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Publications (39)136.41 Total impact
- SourceAvailable from: Yao Zhenyu[Show abstract] [Hide abstract]
ABSTRACT: Macrophages are responsible for defending against diverse pathogens and play a crucial role in the innate immune system. Macrophage's lifespan is determined by homeostatic balance between survival and apoptosis. Here we report that tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) triggers both apoptosis and autophagy in human U937 cells. Inhibition of autophagy facilitates TRAIL-induced apoptosis, suggesting that autophagy of macrophages protects against TRAIL-induced apoptosis. TRAIL treatment influences the expression of death receptors, indicating that TRAIL-induced apoptosis and autophagy are mediated by death receptors. RIP1 ubiquitination and expression regulate apoptosis and autophagy. Furthermore, expression and bioactivity of the p43/41-caspase-8 variant are critical to TRAIL-induced autophagy and apoptosis. Knockdown of RIP1 suppresses autophagy in macrophage. These data demonstrate that RIP1 is essential for the regulation of death receptor mediated autophagy and apoptosis. The results in this study contribute to understanding the regulation of autophagy and apoptosis in macrophages, and shed lights on death receptor-targeted therapy for cancer, inflammation and autoimmune diseases. Copyright © 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.Molecular Oncology 12/2014; 9(4). DOI:10.1016/j.molonc.2014.12.004 · 5.94 Impact Factor
Dataset: A Novel Anti-human DR5 Monoclonal Antibody with Tumoricidal Activity Induces Caspase-dependent and Caspase-independent Cell Death * Yabin Guo[Show abstract] [Hide abstract]
ABSTRACT: Like anti-Fas monoclonal antibodies, some monoclonal antibod-ies against tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptors have tumoricidal activity too. In this article we report a novel mouse anti-human DR5 monoclonal antibody, AD5– 10, that induces apoptosis of various tumor cell lines in the absence of second cross-linking in vitro and showed strong tumoricidal activity in vivo. AD5–10 does not compete with TRAIL for binding to DR5 and synergizes with TRAIL to induce apoptosis of tumor cells. AD5–10 induces both caspase-dependent and caspase-inde-pendent cell death in Jurkat cells, whereas TRAIL induces only caspase-dependent cell death. We show for the first time that DR5 can mediate caspase-independent cell death, and DR5 can mediate distinct cell signals when interacting with different extracellular proteins. Studies on AD5–10 help us to understand more on the functions of DR5 and may provide new ideas for cancer immunotherapy. Tumor necrosis factor (TNF) 2 -related apoptosis-inducing ligand (TRAIL) is a member of the TNF superfamily with the ability to induce apoptosis in a wide variety of transformed cell lines of diverse origin (1). At least five receptors for TRAIL have been identified so far. Two of them, DR4 (TRAIL-R1) and DR5 (TRAIL-R2/TRICK2) (2– 4), are capa-ble of transducing an apoptosis signal, whereas the other three, decoy receptor (DcR) 1 (TRAIL-R3) (5), DcR2 (TRAIL-R4) (6), and osteopro-tegerin (7), serve as decoy receptors to block TRAIL-mediated apopto-sis. DR4 and DR5 share a common intracellular domain, called the death domain (DD), which is indispensable for initiation of the intracellular signaling cascade leading to cell death. The death domain motif is also found in the cytoplasmic adaptor proteins, such as Fas-associated pro-tein with death domain (FADD) (8), TNF-R1-associated death domain protein (TRADD) (9), and receptor-interacting protein (RIP) (10), etc. These adaptor proteins are essential for the intracellular signals medi-ated by DR4 and DR5 (11). TRAIL triggers multiple cell signals, includ-ing the activation of apoptotic caspase cascade, c-Jun N-terminal kinase (JNK), p38 mitogen-activated protein kinase (MAPK) and NF-B (11–13). In contrast to TNFs and Fas ligand, TRAIL has been known to induce apoptosis in a variety of tumor cells and some virally infected cells but not in most normal cells. The potential and safety of soluble TRAIL (sTRAIL) as an anticancer therapeutic agent have been demonstrated in mice and non-human primates (14, 15). However, increasing experi-mental evidence on TRAIL-inducing apoptosis of normal cells (espe-cially hepatocytes) were reported in recent studies (16, 17), arguing against the potential usefulness and safety of soluble TRAIL in cancer therapy. Meanwhile, there were also reports demonstrating that recom-binant TRAIL without exogenous sequence tags was nontoxic to human hepatocytes both in vitro (18, 19) and in chimeric mice (20). In addition to sTRAIL, monoclonal antibodies (mAbs) against TRAIL receptors with tumoricidal activity are also potential candidates for cancer ther-apy. There are a number of agonistic mAbs against human DR4 or DR5 reported in previous studies (21–23), most of which need cross-linkers to ensure effective killing of tumor cells (21, 22). In 2001, Ichikawa et al. (23) reported a mouse anti-DR5 mAb, TRA-8, that showed strong tumoricidal activity in the absence of cross-linking and had no hepato-cyte toxicity. TRA-8 competes with TRAIL for binding to DR5 and almost entirely mimics the apoptosis-inducing mechanism of TRAIL. The authors believed that DR5 was not sufficient to trigger apoptosis in normal hepatocytes (23). However, a recent study showed that at least some anti-DR5 and anti-DR4 mAbs did induce human hepatocytes apo-ptosis (24). Therefore, we cannot draw a definite conclusion on the hepatocyte toxicity of soluble TRAIL or mAbs against TRAIL receptors now. Studies on the mechanism of anti-DR5 mAbs will help us to under-stand the complicated signal pathways mediated by DR5. In this article, we report studies on AD5–10, a novel monoclonal antibody against human DR5. AD5–10 induces apoptosis in various tumor cell lines in the absence of second cross-linking in vitro and exhibits a strong tumoricidal activity in vivo. AD5–10 does not induce cell death of human normal hepatocytes and primary peripheral blood lymphocytes, and the injection with high doses of AD5–10 in mice causes no toxic reaction in liver, spleen, and kidney. Unlike TRA-8, AD5–10 does not compete with TRAIL for binding to DR5, and there is a synergistic effect between TRAIL and AD5–10 on their tumoricidal activity. Downstream cell signals induced by AD5–10 and TRAIL were also compared. Both TRAIL and AD5–10 activate caspase cascade and induce a classical apoptosis in Jurkat cells. Interestingly, AD5–10 kills
Article: Human T-Cell Leukemia Virus Type 1 Tax-Deregulated Autophagy Pathway and c-FLIP Expression Contribute to Resistance against Death Receptor-Mediated Apoptosis[Show abstract] [Hide abstract]
ABSTRACT: The human T-cell leukemia virus type 1 Tax protein is considered to play a central role in the process that leads to adult T-cell leukemia/lymphoma (ATL) and HTLV-1-associated myelopathy/tropical spastic paraparesis (HAM/TSP). HTLV-1 Tax-expressing cells show resistance to apoptosis induced by Fas ligand (FasL) and tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL). The regulation of Tax on autophagy pathway is recently reported in HeLa cells and peripheral T cells, but the function and underlying molecular mechanism of the Tax-regulated autophagy are not yet well defined. Here, we report that HTLV-1 Tax deregulates autophagy pathway, which plays a protective role during the death receptor (DR)-mediated apoptosis of human U251 astroglioma cells. The cellular FLICE-inhibitory protein (c-FLIP), which is upregulated by Tax, also contributes to the resistance against DR-mediated apoptosis. Both Tax-induced autophagy and Tax-induced c-FLIP expression require Tax-induced activation of IκB kinases (IKK). Furthermore, Tax-induced c-FLIP expression is regulated through the Tax-IKK-NF-κB signaling pathway, whereas Tax-triggered autophagy depends on the activation of IKK, but not the activation of NF-κB. In addition, DR-mediated apoptosis is correlated with the degradation of Tax, which can be facilitated by the inhibitors of autophagy.Journal of Virology 12/2013; 88(5). DOI:10.1128/JVI.03025-13 · 4.65 Impact Factor
Article: TRAIL receptor deficiency sensitizes mice to dextran sodium sulfate induced colitis and colitis-associated carcinogenesis.[Show abstract] [Hide abstract]
ABSTRACT: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) and its receptor (TRAIL-R) play important roles in immune regulation and cancer cell death. Although TRAIL has been shown to induce chemokine release in various tumor cells, the function of TRAIL-R in the development of colitis and colitis-associated carcinogenesis has not been explored.In this study, we found that TRAIL-R-deficient mice exhibited a higher incidence of colitis and colitis-associated cancer than that of wild-type (WT) mice, and TRAIL-R expression was down-regulated in WT mice that were fed dextran sulfate sodium. Chemokines, including CCL2 and CXCL1, were highly expressed in the serum and inflammatory colon tissues of TRAIL-R(-/-) mice compared to WT mice, and TRAIL-R(-/-) mice showed a marked infiltration of immune cells during colitis. Hyperactivation of JNK and NF-κB in colon epithelial cells was also observed, which correlated with the severity of colonic inflammation in TRAIL-R(-/-) mice.These data suggest that TRAIL-R plays a protective role in chemical-induced colon injury and negatively regulates mucosal immune responses. This article is protected by copyright. All rights reserved.Immunology 10/2013; 141(2). DOI:10.1111/imm.12181 · 3.74 Impact Factor
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ABSTRACT: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) is considered a promising agent for cancer therapy, as this molecule induces apoptosis specifically in various cancer cells. Apart from apoptosis, TRAIL also induces non-apoptotic signals, such as those for autophagy, proliferation, and metastasis in cancer cells. In the present study, we report that TRAIL suppressed CXCR4-mediated human breast cancer MDA-MB-231 cell migration by up-regulating miR-146a expression through NF-κB. TRAIL receptor 1 (TRAIL-R1, DR4) was highly expressed in TRAIL-treated MDA-MB-231 cells. A neutralization antibody against DR4 specifically blocked TRAIL-induced NF-κB activation and miR-146a expression. These results were confirmed in a human breast cancer xenograft mouse model, suggesting that TRAIL significantly enhanced miR-146a expression and suppressed CXCR4 expression, indicating that TRAIL-induced miR-146a up-regulation is negatively associated with CXCR4 expression. These findings suggest that TRAIL-induced miR146a expression suppresses CXCR4-mediated human breast cancer migration and provide further insight into the non-apoptotic function of TRAIL in the prevention of metastasis as a therapy for breast cancer. This article is protected by copyright. All rights reserved.FEBS Journal 05/2013; DOI:10.1111/febs.12323 · 3.99 Impact Factor
Article: TRAIL suppresses tumor growth in mice by inducing tumor-infiltrating CD4(+)CD25 (+) Treg apoptosis.[Show abstract] [Hide abstract]
ABSTRACT: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), a promising and novel anticancer cytokine, specifically kills numerous tumor cells by apoptosis. However, some malignancies are resistant to TRAIL treatment in clinical trials, thus limiting its therapeutic potential. In the present study, the TRAIL-resistant murine hepatocellular carcinoma cell line Hepa1-6 was used to elucidate the physiological significance of TRAIL resistance, especially with respect to the immune regulatory function of TRAIL. Hepa1-6 cells were resistant to TRAIL-induced apoptosis in vitro; however, intratumoral injection of recombinant soluble TRAIL inhibited tumor growth and prolonged survival time in tumor-bearing mice. Local TRAIL treatment decreased the number of intratumoral CD4(+)CD25(+)Foxp3(+) regulatory T cells (Tregs) but did not affect CD4(+)CD25(+)Foxp3(+) Tregs in the draining lymph nodes and spleen. Further investigation showed that TRAIL induced apoptosis of tumor-activated CD4(+)CD25(+)Foxp3(+) Tregs, but not of CD4(+)CD25(-) T cells. Moreover, mouse TRAIL receptor DR5 expression was detected on the surface of the tumor-infiltrating CD4(+)CD25(+)Foxp3(+) Tregs, but not on naïve CD4(+)CD25(+)Foxp3(+) Tregs. Interestingly, intratumoral injection of TRAIL not only decreased the number of CD4(+)CD25(+)Foxp3(+) Tregs but also increased the number of tumor-specific CD8(+) CTL and augmented their cytotoxicity to the tumor cells. These data provide the novel evidence for an immune regulatory function of TRAIL and may shed light on the clinical application of TRAIL.Cancer Immunology and Immunotherapy 11/2012; DOI:10.1007/s00262-012-1370-x · 3.94 Impact Factor
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ABSTRACT: Construct expression vectors of pCMV-DR4-HA and pCMV-PRMT5-Flag, and transfect them into HEK293 cells to identify the interaction between TRAIL-R1 and PRMT5 and the molecular mechanism underlying DR4-mediated inhibition of chemokine CCL20 release via TRAIL receptor 1 (DR4). Inflammatory cytokine was detected by RT-PCR and ELISA after TRAIL-R1 and/or PRMT5 transfection, respectively. NF-κB activity was detected by Dual Luciferase Reporter Gene Assay. ERK1/2 phosphorylation was analyzed by Western blot. PRMT5 could inhibit DR4-activated NF-κB activity and ERK1/2 phosphorylation. PRMT5 could inhibit NF-κB activition, ERK1/2 phosphorylation as well as CCL20 secretion via binding with DR4 in HEK293 cell, suggesting that PRMT5 may involve in DR4 dependent immune regulation.Chinese Science Bulletin 11/2012; 57(33). DOI:10.1007/s11434-012-5511-6 · 1.37 Impact Factor
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ABSTRACT: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) induces apoptosis in a variety of tumor cells. TRAIL receptor 2 (DR5) expression is high in tumor cells, transformed cells, and clinical tumor specimens and is low in most normal cells and tissues; therefore, DR5 is considered an attractive target for cancer therapy. In this study, HMCAZ5, a novel mouse-human chimeric antibody based on AD5-10, was generated and stably expressed in CHO-dhfr(-) cells. Highly purified HMCAZ5 exhibits a high affinity for the receptor that is equal to the parental mouse antibody, induces apoptosis in various cancer cells but not in normal hepatocytes, and elicits both antibody-dependent cellular cytotoxicity and complement-dependent cytotoxicity in various human cancer cells. The anthracycline anticancer drug epirubicin (EPB) synergizes the cytotoxicity of HMCAZ5 in cancer cells by upregulating DR5 expression on the cell surfaces, enhancing p53 expression, Bid cleavage, and JNK phosphorylation and downregulating c-FLIP expression and Akt phosphorylation. Moreover, HMCAZ5 alone suppresses tumor growth, and EPB augments the tumoricidal activity in human colorectal and hepatocellular tumor xenografts in athymic nude mice. These data suggest that the anti-DR5 chimeric antibody HMCAZ5 may have a clinical use and represents a useful immunological strategy, in combination with chemotherapy, for the treatment of cancer.International Union of Biochemistry and Molecular Biology Life 09/2012; 64(9):757-65. DOI:10.1002/iub.1064 · 2.76 Impact Factor
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ABSTRACT: Age-associated immune dysfunction, characterized by increased systemic levels of cytokines, manifests as an increased susceptibility to infections. Thus, understanding these negative regulators of the immune response has paved the way to delineating signaling pathways that impact immune senescence. In the present study, we found that miR-146a, which negatively regulated the expression of IL-1β and IL-6, was highly expressed in aged mice. However, there was a lack of response to the stimulation of lipopolysaccharide (LPS) and proinflammatory cytokines in macrophages of aged mice. As a result, the negative feedback regulation loop with miR-146a involving down-regulation of inflammation factors was interrupted in aged mice. Aberrant NF-κB binding to the miR-146a promoter was demonstrated to be associated with the abnormal expression of miR-146a in aged mice. The DNA methyltransferase inhibitor (5-aza-2-deoxycytidine) and the histone deacetylase inhibitor [trichostatin A (TSA)] both significantly up-regulated miR-146a transcriptional activation by altering the DNA-binding activity of NF-κB in macrophages isolated from aged mice, which suggests that DNA methylation and histone acetylation are involved in the suppression of age-dependent miR-146a expression. Additionally, high levels of histone deacetylase (HDACs) expressions contributed to the inhibition of miR-146a expression in LPS-stimulated macrophages from aged mice in vitro. While the suppression of HDACs activities by TSA could improve LPS-induced inflammatory responses owing to up-regulation of miR-146a expression in macrophages from aged mice. These data indicate that the dysregulated expression of miR-146a results in the age-associated dysfunction of macrophages, and miR-146a may be a good target for the treatment of age-related inflammatory diseases.Aging cell 02/2012; 11(1):29-40. DOI:10.1111/j.1474-9726.2011.00757.x · 5.94 Impact Factor
Article: 2A Peptide-based, Lentivirus-mediated Anti-death Receptor 5 Chimeric Antibody Expression Prevents Tumor Growth in Nude Mice[Show abstract] [Hide abstract]
ABSTRACT: Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL), a member of the TNF superfamily, induces tumor cell death via death receptors on target cells, without adverse effects on most normal cells. Its receptors are therefore an attractive target for antibody-mediated tumor therapy. Here, we report the creation of a lentivirus vector constructed by linking the heavy chain and the light chain of the antibody with a 2A/furin self-processing peptide in a single open reading frame that expresses a novel chimeric antibody (named as zaptuximab) with tumoricidal activity, which is consisted of the variable region of a mouse anti-human DR5 monoclonal antibody, AD5-10, and the constant region of human immunoglobulin G1. Lentivirus-expressed zaptuximab bound specifically to its antigen, DR5, and exhibited significant apoptosis-inducing activity in various tumor cell lines. The packaged recombinant virus lenti-HF2AL showed strong apoptosis-inducing activity in vitro. Meanwhile, inoculated subcutaneous human colon HCT116 tumor formation in nude mice were inhibited significantly. Moreover, there was a synergistic effect of mitomycin C (MMC) on the observed tumoricidal efficacy, prolonging the life span of nude mice with orthotopic human lung tumor cancers. These data suggest that lentivirus-mediated, 2A peptide-based anti-DR5 chimeric antibody expression may have clinical utility as an anticancer treatment and may represent a rational adjuvant therapy in combination with chemotherapy.Molecular Therapy 09/2011; 20(1):46-53. DOI:10.1038/mt.2011.197 · 6.43 Impact Factor
Article: HIV Infection Enhances TRAIL-Induced Cell Death in Macrophage by Down-Regulating Decoy Receptor Expression and Generation of Reactive Oxygen Species[Show abstract] [Hide abstract]
ABSTRACT: Tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) could induce apoptosis of HIV-1-infected monocyte-derived macrophage (MDM), but the molecular mechanisms are not well understood. By using an HIV-1 Env-pseudotyped virus (HIV-1 PV)-infected MDM cell model we demonstrate that HIV-1 PV infection down-regulates the expression of TRAIL decoy receptor 1 (DcR1) and 2 (DcR2), and cellular FLICE-inhibitory protein (c-FLIP), but dose not affect the expression of death receptor 4 and 5 (DR4, DR5), and Bcl-2 family members in MDM cells. Furthermore, recombinant soluble TRAIL and an agonistic anti-DR5 antibody, AD5-10, treatment stimulates reactive oxygen species (ROS) generation and JNK phosphorylation. HIV infection facilitates TRIAL-induced cell death in MDM by down-regulating the expression of TRAIL decoy receptors and intracellular c-FLIP. Meanwhile, the agonistic anti-DR5 antibody, AD5-10, induces apoptosis synergistically with TRAIL in HIV-1-infected cells. ROS generation and JNK phosphorylation are involved in this process. These findings potentiate clinical usage of the combination of TRAIL and AD5-10 in eradication of HIV-infected macrophage and AIDS.PLoS ONE 04/2011; 6(4):e18291. DOI:10.1371/journal.pone.0018291 · 3.53 Impact Factor
Article: Adeno-associated virus-mediated anti-DR5 chimeric antibody expression suppresses human tumor growth in nude mice[Show abstract] [Hide abstract]
ABSTRACT: In the present study we demonstrate that adeno-associated virus (AAV)-mediated anti-DR5 (death receptor 5) mouse-human chimeric antibody (shorten as Adximab) expression significantly suppressed tumor cell growth by inducing apoptosis both in vitro and in vivo. The viral-expressed and cell-secreted Adximab efficiently bound DR5 with an affinity of 0.7nM and induced apoptosis of various tumor cells, but not normal cells. A single intramuscular injection of recombinant AAV particles resulted in a stable expression of Adximab in mouse serum for at least 70days. AAV-mediated Adximab expression led to a significant suppression of tumor growth in nude mice receiving xenografts of human liver and colon cancer. These data suggest that chimeric antibody gene transfer may provide an alternative strategy for the therapy of varieties of cancers.Cancer letters 03/2011; 302(2):119-27. DOI:10.1016/j.canlet.2011.01.001 · 5.02 Impact Factor
Article: Synergistic antitumor effect of AAV-mediated TRAIL expression combined with cisplatin on head and neck squamous cell carcinoma[Show abstract] [Hide abstract]
ABSTRACT: Adeno-associated virus-2 (AAV-2)-mediated gene therapy is quite suitable for local or regional application in head and neck cancer squamous cell carcinoma (HNSCC). However, its low transduction efficiency has limited its further development as a therapeutic agent. DNA damaging agents have been shown to enhance AAV-mediated transgene expression. Cisplatin, one of the most effective chemotherapeutic agents, has been recognized to cause cancer cell death by apoptosis with a severe toxicity. This study aims to evaluate the role of cisplatin in AAV-mediated tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) expression and the effect on HNSCC both in vitro and in vivo. Five human HNSCC cell lines were treated with recombinant soluble TRAIL (rsTRAIL) and infected with AAV/TRAIL to estimate the sensitivity of the cancer cells to TRAIL-induced cytotoxicity. KB cells were infected with AAV/EGFP with or without cisplatin pretreatment to evaluate the effect of cisplatin on AAV-mediated gene expression. TRAIL expression was detected by ELISA and Western blot. Cytotoxicity was measured by MTT assay and Western blot analysis for caspase-3 and -8 activations. Following the in vitro experiments, TRAIL expression and its tumoricidal activity were analyzed in nude mice with subcutaneous xenografts of HNSCC. HNSCC cell lines showed different sensitivities to rsTRAIL, and KB cells possessed both highest transduction efficacy of AAV and sensitivity to TRAIL among five cell lines. Preincubation of KB cells with subtherapeutic dosage of cisplatin significantly augmented AAV-mediated transgene expression in a heparin sulfate proteoglycan (HSPG)-dependent manner. Furthermore, cisplatin enhanced the killing efficacy of AAV/TRAIL by 3-fold on KB cell line. The AAV mediated TRAIL expression was observed in the xenografted tumors and significantly enhanced by cisplatin. AAV/TRAIL suppressed the tumors growth and cisplatin augmented the tumoricidal activity by two-fold. Furthermore, Combination treatment reduced cisplatin-caused body weight loss in nude mice. The combination of AAV-mediated TRAIL gene expression and cisplatin had synergistic therapeutic effects on head and neck cancers and reduced the potential toxicity of cisplatin. These findings suggest that the combination of AAV/TRAIL and cisplatin may be a promising strategy for HNSCC therapy.BMC Cancer 02/2011; 11:54. DOI:10.1186/1471-2407-11-54 · 3.32 Impact Factor
Article: Tumor necrosis factor (TNF)-related apoptosis-inducing ligand (TRAIL) induces chemotactic migration of monocytes via a death receptor 4-mediated RhoGTPase pathway.[Show abstract] [Hide abstract]
ABSTRACT: This study tested the hypothesis that TRAIL could play a role in regulating monocyte migration. TRAIL has been widely studied for its anti-tumor function and signaling mechanisms. Using chemotaxis and mouse air-pouch model analyses, we determined that TRAIL-induced chemotactic migration of THP-1 human leukemia and LPS-primed primary human monocytes as well as LPS-stimulated BALB/c mouse monocytes in vivo. To expand the understanding of the TRAIL signaling pathway in this process, we found that the TRAIL receptor DR4 was highly expressed in THP-1 and LPS-primed primary monocytes but not in the non-primed primary monocytes. DR4 neutralization antibody specifically suppressed TRAIL-induced migration of the monocytes. Furthermore, PI3K, Rho GTPase and its downstream effectors, MLC and Pak1, were activated during cell migration. PI3K inhibitors and dominant negative mutants of RhoGTPase blocked monocyte migration toward TRAIL, indicating that PI3K and RhoGTPases were involved in the migration signaling. The DR4 neutralization antibody blocked the activation of PI3K and Rho GTPase effectors in the cells. Thus, these data support the hypothesis that TRAIL induces monocyte migration mediated by TRAIL receptor DR4 via the RhoGTPase signaling pathway. This study is expected to provide novel evidence of the non-apoptotic function of TRAIL in immune defense.Molecular Immunology 09/2010; 47(15):2475-84. DOI:10.1016/j.molimm.2010.06.004 · 3.00 Impact Factor
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ABSTRACT: Tumor necrosis factor-related apoptosis-inducing ligand receptors death receptor (DR) 4 and DR5 are potential targets for antibody-based cancer therapy. Activation of the proapoptotic DR5 in various cancer cells triggers the extrinsic and/or intrinsic pathway of apoptosis. It has been shown that there are several functional domains in the DR5 extracellular domain. The cysteine-rich domains of DR5 have a conservative role in tumor necrosis factor-related apoptosis-inducing ligand-DR5-mediated apoptosis, and the pre-ligand assembly domain within the N1-cap contributes to the ligand-independent formation of receptor complexes. However, the role of the N-terminal region (NTR) preceding the N1-cap of DR5 remains unclear. In this study, we demonstrate that NTR could mediate DR5 activation that transmits an apoptotic signal when bound to a specific agonistic monoclonal antibody. A novel epitope in the NTR of DR5 was identified by peptide array. Antibodies against the antigenic determinant showed high affinities for DR5 and triggered caspase activation in a time-dependent manner, suggesting the NTR of DR5 might function as a potential death-inducing region. Moreover, permutation analysis showed that Leu(6) was pivotal for the interaction of DR5 and the agonistic antibody. Synthetic wild-type epitopes eliminated the cytotoxicity of all three agonistic monoclonal antibodies, AD5-10, Adie-1, and Adie-2. These results indicate that the NTR of DR5 could be a potential target site for the development of new strategies for cancer immunotherapy. Also, our findings expand the current knowledge about DR5 extracellular functional domains and provide insights into the mechanism of DR5-mediated cell death.Journal of Biological Chemistry 03/2010; 285(12):8953-66. DOI:10.1074/jbc.M109.070680 · 4.60 Impact Factor
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ABSTRACT: In the present article, we report that DR4 or DR5 overexpression dramatically activates the release of the inflammatory cytokines IL-8, TNF-alpha, CCL20, MIP-2 and MIP-1beta in an NF-kappaB-dependent manner in 293T, MDA-MB-231 and HCT-116 cells. We showed that death receptor-mediated signals were extracellular domain-independent, whereas the effect of overexpression of the DR4 intracellular domain was much less potent. The TRADD-TRAF2-NIK-IKKalpha/beta signaling cascade, which plays an essential role in TNF-induced NF-kappaB activation, was found to be involved in tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) receptor-mediated signal transduction. The FADD-caspase signaling pathway, which has been reported to be mostly related to apoptosis, was identified as being essential for DR4 or DR5 overexpression-mediated NF-kappaB activation and cytokine secretion and crosstalks with the TRADD-TRAF2-NIK-IKKalpha/beta signaling cascade. Furthermore, a DR5 agonistic antibody (AD5-10) triggered the inflammatory cytokine release. These data, together with previous reports, provide strong evidence that TRAIL and TRAIL receptors play an important role in inflammation.Cell Research 06/2009; 19(6):758-67. DOI:10.1038/cr.2009.57 · 11.98 Impact Factor
Article: Death Receptor 5-Recruited Raft Components Contributes to the Sensitivity of Jurkat Leukemia Cell Lines to TRAIL-induced Cell Death[Show abstract] [Hide abstract]
ABSTRACT: In the present study we demonstrated Jurkat leukemia cell lines of TIB152 and TIB153 with different sensitivities to recombinant soluble TRAIL cytotoxicity. TRAIL receptor death receptor 5 (DR5) was constitutively localized in the rafts in both cell lines. FADD, caspase-8, and PI3K-p85 subunit were recruited into DR5 lipid rafts of TIB152 but not in TIB153 cells. The expression and enzyme activity of acid sphingomyelinase, which digests sphingomyeline to produce ceramide and plays an essential role in lipid raft assembling, were higher in the rafts of TIB152 than in TIB153. These data provide evidences that DR5-recruited raft components contribute to the different sensitivity of Jurkat leukemia cell lines to TRAIL-induced cell death and may throw some light on the development of better therapeutic strategies for the cancer cells resistant to TRAIL treatment.International Union of Biochemistry and Molecular Biology Life 03/2009; 61(3):261-7. DOI:10.1002/iub.166 · 2.76 Impact Factor
Article: Knockdown of c-FLIPL enhanced AD5-10 anti-death receptor 5 monoclonal antibody-induced apoptosis in human lung cancer cells[Show abstract] [Hide abstract]
ABSTRACT: It is reported that the agonistic antibodies against death receptors 4 and 5 (DR4, DR5) are cytotoxic to various cancer cells. In the present study, the sensitivity of five human lung cancer cell lines to previously reported AD5-10 agonistic antibody against DR5 were investigated. Of these cell lines, A549 and small cell lung cancer showed a moderate sensitivity to AD5-10 and three other cell lines were resistant. Cell line H460 is resistant to AD5-10 despite a high level of cell-surface DR5 expression. We demonstrated that the resistance of H460 cells to AD5-10 was not related to the expression level of DR5, but the expression and cleavage of c-FLIP(L) in the cells. Inhibition of endogenous c-FLIP(L) expression by siRNA significantly enhanced AD5-10-induced cell death in these lung cancer cells. We further showed that this sensitizing effect was associated with decreased expression of Bcl-2 family proteins Bid and Bcl-X(L), change of mitochondrial membrane potential, release of cytochrome c from mitochondria, and caspase activation. Therefore, these data provide evidence that c-FLIP(L) is involved in the resistance of lung cancer cells to AD5-10-induced apoptosis. Moreover, immunohistochemistry on paraffin-embedded tissue revealed that c-FLIP(L) was expressed in 87.9% (29 of 33) of lung carcinoma tissues from the patients, but little in tissues from normal controls. This suggests that inhibition of c-FLIP(L) expression might be a potential strategy for lung cancer therapy, especially for those lung cancers resistant to the agonistic antibody against death receptors.Cancer Science 03/2009; 100(5):940-7. DOI:10.1111/j.1349-7006.2009.01119.x · 3.53 Impact Factor
Article: Tumour necrosis factor‐related apoptosis‐inducing ligand (TRAIL)‐induced chemokine release in both TRAIL‐resistant and TRAIL‐sensitive cells via nuclear factor kappa B[Show abstract] [Hide abstract]
ABSTRACT: Tumour necrosis factor-related apoptosis-inducing ligand (TRAIL) induces apoptosis in a variety of tumour cells, but not in most normal cells, and has attracted considerable attention for its potential use in cancer therapy. Recently, increasing evidence has shown that TRAIL is involved in inflammation, although much of this evidence is controversial. In this article, it is shown that TRAIL induces CXCL2, CCL4 and CCL20 secretion in a nuclear factor kappa B-dependent manner. The dominant negative constructs of tumour necrosis factor receptor-associated death domain protein (TRADD) and tumour necrosis factor receptor-associated factor 2 are unable to block TRAIL-induced chemokine up-regulation, and the dominant negative construct of TRADD may even enhance TRAIL-triggered signals. Using small interfering RNA, receptor interacting protein has been demonstrated to be essential for TRAIL-induced chemokine release. Furthermore, it has been demonstrated that p38 mitogen-activated protein kinase is involved in TRAIL-induced chemokine release without any effects on nuclear factor kappa B activation, suggesting that some unknown transcription factors may be activated by TRAIL. Using a xenograft tumour model, it has been illustrated that TRAIL can also induce chemokine release in vivo. Although these chemokines induced by TRAIL are inflammatory chemokines, their functions are not restricted to inflammation and require further examination. Our results indicate that attention should be paid to the side-effects of TRAIL treatment, not only in TRAIL-resistant but also in TRAIL-sensitive tumour cells.FEBS Journal 02/2009; 276(2):581-93. DOI:10.1111/j.1742-4658.2008.06809.x · 3.99 Impact Factor
Article: AAV-mediated TRAIL gene expression driven by hTERT promoter suppressed human hepatocellular carcinoma growth in mice.[Show abstract] [Hide abstract]
ABSTRACT: A major obstacle in the development of effective recombinant adeno-associated virus (rAAV) mediated gene therapy is infection specificity and gene targeting. In the present study, we investigated whether the human telomerase reverse transcriptase (hTERT) promoter could drive tumor-specific expression of the tumor necrosis factor-related apoptosis-inducing ligand (TRAIL), an apoptosis apoptosis-inducing protein with potential toxic effects on normal cells. Our data demonstrated that hTERT promoter-driven tumor-specific expression of TRAIL decreased the cellular viability of tumor cells, but not normal cells. TRAIL expression driven by hTERT promoter inhibited tumor growth significantly in vivo and combination of viral infection with 5-fluorouracil (5-Fu) suppressed tumor growth more efficiently. Intra-venous injection of virus showed that the recombinant virus was predominantly distributed in the liver, but not in other major tissues tested, and no transgene expression was detected in the liver. Furthermore, serum enzyme and liver histology analysis confirmed that liver function is unaffected by TRAIL expression, significant as the liver is frequently metastasized and scattered with tumors from other organs, which are unpractical to treat by intra-tumor injection. Together our results demonstrate that rAAV-mediated TRAIL expression is a promising strategy in gene therapy for treatment of cancer.Life Sciences 07/2008; 82(23-24):1154-61. DOI:10.1016/j.lfs.2008.03.023 · 2.30 Impact Factor
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Peking Union Medical College HospitalPeping, Beijing, China
Tsinghua University
Peping, Beijing, China
- Department of Basic Medical Sciences
Chinese Academy of Medical Sciences
Peping, Beijing, China
- Institute of Basic Medical Sciences (IBMS)


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"King of the Video Essays" - The New York Times. "One of the brightest film critics working today" - Roger Ebert. "Why doesn't he get a real job?" - Mom. Founding editor and chief video essayist for Fandor's Keyframe. http:www.fandor.com/keyframe Most videos…
NYC SALT New York City
NYC SALT is a non-profit photography program serving teenagers in Washington Heights and the East Village in NYC. Our mission is to engage, inspire and empower teens through professionally led after-school classes that not only teach the art of photography, but also the business. We help our students…
Kirby Ferguson PRO Portland, Oregon
I made the video series Everything is a Remix and am currently producing a new one called This is Not a Conspiracy Theory. If you'd like to support my work, you can do so here: http://everythingisaremix.info/donate/ And you can send cryptocoins to these addresses: Bitcoin: 17myfkig9gaDHS8HcnUgjwkHntyRohUuZb Litecoin:…
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Writer, researcher, educator, videographer, creator and curator/editor of the FILM STUDIES FOR FREE and FILMANALYTICAL websites, and of the AUDIOVISUALCY group here at Vimeo. Also, Senior Lecturer in Film Studies at the University of Sussex, UK, where she is Managing Editor of the new open access digital…
I like expensive things. http://twitter.com/apocalypstick
Daniel Canogar Plus Madrid
Specialized in the expanded field of photography and video, creating new media installations, photo-murals and site-specific public art projects. Interest in themes related to electronic waste, visual excess and the archeology of new media.
Chris Bors New York, NY
Chris Bors lives and works in Harlem in New York City. He received his MFA from School of Visual Arts and also studied at Rhode Island School of Design. His art has been exhibited at PS1 MoMA, White Columns, Sixtyseven and Ten in One Gallery in New York, Casino Luxembourg in Luxembourg, and the Videoex…
Patrice De Bruyne Belgium
Born in 1949 . Architect and designer (hi-tech syle). Former editor of many magazines, including Chrome & Flames (Europe), Top Wheels (USA & UK) published from 1979 to 1999 (500.000 copies per month) ... My hobbies are to take care and drive during the weekend some American cars of collection,…
Olivia Hayes Raleigh, NC
Self-expression junkie. New media debutante.
Isaac Niemand São Paulo
ARS LONGA, VITA BREVIS www.bossanovafilms.com.br REEL https://vimeo.com/20191290 SITE http://isaacniemand.tumblr.com/
Leandro Copperfield Rio de Janeiro, Brazil
Just a film lover. firstname.lastname@example.com http://leandrocopperfield.blogspot.com
Lucas McNelly Plus nowhere/everywhere
Lucas McNelly is the award-winning filmmaker behind such feel-good movies as A YEAR WITHOUT RENT, UP COUNTRY, BLANC DE BLANC and GRAVIDA. His work has been featured all over the place. Maybe you've heard of him, maybe you haven't.
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Published June 29, 2012
Long Beach, CA 562.628.1007 www.obagi.com Sales: $114 million. Net income: $10 million. Key Personnel: Albert F. Hummel, president, director and chief executive officer; Preston S. Romm, chief financial officer and executive vice president of financ… Read More »
Published June 29, 2012
Oak Brook,IL 800.837.1800 www.blistex.com Sales: $125 million (estimated). Key Personnel: David C. Arch, chairman and chief executive officer; Mike Donnantuono, president. Major Products: HBA brands include Blistex, Stridex, Glysomed and Odor-Eater… Read More »
Published June 29, 2012
Akron, OH 330.255.6000 www.gojo.com Sales: $133 million (estimated). Key Personnel: Joe Kanfer, chairman and chief executive officer; Scott Levin, chief financial officer and chief administrative vice president; Harold Tyreman, vice president of sal… Read More »
Published June 29, 2012
Los Angeles, CA 310.410.9600 www.herbalife.com Sales: $147 million for personal care products. Corporate sales: $3.4 billion. Key Personnel: Michael O. Johnson, chairman and chief executive officer and director; Brett R. Chapman, general counsel; De… Read More »
Published June 29, 2012
A Division of ACMG Jersey City, NJ 800.672.2259 www.atlanticcoastmedia.com Sales: $156 million. Key Personnel: Thomas Shipley and Andrew Surwilo, co-founders and chief executive officers; Darek Hrynkiewicz, chief of operations; John Ende, chief fina… Read More »
Published June 29, 2012
Burlington, VT 802.658.3773 www.seventhgeneration.com Sales: $165 million. Key Personnel: John Replogle, chief executive officer and president; Tim Fowler, chief scientific officer; Joey Bergstein, chief marketing officer. Major Products: Natural 4… Read More »
Published June 29, 2012
El Paso, TX 915.225.8000 www.hotus.com Sales: $200 million (estimated) for personal care products. Corporate sales: $1.1 billion. Net income: $110.3 million for the year ended March 31, 2012. Key Personnel: GeraldJ.Rubin, chairman, chief executive o… Read More »
Published June 29, 2012
Los Angeles, CA 323.728.2999 www.aiibeauty.com Sales: $205 million (estimated). Key Personnel: Zvi Ryzman, president; Terri Cooper, executive vice president; David Woolf, executive vice president of retail sales; Mark Moesta, vice president of profe… Read More »
Published June 29, 2012
White Plains, NY 800.873.7400 www.combe.com Sales: $250 million (estimated). Key Personnel: Christopher B. Combe, chairman and chief executive officer; Doug McGraime, president and chief operating officer Major Products: Men's Grooming—… Read More »
Published June 29, 2012
Irvington,NY 914.524.6800 www.prestigebrands.com Sales: $344 million (estimated) for personal care and household products. Corporate sales: $441.1 million for the year ended March 31, 2012. Key Personnel: MatthewM.Mannelly, president and CEO; RonLom… Read More »
Published June 28, 2012
A Sanofi Company Chattanooga, TN 800.366.6077 www.chattem.com Sales: $352 million BullFrog Mosquito Coast SPF 30 Key Personnel: Zan Guerry, chairman of the board and CEO of Chattem Inc. at Sanofi-Aventis US; John Stroud, exe… Read More »
Published June 28, 2012
St. Louis, MO 314.985.2000 www.energizer.com Sales: $419 million (estimated) for sun care, skin care and shaving products. Corporate sales: $4.6 billion. Net income: $261 million for the year ended Sept. 30, 2011. Key Personnel: Ward M. Klein, chief… Read More »
Published June 28, 2012
Meridian, ID 208.472.0800 www.scentsy.com Sales: $535 million. Key Personnel: Orville Thompson, chief executive officer; Heidi Thompson, president. Major Products: Home fragrances including scented wickless candles and personal fragrance products s… Read More »
Published June 28, 2012
Whitehouse Station, NJ 908.298.4000 www.merck.com Sales: $583 million for foot, skin and sun care products; corporate sales: $48 billion. Net income: $6.2 billion. Key Personnel: Kenneth C. Frazier, chairman, president and chief executive officer; C… Read More »
Published June 28, 2012
Westlake Village, CA 805.449.3000 www.jafra.com Sales: $611 million. Key Personnel: Dr. Friedrich Kroos, president and chief executive officer, Jafra Cosmetics International; Hans ter Pelle, senior vice president and chief financial officer; Mari Lo… Read More »


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Hedge fund executives are heading south, and not just for the winter. The New York Post reports high-finance firms are leaving the high-tax environment of New York City for the lower taxes of Florida.
While the Sunshine State has no state income tax, the state and local taxes in New York amount to nearly 15 percent.
Business property is also much cheaper in Florida than New York.
Meanwhile, Florida is welcoming their new residents. Palm Beach has set up an office to answer questions from New York hedge funds looking to move to the area.
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Are you seeking answers in life? Are you hurting? Are you facing a difficult situation?
Find peace with God, discover more about God or send us your prayer request.
Call The 700 Club Prayer Center at 1 (800) 823-6053, 24 hours a day.
A caring friend will be there to pray with you in your time of need.
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This Fourth of July weekend we're taking a look at some of the stories the highlight our nation's history. CBN News also brings the heroic stories of men and women who keep America safe by serving in the armed forces.
The Fairfax County Public school board recently approved lessons about homosexuality and gender identity in its Family Life Education curriculum. The move has angered many parents who are concerned they cannot opt their children out.
An Oregon official certified a ruling Thursday imposing a $135,000 fine against Christian bakers who refused to make a wedding cake for a lesbian couple.


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Cover Stories
Practice Mgmt.
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Press Releases:
ChiroTouch to Host "Transform Your Practice in One Simple and Surprising Step" Webinar with Dr. Douglas Sea and Dr. Nathan Unruh
ChiroTouch Announces ICD-10 Readiness Online Quiz for Chiropractors
Flambeau Inc., Cultivate Sign Agreement for On-Site Corporate Wellness Services
Annual NYCC Employee Recognition Luncheon Held at Gould Hotel
Performance Health Launches Enhanced Research and Exercise Website
Foot Levelers Welcomes Two New Seminar Speakers
Dr. George B. Curry Becomes 18th President of ICA
Kareo and Medicfusion Partner to Support Chiropractic Practices
ACASC Chiropractors Attend to Medical Needs of Nearly 600 Athletes at 40th National Collegiate Taekwondo Championships
Douglas Laboratories® Launches New Line of Vision Health Supplements
UWS student, Freya Moran, selected as first chiropractic participant in the LEAPS into Integrative Medicine program
Dr. Stuart E. Hoffman Honored as ICA's Chiropractor of the Year for 2015
"Golden Heart Award" 2015 from Oklahaven Children's Chiropractic Center
University of Bridgeport, College of Chiropractic Names New Dean Michael A. Ciolfi HBPE, DC, MBA, DBA
Logan University 2015 Board of Trustees Announced
Press Release
Klean Athlete® Introduces KLEAN OMEGA™ NSF Certified for Sport® Fish Oil
Press Release
Written by TAC Staff
Sunday, 15 December 2013 22:42
Unique high-potency triglyceride supplement in a sea of fish oil products aimed at athletes
PITTSBURGH, Pa., December 3, 2013 –
Klean Athlete®
, a line
NSF Certified for Sport®
nutritional supplements for athletes announces today that it is expanding its product offering with the addition of KLEAN OMEGA™, a supplement designed to provide essential omega-3 fatty acids from clean, pure fish oil to support the cardiovascular, neurological and joint health of athletes who want to reach peak performance through optimal health.†
"KLEAN OMEGA™ is an essential addition to the Klean Athlete® line," says Andrew Halpner, PhD, Head of Klean Athlete R&D. "Klean Omega™ is one of the very few fish oil products on the market that is NSF Certified for Sport®, and is to our knowledge the most potent Triglyceride EPA and DHA sport formula available in a convenient single soft gel." Unlike most other fish oils, KLEAN OMEGA™ is also a bovine-free product by using a unique fish oil gelatin soft gel from tilapia.
KLEAN OMEGA™ high-potency fish oil may assist in the normal recovery of inflammation after vigorous exercise, promote healthy joints, and support proper breathing and lung capacity in active athletes."†
In a study published in the Journal of the International Society of Sports Nutrition, researchers found that participants taking fish oil for six weeks increased lean muscle mass and reduced fat mass. In other studies, omega-3 fatty acids have also been shown to promote better oxygen delivery during exercise, help remove lactic acid from the body, and improve reaction time in athletes by supporting the central nervous system.†
Like the rest of the Klean Athlete® line of products, KLEAN OMEGA™ recently underwent a meticulous testing and verification process to receive the esteemed NSF Certified for Sport® certification, which ensures that it is free of banned substances. Recognized by major sports organizations including the NFL, MLB, NHL and PGA, the NSF Certified for Sport® program was developed in response to the concerns of athletes, coaches, team owners and others about finding banned substances in sports nutrition products.
KLEAN OMEGA™ joins the existing eight Klean Athlete® supplements including:
Klean Multivitamin™
Klean Antioxidants™
Klean Cognitive™
Klean Probiotic™
Klean Isolate™
Klean Electrolytes™
Klean Endurance™
Douglas Laboratories® maintains outstanding quality control from start-to-finish for Klean Athlete® ingredients and products. They are one of the few supplement providers who control manufacturing, packaging and distribution, thus ensuring that the highest quality product reaches every athlete.
To learn more about KLEAN OMEGA™ and the full line of Klean Athlete® nutritional supplements, please visit
† These statements have not been evaluated by the FDA. These products are not intended to diagnose, treat, cure or prevent any disease.
Klean Athlete®
NSF Certified for Sport® supplements were designed to maintain the healthy lifestyle and promote peak performance of athletes, from the weekend warrior to the amateur and professional competitor. This line of certified nutritional supplements is backed by more than 50 years of science, dedicated research and manufacturing excellence. Klean Athlete® will be available through licensed healthcare professionals as well as qualified coaches, trainers, and registered customers at
Klean Athlete® is tested and certified by the NSF Certified for Sport® program (
) part of
NSF International's Health Sciences Division
This growing division offers training and education, consulting, GMP and GLP testing, certification, R&D and auditing for the dietary supplement, pharmaceutical and medical device industries throughout the entire product lifecycle. The Health Sciences Division includes NSF's prestigious Dietary Supplements Certification Program. NSF International developed the only American National Standard for Dietary Supplements (NSF/ANSI 173) and tests and certifies supplements against this standard. NSF's Health Science Division operates globally throughout North America, Europe, the Middle East, Africa, Asia and Latin America. For more information visit
Atrium Innovations Inc.
(TSX: ATB) is a globally-recognized leader in the development, manufacturing, and commercialization of innovative, science-based natural health products which are distributed in more than 35 countries. The Company owns healthcare practitioner and specialized retail product brands that are at the forefront of science, innovation and education. Atrium has over 1,300 employees and operates seven manufacturing facilities with current Good Manufacturing Practices. Additional information is available at
New York Chiropractic College: Affiliated with VA Residencies at Two of Five Selected VA Facilities
Press Release
Written by Kathy Hadley
Thursday, 12 December 2013 15:31
Seneca Falls: New York Chiropractic College (NYCC) will soon participate in the Department of Veterans Affairs new full-time, year-round, paid chiropractic residency program slated to begin this coming July. Administered nationally by VA's Office of Academic Affiliations and locally by the individual VA facility, the residency programs will enjoy the benefit of a VA facility affiliation with Council on Chiropractic Education (CCE) accredited chiropractic schools.
Focusing on Integrated Clinical Practice, and emphasizing the provision of chiropractic care in an integrated health care system, NYCC's VA-residents will hone vital clinical skills as senior VA-chiropractors mentor the hands-on training in Canandaigua and Buffalo, N.Y. – two of only five sites to be awarded such residencies nationwide. Drawing annual salaries ranging between $36,000 and $43,000 (based on geographic location) the residents will carry out clinical educational rotations through such services as primary care, medical or surgical specialties, mental health, and rehabilitation disciplines. The residents will also participate in a variety of scholarly activities, giving and attending academic and research presentations at the VA facility and its affiliates.
NYCC adjunct faculty member Dr. Paul Dougherty provided oversight and instruction while gathering data on the effectiveness of a pilot residency. Drs. Paul Dougherty and Andy Dunn will mentor residents under the new program, ensuring quality patient care and clinical education in the unique environment.
Medicfusion Partnership with MD On-Line Offers Major Benefits for Chiropractors
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Press Release
Written by Terri Sundholm
Thursday, 12 December 2013 13:32
RALEIGH, NC – Medicfusion EHR today announced a partnership with MD On-Line, Inc. (MDOL), a leading provider of electronic healthcare solutions that leverage data to improve practice efficiency and enhance patient care. This partnership gives the chiropractic community one-stop access to a total electronic health records (EHR) solution by combining HITECH Certified Medicfusion EHR with the powerful financial management and billing capabilities of MDOL's InSync® Practice Management (PM) solution.
Called ChiroComplete, the combined product suite equips chiropractors with an EHR system designed to meet the challenges ahead in healthcare, such as the increase in government regulations surrounding documentation and compliance.
"This is a win-win partnership for chiropractors. In one complete package, they receive unsurpassed EHR automation and efficiency, as well as one of our industry's most advanced financial management systems," said Medicfusion CEO Shayne Sundholm.
A cloud-based system designed by chiropractors, Medicfusion increases office efficiency, simplifies documentation and compliance, and improves the speed and accuracy of SOAP notes. The system is also HITECH-certified, a major consideration since incentive dollars are available to practices that implement a HITECH-certified EHR.
MDOL's InSync® Practice Management (PM) solution maximizes productivity and revenues streams by reducing operational costs and increasing profitability. The company's fully automated billing system delivers exciting, new levels of efficiency when teamed with Medicfusion.
"In today's rapidly changing healthcare environment, an EHR system with seamless billing and financial reporting capabilities is critical to survival for chiropractors," said MDOL EVP Ashish Vachhani. "There's no better time to embrace this technology than right now."
Specific benefits to chiropractors from Medicfusion include an easy-to-use paperless system that fully automates your front office. Key features include Clinical Documentation (SOAP Notes), Transcription Library, Patient Portal Website, Custom Forms Library and ePrescribe.
With MDOL's InSync® product, chiropractors benefit from effective management of payments and detailed financial and analytical reporting. Highlights include intuitive electronic remittance advice, electronic super bill creation module, charges generated at the time of charting, easy-to-review accounts receivable reports, daily reconciliation reports, and monitoring of outstanding balances and payments based on insurance carriers or patients.
The official release of ChiroComplete is expected mid-January 2014 with presales on now.
For more information about the ChiroComplete solution, contact Medicfusion at 1-866-643-7778,
20/20 Imaging Brings Innovations in Digital X-Ray and Imaging Software To RSNA 2013
Press Release
Written by Brittny Quinn
Thursday, 12 December 2013 13:30
RSNA BOOTH #8516 – November 21, 2013 - 20/20 Imaging, a leader in small market imaging with nearly 2,500 installations across the country, announced today their product line-up for RSNA 2013, led by a new digital x-ray system with enhanced software capabilities and higher frequency generator. Targeted more specifically to the podiatry market, this higher-frequency system offers the potential for lower dose with advanced imaging capabilities that enable lower technique for lower radiation to be used. The system is also anatomically programmed – a one-of-a-kind capability for podiatry practices – saving time, assuring accuracy, and further enhancing efficiency by enabling exams to be selected at the technologist workstation instead of at the device.
"As we continue to grow our imaging product portfolio, bringing our products to RSNA and being part of the ever-growing imaging community is a strategic business imperative for us," explained Bob Salzman, president, 20/20 Imaging. "We are excited to showcase our most advanced portfolio to date along with our integrated software capabilities that paint the future of streamlined workflow in small market imaging."
Beyond podiatry, 20/20 Imaging's product portfolio also includes solutions for the chiropractic, hand and now the veterinary markets as well.
Advanced, complete end-to-end imaging solutions for chiropractic practices include the cutting-edge Chiro Straight-Arm digital x-ray solution, equipped with the company's CFPG and the industry-leading C-DR retrofit system, both enable the direct capture of images and quick display of data on computer workstations within seconds.
The HDR & HFP, dedicated specifically to the unique needs of the hand surgery market, were unveiled at this past October's American Society for Surgery of the Hand (ASSH) Annual Meeting.
After years as a leading provider of custom retrofit solutions for veterinary markets, the addition of the V-XRS, a multi option digital receptor turn-key x-ray system equipped with a 32kW HF APR generator, 4-way float top table and mini-PACS will further advance the digital imaging capabilities for veterinary practices.
20/20 Imaging works closely with sister company Viztek, Inc., a leading provider of complete digital software and hardware diagnostic imaging solutions, and also an RSNA exhibitor with their full portfolio of products and solutions for imaging centers and hospitals.
About 20/20 Imaging:
20/20 Imaging is a value added reseller of HealthCare IT, including digital imaging solutions tailored for the private practice market space, specializing in podiatry and chiropractic. Utilizing its broad HealthCare IT and imaging expertise and new economical DR, CR and PACS solutions, 20/20 Imaging provides comprehensive digital imaging & IT solutions and services to a large variety of small to medium size clinical specialties.
Top Chiropractor in Atlanta First Choice for Celebs
Press Release
Written by Century Center Chiropractic
Monday, 14 October 2013 13:47
ATLANTA, Oct. 13, 2013 (GLOBE NEWSWIRE) -- The top chiropractor in Atlanta, Dr. Jared Simon, fixes backs; but he's no ordinary chiropractor. Better known as the "chiropractor to the stars" among the Atlanta area, Dr. Simon is the first choice among both local and major celebrities when it comes to relieving back and neck ailments of all kinds. In fact, his list of star clients reads like a red carpet roll call.
Despite being widely considered the top chiropractor in Atlanta, Dr. Simon remains surprisingly modest when asked about his work with superstar Cee Lo Green, Olympian Gwen Torrence or NFL Quarterback Joe Theismann. He explains:
"I specialize in the entertainment industry, but my main concern is making sure my patients live pain-free, whether the person I'm working on is a Grammy winning artist, a teacher, an Olympian, a car mechanic, a sports legend or an accountant. It's not what they do that matters so much to me; it's how I can get them out of pain and keep them out of pain that drives how I care for them."
Despite, or perhaps because of, his down-to-earth nature, Dr. Simon is becoming a celebrity in his own right. Once word got around that the top chiropractor in Atlanta had so many high-level clients, people wanted to know more, and he has now appeared on television and radio programs as an expert on chiropractic care.
Dr. Simon not only specializes in caring for those in the entertainment industry, he is also the founder of the Atlanta Actor's Healthnet, which features physicians sharing ideas on healing back pain for those in the performing arts.
While he is laid back about his celebrity clientele, he also realizes the importance of paying attention to people's line of work and how that affects their back and neck issues. "My patients' professions definitely impact their back problems," Dr. Simon says, "from repetitive stress injuries, which can be seen among athletes, to people suffering from pain brought on by a sedentary lifestyle, what they do or don't do during the day can inform their level of back issues." Dr. Simon says he does a careful assessment of these concerns and customizes a plan for each patient, depending on their individual needs and comfort levels.
Dr. Simon has become so well-known in the Atlanta entertainment industry that he is now the go-to Atlanta chiropractor for celebrities. He is also a member of the Screen Actors Guild, making him very familiar with the special concerns of film and stage actors.
His practice, Century Center Chiropractic, is located Atlanta, Georgia. They offer state of the art equipment and a personalized approach to back pain. Each patient is thoroughly assessed and a range of treatment options is offered. Dr. Simon has been practicing chiropractic care since 1992.
CONTACT: Century Center Chiropractic 888-667-5235
More Articles...
Palmdale Chiropractor Helps Golfers Improve Their Game
Stay Fit Seniors®: As U.S. 'Health Crisis' Worsens, Life-Changing New Book Empowers Seniors to Exercise, Eat Well & Prosper
Kinesio Marks Breast Cancer Awareness Month
"Thought Leader", Dr. Matt Hamilton Is Not Your Parent's Chiropractor!
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Griffeys, Fielders Among Top Father-Son Combos In MLB History (Photos)
As young kids, many children dream of following in their fathers' footsteps. Especially if their fathers are really, really good at baseball. The game of baseball always has been a family affair, as fathers pass their passion to their sons by playing catch in the yard or watching games on TV together. In a few special cases, though, the two generations have taken that love to...
Ken Griffey Jr. Photographs Son's Fiesta Bowl Game With Enormous Camera (Photo)
Ken Griffey Jr. has found himself a new hobby. The former baseball star (looking noticeably larger) has become quite the photographer by the looks of it, as he spent New Year's Eve playing shutterbug during his son's college football game in Glendale, Ariz. ICYMI: Ken Griffey, Jr. was just out here taking photos at the #FiestaBowl. pac12.me/14dECyb http://t.co/5737Ev3lqg�...
Ken Griffey Jr. disliked Yankees because of Billy Martin
Ken Griffey Jr. never considered going to the New York Yankees during his Hall of Fame career, and the reason is pretty unforgettable. Ken Griffey Sr. joined "Boomer & Carton" on WFAN in New York Wednesday to talk shop with the boys, and he dished on his son's problems with the Yankees. According to Griffey […]
Via Larry Brown Sports
July 17, 2014 | Discuss
The Dugout: Ken Griffey Jr. really despises Linda Cohn
Via uproxx.com
Ken Griffey Jr. apologized to Linda Cohn for trainwreck interview
Yesterday former MLB superstar Ken Griffey Jr. went on SportsCenter and gave a brutal, lifeless, deadpan, trainwreck of an interview to anchor Linda Cohn. As much as Cohn tried, she couldn't get more than one word out of Griffey on more than one occasion. Shortly after the interview aired, and after a mountain of negative publicity, Griffey posted this message on Instagram...
Via Awful Announcing
Ken Griffey Jr. issues apology to Linda Cohn for awkward interview
Less than 24 hours after giving one of the most awkward interviews of all time, Ken Griffey Jr. issued an apology to Linda Cohn.
WATCH: Ken Griffey Jr.'s painfully awkward interview on SportsCenter
God bless Linda Cohn. She tried as best as she could to get a decent interview out of Ken Griffey Jr. but he was clearly in another universe. Griffey appeared on the afternoon live SportsCenter in a promo spot for Upper Deck and Cohn asked him questions about his favorite cards growing up and thoughts on today's MLB. Griffey's responses were... less than forthcoming. The...
Via Awful Announcing
March 05, 2014 | Discuss
Ken Griffey Jr. owns 100 copies of Photoshopped Upper Deck rookie card (photo)
Ken Griffey Jr.'s arrival in the major leagues in 1989 as a bright-eyed, teenaged rookie with the Seattle Mariners was met with great fanfare and anticipation, not only from baseball fans, but those involved in the baseball card business as well. Griffey was only 18 years old when his Upper Deck rookie card was released. […]The post Ken Griffey Jr. owns 100 copies of his Photoshopped...
Via Sportress of Blogitude
February 06, 2014 | 1 Comment
Reds reveal Ken Griffey Jr. bobblehead night
The Reds revealed another bobblehead from this season's promotional schedule on their official Twitter account Wednesday afternoon. To celebrate his induction into the Reds Hall Of Fame, Saturday June 21 will be Ken Griffey Jr. bobblehead night at Great American Ball Park. The Reds Hall of Fame Induction Weekend will take place August 8-10, and will honor the storied careers of Ken...
Via Fox Sports Ohio
February 05, 2014 | Discuss
Ken Griffey Jr. photographs son scoring first college touchdown at Advocare V100 Bowl (GIF/VIDEO)
Yes, that was Ken Griffey Jr. wielding a camera with the 600mm lens on the sidelines of the Advocare V100 Bowl Tuesday.Griffey, who recently joined Instagram, has been sharing a fair number of photos through Twitter and was on the Independence Stadium field as a credentialed media member for ESPN.Hopefully, the former Seattle Mariners great snapped some nice shots for ESPN, but...
Via The Other Paper
December 31, 2013 | Discuss
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Saturday Night at the Brewster Meeting House
June 13, 2015 7 pm
The First Parish Brewster "Players" will entertain with song, dance and performance art with instrumental accompaniment by our musicians along with pianist, Lucy Banner.
Please join us to celebrate life!
Tickets: $15
Benefit to help pay down the mortgage for the restoration of our beautiful historic meeting house.
Handicapped Accesible Parking Across the Street
Open Mic Classical
Are you a doctor with a classical horn habit, or an orchestra member who misses playing in small ensembles? Are you an aspiring young student who doesn't ever get a chance to play with a pianist, but would like to develop your performing skills? Or a composer eager to try out your newest work?
Dust off your flugelhorn, oil your keys, and come share your talent in an intimate and casual atmosphere.
Admission is free, and classical musicians of all ages and levels are welcome. Pianist on site is available to accompany you.
"Open Mic Classical" is brought to you by clarinetist Mónika Woods (winner of the Cape Symphony's 2013 Soloist Competition) and amateur violist and sculptor Robert Marcus representing First Parish Brewster, with Lucy Banner and Elizabeth Tipton as collaborative pianists.
WHEN: 3rd Sunday of the Month
March through June
Next one: June 21th, 2015, 3 pm
(sign up at: 2:45pm)
Featuring: Bruce Abbott, admired and well-known Cape musician. Although primarily known as a stunning jazz player, Bruce also has a love for classical music, especially (ancient jazzer) J. S. Bach.
WHERE: Brewster Meeting House (First Parish Church, 1969 Main Street or Rt. 6A, Brewster, MA)
Admission: FREE (donations welcome to support this continued success of this event)
Whether you are an amateur musician, performing artist, fan of classical music, volunteer, venue or business seeking to sponsor a cause, please contact us.
For more information please contact Mónika Woods (web: klarinetista.com Ph: (774)-353-0244), or Bob Marcus (Ph: (508)-945-0832 ).
Join us this summer in the historic
179-year old Brewster Meeting House
for a morning of soaring melodies
and rubber chickens.
Enjoy performances by 2 of the
Cape's most dynamic entertainers
in this beautifully restored, air-conditioned


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text: The Chinese food at this Ft. Lee standby, in the same location for 25 years, is only average, but what bumps this place up one category is their serving of dim sum daily at lunch. We have eaten here several times over these past 25 years. I went for lunch today with a business associate and the... read more


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Your Eye On Health
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Day 14 - Salt Lake City Go Karting BK
Posted by BKtheKing on Nov. 16, 2010
Break celebrates 4th of July the American Way: by laying the smack down on ...
With the 4th of July around the corner, we celebrate the most kick-ass USA ...
Dude, are you drunk AND high? Or are you just being PRANKED?!
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This restaurant is quirky, fun with fabulous food and is great value for money. Definately the best pizza in town and a great option for vegetarians.
If you own or manage La Jument verte, register now for free tools to enhance your listing, attract new reviews, and respond to reviewers.


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Good Cook Nonstick Utility Knife #18803
Pack the coolers for your next tailgate and don't forget to bring the Good Cook Nonstick Utility Knife for slicing tomatoes grill side. This knife boasts a rubberized grip and comes with a handy blade cover for safe transporting. Although it left some torn edges while slicing in our tests, chances are no one will notice if the tomatoes are tucked into a bun with a juicy burger. Just be prepared to wash the Good Cook by hand when you get home; this utility knife isn't dishwasher safe.
Reviewed: August 2013
Price When Reviewed: $15.69
- Good at slicing tomatoes
- Comfortable to use
- Tomato slices had torn edges in our tests
- Not dishwasher safe
What do you think?
Vacu Vin Garlic Press #6056040
The heavyweight Vacu Vin Garlic Press tore through both peeled and unpeeled garlic, topping the charts in our test.
Wilton Cookie Max Cookie Press #2104-4003
This bargain press quickly stamped out 30 cookies.
Kuhn Rikon Clear Cookie Press #2461CLB
This cookie press comes with a plastic storage box to organize all of the pieces.
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This property allows you to book online with a credit card. After submitting a reservation request, the owner or manager has 24 hours to accept, at which time your card will be charged and you will receive an email confirmation
Recent Higgins Lake reviews
"The Flynn Family Cottage is an ideal place for a large family stay on Higgins Lake (or a smaller family). Actually, in our 30+ years going to Higgins, it is one of the nicest cottages we have stayed in. On this trip there were eight of us; my wife and me, two sons with their wives and two grandchildren. Everyone had a lot of room to move around in without bumping into one another. The cottage layout is great. The very large kitchen is separated from the living room. The living room is spacious with comfortable seating for everyone. There are four bedrooms and two bathrooms. The highlights of this location include a long private entry road through the woods that gave it a north woods feel. The beach and fire pit are great. The lake at this point had a smooth sandy bottom and the grandkids (3 & 5) could walk out beyond the end of the dock and it would only be up to their chest. At night on the dock, the stars were magnificent and you could see the Milky Way. We had a great time and highly recommend the Flynn Family Cottage. We're going back in 2016!!"Guest: Alan Date of Stay: July 2014 Review Submitted: July 31, 2014View listing
"My sons, boyfriend, and I really enjoyed our first stay at Higgins Lake! The lake is beautiful! We brought our boat for tubing and fishing, and had a great time. The views from the cabin were great, and it was so relaxing to sit on the swing, looking out on the lake. The cabin had everything we needed. The updated bathroom was very nice. I would recommend going to the Silver Dollar (can walk to it). Nice people, huge portions."Guest: Anonymous Date of Stay: September 2014 Review Submitted: September 15, 2014View listing
"As soon as we walked in we knew we were home. The open design, tongue and groove stained paneling, way-cute kitchen, the cabinstyle decore (and not over done) endeared us to this retreat on the South Branch of the Au Sable River. Now, if you know anything about the Au Sable, the Holy Waters East of Grayling is the premier fly fishing venue. But if you really know about the Au Sable, the Mason Tract is in fact a great, quiet place to be - fly fishing, kayaking, hiking - quiet being the emphasis. This refreshing retreat is just upstream from the Mason Tract, easy to get to yet wonderfully apart from the bustle of the better know stretches. One regret, that we did not bring our kayaks with us. It would be so easy to launch from their little dock; then you would have miles of the South Branch to float and fish. Or just slip into your waders and venture out right off shore. We know how good the trout fishing is there (foolish of me to admit, but I won't tell all of the secret places... you can discover them for yourself from this property.) The amenities were perfect for what we wanted, the ease of relaxing as if at home yet being in a fun place where fixing a fancy meal was a breeze. Throwing a load of clothes in the washer was more than convenient, plenty of space to hang out, read a book, whatever, or enjoy a cup of coffee at the river's edge while a hatch was going on, with the morning sun dancing through the swirling mayflies. And then there was the outdoor hot tub. After a hard day playing, even in the rain it was the added deliciousness of being at this place. After, to fall asleep on a comfortable bed with utter quiet and a sweet forest view made each night special. Getting up was difficult. Too comfortable. Completely relaxed. Refreshed. It was more difficult to leave. Oh yes, we will be back. Many more memories to yet be made. Thank you Bill and Denise for offering your comfortable place for others to delight in, and for making this easy to rent, quickly responding to our inquiries and more than meeting our needs. You have gone beyond. Tom and Margie St Joe"Guest: Margie and Tom Mattice Date of Stay: May 2015 Review Submitted: May 30, 2015View listing
"We have been looking for several years to find a nice place on the lake that we could take our Lab and relax, and we finally found the right one on Higgins Lake! Nice beach, our dog loved swimming here! We had a nice and relaxing time this week even if the weather was on the cool side. We plan on booking again next year."Guest: Judy M. Date of Stay: June 2015 Review Submitted: June 11, 2015View listing
Featured Destinations: Estes Park Cabins, Cabins in Colorado, Starved Rock Cabins, Wisconsin Dells Cabins


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The Large Hadron Collider is being used to attempt to produce the particle – nicknamed the "God particle" – which scientists believe will help explain how the universe came into being.
Physicists have yet to make a Higgs boson particle, but they now believe that when it is created it will sound like several coins clattering around the bowl of a wine glass.
The team working at the LHC in Switzerland hopes that if and when it is successful in creating a Higgs boson by crashing protons into each other at super fast speeds, it will be easier to detect because they will know what to listen for.
Scientists used information from computer models to calculate what the creation of the particle will sound like, a process called "sonification".
LHC Sound, a group of scientists, musicians and artists in London, has used data on the particles and matched it to qualities such as pitch and volume to determine how the collision will sound.
Dr Lily Asquith, who models data for the LHC and has contributed to the sound project, wrote on her blog: "Sound seems the perfect tool with which to represent the complexity of the data.
"Our ears are superb at locating the source and location of sounds relative to one another ... We also have an incredible ability to notice slight changes in pitch or tempo over time and to recognise patterns in sound after hearing them just once."


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Keep Your Pets Cool This Summer With These Handy Tips
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Your Pup Will Beg For More of This Homemade Apple Crisp Dog Treat


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Handblown pale-green glass vase, from the Biot glass factory in Provence. 1985.


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VIDEO: Star Wars AT-AT comes to life as cute canine
As a kid, the creator of this video always wanted a dog and a Kenner AT-AT, well thanks to a bit of SFX and a liberal sprinkling of talent he's managed to combine the two.
We always try and remain neutral here at Pocket-lint, but anyone who doesn't at least smile at the video above is either heartless, a bounder or both.
So take a look at this great clip by Patrick Boivin, and let us know what you think in the comments below.
At internetmatters.org parents can find all the advice they will need to keep their children safe online. Designed specifically for parents, the site offers a wealth of up-to-date, unbiased information and advice about how to deal with online safety. Parents can learn about the latest issues and technologies, get great tips on how to talk about online safety with their children and get the best advice on dealing with issues and taking action. Created with experts, Internet Matters provides detailed information, but also signposts to best-in-class resources from individual expert organisations. Our goal is to ensure parents can always access the information that they need, in a format that is clear and concise.


text: The . wiki last edited by fesak on 06/25/13 10:52AM View full history From award-winning illustrator and acclaimed comic book artist Nick Percival comes a dark, original fairy tale. You think you know them. Think again. Violently ripped from the pages of folklore come the Enchanted. Supernatural immortals living in a dark, steampunk, creature-infested world where nature, technology and foul magic are in constant conflict. When the twisted, burnt remains of the half-wooden, half-mechanical warrior, Pinocchio, are discovered, wolf hunter Red Hood and giant killer Jack realize the fragile rules of their existence have been shattered. With the help of other powerful Enchanted (the mercenary, Goldilocks and psychic exterminators, Hansel and Gretel), Jack and Red team up on an adventure to stop whatever, or whoever is destroying their powers and murdering their kind.


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With 1.7 million freelancers, we'll match you with the perfect talent.
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Insert a specific Outlook email signature based on the first two letters in the Subject line. A macro that will allow the selection of a specific signature to be added at the end of the email based on the first two letters in the Subjec line.
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As a Modern Apprentice you will work towards a qualification that it related to your job and is recognised by employers. You will also gain valuable work experience at the same time.
The skills you gain during your Modern Apprenticeship will make you an attractive candidate for future employers. It will also help you build your own confidence and personal development.
Modern Apprenticeships are open to anyone aged 16 or over. However, if you're over 25, your employer may have to pay for the cost of the training.
We do not currently have any Modern Apprenticeship opportunities. You can register with myjobscotland and receive job alerts to be the first to know when we are recruiting again.
Scottish Apprenticeship Week showcases the successes of apprentices who have chosen to develop their careers by learning while they work to gain nationally recognised qualifications.


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The Two-Way
6:29 am
Thu March 28, 2013
Judge Rules Pistorius Can Leave South Africa
Originally published on Thu March 28, 2013 8:36 am
Olympic and Paralympic sprinter Oscar Pistorius can travel outside his native South Africa while he awaits trial for allegedly murdering his girlfriend, a judge in Pretoria ruled Thursday.
The Associated Press writes that "Judge Bert Bam says [Pistorius] must provide authorities with his travel plans at least a week before he leaves the country. He must also return his passport to the court within 24 hours of returning to South Africa."
Other restrictions placed on Pistorius when he was granted bail in February were also relaxed. South African's News 24 reports that the judge said Pistorius may visit his home — where Reeva Steenkamp was killed — and that he does not have to report to a local police station twice a week.
According to News 24, Bam said the restrictions placed on the world famous athlete "didn't serve any purpose."
Pistorius did not appear in court Thursday, local news outlets say.
As we've previously written:
The 26-year-old Pistorius, known as the "blade runner" because of the carbon-fiber prosthetic legs he uses, is the first double amputee to have participated in a Summer Olympics and is a Paralympics champion. He's accused of premeditated murder in the Feb. 14 shooting death of his girlfriend, 29-year-old Reeva Steenkamp. Pistorius says it was a terrible accident — that he thought there was an intruder in his home and that he fired four shots through a closed toilet door after shouting at the person to leave.


text: http://techcrunch.com – There are any number of 'apps builders' out there, from Appbreeder, Buzztouch, Apps-builder, Appsbar and Mobileappwizard (you get the drift), and even Apple has patented a tool for simple apple building. However, few have received VC backing, which is why it's notable that My-Apps.com has secured $1.5 million investment from Russia's Financial Group Life, which earlier this year created a $10m tech venture fund. Prior to this My-apps attracted a $300,000 investment from Farminers seed fund and Igor Matsanyuk, one of the Mail.ru Group founders, taking funds raised to dot (IT news)


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Hurston's accomplishments are especially impressive given that she left home at 14, put herself through school at Morgan Academy in Baltimore and Howard University in Washington, D.C., working at various jobs including manicurist and maid. She arrived in New York in 1925 with $1.50 to her name.
CTnow.com, 285 Broad St., Hartford, CT 06115
A Hartford Courant website


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SXSW Rejected Lindsay Lohan's 'Canyons' Over 'Quality Issues'
A festival insider tells THR the Paul Schrader-directed thriller has "an ugliness and a deadness to it."
This story first appeared in the Feb. 1 issue of The Hollywood Reporter magazine.
Are there no film festivals willing to welcome Lindsay Lohan's The Canyons?
Sources tell THR that SXSW has joined Sundance in rejecting the erotic thriller. A festival insider tells THR that the Bret Easton Ellis-penned, Paul Schrader-directed Canyons — which features tons of nudity, including au naturel shots of Lohan — suffers from significant "quality issues."
PHOTOS: Lindsay Lohan Through the Years
Says a festival insider, "It's got an ugliness and a deadness to it." Canyons producer Braxton Pope says the filmmakers are still awaiting official word from the festival.
Never miss a story. Stay up-to-date with THR's Daily Headlines.
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