 Marinas. If you are here over July 4, consider watching fireworks over the lake from a boat! Pack your family into boats and enjoy a mosquito-free fireworks display from the lake. Typically fireworks take place a few days before the 4th and also on the 4th at several locations on the lake (Buck Creek Marina (nearby), Sugarloaf Harbor (8 miles) and Bull Shoals Boat Dock (35 miles – we use our GPS on the way home in the dark!). Schedules for these are usually available in June.
Lodge / Interior Over looking Bull Shoals Lake houses 2 sofas & 3 dining tables.
Deck Behind Lodge with gorgeous lake view. East Cottage Pergola in Background.
Lodge partial view-comfy sofa and 1 of 3 dining or game tables for our groups.
Vintage Brunswick Pool Table for adult guests in Lodge. Library in background.
East Cottage Kitchen 2014. Note 2nd fridge in back hallway, new range & fridges.
East Cottage Half Room with Futon (between East Cottage & Lodge)
Boat, ski, tube and water fun on beautiful, clean & uncrowded Bull Shoals Lake!
Game table in Lodge. Partial view of Library & additional games in background
The Barn at TCL Kitchen with Dutch Door, adjoins family room.
Lodge exterior attached to windowed Hot Tub Room-Lake on opposite side of Lodge.
Fishing on Bull Shoals Lake made this fisherman smile!
Outside Guy's herbs and produce is sometimes shared with our special guests!
Gorgeous view from East Cottage & Lodge. Enjoy privacy on our 72 acre peninsula.
East Cottage Family Room partial view & one of two queen sofa sleepers
Private Boat and Swim Surrounded by National Forest at the end of a quiet cove.
East Cottage Green Room Queen Bed. Fluffy blankets, ironed sheets, quiet setting
Meadow House Kitchen. Each cottage has a unique look & vintage furniture.
All cottages have free WIFI & flatscreen TV's - Meadow House family room TV here
Porches and lit outdoor pergolas provide mosquito-free evening relaxation space.
The Barn at TCL bedroom- queen bed, ironed sheets, fluffy blankets, lovely quilt
Meadow House Pink Bedroom. All bedrooms have unique vintage dressers & queen beds
Tree House for the Young @ Heart!
Try our homemade pie for your free dessert-delicious with our homemade ice cream
The Cove where our dock is located is perfect for skiing and other water fun.
There have been visitors to this page since the counter was last reset in .
This listing was first published here in 2003.
Date last modified - Monday, July 06, 2015
(870) 436-3222 (Arkansas, USA)
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15. The method of
16. A printing method to fabricate a three-dimensional microfluidic structure, comprising:
printing a three-dimensional microfluidic structure made of light curable plastic;
curing the light curable plastic; and
removing uncured plastic.
17. The method of
18. The method of
19. A three-dimensional microfluidic valve network, comprising:
microfluidic flow tubes;
pressure chambers surrounding the microfluidic flow tubes; and
vias connecting the microfluidic flow tubes.
20. The network of
21. The network of
The present application claims the benefit of provisional application 60/634,668 for "Replication Molding of Three-Dimensional Valves" filed on Dec. 8, 2004 and provisional application 60/634,667 for "On-Chip Refrigerator and Heat Exchanger" filed on Dec. 8, 2004, both of which are incorporated herein by reference in their entirety. The present application is also related to U.S. application Ser. No. ______ (Attorney Docket No. 622900-0) for "Thermal Management Techniques, Apparatus and Methods for Use in Microfluidic Devices" and to U.S. application Ser. No. ______ (Attorney Docket No. 620351-4) for "Parylene Coated Microfluidic Components and Methods for Fabrication Thereof," filed on the same date of the present application, also incorporated herein by reference in their entirety.
This invention was made with U.S. Government support under contract No. R01 H6002644 awarded by the National Institute of Health. The U.S. Government has certain rights in this invention.
1. Field
The present disclosure relates to microfluidic devices, such as valves. In particular, it relates to methods and devices for replication of three-dimensional valves from printed wax molds or other types of rapid prototyping technologies, such as UV light curable polymers like PFPE.
2. Related Art
Recently, lithographic techniques have been successfully applied towards the miniaturization of fluidic elements, such as valves, pumps and limited three dimensional structures (see references 1-10). The integration of many devices on a single fluidic chip has enabled the development of powerful and flexible analysis systems with applications ranging from cell sorting to protein synthesis. Through replication molding and embossing from photolithographically patterned dies, inexpensive fluidic systems with pneumatic actuation have been developed, by several groups (see references 11-19). Hermetically sealed valves, pumps and flow channels can be formed in polydimethylsilicone (PDMS) and related compounds (RTV, etc.), and in multilayer soft lithography, two or more replication molded layers are aligned and subsequently bonded to create systems of pneumatic actuation channels controlling flow within a layer of flow channels.
For example, two-dimensional valves are disclosed in U.S. Pat. No. 6,929,030 to Unger et al., which is incorporated herein by reference in its entirety. The valves disclosed in Unger are called two-dimensional because they are an extrusion of a two-dimensional drawing. In particular, in Unger, a structure is obtained where a first two-dimensional layer is put on top of a second two-dimensional layer. The two layers are then bonded together. After that, one of the two layers is pressurized to push on the other. No fluid flows between these two layers.
As the geometry of the pneumatic valve determines the actuation pressure, it is possible to define pneumatic multiplexing geometries that permit the control of many valves on a microfluidic chip by a much smaller number of control valves off-chip (see reference 20). Unfortunately, the two-dimensional nature of the flow channel arrangement limits the interconnection of this kind of two-dimensional fluidic system. Moreover, multi-layer soft lithography requires the use of elastomeric materials that can bond well to each other to avoid delamination of the pneumatic film layer from the fluid flow layer.
According to a first aspect, a printing method to fabricate a three-dimensional microfluidic component is provided, comprising: forming a three-dimensional mold of the three-dimensional microfluidic component, the mold made of a first wax; providing a sacrificial material acting as a temporary support, the sacrificial material made of a second wax; dissolving the second wax; pouring a component material onto the mold; curing the poured component material; and melting away the first wax.
According to a second aspect, a printing method to fabricate a three-dimensional microfluidic structure is provided, comprising: printing a three-dimensional microfluidic structure made of light curable plastic; curing the light curable plastic; and removing uncured plastic.
According to a third aspect, a three-dimensional microfluidic valve network is provided, comprising: microfluidic flow tubes; pressure chambers surrounding the microfluidic flow tubes; and vias connecting the microfluidic flow tubes.
The structures disclosed in accordance with the present disclosure are truly three-dimensional, in the sense that both the control and fluid lines can be built in the same fabrication step, without need to bond them together. In a structure like the one shown in the present disclosure, separate control and fluid lines having different geometries can be built, together with vias or chambers encircling a channel.
Three-dimensional connections between fluidic layers offer more flexible design opportunities that are inaccessible with planar techniques.
The methods in accordance with the present disclosure allow to construct fluidic conduits that require structural supports only every few centimeters, as well as robust, tunable, three-dimensional valves which can control flow pressures of over 220 kiloPascals (33 psi).
The three-dimensional replication-molded microfluidic design is also insensitive to swelling caused by aggressive solvents.
Three-dimensional soft lithography offers many advantages over the more conventional multi-layer soft lithography, which is based on two-dimensional valve and pump definition. One key advantage of developing devices from three-dimensional replication molding is that it enables the use of a wide variety of elastomers and plastics that are more resistant to strong acids, bases and organic solvents. Moreover, the pressure in the flow channels can be increased and the actuation pressure of the pneumatic lines can be decreased by implementing designs that do not involve layers that may delaminate and can close the valve by applying pneumatic pressure from all sides.
An opportunity obtained from three-dimensional definition is the increase in inter-connectivity of the fluidic components and improvement in the flow channel integration in all three dimensions through the use of via holes that can jump over a fluidic layer or control layer with a commercially available wax molding system. A further opportunity is the ability to use fluorinated compounds. The first results obtained by applicants on this new kind of microfluidics indicate that denser integration with larger numbers of components and more complex fluidic multiplexing systems can be implemented through 3-D replication molding. Furthermore, the additional dimension allows the formation of larger diameter fluidic channels and enables fast flow and higher volume fluidic handling.
To solve the limitations of two-dimensional layered systems and to enable more flexible microfluidic plumbing topologies, the present application discloses a three-dimensional replication molding method that permits the construction of valves and pumps that are interconnected in all dimensions. To create three-dimensional replication dies, a commercial wax printing system can be used (e.g., Solidscape T66). The Solidscape T66 is a rapid protype machine (RPM) which can define features as small as 12.5 microns high by 115 microns wide. The person skilled in the art will understand that wax printing systems different from the Solidscape T66 machine or other rapid prototyping technologies (such as those producing a positive directly from light curable polymers like PFPE) can be used, so long as they allow microscale features to be obtained. Microfluidic components usually have a radius in the 10-500 microns range, preferably a 10-115 microns range, and most preferably a 10-100 microns range. The person skilled in the art will be able to select the adequate dimensions in order to allow the components to be integrated on a chip.
The combination of printed wax droplets with precise milling heads and stage positioning enables wax molds to be constructed with feature sizes comparable to those made by photolithography. The wax mold can be computer designed and printed directly onto a flat substrate without the need for any photolithography masks. The designer can fabricate three-dimensional microfluidic components interconnected with great flexibility.
According to a first embodiment, also shown in
According to a second embodiment, also shown in
During the printing process, also a sacrificial or support wax is provided, (S10) to temporarily support the desired, suspended structure during fabrication. The sacrificial or support wax is dissolved (S11) at the end of the fabrication process. If necessary, the fabricated build wax mold can be cured or dried (S12) by using, for example, air or an oven.
A subsequent step is that of pouring a polymer (S13) onto the mold. The polymer will form the "positive" of the structure, and can be a material such as PDMS (polydimethylsiloxane), PFPE (perfluoropolyether), SIFEL� (a fluorocarbon siloxane rubber precursor by Shin Etsu Chemical Co., Ltd) or parylene (a coating material). After pouring of the polymer, vacuum can be formed in the structure to better insert the polymer into the structure and to remove air out of the structure. The polymer is then cured (by heat, light etc.) and solidified (S14). The build wax mold ("negative") is then melted away (S15) to provide the desired microfluidic device geometry.
In accordance with the present disclosure, holes in the wax mold can be created for the introduction of steel pins to connect input or output tubing. The steel pins can be melted to the wax, glued or attached by slip fit.
According to a first embodiment, wax columns (i.e. negatives of a hole) can be formed in the build wax during the printing process of the negative (S16). The polymer will then be poured so that a portion of the wax column remains out of the polymer. In this way, when the build wax is melted away, holes will be formed.
According to a second embodiment, holes can be formed through punching (S17) in the final polymer chip.
According to a third embodiment, metal pins can be introduced or soldered into the build wax mold (S18), and later pouring the polymer over the build wax mold by leaving part of the pin above the top level of the polymer. After that, once the wax has been melted, the pin is pulled out. Typically, the wax mold will be constructed with areas specifically made to have the pins soldered in. The pins can be melted to the specifically made areas, glued or attached by slip fit.
A variation of this embodiment can also be provided, where the structure does not depend on glass in order to allow precise separation of the various components of the microfluidic chip. According to this embodiment, during formation of the mold, two additional build surfaces, a top surface and a bottom surface, are formed (S19). Reference can be made, for example, to surfaces 70 and 80 of
As mentioned above, one type of polymer that can be used is SIFEL�. SIFEL� is a liquid fluoroelastomer (fluorocarbon siloxane rubber precursor) that combines the characteristics of silicone and fluorine and softens into a rubbery texture when heated. Two types of SIFEL�—glue and non-glue—are commercially available. Punching of SIFEL� to form holes is not possible. Therefore, a possible way of forming holes in-this embodiment is that of forming them in the build wax mold, as described above. Alternatively, a metal pin of a smaller diameter of the pin to be later used for fluid introduction can be soldered. In order to do so, a solder point is designed and later formed in the build wax mold. SIFEL� is then poured from the top, in order to avoid its formation in the solder point. Presence of pin holes in an embodiment where glue-type SIFEL� is used is preferred, because glue-type SIFEL� will become attached to the glass support, thus precluding an exit way for the build wax upon dissolution. In this case, the build wax will come out through the pin holes. On the other hand, in case of non-glue-type SIFEL�, the build wax filled with SIFEL� can be detached from the substrate, and then taken out of the bottom of the structure.
Use of a PFPE polymer is similar to use of non-glue type SIFEL�. It should also be noted that both SIFEL� and PFPE usually cannot be bonded well to glass. In order to overcome this obstacle, a plastic clamp is machined, to allow for the pins or steel pins to protrude. Pressure is then applied to seal the glass to the polymer through the plastic clamp. The person skilled in the art will understand that the amount of pressure to be applied should be such that the polymer is sealed to the glass without crushing the microfluidic channels or valves formed in the structure.
According to a further embodiment, as also shown in
In order to provide the structure with pinholes, several choices can be made. According to a first choice, holes can be punched in the polymer chip—through both parylene and the polymer—after the build wax has been melted out, similarly to what shown in step S17 of
In accordance with a further embodiment, a method for parylene coating of two-dimensional microfluidic channels is disclosed, as also shown in
In a first step a substrate is coated with a thin layer of parylene for better adhesion for the next lithographic molds (S26). In order to provide a clean surface, the substrate surface is first dipped in 5% HF (fluoridic acid) and then treated using oxygen plasma (S27). The oxygen plasma can be generated in a Technic� parallel plate reactive ion etcher (MicroRIE) with a 170 W RF power, 20 sccm O2 flow rate, and a 30 s etching time. After plasma cleaning, an adhesion promoter (e.g., promoter A-174 from Specialty Coating Systems) can be applied (S28) to the surface to further enhance good adhesion between the parylene (see below)and the substrate. The substrate is then coated with a thin layer of parylene film of thickness between about 100 nm and about 2 micrometer. Coating promotes adhesion and provides passivation.
In a second step, a lithographic mold is formed (S25) in the same manner as described above and in
In a third step, the treated mold is conformally coated with a layer of parylene (S29).
In a fourth step, the mold is immersed in heated acetone (S30) to remove the sacrificial photoresist. The extremely thin parylene channels can be used as is. Such embodiment can be particularly useful for imaging what is inside the channels under an optical microscope or in an environmental SEM (scanning electron microscope), because the parylene is thin, so that a significant portion of the electron beam can penetrate the thin film and generate a scanning electron image.
Optionally, a thin layer of polymer (PDMS, PFPE or SIFEL�) is spinned over the parylene coated mold and cured (S31). The photoresist is then removed with heated acetone. In this way, a structurally robust channel is formed, still maintaining the structural properties of the polymer but protected from chemicals by the parylene.
Optionally, a control layer can be aligned and bonded with the polymer layer over the parylene coated channel in order to form a two-dimensional valve.
In accordance with the teachings of the present disclosure, the valve shown in
The maximum pressure range as well as the control over the valve actuating pressure compares very favorably with traditional planar valves constructed through multi-layer soft lithography. In comparison, the multi-layer soft lithography layers in accordance with the present disclosure delaminate at approximately 82 kPa (12 psi).
The applicants have designed a three-dimensional normally open valve geometry. The pneumatic 3-D valve of the present disclosure was also tested in solvents that are known to deteriorate PDMS channels. For example, the valve performance was evaluated when metering toluene, a material known to result in swelling of PDMS and deterioration and distortion of conventional PDMS fluidic systems. As the 3-D valve definition procedure in accordance with the present disclosure does not rely on multi-layer PDMS films that could delaminate, no leakage or deterioration could be observed in the 3-D valve after exposure to toluene. Although the tenability suffered due to swelling over time, the valve performance was not influenced.
As already mentioned above, the two surfaces 70 and 80 are taken out during the melting step. In addition, a further portion of the exposed top surface and the exposed bottom surface of the structure is cut, to allow separation of the microfluidic channels once the positive of the structure is obtained. Cutting of the further top and bottom portions will prevent undesired fluid contact among the various channels.
From this image, it is clear that large plumbing systems consisting of integrated arrays of microfluidic valves can be constructed by 3-D microvalve definition. In such a valve network, the density of fluidic elements can be significantly increased beyond what is available for more traditional 2-D microfluidic networks constructed from PDMS. In such 3-D fluidic chips, the smallest flow pressure line that can be defined by the lateral and vertical resolution of the wax printer is 115 microns wide by 12.5 microns high (although some difficult geometries require more material strength and must be made larger). These dimensions match well with geometries suitable for the definition of useful microfluidic "laboratory on a chip" applications.
Three-dimensional printing in accordance with the present disclosure eliminates the need for bonding the pneumatic control layer to the flow layer as both are formed in the same monolithic mold. This enables the use of elastomers that can be bonded only once or do not satisfy the adhesion requirements of multi-layer fabrication such as the highly solvent-resistant perfluoropolyether (PFPE) The elimination of multiple bonding steps also avoids the need for aligning multiple elastomeric layers and compensation for polymer shrinkage. Additionally, components can be embedded into the device in a three-dimensional fashion and pin input holes can be formed as part of the mold in situations where punching would crack brittle polymer layers. Solvent-resistant microfluidic components enable the use of organic solvents incompatible with polydimethylsiloxane (PDMS), thus opening up a vast array of potential microfluidics applications in organic chemistry and combinatorial synthesis.
The embedding of the components into the device works as follows: 1) The wax substrate is built up on the glass substrate; 2) When the mold reaches the layer where the embedded item (e.g. a filter) is to be placed, the machine is paused and the mold removed; 3) The item is melted to the wax with the use of a heater (similarly to a soldering iron) and made level with the last layer printed; 4) The mold is put back in the machine and it continues building. As the next layer is built, the deposited liquid wax is bonded to the embedded piece and becomes one with the mold; 5) The mold is processed as before, with the filter being embedded in the final polymer. This embedding process works similarly also with the parylene embodiment.
While several illustrative embodiments of the invention have been shown and described in the above description, numerous variations and alternative embodiments will occur to those skilled in the art. Such variations and alternative embodiments are contemplated, and can be made without departing from the scope of the invention as defined in the appended claims.
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CUMBERLAND ŌĆō In recognition of the townŌĆÖs strong budgetary performance and good management practices, Standard & Poor's Ratings Services has raised CumberlandŌĆÖs general obligation bond rating one notch to ŌĆ£AAŌĆØ from ŌĆ£AA-ŌĆØ.
A bond rating for a town is like a credit rating for a person. The Standard & PoorŌĆÖs Rating Services bond rating system uses an alphabetical scale from AAA - indicating an extremely strong capacity to meet financial commitments - down to D, marking an obligation that is in default.
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Madison, WIUSA 53705
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I'm interested, yes.
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Hollywood Matchmaker
Who Should Joseph Gordon-Levitt Date?
By Stacie Anthony
Joseph Gordon-Levitt captured your heart as a quirky alien on "3rd Rock From the Sun." He wowed you in "(500) Days of Summer." And now that he's starring in the new sci-fi action flick "Looper," we decided it's the perfect time to play a little game of Hollywood matchmaker. But what you may not know about this Hollywood hipster is that he believes that "making checklists of things you're looking for in a person is the numero uno thing you can do to guarantee you'll be alone forever." So we ripped up our own checklist for JGL and lined up a bevy of beauties -- like Zooey Deschanel and Elizabeth Olsen -- to see whether you think any of these leading ladies would be a perfect match for Joe. Click through to cast your vote …
Zooey Deschanel
Just like every other fan who watched "(500) Days of Summer," we're hoping that these two BFFs become an item. But what's stopping them? They've known each other for nearly 12 years, they both love music, movies and art. And they make adorable amateur music videos together -- like their rendition of "What Are You Doing New Year's Eve?". In our opinion, these besties are simply perfect for each other. Dontcha think?
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GHS gears up to host SWC music, art students
GHS to host SWC honors art,music.
Gothenburg High School will host this year's Southwest Conference Honors Fine Art Festival.
Teachers and students from six SWC schools will arrive in Gothenburg Friday. SWC schools include Gothenburg, Ainsworth, Broken Bow, Cozad, Minden, Ogallala and Valentine.
Organizers of the 2011 festival are GHS teachers. They include Ernie Blecha, choir; Tom Belanger, band; and Travis Coe, art.
Each SWC school may nominate up to 12 art students, 15 band students and 24 choir students for the honors festival.
On Friday, students will have a mural contest and a mixer activity for art students and full ensemble and sectional rehearsals for band and choir students.
Saturday's agenda includes additional rehearsals for instrumentalists and vocalists and workshops for art students.
Find the complete story in our print edition. Receive the entire issue of the Gothenburg Times on-line in PDF format each Wednesday for only $25 per year. Call 308-537-3636 to subscribe.
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The Russians Are Coming . . .to Net Lease
NEW YORK CITY-Jeff Hughes, managing director of investment sales for Stan Johnson Co. is into foreign affairs. At least as they pertain to net lease. At our recent RealShare Net Lease Conference, he detailed some deals his firm is lining up for Russian investors.
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I am on Geodon 40 mg and Lamictal 200 mg day. I cant sleep,
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I am on Geodon 40 mg and Lamictal 200 mg day. I can't sleep, my mind races, but I have no energy, can not show emotion, clench my jaws constantly and jaw moves back and forth by itself. My hand shakes sometimes. I just moved and can not get in to see a new doctor until end of Jan and do not know what to do. Am thinking about going off Gedon and decreasing Lamictal to see if it helps. I feel like I am losing my mind
3 years ago.
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MN Psychiatrist
replied 3 years ago.
Hello, I am a psychiatrist.
I feel for you, trying to deal with all of this, as if moving isn't stressful enough.
I can help you, if I knew how long you were on each of these doses of these medications. Also, are you on any other medications at all? If so, what are their doses? Lastly, what do you take these medications for?
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replied 3 years ago.
I have been on these about 2 months. I was on 160 mg of Geodon and then was decreased to 40mg. I am also on Clonazepam 0.5 3 times a day. I was dx as being
about 5 months ago and have been changed on meds so many times I do not even remember what all I have been on. I just found out I have a tumor on my pituitary gland and the doctor who is treating that said it is possible my mood swings were from that, so I wonder if I am even bipolor. I have been on antidepressants for about 15 years and was on xanax for
for about 20 yrs, but psychiatrist took me off that. My depression got out of control a few months ago and that is when he started changing my meds. I can not see my new psychiatrist until Jan 23rd and so not know what to do.
MN Psychiatrist
replied 3 years ago.
Were the jaw movements worse on the higher dose of Geodon?
Ask Your Own Mental Health Question
replied 3 years ago.
no it seems to be getting worse now.
MN Psychiatrist
replied 3 years ago.
So how long have you been on these doses of the Geodon and Lamictal? Two months at these doses, or is that how long you have been on the Geodon and Lamictal total?
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replied 3 years ago.
I think it is the total time. I was only on the 160 mg for a week or 2 and felt so sick, the doctor decreased it
MN Psychiatrist
replied 3 years ago.
It's not very common to have movement-related side effects such as jaw movement, from Geodon. I think it would be well worth calling your old doctor and asking about going off of it.
Lamictal generally doesn't cause any movement-related problems at all, but may make you feel emotionally flat.
Of the medications that you are taking, the Klonopin is by far the one most apt to make you tired.
If you go off the Geodon, you shouldn't make any other medication changes at the same time, or it will be hard to tell which medication change is causing what effect.
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Rocking blues and barbecue at Pitman's
LACONIA — Pitman's Freight Room in Laconia is hosting Rosemary and the Snakestretchers on Saturday, June 21 at 8 p.m. Rosemary Casey has been consistently rocking the New England Music scene since 2009 with her high energy, rocking R&B Blues.
Carl "Jake The Snake" Jacobsen is no stranger to the New England Blues scene as the long time front man for Jake and the Snakestretchers. A master of the guitar and vocals, Jake joined forces with Casey in 2014 and brings to the band a great energy loved by all. His "laid back" commanding style is ever so entertaining.
Admission to the show is $12, Pitman's is a BYOB venue. An optional pre-show barbecue is available for $10 and will be served beginning at 6 p.m.
Last Updated on Friday, 20 June 2014 09:37
Hits: 129
3 renowned artisans leading classes at Sandwich Home Industries
SANDWICH — The Sandwich Home Industries, (SHI) will feature three renowned artisans from New Hampshire during the first classes of the season starting Thursday, June 26 at 10 a.m. The artisans featured during the summer season are Joy Raskin, Sue Mulvey, and Sara Goodman.
Mulvey will start on June 26 at the Gallery in Sandwich, teaching forged pendants, including bezel making, soldering and cabochon setting skills. The next day, Friday the 27 Raskin will meet students at 10 a.m. instructing them in the art of wire chain jewelry constructing. Using copper, brass, bronze and silverplate, students wrap, bend, knit, hammer, and spiral their way to making chains to be worn or samples.
Finally, starting 7th with additional class dates of July 21, August 4 and 11, Sara Goodman will present a 4 class program on Learning to Weave for new weavers or those seeking a refresher. The instruction will include learning the essential elements of pattern, color and design, project planning, weaving instruction and finishing handwoven cloth: washing and fringe. Each student is assigned a loom, and is encouraged to work in between classes on these looms when the gallery is open. There will also be open studio hours by the instructor for the students to get additional help between classes.
Additional classes scheduled in the next several weeks by the Sandwich Home Industries include: Chair Caning for beginners July 10, 17, 24, 31 and August 14, Atmospheric Perspective in Pastel adult class and Kiln-fired glass sun catchers pre-teens class both held on Thursday, July 17. There are also 19 additional classes scheduled between July 18 and August 27 for adults, teens and children.
Last Updated on Friday, 20 June 2014 09:33
Hits: 91
Moultonborough Police K-9, Rony, Retires After Nearly 9 Years on the Job
MOULTONBOROUGH — It hasn't been a "ruff" life for Moultonborough Police Department K-9 Rony, but even after nearly nine years of service, he was still up-and-ready when his last day on the job came on May 30.
The 11-year-old German Shepard was born in the Czech Republic, flown to the United States and trained by Castle K-9 when he was 1 year old. Rony was then brought to the Moultonborough Police Department and first paired with Officer James O'Brien. When Officer O'Brien left the department, Rony was paired with Detective Stephen Kessler, and they have been inseparable ever since.
Kessler, who has been a member of the department since 1995, really took to his partner, and that is why Rony will be living with Kessler and his family in retirement.
"Rony has served the citizens of Moultonborough well and deserves to spend his remaining years in peace, with his handler and partner, Detective Stephen Kessler and the Kessler family," said Moultonborough Police Chief Leonard J. Wetherbee Jr.
The Moultonborough PD has decided not to train another K-9 at this time, and will retrofit the K-9 cruiser to become a part of the uniform fleet.
Rony was a multi-purpose K-9, able to track and apprehend suspects, detect narcotics, and conduct routine patrol. Kessler recalls many incidents when he and Rony were responsible for tracking down suspects after they had fled their vehicles, as well as a time when they located a suspect barricaded inside the closet of a house, leading to his arrest.
"Rony is very intelligent and he's very social, with both people and other dogs," said Kessler, who yells commands to Rony in the dog's native Czechoslovakian. "He's a very loyal companion. I'd rather work with him than most humans!"
The two officers were even part of two Special Weapons and Tactics (SWAT) teams – Belknap County Special Operations SWAT and Central New Hampshire Special Operations SWAT.
Now that Rony is retired, it doesn't mean he's slowed down. According to Kessler, who received official ownership of Rony from the town, Rony still has lots of energy, especially now that he's not using any for work.
"He's 11 years old but you wouldn't know it," said Kessler. "Rony loved coming in to work. Not having him with me 24/7 every day is kind of different. I absolutely miss him when I'm at work, but now that he's retired he's just got to hang out and be a normal dog."
Last Updated on Friday, 20 June 2014 09:25
Hits: 92
Squam Science Center hosting Coyote Day
HOLDERNESS — Squam Lakes Natural Science Center will hold a day all about coyotes on Saturday, June 28. Various activities and programs will be offered throughout the day running from 10 a.m. until 3 p.m.
The coyote, cousin to dogs, wolves, and foxes, was first seen in New Hampshire in 1944 and has spread across the state from the northernmost reaches down to the seacoast. Coyotes are currently common throughout the state and typically live for 4 to 5 years in the wild. They are intelligent and opportunistic hunters, eating a variety of animals, and sometimes working together to hunt. They are animals who adapt quickly to new situations, taking advantage of changes in their habitat.
Squam Lakes Natural Science Center has two resident coyotes. A male coyote is on exhibit in the new Coyote Exhibit on the live animal exhibit trail. A female coyote is used exclusively for programs to educate the public about the species.
There will be various activities offered throughout the day including coyote crafts for children from 10 a.m. to 2 p.m. The Science Center will also hold special Up Close to Animals presentations featuring the female coyote at 11 a.m. and 3 p.m. Coyote games will be held from 2-3 p.m. Visitors can join in an all-day scavenger hunt to win a coyote prize. Science Center volunteers will be on-hand at the Coyote Exhibit to answer questions.
In addition to the many activities, Chris Schadler from Project Coyote, will present "Becoming Wolf: The Eastern Coyote in New England." Project Coyote is a North American coalition of wildlife scientists, educators, predator-friendly ranchers, and community leaders that works to promote compassionate conservation and coexistence between people and wildlife. Project Coyote works specifically to change negative attitudes toward coyotes, wolves, and other native carnivores by educating the public about these animals. Chris will speak at 1 p.m. on Coyote Day and be available to answer questions about coyotes.
Admission is $17 for adults, $14 for seniors age 65 and over, and $12 for youth ages 3 to 15. Members and children 2 and under receive free trail admission. More information including a full schedule of events can be found at www.nhnature.org.
Last Updated on Friday, 20 June 2014 09:20
Hits: 95
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- VNA Jamboree in Tuftonboro Saturday to Benefit Central New Hampshire VNA & Hospice
- Design Your Own Garden Totem at Crafts Gallery
- Fish & Game To Talk Bass and Bass Tournaments at The Squam Lakes Association
- Hebron Gazebo announces 2014 Programs


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