text:
Methods for growing and neutralizing or removing circoviruses, in particular porcine circoviruses, which are obtained from an infected cell culture after one or more passages in cultures of porcine, bovine or human cells are described. When the porcine circoviruses grow, a cytopathogenic effect occurs in the cell culture. The circoviruses can be neutralized by treatment with an antibody-containing substrate such as porcine serum or human immunoglobulin or be removed by a pasteurization method. Also described are a vaccine and a diagnostic aid containing inactivated or avirulent circoviruses.
1. A method for growing circoviruses, in particular porcine circoviruses (PCV), which comprises circoviruses obtained from an infected cell culture being, after one or more passages in cultures of porcine, bovine or human cells, developed in these cell cultures and a cytopathogenic effect occurring thereby.
2. A method for neutralizing or removing circoviruses from biological material, which comprises treating it with an antibody-containing substrate such as porcine serum or human immunoglobulin or subjecting it to a pasteurization method.
3. A method for detecting and quantifying antibodies directed against circoviruses by the ELISA method, which comprises circoviruses being incubated, after adsorption onto a support material, with the serum to be investigated and thus being bound to a primary antibody present in the serum, and subsequently a secondary, labeled antibody directed against the primary antibody being brought into contact therewith, and then the signal emitted by the bound, labeled antibody being measured.
4. A method for detecting and quantifying the circovirus antigen by the ELISA method, which comprises an antibody against circoviruses which is bound to a support material being incubated with the serum to be investigated for circovirus antigen, and thus the antigen being bound, and the latter being brought into contact with a labeled antibody directed against the antigen and, after the unbound, labeled antibody has been washed out, the signal emitted by the bound, labeled antibody being measured.
5. A vaccine, which comprises inactivated or avirulent circoviruses.
6. A diagnostic aid which comprises inactivated or avirulent circoviruses.
7. The use of circoviruses for investigating the capacity of a method for manufacturing pharmaceuticals of biological origin, of additives for the manufacture of pharmaceuticals or of a diagnostic aid to inactivate and/or remove circoviruses or related viruses.
[0001] The invention relates to methods for growing and quantifying the infectious or antigenic amount and determining antibodies against circoviruses, in particular porcine circoviruses (PCV). The invention also relates to methods for detecting the reduction in circoviruses and circovirus-like viruses of humans through the method for manufacturing pharmaceuticals from biological material (1).
[0002] Porcine circoviruses are isometric non-enveloped viruses about 17 nm in size and having single-stranded circular DNA of 1.76 kb. Antibodies against porcine circovirus have been found in serum from humans, mice and cattle by means of an indirect immunofluorescence detection (IFA) and by means of the ELISA method (2). Antibodies are detected in 53 to 92% of slaughter pigs (3). The clinical significance of PCV is still unknown. A new type or porcine circovirus which leads to lesions in pig tissues and can be correlated with disease symptoms has recently been detected; this so-called type 2 (PCV2) differs from the aforementioned type 1 (PCV1) mainly in terms of the pathogenicity (3 and 4).
[0003] Although porcine circovirus can be detected as contaminating agent in pig tissue cultures, it has not to date been possible to grow the virus in vitro and establish a routine test for growth and for quantitative detection of the virus, e.g. on the basis of a cytopathogenic effect (CPE), in the virus-replicating cells (2).
[0004] However, PCV is detected in vivo by means of the polymerase chain reaction in lymph nodes and cells of the spleen, tonsils, liver, heart, lungs, nasal mucosa, kidneys, pancreas and intestine (3). Since pig organs are used for human transplantation (5), porcine circovirus should be regarded as a potential risk virus for humans.
[0005] The object of the present invention was therefore to develop a method for cultivating porcine circovirus in vitro in order to be able to examine its infectivity. It was additionally intended to develop a method for neutralizing porcine circovirus by specific antibodies and removing it from biological materials from pigs, humans or other vertebrates, so that these materials can be employed without reservations directly or indirectly for therapeutic purposes, e.g. for obtaining insulin, heparin, blood and plasma, cell culture media and constituents thereof, including trypsin, and for cells for producing recombinant proteins. It was finally intended that successful growing of PCV in cell cultures would also make it possible to produce a vaccine by methods known per se. This may involve using inactivated PCT or an avirulent PCV strain (e.g. through selection of an avirulent PCV strain after adaptation to various cell cultures and/or after treatment of infected cell cultures with mutagens or after genetic modification of the PCV) as live vaccine. In addition, the antigenic material obtained from grown porcine circoviruses can also be employed for diagnostic purposes.
[0006] This object is achieved by a method for growing circoviruses, in particular porcine circoviruses (PCV), in which circoviruses obtained from an infected cell culture grow in cultures of porcine, bovine or human cells after one or more passages in the cell cultures.
[0007] For the method of the invention, porcine circoviruses obtained from a PK15 (=porcine kidney) cell culture inapparently infected with PCV were, after passage in other cell cultures, in particular porcine cell cultures, grown in these cell cultures, with a cytopathogenic effect being displayed. The presence of porcine circoviruses was in this case verified with the aid of a specific nested polymerase chain reaction (PCR). Primers with the following DNA sequences were employed for this:
[0008] 1. PCR plus: GAG AGG AAG GTT TGG AAG AGG (946-966)
[0009] 1. PCR minus: CCA CTG GCT CTT CCC ACA ACC (1358-1338)
[0010] 2. PCR plus: GGT GAA GTG GTA TTT TGG TGC C (1025-1046)
[0011] 2. PCR minus: CTA TGA CGT GTA CAG CTG TCT TCC (1326-1303)
[0012] These primers were selected from the origin of replication (7).
[0013] When carrying out the method of the invention, it was observed that the porcine circoviruses cannot be grown equally well in all cultures of various mammalian and human cells. Growth was successful in cultures of cells from various porcine organs, from bovine kidney, bovine lung and human lung. A cell culture which is very suitable for successful growth of porcine circovirus and which was developed from fetal porcine testis is deposited at the DSMZ under No. FSHO-DSM ACC2466.
[0014] The ELISA method is very suitable for quantitative detection of antibodies present in serum against porcine circovirus. This entails circoviruses being incubated, after adsorption onto a support material, with the serum to be investigated and thus being bound to a primary antibody present in the serum. A secondary labeled antibody directed against the primary antibody is then brought into contact therewith and, after the unbound secondary antibody has been washed out, the light signal emitted by the bound labeled antibody (extinction) is measured. The sandwich method known to the skilled worker is suitable for detecting the circovirus antigen, in which case an antibody against circoviruses which is bound to a support material binds the antigen in the serum to be investigated; a (labeled) antibody directed against circovirus antigen is then brought into contact therewith and, after the unbound antibody has been washed out, the light signal emitted by the bound labeled antibody (extinction) is measured.
[0015] Sera containing neutralizing antibodies against porcine circovirus are suitable for neutralizing circoviruses in biological material. Neutralizing antibodies have been found in porcine sera and human immunoglobulin (γ-globulin). A human immunoglobulin particularly suitable for neutralizing circoviruses in biological material is one obtained from high-titer human donors who have a titer of specific antibodies which is at least two to three times higher than normal donors, the average PCV antibody titer being determined on a plasma pool from at least 1000 donors.
[0016] The virus safety of pharmaceuticals of biological origin (e.g. from human blood/plasma, from cell cultures, from animal organs/tissues) is investigated as required by the authorities (e.g. CPMP/BWP/268/95: Note for guidance on virus validation studies: the design, contribution and interpretation of studies validating the inactivation and removal of viruses; CPMP/BWP/269/95 rev. 3: Note for guidance on plasma-derived medicinal products); in these so-called virus validation studies, viruses are deliberately added to material at various production steps in the method of manufacture of biologicals, and the removal and/or inactivation of the viruses by the step in the method is determined. The viruses used for this investigation either should possibly occur in the biological starting material or, if these viruses cannot be grown in vitro, are model viruses with physico-chemical properties as similar as possible to the contaminating viruses. An example of a model virus for the human circovirus TTV is PCV. In investigations of a step in the method of manufacture of therapeutic compositions from biological material—heat treatment at 60� C. in stabilized aqueous solution—it emerged that the porcine circovirus is labile and could be inactivated within a few hours; it is thus possible to demonstrate the capacity of the method of manufacturing biologicals to inactivate TTV by a heat treatment. It is possible analogously to investigate other steps in the manufacturing process for the ability to remove TTV (e.g. by precipitation, adsorption or chromatography or filtration steps) or inactivate TTV (e.g. by chaotropic salts or substances which intercalate in nucleic acids, or by irradiation with high-energy rays) using PCV. It is additionally possible to establish the capacity for inactivating and/or removing viruses also, for example, for additives in the production of pharmaceuticals, such as, for example, sera and other ingredients of media for cell cultures for producing recombinant proteins or monoclonal antibodies for affinity chromatography for purifying and concentrating active ingredients.
[0017] The invention is explained in detail by the following examples:
[0018] The culture supernatant from a PK15 culture which had been maintained for many tissue passages and which showed, five days after passaging, a positive signal for PCV in the PCR was subcultured in the ratio 1:100 on cell cultures of various cell lines which had been freshly seeded out in T25 cell culture bottles.
[0019] The following permanent cultures of porcine cells were inoculated:
[0020] Fetal porcine kidney=FPK
[0021] Fetal porcine thyroid=FPTh
[0022] Fetal porcine testis=FPTe
[0023] Fetal porcine spleen=FPSp
[0024] Fetal porcine heart=FPH
[0025] Fetal porcine skin=FPSk
[0026] Porcine kidney=PS
[0027] Ten days after inoculation, all the cultures apart from FPTh and FPSk showed cytopathogenic changes (CPE). The cell culture supernatant was harvested from the CPE-positive cultures and stored at −80� C. until used for further experiments.
[0028] A second passage of the CPE-causing agent was carried out on the homologous cell cultures by inoculating these cell cultures with the cell culture supernatant from the first passage.
[0029] A distinct CPE was evident after only four days in the PS cells; with the other cultures, the CPE was visible for the first time six days after inoculation. Using a specific PCR employing the abovementioned primers it was possible to detect PCV in the PS cell culture showing a CPE, while no PCR signal was evident in the corresponding control cells without inoculation of the PK15 cell culture supernatant.
[0030] The PCV grown in the PS cell culture was quantified in the harvested cell culture supernatant which had been centrifuged at low speed, by means of the end-point dilution method. The cell culture supernatant was diluted in 10-fold dilution steps and transferred to PS cell cultures in microtiter plates, and the PCV growth was evaluated as cytopathogenic effect (CPE). Final reading of the test took place seven days after the infection, and the virus titer (CCID50 —cell culture infective dose 50%—in log10) was calculated by methods known to the skilled worker (6). Since circoviruses are non-enveloped viruses, as a check and to confirm that the cytopathogenic effect is attributable to the growth of PCV in the cell culture, part of the PS cell culture supernatant was treated with chloroform; this method is known to the skilled worker to inactivate enveloped viruses. Comparative titration of the untreated and chloroform-treated virus suspension in PS cells revealed no difference in titer (Table 1). This result, together with the specific PCR result, confirms growth of PCV in the cell culture, in particular in porcine cells.
[0031] Starting from the virus suspension of the PS cell culture-as described in Example 2, freshly seeded cultures of various cells in T25 cell culture bottles were inoculated with a 1:100 dilution of the infectious cell culture supernatant.
[0032] In total, three adaptation passages were carried out; i.e. the PCV-infected culture supernatant was put 1:100 v/v on freshly set up cultures of the homologous cell cultures ten days after infection. The cultures with a cytopathogenic effect were tested in the PCR in order to demonstrate the identity of PCV. It surprisingly emerged from this that PCV grows not only in the porcine cell cultures shown in Example 1 but also in certain bovine and human cells, with development of a cytopathogenic effect (Table 2).
[0033] After in vitro growth of PCV in cell cultures had succeeded, the capacity of sera from various mammals to neutralize PCV was investigated. These investigations indicate in which mammals PCV grows, with subsequent seroconversion, or whether their sera contain antibodies which cross-react with PCV. However, it was not possible with the present experimental design to distinguish reliably between these two possibilities.
[0034] Various sera from individual animals (dog, cat, horse, pig, monkey) and pooled sera from several animals (cattle) or immunoglobulin concentrates from human pooled plasma were tested in the neutralization test—antibody dilution and constant amount of virus (about 100 CCID50). As Table 3 below shows, neutralizing antibodies against porcine circovirus are detectable only in porcine sera and human immunoglobulin (e.g. Beriglobin 7).
[0035] The PCV-neutralizing and non-neutralizing antibodies can also be detected with other in vitro methods known to the skilled worker, e.g. with enzyme immunoassays (EIA). Adsorption of PCV onto a solid phase (e.g. polystyrene, nylon or cellulose) with subsequent incubation of the sera to be investigated and further incubation with enzyme-labeled, secondary antibodies directed against the primary antibodies present in the sera leads to quantification of antibodies directed against PCV in the serum to be investigated.
[0036] PCV-containing cell culture supernatant from PS cells was prediluted 1:100 in 0.1 M NaOH and pipetted in a geometric dilution series into an ELISA microtiter plate (dilution buffer 0.05 M Na2CO3, pH 9.6). The color intensity was measured by methods known to the skilled worker for blocking the plate, and incubating with serum to be investigated and labeled antibodies directed against the serum to be investigated (Table 4). The example shows that antibodies against porcine circovirus are detectable only in porcine sera and human immunoglobulin concentrates.
[0037] In the manufacture of therapeutic compositions or substances intended to be employed for their manufacture from biological material it is necessary to inactivate or remove viruses which are potentially present. For this reason, the thermal stability of porcine circovirus was tested in various media in a further series of tests at 60� C. (pasteurization in aqueous solution):
[0038] a) in Eagle's minimal essential medium (EME medium)
[0039] b) in 5% strength human serum albumin solution (HSA)
[0040] c) in pasteurization buffer for blood coagulation (FVIII) products (aqueous solution stabilized with sucrose and glycine).
[0041] For this purpose, PCV was produced as in Example 2, added (spiked) 1:11 v/v to the three media mentioned above and heated in a water bath at 60� C. After the stated times, samples were taken for titration of the remaining virus and titrated on PS cells.
[0042] Final reading of the titer took place 7 days after setting up the test by observing the cytopathogenic effect under the microscope; the results are shown in Table 5.
[0043] As the results show, PCV is unstable to physico-chemical parameters such as elevated temperature. Stabilizers added to the pasteurization buffer for factor VIII products in order to stabilize this factor during the pasteurization (heat treatment at 60� C. in stabilized aqueous solution) likewise stabilize PCV to a certain extent. It is thus possible to use circoviruses for investigating the capacity of a method of manufacture or of a diagnostic aid to inactivate and/or remove circoviruses or related viruses.
[0044] Compilation of Literature
[0045] 1. Handa, A. et al., Prevalence of the newly described human circovirus, TTV, in United States blood donors. —Transfusion 40, 245-251 (2000)
[0046] 2. Tischer, I., Bode, L., Apodaca, J., Timm, H., Peters, D., Rasch, R., Pociuli, S. and Gerike, E. "Presence of antibodies reacting with Porcine Circovirus in sera of humans, mice and cattle"; Arch. Virol. 140, 1427-1439 (1995)
[0047] 3. Morovsov, I., Sirinarumitr, T., Sorden, S. D., Halbur, P. G., Morgan, M. K., Yoon, K. -I. and Paul, P. S. "Detection of a novel strain of Porcine Circovirus in pigs with postweaning multisystemic wasting syndrome" J. Clin. Microbiol. 36, 2535-2541 (1998)
[0048] 4. Hinrichs, U. et al., Erster Nachweis einer Infektion mit dem porzinen Circovirus Typ 2 in Deutschland. —Tier�rztliche Umschau 54, 255-258 (1999)
[0049] 5. Allan, J. S. "Nonhuman primates as organ donors?" Bull. WHO 77 (1), 62-63 (1999)
[0050] 6. K�rber, C. "Beitrag zur kollektiven Behandlung pharmakologischer Reihenversuche" Arch. Exp. Path. Pharmak. 162:480-487 (1931)
[0051] 7. Mankertz, A. et al., 1997. Mapping and characterization of the origin of DNA replication of porcine circovirus. J. Gen. Virol. 71:2562-2566.
Referenced by
Legal Events
Original Image


text:
If nothing else, Three Messages and a Warning proves that anthology editors hold far more power than the individual authors. The problem is not so much that Three Messages fails to offer any excellent Mexican "stories of the fantastic," but that those tales are few and poorly placed within the book as a whole. For example, a number of above-average stories are clustered toward the end of the book, so that anyone prone to reading anthologies chronologically will be tempted to give up reading before they find gold.
If anything, it just seems like the people editing Three Messages forgot to pay attention—how else would a poem (and a mediocre poem at that) find its way into a book of short stories? How else would so many mediocre stories make the cut? Overall, the thirty-four "stories" in Three Messages provide a study in quantity over quality, a survey of Mexican literature that does little credit to Mexican authors. However, whether by purpose or chance, there are some diamonds in the rough, tales with original voices and surprising endings, the kind of stories you find yourself telling your friends about later. Rather than leaving you to sort through the entire collection (or skip it entirely) I'll offer you what, in my opinion, are the highlights. The stories sort themselves into three categories:
Category One: The Very Good.
1. "The President without Organs" by Pepe Rojo.
In retrospect, this story captures exactly what I was hoping to find in Three Messages: an imaginative subject explored by an expert storyteller. The story unfolds through a series of press releases detailing the various surgeries the President undergoes in order to cure his increasingly bizarre illnesses, as well as mini-narratives about citizens reacting to the news. Witty and controversial, the story is a hilarious parody of the roles of citizens, government officials and the media in religious and political systems. Then again, I'm bound to love any story that contains a section that reads only, "NATIONAL TIME-OUT DAY."
2. "Photophobia," by Mauricio Monteil Figueiras.
You can tell from the start that "Photophobia" is more sophisticated than most stories in this collection—the vocabulary is complex, the concept unquestionably cerebral. An apocalyptic narrative is told through stream-of-consciousness storytelling that cleverly distracts from the story's premise until the ending begins to shed some light on the narrator's purpose and motives. The tale stands out in this populist collection of stories like a sore thumb, but I'm glad it was included. Here is a typical (and excellent) sentence:
Eternity, he thought, pocket apocalypses: man has not learned the lessons of history, he is still the ignorant student who recorded his confusion in the caves of Altamira—it's just that the caves have become tabloids.
3. "Nereid Future," by Gabriela Damián Miravete.
Imagine a modern, Mexican version of Margaret Oliphant's short story from 1869, The Library Window and you'll arrive at "Nereid Future." The story, told in the second person, is about a girl who falls in love with a long-dead author through his books. The narrative gets increasingly meta as the girl begins to believe that the author loves her back. Intertextuality and female identity earn the spotlight in this short story, which contains one of those perfect endings where you should have seen it coming from the start, but still catches you by surprise.
4. "The Drop" by Claudia Guillén.
In "The Drop," a depressed young woman refuses to leave her room, watching drops of water fall to the floor. Her mother (the stated villain of the piece) claims that if the dripping stops, her child will die. A visiting doctor learns about himself as he studies the girl. That's it, the entire premise. But the story is well-told, the ending surprising, and it's the kind of eerie tale that sticks with you.
5. "Variations on a Theme by Coleridge," by Alberto Chimal.
Three Messages includes plenty of short-short stories; this is my favorite example, a page-and-a-half-long gem. It begins, "I got a call. It was me, calling from a phone I lost the year before. I asked myself where I had found the phone. I answered myself that it was in such and such cafeteria that I couldn't remember anymore." The story gets increasingly meta and hilarious, drawing its premise from the capabilities of modern technology, its humor from repetition and its pathos from the ways we judge ourselves.
Other favorites: "Lions" by Bernardo Fernández, "Wittigenstein's Umbrella" by Óscar de la Borbolla, "Mr. Strogoff" by Guillermo Samperio.
Category Two: The Mediocre.
Most of the book falls into this category: stories that build up but go nowhere ("The Guest"); stories that you swear you've read before ("Three Messages and a Warning in the Same E-mail"); stories with one original gimmick, a clever premise or punch line that amuses without earning long-term appreciation ("A Pile of Bland Desserts", "Wolves"); even a few pieces that don't fully cross the cultural divide ("The Nahual Offering").
These are not awful stories. I enjoyed reading some of them. But when I forget they existed in a week or two, I won't feel the loss.
Category Three: The Ugly.
In my opinion the worst of the collection (besides that very random poem, "Mannequin") are the stories that are unbearably trite, the stories that fit a shallow American understanding of Mexican culture to a T. I'm speaking mostly of the first story in the collection, "Today, You Walk Along a Narrow Path," a tale about Día de los Muertos with the most predictable "surprise" ending in the entire book. There are others that fit this category, of course . . . but the line between "mediocre" and "ugly" seems awfully thin in my mind, so I think I'll let future readers sort out those stories on their own.
Founded in 1960 by such creative pioneers as George Perec, Raymond Queneau and Italo Calvino, the Oulipo, shorthand for Ouvroir de littérature potentielle, came about in when a group of writers and mathematicians sought constraints to find new structures and. . .
There's little to say about a series of prose poems that willfully refuse to identify pronoun antecedents. Or perhaps there are a million things. The poems in Morse, My Deaf Friend— the chapbook by Miloš Djurdjević published by Ugly Duckling. . .
The Crimson Thread of Abandon is the first collection of short fiction available in English by the prolific Japanese writer and all-around avant-garde trickster Terayama Shūji, who died in 1983 at the age of 47. This collection would be important. . .
Last year, NYRB Classics introduced English-language readers to Catalan writer Josep Pla with Peter Bush's translation of The Gray Notebook. In that book, Pla wrote about life in Spain during an influenza outbreak soon after World War I, when. . .
"Your bile is stagnant, you see sorrow in everything, you are drenched in melancholy," my friend the doctor said.
bq. "Isn't melancholy something from previous centuries? Isn't some vaccine against it yet, hasn't medicine taken care of it yet?" I. . .
What to make of Vano and Niko, the English translation of Erlom Akhvlediani's work of the same name, as well as the two other short books that comprise a sort of trilogy? Quick searches will inform the curious reader that. . .
The opening of Jón Gnarr's novel/memoir The Indian is a playful bit of extravagant ego, telling the traditional story of creation, where the "Let there be light!" moment is also the moment of his birth on January 2nd, 1967. Then. . .

subset: commoncrawl


text:
Kawaii! Brother launches special edition Hello Kitty electronic labeler
The red and white Hello Kitty-themed labeler comes with 97 frames, of which 22 are Hello Kitty ones. Together with 34 Hello Kitty symbols and Hello Kitty laminated tape, it's a must-have for Hello Kitty lovers.
Shootout: From 0 to 100, the fastest charging phones available
With smartphone resolutions getting higher and higher, battery life has struggled to keep up. As a result, reducing re-charge times has become the next best thing. But which fast charging technology is the best? And what phone can you find it on? We find out.
Rumor: Patent for an EF/EF-S adaptor hints to a Canon full-frame mirrorless camera
A patent showing an EF/EF-S adaptor for a non-existent full-frame Canon camera has appeared online and it seems to be causing a bit of a stir online. Is this a possible full-frame mirrorless body from Canon?
First batch of Steam Machines pre-orders are sold out
Valve's plan to dominate the living room is off to a great start as the company reports the first batch of Steam Machines pre-orders are completely filled.
Venus Optics has just produced the World's widest macro lens, the Laowa 15mm f/4
Venus Optic's new Laowa 15mm f/4 is full-frame wide-angle macro lens that has an ultrawide 15mm field of view and 1:1 Macro ability.
Facebook is watching you more closely than you think
Facebook is rolling out more detailed tracking of how you watch videos in your newsfeed, including whether you increase the volume or watch the video in full screen. This is part of its bid to provide more relevant content for users that goes beyond traditional engagement statistics.
Samsung to launch more Tizen-based smartphones this year?
Google and Samsung have always had a somewhat tenuous relationship and the latest rumor from a Reuters source may signal Samsung's desire for more independence and less reliance on Google's platform.
EKWB, XSPC to launch AMD Radeon R9 Fury X water block
EKWB and XSPC have announced the impending availability of full-cover GPU water blocks for AMD's flagship Radeon R9 Fury X.
AppleCare for Mac will now cover all batteries that hold less than 80% charge
Apple has just revised the terms of its AppleCare protection plans for Mac to cover all batteries that retain less than 80% charge.
OnePlus 2 to have a fingerprint sensor that unlocks "faster than Touch ID"
OnePlus has confirmed the third official spec of its upcoming flagship smartphone - the OnePlus 2 will sport a fingerprint sensor that works "faster than Touch ID".
This strap will add 30 hours of additional battery life to your Apple Watch
Called the Reserve Strap, it has built-in lithium-polymer cells that charge the Apple Watch through the watch's hidden accessory port.
Somebody pointed a Nikon Coolpix P900 with 83x optical zoom at the moon (video)
Here's a video to lessen your Monday blues. YouTuber Naturbeobachtungen von Lothar Lenz pointed a Nikon Coolpix P900 with its 83x optical zoom at the moon on a clear day and got this amazing video out of it. Just watch it for yourself.
Intel Compute Stick: Jack of all trades but master of none
The Intel Compute Stick is a versatile pocket PC, but this versatility does come at a price. The Compute Stick does many things, but none of them quite well enough.
Disney bans selfie sticks
The happiest place on Earth deems selfie sticks a threat to safety and is banning them from its premises.
Facebook testing Snapchat-inspired photo uploader that includes swipe-able filters and text
Facebook is testing a new photo uploader that includes Snapchat-esque features like swipe-to-preview filters, stickers, and text overlays. The new feature is currently only available to select iOS users.
New Private Chat feature on BlackBerry's BBM removes names from conversations
BlackBerry has updated BBM with a new feature called Private Chat, which allows users to hold anonymous conversations by removing names from chat windows.
iOS 8.4 to be released 11pm on Tuesday, an hour ahead of Beats 1 launch
Apple's latest mobile OS, iOS 8.4, will be available from 11pm tomorrow, an hour ahead of the launch of Apple's radio service Beats 1.
Hands-on with the new Canon PowerShot G3 X and limited edition SG50 PowerShot G7 X
We scored some time with a pre-production Canon PowerShot G3 X and the limited edition SG50 PowerShot G7 X.
Bloomberg: Early production of new Apple iPhones with Force Touch has begun
Bloomberg reports that Apple has started early production of new iPhone models with Force Touch. The upcoming iPhones will likely sport the same design as the current iPhone 6 models and manufacturing is expected to ramp up next month.
Apple iOS 9's Low Power mode enables longer battery life by processor throttling
Apple iOS 9's Low Power mode is able to extend battery life on your iOS device by throttling the processor. By what extent is performance affected? Read on to find out more.


text:
China Bird Flu Death Toll Rises to Six
April 5 (Bloomberg) -- Sara Eisen reports on today's top news headlines on Bloomberg Television's "Bloomberg Surveillance." (Source: Bloomberg)
Most Recent Videos
Malaysia PM Caught Up in 1MDB Money Trail Probe
53:10 - Malaysia's prime minister has been forced to deny being a thief after reports of up to $700M of public money ended up in his bank account. A task force has now raided the offices of three Malaysian companies linked to 1MDB, the debt-ridden state investment firm at the center of the scandal. Bloomberg's Haslinda Amin reports on "First Up." (Source: Bloomberg)


text:
Kayak Videos
What We Came For - First Descent: Michoacan
The group is eager to get back to paddling after a couple of days without good water flows. The Rio...
by pete@extreme
Tuesday Apr 01, 2014
613 Views
Scouting Uncharted Waters - First Descent: Michoacan
by pete@extreme
Tuesday Apr 01, 2014
265 Views
The Journey Begins - First Descent: Michoacan
Six adventure kayakers and friends arrive in Mexico for a three-week trip to explore uncharted rivers...
by pete@extreme
Tuesday Apr 01, 2014
217 Views
GoPro: Ken Block's Gymkhana 6 GoPro Edition
GoPro presents Ken Block's 6th installment of his world famous Gymkhana series. This time, Ken looks...
by Andy
Friday Jan 31, 2014
7.299 Views
GoPro - Hero3 Black Edition VS ISAW - Extreme A3. We strapped on our wakeboards and helmet cams and...
by sam@extreme
Monday Nov 04, 2013
13.060 Views
GoPro: HERO3+ Black Edition: Smaller, lighter, mightier still.
Shot 100% on the new HERO3
by Andy
Monday Oct 07, 2013
3.612 Views
adidas sickline Kayak world championships 2013
by soulelec
Wednesday Sep 25, 2013
10.801 Views
GoPro: Desert Lines with Ronnie Renner and Davi Millsaps
Shot 100% on the HERO3® camera from GoPro.com. Ronnie Renner and Davi Millsaps carve up the desert...
by sam@extreme
Thursday Jun 06, 2013


text:
Studying for the MCSE Win2000
Continue Reading This Article
Enjoy this article as well as all of our content, including E-Guides, news, tips and more.
By submitting your personal information, you agree to receive emails regarding relevant products and special offers from TechTarget and its partners. You also agree that your personal information may be transferred and processed in the United States, and that you have read and agree to the Terms of Use and the Privacy Policy.
If I read your message correctly, you've taken 70-210 and 70-215 and plan to finish the two remaining core exams: 70-216 and 70-217. After that, you plan to take 70-219, 70-081 and 70-222. I am happy to say that this will indeed qualify you for the Windows 2000 MCSE and that you are not taking any unnecessary exams in this planned sequence. Go ahead and follow your plans, and you should have an MCSE waiting for you at the end of that road.
Dig Deeper
Find more PRO+ content and other member only offers, here.
Related Q&A from Ed Tittel, Networking Career, Certification Expert
How to fix shortage of cyber security professionals?
The cyber security skills shortage comes from an aging workforce and lack of interest in security among students. Emphasis must be put on education ...continue reading
How are VMware certifications valued right now?
Career expert Ed Tittel explains how the value of VMware certs has changed since 2005.continue reading
What is the proper certification path for becoming a network administrator?
Career expert Ed Tittel helps guide a reader toward the correct certs for meeting his goal of working in the network administration field.continue reading
Have a question for an expert?
Please add a title for your question
Get answers from a TechTarget expert on whatever's puzzling you.
Meet all of our Windows Server experts
View all Windows Server questions and answers


text:
ST. PAUL, Minnesota — The Minnesota Wild will host Stanley Cup champion Chicago three times this season, including their outdoor game Feb. 21.
The NHL schedule was unveiled Thursday.
The Wild start the regular season at Colorado Oct. 8 before facing St. Louis in their Saturday night home opener Oct. 10. Dallas and Winnipeg are the other rivals the Wild will host three times, among their 29 games against the Central Division. The Blackhawks, who ousted the Wild from the playoffs in each of the last three years, will visit Minnesota Oct. 30 and March 29. The game Feb. 21 will be at TCF Bank Stadium at the University of Minnesota.
The Wild also play six of their last nine games at home, wrapping up the regular season against Calgary April 9.


text:
The Style Gallery is a great new way for you to view, love, and share outfit photos. It's meant to showcase
all the expressive, creative, and inspirational personalities of our community!
Love you too!
Your email has been sent!
If you've been searching for a charming new frock, then you're in luck! The A-line silhouette and snazzy cobalt-blue hue of this dress flaunt effortless femininity, while the golden buckle of its glossy black belt exudes sophistication. Wearing this pocketed, stretch-cotton piece, which is produced in the UK by Closet, is always a fashionable chance worth taking!
All measurements in inches.
Let us know if you need it! We reorder based on community feedback and demand, so your voice is very important. We'll email you if we can bring it back.
Remarkable fashion & decor, inspired by our community, & delivered with uncommon care.
Copyright ©2002-2015 ModCloth.com. All Rights Reserved.
Site Map
CA Notice
Privacy & Security
Terms of Use


text:
BEDOUIN SOUNDCLASH: Light The Horizon (Pirates Blend)
Lighter in tone than the trio's last LP, Light The Horizon paints a good picture of a band that uses reggae as a jumping off point for a broader pop hybrid. The new album might not be as "full on" … Continue reading
The Beast goes on
The Kelowna-filmed indie comedy The Beast of Bottomless Lake gets its first Vancovuer screening in October. The movie screens Saturday Oct. 2 at 1 p.m., with actors and behind the scenes talent on hand for a post-screening Q and A. … Continue reading
BLONDE REDHEAD: Penny Sparkle (4AD)
Once again pairing up with Depeche Mode producer Alan Moulder for mixing duty, the trio further expands its sonic adventures into more taut, dance-oriented pop rather than the off-kilter indie rock experimentalism of past efforts. Far from robbing the group … Continue reading
UNDERWORLD: Barking (Cooking Vinyl)
At about 90 seconds into "Bird 1," the head is bobbing, the pulse is starting to keep pace with the bubbling bassline and you know it's an Underworld joint. The pioneering UK electronic combo of Karl Hyde and Rick Smith … Continue reading
MICHAEL FRANTI & SPEARHEAD: The Sound of Sunshine (EMI)
Did Franti become more commercial or did the world suddenly catch up to his incredibly potent message of music, love & politics? It's a question that was first posed when "Say Hey (I Love You)" suddenly became a multi-platinum hit … Continue reading
THE BLACK ANGELS: Phosphene Dream (Blue Horizon)
This Austin psychedelic drone crew has been sitting on its third album due to label issues, specifically not having one to release it after being dropped from its major signing before anything came out. Now that Seymour Stein and Richard … Continue reading
MI-4 fuse to be lit in Vancouver
The producers of the Tom Cruise action franchise Mission Impossible 4 have already started setting up shop in Vancouver, one of multiple locations for the globe-trotting spy thriller. Dubai and Prague are the others. Director Brad Bird (The Incredibles… Continue reading
SUNS OF ARQA: Know Thyself? (Interchill)
Over 35 albums spanning a time period that goes back beyond the whole concept of world-fusion electronic music, this project helmed by producer Michael Wadada has consistently released intricate, delicate, Indian classical music-oriented platters. It's latest four song session doesn't … Continue reading
REVIEW: Score: A Hockey Musical
Review by Jay Stone, Postmedia News: A bold idea — A musical about hockey! Where everyone sings their lines! And the players dance on ice! — too frequently just lies there on the screen. Director Michael McGowan has a talented … Continue reading
REVIEW: Uncle Boonmee Who Can Recall His Past Lives
Review by Jay Stone, Postmedia Network: This dreamlike Thai film moves beyond logic into some fairy tale enchantment: it will delight some viewers and frustrate others. It's about a dying man who sees ghosts — relatives, a monkey man — … Continue reading


text:
Latest News
Hoyleton Golf Classic
Download the Golf registration here: http://www.hoyleton.org/wp-content/…/2015/05/Golf-Invite.pdf When: Friday July, 24th Where: Tamarack Golf Course, 800 Tamarack Ln. • Shiloh, Illinois 62269 Schedule: 11:00 am Registration and Lunch, 12:00 Shotgun Start. Dinner & awards following the game. Format: 4 person scramble, 3 flights, 1st, 2nd & 3rd place awards. For more information contact: Darla at 618-493-7575 ext. 1335
Hoyleton Ministries seeks to enable all people, young and old alike, to realize the wholeness of life that God intends. This will be accomplished with the compassion of Jesus Christ and the power of the Holy Spirit reaching out to meet the physical, emotional, intellectual, spiritual, and social needs of those with whom we journey.
The mission of Puentes de Esperanza is to transform the lives of Latino children and families in southern Illinois through community based ministries directed at their physical, emotional, social and spiritual needs.
Our Values
Fairness and Honesty
Faithful and Faith-Based Committed to Program Growth Committed to Families Excellence


text:
The House of the SpiritsPaperback
List price $14.00
You save $2.75 19% off
Free delivery worldwide
Dispatched in 2 business days
When will my order arrive?
- Publisher: Black Swan
- Format: Paperback | 496 pages
- Dimensions: 126mm x 194mm x 28mm | 322g
- Publication date: 3 February 1994
- Publication City/Country: London
- Sales rank: 6,689
Product description
Spanning four generations, Isabel Allende's magnificent family saga is populated by a memorable, often eccentric cast of characters. Together, men and women, spirits, the forces of nature, and of history, converge in an unforgettable, wholly absorbing and brilliantly realised novel that is as richly entertaining as it is a masterpiece of modern literature.
Add item to wishlist
Other people who viewed this bought:
The Great Gatsby
The Unbearable Lightness of Bein...
USD$7.94 - Save $2.95 27% off - RRP $10.89
The Perks of Being a Wallflower
USD$8.40 - Save $4.05 32% off - RRP $12.45
When God Was a Rabbit
USD$9.83 - Save $4.17 29% off - RRP $14.00
The Elegance of the Hedgehog
USD$10.08 - Save $3.92 27% off - RRP $14.00
The House of the Spirits
USD$12.01 - Save $3.55 22% off - RRP $15.56
Other books in this category
The Great Gatsby
The Book Thief
USD$11.07 - Save $1.38 11% off - RRP $12.45
The Husband's Secret
USD$9.88 - Save $2.57 20% off - RRP $12.45
We are All Completely Beside Our...
USD$9.08 - Save $1.81 16% off - RRP $10.89
The Elegance of the Hedgehog
USD$10.08 - Save $3.92 27% off - RRP $14.00
The Perks of Being a Wallflower
USD$8.40 - Save $4.05 32% off - RRP $12.45
- Categories:
Author information
Isabel Allende was born in Lima, Peru. She has recently lived in Caracas, Venezuela, with her husband and two children. Her first two novels, The House of the Spirits and Of Love and Shadows are published by Black Swan. The House of the Spirits was made into a film starring Meryl Streep, Glenn Close, Jeremy Irons, Winona Ryder, Vanessa Redgrave, Antonio Banderas and Keanu Reeves.
Review quote
"'This is a novel like the novels no one seems to write anymore: thick with plot and bristling with characters who play out their lives over three generations of conflict and reconciliation. A novel to be read for its brilliant craftsmanship and its narrative of inescapable power'" El Pais, Madrid "'Intensely moving. Both entertaining and deeply serious'" Evening Standard "'Announcing a truly great read: a novel thick and thrilling, full of fantasy, terror and wit, elaborately crafted yet serious and accurate in its historical and social observations'" Die Welt, Berlin "'A remarkable achievement...a big book that can comprehend the history of a nation, and so many lives, with love'" The Times
Editorial reviews
A strong, absorbing Chilean family chronicle, plushly upholstered - with mystical undercurrents (psychic phenomena) and a measure of leftward political commitment. (The author is a cousin of ex-Pres. Salvador Allende, an ill-fated socialist.) The Truebas are estate-owners of independent wealth, of whom only one - the eventual patriarch, Esteban - fully plays his class role. Headstrong and conservative, Esteban is a piggish youth, mistreating his peons and casually raping his girl servants . . . until he falls under the spell of young Clara DelValle: mute for nine years after witnessing the gruesome autopsy of her equally delicate sister, Clara is capable of telekinesis and soothsaying; she's a pure creature of the upper realms who has somehow dropped into crude daily life. So, with opposites attracting, the marriage of Esteban and Clara is inevitable - as is the succession of Clara-influenced children and grandchildren. Daughter Blanca ignores Class barriers to fall in love with - and bear a child by - the foreman's son, who will later become a famous leftwing troubadour (on the model of Victor Jara). Twin boys Jaime and Nicholas head off in different directions - one growing up to become a committed physician, the other a mystic/entrepreneur. And Alba, the last clairvoyant female of the lineage, will end the novel in a concentration camp of the Pinochet regime. Allende handles the theosophical elements here matter-of-factly: the paranormal powers of the Trueba women have to be taken more or less on faith. (Veteran readers of Latin American fiction have come to expect mysticism as part of the territory.) And the political sweep sometimes seems excessively insistent or obtrusive: even old Esteban recants from his reactionary ways at the end, when they seem to destroy his family. ("Thus the months went by, and it became clear to everyone, even Senator Trueba, that the military had seized power to keep it for themselves and not hand the country over to the politicians of the right who made the coup possible.") But there's a comfortable, appealing professionalism to Allende's narration, slowly turning the years through the Truebas' passions and secrets and fidelities. She doesn't rush; the characters are clear and sharp; there's style here but nothing self-conscious or pretentious. So, even if this saga isn't really much deeper than the Belva Plain variety, it's uncommonly satisfying - with sturdy, old-fashioned storytelling and a fine array of exotic, historical shadings. (Kirkus Reviews)


text:
jasonphoto... Photographer
Lilya MaModel Female Tartu, Tartu, Estonia
Mayhem # 3069003
About MeI am no professional model. On this site I would like to try myself at modelling. I am fine with both professional or beginner photographers, if their intentions are serious. Please write to me on this site and we can discuss further.
jasonphoto... Photographer
Alexander Korobov Photographer
Giorgio Magistrelli Photographer
Stefano Pi Photographer
eleonoradi... Photographer
See Me Now Pictures Photographer
Loading Content


text:
Can this ring be sized down by a half size? Is it possible to alter this particular ring if it is a little big?
I love this ring. Bought it for myself along with another smaller (2 TCW) eternity band to wear as my wedding bad. The 2 ct ring is nice but not quite as well made, the stones are a little varied in size and setting if you look closely, but THIS RING is amazing. The setting is perfect, the stones are uniform, I checked them with a 10x jewelers loupe and they are as described. It is very sparkly ring and complements my right hand well. It is just as beautiful and sparkly as my engagement ring and wedding bad. Thing ring will appraise for much more..... you will NOT be disappointed. Since the band is narrow, you can probably get a way with half a size smaller than you would order in a band that is wide, so keep that in mind as eternity rings are not able to be sized. I was pleasantly surprised by this ring, and Overstock stands behind their product, has excellent customer service no matter what the issue. BUY WITH CONFIDENCE....
Make an unquestionable statement of love with this stunning 5 carat diamond eternity band. Gleaming with 15 to 17 prong-set diamonds, this brilliant band is perfect as a promise ring, a wedding ring, or an anniversary gift. Gift box included.
ITEM#: 10715940This gorgeous eternity band features 5 carats of glittering diamonds. The white gold setting sets off the fiery diamonds and adds extra sparkle to this exquisite ring. With a G-H color rating and an I1-I2 clarity rating, these diamonds are truly remarkable. This ring make an amazing wedding band, anniversary band or special piece just to say "I love you."
- Classic engagement ring sparkles with an icy diamond
- Diamond engagement ring is beautifully accented with rosy pink sapphires
- Jewelry is crafted of lustrous 14-karat white gold
- One round prong-set diamond
- Diamond color is H-I, clarity is I2-I3
- Total diamond weight is 1/4* carat
- Two round bezel-set pink sapphires measure 1.75 mm
- Total gem weight is .06 carat
All carat weights and measurements are approximate and may vary slightly from the listed dimensions. *T.W. (total weight) is approximate. 1/4 carat T.W. may be .22 to .28 carat. Treatment code N (diamond) and E (pink sapphire). See Treatment Guide for further information.
Click here for Ring Sizing Chart
This item is part of the Miadora Wedding Collection
|Diamond Color
|G-H White
|Total Diamond Weight
|Over 3 Carats
|Ring Style
|Eternity Prong
|Diamond Cut
|Gold Karat
|Gemstone Color
|Band Width
|4-5 mm
|Birthstone Month
|Gold Red White White G-H
|Jewelry Setting
|Metal Color
|Product Features
|Certified Diamond
|Setting: 4 mm W
|18k white gold, diamonds
|Model Number
Customer Reviews
- Disclosures & Guidelines
- Filter By:
- Most Helpful
- Most Recent
I love this ring. Bought it for myself along with another smaller (2 TCW) eternity band to wear as my wedding bad. The 2 ct ring is nice but not quite as well made, the stones are a little varied in size and setting if you look closely, but THIS RING is amazing. The setting is perfect, the stones are uniform, I checked them with a 10x jewelers loupe and they are as described. It is very sparkly ring and complements my right hand well. It is just as beautiful and sparkly as my engagement ring and wedding bad. Thing ring will appraise for much more..... you will NOT be disappointed. Since the band is narrow, you can probably get a way with half a size smaller than you would order in a band that is wide, so keep that in mind as eternity rings are not able to be sized. I was pleasantly surprised by this ring, and Overstock stands behind their product, has excellent customer service no matter what the issue. BUY WITH CONFIDENCE.Read More
Beautiful Ring...
The ring is beautiful and truly sparkles. I have looked around and for the price, the quality is very good. I am very pleased with my purchase and look a chance with the online purchase. Glad that I did. I was only disappointed that the ring did not come with a certificate of authenticity.Read More
Very Disappointed with Purchase
This ring looks stunning online and in the photo, and the quality is supposed to be very good by reviewing the online info from Overstock. The ring was dark, and when looking through a magnifying glass, you can clearly see the discoloration and scratches all over the entire ring. Very disappointed, so I returned it. Unfortunately, I had to continue contacting them for the credit as well.Read More
Exceeded Expectation
I purchased this ring after several weeks of reviewing this listing and comparing it to other bands I found from other jewelers. I finally ordered it last week and I couldn't be happier with it! The ring does sparkle and you really have to hunt for the inclusions that earn it the I1-I2 rating. To be honest, I can't find any inclusions without magnification. The color is great for the H-I rating. Definitely tends toward H rather than I. It's sparkly, sizeable, comfortable and a great deal. This is my second Overstock jewelry purchase and must expectations are constantly exceeded.Read More
this ring is gorgeous!
I had been looking for an eternity band for about a year and I when I found this ring, I couldn't believe the price. I had it appraised right after i got it and it appraised for ****. It is absolutely stunning and I couldn't be happier!Read More
Beautiful ring
This ring is a great value and a super deal. Having worked in the jewelry industry for a little while, I was skeptical of the quality claims for this ring. I must say I was surprised when I received the ring and had to study the ring very closely with the naked eye to identify the flaws that earn this ring the "I1-I2" rating. Not to say you can't find the inclusions, but for the money paid you are truly getting a great ring. I didn't give this 5 stars because I believe the value was great, but not a steal. Buy this ring for your wife or... ladies buy it for yourself. You will not be disappointed!Read More
diamond eternity band
This is the most etraordinary ring. I had been wanting for an eternity band for years , when my husband just showed up with this one. The diamonds are to die for.They are top quality and have the most amazing sparkle and color. I dont ever want to take the ring off my finger...Read More
Gorgeous Ring!
I have been looking for an eternity band for a long time. THe ones I had found were either too expensive or the diamonds too small. Although this was a very pricey purchase for me, it was well worth the money!! The diamonds truly sparkle and the size of the diamonds are as shown in the picture, very substantial. My engagement ring has a beautiful clear diamond, I was afraid this ring might not match in clarity and color but it really does! Buying such a substantial jewelry purchase online also really concerned me. The quality of any of the diamonds (earrings, bracelet, necklace) I had previously bought from Overstock was always wonderful so I took the chance. Needless to say, this ring will NOT be returned. I love it. For the price you are paying, this ring is well worth the investment.Read More
Excellent purchase
After having lost my eternity ring last year, I decided it was time to replace it and since I was going on a trip to St. Thomas, V.I. (where they say everyone should buy jewelry), I decided to look there first. I was so disappointed in the quality of diamonds there and insecure about a purchase that I went back to my suite and got on-line with Overstock. I immediately found the ring I was looking for and ordered it. It was delivered incredibly fast and it's absolutely outstanding. The diamonds sparkle beautifully and I certainly cannot see any inclusions in the diamonds and I doubt anyone will be taking out a magnifier to find them. For the price, this ring is an incedible purchase. It feels true to size on my finger and comfortable to wear. What a terrific ring.Read More
Questions & Answers
Hello ring question, unfortunately this ring cannot be sized due to the design. Please let us know if you have additional questions. Thanks for shopping with us.
Can this be returned?
Hello Ngozi, you will be contacted by Customer Care.
Shopping Tips & Inspiration
Wedding Party Gift Packs
Make time to remember the people who will help you celebrate your big day with personalized gifts for the bridesmaids and groomsmen.
Shopping for the Modern Bride - Wedding Bands
Shopping for the Modern Bride: Wedding Bands from Overstock.com. Our guides provide customers with information about shopping for modern wedding bands and advice about our many brand-name products.
Tips on Buying Women's Wedding Bands
Tips on Buying Women's Wedding Bands from Overstock.com. Our guides provide customers with tips on buying women's wedding bands and advice about our many brand-name products.
Best Reasons to Shop Women's Tungsten Wedding Bands
Best Reasons to Shop Women's Tungsten Wedding Bands from Overstock.com. Our guides provide customers with the best reasons to shop women's tungsten wedding bands and advice about our many brand-name products.
Diamond Cuts Fact Sheet
Diamond Cuts Fact Sheet from Overstock.com. A diamond's cut is crucial in displaying the stone's quality. Here's what you need to know when you're shopping for diamonds.
Diamond Buying Guide
Diamond Buying Guide from Overstock.com. Diamond jewelry is an investment that can last for generations, so it's important to know what to look for before you shop.
How to Buy Colored Diamonds
How to Buy Colored Diamonds from Overstock.com. Choosing and purchasing colored diamonds is a very different process than selecting colorless or white diamonds.
How to Buy a Certified Diamond
How to Buy a Certified Diamond from Overstock.com. The best way to ensure that the diamond jewelry you are buying is a good investment is to check that the diamonds are certified.
FAQs about Black Diamond Jewelry
FAQs about Black Diamond Jewelry from Overstock.com. Here are the answers to the most frequently asked questions about black diamond jewelry. Learn about black diamonds before you shop!
Black Diamond Buying Guide
Black Diamond Buying Guide from Overstock.com. Black diamonds have become an increasingly popular design element in diamond jewelry. Learn more about black diamonds.
How to Care for Clarity Enhanced Diamonds
How to Care for Clarity Enhanced Diamonds from Overstock.com. Clarity enhanced diamonds don't need a lot of extra care, just some routine maintenance.
Shipping & Returns
Usually leaves our warehouse in 1-2 business days. *
Standard Return Policy for Jewelry:
Items must be returned in new or unused condition and contain all original materials included with the shipment.
More Details
Shipping Information:
Our standard shipping method is ground shipping. We also offer expedited shipping for most items. Your final
shipping cost and estimated delivery timeframe will be available at checkout.
Standard shipping details
Expedited shipping details
* For your protection, all orders are screened for security purposes. If your order is selected for review, our Loss Prevention Team may contact you by phone or email. There may be a two business day delay to process your order.
** Most Oversize orders are delivered within 1-4 weeks. Some orders may take 6 weeks to be delivered.


text:
Please note that the GMO Compass Database currently is being expanded and updated. Please check back for new entries.
The original application also included cultivation in the EU, but this
part was withdrawn.
EFSA: Scientific opinion
Commission Decision
European Union Reference Laboratory for GM Food and Feed
© 2015 by GMO Compass. All rights reserved. | Imprint | website created by webmotive


text:
IMPORTANT: Please click "End Session" to clear your personal information. (Credit Card info is always hidden from view).End Session
Cookies are not enabled in your browser.
You must have Cookies enabled for the best experience on our site and to make a purchase.
Learn how to enable Cookies in your browser or call 1-800-426-8020 for assistance.
7 items
Women's Essential Slub 3/4-Sleeve Pullover Shirt - Stripe$35.00-$40.00 $19.99
Women's Favorite Funnel-Neck Shirt - Stripe$45.00-$50.00 $17.99
Women's Favorite Long-Sleeve V-Neck T-Shirt$25.00-$28.00 $14.99
Women's Pima Scoop-Neck T-Shirt - Solid$35.00-$40.00 $14.99
Women's Pima Striped Scoop-Neck T-Shirt$40.00-$45.00 $14.99
Women's Gate Check Henley T-Shirt$40.00-$45.00 $24.99


text:
Thanks for redeeming.{{else}}
Success! Thanks for repurchasing.{{/if}} {{else}} {{#if Expired}}
Increase my visibility!{{else}} {{#if IsMultiPack}}
{{#if HasMultiPack}} You have {{MultiPackCount}} top spot(s) left. Now loading you into the search listings... {{else}} You have used all top spots. Now loading you into the search listings... {{/if}}{{else}}
We are loading you into the search listing pages...{{/if}} {{/if}} {{/if}} {{/if}}
{{Current}} just saw you!{{/if}} {{#if LastTopSpotPurchaseIsMulti }} {{#if HasAnyTopSpot}}
{{#if HasFreeTopSpot }} {{ UnclaimedTopSpotFree }} Free {{/if}} {{#if HasPaidTopSpot }} {{#if HasFreeTopSpot }} & {{/if}} {{ MultiPackCount }} {{/if}} Top Spot{{#if Plural}}s{{/if}} Left{{else}}
0 Top Spot{{#if Plural}}s{{/if}} Left{{/if}} {{else}} {{#if HasFreeTopSpot }}
{{ UnclaimedTopSpotFree }} Top Spot{{#if Plural}}s{{/if}} Left{{/if}} {{/if}} {{else}} {{#if HasResults}}
{{Current}} see you now!{{else}} {{/if}} {{/if}}
You can now invite anyone to meet at your favorite Starbucks.
Limited-time feature
27 year old woman | Astoria, NY, USA | Seeking men 27-35 within 10 miles
Active within 3 days
A girl who is actually looking for a nice guy, and is not just saying she is.
I hail from California, and I have been in New York for two years. Before moving here, I worked as a tour guide across America. I drove English and Australian passengers around America's national parks and we camped in all kinds of conditions. Therefore, I love the outdoors and would like someone who does not mind camping and hiking. My job as a tour guide proved to me that I am pretty good at explaining America's history, so I decided to go to NYU and get my masters in History. Last spring I finished my thesis on the history of women in national parks. Currently, I work as a 5th grade History teacher at a charter school in Harlem. I love my job and my charter school's commitment to education reform. I feel as if my job is the most rewarding job that a person can have. There is no greater feeling than helping a student reach their full potential. Sometimes students yell and scream at me and are extremely rude. However, when I do not give up on them, keep pushing them, and then they reach all their academic goals, I cannot help but feel elated that I have made a dramatic difference in this child's life. On a more lighter note, you should ask me out just so you can hear all of my hilarious stories about working with 10 year olds all day. I am looking for someone who wants to join me for Broadway shows, museum exhibits, Shakespeare in the park, jazz performances at the Neue gallery, and civil war days on Governor's island. I also love to throw theme parties where I cook for all my friends. So, you should enjoy dressing up, eating, and board games. I would also like it if you will accompany me to some of my student's performances. I recently went to see them in their school musical, and it was the most adorable thing! Lastly, I need someone who is witty and can banter back in forth with me. I love a good debate, in fact I teach debate as my elective at my school.
Alumni connections , Book club , Camping , Coffee and conversation , Cooking , Dining out , Movies/Videos , Museums and art , Exploring new areas , Performing arts , Political interests , Shopping/Antiques , Travel/Sightseeing , Watching sports , Wine tasting
Walking / Hiking, Yoga
I exercise 1-2 times per week.
I like Cats, Dogs
I grew up with my parents saving dogs from the Springer Spaniel rescue. They currently have four. However, they live on four acres, so it is not an episode of hoarders going on.
Middle of the Road
I like to karaoke, and I am a big fan of the West Village because it has cool piano bars and interesting pubs.
University of California, Los Angeles, Los Angeles, CA
Movies: Annie Hall, The Graduate, Gone with the Wind, The Shining, Music: Jazz Music, Tswift, Michael Buble, Shostakovich, Billy Joel, Classical Food: Indian and Mexican TV shows: Modern Family, Mad Men, Girls, Game of Thrones, The Mindy Project,
I see a play or musical about once a week during the winter because it is easier to get tickets in the winter. I like to travel, and I am going to Eastern Europe this summer. I also like to watch college football, and I like to Karaoke!
Mindy Khaling's book Ken Follet's Fall of Giants Trilogy The New York Times (we read it in our class with the kids each day) I have a subscription to the New Yorker that I read on the train daily.
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What she is looking for
|5'8" (172cms)
|5'11" (180cms) to 8'11" (271cms)
|Body type:
|Slender, About average, Athletic and toned
|Dark blonde
|No preference
|No Way
|No Way
|Social Drinker, Regularly, Moderately
|Education / Teacher / Professor
|No preference
I am a 5th grade teacher in a charter school.
|$50,001 to $75,000
|No preference
|Never Married
|Never Married
|Have kids:
|Wants kids:
|Definitely, Someday
|White / Caucasian
|White / Caucasian
|Christian / Catholic
|Christian / Catholic, Christian / Protestant
|Graduate degree
|Bachelors degree, Graduate degree, PhD / Post Doctoral
UC Los Angeles for undergrad (go Bruins) and NYU for graduate school


text:
JIRA 3.1 and above should not suffer from this problem unless migrating to postgreSQL from another database such as MySQL. Invalid characters otherwise are automatically stripped from imported data.
In older versions of JIRA it was possible to cut & paste text containing control characters into JIRA issue fields. This causes problems, because JIRA's backup format is XML, and XML does not allow for the storage of most control characters.When XML containing control characters is imported into JIRA, the import fails with an error:
To fix this, the control characters will need to be removed from the JIRA backup file. This can be done with the following:
- Download atlassian-xml-cleaner-0.1.jar
- Open a command prompt and locate the XML or ZIP backup file on your computer, ensuring that it is extracted if it's within a ZIP file. In this example, we will use
Run the application with the below:
$ java -jar atlassian-xml-cleaner-0.1.jar entities.xml > entities-clean.xml
This will create a copy of
entities-clean.xmlwith the invalid characters removed.
- Copy the
entities-clean.xmlfile into another directory, rename it back to
entities.xmland create a new ZIP with and the
- Import the new ZIP file, ensuring that it contains both XML files.
If you are seeing an error specifically with 0xffff as the affected character, please use this perl command to fix the file:
Credit for this goes to Jeff Turner who suggested this in the comments below: Re: Removing invalid characters from XML backups
9 Comments
This XML cleaner doesn't strip <ffff> characters. If, after running this XML cleaner you may still get the error:
An invalid XML character (Unicode: 0xffff) was found in the CDATA section.
On my system I was able to fix this with:
perl -i -pe 's/\xef\xbf\xbf//g' entities.xml
Note perl doesn't allow \x{FFFF} in regexps so you have to break it into bytes as above.
I also faced that XML cleaner leaves invalid characters in the XML backup. Since it is not developed any further, it won't be fixed. Another method for the cleaning:
- create the export.zip
- create a test JIRA environment
- import the export.zip into the test JIRA
- log into the test JIRA, create an export.zip again, it will be clean, now you can run the anonymizer
You can Use Notepad++ to clean them out (if file is not too big) using \uffff Search and Replace
"JIRA 3.1 and above should not suffer from this problem. Invalid characters are automatically stripped from imported data"
That's not necessarily true. We're coming from a MySQL backed instance that's moving to Postgresql and we had to pipe the XML through this filter.
Same going from Oracle to Postgresql.
And Oracle to MySQL. :/
The cleaner does not work for us, also removed with Notepad++ and we still have the same error. Opened a ticket with support, will report back once its resolved.
Hi Adam, did you get a resolution on this? I've also had no success stripping the invalid character with either perl or notepad++. I'd appreciate any update on what you've done to resolve this.
Support ended up sorting this out for us, they took a copy of our DB and removed all of the invalid characters.


text:
Police fraud probe over Cockermouth store money
Last updated at 19:43, Thursday, 31 May 2012
A senior member of staff has been sacked from Cockermouth's Lakes Home Centre and police are investigating after allegations that money was taken from the company.
Police have confirmed that a 42-year-old woman has been arrested on suspicion of fraud and bailed while inquiries continue.
It is understood she lives in the town.
Lakes Home Centre managing director Patrick Lister said that a woman had been dismissed from the store's accounts department but would not confirm how much money was involved.
The arrest came after another employee found a discrepancy in the end-of-year accounts for the Low Road store.
The woman arrested had worked at the store since before Mr Lister took over in 2004.
He declined to comment further.
A spokeswoman for Cumbria police confirmed that the force had been contacted by a Cockermouth business regarding allegations of theft of money by an employee.
She said: "As a result, a woman aged 42 was arrested on suspicion of fraud and has been bailed until July 9, while police continue with their inquiries."
First published at 19:23, Thursday, 31 May 2012
Published by http://www.timesandstar.co.uk
More news
- Workington flats plans are approved for second time
- More homes get the go-ahead in Cockermouth
- UPDATED: Planners say no to Bothel solar farm proposal (1 comment)
- Foodbank launches children's lunch packs
- Hair today....gone tomorrow
- Toilet fire ends football tournament on sour note
- Top marks for West Cumbria teachers
- Did you hear Workington's big bang? (19 comments)
- Blue is the colour for Seaton toddler Zayn
- Lakes College learners celebrated


text:
Related Links
Relevant offers
OPINION: The actions of the two Australian DJs who impersonated the Queen and Prince Charles in a prank phone call to London's King Edward VII Hospital where the pregnant Duchess of Cambridge was staying have been universally condemned.
Seldom can those last two words be used literally, but on this occasion the subsequent death - apparently suicide - of mother-of-two Jacintha Saldanha, 46, resulted in exactly that.
What was mild amusement at a childish prank call, coupled with incredulousness that anyone could have been fooled by it, changed into something far more sinister three days later after Ms Saldanha's death.
The humour quickly evaporated at that point, to be replaced by anger. British police are investigating, helped by their Australian counterparts, to reflect how seriously the incident is being treated.
The commercial world showed its distaste at being associated with the actions as advertisers withdrew their support for Mel Greig and Michael Christian's show.
The two radio hosts have gone into hiding and shut down their Twitter accounts, such has been the abuse via the vitriolic world of some social media users.
Radio networks around the world are now reviewing their policies on such prank calls. Many are likely to apply the handbrake for now on the drive to attract listeners in the ratings-driven world of radio by such means.
But there is a wider context to take into account. The two Sydney DJs didn't spontaneously decide to make the call. It was discussed with management, a legal opinion sought, and a recording of the call heard before everyone resolved to play it on air. Unsuccessful efforts were also made to discuss the resulting taped conversation with the hospital.
The next point to ponder is the relatively minor part in the whole incident that Nurse Saldanha actually played. She merely transferred the call to another nurse, which raises the question of just how that could possibly prompt her to take her own life.
In partial mitigation of the foolhardy pranksters, it was not a consequence that could have reasonably been foreseen.
The actions and behaviour of the media is under the microscope as never before. The wide-ranging Leveson Inquiry has brought down several leading figures in the UK and whetted the appetite of those who have suffered at the excesses of the British tabloid press.
That is understandable. Most right-thinking people would recognise the need for change in the way some media behave, although Government regulation is not the answer.
Similarly, turning the heat up on pranksters would be a kneejerk reaction. Advertisers may have deserted the Australian network responsible for the hospital prank - but if there was not an appetite for jackass-style behaviour on air and on television, they would not have been advertising in the first place.
These pranks may forever struggle to pass the taste test, but in this case the tragic epilogue could not have been predicted, and it certainly is not a reason to ban the pranksters.
- Taranaki Daily News
Sponsored links
Duncan Garner: Can the Hurricanes win the Super Rugby title after 20 seasons?
Roger Hanson: Boom and bust cycles
Colin Comber: Landlords want healthy rentals too
Faith in Taranaki: The parable of the geese
Duncan Garner: Digital devices have stolen my life (and wife)
Mulling over the ways to get a good night's sleep
Gordon Burnside: Love your place in the world
Duncan Garner: Deadbeat parents should be in the dock with their kids
Rachel Stewart: You think sheep are dumb?
Editorial: Road rules are for cyclists too
Ross Henderson: Housing NZ should consider he cost of doing nothing
John Sargeant: Too easy to blame Housing New Zealand
The Rilkoff files: The answer to life is a haircut called 48
Taranaki's hairdressers compete for cutting edge
Forever Present care packages supported by artist Brenda Cash
Grant Looker back from the brink of death
Decision over closing of Taranaki Cathedral still a way off
Hawera Rural Women reach 90-year milestone
Google turns NZ streets into a giant game of Pac-Man
'Dare to believe' speech aids Taranaki bowlers
Upsets in opening round of club rugby
Chaos the dog is back from the brink
Losing hair supporting loved-ones
South Taranki iwi receives Crown apology
Taranaki paracyclist chops wood to raise funds
Petitioner says DHB has 'ripped' out service
New Navara continues Nissan ute heritage
Grant Looker back from the brink of death
NZ Post buggie trial a hit with posties and the public
Semifinalists found in Taranaki club rugby
Friendly ghost haunts Okato pub - makes pictures crooked
Ballance award winners celebrate as farm passes wow test
Decision over closing of Taranaki Cathedral still a way off
Forever Present care packages supported by artist Brenda Cash
Duncan Garner: Can the Hurricanes win the Super Rugby title after 20 seasons?
Armed offenders squad fires tear gas in stolen gun search in Waitara, Taranaki
Hawera rugby league hopeful Zion Towers plans for NRL success
Was the weekend's rain welcome relief for your garden/farm?Related story: Yesterday's drizzle no drought breaker for Wellington, Hawke's Bay
Riding Shotgun
with Rachel Stewart
The Rilkoff Files
Matt Rilkoff's perspective of contemporary life
Under the Sky Tower
With Kathryn Calvert
Gordon Burnside
The self-confessed bard of Brixton, offers views on life, politics and Akubra hats.
The Weekend
with Glenn McLean


text:
When it was all said and done, Sierra High's Zach Holmstrom left an undeniable mark on the Timberwolves' swimming program. Sure the boys squad could not fend off challengers for the coveted Valley Oak League title, but sometimes victories are not always measured with championships.
Kara Hall had every opportunity to settle on her outstanding accomplishments for the Sierra High swim program a season ago, but the junior had no such let down, improving on an already successful career by being named the 2010 Bulletin All-Area Swimmer of the Year.
STOCKTON - Amy Haapanen is returning home later this week.
STOCKTON – With overtime not being an option, Stagg High head coach Don Norton decided to leave fate in the hands of his players with just two minutes remaining – Sierra graduate Anthony Hall's hands answered the call.
STOCKTON – Cherie DeJesus wasted little time making an impact in the 20th Annual High School All-Star Softball Classic, driving a pitch over the leftfield fence for an instant 2-0 lead and establishing the inside track to the game's Most Valuable Player honors in the South's 16-7 win over the North Friday at the University of the Pacific's Softball Complex.
STOCKTON – After Matt Barber did his part with a perfectly executed sacrifice bunt in the top of the ninth with runners on first and second with no outs, the South botched a routine out at the plate leading to a pair of North runs in their 6-5 come-from-behind victory over the South Friday at Klein Family Field on the University of the Pacific campus.
STOCKTON - Don Norton, head coaching the North team in the 37th Annual Lions International All-Star Football Classic today at the University of the Pacific, demanded more urgency and intensity out of the players after the first of two practices Friday.
John Bowers and Vincent Herrera were the area's top high school distance runners this past year.
It turns out that Todd Hardcastle has one more high school softball game to coach.
CLOVIS - The clock struck midnight sooner than Vincent Herrera wanted.
Vincent Herrera graduated from Manteca High last week, but he has one more day to sport the school's colors.
When the Manteca Little League major division White Sox fell behind the A's 1-0 in the first inning, the chances of their season ending seemed pretty good.
It was only fitting that when post-season accolades were distributed for Trans Valley League play, that nine Ripon Indians led the charge securing the most spots on the 2010 all-TVL team.
Just like every other athlete in this area that is closing down their season, me and this dude I work for are closing down another campaign.
SACRAMENTO - One came up inches short. Another was fast enough by an eye blink.
East Union had a chit in the karma bank Wednesday night and picked the right time to get it back.
Marcus Montano and his booming right foot are taking the next step.
Comfortable first-round victories allowed the East Union and Manteca girls soccer programs to rest many of their regulars.
It was a return to prominence for the Sierra High baseball program.
TRACY - It has been a long time since the Manteca High softball team has made the playoffs - not in this century, at least.
For weeks now, Manteca coach Neil MacDannald has talked about building momentum for a run at another Sac-Joaquin Section title.
Much of what Jenna Zuniga does on the right wing is cunning and calculated.
Manteca High's girls soccer team padded its program-record setting goal total Tuesday and set another new standard with a 7-0 victory over Vallejo.
Two Valley Oak League champions were crowned at The Corral on Tuesday.
Three league champions fell in the second round of the Sac-Joaquin Section Coed Team Tennis Playoffs on Tuesday.
It's been a season marred by heartbreaking losses for East Union
Ripon High's Nick Sharp and the Ripon Christian boys golf team appear to be peaking at just the right time.
STOCKTON - History was made Monday at Weston Ranch High school.
Before the Rivera sisters exploded on the scene with the force of a three-hit combo, turning East Union into a section heavy, another prolific sister act tormented defenses.
Contents of this site are © 2015 Manteca Bulletin - All Rights Reserved Privacy policy and Terms of service | Contact Us


text:
Petition Update
Discussion about Hermann Nitsch before the "3-day-play"!
Liz von Acquin
Weiler, Austria
Jun 9, 2013 — I'd like to inform you all about the open forum which is going to take place on the 19th of June at WERK 2 in Leipzig, three days before the 3-Tage-Spiel (3-day-play). It will be a discussion about Hermann Nitsch's work. A representative of Leipzig's central theatre (Schauspielhaus Leipzig) will take part. We all hope that we can prohibit this horrible spectacle!
More information at: http://www.werk-2.de/programm/2013-06-19_diskussion_kunst_und_leid-hermann_nitschs_3-tage-spiel_in_leipzip
Trending petitions


text:
Alpha&Βeta Complex is a glycolic and salicylic acid peel. It gently reduces the cohesion of the stratum corneum layer, and encourages the elimination of dead skin cells and produces great results in skin ageing.
Skin Clinic
Online bookings let you pick date/time for your visit.
If you purchase an eVoucher you will need to book with the venue directly.
Our old Wahanda Gift Vouchers can still be redeemed at over 3,000 locations. Use them to pay as you would with cash. Please note, the Wahanda Gift Voucher's successor – the Wahanda Gift Card – can only be used for online purchases.


text:
Video: Tags: chickens
A tag is a word or phrase that helps you find video clips with similar content. View all tags »
Ciao Italia | Chicken Cacciatore | PBS (#1001)
Mary Ann teams up with her mom in her "basement kitchen" to make classic Chicken Cacciatore. 01:53
Ciao Italia | Chicken w/ Orzo, Spinach & Lemon | PBS (#2003)
Chicken w/ Orzo, Spinach & Lemon 06:20
Ciao Italia | Chicken Marsala with Mushrooms | PBS (#2003)
Chicken Marsala with Mushrooms 09:06
Ciao Italia | Chicken Meatballs Florentine | PBS (#1812)
Chicken Meatballs Florentine 06:52
Ciao Italia | How Do You Make Homemade Wine? - Come Si Fa Vino Di Casa? (#1901)
Full Program: Lombardy Style Stewed Beef, Chicken in Padella and a local winemaking supply store are featured. 25:29
Primal Grill with Steven Raichlen | Cutting Chicken Wings
Now presented in amazing HIGH DEFINITION, Primal Grill® with Steven Raichlen Season 3, offers more thrilling grilling based on the worlds oldest and most primal cooking method grilling over live... 00:43
Ciao Italia | Chicken Skillet Supper | PBS (#1901)
Chicken Skillet Supper 02:08
Ciao Italia | Chicken with Cheese | PBS (#1923)
Chicken with Cheese 04:01
Ciao Italia | Chicken Marsala | PBS (#1923)
Chicken Marsala 06:59
Primal Grill with Steven Raichlen | Chicken Trussing
Master Griller Steven Raichlen explains trussing a chicken for grilling. 01:03
Everyday Food | Roast Chicken Tacos | PBS (#605)
Sarah whips up a great meal for under $10. 06:11
Everyday Food | Chicken Cutlets in Herb Butter | PBS (#610)
Lucinda shows you how to make chicken four ways. 06:22
Everyday Food | Chicken Pot Pie | PBS (#603)
John prepares a chicken pot pie. 07:18
The Hypno-Chicken Strikes
Dan Wardell tries not to look at the Hypno-Chicken! 01:05
Sesame Street | Martians Telephone | PBS
The martians encounter a ringing phone. Sesame Street is a production of Sesame Workshop, a nonprofit educational organization which also produces Pinky Dinky Doo, The Electric Company, and other... 02:53


text:
The group is building a multi-racial, cross-class movement that makes racial justice a sustained priority for Jews.
Author Tariq Ali analyzes Syriza's failure to stand up to the troika's demands and proposes a referendum that would place the issue of Greece's eurozone membership on the table.
SpeakOut is Truthout's treasure chest for bloggy, quirky, personally reflective, or especially activism-focused pieces. SpeakOut articles represent the perspectives of their authors, and not those of Truthout.
Dear Esteemed Brothers,
On the way back from my father's village, I've decided to take time to think over topics connected to our common interests. News of your conquests in America first reached us here in the backwater like word of a distant storm. It was in the mouth of Yoweri Museveni that I first heard your name. My friends, I beg you. Listen well. The goat with the keenest ears never ends up with okra in the village stew.
Washington D.C. – Three years ago, newly elected governors in several states decided to outsource economic development functions to "public-private partnerships" (PPPs). Together with a handful of other states' PPPs, these experiments in privatization have, by and large, become costly failures characterized by misuse of taxpayer funds, conflicts of interest, excessive executive pay and bonuses, questionable subsidy awards, exaggerated job-creation claims, lack of public disclosure of key records, and resistance to basic oversight.
The primary problem with weaponized drones is that the weapons murder people. And they murder people in a way that looks more like murder to a lot of observers than other forms of military murder do — such as murder by indiscriminate bombing or artillery or infantry or dropping white phosphorous on people. When President Obama looks through a list of men, women, and children at a Tuesday terror meeting, and picks which ones to murder, and has them murdered, you can call it a war or not call it a war, but it begins to look to a lot of people like murder.
As scientists, physicians, academics, and experts from disciplines relevant to the scientific, legal, social and safety assessment aspects of genetically modified organisms (GMOs), we strongly reject claims by GM seed developers and some scientists, commentators, and journalists that there is a "scientific consensus" on GMO safety and that the debate on this topic is "over".
We feel compelled to issue this statement because the claimed consensus on GMO safety does not exist. The claim that it does exist is misleading and misrepresents the currently available scientific evidence and the broad diversity of opinion among scientists on this issue. Moreover, the claim encourages a climate of complacency that could lead to a lack of regulatory and scientific rigour and appropriate caution, potentially endangering the health of humans, animals, and the environment.
"'Til death do us part," that age-old marriage vow, has always sounded a little, well, non-committal to Confucian ears. In Vietnam, for instance, where I come from, death is not the end of relationships, it only deepens them.
A traditional Vietnamese worships his ancestors. He talks daily to ghosts. Every morning, every night, he lights incense and offers food and drinks to the spirit of his ancestors, prays for protection and asks for advice. If he claims to have seen his grandfather's ghost the night before, few would question him. More likely they would help decipher the meaning of the visitation.
When a young Norwegian tourist is found murdered in the forest of Lake Superior's north shore in Vidar Sunstøl's "The Land of Dreams," one character asks, "Who would want to murder a Norwegian?" The haunting rugged country of the Arrowhead region is the true protagonist and most finely rendered character in this first volume of the mystery writer's Minnesota Trilogy.
With the interim 'debt ceiling/government shutdown' agreement reached last week between the Obama administration and the Teaparty-driven U.S. House of Representatives, the real negotiations on deficit cutting—aka Austerity American Style—are about to begin again.
A long campaign peddling falsehoods around social security and medicare has created a myth that they are facing a budget and fiscal crisis. This claim will be rolled out by Democrats and Republicans alike, to justify cutting programs for the poorest Americans, while handing tax breaks to the richest corporation.
Special-interest groups and political parties spent an unprecedented $24.1 million on television ads and other election materials in state court races in 2011-2012, according to a new report by the Brennan Center for Justice at NYU School of Law, Justice at Stake, and the National Institute on Money in State Politics.
The report, The New Politics of Judicial Elections 2011-12: How New Waves of Special Interest Spending Raised the Stakes for Fair Courts, provides a comprehensive look at 2011-2012 state Supreme Court elections. In the first full election cycle since Citizens United, an explosion of independent spending helped fuel the costliest election cycle for TV spending in judicial election history and posed grave new threats to fair and impartial justice in America.
The news has been buzzing lately about a recent CDC report that confirms a link between the routine use of antibiotics for livestock and growing bacterial resistance. Surprisingly, about 70 percent of all antibiotics used in the United States are given in low prophylactic doses to farm animals in an effort to ward off rampant disease on our mega feedlots. If the CDC is right, this practice is causing people to die in increasing numbers from infections that simply won't respond to treatment anymore. The bacteria have evolved and become resistant to our drugs as fast as we can develop them.
Two road blockades have been erected to prevent the construction of the 1,200 MW Baram Dam. One blockade has been erected near Long Lama, on the shores of the Baram River, with a second blockade near the proposed dam site.
The Baram dam is the fourth dam planned as part of the Sarawak government's plan to build 12 mega-dams. The dam would displace up to 20,000 people and submerge a rainforest area of over 400km2.
Indigenous activists are demanding the immediate halt to planning and construction at the Baram dam and its access road.


text:
DEFINITION of 'Regulation X'
A rule issued by the Board of Governors of the Federal Reserve System (FRS) that governs credit limits granted to foreign persons or organizations for purchases of American Treasuries.
Borrowers who are subject to Regulation X must also prove that the credit they obtain conforms to the limits under Federal Reserve Regulations T (relating to brokers and dealers) and U (banks and lenders).
INVESTOPEDIA EXPLAINS 'Regulation X'
Regulation X is part of the Securities Exchange Act of 1934. It applies to credit secured both within and outside the U.S. Borrowers who can claim permanent residency outside the U.S. and do not obtain or carry purpose credit in excess of $100,000 outside the U.S. are exempt from Regulation X.
Federal Reserve BankThe central bank of the United States and the most powerful financial ...
Federal Reserve RegulationsRules put in place by the Federal Reserve Board to regulate the ...
Broker-DealerA person or firm in the business of buying and selling securities, ...
SecurityA financial instrument that represents: an ownership position ...
Broker1. An individual or firm that charges a fee or commission for ...
Federal Reserve System - FRSThe central bank of the United States. The Fed, as it is commonly ...
What is each party's role in a reverse repurchase agreement?There are two principal parties in a reverse repurchase agreement. The first party, often called the seller, is offering ... Read Full Answer >>
What are some benefits of a mixed economic system?In economic theory, a mixed economic system is a combination of capitalism and socialism, allowing for private property along ... Read Full Answer >>
What average annual growth rate is typical for the banking sector?The banking sector plays an important intermediary role by channeling available funds for productive uses in the economy ... Read Full Answer >>
What are some of the major regulatory agencies responsible for overseeing financial ...There are a number of agencies assigned to regulate and oversee financial institutions and financial markets, including the ... Read Full Answer >>
How does neoclassical economics relate to neoliberalism?While it may be likely that many neoliberal thinkers endorse the use of (or even emphasize) neoclassical economics, the two ... Read Full Answer >>
What regulations exist to protect infant industries?There are far more protections of once-infant and now-dominant industries in the United States than regulations designed ... Read Full Answer >>
Credit Default Swaps: What Happens In A Credit Event?The credit crisis of 2008 prompted important changes to the settlement of credit default swaps.
Credit Card Perks You Never Knew You HadFrom car insurance to concert tickets, be sure to take advantage of whatever your card has to offer.
Credit & Loans
What's On A Consumer Credit Report?A look at the various components and considerations that go into one's credit report and credit score.
Credit & Loans
Getting Your Kids Their First Credit CardPutting some plastic in your children's wallets may actually help them - if you do it right.
Credit & Loans
Take Control Of Your Credit CardsThe plastic in your wallet doesn't have to hurt your finances. Learn how to manage it responsibly.
Credit & Loans
The Importance Of Your Credit RatingA great starting point for learning what a credit score is, how it is calculated and why it is so important.
Credit & Loans
How Credit Cards Affect Your Credit RatingThe average American household has four cards, but does that mean more is better?
Understanding Credit Card InterestPaying these rates can impact your disposable income and your investment returns.
Bonds & Fixed Income
Convertible Bonds: Pros And Cons For Companies And InvestorsFind out why businesses choose this type of financing and what effect this has on investors.
Options & Futures
Combining Credit For A Happy Financial-Ever-AfterA couple's finances may not always be a match made in heaven. Find out when to say "I Do".


text:
Job SearchThe Bravest Interview Question
Tag Archives: persistence
"I'm Tired of Sending Out Resumes and Not Getting Any Responses." by Dr. John Manni, Psychologist
I can't tell you how many times I've heard this from clients and people attending my workshops. It's frustrating to do all that work and not get a single reply. […]
Posted in Advancing, Job Search Tagged don't quit, employment, executive job search, persistence 1 Comment


text:
© The Financial Times Ltd 2015 FT and 'Financial Times' are trademarks of The Financial Times Ltd.
March 19, 2013 9:18 pm
IntercontinentalExchange has tweaked the terms of its planned $8.2bn deal to purchase NYSE Euronext to satisfy regulatory rules surrounding ownership of exchanges.
In a regulatory filing on Tuesday, ICE said it would form a new holding company for both itself and NYSE Euronext, which operates exchanges in the US and Europe. Each share of ICE common stock will be converted into the right to receive one share of the new holding company, which will be listed on the New York Stock Exchange as ICE Group.
The change was made to meet requirements laid down by the Securities and Exchange Commission, which permits parties a maximum shareholding level in stock exchanges of just 20 per cent.
"The terms of the agreement, including consideration to be paid to NYSE Euronext shareholders, are substantially the same," the filing said.
ICE is set to shake up global derivatives trading with its cash and shares deal to buy the New York Stock Exchange. However, ICE, which has emerged only in the past decade via energy and commodity trading, has designs on NYSE Liffe, the European derivatives exchange.
Ownership of it will allow ICE to trade interest rate derivatives, the most widely used derivatives for hedging risk, and create a rival to CME Group and Deutsche Börse as one of the world's largest derivatives exchanges by contracts traded.
The deal comes amid a lull in industry volumes as global low interest rates, little economic growth and greater stability in the eurozone curb investor appetite for trading.
However, under its plan many of NYSE's continental European assets, including the stock exchanges of Paris, Brussels, Amsterdam and Lisbon, are set to be floated in Europe. Dominique Cerutti, deputy chief executive of NYSE Euronext, has emerged as a frontrunner for the new European entity, according to people familiar with the situation.
ICE is finalising its discussions with European regulators ahead of filing its deal proposals to antitrust authorities. It hopes to complete the deal by the end of the year.
Last week Jeff Sprecher, chief executive of the ICE, called for more consolidation among European exchanges as regulation and the euro crisis combined to further the creation of a single market among the 27 euro states.
Exchanges should form a federation "where they accommodate the local markets for listing and capital raising but allow trading to happen cross-border", he said.
Shares in ICE, up nearly 30 per cent this year, fell 0.3 per cent to $159.13. NYSE Euronext shares were down 0.3 per cent to $37.91.
Copyright The Financial Times Limited 2015. You may share using our article tools.
Please don't cut articles from FT.com and redistribute by email or post to the web.
Sign up for email briefings to stay up to date on topics you are interested in


text: If you want to eat something good, not cheap but nor the most expensive in the area, you have to go here ! The waiters are classy, the food is amazing. The soups are making rainbows in your mouth. We were 2 couples and we paid around 55 eur for a full lunch, including the tips ! Awesome awesome awesome... More


text:
By: Severina Snape
"Why, Sir, you find no man, at all intellectual, who is willing to leave London. No, Sir, when a man is tired of London, he is tired of life; for there is in London all that life can afford."
Author: Samuel Johnson
Add your tips, poems, quotes, or title ideas to share with other members.
We'll send you beautiful layouts, innovative project ideas, free classes and more. Subscribe below!
Follow us on your favorite social networks for news and inspiration!


text: Jon Patterson Joined 3 years ago Location New York User Stats 9 videos 8 uploads 1 appearance 1 like 1 following 0 groups 0 channels 0 albums Profile Images Follow Message User Bio Graphic Designer by education—A multi-faceted designer working in multiple medias including, video, packaging, branding, photography and art direction. This vimeo page is collection videos shot over the years. External Links jpdes.com Following Related RSS Feeds Jon Patterson's Videos Jon Patterson's Likes Recently Uploaded + See all 8 videos THE POGO: Concept Video 1 year ago Destinations— Phuket/ Pang Nga 3 years ago Destinations—Krabi p 03 3 years ago Destinations— Krabi p 02 3 years ago Recent Activity 3 weeks ago wooseungji liked THE POGO: Concept Video 1 month ago Atsushi Endo liked THE POGO: Concept Video 1 month ago Craig Campbell liked THE POGO: Concept Video


text:
Women 2.0 Business Competition
ROSE Blog: Rikki's Open Source Exchange
Finalists were announced for the Women 2.0 Business Competition and the top 5 teams will be pitching live tomorrow. It's not too late to register for tomorrow's conference:
Women 2.0 & Stanford Women in Business Conference on Entrepreneurship
Saturday, May 10 10:00am - 4:30pm in Stanford, Californiacomments powered by Disqus
Issue 177/2015
Buy this issue as a PDF
NoScript Flaw
Mozilla's script blocker add-on could be putting malware sites on the whitelist.
RIP SSLv3
The Internet community officially banishes the notoriously unsafe Secure Sockets Layer protocol.
Mate 1.1 Arrives
Popular desktop environment continues the Gnome 2 legacy – with new support for the Gnome 3 toolkit.
US Government Requires HTTPS
The Obama White House has issued a memorandum telling all US government agencies they must use HTTPS for all websites and web communication.
Mozilla Signs Firefox Add-Ons
New program will dial up security for the Firefox browser.
Fedora 22 Arrives
Red Hat's community distro embraces the cloud.
Code.org and College Board Team Reach Out for Talented High School Coders
New partnership will bring more and better CS training to US schools
A Peek Inside TeslaCrypt Ransomware
Criminals offer online help over Tor network
Mumblehard Still Spams
Sophisticated malware is still present on Joomla and WordPress sites around the world.
Launchpad Gets Git
Future versions of Ubuntu's code service will support the popular Git version control system used with Linux and other open source projects.