text:
Page 1
Original Article
Intracellular Phospho-protein Staining Techniques
for Flow Cytometry: Monitoring Single Cell
Signaling Events
Peter O. Krutzik1,2,3and Garry P. Nolan2,3*
1Department of Molecular Pharmacology, School of Medicine, Stanford University, Stanford, California
2Department of Microbiology and Immunology, School of Medicine, Stanford University, Stanford, California
3Baxter Laboratory of Genetic Pharmacology, School of Medicine, Stanford University, Stanford, California
Received 11 April 2003; Revision Received 19 June 2003; Accepted 19 June 2003
Background: Recent advances in intracellular staining
techniques, cytometer technology, fluorescent reagents,
and antibody production have expanded the number of
intracellular antigens that can be analyzed by flow cytom-
etry. Measurement of protein phosphorylation with phos-
pho-specific antibodies has given insight into kinase sig-
naling cascades. However, available techniques for
phospho-epitope staining can differ greatly, making it nec-
essary to understand the differences between the out-
comes when such techniques are applied and to develop
robust and reproducible methods of application.
Methods: Ten different cellular fixation and permeabili-
zation techniques were tested for their ability to provide
phospho-specific staining. Combinations of formaldehyde,
methanol, ethanol, acetone, Triton X-100, and saponin
were used as fixation and permeabilization reagents. Phos-
pho-specific antibodies were labeled with Alexa Fluor
dyes to provide multicolor analysis of different signaling
events simultaneously within individual cells.
Results: Fixing cells with 1.5% formaldehyde followed by
permeabilization in methanol gave optimal results for
pERK, pp38, pJNK, pStat1, pStat5, and pStat6 staining.
Alteration of formaldehyde fixation and methanol perme-
abilization times affected measurements of phosphoryla-
tion induction. Phospho-specific flow cytometric analyses
correlated well with Western blotting, providing cross
platform validation of the technique.
Conclusions: Measuring phosphorylation events by flow
cytometry provides a rapid and efficient way to measure
kinase cascades in individual cells. Stability of phospho-
epitopes in methanol allows long-term storage of samples
prior to analysis. Multiple signaling cascades can be
monitored simultaneously through the use of different
fluorophore labels to determine specificity of ligands or
inhibitors. Application of optimized techniques to heter-
ogeneous cell types such as peripheral blood or murine
splenocytes may allow signaling to be analyzed simulta-
neously in immune cell subsets.
61–70, 2003.
© 2003 Wiley-Liss, Inc.
Cytometry Part A 55A:
Key terms: phosphorylation; kinase; multiparameter; an-
tibodies; cell-based; signaling cascades; Alexa; phospho-
The analysis of cellular signaling pathways has largely
been limited to biochemical manipulations on large num-
bers of homogeneous cells either derived in culture or
purified by cell sorting techniques. Thus, systems with
rare or heterogeneous cell types, such as human periph-
eral blood mononuclear cells (PBMC), murine spleno-
cytes, bone marrow cells, and other primary cells have
largely remained outside the scope of biochemical analy-
sis. In addition, theories on signaling pathways, such as
supersensitivity to signaling thresholds and bimodality,
require analysis of cells at the single-cell level (1–3). How-
ever, recent advances in staining for intracellular epitopes
by flow cytometry have opened these previously obscure
fields of signaling for clearer analysis (4–6).
Many epitopes have been successfully stained within
cells, including viral particles (7,8), immunoglobulins (9),
Contract grant sponsor: National Heart, Lung, and Blood Institute, NIH;
Contract grant number: N01-HV-28183, AI35304.
*Correspondence to: Garry Nolan, Ph.D., Baxter Laboratory of Genetic
Pharmacology, Department of Microbiology and Immunology, Stanford
School of Medicine, 269 Campus Dr., CCSR 3230, Stanford, CA 94305.
E-mail: email@example.com
Published online in Wiley InterScience (www.interscience.wiley.com).
DOI: 10.1002/cyto.a.10072
© 2003 Wiley-Liss, Inc. Cytometry Part A 55A:61–70 (2003)
Page 2
estrogen receptors (10), cytokines (11,12), and specific
proteins such as Bcl-2 (13) and cyclooxygenase (14).
Though staining of static protein molecules can provide
insight into cellular responses to stimuli in long-term ex-
periments, it does not yield information concerning the
dynamic signaling events that occur rapidly after cell stim-
ulation or stress. It is well known that signaling cascades
are often driven by protein phosphorylation on down-
stream effectors that activate the effectors to carry out
their roles. Thus, phospho-specific antibodies that recog-
nize these active proteins might distinguish the "on-off"
state of signaling events.
Several groups have demonstrated staining of phospho-
epitopes for flow cytometric analysis (6). Among the mol-
ecules previously examined are Stat1 (15,16), Stat4 (17),
Akt (18,19), ERK, MEK (5), cJun, p38 (20), and various
others (4). The methods used to prepare cells for staining
with phospho-specific antibodies differed in each case,
but they generally employed a fixation step with formal-
dehyde, followed by permeabilization with alcohols, de-
tergents, or saponin. Because many of the epitopes to be
recognized are novel and might be sequestered in pro-
tected locales within cells, it remains unclear if there
exists a general method (or set of methods) by which
most phospho-epitopes can be stained and analyzed. For
the success of such a protocol, two critical parameters
must be met: 1) the initial cell-fixation step must be rapid
and effective in "freezing" the phosphorylation status of
proteins, and 2) permeabilization steps must allow anti-
body access to their cognate epitopes, in the proper
natured or denatured conformation, for binding. Develop-
ment of a generally applicable protocol would greatly
benefit multicolor, multiparameter analysis. It would also
become important as new phospho-specific antibodies are
Therefore, we took a systematic approach to examining
various intracellular phospho-protein staining protocols in
two model systems, the mitogen-activated protein (MAP)
kinase cascade in Jurkat T cells (21) and the Janus kinase-
signal transducer and activator of transcription (Jak-Stat)
pathway in monocyte-like U937 cells (22,23). Both cas-
cades utilize phosphorylation as a means of activating
downstream effectors. In particular, the MAP kinases, ex-
tracellular regulated kinase (ERK), c-Jun N-terminal kinase
(JNK), and p38 are doubly phosphorylated (on Thr and
Tyr residues); then they translocate into the nucleus to
phosphorylate various transcription factors. Stat proteins
are activated by growth factors and cytokines, such as
IFN-?, IL-4, and GM-CSF. Upon phosphorylation by Jaks,
Stat proteins dimerize and enter the nucleus, where they
bind to DNA directly to modulate transcription.
To further develop flow cytometric approaches to de-
termine phospho-epitope levels, we directly compared
several current methods of cell preparation, including
fixation and permeabilization, staining, and storage. Re-
agents that are commonly used in both immunofluores-
cence and flow cytometry were employed: formaldehyde
and glutaraldehyde as fixatives; and saponin, alcohols, and
detergents as permeabilization reagents (24). We found
that each of these reagents had benefits and drawbacks,
depending upon the circumstances in which they were
employed. We also examined fluorophore choice as a
variable in the labeling of phospho-specific mAbs. We
found that formaldehyde fixation, followed by permeabi-
lization by methanol, provides an extremely rapid and
efficient means to stain a variety of intracellular phospho-
epitopes, including ERK, p38, JNK, and Stats 1, 5, and 6.
We also observed that phospho-epitopes are largely stable
in the conditions defined herein.
The results demonstrate that it will be possible to fur-
ther extend the technique to analysis of cellular subsets
within complex populations or rare cell populations that
are difficult to study biochemically, set up diagnostic flow
cytometric assays for pathologic human samples based on
phospho-protein status, or screen primary cell popula-
tions against molecular libraries to discover novel inhibi-
tors and activators of kinase signaling cascades. We expect
that variations of the techniques employed will allow for
other forms of intracellular staining to be undertaken,
including other post-translational modifications (e.g.,
ubiquitinoylation, glycosylation, methylation, acetylation)
or protein-protein interactions.
The following phospho-specific mAbs used were kindly
provided by BD Pharmingen and Transduction Laborato-
ries (San Diego, CA): phospho-ERK1/2 (pT202, pY204,
and Clone 20A), phospho-p38 (pT180, pY182, and Clone
30), phospho-JNK (pT183, pY185, and Clone 41), phos-
pho-Stat1 (pY701 and Clone 14), phospho-Stat5 (pY694
and Clone 47), phospho-Stat6 (pY641 and Clone 18).
Antibodies were supplied conjugated to Alexa Fluor 488
or 647 dyes (Ax488 or Ax647, Molecular Probes, Eugene,
OR), with fluorophore-to-protein (FTP) ratios of 2 to 4, or
with phycoerythrin (PE) at an FTP ratio of approximately
Chemicals and Cyokines
Phorbol-12-myristate 13-acetate (PMA), ionomycin (IO),
anisomycin, human(h)-IFN-?, hIL-4, hGM-CSF, and BSA
were from Sigma (St. Louis, MO). Ampules of 16% or 32%
formaldehyde (PFA) solutions were obtained from Elec-
tron Microscopy Sciences (Fort Washington, PA) and used
for up to 1 week after opening.
Cell Culture and Stimulation
Jurkat T cells and the human monocytic lymphoma cell
line, U937, were maintained in RPMI 1640 supplemented
with penicillin (100 U/ml), streptomycin (100 ?g/ml),
L-glutamine, and 10% FBS. Subconfluent cultures (?106
cells/ml) were maintained to reduce receptor downregu-
lation and preserve cellular response to stimulation. Cells
growing in complete media were treated with cytokines
or chemical reagents for 10-15 min at 37°C before sub-
jecting them to Western blot or phospho-specific flow
cytometric analysis. Splenocytes were obtained from
Balb/c mice approximately 6-8 weeks of age.
Page 3
Western Blots
Stimulated cells were pelleted and lysed immediately in
lysis buffer (10 mM Tris-HCl, 150 mM NaCl, 1% Triton
X-100, 1 mM Na3VO4, 10 mM NaF, 5 mM EDTA, and
protease inhibitor tablet [Roche, Indianapolis, IN]) for 30
min at 4°C. The cell debris was pelleted and the superna-
tant was diluted with sample buffer, heated, and cooled
before 10-15 ?g lysate was loaded onto 12% SDS-PAGE
gels. Gels were transferred to polyvinylidene flouride
(PVDF) membranes, blocked with PBS containing 0.1%
Tween-20 (PBST) and 1% BSA for 1 h, incubated with
primary antibodies (all at 1:1000 dilution) for 1 h at room
temperature (RT), and washed 3 ? 5 min with PBST.
Rabbit anti-mouse horseradish peroxidase (HRP) (Pierce,
Rockford, IL) was then added for 30 min, the blots were
washed extensively with PBST, incubated with ECL-Plus
(Amersham, Piscataway, NJ), and images were acquired
on a Lumi-Imager chemiluminescent scanner (Roche, In-
dianapolis, IN). Quantitation was performed on Lumi-An-
alyst software (Roche) by integration of relative lumines-
cence units (RLU) in each band. Percent maximum was
calculated by dividing the RLU in a sample band by the
RLU in the maximally-stimulated band.
Phospho-epitope Staining for Flow Cytometry
Optimized protocol. After testing various fixing and
permeabilization conditions, the following protocol was
adopted for its speed, reproducibility, and optimal stain-
ing for all tested Abs. Note that certain Abs may benefit
from using slight variations to this technique (see Results).
Treated and untreated cells were fixed by adding 16%
formaldehyde directly into the culture medium to obtain a
final concentration of 1.5% formaldehyde. Cells were in-
cubated in fixative for 10 min at RT and pelleted. They
were then permeabilized by resuspending with vigorous
vortexing in 500 ?l ice-cold MeOH per 106cells (this is an
approximate number, and more or less methanol can be
used as long as evaporation is not significant) and incu-
bated at 4°C for at least 10 min. At this point, cells can be
stored at -20°C for several weeks with minor loss in signal
strength. Cells were washed twice in staining media (PBS
containing 1% BSA) then resuspended in staining media at
0.5-1 ? 106cells per 100 ?l. Optimal concentrations
(typically approximately 50 ng) of fluorophore-specific
mAbs were added and incubated for 15-30 min at RT. The
cells were washed with 15 volumes of staining media and
pelleted. Finally, samples were resuspended in 100 ?l
staining media and analyzed.
Other protocols. Ethanol and acetone were substi-
tuted directly for methanol in the above protocol. When
Triton X-100 was used as permeabilization reagent, it was
included at 0.1% or 0.5% in staining medium and added to
cells that had been fixed in formaldehyde. After 10 min,
the cells were washed twice with staining medium, then
stained as above. When saponin was used as a permeabi-
lization reagent, formaldehyde fixed cells were treated
with 0.1% or 0.5% saponin in staining medium for 10 min,
washed, and then stained in the same saponin-containing
buffer. After staining, cells were washed with 0.1% or
0.5% saponin. All samples were resuspended in staining
medium prior to analysis.
Analysis. Samples were analyzed on a FACSCalibur
cytometer (BD) equipped with 488 and 633 nm lasers, and
emission filters for FITC, PE, Cy5-PE, and APC. The cytom-
eter was routinely calibrated with Calibrite beads (BD).
Stained samples may be stored for up to 16 h at 4°C before
analysis without a significant loss in intensity. Fold change
was calculated by dividing the median fluorescence inten-
sity (MFI) of the stimulated sample (MFIstim) by that of the
unstimulated sample (MFIuntsim), and fold change was de-
fined as MFIstim/MFIunstim. Paired t-tests were performed to
compare fixation conditions and fluorophores, with sig-
nificant values defined as P ? 0.05. Percent of maximum
was calculated by dividing the MFI of each sample by that
of the maximally stimulated sample, i.e., percent max ?
(MFIsample/MFImax) * 100.
Stimulation Conditions for
protein Induction
To evaluate various techniques for their ability to effi-
ciently and reproducibly measure protein phosphoryla-
tion events inside cells, stimulation conditions that pro-
duced induction of kinase cascades had to be determined.
Jurkat T cells and U937 monocytic cells were tested for
their response to various stimuli, including PMA, PMA and
ionomycin, anisomycin, IFN-?, IL-4, and GM-CSF (Fig. 1).
After stimulation, cells were split for either Western blot-
ting or phospho-protein flow analysis with pERK, pp38,
pJNK, pStat1, pStat5, and pStat6 mAbs. Figure 1 shows the
results of flow cytometric analysis with the optimized
protocol outlined in this paper, in order to determine the
validity of the stimulations used. Cells were stimulated,
fixed with 1.5% formaldehyde (PFA), permeabilized with
ice-cold methanol, then stained at room temperature with
the indicated fluorescently-labeled phospho-specific mAbs.
As can be seen in Figure 1, phospho-protein analysis by
flow cytometry correlates well with Western blotting. In
Jurkat cells, PMA (with or without ionomycin) stimulates
PKC, which is known to be upstream of ERK; activation of
ERK was confirmed by both Western and FACS analysis. JNK
and p38 respond to various cellular stresses and are both
known to be activated in response to anisomycin, a protein
translation inhibitor. Intermediate levels of pp38 are evident
with PMA and PMA/ionomycin stimulation by both experi-
mental methods. In U937 cells, the expected cognate signal-
ing pathways were activated via the cytokines applied. In
particular, IFN-? and IL-4 selectively signaled through pStat1
GM-CSF, but intermediate levels were observed after IFN-?
and IL-4 stimulations. Thus, it is clear that flow cytometric
analysis of phospho-proteins is able to measure on-off states,
as well as graded levels of activation.
Analysis of Fixation and Permeabilization
Techniques for Phospho-protein Analysis
After finding stimuli that consistently induced phospho-
epitopes in the two cell lines tested, we proceeded to test
Page 4
a panel of fixation and permeabilization methods for their
ability to show the greatest fluorescence ratio of treated to
untreated cells (Fig. 2A). Two general techniques were
employed. Methods 1-3 used organic solvents to dehy-
drate cells and to simultaneously fix and permeabilize
them. Methods 4-10 first employed a fixation step with
formaldehyde (10 min) prior to permeabilization with
organic solvents, Triton X-100, or saponin. Glutaraldehyde
was also tested as a fixative, but due to increased back-
ground and nonspecific mAb binding, we did not include
FIG. 1. Flow cytometric analysis of phospho-epitope levels correlates to Western blotting techniques. A: Jurkat cells were either unstimulated or treated
with PMA (50 nM), or PMA (50 nM) and ionomycin (IO, 1 ?M) or anisomycin (2 ?g/ml) for 10 min at 37°C. Cells were then divided and lysed for Western
blot analysis or fixed with formaldehyde and permeabilized with methanol for flow cytometric analysis (using optimal techniques, as discussed in the text)
with pERK1/2 Alexa (Ax) 488, pp38 Ax647, or pJNK Ax647. Unconjugated mAbs were used for Western blots. Unstimulated samples appear as open traces
in the FACS plots. B: U937 cells were left unstimulated or treated with IFN-? (50 ng/ml), IL-4 (10 ng/ml), or GM-CSF (10 ng/ml) for 10 min at 37°C. The
cells were split as above, and analyzed with pStat1 Ax488, pStat5 Ax488, and pStat6 Ax647. Note that there is a strong correlation between Western blotting
and flow cytometric analysis with intermediate levels of pp38 and pStat5 discernible by both methods.
FIG. 2. Differences in fixation and permeabilization techniques suggest an optimal protocol. A: Ten fixation/permeabilization methods (perm methods)
were tested for their ability to show maximal shift in phospho-epitope staining in response to stimulation of the model cell lines. The FACS histograms
show one representative experiment in which U937 cells were treated with IFN-? (50 ng/ml) for 10 min, then fixed with 1.5% formaldehyde (PFA)
(methods 4-10), permeabilized with various reagents for 10 min, and stained with pStat1 Ax488 for 30 min. Open histograms represent unstimulated cells;
filled plots indicate treated cells. Note that there were large differences for pStat1 staining between organic solvents (methods 4-6) and typical
permeabilization reagents, such as saponin and Triton X-100 (methods 7-10). B: Summary of fold changes measured with phospho-mAbs by the 10
permeabilization methods. U937 cells were stimulated with IFN-? (50 ng/ml) and GMCSF (10 ng/ml) for 10 min, and stained with pStat1 Ax488 and pStat5
Ax647. Jurkat cells were exposed to PMA (50 nM) and ionomycin (1 ?M) for 10 min, and analyzed for pERK1/2 (Ax488) and pp38 (Ax647) levels. Data
represent the average of three independent experiments, in which the trends shown were extremely consistent. The right axis represents fold-change of
pStat1. Fold change ? MFIstim/MFIunstim.
Page 5
those results here (data not shown). When using Triton as
a permeabilization reagent, cells were treated with this
agent, then washed with staining medium and stained in
the absence of Triton. This technique decreased the num-
ber of cells lost to detergent lysis throughout the protocol
and improved signal intensity. When saponin was used, it
was kept at a constant concentration in staining buffers at
all times, and it was also present in wash buffers.
Figure 2B shows the results obtained by the various
techniques with pERK, pp38, pStat1, and pStat5. Fold
changes (MFIstim/MFIunstim) were calculated so that FACS
data could be visualized in graphical format for compari-
son. Method 4, which employed a 10 min formaldehyde
fix at RT, followed by methanol permeabilization for 10
min at 4°C, showed the best results for pERK, pStat5, and
pStat1 and was comparable to methods 3, 5, and 6 for
pp38. The combination of formaldehyde with organic
solvents (methanol, ethanol, or acetone) was significantly
better than organic solvents alone or formaldehyde-deter-
gent combinations for pErk, pStat1, and pStat5 (P ? 0.05).
For pStat1, formaldehyde in combination with methanol
provided a larger fold change than formaldehyde with
ethanol or acetone (P ? 0.05). Analysis of pp38 was
roughly equivalent among the formaldehyde-solvent or
solvent-alone methods. Triton and saponin were largely
ineffective as permeabilization reagents in the context of
these mAbs, except for pp38, in which they provided
acceptable staining intensities. It appeared, however, that
saponin, a natural product isolated by extraction, differed
in effectiveness due to manufacturer lot variations and
was inconsistent under our test conditions. In these ex-
periments, formaldehyde fixation followed by methanol
permeabilization was robust and consistent between ex-
For pERK, pp38, and pStat5, the use of organic solvents
alone (methods 1-3) provided good staining. Application
of a one-step fixation/permeabilization protocol is attrac-
tive because of its technical ease; however there are two
major advantages to fixing with formaldehyde prior to
permeabilization. First, formaldehyde appears to stabilize
overall cell structure when treating later with methanol
(Fig. 3). When cell lines are the target of analysis, this may
not be of concern. However, in complex populations
such as murine splenocytes, scatter properties may be a
useful diagnostic tool in analysis, and therefore efforts
should be taken to maintain scatter properties. As shown
in Figure 3, methanol alone led to a large increase in side
scatter that was largely eliminated by prior treatment with
formaldehyde. Formaldehyde itself does not significantly
alter scatter properties. Treating with triton or saponin after
fixing caused a small decrease in side scatter. Work is under-
way to establish effects on surface markers-in most cases
we observe no changes of binding of antibodies to surface
epitopes, but we have in certain cases seen complete loss
of binding that is clone- and epitope-dependent (data not
shown). The second advantage of using formaldehyde
prior to organic solvent treatment is technical-formalde-
hyde fixation prevents the clumping that is typically seen
when cells are immediately exposed to alcohols.
Optimization of Fixing Percentage,
Time, and Temperature
The formaldehyde/methanol method (method 4) was
further tested and optimized to determine the parameters
that are critical to experimental success. The first aspect
that was examined was the PFA fixation step. Initial ex-
periments used PFA at a concentration of 1.5% for 10 min
at 37°C (5). PFA concentrations from 0.5% to 3% (for 10
min) were tested, with 0.5% showing slightly lower stain-
ing levels than 1.5% and 3%, respectively (data not
shown). Fixation was performed by directly adding PFA to
the culture media, to ensure rapid freezing of phosphor-
ylation levels within the cells. Because cells must be re-
moved from incubators to add PFA, we tested whether
FIG. 3. Formaldehyde maintains scatter prop-
erties of permeabilized cells. Murine splenocytes
were treated with red blood cell lysis buffer, then
placed in RPMI supplemented with 10% FBS for
1 h. The cells were then left untreated, perme-
abilized directly with methanol, or fixed with
formaldehyde (PFA) and then permeabilized
with various reagents. A gate was drawn around
lymphocytes in the unfixed sample to serve as a
reference for effects on scatter caused by fixa-
tion/permeabilization. Numbers indicate per-
centage of cells in the lymphocyte gate.


text:
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subset: commoncrawl


text:
Owners of area fitness centers and Gainesville's mayor opposed a measure Tuesday to fill the meeting rooms at the Frances Meadows Aquatic and Community Center with fitness equipment.
Gainesville City Council members on Tuesday sided with residential property owners who organized to keep business interests from infringing on their neighborhood.
The South Hall Community Center will close at 9 a.m. to allow the Hall County Parks and Leisure Services staff to transition into the new Mulberry Creek Community Center.
The Georgia Department of Transportation has set a final round of public hearings on plans to allow solo commuters to ride - for a fee - in Interstate 85 car-pool lanes in Gwinnett and DeKalb counties.
Two children are among the 30 potential witnesses listed in an indictment of a Gainesville teen charged in an Oct. 5 fatal shooting.
More than 15 years ago, Good News Clinics grew out of the need local doctors were seeing in their own practices.
Enrollment in the Gainesville and Hall County school systems grew slightly this school year, which superintendents say is a good thing following lower than expected enrollment growth last year.
The Hall County Sheriff's Office is investigating a stabbing in North Hall that left a Flowery Branch man hospitalized, according to Sheriff's Col. Jeff Strickland.
Two Dawsonville teens were charged with burglarizing North Hall High School after the father of one of the suspects recognized her from a surveillance photo.
The Gainesville school board allocated $205,000 to reroof and clean the old gym at Gainesville High School and soon will decide whether it will build a new Fair Street IB World School.
The Times is interested in hearing about area celebrations and activities planned next week marking Veterans Day, which is Nov. 11.
The Hall County school board may adopt a hybrid schedule for its high schools that would allow the system to operate with about 35 fewer teachers next school year.
Leaders of the Gainesville and Hall County school systems fielded questions from Rotary Club members Monday as part of the civic group's State of the Schools presentation.
Health officials are still investigating the cause of an outbreak of symptoms that led to the closure of an elementary school in Buford on Friday.
If you're feeling a bit water-logged after last month's rains, you should get a chance to dry out this week.
Page 1150 of 1629
When Wendy Boswell said goodbye to her foster daughter last month, she literally ached with grief.
As she talks about the joys of working with foster children, Tyne Jackson can't help crying a little.
The removal of a child often results in three types of trauma, according to Hall County Juvenile Court Judge Lindsay Burton.
At 8:30 a.m., the briefs are organized and the coloring books are gathered. The robe goes on for Hall County Juvenile Court Judge Lindsay Burton.
Foster care is in crisis in Hall County.
Page 10 of 10
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Kelly Thomas Beating: Veth Mam Had Earlier Clash With Fullerton Cops That Was Caught on iPhone Video, And Now He's Suing
On a night when Fullerton hired Michael Gennaco, head of the Los Angeles County's Office of Independent Review, to look into the death of homeless man Kelly Thomas following a clash with Fullerton cops, police in the Orange County city confirmed to City News Service that it has launched a probe into an earlier confrontation involving city officers that was caught on video.
In this October confrontation a man named Veth Mam was arrested for interfering with police after he accused cops of roughing up his friend. He was acquitted in part because the whole thing was captured on video.
An attorney indicates the iPhone video contradicts what cops said happened, and now Mam plans to sue.
News of that confrontation was first explained at the O.C. law blog Flock of Legals and echoed at our sister publication OC Weekly after KNX 1070 Newsradio first reported the existence of the video and we followed up.
Mam's Attorney, Garo Mardirossian, tells CNN:
We see the officer Kenton Hampton grab Mr. Mann, throw him on the ground, knee his back. And Mr. Mann offered no resistance. He was not committing a crime. He was doing nothing wrong. He was just videotaping a police officer's interaction with one of his friends.
Co-counsel Veronica R Guzman writes at Flock of Legals:
Fortunately, our client had video of the entire incident. Video that our client caught on his iPhone!!! With video in hand which clearly supported our client's case - that he didn't do it - we were sure this case would be dismissed. But no, it wasn't. Our case went to trial and our client was vindicated with all not guilty verdicts!
The case went to trial because the officer who alleged in his report that he pulled our client off the arresting officer's back, claimed that the incident occurred before the video started recording. Unfortunately for him, we made his perjury clear to the jury.
She writes that, lucky for Mam, even as he went down in his clash with cops, a bystander grabbed the iPhone and continued filming.
One of the officers in that confrontation appears to be Kenton Hampton, who was also reported to have been involved in the Thomas beating, according to our reading of reports, including OC Weekly's.
Mardirossian says he's going to file a federal suit against the Fullerton PD today.
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Forbes ranks Lightning 23rd on NHL franchise value list
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Two things. It is my understanding that the Forbes estimates include the debt of any arena costs as well. Including renovations. Vinik just dumped nearly 50M into that. If thats true then they really made quite a bit of profit from actual hockey.
As for the fans, the things they rate them on are ridiculous. Prices of things? Like the fans have control over that. And since when are cheap things (read: not ridiculously price gouged things, Im looking at you Toronto), a bad thing? Attendance we were 96% full and in the middle of the pack last year, on a team that didnt make the playoffs. And TV ratings? That would completely depend on the TV market available. Its just very arbitrary uncontrollable things for them to rank the actual fans on
I read this yesterday. Other than providing grist for the BOH relocate mill, it really doesn't say anything pertinent to or about the Lightning fan base. As we are all aware down here, ST prices went up after last season, and sales were still good. Well, at least before lockout chatter really started penetrating. And TV ratings last season were up 60 % last season. Very little mention of how things are improving and total failure to take into account the dark years of OK Hockey. As for social media and merchandising, meh. We're a small market in a transplant state with people who come down here with their lifetime sports allegiances in tow.
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The Weinstein Company Nabs Rights to Sundance Hit FRUITVALE
According to Deadline, The Weinstein Company is entering final negotiations to acquire Sundance sleeper hit FRUITVALE. The deal will reportedly set the company back just over $2 million for a wide theatrical release.
The film, which premiered at the Sundance Film Festival on Jan. 19, immediately sparked a bidding war between Fox Searchlight, Paramount Pictures, Focus Features, and CBS Films. Weinstein reportedly won out.
FRUITVALE, directed and written by Ryan Coogler, tells the true story of Oscar, a 22-year-old Bay Area resident who crosses paths with friends, enemies, family and strangers The Day before he was shot to death by Oakland police officers.
Michael B. Jordan (FRIDAY NIGHT LIGHTS,) Octavia Spencer (THE HELP,) Melonie Diaz, Ahna O'Reilly, Kevin Durand, and Chad Michael Murray star in the film.
The film was produced by Forest Whitaker and his Significant Productions partner Nina Yang.
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| O-T Lounge
| Dog friendly restaurants in Baton Rouge? - Updated with listing - Will Cover on 6/30/15 at 12:02 pm
I visit Dallas and Austin quite frequently and see people with their pets sitting outside at restaurants. Can't say that I have seen the same in the Baton Rouge area. Is there an ordinance that I am unaware of that prevents people from bringing their pets with them outside at a restaurant? ...View Topic - Replies (28)
| Movie/TV Board
| Netflix, switching profiles? - Will Cover on 6/29/15 at 8:02 am
Just signed up for Netflix last night. Set up each individual profile for me, wife, daughter # 1 and daughter # 2. Daughter # 1 and daughter # 2 have their own profiles set to "Kids 12 & Under." They are currently watching Netflix on their iPads. Mom and dad have our profiles set to "Adult...
Posted on Movie/TV BoardView Topic - Replies (2)
| Money Talk
| Changing companies. A look at benefits. WWYD? - Will Cover on 6/28/15 at 2:27 pm
Current company, I pay annually $4,272.58 for medical insurance (family), $541.32 for dental (family) and $705.34 for long term disability (self). Not sure what my company contributes to medical and dental coverage, since I am not exposed to a total compensation statement. Out of pocket maximum fo...
Posted on Money TalkView Topic - Replies (2)
| O-T Lounge
| Belle of Baton Rouge hotel - has service truly deteriorated? - Will Cover on 6/28/15 at 1:56 pm
I haven't been in years, however at one point I do remember it being a decent downtown hotel. Have they failed to keep up with the recent influx of hotel chains that are now present and have set a new standard? [img]http://i.imgur.com/j7ZmlRK.jpg[/img] [img]http://i.imgur.com/9lno4QC.jpg[/i...View Topic - Replies (6)
| O-T Lounge
| Is Montego Bay, Jamaica a family destination place? - Will Cover on 6/23/15 at 1:55 pm
Just realized that Southwest now flies here (and maybe they have for a while?). I know nothing about this place and while I could research online, I'd rather hear from posters that have been there. I have two children, ages 9 and 6 and ideally, we can stay for free at a Hilton property and fly...View Topic - Replies (22)
| O-T Lounge
| New Orleans Police Officer shot dead - Will Cover on 6/20/15 at 11:11 am
Details are just now coming from WWL. Transporting an arrested suspect and suspect got a hold of a gun and shot officer in head. http://www.wwl.com/pages/21658865.php...View Topic - Replies (26)
| Food and Drink
| Pre-Father's Day grilling, another reverse sear thread - Will Cover on 6/19/15 at 4:13 pm
This is my 3rd attempt to do this. First time, the results were perfect (posted on here). Second time, (not posted on here), results were mediocre. I overcooked my steak. Third attempt, you be the judge. Ingredients: 1 Porterhouse, Thick Ass Sea Salt Fresh Cracked Pepper Beer Tim...View Topic - Replies (10)
| Tiger Rant
| Relax, LSU is a 2 to 1 favorite over TCU tomorrow - Will Cover on 6/17/15 at 5:45 pm
:geauxtigers: LSU - 200 TCU + 170 8.5 over - 110 8.5 under - 110 In tonight's action, Florida - 210 vs. Miami Florida + 170. ...View Topic - Replies (13)
| O-T Lounge
| Would you pay for a missed doctor's appointment? - Will Cover on 6/16/15 at 11:04 am
Cliff notes. Cost is minimal, dealing more with principle. Place of business called my wife to confirm her doctor's appointment. It was a live person. Wife arrived at her doctor's appointment. Unfortunately, the person that reminded my wife of her appointment provided her with the wrong...View Topic - Replies (30)
| Tiger Rant
| LSU 3 to 1 favorite to win today in Omaha - Will Cover on 6/16/15 at 9:16 am
LSU - 300 CSF + 200 Total combined runs, 8 over | under - 100 In other action, TCU - 105 Vanderbilt - 115 ...View Topic - Replies (10)
| Tiger Rant
| LSU 2 to 1 favorite to win tomorrow vs. TCU - Will Cover on 6/13/15 at 6:25 am
LSU - 200 / TCU + 170 Arkansas - 120 / Virginia + 100 Florida - 165 / Miami + 145 Vanderbilt - 250 / CS Fullerton + 210 :geauxtigers:...View Topic - Replies (2)
| Outdoor Board
| LineX vs. Rhino Lining - Will Cover on 6/9/15 at 2:05 pm
From what I have read, it appears that installation is the biggest determining factor for both products. [u]LineX[/u] - Some people dislike the rough Line-x surface because it's "harder". It can be hard on the knees while moving around in the bed and it can damage your cargo (finished wood s...
Posted on Outdoor BoardView Topic - Replies (6)
| Food and Drink
| Is there a better tasting chicken wing? - Will Cover on 6/7/15 at 8:09 am
Previous favorite, TJ Ribs. Sauce on the side. Hard to beat a whole wing with a smokiness flavor. My new favorite, albeit much smaller and different, however just as tasty are the wings at Table Kitchen & Bar Baton Rouge. While I limited myself to 5 last night (sharing), I would have been...View Topic - Replies (8)
| Food and Drink
| Non-traditional seasoning for steaks - Will Cover on 6/6/15 at 3:30 pm
I enjoy a great, traditional (kosher salt and fresh cracked black pepper) steak just as much as anyone else. But every once in a while, I like to do something different with seasonings. Thought I'd share (taken from Food & Wine Magazine). Seasonings work great on a large Porterhouse or T-...View Topic - Replies (6)
| Tiger Rant
| LSU baseball a huge favorite this weekend (and other noteables) - Will Cover on 6/5/15 at 7:38 am
Of course anything can and will happen. However, LSU - 350 vs. ULL + 250 this Saturday. This means, if you were to wager on LSU, you would have to wager $35 to win $10 or $350 to win $100. If you were to wager on ULL, for every $10 you put up, you would win $25 or every $100.00 you put up, you ...View Topic - Replies (4)
| O-T Lounge
| I've found a better online customer experience than Amazon - Will Cover on 6/4/15 at 11:42 am
And while it is more specialized, for pets only, their customer service is phenomenal. [link=(http://www.chewy.com)]Chewy[/link] I ordered a dog crate over 5 weeks ago and just found out that is too large for my dog and our room. Wife doesn't like it, but dog just recently started sleeping ...View Topic - Replies (8)
| O-T Lounge
| Southeast Baton Rouge. What happened? - Will Cover on 6/3/15 at 9:39 am
[quote]EBRSO arrests 13 in warrant roundup at apartment complex[/quote] [img]http://wafb.images.worldnow.com/images/7959308_G.jpg[/img] [quote]The East Baton Rouge Sheriff's Office arrested 13 people Tuesday in the St. Jean Apartments.[/quote] [quote]The warrants and arrests ranged from tra...View Topic - Replies (14)
| O-T Lounge
| Hilton HHonors reward members - Will Cover on 6/3/15 at 7:05 am
I'm looking at possible places to stay for vacation (can be either during peak or off peak season) and am curious as to where you like to spend your points? Generally speaking, when using points, I will only consider Hilton, Waldorf, Curio (limited number due to availability) and Conrad (limite...View Topic - Replies (13)
| Money Talk
| 5 Ways to Get Your FICO Score for Free - Will Cover on 6/1/15 at 6:54 am
1. Credit Counselors 2. Credit Cards 3. Auto Loans 4. Credit Unions 5. Student Loans [link=(http://www.dailyfinance.com/2015/05/28/5ways-get-free-fico-score/)]Full story, including the explanations of the 5 ways to get your FICO score for free[/link] [u]Alternative Credit Scores[/u] [qu...
Posted on Money TalkView Topic - Replies (0)
| O-T Lounge
| Blood in my dog's urine - Will Cover on 5/27/15 at 1:15 am
New Doberman puppy, only 3 months old. Yesterday and more so today, she has had the urge to urinate more frequently (as in several times) while outside. Didn't think too much of it as I thought maybe she is getting used to our house as her home and marking her territory. Tonight, I awoke t...View Topic - Replies (21)
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Customizing Your Communications Suite Deployment
Create customized code and interfaces for Communications Suite.
Create customized code and interfaces for Communications Suite.
Programming with the MTA SDK
Using Java Message Queue
Using the Event Notification Service (ENS)
Customizing Convergence 1.x and 2
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1971 PONTIAC GTO COUPE. . . . . REAL 242 VIN CODE. . . . GOLD PAINT ON A STRAIGHT BODY. . . .... More
This gorgeous 1970 GTO was recently fully restored to its current glory by the previous owner and his grandson. It is painted the correct... More
A rare factory triple black with rare factory tri-power and a 4-speed manual transmission. It has PHS documentation, protecto-plate, order sheet and delivery invoice.... More
This 1969 Pontiac GTO Ram Air IV has a 400 C.I. Ram Air IV (370 HP) V8 (Numbers Matching), Turbohydramatic 400 Transmission, Safe T-Trac... More
This 1965 Pontiac GTO Hard Top Coupe features a 389 V-8 8cyl Gasoline engine. It is equipped with a 3 Speed Automatic transmission. The... More
1967 GTO, Gulf Turquoise, Black Interior, Original Automatic Transmission and Rebuilt Original Motor with 3,000 miles. Very clean and very original, prior owner had... More
1965 Pontiac GTO Convertible, shown in attractive Mayfair Maize with Black Interior and Black Soft-Top and 15,331 miles showing on the odometer. 389ci Tri-Power... More
*PHS documents code 242 real GTO 2 door *389 Tri-Power V-8, automatic transmission *Headers with dual exhaust *Power steering & power brakes *Super nice bucket seat interior with... More
This 1964 Pontiac GTO Convertible 1964 Pontiac GTO Convertible features a LS9 6.2L SC 638 HP ZR1 8cyl Gasoline engine. It is equipped with... More
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This is a nicely preserved GTO that runs and drives great! The car has had 1 repaint and is very original. The interior is... More
1968 Pontiac GTO, Numbers Matching 4 Speed ~ 1968 Motor Trend Car of the Year - the Pontiac "GTO". The inertial force that started the supercar... More
For sale in our Orlando showroom is a 1968 Pontiac GTO. Every once in a while we come across a vehicle with an amazing... More
Award Winner 1992 Pontiac Nationals 2nd place 2014 Murrieta Car Show top 40. This car is immaculate. The milege is 55,680 with past history of... More
Over 2,500 hours in this incredible low mileage build done by a master fabricator, Mike Wilson of Wilson's Autobody in Altoona, PA. This GTO... More


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Celebrity Central / Top 25 Celebs
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Published: Sunday September 07, 2008 20:05 PM EDT
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- Facebook Photo Helps Mother and Son Reunite, 15 Years After He Was Kidnapped: 'It's a Miracle'
- Kim Kardashian West: 'I Don't Do Fillers or Botox When Pregnant'
- Kourtney Kardashian Steps Out as Photos Surface of Scott Disick Getting Cozy with Another Woman


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From the Top, favorite dances from 12 years of Vivid Motion.
St. Lawrence Arts and Community Center, Munjoy Hill
Friday 7/24 - 7:30pm
Saturday 7/25 - 2:30 & 7:30
Sunday 7/26 - 2:30pm.
Tickets $13 adults, $6 kids under 13.
Sponsored by St. Lawrence Arts, Yelp of ME, and Gorham Self Storage.
The board thanks everyone who came to Peter Pan and helped raise $1,000 for the Center for Grieving Children.
Next Board Meeting is Sunday, June 14.
If you're interested in attending, send us an email.
Company classes will pick back up in the Fall.
Check out From the Top! July 24-26
Have a great summer!
Vivid Motion Inc. is a 501-c-(3) non-profit organization started by local dancers in Portland ME who wanted to perform and share their love of dance with the community.


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VIEWS: 7 PAGES: 14 POSTED ON: 7/28/2012 Public Domain
Show Me the Blog Social Media & Blogging Conference Schedule 8:00 am – 9:00 am: Registration, Breakfast, Opening Remarks Danyelle Little, Founder & Organizer, Show Me the Blog Social Media & Blogging Conference 9:00 am – 9:20 am: Linda Doty, JustLinda.net, Speaker ―Laughing Out Loud (LOL): Social Media & the Comedy Within‖ 9:20 am – 9:45 am: Roshini Cope, Glamazini.com, Speaker ―The Ten Commandments of a Successful You Tube Channel‖ 9:45 am – 10:15 am: Marijean Jaggers, Vice President, Standing Partnership, Speaker ―Ambassador You: Integrating Social Media into Business Communications‖ 10:15 am – 10:30 am: BREAK 10:30 am– 10:50 am: Melissa Gilliam, Marketing Director, YMCA Trout Lodge & Camp Lakewood, Speaker ―Non-Profits: The Utilization & Implementation of Social Media & Social Networking to Spread Message‖ 10:50 am – 11:20 am: Panel, ―Mommy Blogging: From Bottles to Blogging to Business‖ Robyn Wright, RobynsOnlineWorld.com Stefany Thode, ToBeThode.com Heather Vaughn, WistfulWrists.com Melody Meiners, STL Family Life Moderated By: Jessica Benton, ThatTechChick.com 11:20 am – 11:40 pm: Vernon Ross, Ross Public Relations, Speaker ―Gaining Sponsorship for Your Blog, Brand, or Cause‖ 11:40 am – 12:00 pm: Jeff McIntire-Strasburg, Sustainablog.com, Speaker ―How to Incorporate Green Blogging into Your Social Media‖ 12:00 pm – 1:00 pm: LUNCH- Sponsored by BlackSportsOnline.com 1:00 pm – 1:30 pm: Robert Littal, BlackSportsOnline, Speaker ―How to Take Your Blog from Free Agent to First Draft Pick‖ 1:30 pm – 1:50 pm: Jim Barnthouse, Habanero, Speaker ―How to Brand Yourself & Your Blog‖ 1:50 pm – 2:15 pm: BREAK 2:15 pm – 3:00 pm: Panel, ―Show Me the Blog (And the Money!): The Real Jerry McGuire" s of St. Louis Social Networking. Monetize Your Website Chris Reimer, Scorch Agency, RizzoTees.com Robert Littal, BlackSportsOnline.com Vernon Ross, Ross Public Relations Moderated By: Todd Jordan, ToddJordan.com/TheBroadBrush 3:00 pm – 3:45 pm: Panel, ―It's Not Just About the Food! Social Media, Food Blogging, & Broadening the Menu "Online‖ Abby Schwarz, Community Manager, Yelp St. Louis, Yelp.com Angela Ortmann, STLwinegirl.com Stef Pollack, CupcakeProject.com Troika Brodsky, Communications Director, Schlafly Beer Moderated By: Wilma Jean Levine, WilmaJean.com 3:45 pm-4:00 pm: BREAK 4:00 pm – 4:25 pm: Panel, Blogs That Break the Mold: Making Unique Marks in Blogging Jessica Benton, ThatTechChick.com Darryl Frierson, Ashy2Classy.net Moderated By: Danyelle Little, TheCubicleChick.com 4:25 pm – 4:40 pm: SPONSOR TALK 4:40 pm – 5:00 pm: Reggie Levine, ReggieLevine.net ―Blogging On a Budget: Tips for Tech & Other Product Based Bloggers 5:00 pm – 5:30pm Wrap Up & Mix & Mingle Roshini Cope, Glamazini.com, Speaker, ―The Ten Commandments of a Successful YouTube Channel‖ Roshini is a glamorous amazing amazon (6 foot 1 inch) natural hair blogger & vlogger who has documented her journey on Glamazini.com (previously Roshini.net) for the past 8 years. She blogs about hair, products, tools, experiences, trials, errors, recoveries, other natural hair wearers, makeup & day-to- day life. Her passion is to tell her story while encouraging others toward ―life, liberty & the pursuit of nappyness‖. Visit her often at Glamazini.com, YouTube.com/Glamazini, & guest posting on blogs such as TheCubicleChick.com. Follow her at @Glamazini. Stef Pollack, Cupcake Project.com , Panelist, ―It's Not Just About the Food! Social Media, Food Blogging, & Broadening the ‗Menu' Online‖ Before Stefani Pollack began blogging in 2007, she had never baked a cupcake. Now, not only does her cupcake blog, Cupcake Project, have an average of 200,000 page views a month from readers coming to see her new recipes, but Stefani also develops original cupcakes monthly for Paula Deen. Stefani's blog has been featured in People Magazine & is a Saveur Magazine featured site. Stefani is also a member of the TLC Cake Crew, a select group of TLC's favorite baking bloggers. Locally, Stefani has been showcased on Fox 2 News & Show Me St. Louis. Visit Cupcake Project at www.cupcakeproject.com & follow Stefani on Twitter at @cupcakeproject and on Facebook at www.facebook.com/cupcakeproject. Jeff McIntire-Strasburg, Sustainablog.org, Speaker, ―Green Blogging For Fun & For Profit‖ Jeff McIntire-Strasburg is the founder & editor of sustainablog, a long-running environmental blog based in St. Louis. He's a former writer for Treehugger, a co-founder of Green Options Media (now Important Media), a current writer for the Sundance Channel's SUNfiltered blog, & a frequent contributor to many sites within the ―green blogosphere.‖ He's an adjunct instructor with Webster University's Professional Writing certificate program. Twitter: @sustainablog Facebook: http://www.facebook.com/jeff.mcintirestrasburg Stumbleupon: http://sustainablogger.stumbleupon.com Google Profile: http://www.google.com/profiles/sustainablog Vernon Ross, Blogger, Marketing Consultant, Ross Public Relations.com, Speaker, ―Gaining Sponsorship For Your Blog, Brand, Or Cause‖ Vernon teaches businesses how to earn sponsorship for their websites & brands. Vernon also speaks & writes about social media & how to incorporate it into your current marketing & promotion strategy. He has worked with Mc Donald's to promote their Midnight Gaming Championship & Rockstar games to conduct customer feedback tournaments. In addition he has also worked on promotional projects for New Line Home Cinema, Dub Magazine & EA Sports respectively. Web: http://rosspublicrelations.com Blog: http://blog.rosspublicrelations.com Twitter: @rosspr Abby Schwarz, Yelp.com St. Louis Community Manager, Panelist, ―It's Not Just About the Food! Social Media, Food Blogging, & Broadening the ‗Menu' Online‖ Abby Schwarz is the Community Manager for Yelp St. Louis. Yelp is a website where the smart & fabulous go to connect with local businesses. On the site, people read & write reviews about their favorite hair salons, restaurants, mechanics, etc. As of June 2010, over 32 million individuals visited the site & more than 11 million reviews have been written on Yelp. As the Community Manager, Abby's job is to nurture & grow the existing community through marketing & event planning. Catch her on Twitter- @yelpstlouis Reggie Levine, Reggie Levine.net, Speaker, ―Blogging on a Budget: Tips For Tech & Other Product Based Bloggers‖ Reggie Levine's love for technology began early in his life. For him, technology had always been a very personal experience until he found video blogging in 2007. (He still refers to himself as a Personal Technologist) Now he is a technology & video blogger who focuses on everyday technology for everyday folks. He reviews products, gives opinions & offers tips & tricks to make their experiences better. You can find his work at www.ReggieLevine.net & his video work at youtube.com/LeReg. Follow him on Twitter at twitter.com/LeReg Jim Barnthouse, Habanero, Speaker, ―Blog Branding: Use Your Style & Voice to Lure Readers & Keep Them!‖ Only a year & a half into moving from Chicago to St Louis, Jim Barnthouse has very quickly become a fixture in the local blogging community. Establishing his bloggy cred, Jim works as the Director of Social Media & Digital Strategy at the digital marketing agency, Habanero, where he can often be found giving high fives & complaining when thunderstorms don't live up to their promise. Sarcasm, humor & the occasional dirty word are the backbone of most of his musings on being a newcomer to St Louis on his popular St Louis-centric blog, www.ArchObserver.com. Even when disappointed in his discoveries (St Louis style pizza), his newfound love for St Louis keeps him investigating & embracing what make this city unique. You can find Jim regularly tweeting as @HabaneroWeb, @JimBarnthouse & the daily Arch Observer Facebook feed at www.Facebook.com/ArchObserver. Fun Fact: Jim plays for the St Louis Men's Roller Derby Team, The St Louis Gatekeepers. Melissa Gilliam, Marketing Director, YMCA Trout Lodge & Camp Lakewood, Speaker, ―Non-Profits: The Utilization & Implementation of Social Media & Social Networking To Spread Message‖ Melissa is a non-profit marketing professional whose career has spanned several cities including New York City, Pittsburgh, & St. Louis as well as Taipei, Taiwan. Melissa has worked in botanic, visual arts & performing arts institutions. She is currently the Marketing Director for YMCA Trout Lodge & Camp Lakewood in Potosi, Missouri where she manages all marketing & social media strategy & implementation for the institution. Melissa holds a Masters degree in Arts Management from Carnegie Mellon University. She is active with the Young Nonprofit Professionals of St. Louis, the Social Media Club of St. Louis, the Washington County Tourism Committee, & the Center of Creative Arts (COCA) Junior Board. Melissa's writing has been published by Americans for the Arts, Aire Magazine, River Hills Traveler & on iluvlocalplaces.com. Her first story is scheduled to publish in Insider 573 Magazine later this fall. Her blog, www.smalltowngirlsguide.com has been recognized by wordpress.com, & her photography has been republished by the BBC & Schmap.com. Melissa loves to bake & hike, & is currently training for her first half marathon. Robyn Wright, Robyn's Online World.com, Panelist, ―Mommy Blogging: From Bottles to Blogging to Business‖ Robyn is a stay-at-home-mom turned blogger. Since 2008 she has been sharing her real life world of being the mom to a teenage son, wife, foodie, geek, reviewer, & more on her blog, Robyn's Online World. During the past two years Robyn has established herself among the ―mommy bloggers‖ with a strong social media influence. She is active on her blog, Twitter, Facebook, Whrrl, LinkedIn, & YouTube & is always on the lookout for new social media outlets. Robyn was ranked as one of the Top 50 Power Moms by Nielsen & has been an ambassador with such brands as Kellogg's, Kleenex, & Tropicana. Robyn is also consistently among the Twitter Elite in St. Louis according to Grader.com. You can catch up with Robyn at RobynsOnlineWorld.com and follow her on Twitter @RobynsWorld. Darryl Frierson, Blogger, Writer, Promoter, Ashy2Classy.net, Panelist, ―Blogs That Break the Mold‖ Darryl Frierson is a graduate of Iowa State University with a degree in Journalism & Mass Communications & a degree in History. Born in Chicago & raised in St. Louis he has lived or traveled to almost every state in the United States. He describes himself as a Writer, Progressive Philosopher, Sports Junkie, & Promoter who Grinds hard like a 808. He is also working on his first book a romance comedy called ―Loose Ends‖. His Blog Ashy to Classy(www.ashy2classy.net) tackles everything from love an relationships, hip hop & life, to politics & social issues. He can be contacted on twitter(www,twitter.com/diggame) & his facebook fan page or Send me an email email@example.com Robert Little, Blogger & CEO of Bona Fide Media Group, BlackSportsOnline.com, ―How To Take Your Blog From The ‗Underground' to the ‗Mainstream'‖ Robert Littal (the Bona Fide Sports Expert) is the founder & CEO of BlackSportsOnline. Mr. Littal's vision of creating an outlet for minority writers to have a voice in the ever growing sports media has flourished to the point where BlackSportsOnline now employs multiple writers expressing views that are innovative, informative, controversial & most importantly unbiased. BlackSportsOnline has garnered multiple mentions on ESPN SportsCenter, Yahoo.com, Fanhouse.com, ESPN.com, Profootballtalk.com, TMZ.com, CNNSI.com, Deadspin.com, TheBigLead.com, SOHH.com, NecoleBitchie.com, MediaTakeOut.com, Maxboxing.com, Boxingscene.com, Sportsline.com among other sites & publications for our groundbreaking & refreshing way of reporting sports. Under the Bonafide Media Group, Mr. Littal now sports an impressive resume that includes working with the likes of TMZ Sports, The Washington Post & J'Adore Magazine. Mr. Littal has also started two successful sister sites: Breaklamps.com and MySpaceModelsonline.com. With over 5200 Twitter followers, 700+ Facebook Fans & 500,000 monthly readers Mr. Littal has positioned himself to be a power player in new media as we start a new decade. Linda Doty, JustLinda.net, Speaker, ―Laughing Out Loud (LOL): Social Media & the Comedy Within‖ Linda Doty has been blogging since 2005. She blogs about everything & anything: her job as an astronaut (more commonly known as a middle manager in Corporate America), her love for Red Lobster Cheddar Bay Biscuits, her 5 daughters who range in age from 6 to 27 (yes, in fact, she does know what causes that now), her 26 years of married life (not all to the same man, mind you, but still, she wants credit for all time-served!) She blogs at JustLinda.net & is currently a contributor for STLFamilyLife.com. In her spare time… oh, wait – she doesn't have any of that. Never mind! You can also follow her on Twitter at @JustLindaSTL. Danyelle Little, Blogger & Freelance Writer, TheCubicleChick.com, Moderator, ―Blogs That Break the Mold‖ Danyelle Little, aka ‗The Cubicle Chick', is a blogger, freelance writer, & author from St. Louis, Missouri. On her highly lauded blog, TheCubicleChick.com, she discusses a plethora of topics from parenting to health & beauty to pop culture & current events. Danyelle has managed to leave the cubicle behind, & is now a WAHM (working at home mom) doting on her two children. Danyelle has also become somewhat of a social media & networking fiend managing several accounts such as her Twitter & Facebook accounts, & she also works behind the scenes on other social media brands as a consultant. In addition, she writes for YBP Guide as a Career Columnist & has been featured on CNN.com, STLToday.com, & the websites The Fresh Xpress, Centric TV, & Fat Wallet.com. Not only is Danyelle a blogger & social networking mover & shaker, but she also founded Show Me the Blog Social Media & Blogging Conference, in hopes that it will be a twice-annual event held here in St. Louis. Follow her on Twitter @TheCubicleChick. Chris Reimer, Chief Marketing Officer, Scorch Agency, Panelist ―Show Me the Blog (And the Money!) The Real Jerry McGuire's of St. Louis Social Networking‖ Chris Reimer (@RizzoTees) traveled a somewhat circuitous path to arrive at the shores of Social Media. He graduated from Marquette University in 1993 with a B.S. in Accounting, became a CPA in 1995, & then served as Chief Financial Officer of several organizations around St. Louis, including Zipatoni. The entrepreneurial bug bit in October 2007, & the idea for Rizzo Tees was hatched. After a year of development, Rizzo Tees went live in October 2008. Five days later, Chris started marketing his tees (and himself) on Twitter. A Facebook fan page soon followed, then a Posterous blog, & then a small business & social media blog called The Basement Entrepreneur. All of this marketing activity led to his first real marketing job – Chief Marketing Officer of Scorch Agency. The shackles of accounting had been cast off! As Founder & ―Chief Shirtologist,‖ Chris was (and still is) responsible for marketing Rizzo Tees & growing it with no marketing budget. His Twitter account @RizzoTees is often ranked at or near #1 in St. Louis on both twitter.grader.com & wefollow.com. He was recently named the Most Influential St. Louis Tweep by STLTweets.com for the first half of 2010, beating out over 220,000 local Twitter users. Twitter remains his #1 sales channel, & Facebook is #2. His Twitter community continues to grow & is now over 40,000 followers. Chris sits on the Board of the Social Media Club of St. Louis & enjoys speaking to groups about social media & the impact it has had on his life. When he isn't spinning the yarn on Twitter, he sips a glass or two of red wine & watches any sport in hi-def. Most of all, he enjoys playing with his two daughters – nothing is better than chasing them around the playground at Francis Park. Angela Ortmann, STLWineGirl.com, Panelist, ―It's Not Just About the Food! Social Media, Food Blogging, & Broadening the ‗Menu' Online‖ STLwinegirl Angela Ortmann gained a passion for all things epicurean through her years of experience in luxury restaurants & hotels. A St. Louis native, Angela spent four years in San Francisco expanding her food & wine knowledge. She has worked in some of the top restaurants in the country & has trained at wineries, creameries & organic farms. Angela is not only a Certified Sommelier, but also a connoisseur of beer, spirits, cigars & cheese. One of her true loves lies in the pairing of food & wine. This enthusiasm led to a blog in 2008 which then led to the launch of her event & consultation business in 2009. STLwinegirl focuses on the enjoyment of the dining experience, whether it be at a five-star restaurant or in the comfort of your own home. By removing the element of intimidation, STLwinegirl is committed to making the world of food & wine approachable & more accessible. Angela hosts public classes & events as well as private events such as her highly sought-after In Home Tasting. Starting a business on her own with little to no capital was the driving force behind her involvement in social media & the opportunities there to connect with consumers on a personal & professional level. Angela has always felt strongly that her clients know her as more than just a business. Through her posts on Twitter (@STLwinegirl) & Facebook (www.facebook.com/STLwinegirl), followers can get a sense of Angela's real life as well as keep up to date on her events. Her favorite thing to share is what pairings she is enjoying at home. Angela is also a columnist for FEAST Magazine (www.feastSTL.com). Her monthly print column, ―On the Shelf,‖ showcases three new wine buys complete with tasting notes & pairing suggestions. Through her weekly online-exclusive column, ―Quite the Pair,‖ Angela dines around town, tasting dishes & offering pairing recommendations from the restaurant's own wine list. She also hosts monthly wine tastings for the publication. Heather Vaughn, WistfulWrists.com, Panelist, ―Mommy Blogging: From Bottles to Blogging to Business‖ Seven years ago, Heather left a career in journalism to become chief operating officer for her family, which would eventually grow to include four children. With a background in communications, public relations & video editing, Heather dove into social media to grow with emerging communication trends. Among her many other talents, Heather is a social media & tech consultant as well as a blogger. Heather blogs about crafts, parenting, life in a large family & technology at WistfulWrists.com. Since beginning her blog in 2009, Heather has participated in events & campaigns with brands such as JumpStart, Hallmark, Energizer, Kodak & most recently, Martha Stewart Living Omnimedia. You can follow her on Twitter @WistfulWrists. Wilma Jean, WilmaJean.com, Moderator, ―It's Not Just About the Food! Social Media, Food Blogging, & Broadening the ‗Menu' Online‖ Wilma, a freelance acrylic artist, has been making things for as long as she can remember. Her work has been featured in Empowerment, Boutique Online, & is on display locally around Atlanta. She also writes for Examiner.com. She has been blogging about her art, craft, writing, & other creativity for only a few years. Visit her blog at WilmaJeansBlog.blogspot.com & follow her @mslejean. Marijean Jaggers, V.P. , Standing Partnership, Speaker, ―Ambassador You: Integrating Social Media into Business Communications‖ As a vice president & manager of St. Louis-based reputation management firm Standing Partnership's satellite office in Charlottesville, Va., Marijean Jaggers is a social media specialist providing education & counsel to clients in several industries in the U.S. & Europe. She also provides counsel to clients throughout Central Virginia including the executive management team of the Charlottesville Albemarle Airport, managing the organization's media efforts. Marijean serves as the social media correspondent for The Newsplex, a group of television stations that are Central Virginia affiliates of ABC, CBS & Fox networks, providing insight & commentary on news stories related to digital communications. Marijean has been blogging as STLWorkingMom since early 2005, is the author of Change the Conversation, a blog about social media & how to use it, & is a frequent contributor & manager of Where Do You Stand?, the corporate blog of Standing Partnership. In 2004, Marijean was one of the first bloggers for the St. Louis Post-Dispatch, contributing the Shop Talk blog to the newspaper's online presence for a number of years. Marijean has spoken to numerous regional groups about using social media & social networks for business, career development & personal branding. She has provided workshops & presentations for the Charlottesville Chamber of Commerce, Quadruplicity, C'ville SheBlogs, Social Media Club Charlottesville, ActionCoach, Waynesboro Connect to Success business networking group, the Center for Nonprofit Excellence, Charlottesville Business Innovation Council & the Business Women's Round Table. With a strong commitment to community, Marijean serves as a board member of the Charlottesville Albemarle Technical Education Center (CATEC) Foundation & the Legal Aid Justice Center. She has also devoted time & effort as a board member of the American Diabetes Association & Delmar Magazine, an independent literary journal. She is a graduate of Lindenwood University where she earned a Bachelor of Arts in English. & in her spare time, Marijean bakes pies competitively. StandingPR.com, blog— MariJeanJaggers.com & STLWorkingMom.com, Twitter @marijean Melody Meiners, STLFamilyLife.com, Panelist, ―Mommy Blogging: From Bottles to Blogging to Business‖ Melody Meiners is a freelance writer & interactive marketing consultant who lives in St. Louis with her husband, drama-queen baby, & neurotic dog. She is learning that parenting changes everything, but she refuses to abandon her heels & martinis for the standard issue mom jeans & feathered haircut. She graduated from Webster University in 2005 with a degree in English Literature, & she is working feverishly to prove that she is qualified for something other than correcting other people's grammar while serving them fancy coffee drinks. She is the founder & managing editor of Girls Guide to the Galaxy, a partner & contributor at STLFamilyLife.com, & you can find her personal blog at MrsSmartyPants.com. And, of course, when she isn't blogging she is feverishly tweeting as CosmosGirl on Twitter. She is also the founder of St. Louis Women in Media, a local women's networking group for media professionals & hobbyists. Basically, she is a typical thirty-something working wife & mother who is desperately warding off those evil mom jean spirits & trying to make sure her kid doesn't grow up to shoot laser beams out of her eyes (a very likely scenario at this point). Jessica Benton, ThatTechChick.com/IDesign, Panelist, ―Blogs That Break the Mold‖ & Moderator, ―Mommy Blogging: From Bottles to Blogging to Business‖ Jessica Benton is a successful blogger & graphic designer. Her blog, That Tech Chick, is highly frequented by a diverse demographic of visitors. Jessica's designs are featured on a vast array of websites around the Internet & have even been offered in print media for her clients as well. Jessica has grown in experience & knowledge of social media & blogging throughout her decade-long involvement in online communities, engaging thousands of readers each week who compile an international following across her personal network. Jessica has a 15-year work resume in business management, where she has focused her skills in the areas of management, PR, training, & quality control. Jessica launched iDesign, a web, blog & print design company, in March 2010, after being laid off. Through iDesign, Jessica specializes in the transfer of blogs from various platforms into the WordPress blogging system, blog & graphic design, print graphics, media kits, & blog consultation as well as social media management & education. She has a passion for sharing & assisting with regards to her areas of expertise & regularly provides tech tips, tutorials, & information to those in search of guidance. After venturing into blogging in different niches, she found herself still answering the same tech questions all her family & friends asked, by her online connections. In an effort to shine with her love for technology, helping others, & being online, That Tech Chick emerged. Jessica wanted a place online where she could be the girlie girl she is & also share her love for smartphone apps, blogging tips, networking, & keep it all down to earth & easy for the average person to understand. All too often you find girly blogs. You also find many tech blogs. But how many girly tech blogs to you come across each day? Jessica also writes for the Beaumont Social Media Club & recently ventured into a co-blog with Danyelle Little named The Blogging Chicks, where together they blog about travel, opinions, & their networking escapades. Follow her on Tiwtter: @jess_benton Troika Brodsky, Communications Director, Schalfly Beer, Panelist, ―It's Not Just About the Food! Social Media, Food Blogging, & Broadening the ‗Menu' Online‖ Troika Brodsky is the Communications Director for St. Louis based craft brewery, Schalfly Beer. Founded in 1991, Schlafly Beer has grown to become the 41st largest craft brewery in the nation, the largest locally owned brewery in the St.Louis & the best selling craft beer in the city. The Schlafly Tap Room in downtown St.Louis & Schlafly Bottleworks in Maplewood, MO are home to two restaurants & combine to brew over 50 unique beer styles each year. Troika began at Schlafly in 2002 doing graphic design & video but his arm was often twisted into to helping out with beer tastings & beer festivals. As Communications Director for Schlafly Beer, Troika is the voice of Schlafly Beer on Facebook & Twitter & manages the brewery's online & mobile platforms, newsletters & media communications. He is still often forced to help out with beer tastings & beer festivals. Web: Schlafly.com Facebook: www.facebook.com/Schlafly.Beer.Fans Twitter: @Schlafly Stefany Thode, ToBeThode.com, Panelist, Mommy Blogging: From Bottles to Blogging to Business‖ Stefany Thode is a registered nurse turned stay-at-home mom. She started blogging in 2008 as a way to communicate the frustrations of being a stepmother to three and expectant mother. Two years and two children later, Stefany still finds solace in her blogging and has developed a loyal following of people willing to read about the chaos of her life. As a mom of five, Stefany talks about her kids often but you may also find posts about her pets (or ―mini-zoo‖), her life as a full-time student, her goals to become a Nurse Midwife, photography, cooking, or product reviews. Stefany has worked with companies such as Soft Scrub, VTech, LeapFrog, Clorox, Zhu Zhu Pets, and Warner Brothers to name a few. While once apprehensive of social media, Stefany is now active daily on Twitter as ToBeThode or on Facebook. Self- taught on all aspects of html and blogging, she enjoys sharing her knowledge with others and has been known to ―lend a hand‖ to bloggers in need. You can find Stefany at her blog, ToBeThode. *Please support our volunteer speakers by visiting their sites and following them on their social media networks *Please visit the sponsors' sites by clicking on their logo. Founder & Organizer- Danyelle Little, TheCubicleChick.com Communications Director- Yolonda Spinks, NotoriousSpinksTalks.com & BooksAND.com Conference & Publications Coordinator- Jessica Benton, ThatTechChick.com & iDesign Conference Photographer- Vivica Shade Graphic Designer- Kat Robertson, eDesign Pro Conference Chair- Robyn Wright, RobynsWorldOnline.com Volunteers- Danielle N. Lee & Robert Littal My family, most important, my husband for supporting me through this endeavor and being patient when I spent all of my time organizing the conference. To Jessica Benton, my partner and friend who has been most awesome. Without her, this conference would not have been possible. Robyn Wright, who encouraged me when the going was rough. Thanks so much! Your friendship means a lot to me. To Danielle Lee who stepped in at the last minute, THANK YOU! To all the speakers, panelists, and moderators who lent their time to my "little" conference. You guys amaze me! Attendees, you are what Show Me the Blog is all about! Your spirit and creativity keeps me on my toes, and I have learned from each and every one of you! Danyelle Little Show Me The Blog
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PASCAGOULA — Ingalls Shipbuilding in Pascagoula has authenticated the keel of the sixth U.S. Coast Guard national security cutter, Munro (WMSL 755). The ship is named to honor Coast Guard Signalman First Class Douglas A. Munro. Munro died on Sept. 27, 1942, on Guadalcanal while rescuing a detachment of Marines. For his actions, Munro was posthumously awarded the Medal of ...Read More »
Tag Archives: National Security Cutter
Ingalls to celebrate completion of Coast Guard cutter
PASCAGOULA — Ingalls Shipbuilding will deliver its fourth national security cutter to the Coast Guard tomorrow. The 10 a.m. ceremony for the cutter Hamilton will take place aboard the vessel. Participants will include Ingalls Shipbuilding President Brian Cuccias, Coast Guard Assistant Commandant Rear Adm. Bruce Baffer and Capt. Douglas Fears, the Hamilton's prospective commanding officer. Shipbuilders and crew members of ...Read More »
Senate committee okays funding for Coast Guard cutter
PASCAGOULA — The Senate Appropriations Committee has approved full funding for production of the U.S. Coast Guard's eighth National Security Cutter (NSC-8) at the Ingalls Shipbuilding in Pascagoula. U.S. Sen. Thad Cochran (R-Miss.) yesterday provided the update following the award of a $76.5-million long-lead materials contract for Ingalls to prepare for production of NSC-8. The Senate Appropriations Committee last week approved ...Read More »
Ingalls awarded $497M contract to build another cutter
PASCAGOULA – U.S. Sen. Thad Cochran (R-Miss.) reports a $497-million military shipbuilding contract award to Ingalls Shipyard in Pascagoula to construct the seventh national security cutter (NSC 7) for the U.S. Coast Guard. Cochran said the Department of Homeland Security is amending an existing contract with Ingalls to provide FY2014 appropriations to construct NSC 7. Cochran serves on the Senate ...Read More »
Delegation praises $487 million Pascagoula contract to build Coast Guard cutter
WASHINGTON – Sen. Thad Cochran, Sen. Roger Wicker and Rep. Steven Palazzo are praising the award of a $487 million contract that will allow shipyard workers in Mississippi to provide the U.S. Coast Guard with its sixth National Security Cutter (NSC). The U.S. Coast Guard has awarded the "fixed-price incentive firm target contract" to Huntington Ingalls Industries for the NSC ...Read More »


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Tag Archive for "Sedona accommodations"
El Portal Sedona has Two New Hotel Specials!
At El Portal Sedona we are offering two new Stay and See Packages! Stay at El Portal and See Sedona with your choice of hotel special… Hotel Special Package #1 Stay 3 nights in a deluxe room, experience a 2 hour jeep tour for two from Safari Adventure Tours, and a guided, full day Grand Canyon Tour all for ...
El Portal Sedona Hotel ~ The Beginning
El Portal Sedona Hotel was a long time dream of owners Steve and Connie Segner!
41st Annual Fiesta del Tlaquepaque in Sedona
On Saturday, September 13 Tlaquepaque Arts and Crafts Village will celebrate it's 41st Fiesta del Tlaquepaque - a celebration of Mexican Independence Day!
Sedona Day Trips – You'll Want More than Just One Day!
Sedona, Arizona is located near a multitude of locations that make perfect day trips! Owner, Steve Segner and staff offer guests personalized concierge services to help plan what guests want to do in and around Sedona, what they want to get out of their trip to Sedona!
Sedona Red Rocks Festival 2014
Something great is coming to Sedona next weekend - the 2014 Red Rocks Festival! And this year's performers are in a class of their own!
El Portal Sedona Hotel Announces New Last Minute July Special!
Take a trip to Sedona and get this great last minute Sedona Hotel special at El Portal!
El Portal Sedona Hotel's The Adobe Guest Room
Enjoy this "tour" of our Adobe Room - fully handicapped accessible, beautiful, a serene Sedona hotel room and that is pet friendly!
49th Annual Jerome Historic Home and Building Tour
The Annual Jerome Historic Home and Building Tour is the longest running home tour in Arizona! This year will include the newly renovated...
Sedona Destination Weddings
El Portal Sedona Hotel is an enchanting place for an exquisite wedding!
Sedona International Film Festival
Visit Sedona, Arizona and celebrate the 20th year of the Sedona International Film Festival!!!


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You can now invite anyone to meet at your favorite Starbucks.
Limited-time feature
46 year old woman | Nicholasville, KY, USA | Seeking men 35-50 within 30 miles
hope there is a good match out there let's meet and have fun
i am attractive and outgoing. i am sociable very affectionate and considerate and am looking for an attractive male of the same character to have fun with and to possibly build a lasting relationship with because i don't like the bar seen and am not sure where to look any more. my match would be attractive, considerate, honest, well built, financially, Mentally and Emotionally stable and a well rounded person who loves . motorcyle or a boat would be a total bonus.
Camping , Cooking , Dining out , Movies/Videos , Music and concerts , Nightclubs/Dancing , Playing cards , Travel/Sightseeing , Watching sports
Basketball, Football, Walking / Hiking
I exercise 1-2 times per week.
I like Dogs, Horses
Middle of the Road
love carribean, florida, tennessee big sun and water baby. love to dance. want to go to hawaii. love to go out to dinner. like a good steak or seafood resteraunt
love motorcycles and boats and that atmosphere and to take weekend trips. when it's raining out, stay in veg out and pile up. love to watch two and half men on monday nights. more of jeans kind of girl but like to dress up occasionally. love uk
love boating and motorcycles. love to go to dinner and movie and love life. very upbeat like to go to comedy club or to movies or just stay in and cook and watch movie
dannille steel
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
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|5'10" (177cms) to 6'10" (208cms)
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text:
AT&T claims that because Google blocks certain numbers from association with its Google Voice service, it violates net neutrality principles. [NYT] It's all about fees at the back end: poky local telcos scam the big carriers on connection charges. But whereas big carriers are forced to allow the connections, Google Voice is not. The flaw in the argument is, of course, that Google Voice isn't a telco. It's a new application of existing technology that supercedes the business model that telcos rely on. Among other things, Google Voice makes it obvious that the services carriers charge for are worthless, and that bandwidth is their only real product.
AT&T complains about Google Voice
• Discuss this post in our forums
3 Responses to "AT&T complains about Google Voice"
Unfortunately, if we want to reign the telcos in with explicitly defined laws, we need to be prepared to accept the setback if the letter of the law inadvertently catches a few dolphins in the net. I think this is an unfortunate but fair claim to make against Google.
Voice should be treated just like any other data once it's entered the network It goes where the customer wants it to go.
Google should have every right, however, to defer any excessive costs of providing this service onto the user, provided the use is the user's choice.
This is a case where Google does actually have a real, prohibitive expense of providing the service that it cannot control. It isn't like a telco or ISP making claims of limited bandwidth because they're too cheap to upgrade their infrastructure. Google should have to provide connectivity without discrimination, but should get to charge customers for the privilege as a means of either discouraging the expense or being recompensed for it.
*slow clap @ #1*
"Among other things, Google Voice makes it obvious that the services carriers charge for are worthless, and that bandwidth is their only real product."


text:
Fast and Furious: Solo parti originali [BDRip 720p Ita Eng Ac3 DTS - SUB] [Tntvillage]
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Added on Dec 4, 2010 in Movies > Dubbed Movies
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Cast: Vin Diesel, Laz Alonso, Lou Beatty Jr., Jordana Brewster, Neil Brown Jr., Wilmer Calderon, Greg Collins, Jack Conley, Mikal Delaney, Roger Fan and others
Brian O'Conner, now working for the FBI in LA, teams up with Dominic Toretto to bring down a heroin importer by infiltrating his operation.
Written by Chev.Chelios317.01K
Fast and Furious: Solo parti originali [BDRip 720p Ita Eng Ac3 DTS - SUB] [Tntvillage] (Size: 4.65 GB)
Name: Fast and Furious: Solo Parti Originali / Fast and Furious (2009)
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