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Chicken Salad Puffs
29 May 2009
Chicken Salad Puffs is a mix of salad and puff and goes well as an appetizer. These Chicken Salad Puffs are rich with the flavor of lemon, mayonnaise and butter. Give this yummy recipe a try.
|1⁄2 Cup (8 tbs)
|1⁄4 Cup (4 tbs)
|Sifted flour
|1⁄2 Cup (8 tbs)
|Cooked diced chicken
|2 Cup (32 tbs)
|Sliced celery
|1 Cup (16 tbs)
|3⁄4 Cup (12 tbs)
|1⁄3 Cup (5.33 tbs)
|Lemon juice
|1 Tablespoon
|Grated onion
|1 Teaspoon
|Worcestershire sauce
|1⁄4 Teaspoon
|Black pepper
|1⁄8 Teaspoon
|To Taste
Combine water and butter; heat to boiling.
Add flour; stir over direct heat until mixture forms a ball that follows spoon around the pan.
Remove from heat; add an egg at a time.
Beat vigorously until well blended after each addition.
Drop in four mounds onto ungreased baking sheet.
Bake in preheated 400 degree oven for 45 to 50 minutes.
Cool on wire rack.
Combine chicken, celery and olives.
Blend in all remaining ingredients; mix lightly with chicken mixture.
Split puffs; fill with chicken mixture.
NOTE: Flour mixture may be baked in tiny amounts for canapes.
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Chicken Salad Puffs Recipe


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Todd Hatton speaks with Dr. Gerry Mooney of The Open University in Scotland
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Dr. Gerry Mooney is on the Social Sciences faculty of The Open University in the United Kingdom. This week, he came to Murray State to speak about national identity, devolution, and Scottish independence. He also stopped by our studios to talk with Todd Hatton about how these three things will become more significant as democracy continues to develop around the world.


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A lovely yellow/green tint, nice nose with citrus, buttery, tropical notes. The palate is understated - some citrus, apple, pear, even mango - but all integrated into a lovely, mature, harmonious whole. Buttery, honeyed, supported by the subtle oak but elegant and 'un-shouty'. Beats a lot of cheaper Burgundy hands down for me.
I always enjoy Catena Chardonnay at all levels but like to keep it for a few years before drinking. This did not disappoint.
The perceptive comment about 'corn' by Whip Hand crystallised something for me but this component faded with a short time in the glass and with food (the wine is surely made for food) and did not detract from my enjoyment.
Always a favorite. Reasonably priced. Some oak, but not overly so. Buttery, with apples and pears.
Pear, and tropical fruits, with a hint of butter or vanilla, medium body, lingering finish
S: Vanilla, slight spicy hint, buttery creaminess, minerality, flint, peaches.
T: fairly good concentration, citrus, grape, vanilla, slightly buttery, apples.
All in all a decent wine with very good value.
Beautifully crafted rich, full bodied, ripe, creamy, partially oak-aged wine with plenty of freshness to give nice balance.
N: pear, jasmine, lemon
P: biting into a Granny Smith. Juicy and sweet at mid palate. Not long finish but mineral and tart finish. Nice balance 89. Ready to drink now.
Promising nose of restrained butter and minerals, but lighter on the palate. Finish was a little bitter, however.
Great Chardonney, very smooth and buttery
minerals, toast, pears, tropical fruit, buttery . one of my favourite chardonnays- restrained and burgundian, great value
Nothing special, but enjoyed at a birthday gathering with girl friends. A little on the sweet side and not very complex.
The nose is clean, medium in intensity, with predominantly lemon and lime aromas. The palate is dry, with pear and apple flavors. Very nicely balanced and with a light touch of oak, this is more Burgundian in style than Californian. Drinking well now but is likely to remain fresh and appealing for a couple of more years. A very good value at under $18.00, this paired really well with baked brie with pears.
Steady Chardonnay. Big Sweet fruit, butter and nice fresh acid. Good buy
possibly a bit unfair to score this wine as it was served a little too cold - this is a decent if slightly reserved Charoonnay
Nose was reluctant sweet honey. Sweet and buttery palate balanced with a crisp citrusy tropical fruit. Evolves to an anise based finish that has something like tar at the end. Big, disjointed, bossy and compelling. Perhaps would benefit from a year of incarceration. 13.5% alcohol. 87
Excellent chardonnay. Rich but not Heavy
very decent Argentinian Chardonnay - nice and dry but still fruity and buttery toasty
Medium straw core, moderate nose of lemon verbana, hay, buttered toast with vanilla and dill undertones. Full bodied flavors of lemon with a strong herbal presence of dill, hay and lemon verbana. The wine builds in body on the palate, finishing with wet stone, vanilla and hay. Fairly good.
This is the Chardonnay you've been looking for...darn tasty and perfect style for summer and 21st century style chard. Nose is light with some fruit, citrus, stewed fruit. Palate has nice acid, lemon/lime citrus that transforms into some nice hints of buttery/oak stewed fruit. Medium bodied, and still remains to be somewhat refreshing...great price point - 18$ CAN at Kenaston Wine. Would buy more for shelf.
Relativly cool Chardonnay. Sligtly smokey, some butter, vanilla and yellow fruit. Good acidity and freshness. Good value and fine alternative to commune level white burgundies.
Quite a charm, little subdued, some will call that in balance, I wanted for the moment some more extravaganza
Still on the younger side (I suspect it would age well over short period of time), but well balanced nevertheless.
Green apple, citrus and lemon custard pie flavors combined with toasty oak and great minerality make this wine pretty tasty.
Good value. Drink now or hold for a few years.
Enjoyed it with Poulet rôti (sounds better that rotisserie chicken from A&P? :=) )
A very nice wine, the 2009 Catena Chardonnay exhibits a nose full of fruit but also wet cold minerals and a crisp fresh acidity. The oak is there, but in a very subtle an pleasant way. The palate is medium-bodied, elegant and actually somewhat refined. Again the acidity plays an important role in the palate turning this effort away from the 'over-oaked' New World wines we are used to in the quality range. Overall impression is very positive.
Bodegas Catena Zapata (Mendoza): Well… not short on flavour but has a prominent yeasty tone that gets a bit overpowering in tandem with the full bodied fruit and the oak. Not sure I could have handled more than a glass.
Apple flavor with lemon finish. Full, but with little oak or buttery tones.


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After Ten Years: The Tyranny of Requirements
My good friend and colleague from the Flight Director's office, Jeff Bantle, left the agency several years ago to head up Lockheed-Martin's Presidential Helicopter project. He now has a great presentation talk about how incrementally added requirements can sink a … Continue reading
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Your green dots show that it's on somebody's honeydew list:
SFGov will get to this, someday…
Your green dots show that it's on somebody's honeydew list:
SFGov will get to this, someday…
This photo came from longtime SoMA resident Bluoz:
"New sign in the doorway of Marinello's School of Beauty in Mid Market, San Francisco, where all this takes place"
He continues:
"Help us have four more years of this. Vote for
Randy Shaw Jane Kim for District 6 Supervisor in San Francisco."
Here it is:
"A decade of data showing where people get arrested selling crack cocaine in SF"
The long street on the left side is Van Ness, the big diagonal is Market and the part shooting off down and to the right is 6th Street.
Anyway, this is what people mean when they refer to the Tenderloin:
I can't think of a thing that this map is missing.
What an excellent map!
Here's some graffiti placed on the Bay Bridge by people who didn't work hard enough to ensure that it was built correctly for a good price.
See it? It's says, "Mike Sucks Big Titties…" Hilarious:
So that's old news now, but here's some new bridge art, recorded by photographer Manny Chrisostomo of the Sacramento Bee:
See? It says "Chocolate Starfish."
Even more hilarious.
In other news, California High Speed Rail will never be "high speed" – it will be medium speed at best.
Oh well.
"When a friend of Sheryl Sandberg's was looking for a stock image of a female plumber, all she found online were women posing in lingerie and clutching wrenches."
Mmmm. What if I tried to find stock images of a "female plumber?" Here, take a look, no foolsies.
Well, wrenches, sure, that's the whole point. Holding a plumber's wrench = plumber, right?
But "posing in lingerie?" Not so much.
In fact not at all! But this one is close, I 'spose:
Gentle Reader, I'll make the impolite suggestion that billionaire* Sheryl Sandberg's conveniently-unidentified millionaire* friend doesn't know what she* is doing when she hunts for images online.
Why not sell books and lead by NOT (re)telling lies/stories that don't add up?
I guess the Epoch News does real news now? News to me. Anyway:
"Another thing Elizabeth Stampe would like to see is "road diets.' "They take a road that's kind of fat, like Sixth Street, which has a lot of lanes, and they reduce the number of travel lanes,' Stampe said."
This guy is still out there.
He is the Bringer of Earworms.
No pay but the perks just might be out of this world…
Click to expand
Hey, let's take measure of the "world-class" image of our "world-class" Mayor's "world-class" City:
"OH: "In no other place in America would you see so many crack pipes between Nordstoms and Bloomingdales." A DC-er's take on #sanfrancisco :)"
I'll tell you, I don't think I have ever seen the flames of a crackpipe IRL, but Bluoz has, certainly.
"Congratulations to Mayor Ed Lee for winning the mayor's race.
November 10, 2011, from the Ed Lee-Randy Shaw-Mid-Market-special-project-corrupt-Twitterloin-tax-area
4 more years of this – yay,
Yep, he 'get's it done' all right"
Marinello School of Beauty betwixt Sixth and Seventh:
Via Bluoz – click to expand


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China has declared that so-called "House Christians" are a cult and must be suppressed. The State Administration for Religious Affairs, which is the Communist watchdog agency in China responsible for supervising religious activity, claims 7 million members in its Three Self-Patriotic Movement (TSPM) and Pew Research estimates that there are 70 million Chinese Protestants who worship in their own home churches, ungoverned by the state. There is a similar split between state "catholicism," with about 5 million Chinese, and 12 million who follow traditional Catholic belief in the religious primacy of the Pope.
Despite Largescale Government Efforts to Deter Terrorists, Suicide Bombers Strike TwiceWritten by Muhammad Zamir Assadi
As many as 16 people lost their lives on December 8, while 31 others sustained injuries, in the first suicide attack during the commencement of the Islamic month Muharam-UL-haram in the Tirah Valley of the Kohat district of Pakistan, almost 150 kilometres away from the capital Islamabad.
As reported previously for The New American, a recent poll of public opinion in Germany revealed that only approximately one-third of Germans have a positive view of Muslims. In addition, only 30 percent of those living in western Germany and 20 percent of the residents in the eastern portion of nation favor permitting more mosques to be built.
British police arrested Julian Assange on December 7 over charges of sexual misconduct in Sweden. Accused by two women of rape, molestation, and sexual coercion, Assange agreed to give himself up to the British police but has vowed to contest extradition to Sweden. Some skeptics question, however, the validity of the charges levied against the founder of Wikileaks.


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The train is climbing the 4% grade to the top of Cumbres Pass. Lead engine #484 is around the bend in front with #487 & 488 following behind. This shot is from the cupola of short caboose #0579.... (more)
Mark Yates fills the lubricator on 487 prior to a rotary run up Cumbres.
A close up of 488 pushing at the rear of this freight train. Soni Honigger (in the cab) looks to be concentrating intently on his charge. The Flying Rio Grande on the tender looks great taking you... (more)
The temps are way below freezing during the night, steam engines tend to drip, ice forms, and the result by early morning is an engine solidly frozen to the rails. So the hostler burns some oil s... (more)
Cumbres and Toltec 484 and 487 begin to climb a grade.
Cumbres and Toltec 489 undergoing work in the Chama shops.
Engine 484 heads out of Chama with a full head of steam leading the 487 in the middle and 488 bringing up the rear. This train is part of Chama Steam VI.
Charging after me is 488 crossing the Rio Chama on a beautiful morning.
D&RGW 488 waits in front of the depot in Chama, New Mexico. In ten minutes it will be assulting Cumbres Pass.
Smoke towers out of 488's stack on its way up Cumbres Pass.
D&RGW 487 sits outside the shop in Chama, New Mexico. From this angle it looks pretty intimidating.
D&RGW 487 sits outside the Cumbres and Toltec shop in Chama, New Mexico.
Heading back to Chama, the train has just completed the horseshoe curve outside of Osier, some of which can be seen at the right.


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I'm kind of paralyzed right now. I have too many great books in my possession and they are all screaming at me to be read. Can't decide which! I'll have you do it for me!Read More
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And yes, you definitely need to read this one! A gorgeously-wrought tale filled with magic and wonder. I'm not the only one who thinks so.Read More
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Simultaneous determination of prochlorperazine and its metabolites in human plasma using isocratic liquid chromatography tandem mass spectrometry
ABSTRACT Oral prochlorperazine (PCZ), an antiemetic, undergoes extensive first-pass metabolism. The study developed a simultaneous analytical method for PCZ and its major metabolites, prochlorperazine sulfoxide (PCZSO), N-demethylprochlorperazine (NDPCZ) and 7-hydroxyprochlorperazine (PCZOH), in human plasma using an isocratic liquid chromatography-tandem mass spectrometry (LC-MS/MS) method. Deproteinized plasma specimens were separated using a 3 µm particle size octadecylsilyl column, and the run time was 10 min. The calibration curves were linear over the concentration ranges of 0.01-40 µg/L for PCZ, NDPCZ and PCZOH, and 0.05-80 µg/L for PCZSO. The intra- and inter-assay precisions and accuracies were within 7.0 and 99-104% and within 9.0 and 99-105%, respectively. The lower limits of quantification in human plasma were 10 ng/L for PCZ, NDPCZ and PCZOH, and 50 ng/L for PCZSO. The validated method was applied to the determination of plasma samples in 37 cancer patients receiving PCZ. Large interindividual variations were observed in plasma concentrations of PCZ, PCZSO, NDPCZ and PCZOH (relative standard deviation, 89.4, 88.7, 86.4 and 78.2%, respectively). In conclusion, this simultaneous LC-MS/MS method with acceptable analytical performance can be helpful for evaluating the pharmacokinetics of PCZ, including the determination of its metabolites in cancer patients and in clinical research.
Article: Impact of genetic and non-genetic factors on clinical responses to prochlorperazine in oxycodone-treated cancer patients[Show abstract] [Hide abstract]
ABSTRACT: The contributions of DRD2 and OPRM1 genetic variants to clinical responses to prochlorperazine remain to be clarified in opioid-treated patients. We evaluated the clinical responses to prochlorperazine based on non-genetic and genetic factors in oxycodone-treated patients. Seventy Japanese cancer patients starting oral prochlorperazine together with oxycodone were enrolled. Predose plasma prochlorperazine concentrations and serum prolactin concentrations were determined. The incidences of oxycodone-induced nausea and vomiting were monitored for 2weeks. Plasma prochlorperazine concentration and oxycodone daily dose were not associated with the incidences of nausea and vomiting. The incidence of nausea was significantly higher in the DRD2 TaqIA A1A2+A1A1 group than in the A2A2 group. The incidence of vomiting was significantly higher in females than in males. Before and after the prochlorperazine administration, the serum prolactin concentration was significantly higher in female patients than in male patients. The serum prolactin concentration was weakly correlated with prochlorperazine concentration and was significantly higher in the OPRM1 118AA group than in the AG+GG group. DRD2 TaqIA and female gender altered the prophylactic antiemetic efficacy of prochlorperazine. OPRM1 A118G together with plasma exposure of prochlorperazine and gender affected prolactin secretion in oxycodone-treated patients.Clinica chimica acta; international journal of clinical chemistry 12/2013; 429. DOI:10.1016/j.cca.2013.12.011 · 2.76 Impact Factor


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- blusky
- Reviews: 288
Hotel options in city center
If you're coming to Salerno by train and want to stay really near the city centre, you're lucky, becuase train station is situated in the city centre, actually on the main walking street of the city, from which you can start exploring it.
As for the possibilities of accomodation there are two options, actually two small hotels on the main street of Salerno, one is right accross the train station (Hotel Plaza) and the other one (Hotel Montestella) is max 5 minutes walk from the train station, on the main street.
Here are the links:
For those who prefer room with the view, as well as the possibility of hotel with small pool, sunbathing possibilities, good food and not too far away from city centre, there's Grand Hotel Salerno,not even 10 minutes walk from the train station.
- blusky
- Reviews: 288
Villa Poseidon: Panorma is great
I have been in their restaurant only, but the loby, the garden, the location is great and I'm sure that the rooms are as clean and excellent as the rest of the things I have seen there.
Location, food, relax...
They also organize wedding receptions.
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VIEWS: 4 PAGES: 18 CATEGORY: Organic Compounds POSTED ON: 8/6/2010 Public Domain
The present invention relates to compounds and methods for detecting biomolecules, and in particular, the detection of nucleic acid and nucleic acid hybridization using photocleavable phosphoramidites.BACKGROUNDNumerous methods have been developed for the incorporation of ligands into synthetic oligonucleotides using phosphoramidite chemistry. See S. L. Beaucage and P. I. Radhakrishnan, Tetrahedron 49:1925 (1993). One approach is based on theincorporation of an aliphatic amino group into synthetic oligonucleotides by use of an amino-modifier phosphoramidites or modified CPGs. See S. Agrawal et al., Nucleic Acids Res., 14: 6227 (1986). See P. S. Nelson et al., Nucleic Acids Res. 20:6253(1992); P. S. Nelson et al., Nucleic Acids Res. 17:7179 (1989); P. S. Nelson et al., Nucleic Acids Res. 17: 7187 (1989). Such amino groups allow the post-synthetic addition of a variety of molecules including fluorescent markers, biotin, haptens orenzymes which can be used as hybridization probes for non-radioactive detection of specific target DNA/RNA or as sequencing primers. Amino-modified oligonucleotides have also been used for preparation of affinity matrices and as immobilized PCR primers.One disadvantage of covalent labeling and immobilization of oligonucleotides is that the covalent bond is not selectively cleavable, and, therefore does not allow for the recovery/release of oligonucleotide or the removal of the label. In orderto circumvent this problem, several approaches have been proposed based on chemical cleavage. These include the use of cleavable disulfide bonds or the introduction of acid cleavable linkers. See W Bannwarth and J. Wippler, Helv. Chim. Acta 73:1139(1990); B. D. Gildea et al., Tetrahedron Lett. 31:7095 (1990); E.Leikauf et al., Tetrahedron 51:3793 (1995); J. Olejnik et al., Nucleic Acids Res. 24: 361 (1996). These approaches, however, require the application of chemical reagents that are notsuitable for many applications. They also necess
United States Patent: 6218530 ( 1 of 1 ) United States Patent 6,218,530 Rothschild , et al. April 17, 2001 Compounds and methods for detecting biomolecules Abstract Methods are described for synthesizing compounds useful for detecting nucleic acids, and in particular, the hybridization of nucleic acids. Photocleavable agents are described, including but not limited to photocleavable nucleotides and photocleavable phosphoramidites. Inventors: Rothschild; Kenneth J. (Newton, MA), Olejnik; Jerzy (Brookline, MA) Assignee: AmberGen Inc. (Boston, MA) Appl. No.: 09/323,424 Filed: June 1, 1999 Current U.S. Class: 536/25.32 ; 435/6; 536/22.1; 536/23.1; 536/24.3; 536/24.33; 536/25.3 Current International Class: C07H 21/00 (20060101); C12Q 001/68 (); C07H 019/00 (); C07H 021/00 (); C07H 021/02 (); C07H 021/04 () Field of Search: 435/6 536/22.1,23.1,26.2,24.33,25.3,25.32 References Cited [Referenced By] U.S. Patent Documents 5643722 July 1997 Rothschild et al. 5922858 July 1999 Rothschild et al. 5948624 September 1999 Rothschild et al. 5986076 November 1999 Rothschild et al. 6057096 May 2000 Rothschild et al. Other References Agrawal, S., Christodoulu, C., and Gait, M., "Efficient Methods For Attaching Non-Radioactive Label To The 5' Ends Of Synthetic Oligodeoxyribonucleotides," Nucleic Acids Research vol. 14 No. 15 pp. 6227-6245 (1986). . Bannwarth, W. and Wippler, J., "110. A new Combined Purification/Phosphorylation Procedure For Oligodeoxynucleotides," Helvetica Chimica Acta, vol. 73, pp. 1139-1147 (1990). . Beaucage, S. and Radhakrishnan, P., "The Functionalization Of Oligonucleotides Via Phosphoramidite Derivatives," Tetrahedron vol. 49, No. 10. pp. 1925-1963 (1993). . Gildea, B., Coull, J., and Koster, H., "A Versatile Acid-Labile Linker For Modification Of Synthetic Biomolecules," Tetrahedron Letters, vol. 31, No. 49, pp. 7095-7098 (1990). . Leikauf, E., Barnekow, F., and Koster, H., "Heterobifunctional Trityl Derivatives As Linking Reagents For The Recovery Of Nucleric Acids After Labeling And Immobilization," Tetrahedron vol. 51 No. 13, pp. 3793-3802 (1995). . Nelson, P., Frye R., and Edison, L., Bifunctional Oligonucleotide Probes Synthesized Using A Novel CPG Support Are Able To Dectect Single Base Pair Mutations Nucleic Acids Research, vol. 17 No. 18, pp. 7187-7195 (1989). . Nelson, P., Kent, M., and Muthini, S., "Oligonucleotide Labeling Methods 3. Direct Labeling Of Oligonucleotides Employing A Novel, Non-Nucleosidic, 2-Aminobutyl-1,3-Propanediol Backbone," Nucleic Acids Research vol. 20 No.23, pp. 6253-6259 (1992). . Nelson, P., Sherman-gold, R., and Leon, R., "A New And Versatile Reagent For Incorporating Mutiple Primary Aliphatic Amines Into Synthetic Oligonucleotides," Nucleic Acids Research vol. 17 No. 18, pp. 7179-7186 (1989). . Olejnik, J., Krymanska-Olejnik, E., and Rothschild, K., "Photocleavable Biotin Phosphoramidite For 5'-End-Labeling, Affinity Purification And Phosphorylation Of Synthetic Oligonucleotides," Nucleic Acids Research vol. 24 No. 2, pp. 361-366 (1996).. Primary Examiner: Riley; Jezia Attorney, Agent or Firm: Medlen & Carroll, LLP Government Interests This invention was made with government support under grants from the National Institutes of Health (GM54920-01) and the Army Research Office (ARO-DAAH04-96-C-0050). The government has certain rights in the invention. Parent Case Text This application for patent under 35 U.S.C. 111(a) claims priority to Provisional Applications Ser. Nos. 60/087,641 filed Jun. 2, 1998 under 35 U.S.C. 111(b). Claims What is claimed is: 1. A composition, comprising a photocleavable aminotag phosphoramidite selected from the group consisting of compounds 6 and 7 of FIG. 2. 2. The composition of claim 1, wherein said photocleavable aminotag phosphoramidite is incorporated at the 5' end of an oligonucleotide. 3. The composition of claim 2, wherein said oligonucleotide is immobilized to a surface via the 3'-end of said oligonucleotide. 4. The composition of claim 2, wherein said oligonucleotide is immobilized via said aminotag to an activated surface. 5. The composition of claim 4, wherein said activated surface comprises activated agarose. 6. The composition of claim 3, wherein said surface is the surface of a bead. 7. The composition of claim 2, wherein a marker molecule is linked to said aminotag phosphoramidite under conditions such that an Fmoc protecting group is removed from said aminotag phosmhoramidite. 8. The composition of claim 7, wherein said marker molecule is selected form the group consisting of activated polypeptides, activated biotin, activated digoxigenin, and activated tetramethylrhodamine. 9. The composition of claim 7, wherein said marker is detectable by spectrometry. 10. The composition of claim 9, wherein said marker is detectable by mass spectrometry. 11. The composition of claim 6, wherein said bead is a glass bead. 12. The composition of claim 11, wherein said glass bead is controlled pore glass. Description FIELD OF THE INVENTION The present invention relates to compounds and methods for detecting biomolecules, and in particular, the detection of nucleic acid and nucleic acid hybridization using photocleavable phosphoramidites. BACKGROUND Numerous methods have been developed for the incorporation of ligands into synthetic oligonucleotides using phosphoramidite chemistry. See S. L. Beaucage and P. I. Radhakrishnan, Tetrahedron 49:1925 (1993). One approach is based on the incorporation of an aliphatic amino group into synthetic oligonucleotides by use of an amino-modifier phosphoramidites or modified CPGs. See S. Agrawal et al., Nucleic Acids Res., 14: 6227 (1986). See P. S. Nelson et al., Nucleic Acids Res. 20:6253 (1992); P. S. Nelson et al., Nucleic Acids Res. 17:7179 (1989); P. S. Nelson et al., Nucleic Acids Res. 17: 7187 (1989). Such amino groups allow the post-synthetic addition of a variety of molecules including fluorescent markers, biotin, haptens or enzymes which can be used as hybridization probes for non-radioactive detection of specific target DNA/RNA or as sequencing primers. Amino-modified oligonucleotides have also been used for preparation of affinity matrices and as immobilized PCR primers. One disadvantage of covalent labeling and immobilization of oligonucleotides is that the covalent bond is not selectively cleavable, and, therefore does not allow for the recovery/release of oligonucleotide or the removal of the label. In order to circumvent this problem, several approaches have been proposed based on chemical cleavage. These include the use of cleavable disulfide bonds or the introduction of acid cleavable linkers. See W Bannwarth and J. Wippler, Helv. Chim. Acta 73:1139 (1990); B. D. Gildea et al., Tetrahedron Lett. 31:7095 (1990); E.Leikauf et al., Tetrahedron 51:3793 (1995); J. Olejnik et al., Nucleic Acids Res. 24: 361 (1996). These approaches, however, require the application of chemical reagents that are not suitable for many applications. They also necessitate removal of the excess of cleaving reagents and result in residual modification of the oligonucleotide. SUMMARY OF THE INVENTION The present invention relates to compounds and methods for detecting biomolecules, and in particular, the detection of nucleic acid and nucleic acid hybridization using photocleavable agents, including photocleavable detectable moieties, photocleavable nucleotides (e.g. photocleavable NTPs) and photocleavable phosphoramidites. It is not intended that the present invention be limited to the type of biomolecule detected. Both nucleic acids and proteins can be detected using the photocleavable agents of the present invention. The details regarding detection of various biomolecules (including proteins) are set forth in Attachments A, B, C, D and E hereto, all of which constitute the disclosure and specification of the present application. In one embodiment, the present invention contemplates a photocleavable nucleotide. In a specific embodiment, the photocleavable nucleotide is a photocleavable dideoxynucleotide. The latter is useful in a variety of formats and assays, including but not limited to, nucleic acid sequencing. In one embodiment, the present invention contemplates a composition, comprising a photocleavable aminotag phosphoramidite selected from the group consisting of compounds 6 and 7 of FIG. 2B. From FIG. 2B, it should be clear that the present invention contemplates particular protecting groups ("PG") suitable for such compounds, including but not limited to, 9-fluorenylmethoxycarbonyl (FMOC). In a preferred embodiment, the photocleavable aminotag phosphoramidite is incorporated into an oligonucleotide during synthesis at the 5' end of an oligonucleotide. In another embodiment, the present invention contemplates a composition, comprising a photocleavable sulfhydryltag phosphoramidite such as that shown in FIG. 4. In a preferred embodiment, the photocleavable sulfhydryltag phosphoramidite is incorporated at the 5' end of an oligonucleotide. The present invention also contemplates methods of synthesis of photocleavable phosphoramidites and methods of synthesis of oligonucleotides containing such photocleavable phosphoramidites. Moreover, the present invention contemplates methods of incorporating detectable moieties or markers ("M") to a phosphoramidite and photocleaving so as to release said marker. In a further embodiment, the present invention contemplates oligonucleotides linked to a solid support or surface via a photocleavable linkage. In such an embodiment, the oligonucleotide can be released from the solid support (e.g. a bead) or a surface (e.g. a gold surface) by photocleavage. DESCRIPTION OF THE DRAWINGS FIG. 1 schematically shows a generic structure for the photocleavable phosphoramidites of the present invention. FIGS. 2A and 2B schematically shows one embodiment of a synthesis scheme for a variety of aminotag phosphoramidites of the present invention. FIG. 3 schematically shows one embodiment of linking a marker ("M") to an aminotag phosphoramidite that has been incorporated into an oligonucleotide, followed by photocleavage. FIG. 4 shows the structure of one embodiment of the sulfhydryltag phosphoramidites of the present invention. FIG. 5 schematically shows one embodiment of a synthesis scheme for an oligonucleotide conjugate comprising a photocleavable aminotag phosphoramidite and a peptide marker (SEQ ID NO: 1). FIG. 6 schematically shows the detection of a peptide marker of an oligonucleotide conjugate by mass spectrometry. FIG. 7 shows one embodiment of a photocleavable nucleotide of the present invention. FIG. 8 shows two compounds of FIG. 7 where the protecting group is indicated generically as "PG". DEFINITIONS To facilitate understanding of the invention, a number of terms are defined below. The terms "marker" and "marker molecule" refer to a detectable moiety, i.e. a chemical group, structure of compound that possesses a specifically identifiable physical property which can be distinguished from the physical properties of other chemicals present in the heterologous mixture. It is not intended that the present invention be limited to a particular detectable moiety. For example, the present invention contemplates detectable moieties having the properties of fluorescence, phosphorescence and luminescence. A variety of molecules have such properties as set forth in U.S. application Ser. No. 08/345,807, hereby incorporated by reference. Preferred detectable moieties are those that are detectable by spectrometry, and in particular, by mass spectrometry. "Nucleic acid sequence" and "nucleotide sequence" as used herein refer to an oligonucleotide or polynucleotide, and fragments or portions thereof, and to DNA or RNA of genomic or synthetic origin which may be single- or double-stranded, and represent the sense or antisense strand. The photocleavable agents of the present invention can be "linked" or "incorporated" into proteins and nucleic acids. By use of the term "linked" or "incorporated" it is not meant that the entire photocleavable agent need be part of the final molecule. Some photocleavable agents of the present invention have reactive groups (i.e. the agent is an "activated agent") and leaving groups such that the photocleavable agent upon incorporation or linkage loses one or more groups. The term "portion" when used in reference to a nucleotide sequence refers to fragments of that nucleotide sequence. The fragments may range in size from 1 nucleotide residues (and more preferably 5 or greater) to the entire nucleotide sequence minus one nucleic acid residue. The present invention contemplates, for example, that the photocleavable agents may be incorporated into just a portion of a nucleic acid. As used herein, the terms "complementary" or "complementarity" are used in reference to "polynucleotides" and "oligonucleotides" (which are interchangeable terms that refer to a sequence of nucleotides) related by the base-pairing rules. For example, for the sequence 5'-AGT-3' is complementary to the sequence 5'-ACT-3'. Complementarity can be "partial" or "total." "Partial" complementarity is where one or more nucleic acid bases is not matched according to the base pairing rules. "Total" or "complete" complementarity between nucleic acids is where each and every nucleic acid base is matched with another base under the base pairing rules. The degree of complementarity between nucleic acid strands has significant effects on the efficiency and strength of hybridization between nucleic acid strands. The present invention contemplates hybridization (e.g. complete, partial, low and high stringency) using oligonucleotides having photocleavable agents incorporated therein. The terms "homology" and "homologous" as used herein in reference to nucleotide sequences refer to a degree of complementarity with other nucleotide sequences. There may be partial homology or complete homology (i.e., identity). A nucleotide sequence which is partially complementary, i.e., "substantially homologous," to a nucleic acid sequence is one that at least partially inhibits a completely complementary sequence from hybridizing to a target nucleic acid sequence. The inhibition of hybridization of the completely complementary sequence to the target sequence may be examined using a hybridization assay (Southern or Northern blot, solution hybridization and the like) under conditions of low stringency. A substantially homologous sequence or probe will compete for and inhibit the binding (i.e., the hybridization) of a completely homologous sequence to a target sequence under conditions of low stringency. This is not to say that conditions of low stringency are such that non-specific binding is permitted; low stringency conditions require that the binding of two sequences to one another be a specific (i.e., selective) interaction. The absence of non-specific binding may be tested by the use of a second target sequence which lacks even a partial degree of complementarity (e.g., less than about 30% identity); in the absence of non-specific binding the probe will not hybridize to the second non-complementary target. Low stringency conditions comprise conditions equivalent to binding or hybridization at 42.degree. C. in a solution consisting of 5.times. SSPE (43.8 g/l NaCl, 6.9 g/l NaH.sub.2 PO.sub.4.H.sub.2 O and 1.85 g/l EDTA, pH adjusted to 7.4 with NaOH), 0.1% SDS, 5.times. Denhardt's reagent [50.times. Denhardt's contains per 500 ml: 5 g Ficoll (Type 400, Pharmacia), 5 g BSA (Fraction V; Sigma)] and 100 .mu.g/ml denatured salmon sperm DNA followed by washing in a solution comprising 5.times. SSPE, 0.1% SDS at 42.degree. C. when a probe of about 500 nucleotides in length is employed. The art knows well that numerous equivalent conditions may be employed to comprise low stringency conditions; factors such as the length and nature (DNA, RNA, base composition) of the probe and nature of the target (DNA, RNA, base composition, present in solution or immobilized, etc.) and the concentration of the salts and other components (e.g., the presence or absence of formamide, dextran sulfate, polyethylene glycol), as well as components of the hybridization solution may be varied to generate conditions of low stringency hybridization different from, but equivalent to, the above listed conditions. In addition, the art knows conditions which promote hybridization under conditions of high stringency (e.g., increasing the temperature of the hybridization and/or wash steps, the use of formamide in the hybridization solution, etc.). When used in reference to a double-stranded nucleic acid sequence such as a cDNA or genomic clone, the term "substantially homologous" refers to any probe which can hybridize to either or both strands of the double-stranded nucleic acid sequence under conditions of low stringency as described above. When used in reference to a single-stranded nucleic acid sequence, the term "substantially homologous" refers to any probe which can hybridize (i.e., it is the complement of) to the single-stranded nucleic acid sequence under conditions of low stringency as described above. As used herein, the term "hybridization" is used in reference to the pairing of complementary nucleic acids using any process by which a strand of nucleic acid joins with a complementary strand through base pairing to form a hybridization complex. Hybridization and the strength of hybridization (i.e., the strength of the association between the nucleic acids) is impacted by such factors as the degree of complementarity between the nucleic acids, stringency of the conditions involved, the T.sub.m of the formed hybrid, and the G:C ratio within the nucleic acids. As used herein the term "hybridization complex" refers to a complex formed between two nucleic acid sequences by virtue of the formation of hydrogen bounds between complementary G and C bases and between complementary A and T bases; these hydrogen bonds may be further stabilized by base stacking interactions. The two complementary nucleic acid sequences hydrogen bond in an antiparallel configuration. A hybridization complex may be formed in solution (as determined, e.g., by C.sub.0.sup.t or R.sub.0.sup.t analysis) or between one nucleic acid sequence present in solution and another nucleic acid sequence immobilized to a solid support [e.g., a nylon membrane or a nitrocellulose filter as employed in Southern and Northern blotting, dot blotting or a glass slide as employed in in situ hybridization, including FISH (fluorescent in situ hybridization)]. "Stringency" when used in reference to nucleic acid hybridization typically occurs in a range from about T.sub.m -5.degree. C. (5.degree. C. below the T.sub.m of the probe) to about 20.degree. C. to 25.degree. C. below T.sub.m. The term "T.sub.m " is used in reference to the "melting temperature." The melting temperature is the temperature at which a population of double-stranded nucleic acid molecules becomes half dissociated into single strands. As will be understood by those of skill in the art, a stringent hybridization can be used to identify or detect identical polynucleotide sequences or to identify or detect similar or related polynucleotide sequences. Under "stringent conditions" a sequence or fragments thereof will hybridize to the sequence's exact complement and closely related sequences. DNA molecules are said to have "5' ends" and "3' ends" because mononucleotides are reacted to make oligonucleotides in a manner such that the 5' phosphate of one mononucleotide pentose ring is attached to the 3' oxygen of its neighbor in one direction via a phosphodiester linkage. Therefore, an end of an oligonucleotide is referred to as the "5' end" if its 5' phosphate is not linked to the 3' oxygen of a mononucleotide pentose ring. An end of an oligonucleotide is referred to as the "3' end" if its 3' oxygen is not linked to a 5' phosphate of another mononucleotide pentose ring. As used herein, a nucleic acid sequence, even if internal to a larger oligonucleotide, also may be said to have 5' and 3' ends. In either a linear or circular DNA molecule, discrete elements are referred to as being "upstream" or 5' of the "downstream" or 3' elements. This terminology reflects the fact that transcription proceeds in a 5' to 3' fashion. DESCRIPTION OF THE INVENTION The present invention relates to compounds and methods for detecting biomolecules, and in particular, the detection of nucleic acid and nucleic acid hybridization using photocleavable agents, including photocleavable detectable moieties, photocleavable nucleotides and photocleavable phosphoramidites. FIG. 1 schematically shows a generic structure for the photocleavable phosphoramidites of the present invention In one embodiment, the present invention contemplates non-nucleosidic photocleavable phosphoramidites. These reagents introduce a photocleavable amino group on the 5'-terminal phophate of synthetic oligonucleotides and are fully compatible with automated solid support synthesis. The 5' photocleavable amino group enables introduction of a variety of amine-reactive markers onto synthetic oligonucleotides as well as immobilization on activated solid supports. The photocleavable ("PC") bond on the 5'-phosphate can then be selectively cleaved by near-UV illumination thereby enabling release of the marker or detachment of the oligonucleotide from a solid support. It is not intended that the present invention be limited to the particular marker linked to the 5' terminal phosphate of the oligonucleotide. The preparation of photocleavable conjugates with polypeptides, biotin, digoxigenin, and tetramethylrhodamine are contemplated. It is specifically contemplated that the 5'-PC-amino group can be used as an affinity tag for photocleavage-mediated affinity purification and phosphorylation of synthetic oligonucleotides in conjunction with activated supports. Upon irradiation with 300-350 nm light, the 5'-PC-amino group is cleaved with high efficiency, resulting in rapid release of 5'-phosphorylated oligonucleotides into solution. Such 5'-PC-arnino labeled oligonucleotides are useful in a variety of applications in molecular biology including multiple non-radioactive probing of DNA/RNA blots, affinity isolation and purification of nucleic acids binding proteins, diagnostic assays requiring release of the probe-target complex or specific marker, cassette mutagenesis and PCR. They enable the spatially-addressable photorelease of the probe-target complexes or marker molecules for diagnostic purposes. A. Photocleavable AminoTag Phosphoramidites A variety of aminotag phosphoramidites are contemplated. In one embodiment (FIG. 2B), the PC-aminotag phosphoramidite (compound 6) comprises a protected amino group linked through a methylene group to a photocleavable 1-(2-nitrophenyl)ethyl linker derivatized with N,N'-diisopropyl-2-cyanoethyl phosphoramidite. In another embodiment, the present invention contemplates a "PC-X-aminotag" (FIG. 2B, compound 7) which comprises an additional spacer (e.g. 6-aminocaproic acid, referred to as "X") between the amino group and the photocleavable linker. The properties of modified oligonucleotides prepared using these PC-aminotag phosphoramidites and their conjugates are such that the present invention provides advantages over other cleavable systems (discussed above). Unlike biotin, which can interact only with avidin/streptavidin coated surfaces, PC-aminotag-phosphoramidite introduces a photocleavable amino group onto the 5' end of an oligonucleotide. As such it can be used to react with a wide variety of activated surfacs and molecules, thereby facilitating the formation of many different PC-linkers and PC-markers. B. Synthesis of Oligonucleotides With AminoTag Phosphoramidites The photocleavable-aminotag phosphoramidites of the present invention can be readily used in any automated DNA/RNA synthesizer employing standard phosphoramidite chemistry (i.e. an oligonucleotide synthesis means). The selective reaction of PC-aminotag phosphoramidites with the free 5'-OH group of a growing oligonucleotide chain, followed by cleavage from the support and deprotection, results in the introduction of a phosphodiester group linked to a primary aliphatic amino group through a photocleavable linker. This amino group can then be used to introduce a variety of photocleavable markers through a postsynthetic modification reaction (FIG. 3) with amine reactive reagents. It can also be used to attach synthetic oligonucleotides to activated solid supports for a variety of applications requiring immobilization and release of DNA molecules. Upon irradiation with near-UV light, the phosphodiester bond between the photocleavable linker and the phosphate is cleaved, resulting in the formation of a 5'-monophosphate on the released oligonucleotide. The 1-(2-nitrophenyl)ethyl moiety is converted into a 2-nitrosoacetophenone derivative and is released together with the attached marker molecule M. C. Photocleavable SulfhydrylTag Phosphoramidites The present invention also contemplates sulfhydryltag phosphoramidites. The design of such compounds (FIG. 4) is similar to that for the aminotag phosphoramidites such that the photocleavable sulfhydryl group can be incorporated at the 5'-end of an oligonucleotide. This phosphoramidite allows for reactions with a variety of thiol-activated supports and biomolecules. A trityl protective group is also incorporated for protection purposes. D. Photocleavable Phosphoramidites Comprising Peptide Markers The present invention contemplates photocleavable phosphoramidites comprising peptide markers. Peptides as markers have a number of desirable features when used as mass markers, including a large set of distinct masses which are easily resolved by MALDI-MS. When attached through a photocleavable linker to a specific oligonucleotide sequence, the resulting conjugate can be used in a wide variety of applications in DNA-analysis by mass spectrometry. The compounds can be made using a conventional solid phase nucleic acid synthesizer (e.g. a synthesis means). The DNA probe is first asembled on an automated DNA synthesizer in the conventional fashion (FIG. 5). The amintotag phosphoramidite is incorporated at the 5' end of the sequence. At the end of the cycle, the support (CPG) is treated with base, which selectively removes the Fmoc protective group from the linker. In the next step, an N-Fmoc protected peptide marker is introduced and an activating mixture is added, resulting in the formation of the peptide bond between the peptide marker and photocleavable linker DNA. Conventional cleavage and deprotection with ammonia yields the desired conjugate with a free amino group on the peptide marker. E. Detection Of Peptide Markers The present invention contemplates the detection of markers, including but not limited to peptide markers. In one embodiment, the present invention contemplates detection by mass spectrometry (e.g. MALDI-MS). In one embodiment, the DNA moeity of the mass marker is hybridized to an immobilized target DNA single strand. The bond between the hybridization probe and the mass tag is then cleaved by the UV-MALDI desorption laser pulse (FIG. 6) and the released mass tag (e.g. an octapeptide mass tag) is detected in the mass spectrum indicating the presence of the complementary sequence of its hybridization probe. This method potentially offers both higher sensitivity compared to the direct detection of the DNA hybridization probe and is less prone to signal degradation due to metastable fragmentation and cation adduct formation. Furthermore, it allows for simultaneous detection of several target sequences, since peptide mass markers can be readily synthesized with easily resolvable mass differences. F. Photocleavable Nucleotides The present invention also contemplates photocleavable nucleotides (PC-nucleotides). Using these PC-nucleotides, photocleavable tags (PC-tags) can be enzymatically incorporated into an oligonucleotide through a wide range of enzyme based methodologies for the synthesis and modification of DNA/RNA. These include PCR, terminal deoxynucleotidyl transferase (single labeling or tailing at the 3'-end) and nick translation reaction or replacement synthesis using T4 DNA polymerase (multiple label incorporation). For RNA labeling, appropriate polymerases, such as SP6, T7 and T3 polymerases can be used. Alternatively, T4 RNA ligase can be used to join 3'-labeled (deoxy)nucleotide 5'-monophosphate to target RNA. Dideoxy-PC-nucleotides can be used to end-label the products of polymerase based sequencing reactions thereby facilitating MALDI-MS based sequencing. Experimental The following examples serve to illustrate certain preferred embodiments and aspects of the present invention and are not to be construed as limiting the scope thereof. In the experimental disclosure which follows, the following abbreviations apply: eq (equivalents); .mu. (micron); M (Molar); .mu.M (micromolar); mM (millimolar); N (Normal); mol (moles); mmol (millimoles); .mu.mol (micromoles); nmol (nanomoles); g (grams); mg (milligrams); .mu.g (micrograms); ng (nanograms); L (liters); ml (milliliters); .mu.l (microliters); cm (centimeters); mm (millimeters); .mu.m (micrometers); nM (nanomolar);.degree. C. (degrees Centigrade); PBS (phosphate buffered saline); TLC (thin layer chromatography); HPTLC (high performance thin layer chromatography); HPLC (high pressure liquid chromatography); U (units); d(days). All chemicals used in the synthesis were purchased from Aldrich Chemical Co. (Milwaukee, Wis.), unless otherwise indicated. Biotinamidohexanoic acid NHS ester was purchased from Sigma (St. Louis, Mo.), digoxigenin-X-NHS ester (digoxigenin-3-O-methylcarbonyl-(-aminocaproic acid N-hydroxysuccinimide ester) was obtained from Boehringer-Mannheim (Indianapolis, Ind.) and tetramethylrhodamine-X-NHS ester (6-tetramethylrhodamine-5-(and-6)-carboxamidohexanoic acid succinimidyl ester, TAMRA-X-NHS) was from Molecular Probes (Eugene, Oreg.). Fmoc-NHS and N-Fmoc-6-aminocaproic acid were from Novabiochem (La Jolla, Calif.). 1H NMR spectra were recorded in CDCl3 on a Varian Unity Plus (Palo Alto, Calif.) spectrometer at 400 MHz with chemical shifts ((, ppm) reported relative to a tetramethylsilane internal standard. Oligonucleotide synthesis was performed on an Applied Biosystem DNA/RNA synthesizer, model 392 (Foster City, Calif.). Samples were irradiated with a Blak Ray XX-15 UV lamp (Ultraviolet Products Inc., San Gabriel, Calif.) at a distance of 15 cm (emission peak 365 nm; 300 nm cut-off; intensity 2.3 mW/cm2@365 nm). UV-VIS spectra were recorded on a Shimadzu 2101PC spectrophotometer (Columbia, Md.). HPLC analysis was performed on a Waters (Milford, Mass.) system consisting of a U6K injector, 600 Controller, Novapak C18 (3.9.times.150 mm) column and a 996 photodiode array detector. Buffer A: 0.1 N triethylamine acetate, pH=7.0; buffer B: acetonitrile. Elution was performed using a linear gradient (8-50%) of buffer B in buffer A over 45 minutes at a 1 ml/min. flow. Preparative purifications were achieved on a Waters Novapak C18 RCM cartridge (8.times.100 mm) using conditions as specified above, except for flow rate, which was increased to 2 ml/min. Fractions were then analyzed by analytical HPLC, fractions pooled and freeze-dried. No special precautions were necessary to protect the reagents and the labeled oligonucleotides from light. EXAMPLE 1 This example describes the synthesis of 5-N-Fmoc(aminomethyl)-1-(2-nitrophenyl)-ethanol (FIG. 2B, compound 4). As shown in FIG. 2A, this compound can be obtained from 5-bromomethyl-2-nitroacetophenone (compound 1) through a quarternization reaction with hexamethylenetetramine and decomposition of the resulting quaternary salt (compound 2) under acidic conditions. Crude 5-aminomethyl-2-nitroacetophenone hydrochloride (2.5 mmole) was resuspended in 12 ml of methanol and cooled in an ice bath. 48 mg (1.26 mmole, 2.0 eq.) of sodium borohydride was added in small portions with continous stirring during 10 minutes. Subsequent portions of borohydride (30 mg each) were added to reaction mixture until TLC showed complete conversion (FIG. 2, compound 3). The reaction was quenched by addition of 10 ml acetone. 10 ml water was added and the reaction mixture was concentrated under vacuum to approximately 10 mL. 10 ml of acetonitrile was added and the pH of the resulting solution was adjusted to 8.5 using 1N HCl. Fmoc-NHS (1.03 g; 3 mmole, 1.2 eq. in acetonitrile (10 ml) was added slowly during 15 minutes, the pH being adjusted to 8.5 with triethylamine. The reaction mixture was stirred for an additional 1 hour, then it was acidified to pH=3 and extracted with chloroform. The organic layer was dried over anhydrous sodium sulphate and evaporated, yielding 1.4 g of compound 4 as yellow oil, which was purified on a silica gel column using a step gradient (0-2%) of methanol in chloroform. The product was isolated at 1% of MeOH. Yield -720 mg (69%) 1H NMR (CDCl3+CD3OD): 1.57-1.52 (d, 3H), 2.39 (s, 1H), 4.17-4.19 (m, 1H), 4.39-4.47 (d-d, 2H), 5.34-5.36 (q, 1H), 5.83 (s-br, 1H), 7.17-7.19 (d, 1H), 7.21-7.30 (t, 2H), 7.36-7.40 (t, 2H), 7.55-7.57 (d, 2H), 7.69 (s, 1H), 7.73-7.75 (d, 2H), 7.83-7.85 (d, 1H). EXAMPLE 2 This example describes the synthesis of compound 5 (FIG. 2B). This reaction was carried out as for compound 4 (See Example 1), except N-Fmoc-6-aminocaproic acid NHS ester was used instead of the Fmoc-NHS. To the solution of compound 3 (5.3 mmol in 40 ml acetonitrile:H2O, 1:1, pH=8.5) a solution of N-Fmoc-6-aminohexanoic acid NHS ester (2.9 g; 1.2 eq.) in 20 ml of dioxane was added during 15 minutes. After work-up, the crude product was purified on a silica gel column using step gradient of methanol in chloroform (0-3%), product (compound 5) being eluted at 2% methanol. Yield -2.03 g (72%). Rf=(CHCl3: MeOH, 95:5, v:v) 1H NMR (CDCl3+CD30D): 7.79-7.77(d, 1H), 7.72-7.67(m, 3H), 7.60-7.50(d, 2H), 7.35-7.31(t, 2H), 7.27-7.19(m, 3H), 6.42-6.37(app. t, br, 1H), 5.37-5.33(q, 1H), 5.16-5.08(t, br, 1H), 4.46-4.41(m, 2H), 4.33-4.30(d, 2H), 4.15-4.10(t, 1H), 3.14-3.09(q, 2H), 2.23-2.18(t, 2H), 1.67-1.59(m, 2H), 1.48-1.42(m, 3H), 1.35-1.28(m, 2H). EXAMPLE 3 This example describes the synthesis of yet another photocleavable aminotag phosphoramidite of the present invention, namely 5-N-Fmoc-(aminomethyl)-1-(2-nitrophenyl)ethyl-2-cyanoethyl-N,N-diisopropyl amino-phosphoramidite (compound 6, FIG. 2B). For this reaction, compound 4 (200 mg, 0.52 mmole) was dried in vacuo over P.sub.2 O.sub.5 for 5 hrs. 3 ml of anhydrous acetonitrile was injected through septum, followed by N,N-diisopropylethylamine (350 .mu.l, 2.08 mmole, 4 eq.) and finally cyanoethoxy-N,N-diisopropylchlorophosphine (0.52 mmole, 117 .mu.l, 1 eq.). The reaction mixture was stirred at RT for 1 hr then another 0.5 eq. of phosphine was added. After 2 hours the reaction mixture was treated with 1 ml of ethyl acetate and worked-up between aq. NaCl and dichloromethane. The organic layer was washed with water, dried over sodium sulphate and evaporated. Product was purified on silica gel column using a step gradient of ethyl acetate in hexane (0-30%) containing 0.2% of triethylamine. Product was isolated at 22% of ethyl acetate, yielding 130 mg of compound 6 (42%). Rf (hexane:ethyl acetate:Et3N, 45:45:10, v:v:v) 0.80 and 0.86 (two diastereoisomers). ESI-MS M+=619.3 (+H) (618.26 calculated). Elemental analysis: CHN %(calculated); C64.03(64.07); H6.23(6.35); N8.76(9.06). 1H NMR (CDCl3): 7.88-7.84(m, 1H), 7.77-7.27(m, 3H), 7.60-7.55 (m, 2H), 7.40-7.34 (m, 2H), 7.30-7.24(m, 3H), 5.67(t, 1H), 5.55-5.49(q, 1H), 4.45-4.37(m, 4H), 4.24-4.16(m,1H), 3.87-3.79(m, 1H), 3.65-3.58(m, 2H), 3.48-3.42(m, 1H), 2.62-2.40(m, 2H), 1.55-1.53(d, J=6.8 Hz+s, 4H), 1.19-1.08(d+d+d, J=6.8 Hz, 12H), 0.89-0.85(d, J=6.8 Hz, 3H). EXAMPLE 4 This example describes the synthesis of an oligonucleotide comprising a photocleavable aminotag phosphoramidite, namely Compound 7 (FIG. 2B). This compound was prepared from compound 5 (1.0 g; 1.92 mmol) N,N-diisopropylethylamine (1.3 ml; 4 eq.) and cyanoethoxy-N,N-diisopropylchlorophosphine (1.5 eq.) in 12 ml acetonitrile using a procedure similar to that described for compound 6. Product was purified on a silica gel column using step gradient of ethyl acetate in hexane (10-70%, v/v) containing 0.8% triethylamine. Product was isolated at 50% ethyl acetate as two diastereoisomers (Rf=0.69, 0.61; AcOEt : hexane : Et3N, 8:1:1, v/v). Yield -580 mg (41%). ESI-MS, M+=731.2 (731.3, calculated); elemental analysis C 65.34(64.01) H 6.65(6.89) N 10.08(9.57) (calculated) 1H NMR: 7.84-7.82(d, J=8.4, 1H), 7.72-7.69 (m, 3H), 7.57-7.52 (m, 2H), 7.37-7.32(m, 2H), 7.29-7.25(m, 3H), 6.73-6.65(t, J=6 Hz, 1H), 5.49-5.44(q, 1H), 5.10-5.00(t, 1H), 4.48-4.30(m, 4H), 4.20-4.05(m, 2H), 3.50-3.45(m, 2H), 2.66-2.64(m, 2H), 2.22-2.16(t, 2H), 1.54-1.48(d, J=6.0 Hz, 4H), 1.24-1.20(m, 6H), 1.16-1.13(d, J=6.4 Hz, 4H), 1.09-1.05(d, J=6.8 Hz, 4H) EXAMPLE 5 This example describes the syntheses of 5'-PC-amino-olignucleotides. The reaction was carried out using a commercially available synthesizer in the Trityl-On mode. Coupling efficiency for PC-aminotag phosphoramidites was determined by HPLC analysis of several sequences and found to be in the range 95-98%. Standard cleavage and deprotection procedures were used. The composition of 5'-PC-amino-(dT).sub.7 and 5'-PC-amino-X-(dT).sub.7 was verified before and after photocleavage reaction by MALDI-TOF: 5'-PC-amino-(dT).sub.7 -2326.3 (uncleaved) 2,148.4 (cleaved); 5'-PC-amino-X-(dT).sub.7 2,438.4 (uncleaved) 2,147.2 (cleaved). Control 5'-phosphorylated sequences were synthesized using the chemical phosphorylation reagent Phosphalink" (Applied Biosystem, Foster City, Calif.) according to the manufacturer's instructions. EXAMPLE 6 This example describes the reaction of the oligonucleotides of Example 5 with marker molecules. Crude 5'-PC-amino-d(T).sub.7 or 5'-PC-amino-X-d(T).sub.7 (5.0 OD) was dissolved in 200 .mu.l of 0.1 N NaHCO.sub.3 (pH=8.5). To this solution was added 100 .mu.l of the NHS ester (biotin, digoxigenin or rhodamine) solution in anhydrous DMF (10 mg/ml). The solution was incubated overnight at room temperature, then it was purified by gel filtration (Sephadex G-25, NAP-10 column, Pharmacia, Piscataway, N.J.), freeze dried and purified to homogeneity on RP HPLC. For photocleavage studies, a solution of 0.2 OD of oligonucleotide in 50 .mu.l of H.sub.2 O was irradiated for 5 minutes in clear polypropylene microcentrifuge tube and a 20 .mu.l aliquot was then analyzed by HPLC. EXAMPLE 7 This example describes reactions with activated supports. Two oligonucleotides, 44-mer(5'-H2N-PC-GGC-CGC-TAC-CTC-TCA-(CCA)6-CTG-ATG-AGC-T-3' SEQ ID NO: 2) and 35-mer (5'-H2N-PC-CAT-CAG-(TGG)6-TGA-GAG-GTA-GC-3' SEQ ID NO: 3) were synthesized at 0.2 .mu.mol scale with 5'-PC-aminotag modifier. These were cleaved and deprotected using standard procedures (28% NH3, 1 hr RT, 18 hrs 55.degree. C.) and then freeze-dried. 6-aminohexanoic acid-NHS agarose (100 mg) (Sigma, St. Louis, Mo.) was suspended in 300 .mu.l of water, vortexed briefly and spin-filtered using a 0.22 .mu.m Ultrafree MC filter (Millipore, Bedford, Mass.), then resuspended in 300 .mu.l of water. Crude 5'-PC-amino-oligonucleotide (10.0 OD) was added to a suspension of activated agarose, followed by 40 .mu.l of 1M NaHCO3. The mixture was vortexed at room temperature for 1 hour, spin-filtered and washed with 100 .mu.l of wash buffer (2M NaCl, EDTA, pH=8.0). Finally, the beads were resuspended in 300 .mu.l of wash buffer and irradiated with near UV light for 5 minutes. After irradiation, the suspension was spin-filtered and the beads were washed with wash buffer (100 .mu.l). The combined filtrates were then desalted on a NAP-10 column, analyzed by UV absorption spectroscopy and by denaturing PAGE. EXAMPLE 8 This example describes the detection of a peptide mass marker. All DNA syntheses were performed on an Expedite 8909 synthesizer (PerSeptive Biosystems, Framingham, Mass.) using phosphoramidite chemistry at either 0.2 or 1.0 .mu.mol scale. For the preparation of photocleavable peptide-DNA mass markers, DNA was assembled in the synthesizer at 1.0 .mu.mol scale and modified on the 5.mu.-end with PC-Aminotag phosphoramidite. The Fmoc protective group was then removed by brief treatment with tetramethylguanidine, the support was washed with acetonitrile, dried and coupled with activated, Fmoc protected peptide for 2 h. The support was washed with DMF and acetonitrile, dried and then deprotected with ammonia and freeze-dried. The conjugate was purified to homogeneity using RP-HPLC. For this presentation 3 different mass markers were synthesized (see Table 1). TABLE 1 Mass Tag Intact Mass # Composition Of Mass Marker (M + H).sup.+ Marker (M + H).sup.+ 1 H.sub.2 N-GRALGVFV-PC-5'-CAC-GTA-CAG-GAT-GTA-CAG-3' SEQ ID NO: 4 1095.4 Da 6707.0 Da 2 H.sub.2 N-GRALGVFL-PC-5'-TCG-AGA-GGT-ACA-TCG-TG-3' SEQ ID NO: 5 1109.4 Da 6438.8 Da 3 H.sub.2 N-GRALGVFF-PC-5'-AAG-CGG-TAC-GAG-TAG-CA-3' SEQ ID NO: 6 1143.4 Da 6490.9 Da Target DNA was synthesized on DMT-C27 beads (Penninsula Laboratories, Calif.). An aliquot containing approximately 4 nmol of target DNA was taken from the bead suspension and mixed with 10 pmol of the PC mass markers. Hybridization was carried out in hybridization buffer (25 mM NaCl, 20 mM Tris-HCl, 10 mM MgCl.sub.2, pH=7.5) under the following conditions: denaturation: 72.degree. C., 10 min., annealing: 72-37.degree. C. over 30 min. Samples were incubated for an additional 30 min. at 37.degree. C., centrifuged, washed with ice-cold water (3.times.100 .mu.l) and resuspended in 50 .mu.l water. MALDI-MS measurements on immobilized DNA target sequences were carried out on a Vision 2000 reflectron-TOF mass spectrometer: (U.sub.acc =5 kV, eq. flight path length 1.7 m, N.sub.2 laser, .lambda.=337 nm, .tau.=3 ns, SEM detector). Solution spectra were obtained on a laboratory built linear TOF-MS instrument equipped with delayed ion extraction (U.sub.acc =25 kV, equivalent flight path length 12.1 m, Nd:YAG laser, .lambda.=355 nm, .tau.=5 ns, additional Er:YAG laser, .lambda.=2.94 pm, .tau.=80 ns, MCP detector) 3-hydroxypicolinic acid and 2,5-dihydroxybenzoic acid were used as matrices for UV- MALDI measurements; for IR-MALDI measurements, succinic acid was used. For measurements on immobilized DNA, a 0.5-1 .mu.I aliquot of the bead suspension was codeposited on the target with 1-2 .mu.l of matrix solution (dried droplet preparation). In UV-MALDI mass spectra obtained from solutions of each of the mass markers, signals corresponding to the released peptide mass tag, the cleaved DNA and the intact conjugate were observed. In control experiments with IR-MALDI, only a signal corresponding to the intact mass marker and a very weak signal corresponding to the cleaved DNA sequence are detected, while signals of the peptide mass tag are absent. This proves the postulated photocleavage mechanism. Signals corresponding to mass tags released from mass markers hybridized to immobilized DNA target sequences were readily obtained in Uv-MALDI. In a competition experiment, an equimolar mixture of the 3 mass markers was incubated with beads presenting a DNA sequence complementary to the hybridization probe regions of one of them. Only the mass tag corresponding to the target sequence was detected in UV-MALDI mass spectra obtained from these beads, demonstrating the selectivity of hybridization. In a different experiment evaluating the possibility of multiplex detection of several target sequences an immobilized strand containing the three target sequences with two nucleotides in between was incubated with an equimolar solution of the 3 mass markers. Signals of all three mass markers were detected in a UV-MALDI mass spectrum obtained from these beads. Relative signal intensities were found not to vary with the arrangement of the target sequences within the immobilized strand. * * * * *
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