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Publications (20)65.09 Total impact
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ABSTRACT: Pathogenicity of the human pathogen Helicobacter pylori relies on its capacity to adapt to a hostile environment and to escape the host response. Although there have been great advances in our understanding of the bacterial cytoskeleton, major gaps remain in our knowledge of its contribution to virulence. In this study we have explored the influence of coiled coil rich proteins (Ccrp) cytoskeletal elements on pathogenicity factors of H. pylori. Deletion of any of the ccrp resulted in a strongly decreased activity of the main pathogenicity factor urease. We further investigated their role using in vitro co-culture experiments with the human gastric adenocarcinoma cell line AGS modeling H. pylori - host cell interactions. Intriguingly, host cell showed only a weak "scattering/hummingbird" phenotype, in which host cells are transformed from a uniform polygonal shape into a severely elongated state characterized by the formation of needle-like projections, after co-incubation with any ccrp deletion mutant. Furthermore, co-incubation with the ccrp59 mutant resulted in reduced type IV secretion system associated activities, e.g. IL-8 production and CagA translocation/phosphorylation. Thus, in addition to their role in maintaining the helical cell shape of H. pylori Ccrp proteins influence many cellular processes and are thereby crucial for the virulence of this human pathogen.PLoS ONE 03/2015; 10(3):e0121463. DOI:10.1371/journal.pone.0121463 · 3.53 Impact Factor
Article: Metallibacterium scheffleri gen. nov., sp. nov., an alkalinizing gammaproteobacterium isolated from an acidic biofilm.[Show abstract] [Hide abstract]
ABSTRACT: The Gram-negative, non-motile, rod shaped, facultative anaerobic and acid tolerant bacterial strain DKE6(T) was isolated from an acidic biofilm (pH 2.5) that was harvested in the pyrite mine 'Drei Kronen und Ehrt' in Germany. The strain grows optimally at pH 5.5, between 25°C and 30°C and with casein as carbon and energy source. Although a variety of sugars was tested as growth substrates, none of these supported growth of the strain. During casein consumption the DKE6(T) produces ammonium which leads to an alkalization of the medium. This is a possible strategy to raise the pH in direct vicinity of the cell and hence modulate the pH towards the growth optimum. The predominant fatty acids are iso-C(11:0) 3-OH, anteiso-C(15:0), iso-C(17:0) and iso-C(17:1) ω9c. The DNA G+C content is 66.6%. Strain DKE6(T) is not able to oxidize iron or thiosulfate. Iron reduction was detected. The strain shows 93.3% 16S rRNA gene sequence similarity to the closest related cultivated species Dokdonella koreensis and 93.2% or less to other type species of the Gammaproteobacteria. On the basis of physiological and biochemical data, the new strain is considered to represent a member of a novel genus in the class of the Gammaproteobacteria. We propose the name Metallibacterium scheffleri gen. nov., sp. nov., for the new isolate. The type strain is DKE6(T) (DSM 24874(T); JCM 17596(T)).International Journal of Systematic and Evolutionary Microbiology 04/2013; 63(4):1499-1504. DOI:10.1099/ijs.0.042986-0 · 2.80 Impact Factor
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ABSTRACT: Of the various kinds of cell division, the most common mode is binary fission, the division of a cell into two morphological identical daughter cells. However in the case of asymmetric cell division, C. crescentus produces two morphological and functional distinct cell types. Here, we have studied cell cycle progression of the human pathogen Helicobacter pylori using a functional GFP-fusion of FtsZ protein and membrane staining. In small cells, representing newly divided cells, FtsZ localizes to a single cell pole. During the cell cycle, spiral intermediates are formed, until a FtsZ-ring is positioned with little precision, such that central as well as acentral rings can be observed. Daughter cells showed considerably different sizes, suggesting that H. pylori divides asymmetrically. FRAP analyses demonstrate that the H. pylori FtsZ-ring is about as dynamic as that of E. coli but that polar assemblies show less turnover. Strikingly our results demonstrate that H. pylori cell division follows a different route from that in E. coli and B. subtilis. It is also different from that in C. crescentus, where cytokinesis regulation proteins like MipZ play a role. Therefore this report provides the first cell biological analysis of FtsZ dynamics in the human pathogen H. pylori and even in ε-proteobacteria to our knowledge. In addition, analysis of the filament architecture of H. pylori and E. coli FtsZ filaments in the heterologous system of D. melanogaster S2 Schneider cells revealed that both have different filamentation properties in vivo suggesting a unique intrinsic characteristic of each protein.Journal of bacteriology 01/2013; DOI:10.1128/JB.01490-12 · 2.69 Impact Factor
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ABSTRACT: Helicobacter pylori colonizes the stomachs of at least half of the world's human population. The role of the oral cavity in this colonization is not clear and there are, to date, no comprehensive data that clearly demonstrate the isolation of this bacterium from the oral cavity. The aim of this study was to evaluate the prevalence of H. pylori in the oral cavity of 15 patients who tested positive for H. pylori. A comprehensive dental examination of all patients was conducted. Samples were taken from supragingival and subgingival plaque, saliva, periapical exudates and tongue swabs. All samples were taken before the application of antibiotics. A total of 163 oral samples were investigated by PCR using two different H. pylori-specific primer pairs. A PCR inhibition control using a modified plasmid was always included for the most specific primer pair. In addition, a culture technique was used to confirm PCR results. Despite a PCR detection limit of 10(2) bacteria ml(-1), out of 14 patients, H. pylori could not be detected in any of the samples taken. In one patient, H. pylori-positive PCR signals were obtained in two samples using only one primer pair. H. pylori could not be cultivated from these two PCR-positive samples; therefore, no correlation to oral colonization status could be established. This study challenges the misleading preconception that H. pylori resides in the human oral cavity and suggests that this bacterium should be considered transient and independent of the oral status. To date, positive PCR results for H. pylori in the oral cavity have been overestimated and not critically interpreted in literature.Journal of Medical Microbiology 04/2012; 61(Pt 8):1146-52. DOI:10.1099/jmm.0.043893-0 · 2.27 Impact Factor
Article: Helicobacter pylori Possesses Four Coiled-Coil-Rich Proteins That Form Extended Filamentous Structures and Control Cell Shape and Motility[Show abstract] [Hide abstract]
ABSTRACT: We identified two additional genes of Helicobacter pylori encoding Ccrp proteins. All four Ccrps have different multimerization and filamentation properties and different types of smallest subunits and do not copurify, suggesting a system of individual Ccrp filaments. Despite the presence of morphologically unaltered flagella, all ccrp mutants displayed significantly reduced motility.Journal of bacteriology 06/2011; 193(17):4523-30. DOI:10.1128/JB.00231-11 · 2.69 Impact Factor
Article: The putative assembly factor CcoH is stably associated with the cbb3-type cytochrome oxidase.[Show abstract] [Hide abstract]
ABSTRACT: Cytochrome oxidases are perfect model substrates for analyzing the assembly of multisubunit complexes because the need for cofactor incorporation adds an additional level of complexity to their assembly. cbb(3)-type cytochrome c oxidases (cbb(3)-Cox) consist of the catalytic subunit CcoN, the membrane-bound c-type cytochrome subunits CcoO and CcoP, and the CcoQ subunit, which is required for cbb(3)-Cox stability. Biogenesis of cbb(3)-Cox proceeds via CcoQP and CcoNO subcomplexes, which assemble into the active cbb(3)-Cox. Most bacteria expressing cbb(3)-Cox also contain the ccoGHIS genes, which encode putative cbb(3)-Cox assembly factors. Their exact function, however, has remained unknown. Here we analyzed the role of CcoH in cbb(3)-Cox assembly and showed that CcoH is a single spanning-membrane protein with an N-terminus-out-C-terminus-in (N(out)-C(in)) topology. In its absence, neither the fully assembled cbb(3)-Cox nor the CcoQP or CcoNO subcomplex was detectable. By chemical cross-linking, we demonstrated that CcoH binds primarily via its transmembrane domain to the CcoP subunit of cbb(3)-Cox. A second hydrophobic stretch, which is located at the C terminus of CcoH, appears not to be required for contacting CcoP, but deleting it prevents the formation of the active cbb(3)-Cox. This suggests that the second hydrophobic domain is required for merging the CcoNO and CcoPQ subcomplexes into the active cbb(3)-Cox. Surprisingly, CcoH does not seem to interact only transiently with the cbb(3)-Cox but appears to stay tightly associated with the active, fully assembled complex. Thus, CcoH behaves more like a bona fide subunit of the cbb(3)-Cox than an assembly factor per se.Journal of bacteriology 10/2010; 192(24):6378-89. DOI:10.1128/JB.00988-10 · 2.69 Impact Factor
Article: Re: The Impact of the Stone Age Diet on Gingival Conditions in the Absence of Oral Hygiene. Baumgartner S, Imfeld T, Schicht O, Rath C, Persson RE, Persson GR. (J Periodontol 2009;80:759-768.)Journal of Periodontology 03/2010; 81(3):338; author reply 338-9. DOI:10.1902/jop.2010.090546 · 2.57 Impact Factor
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ABSTRACT: Author Summary The human pathogen Helicobacter pylori lives in the hostile environment of the human stomach. H. pylori possesses a spiral shape and high motility that enable the bacterium to swim through the stomach lumen and to come into close contact with epithelial cells. High urease activity in the bacterium counterbalances the low pH within the stomach, in order to persist within the viscous mucus layer. In this work, we analysed the molecular basis of the spiral structure of H. pylori. We demonstrate that the helical cell shape depends on so called coiled coil rich proteins (Ccrp), which form extended filamentous structures in vitro and in vivo, and are differentially required for the maintenance of proper cell morphology. In most bacteria analysed so far, the actin-like protein MreB affects cell morphology. Contrarily, H. pylori MreB is not involved in the maintenance of cell shape, but affects the progression of the cell cycle. Mutant cells were highly elongated, characteristic for a delay in cell division, and contained non-segregated chromosomes. The persistence of H. pylori in the hostile environment of the human stomach depends on the activity of urease. Interestingly, mreB mutant cells displayed significantly reduced urease activity, revealing a novel connection between the cytoskeletal element and an enzyme, and thus with pathogenicity. These experiments show that H. pylori has a novel type of system setting up helical cell shape, which has not yet been described for any bacterium. Our work will allow studying H. pylori cell cycle and pathogenicity at a new visual level.PLoS Pathogens 11/2009; 5(11):e1000669. DOI:10.1371/journal.ppat.1000669 · 8.06 Impact Factor
Article: The Helicobacter pylori CrdRS two-component regulation system (HP1364/HP1365) is required for copper-mediated induction of the copper resistance determinant CrdA.[Show abstract] [Hide abstract]
ABSTRACT: Here we describe that the Helicobacter pylori sensor kinase produced by HP1364 and the response regulator produced by HP1365 and designated CrdS and CrdR, respectively, are both required for transcriptional induction of the H. pylori copper resistance determinant CrdA by copper ions. CrdRS-deficient mutants lacked copper induction of crdA expression and were copper sensitive. A direct role of CrdR in transcriptional regulation of crdA was confirmed by in vitro binding of CrdR to the crdA upstream region. A 21-nucleotide sequence located near the crdA promoter was shown to be required for CrdR binding.Journal of Bacteriology 08/2005; 187(13):4683-8. DOI:10.1128/JB.187.13.4683-4688.2005 · 2.69 Impact Factor
Article: Iron-responsive regulation of the Helicobacter pylori iron-cofactored superoxide dismutase SodB is mediated by Fur.[Show abstract] [Hide abstract]
ABSTRACT: Maintaining iron homeostasis is a necessity for all living organisms, as free iron augments the generation of reactive oxygen species like superoxide anions, at the risk of subsequent lethal cellular damage. The iron-responsive regulator Fur controls iron metabolism in many bacteria, including the important human pathogen Helicobacter pylori, and thus is directly or indirectly involved in regulation of oxidative stress defense. Here we demonstrate that Fur is a direct regulator of the H. pylori iron-cofactored superoxide dismutase SodB, which is essential for the defense against toxic superoxide radicals. Transcription of the sodB gene was iron induced in H. pylori wild-type strain 26695, resulting in expression of the SodB protein in iron-replete conditions but an absence of expression in iron-restricted conditions. Mutation of the fur gene resulted in constitutive, iron-independent expression of SodB. Recombinant H. pylori Fur protein bound with low affinity to the sodB promoter region, but addition of the iron substitute Mn2+ abolished binding. The operator sequence of the iron-free form of Fur, as identified by DNase I footprinting, was located directly upstream of the sodB gene at positions -5 to -47 from the transcription start site. The direct role of Fur in regulation of the H. pylori sodB gene contrasts with the small-RNA-mediated sodB regulation observed in Escherichia coli. In conclusion, H. pylori Fur is a versatile regulator involved in many pathways essential for gastric colonization, including superoxide stress defense.Journal of Bacteriology 07/2005; 187(11):3687-92. DOI:10.1128/JB.187.11.3687-3692.2005 · 2.69 Impact Factor
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ABSTRACT: Intracellular iron homeostasis is a necessity for almost all living organisms, since both iron restriction and iron overload can result in cell death. The ferric uptake regulator protein, Fur, controls iron homeostasis in most Gram-negative bacteria. In the human gastric pathogen Helicobacter pylori, Fur is thought to have acquired extra functions to compensate for the relative paucity of regulatory genes. To identify H. pylori genes regulated by iron and Fur, we used DNA array-based transcriptional profiling with RNA isolated from H. pylori 26695 wild-type and fur mutant cells grown in iron-restricted and iron-replete conditions. Sixteen genes encoding proteins involved in metal metabolism, nitrogen metabolism, motility, cell wall synthesis and cofactor synthesis displayed iron-dependent Fur-repressed expression. Conversely, 16 genes encoding proteins involved in iron storage, respiration, energy metabolism, chemotaxis, and oxygen scavenging displayed iron-induced Fur-dependent expression. Several Fur-regulated genes have been previously shown to be essential for acid resistance or gastric colonization in animal models, such as those encoding the hydrogenase and superoxide dismutase enzymes. Overall, there was a partial overlap between the sets of genes regulated by Fur and those previously identified as growth-phase, iron or acid regulated. Regulatory patterns were confirmed for five selected genes using Northern hybridization. In conclusion, H. pylori Fur is a versatile regulator involved in many pathways essential for gastric colonization. These findings further delineate the central role of Fur in regulating the unique capacity of H. pylori to colonize the human stomach.Microbiology 03/2005; 151(Pt 2):533-46. DOI:10.1099/mic.0.27404-0 · 2.84 Impact Factor
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ABSTRACT: Research in the last year has provided new insights into the function of the the cag-associated type IV secretion system and the vacuolating toxin VacA. A quite new aspect was disclosed by the finding that Helicobacter pylori in Mongolian gerbils colonizes a very distinct topology in the gastric mucous layer, obviously providing optimal conditions for long-term survival. Further research activities focused on H. pylori ammonia and metal metabolism as well as on bacterial stress defence mechanisms. Differential expression of approximately 7% of the bacterial genome was found at low pH suggesting that H. pylori has evolved a multitude of acid-adaptive mechanisms. VacA was shown to interrupt phagosome maturation in macrophage cell lines as well as to modulate and interfere with T lymphocyte immunological functions. Gastric mucosa as well as the H. pylori-infected epithelial cell line AGS strongly express IL-8 receptor A and B, which might contribute to the augmentation of the inflammatory response. Accumulating evidence implicates genetic variation in the inflammatory response to H. pylori in the etiology of the increased risk of gastric cancer after H. pylori infection. The chronic imbalance between apoptosis and cell proliferation is the first step of gastric carcinogenesis. In this regard, it was demonstrated that coexpression of two H. pylori proteins, CagA and HspB, in AGS cells, caused an increase in E2F transcription factor, cyclin D3, and phosphorylated retinoblastoma protein. Taken together, we now have a better understanding of the role of different virulence factors of H. pylori. There is still a lot to be learned, but the promising discoveries summarized here, demonstrate that the investigation of the bacterial survival strategies will give novel insights into pathogenesis and disease development.Helicobacter 02/2004; 9 Suppl 1:15-22. DOI:10.1111/j.1083-4389.2004.00246.x · 2.99 Impact Factor
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ABSTRACT: Poxviruses have evolved various strategies to counteract the host immune response, one of which is based on the expression of soluble cytokine receptors. Using various biological assays, we detected a chicken interferon-gamma (chIFN-gamma)-neutralizing activity in supernatants of fowlpox virus (FPV)-infected cells that could be destroyed by trypsin treatment. Secreted viral proteins were purified by affinity chromatography using matrix-immobilized chIFN-gamma, followed by two-dimensional gel electrophoresis. Matrix-assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) analysis indicated that the viral IFN-gamma-binding protein in question was encoded by the FPV gene 016. The chicken IFN-gamma binding and neutralizing activity of the recombinant FPV016 protein was confirmed using supernatants of cells infected with a recombinant vaccinia virus that lacked its own IFN-gamma-binding protein but instead expressed the FPV016 gene. The FPV016 gene product also neutralized the activity of duck and human IFN-gamma but failed to neutralize the activity of mouse and rat IFN-gamma. Unlike previously known cellular and poxviral IFN-gamma receptors, which all contain fibronectin type III domains, the IFN-gamma-binding protein of FPV contains an immunoglobulin domain. Remarkably, it exhibits no significant homology to any known viral or cellular protein. Because IFN-gamma receptors of birds have not yet been characterized at the molecular level, the possibility remains that FPV016 represents a hijacked chicken gene and that avian and mammalian IFN-gamma receptors have fundamentally different primary structures.Journal of Biological Chemistry 03/2003; 278(9):6905-11. DOI:10.1074/jbc.M207336200 · 4.60 Impact Factor
Article: Identification by RNA Profiling and Mutational Analysis of the Novel Copper Resistance Determinants CrdA (HP1326), CrdB (HP1327), and CzcB (HP1328) in Helicobacter pylori[Show abstract] [Hide abstract]
ABSTRACT: Mechanisms involved in maintaining cytoplasmic metal ion homeostasis play a central role in the adaptation of Helicobacter pylori to the changing gastric environment. An investigation of the global regulatory responses to copper ions by using RNA profiling with a threshold factor of 4.0 revealed that copper induces transcription of 19 H. pylori genes and that only the ferritin gene pfr is repressed. The 57-fold copper induction identified the HP1326 gene encoding an H. pylori-specific protein as a candidate for a novel copper resistance determinant. The HP1326 gene is expressed as a monocistronic unit, and two small HP1326 mRNAs are copper induced. The HP1326 protein is secreted and is required for copper resistance maintained by cytoplasmic copper homeostasis, as H. pylori HP1326 mutants were copper sensitive and displayed increased copper induction of HP1326 transcription as well as elevated copper repression of ferritin synthesis. The clear copper-sensitive phenotype displayed by H. pylori HP1327 and HP1328 mutants provides strong evidence that the HP1326 protein, together with the signal peptide site of the H. pylori-specific protein HP1327, whose gene is located downstream from that encoding HP1326, and the CzcB and CzcA metal efflux system component homologs HP1328 and HP1329, constitutes a novel type of copper efflux pump, as discussed below. The HP1329 gene could not be inactivated, but the 14-fold transcriptional copper induction determined by RNA profiling points towards a function of the encoded CzcA homolog in copper resistance. In summary, results from RNA profiling identified the novel H. pylori-specific copper resistance determinants CrdA (HP1326) and CrdB (HP1327), which are required for adaptation to copper-rich environmental conditions.Journal of Bacteriology 01/2003; 184(23):6700-8. DOI:10.1128/JB.184.23.6700-6708.2002 · 2.69 Impact Factor
Article: Magnesium uptake by CorA is essential for viability of the gastric pathogen Helicobacter pylori.[Show abstract] [Hide abstract]
ABSTRACT: We show here that Mg(2+) acquisition by CorA is essential for Helicobacter pylori in vitro, as corA mutants did not grow in media without Mg(2+) supplementation. Complementation analysis performed with an Escherichia coli corA mutant revealed that H. pylori CorA transports nickel and cobalt in addition to Mg(2+). However, Mg(2+) is the dominant CorA substrate, as the corA mutation affected neither cobalt and nickel resistance nor nickel induction of urease in H. pylori. The drastic Mg(2+) requirement (20 mM) of H. pylori corA mutants indicates that CorA plays a key role in the adaptation to the low-Mg(2+) conditions predominant in the gastric environment.Infection and Immunity 08/2002; 70(7):3930-4. DOI:10.1128/IAI.70.7.3930-3934.2002 · 4.16 Impact Factor
Article: Essential role of ferritin Pfr in Helicobacter pylori iron metabolism and gastric colonization.[Show abstract] [Hide abstract]
ABSTRACT: The reactivity of the essential element iron necessitates a concerted expression of ferritins, which mediate iron storage in a nonreactive state. Here we have further established the role of the Helicobacter pylori ferritin Pfr in iron metabolism and gastric colonization. Iron stored in Pfr enabled H. pylori to multiply under severe iron starvation and protected the bacteria from acid-amplified iron toxicity, as inactivation of the pfr gene restricted growth of H. pylori under these conditions. The lowered total iron content in the pfr mutant, which is probably caused by decreased iron uptake rates, was also reflected by an increased resistance to superoxide stress. Iron induction of Pfr synthesis was clearly diminished in an H. pylori feoB mutant, which lacked high-affinity ferrous iron transport, confirming that Pfr expression is mediated by changes in the cytoplasmic iron pool and not by extracellular iron. This is well in agreement with the recent discovery that iron induces Pfr synthesis by abolishing Fur-mediated repression of pfr transcription, which was further confirmed here by the observation that iron inhibited the in vitro binding of recombinant H. pylori Fur to the pfr promoter region. The functions of H. pylori Pfr in iron metabolism are essential for survival in the gastric mucosa, as the pfr mutant was unable to colonize in a Mongolian gerbil-based animal model. In summary, the pfr phenotypes observed give new insights into prokaryotic ferritin functions and indicate that iron storage and homeostasis are of extraordinary importance for H. pylori to survive in its hostile natural environment.Infection and Immunity 08/2002; 70(7):3923-9. DOI:10.1128/IAI.70.7.3923-3929.2002 · 4.16 Impact Factor
Article: The Helicobacter pylori homologue of the ferric uptake regulator is involved in acid resistance.[Show abstract] [Hide abstract]
ABSTRACT: The only known niche of the human pathogen Helicobacter pylori is the gastric mucosa, where large fluctuations of pH occur, indicating that the bacterial response and resistance to acid are important for successful colonization. One of the few regulatory proteins in the H. pylori genome is a homologue of the ferric uptake regulator (Fur). In most bacteria, the main function of Fur is the regulation of iron homeostasis. However, in Salmonella enterica serovar Typhimurium, Fur also plays an important role in acid resistance. In this study, we determined the role of the H. pylori Fur homologue in acid resistance. Isogenic fur mutants were generated in three H. pylori strains (1061, 26695, and NCTC 11638). At pH 7 there was no difference between the growth rates of mutants and the parent strains. Under acidic conditions, growth of the fur mutants was severely impaired. No differences were observed between the survival of the fur mutant and parent strain 1061 after acid shock. Addition of extra iron or removal of iron from the growth medium did not improve the growth of the fur mutant at acidic pH. This indicates that the phenotype of the fur mutant at low pH was not due to increased iron sensitivity. Transcription of fur was repressed in response to low pH. From this we conclude that Fur is involved in the growth at acidic pH of H. pylori; as such, it is the first regulatory protein implicated in the acid resistance of this important human pathogen.Infection and Immunity 03/2002; 70(2):606-11. DOI:10.1128/IAI.70.2.606-611.2002 · 4.16 Impact Factor
Article: Nickel-responsive induction of urease expression in Helicobacter pylori is mediated at the transcriptional level.[Show abstract] [Hide abstract]
ABSTRACT: The nickel-containing enzyme urease is an essential colonization factor of the gastric pathogen Helicobacter pylori, as it allows the bacterium to survive the acidic conditions in the gastric mucosa. Although urease can represents up to 10% of the total protein content of H. pylori, expression of urease genes is thought to be constitutive. Here it is demonstrated that H. pylori regulates the expression and activity of its urease enzyme as a function of the availability of the cofactor nickel. Supplementation of brucella growth medium with 1 or 100 microM NiCl(2) resulted in up to 3.5-fold-increased expression of the urease subunit proteins UreA and UreB and up to 12-fold-increased urease enzyme activity. The induction was specific for nickel, since the addition of cadmium, cobalt, copper, iron, manganese, or zinc did not affect the expression of urease. Both Northern hybridization studies and a transcriptional ureA::lacZ fusion demonstrated that the observed nickel-responsive regulation of urease is mediated at the transcriptional level. Mutation of the HP1027 gene, encoding the ferric uptake regulator (Fur), did not affect the expression of urease in unsupplemented medium but reduced the nickel induction of urease expression to only twofold. This indicates that Fur is involved in the modulation of urease expression in response to nickel. These data demonstrate nickel-responsive regulation of H. pylori urease, a phenomenon likely to be of importance during the colonization and persistence of H. pylori in the gastric mucosa.Infection and Immunity 09/2001; 69(8):4891-7. DOI:10.1128/IAI.69.8.4891-4897.2001 · 4.16 Impact Factor
Article: Regulation of ferritin-mediated cytoplasmic iron storage by the ferric uptake regulator homolog (Fur) of Helicobacter pylori.[Show abstract] [Hide abstract]
ABSTRACT: Homologs of the ferric uptake regulator Fur and the iron storage protein ferritin play a central role in maintaining iron homeostasis in bacteria. The gastric pathogen Helicobacter pylori contains an iron-induced prokaryotic ferritin (Pfr) which has been shown to be involved in protection against metal toxicity and a Fur homolog which has not been functionally characterized in H. pylori. Analysis of an isogenic fur-negative mutant revealed that H. pylori Fur is required for metal-dependent regulation of ferritin. Iron starvation, as well as medium supplementation with nickel, zinc, copper, and manganese at nontoxic concentrations, repressed synthesis of ferritin in the wild-type strain but not in the H. pylori fur mutant. Fur-mediated regulation of ferritin synthesis occurs at the mRNA level. With respect to the regulation of ferritin expression, Fur behaves like a global metal-dependent repressor which is activated under iron-restricted conditions but also responds to different metals. Downregulation of ferritin expression by Fur might secure the availability of free iron in the cytoplasm, especially if iron is scarce or titrated out by other metals.Journal of Bacteriology 12/2000; 182(21):5948-53. DOI:10.1128/JB.182.21.5948-5953.2000 · 2.69 Impact Factor
Article: Identifikation neuer Mechanismen der Metallhomöostase des Magenbakteriums Helicobacter pylori /[Show abstract] [Hide abstract]
ABSTRACT: Karlsruhe, Univ., Diss., 2003 (Nicht für den Austausch).
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University of Freiburg
Freiburg, Baden-Württemberg, Germany
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Freiburg an der Elbe, Lower Saxony, Germany
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I hope everyone's doing great. I love you all!!
I hope everyone's doing great. I love you all!!
Everything below is the exact words from sonic_rusher.
What if gamefreak and Nintendo both made an MMORPG for pokemon. It would be awesome. Instead of turned-based battle, you have a set of moves for every pokemon that can be selected at the bottom screen. When you have a flying pokemon with you, you can actually fly your trainer to places. Each pokemon could have 8 moves each that you can quickly choose from your attack menu at the bottom of the screen.
You should be able to design your own character. When you have a pokemon out, you can move around with it, dodge with it, and jump with it. You will see pokemon free roaming that you can catch. Pokemon are placed in certain areas depending on their type. Of course, you will go around to get all eight badges. You can also make your own gym and design your badges once you become a GM.
It could be called POKEMON REBORN. Gamefreak and nintendo, this idea is 100% free to use, you have my full permission.
If you like the idea, spread the word by posting it as your latest blog.*
Once it gets big enough, they might actually make the game for Wii*
Again, this is not my idea it is sonic_rusher's. I got all this here.
For those who are slow and do not know what i am getting hint:*WIINNER* I am getting the Wii!!!!!! I am soooooo excited!!!!!!!
I am struggling right now. I am in a conflict in whether I should have Wii or PS3.
Wii has the controller, and that caught my eye. It also has the newest and (most likely) the best zelda game yet. Wii has other good games like Super Mario Galaxy, Red Steel, Rayman, Elebits and more.
PS3 on the other hand has really good grahics. Sony has Metal Gear Solid series, Final Fantasy series. PS3 will have Resistance and Motorstorm.
I am deciding between Wii and PS3. Stay tuned because soon enough I will have the answer!
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The Returning Officer: Abingdon I
Returning Officer
Returning Officer
by Stephen Brasher Published
In 1921, an anonymous donor paid to have a catalogue of Allied war poetry made for Birmingham public libraries. It was dedicated to Private William John Billington of the London Regiment, who had been killed in Palestine in March 1918. One of the volumes listed was D F (Daniel Fernley) Brundrit's Gleanings, published in 1919.
In 1924, Brundrit stood as the Labour candidate for Abingdon, finishing third. He is listed as having commanded a tank called Cynic in 1917, being taken prisoner in November that year. In 1934, he published Is the Resurrection True? – which the Tablet said could "hardly be considered a serious contribution to the subject".


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Facilities try out their green thumbPublished in SportsBusiness Journal on 06 / 29 / 15
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Players set to cash in making appearances at the Super Bowl
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On the brink?
The NFL has for more than a decade now been America's powerhouse sport, known for its popularity and staggering revenue growth. Underpinning all of that has been labor peace. But as the league prepares for its showcase game Sunday, the sport's much-admired relations with its union ar ...
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Arena Football League: A history of playing rough indoors
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Turnkey Sports Poll
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#545 of 567
New Orleans, LA
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Faded Glory
Cheri Randolph
This vintage private rail car was photographed in the Galveston Railroad Museum (Texas). It shows a considerable amount of weathering, but with only a little imagination, one can imagine the luxury it provided in the heyday of rail travel in this country.
A private railroad car, private railway coach, private car or private varnish is a railroad passenger car which was either originally built or later converted for service as a business car for private individuals. A private car could be added to the make-up of a train or pulled by a private locomotive, providing splendid upholstered privacy for its passengers.
They were used by railroad officials and dignitaries as business cars, and wealthy individuals for travel and entertainment, especially in the United States. They were sometimes used by politicians in "whistle stop campaigns". Pay cars with less opulent sleeping and dining facilities were used by a paymaster and assistants to transport and disburse cash wages to railway employees in remote locations without banking facilities.
In the late 19th century Gilded Age, wealthy individuals had finely appointed private cars custom-built to their specifications. Additionally many cars built by Pullman, Budd, and other companies that were originally used in common carrier service as passenger cars were later converted for use as business and private cars. There are various configurations, but the cars generally have an observation platform, a full kitchen, dining room, state rooms, secretary's room, an observation room, and often servant's quarters.
Railroad barons including Leland Stanford had their private cars. Abraham Lincoln disliked the ornate railroad car supplied for his service as president: he rode in it only in his coffin. Private cars were more common in the heyday of passenger rail service and during the pre-Amtrak era (before 1971). In modern times, some private cars have survived the decades and some are used for tour rides, leasing for private events, etc. Others are on static display. A small number of private cars (along with other types of passenger cars), have been upgraded to meet current Amtrak regulations, and may be chartered by their owners for private travel attached to Amtrak trains.
Note: This image has been processed using a digital watercolor filter. It will be more effectively printed on a matte surface paper such as watercolor paper or Somerset Velvet.
February 3rd, 2012
Viewed 169 Times - Last Visitor from New York, NY on 07/07/2015 at 4:14 AM
Sales Sheet
More from Cheri Randolph
Colorado &, N. - United States
Cheri, this is one of my fav's of your work, which is all great....but this one is like something out of an old western movie! voted!
Barbara, Thanks so much for commenting and voting for this one. I enjoy railroad photographs, too, there is such a romance about them.
Kerrville, TX - United States
Beautiful image...the WC filter was a good choice. Were you able to get inside?
Thanks, Jake! Yes, the watercolor look seemed to be just right for the rusted textures of the metal. You can go inside many of them, but this one was locked.
Dallas, TX - United States
They don't make things like this anymore, beautiful metal work and patina of time - real treasure! v/f
Elena, I agree. It must have been very luxurious in its day! Appreciate your v/f!
Hayden, ID - United States
Gritty, forlorn and gorgeous to boot. Great job Cheri! f/v
Doug, that's what I was thinking as well! Thanks for visiting and your f/v!
New Orleans, LA - United States
Nadine & Bob, Thanks so much for featuring "Faded Glory" in the USA Artist News. I am very impressed with the response of your readership. Cheri
Houston, TX - United States
Gorgeous! v/f
Tessa, Thanks for visiting! Appreciate your v/f!
Lebanon, OR - United States
I love this composition Cheri and the detail you captured is amazing.. F/V
Nick, Thanks. It was a very interesting rail car. Appreciate your F/V!
Pe�aflor - Chile
CONGRATULATIONS!! Featured on Female Artists!! Great work, love how you have done this!!! Very well done!!
Madalena, I'm honored to be featured in your group of creative women artists. Thanks!!!
Mesa, AZ - United States
I love this shot. All the patterns and textures and tones of color. I voted/pinit
Marcia, I am complimented by your favorable critique. Appreciate your generous support!
Daytona Beach, FL - United States
Wonderful atmosphere!! f/v
Deborah, Thanks for your comment and the F/V!
Lockhart, TX - United States
I Love this! v/f
Kelly, I'm glad you like it. Appreciate the V/F!
Greensboro, NC - United States
Great find, comp, love your processing, excellent Cheri, f/v.
Sandi, Thanks for your compliment and such nice support!
New Orleans, LA - United States
Jose, I appreciate your feature of "Faded Glory" in the Stop Time with Art Group.
Dent, MN - United States
Love this, Cheri, nicely composed! V
Penny, Many thanks for commenting and voting!
New Orleans, LA - United States
Nadine & Bob, Thanks! Your feature of "Faded Glory" in the Beauty of this World Group is much appreciated.
Hermann, MO - United States
Back to vote and favorite. Still loving this picture.
William, Thanks for visiting. I'm complimented that you would list this as a favorite.
Presque Isle, ME - United States
Excellent Cheri! f/v
Gary, Thanks! I appreciate your generous support.
Fort Worth, TX - United States
wonderful old rail car! v
Joan, Thanks for your compliment & vote.
Sandy Hook, KY - United States
Neat shot love it V
Sandra, Thanks! I appreciate your vote.
New Orleans, LA - United States
Bruce, Many thanks for featuring "Faded Glory" in the Comfortable Art Group!
Fruita, CO - United States
v8, well done, the lighting is great.
Bob, Thanks! Appreciate your compliment and the vote, too.
Mequon, WI - United States
Wonderful capture and perfect processing to create a nostalgic feel. vf
Mary, Thanks for your nice compliment and the v/f, too!
Iola, WI - United States
Unique antiquated look to this entrance and the railing design is neat along with the door, Cheri! fave and vote
Randy, I'm glad that you enjoyed this one
Fort Worth, TX - United States
love old abandoned rusted things! v
Joan, Thanks. Me, too. Appreciate your vote!
Wheaton, IL - United States
I love the coloring on this image and how it blends with the rust on the iron railings! vtd
Rosanne, Thanks. I think that the light was just right for it to happen. Appreciate your vote.
New Orleans, LA - United States
Lois, I'm glad that you appreciated this one. I was surprised myself at the elegance of this railcar. It was certainly the equivalent of "First Class" back in the day. Thanks for your comment.
Brookeville, MD - United States
What a wonderful find ... I love the ornate iron work and the faded but still beautiful wooden door and windows. Love the tones and your editing is gorgeous!!!
Hermann, MO - United States
Cheri, this is a treasure!
William, Thanks for your support. I have a special fondness for vintage Railroad cars. Cheri
Please Wait...
Beverly Hills, CA (United States)
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I think if she was allowed, she'd already be riding rollercoasters and bungie jumping! Is this normal??? Of course, I'm still very careful given she's only 5 1/2 months old, but she bust up laughing everytime I pretend I'm going to drop her, lift her in the air over and over, spin around, bounce her, whatever. It worries me that I'm going to hurt her or rattle her brain or something, but she loves it so much.
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Oh and now she's doing this thing where she will randomly, out of the blue - throws herself backward while your holding her! It was so scary the first time she did it cause I wasn't expecting that. I think she does it when she wants to play.
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He also thinks it's hilarious when I "eat" his elbows lol
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That's so cute, Soitgoes! Mine laughs when I "eat" her feet, chew them up and spit them out. She cracks up!
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North Bay at the Lakes
- Work 1-612-345-8301
- Blaine, MN
- 45.1948 -93.1887
Huntington Place
- Work 1-612-440-3308
- Brooklyn Park, MN
- 45.0872621 -93.3520466
- Work 1-877-452-0185
- Elk River, MN
- 45.304753929377 -93.559312149882
152 Apartments for Rent in 55304
Of the 152 apartments in the Andover area, we found exactly 1 apartments for rent in the 55304 zip code of Andover, Minnesota and 151 apartments nearby. Refine your search by using the filter at the top of the page to view 1, 2 or 3+ bedroom apartments for rent in 55304 as well as cheap apartments, pet friendly apartments, apartments with utilities included and more. Use our customizable guide to refine your options for apartments in the 55304 zip code and find exactly what you want during your Andover, Minnesota apartment search.
North Bay at the Lakes3195 124th Avenue NE Blaine, MN 5544945.1948 -93.1887- Near 55304+−Preview DetailsCollapse Details
North Bay at the Lakes has brand new 1, 2, and 3 bedroom townhomes for rent in Blaine, MN. Townhouses have an attached garage, private patio, private entrance, 9-ft ceilings, in-home washer and dryer, and a modern kitchens with a dishwasher and microwave. Amenities include a fitness room and clubhouse with wi-fi and a patio. North Bay is one block from a new swimming beach with boat rentals, concession stand, volleyball courts, and a play area for kids. Enjoy 15 miles of walking/biking trails.
- Brand New Townhomes with 1, 2, and 3 Bedrooms
- In-Home Full Size Washer and Dryer
- Attached Private Garage with Opener
- Fitness Center
- Spacious Private Patio
- Stainless Appliances: Dishwasher and Microwave
- Clubhouse
- Private Entrance
- Convenient Highway Access to 35W and Cty Rd. 65
- Several Miles of Walking/Biking Trails Nearby
- Walking Distance to Swimming Beach
- Minutes to Shopping, Restaurants, and Groceries
Greens at Edinburgh8617 Edinbrook Crossing Brooklyn Park, MN 5544345.109206 -93.328574- Near 55304+−Preview DetailsCollapse Details
The Greens at Edinburgh has studio, 1, 2, and 3 bedroom apartments in Brooklyn Park on golf course. Apartments have full size washer and dryer, private patio/balcony, kitchen with dishwasher & microwave, central air conditioning, and oversize closets. Community amenities include underground parking, swimming pool, fitness center, tanning salon, hot tub, club room, and guest suite. Near highways 252, 610, and I-694. Cats & dogs welcome. 15 minutes to downtown Minneapolis.
- Underground Heated Garage Parking
- Full-Size Washer & Dryer in Units
- 24-Hour Fitness Center
- Outdoor Pool, Sundeck and Tanning Room
- Near Highways 252, 694, and 610
- Fireplaces in Select Homes
- Cats and Small Dogs are Welcome
- Golfer's Lounge and Clubhouse
- Golf Course Discount for Residents
- Gourmet Eat-In Kitchens
- High-Speed Internet Access
- Make Payments Online
Skye at Arbor Lakes11851 Central Parkway Maple Grove, MN 5536945.098289489746 -93.443099975586- Near 55304+−Preview DetailsCollapse Details
Brand new modern 1 and 2 bedroom apartment homes for rent in Maple Grove, MN next to The Shoppes at Arbor Lakes shopping area. Homes include kitchen islands, granite counter tops, stainless steel appliances, full size washer and dryer, patios or balconies, and faux wood flooring. Community has clubhouse, fitness center, spa, pool, yoga room, and more. Pet friendly and features outdoor dog park.
- Fully Equipped Gourmet Kitchens w/ Islands
- Heated Underground Parking Included
- Pet Friendly- Large Dogs Welcome
- State of the Art Fitness Center
- Elegant Pool & Spa
- In Home Washer & Dryer
- Spinning & Yoga Room
- Resident Lounge, Juice Bar & Game Room
- Extra Storage Available
- Kids Corner & Business Center
- Close to Parks, Trails, Shopping & Dining
- Easy Access to I-94/694, & Hwy 169
Eagle Ridge7020 Magda Drive Maple Grove, MN 5536945.0813435 -93.414627- Near 55304+−Preview DetailsCollapse Details
Eagle Ridge has 1 and 2 bedroom apartments in Maple Grove with A/C, dishwashers, w/d hookups, huge patio or balcony, & large closets. Underground heated garage parking with car wash bay, basketball & tennis courts, outdoor pool & fitness center. Storage locker, heat and parking included. Eagle Ridge is near I-694, Hwy 169, near Arbor Lake Shoppes, Albertville Mall, Main Street in Maple Grove, Elm Creek Park & transit center.
- Spacious 1 and 2 Bedrooms
- Outdoor Pool with Sun Deck & Sauna
- Heat Included in Rent
- Underground Heated Garage with Car Wash Bay
- Fitness Center
- Boat Access To Eagle Lake
- Beautiful Grounds with Picnic Areas
- Volleyball and Basketball Courts
- Large Walk-Out Patios or Balconies
- Resident Services Including Package Delivery
- Near Shoppes at Arbor Lakes in Maple Grove
- Highway Access to I-694, I-169, and I-494
The Birchwood Apartments16600 92nd Ave. N Maple Grove, MN 5531145.122104 -93.491381- Near 55304+−Preview DetailsCollapse Details
Spacious 1 and 2 bedroom pet friendly apartments for rent in Maple Grove, MN. The Birchwood Apartments feature air conditioning, dishwasher, full washer and dryer, and patio/balcony are in every apartment. Heat is included in your rent. Underground heated garage parking, fitness center and controlled access entry are some of the amenities included in The Birchwood Apartments. This convenient location is near Highways 94 and 101 in Maple Grove.
- 2 Bedroom / 1 Bath Available Now!
- Pet Friendly - Cats and Dogs Welcome
- Underground Heated Garage Parking
- Fitness Center, In-Home Washer and Dryer
- Heat Included in Rent
- Log Balconies or Patios in Every Apartment
- Air Conditioning, Dishwasher, and Garbage Disposal
- Apartments are Near Boston Scientific
- Entertainment Room w/Kitchen and Screened Porch
- Computer Room with Internet Access
- Close to Restaurants, Theaters & Shopping
- Near Parks, Lakes and Preserve
Springbrook111 83rd Ave. NE Fridley, MN 5543245.118713355108 -93.267291795845- Near 55304+−Preview DetailsCollapse Details
Springbrook Apartments offers 1, 2 and 3 bedroom apartments for rent in Fridley, MN next door to the SpringBrook Nature Center and near dining, shopping & entertainment. The community has indoor/outdoor pools, volleyball, tennis, jogging path, basketball, putting green, fitness center & social events. Springbrook Apartments are pet friendly & feature air conditioning, ceiling fans, dishwasher, walk-in closets, onsite laundry, patio or balcony and garage parking.
- 1, 2 and 3 Bedrooms. Some Smoke Free Buildings
- Cats and Small Dogs Welcome
- Business Center and Rec Room
- Party, Social Rooms
- Indoor/Outdoor Pool
- Playground
- Next to Northtown Mall
- Tennis, Basketball and Volleyball Courts
- Jogging and Walking Paths
- Microwave and Dishwasher
- Beautiful Grounds With Ponds and Fountains
- Spacious Floorplans
Cutters Grove2901 Cutters Grove Ave Anoka, MN 5530345.210767229647 -93.413642259191- Near 55304+−Preview DetailsCollapse Details
Cutters Grove Estates offers 1, 2 and 3 bedroom apartments with private garages for rent in Anoka, MN near the Missisippi River. The community of Cutter's Grove has fulfilling activities such as fishing dock, picnic gazebos, playground, ball fields, hiking trails, outdoor pool, tennis, basketball and a community room. Apartments feature a patio or balcony, walk-in closets, breakfast bars & washers and dryers in select. Garage parking is available and cats and dogs are welcome.
- 24/7 Leasing Info By Phone
- Heat Paid, Water, Garbage Included
- Cats & Dogs Welcome
- Washer & Dryer in Select Units
- Private Patio or Balcony
- Oversized Sparkling Outdoor Pool
- Underground Heated Parking or Detached Garage
- Remodeled Party/Community Room
- Generous Walk-In Closet Space
- Resident Dog Park
- Picnic Gazebos, BBQ Area
- Private Entries
The Willows6232 65th Avenue North Brooklyn Park, MN 5542945.07293663919 -93.359643146396- Near 55304+−Preview DetailsCollapse Details
The Willows Apartments offer 1, 2, & 3 bedroom apartments in Brooklyn Park, MN. Cat friendly, air conditioning, modern kitchens with a dishwasher, and a patio or balcony. Community features include an indoor pool, outdoor pool, sauna, playground, fitness center with free weights and picnic areas. Public transportation, parks & lakes nearby. Close to 694/94 and County Rd. 81 Heat included in Rent.
- 1, 2 and 3 Bedroom Apartments
- Fitness Center
- Garages Available
- Sand Volleyball
- 24 Hour Maintenance
- Indoor and Outdoor Pools, Saunas
- Spacious Walkout Patios or Balconies
- Oak or Euro Style Kitchen Cabinets
- Laundry Center on Each Floor
- Picnic Area and Playground
- Cable TV Available
- Oversize Closets
Equinox2808 Silver Lane NE Minneapolis, MN 5542145.041778104297 -93.221543188785- Near 55304+−Preview DetailsCollapse Details
Equinox apartments offer large 1, 1 plus den and 2 bedroom apartments for rent in Minneapolis, MN. Beautifully remodeled & conveniently located minutes to downtown Minneapolis. Rent includes heat, garage parking and extra storage. The community of Equinox has a clubhouse, indoor & outdoor pools, fitness center, playground and laundry rooms. Apartments feature walk-in closets, patio or balcony, dishwasher, disposal, air conditioning and cat friendly apartments are available.
- Paid Heat, Water, Sewage, & Trash Disposal
- Garage Parking and Extra Storage Space Included
- Completely Remodeled Property w/Beautiful Grounds
- Spacious 1, 1 + Den, 2, & 2 Bedroom + Den Homes
- Kitchen with Dishwasher and Garbage Disposal
- On the Busline; Near Downtown Minneapolis
- Air Conditioning and Private Patios or Balconies
- Indoor & Outdoor Pools, Clubhouse w/Fitness Center
- On the Busline; Near Downtown Minneapolis
- Adjacent to Shopping, Restaurants, Walking Trails
- BBQ Grilling and Picnic Area
- 24-Hour Laundry Facilities
Huntington Place5805 73rd Avenue North Brooklyn Park, MN 5542945.0872621 -93.3520466- Near 55304+−Preview DetailsCollapse Details
Huntington Place Apartments offer 1 bedroom apartments for rent in Brooklyn Park, MN. Huntington Place Apartments are cozy w/huge lay-outs, spacious closets, large kitchens, and dishwasher. Pet friendly apartments, cats and dogs welcome! Within the community is a clubhouse/party room w/free WiFi, fitness center & dance studio, indoor/outdoor pools, year-round hot tub and private garage parking is available.
- Spacious One Bedroom Apartments For Rent
- Pet Friendly Apartments, Cats & Dogs Welcome
- 2 Story Clubhouse/Party Room, Free WiFi
- Largest Privately Owned Outdoor Pool
- Exercise Room & Aerobic Studio
- Year-Round Outdoor Hot Tub & Indoor Pool
- Dishwasher, Walk-Through Kitchen
- Dog Park Now Open!
- Abundant Closets and Storage
- Gazebos/Picnic Area
- Ample Off Street Parking
- Breathe Easier! Ask about Smoke Free Living!
Carriage Oaks12373 Oak Park Blvd. Blaine, MN 5543445.194798247368 -93.245380548988- Near 55304+−Preview DetailsCollapse Details
Carriage Oaks is offers spacious 1 and 2 bedroom apartments for rent in Blaine, MN just north of Minneapolis and St. Paul. These pet friendly apartments have walk-in closets, ceiling fans, in-home washer in dryer (in select homes), and heat, water, and trash are all included in rent! Community amenities at Carriage Oaks include an outdoor pool, an indoor pool, jacuzzi, sauna, tennis court, and volleyball court. Carraige Oaks is conveniently located in Blaine, near Hwy 65 and 14.
- Heat, Water & Trash Included in Rent
- Washer and Dryer in Some Apartments
- Brand New Kitchen Appliance Package w/microwave
- Pet Friendly - Cats and Dogs Welcome!
- Heated Indoor and Outdoor Pools
- Jacuzzi & Sauna, Clubhouse
- Fitness Center with Free Weights
- Tennis Court & Sand Volleyball Court
- Immaculate Interiors and Beautiful Grounds
- Near Bunker Hills Recreation & Golf Course
- Accept MAJOR CREDIT CARDS
- Off of Hwy 65 and County Road 14
Carrington Drive6910 Humboldt Ave N Apt 204 Brooklyn Center, MN 5543045.080454050473 -93.300165496321- Near 55304+−Preview DetailsCollapse Details
Carrington Drive offers studio, 1 and 2 bedroom apartments for rent in Brooklyn Center, MN, conveniently located just north of Minneapolis near public transportation, I-94, Hwy 100 and I-694 for easy metro access. The community of Carrington Drive has garage parking, extra storage, controlled entry, outdoor pool, BBQ grill, picnic area and beautiful courtyard. Apartments feature individually controlled heat & air conditioning, ceiling fans, window coverings and heat,water, sewer, trash are paid.
- Studio, One & Two Bedroom Apartment
- Heat, Water, Sewer & Trash Removal Included
- Individually Controlled Heat & Air Conditioning
- Outdoor Swimming Pool
- Ceiling Fans in Every Bedroom
- On Bus Line
- Cable TV Ready; Internet Ready
- close to shopping; minutes from Target & Walmart
- Near Major Interstate I-94/694; Right off Hwy 100
- Professional Onsite Management
- 24 Hour Emergency Service
- Spacious Courtyard; beautifully landscaped
Colonial Estates11360 NW Robinson Dr Coon Rapids, MN 5543345.177715 -93.311029- Near 55304+−Preview DetailsCollapse Details
Colonial Estates in Coon Rapids, MN offers spacious 1, 2, and 3 bedroom apartments for rent. Apartments feature dishwasher, patio/balcony, carpet, mini blinds, and an in-home washer and dryer in some apartments. Colonial Estates is pet friendly and welcomes your dog and cat. Community features include outdoor and indoor pools, fitness center, and spa. This great Coon Rapids apartment is near several parks, off of County Road 47, near the Coon Rapids Soccer Complex.
- Spacious 1, 2 and 3 Bedroom Apartments
- Large Dogs and Cats Welcome!
- Washers and Dryers in Many Homes
- Walk-in Closets
- Brand New Kitchen Appliance Package w/microwave
- Laundry Facilities in Each Building
- Whirlpool, Sauna, Picnic Areas, and Clubhouse
- Fitness Center, Spa, Heated Indoor & Outdoor Pools
- Detached Garages Available
- Controlled Access
- Plenty of Guest Parking
- Elevators in Every Building
Garden View1417-10th St NW New Brighton, MN 5511245.064256256856 -93.205792772559- Near 55304+−Preview DetailsCollapse Details
Garden View apartments offer spacious 1 and 2 bedroom apartments for rent in New Brighton, MN. Each home is equipped with a dishwasher in the kitchen and an attached private patio or balcony. Single stall garages are available for rent. Garden View has recreational activities like the indoor and outdoor pools, billiards room, fitness center, picnic area, and a party room that is great for entertaining. Pet friendly apartments, call for more details.
- Indoor and Outdoor Pools
- Private Patio or Balcony
- Fitness Area
- Dishwasher
- Single Garage Stalls Available
- Clubhouse Party Room
- Billiards Room
- Spacious 1 & 2 Bedrooms
- Separate Dining Area
- Playground
- Picnic Areas
- Easy Access to 694 and 35W
Evans Meadows341 Evans Ave NW Elk River, MN 5533045.304753929377 -93.559312149882- Near 55304+−Preview DetailsCollapse Details
Brand New spacious and pristine 1,2 and 3 bedroom home rentals. Granite, stainless steel, walk in closets and more!
- Washer and Dryer in Each Home
- Garages
- Tenant Portal & Online Payments
- Walk-in Closets
- Outdoor Pool
- Balcony/Patio
- Courtyard
- Ceiling Fans
- Window Coverings
- Fitness Center
- Picnic Area w/Gas Grills
- Tanning
Terrace Hill14320 Dysprosium ST NW Ramsey, MN 5530345.230605 -93.401977- Near 55304+−Preview DetailsCollapse Details
Terrace Hill Apartments features unique one, two and three bedroom apartments for rent in Ramsey, MN. Homes feature walk-in closets, air conditioning, kitchen with breakfast bar, dishwasher and full size washer and dryer. The community of Terrace Hill features a guest suite, community room, picnic/bbq area, tuck under garages, fitness center, and elevator.
- Brand New & Unique 1, 2, and 3 Bedroom Apartments
- Walk-In Closets
- Full Size Washer and Dryer
- Tuck Under Garages
- Air Conditioning in All Homes
- Fitness Center
- Dishwashers
- Breakfast Bars in Select Units
- Guest Suite
- Fireplaces in Select Units
- Community Room
- Outdoor picnic area/bbq
Preserve at Commerce13600 Commerce Boulevard Rogers, MN 5537445.201666002578 -93.544358419527- Near 55304+−Preview DetailsCollapse Details
Preserve at Commerce features studio, 1, 2, and 3 bedroom apartments for rent in Rogers, MN near Albertville Outlets & Arbor Lakes Shoppes. These pet friendly apartments offer air conditioning, full size washer and dryer in home, ceiling fans, spacious walk-in closets, balcony or patio, and heat is included in rent! Preserve at Commerce community amenities include 24 hr tanning area and fitness center, underground parking, party room with kitchen, billiards room, and indoor pool and whirlpool.
- Underground Heated Garage Parking Included in Rent
- In-Home Full Size Washer and Dryer
- 24 Hour Fitness Center
- Pet Friendly Apartments - Dogs and Cats Welcome
- Indoor Pool and Whirlpool
- Heat is Included in Rent
- 24 Hour Tanning Room
- Large Walk-in Closets
- Community Room and Billiards Room
- Extra Storage Units Available
- Car Wash
- Near Albertville Outlets & Shoppes at Arbor Lakes
Brookside Manor1301 67th Ave. N Brooklyn Center, MN 5543045.07722 -93.297839- Near 55304+−Preview DetailsCollapse Details
Brookside Manor feature affordable 2 bedroom apartments for rent in Brooklyn Center, MN. Enjoy the community amenties inlcuding a heated outdoor pool, courtyard with a play area, and controlled access entry. Each apartment at Brookside offers air conditioning, dishwasher, and garbage disposal. There are garages available as well. Heat is included in your rent! Brookside Manor is pet friendly and welcomes your dog and cat. Located near 694 in with convenient access to highways 100 and 94.
- Heat Included in Rent
- School bus stop right outside front door!
- Spacious and Affordable 2 Bedroom Homes
- Garages Available
- Dishwaser, Disposal, and Air Conditioning
- Heated Outdoor Pool
- Easy access to Highways 94, 694 and 100
- Nearby MTC Bus Line to Downtown Minneapolis
- Controlled Access Entry
- On-Site Staff
- Just Blocks to Schools and Several Parks
- Shady Courtyard Play Area
Just Off Main12911 Arbor Lake Pkwy. N. Maple Grove, MN 5536945.095897 -93.441331- Near 55304+−Preview DetailsCollapse Details
Just Off Main has 1, 2, and 3 bedroom townhomes for rent in Maple Grove, MN near Arbor Lakes. Townhouses have attached garages, in-home washer and dryers, air conditioning, dishwasher, fireplace, and fast internet. Just Off Main also has designer carpet, custom kitchen countertops, and glass shower enclosures. Just Off Main is near Main Street and Elm Creek Blvd. Highways 694 and 94 are also nearby as well are several parks.
- Spacious 1, 2, & 3 Bedroom Townhomes
- Private Attached Garage with Automatic Opener
- In-Home Washer and Dryer
- Private Entrance and Patio
- Bonus Computer Loft Area with Skylight
- Some Vaulted Ceilings & Gas Fireplaces
- Custom Kitchen Countertops
- Glass Shower Enclosures & Soaking Tubs
- Wood Blinds
- Central Air
- Convenient Access to Highways 94, 494, & 694
- Located near Shoppes at Arbor Lakes
Mallard Ridge13301 Maple Knoll Way Maple Grove, MN 5536945.107501599586 -93.448974827768- Near 55304+−Preview DetailsCollapse Details
Mallard Ridge offers 1, 2, and 3 bedroom townhome style apartments for rent in Maple Grove, MN that welcome dogs and cats. Located near Hwy 94 these apartments feature in home washer and dryer, private balcony/patio, and spacious walk-in closets. The community amenities of Mallard Ridge offer 3 outdoor pools, hot tub, remodeled fitness center, DVD library, tanning center, playground, remodeled party room, and tuck under garage parking all near jogging trails in Maple Grove.
- 1, 2 and 3 Bedroom Townhome Style Apartments
- Washer and Dryer In-Home in Separate Room
- 3 Sparkling Outdoor Pools
- Private Patio or Balcony
- Cats and Dogs Welcome in Select Buidlings.
- Energy Efficient Air Conditioning and Furnace
- Townhome-Style Entry, Semi Private Garage
- Newly Remodeled Fitness Center
- Dishwasher and New Maple Cabinets
- Playground
- Spacious Walk-In Closets
- Clubhouse with Fireplace and Kitchen
To see more Apartments, try broadening your search.
1049 Minneapolis Metro apartments
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- Storage
Friday, 28 Feb 2014
HGST Debuts Touro S Mobile External Hard Drives at the IT Show
Debuting at the IT Show 2014, HGST's Touro S mobile external hard drives each come encased in a slim, aluminum enclosure, and deliver two levels of data protection - local backup and cloud storage, to help safeguard, transport, store and access your files from anywhere.
Tuesday, 25 Feb 2014
SanDisk Announces Its First Dual USB Drive and Premium USB 3.0 Flash Drive
SanDisk has announced its Ultra Dual USB Drive that houses both a micro-USB and a USB 2.0 connector, and its Extreme Pro USB 3.0 flash drive which offers professional-grade performance with speeds up to 60 times faster than standard USB 2.0 drives.
Seagate Targets Video Analytics Applications with 7th-gen Surveillance HDD
Designed to scale video storage, the drive now incorporates rotational vibration (RV) sensors enabling it to reliably perform in systems with up to 16 drives, making it ideal for SMEs where bulk storage for HD video and video analytics are highly valued.
Western Digital Introduces the Purple, a HDD Designed for Video Surveillance
Recognizing the growing need for storage for video surveillance systems, Western Digital has just announced the Purple, a HDD specifically designed for video surveillance purposes.
Tuesday, 18 Feb 2014
Super Talent Announces New RAIDDrive II Plus PCIe SSDs
Super Talent has just announced its next generation of PCIe SSDs - the RAIDDrive II Plus - featuring read and write speeds of up to 3000 MB/s.
Toshiba Announces The World's Largest Capacity Non-Helium HDDs
Toshiba has just announced the industry's largest non-helium gas filled hard disk drives with 5TB of capacity.
Thursday, 13 Feb 2014
WD's My Cloud Lets You Have a Cloud of Your Own
By connecting a My Cloud personal cloud drive to the Internet, consumers get the anywhere, any-device access they can get from the public cloud, without having to pay monthly fees or giving up control of their personal data.
Wednesday, 12 Feb 2014
Silicon Power and Kingston Announce Their SDHC/SDXC UHS-I Cards
In capacities of 16GB, 32GB, and 64GB, the cards have the ability to extend the length of 1080p Full HD videos, while supporting 4K2K recording.
Wednesday, 5 Feb 2014
Toshiba Launch FlashAir Wireless SD Card, Exceria SD Cards & Q-series SSD Online
Toshiba has announced the addition of storage products to its Singapore online store. In addition to Toshiba laptops, customers can now purchase Toshiba FlashAir Wireless SD cards, high performance Exceria SD cards, as well as Q-series SSDs online.
Toshiba Canvio Home Backup & Share Lets You Access Content Anytime, Anywhere
Available in capacities up to 3TB, the Canvio Home Backup & Share is an NAS hub for the connected home that brings together content and allows easy access to digital assets.


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Author and screenwriter says sorry for suggesting The Hurt Locker director only won Oscars because she was 'a hot woman'
Ellis delivers latest Twitter gaffe by suggesting a man wouldn't have won best director Oscar with The Hurt Locker
Ben Child: In contrast to previous publicity for the crowdfunded LA-set noir, written by Bret Easton Ellis, this looks like a Russ Meyer movie
British screenwriter Kelly Marcel is tasked with bringing the knee-trembler to the screen, much to Bret Easton Ellis's chagrin
What's made Bret Easton Ellis such a sourpuss?Barbara Ellen
Barbara Ellen: Easton Ellis has become someone someone who can be relied upon to kick off at random targets for no reason
Patrick Strudwick: Plenty of gay actors have convinced the world otherwise, so why not let one play Fifty Shades of Grey's Christian Grey?
Studio planning adaptation of EL James's erotic bestseller omits cult novelist from shortlist of four screenwriters
Ben Child: The Canyons, billed as 'contemporary LA noir', pairs Lohan with former porn star James Deen and sees the maverick storyteller writing directly for the screen for the first time
The production team behind The Social Network are to work on the film version of EL James's erotic bestseller Fifty Shades of Grey
Bret Easton Ellis has said he wants to adapt EL James's S&M e-publishing sensation 50 Shades of Grey for cinema. John Crace imagines the screenplay
Easton Ellis is teasing Twitter with ideas on casting, directors and screenwriters (himself) for the adaptation of EL James's erotic novels. Is he serious, asks Ben Child
Tim Minchin and Stephen Fry are among the new Cultural Olympiad acts, while protestors take to the stage at Stratford-upon-Avon and encourage audiences to rip up their seats
The award-winning author of HhhH tells Killian Fox about his literary Nazi hunt, having his debut novel hailed as a masterpiece by Bret Easton Ellis, and why he wants change at the Elysée Palace
US author delights Twitter followers with multi-tweet ideas session, finishing with tentative plans to start writing
Adored by young women for his clean-cut image, this male porn star has been named as the likely lead actor in Bret Easton Ellis's forthcoming film. But can an adult entertainer ever make a successful transition to Hollywood?
Jonathan Lethem's essays reveal surprising influences on his fiction. By Stuart Kelly
Kind bankers, nasty bankers, bohemian bankers and a banker called Banks
Dan Holloway: It may be set in permanently sunny Los Angeles, but things don't get much icier than inside its characters' hearts
Will Self's trademark extravagant prose abounds in his surreal 'travelogue', writes Anthony Cummins
Chris Michael: Reading this dark tale of suburban psychosis under the Aegean sun was like smuggling trouble into paradise
Taxi Driver writer and American Psycho novelist are to join forces on a new shark horror film
Ben Myers: Good novels have a pretty inglorious history of providing 'the book' for musical adaptations, but I'm kind of intrigued to know what Broadway will make of Patrick Bateman
The author of American Psycho talks to John Mullan about materialism, violence, and how the novel sprang from the rage he felt when he moved to New York
The G2 interview Bret Easton Ellis: 'So you're a misogynist, a racist – so what? Does it make your art less interesting?'
The American author on how pain is the inspiration for all his books, getting off drugs and telling the truth
American Psycho author Bret Easton Ellis argues love and sadism are linked, and that technology has stopped us feeling
Bret Easton Ellis tells Elizabeth Day why his abusive father spurred him to become a writer
Week four: readers' responses