text:
List of bugs that are fixed in BizTalk Server 2004 Service Pack 2
Article ID: 924330 - View products that this article applies to.
This article has been archived. It is offered "as is" and will no longer be updated.
On This Page
This article is a listing of article numbers for bugs that were fixed in BizTalk Server 2004 Service Pack 2 (SP2). Click the article number that comes before the title of the bug to view the article.
For more information about how to obtain the latest service pack for BizTalk Server 2004, click the following article number to view the article in the Microsoft Knowledge Base:
(http://support.microsoft.com/kb/888751/ )How to obtain the latest BizTalk Server 2004 service pack
Service Pack 2
(http://support.microsoft.com/kb/838963/ )FIX: Only the first FTP command runs when you specify multiple FTP commands in the "After Put" property of an FTP send handler in BizTalk Server 2004
(http://support.microsoft.com/kb/884062/ )A System.OutOfMemory exception occurs when you compile a solution map that has a complex source schema or a complex destination schema
(http://support.microsoft.com/kb/884582/ )The character of the segment terminator of a functional acknowledgement may be ignored in the Microsoft BizTalk Server 2004 EDI adapter
(http://support.microsoft.com/kb/886674/ )FIX: The signed message that you receive from BizTalk SharePoint Messaging Adapter cannot be verified in BizTalk Server 2004
(http://support.microsoft.com/kb/886966/ )FIX: Slow performance on startup when you process a high volume of messages through the SOAP adapter in BizTalk Server 2006 or in BizTalk Server 2004
(http://support.microsoft.com/kb/888309/ )FIX: The Value Extractor functoid may return a value that is not correct when BizTalk Server 2004 is under a heavy load
(http://support.microsoft.com/kb/888320/ )FIX: Some party aliases may not be created when you run the BizTalk Deployment Wizard in BizTalk Server 2004
(http://support.microsoft.com/kb/889077/ )FIX: Delivery notification in a send port causes performance degradation in BizTalk Server 2004
(http://support.microsoft.com/kb/889164/ )FIX: The edi_datatype property is set to "A" when the value should be "AN" in BizTalk Server 2004
(http://support.microsoft.com/kb/889322/ )FIX: The SQL Transport Schema Generation Wizard generates a schema that contains only a Success node in BizTalk Server 2004
(http://support.microsoft.com/kb/890174/ )FIX: The BizTalk Server 2004 HTTP receive adapter does not fully support IIS ISAPI authentication filters for incoming messages
(http://support.microsoft.com/kb/890241/ )FIX: A newer version of an assembly may unexpectedly stop working when you undeploy an older version of the assembly in BizTalk Server 2004
(http://support.microsoft.com/kb/890530/ )FIX: You receive a "The root element is missing" error message when you try to process a message by using a Message Queuing transport adapter send port in BizTalk Server 2004
(http://support.microsoft.com/kb/890731/ )FIX: The Btsntsvc.exe process uses a high amount of CPU resources and an orchestration never finishes in BizTalk Server 2004
(http://support.microsoft.com/kb/890754/ )FIX: SQL Server blocking may occur when you have the Message Body Tracking feature enabled in BizTalk Server 2004
(http://support.microsoft.com/kb/890769/ )BUG: The Base EDI adapter may generate incorrect XML for some schemas in BizTalk Server 2004
(http://support.microsoft.com/kb/890840/ )FIX: The Before Receive tracking feature may not capture the correct message in BizTalk Server 2004
(http://support.microsoft.com/kb/890842/ )FIX: You may experience suboptimal performance when you use Value Mapping functoids in BizTalk Server 2004
(http://support.microsoft.com/kb/891015/ )Event ID 5720 is logged in the Application log after you remove an assembly in BizTalk Server 2004
(http://support.microsoft.com/kb/891045/ )FIX: You receive an "error B1000" error message when you try to export an orchestration to a BPEL file when the orchestration contains a variable of type System.Xml.XmlDocument in BizTalk Server 2004
(http://support.microsoft.com/kb/891441/ )You may receive a "Document Locked" error message in the BizTalk Server 2004 error log when you save an InfoPath document directly to a Windows SharePoint Services site
(http://support.microsoft.com/kb/891447/ )FIX: A distinguished field returns incorrect content when you use the empty <FieldName /> element tag form for the empty field in BizTalk Server 2004
(http://support.microsoft.com/kb/892048/ )FIX: An orchestration in BizTalk Server 2004 is suspended and is marked as "not resumable" when the SQL Server-based cluster fails over
(http://support.microsoft.com/kb/892138/ )FIX: A message is discarded when you start a serial convoy orchestration after multiple messages are submitted in BizTalk Server 2004
(http://support.microsoft.com/kb/892405/ )FIX: Messages are processed and delivered even if the orchestration instance finishes with a status of "Completed with discarded messages" in BizTalk Server 2004
(http://support.microsoft.com/kb/892491/ )FIX: BizTalk Server 2004 performance may be poor and your CPU utilization may be high on the SQL Server-based server that hosts the BizTalk Server databases
(http://support.microsoft.com/kb/892921/ )You receive different HTTP status codes when you post a document to a one-way HTTP receive port in BizTalk Server 2004
(http://support.microsoft.com/kb/893287/ )FIX: The resulting XML instances do not contain the correct number of N1Loop occurrences after the BizTalk Server 2004 Base EDI adapter processes an EDI flat-file document
(http://support.microsoft.com/kb/893754/ )FIX: You experience an unexpected increase in memory usage when you deploy and enlist an orchestration in BizTalk Server 2004
(http://support.microsoft.com/kb/894060/ )FIX: BizTalk Server 2004 incorrectly generates repeated NACK messages for a BizTalk Framework message that remains in the suspended queue
(http://support.microsoft.com/kb/894177/ )FIX: You receive an "Exception Caught: Cannot use a prefix with an empty namespace" error message when you use a Transform shape to generate a map in BizTalk Server 2004 Service Pack 1
(http://support.microsoft.com/kb/894253/ )FIX: The dtasp_PruneTrackingdatabase() stored procedure may take many hours to clean up the DTA database in BizTalk Server 2004
(http://support.microsoft.com/kb/894683/ )FIX: The Base EDI Adapter does not process a large EDI message and Event ID 23 is logged in the Application log in BizTalk Server 2004
(http://support.microsoft.com/kb/894785/ )FIX: You receive a "Native serialization error: (The target document in this mapping is not recognized)" error message when you test a map that uses a valid 4030 format 810 schema in BizTalk Server 2004
(http://support.microsoft.com/kb/894792/ )FIX: The receive locations do not accept the newly submitted documents when you deploy a new version of a pipeline assembly in BizTalk Server 2004
(http://support.microsoft.com/kb/894919/ )FIX: You receive a "Timeout expired" error message when you try to compile a BizTalk Server 2004 project that contains an EDI schema in Visual Studio .NET 2003
(http://support.microsoft.com/kb/894920/ )FIX: The EDI X-12 856 Ship Confirmation (X12_4010_856) schema has only two HL segments in BizTalk Server 2004
(http://support.microsoft.com/kb/895107/ )The HTTP adapter incorrectly converts the whole ContentType header to lowercase characters in BizTalk Server 2004 Service Pack 1
(http://support.microsoft.com/kb/895175/ )FIX: An orchestration is not completed successfully, and an exception is logged in an atomic transaction in BizTalk Server 2004 Service Pack 1
(http://support.microsoft.com/kb/895264/ )FIX: You receive an error message when you call the GetCommonValue method against an empty element in a BizTalk Server map
(http://support.microsoft.com/kb/895955/ )FIX: Error message when you run the sp_GetRemoteServerName stored procedure in BizTalk Server 2004 Service Pack 1: "Msg 7403, Level 16, State 1"
(http://support.microsoft.com/kb/896080/ )FIX: Some messages that are picked up by using a File receive adapter location may be lost in BizTalk Server 2004
(http://support.microsoft.com/kb/896152/ )FIX: The exception handler in Orchestration is not triggered if you experience an error in your outbound map in BizTalk Server 2004
(http://support.microsoft.com/kb/896673/ )FIX: A 10-second delay occurs before a message is received by the file receive location in BizTalk Server 2004
(http://support.microsoft.com/kb/898106/ )FIX: Message processing may slow or stop after you install BizTalk Server 2004 Service Pack 1
(http://support.microsoft.com/kb/899654/ )FIX: You may receive a "btm1050: XSL transform error" error message when you test a map in BizTalk Server 2004
(http://support.microsoft.com/kb/900083/ )FIX: The tracking event stream is not exposed in BizTalk Server 2004 orchestrations
(http://support.microsoft.com/kb/900339/ )FIX: You may experience slow performance when you configure the HAT tool to save all tracked messages for an orchestration in BizTalk Server 2004
(http://support.microsoft.com/kb/900400/ )FIX: You may experience intermittent lock conditions when you have multiple BizTalkMsgBoxDb databases configured in BizTalk Server 2004
(http://support.microsoft.com/kb/900467/ )FIX: You experience deadlocks on the BizTalkMsgBox database after you install BizTalk Server 2004 Service Pack 1
(http://support.microsoft.com/kb/901028/ )FIX: Event ID 5719 may occur when you use a custom receive pipeline to validate a document structure in BizTalk Server 2004
(http://support.microsoft.com/kb/901186/ )FIX: Message processing may stop, you may receive an event ID error message, and the MSDTC service may stop committing transactions when you map a large document in BizTalk Server 2004
(http://support.microsoft.com/kb/902102/ )FIX: The BizTalk Server 2004 FTP send adapter may send duplicate messages when you configure two FTP send ports to use the same BTS.ReceivePortName filter
(http://support.microsoft.com/kb/902397/ )FIX: You receive a "SQL Server does not exist or access denied" error message when you try to view tracked policy execution events in the Health and Activity Tracking tool
(http://support.microsoft.com/kb/903874/ )FIX: An "Index was outside the bounds of the array" error message is logged and existing messages do not correctly correlate after you install BizTalk Server 2004 Service Pack 1
(http://support.microsoft.com/kb/904168/ )FIX: An "Enterprise Single Sign-On is offline" error message may be logged in the Configuration log when you try to use the "Configframework /s" command to silently reconfigure BizTalk Server 2004
(http://support.microsoft.com/kb/904844/ )FIX: Error message when a flat file parser parses a message that contains blank spaces in BizTalk Server 2004 Service Pack 1: "The element 'MessageUserReference_108' has incomplete content"
(http://support.microsoft.com/kb/904950/ )FIX: The performance of a singleton orchestration that contains a Call Orchestration shape may decrease over time in BizTalk Server 2004
(http://support.microsoft.com/kb/905290/ )FIX: The rule engine may return incorrect values in BizTalk Server 2004
(http://support.microsoft.com/kb/905571/ )FIX: The Business Rule Engine may not process rules that have different priorities when a server is under a heavy load in BizTalk Server 2004
(http://support.microsoft.com/kb/905830/ )FIX: The XLANG engine may not report a failed DTC transaction and may not end the transaction in BizTalk Server 2004
(http://support.microsoft.com/kb/907344/ )FIX: You may receive a "Failed to create project" error message when you try to publish an orchestration as a Web service in BizTalk Server 2004
(http://support.microsoft.com/kb/908023/ )FIX: Per-instance pipeline configuration does not work as expected in BizTalk Server 2004
(http://support.microsoft.com/kb/908157/ )FIX: You notice many dehydrated service instances when you run a query in BizTalk Server 2004 Health and Activity Tracking
(http://support.microsoft.com/kb/908257/ )FIX: Error message when you use the ISSOTicket.IssueTicket method in BizTalk Server 2004: "80070057: Invalid parameter"
(http://support.microsoft.com/kb/908359/ )FIX: The Business Activity Services Messaging Adapter does not work as expected in BizTalk Server 2004 after you install Windows SharePoint Services Service Pack 2
(http://support.microsoft.com/kb/909009/ )FIX: Error message when you try to save message bodies that are tracked by a PassThrough pipeline in BizTalk Server 2004: "Internal failure"
(http://support.microsoft.com/kb/909945/ )FIX: You receive out-of-memory exception error messages when an orchestration contains Delay shapes in BizTalk Server 2004
(http://support.microsoft.com/kb/910142/ )FIX: The BizTalk Service may appear to stop responding and you may receive out-of-memory error messages in BizTalk Server 2004
(http://support.microsoft.com/kb/910400/ )FIX: The bts_fnFindConvoySet function in BizTalk Server 2004 uses lots of SQL Server CPU resources
(http://support.microsoft.com/kb/910912/ )FIX: The generated file contains two message body parts when you create a custom pipeline that contains only a MIME Decoder in BizTalk Server 2004
(http://support.microsoft.com/kb/912031/ )FIX: You cannot deploy a BizTalk assembly, and you may receive an error message in BizTalk Server 2004
(http://support.microsoft.com/kb/912276/ )FIX: Performance may decrease over time when you use a long-running orchestration in BizTalk Server 2004
(http://support.microsoft.com/kb/912483/ )FIX: A property may not be promoted as expected when you use the BTF Disassembler pipeline component in a custom pipeline to process BTF documents in BizTalk Server 2004
(http://support.microsoft.com/kb/912504/ )FIX: After a failover of the SQL Server cluster resource, response messages are suspended in BizTalk Server 2004
(http://support.microsoft.com/kb/913669/ )FIX: The Base EDI adapter reads only the first AK2 segment in the 997 functional acknowledgement in BizTalk Server 2004
(http://support.microsoft.com/kb/914018/ )FIX: A memory leak may occur in the BizTalk service when a receive location uses the MSMQT adapter in BizTalk Server 2004
(http://support.microsoft.com/kb/914299/ )FIX: The memory may not be released as expected when you compile a BizTalk Server 2004 project
(http://support.microsoft.com/kb/915576/ )FIX: White spaces are removed from promoted fields after map execution in an orchestration after you install BizTalk Server 2004 S915576
(http://support.microsoft.com/kb/915600/ )FIX: You must manually configure the Log Shipping feature in BizTalk Server 2004 Service Pack 1
(http://support.microsoft.com/kb/917160/ )FIX: The SMTP adapter does not try to resend a BizTalk Framework document in BizTalk Server 2004
(http://support.microsoft.com/kb/917871/ )FIX: In BizTalk Server 2004, a signed MIME message may be marked as falsified when the message is received by an SMTP client
(http://support.microsoft.com/kb/918343/ )FIX: The MSMQT adapter may unexpectedly stop processing messages if you experience database failure or intermittent connectivity issues in BizTalk Server 2004
(http://support.microsoft.com/kb/918758/ )FIX: An orchestration may stop responding and the message status is "Delivered Not Consumed" in BizTalk Server 2004
(http://support.microsoft.com/kb/919229/ )FIX: The MSMQT receive adapter sends a BAD_DESTINATION_NACK negative acknowledgement after you manually fail over an MSMQT receive port in BizTalk Server 2004
(http://support.microsoft.com/kb/919230/ )FIX: Error message when the MessageBox_Message_ManageRefCountLog_BizTalkMsgBoxDb SQL Server job fails in BizTalk Server 2004: "Arithmetic overflow error"
(http://support.microsoft.com/kb/919339/ )FIX: Error message when you try to change the ADO.NET connection string of an SQL send port on a server that is running BizTalk Server 2004: "HRESULT: 0x80131500"
(http://support.microsoft.com/kb/920136/ )FIX: Some Business Rule Engine values in the Windows registry are reset to the default values in BizTalk Server 2004
(http://support.microsoft.com/kb/923056/ )FIX: Event ID: 5754 when you use the SOAP adapter or the HTTP adapter together with a client certificate in BizTalk Server 2004
(http://support.microsoft.com/kb/923531/ )FIX: Field contents may be truncated when you use the BizTalk Server 2004 Flat File Assembler to serialize and then send a SWIFT FIN message to SWIFT
(http://support.microsoft.com/kb/923534/ )You cannot configure the BizTalk host instance to use a Windows group name that exceeds 63 characters in BizTalk Server 2006 and in BizTalk Server 2004
(http://support.microsoft.com/kb/904949/ )FIX: The MessageID property in the Health and Activity Tracking (HAT) tool does not match the MessageID property of the corresponding output file when you enable the Message Body Tracking feature in BizTalk Server 2004
(http://support.microsoft.com/kb/911617/ )FIX: Side-by-side deployment does not work as expected when convoys are used in orchestrations in BizTalk Server 2004
Article ID: 924330 - Last Review: January 17, 2015 - Revision: 4.1


text:
F-22 Raptor
by Lonwlf4 (08/02/07) 94,186 views
Clip of F-22 Raptor demonstration at EAA Airventure 7-28-2007.
Clip of F-22 Raptor demonstration at EAA Airventure 7-28-2007.
The aircraft that changed warfare forever.
US new military fighters. 150 million dollars worth.
Cool Demonstration video of the best Aircraft in the world!
the worlds most craziest jet fighters
Aviation - Military - High Angle of Attack Maneuver
F-22 Raptor Demonstration F-22 Raptor Demo Team Major Paul "Max" Mo...
excellent pics of F-16 & F-22 with (linkinparksSsession)
F-22 Raptor take off
The original F-22 is a highly agile fighter aircraft, but what this...
Watch out amazing air refuelling of F-22 Raptor
At the California Capitol Airshow on 6/10/07
If you're an RC fanatic, don't forget to subscribe, since I am regu...
the F-22 one mad looking fighter jet
Russian jet fighter SU-37 and american F-22 are perfect enemies. Pe...
The F-22 airshow demo at the Sacramento Airshow was awesome! The p...
F-22 Raptor Demonstration Major Paul "Max" Moga USAF Heritage Flig...
<b>F-22</b> Raptor music video
flyfly model F-22 ROG
This is the third installment of a quick and dirty build tutorial o...
2014 was a great year -- so thanks for being a part of it!
Ownage Pranks called a guy who was selling his Acura TSX. He absolutely hates getting low offers for his car.
This is how you smash a plugged-in TV... in super-slow motion.
Here at Epic Meal Time, we take pizza seriously. So seriously, in fact, that we're looking to provide pizza with the beautiful life it deserves.
Furious Pete gives you some step-by-step instructions on how to enjoy In-N-Out.

subset: commoncrawl


text:
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Increase my visibility!{{else}} {{#if IsMultiPack}}
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You can now invite anyone to meet at your favorite Starbucks.
Limited-time feature
48 year old man | San Diego, CA, USA | Seeking women 36-50 within 25 miles
Active within 24 hours
I'm a career oriented professional. I enjoy my...
I'm a career oriented professional. I enjoy my independence but I'm also missing a special someone. I'm fit and balanced. I eat healthy, but I don't deprive myself of anything I like. I recently took up Salsa dancing, just for fun. I prefer to exercise than to watch other people doing it, my job is sedentary enough. I'm a Navy Vet, and traveled all over, but I still love going to places with the right company. While I'm not religious, I live by the Golden Rule, trying to be a man of value, not necessarily of success. Every day is a gift that should not be wasted. I read as much as I can and I prefer quality over quantity. Regarding my match, I'll be happy with a normal girl...normal good, that is.
Coffee and conversation , Dining out , Gardening/Landscaping , Hobbies and crafts , Museums and art , Music and concerts , Exploring new areas , Performing arts , Playing sports , Travel/Sightseeing , Wine tasting
Dancing, Running, Soccer, Swimming, Walking / Hiking, Weights / Machines
I exercise 3-4 times per week.
I don't believe in astrology
I like Dogs
Middle of the Road
A California Casual-Sophisticated or Spanish restaurant sampling tapas and red wine. Coffee shops, specially the ones with adjacent bookstores.
Your high heels, black coffee, red wine, dark chocolate, almonds and any dessert with liquor in it. Aside from the ones already mentioned above, KPBS Radio and a little bit of TV, shows like The Office and The Big Bang Theory etc...
Salsa, Classical/Flamenco guitar playing, dining out, good parties, spectacular views, and just about anything with a good company.
Blink, some Match profiles, technical literature for work and others for fun.
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What he is looking for
|5'6" (167cms)
|5'0" (152cms) to 5'7" (170cms)
|Body type:
|Athletic and toned
|Slender, About average, Curvy, Athletic and toned
|Black, Blue, Brown, Grey, Green, Hazel
|Light brown
|Auburn / Red, Black, Light brown, Dark brown, Blonde, Salt and pepper
|No Way
|No Way
|Social Drinker
|Social Drinker
|Technical / Science / Computers / Engineering
|No preference
Research and Development
|$100,001 to $150,000
|No preference
|Never Married, Widow / Widower, Divorced
|Have kids:
|Yes, and they live away from home (1)
|No preference
|Wants kids:
|Not sure
|No, but it's OK if my partner has kids
|Latino / Hispanic, White / Caucasian
|Asian, Black / African descent, East Indian, Latino / Hispanic, Middle Eastern, Native American, Pacific Islander, White / Caucasian
I'm of Spanish/Scottish-American ancestry. But I see myself as a world citizen. The differences between different cultures are very superficial.
|No preference
I understand and respect the importance of religion for some people. It is ok if you are religious.
|English, Spanish
|Graduate degree
|Bachelors degree, Graduate degree, PhD / Post Doctoral
Masters Degree in Electrical Engineering


text:
Having the gumption to risk failure in order to succeed is a notion that touches all professionals involved in important enterprises at one time or another in a career. Dealing with the frustration and continuing on past the urge to just toss a task or project 'in the can' is more than just a character builder, but an integral part to measurable, and unmeasurable success. Bob, Challenge Board member
Team: 59
School: Los Alamos Mid
Area of Science: Earth and Space Sciences
Interim: Interim Report
Is New Mexico's Climate Changing
By Lillian Petersen and Lily Houghteling
Definition of the Problem
In this project, we are going to ask questions about climate change using python. Some of the questions we will ask are, has the climate in New Mexico been getting warmer over the last 100 years, is there more or less precipitation as it gets warmer, is each month by itself getting warmer and drier, and how much more do we expect New Mexico to warm in the future.
Plan for Solving Problem
The plan is to get the weather station data we need to conduct the experiment. Then, write a computer program in python to import the data, perform calculations, and plot the results.
So far, we have downloaded the weather station data from NOAA, and loaded the information into python. We also viewed the data in an excel spreadsheet. We downloaded data from Los Alamos, Taos, Carlsbad, and Albuquerque.
Expected Results
We expect that the temperature has risen at a low rate and there will be a fluctuating exponential curve. We also expect that the precipitation has a high variability and is decreasing over all. We would also like to forecast of future climate change by extrapolating data.
Hello World! Computer Programming for Kids and Other Beginners, Warren D. Sande and Carter Sande, 2009, Manning, 432 pages.
Weather station data from http://www1.ncdc.noaa.gov
Climate Change: A Very Short Introduction by Mark Maslin December 1, 2014 Oxford University Press
Climate Change: Evidence and Causes, The National Academy of Sciences, 2014, National Academies Press
The Climate Crisis: An Introductory Guide to Climate Change, David Archer, 2010, Cambridge University Press
Team Members:
Lillian Petersen Lily Houghteling
Sponsoring Teacher: Mark Petersen
Mail the entire Team
Tweet #SupercomputingChallenge


text:
SIP Report: 163 Steroid Tests Requested
From the Thoroughbred Owners and Breeders Association
The data provided to TOBA by the three sales companies consists of the total number of horses tested for exogenous anabolic steroids with the corresponding test results and the number of submissions to the ownership registry with the corresponding number of ownership registry inquiries. The data is inclusive of Fasig-Tipton's fall yearling sale in Lexington.
The data indicates that from a total of 6,230 yearlings sold in nine sales, 163 were tested for exogenous anabolic steroids. Of the 163 horses tested, one positive test result was returned from the August OBS sale. The positive finding did not result in a return as the buyer maintained ownership of the horse.
Information was voluntarily submitted to the ownership registry for 1,257 horses, and there were two inquiries made to view the ownership registry. OBS does not offer an ownership registry.
"This data gives us an interesting but preliminary indication of how the market is responding to our reform measures," said D.G. Van Clief Jr., chairman of the Sales Integrity Monitoring Committee. "The good news is probably that activity levels don't indicate high levels of concern amongst participants. Nonetheless, we will continue to monitor the markets closely to insure that they are operating with the utmost integrity."
In 2008, OBS was the first sale company to begin testing for steroids at 2-year-old sales. OBS tested 53 horses out of the 1,558 2-year-olds sold this year. No positive test results were returned. Keeneland and Fasig-Tipton will begin testing for steroids at their 2009 2-year-old sales.
The testing of weanlings and yearlings for exogenous anabolic steroids and the establishment of the ownership registry were added as new conditions of sale by Fasig-Tipton and Keeneland as a result of the recommendations of the Sales Integrity Task Force in 2007. In addition, the task force created an enforceable bloodstock agent code of conduct and an updated list of prohibited practices.
TOBA launched a Web site for the Sales Integrity Program in July that further describes an auction code of ethics.
Copyright © 2015 The Blood-Horse, Inc. All Rights Reserved.


text:
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text:
Special Offer
Publisher's Description+
EyeLine Video Surveillance Features: Records multiple cameras simultaneously. Number limited only by hardware. Motion detection recording saves spaces by recording only when something is happening. The frames are automatically time stamped so it can be used as evidence if required. Video can be monitored on the screen as it records. Easy to use for reliable day-to-day operation.
What's new in this version:
Version 2.03 may include unspecified updates, enhancements, or bug fixes.


text:
Investors are making big, bearish options bets on high-yield corporate bonds ahead of an expected increase in interest rates from the Federal Reserve. Read More »
May 6, 2015
Investors are making big, bearish options bets on high-yield corporate bonds ahead of an expected increase in interest rates from the Federal Reserve. Read More »
Options traders are gearing up for Tesla's biggest earnings move in a year. Read More »
Options traders are betting on another sizeable move from Pandora Media Inc. after its first-quarter earnings, but that move is expected to fall short of recent swings. Read More »
A collision of four different flavors of options and futures contracts happens once a quarter, and tends to boost stock-market volumes as investors adjust positions. But the so-called "quadruple witching" can no longer be counted on to deliver increased volatility. Read More »
An options-market gauge is suggesting that protective put options on stock indexes have become pricey, but that doesn't mean investors are girding for an immediate pullback. Read More »
Options traders are betting that swings in Tesla shares could rev up after it reports fourth-quarter earnings after the bell Wednesday. Read More »
When Twitter Inc. reports earnings after the closing bell Thursday, options traders are betting on a smaller-than-usual move for the stock. Read More »
The options market is pricing in the smallest earnings move from Amazon.com Inc. in a year and a half. Read More »
Earnings from Netflix Inc. are likely to spark a sizable swing in the video-streaming company's stock, but that move is expected to be smaller than in some recent quarters. Read More »
Trading in options on the market's so-called fear gauge indicates that some investors are preparing for a few weeks of calm. Read More »
Content engaging our readers now, with additional prominence accorded if the story is rapidly gaining attention. Our WSJ algorithm comprises 30% page views, 20% Facebook, 20% Twitter, 20% email shares and 10% comments.


text:
Warm Brew
Song: Can Ya Blame Me
Album: Ghetto Beach Boyz
I don't know about y'all, but I like to start my morning off with a nice Warm Brew. OK, not really; I prefer my beer chilled and, even on thirsty Thursdays, I try to hold off on the liquor... More
Posted 6 months ago
Song: Whispers
Album: Ghetto Beach Boyz
More than a year after the release of the group's last project, The Ride, Warm Brew fans are likely feeling a little bit parched. Today, the Los Angeles threesome finally return with a little... More
Posted 7 months ago
Editor's Pick
Song: Proper Amount
Whether you're talking weed, liquor, cash or you-name-it, there's a Proper Amount for everything—and, seeing as the West Coast quartet haven't stepped into the Booth with a new... More
Posted 2 years ago
Song: Hear Ya Say
After their Search For Paradise back in May, Los Angeles-based trio Warm Brew have returned to our pages with the refreshingly optimistic Hear Ya Say. The single starts off with a familiar sample from... More
Posted 2 years ago
Song: Search for Paradise
Fresh off the release of their latest digital album, Warm Brew return to our pages with a standout off the project. On Searching for Paradise, Danny D's low-key, organ-driven production sets the... More
Posted 3 years ago
Song: Creep (Serk Spliff Mix)
Just in time for Valentine's Day, Los Angeles crew Warm Brew step into the Booth with their gender-switched rendition on TLC's 1994 hit single, Creep. Over Dallas Austin's... More
Posted 3 years ago
Song: Screen Play
While Warm Brew's highly-anticipated debut set still lacks a title and release date, the Los Angeles crew aren't about to let listeners go thirsty in the meantime. To tide fans over,... More
Posted 3 years ago
Los Angeles crew Warm Brew like to get inebriated as much as the next guys—I mean, you've got to be a partier to name yourself after room-temperature beer—but they do have their limits. The... More
Posted 3 years ago
Editor's Pick
Song: Nothin From Nothin
Despite their unappetizing stage name, the Warm Brew didn't fail to leave Booth readers refreshed when they introduced themselves with early-April's coffee-shop-funky Willing to Ride.... More
Posted 4 years ago
Editor's Pick
Song: Willing to Ride
Though Booth newcomers Warm Brew were evidently thinking of beer when they chose their collective stage name (judging by the crushed-can wallpaper on their Tumblr), their sound brings to mind the... More
Posted 4 years ago
Sample Text - Sample Link


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Fitness Marketing Expert Offers Tips for Starting a Personal Training Business
Bedros Keuilian, fitness marketing expert and creator of the popular blog PTPower.com, has just released a brand new video offering advice to fitness professionals looking start a personal training business on a budget. Keuilian admits that he made this video in order to help more personal trainers who lack a strong business background find the best methods for opening a facility without going into major debt.
He explains, "I wanted to make this video so I could reach fitness professionals and give them solid advice on starting a business because a lot of them tend to go the extra mile and spend more than they should when opening a training facility. I wanted to prevent a headache for personal trainers looking to venture out."
In this new video, Keuilian goes over important topics like how to choose a location, how to leave extra money on the table for critical business expenses such as marketing, and ways to find more personal training clients.
"Don't spend so much time and money on creating the perfect workout space. Make it look nice, but don't go to the extreme when designing your space and overspend," says Keuilian. "You also don't need to worry about street visibility to get clients because marketing will do that for you, so don't focus so much on the location."
Starting a Personal Training Business, out now on the Bedros Keuilian Youtube Channel, offers solutions to the most pressing issues that fitness professionals face when starting a new business. Hoping to set more personal trainers up for success, Keuilian's new video is a useful tool for those who are considering making the switch from a personal trainer to a fitness business owner and even gives examples of how to get clients into their new fitness facility.
To read more of Keuilian's expert advice on starting a personal training business, please visit his fitness marketing blog - http://ptpower.com/.
About Keuilian Inc.
Keuilian Inc. was founded by fitness marketing expert, Bedros Keuilian. Keuilian has been called the "hidden genius" behind many of the biggest names in the fitness industry - including top celebrity fitness trainers seen on television networks such as ABC and NBC. He is also the founder of the fitness marketing blog, PTPower.com, and president and CEO of the indoor fitness boot camp franchise, Fit Body Boot Camp. Keuilian is a long time business coach who has taught thousands of personal trainers how to grow their business, make more money, and get more clients.
To find out more about Bedros Keuilian and his fitness boot camp franchise, please visit http://www.ptpower.com/and http://www.myfitbodybootcamp.com/.
About Author
- Casting Roundup: Sarah Chalke, Kim Raver Headline New Pilots
- ADVENTURE TIME Headed to the Big Screen?
- Pilot Season Roundup: Colm Feore, Daniel Stern, Dave Annable to Star in New Shows
- James Franco to Star in New Fox Comedy, WHY HIM?
- Christopher Meloni to Star in WGN America's Slave Drama UNDERGROUND
- Check Out Alex Dunphy's Full College Essay from Latest MODERN FAMILY Episode
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- Syfy to Honor Leonard Nimoy with Special Programming This Sunday
- Grammy Winner Kelly Clarkson Exclusively Premieres New Music from New Album on MTV, VH1 & CMT First
- CONSTELLATIONS' Jake Gyllenhaal and Ruth Wilson Set for THEATER TALK This Weekend


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Brandon Maszon
Bio: I am a guitar player of 24 years originally hailing from Baltimore, Maryland but I have been in York, PA now for the last two years attending collage. I started "One Honest Voice" so that I can write & play heavy, progressive, melodic guitar driven metal; with the help of many of my friends in ...See Full Bio
Posted a new song: "You Haunt My Dreams " http://www.reverbnation.com/c./poni/263536206 #music


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Articles tagged under the mink
1 Jan 1995 // 1:03 AM
We are all about to embark into the dark underbelly of 1950's New York, a place where the 'straight' and 'proper' big-wigs have more to hide and infinitely much more to be ashamed of - are more 'closeted' - than the marginalized gay community...READ more
//Mixed media
Colette Carr "Static" (video) (Premiere) // Music
House of Blondes "First of July" (audio) (premiere) // Music
Digisaurus 'No More Room For Love' (EP stream) // Music
Eszter Balint 'Airless Midnight' (album stream) (premiere) // Music
//Pm Picks
PopMatters is on a short summer publishing break. We resume Monday, July 6th.
// Announcements
"PopMatters is on a short summer publishing break. We resume Monday, July 6th.READ the article


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Present address: Department of Pathology, Brigham & Women's Hospital, Harvard Medical School, Boston, MA, USA.
The Helicobacter hepaticus hefA Gene is Involved in Resistance to Amoxicillin
Article first published online: 15 JAN 2009
DOI: 10.1111/j.1523-5378.2009.00661.x
© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Ltd
Volume 14, Issue 1, pages 72–79, February 2009
Additional Information
How to Cite
Belzer, C., Stoof, J., Breijer, S., Kusters, J. G., Kuipers, E. J. and Van Vliet, A. H. M. (2009), The Helicobacter hepaticus hefA Gene is Involved in Resistance to Amoxicillin. Helicobacter, 14: 72–79. doi: 10.1111/j.1523-5378.2009.00661.x
Publication History
- Issue published online: 15 JAN 2009
- Article first published online: 15 JAN 2009
- amoxicillin;
- enteric Helicobacter species;
- treatment;
- efflux;
- antibiotic resistance;
- bile acids
Background: Gastrointestinal infections with pathogenic Helicobacter species are commonly treated with combination therapies, which often include amoxicillin. Although this treatment is effective for eradication of Helicobacter pylori, the few existing reports are less clear about antibiotic susceptibility of other Helicobacter species. In this study we have determined the susceptibility of gastric and enterohepatic Helicobacter species to amoxicillin, and have investigated the mechanism of amoxicillin resistance in Helicobacter hepaticus.
Materials and methods: The minimal inhibitory concentration (MIC) of antimicrobial compounds was determined by E-test and agar/broth dilution assays. The hefA gene of H. hepaticus was inactivated by insertion of a chloramphenicol resistance gene. Transcription was measured by quantitative real-time polymerase chain reaction.
Results: Three gastric Helicobacter species (H. pylori, H. mustelae, and H. acinonychis) were susceptible to amoxicillin (MIC < 0.25 mg/L). In contrast, three enterohepatic Helicobacter species (H. rappini, H. bilis, and H. hepaticus) were resistant to amoxicillin (MIC of 8, 16, and 6–64 mg/L, respectively). There was no detectable β-lactamase activity in H. hepaticus, and inhibition of β-lactamases did not change the MIC of amoxicillin of H. hepaticus. A H. hepaticus hefA (hh0224) mutant, encoding a TolC-component of a putative efflux system, resulted in loss of amoxicillin resistance (MIC 0.25 mg/L), and also resulted in increased sensitivity to bile acids. Finally, transcription of the hefA gene was not responsive to amoxicillin, but induced by bile acids.
Conclusions: Rodents are frequently colonized by a variety of enterohepatic Helicobacter species, and this may affect their global health status and intestinal inflammatory responses. Animal facilities should have treatment strategies for Helicobacter infections, and hence resistance of enterohepatic Helicobacter species to amoxicillin should be considered when designing eradication programs.


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Are you B Hejduková?Claim your profile
Publications (3)2.87 Total impact
Article: Changes of event related potential and cognitive processes in patients with subclinical hypothyroidism after thyroxine treatment.[Show abstract] [Hide abstract]
ABSTRACT: Recently, it has been repeatedly shown that patients with subclinical hypothyroidism (SH) formerly considered as completely symptom free, may have numerous minimal, often non-specific subjective complaints, and that in those patients it is possible to prove many subtle but objective deviations. We decided to quantify whether there are event related potential (ERP) deviations as electrophysiological markers of cognitive activity in patients with SH and whether ERP could be influenced by thyroxine treatment leading to normalization of TSH level in serum. Event related potential (ERP) was examined in thirty one patients (mean age 52 +/- 12.5 years) with SH and without any other endocrine or metabolic diseases and in 29 subjects of the control group. From 31 patients 20 women (mean age 61.8 +/-6.8 years) were selected and divided into a group of 10 women treated six months with L-thyroxine until the normalization of TSH and remaining 10 women receiving placebo. ERP examination was repeated and all such patients also underwent neuropsychological examination consisting of the Wechsler Memory Scale and the MMPI/100 (Minnesota Multiphasic Personality Inventory). The interval between the diagnosis of SH and final evaluation of treatment was 16 months. In SH thyroxine treated patients the average P3 wave latency was 374 ms (SD 40.6), while in placebo group it was 340 ms (SD 32.3. P<0.01). In addition, the treatment with thyroxine normalized the TSH level resulted in a decrease of P3 wave latency from 374 +/- 36.3 ms to 343 +/- 16.3 ms (P<0.01). However, in the placebo group such changes were not observed, the latency of P3 being 387 +/- 24.3 ms at the beginning and 379 +/- 36.5 ms at the end of observation period. No significant correlations between P3 wave latency and thyroid parameters were found. In thyroxine treated group a significant improvement in verbal memory (P<0.01), visual memory (P<0.01) and total memory scores (P<0.01) was found, while no changes in these parameters were observed in the placebo group. No significant differences were found in the MMPI test evaluation. SH patients had significantly longer P3 wave latency in ERP examination as compared to healthy individuals which gives evidence for impaired cognitive functions in SH patients. In these patients the normalization of TSH level by thyroxine treatment resulted also in the normalization of P3 wave latency. In addition, also verbal, visual and total memory scores improved significantly with the TSH normalization.Endocrine regulations 10/2002; 36(3):115-22.
Article: [Effect of normalization of an isolated increase in TSH on the neuropsychological profile of patients].[Show abstract] [Hide abstract]
ABSTRACT: Isolated TSH increase--"subclinical hypothyreosis" (SH) appears to be comparatively common. Its incidence among women over sixty has been estimated up to 17%. Though SH was assumed to be entirely asymptomatic, recent findings revealed various fine subjective and objective symptoms of the disease. Twenty otherwise healthy female patients with SH diagnosis were examined. Using personality questionnaire MMPI/100 eight different clinical scales and two control scales were evaluated, using Wechsler's memory scale WM-R 5 memory quotients were tested. The group was randomised into a subgroup treated for six month with placebo and a subgroup where thyroxin was administrated and thyroidal test normalised. Psychological testing repeated before and after the treatment, results evaluated using paired t-test. Results indicate that: 1. Patients with SH did not differ in personality features, they only had a higher tendency to the depressive mood, which could be positively influenced by thyroxin (significance at 5% level). 2. Thyroxin treatment brought about improvement of some cognitive parameters of the verbal (p < 0.01), visual (p < 0.05), and general memory (p < 0.01). 3. Results of psychological and cognitive tests correlate better with FT4 than TSH level. Though SH cannot be associated with changes in personality features, TSH normalisation results in statistically significant improvement of cognitive function--verbal, visual and general memory.Casopís lékar̆ů c̆eských 05/2000; 139(10):313-6.
Article: Amantadine infusion in treatment of motor fluctuations and dyskinesias in Parkinson's disease.[Show abstract] [Hide abstract]
ABSTRACT: Efficiency and safety of amantadine sulfate (AMS) infusions were investigated in late stage complications of Parkinson's disease (PD). In an open-label study, 21 PD patients suffering from motor fluctuations and/or dyskinesias were administered AMS infusions (PK-Merz, 400 mg per day) during seven days. Oral AMS treatment followed. Significant improvement of UPDRS motor scores was observed between day 0 and day 7, remaining improved until day 21. Based on patients' diary notes, both severity and occurrence of hypokinetic "off" state significantly decreased (from 6.6 to 3.1 hours, p < 0.001, average "off" time per day) as well as dopaminergic-induced dyskinesias (from 2.5 to 1.3 hours, p < 0.05, average duration of dyskinesias per day). AMS infusions followed by oral administration appeared as a safe method for improvement of both motor fluctuations and dyskinesias in advanced PD. In advantage to simple oral therapy, AMS infusions allowed fast introduction of a profound and durable treatment effect.Journal of Neural Transmission 02/2000; 107(11):1297-306. DOI:10.1007/s007020070019 · 2.87 Impact Factor
Top Journals
Military University Hospital PraguePraha, Praha, Czech Republic
Charles University in Prague
Praha, Praha, Czech Republic
- 1st Faculty of Medicine


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|By Marketwired .
Article Rating:
|August 29, 2014 02:43 PM EDT
VANCOUVER, BRITISH COLUMBIA -- (Marketwired) -- 08/29/14 -- Abzu Gold Ltd. ("Abzu" or the "Company") (TSX VENTURE:ABS) announces the resignation of Mr. Wilson Soon as Chief Financial Officer of the Company. While the Company continues to interview candidates as successor to Mr. Wilson, Mr. Leonard Buerk will serve as Acting Chief Financial Officer in a temporary capacity.
The Company would like to thank Mr. Wilson for his contributions to the Company and wishes him well in his future endeavours.
On behalf of the board of directors of ABZU GOLD LTD.
Bob Baxter, CEO & President
Neither TSX Venture Exchange nor its Regulation Services Provider (as that term is defined in the policies of the TSX Venture Exchange) accepts responsibility for the adequacy or accuracy of this press release, which has been prepared by management.
Abzu Gold Ltd.
Bob Baxter
CEO & President
[email protected]
In the midst of the widespread popularity and adoption of cloud computing, it seems like everything is being offered "as a Service" these days: Infrastructure? Check. Platform? You bet. Software? Absolutely. Toaster? It's only a matter of time. With service providers positioning vastly differing offerings under a generic "cloud" umbrella, it's all too easy to get confused about what's actually being offered. In his session at 16th Cloud Expo, Kevin Hazard, Director of Digital Content for SoftL...
Jul. 7, 2015 01:45 PM EDT Reads: 2,592
SYS-CON Events announced today that Alert Logic, the leading provider of Security-as-a-Service solutions for the cloud, has been named "Bronze Sponsor" of SYS-CON's 17th International Cloud Expo® and DevOps Summit 2015 Silicon Valley, which will take place November 3–5, 2015, at the Santa Clara Convention Center in Santa Clara, CA. Alert Logic provides Security-as-a-Service for on-premises, cloud, and hybrid IT infrastructures, delivering deep security insight and continuous protection for cust...
Jul. 7, 2015 01:45 PM EDT Reads: 2,430
The cloud has transformed how we think about software quality. Instead of preventing failures, we must focus on automatic recovery from failure. In other words, resilience trumps traditional quality measures. Continuous delivery models further squeeze traditional notions of quality. Remember the venerable project management Iron Triangle? Among time, scope, and cost, you can only fix two or quality will suffer. Only in today's DevOps world, continuous testing, integration, and deployment upend...
Jul. 7, 2015 01:30 PM EDT Reads: 2,715
Countless business models have spawned from the IaaS industry. Resell Web hosting, blogs, public cloud, and on and on. With the overwhelming amount of tools available to us, it's sometimes easy to overlook that many of them are just new skins of resources we've had for a long time. In his General Session at 16th Cloud Expo, Phil Jackson, Lead Technology Evangelist at SoftLayer, broke down what we've got to work with and discuss the benefits and pitfalls to discover how we can best use them to d...
Jul. 7, 2015 01:30 PM EDT Reads: 2,245
Public Cloud IaaS started its life in the developer and startup communities and has grown rapidly to a $20B+ industry, but it still pales in comparison to how much is spent worldwide on IT: $3.6 trillion. In fact, there are 8.6 million data centers worldwide, the reality is many small and medium sized business have server closets and colocation footprints filled with servers and storage gear. While on-premise environment virtualization may have peaked at 75%, the Public Cloud has lagged in adop...
Jul. 7, 2015 01:30 PM EDT Reads: 2,569
SYS-CON Media announced today that CloudBees, the Jenkins Enterprise company, has launched ad campaigns on SYS-CON's DevOps Journal. CloudBees' campaigns focus on the business value of Continuous Delivery and how it has been recognized as a game changer for IT and is now a top priority for organizations, and the best ways to optimize Jenkins to ensure your continuous integration environment is optimally configured.
Jul. 7, 2015 01:15 PM EDT Reads: 1,421
SYS-CON Events announced today that Solgenia will exhibit at SYS-CON's 17th International Cloud Expo®, which will take place on November 3–5, 2015, at the Santa Clara Convention Center in Santa Clara, CA. Solgenia is the global market leader in Cloud Collaboration and Cloud Infrastructure software solutions. Designed to "Bridge the Gap" between Personal and Professional Social, Mobile and Cloud user experiences, our solutions help large and medium-sized organizations dramatically improve produc...
Jul. 7, 2015 01:00 PM EDT Reads: 2,216
DevOps tends to focus on the relationship between Dev and Ops, putting an emphasis on the ops and application infrastructure. But that's changing with microservices architectures. In her session at DevOps Summit, Lori MacVittie, Evangelist for F5 Networks, will focus on how microservices are changing the underlying architectures needed to scale, secure and deliver applications based on highly distributed (micro) services and why that means an expansion into "the network" for DevOps.
Jul. 7, 2015 01:00 PM EDT Reads: 3,113
Live Webinar with 451 Research Analyst Peter Christy. Join us on Wednesday July 22, 2015, at 10 am PT / 1 pm ET In a world where users are on the Internet and the applications are in the cloud, how do you maintain your historic SLA with your users? Peter Christy, Research Director, Networks at 451 Research, will discuss this new network paradigm, one in which there is no LAN and no WAN, and discuss what users and network administrators gain and give up when migrating to the agile world of clo...
Jul. 7, 2015 01:00 PM EDT Reads: 1,777
Connected things, systems and people can provide information to other things, systems and people and initiate actions for each other that result in new service possibilities. By taking a look at the impact of Internet of Things when it transitions to a highly connected services marketplace we can understand how connecting the right "things" and leveraging the right partners can provide enormous impact to your business' growth and success. In her general session at @ThingsExpo, Esmeralda Swartz...
Jul. 7, 2015 12:45 PM EDT Reads: 1,656
"Plutora provides release and testing environment capabilities to the enterprise," explained Dalibor Siroky, Director and Co-founder of Plutora, in this SYS-CON.tv interview at @DevOpsSummit, held June 9-11, 2015, at the Javits Center in New York City.
Jul. 7, 2015 12:45 PM EDT Reads: 1,891
The 17th International Cloud Expo has announced that its Call for Papers is open. 17th International Cloud Expo, to be held November 3-5, 2015, at the Santa Clara Convention Center in Santa Clara, CA, brings together Cloud Computing, APM, APIs, Microservices, Security, Big Data, Internet of Things, DevOps and WebRTC to one location. With cloud computing driving a higher percentage of enterprise IT budgets every year, it becomes increasingly important to plant your flag in this fast-expanding bu...
Jul. 7, 2015 12:30 PM EDT Reads: 1,934
The 5th International DevOps Summit, co-located with 17th International Cloud Expo – being held November 3-5, 2015, at the Santa Clara Convention Center in Santa Clara, CA – announces that its Call for Papers is open. Born out of proven success in agile development, cloud computing, and process automation, DevOps is a macro trend you cannot afford to miss. From showcase success stories from early adopters and web-scale businesses, DevOps is expanding to organizations of all sizes, including the ...
Jul. 7, 2015 12:15 PM EDT Reads: 1,965
In their general session at 16th Cloud Expo, Michael Piccininni, Global Account Manager - Cloud SP at EMC Corporation, and Mike Dietze, Regional Director at Windstream Hosted Solutions, reviewed next generation cloud services, including the Windstream-EMC Tier Storage solutions, and discussed how to increase efficiencies, improve service delivery and enhance corporate cloud solution development. Michael Piccininni is Global Account Manager – Cloud SP at EMC Corporation. He has been engaged in t...
Jul. 7, 2015 12:15 PM EDT Reads: 2,417
The most often asked question post-DevOps introduction is: "How do I get started?" There's plenty of information on why DevOps is valid and important, but many managers still struggle with simple basics for how to initiate a DevOps program in their business. They struggle with issues related to current organizational inertia, the lack of experience on Continuous Integration/Delivery, understanding where DevOps will affect revenue and budget, etc. In their session at DevOps Summit, JP Morgenthal...
Jul. 7, 2015 12:00 PM EDT Reads: 1,675


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Big Game® 19' Arch Rival Ladder Tree Stand
Item # WX2-222711
- Google+
- Details & Specs
Big Game®Arch RivalTM 19' Ladder Tree Stand is your new perch so you can spot deer coming.
Double Tree Stand deal! Wow, 19' up to the shooting rail! Plus, this Stand has a roomy 25 x 24" mesh platform. And the seat is to die for... comfortable as a throne in the sky, complete with an attached backrest. Gets you way up there!Good stuff:
- Sturdy steel construction
- 4 fit-together single rail sections
- Big 25 x 24"d. mesh foot platform
- Foot rest silently flips back for full platform use
- 20"h. seat (26 x 13"d.) flips up for full platform use
- Seat cushion is 26 x 14"d., 2" thick
- Attached backrest
- Padded, adjustable shooting rail flips up for bow use and is removable
- Has one 1" ratchet strap, two 1" stabilizer straps for rock-steady support
- Includes adjustable support bar and a 4-pt. full-body fall-arrest harness
- 9" minimum tree diam.
- 300-lb. weight capacity.
- 64 lbs.
Please Note : This is a Heavy / Bulky item. $10.00 for heavy / bulky shipping and handling will be charged in addition to regular shipping and handling.
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What is a class? I have variously heard classes described as archetypes, as like an occupation a character trains for, or as a methodology for solving problems. All of these descriptions are useful, and I don't think there has to be a precise definition. But if D&D is to be an appealing class based role-playing system, I think the classes do have to be meaningful. They must serve a natural function in categorizing characters: class features should arise naturally from the archetype/occupation/methodology and not as arbitrary rules. Thus any character conceivable that fits the archetype should fit the class, not just certain personalities and backgrounds. Actions chosen should be driven by the character being played, not the class. So far, I do not expect strong disagreement – but on to the controversial parts of this post.
1. "Rogue" is NOT a class; it is a personality. Surely a character can be inclined toward discretion and subtle physical talents as a means of solving problems while still acting with honor and strict adherence to legal principles. And one can act irresponsibly, on the fringes of legal and or accepted behavior, with crude manners or as a dishonest charlatan while solving conflicts through brute strength and mastery of weapons with the protection of heavy armor.
2. "Barbarian" is NOT a class; it is a background. Surely a character can come from a technologically backward society outside the mainstream cultural norms and still approach problems, say, with a strong faith in the divine and through pursuit of the transcendental. On the other hand, even a highly refined paragon of social propriety can have a deep seated, thinly controlled rage when confronted with conflict.
3. "Monk" does NOT imply lawful. Surely one can exhibit high levels of personal discipline, self-denial, and rigorous training while still upholding the supremacy of the individual and opposing laws that suppress free personal expression – even to the point of opposing all laws in favor of personal responsibility (an anarchist view).
4. "Ranger" is NOT a class; it is a type of fighter. It is a person who has applied martial training and techniques is ways adapted to wilderness situations.
I recognize WOTC will probably be unwilling to address these complaints – there are some strong D&D traditions that are hard to change; eliminating rogue, barbarian, and ranger classes will turn off more customers than it pleases. But I would argue that there are traditions (e.g. THAC0) that need to change for the game to improve. If D&D next does not strive to improve upon – rather than to just re-create the game to sell new materials, it is destined to fail.


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Beef Burgers in Red Wine Sauce
17 Nov 2010
|1 Small
|Minced beef
|12 Ounce (350 Gram)
|Fresh white breadcrumbs
|2 Ounce (50 Gram)
|Chopped parsley
|15 Milliliter (1 Tablespoon)
|1 , beaten
|To Taste
|To Taste
|45 Milliliter (3 Tablespoon)
|Canned homepride red wine cook in sauce
|13 1⁄4 Ounce (1 Can)
|Watercress sprig
|4 (To Garnish)
Peel and finely chop the onion.
Mix together the onion, minced beef, breadcrumbs, parsley, egg, salt and pepper in a bowl.
Divide the mixture into 8 and shape into burgers.
Heat the oil in a medium-sized pan and fry the burgers for 7 minutes until brown, turning once.
Drain off the oil, add the Red Wine Cook-in-Sauce, cover and simmer for 30 minutes, stirring occasionally.
Serve on rice or with creamed potatoes and garnish with watercress.
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Beef Burgers In Red Wine Sauce Recipe


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Plans are being made for our Vacation Bible School on July 13-17. Click here for more information!
Our sanctuary is equipped with headsets as well as an induction hearing loop for those with 'T-Coil' style hearing aids.
Our mission is to build a caring congregation that proclaims God's glory in Jesus Christ through relevant preaching and teaching, warm community life, and relational outreach.


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The defending Division 1 state champion Germantown boys basketball team has won 32 games in a row and finds itself atop the MyCommunityNOW/Lake Country Roundball Rankings.
The Hamilton Chargers are the top-ranked girls team and are coming off a 64-10 victory over West Allis Central in the Greater Metro Conference opener.
In addition to the top five boys and girls teams in the area, Roundball Rankings includes a player of the week, poll, area scoring leaders and more.
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Alright ladies, here is the new thread since the other one was near death. New buddies welcome also! Most of us here having been following eachother since Clomid round one, and it's round three or four for most of us. But you are welcome to join whether it's your first round or your sixth! :) Hoping and praying for some BFP's this time!
UPDATE! Here is our BFP list so far, hoping to watch it grow!!!!


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TAYLOR SWIFT BRINGS THE 1989 WORLD TOUR TO SHANGHAI The 1989 World Tour is Presented in China by Toyota Hybrid Mercedes-Benz Arena – Shanghai – November 10 & 11, 2015 TICKETS ON SALE JUNE 30 at 2PM
Everything Has Changed Music Video!
Check out the music video for "Everything Has changed" below!
TAYLOR SWIFT BRINGS THE 1989 WORLD TOUR TO SINGAPORE Singapore Indoor Stadium – 7 & 8 November, 2015 TICKETS ON SALE 30 JUNE at 10AM (24 June, 2015, Singapore) – Global superstar Taylor Swift announced today she is returning to Singapore,...
I write this to explain why I'll be holding back my album, 1989, from the new streaming service, Apple Music. I feel this deserves an explanation because Apple has been and will continue to be one of my best partners in selling music and creating ways for me to connect with my fans. I...
Fans attending The 1989 World Tour in Detroit got TWO special suprises on Saturday! Dan Reynolds from the GRAMMY Award winning band Imagine Dragons joined Taylor for a special duet performance of the hit song "Radioactive." Fellow "Bad Blood" co-stars and...


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Therapists in Berkeley
Verified by Psychology Today
Shauna Reiff
Marriage & Family Therapist, MS, MFT
"Dissatisfied with your relationship to a partner, boss, or yourself? Do things feel out of control? Finding a good fit with a therapist can play a vital role in getting more of what you want in life. I see myself as someone who guides and facilitates a committed, compassionate and confidential space where we can clarify what is most important to you, and discover the blocks that get in the way of having the life that you want. In my practice I help individuals and couples find their own way to lasting changes.
(510) 986-4368
"If you'd like to find peace in your body or free up some elusive pattern, somatic (body-based) therapy may be what you're looking for. My sessions blend gentle hands-on, movement, interactive imagery, and verbal guidance with warmth, safety, and good-hearted humor. I support your becoming present to your body/Self without judgement. As you reconnect nonverbally with yourself, stories and emotional knots hidden during talk therapy often reveal and resolve themselves. Working through the body, you'll learn to align body and heart, recognize new possibilities, resolve issues, feel better physically, and live more authentically.
(510) 240-1809
Verified by Psychology Today
Bill Burmester
Marriage & Family Therapist, MA, MFT
"In private practice in Berkeley, California, I work with individuals (teens and adults), couples, and groups on recovering from trauma. Trauma can be the recurring intrusive impact of specific overwhelming past events or the cumulative disruptive effect of inadequate nurture and protection in childhood. Since 1991, I have worked in particular with male survivors of sexual abuse. From a base of safety, support, and respect, I work collaboratively with my clients to pace their pursuit of psychological and somatic healing and growth, drawing on the strengths and resources everyone brings with them into therapy.
(510) 574-7508
Berkeley, California 94710
"I have years experience working with children, families and parenting. I also specialize in working with people with illness. I have an eclectic approach to treatment including narrative therapy, coaching, imagery work and hypnotherapy, as well as play therapy. I am very interested in helping people get out of their "stuck" places and work on realizing their life dreams and goals.l work with the impact of illness on everyday life. Another of my specialties is working with children and families. I have play activities, art materials, and sandtray in my offices and enjoy connecting with children as well as working with family dysfunction.
(510) 478-9653
Berkeley, California 94710
"I provide diagnostics through psychological and neuropsychological evaluation; and therapy. My experience includes over twenty years in the field of human services focusing on under-served individuals to increase access to support and facilitate empowerment. Therapy, evaluation, and other means of self-exploration allow an opportunity to become more familiar with our strengths, clarify goals, and define directions for growth. My approach is short term and pragmatic for those that have identified specific needs and wish to achieve a particular objective(s). Others may desire more on-going support and exploration.
(510) 239-5325
Verified by Psychology Today
Annie Wright
Marriage & Family Therapist Intern, MA, MFTi
"So why therapy? Well, when you're going through yet another breakup, or are waking up each morning dreading going into work, or if you're dealing with a serious case of Instagram envy and "time-is-passing-me-by-and -I've-got-to-change-my-life!" anxiety, times like these can be so challenging and you may be feeling overwhelmed, stuck, sad, angry, and (especially if it's been like this for some time!) probably more than a little confused and hopeless about how to change things and move forward towards the life you actually envision for yourself.
(510) 254-3196
Berkeley, California 94703
Verified by Psychology Today
Michael Mayer
Psychologist, PhD
"Real transformation is possible when you find your connection with your authentic Self.. With my 30 years experience, I give you leading-edge methods to help you deal with various psychological issues. Self-healing tools from Eastern and Western traditions can reduce or eliminate medications and empowers your inner healer to deal with your anxiety, depression, chronic pain, insomnia, hypertension, etc.
(510) 878-4268
Verified by Psychology Today
Carrie Katz
Marriage & Family Therapist, LMFT, MA
"Are you wanting to feel more fulfilled in your life and relationships? I help people create change around their depression and anxiety, and move through times of big change, loss, or feelings of "stuckness". If you are looking for a therapist who will be supportive but who will also gently challenge you to move beyond limiting patterns in a non-judgmental way, we might be a good fit. My approach is based in somatic (body-oriented) therapy, including mindfulness, which can help in ways that talk therapy may not be able to.
(510) 344-6318
Berkeley, California 94703
Verified by Psychology Today
Kathleen S Pierce
Psychologist, PsyD
"I have extensive experience working with survivors of trauma, both military and civilian (since 1995). Substance Use disorders are often an unwelcomed side-effect of trauma, and I can work compassionately when they co-occur. My clinical experience spans across the lifespan. I understand the unique challenges that developmental age plays re: traumatic events, death and loss. I take a gentle yet proactive approach to treating trauma. I know the difficulty of talking about these most personal and often horrifying events. I have compassion and respect for survivors of trauma. My approach to treating trauma is reflective of this.
(510) 246-3479
Verified by Psychology Today
Kelley Bryan Gin
Psychologist, PsyD
"I have nearly 20 years treating children, teens, and families facing complicated emotional and behavioral conditions. How I work depends upon the circumstances bringing you to a psychotherapist as different problems and situations need different approaches. Every therapy is unique even if there are preferred methods and practices for treating anxiety, depression, and others. Each treatment is foremost a relationship. This often takes time, which may be a challenge when limited by schedules, insurance benefits, or circumstances. My goal is that psychotherapy is effective in addressing change in thinking and behavior, and that you find it helpful.
(510) 403-1450
Verified by Psychology Today
Alexandra Elite-Marcandonatou
Clinical Social Work/Therapist, LCSW
"I provide psychotherapy to individuals utilizing a holistic approach that incorporates mindfulness-based cognitive therapy and is inspired by humanistic, psychodynamic, relational and attachment theories. Using an integrative approach, treatment is customized to meet your individual needs/interests. We can work together to gain a deeper understanding of yourself, your relationships and the way in which you respond to the stresses, demands and challenges you experience in your life. Along these lines, you will have the opportunity to explore relational patterns that may be getting in the way of deeper satisfaction and joy.
(415) 969-7895
Verified by Psychology Today
Orit Weksler
Marriage & Family Therapist, MFT
"My training is in Expressive Arts Therapy, Psychoanalytic psychotherapy and EMDR. This combination allows me to work deeply and creatively, assessing your unique needs throughout the time of our work together. Therapy is a collaborative process: it happens in a space/time that is removed from the regular occurrences of life. As such, it is an opportunity to play around with ideas, thoughts and feelings in a safe setting.I take our work together very seriously, but do my best not to take myself too seriously. A sense of humor is an important component of this work, and of life.
(510) 356-2783
Verified by Psychology Today
Ellen Case
Marriage & Family Therapist, MA, MFA, MFT
"Surviving but not thriving? I can help you get to the root of your issues, change negative or addictive thinking patterns, transform your sense of self, relationships, work life, and creativity, while building hope and self-confidence. My style is interactive, down-to-earth, collaborative, and respectful. I use relational, cognitive and psychodynamic approaches; I ask meaningful questions, listen closely, and offer new perspectives. I also work to tailor a therapy plan that suits your unique core issues and goals. I look forward to talking with you about making deep, lasting and positive changes in your life.
(510) 972-1353
Verified by Psychology Today
Shira Gallagher
Clinical Social Work/Therapist, LCSW
"Working with a therapist is a unique process that can allow us to see ourselves and situations from a new perspective, better understand ourselves, and transform things that we wish to change. As the therapist, I listen deeply and engage with you to help us explore what is going on within you that is troubling or limiting you and help you make meaningful and lasting changes. The foundation for effective therapy is a safe, respectful space in which thoughts and feelings can be explored collaboratively. In my work with adults and adolescents, my style is empathic, responsive, and non-judgmental.
(925) 695-7982
Verified by Psychology Today
Kim Hraca
Marriage & Family Therapist
"Self-acceptance can help you make lasting changes in your life. I offer insight, a sense of humor, and a safe and supportive place to experience yourself (and your partner) with more compassion and self understanding. Together we can explore issues of self-image, intimacy, work satisfaction, creativity, and spirituality. Couples are also welcome. My work as a psychotherapist incorporates certain guiding beliefs: 1) Our world view results from our childhood experiences; this world view greatly affects our current lifestyle, self-image and interaction with others; 2) Our ability to change largely depends on making this world view conscious, so
(510) 601-1859
Verified by Psychology Today
Janice A Schmidt
Marriage & Family Therapist, LMFT
"Ready to thrive in your life? I provide therapy for people who want to change unhealthy patterns. People who have worked with me say they feel safe and compassionately supported. My presence and guidance can assist you in making small but significant changes in your life, create a deeper understanding of yourself and your emotional states, and clarify your thought patterns. Together, we will identify and engage your intuition, and develop your personal strengths providing resilience.
(510) 497-0058
Verified by Psychology Today
Pamela Zelnik
Marriage & Family Therapist, LMFT
"I bring a warm, supportive, practical and interactive approach to working with my clients. Working together, we uncover and identify your concerns and obstacles as well as strengths, hopes and pleasures. Feeling stuck, confused, in limbo or in transition can be so painful. With focus and support, taking those first few steps can feel more doable as you try new strategies and skills for managing difficult challenges, improving well being, and leading a fuller life.
(510) 398-6336
Berkeley, California 94707
Jill Horowitz
Clinical Social Work/Therapist, LCSW, BCD, Profess, Clinica, P
"I provide a very safe setting for people to reveal their hopes, anxieties and deepest fears. Whether you're coming with a current crisis or after years of trying to help yourself or get help, out of our sensitively uncovering together what underlies your issues and the impasses, new ways of seeing, feeling and being emerge. Through my many years of experience, education and training, I've been able to help many people substantially improve the quality of their lives and relationships. (Potential clients/ patients often ask if I stay silent!---I don't! We will do the work together!)
(510) 848-8083
Verified by Psychology Today
Ethan Schwartz
Psychologist, PhD
"My work involves collaborating with individuals and couples on their own personal recipes for life satisfaction and what their lives will look like when the "problem" is no longer a problem. I try to carefully separate the "problem" from the person, who is often doing the best he or she can, so we can identify strengths, resiliencies, and resources that allow clients to construct a life worth living and a relationship worth celebrating. This does not mean ignoring difficult issues or painful areas, but it is important to know at the outset how we will know therapy has been successful.
(510) 878-6472
Verified by Psychology Today
Jonathan Burstein
Marriage & Family Therapist, MA, LMFT
"Therapy is a journey of self-discovery in the service of healing, insight, and change. As your therapist, I am a dedicated partner and a collaborator on this journey. I approach our work with a warm, compassionate interest in you, your life stories, and the various ways you try to solve life's challenges. We look together for untapped strengths and resources, unhelpful and unconscious patterns, and new ways of relating to yourself and the others in your life. Together we come to new understandings and new experiences that will directly influence your life outside of the therapy room.
(510) 982-6202
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Try expanding your search for Therapists in Berkeley to a larger area (e.g. Alameda County, California)
How can I tell if a therapist is right for me?
Therapists in Berkeley have broad skills and are able to work with a wide range of issues.
For example, if you're seeking a marriage counselor in Berkeley you'll find that most therapists are trained in marriage counseling or couples counseling in Berkeley and couples therapy. And they welcome families for family counseling in Berkeley or family therapy in Berkeley.
How to find a specialist?
If you're looking for specialist counseling in Berkeley, California, such as a child psychologist or sex therapist, try to refine your search with our left nav bar.
What method of therapy is right for me?
When it comes to treatment methods, there's no "right way". A couples counselor or a family counselor may use different treatment methods depending on the one that works best for you.
How to use my insurance?
Many therapists accept health insurance. Check to see if your insurance is covered. Feel free to discuss this when you contact the therapist.


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http://www.youtube.com/watch?v=IYWDsHpCmSk - PLEASE WATCH THE VIDEO BEFORE EMAIL ABOUT HER UNDERSADDLE, THIS VIDEO WAS HER LAST DAY AT THE TRAINERS (LESS…SOLD
All Around, Barrel Racing, English Pleasure, Halter, Hunter, Reining, Show, Trail Riding, Western Pleasure, Working Cattle
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The necktie is a salute to Joe.
Wonder if their white pants have a brown stripe.
Member since 08 December 2013 | Blog
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Voting Record: 2022 / 172
The necktie is a salute to Joe.
Wonder if their white pants have a brown stripe.
Cowherd said late today that he will have more on the worst interview in history tomorrow.
Hope DJ or someone posts his comments.
According to the Bengals no one is allowed to document, via video, accounts of those full-team drills.and Pryor posted images from their recent minicamp to one of his social media accounts Monday afternoon.
Bengals spokesmen said: "If I'm [another team], I have to think hard about signing him or else he might give out valuable information of theirs, too."
I think you will see Marshall and Wilson mostly at receiver. Dunn backs up Zeke. Samuel at H. Of course there will be some playing around with this and injuries matter too. But the thing I know for sure is, YOU WANT SAMUEL ON THE FIELD.
Indeed a reboot is needed.
Seems like the last several years Buckeyes have had one great player and some guys who can't shoot worth a damn.
What I find fascinating is that the Spartans/Dantonio still ride the "play with violence because no one respects you" pony.
I suspect they could win a National Championship, return practically everyone and still sell it.
I am rooting for the Cavs but hate to say I don't think they can keep up this pace. Remaining games will be be played on fumes and I think Curry will take over.
From the looks of the voting you are not alone.
I remember reading a story that Jim Otis just blasted a team, that I don't now recall. A sportswriter from that team's city immediately after the game was riding up an elevator at OSU. He looked down and exclaimed, "Oh my God even the elevators are Otis.
My lord how could you keep any of these three out of the Hall?!
Dodd has lost some gray matter along with his hair.
I remember when another highly touted QB decided to stay a Buckeye even though most pundits were saying sit a year and play for another school. It was Troy Smith who got the position over Justin Zwick. All Zwick had to say was that there are great opportunities presented to those in the Buckeye family outside of football.
Galloway was the first in a long line that started the phrase known in many circles as "Ohio State is wide receiver U".
If they win with that season ending gauntlet of MSU, TTUN, B1G championship, and two playoff games, they will be one of the best college football teams in history.
Muchas gracias!
Caught some of the Virginia Tech game. Thought I was in the Twilight Zone.
Agree, and it's not only that, but Coach D has successfully cultivated an attitude of "they don't respect us so lets make them". Wonder if there is a point where that doesn't play well anymore.
In fairness, Fred Taylor retired from coaching in 1976 at the age of 52. I remember him well and always watched his television show, and followed his career. I know with a fair amount of certainty that he was "disenchanted", to say the least, with coaching after the 1972 brawl at Minnesota that left three Ohio State players hospitalized. Taylor always felt that the Ohio State administration and Big Ten should have pushed for more aggressive disciplinary action against those Minnesota players involved in that public mugging.
"I still kick myself for not quitting then because that's what I felt I should do when nobody really even acted as though they cared," Taylor said later. His heart for the game he loved was gone, and it was obvious.
I am a Browns fan too and would love to see Pryor start, especially two games a year.
What worries me is that it's damn near impossible for a football team to play at peak levels for 5 games in a row. It's human nature to think that your team mates will have the necessary intensity and focus when your mind and body is not responding at it's best. Then it's all too common to find that things are not clicking for a couple of other guys also, while your opponent is hitting on all cylinders.
Really want the Buckeyes to repeat, so my hope is that the team is just head and shoulders above everyone in college football and plays the last 5 enraged.
If you listened to Tom Herman's interview in Wednesday's Skull Session you will find that he thinks very highly of Mariota and his skill set.
I am torn between Miller and Jones.
I fully expected Braxton to lead us into the playoffs last year because he was the one guy who could make up for other team weaknesses and was electric. Defenses could not game plan for him. When he went down all I could say to my friends was to repeat the f word over and over.
On the other hand Cardale stretched defenses with that strong arm while being accurate and he doesn't go down with arm tackles. The running game with Zeke benefited and really took off.
The loser here is JT. Great leader, solid everywhere in his game. But the two in front of him are just too dynamic.
Really liked this movie. The overall plot together with the conceptualizing of an existence in not one but two other dimensions of consciousness was unique. Also thought the visuals and audio were very good, the ideas stimulating and it held my attention despite its length. If I were to criticize anything it might be that McConaughey seemed to be overacting early on.
I have been quite loose with the DVs in the past and apologize to those that may have irritated me. Unless of course you posted a political opinion, then I wish I had more than one DV to give because only a moron football fan would think his political opinion is worth a damn!
Coach Mick is just as much an important cog in the Big Buckeye Machine as any other assistant coach, and lord knows we have some of the best.
The original video had the part where the ceremony was over but the President saw Archie as he was leaving, so he started it up again. I loved that part and you can catch it on Youtube at about the 16:25 mark:
"Houston is now my third-favorite team. (My second-favorite is whoever is playing Michigan, obvii.)"
But but DJ, don't you want TTUN to return to greatness so we can return to those competitive games of yesteryear and maybe let the conference have more bragging rights?


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Pinkalicious: Tickled Pink (Paperback)
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When Tiffany challenges Pinkalicious to a laugh-off, the pressure is on to create the most pinkerrifically funny joke of all time.
Even if Pinkalicious doesn't win the contest, she's going to have a lot of fun trying!
About the Author
Victoria Kann is the award-winning illustrator and author of the picture book series featuring the whimsical and effervescent character Pinkalicious. Victoria coauthored and illustrated the first two books, Pinkalicious and Purplicious, and cowrote the play Pinkalicious: The Musical. She wrote and illustrated the New York Times number-one bestsellers Goldilicious, Silverlicious, and Emeraldalicious, as well as Aqualicious. Currently she is working on several more books about the adventures and antics of Pinkalicious. Readers can follow Pinkalicious on Facebook and Twitter.


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Are you Sadaf-Ahmahni Hussain?Claim your profile
Publications (6)30.52 Total impact
- SourceAvailable from: Federico Carafoli[Show abstract] [Hide abstract]
ABSTRACT: The heterotrimeric laminins are a defining component of basement membranes and essential for tissue formation and function in all animals. The three short arms of the cross-shaped laminin molecule are composed of one chain each and their tips mediate the formation of a polymeric network. The structural basis for laminin polymerisation is unknown. We have determined crystal structures of the short-arm tips of the mouse laminin β1 and γ1 chains, which are grossly similar to the previously determined structure of the corresponding α5 chain region. The short-arm tips consist of a laminin N-terminal (LN) domain that is attached like the head of a flower to a rod-like stem formed by tandem laminin-type epidermal growth factor-like (LE) domains. The LN domain is a β-sandwich with elaborate loop regions that differ between chains. The γ1 LN domain uniquely contains a calcium binding site. The LE domains have little regular structure and are stabilised by cysteines that are disulphide-linked 1-3, 2-4, 5-6 and 7-8 in all chains. The LN surface is not conserved across the α, β and γ chains, but within each chain subfamily there is a striking concentration of conserved residues on one face of the β-sandwich, while the opposite face invariably is shielded by glycans. We propose that the extensive conserved patches on the β and γ LN domains mediate the binding of these two chains to each other, and that the α chain LN domain subsequently binds to the composite β-γ surface. Mutations in the laminin β2 LN domain causing Pierson syndrome are likely to impair the folding of the β2 chain or its ability to form network interactions.PLoS ONE 07/2012; 7(7):e42473. DOI:10.1371/journal.pone.0042473 · 3.53 Impact Factor
Article: Determinants of laminin polymerization revealed by the structure of the α5 chain amino-terminal region[Show abstract] [Hide abstract]
ABSTRACT: The polymerization of laminin into a cell-associated network--a key step in basement membrane assembly--is mediated by the laminin amino-terminal (LN) domains at the tips of the three short arms of the laminin αβγ-heterotrimer. The crystal structure of a laminin α5LN-LE1-2 fragment shows that the LN domain is a β-jelly roll with several elaborate insertions that is attached like a flower head to the stalk-like laminin-type epidermal growth factor-like tandem. A surface loop that is strictly conserved in the LN domains of all α-short arms is required for stable ternary association with the β- and γ-short arms in the laminin network.EMBO Reports 02/2011; 12(3):276-82. DOI:10.1038/embor.2011.3 · 7.86 Impact Factor
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ABSTRACT: Dystroglycan is a ubiquitously expressed cell adhesion protein. Its principal role has been determined as a component of the dystrophin-glycoprotein complex of muscle, where it constitutes a key component of the costameric cell adhesion system. To investigate more fundamental aspects of dystroglycan function in cell adhesion, we examined the role of dystroglycan in the dynamics and assembly of cellular adhesions in myoblasts. We show that beta-dystroglycan is recruited to adhesion structures and, based on staining for vinculin, that overexpression or depletion of dystroglycan affects both size and number of fibrillar adhesions. Knockdown of dystroglycan increases the size and number of adhesions, whereas overexpression decreases the number of adhesions. Dystroglycan knockdown or overexpression affects the ability of cells to adhere to different substrates, and has effects on cell migration that are consistent with effects on the formation of fibrillar adhesions. Using an SH3 domain proteomic screen, we identified vinexin as a binding partner for dystroglycan. Furthermore, we show that dystroglycan can interact indirectly with vinculin by binding to the vinculin-binding protein vinexin, and that this interaction has a role in dystroglycan-mediated cell adhesion and spreading. For the first time, we also demonstrate unequivocally that beta-dystroglycan is a resident of focal adhesions.Journal of Cell Science 01/2010; 123(Pt 1):118-27. DOI:10.1242/jcs.047902 · 5.33 Impact Factor
Article: Structural and functional analysis of slit and heparin binding to immunoglobulin-like domains 1 and 2 of Drosophila Robo.[Show abstract] [Hide abstract]
ABSTRACT: Recognition of the secreted protein Slit by transmembrane receptors of the Robo family provides important signals in the development of the nervous system and other organs, as well as in tumor metastasis and angiogenesis. Heparan sulfate (HS) proteoglycans serve as essential co-receptors in Slit-Robo signaling. Previous studies have shown that the second leucinerich repeat domain of Slit, D2, binds to the N-terminal immunoglobulin-like domains of Robo, IG1-2. Here we present two crystal structures of Drosophila Robo IG1-2, one of which contains a bound heparin-derived oligosaccharide. Using structure-based mutagenesis of a Robo IG1-5 construct we identified key Slit binding residues (Thr-74, Phe-114, Arg-117) forming a conserved patch on the surface of IG1; mutation of similarly conserved residues in IG2 had no effect on Slit binding. Mutation of conserved basic residues in IG1 (Lys-69, Arg-117, Lys-122, Lys-123), but not in IG2, reduced binding of Robo IG1-5 to heparin, in full agreement with the Robo-heparin co-crystal structure. Our collective results, together with a recent crystal structure of a minimal human Slit-Robo complex ( Morlot, C., Thielens, N. M., Ravelli, R. B., Hemrika, W., Romijn, R. A., Gros, P., Cusack, S., and McCarthy, A. A. (2007) Proc. Natl. Acad. Sci. U.S.A. 104, 14923-14928 ), reveal a contiguous HS/heparin binding surface extending across the Slit-Robo interface. Based on the size of this composite binding site, we predict that at least five HS disaccharide units are required to support Slit-Robo signaling.Journal of Biological Chemistry 07/2008; 283(23):16226-34. DOI:10.1074/jbc.M800688200 · 4.60 Impact Factor
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ABSTRACT: The laminin G-like (LG) domains of laminin-111, a glycoprotein widely expressed during embryogenesis, provide cell anchoring and receptor binding sites that are involved in basement membrane assembly and cell signaling. We now report the crystal structure of the laminin alpha1LG4-5 domains and provide a mutational analysis of heparin, alpha-dystroglycan, and galactosylsulfatide binding. The two domains of alpha1LG4-5 are arranged in a V-shaped fashion similar to that observed with laminin alpha2 LG4-5 but with a substantially different interdomain angle. Recombinant alpha1LG4-5 binding to heparin, alpha-dystroglycan, and sulfatides was dependent upon both shared and unique contributions from basic residues distributed in several clusters on the surface of LG4. For heparin, the greatest contribution was detected from two clusters, 2719RKR and 2791KRK. Binding to alpha-dystroglycan was particularly dependent on basic residues within 2719RKR, 2831RAR, and 2858KDR. Binding to galactosylsulfatide was most affected by mutations in 2831RAR and 2766KGRTK but not in 2719RKR. The combined analysis of structure and activities reveal differences in LG domain interactions that should enable dissection of biological roles of different laminin ligands.Journal of Biological Chemistry 05/2007; 282(15):11573-81. DOI:10.1074/jbc.M610657200 · 4.60 Impact Factor
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ABSTRACT: Slit is a large secreted protein that provides important guidance cues in the developing nervous system and in other organs. Signaling by Slit requires two receptors, Robo transmembrane proteins and heparan sulfate (HS) proteoglycans. How HS controls Slit-Robo signaling is unclear. Here we show that the second leucine-rich repeat domain (D2) of Slit, which mediates binding to Robo receptors, also contains a functionally important binding site for heparin, a highly sulfated variant of HS. Heparin markedly enhances the affinity of the Slit-Robo interaction in a solid-phase binding assay. Analytical gel filtration chromatography demonstrates that Slit D2 associates with a soluble Robo fragment and a heparin-derived oligosaccharide to form a ternary complex. Retinal growth cone collapse triggered by Slit D2 requires cell surface HS or exogenously added heparin. Mutation of conserved basic residues in the C-terminal cap region of Slit D2 reduces heparin binding and abolishes biological activity. We conclude that heparin/HS is an integral component of the minimal Slit-Robo signaling complex and serves to stabilize the relatively weak Slit-Robo interaction.Journal of Biological Chemistry 01/2007; 281(51):39693-8. DOI:10.1074/jbc.M609384200 · 4.60 Impact Factor
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Imperial College London
London, ENG, United Kingdom
- • Department of Life Sciences
- • Division of Cell and Molecular Biology
University of Wisconsin–MadisonMadison, Wisconsin, United States
University of Cambridge
Cambridge, England, United Kingdom
- Department of Physiology, Development and Neuroscience