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Wolves News
Longest-running wolf study is running out of wolves
Karen Graham
A predator-prey study started 57 years ago will have to end, or possibly shift direction next year because the wolves being studied are nearly extinct. The population of predators on Isle Royale is down to three lone wolves.
Op-Ed: GOP Rep. Don Young: Wolves would solve the homeless problem
Karen Graham
It seems that being a politician these days is really tough. Alaska GOP Rep. Don Young found out how the hard way when he suggested Thursday that the best way to solve homelessness was wolves. Would this be considered shooting yourself in the foot?
Two bills to strip endangered status of wolves being considered
Karen Graham
Wolves have been given a bad rap, based on a number of studies, yet despite evidence that the wolf causes less than five percent of livestock deaths in the U.S., the House of Representatives is considering two bills to take them off endangered status.
Swedish court stops hotly contested wolf hunt
By AFP
Stockholm - A Swedish court has pulled the plug on a wolf hunt due to start Friday, favouring animal rights activists in one of the country's most hotly disputed environmental issues.
Hunting triggers rise in wolves' stress levels
Tim Sandle
In territories where wolves are regularly hunted, scientists have noted a rise in hormones responsible for stress in the animals.
Review: 'Wolves' sniffs out the details
Sarah Gopaul
'Wolves' is a coming-of-age story about an uninformed werewolf accepting his true nature, meticulously constructed by a first-time director.
Man with chainsaw carves eagles, wolves, other masterpieces
Mike White
Leonardo da Vinci painted to create works of art. Michelangelo carved marble to create his works of art. Mark Tyoe of Salisbury, New York uses a chainsaw and big tree trunks to create his artwork.
Florida Fish and Wildlife officers gun down rare wolf
Justin King
Chipley - A rare British Columbian wolf named Chaco was shot and killed by Florida Fish and Wildlife officers after flooding knocked down his enclosure at Seacrest Wolf Preserve and he escaped into the countryside.
Why the last legal form of dog fighting is taxpayer-subsidized
Justin King
Madison - The practice of hounding is centuries old and represents an example of one of the earliest forms of cooperation between man and dog. In Wisconsin, it is legal to turn a pack of dogs loose on predators such as wolves and bears.
Bill seeks to remove federal protection for endangered species
Justin King
Washington - The Endangered Species Management Self-Determination Act, currently in committee in the Senate, seeks to leave protection of endangered species up to the Governors of each state and automatically remove protections for animals after five years.
Idaho game management killing elk after killing wolves
Justin King
Boise - Ranchers in Idaho are asking the state government to help eliminate some of the state's elk population. The state is halfway through the wolf season, which was said to have been introduced to stop the wolves from attacking elk.
Op-Ed: Federal judge allows wolves to be killed so hunters can take elk
Justin King
Salmon - A federal judge denied conservationist efforts to stop the trapping and killing of two wolf packs in Frank Church River of No Return Wilderness in Idaho.
Dogs evolved from wolves due to human domestication
Tim Sandle
Chicago - A new genetic study of modern canines supports the theory that humans domesticated dogs from wolves, and this happened before humans began to grow crops.
Inside the caucus for wolf activists in Wisconsin
Justin King
Madison - This weekend, activists hoping to protect Wisconsin's wolves are gathering for a caucus to formulate a strategy to better defend the wolf population in the state.
Wolves have similar social skills to dogs
Tim Sandle
According to a new study, wolves can learn from humans and have some 'social skills' that are similar to dogs. This is despite wolves and dogs being separated b over 20,000 years.
Reward for information about endangered wolf killings
Justin King
Six endangered red wolves have been shot in the last month in North Carolina, prompting the federal government to advertise a reward made up of contributions from conservationist organizations.
Yellowstone wolves possibly among those killed in Wyoming
Justin King
Recent kills by hunters in an area bordering Yellowstone National Park have biologists and wolf-watchers worried that the Lamar Canyon Pack has again been hit by wolf hunters in the area just outside the park.
Op-Ed: Graphic viral images of dying wolves spur debate
Justin King
Images of dead, dying, and injured wolves circulating on Facebook have caused an outcry of opposition and an increase in the number of those visibly opposing wolf hunting and trapping.
Op-Ed: A wolf hunter's perspective and the solution to the debate
Justin King
As the controversy surrounding Montana's wolves continues to rage, Jason Maxwell took the time to answer questions and provide a hunter's viewpoint on the issue.
TopFinds: Introducing 'lightsaber' molecules, Montana's wolf hunt
David Silverberg
Scientists discover how a new form of matter acts more like Jedi-esque lightsabers. Why Montana wants to hunt its wolves. The latest news about bullying-related deaths in Canada. Welcome to this week's TopFinds.
FWS blasted for attempt to remove protections from gray wolves
Justin King
The U.S. Fish and Wildlife Service is attempting to remove the protections of the Endangered Species Act from the gray wolf over the objections of conservationists and wolf experts.
New way to identify wolves by their howls
Tim Sandle
Researchers now believe that they can tell wolves apart by analyzing the pitch and volume of their vocalizations (studying the howl that the animal makes).
France: Wolves to be 'educated' not to kill sheep
Elizabeth Cunningham Perkins
With French farmers and environmentalists at odds over increasing numbers of wolves killing more sheep, a plan to capture and tag problem predators, 'educating' the beasts into avoidance or identifying them for quicker elimination, is up for discussion.
Russian grandmother kills attacking wolf with bare hands and axe
Brett Wilkins
Perhaps preparing to do the dirty work in preparation for Little Red Riding Hood's arrival, a big, bad wolf picked the wrong grandmother to attack and paid for its mistake with its life.
Washington wolfpack to be destroyed for hunting livestock
Marcus Hondro
A pack of grey wolves that roams the north east area of Washington State and in the south of British Columbia, is going to be destroyed. The pack has stopped hunting wildlife in favor of livestock and farmers want them killed.
Video: Woman's joyous reunion with her wolf friends
JohnThomas Didymus
A video posted to YouTube shows a joyous scene of reunion between a woman and a pack of wolves she befriended at the Polar Zoo in Salangsdalen, Norway . The wolves expressed sheer delight at seeing the woman again after she had been away two months.
Premier League: Wolves name Connor as manager until end of season
Carol Finch
Wolverhampton - Wolverhampton Wanderers have announced the promotion of assistant manager, Terry Connor, to replace Mick McCarthy. Having failed to find a permanent replacement, the club has opted to move Connor up until the end of the season.
Canadian plan to kill wolves may save caribou, upset ecologists
Tucker Cummings
In a move that Sarah Palin might support, Canadian officials have decided to start killing wolves. It is hoped that by lowering the wolf population, caribou numbers may rebound.
Ohio authorities search for escaped exotic animals
Kim I. Hartman
Zanesville - Schools have been closed and residents urged to stay indoors in one Ohio community after dozens of exotic animals were released from an animal preserve by their owner, who later allegedly committed suicide.
Wolves in France blamed for killing sheep
Lynn Herrmann
Paris - Hunted almost to extinction in France in the 1930s, the grey wolf then earned endangered species protection, making a remarkable comeback with some 200 wolves colonizing in the southern regions and now inflicting costly losses on the sheep industry.
apis-270397 apis-266794 apis-265838 apis-261803 apis-258386 apis-256959 apis-244095 apis-241981 apis-238988 apis-224026 apis-223434 apis-222477 apis-222409 apis-219658 apis-219323 apis-218330 apis-216507 apis-215509 apis-215219 apis-214683 apis-213316 apis-210584 apis-197495 apis-190298 apis-186645 apis-173646 apis-171497 apis-170605 apis-163184 apis-160955
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GOP Rep. Don Young explains how to solve homelessness using wolves. (screen grab)
The wolf enters cautiously.
Elizabeth Batt
Wolf attacks on sheep in France rose to 4,800 by August in 2014.
Juan José González Vega
A Montana wolf biologist told us he believes this wolf to be part of the Apgar Pack in Glacier National Park.
Elizabeth Batt
He returns once more and this time stays awhile.
Elizabeth Batt
Elizabeth Batt
Justin King with Gray Wolf at Seacrest Wolf Preserve
Justin King
In this final shot of the wolf, he's picked the carcass clean.
Elizabeth Batt
In France, a proposed National Wolf Plan aims to frighten hungry wolves away from sheep farms, back into remote countrysides to hunt, kill and chow down on deer, boars and rabbits.
Patrick Bell via Wikimedia Commons
Elizabeth Batt
Elizabeth Batt
Elizabeth Batt
2010 cattle predation by species Data from NASS, 2011.
An example of the finely detailed wolf make-up in David Hayter's 'Wolves'
Ketchup Entertainment
A gray wolf in Yellowstone National Park. Gray wolves are one of the world's most widely distributed mammals and the most studied large carnivore.
Jeff Burrell -Eurekalert
Jason Mamoa is the top dog in 'Wolves'
Ketchup Entertainment
Wolves. Interpretive Centre Wasagaming Riding Mountain National Park
Ken Hanly
A mass of fur from the juvenile elk littered the pasture.
Elizabeth Batt
Elizabeth Batt
Kim I. Hartman
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Reward raised to $500,000 in P.E.I. potato tampering case
EU law bans certain travel selfies that could put you in jail
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Demographers sifting through Census data have found that Jacksonville has one of the highest numbers of same-sex parents and further to that, one analyst says the data shows child rearing among same-sex couples appears to be more prevalent in the southern states.
This is according to Gary Gates, a demographer at the University of California. He claims Census results demonstrate states like Arkansas, Mississippi and Texas are more likely to have same-sex couples raising children than areas such as New York which are often perceived as more welcoming to LGBTs. This isn't the only notion Census data seems to challenge, either.
From The New York Times:
"We're starting to see that the gay community is very diverse," said Bob Witeck, chief executive of Witeck-Combs Communications, which helped market the census to gay people. "We're not all rich white guys."
Black or Latino gay couples are twice as likely as whites to be raising children, according to Mr. Gates, who used data from a Census Bureau sampling known as the American Community Survey. They are also more likely than their white counterparts to be struggling economically.
Married same-sex parents face legal hurdles. Florida does not recognize same-sex marriage, and its domestic partnership recognition, while growing, is an uneven patchwork, and still leaves many spouses uninsured.
Even when employers agree to cover domestic partners, those couples pay higher taxes, because without federal recognition of their status, health coverage is considered income and is taxable. Until recently, Florida was one of a handful of states that expressly prohibited adoption by gay couples.
Based on Census data, Mr. Gates estimates 32 percent of gay couples in Jacksonville are raising children. This is second only to San Antonio which has a rate of 34 percent. Why do southern states have a higher contingent of same-sex couples raising children then?
Mr. Gates muses that religious disapproval of same-sex relationships may come into play when he suggests that a large number of those parents had children in previous heterosexual relationships.
Gates also points out that Census data shows gay men who used to be in heterosexual relationships had children on average three years earlier than their heterosexual counterparts.
The reason for this trend is not offered, but one could guess that in an attempt to assimilate and live a heterosexual life, those individuals would marry and have children earlier.
However, the article notes that there is still a fear of not being accepted in these states, with one Jacksonville couple talking about the tough decisions they've taken due to one parent being a teacher at their child's local school:
Cynthia, the mother of a talkative 9-year-old, can be herself at her daughter's cheerleading practice, because it is far from their home. But at her daughter's school, she tells no one that she is gay, because her partner, Monique, teaches there.
Their daughter, they said, ends up with a mixed message at school.
"We tell her, 'Be honest, don't lie, but keep this in the closet,' " said Monique, who asked that the couple's last names not be used to protect her privacy at work, "It gets confusing for her."
Yet the article also highlights an optimism that the strong presence of same-sex parents in communities previously thought of as anti-gay may in fact be helping to change attitudes and demonstrate that often have more in common with their neighbors than they do differences.
Based on data collected in 2009, the Census Bureau estimates that there are 581,000 same-sex couples in the United States. The Census does not count single LGBT people, so this data collection gives only a glimpse into a demographic who has thus far remained largely invisible in official records.
Read more: adoption, census, census data, civil rights, gay parenting, gay parents, gay rights, lgbt righs, marriage equality, same-sex parents
Photo used under the Creative Commons Attribution License, with thanks to nerdcoregirl.
Oh my goodness...If we have more same-sex parents raising children in Texas and other southern states, what will become of the 'good old boy' mentality?
interesting, thanx
Tavis, those 'poor kids' are probably a hell of a lot better off than many children being raised in poor,single parent families, or even 'normal' dysfunctional families.
They are there because they are wanted, not the result of an accidental pregnancy.
If the approval process for adoption is as stringent as it is here in Australia, they have to show they will be excellent parents before they are allowed to adopt.
I hadn't thought of it that way before, but Ruby W. makes a great point.
@ TAVIS HARRISON...All I can say is...I hope you get nut cancer.
Tavis: This is a Care2 site. Key word: Care.
Tavis...I'd a million times rather have loving gay partners raise children that someone like you. I don't know what has happened to you to make you such an awful person, but you need some serious mental help or we're going to be reading about you in one of these articles one day. Or is it that you're actually bullied in real life and feel superior coming here and spouting your hate? Whatever it is, to me, you're a scary, unbalanced person and I'm glad I don't live anywhere near you. You make me want to beat the shit out of you every time you post your hate.
For clarification, I mean those poor kids for being raised by gays.
If it weren't for gay parents, no one would adopt american children or drug and alcohol abuse babies. I am glad things are changing for the GLBT community in the south and everywhere. I thought every family had gay & lesbian relatives.
I think these women are making a mistake and if it were me I move the hell out of the south and take my daughter into an environment where she would not be force to lie so one of her mothers could keep her job.
I am gay and my partner (of 21 years) and I have two daughters (my partners biological children) and 2 grandchildren. I can't imagine living like these two women. Then again you couldn't pay me to live in the South.
Disclaimer: The views expressed above are solely those of the author and may
not reflect those of
Care2, Inc., its employees or advertisers.
It's sad that the flag has been so debased in modern times when it clearly still has a historical and…
How cute ... And how funny and cute a baby elephant sounds, eh? :)
Laura needs care of the correct kind not assistance in dying. There are treatments out there (not just…

subset: commoncrawl


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'Seasons of Poetry' is Released
Jonas Ridge, NC
Poet Kenneth Ollis has assembled what he feels to be an altogether original work in his new poetry collection, "Seasons of Poetry" (published by iUniverse).
"My poetry is written in a different style from others I have seen," Ollis says. "Each poem is distinctly different. Collectively they are entirely different from other books of poetry readers are likely to encounter."
Ollis states that his poems are intended for a diverse audience. The poetry within "Seasons of Poetry" covers a range of topics and emotions, with some conveying sadness and others inspiration.
An excerpt from the poem "Old House":
"The road leading to your door, old house,
Still guarded by a rusting iron gate;
Causes you to remain visible and admired.
Passers by consider your fate.
The landscape would seem void without you,
Although your magnolias and willows are no more.
Your once manicured lawns and lovely gardens
Have no caretakers as before."
"Seasons of Poetry"
By Kenneth Ollis
Hardcover | 6 x 9 in | 108 pages | ISBN 9780595657186
Softcover | 6 x 9 in | 108 pages | ISBN 9780595279067
E-Book | 108 pages | ISBN 9781469748894
Available at Amazon and Barnes & Noble
About the Author
Born in 1935, Kenneth Ollis is a native of North Carolina. He and his wife have two sons and three grandchildren. He is the author of "How Blue Are the Ridges."
iUniverse, an Author Solutions, LLC, self-publishing imprint, is the leading book marketing, editorial services, and supported self-publishing provider. iUniverse has a strategic alliance with Indigo Books & Music, Inc. in Canada, and titles accepted into the iUniverse Rising Star program are featured in a special collection on BarnesandNoble.com. iUniverse recognizes excellence in book publishing through the Star, Reader's Choice, Rising Star and Editor's Choice designations-self-publishing's only such awards program. Headquartered in Bloomington, Indiana, iUniverse also operates offices in Indianapolis. For more information or to publish a book, please visit iuniverse.com or call 1-800-AUTHORS. For the latest, follow @iuniversebooks on Twitter.
About Author
- Casting Roundup: Sarah Chalke, Kim Raver Headline New Pilots
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1-year-old struck, killed by 18-wheeler
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2016 Acura MDX — a new nine-speed automatic transmission is the big change.
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To mark the 20th anniversary of the Light's last edition, we've combed through the archives to bring you 100 Light covers chronicling historic events.
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GOOD KNIGHT everybody!
Easy One. Even for Johny Bravo.
Yoy don't need the queen to win. SOMETIMES !
what is if 1st page??????????????????
as easy as yesterdays
I need to be able to see this in a match! First page too...
first page?
Love it! :)
What if you first move the bishop and only then a horse?
I made it!
First page, woohoo!
1st page!!!! Are you kidding me??? Oh my gawd…oh my gawd…wait…oh…oh..ooooooooohhhhhhhh….uh oh…oh damn, I just creamed myself…because getting on the 1st page is such an enormous accomplishment…I mean, I do have to be awake when the puzzle changes…quite a feat…takes immense talent and skill…and now, I'm one of the very few select thousands of people to do ever do it! Wait a minute, wait a minute….ohhhhhh I creamed again just thinking about it…this is so much better than actually getting laid…I mean, I imagine…I've never really been laid…with anyone besides myself that is…I could I suppose, but I just don't have time…I have to focus my time and energy on getting on the 1st page…I'm so awesome!
Well, you got there. Time to set new life goals old man.
there are alot of people up?
Should chess be considered a sport?
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New England Cable News' The Morning Show published a transcript of an interview with newly appointed Massachusetts Public Safety Secretary on School Security Andrea Cabral. Here's what she had to say about her new position.
Read more
An area church rallied outside the Onslow County Courthouse and the Onslow County School Board building this week, on a mission to put prayer back in schools.
Flour Bluff High School is one of 60 schools nationwide set to offer a pilot course in computer science and software engineering as part of programming from national curriculum provider Project Lead the Way.
Westport's Board of Finance tonight unanimously approved a $50,000 appropriation request from the Board of Education for a school security study in the wake of last month's Newtown school shooting.
On a normal day at the Academy of Business, Law and Education charter school in Stockton, students will enter their classrooms and perform a 10-minute warm up assignment as part of a 90-minute course block.
Local and state education officials have begun discussions about merging the Monticello Independent school district and its 850 students into the Wayne County school system.
It was back to school for city students Wednesday, including some Queens kids who are now back in their own schools for the first time since Hurricane Sandy.
Students and teachers gathered outside the Sawyer School in Hartford on Wednesday morning, angry over the school's sudden closure during holiday break.
A New Jersey town added a fourth R to its curriculum: Revolvers.
The Lake Travis School District's board of trustees has selected Bartlett Cocke General Contractors for the $42 million construction of the new Lake Travis Middle School.
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Tag: unplugging
In our wired world it's easy to extol the virtues of the web, mobile devices and social media. These time-bending technologies have generated previously unimaginable ways of searching, creating and sharing information instantaneously. Yet as our hours and days are filled with more and more time-saving devices, many of us seem to have less and […] more »
Since October I've ventured to 13 cities in 9 countries. My passport now has stamps on it from Kenya, Mexico, Jordan and India, to rattle off a few. And it's gotten to the point where my apartment is not a living space but a refuge for clothes that either just emerged from my suitcase or […] more »
Before my son started kindergarten in a public school in Boulder, Colo., in August, his teacher asked me to bring him in for an assessment. I expected this to be similar to what my daughter experienced when she started kindergarten three years ago — he'd meet his teacher, see his classroom, and then his teacher […] more »
I reached into my pocket after class to grab my phone. It was a habit I had established early on in the semester after learning that my history lecture was a tech-free zone. But this time when I reached into my pocket, my phone wasn't there. At the tail end of the semester that marks […] more »
There is an old lesson that often reverberates through my head; one that says that when you are old and your kids are grown and out of the house, you aren't going to remember how clean the bathroom was or that all the dishes were always washed and put away. You are going to remember […] more »
The holidays are upon us and you've probably done your fair share of running around. But can you truly get away from the office? Laptops, tablets and cellphones have made working on-the-go that much easier. Sometimes bringing all of your devices with you can feel like a necessity even after you clock out. According to […] more »
Taking a break from liking, friending, following, pinning, surfing, posting and all those other online pursuits is daunting enough for regular users. Imagine being a technology pioneer who, by definition, has to stay wedded to the web. This past year, a number of big names in the digital world took on this exact challenge. Paul […] more »
In a single day, the average American spends eleven hours with digital media. With so many ways to stay connected, it can be difficult to power down the screen and engage with the world beyond it. But sunset on Friday, March 7, offers an excuse to get off the grid. The National Day of Unplugging, […] more »
I can remember the moment I realized that smartphones were going to change our relationships. I was at a 40th birthday dinner party for a friend, and as I looked around the table, 27 of the 28 guests had their faces buried in their iPhones for most of the night. Yes, they would look up […] more »
Call it spring cleaning. A digital detox. Each year, even we, those of us who live, eat, sleep and breathe online, need a little break (perhaps especially us). Enter, the National Day of Unplugging — a reason for and a celebration of a "technology Sabbath." This year, the National Day of Unplugging, advocated by the group […] more »


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Alan's Airport Transfers & ToursAlans Airport Transfers & Tours offers a luxury door-to-door service to the corporate and leisure traveller. Alan Perring's service is personalised and convenient and offers passengers peace of mind both day and night with no late night or early morning surcharges.
Your #lovecapetown weekend: What's on from Wednesday 01 July to Sunday 05 July, 2015
Rob PetersWith a wealth of natural beauty, unrivalled mountain trails and sublime backdrops, it's not hard to understand why Mother City locals spend the majority of their time outdoors and on the go. The good news is that it's even better in winter with the cooler temperatures making it an awesome option for sport and fitness fanatics!
Make a difference on Mandela Day in Cape Town
Rob PetersMandela Day 2015 on July 18 marks what would-have been our beloved Tata Madiba's 97th birthday and it's the ideal opportunity to follow this great man's example by channelling your inner charitable spirit. Here are 10 ways you can get involved in the Mother City.
@lovecapetown twitter talksWe love hearing about Cape Town from those who are here, who have been here and want to be here. It is for this reason that we occasionally host a twitter chat and we want YOU to get involved! Here's how.
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UCT's Role in the Struggle
UCT stands out as an important case study for the development of effective and radical student protest during the Apartheid Era.
Iconic Cape Town institutions on Valentine's Day
Our amazing city has once again shown that it has brains and beauty, by celebrating Valentine's Day on one of the most iconic places in South Africa – Robben Island.


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Top Stories
- Comptroller: state payroll system antiquated
- Remember, fireworks are dangerous
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- Dispute over state payroll rolls on
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Youth Track: Rockton seventh-grader wins state track title
May 23, 2012
Staff Report
ROCKTON, Ill. — DuWayne Patriq Jones of Stephen Mack Middle School in Rockton, Ill., had a great weekend at the Illinois Elementary School Association (IES) state finals.
Jones placed first in the 400-meter dash for the IESA seventh-grade boys' division with at time of 53.51. He made it to the semi-finals in the 100-meter dash, and placed 12th overall, just missing the finals with a time of 12.58. He was also the anchor leg in the boys' 4-by-400-meter relay that placed third overall in state with a time of 4:03.
Overall, the Stephen Mack Middle School team did well and is bringing home seven medals and one state championship title.
From the May 23-29, 2012, issue


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Americans, Politics and Science Issues
The general public's political views are strongly linked to their attitudes on climate and energy issues. But politics is a less important factor on biomedical, food safety, space issues.
Americans' Internet Access:
The share of all U.S. adults who use the internet increased from 52% in 2000 to 84% today.
Americans' Attitudes About Privacy, Security and Surveillance
Many Americans want control over their personal information and freedom from observation during the activities of their daily lives, but they are not confident that the government agencies or businesses that collect data about them can keep that information private and secure.
Americans' Views on Open Government Data
Many hope that more transparency and data sharing will help journalists, make officials more accountable and improve decisions. But very few think agencies are doing a great job of providing useful data.
Teens, Social Media & Technology Overview 2015
Smartphones are fueling a shift in the communication landscape for teens. Nearly three-quarters of teens now use smartphones and 92% of teens report going online daily — including 24% who say they go online "almost constantly."
U.S. Smartphone Use in 2015
Nearly two-thirds of Americans now own a smartphone. 19% of Americans rely to some extent on a smartphone for internet access, but the connections to digital resources that they offer are tenuous for many of these users.
Public Libraries and Hispanics
Hispanic immigrants are less likely than U.S.-born Hispanics, whites and blacks to use public libraries. But Hispanic immigrants who have made their way to public libraries stand out as the most appreciative of what libraries have to offer.
Americans' Privacy Strategies Post-Snowden
Nearly two years after Snowden's revelations, 87% of Americans say they have heard about U.S. surveillance programs. Among them, 25% say they have changed their own technological behaviors in some way.
How Scientists Engage the Public
Most scientists (87%) believe it is important to participate in public policy debates. Almost half use social media to discuss or follow science, and nearly a quarter blog about science and research.
How Different Groups Think about Scientific Issues
Different demographic groups think differently about scientific issues. For example, those more likely to think genetically modified food is unsafe include women, African-Americans and Hispanics, and those without college degrees. Those more likely to say parents should be able to decide whether to vaccinate their children include younger adults, Republicans and independents.


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VIEWS: 13 PAGES: 1 POSTED ON: 7/24/2011 Public Domain
Energy Efficient Mortgage Worksheet (Sample) Borrower Name: FHA Case Number: Property Address: Lender Loan Number: A. Property 1. Contract Sales Price 2. Original Sales price if 3. Appraised Value 4. Weatherization &/or Information or ☐ Existing Balance owned < 12 months (Note 1) solar not yet included in $ $ $ A2, A3 or A4 (Note 2) $ B. Home Energy 1. Cost of improvements that 2. Installed Cost of Energy 3. Expected energy savings over Rating System exceed 2000 IECC Improvements useful life of improvements (HERS) Report (New Construction Only) (Exisiting Construction) (calculated at Present Value) per $ $ audit report $ C. Cost-effective 1. Installed cost of EE improvements (B1 or B2) $_____________ Energy Package + audit cost $__________ + inspection(s) cost $___________ (all costs supported by receipts) $ 2. Maximum amount of EE improvement cost per the EEM calculator in FHA Connection (Note 3) $ 3. Lesser of C1 or C2 (this is the cost-effective energy package and the amount that may be added to the base loan amount) (Note 4) $ D. Eligible 1. Base loan amount from Loan Transmittal Mortgage (without EE Costs and without UFMIP) (Note 4) $ Calculation 2. Solar system cost allowance $ 3. Cost-effective Energy Package (C3) $ 4. Total base EEM loan amount (D1 + D2 + D3) $ 5. UFMIP (factor applied to D4) $ 6. Total combined loan amount (D4 + D5) (Note: Only whole dollar amounts may be insured by FHA) $ E. Qualifying 1. Mortgage amount used for qualifying ratios Ratio (D1 $____________ + UFMIP based on D1 $__________) $ Calculations 2. Total monthly housing payment for E1 + E2 (PITI _______, HOA _______ monthly MI ________, other/etc._______) $ 3. Total monthly obligations $ 4. Housing-to-Income (Front) Ratio % 5. Total Debt-to-Income (Back) Ratio % Either New or Existing Construction: Does the HERS report indicate that the subject property was built to or retrofitted to 2000 IECC Yes ☐ No ☐ standards? (Note 6) Underwriter Comments Notes: 1. Tips: a) For streamlined refinances without an appraisal, CHUMS and FHA Connection uses the appraised value in its records from the prior transaction. b) For EEMs with 203(k), use After-Improved Value for value. c) Apply rules related to second appraisals for properties flipped by seller. 2. a) Weatherization items apply only to existing properties. b) Weatherization cannot be used in conjunction with streamline refi, as this would be considered cash-out. c) The amount of energy-related installed cost that exceeds maximum EEM calculation in FHA Connection, may be used under weatherization policies described in HUD Handbook 4155 A.5 (d-g). d) Any weatherization costs used must be added to both sales price (for purchase) and the appraised value when calculating the maximum mortgage amount. e) A modified price/appraised value that includes weatherization items is still subject to statutory limits for a given area. f) Solar guidelines may be found at 4155.1 2.A.5. 3. Least of: (i) 5% of appraised value, or (ii) 5% of Median Area Price x 115%, or (iii) 5% of National Conforming Loan limit x 150% 4. Base loan amount must consider applicable guidelines (examples: acquisition cost if owned < than 1 year, minimum required cash investment for purchases, new construction properties < than 1 yr old, streamline refinance, etc.) 5. The amount of installed cost that exceeds C2 may be financed with the Weatherization Program (existing properties only), or paid from borrower's own funds. 6. If evidence is provided that the property was built to, or retrofitted to 2000 International Energy Conservation Code (IECC) standards, the loan is eligible for "stretch" qualifying ratios (33 percent and 45 percent) before the need for compensating factors. 6/25/2010
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|Blog Posts
Patients with a biological valve may need of 10-15 years. Mechanical valves may also fail, so patients should inform their doctor if they have symptoms of valve insufficiency. Patients with mechanical valves must take blood-thinning medication at the end of their lives. Because these medicines increase the risk of bleeding in the body, you should always be used in medical notification bracelet and the doctor or dentist that you are taking anticoagulant medicine. Even if you take anticoagulant medication, you should always inform your doctor and dentist to have had valve surgery. If you have any surgery or dentistry, you should take antibiotics before the procedure. The bacteria can enter the bloodstream during these procedures. If bacteria enter the repair or artificial heart valves, can lead to a serious condition called bacterial endocarditis. Antibiotics can prevent bacterial endocarditis.
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Originally written on Puck Drunk Love | Last updated 5/24/13
The Chicago Blackhawks have had an incredible 2013 campaign. Unfortunately, all of their success is unraveling in the second round of the playoffs against the Detroit Red Wings. The best proof demonstrating Chicago's absurd regular season success as well as their turmoil against Detroit is the fact that their 2-0 loss in Game 4 marked the first time the team was shut out in 2013, as reported by Elliotte Friedman. Stop for a moment and think about how absolutely ridiculous this statistic is. In 48 regular season games the Blackhawks never came up empty. They always found a way to light the lamp. In fact, it often became expected that the Blackhawks would find a goal and find a way to win. This expectation has faded in the postseason, specifically in the second round against the Detroit Red Wing. Chicago found their usual success in Game 1, cruising to a 4-1 victory. Then the tide turned and Chicago now finds itself one game away from starting their summer. Detroit has rattled off 4-1, 3-1 and 2-0 victories against Chicago, giving the Blackhawks their first three-game losing streak in 2013. First time being shut out. First time posting a three-game losing streak. Chicago is picking a bad time to start playing their worst hockey of 2013. [follow]
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This is above all a book about the individual. Individuals create societies and make them vital; and individuals bring them to moldering ruin. So this is a book about you.
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John Gardner lives in Stanford, California. He is the author of Morale and the editor, with Francesca Gardner Reese, of Quotations of Wit and Wisdom, both available in Norton paperback.
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Fire On Oil Rig In Gulf Of Mexico Leaves 11 Injured
Originally published on Fri November 16, 2012 1:33 pm
Update at 1:26 p.m. ET. No Confirmed Deaths:
The U.S. Coast Guard tells WWLTV that 11 people have been sent to hospital but no deaths have been confirmed in a oil rig fire off the coast of Louisiana.
WWLTV, KHOU and Reuters were reporting two deaths earlier.
Our Original Post Continues:
A fire on an oil rig off the coast of Louisiana has killed two people, Louisiana's WWLTV is reporting.
Houston's KHOU reports that a spokesman for Black Elk Energy, who owns the platform, said four workers were airlifted to a hospital and two others are missing.
"The U.S. Coast Guard in New Orleans confirmed the fire early Friday morning.
"Jefferson Parish Councilman Chris Roberts says the platform in question is a shallow water platform located in West Delta Block 32 in the Gulf of Mexico.
"The Coast Guard activated a 'command center' to investigate the incident."
Update at 12:27 p.m. ET. Fire Extinguished:
The AP just moved this bulletin:
"Coast Guard: Fire extinguished at Gulf of Mexico oil rig where 4 hurt, 2 may be missing."


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Get ready for the 2015 Pioneer Days Grand Parade in Cle Elum, Washington on Saturday, July 4th! The Parade Starts at 10:00am
90 Entries last year!
Route: North on Oakes Ave., East on First St. to Peoh
Please contact: Jessica Tate, Kittitas County Chamber of Commerce at (509) 925-2002 or email@example.com for a parade entry form. or Kerri Farnum of Vision Cle Elum at (509) 304-8222 email@example.com
Dont miss the Kids Patriotic Bike Parade- (Line up on Railroad St. by Wells Fargo bank at 9:00am)
Following the parade: Pioneer Queen's Tea
(Carpenter Family Historical Home & Art Gallery)
Heritage Festival
Ethnic food and dancing hosted by Ronald Community Club
5:00pm-9:00pm Hawthorn Hall- Ronald
$6.00 Cover Charge
The Professional Firework show will be at 10pm, downtown.
Several members of the Hills Orchard Improvement Group, which promotes the work of more than 130 fruHundreds Attend Wellness Hour To Bid Farewell To Ken Briggs
When Ken Briggs played school as a little kid growing up in Denver he was always the teacher. Now thGlobal Experience Distinguishes CWU's New Aviation Chair
From India, to Singapore, to Saudi Arabia, to the United States, to the United Arab Emirates, Sundar


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Shows how to create a bumper graphic and an identity spot that are animated to music, and transition the tools and techniques to your own projects.
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John Myers is tweeting that Whitman campaign has released a bunch of documents that the campaign says prove Whitman didn't know that her housekeeper was undocumented.
Whitman doc dump…
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Minor League Report: Jesse Biddle did not have a great start in a 10-0 Reading loss.
Representing the Phillies at a June public event, it was clear the Wampum Walloper's sinewy body was softening.
Pete Mackanin will continue as interim manager until season's end, but then the question will be who is next.
The Phillies on Thursday placed righthander Aaron Harang on the 15-day disabled list.
Teams around baseball will use at least part of this month to decide if they are buyers or sellers.
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Cholmeley Cr - by onefinestay
from £456 /night help Price for guests, Nights approx:
from £456 /night help Price for guests, Nights approx:
Estimated nightly price based on a weekly stay. Contact the advertiser to confirm the total cost.
House / 5 bedrooms / sleeps 10
Availability Your dates are available
House / 5 bedrooms / sleeps 10
|Key Info
If you've children, then you'll know a true family home the very second you lay eyes upon it. Sharp edges have been smoothed away, colours pulse with warmth, the walls bustle with photos and cheerful artwork, and, perhaps most importantly of all, there's oodle upon oodle of space.
And so it is with Cholmeley Cresent, an enormous home in leafy Highgate, with beds for up to ten, the most lavish of toy boxes and even a trampoline at the end of the garden. You're only in zone 3, but so quiet is the area, you'll feel very far away from the urban grind. Yet, fear not, for whether it's a trip to the (free!) museums, or a whirl on the London Eye, central London is only twenty minutes away
|Sleeps up to 10, 5 bedrooms
|Will consider
|Corporate bookings, Long term lets (over 1 month)
|Car not necessary
|Family friendly
|Great for children of all ages
|Some pets are welcome - please contact the owner, No smoking at this property
Features and Facilities
|Central heating, TV, Telephone, Wi-Fi available
|Kettle, Toaster, Iron, Hair dryer
|Cooker, Microwave, Fridge, Freezer, Washing machine
|5 bedrooms, 2 bathrooms of which 2 Family bathrooms
|Linen provided, Towels provided
|Private garden
|Not suitable for wheelchair users
|Further details
Property Manager: onefinestay redefines the experience of visiting London and New York: live like a local by staying in a distinctive home while the owner is out of town, with a service which offers all the convenience and comfort of a hotel. Our guests enjoy luxury amenities like 5* hotel linens and toiletries from The White Company in London, and Kiehl's in New York as standard. Every guest is also lent an iPhone for the duration of their stay which is stocked with local tips from the home's owner, and from which they can make free local calls.Services: Of course, we always go the extra mile. Just like a hotel, we're always available at the end of a phone while you're in town. Whether you need an extra toothbrush, a new hair dryer, a top tip for a West End show or a nanny for the night, just give us a call.
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Central London/Zone 2
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Originally written on Steelers Depot | Last updated 10/6/11
CHICAGO - SEPTEMBER 20: Chris Kemoeatu #68 of the Pittsburgh Steelers adjusts his helmut during a game against the Chicago Bears on September 20, 2009 at Soldier Field in Chicago, Illinois. The Bears defeated the Steelers 17-14. (Photo by Jonathan Daniel/Getty Images)
Steelers offensive coordinator Bruce Arians spoke to the media per his usual Thursday session and Ken Laird has the transcript up for you to read. Arians told the media that left guard Chris Kemoeatu is dealing with a number of injuries right now that include his balky knee and a bad hand. Arians stated in the interview that Kemoeatu likely would not have played Sunday against the Texans if the Steelers had other healthy options heading into that game. He went on to speculate that now that they have enough healthy bodies that Kemoeatu could be out a few weeks to give him a chance to get healthy. The Read more [...]
Steelers LG Chris Kemoeatu Could Miss A Few Weeks is a post from: Steelers Depot
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text: on Apr 13, 2013 @ 8:46 AMApr 13, 2013 @ 8:46 AM Published Russia hits 18 US officials with tit-for-tat entry banRussia on Saturday published its own blacklist of US officials banned from entering the country in retaliation for Washington blackballing 18 Russians over alleged human rights abuses.Already-strained relations between the two countries chilled further as Russia hit back at what it called an "unfriendly" move by Washington that the foreign ministry warned would hurt mutual trust.Among the 18 officials sanctioned by Russia were former Bush administration aide John Yoo, former CIA lawyer David Spears Addington, two former Guantanamo prison chiefs and officials who prosecuted convicted arms smuggler Viktor Bout."The war of lists is not our choice, but we cannot ignore outright blackmail," the Russian foreign ministry said in a statement.On Friday the US Treasury released a list barring 16 Russians allegedly linked to the death of jailed human rights lawyer Sergei Magnitsky, as well as two Chechens tied to other alleged rights abuses, from travelling to the US or holding assets there under the 2012 Sergei Magnitsky Act.The measure infuriated Moscow which had vowed to retaliate with a similar measure. The foreign ministry on Saturday called the Magnitsky Act an "absurd" law that "intervenes in our domestic affairs" and "delivers a strong blow to bilateral relations."While the US list mostly targeted mid- and low-level interior ministry officials which were involved in the case against the lawyer, Russia chose several names already known internationally due to torture allegations against them."Unlike the American list, which is formed arbitrarily, our list primarily includes those who are implicated in legalisation of torture and perpetual detentions in Guantanamo prison, to the arrests and kidnapping of Russian citizens," the ministry said."Politicians in Washington should finally realise that it is futile to build a relationship with a country like Russia in the spirit of mentoring and undisguised dictating," it said.The list names four people allegedly implicated in Guantanamo abuses, including Addington, who served as chief of staff to former vice president Dick Cheney, Bush aide Yoo, and former Guantanamo heads Geoffrey Miller and Jeffrey Harbeson.Another 14 people are named as having violated the rights of Russian citizens abroad. They include US District Judge Jed Rakoff and several prosecutors from his district in the state of New York, as well as several Drug Enforcement Administration officers and FBI agent Gregory Coleman.Most of the Americans in the ministry's "stop-list" had been involved in cases against Russian arm smuggler Viktor Bout and drug-trafficker Konstantin Yaroshenko in the US, both of which were hotly contested by Moscow. Senior lawmaker Alexei Pushkov, who chairs the Russian Duma lower house foreign affairs committee, announced a "visa war" on his Twitter blog. "The reset is dead," he wrote minutes after the ministry announcement, referring to the US-Russia policy of resetting relations launched in 2009.Deputy Foreign Minister Sergei Ryabkov told the Itar-TASS news agency that the Russian blacklist also includes a secret section which has more names, similar to a list of more highly placed Russian officials compiled by Washington."The list ... also has a closed section," Ryabkov said. "The Americans know about its existence."Sergei Magnitsky died in pre-trial detention in 2009 at the age of 37 after being arrested and charged by the very same officials he had accused of organising a $230-million fraud scheme.The case has come to symbolise the Kremlin's failure to crack down on corruption and has prompted a crisis in US-Russia ties.Moscow reacted with anger when the Magnitsky Act was passed last year under which scores of Russians -- whose names have not been released publically -- were hit with sanctions and blacklisted from receiving a visa to the United StatesIn retaliation Russia passed a law which punishes alleged US rights abusers, but also includes a clause banning adoption of Russian children by Americans.President Vladimir Putin had justified the legislation by the need to respond to a "purely political, unfriendly act" which has "sacrificed Russian-American relations."The law, and the adoption ban, went into effect on January 1 despite massive protests in Russia and international criticism.ma/fb


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If I take roxycodone in the morning how long do i need to wait before starting subutex to get of opiates
How many hours do I have to wait to take subutex after taking oxycotton?
- Posted:
- 22 Dec 2009 by smokt1
- Topics:
- subutex, opiate dependence
Answers (2)
22 Dec 2009
Roxicodone is a short acting opiate with a short half life. I would make sure that you are in withdrawals before you start or you will put yourself into precipitated withdrawals and believe me you don't want that. So I would wait until at least the next day. Make sure you are feeling really shitty and then take 4 or 8mgs. You'll know if it's working within a hour... Good luck... Dave
8 Oct 2011
From what I understand, having come off opiates, which I had taken for chronic pain in ever increasing doses, for several years, I weaned myself down with the help of a hospital and then, when the weaning process was over, they started me on subutex (no waiting period as subutex is only buprenorphine, which is in the opiod pain killer family, with a few differences. It's when the other substance, nalaxone, an opiod agonist, is added to buprenorphine to create suboxone, that a person taking it risks going into withdrawal, if they take opiates at the same time and, more specifically, it was designed to stop the person taking suboxone intra-veinously (addicts,) much like the drug many alcoholics who want to quit drinking take called antabuse, which, if they drink on it, will make them very sick. So - no worry about how long ago you took roxycodone.
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1 answer • 27 Feb 2011
Need to know how much suboxone is needed and how long do I need to take it untill Im sub free?
I have been using roxys for over a year now and I'm finally through. I obtained a few subutex as well as suboxone. Im thinking of taking the ...
4 answers • 25 Sep 2011
Do you have to wait between when switching from Subutex to Suboxone?
I am currently taking Subutex, I want to switch to Suboxone. Does anyone know if you have to wait a couple days after your last Subutex dose before ...
2 answers • 11 Oct 2011


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How to Get Mortgage Modifications After Losing a Job
Unemployment is common during tough economic times. When things don't improve quickly, some homeowners have trouble paying their mortgage loans. There's always the option of refinancing a home loan and lowering the payment. Unfortunately, losing a job disqualifies some homeowners, and mortgage lenders require steady employment. If unable to refinance, consider a modification, wherein you're able to obtain better mortgage terms without a "refi."
Things You'll Need
- Income statements
- Hardship letter
Speak with your lender--the institution where you send monthly mortgage payments. Act quickly after losing a job to contact your lender for help. Express your desire to alter your mortgage term through a modification and discuss your eligibility.
Have your income statements on hand. Mortgage modifications are purposed to help homeowners in distress. After losing a job, show proof of your current income: perhaps an unemployment check stub or bank statements detailing monthly deposits.
Demonstrate an inability to pay your mortgage. Some lenders offer modifications only to homeowners who are 30 to 90 days delinquent on their mortgage. Research your lender's requirements.
Write a hardship letter. Your hardship letter determines whether your lender will approve your modification after losing a job. State your reasons for seeking a mortgage modification, such as loss of income or employment. Provide specific details about your financial standing, and include information about your credit card balances, savings, tax returns and other loans.
Tips & Warnings
- Write an honest hardship letter. Lying about your income or trying to hide assets can disqualify you.
- Photo Credit mortgage image by hans slegers from Fotolia.com
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7 years of experience
7 years of experience
South of Market
1500 Owens St
# 170
San Francisco, CA 94143 Phone number & directions
1500 Owens St
# 170
San Francisco, CA 94143 Phone number & directions
License & Identifications
A medical license is required for a doctor to practice medicine in a particular state. Requirements vary by state but most require, at a minimum, post-graduate training in the doctor's specific specialty. An NPI number is a national identifier unique to an individual health care provider and is managed by the Center for Medicare & Medicaid Services (CMS).
Learn more about Dr. Bryan Werner:
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Specialties & Research
Dr. Bryan Werner, MD is board certified in Physical Medicine & Rehabilitation.
- He has special expertise in 1 area:
- Postural Therapy
Education & Awards
Dr. Werner graduated from University of North Carolina, Chapel Hill.
Dr. Werner has 4 hospital affiliations:
- UCSF Medical Center / Moffitt-Long Hospitals
- UCSFMount Zion
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Dr. Bryan Werner practices Physical Medicine & Rehabilitation near San Francisco, CA.
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Vt woman charged with forcing marijuana on sonMarch 28,2014
ST. JOHNSBURY A woman and her boyfriend are facing charges they forced the womans 12-year-old son to smoke marijuana at their Groton home.
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Eukaryotic translation initiation factor 4-gamma 1 (EIF4G1) gene mutations have recently been reported in autosomal dominant, late-onset Parkinson's disease (LOPD). We carried out genetic analysis to determine the prevalence of EIF4G1 variants in an ethnic Chinese population and to better understand the association between EIF4G1 and PD.
We conducted a comprehensive genetic analysis of EIF4G1 in a cohort of 29 probands of autosomal dominant, LOPD families. Polymerase chain reaction (PCR) analysis and sequencing was carried out of the entire EIF4G1 exonic regions and exon-intron boundaries. Specific mutation and exonic variants were chosen for further sequencing in a case–control study including 503 sporadic PD and 508 healthy controls. Statistical significance was analyzed by the Chi-square test.
Our analysis revealed three exonic variants (rs2230571, rs13319149 and rs2178403) and eight intronic variants across the entire EIF4G1 gene. No reported mutations were detected in EIF4G1 exonic regions. The synonymous coding variant rs2230571 in exon 27 and the eight intronic variants were not used for further sequencing, but the specific mutation c.3614G > A (p.R1205H) and the two nonsynonymous variants (rs13319149 and rs2178403) were chosen for further analysis in a case–control study. None of the 503 sporadic PD or 508 healthy controls carried p.R1205H, and there was no statistical significance in rs2178403 genotype or allele frequencies in EIF4G1 between the PD cases and the healthy controls (p = 0.184 and p = 0.774, respectively; Chi-square test). The rs13319149 genotype in all PD cases and healthy controls was GG.
Our data indicate that in an ethnic Chinese population, the pathogenic mutation p.R1205H in EIF4G1 is not common and that EIF4G1 exonic variants rs2178403 and rs13319149 are not associated with PD. EIF4G1 does not appear to be a frequent cause of PD in this ethnic Chinese population.
Keywords:EIF4G1; Parkinson's disease; Ethnic Chinese
Parkinson's disease (PD) is the second most common neurodegenerative movement disorder and is clinically characterized by the cardinal features of bradykinesia, resting tremor, rigidity, postural instability and responsiveness to dopaminergic therapy. Most PD patients are sporadic but approximately 5–10% are familial [1]. Although the etiology of PD still remains elusive, certain genes are known to influence PD susceptibility, for example, autosomal dominant PD is associated with SNCA[2], LRRK2[3], UCHL1[4] and VPS35[5,6], recessive parkinsonism with PRKN[7], PINK1[8] and DJ-1[9], and more complex forms of recessive parkinsonism with ATP13A2[10], PLA2G6[11] and FBXO7[12]. Several techniques have been used to identify candidate genes in familial PD, including classic linkage analysis, positional cloning studies and recent exome sequencing [2,5,6,13].
Recently, Chartier-Harlin et al. [14,15] performed genome-wide linkage analysis of a multi-incident French family presenting with autosomal dominant, late-onset PD (LOPD). They found that the mutation c.3614G > A (p.R1205H) in EIF4G1, encoding eukaryotic translation initiation factor 4G1 (eIF4G1), a component of the translation initiation complex eukaryotic translation initiation factor 4F (eIF4F), caused the familial LOPD. The translation initiation complex is a large family [16,17], including eukaryotic translation initiation factor 4E (eIF4E) and eukaryotic translation initiation factor 3e (eIF3e) that interact with each other and conduct normal translation initiation activity [14,18,19]. In the Chartier-Harlin study [14], it was predicted that mutations p.A502V and p.R1205H disrupt eIF4G1 binding to eIF4E or eIF3e, resulting in PD. Subsequent sequencing and genotyping analysis also identified different EIF4G1 mutations in affected subjects with familial parkinsonism and idiopathic Lewy body disease in different populations.
To obtain a comprehensive view of the prevalence of EIF4G1 variants in an ethnic Chinese population, we conducted a comprehensive genetic screening of EIF4G1 in a Han Chinese group of subjects.
The study comprised 29 probands of PD families compatible with autosomal dominant inheritance (two or more PD cases in at least two consecutive generations) and 503 sporadic PD patients of Chinese Han ethnicity from Hunan, Hubei, Jiangxi, Sichuan and Chongqing provinces of China. All patients were consecutively recruited from the Department of Neurology of Xiangya Hospital (Central South University, State Key Laboratory of Medical Genetics of China) and the Neurodegenerative Disorders Research Center. A standard clinical neurological examination was performed for all patients by two neurologists, and a diagnosis of idiopathic PD was made according to the United Kingdom PD Brain Bank Criteria [20].
There were 13 male and 16 female PD patients among the 29 autosomal dominant, LOPD probands, with a mean age at onset of 50.33 ± 9.56 years (range, 41–63 years). Of the 503 sporadic PD patients, 269 were male and 234 were female with a mean age at onset of 55.25 ± 12.21 years (range, 22–81 years). The case–control study also included 508 healthy controls (268 males and 240 females; average age at examination, 56.95 ± 15.20 years, range, 25–88 years) matched for age, gender, ethnicity and area of residence. The control subjects were healthy people from the Xiangya Hospital Medical Center, and healthy volunteers with no history of neurodegenerative diseases. A standard clinical neurological examination was performed on all control subjects, and a diagnosis of possible idiopathic PD was excluded according to the United Kingdom PD Brain Bank Criteria. There was no statistically significant difference in age or gender between patients and controls (p > 0.05, using Chi-square test). The study protocol was approved by the Ethics Committee of Central South University and written informed consent was obtained from all patients and controls.
Genetic analysis
Genomic DNA was extracted from peripheral blood using standard protocols. Polymerase chain reaction (PCR) analysis was carried out using 20 primer pairs (Additional file 1: Table S1, available online), and each PCR product was purified and directly sequenced in both forward and reverse directions on an ABI 3100 automated sequencer (Applied Biosystems, Foster City, CA). We initially sequenced the entire EIF4G1 exonic regions and exon-intron boundaries of the 29 probands of autosomal dominant, LOPD. Sequence alignment and analysis were carried out with Chromas 2.12 software (Technelysium Pty, Inc., Queensland, Australia).
Additional file 1: Table S1. EIF4G1 Sequencing Primers.
Format: DOCX Size: 18KB Download file
Statistical analysis
Allele and genotype frequencies of PD and control groups were analyzed using the Chi-square test with SPSS 17.0 software (SPSS Inc., Chicago, IL, USA). A two-tailed test p-value of <0.05 was considered statistically significant.
We identified three exonic variants and eight intronic variants across the entire EIF4G1 gene (Table 1). No reported mutations were detected in the EIF4G1 exonic regions in any of the 29 probands. One of the three exonic variants (exon 25, rs2230571) was synonymous, the other two (rs13319149 and rs2178403) were nonsynonymous.
Table 1. EIF4G1 gene variants in 29 probands of LOPD families
Neither the synonymous variant nor the eight intronic variants could be sequenced, but the specific mutation p.R1205H and the two nonsynonymous variants underwent sequencing analysis in the 503 sporadic PD patients and 508 controls. None of the patients or controls carried the specific mutation p.R1205H. The nonsynonymous exonic variant rs2178403 was in Hardy-Weinberg equilibrium in both the patient and control groups using a goodness-of-fit Chi-square test. Genotype and allele frequencies of rs2178403 for all subjects are shown in Table 2. No statistically significant differences were observed regarding rs2178403 genotype or allele frequency between patients and controls (p = 0.184 and p = 0.774, respectively). Regarding the nonsynonymous exonic variant rs13319149, all patient and control genotypes were GG.
Table 2. Genotype and allele frequencies of rs2178403 in PD and controls in Han Chinese
EIF4G1 was confirmed as a candidate PD gene by Chartier-Harlin et al. [14,15], but recently three other groups identified EIF4G1 mutations in both PD patients and healthy controls [21-23]. This raises questions about the causality of the gene with regard to PD. In our comprehensive genetic analysis of EIF4G1, we also obtained similar negative results. The specific mutation p.R1205H, first identified by Chartier-Harlin, is important in PD for regulating cell survival in response to stressors [14,15]. However, it was absent from all of our 503 sporadic PD cases and 508 healthy controls suggesting that different populations may have different pathogenic mutations. Alternatively, the effect of the same mutation may differ in different populations, as indicated by the presence of mutation p.R1205H in control individuals of a Central European population shown by Schulte et al. [23].
In the present study, exonic variants rs13319149 and rs2178403 showed no significant difference in either genotype or allele frequencies between PD patients and controls, suggesting that they are probably benign polymorphisms. Although EIF4G1 is considered a pathogenic gene of LOPD, our 29 probands were not typical of LOPD as the average age of onset was 50.33 ± 9.56 years. This may contribute to our negative results, together with the fact that our sample size was relatively small. Further larger-scale genetic studies should therefore be performed to validate our findings.
To our knowledge, this is the first study to assess the frequency of EIF4G1 variants in a cohort of Chinese PD patients and controls. Our results suggest that in this ethnic Chinese population, the pathogenic mutation p.R1205H in EIF4G1 is not common and that nonsynonymous exonic variants rs2178403 and rs13319149 are not associated with PD. Thus, it appears that EIF4G1 is not a frequent cause of PD in this ethnic Chinese population. Further large scale genetic and functional studies would be useful to establish EIF4G1 associations with PD.
ATP13A2: ATPase type 13A2 gene; df: Degree of freedom; eIF3e: Eukaryotic translation initiation factor 3e; eIF4E: Eukaryotic translation initiation factor 4E; EIF4G1: Eukaryotic translation initiation factor 4-gamma 1 gene; FBXO7: F-box protein 7; LOPD: Late-onset Parkinson disease; LRRK2: Leucine-rich repeat kinase 2 gene; OR: Odds ratio; PCR: Polymerase chain reaction; PD: Parkinson disease; PINK1: PTEN-induced putative kinase 1 gene; PRKN: Parkin; PLA2G6: Phospholipase A2, group VI gene; SNCA: A-synuclein gene; UCHL1: Ubiquitin carboxyl-terminal esterase L1; VPS35: Vacuolar protein sorting 35 gene
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
KL performed the genotyping, statistical analysis, and drafted the manuscript. MXL, ZYL, ZHL, YT, CYS, and KX contributed to the collection of materials, participated in the study design and coordination, and drafted the manuscript. BST, JFG and XXY conceived the study, participated in its conceptual design and coordination, and drafted the manuscript. All authors read and approved the final manuscript.
This work was supported by grants from the Major State Basic Research Development Program of China (973 Program) (2011CB510000); and National Natural Science Foundation of China (81171198, 81130021, 30971035, 30900469, 81000542). We would like to thank all PD patients and health control individuals participating in the study.
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text:
General Sarnoff, the chairman of the board of RCA; Pat Weaver, the president of NBC; Max Liebman and Sid.
But he had to remember one thing: he was promoting himself at this point, Sam Cooke, not RCA.
At the time, the RCA didn't care, and apparently it still doesn't.
1922, initialism of Radio Corporation of America.