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Are you Claus-Wilhelm von der Lieth?Claim your profile
Publications (63)176.68 Total impact
- SourceAvailable from: Roland Stenutz[Show abstract] [Hide abstract]
ABSTRACT: The EUROCarbDB project is a design study for a technical framework, which provides sophisticated, freely accessible, open-source informatics tools and databases to support glycobiology and glycomic research. EUROCarbDB is a relational database containing glycan structures, their biological context and, when available, primary and interpreted analytical data from high-performance liquid chromatography, mass spectrometry and nuclear magnetic resonance experiments. Database content can be accessed via a web-based user interface. The database is complemented by a suite of glycoinformatics tools, specifically designed to assist the elucidation and submission of glycan structure and experimental data when used in conjunction with contemporary carbohydrate research workflows. All software tools and source code are licensed under the terms of the Lesser General Public License, and publicly contributed structures and data are freely accessible. The public test version of the web interface to the EUROCarbDB can be found at http://www.ebi.ac.uk/eurocarb.Glycobiology 11/2010; 21(4):493-502. DOI:10.1093/glycob/cwq188 · 3.75 Impact Factor
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ABSTRACT: GlycomeDB integrates the structural and taxonomic data of all major public carbohydrate databases, as well as carbohydrates contained in the Protein Data Bank, which renders the database currently the most comprehensive and unified resource for carbohydrate structures worldwide. GlycomeDB retains the links to the original databases and is updated at weekly intervals with the newest structures available from the source databases. The complete database can be downloaded freely or accessed through a Web-interface (www.glycome-db.org) that provides flexible and powerful search functionalities.Nucleic Acids Research 11/2010; 39(Database issue):D373-6. DOI:10.1093/nar/gkq1014 · 9.11 Impact Factor
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ABSTRACT: The GlycoViewer (http://www.systemsbiology.org.au/glycoviewer) is a web-based tool that can visualize, summarize and compare sets of glycan structures. Its input is a group of glycan structures; these can be entered as a list in IUPAC format or via a sugar structure builder. Its output is a detailed graphic, which summarizes all salient features of the glycans according to the shapes of the core structures, the nature and length of any chains, and the types of terminal epitopes. The tool can summarize up to hundreds of structures in a single figure. This allows unique, high-level views to be generated of glycans from one protein, from a cell, a tissue or a whole organism. Use of the tool is illustrated in the analysis of normal and disease-associated glycans from the human glycoproteome.Nucleic Acids Research 07/2010; 38(Web Server issue):W667-70. DOI:10.1093/nar/gkq446 · 9.11 Impact Factor
Article: Corrigendum to ''A lectin from the Chinese bird-hunting spider binds sialic acids" [Carbohydr. Res. 344 (2009) 1515]Carbohydrate Research 04/2010; 345(6):860. DOI:10.1016/j.carres.2010.01.003 · 1.97 Impact Factor
Article: Why Structurally Different Cyclic Peptides Can Be Glycomimetics of the HNK-1 Carbohydrate Antigen [].Journal of the American Chemical Society 02/2010; 132(10). DOI:10.1021/ja100344v · 11.44 Impact Factor
Article: WITHDRAWN: Corrigendum to "A lectin from the Chinese bird-hunting spider binds sialic acids"[Show abstract] [Hide abstract]
ABSTRACT: The Publisher regrets that this article is an accidental duplication of an article that has already been published, doi: 10.1016/j.carres.2010.01.003. The duplicate article has therefore been withdrawn.Carbohydrate research 02/2010; DOI:10.1016/j.carres.2009.12.027 · 1.97 Impact Factor
Article: Why Structurally Different Cyclic Peptides Can Be Glycomimetics of the HNK-1 Carbohydrate Antigen[Show abstract] [Hide abstract]
ABSTRACT: The cyclic peptides c-(LSETTl) and c-(RTLPFS) are of potential clinical interest--they stimulate neurite outgrowth in a way that is similar to the effects of the HNK-1 (human natural killer cell-1) antigenic carbohydrate chains, which are terminated by 3'-sulfated glucuronic acid attached to an N-acetyllactosamine unit. To investigate the structure-activity relationships of the ability of the cyclic peptides to mimic HNK-1 carbohydrates, conformational analysis and examination of hydrophobic and hydrophilic patterns were performed and compared with the characteristics of a synthetic HNK-1 trisaccharide derivative. Data obtained demonstrate that both the trisaccharide and the glycomimetic peptide c-(LSETTl) exhibit a similar relationship between their hydrophobic moieties and their negatively charged sites. However, the second cyclic glycomimetic peptide investigated here, c-(RTLPFS), has a positively charged group as a potential contact point due to its Arg residue. Therefore, we studied the amino acid composition of all known receptor structures in the Protein Data Bank that are in contact with uronic acid and/or sulfated glycans. Interactions of the HNK-1 trisaccharide, c-(LSETTl), and c-(RTLPFS) with a laminin fragment involved in HNK-1 carbohydrate binding (i.e., the 21mer peptide: KGVSSRSYVGCIKNLEISRST) were also analyzed. Because the structure of the HNK-1-binding laminin domain is not available in the Protein Data Bank, we used the HNK-1-binding 21mer peptide fragment of laminin for the construction of a model receptor that enabled us to compare the molecular interplay of the HNK-1 trisaccharide and the two cyclopeptides c-(LSETTl) and c-(RTLPFS) with a reliable receptor structure in considerable detail.Journal of the American Chemical Society 01/2010; 132(1):96-105. DOI:10.1021/ja904334s · 11.44 Impact Factor
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ABSTRACT: Yes YesBioinformatics for Glycobiology and Glycomics: An Introduction, 11/2009: pages 21 - 47; , ISBN: 9780470029619
- Bioinformatics for Glycobiology and Glycomics: An Introduction, 11/2009: pages 433 - 445; , ISBN: 9780470029619
Chapter: Glycosylation of Proteins[Show abstract] [Hide abstract]
ABSTRACT: Glycosylation is the most common and most complex co- and posttranslational modification of proteins. Glycan chains can alter the physico-chemical properties of glycoproteins, and N-glycans also play an important role in the protein folding process. Glycosylated proteins on cell surfaces are implicated in various cell-cell and cell-matrix interactions. This chapter gives an overview of the various glycosylation types, and the functions and biosynthesis of the carbohydrate chains. A statistical analysis of the amino acids in the neighborhood of occupied glycosylation sites reveals different patterns of frequently found amino acids around N-glycosylation and O-glycosylation sites.Bioinformatics for Glycobiology and Glycomics: An Introduction, 11/2009: pages 143 - 162; , ISBN: 9780470029619
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ABSTRACT: Various databases that provide information on glycoenzymes are available on the Internet. In addition to the common protein databases there are several resources that specialize in glycoenzymes or contain an individual subsection for this kind of enzyme. This chapter provides an overview of existing databases aside from CAZy, such as the GlycoGene DataBase, KEGG, the CFG databases, ExPASy ENZYME, and BRENDA, and lists the kind of data that can be found in the individual resources. The majority of the specialized databases contain information on glycosyltransferases involved in glycan biosynthesis, while data on carbohydrate degrading enzymes is less often found.Bioinformatics for Glycobiology and Glycomics: An Introduction, 11/2009: pages 119 - 123; , ISBN: 9780470029619
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ABSTRACT: Despite ongoing harmonization efforts, the major carbohydrate sequence databases following the first initiative in this field, CarbBank, are still isolated islands, with mechanisms for automatic structure exchange and comparison largely missing. This unfavorable situation has been overcome with a systematic data integration effort, resulting in the GlycomeDB, a meta-database for public carbohydrate sequences. It contains at present 35,056 unique structures in GlycoCT encoding, referencing more than 100,000 external records from 1845 different taxonomic sources. We have created a user-friendly, web-based graphical interface which allows taxonomic and structural data to be entered and searched for. The structural search possibilities include substructure search, similarity search, and maximum common substructure. A novel search refinement mechanism allows the assembly of complex queries. With GlycomeDB (www.glycome-db.org), it is now possible to use a single portal to access all digitally encoded, public structural data in glycomics and to perform complex queries with the help of a web-based user interface.Glycobiology 09/2009; 19(12):1563-7. DOI:10.1093/glycob/cwp137 · 3.75 Impact Factor
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ABSTRACT: The affinity to sialic acid-containing oligosaccharides of the small-animal lectin SHL-I isolated from the venom of the Chinese bird-hunting spider Selenocosmia huwena is here described for the first time. By a strategic combination of NMR techniques, molecular modeling, and data mining tools it was possible to identify the crucial amino acid residues that are responsible for SHL-I's ability to bind sialic acid residues in a specific way. Furthermore, we are able to discuss the role of the functional groups of sialic acid when bound to SHL-I. Also the impact of Pro31 in its cis- or trans-form on SHL-I's ligand affinity is of special interest, since it answers the question if Trp32 is a crucial amino acid for stabilizing complexes between SHL-I and sialic acid. SHL-I can be considered as a proper model system that provides further insights into the binding mechanisms of small-animal lectins to sialic acid on a sub-molecular level.Carbohydrate research 07/2009; 344(12):1515-25. DOI:10.1016/j.carres.2009.06.002 · 1.97 Impact Factor
- 01/2009; John Wiley & Sons., ISBN: 978-0-470-01667-1
Article: Conformational analysis of the neutral exopolysaccharide produced by Lactobacillus delbrueckii ssp. bulgaricus LBB.B26[Show abstract] [Hide abstract]
ABSTRACT: The conformational properties of the neutral exopolysaccharide produced by Lactobacillus delbrueckii ssp. bulgaricus LBB.B26 have been studied by NMR measurements and molecular modelling. The exopolysaccharide, with an average molecular mass of 1.3 x 10(6) Da, was previously determined to consist of pentasaccharide repeating units with the following structure: -->3)-beta-D-Galp-(1-->4)-beta-D-Glcp-(1-->3)-beta-d-Galf-(1-->3)-[alpha-D-Glcp-(1-->6)-]alpha-D-Galp-(1-->. Adiabatic maps were generated for each of the disaccharide fragments in the repeating unit. In addition, free energy maps calculated from MD simulations were obtained for each of the glycosidic linkages in an extended repeating unit and the influence of the Galf residue on the conformational properties of the repeating unit was investigated. The values of the global energy minima provided by the free energy maps were used to build a polymer chain. The polysaccharide was shown to have a random coil structure, without stable extended helical motifs.Organic & Biomolecular Chemistry 01/2009; 7(2):280-7. DOI:10.1039/b810468a · 3.49 Impact Factor
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ABSTRACT: Although carbohydrates are the third major class of biological macromolecules, after proteins and DNA, there is neither a comprehensive database for carbohydrate structures nor an established universal structure encoding scheme for computational purposes. Funding for further development of the Complex Carbohydrate Structure Database (CCSD or CarbBank) ceased in 1997, and since then several initiatives have developed independent databases with partially overlapping foci. For each database, different encoding schemes for residues and sequence topology were designed. Therefore, it is virtually impossible to obtain an overview of all deposited structures or to compare the contents of the various databases. We have implemented procedures which download the structures contained in the seven major databases, e.g. GLYCOSCIENCES.de, the Consortium for Functional Glycomics (CFG), the Kyoto Encyclopedia of Genes and Genomes (KEGG) and the Bacterial Carbohydrate Structure Database (BCSDB). We have created a new database called GlycomeDB, containing all structures, their taxonomic annotations and references (IDs) for the original databases. More than 100000 datasets were imported, resulting in more than 33000 unique sequences now encoded in GlycomeDB using the universal format GlycoCT. Inconsistencies were found in all public databases, which were discussed and corrected in multiple feedback rounds with the responsible curators. GlycomeDB is a new, publicly available database for carbohydrate sequences with a unified, all-encompassing structure encoding format and NCBI taxonomic referencing. The database is updated weekly and can be downloaded free of charge. The JAVA application GlycoUpdateDB is also available for establishing and updating a local installation of GlycomeDB. With the advent of GlycomeDB, the distributed islands of knowledge in glycomics are now bridged to form a single resource.BMC Bioinformatics 10/2008; 9(1):384. DOI:10.1186/1471-2105-9-384 · 2.67 Impact Factor
Article: Statistical analysis of the Bacterial Carbohydrate Structure Data Base (BCSDB): Characteristics and diversity of bacterial carbohydrates in comparison with mammalian glycans[Show abstract] [Hide abstract]
ABSTRACT: There are considerable differences between bacterial and mammalian glycans. In contrast to most eukaryotic carbohydrates, bacterial glycans are often composed of repeating units with diverse functions ranging from structural reinforcement to adhesion, colonization and camouflage. Since bacterial glycans are typically displayed at the cell surface, they can interact with the environment and, therefore, have significant biomedical importance. The sequence characteristics of glycans (monosaccharide composition, modifications, and linkage patterns) for the higher bacterial taxonomic classes have been examined and compared with the data for mammals, with both similarities and unique features becoming evident. Compared to mammalian glycans, the bacterial glycans deposited in the current databases have a more than ten-fold greater diversity at the monosaccharide level, and the disaccharide pattern space is approximately nine times larger. Specific bacterial subclasses exhibit characteristic glycans which can be distinguished on the basis of distinctive structural features or sequence properties. For the first time a systematic database analysis of the bacterial glycome has been performed. This study summarizes the current knowledge of bacterial glycan architecture and diversity and reveals putative targets for the rational design and development of therapeutic intervention strategies by comparing bacterial and mammalian glycans.BMC Structural Biology 09/2008; 8:35. DOI:10.1186/1472-6807-8-35 · 2.22 Impact Factor
Article: Conformation of the c-Fos/c-Jun complex in vivo: a combined FRET, FCCS, and MD-modeling study.[Show abstract] [Hide abstract]
ABSTRACT: The activator protein-1 transcription factor is a heterodimer containing one of each of the Fos and Jun subfamilies of basic-region leucine-zipper proteins. We have previously shown by fluorescence cross-correlation spectroscopy (FCCS) that the fluorescent fusion proteins Fos-EGFP and Jun-mRFP1, cotransfected in HeLa cells, formed stable complexes in situ. Here we studied the relative position of the C-terminal domains via fluorescence resonance energy transfer (FRET) measured by flow cytometry and confocal microscopy. To get a more detailed insight into the conformation of the C-terminal domains of the complex we constructed C-terminal labeled full-length and truncated forms of Fos. We developed a novel iterative evaluation method to determine accurate FRET efficiencies regardless of relative protein expression levels, using a spectral- or intensity-based approach. The full-length C-terminal-labeled Jun and Fos proteins displayed a FRET-measured average distance of 8 +/- 1 nm. Deletion of the last 164 amino acids at the C-terminus of Fos resulted in a distance of 6.1 +/- 1 nm between the labels. FCCS shows that Jun-mRFP1 and the truncated Fos-EGFP also interact stably in the nucleus, although they bind to nuclear components with lower affinity. Thus, the C-terminal end of Fos may play a role in the stabilization of the interaction between activator protein-1 and DNA. Molecular dynamics simulations predict a dye-to-dye distance of 6.7 +/- 0.1 nm for the dimer between Jun-mRFP1 and the truncated Fos-EGFP, in good agreement with our FRET data. A wide variety of models could be developed for the full-length dimer, with possible dye-to-dye distances varying largely between 6 and 20 nm. However, from our FRET results we can conclude that more than half of the occurring dye-to-dye distances are between 6 and 10 nm.Biophysical Journal 05/2008; 94(7):2859-68. DOI:10.1529/biophysj.107.120766 · 3.83 Impact Factor
Article: HIV-1 Capsid Assembly Inhibitor (CAI) Peptide: Structural Preferences and Delivery into Human Embryonic Lung Cells and Lymphocytes[Show abstract] [Hide abstract]
ABSTRACT: The Human immunodeficiency virus 1 derived capsid assembly inhibitor peptide (HIV-1 CAI-peptide) is a promising lead candidate for anti-HIV drug development. Its drawback, however, is that it cannot permeate cells directly. Here we report the transport of the pharmacologically active CAI-peptide into human lymphocytes and Human Embryonic Lung cells (HEL) using the BioShuttle platform. Generally, the transfer of pharmacologically active substances across membranes, demonstrated by confocal laser scanning microscopy (CLSM), could lead to a loss of function by changing the molecule's structure. Molecular dynamics (MD) simulations and circular dichroism (CD) studies suggest that the CAI-peptide has an intrinsic capacity to form a helical structure, which seems to be critical for the pharmacological effect as revealed by intensive docking calculations and comparison with control peptides. This coupling of the CAI-peptide to a BioShuttle-molecule additionally improved its solubility. Under the conditions described, the HIV-1 CAI peptide was transported into living cells and could be localized in the vicinity of the mitochondria.International journal of medical sciences 02/2008; 5(5):230-9. · 1.55 Impact Factor
Article: Frontiers in glycomics: Bioinformatics and biomarkers in disease An NIH White Paper prepared from discussions by the focus groups at a workshop on the NIH campus, Bethesda MD (September 11–13, 2006)[Show abstract] [Hide abstract]
ABSTRACT: Key issues relating to glycomics research were discussed after the workshop entitled "Frontiers in Glycomics: Bioinformatics and Biomarkers in Disease" by two focus groups nominated by the organizers. The groups focused on two themes: (i) glycomics as the new frontier for the discovery of biomarkers of disease and (ii) requirements for the development of informatics for glycomics and glycobiology. The mandate of the focus groups was to build consensus on these issues and develop a summary of findings and recommendations for presentation to the NIH and the greater scientific community. A list of scientific priorities was developed, presented, and discussed at the workshops. Additional suggestions were solicited from workshop participants and collected using the workshop mailing list. The results are summarized in this White Paper, authored by the co-chairs of the focus groups.PROTEOMICS 01/2008; 8(1):8-20. DOI:10.1002/pmic.200700917 · 3.97 Impact Factor
Top Journals
Justus-Liebig-Universität GießenGieben, Hesse, Germany
Macquarie UniversitySydney, New South Wales, Australia
German Cancer Research Center
Heidelburg, Baden-Württemberg, Germany
- Division of Radiopharmaceutical Chemistry
University of Debrecen
Debreczyn, Hajdú-Bihar, Hungary
- Department of Biophysics and Cell Biology
Ludwig-Maximilian-University of Munich
München, Bavaria, Germany
- Faculty of Veterinary Medicine
Universität HildesheimHildesheim, Lower Saxony, Germany


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Welcome to the University of Sunderland
We are an innovative, forward-thinking university with high standards of teaching, research and support. We have strong links with industry and business, and work closely with some of the world's leading companies.
Our two Sunderland campuses and our London Campus are perfectly placed to ensure a life-changing student experience.
News and EventsView all
North-East university appoints a new Vice-Chancellor
The University of Sunderland has today announced the appointment of Shirley Atkinson as its new Vice-Chancellor and Chief Executive. The...

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Residence Paradise
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- Swimming pool
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- Pets allowed
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TripAdvisor Reviews Residence Paradise Riva Del Garda
Travel Blogs from Riva Del Garda
Arrivaderci Bella italia
... looking the lake and back down, and down and round and down!!!!! Taking in stunning views, a walk to a hermitage retreat and monestery and a beautiful Romanesque church. Spent the afternoon in the garden, Al Lago before our final dinner in the hotel. Tomorrow, we head off after breakfast , quick stop off in the town of Salo at the bottom of the lake before returning our trusty Italian stallion hire car ( or similar ) and flight home. Now seems as good a time as any to reveal that ...
Rich and Famous
... stay at some cabins in the mountains or not…. The Europeans are so active and fit they put us to shame. Everywhere we go they are walking, running, biking at rapid paces and they are not scared of the odd uphill mountain or two.
Comfort for us we decide and get ready for our splendid evening meal in the dining room. We have dressed up as much as we are able and enter the very quite dining room where the other 20 guests ...
Rest Day
... who frequent the area - and watching all the locals and tourists sunning themselves on the grey pebble beach! It's apparently school holidays in Germany and being so close, Lake Garda is a very popular destination! The place was packed with children and the camping areas full up! Nobody was swimming though as the water is way too cold at present. This area is also very popular ...
Deja vue
... of bands for an outdoor concert tonight on the water-front, and after dinner we went along and had a look see.
Was okay, but after the first act it morphed into almost heavy metal with lots of screeching electric guitars and noise that they consider music.... Interesting....as I type this, at 23.58, it has suddenly stopped....Thank Goodness!
More to the point, where we are at the moment is at the very top of Lake Garda, and ...
Lake Garda
... only some having lake views! The four of us were in that group and we quickly grabbed our keys and headed to our room! Mumsie and I gave a huge sigh of relief when our room had a fantastic view of the little harbour, the mountains and the lake! The room itself was very basic - typical old Italian hotel - but quite comfy and we were certainly very happy! Unfortunately Den and Suze copped a recently ...


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Girls as young as six want to look sexy: Study
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Girls as young as six years are
already beginning to think of themselves as sex objects, a new
study of elementary school-age children in the US has found.
It has been found in the past that women and teens think of themselves in sexually objectified terms, but the new study published in the journal 'Sex Roles' is the first to identify self-sexualisation in young girls.
In the study, psychologists at Knox College in Galesburg, Illionis, used paper dolls to assess self-sexualisation in six- to nine-year-old girls. Sixty girls were shown two dolls, one dressed in tight and revealing "sexy" clothes and the other wearing a trendy but covered-up, loose outfit.
Using a different set of dolls for each question, the team then asked each girl to choose the doll that: looked like herself, looked how she wanted to look, was the popular girl in school, she wanted to play with.
Across-the-board, girls chose the 'sexy' doll most often.
The results were significant in two categories: About 68 per cent of the girls said the doll looked how she wanted to look, and 72 per cent said she was more popular than the non-sexy doll, LiveScience reported.
"It's very possible that girls wanted to look like the sexy doll because they believe sexiness leads to popularity, which comes with many social advantages," said lead researcher
Christy Starr, who was surprised at how many 6- to 7-year old
girls chose the sexualised doll as their ideal self.
Other studies have found that sexiness boosts popularity among girls but not boys. "Although the desire to be popular is not uniquely female, the pressure to be sexy in order to be popular is," she pointed out.
Starr and her team also looked at factors that influenced the girls' responses. Most of the girls were recruited from two public schools, but a smaller subset was recruited from a local dance studio. The girls in this latter group actually chose the non-sexualised doll more often for each of the four questions than did the public-school group.
... contd.


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Wayfair gets with the programmatic ad program
The e-retailer spends at least 50% of its monthly display ad budget on the highly targeted, data-driven—and often cheap—ad placements using programmatic platforms.
Wayfair gets with the programmatic ad program
The e-retailer spends at least 50% of its monthly display ad budget on the highly targeted, data-driven—and often cheap—ad placements using programmatic platforms.
Barnes & Noble will launch a redesigned website
The bookseller aims to reverse declining online sales.
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80% are deploying mobile sites or already have them, with commerce a major driving force.
80% of businesses across various industries, including retail, computer hardware and software, and media, have or are preparing a mobile web site, according to the new Adobe Scene7 Mobile Commerce Survey.
Promotions and commerce are driving these businesses' mobile strategies: 75% of the survey respondents cite promotions as being at the core of their mobile drive, while 62% say commerce.
18% use rich visual merchandising features on their mobile sites or apps, says the survey from Adobe Scene7, a rich media technology provider and a unit of Adobe Systems Inc. These features include alternative images, color swatches/colorizing, zoom/pan and videos.
"Multichannel shoppers tend to purchase more; therefore, companies must effectively engage customers by delivering consistent, rich experiences across all channels, including mobile, to maintain and fuel current double-digit e-commerce industry growth rates," says Sheila Dahlgren, senior director of product marketing at Adobe.
The survey was conducted from July 6 to July 23. Approximately 75% of the 446 respondents were from North America.
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If there's something you've bought offline that you weren't able to buy online, we're probably thinking about that."


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Are you Qing Xiao?Claim your profile
Publications (4)5.29 Total impact
- SourceAvailable from: PubMed Central
Article: SPARC silencing inhibits the growth of acute myeloid leukemia transformed from myelodysplastic syndrome via induction of cell cycle arrest and apoptosis[Show abstract] [Hide abstract]
ABSTRACT: Secreted protein acidic and rich in cysteine (SPARC) plays key roles in erythropoiesis; haploinsufficiency of SPARC is implicated in the progression of the 5q- syndrome. However, the role of SPARC in other subtypes of myelodysplastic syndrome (MDS) is not fully understood, particularly in the del(5q) type with a complex karyotype, which has a high risk to transform into acute myeloid leukemia (AML). In the present study, we investigated the role of SPARC in the proliferation and apoptosis of SKM-1 cells, an acute myeloid leukemia cell line transformed from an MDS cell line. SKM-1 cells were infected with SPARC-RNAi-LV or NC-GFP-LV lentivirus. Apoptosis and cell cycle profiling were assessed by flow cytometry, and cell proliferation was evaluated by MTS assay. The mRNA and protein expression levels of SPARC, p53, caspase-3, caspase-9 and Fas were detected by RT-PCR, real-time PCR and western blot assay. The SPARC shRNA constructed by us led to a significant reduction in SPARC expression in SKM-1 cells. SPARC knockdown inhibited the proliferation of SKM-1 cells by inducing cell cycle arrest at the G1/G0 phase and apoptosis. SPARC knockdown elevated the expression of p53, caspase-9, caspase-3 and Fas at both the mRNA and protein levels. SPARC silencing inhibited the growth of AML transformed from MDS by activating p53-induced apoptosis and cell cycle arrest. These data indicate that SPARC acts as an oncogene in transformed MDS/AML and is a potential therapeutic target in MDS/AML.International Journal of Molecular Medicine 02/2014; 33(4). DOI:10.3892/ijmm.2014.1648 · 1.88 Impact Factor
Article: Ribosomal protein S14 silencing inhibits growth of acute myeloid leukemia transformed from myelodysplastic syndromes via activating p53[Show abstract] [Hide abstract]
ABSTRACT: Ribosomal protein S14 (RPS14) plays a key role in erythropoiesis and causes p53 activation in 5q- syndrome. However, the oncogenic potential of RPS14 is not understood in leukemia and high-risk myelodysplastic syndrome (MDS). Here, we investigated the changes of proliferation and apoptosis of SKM-1, an acute myeloid leukemia (MDS/AML) cell line transformed from MDS, and explored the role of RPS14 in them. SKM-1 cells were transfected with recombined lentiviral vector shRPS14. Reverse-transcribed polymerase chain reaction and western blot assay were carried to detect the expression of RPS14 and p53. Cell proliferation was determined by MTT assay. Cell cycle and apoptosis were detected through flow cytometry. When compared with negative control, the proliferation rate of SKM-1 cells transfected with RPS14 hairpin siRNA dropped by 30%. Transfected SKM-1 cells presented with activation of p53. Transfection also arrested cells in G0/G1 phase and induced apoptosis, indicating that RPS14 is involved in the pathophysiology of MDS/AML. These findings indicate that partial silencing of RPS14 inhibits the proliferation of MDS/AML cells, and RPS14 may negatively regulate p53 activation in MDS/AML cells.Hematology (Amsterdam, Netherlands) 09/2013; 19(4). DOI:10.1179/1607845413Y.0000000127 · 1.19 Impact Factor
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ABSTRACT: Minimal residual disease (MRD) occurrence with some chemotherapy drugs that promote tumor cell escape is also a key factor in blood malignancy relapse. We observed that cytarabine promotes multiple myeloma (MM) cell escape and that the number of cells in the lower chamber increased with increasing clinical disease stage in vitro model which was constructed by a Boyden chamber, matrigel glue and serum from MM patients in different disease stages. The mechanism of cytarabine promotes MM cell escape is closely associated with the up-regulation of CXCR4. SDF-1α can up-regulate the expression of MMP9 and RHoC proteins in MM cells with up-regulated CXCR4, and further promote the cell escape. Fucoidan, a sulfated polysaccharide in the cell wall matrix of brown algae, has attracted much attention for its multiple biological activities, and we further explored the effects and possible underlying mechanisms of fucoidan on MM cell escape from cytarabine cytotoxicity. The results show that fucoidan may decrease MM cell escape from cytarabine cytotoxicity, and that fucoidan can down-regulate CXCR4, MMP9 and RHoC expression. This research provides new direction for investigating MRD occurrence and prevention.Fitoterapia 12/2012; 84. DOI:10.1016/j.fitote.2012.12.018 · 2.22 Impact Factor
Article: [Construction and identification of SPARC shRNA lentiviral vector and its expression in human SKM-1 cells].[Show abstract] [Hide abstract]
ABSTRACT: To construct a lentiviral vector expressing small-hairpin RNA(shRNA) targeting SPARC gene and investigate its silenced effect on SPARC in human myelodysplastic syndromes(MDS) cell line SKM-1. The targeting sequence of SPARC gene which can be effectively silenced in RNA interference was confirmed in our previous study. The designed and synthesized single-stranded primers were annealed to double-stranded oligo sequences and subcloned into linear pGCSIL-GFP lentiviral plasmid digested by enzyme Age I and EcoR I to produce GC-shSPARC lentiviraL vector. After being identified by PCR and sequencing, plasmids GC-shSPARC with pHelper 1.0 and pHelper 2.0 were cotransfected into 293T cells to package lentiviral particles. The recombinant lentiviral vector was transfected into human SKM-1 cells, transfection efficiency was evaluated with expression of green fluorescent protein(GFP) determined by fluorescent microscope. Expression of SPARC in SKM-1 cells was detected using RT-PCR and Western blotting. A recombinant lentiviral vector, GC-shSPARC, expressing shRNAs targeting SPARC gene was constructed and confirmed by DNA sequencing. The recombinant lentivirus was harvested from 293T cells with a viral titer of 1×10(9); TU/mL. GFP was observed in the 70% of SKM-1 cells after transfection. Expression of SPARC mRNA and protein was significantly reduced in the GC-shSPARC transfected group than that in the control group (P<0.05). The lentivirus RNAi vector targeting SPARC has been successfully constructed, and can effectively inhibit the expression of SPARC in SKM-1 cell line, which shed light on the foundation for researching the inhibition of SPARC siRNA target against human MDS cells proliferation, induction apoptosis and gene therapy.Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 05/2012; 28(5):466-9.
Top Journals
Chongqing Medical UniversityCh'ung-ch'ing-shih, Chongqing Shi, China


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Originally written on Fox Sports South | Last updated 4/7/13
ATLANTA Here are three observations from Michigan's 61-56 win over Syracuse in Saturday's second semifinal in Atlanta, a victory that will match the Wolverines up with the Louisville Cardinals in the national championship game: 1. Syracuse did not have the mismatches it maybe thought it did During Friday's breakout sessions with players from all four teams, Syracuse guard Brandon Triche could be heard saying his team could exploit mismatches at every position on the floor against Michigan Well, Mitch McGary proved to be a mismatch for Syracuse's zone. The freshman has been one of the best players throughout the NCAA tournament his performances against VCU and Kansas particularly stand out and he was indeed a problem for coach Jim Boeheim's 2-3 zone. By letting McGary roam around the free throw line and then attack the soft spots in the zone, either by driving the ball to the hoop or passing, he was able to help the Wolverines consistently find points against the fearsome Sy...
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Enacted — Signed by the President — Jun 29, 2015
H.R. 2146: Defending Public Safety Employees' Retirement Act
Last Action: Signed by President.
Explanation: This bill was enacted after being signed by the President on June 29, 2015.
Mar 30, 2006
109th Congress, 2005–2006
This resolution was introduced on March 30, 2006, in a previous session of Congress, but was not enacted.
Representative for Pennsylvania's 7th congressional district
Read Text »
Last Updated: Mar 30, 2006
Length: 2 pages
The "H.Con.Res." in "H.Con.Res. 375" means this is a House concurrent resolution in the United States Congress. A concurrent resolution is often used for matters that affect the rules of Congress or to express the sentiment of Congress. It must be agreed to by both the House and Senate in identical form but is not signed by the President and does not carry the force of law.
The resolution's title was written by its sponsor.
The committee chair determines whether a resolution will move past the committee stage.
There have been no votes related to this resolution.
Search for similar bills.
THOMAS.gov (The Library of Congress)
GovTrack gets most information from THOMAS, which is updated generally one day after events occur. Activity since the last update may not be reflected here. Data comes via the congress project.
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Civic Impulse. (2015). H.Con.Res. 375 — 109th Congress: Expressing the sense of Congress to support the decision of the United States Court of .... Retrieved from https://www.govtrack.us/congress/bills/109/hconres375
"H.Con.Res. 375 — 109th Congress: Expressing the sense of Congress to support the decision of the United States Court of ...." www.GovTrack.us. 2006. July 4, 2015 <https://www.govtrack.us/congress/bills/109/hconres375>
{{cite web
|title=H.Con.Res. 375 (109th)
|accessdate=July 4, 2015
|author=109th Congress (2006)
|date=March 30, 2006
|quote=Expressing the sense of Congress to support the decision of the United States Court of ...


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From Milwaukee, Madison and beyond, a daily dose of political news and glimpses behind the scenes
Campaign watchdog fires back at Gableman
Madison -- The debate over a letter from state Supreme Court candidate Michael Gableman's campaign rages on.
Let's back up. On Tuesday, the Wisconsin Judicial Campaign Integrity Committee said it didn't think any further action was required on a letter sent by law enforcement officials on behalf of Gableman, a Burnett County circuit court judge. The letter criticized incumbent Justice Louis Butler's participation in decisions on criminal defense cases. One Wisconsin Now, a group that brings together those on the left side of the political spectrum, last week asked the committee to review the letter, saying it could lead voters to believe Gableman would be the deciding vote to overturn the decisions if similar cases reach the court.
Sun Prairie attorney Charlie Schutze is also in the race.
After the committee said further review wasn't necessary, Gableman's campaign put out a statement in which campaign consultant Darrin Schmitz said Butler "cannot hide the fact that he consistently sides with criminals over law enforcement."
That went too far for the Judicial Campaign Integrity Committee. In a letter to Gableman today, committee chair Tom Basting, head of the State Bar of Wisconsin, said Schmitz's comment was "precisely the kind of campaign rhetoric" the committee hoped it wouldn't hear or read and asked Gableman to repudiate it.
"As you know, in our system of government, judges do not 'side' with or against any of the parties to legal proceedings who may appear in their courtrooms," the letter said. Characterizing a justice that way would create the impression that Wisconsin judges take sides, it continued.
In a statement responding to today's letter, Schmitz pointed to the part that said prior court rulings are fair game in the race.
"We're pleased that WJCIC committee agreed with us in that Louis Butler's 'court rulings are clearly within bounds for discussion,'" Schmitz said. "That is how we have conducted ourselves thus far and will continue to do so as the campaign unfolds."
The letter from the Judicial Campaign Integrity Committee is attached below.
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Town Square
Fire destroys Woodside home
Original post made on Jun 4, 2012
Read the full story here Web Link posted Sunday, June 3, 2012, 4:34 PM
Comments (3)
a resident of Palo Verde
on Jun 4, 2012 at 10:55 am
Its a long way up Bear Gulch Road. I viewed the smoke from the Baylands; the fire location was nearly up to Skyline. I am amazed that this fire did not turn into something much larger. Kudos to the responders. However, I did't see any air support. Maybe they had departed by the time I saw the smoke.
a resident of Atherton
on Jun 4, 2012 at 1:56 pm
Peter Carpenter is a registered user.
See the Almanac story:
Web Link
Water from a helicopter key in preventing conflagration this weekend
by Dave Boyce
Almanac Staff
Three 500-gallon buckets of water from a helicopter helped douse a three-alarm house fire at 1200 Bear Gulch Road on Saturday, June 2. The helicopter drops stopped an intense fire that had spread to nearby trees from becoming a conflagration that involved the forest, Fire Chief Dan Ghiorso of the Woodside Fire Protection District told the Almanac.
a resident of Midtown
on Jun 4, 2012 at 2:25 pm
Hope no pets were harmed by the fire either.
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Are you Peng-Yuan Yang?Claim your profile
Publications (89)245.11 Total impact
Article: Solving signal instability to maintain the second-order advantage in the resolution and determination of multi-analytes in complex systems by modeling liquid chromatography-mass spectrometry data using alternating trilinear decomposition method assisted with piecewise direct standardization[Show abstract] [Hide abstract]
ABSTRACT: The application of calibration transfer methods has been successful in combination with near-infrared spectroscopy or other tools for prediction of chemical composition. One of the developed methods that can provide accurate performances is the piecewise direct standardization (PDS) method, which in this paper is firstly applied to transfer from one day to another the second-order calibration model based on alternating trilinear decomposition (ATLD) method built for the interference-free resolution and determination of multi-analytes in complex systems by liquid chromatography-mass spectrometry (LC-MS) in full scan mode. This is an example of LC-MS analysis in which interferences have been found, making necessary the use of second-order calibration because of its capacity for modeling this phenomenon, which implies analytes of interest can be resolved and quantified even in the presence of overlapped peaks and unknown interferences. Once the second-order calibration model based on ATLD method was built, the calibration transfer was conducted to compensate for the signal instability of LC-MS instrument over time. This allows one to reduce the volume of the heavy works for complete recalibration which is necessary for later accurate determinations. The root-mean-square error of prediction (RMSEP) and average recovery were used to evaluate the performances of the proposed strategy. Results showed that the number of calibration samples used on the real LC-MS data was reduced by using the PDS method from 11 to 3 while producing comparable RMSEP values and recovery values that were statistically the same (F-test, 95% confidence level) to those obtained with 11 calibration samples. This methodology is in accordance with the highly recommended green analytical chemistry principles, since it can reduce the experimental efforts and cost with regard to the use of a new calibration model built in modified conditions. Copyright © 2015 Elsevier B.V. All rights reserved.Journal of Chromatography A 06/2015; DOI:10.1016/j.chroma.2015.06.049 · 4.26 Impact Factor
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ABSTRACT: Colorectal cancer (CRC) is the third and second most common cancer in males and females worldwide, respectively. Spondin-2 is a conserved secreted extracellular matrix protein and a candidate cancer biomarker. Here we found that Spondin-2 mRNA was upregulated in CRC tissues using quantitative RT-PCR and data-mining of public Oncomine microarray datasets. Spondin-2 protein was increased in CRC tissues, as revealed by immunohistochemistry analyses of two tissue microarrays containing 180 cases. Spondin-2 gene expression was significantly associated with CRC stage, T stage, M stage and Dukes stage, while its protein was associated with age and M stage. Kaplan-Meier analysis revealed that the upregulated Spondin-2 mRNA and protein predicted poor prognosis of CRC patients. Univariate and multivariate Cox regression analyses indicated that grade, recurrence, N stage and high Spondin-2 were independent predictors of overall survival of CRC patients. ELISA revealed that plasma Spondin-2 was upregulated in CRC and dropped after surgery. Receiver operating characteristic curve analysis demonstrated that plasma Spondin-2 has superior predictive performance for CRC with an area under the curve of 0.959 and the best sensitivity/specificity of 100%/90%. Furthermore, ectopic expression of Spondin-2 enhanced colon cancer cell proliferation. Spondin-2 could be an independent diagnostic and prognostic biomarker of colon cancer.Oncotarget 04/2015; · 6.63 Impact Factor
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ABSTRACT: The tumor cell proliferation, migration and invasion were influenced by the interaction between the cancer cells and their microenvironment. In current study, we established two pairs of the primary fibroblast cultures from colorectal adenocarcinoma tissues and the normal counterparts and identified 227 proteins in the colonic fibroblast secretomes; half of these proteins were novel. The mass spectrometry data and analyzed results presented here provide novel insights into the molecular characteristics and modulatory role of colon cancer associated fibroblasts. The data is related to "Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation" by Chen et al. [1].12/2014; 1:19-24. DOI:10.1016/j.dib.2014.08.003
Article: Filamin C, a dysregulated protein in cancer revealed by label-free quantitative proteomic analyses of human gastric cancer cells.[Show abstract] [Hide abstract]
ABSTRACT: Gastric cancer (GC) is the fourth and fifth most common cancer in men and women, respectively. We identified 2,750 proteins at false discovery rates of 1.3% (protein) and 0.03% (spectrum) by comparing the proteomic profiles of three GC and a normal gastric cell lines. Nine proteins were significantly dysregulated in all three GC cell lines, including filamin C, a muscle-specific filamin and a large actin-cross-linking protein. Downregulation of filamin C in GC cell lines and tissues were verified using quantitative PCR and immunohistochemistry. Data-mining using public microarray datasets shown that filamin C was significantly reduced in many human primary and metastasis cancers. Transient expression or silencing of filamin C affected the proliferation and colony formation of cancer cells. Silencing of endogenous filamin C enhanced cancer cell migration and invasion, whereas ectopic expression of filamin C had opposing effects. Silencing of filamin C increased the expression of matrix metallopeptidase 2 and improved the metastasis of prostate cancer in a zebrafish model. High filamin C associated with better prognosis of prostate cancer, leukemia and breast cancer patients. These findings establish a functional role of filamin C in human cancers and these data will be valuable for further study of its mechanisms.Oncotarget 11/2014; · 6.63 Impact Factor
Article: Multi-targeted interference-free determination of ten β-blockers in human urine and plasma samples by alternating trilinear decomposition algorithm-assisted liquid chromatography–mass spectrometry in full scan mode: Comparison with multiple reaction monitoring[Show abstract] [Hide abstract]
ABSTRACT: β-blockers are the first-line therapeutic agents for treating cardiovascular diseases and also a class of prohibited substances in athletic competitions. In this work, a smart strategy that combines three-way liquid chromatography-mass spectrometry (LC-MS) data with second-order calibration method based on alternating trilinear decomposition (ATLD) algorithm was developed for simultaneous determination of ten β-blockers in human urine and plasma samples. This flexible strategy proved to be a useful tool to solve the problems of overlapped peaks and uncalibrated interferences encountered in quantitative LC-MS, and made the multi-targeted interference-free qualitative and quantitative analysis of β-blockers in complex matrices possible. The limits of detection were in the range of 2.0×10(-5)-6.2×10(-3)μgmL(-1), and the average recoveries were between 90 and 110% with standard deviations and average relative prediction errors less than 10%, indicating that the strategy could provide satisfactory prediction results for ten β-blockers in human urine and plasma samples only using liquid chromatography hyphenated single-quadrupole mass spectrometer in full scan mode. To further confirm the feasibility and reliability of the proposed method, the same batch samples were analyzed by multiple reaction monitoring (MRM) method. T-test demonstrated that there are no significant differences between the prediction results of the two methods. Considering the advantages of fast, low-cost, high sensitivity, and no need of complicated chromatographic and tandem mass spectrometric conditions optimization, the proposed strategy is expected to be extended as an attractive alternative method to quantify analyte(s) of interest in complex systems such as cells, biological fluids, food, environment, pharmaceuticals and other complex samples.Analytica Chimica Acta 10/2014; 848:10–24. DOI:10.1016/j.aca.2014.08.052 · 4.52 Impact Factor
Article: Identification of colonic fibroblast secretomes reveals secretory factors regulating colon cancer cell proliferation[Show abstract] [Hide abstract]
ABSTRACT: Stromal microenvironment influences tumor cell proliferation and migration. Fibroblasts represent the most abundant stromal constituents. Here, we established two pairs of normal fibroblast (NF) and cancer-associated fibroblast (CAF) cultures from colorectal adenocarcinoma tissues and the normal counterparts. The NFs and CAFs were stained positive for typical fibroblast markers and inhibited colon cancer (CC) cell proliferation in in vitro cocultures and in xenograft mouse models. The fibroblast conditioned media were analyzed using LC-MS and 227 proteins were identified at a false discovery rate of 1.3%, including 131 putative secretory and 20 plasma membrane proteins. These proteins were enriched for functional categories of extracellular matrix, adhesion, cell motion, inflammatory response, redox homeostasis and peptidase inhibitor. Secreted protein acidic and rich in cysteine, transgelin, follistatin-related protein 1 (FSTL1) and decorin were abundant in the fibroblast secretome as confirmed by Western blot. Silencing of FSTL1 and transgelin in colonic fibroblast cell line CCD-18Co induced an accelerated proliferation of CC cells in cocultures. Exogenous FSTL1 attenuates CC cell proliferation in a negative fashion. FSTL1 was upregulated in CC patient plasma and cancerous tissues but had no implication in prognosis. Our results provided novel insights into the molecular signatures and modulatory role of CC associated fibroblasts.Journal of Proteomics 08/2014; 110. DOI:10.1016/j.jprot.2014.07.031 · 3.93 Impact Factor
Article: Global in vivo terminal amino acid labeling for exploring differential expressed proteins induced by dialyzed serum cultivation[Show abstract] [Hide abstract]
ABSTRACT: Taking advantage of reliable metabolic labeling and accurate isobaric MS2 quantification, we developed a global in vivo terminal amino acid labeling (G-IVTAL) strategy by combining metabolic labeling and isotopic dimethyl labeling for quantifying tryptic peptides. With G-IVTAL, the scale of qualitative and quantitative data can be increased twofold compared with in vivo termini amino acid labeling (IVTAL) in which Lys-N and Arg-C are used for digestion. As a result, up to 81.78% of the identified proteins have been confidently quantified in G-IVTAL-labeled HepG2 cells. Dialyzed serum has been used in most SILAC studies to ensure complete labeling. However, dialysis requires the removal of low molecular weight hormones, cytokines, and cellular growth factors, which are essential for the cell growth of certain cell lines. To address the influence of dialyzed serum in HepG2 growth, the G-IVTAL strategy was applied to quantify the expression differences between dialyzed serum- and normal serum-cultured HepG2 cells. Finally, we discovered 111 differentially expressed proteins, which could be used as references to improve the reliability of the SILAC quantification. Among these, by using western blotting, the differential expressions of MTDH, BCAP31, and GPC3 were confirmed as being influenced by dialyzed serum. The experimental results demonstrate that the G-IVTAL strategy is a powerful tool to achieve accurate and reliable protein quantification.The Analyst 07/2014; 139(18). DOI:10.1039/c4an00728j · 3.91 Impact Factor
Article: Analysis of Rat Liver Proteins by Peptide Immobilized pH Gradient Isoelectric Focusing Combined with Liquid Chromatography-Tandem Mass Spectrometry[Show abstract] [Hide abstract]
ABSTRACT: Due to the complexity of the biological sample, it is important to develop an effective pre-separation method for the liquid chromatography-mass spectrometer (LC-MS) analysis of biological samples. In this experiment, enzymatic hydrolysate of rat liver protein was pre-separated by peptide immobilized pH gradient isoelectric focusing (IPG-IEF) method, and the pre-separated components were further separated by reversed physe liquid chromatography (RPLC) and then identified by LTQ-Orbitrap MS. A total of 2039 kinds of proteins were identified, including 18 kinds of acetylated proteins, 4 kinds of proteins which were never reported before. Next, bioinformatic analysis was carried out for these proteins. The results showed that peptide IPG-IEF coupled with LC-MS/MS is an effective technique for proteomics analysis and this method is suitable for large-scale protein identification.Chinese Journal of Analytical Chemistry 02/2014; 42(2):203–208. DOI:10.1016/S1872-2040(13)60710-0 · 0.79 Impact Factor
Article: Reduced expression of the chromatin remodelling gene ARID1A enhances gastric cancer cell migration and invasion via downregulation of E-cadherin transcription.[Show abstract] [Hide abstract]
ABSTRACT: The chromatin remodeling gene AT-rich interactive domain-containing protein 1A (ARID1A) encodes the protein BAF250a, a subunit of human SWI/SNF-related complexes. Recent studies have identified ARID1A as a tumor suppressor. Here, we show that ARID1A expression is reduced in gastric cancer (GC) tissues, which are significantly associated with local lymph node metastasis, tumor infiltration and poor patient prognosis. ARID1A silencing enforces the migration and invasion of GC cells, whereas ectopic expression of ARID1A inhibits migration. The adhesive protein E-cadherin is remarkably downregulated in response to ARID1A silencing, but it is upregulated by ARID1A overexpression. E-cadherin overexpression significantly inhibits GC cell migration and invasion, while CDH1 (coded E-cadherin) silencing promotes migration. Restored expression of CDH1 in ARID1A-silenced cell lines restores the inhibition of cell migration. Luciferase reporter assays and chromatin immunoprecipitation indicate that the ARID1A-associated SWI/SNF complex binds to the CDH1 promoter and modulates CDH1 transcription. ARID1A knockdown induces evident morphological changes of GC cells with increased expression of mesenchymal markers, indicating an epithelial-mesenchymal transition. ARID1A silencing does not alter the level of β-catenin, but induces a subcellular redistribution of β-catenin from the plasma membrane to the cytoplasm and nucleus. Immunohistochemical studies demonstrate that reduced expression of E-cadherin is associated with local lymph node metastasis, tumor infiltration and poor clinical prognosis. ARID1A and E-cadherin expression show a strong correlation in 75.4% of the analyzed GC tissues. They are synergistically downregulated in 23.5% of analyzed GC tissues. In conclusion, ARID1A targets E-cadherin during the modulation of GC cell migration and invasion.Carcinogenesis 11/2013; DOI:10.1093/carcin/bgt398 · 5.27 Impact Factor
Article: iTRAQ-based quantitative proteomics reveals myoferlin as a novel prognostic predictor in pancreatic adenocarcinoma[Show abstract] [Hide abstract]
ABSTRACT: Histological differentiation is a major pathological parameter associated with poor prognosis in patients with pancreatic adenocarcinoma (PAC) and the molecular signature underlying PAC differentiation may involve key proteins potentially affecting the malignant characters of PAC. We aimed to identify the proteins which could be implicated in PAC prognosis. We used isobaric tags for relative and absolute quantitation (iTRAQ) coupled with two-dimensional liquid chromatography-tandem mass spectrometry to compare protein expression in PAC tissues with different degree of histological differentiation. A total of 1623 proteins were repeatedly identified by performing the iTRAQ-based experiments twice. Of these, 15 proteins were differentially expressed according to our defined criteria. Myoferlin(MYOF) was selected to validate the proteomic results by western blotting. Immunohistochemistry in a further 154 PAC cases revealed that myoferlin significantly correlated with the degree of histological differentiation (P=0.004), and univariate and multivariate analyses indicated that MYOF is an independent prognostic factor for survival (hazard ratio, 1.540; 95% confidence interval, 1.061-2.234; P=0.023) of patients with PAC after curative surgery. RNA interference-mediated knockdown of MYOF alleviated malignant phenotypes of both primary and metastatic PAC cell lines in vitro and in vivo. Thus, ITRAQ-based quantitative proteomics revealed the prognostic value of MYOF in PAC. our results provide the possibility of novel strategies for pancreatic adenocarcinoma management.Journal of proteomics 07/2013; 91. DOI:10.1016/j.jprot.2013.06.032 · 3.93 Impact Factor
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ABSTRACT: A freezing technique protocol was proposed for coupling microchip electrophoresis with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS). The microfluidic flow was frozen immediately after electrophoresis on microfluidic chip and the separated analyte molecules were kept in their zone pattern in the electrophoresis. Then, the frozen-chip was lyophilized and sent into TOF-MS instrument as a MALDI target, and the analyte molecules in the microfluidic channels were subjected to analysis by mass spectrometry. This approach could eliminate sample cross-contamination, providing a new interface for microchip electrophoresis and MALDI-MS.Chinese Chemical Letters 06/2013; 24(6):491–493. DOI:10.1016/j.cclet.2013.03.053 · 1.18 Impact Factor
Article: A miniaturised electron ionisation time-of-flight mass spectrometer that uses a unique helium ion removal pulsing technique specifically for gas analysis[Show abstract] [Hide abstract]
ABSTRACT: A miniaturised reflectron time-of-flight mass spectrometer combined with an electron ionisation ion source has been developed for the analysis of gases. An entirely new helium ion removal pulsing technique in this mass spectrometer is used to achieve an improved performance for the first time. The helium carrier gas, which enters into the source along with the gaseous sample, is simultaneously ionised and then orthogonally introduced into the time-of-fight mass analyser. Once the relatively light helium ions in the ion packet become extremely close to the reflectron plate (B-plate for short in this article), a modulated pulse is instantaneously applied on the B-plate and a negative reflectron voltage is set to the B-plate and lasts for a very short period, during which all the helium ions are directly bumped into the B-plate and subsequently removed. The helium ion removal pulsing technique can efficiently avoid saturation of the micro-channel plate caused by too many helium ions. A compact and durable instrument is designed, which has a mass resolving resolution greater than 400 FWHM for online gas analysis. The technology may also be further developed to remove other ions for TOF mass spectrometry.The Analyst 05/2013; 138(12). DOI:10.1039/c3an00217a · 3.91 Impact Factor
Article: Proteomic characterization of larval and adult developmental stages in Echinococcus granulosus reveals novel insight into host–parasite interactions[Show abstract] [Hide abstract]
ABSTRACT: Cystic hydatid disease is an important zoonosis caused by Echinococcus granulosus infection. The expression profiles of its parasitic life stages and host-Echinococcus interactions remain to be elucidated. Here, we identified 157 adult and 1588 protoscolex proteins (1610 in all), including 1290 novel identifications. Paramyosins and an antigen B (AgB) were the dominant adult proteins. Dog proteins (30) identified in adults indicated diminished local inflammation caused by adult infection. The protoscolex expresses proteins that have been reported to be antigens in other parasites, such as 6-phosphofructokinase and calcineurin B. Pathway analyses suggested that E. granulosus uses both aerobic and anaerobic carbohydrate metabolisms to generate ATP. E. granulosus expresses proteins involved in synthesis and metabolism of lipids or steroids. At least 339 of 390 sheep proteins identified in protoscolex were novel identifications not seen in previous analyses. IgGs and lambda light chains were the most abundant antibody species. Sheep proteins were enriched for detoxification pathways, implying that host detoxification effects play a central role during host-parasite interactions. Our study provides valuable data on E. granulosus expression characteristics, allowing novel insights into the molecular mechanisms involved in host-parasite interactions.Journal of proteomics 04/2013; 84. DOI:10.1016/j.jprot.2013.04.013 · 3.93 Impact Factor
Article: Identification of proteins implicated in the development of pancreatic cancer-associated diabetes mellitus by iTRAQ-based quantitative proteomics[Show abstract] [Hide abstract]
ABSTRACT: Studies have revealed that pancreatic cancer (PC) may lead to diabetes mellitus (DM). We aimed to identify the proteins implicated in the development of PC-associated DM in PC tissues with DM.We used isobaric tags for relative and absolute quantitation (iTRAQ) coupled with two-dimensional liquid chromatography-tandem mass spectrometry to compare protein expression in PC tissues with DM with that in PC tissues without DM and in adjacent nontumor tissues with or without DM. A total of 80 surgically resected fresh tissues from 40 PC patients were included to identify differential protein expression. Western blotting and immunohistochemistry were then applied to evaluate the differential expression of selected proteins.A total of 1611 proteins were repeatedly identified and quantified by performing the iTRAQ-based experiments twice. Of these, 23 proteins were differentially expressed according to our defined criteria (12 upregulated and 11 downregulated). The S100 calcium binding protein A9 and aldehyde dehydrogenase 2 family were selected to validate the proteomic results by western blotting and immunohistochemistry.The identification of key proteins implicated in the development of PC-associated DM could serve as a foundation to better understand and further explore the etiology and pathogenesis of PC-associated DM. BIOLOGICAL SIGNIFICANCE: The identification of key proteins implicated in the development of PC-associated DM could serve as a foundation to better understand and further explore the etiology and pathogenesis of PC-associated DM.Journal of proteomics 04/2013; 84. DOI:10.1016/j.jprot.2013.03.031 · 3.93 Impact Factor
Article: Comparative proteomic analysis of drug sodium iron chlorophyllin addition to Hep 3B cell line.[Show abstract] [Hide abstract]
ABSTRACT: The human hepatoma 3B cell line was chosen as an experimental model for in vitro test of drug screening. The drugs included chlorophyllin and its derivatives such as fluo-chlorophyllin, sodium copper chlorophyllin, and sodium iron chlorophyllin. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide (MTT) method was used in this study to obtain the primary screening results. The results showed that sodium iron chlorophyllin had the best LC(50) value. Proteomic analysis was then performed for further investigation of the effect of sodium iron chlorophyllin addition to the Hep 3B cell line. The proteins identified from a total protein extract of Hep 3B before and after the drug addition were compared by two-dimensional-gel-electrophoresis. Then 32 three-fold differentially expressed proteins were successfully identified by MALDI-TOF-TOF-MS. There are 29 unique proteins among those identified proteins. These proteins include proliferating cell nuclear antigen (PCNA), T-complex protein, heterogeneous nuclear protein, nucleophosmin, heat shock protein A5 (HspA5) and peroxiredoxin. HspA5 is one of the proteins which are involved in protecting cancer cells against stress-induced apoptosis in cultured cells, protecting them against apoptosis through various mechanisms. Peroxiredoxin has anti-oxidant function and is related to cell proliferation, and signal transduction. It can protect the oxidation of other proteins. Peroxiredoxin has a close relationship with cancer and can eventually become a disease biomarker. This might help to develop a novel treatment method for carcinoma cancer.The Analyst 08/2012; 137(18):4287-94. DOI:10.1039/c2an35436e · 3.91 Impact Factor
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ABSTRACT: Electron transfer dissociation (ETD) is a useful and complementary activation method for peptide fragmentation in mass spectrometry. However, ETD spectra typically receive a relatively low score in the identifications of 2+ ions. To overcome this challenge, we, for the first time, systematically interrogated the benefits of combining ion charge enhancing methods (dimethylation, guanidination, m-nitrobenzyl alcohol (m-NBA) or Lys-C digestion) and differential search algorithms (Mascot, Sequest, OMSSA, pFind and X!Tandem). A simple sample (BSA) and a complex sample (AMJ2 cell lysate) were selected in benchmark tests. Clearly distinct outcomes were observed through different experimental protocol. In the analysis of AMJ2 cell lines, X!Tandem and pFind revealed 92.65% of identified spectra; m-NBA adduction led to a 5-10% increase in average charge state and the most significant increase in the number of successful identifications, and Lys-C treatment generated peptides carrying mostly triple charges. Based on the complementary identification results, we suggest that a combination of m-NBA and Lys-C strategies accompanied by X!Tandem and pFind can greatly improve ETD identification.Molecular BioSystems 07/2012; 8(10):2692-8. DOI:10.1039/c2mb25106j · 3.18 Impact Factor
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ABSTRACT: Quantification by series of b, y fragment ion pairs generated from isobaric-labeled peptides in MS2 spectra has recently been considered an accurate strategy in quantitative proteomics. Here we developed a novel MS2 quantification approach named quantitation by isobaric terminal labeling (QITL) by coupling (18)O labeling with dimethylation. Trypsin-digested peptides were labeled with two (16)O or (18)O atoms at their C-termini in H(2)(16)O or H(2)(18)O. After blocking all ε-amino groups of lysines through guanidination, the N-termini of the peptides were accordingly labeled with formaldehyde-d(2) or formaldehyde. These indistinguishable, isobaric-labeled peptides in MS1 spectra produce b, y fragment ion pairs in the whole mass range of MS2 spectra that can be used for quantification. In this study, the feasibility of QITL was first demonstrated using standard proteins. An accurate and reproducible quantification over a wide dynamic range was achieved. Then, complex rat liver samples were used to verify the applicability of QITL for large-scale quantitative analysis. Finally, QITL was applied to profile the quantitative proteome of hepatocellular carcinoma (HCC) and adjacent non-tumor liver tissues. Given its simplicity, low-cost, and accuracy, QITL can be widely applied in biological samples (cell lines, tissues, and body fluids, etc.) for quantitative proteomic research.Journal of proteomics 07/2012; 75(18):5797-806. DOI:10.1016/j.jprot.2012.07.011 · 3.93 Impact Factor
Article: Determination of Cr, Ni, Cu, Mn, P, Si, Mo and Ti in High Chromium Cast Iron by Inductively Coupled Plasma Atomic Emission Spectrometry[Show abstract] [Hide abstract]
ABSTRACT: The high-chromium cast iron sample was microwave-assisted digested with aqueous regia in a closed vessel. Series standards were prepared with matching Fe matrix and adding Y as internal standard. Line intensities of the prepared standards and the digested sample solutions were determined by inductively coupled plasma atomic emission spectrometry. Accuracy of the proposed method was verified by the analysis of three national standard Materials GSBH 41018, GBW 01120 and GBW 01121, and the results were well agreed with the certification data.Guang pu xue yu guang pu fen xi = Guang pu 09/2011; 31(9):2558-60. DOI:10.3964/j.issn.1000-0593(2011)09-2558-03 · 0.27 Impact Factor
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ABSTRACT: Quantitative proteomics is one of the research hotspots in the proteomics field and presently maturing rapidly into an important branch. The two most typical quantitative methods, stable isotope labeling with amino acids in cell culture (SILAC) and isobaric tags for relative and absolute quantification (iTRAQ), have been widely and effectively applied in solving various biological and medical problems. Here, we describe a novel quantitative strategy, termed "IVTAL", for in vivo termini amino acid labeling, which combines some advantages of the two methods above. The core of this strategy is a set of heavy amino acid (13)C(6)-arginine and (13)C(6)-lysine and specific endoproteinase Lys-N and Arg-C that yield some labeled isobaric peptides by cell culture and enzymatic digestion, which are indistinguishable in the MS scan but exhibit multiple MS/MS reporter b, y ion pairs in a full mass range that support quantitation. Relative quantification of cell states can be achieved by calculating the intensity ratio of the corresponding reporter b, y ions in the MS/MS scan. The experimental analysis for various proportions of mixed HeLa cell samples indicated that the novel strategy showed an abundance of reliable quantitative information, a high sensitivity, and a good dynamic range of nearly 2 orders of magnitude. IVTAL, as a highly accurate and reliable quantitative proteomic approach, is expected to be compatible with any cell culture system and to be especially effective for the analysis of multiple post-translational modificational sites in one peptide.Analytical Chemistry 06/2011; 83(15):6026-33. DOI:10.1021/ac201035f · 5.83 Impact Factor
Article: iTRAQ-2DLC-ESI-MS/MS Based Identification of a New Set of Immunohistochemical Biomarkers for Classification of Dysplastic Nodules and Small Hepatocellular Carcinoma[Show abstract] [Hide abstract]
ABSTRACT: The study aims to develop novel clinical immunohistochemical biomarkers for distinguishing small hepatocellular carcinoma (sHCC) from dysplastic nodules (DN). iTRAQ-2DLC-ESI-MS/MS technique was used to screen immunohistochemical biomarkers between precancerous lesions (liver cirrhosis and DN) and sHCC. A total of 1951 proteins were quantified, including 52 proteins upregulated in sHCC and 95 proteins downregulated in sHCC by at least 1.25- or 0.8-fold at p < 0.05. The selected biomarker candidates were further verified using Western blotting and immunohistochemistry. Furthermore, receiver operation characteristics (ROC) curves and logistic regression model were carried out to evaluate the diagnostic values of the biomarkers. Finally, aminoacylase-1 (ACY1) and sequestosome-1 (SQSTM1) were chosen as novel candidate biomarkers for distinction of sHCC from DN. A constructed logistic regression model included ACY1, SQSTM1, and CD34. The sensitivity and specificity of this model for distinguishing sHCC from DN was 96.1% and 96.7%. In conclusion, ACY1 and SQSTM1 were identified as novel immunohistochemical biomarkers distinguishing sHCC from DN. In conclusion, expression levels of CD34, ACY1, and SQSTM1 can be used to establish an accurate diagnostic model for distinction of sHCC from DN.Journal of Proteome Research 06/2011; 10(8):3418-28. DOI:10.1021/pr200482t · 5.00 Impact Factor
Top Journals
Fudan University
Shanghai, Shanghai Shi, China
- • Institutes of Biomedical Sciences
- • Department of Chemistry
Second Military Medical University, Shanghai
Shanghai, Shanghai Shi, China
- International Cooperation Laboratory on Signal Transduction
Changhai Hospital, ShanghaiShanghai, Shanghai Shi, China
Chinese National Human Genome Center at ShanghaiShanghai, Shanghai Shi, China
Chinese Academy of SciencesPeping, Beijing, China


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PROCESSES AND THREADS Threads, and processes, and tasks! Oh, my! Process – linux process Usually one process per application Thread – linux thread May be multiple per process May encompass multiple application components Tasks Android stack of activities May cross process boundaries One task stack for each "job" Task stack Stack of activities rooted at initial activity Multiple tasks may exists at once Background task stacks Back button pops activity from stack Processes When an application component is launched, if no other component is running, a new process with single thread is started Separate process can be specified for component(s) using android:process attribute in manifest file Processes may be killed due to low memory importance hierarchy from process lifecycle Threads When application is launched, creates "main" thread UI thread Long operations should go in extra threads Background / worker threads Single-threaded model for UI 2 rules Do not block UI thread Do not access the android UI toolkit from outside the UI thread UI toolkit not thread-safe, must always be manipulated in UI thread Several ways to access UI thread from extra threads Activity.runOnUiThread(Runnable) View.post(Runnable) View.postDelayed(Runnable, long) Handler Threads Threads and Runnables created using standard Java syntax Example new thread creation new Thread (new Runnable() { public void run() { // implementation . . . } }).start(); UI helper thread example public void onClick (View v) { new Thread(new Runnable() { public void run() { final Bitmap bitmap = loadImageFromNetwork ("http://exmpl.com/img.png"); mImageView.post (new Runnable() { public void run() { mImageView.setImageBitmap(bitmap); } }); } }).start(); } Looper Android class for providing message queue for threads UI thread has a Looper created for it implicity Can connect to this queue and handle messages by declaring new handler in main thread HandlerThread Handy class for starting a new thread that has a looper Handler Handles messages and runnables passed to a thread's message queue Connects to thread's Looper Thread safe Extend Handler and override handleMessage(Message msg) Message Members public int what public int arg1 public int arg2 public object obj public Messenger replyTo Typically constructed using Message.obtain() returns message object from global pool to avoid allocating new objects Handler Example implementation class MyHandler extends Handler { @Override public void handleMessage (Message msg) { switch (msg.what) { case 1: // do something; break; case 2: // do something else; break; default: super.handleMessage(msg); } } } Looper/Handler example AsyncTask Simplifies creation of long-running tasks that need to communicate with the UI Must be subclassed Instance must be created on UI thread Instance can only be executed once Automatically invokes doInBackground() on worker thread onPreExecute(), onPostExecute(), onProgressUpdate() on UI thread Return value of doInBackground() passed to onPostExecute() publishProgress() can be called in doInBackground() to execute onProgressUpdate() Useful for progress bar updates AsyncTask example public void onClick(View v) { new DownloadImageTask().execute("http://exmpl.com/img.png"); } private class DownloadImageTask extends AsyncTask<String, Void, Bitmap> { protected Bitmap doInBackground(String... urls) { return loadImageFromNetwork(urls[0]); } protected void onPostExecute(Bitmap result) { mImageView.setImageBitmap(result); } } Interprocess communication Remote procedure calls can be accomplished two ways Android interface definition language (AIDL) Messenger service AIDL provides custom defined interface Requires other applications to have AIDL files Messenger service has standard format but less flexible Binder of AIDL or Messenger interface can be returned to clients in onBind() AIDL Defines an interface for interprocess communication Needs to be thread-safe Calls from local process are handled in caller thread Calls from remote process are handled from thread pool Calls to interface are direct function calls (synchronous), unless oneway keyword specified (asynchronous) Interface define in .aidl file Android SDK tools automatically generate IBinder interface Example .aidl file // IRemoteService.aidl package com.example.android; // Declare any non-default types here with import statements /** Example service interface */ interface IRemoteService { /** Request the process ID of this service */ int getPid(); /** Demonstrates some basic types that you can use as * parameters and return values in AIDL. */ void basicTypes(int anInt, long aLong, boolean aBoolean, float aFloat, double aDouble, String aString); } Messenger Pointer to Handler Allows handler to be called from other processes Can be used for interprocess message passing To expose handler Initialize Messenger with Handler to share Messenger mMessenger = new Messenger (new MyHandler()); To connect to remote handler Initialize Messenger with IBinder of remote interface Messenger mMessenger = new Messenger(serviceIbinder); IPC example Thread safe Interprocess communication with an IBinder performed using a pool of threads in IBinder process ContentProvider methods called from a pool of threads in content provider's process query() insert() delete() update() LogCat Android logging system mechanism used to view system debug output Can be used to view stack trace of emulator errors Useful for locating line of code were error initiated LogCat viewable in realtime in Debug or DDMS view of Eclipse Common logging methods v - verbose d - debug i - information w - warning e - error Usage example Log.i("MyActivity", "MyClass.memberfunction – error message");
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Raf Simons
Slim-Fit Panelled Cotton Shirt
Unfortunately, this item will not be back in stock.
Raf Simons' latest collection featured this panelled shirt, a stylish option for summertime. The contrasting colours and short sleeves of this cotton piece combine to create a look that's offbeat but cool. Try it with tailored shorts and leather sandals for a sophisticated warm-weather outfit. Shown here with Wooyoungmi trousers, Luis Morais bracelet, Balenciaga bag and Valentino sneakers.
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Be Careem: 6 Ramadan initiatives the car hire app is supporting
How will you make a difference this Holy Month?July 2, 2015 3:00
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Abu Dhabi's TAQA drops 5.4 pct after saying no merger talks
Qatar's Doha Bank raises 2 bln riyal Tier 1 capital-boosting bond
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Dubai's port operator DP World shut down its Ain al-Sokhna port in Egypt for the second day on Monday because of labour strikes that have disrupted shipments, an official at the Red Sea port authority said.
February 14, 2012 2:44 by Reuters
Workers have been protesting for three days to press for hardship allowances, wage restructuring and a percentage of the firm's profits they say they are owed since 2008, he said.
Talks with the workers brokered by the Red Sea port authority failed after DP world said the workers had no right to demand any such commissions from the firm's profits, even though it was willing to offer hardship allowances.
"Our primary concern is the safety of our people. While we do not anticipate much disruption to our business, we hope to resolve the situation as soon as possible and swiftly resume normal operations," a DP World spokesman said.
Sokhna, near the southern end of the Suez Canal, is Cairo's main port for cargo from the Far East.
The company had also shut the port last September over a similar labour dispute.
DP World is considered one of the more profitable assets of debt-laden Dubai World. The port operator is the world's third largest and is 80-percent-owned by the state-linked conglomerate.
The firm temporarily suspended terminal operations at its Egyptian facility as a precautionary measure during the uprising that toppled President Hosni Mubarak earlier this year. (Reporting by Yusri Mohamed; Additional Reporting by Praveen Menon; Writing by Dina Zayed; Editing by Helen Massy-Beresford) *Image from alroyya.com
UAE's Aldar swings to profit on govt land sales
February 14 2012
UAE telco Etisalat wants piece of Libyan market
February 14 2012


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Oil Rub Brnz 3x60W Trestle Hanging Light
Company organisation and an international corporate structure is EGLO's key for the production and distribution of lights. Innovative market research, detailed orientation and a flexible approach lead the way to longlasting business partnerships with an independent family business.
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New Spring
By Robert Jordan; Mike Miller (Illustrator); Harvey Tolibao (Illustrator)
(Tor Books, Paperback, 9780765323804, 250pp.)
Publication Date: January 18, 2011
List Price: $24.99*
* Individual store prices may vary.
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In the last few years before his death, Robert Jordan worked closely with Chuck Dixon and Mike Miller on the graphic adaptation of "New Spring."
The eight full-color issues of "New Spring," released between 2005 and 2010, tell the story of the search for the infant Dragon Reborn and of the adventures of Moiraine Damodred, a young Aes Sedai, and Lan Mandragoran, the uncrowned king of a long-dead nation. Adapted by noted comics writer Chuck Dixon with the full cooperation of Robert Jordan, and illustrated by artists Mike Miller and Harvey Tolibao, "New Spring" will delight any of Robert Jordan's millions of readers.
Tor is proud to collect all eight issues of "New Spring "in a single volume, which will also include developmental art, script pages, and correspondence between Jordan and Dixon.
Mike is a veteran comic book artist, working on comic books ranging from Superman to Wolverine. Mike is also the cofounder and art director for Alias Enterprises and Cross Culture Entertainment.


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If you'd like to speak to us about teaching or teacher training, please contact our Teaching Line on Freephone
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Stock Vector Illustration:
Template menu and business card for cafe and restaurant
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Drink between: 2009 - 2011 (Add My Dates)
749 Tasting Notes
71 Tasting Notes
477 Tasting Notes
Really nice, great value. This combines the profile of tropical Chardonnay, waxy Chenin Blanc and the slightly oxidative-sherried style of López de Heredia white (Viura). Smooth, drinking at point right now, nothing profound, just an easy satisfying summer white.
Still great, but sliding -- drink up!
I'm glad I have another bottle of this as my last note did not do this justice- you have to dedicate time and attention to Abe's wines and I did not with my last bottle. The nose starts out with butterscotch and anise progressing to baked apple, a bit of caramel/toffee and even a hint of bacon. The palate is big but manages to keep the 15% abv fairly well hidden. Full-bodied with notes of butterscotch, herbs, and hazlenut. I personally prefer a bit more crispness, but a nice complexity makes up for it. Drinking well now or through 2013.
Once again, a total crowd-wower. I didn't need to sell it -- first sip found friends exclaiming and demanding explication. Butterscotch and an amazing refusal to self-classify along the mineral/buttery divide. The nose suggests you're diving into some stone, but upon arrival the malolactic elements are very pronounced. Fully rounded, butterscotch joy, with some grassy notes and some holiday spices in there to boot.
Verdhelo, delicious!


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The Reserve at Magnolia Ridge Apartments101 Harlon Dr., Cary, NC 27511
- $704 - $924
- 1 - 2 Beds
Note : Prices & Availability Subject To Change
The Reserve at Magnolia Ridge...
Experience Southern Charm in the Heart of Cary, North Carolina! The Reserve at Magnolia Ridge Apartments is easily accessible from I-40 and I-440 and is within walking distance of great shopping and dining options including Cary Town Center and Crossroads Shopping Center. Loaded with great floor plans, features, and amenities, you are sure to find your new home! Renovated units coming soon! Contact us for more details!
The Reserve at Magnolia Ridge is located in the Raleigh Area in the 27511 zip code. View this apartment on a map.
Why You'll Love Us
The Reserve at Magnolia Ridge is close to NC State University and many employers, including WakeMed Hospital. Our wooded community is close to I-40 and I-440. We offer great rates, on-call maintenance, tennis courts, a pool, and optional washer/dryer connections. Enjoy shopping and dining at Cary Crossroads or Cary Towne Center Mall, both within five miles.
Professionally Managed By: Greystone
Community Features
- 24-Hour Emergency Maintenance
- Clubhouse
- Laundry Facility
- Pet Friendly
- Swimming Pool
- Updated Fitness Center
- Additional Outside Storage
- Credit Cards Accepted
- Patios /Balconies
- Professional, On-Site Management Team
- Tennis Court (s)
- Vaulted Ceilings
Floor Plan Amenities
- Ceiling Fans
- Walk-in Closet (s)
- Fireplace
- Washer/Dryer Connections
*Note: In Select Units


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Tag Archives: Northern Illinois
CBD Around the Horn for Feb. 13th
Hey, it was finally opening day in College Baseball. As always, opening day is full of upsets from mid-major over teams with very high expectations. Here are some of the highlights of the first day of action. Continue reading CBD Around the Horn for Feb. 13th
2015 MAC Baseball Preview
Despite only losing four conference games and finishing with an overall record of 38-16, the Ball State Cardinals were left out of the postseason last year because they fell to the eventual MAC Conference Champion Kent State in the second round of the tournament. Continue reading 2015 MAC Baseball Preview
NIU releases 2015 Schedule
DeKALB – The NIU baseball team released its 2015 schedule Tuesday, a 56-game slate that will send the team to six different states, including Texas, where the Huskies will play 2014 College World Series participant Texas Tech.
Northern Illinois University and the Red Raiders will begin their four game series Friday, Feb. 20 and play two games on Saturday, Feb. 21. It will be NIU's seventh trip to Lubbock all-time and the first time the teams have played a full series since 2012, when NIU nearly secured an upset over the 24th ranked Red Raiders in the series finale, 3-2. Continue reading NIU releases 2015 Schedule
NIU's Ed Mathey Resigns
DeKALB, Ill. – Northern Illinois University Head Baseball Coach Ed Mathey has resigned his position with the school after 12 years at the helm of the Huskie program, NIU Associate Vice-President and Director of Athletics Sean T. Frazier announced Thursday. Continue reading NIU's Ed Mathey Resigns
CBD Photo Gallery: Oregon State 6 Northern Illinois 0
The Oregon State Beavers behind a 12 strikeout performance from Ben Wetzler picked up a 6-0 victory over the Northern Illinois Huskies. You can check out my photo gallery below from the game. Continue reading CBD Photo Gallery: Oregon State 6 Northern Illinois 0
2014 MAC Preseason Coaches Poll
CLEVELAND, Ohio – 2013 Regular Season Champion Kent State was picked to repeat as MAC East Division & Tournament Champions, the Mid-American Conference announced Wednesday. Ball State, last year's MAC Tournament runner-up was picked to win the West Division. The Flashes garnered four votes to win this year's MAC Tournament, while Ball State garnered three votes to win the Tournament crown and NCAA automatic bid. Bowling Green, Central Michigan, Eastern Michigan, Miami and Toledo each received one vote to win the Tournament. The preseason poll was voted on by the league's 12 Head Coaches. Continue reading 2014 MAC Preseason Coaches Poll
Northern Illinois releases 2014 Schedule
DeKALB, Ill. – Northern Illinois baseball, the defending Mid-American Conference West Division Champions, released its 2014 schedule Tuesday, featuring four teams with 2013 NCAA Tournament experience, including three teams who finished the season in multiple top-25 polls. Continue reading Northern Illinois releases 2014 Schedule
CBD Around The Horn (March 23)
Here are some of the top performances and highlights on the day. We will be providing daily articles as often as possible this season. If you know of an outstanding performance that we may have missed, feel free to send us an email by clicking here. Continue reading CBD Around The Horn (March 23)
CBD Around the Horn (March 2nd)
Here are some of the top performances and highlights on the day. We will be providing daily articles as often as possible this season. If you know of an outstanding performance we might miss, feel free to send us an email by clicking here.