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Birdie on first playoff hole secures title As the final threesome in the Minnesota State Open approached the tee on what was supposed to be the 54th and final hole of their stay in Brooklyn Park, none of the three participants had identical scores. But by the time the flag was returned to the hole [...Read More
Aug. 18 benefit to help raise funds for battle with ALS It was just last November when Gavin Pugh was fighting to help get his Champlin Park High School boys soccer team into what would be his second state tournament appearance as the Rebels head coach. The bout would go his way, as the Rebels [...]...Read More
Legion baseball tournament held at Wintercrest Had the weather held and the scheduled played out as it was intended to, the Sub-State 9 American Legion baseball champion would have been crowned July 27. As it was, and baseball players in these parts are used to this following the spring season they ...Read More
Junior Camps in its fourth decade of teaching The gallery was of their own kind, wide-eyed and excited about the shot in front of young Mik Cottingham. The five-year old ignored the onlookers long enough to hear one last bit of instruction from a teacher a few paces off to his side, before coming ...Read More
Team happy with 3-2 loss to eventual U16 champions The preparation would begin as soon as the scheduled was released, and it nearly paid off with what would have arguably been the biggest upset of the USA Cup. The MapleBrook Under-16 girls team playing in the Gold Division tournament was handed the ...Read More
The Rebels Soccer Club made their official entrance into the 2013 USA Cup during the July 16 Opening Ceremonies at the National Sports Center in Blaine. The tournament, which ran from July 12 through July 20, drew over 1,000 teams to Blaine this summer. (Sun staff photo by Jason Olson)...Read More
Legion team off to 13-3 start The tractor had come to rest near third base after Champlin Park American Legion baseball coach Mike Loberg had finished dragging a field his team had just used to push its record a full 10 games over the .500 mark. Frankly, there wasn't much work that needed to be [....Read More
I first met Janet Benz seven years ago. Her heart was crushed, but she was intent on picking up the pieces and turning them into something good. At the time, her loss was one that none of us can truly fathom. Even now, we still can't. Benz's son Christopher had decided to take his own [...]...Read More


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Juntao Lai wins best paper by a young researcher at GISRUK 2015
Published: Apr 20, 2015 4:39:32 PM
SpaceTimeLab research top of the pops!
Published: Jan 26, 2015 4:30:53 PM
SpaceTimeLab for Big Data Analytics Newsletter 2014
Published: Jan 6, 2015 4:06:54 PM
Tomoki Nakaya
Professor Tomoki Nakaya's (Ritsumeikan University) research interests include spatial statistics, geographical mathematical modelling, geovisualiastion and analytical health geography.
for more information on Prof. Nakaya's research, please see the follwing link:
Page last modified on 25 feb 14 16:34

subset: commoncrawl


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I want to start by saying that I am sorry for a lack of posts. I have been very busy with real life stuff and wildly unmotivated to write anything down.
I do have two more sessions of WitchCraft to write up and a bunch of Gaming with Kids… but you know, I am lazy and all of that. Hopefully soon I will get in gear and start posting again regularly.
Again sorry for my sloth.


text:
Originally written on Fox Sports Arizona | Last updated 1/18/13
TEMPE, Ariz. Jahii Carson has seen Nick Johnson do some pretty amazing things. "Eight, nine years old, touching backboard and stuff," Carson recalled. Johnson still has the hops and Carson the ball-handling skills that they began to develop as teammates with the Arizona Stars, a Phoenix-area AAU team. More than a dozen years later, the two will oppose each other for the first time in college when Johnson and No. 7 Arizona (15-1) meet Carson and Arizona State (14-3) on Saturday afternoon. "He was always cool. He was always athletic. Just crazy the things he could do. Its exciting for me to see how good he is doing and how well he is holding his composure being on his team," Carson said as he prepared for his first intrastate rivalry game. Born less than four months apart, Carson and Johnson were almost inseparable on the Phoenix basketball circuit, linked by their standout talent. They played on the same team in summer ball. They attended the same camps. They played on all-star ...
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Is there an easy way to force the iPhone to take a photo in landscape and not allow portrait orientation? The app will be in portrait, but when the camera launches, I'd like it to be in landscape and not allow the user to take a portrait photo.
Take the 2-minute tour ×
Stack Overflow is a question and answer site for professional and enthusiast programmers. It's 100% free, no registration required.
I dont think you can really do this; you could try to place an overlay on the UIWindow that tells the user to hold their phone in landscape mode. Then remove the view if the user holds their phone the correct way, and vice versa.
Just found a simple way (tested on iOS 6). With this code, the interface will never rotate to portrait, only landscape. Thus:
you're sure to have a landscape picture
the interface clearly shows to the user that it's landscape only
Sample code:


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24 years of experience
Video profile
Accepting new patients
24 years of experience
Video profile
Accepting new patients
420 NE Glen Oak Ave
Ste 201
Peoria, IL 61603 Phone number & directions
Ste 201
Peoria, IL 61603 Phone number & directions
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A medical license is required for a doctor to practice medicine in a particular state. Requirements vary by state but most require, at a minimum, post-graduate training in the doctor's specific specialty. An NPI number is a national identifier unique to an individual health care provider and is managed by the Center for Medicare & Medicaid Services (CMS).
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Dr. Mary Schultz, MD is board certified in Pediatrics.
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Sam Gratton
May 3, 2013
The University of Montana's Sam Gratton, Josh Harris, and Peter Nguyen were named to the National Football Foundation's Hampshire Honor Society, that organization announced recently (Thursday, May 2).
The NFF recognized 703 seniors from across all divisions of college football for academic achievement. To qualify for the team a player must have completed his eligibility with a cumulative grade point average of 3.20 or better, and be a starter or key reserve.
A wide receiver from Billings, Gratton started all 11 games this past season and had a team-high (tie) 29 receptions and led the Griz with four touchdown grabs. He was an honorable mention All-Big Sky Conference pick.
Gratton ended his career ranked 26th in school history with 1,423 career receiving yards. He was the 2012 co-recipient (with senior tight end Greg Hardy) of Montana's Terry Dillon Award (outstanding back or receiver). Gratton maintains a 3.48 in business management.
Harris, a defensive end from Kalispell, started in 10 of 11 games last year, and was UM's ninth leading tackler with 39 stops. He was third on the team with 7.5 tackles for loss, and led UM with six quarterback hurries. Following his senior season he received UM's Pat Norwood Award (most inspirational player).
Harris was also named first team on the College Sports Information Director Association's (CoSIDA) Capital One Division 1 Academic All-District 7 football team, and carries a 3.67 GPA in sociology.
A running back and punt returner from Seattle, Wash., Nguyen (pronounced win) played in all 11 games a year ago and led the Griz with 926 yards rushing, which ranked him sixth in the Big Sky. This past season he was chosen UM's Steve Carlson Award (MVP) winner, and was a second team all-league pick. He was ranked 18th in the FCS in punt returns (9.88 average).
Nguyen finished his career at Montana ranked sixth in school history with 1,985 career rushing yards, and all purpose yards with 4,323. He has a 3.27 GPA in business management.
The Grizzlies had three of the 14 players from the other Big Sky football-playing schools, who were named to the squad.
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33 Welch Dr
Forestville, CT 06010-0601
Forestville, CT 06010-0601
Tel: (860)583-5214
St. Matthew School is a small private Catholic school located in Forestville, Connecticut and serves the members of St. Matthew parish, local parishes and the surrounding communities.
As God's covenant people, we fulfill the religious and academic purpose of Catholic education to teach Catholic doctrine and moral values, to develop academic skills, to create a high standard of academic achievement, to build a positive self image and respect for others.
The St. Matthew School Development Program was established in 1990 and is largely a volunteer, school board driven, comprehensive process, which strives to develop friends, students, and funds.
The Development Program's purpose is essentially threefold to build awareness and acceptance for St.Matthew School, To seek and retain students for the school.
St. Matthew School is committed to the education of the whole child by recognizing and accepting each individual regardless of age, gender, creed, socio-economic status or race as a unique creation loved by God.
As a faith community, priests, sisters, lay faculty, staff, parents, parishioners and students are committed to impart the Gospel as a way of life by striving to actively live and spread this Christian message.
As we work together to educate students spiritually, academically, and socially we prepare them to become contributing Christian members of society.
It is our responsibility to follow the policies and guidelines of the Archdiocese of Hartford and therefore provide challenges and opportunities for all students to experience growth in all aspects of their development.
School Overview
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|265 students
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State avg.: 24%
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National avg.: 13:1
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PlainvilleGreat Beginnings
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335 Center St
Bristol, CT 06010
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1001 Stafford Ave
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(860)584-0911(Roman Catholic)2 mi | 439 students | Gr. 9-12
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When it comes to choosing a private school, there are so many choices to consider that the project can be a huge challenge to most of us. We offer some help and advice to help you get started.
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|Instructor Info:
|Suzette Spencer
|Meeting Info:
|"Formations of Black Subjection and Scenes of Resistance" departs from Saidiya Hartman's book Scenes of Subjection in its exploration, identification, and interrogation of formations of black subjection and resistance to subjection from the eighteenth century to the present. We will perform close readings of literature, law, and various cultural texts, forms of sociality, and spatial arrangements by and through which racialized, gendered, and socio-economic class-based forms of subjection and coercion have been constructed and are in some instances still maintained. We will engage the problem of resistance-its wily definitions and expressions, conditions of possibility, potentiality, and limits. And we will look in both common and unexpected places, mining how discourses of liberty, freedom, egalitarianism, resistance, and even empathy, humanitarianism, inclusiveness, and joy can become leveraged strategically as vehicles for subjection and problematic exclusions. Requirements: presentations, research- papers, independent projects, attendance.
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Found 3 Items Tagged "stallion"
The engraving on this shell depicts a falcon warrior wearing a beaked mask, a cloak of feathers, and ear spools. Vomit or a speech scroll em...
Hiroshige's One Hundred Famous Views of Edo
Hiroshige's 118 woodblock landscape and genre scenes of mid-nineteenth-century Tokyo, is one of the greatest achievements of Japanese art.
Metjetji, the subject of this statue, is shown in later life, with the long kilt of a senior official and, as viewed from the side, a rather...
200 Eastern Parkway
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Eastern Parkway/Brooklyn Museum
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Oregonian/Oregonlive Saturday - 2015 Intel Oregon International Air Show
- Auto, Boat & Air
- 3355 NE Cornell Rd, Hillsboro, OR, 97124
Telehealth: Fact or Fantasy
Clark County Commission on Aging
Wednesday, October 2, 2013 from 7:30 AM to 12:00 PM (PDT)
Telehealth has the potential to improve the delivery of healthcare in a variety of ways. This includes playing a role in helping seniors age in place and live independently as long as possible.
This conference is organized by the Clark County Commission on Aging. It is the first of its kind in our community. Its aim is to provide the opportunity for the articulation and discussion of this emerging technology and how it can enhance the health and well-being of our citizens.
There is no cost to attend. However, if you plan to attend, your registration would be appreciated.
Check in and a light continental breakfast will begin at 7:30 a.m. The conference will start promptly at 8:00 a.m.
We hope to see you at this important event.
As community ambassadors, the Commission on Aging provides leadership, advocacy, community awareness, and develops partnerships to initiate positive change toward an all-age friendly, livable community.
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on ArtFire
on ArtFire
This shop is currently closed. Please visit my profile.
Welcome to CoconutroadSouth
I sell vintage, retro, antique, collectible and unusual treasures from the past.I particularly love vintage from the 1950s and 60s. Lately, I've been finding lots from the Danish Modern era. And, every once in a while I'll find an original "skinny tie". Check out both my vintage shops (etsy, too) to see what I've come across lately! Thanks for coming by.Joyce More »


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Fax: (519) 767-1081
In situ heparin-induced peroxisomal reticulum and biogenesis of peroxisomes in pulmonary intravascular macrophages (PIMs) of caprine lung: an ultrastructural and cytochemical study
Article first published online: 7 DEC 2001
DOI: 10.1002/ar.10035
Copyright © 2002 Wiley-Liss, Inc.
The Anatomical Record
Volume 266, Issue 1, pages 69–80, 1 January 2002
Additional Information
How to Cite
Atwal, O. S., Williams, C. S., Minhas, K. J. and Nijjar, M. S. (2002), In situ heparin-induced peroxisomal reticulum and biogenesis of peroxisomes in pulmonary intravascular macrophages (PIMs) of caprine lung: an ultrastructural and cytochemical study. Anat. Rec., 266: 69–80. doi: 10.1002/ar.10035
Publication History
- Issue published online: 7 DEC 2001
- Article first published online: 7 DEC 2001
- Manuscript Accepted: 9 AUG 2001
- Manuscript Received: 2 MAY 2001
Funded by
- National Scientific and Engineering Research Council of Canada
- Ontario Ministry of Agriculture and Food
- Abstract
- Article
- References
- Cited By
- lipolytic lipase;
- free fatty acids;
- receptors;
- catalase;
- nutrition
Pulmonary intravascular macrophages (PIMs) contain a unique electron-dense globular surface-coat which is sensitive to heparin treatment, halothane anesthesia, and the digestive effect of lipolytic lipase (LPL), suggesting that the coat is predominantly composed of lipoproteins. In the present study, evidence is presented that heparin, when administered intravenously in goats, potentiated both the translocation of the surface-coat into the vacuolar system and the expansion of the Golgi apparatus. Sequentially, these changes were followed by proliferation of peroxisomes in combination with peroxisomal reticulum (PR), a transient precursor of this organelle. The peroxisomes, as well as PR, reacted positively for catalase after aldehyde fixation and 3,3′-diaminobenzidine (DAB) staining. In addition to their role as phagocytes, the ultrastructural and cytochemical detection of peroxisomes suggests a functional capacity of the PIMs, which may be adaptable to the circulating level of free fatty acids (FAAs). Anat Rec 266:69–80, 2002. © 2002 Wiley-Liss, Inc.
Pulmonary intravascular macrophages (PIMs) are one of the recently recognized representatives of the mononuclear phagocyte system (MPS), a lineage of cells derived from the monoblastic series in the bone marrow (Winkler, 1989; Warner and Brain, 1986; Atwal and Saldanha, 1985). They are most abundantly seen in the lung parenchyma, by virtue of their habitat in the pulmonary microvessels of several species of animals (Atwal et al., 1989; Winkler, 1988). They are recognized by the specific surface-coat and adhesive junctions they form with the pulmonary endothelium, particularly in goat, sheep, cattle, and horse. The surface-coat consists of linearly arranged globular units along the external leaf of the cell membrane, in close conformity to the outer contours of the cell surface. Several experiments have provided indirect evidence that the coat-globules are predominantly composed of lipoproteins (Atwal et al., 1989; Singh et al., 1995a, b; Atwal et al., 1994; Jassal, 1989; Atwal and Minhas, 1992). The linearly-arranged coat-globules are not membrane-bound, but are separated by a translucent gap (the lamina lucida), approximately 35–39 nm wide, from the outer leaflet of the cell membrane.
In general, PIMs satisfy the major functional criterion of marked phagocytosis (Atwal et al., 1992b, c). The phagocytosis of exogenous tracer substance is critically dependent on the integrity of the globular surface-coat (Atwal and Minhas, 1992). Once the surface-coat is washed away by physiological detergents, there is a drastic reduction in the pulmonary clearance of exogenous material injected via the venous channels (Longworth et al., 1996). The PIMs also localize circulating pathogens and concentrate the inflammatory response as putative "hot spots" on the vascular side of the air–blood barrier (Warner, 1996).
In previous studies we have shown that the surface-coat of PIMs in sheep, goat, and cattle is sensitive to a single intravenous heparin treatment as well as to the digestive effect of lipolytic lipase (LPL) in vitro. In concert with heparin-induced translocation of the coat, the plasma levels of oleic, palmitic, and stearic acid were also elevated. After its initial disappearance, the coat is reconstituted 4–8 hr after a single treatment of heparin (Atwal et al., 1989; Atwal et al., 1992a; Singh et al., 1995a). The ultrastructural disappearance of the coat from the surface of the PIMs, 30 min after i.v. injection of heparin, was attributed to the lytic effect of LPL on the globules of the coat. Hormones, cytokines, and heparin are known to release LPL not only from the extracellular matrix of the endothelial cells, but also from monocyte-derived macrophages in tissue culture. Lipolytic lipase (LPL) is also the rate-limiting enzyme in the hydrolysis of serum triglycerides, derived from low-density lipoproteins (LDLs) (Nielsen et al., 1997; Knuston, 2000; Querfeld et al., 1990).
In the present study, we examined the disappearance of the surface-coat of the PIMs in the goat lung after treatment with heparin. Evidence is presented that, in parallel with the disappearance of the coat, there was some enhanced translocation of the globular units of the coat into the vacuolar system of the PIMs. In addition, the Golgi apparatus was seen expanded 2 hr after the heparin administration. Thereafter, the induction of peroxisomes and allied cell organelles took place 4–8 hr after heparin treatment. The peroxisomes reacted positively for catalase after aldehyde fixation and 3,3′-diaminobenzidine (DAB) staining. The DAB technique for localization of catalase, a marker of peroxisomes, is still the cytochemical method of choice in the initial morphological survey for the identification of this organelle (Fahimi and Baumgart, 2000). We also analyzed the formation of peroxisomal reticulum (PR), a transitional organelle thought to be involved in the early stages of de novo formation of peroxisomes (Yamamoto and Fahimi, 1987; Lazarow and Fujiki, 1985).
Clinically healthy goats (five male and eight female, 6 months to 1 year old (average 9 months)) were used in this study. Goats were purchased from local breeding farms. All animals were acclimatized to the controlled, isolated housing conditions for 1–2 weeks; animals were dewormed, stall fed, and allowed free access to water.
Heparin sodium injection USP was purchased from Allen and Hamburys Co. (Toronto, Canada).
Treatment Protocol
Heparin was injected intravenously at a dose rate of 50 U/g/kg of body weight in the jugular vein of 11 goats. Periods of 30 min (two animals), 2 hr (three animals), 4 hr (three animals), and 8 hr (three animals) were allowed between the injection and killing of the animals. Two animals were injected with normal saline and were used as controls. All animals were euthanized with an overdose of barbiturate.
Immediately after euthanasia the animals were positioned in sternal recumbancy and the lungs were fixed by intratracheal instillation of 500–800 ml of fixative (2% glutaraldehyde and 2% paraformaldehyde in 0.2 M HCl-Na cacodylate buffer, pH 7.4) to an inflation pressure of 30 cm H2O to ensure adequate unfolding of the alveolar wall. The animals were put into a large cooler where fixation was carried out for 30 min for routine electron microscopy and cytochemistry for catalase. After fixation in situ, blocks of tissue were collected from the cranial, middle, and caudal lobes of the right lung. Small strips of tissue were further fixed in the same fixative for 1 hr. Following fixation, the lung tissue was rinsed in the same buffer and cut on a tissue sectioner into 30-μm sections, which were immediately incubated.
Incubation for Catalase
The incubation medium, with minor modifications, was prepared according to the method described by Yamamoto and Fahimi (1987). The medium contained 1 mg/ml of 3,3′-diaminobenzidine tetrachloride (Sigma Chemical Company, St. Louis, MO) prepared in cacodylate buffer at pH 7.4 and 0.02% H2O2. The incubation was carried out at room temperature for 1 hr. For the cytochemical control preparation, we omitted the peroxide substrate in the incubation medium, or boiled the sections for 5 min in glutaraldehyde before incubation (Deimann et al., 1984).
Fixation and Tissue Preparation for Electron Microscopy
After fixation in situ, specimens for routine electron microscopy collected from the cranial and caudal lobes of the left lung and the cranial, middle, and caudal lobes of the right lung were diced into small pieces of about 1 mm3, and fixation was continued by immersion in the same fixative for 1–2 hr.
All tissues taken from all lobes were post-fixed for 90 min in 1.5% OsO4 in 0.1 M HCl-Na-cacodylate buffer (pH 7.6). Tissues for routine electron microscopy were stained en bloc with 0.5% tannic acid in 0.1 M-HCl-Na-cacodylate buffer for 30 min at room temperature.
All tissues thus prepared were dehydrated in ethanol and propylene oxide, and were finally embedded in Jembed 812 (J.B. EM Services Inc.). Thick sections were stained with toluidine blue–basic fuchsin and were viewed in a Zeiss universal microscope. Ultrathin sections for routine electron microscopy were stained with uranyl acetate and lead citrate, whereas ultrathin sections of tissue for cytochemistry (for catalase) were not exposed to double differentiation. All sections thus prepared were examined with a JEOL-100S electron microscope at 80 kV.
Ultrastructure of PIMs, Disposition of the Surface-Coat, and Its Relationship With the Surface-Structure and the Vacuolar System
The ultrastructure of goat PIMs is described in detail in our previous studies (Atwal et al., 1989; Atwal et al., 1992; Atwal et al., 1992c), especially the unique morphology of the globular surface-coat and its interrelationship with the plasma membrane and its surface structures. However, a few salient features of this special organelle, which are central to the experimental data of this study, following its manipulation with heparin are presented in Figs. 1 and 2. The surface-coat, as usual, is seen arranged into a linear chain of globules. The globules, after fixation with tannic acid and paraformaldehyde-glutaraldehyde fixatives, are highly electron-dense and reveal a characteristic periodicity, created by the intervening translucent space between the globular-units of the coat. The coat is not a static extracellular structure but maintains a dynamic relationship with the plasma membrane, across which constitutive and receptor-mediated endocytosis of the coat-globules into the vacuolar system takes place. This special topology of the coat inside and outside the PIMs underscores a sensitive homeostatic balance between the extracellular (vascular) and intracellular microenvironment of the PIMs. A few mitochondria, microbodies, ER, and a modestly defined Golgi apparatus are present, as with any other macrophage of the MPS (Figs. 1 and 2).
Heparin-Induced Alterations in the Ultrastructure of the PIMs and Its Surface-Coat
Complete absence of the coat from the surface, and cell surface changes.
By 30 min to 2 hr post i.v injection, the globular surface-coat had completely disappeared from the surface (Figs. 3–6). A few globules of the coat were seen internalized in the endocytotic system. The individual globular units of the coat were seen discretely intact in some endosomes. Three or four well-defined surface invaginations, as "omega"-shaped clathrin-coated pits, were an important manifestation of heparin-induced alterations in the plasma membrane and the surface-coat. The individual coated pits maintained an intimate one-to-one relationship with the globules, an essential condition for the initial step prior to receptor-mediated endocytosis. An occasional peroxisome with a well defined matrix, and surrounded by a single membrane, was observed at this time in heparin-treated goats. Cleft-like dilations of ER and Golgi cisternae, and an electron-lucent interior were indicative of crystallization of excess saturated fatty acids or triglycerides (Hawley and Gordon, 1976). A uniform distribution of monoribosomes throughout the cytosol was a consistent feature of the heparin-exposed PIMs (Figs. 3–7).
Loss of the surface-coat coupled with expansion of the Golgi complex 2 hr post i.v. treatment with heparin.
Concurrently with the coat loss, the PIMs enlarged their area of adhesive contact with endothelial cells of the capillaries. In addition, the expansion of the Golgi apparatus, especially the trans-Golgi network (TGN), was also visualized. The TGN was profusely enriched with small coated vesicles as well as with prominent tracts of microtubular bundles radiating from the TGN in various directions, underlying active trafficking to and from the plasma membrane of the PIMs (Figs. 4 and 5).
Cell spreading, lamellipodial formation, nuclear pores, and microtubular network assembly.
In addition to the disappearance of the coat from the surface, the plasma membrane of the PIMs was thrown into complex arrays of lamellipodial and microvillous projections (Fig. 3). The microtubular assembly around the nuclear membrane and the Golgi apparatus was consistently seen as an early effect of the heparin treatment. The anchorage of microtubules to the nuclear envelope and open nuclear pores perhaps signified more extensive communications with the cytoplasm as well as nuclear volume changes during cell spreading (Figs. 5–8).
Dramatic appearance of large membrane-bound structures resembling PR, and vesicular buds arising from PR 4–8 hr post-heparin treatment.
Ultrastructural profiles of irregular, interconnected single membrane-bound structures appeared in the cytosol of PIMs 4–8 hr after i.v. injection of heparin in the goats (Figs. 7–9). We classified these structures as PR. Examination of unstained and DAB-stained sections for catalase strongly suggested that these structures were PR, although the interconnections were not the typically narrow hourglass bridges characterized by Yamamoto and Fahimi (1987). The use of the DAB technique revealed strong localization of catalase exclusively in the PR (Fig. 9), microperoxisomes, and peroxisomes (Figs. 9–12).
The ultrastructural features, such as the pleomorphic internum of PR, divided into two distinct zones of variable electron density after heavy metal staining, and were observed in most of the samples examined with the transmission electron microscopy (TEM) (Figs. 7–9). Coupled with this dichotomy, several small vesicles detached directly from the limiting membrane of the PR as buds which, through fission and fusion, appeared to develop into peroxisomal bodies of spherical, spherical with tail, tubular, and curved lenticular subforms (Figs. 7 and 8). The PR, as well as these subforms, were positively stained with the DAB technique for catalase (Figs. 9–12), although we could not ascertain a direct connection of subforms of the peroxisomal vesicles with PR during cytochemistry for catalase. The multiforms of DAB-positive bodies and their interactions with PR may represent local remodeling in order to develop, through a process of fusion and fission, into microperoxisomes and definitive peroxisomes. Taken together, these images may represent de novo biogenesis of peroxisomal structures. Therefore, microperoxisomes from small vesicles to spherical, tubular, and lenticular subforms may well be a model for the sequential multistep assembly pathway (Titorenko and Rachubinski, 1998; Titorenko et al., 2000; Bentfield et al., 1977) initiated at the PR, a transiently formed reticulum (Schrader et al., 2000).
Reconstitution of the surface-coat and regression of the Golgi apparatus 4–8 hr post i.v. heparin treatment.
After the initial disappearance of the surface-coat and the translocation of some of its globular units into the vacuolar system, the reconstitution of the coat began at 2 hr post-heparin treatment. The complete reinstatement of the coat took place by 8 hr of i.v. heparin. By this time, the Golgi apparatus did not remain as conspicuously prominent as it was at 2 hr post-heparin treatment, although the ribosomes, ER, and microtubules remained prominent in the cytoplasm to cope with differentiation of the peroxisomal structures (Figs. 9–12).
Mature and definitive peroxisomes and their spatial relationship with ER.
Examination of ultrathin sections fixed with paraformaldehyde-glutaraldehyde and incubated in DAB-medium without H2O2 or boiled in glutaraldehyde before incubation did not stain for catalase, but revealed discrete, single, membrane-bound, fully differentiated peroxisomes in the PIMs at the time when globular surface-coat was partially reconstituted, 8 hr after a single injection of heparin in the goats (Figs. 11 and 13). Most of the peroxisomes appeared in close spatial association with active ER. The individual ER cisternae studded with ribosomes frequently curled around the peroxisomal particles in close apposition to their limiting membrane. The peroxisomes, unlike the peroxisomes of hepatic cells, did not show a marginal plate or a crystalline nucleoid core. However, they presented a complex pleomorphic internum studded with electron-dense tubular structures and electron-dense bodies of variable size and electron density (Figs. 11–13).
DAB staining of untreated PIMs, neutrophils (PMN), and platelets (positive control).
After staining with DAB and H2O2 in the incubation medium, we noted a strong expression of catalase in the azurophilic granules of the PMN and dense bodies of the platelets (Bentfield et al., 1977) (Fig. 14). A completely covered PIM with a full complement of its globular surface-coat did not express catalase in any of its endosomal compartment or in any other compartment of the cell (Fig. 15).
We have presented ultrastructural and cytochemical evidence that a single intravenous injection of heparin removed the globular surface-coat and potentiated its partial entry into the vacuolar system of PIMs. Following intracellular translocation of the coat, probably via receptor-mediated endocytosis, the TGN compartment of the Golgi complex expanded as an obligatory response to the intracellular entry of the coat material. In temporal succession to these changes, proliferation of peroxisomes and a dramatic appearance of PR in the PIMs was also seen concurrently with the expansion of ER, which primarily remained closely related to the newly arrived peroxisomes (Yamamoto and Fahimi, 1987; Erdmann et al., 1997). The validity of peroxisomes was confirmed by using the DAB technique for catalase, which is the marker enzyme of peroxisomes. According to Fahimi and Baumgart (2000), the DAB technique for catalase localization is still the most commonly used cytochemical method for the initial morphologic survey to identify this organelle.
In a previous study (Atwal et al., 1992a) we showed that intravenous heparin triggered higher levels of oleic, palmitic, and stearic acids in the plasma. This biochemical change was attributed to the lytic effect of LPL, mobilized from the extracellular matrix of the pulmonary endothelium and/or secreted by the PIMs (Querfeld et al., 1990; Hamosh and Hamosh, 1985; McCrohon et al., 1999). The LPL has an alkaline pH optimum, is activated by a low concentration of heparin, and provides the only way to channel FFAs to the tissues whenever they are needed. The enzyme hydrolyzes the Sn-1 or Sn-3 and Sn-1 ester bonds of triglycerides and phospholipids of lipoproteins, respectively (Hamosh and Hamosh, 1985). The intracellular translocation of the coat-globules can best be explained by assuming that LPL/LDL complexes were internalized by the PIMs upon binding to plasma membrane-associated heparin sulfate, an interaction which is heparin-sensitive under physiological conditions (Schönherr et al., 2000).
Some of the earliest ultrastructural changes 2 hr after intravenous heparin were the expansion of the Golgi apparatus and the proliferation of polyribosomes, monoribosomes, ER, and mitochondria, in combination with the microtubular network. The FFAs play an important role in cell homeostasis by serving as a metabolic energy source and building blocks for membrane lipids, thus regulating various cell functions (Stewart, 2000). Increasing evidence suggests that plasma membrane-associated and cytoplasmic fatty acid binding proteins are involved in the uptake and intracellular transport system of FFAs (Dutta-Roy, 2000; Stewart, 2000; Kennedy, 2000).
The present initial cytoarchitectural changes perhaps underscore the requirement of an upregulated synthesis of binding and cellular transport proteins for the uptake of elevated plasma FFAs by the PIMs (Luby-Phelps and Weisiger, 1996; Weisiger, 1996; Pelham, 1997). These diverse manifestations of nutritional, metabolic, and functional roles of FFAs in the mammalian system depend upon their role in specific targeting of cell organelles, such as the mitochondria, endoplasmic reticulum (McArthur et al., 1999; Ribarik et al., 1999), and Golgi apparatus (Gonatas et al., 1992; Rothman and Orci, 1992; Atwal et al., 1994). The Golgi apparatus serves as a direct pathway for diffuse exchange of lipids. The intermixing (lateral diffusion) of lipid molecules is 10 times faster than that of proteins to reach a maximum intracellular concentration at the Golgi level (VanMeer, 1989; Montgomery and Cohn, 1989; Orci et al., 1997). The conspicuous prominence of the Golgi apparatus (especially the TGN), possibly to an influx of FFAs, 2 hr post-heparin treatment was perhaps an early response, as a relatively resistant cell component for synthesis, metabolism, and membrane trafficking—activities for which the Golgi apparatus appears to be quite labile (Rothman and Orci, 1992; Bannykh et al., 1998; Simons and Ikonen, 2000). It is thought that the attachment of nuclear membrane to microtubular tracts in the cytosol accounts for the nuclear volume changes which occur during cell spreading. Furthermore, mechanical expansion of the nucleus as a result of spreading is thought to open nuclear pores (Luby-Phelps and Weisiger, 1996) necessary for augmented communication with the cytoplasm for anchorage-dependent cell function, particularly cytoskeleton-mediated transport between the nuclear envelope and the Golgi apparatus (Farquhar and Hauri, 1997; Tanaka and Noguchi, 2000).
To our knowledge, the present study demonstrates the first morphologic detection of peroxisomes in the PIMs, which characteristically possess a surface-coat predominantly composed of LDL in goat, sheep, cattle, and horse (Atwal et al., 1994; Atwal and Minhas, 1992; Singh et al., 1995a). The peroxisome is a recognized cell organelle of lipid metabolism (Aboushadi et al., 1992; van den Bosch et al., 1992). In the present model of lipid mobilization, after manipulation with heparin, we also present the induction of a structure reminiscent of PR, a transient precursor structure involved in the biogenesis of peroxisomes (Lazarow and Fujiki, 1985). The PR and peroxisomes were observed in juxtaposition as well as in direct connection with each other, and were exclusively stained for catalase.
In a recent study of HepG2 and Cos-7 cells, images of the dynamic behavior of a variety of morphologically different peroxisomal structures were observed (Schrader et al., 2000). The real-time imaging revealed that moving peroxisomes made transient contacts to form PR. According to these authors, peroxisomal behavior in vivo is significantly more dynamic and interactive than was previously thought (Subramani, 1998). We have observed heterogenous populations of peroxisomal structures and their mutual interactive contacts, although the present interpretation is based on examination of static electron microscopic views of fixed lung tissue. The fusion and fission of microperoxisomes suggest that peroxisome proliferation in the PIMs did take place, and that PR is a transient structure as a relevant source of peroxisome biogenesis (Schrader et al., 2000; Titorenko and Rachubinski, 1998).
There are a number of xenobiotic compounds, hypolipidimic drugs, and eicosanoids that induce peroxisome proliferation (Reddy and Mannaerts, 1994; Reddy and Chu, 1996; Lemberger et al., 1996; Krey et al., 1997). In recent years, among the many inducers of peroxisomal proliferation, FFAs are also recognized as natural inducers. The FFAs, as major dietary constituents, are capable of regulating gene expression in response to food intake and qualitative nutritional changes. The induction of peroxisomes is dependent upon the transcription of responsive genes through the binding of peroxisome proliferator activated receptors (PPARs) (Gearing et al., 1994), which have emerged as one of the central regulators of nutrient/gene interactions.
The early Golgi expansion at 2 hr post i.v. heparin was seen as a cellular requirement of membrane trafficking during cell spreading. It may have acted as a stable compartment of protein (glycoprotein) synthesis for the uptake of FFAs in response to FFA influx and intracellular traffic. Subsequently, the induction of peroxisomes and allied structures was perhaps a response to sustain β-oxidation of increased concentration of FFAs and their metabolic fate, thus modulating cell growth and development via PPAR-γ, as a second stage of this biphasic response (Dutta-Roy, 2000; Krey et al., 1997).
In view of the dynamic relationship of the surface-coat with the membrane of the PIMs, the sensitivity of the surface-coat to heparin (expressed by its rapid mobilization into the cell (Atwal et al., 1992c)), and the elevated plasma levels of FFAs (Atwal et al., 1992a), the induction of peroxisomes could be an FFA-triggered, gene-related encoding of peroxisomal proteins (Gearing et al., 1994). In a recent study, Ricote and coworkers (1999) suggested that FFAs and eicosanoid metabolites activate PPAR-γ, and are involved in macrophage biology and cell cycle regulation. It has already been shown that metabolism of vasoactive lipids in the PIMs is manyfold higher than that of the alveolar macrophages (Bertram et al., 1988).
In addition to their role as phagocytes in the defense against lung disease (Warner, 1996), the present fine structural and cytochemical tests for peroxisomes are suggestive of their capacity to adapt to the circulating level of FFAs in the pulmonary circulation in the goat. The peroxisomal changes in the PIMs are interesting not only in terms of regulating inflammatory disease but also in influencing diet-related lipid homeostasis in the lung. They also raise the possibility of involvement in free-radical and charge-related lung injury, in which activated PIMs may exert an important role (Chang and Voelkel, 1989; Simon et al., 1991).
Finally, the present inquiry is a preliminary one, limited in both extent and depth because of the small number of goats used at each time point. However, we attempted to increase the number of samples from the cranial, middle, and caudal lobes of the right lung at several selective sites for ultrastructural and cytochemical analyses. The changes occurred uniformly and were repeatable at each TEM scrutiny. It is evident that the present model of peroxisomal changes imparts to the PIMs an important role in influencing lipid metabolism in the lung of domestic ungulates. In future research we need to vigorously investigate heparin-induced sequential changes by examining serial sections of lung tissue in order to establish a convincing relationship between PR and peroxisomes. More sophisticated cytochemical techniques for a wider range of peroxisome-specific enzymes in combination with morphometry are available to contribute significantly to our knowledge of peroxisomes in the PIMs (Fahimi and Baumgart, 2000; Usuda et al., 1999). The ubiquity of peroxisomes was firmly established only after the systematic application of the DAB histochemical technique (Fahimi and Baumgart, 2000). The present study is just a beginning in the attempt to define the biology of heparin-induced peroxisomes. So far, in vitro studies have been handicapped because of the technical difficulties involved in the isolation and successful cell culture of PIMs. Until such time as PIMs are successfully isolated with high purity and viability for sensitive microphysiological and biochemical investigations, the present methods of fixation are still the best tools at hand for obtaining cell-specific information related to these unique cells in the lung.
We thank Ms. Shelley Rhoden, Mrs. Shana Siddique, and Ms. Cassandra Cooper for their valuable help in the computer-assisted literature survey, and Mr. David Robinson for helping in the preparation of this manuscript. Support for the Tuskegee University Imaging Facility was provided by NIH/RCMI (grant 5-G12RR03059).
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Reel World
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Idiot Box
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Inauguration around the dial
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Week in Sloth
The Week in Sloth
Today's Events
Batman (1989) at KiMo Theatre
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Tomorrow's Events
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[50] July 3, 2015 at 1:33am
So when does the revolution begin?
In reply to fedupjane's comment on the story Parents Confront School Board, Demand 'Opt-Out' for Controversial Lessons — See Why the Room Erupted at End of Meeting
[5] July 3, 2015 at 9:05am
Cosmos102 responded:
[2] July 4, 2015 at 3:05pm
AreYouFreekinKiddingMe responded:
[3] June 29, 2015 at 5:03pm
So very true… what they cannot see is that after the revolution is over, the Marxists will "listen" to the people and make homosexuality illegal once again by decree and start rounding them all up like Hitler, Stalin and Mao did. This road does not end well…
In reply to AvengerK's comment on the story The Statement That Beck Fears May Make Him 'An Exile in [His] Own Land'
[11] June 29, 2015 at 1:32pm
Having been in the company of men and women that have been there and done that, including Mr. Armstrong and Gene Cernan, I can assure you that your opinion on this matter is, well…… junk.
The Demorats in congress started crying that the moon was a waste of money and NASA started begging for a space plane capable of flying into space on a schedule like a commercial airliner. The end result is we gave up on the moon and settled for a technology that still has yet to be realized.
As good as the shuttle was, and it WAS a technological marvel, the guys that built and flew them will privately tell you the program fell well short of the goals set for the program.
[30] June 29, 2015 at 1:24pm
yep… right there in the bottom right corner, next to Obama's birth certificate, Hillary's server, and Lerner's hard drives…
I think I can make out an ISIS battle flag as well.
[43] June 23, 2015 at 4:26pm
It does not matter who is paying. We as AMERICANS will all pay the price for his incompetence for a century or more….
We strayed from Nature's Law (God) and now we shall reap what has been sewn.
[128] June 23, 2015 at 4:13pm
The new American flag will be black in color, bearing the symbol of the Islamic states of America, with th e way this administration is going. Fox is reporting that Obama has approved using money to pay for hostages release!
When can we call it treason?
I remember the oath of service, and it did state ALL enemies foreign AND domestic!
In reply to Proud2BVeteran's comment on the story 'I Have a Prediction…Do Not Doubt Me': Rush Limbaugh Claims There's More to Confederate Flag Fight
[-7] June 23, 2015 at 4:19pm
TG360 responded:
[43] June 23, 2015 at 4:26pm
johnVMFA122 responded:
[21] June 23, 2015 at 4:38pm
myrwo6 responded:
[-19] June 23, 2015 at 5:00pm
CanadianSocialist responded:
[8] June 23, 2015 at 7:55pm
fortherecord responded:
[11] June 23, 2015 at 11:10pm
pschlentz responded:
[1] June 24, 2015 at 8:14am
layne51 responded:
[1] June 24, 2015 at 11:49am
Makava responded:
[3] June 24, 2015 at 1:58pm
mcsledge responded:
[3] June 18, 2015 at 1:15am
I agree completely… And the chaos will result in barrack Osama declaring martial law and suspending our liberties. Long summer indeed.
[10] June 16, 2015 at 5:25pm
And global warming beliebers also believe Obama has a legitimate birth certificate and that dinosaurs can be cloned and brought back from the dead…
In reply to SaltwaterSerendipity's comment on the story Biden: Climate Change Deniers 'Also Deny Gravity'
[4] June 16, 2015 at 5:56pm
Paladin45ACP responded:
[10] June 16, 2015 at 10:17am
I remember having to read the book "black Like Me" in middle school in 1976 or something. I didn't think much of it then, but today I can see it for what it was…. The left has been buried deep into our education system for much longer than we give them credit.
In reply to RedDawning's comment on the story 'I Identify as Black': Ex-NAACP President Opens Up to Matt Lauer About Her 'Self-Identification With the Black Experience'
June 16, 2015 at 1:08pm
Mikev5 responded:
June 16, 2015 at 2:50pm
deeberj responded:
June 16, 2015 at 3:46pm
Lovecats responded:
[19] June 12, 2015 at 10:12am
The note was written by Jesus(Mexican pronunciation) not the biblical one, and he is right. The end is near as the illegals are now strong enough to end the American way of life in the southwest. Be vigilant and ready to defend your families and your homes.
[115] June 11, 2015 at 8:40pm
In her belly, we will find Clinton's hard drives and Obama's birth certificate.
In reply to MrSunshine's comment on the story Some Are Wondering If the Great White Shark Seen in This Viral Video Is the Largest Ever Captured on Video
[6] June 12, 2015 at 6:09am
cknapp responded:
[2] June 12, 2015 at 7:09pm
DannyG responded:
[9] June 10, 2015 at 8:45pm
I would like to be in complete agreement with Mr.Beck on this one. That being said, until Muslims are ready to clean their own house, the only thing we will have is our good will thrown in our faces by people that have been bred from birth for generations to despise anything non-muslim.
Until the might of the righteous decimates the will of Allah, I will continue to see Islam as the enemy of my liberty, and my way of life. As they are the enemy, I could care less what happens over there.
In reply to the story Glenn Beck Absolutely Unleashes on Feminists Over Middle East Hypocrisy
June 11, 2015 at 10:55am
G-WHIZ responded:
[44] June 6, 2015 at 1:29pm
And to think he could have asked God and then listened with his heart for a reply… I did and have been blessed beyond measure, and it didn't cost me a dime.
In reply to the story NYC Man Somehow Spends His Entire Wealth on Psychic in Quest for Love
[20] June 6, 2015 at 1:38pm
Jinxe responded:
[12] June 6, 2015 at 3:23pm
myrwo6 responded:
June 6, 2015 at 4:38pm
SquareHead responded:
[1] June 6, 2015 at 5:33pm
Conkuur responded:
[6] June 6, 2015 at 5:39pm
db321 responded:
[6] June 6, 2015 at 9:45pm
makingout responded:
[3] June 6, 2015 at 11:41pm
Gorp responded:
[6] June 6, 2015 at 1:05am
I hope the train was carrying lots of jihadists on their way to a mosque…
In reply to the story Amtrak Train Slams Into Semi Carrying Thousands of Pounds of Bacon
[16] June 4, 2015 at 6:08pm
Dead people and illegals have no voting rights. everyone else has an ID, and they should be required to show it.
[20] June 4, 2015 at 6:07pm
I could see through the Dem's BS by the age of 12, much to the dismay of my flower-child mother. How stupid are the sheeple that fall for this crap?
In reply to the story Hillary Clinton Says Republicans Are Waging a 'Crusade' Against Voting Rights
[23] June 4, 2015 at 6:03pm
I remember spinning records backwards for the messages…
In reply to ipockpa's comment on the story Nobody Thought Twice About These Pittsburgh Bus Ads Until One Woman Noticed the Message They Spell Backward
[1] June 4, 2015 at 6:07pm
ibbolton responded:
[3] June 4, 2015 at 8:48pm
devan95 responded:
[3] June 5, 2015 at 3:22pm
joeontheright responded:
[238] June 2, 2015 at 9:04pm
Minnesota once again… And people think Calipornia is nuts.
In reply to oldguy49's comment on the story Parents Livid Over 'Democratic' School's Shock Field Trip — but the Director Says It Was 'Beautiful'
[13] June 2, 2015 at 9:11pm
BlackCrow responded:
[33] June 2, 2015 at 9:39pm
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[27] June 2, 2015 at 9:47pm
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[27] June 2, 2015 at 9:50pm
Excomunicatedmarine responded:
[30] June 2, 2015 at 9:55pm
Since.I.Gave.Up.Hope.I.Feel.A.Lot.Better responded:
[29] June 2, 2015 at 10:00pm
MN NICE responded:
[13] June 2, 2015 at 10:08pm
64FNORD responded:
[9] June 3, 2015 at 9:57am
CutThePuppetStrings responded:
[6] June 2, 2015 at 3:20pm
Minnesota makes California look almost sane by comparison…
Skol Vikings!!!
[5] June 2, 2015 at 3:20pm
Minnesota makes California look almost sane by comparison…
Skol Vikings!!!
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Artist Zachary Thornton
"Yellow Dress" by Parkville artist Zachary Thornton is one of several of the narrative portrait artist's works on exhibit at C. Grimaldis Gallery through the end of March. Thornton often sets his subjects in local neighborhoods, including Stoneleigh and Roland Park. Staff photo by Brian Krista
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Samsung's 2012 flagship device, the Galaxy S III (S3) has made its way over to AT&T and is available in three colors: Red, White, and Blue. The AT&T Galaxy S III features a stunning 4.8-inch HD Super AMOLED display with a 1280 x 720 resolution and runs on Android 4.0 (ICS).
Unlike its European relative, the AT&T Galaxy S III runs with a Qualcomm Snapdragon S4 1.5GHz dual-core processor along with 2GB of RAM and up to 16GB of internal storage. An 8-megapixel camera compliments the rear of the device, as well as a 1.9MP front-facing camera.
Top AT&T Galaxy S3 Stories
Recent AT&T Galaxy S3 Forum Threads
- What is the easiest way to get pics into iPhoto
- Video is too big to send in a message
- Unfortunately, the process com.google.process.gapps has stopped.
- deleting pics & videos on Photos app Update
- Visual Voice mail
- IP Address Not Available?
- Need a little bit of help
- Phone calls people by itself
- Mobile Data Not Working and Phone is Rebooting (Works fine with WiFi)
- Any way to exclude a folder from the Gallery App?
- See All Threads


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Town Square
News Digest
Original post made on Mar 29, 2013
Read the full story here Web Link posted Friday, March 29, 2013, 12:00 AM
Comments (1)
a resident of Greenmeadow
on Mar 29, 2013 at 9:54 am
As a Greenmeadow resident, I resent having to take the extra time to try and figure out what garbage goes where. I pay a lot of money already for my garbage pick-up. Let the employees do the sorting, not the residents. Sorry, but I guess I am not a green resident of Greenmeadow. All of my trash will be going in one bag, as usual, and the recyclables will go, loosely, in the same can - green or blue, I have no idea.
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Lutticken's owner opens Main & Elm in Redwood City
By Elena Kadvany | 7 comments | 2,393 views
OK I Changed The Title
By Laura Stec | 22 comments | 1,649 views
Family Outing - Inside Out
By Cheryl Bac | 0 comments | 837 views
Relay for Life, Menlo Park, August 8
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I simply love this restaurant. Almost every here, my family and I come here for Christmas and Easter and we always find great food and excellent services.
The sweets are the best I've ever tasted, especially their ice cream with caramelized walnuts.
5 of us dropped in for lunch whilst passing and had an excellent meal in a friendly atmosphere. I can understand how Pavarotti became so large now. Good wine list with reasonable prices. I had the risotto as the main meal but there was a good selection chosen by the group and everyone loved it
Typical authentic Italian restaurant, and in the best way. All staff are courteous and pleasant, and food is quite exceptional. A must to visit if around Modena.
We went for dinner with family. Someone else made our reservations and warned them that we were American and spoke very little Italian. They went out of their way to make us comfortable and we had an incredible meal. And once the waiter saw how much my 7 year old son liked the gnocco fritto, he kept bringing more. It...
Visited on night 2 of our 3 night stay. Was our anniversary and want rather dressed up. Only us to start with and then another 2 tables filled
Ordered a cheese starter was greasy and then 2 types if pasta to taste. These were warm not hot
The waiter hovered around a lot and it was uncomfortable at times
In times of mass produce of food Europa 92 is a hidden gem .. Perfect deco and absolute delicious food , please if are snobbish and go to restaurants to be the center of attention don't go , this special restaurant is for food, music and wine lover..
While visiting family in Modena, they took us to Europa 92 to celebrate a birthday. The location and decor really added to our Italian experience. We loved all the pictures of Pavarotti and the charming decor. Our waiter was so friendly! The service and food were excellent! I would love to eat here again!
The food was really good. We were in a party of 19 people and all the food came out hot and very tasty. They gave us a selection of starters and pickles. Black rice was different. Lots of people in an unusual setting. Seemed to be in an awning.
When you are driving with four friends at 2:00pm on Sunday afternoon desperate for something to eat and use Tripadvisor, it's difficult to read the map. We ended retracing the last 10 minutes of driving end were heading further from Modena thinking are they open. Thankfully Europa 92 was open and accepted us in.
Once seating we were poured a...
I was in Europa 92 during my business travel in Italy . Italian friends suggested me this restaurant .great atmosphere inside and really wonderful menu ... the owner introduce me the best fresh dishes with great passion ... i liked this feeling .. now i know better what is Modenese meals and balsamico di Modena. Supertasting ! full of stories...
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You can now invite anyone to meet at your favorite Starbucks.
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47 year old man | Toluca Lake, CA, USA | Seeking women 23-35 within 50 miles of North Hollywood, CA
There are never problems, only solutions.
I'm a hard working man. I take joy and pride in my family. I believe trust, respect and truthfulness is the key to a good relationship. I'm very easy going and those who I considered my friends can count on me every time. I want someone I can be best friends with , someone I can talk to and have a laugh. I want to be able to ask how your day was and make it better if I can. I like light hearted people to whom smiling comes easily.
Camping , Coffee and conversation , Business networking , Cooking , Dining out , Movies/Videos , Museums and art , Music and concerts , Exploring new areas , Nightclubs/Dancing , Performing arts , Playing cards , Playing sports , Shopping/Antiques , Travel/Sightseeing , Video games , Watching sports , Wine tasting
Auto racing / Motorcross, Basketball, Billiards / Pool, Bowling, Cycling, Football, Golf, Hockey, Martial arts, Other types of exercise, Running, Soccer, Swimming, Walking / Hiking, Weights / Machines, Yoga
I exercise 3-4 times per week.
I have Dogs, Fish
I like Exotic Pets
Brazil, New York, Las Vegas, Malibu, Venice, Hollywood.
Like to go driving to new spots. Take a day or two off to have fun out of L.A. Find the best Sushi Bar ever. Find a Timeless spot and call it my own.
I like to go hiking with my dogs, movies, getting out and enjoying the day, whether at some random flee market or just having brunch with really good friends.
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What he is looking for
|5'9" (175cms)
|4'10" (147cms) to 5'10" (177cms)
|Body type:
|About average
|No preference
|No preference
|No preference
|No Way
|No preference
|Social Drinker
|No preference
|Self-Employed / Entrepreneur
|No preference
I do post production.
|No preference
|Never Married
|No preference
|Have kids:
|No preference
|Wants kids:
|Not sure
|No preference
|Latino / Hispanic, White / Caucasian
|No preference
Im from Brazil and i moved here when i young.
|Christian / Catholic
|No preference
|English, Italian, Portuguese, Spanish
|No preference
|Some college
|No preference


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List all words ending with ultrarare
Only the word ultrarare was found, nothing longer.
Some random words: mo ogam deoxidation li up blab roach
This is not a dictionary, it's a word game wordfinder.
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Rudy Giuliani, once a tough-on-crime mayor of New York City, has a new mission. In a cover story for Hamptons magazine, the 70-year-old widely known for working 9/11 into every sentence he uttered on the campaign trail declared that golf is too complicated, and too difficult.
The solution, Giuliani said, is to "make it simpler and easier."
"Let's shorten the course and make playing 12 holes instead of 18 holes the norm," Giuliani suggested. "I've played tennis for 20 years, and I've never played five sets like the pros do. I'll play three sets with my pals. So why play 18 holes? That's what pros do to win tournaments."
Like stifling humidity and 90-degree weather, it seems the biggest Hamptons Problem has arrived after Labor Day this year. Rudy Giuliani is a man who once dared to run for president of the United States of America, and he thinks a round of golf is too confusing? This guy took on the mob as a federal attorney, switched his political party twice, and was ceremoniously knighted by the Queen of England.
Meanwhile, according to the questionable Web site obamagolfcounter.com, our current commander in chief has hit the links 192 times. Right or wrong, Obama has navigated the game's complexities time and time again while concurrently deciding how to take on ISIS, quell the unrest in Ferguson, and stare down Putin.
Then again, maybe America's Mayor is right. Could the president lift his approval ratings above 50 percent by calling it a day six holes early?
For more high-profile interviews, stunning photography, and thought-provoking features, subscribe now to Vanity Fair magazine.


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Select Local Merchants
Safari Sun TanningRead MoreLooking for an equal spray tan? Head to Safari Sun Tanning in Decatur for an even and sun-kissed tan. With services so full, you can spend a day relaxing indoors at Safari Sun Tanning. It'll even make it look like you went outside with its tanning service. This salon is the go-to place in the area for professional alternative services like acne treatment. Freshen up your look and find all the best tanning deals at Safari Sun Tanning — you won't be disappointed!2899 N Decatur RdDecatur, GA US
Solar DimensionsRead MoreWith 20 locations conveniently sprinkled throughout the Atlanta area, Solar Dimensions helps clients get beach-ready regardless of their proximity to the shore. Each location features a fleet of UV tanning beds of varying brightness, all waiting to sun-kiss skin in 10?20 minutes and comfort clients with a built-in cooling apparatus or complimentary snuggles from a nearby penguin. Custom airbrush-tanning treatments or automated VersaSpa and MyMyst sunless-tanning sessions forgo light exposure and instead tone complexions with tinted mists that materialize in four to eight hours and last for several days. Both tanning solutions quench skin with aloe vera before tinting it a natural shade ranging from light almond to lifeguard. The trained staff can also hone body shape with infrared body wraps and VacuStep treatments, and brighten smiles with teeth whitening, helping to boost confidence before bodies hit the pool.950 W Peachtree St.Atlanta, GA US
Ansley BeachRead MoreMissing the beach? Atlanta's Ansley Beach offers customized tanning services so you won't have to leave that beach glow behind. When you're running low on contact lenses, Ansley Beach has many different kinds to offer. While you're there, you should also check out the wide selection of glasses. Ansley Beach offers tanning services for every skin type, such as tanning beds, spray tan, and airbrush tan. Go beyond mainstream medical care and try an alternative service from this salon. Appointments aren't needed to receive a beauty treatment from this salon. Payment is only a swipe away at Ansley Beach with any major credit card. At Ansley Beach, drivers can settle for safe parking in the lot next door. Tanning is always available to you at Ansley Beach so call and book your appointment today.1544 Piedmont Ave NEAtlanta, GA US
Hollywood Tans AtlantaRead MoreAt (STAND) UV & Spray Tanning, tanners choose from a variety of traditional and UV-free bronzing methods. With an effect similar to tying the sun around a tetherball pole, standup UV booths feature 360-degree tanning, deepening skin tone during expedient 8- to 11-minute sessions. Patrons opting for sunless tanning can choose between automated Mystic Tan sessions with a choice of four shades of tan or custom Norvell airbrush treatments applied by trained technicians. Additionally, red-light-therapy sessions unclench faces from the grip of Father Time by reducing the appearance of fine lines and large pores, as well as boosting elasticity.3015 N Druid Hills Rd NeAtlanta, GA US
Faux Real PaintingRead MoreFaux Real Painting in Atlanta offers multiple tanning services for any skin type. Faux Real Painting offers tanning services for every skin type, such as tanning beds, spray tan, and airbrush tan. This salon offers a wide range of alternative services that will meet or even exceed your needs, such as acne treatment. If you're planning a trip to somewhere warm, head on over to Faux Real Painting for a base glow.370 Northside Dr NWAtlanta, GA US
Pure Sol Body Bronzing ExpertsRead MoreCertified technicians bring our UV FREE/SUNLESS Airbrush Body Bronzing System to you. Your location, Your Schedule. We offer the most Beautiful, Flawless,Natural Airbrush tan in Atlanta. The Quality of the products and the Expertise of the Custom and Personalized airbrush application are what set us apart from all others.4780 Ashford Dunwoody Road NortheastAtlanta, GA US


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Tech at Night: The government wants your data protected, except when it doesn't.
Sometimes the government cheers the idea that your data is being protected from the bad guys. Other times, the government grumbles and complains. It turns out they're fine with your data being at risk, as long as it means government can get to it whenever it wants. Funny, that.


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Spiral Pipe Mfg Co Inc
0 reviews
11419 Yellow Pine St NW, Minneapolis, MN 55448
(000) 000-0000
Owner Message
Spiral Pipe Mfg Co., Inc. offers a complete line of metal air ducts and supplies for commercial and industrial applications. We manufacture lockseam, high pressure spiral pipe, and fittings at our plant in Minneapolis, Minnesota and ship products throughout the country.
Spiral is made from light-gauge metal and designed for maximum strength, making it ideal for above and below ground installation. It is well suited for all types of ventilation including grain handling, dust removal, carbon monoxide exhaust and dual wall pipe for thermal and sound insulation.
We offer a complete line of metal air ducts for commercial and industrial spiral pipe and fittings, custom duct and duct fittings for HVAC and dust collecting systems, along with a comprehensive line of chemical and underground duct.
Our products and services include:
-High, low and medium pressure pipe and fittings
-Dust collection and pneumatic conveying distribution systems
-Stainless steel and PVS coated spiral pipe
-Elbows and connectors
-Custom fabrication
Spiral pipe is attractive and suitable for warehouse and other areas where it remains exposed. The surface can be painted to blend or stand out from the surrounding environment. In addition, spiral duct piping is economical and easy to install.
Our products can be used in a variety of settings. Call to speak with a customer service representative today to learn how spiral pipe can be incorporated into your business.
Call us today for more information!
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Sheet Metal Work
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1996 Chrysler Town & Country Q&A
1996 Chrysler Town & Country Question: Where is the speed sensor located?
My van won't always shift gears. It's an automatic, 3.8L, V6. - Mike Meyer
2 Answers
Answer 1
it may or maynot be your problem. there is an spped sensor on the front side of the trans. get it checked before you throw expensive parts at it. Roy - mastertech6371
Answer 2
NO, Just raise the front of the vehicle, place a pan under the front on the transmission oil pan and replace the sensor. - Johnny Mopar
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odometer is working but not the speedometer is not .i know it's a sensor but which one?it has an input and a output but will not show up on a computer check as to which one it is.thanks
need to know where they are so i can change them have already got them
I'm getting code P0700 and code p0720. I was told its the output speed sensor and I need to know where this is located on my van
Can someone tell me in detail where its located. I am an amateur and have only done a few things. I cant afford the 400.00 the auto repair shop is giving me. Any pics would be great. I get an error...
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Addams Family: Left in Lurch presents four issues from the animated version of the monster family TV series. Included here are: "Left in the Lurch," "The Mardi Gras Story," "The Voodoo Story" and "Aloha Hoolamagoola." ~ Rovi
Ted Cassidy, Jackie Coogan, Jodie Foster, View All »
Run Time 90 minutes


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Nigeria: Multichoice Engages 100 people On Entrepreneurial Scheme
In Aba, Multichoice Nigeria says it has invested huge sums of money into its digital transmission network to deliver quality digital television service in Nigeria.
General manager of GOtv (pay DTT), a subsidiary of Multichoice, Elizabeth Amkpa, who disclosed this during the launch of its digital terrestrial television platform in Aba, the commercial centre of Abia state, said it is in line with the target of digital migration by 2015.
17 to 19 July 2013Mediatech Africa 2013Venue: Coca-Cola Dome in Northgate, Johannesburg, South AfricaThe biennial advanced technology trade show has positioned itself as the largest and most prestigious event of its kind in Africa for the media and entertainment industries. For more information click here.
'Digital Services Africa' invites you to secure your pass at this year's show, taking place on 25 - 26 June in Johannesburg.
About 180+ operators and enterprises are already registered.
Nigeria: 'Govt, Not Nollywood, Should Cater for Film Veterans' says Elliot
Nollywood actor-cum-director, Desmod Elliot has said taking care of veteran of Nollywood actors who fall sick is beyond the industry as the government ought to cater for movie stars.
The Glo ambassador expressed dissatisfaction with the rate Nollywood veterans die because of poor medical attention and the inability to foot their hospital bills.
He said "it is not the industry, this is beyond the industry, it is what the government should do something about."
6-23 June 2013Encounters - South African International Documentary Film Festival Capte Town, South AfricaEncounters is Africa's premier documentary event, celebrating its 15th edition in 2013.For more information click here:
Uganda Communications Commission Executive Director, Godfrey Mutabaazi, has issued a statement that all free to air television stations will air 70% local content. This is indeed an admirable stance and he should be commended for his good intentions. However, I think he also needs to look at the current realities on the ground and perhaps understand the industry better.
The UCC has supposedly looked at other countries for guidance and set the standard based on those countries. These countries are SA, Australia and the UK, among others.
Call for entries: Al Jazeera Pitching Forum at Encounters
On Saturday 15 June 2013 in Cape Town, two commissioning editors from Al Jazeera will listen to pitches from 16 African filmmakers. Each person has a maximum of five minutes to pitch their film, with an additional 10 minutes allocated for feedback and questions from the panel. Visual aids will be provided for those that have DVDs or presentations during the pitch.
SA's sci-fi siren set to seduce Hollywood
A week after her latest novel, The Shining Girls, hit the Sunday Times Bestseller list in the UK, Lauren Beukes began negotiating film rights to the book.
This is on top of South African movie producer Helena Spring acquiring rights to Zoo City - Beukes' previous novel, which won the Arthur C Clarke and Kitschies Red Tentacle literary awards.
Health journalism contest offers US study tour - Apply by May 20, 2013.
Professional and freelance journalists working in Sub-Saharan Africa, the Gulf states and Pakistan can enter a contest recognizing the best media coverage of vaccines and immunization.
Winners from each region will receive cash prizes and a two-week study tour to the United States to meet health experts.


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2013 Fed 100
Name: Douglas WiltsieTitle: Program Executive Officer for Enterprise Information SystemsAgency: Department of the ArmyProgram wrangler. The scope of programs under Wiltsie's purview is enough to make most mortals shudder. He leads an organization with more than 40 acquisition programs that cross all domains. He works with an unprecedented 15 functional stakeholders to build a cohesive team and foster information sharing. The results speak for themselves. Take, for instance, the five major enterprise resource planning systems, two of which achieved significant acquisition milestones last year. The $8 billion investment improved financial management and visibility of global assets for the service, allowing Army leaders to make better decisions.
Click here for the full list
About the Author
Connect with the FCW staff on Twitter @FCWnow.
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Meet 100 women and men who are doing great things in federal IT.
Click here for the full list
Three-time Federal 100 winner is leaving government in July.
Members of Congress heaped hard questions on federal IT leaders at a June 16 hearing and suggested strongly that somebody needs to be fired.
Senate bill carves out exemption for supercomputing and other lab IT; an earlier version of the measure would have exempted the entire Department of Energy.
OPM is partnering with CSID to try to manage the fallout from a massive breach of some 4 million federal personnel records.
Steve Kelman applauds the OMB's move to make career employees "goal leaders" for performance management initiatives -- but wishes it had come far earlier in the administration.
In an exclusive interview, Jimaye Sones, who was Defense Information Systems Agency comptroller from 2005 to 2013, says he was reassigned after revealing questionable accounting practices at the agency.
Through computer forensics training and internships, veterans are helping Immigration and Customs Enforcement tackle a backlog in child exploitation cases.
After two months on the job studying DOD's cybersecurity and IT needs, Secretary Ashton Carter is set to unveil a new Pentagon cyber strategy in Silicon Valley.
The failure of massive federal IT projects can usually be traced to poor management. The solution is as multilayered as the projects themselves.
8609 Westwood Center Drive, Suite 500Vienna, VA 22182-2215 703-876-5100
© 1996-2013 1105 Media, Inc. All Rights Reserved. This copy is for your personal, non-commercial use only.To order presentation-ready copies for distribution to colleagues, clients or customers, visit: www.1105Reprints.com


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3 bed terraced house for sale
£795,000 Guide Price
- 16
- 0
Guide Price £795,000
Guest House for sale Esplanade,West Bay DT6CONTACT HOLLOWAY ESTATE AGENTS TO VIEW: West Bay (Broadchurch) SEVEN HOLIDAY APARTMENTS PLUS OWNERS SUITE. OUTSTANDING PANORAMIC SEA VIEWS Located opposite Jurassic beach promenade in quaint holiday/fishing village of West Bay. More details ›
£725,000 Guide Price
Guide Price £725,000
8 bedroom detached house for sale West Bay Road, Bridport, DorsetA superb home and income opportunity a successful and well established guest house, ideally situated between the market town of Bridport and the coastal resort of West Bay Freehold More details ›
£595,000 Guide Price
Guide Price £595,000
2 bedroom bungalow for sale Fourth Cliff Walk, West Bay, Bridport, Dorset, DT6 4HLA detached coastal bungalow set in grounds of approximately 0.31 acre providing sea views over Lyme Bay with potential for development, subject of course to any necessary consents. More details ›
Added on 19/05/2015 by Symonds & Sampson, Bridport. Call: 01308 318001 Local call rate
£465,000 Guide Price
Guide Price £465,000
2 bedroom apartment for sale Bridport, DorsetPenthouse apartment with two terraces and sea and harbour views. Excellent investment and/or holiday home/permanent residence. Open plan living area. Terrace and balcony with sea views. Kitchen/dining space. 2 double bedrooms. Family bathroom. Secure entrance and parking. Close to amenities. EPC ... More details ›
Reduced on 24/06/2015 by Stags, Bridport. Call: 01308 318008 Local call rate
£465,000 Offers in Excess of
Offers in Excess of £465,000
3 bedroom terraced house for sale West Bay**OPEN HOUSE - SATURDAY 27TH JUNE 2015 11AM - 1PM** STUNNING HARBOURSIDE TOWNHOUSE - Set along the harbour at West Bay, this 3 double bedroom property enjoys open plan living and balconies enjoying seaside views - NOT TO BE MISSED More details ›
£425,000 Guide Price
Guide Price £425,000
2 bedroom apartment for sale Bridport, DorsetAn idyllically positioned waterside apartment in the popular harbour village of West Bay. 2 double bedrooms (one en suite). Open plan living area and kitchen, family bathroom, outside terrace/balcony and further balcony, beautiful sea views and parking. EPC Band B. More details ›
Reduced on 02/07/2015 by Stags, Bridport. Call: 01308 318008 Local call rate
4 bedroom bungalow for sale Hill Close, West Bay, Dorset DT6Superb four bedroom detached bungalow located in West Bay, Bridport. More details ›
Reduced on 03/03/2015 by My Online Estate Agent, National. Call: 0800 009 8030 Call rates may vary
4 bedroom detached house for sale FOURTH CLIFF WALK, West Bay,VIEWING RECOMMENDED A Detached Individual House with SEA AND RURAL VIEWS situated above the coastal village of West Bay. More details ›
4 bedroom detached house for sale West BayViewing recommended to appreciate this Detached COASTAL BUNGALOW situated on an elevated site in cul-de-sac with extensive views. Double Garage. NO FORWARD CHAIN. WEST BAY (BROADCHURCH) More details ›
£395,000 Guide Price
Guide Price £395,000
2 bedroom flat for sale West BayCONTEMPORARY LIVING IN A HARBOURSIDE LOCATION - This ground floor apartment is presented to a high specification and enjoys secure parking and balcony - VIEW TODAY More details ›
Thank you.
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Up To 50% Off Top Beauty & Fragrance In Our Summer Sale - Ends Monday. Save now
Great product, keeps lips soft without the nasties. Happy for my daughter to use them too.
Works well
Évaluation du service : Wide range with products, will buy again from this site.
Produit : An excellent product with natural organic ingredients! Will repurchase.
Balm is really good and soften my lips within just seconds.
for my nine year old and her friends
Keep lips lovely and soft
fun lip balms in lovely scents.
Natural ingredients and very moisturising.
Although the product is good , it starts breaking up in the tube after few application. Manufacturers, please note.
Great, very cute
Good product been using these for years
All round excellent!
Quick delivery. Slightly confused about first order promotion as I thought you received a discount but didn't get one (maybe I misread on homepage).
Quick service and great selection of products.
Great service as always.
Good products, fast delivery, will use again.
My husband is pleased.
always very happy with service
Have just started using the vanilla lip balm from this pack.Am very happy. It is very moisturising without being sticky.
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text: Public Service Announcements. By Monika Guendner • Jul 19, 2013 ShareTwitter Facebook Google+ Email State Liquor Stores are closed for a holiday. KPCW airs this as a public service announcement. Tags: only in Park CityPSAsLiquor Stores Related Content It's a Cat's Life. 1 year ago Moose Reports. 1 year ago Ask For One, Get Two. 1 year ago View the discussion thread.


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- An ISBN is the 10 or 13 digit number, usually printed on the back of a book with a bar code, that serves as a unique identifier for the book.
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AUTHORS: Tursi/O'Connor - ©2012
The workbook portion of the SAM includes the Pratica del vocabolario e della struttura section, to be completed after studying the corresponding text material, which provides reinforcement practice of the lesson vocabulary and grammar topics individually. The Pratica della comunicazione section, completed at the end of each text lesson, contains open-ended and communicative exercises to integrate the lesson material. The lab manual portion is designed for use with the SAM Audio Program. Written and oral exercises reinforce lesson structures and vocabulary and provide additional pronunciation practice. Also included are the dialogues from the text and numerous activities based on conversations, recorded messages and announcements, and descriptions to help you develop listening comprehension skills.


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Theola J Boeckman
Saint Marys, KS
Basic information on Theola J Boeckman, Saint Marys KS. Find Theola J Boeckman's age, gender, possible aliases, associates and professional status.
Also Known As
Theola J Boeckman
Theola J Boeckman is in his 60's
Theola Has Lived In
Saint Marys, KS
Alma, KS
Goff, KS
Theola's Relatives
Theola J Boeckman
Zodiac SignGemini
Additional Infomation on Theola J Boeckman
Theola J Boeckman's Contact Information
Known Cities Lived In
Find out where Theola J Boeckman has lived as well as Theola J Boeckman's phone numbers and email addresses.
Theola J Boeckman Has Lived in 1 State
Kansas Address for Theola J Boeckman
205 S 4xx Sx
Saint Marys KS
Saint Marys KS
Has Lived InGet Full Address Report
Saint Marys, KS (2 Addresses)
Alma, KS
Phone Numbers Associated with Theola J Boeckman
Get Full Phone Report
(785) ***-**** - Baldwin City, KS
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