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It is said that a memorial, whether monument or statue is a promise from the present to the future -- our posterity -- about the past. The true value of that memorial is determined by how well we keep the promise. What John Hagins recently did here with our hometown Maria Kirby-Smith in honoring Joseph Kershaw and Catawba Chief Hagler is simply outstanding! Powerful personalities from our town and county's birth on whom the public can more easily ponder. John stepped up on the mound when no one else did and Maria hit a homerun with these two characters cut realistically ...
Hugh Munn, a Camden native who I rarely worked with but respected greatly, died Oct. 19.
Obama's final debate with Mitt Romney wasn't nearly as fascinating as the debate that Romney appeared to be having with himself.
Thursday, October 18, 2012 was another day with another full-throated proposal for ethics reform from the political class in Columbia. This time, our governor heralded the creation of a "commission" (at least she doesn't label it "blue ribbon") to make "recommendations" to the General Assembly concerning "reform" of the current ethics laws.
Facebook is the answer to everything, and you better buy an iPad, folks, because the Internet is slowly, but surely taking over our lives.
Only an archivist/museum person gets excited about brand new acid-free boxes. I felt like it was Christmas when the shipping boxes from Gaylord Bros. Inc. began to arrive! Finally, the work of rehousing the collection in proper containers could begin. So, box by box, each collection was taken out of its old container and placed in new packaging. The database was checked for accuracy and the new boxes were placed back on the shelves in the stacks. All but this one box … as I peered inside to see what it contained, a little face with an agonizing expression peeked ...
It's time to lay the Electoral College to rest – or at least to alter it from a system that worked well in the 1700s but is hopelessly outdated now.
Mitt Romney's wince-inducing suggestion for gender equality, "binders full of women," has become almost as much of major Mitt-ism from the second presidential debate as "Big Bird" was in the first.
Unfortunately for former World No. 1 tennis player Caroline Wozniacki, her WTA success this year has come at the end of the season and not the beginning.
WASHINGTON -- Once again, Hillary Clinton has demonstrated herself to be the classiest person in the room. No wonder she's one of the most revered public figures in America.
Imagine waking up in the middle of the night from deadly smoke and fire alarms buzzing. You frantically wake your family and try to escape from the overwhelming black smoke. Feeling frazzled, you stumble down the stairs only to realize that the fire is blocking your escape out of the front door. What is your next step?
October 20, 1942.
Did the Taliban overplay their hand when they shot a 14-year-old Pakistani girl simply because she wanted to go to school? We can only hope.
Each of us has various benchmarks in our life.
When a person travels in a foreign country, he often uses the phrase, "Do you speak English?"
Hello, my name is Jimmy and I'm a hypochondriac.
Many people have crossed the path of my life, but only one crossed it from three different directions. Don Light, one of Nashville's most admired powerbrokers and star makers, was meant to be part of my life. I say this repeatedly because I encountered him through friends in country music, Southern gospel and NASCAR racing.
When we examine our experiences over time, our recollections of some of them stand out like posts supporting our "fence of life." These are memories we will never forget. Some refer to them as "muscle" memories, very strong ones.
By mid-June of 2000, I was so fed up and frustrated, I needed counseling.
WASHINGTON -- First-term first ladies are often shadows to their more-important husbands, dabbling in lite fare to avoid criticism and picking safe projects to shield them and their families from the inevitable slings and arrows.
Many extraordinary people offer visionary ideas, especially here. "Wouldn't it be great if we had a river rafting business on the Wateree?" "Wouldn't it be great if we had a downtown boutique hotel?" "Wouldn't it be great if we had a Bluegrass Festival the week of the Colonial Cup?" "Wouldn't it be great if we had a cottage development, or better yet, a new Kershaw County library on the former Mather property?" "And another restaurant or two!" The answer is predictably, "Yes, of course yes! Thank you for your great ideas," followed by necessary questions: "Where ...
CHARLESTON, S.C. -- By all appearances Friday morning, as thousands lined the street waiting (and wilting) for hours in 90-degree heat to enter the funeral arena where President Obama was to deliver a eulogy for state Sen. Clementa Pinckney, racial unity seemed a comfortable fact of life.
Even though it's not an election year, in many ways it's always an election year for some politicians. Given the fact they are "hired" and employed by the voting public, their lives are a nearly constant campaign for re-election. I can understand that. They have cushy jobs they want to keep for many years to come.
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Monitor, control, and install software on all the Mac computers on your network, without ever leaving your desk, using Apple Remote Desktop (ARD).
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Publications (482)2114.37 Total impact
Article: Differentiation between fresh and frozen-thawed sea bass (Dicentrarchus labrax) fillets using two-dimensional gel electrophoresis[Show abstract] [Hide abstract]
ABSTRACT: This study aimed to identify a protein marker that can differentiate between fresh skinless and frozen-thawed sea bass (Dicentrarchus labrax) fillets using the two-dimensional polyacrylamide gel electrophoresis (2-DE) technique. Distinct gel patterns, due to proteins with low molecular weight and low isoelectric points, distinguished fresh fillets from frozen-thawed ones. Frozen-thawed fillets showed two specific protein spots as early as the first day of the study. However, these spots were not observed in fresh fillets until at least 13 days of storage between 0 and 4°C, fillets were judged, beyond this period, fish were unfit for human consumption as revealed by complementary studies on fish spoilage indicators namely total volatile basic nitrogen and biogenic amines. Mass spectrometry identified the specific proteins as parvalbumin isoforms. Parvalbumins may thus be useful markers of differentiation between fresh and frozen-thawed sea bass fillets.Food Chemistry 06/2015; 176:294-301. DOI:10.1016/j.foodchem.2014.12.065
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ABSTRACT: Rod-derived cone viability factor (RdCVF) is an inactive thioredoxin secreted by rod photoreceptors that protects cones from degeneration. Because the secondary loss of cones in retinitis pigmentosa (RP) leads to blindness, the administration of RdCVF is a promising therapy for this untreatable neurodegenerative disease. Here, we investigated the mechanism underlying the protective role of RdCVF in RP. We show that RdCVF acts through binding to Basigin-1 (BSG1), a transmembrane protein expressed specifically by photoreceptors. BSG1 binds to the glucose transporter GLUT1, resulting in increased glucose entry into cones. Increased glucose promotes cone survival by stimulation of aerobic glycolysis. Moreover, a missense mutation of RdCVF results in its inability to bind to BSG1, stimulate glucose uptake, and prevent secondary cone death in a model of RP. Our data uncover an entirely novel mechanism of neuroprotection through the stimulation of glucose metabolism. Copyright © 2015 Elsevier Inc. All rights reserved.Cell 05/2015; 161(4):817-32. DOI:10.1016/j.cell.2015.03.023
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ABSTRACT: The high throughput characterization of protein N-termini is becoming an emerging challenge in the proteomics and proteogenomics fields. The present study describes the free N-terminome analysis of human mitochondria-enriched samples using trimethoxyphenyl phosphonium (TMPP) labeling approaches. Owing to the extent of protein import and cleavage for mitochondrial proteins, determining the new N-termini generated after translocation/processing events for mitochondrial proteins is crucial to understand the transformation of precursors to mature proteins. The dN-TOP strategy based on a double light/heavy TMPP labeling has been optimized in order to improve and automate the workflow for efficient, fast and reliable high throughput N-terminome analysis. A total of 2714 proteins were identified and 897 N-terminal peptides were characterized (424 N-α-acetylated and 473 TMPP-labelled peptides). These results allowed the precise identification of the N-terminus of 693 unique proteins corresponding to 26% of all identified proteins. Overall, 120 already annotated processing cleavage sites were confirmed while 302 new cleavage sites were characterized. The accumulation of experimental evidence of mature N-termini should allow increasing the knowledge of processing mechanisms and consequently also enhance cleavage sites prediction algorithms. Complete datasets have been deposited to the ProteomeXchange Consortium with identifiers PXD001521, PXD001522 and PXD001523. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.Proteomics 05/2015; DOI:10.1002/pmic.201400617
Article: Comparative Proteomic Analysis of the Molecular Responses of Mouse Macrophages to Titanium Dioxide and Copper Oxide Nanoparticles Unravels Some Toxic Mechanisms for Copper Oxide Nanoparticles in Macrophages[Show abstract] [Hide abstract]
ABSTRACT: Titanium dioxide and copper oxide nanoparticles are more and more widely used because of their catalytic properties, of their light absorbing properties (titanium dioxide) or of their biocidal properties (copper oxide), increasing the risk of adverse health effects. In this frame, the responses of mouse macrophages were studied. Both proteomic and targeted analyses were performed to investigate several parameters, such as phagocytic capacity, cytokine release, copper release, and response at sub toxic doses. Besides titanium dioxide and copper oxide nanoparticles, copper ions were used as controls. We also showed that the overall copper release in the cell does not explain per se the toxicity observed with copper oxide nanoparticles. In addition, both copper ion and copper oxide nanoparticles, but not titanium oxide, induced DNA strands breaks in macrophages. As to functional responses, the phagocytic capacity was not hampered by any of the treatments at non-toxic doses, while copper ion decreased the lipopolysaccharide-induced cytokine and nitric oxide productions. The proteomic analyses highlighted very few changes induced by titanium dioxide nanoparticles, but an induction of heme oxygenase, an increase of glutathione synthesis and a decrease of tetrahydrobiopterin in response to copper oxide nanoparticles. Subsequent targeted analyses demonstrated that the increase in glutathione biosynthesis and the induction of heme oxygenase (e.g. by lovastatin/monacolin K) are critical for macrophages to survive a copper challenge, and that the intermediates of the catecholamine pathway induce a strong cross toxicity with copper oxide nanoparticles and copper ions.PLoS ONE 04/2015; 10(4):e0124496. DOI:10.1371/journal.pone.0124496
Article: Characterization of the N-Terminal Heterogeneities of Monoclonal Antibodies Using In-Gel Charge Derivatization of α-Amines and LC-MS/MS[Show abstract] [Hide abstract]
ABSTRACT: The bioproduction of recombinant monoclonal antibodies (mAbs) results in complex mixtures of a main isoform and numerous macro and micro-variants. MAbs present therefore different levels of heterogeneities ranging from primary sequence variants to post-translational modifications. Among these heterogeneities are truncation and fragmentation of the primary amino-acid sequence resulting in shorter or cleaved polypeptide chains. Another reported heterogeneity comes from the incomplete processing of the signal peptide resulting in N-terminal elongated polypeptide chains. Here we present a novel in-gel protein N-terminal chemical derivatization method with trimethoxyphenyl phosphonium (TMPP) that enhances the mass spectrometry detection of N-terminal positions of proteins and allows their unambiguous assignment without altering the identification of internal digestion peptides. This method requires only one additional step to a classical peptide mapping workflow. This in-gel N-TOP (N-Terminal Oriented Proteomics) strategy was applied successfully to detect and characterize the N-terminal sequence heterogeneities of several monoclonal antibodies among which minor unexpected proteoforms.Analytical Chemistry 03/2015; 87(7). DOI:10.1021/ac504427k
Article: Native mass spectrometry and ion mobility characterization of trastuzumab emtansine, a lysine-linked antibody drug conjugate: Native MS and IM-MS for Trastuzumab Emtansine Analysis[Show abstract] [Hide abstract]
ABSTRACT: Antibody-drug conjugates (ADCs) are bio-chemotherapeutics consisting of a cytotoxic chemical drug linked covalently to a monoclonal antibody. Two main classes of ADCs, namely cysteine and lysine conjugates, are currently available on the market or involved in clinical trials. The complex structure and heterogeneity of ADCs makes their biophysical characterization challenging. For cysteine conjugates, hydrophobic interaction chromatography is the gold standard technique for studying drug distribution, the naked antibody content, and the average drug to antibody ratio (DAR). For lysine ADC conjugates on the other hand, which are not amenable to hydrophobic interaction chromatography because of their higher heterogeneity, denaturing mass spectrometry (MS) and UV/Vis spectroscopy are the most powerful approaches. We report here the use of native MS and ion mobility (IM-MS) for the characterization of trastuzumab emtansine (T-DM1, Kadcyla®). This lysine conjugate is currently being considered for the treatment of human epidermal growth factor receptor 2 (HER2)-positive breast cancer, and combines the anti-HER2 antibody trastuzumab (Herceptin®), with the cytotoxic microtubule-inhibiting maytansine derivative, DM1. We show that native MS combined with high-resolution measurements and/or charge reduction is beneficial in terms of the accurate values it provides of the average DAR and the drug load profiles. The use of spectral deconvolution is discussed in detail. We report furthermore the use of native IM-MS to directly determine DAR distribution profiles and average DAR values, as well as a molecular modeling investigation of positional isomers in T-DM1. This article is protected by copyright. All rights reserved. © 2015 The Protein Society.Protein Science 02/2015; DOI:10.1002/pro.2666
Article: Combining native MS approaches to decipher archaeal box H/ACA ribonucleoprotein particle structure and activity[Show abstract] [Hide abstract]
ABSTRACT: Site-specific isomerization of uridines into pseudouridines in RNAs is catalyzed either by stand-alone enzymes or by box H/ACA ribonucleoprotein particles (sno/sRNPs). The archaeal box H/ACA sRNPs are 5-component complexes that consist of a guide RNA and the aCBF5, aNOP10, L7Ae and aGAR1 proteins. In this study, we performed pairwise incubations of individual constituents of archaeal box H/ACA sRNPs and analyzed their interactions by native mass spectrometry (MS) to build a 2D connectivity map of direct binders. We describe the use of native MS in combination with ion mobility-MS to monitor the in vitro assembly of the active H/ACA sRNP particle. Real-time native MS was used to monitor how box H/ACA particle functions in multiple-turnover conditions. Native MS also unambiguously revealed that a substrate RNA containing 5-fluorouridine (f(5) U) was hydrolyzed into 5-fluoro-6-hydroxy-pseudouridine (f(5) ho(6) Ψ). In terms of enzymatic mechanism, box H/ACA sRNP was shown to catalyze the pseudouridylation of a first RNA substrate, then to release the RNA product (S22 f(5) ho(6) ψ) from the RNP enzyme and reload a new substrate RNA molecule. Altogether, our native MS-based approaches provide relevant new information about the potential assembly process and catalytic mechanism of box H/ACA RNPs. This article is protected by copyright. All rights reserved. This article is protected by copyright. All rights reserved.Proteomics 02/2015; DOI:10.1002/pmic.201400529
Article: Cutting-edge mass spectrometry characterization of originator, biosimilar and biobetter antibodies[Show abstract] [Hide abstract]
ABSTRACT: The approval process for antibody biosimilars relies primarily on comprehensive analytical data to establish comparability and high similarity with the originator. Mass spectrometry (MS) in combination with liquid chromatography (LC) and electrophoretic methods are the corner stone for comparability and biosimilarity evaluation. In this special feature we report head-to-head comparison of trastuzumab and cetuximab with corresponding biosimilar and biobetter candidates based on cutting-edge mass spectrometry techniques such as native MS and ion-mobility MS at different levels (top, middle and bottom). In addition, we discuss the advantages and the limitations of sample preparation and enzymatic digestion, middle-up and -down strategies and the use of hydrogen/deuterium exchange followed by MS (HDX-MS). Last but not least, emerging separation methods combined to MS such as capillary zone electrophoresis-tandem MS (CESI-MS/MS), electron transfer dissociation (ETD), top down-sequencing (TDS) and high-resolution MS (HR-MS) that complete the panel of state-of-the-art MS-based options for comparability and biosimilarity evaluation are presented. Copyright © 2015 John Wiley & Sons, Ltd. Copyright © 2015 John Wiley & Sons, Ltd.Journal of Mass Spectrometry 02/2015; 50(2). DOI:10.1002/jms.3554
Article: CBTF: New Amine-to-Thiol Coupling Reagent for Preparation of Antibody Conjugates with Increased Plasma Stability[Show abstract] [Hide abstract]
ABSTRACT: Amine-to-thiol coupling is the most common route for the preparation of antibody-drug conjugates (ADC). It is usually achieved by using heterobifunctional reagents possessing an activated ester at one end and a maleimide group at the other. However, maleimide-based conjugates were recently revealed to have limited stability in blood circulation, which can compromise therapeutic efficacy of the conjugate. To address this issue, we have developed a heterobifunctional reagent, sodium 4-((4-(cyanoethynyl)benzoyl)oxy)-2,3,5,6-tetrafluorobenzenesulfonate (CBTF), for amine-to-thiol coupling. It comprises a recently described 3-arylpropionitrile (APN) function in replacement of maleimide and allows for the preparation of remarkably stable conjugates. A series of antibody-dye conjugates have been prepared using this reagent and shown superior stability in human blood plasma compared to maleimide-derived conjugates.Bioconjugate Chemistry 01/2015; 26(2). DOI:10.1021/bc500610g
Article: TG1050, an immunotherapeutic to treat chronic hepatitis B, induces robust T cells and exerts an antiviral effect in HBV-persistent mice.[Show abstract] [Hide abstract]
ABSTRACT: To assess a new adenovirus-based immunotherapy as a novel treatment approach to chronic hepatitis B (CHB). TG1050 is a non-replicative adenovirus serotype 5 encoding a unique large fusion protein composed of a truncated HBV Core, a modified HBV Polymerase and two HBV Envelope domains. We used a recently described HBV-persistent mouse model based on a recombinant adenovirus-associated virus encoding an over length genome of HBV that induces the chronic production of HBsAg, HBeAg and infectious HBV particles to assess the ability of TG1050 to induce functional T cells in face of a chronic status. In in vitro studies, TG1050 was shown to express the expected large polyprotein together with a dominant, smaller by-product. Following a single administration in mice, TG1050 induced robust, multispecific and long-lasting HBV-specific T cells detectable up to 1 year post-injection. These cells target all three encoded immunogens and display bifunctionality (ie, capacity to produce both interferon γ and tumour necrosis factor α as well as cytolytic functions). In addition, control of circulating levels of HBV DNA and HBsAg was observed while alanine aminotransferase levels remain in the normal range. Injection of TG1050 induced both splenic and intrahepatic functional T cells producing cytokines and displaying cytolytic activity in HBV-naïve and HBV-persistent mouse models together with significant reduction of circulating viral parameters. These results warrant clinical evaluation of TG1050 in the treatment of CHB. Published by the BMJ Publishing Group Limited. For permission to use (where not already granted under a licence) please go to http://group.bmj.com/group/rights-licensing/permissions.Gut 11/2014; DOI:10.1136/gutjnl-2014-308041
Article: Innovative Native MS Methodologies for Antibody Drug Conjugate Characterization: High Resolution Native MS and IM-MS for Average DAR and DAR Distribution Assessment[Show abstract] [Hide abstract]
ABSTRACT: Antibody drug conjugates (ADCs) are macromolecules composed of cytotoxic drugs covalently attached via a conditionally stable linker to monoclonal antibodies (mAbs). ADCs are among most promising next generation of empowered mAbs foreseen to treat cancers. Compared to naked mAbs, ADCs have increased level of complexity as the heterogeneity of conjugation cumulates with the inherent micro-variability of the biomolecule. An increasing need underlying ADC's development and optimization is to improve its analytical and bioanalytical characterization by assessing three main ADC quality attributes: drug distribution, amount of naked antibody and average drug to antibody ratio (DAR). Here, the analytical potential of native mass spectrometry (MS) and native ion mobility MS (IM-MS) is compared to hydrophobic interaction chromatography (HIC), the reference method for quality control of interchain cysteinyl-linked ADCs. Brentuximab vedotin, first in class and gold standard, was chosen for a proof of principle. High resolution native MS provided accurate mass measurement (<30 ppm) of intact ADCs together with average DAR and drug distribution, confirming the unique ability of native MS for simultaneous detection of mixtures of covalent and non-covalent products. Native IM-MS was next used for the first time to characterize an ADC. IM-MS evidenced ADC multiple drug loading, collisional cross sections measurement of each payload species attesting slight conformational changes. A semi-quantitative interpretation of IM-MS data was developed to directly extrapolate average DAR and DAR distribution. Additionnaly, HIC fractions were collected and analyzed by native MS and IM-MS, assessing the interpretation of each HIC peak. Altogether, our results illustrate how native MS and IM-MS can rapidly assess ADC structural heterogeneity and how easily these methods can be implemented into MS workflows for in-depth ADC analytical characterization.Analytical Chemistry 09/2014; 86(21). DOI:10.1021/ac502593n
Article: Protein Hit1, a novel box C/D snoRNP assembly factor, controls cellular concentration of the scaffolding protein Rsa1 by direct interaction[Show abstract] [Hide abstract]
ABSTRACT: Biogenesis of eukaryotic box C/D small nucleolar ribonucleoprotein particles (C/D snoRNPs) involves conserved trans-acting factors, which are proposed to facilitate the assembly of the core proteins Snu13p/15.5K, Nop58p/NOP58, Nop56p/NOP56 and Nop1p/Fibrillarin on box C/D small nucleolar RNAs (C/D snoRNAs). In yeast, protein Rsa1 acts as a platform, interacting with both the RNA-binding core protein Snu13 and protein Pih1 of the Hsp82-R2TP chaperone complex. In this work, a proteomic approach coupled with functional and structural studies identifies protein Hit1 as a novel Rsa1p-interacting partner involved in C/D snoRNP assembly. Hit1p contributes to in vivo C/D snoRNA stability and pre-RNA maturation kinetics. It associates with U3 snoRNA precursors and influences its 3'-end processing. Remarkably, Hit1p is required to maintain steady-state levels of Rsa1p. This stabilizing activity is likely to be general across eukaryotic species, as the human protein ZNHIT3(TRIP3) showing sequence homology with Hit1p regulates the abundance of NUFIP1, the Rsa1p functional homolog. The nuclear magnetic resonance solution structure of the Rsa1p317-352-Hit1p70-164 complex reveals a novel mode of protein-protein association explaining the strong stability of the Rsa1p-Hit1p complex. Our biochemical data show that C/D snoRNAs and the core protein Nop58 can interact with the purified Snu13p-Rsa1p-Hit1p heterotrimer.Nucleic Acids Research 08/2014; 42(16). DOI:10.1093/nar/gku612
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ABSTRACT: Complete starvation may prove lethal due to excessive loss of body proteins. However, it is still not completely understood whether responses to food deprivation are time-dependently induced or triggered in relation with the successive phases of protein sparing and wasting that characterize prolonged fasting. As the liver has a wide range of vital functions, we examined the hepatic regulatory mechanisms elicited during prolonged fasting. We showed that fasting-induced transcriptome/proteome changes occur in close relation with fuel partitioning, independently of ATP levels. Omics data suggesting a worsening of oxidative stress during the proteolytic stage of fasting, this was further validated using biochemical assays. Low levels of antioxidant factors were indeed paralleled by their decreased activity, which could be impaired by low NADPH levels. Oxidative damages on lipids and proteins were accordingly increased only during late fasting. At this stage, the gene/protein expression of several chaperones was also repressed. Together with the impairment of metabolic achievements, a vicious cycle involving protein misfolding and oxidative stress could jeopardize liver functions when the proteolytic stage of fasting is reached. Thus, monitoring of liver impairments should help to better manage or treat catabolic and/or oxidative stress conditions, such as ageing and degeneration. This article is protected by copyright. All rights reserved.Proteomics 08/2014; 14(16). DOI:10.1002/pmic.201400051
Article: Flowering as the Most Highly Sensitive Period of Grapevine (Vitis vinifera L. cv Mourvèdre) to the Botryosphaeria Dieback Agents Neofusicoccum parvum and Diplodia seriata Infection[Show abstract] [Hide abstract]
ABSTRACT: Botryosphaeria dieback is a fungal grapevine trunk disease that currently represents a threat for viticulture worldwide because of the important economical losses due to reduced yield of affected plants and their premature death. Neofusicoccum parvum and Diplodia seriata are among the causal agents. Vine green stems were artificially infected with N. parvum or D. seriata at the onset of three different phenological stages (G stage (separated clusters), flowering and veraison). Highest mean lesion lengths were recorded at flowering. Major proteome changes associated to artificial infections during the three different phenological stages were also reported using two dimensional gel electrophoresis (2D)-based analysis. Twenty (G stage), 15 (flowering) and 13 (veraison) differentially expressed protein spots were subjected to nanoLC-MS/MS and a total of 247, OPEN ACCESS Int. J. Mol. Sci. 2014, 15 9645 54 and 25 proteins were respectively identified. At flowering, a weaker response to the infection was likely activated as compared to the other stages, and some defense-related proteins were even down regulated (e.g., superoxide dismutase, major latex-like protein, and pathogenesis related protein 10). Globally, the flowering period seemed to represent the period of highest sensitivity of grapevine to Botryosphaeria dieback agent infection, possibly being related to the high metabolic activity in the inflorescences.International Journal of Molecular Sciences 05/2014; 15(6):9644-9669. DOI:10.3390/ijms15069644
Article: Litter size manipulation in laboratory mice: an example of how proteomic analysis can uncover new mechanisms underlying the cost of reproduction[Show abstract] [Hide abstract]
ABSTRACT: Background Life history theories predict that investment in current reproduction comes at a cost for future reproduction and survival. Oxidative stress is one of the best documented mechanisms underlying costs of reproduction to date. However, other, yet to be described molecular mechanisms that play a short term role during reproduction may explain the negative relationships underlying the cost of reproduction. To identify such new mechanisms, we used a global proteomic determination of liver protein profiles in laboratory adult female mice whose litter size had been either reduced or enlarged after birth. This litter size manipulation was expected to affect females by either raising or decreasing their current reproductive effort. At the same time, global parameters and levels of oxidative stress were also measured in all females. Results Based on plasma analyses, females with enlarged litters exhibited increased levels of oxidative stress at the date of weaning compared to females with reduced litters, while no significant difference was found between both the latter groups and control females. None of the liver proteins related to oxidative balance were significantly affected by the experimental treatment. In contrast, the liver protein profiles of females with enlarged and reduced litters suggested that calcium metabolism and cell growth regulation were negatively affected by changes in the number of pup reared. Conclusions Plasma oxidative stress levels in reproductive mice revealed that the degree of investment in reproduction can actually incur a cost in terms of plasmatic oxidative stress, their initial investment in reproduction being close to maximum and remaining at a same level when the energy demand of lactation is reduced. Liver proteomic profiles in reproductive females show that hepatic oxidative stress is unlikely to be involved in the cost of reproduction. Reproductive females rather exhibited liver protein profiles similar to those previously described in laboratory ageing mice, thus suggesting that hepatic cell pro-ageing processes may be involved in the cost of reproduction. Overall, our data illustrate how a proteomic approach can unravel new mechanisms sustaining life-history trade-offs, and reproduction costs in particular.Frontiers in Zoology 05/2014; 11:41. DOI:10.1186/1742-9994-11-41
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ABSTRACT: Among fungal grapevine trunk diseases, esca proper poses a significant threat for viticulture. Apoplexy, mainly occurring on grapevines affected by esca proper, is also a threat. To verify if different responses are activated in the woody tissues of apoplectic (A) and esca proper-affected (E) vines, two-dimensional gel electrophoresis coupled to mass spectrometry analysis was used to examine changes in the trunk wood of E and A field-grown plants. Asymptomatic and black streaked trunk (BST) wood from A and E plants were compared to asymptomatic and BST wood of control plants. Twenty-seven differentially expressed protein spots were identified. For eleven targeted proteins, expression of the relative transcripts was also monitored by qRT-PCR. Hierarchical tree clustering revealed differences in the distribution of spots containing carbohydrate metabolism proteins and heat shock proteins between asymptomatic- and BST-wood of grapevine, irrespective of the type of plant examined (control or diseased grapevines). Asymptomatic wood was mainly characterized by down-expression of proteins involved in cell growth and defense responses. The proteome of BST wood, characterized by extensive presence of grapevine trunk disease agents, revealed over-expression of proteins involved in defense. There was no evidence of strong different response in the trunk wood of apoplectic and esca proper affected plants. This could mean that, despite the different feature of these external crown symptoms, grapevine responses at trunk wood level is very similar in both cases. This plant response might therefore either simply be due to the fact that plants can react in the same way to different stresses, or that apoplexy represents a different effect provoked by the same cause or causal agent(s).Phytopathologia Mediterranea 05/2014; 53(1):168-187. DOI:10.14601/Phytopathol_Mediterr-12913
Article: Enzymatic cocktails produced by Fusarium graminearum under submerged fermentation using different lignocellulosic biomasses[Show abstract] [Hide abstract]
ABSTRACT: Fusarium graminearum was grown on four lignocellulosic substrates (corn cobs, wheat bran, hop cell walls, and birchwood) and glucose as the sole carbon source. Proteomic studies performed on the resulting enzymatic cocktails highlighted a great diversity in the number and type of proteins secreted. The cell wall degrading enzymes (CWDE) proportion varied greatly from 20% to 69%. Only 1 of the 57 CWDEs detected in this study was common to the five proteomes. In contrast, 35 CWDEs were specific to one proteome only. The polysaccharide-degradation activities were different depending on the cocktail and the polysaccharide used. F. graminearum strongly modifies the enzymatic cocktail it secretes as a function of the biomass used for growth. This article is protected by copyright. All rights reserved.FEMS Microbiology Letters 05/2014; 355(2). DOI:10.1111/1574-6968.12467
Article: MSDA, a proteomics software suite for in-depth Mass Spectrometry Data Analysis using grid computing[Show abstract] [Hide abstract]
ABSTRACT: One of the major bottlenecks in the proteomics field today resides in the computational interpretation of the massive data generated by the latest generation of high-throughput mass spectrometry instruments. MS/MS datasets are constantly increasing in size and complexity and it becomes challenging to comprehensively process such huge datasets and afterwards deduce most relevant biological information. The Mass Spectrometry Data Analysis (MSDA, https://msda.unistra.fr) online software suite provides a series of modules for in-depth MS/MS data analysis. It includes a custom databases generation toolbox, modules for filtering and extracting high-quality spectra, for running high performance database and de novo searches, and for extracting modified peptides spectra and functional annotations. Additionally, MSDA enables running the most computationally intensive steps, i.e. database and de novo searches, on a computer grid thus providing a net time gain of up to 99% for data processing. This article is protected by copyright. All rights reserved.Proteomics 05/2014; 14(9). DOI:10.1002/pmic.201300415
Article: Analysis of cellular responses of macrophages to zinc ions and zinc oxide nanoparticles: a combined targeted and proteomic approach[Show abstract] [Hide abstract]
ABSTRACT: Two different zinc oxide nanoparticles, as well as zinc ions, are used to study the cellular responses of the RAW 264 macrophage cell line. A proteomic screen is used to provide a wide view of the molecular effects of zinc, and the most prominent results are cross-validated by targeted studies. Furthermore, the alteration of important macrophage functions (e.g. phagocytosis) by zinc is also investigated. The intracellular dissolution/uptake of zinc is also studied to further characterize zinc toxicity. Zinc oxide nanoparticles dissolve readily in the cells, leading to high intracellular zinc concentrations, mostly as protein-bound zinc. The proteomic screen reveals a rather weak response in the oxidative stress response pathway, but a strong response both in the central metabolism and in the proteasomal protein degradation pathway. Targeted experiments confirm that carbohydrate catabolism and proteasome are critical determinants of sensitivity to zinc, which also induces DNA damage. Conversely, glutathione levels and phagocytosis appear unaffected at moderately toxic zinc concentrations.Nanoscale 05/2014; 6(11). DOI:10.1039/c4nr00319e
- [Show abstract] [Hide abstract]
ABSTRACT: Botryosphaeria dieback is a fungal grapevine trunk disease which represents a threat for viticulture worldwide due to the decreased production of affected plants and their premature death. This dieback is characterized by a typical wood discoloration called "brown stripe". Herein, a proteome comparison of the brown striped wood from Botryosphaeria dieback-affected standing vines cultivar 'Chardonnay', 'Gewurztraminer' and 'Mourvèdre' was performed. The transcript analysis for 15 targeted genes and the quantification of both total phenolics and specific stilbenes were also performed. Several pathogenesis-related proteins and members of the antioxidant system were more abundant in the brown striped wood of the three cultivars, whereas other defense-related proteins were less abundant. Additionally, total phenolics and some specific stilbenes were more accumulated in the brown striped wood. Strongest differences among the cultivars concerned especially proteins of the primary metabolism, which looked to be particularly impaired in the brown striped wood of 'Chardonnay'. Low abundance of some proteins involved in defense response probably contributes to make global response insufficient to avoid the symptom development. The differential susceptibility of the three grapevine cultivars could be linked to the diverse expression of various proteins involved in defense response, stress tolerance and metabolism.Phytopathology 04/2014; 104(10). DOI:10.1094/PHYTO-01-14-0007-R
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French Institute of Health and Medical ResearchLutetia Parisorum, Île-de-France, France
University of Geneva
Genève, Geneva, Switzerland
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European Molecular Biology LaboratoryHeidelburg, Baden-Württemberg, Germany
Universität Basel
Basel, BS, Switzerland
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Natural Product Chemistry InstituteLutetia Parisorum, Île-de-France, France
Odense University Hospital
Odense, South Denmark, Denmark
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TransgeneIllkirch, Alsace, France


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NOVA scienceNOW
Series 4, Episode 7 (#407)
Repairs on the Hubble space telescope and the link between brain structure and memory are featured. [56 minutes]
This episode has not aired in the past few months on Iowa Public Television.
NOVA scienceNOW | Saving Hubble Update, Gangster... (#407)
Full Program: The repair mission for the Hubble space telescope, as astronauts try to fix crucial equipment that was not designed... 52:47
NOVA scienceNOW | A Memorable Snail | PBS (#407)
In this short video, UCLA's David Glanzman shows what a spineless sea snail can teach us about our brains. 04:02
NOVA scienceNOW | What Are Animals Thinking? | PBS (#605)
Full Program: Probe animal morality, the "swarm intelligence" of a beehive, and more. 53:37
What Are Animals Thinking? | NOVA scienceNOW | PBS
NOVA scienceNOW: What Are Animals Thinking? premieres November 7th at 10PM/9c on PBS. Visit our website at... 00:31
NOVA scienceNOW | Can I Eat That? | PBS (#604)
Full Program: Learn about the neuroscience of taste, what makes a Thanksgiving turkey savory, and more. 53:37
- Watch more video from this program »
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It'Äôs¬ÝChristmas in the Sixth Dimension!¬Ýas director Richard Elfman returns to Long Beach with his 1982 manic musical masterpiece¬ÝForbidden Zone'Äì just in time for Christmas, and with a few Holiday surprises!
Notable as being the very first Mondo Midnight selection at The Art Theatre of Long Beach, FORBIDDEN ZONE¬Ýis the inspired brainchild of Richard Elfman, founding member of the legendary Mystic Knights of the Oingo Boingo, who along with writer Matthew Bright (who would later helm the 1996 cult classic¬ÝFreeway) mixed in everything from Max Fleisher to Cab Calloway to Josephine Baker to unleash a musical unlike any the world had ever seen. Mix in a typically masterful score by Elfman brother Danny (who makes a fan-favorite appearance'Ķ), a dancing frog in a tuxedo, a gorilla, an ex-queen(!!?), and a crazed kingdom ruled by the weirdest royal family in celluloid history, and you have the movie that Film Threat called'ÄúThe Citizen Kane of Underground Movies.'Äù
By popular demand, Cell 63 will be returning with their LIVE SHADOWCAST! ¬ÝAfter rocking the Art Theatre in April with the first¬ÝForbidden Zone¬Ýshadowcast¬Ýever, ¬Ýthe talented performers of Cell 63 'Äì featuring members of¬ÝRocky Horror Picture Show¬Ýcasts from both Long Beach and Los Angeles! -¬Ýare ready to take the stage once again in the ultimate tribute to Elfman'Äôs wonderfully wicked vision!
And yes – they have some Holiday surprises up their sleeves…
Tickets on sale at the Art Theatre box office, or¬Ýonline by clicking here.
Cinematheque Members¬Ý'Äì show you card at the Art Theatre box office and save $2 per ticket!
Please help us spread the word 'Äì¬ÝRSVP and Share this event on Facebook!


text: so So SO ⋰ Alex Blair Los Angeles / San Diego, United States Down to collab. I'm also a graphic artist, animator, and student. So if you need a logo, I might be able to help! ⋰⋰⋰⋰⋰⋰⋰⋰⋰⋰⋰⋰⋰⋰⋰⋰⋰⋰⋰⋰⋰⋰⋰⋰⋰⋰⋰ Bookings and inquiries: firstname.lastname@example.com ⋱⋱⋱⋱⋱⋱⋱⋱⋱⋱⋱⋱⋱⋱⋱⋱⋱⋱⋱⋱⋱⋱⋱⋱⋱⋱⋱ I don't usually try to produce anything genre specific. I just grab sounds and go. All is experimental (as music should be). 96 Users who follow so So SO ⋰


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EU approves common patent rules
Still, a common patent system, despite some flaws, should hold huge advantages over the national system as it exists now. Patents protect the rights of inventors and are seen as a key to fuel creativity and innovation.
EU officials said that the current complicated EU patent approval now costs about €36,000 ($46,800) compared to some €1,800 ($2,300) in the United States and some €600 ($800) in China.
The U.N.'s World Intellectual Property Organization highlighted on Tuesday how the EU is lagging behind on patents.
China's patent office received 526,412 applications in 2011, ahead of the United States with 503,582 filings. In contrast, the European patent office had 142,793 applications in 2011, down 5.4 percent from 2010.
"One of the reasons for this difference is without a doubt the prohibitive cost and the complexity of obtaining patent protection," he said.
Under the new rules, patenting costs could come down by up to 80 percent once the system kicks in at the beginning of 2014, said Cecilia Wikstrom, of the ALDE liberal group in the European Parliament.
"A common European patent is key to strengthening Europe's competitiveness in a globalized world. We must be able to compete with the U.S, Japan and other developed countries,"Wikstrom said.


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A model for the viscous dissipation rate in stably stratified, sheared turbulence
[1] A model for the turbulence dissipation rate in stably stratified shear turbulence is developed and validated. The functional dependence of the model is derived from first principles and it represents a conceptually new approach in that it depends on the background temperature field rather than on the fluctuating velocity field. This novel feature makes the proposed model a viable candidate for dissipation rate estimates in measured real-life flows. Direct numerical simulation data are used for a priori assessment of the proposed model. It is demonstrated that the proposed model performs very well, particularly in cases where the background stratification becomes dynamically important. Also, a generalized expression for the mixing coefficient has been rigorously derived from first principles assuming local isotropy of incompressible turbulent flows. The mixing coefficient is shown to depend on the Prandtl number and values are in correspondence with previous studies.
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Friday, June 5, 2009
"It's So Hard To Say Goodbye"
From left: some of the Mag.Com staff. See ya Monday, friends.
about sums up today. Thing is, we ain't disappearing. We
. Smart + fun = cool. On the first day (
one sweet day
?) of this website's existence, our boss was locked out of the office and we were desperately trying to get a pic of
Devin Harris
to crop properly (we were '
on bended knee
,' if you will). It's been a magical ride since then, if by "magical" you mean something else that doesn't really mean "magical." (We kid.) To everyone involved -- C, S, M, D, N, S, L, S, J, E, B, S, R (we protect full names) -- keep grinding on the pay wall side.
Water never runs dry
content-wise, and yes, simply,
thank you.


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Oct 31, 2008 | 12:07 PM CDT
Croda Dedicates Skin Care, Industrial R&D Centers
Croda Inc. announced the official opening and dedication of two new innovation centers--the Atlas Point Commercial Center for skin care, and the William C. Griffin Innovation Center for industrial R&D labs.
Oct 31, 2008 | 09:40 AM CDT
The Body Shop Names President for Americas Region
Kowalczyk responsible for the company's growth in the US, Canada and Mexico; ability to develop sustainable business models cited.
Oct 17, 2008 | 09:32 AM CDT
P&G Recognizes Top Performing Suppliers
Supplier Summit brings focus to continued collaboration; company cites suppliers as "critical partners" in realizing innovations and achieving sustainability goals and growth.
Oct 14, 2008 | 12:36 PM CDT
Motivation by Career Development
By: James M. Wilmott
A career development program based on a new interpretation of the dual ladder concept may take the mystery out of the promotion process and enable employees to take control of their career aspirations.
Oct 14, 2008 | 11:43 AM CDT
First Impressions
By: Sara Mason
Innovation in cosmetic sample packaging allows marketers to convey the benefits, attributes and value of a product brand to the consumer.
Oct 14, 2008 | 11:39 AM CDT
Green is Sexy
By: Nancy Jeffries
Long-lasting, hydrating, high-tech, socially responsible and more—the market for color has brand new dimensions.
Oct 13, 2008 | 04:57 PM CDT
Launch Sequence
By: Karen A. Newman
Marjorie Kitzrow has put a 30-year-career's worth of marketing and brand building experience into the launch of Summersent, the first prestige fragrance for women to be launched by her company, Marjorie Midgarden Fragrances. The company grew from the memorable aroma of a single bloom into a firm dedicated to the creation and marketing of fine fragrance and related products through premium channels.
Oct 13, 2008 | 04:42 PM CDT
Trade Routes: Learning to Swim in the Global Market
By: Michael Wynne
The global market is filled with opportunities that require action—and in today's fiercely competitive business world, you can't afford to ignore any potential market.
Oct 13, 2008 | 04:39 PM CDT
Innovation Drives Sales Growth
By: Imogen Matthews
Product innovation is the main driver of sales growth in the cosmetics and toiletries market as brands compete to achieve maximum standout on shelf and aim to win over new customers.
Oct 13, 2008 | 04:26 PM CDT
10 Ways to Get Your Products Noticed by Salons
By: Sara Mason
From partnerships to philanthropy to very important education opportunities, if your target market is the salon industry, here are 10 great ways to get noticed and get your product into stylists' hands.


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"Dear Steve, I'm 35, new dad, have a terrible credit score(in the 400s), want to improve my score for a better quality of life, and most of my debts are 8 years and older. I want to find specific information on how to remove old debts from my credit report. (ex. To whom do I write my letters? What should ... Read More »
My Brother is Being Sued by American Express. What Can He Do About It? – Ryan
"Dear Steve, My brother is being sued by American Express. He had a card with them and had about 2 to 3 K of dept on the card. Then he lost his job and got a divorce. He has note been able to make payments or pay it off. As he has no income. What can he do about his. ... Read More »
What Do I Do to Consolidate Bills? Mary
"Dear Steve, Need help with consolidating bills. What do I have to do in order to start this process? Mary" I asked my friend Mike Killian to answer your question for you. I wanted to make sure you got an answer as quickly as possible as I'm a bit backed up at the moment. I'll be watching the comments on ... Read More »
We Own Two Homes. Should We Try to Get a Loan Modification Instead of Refinancing? – Esty
"Dear Steve, We own two homes. One is our permanent primary residence in TN. The other is our smaller previous residence in (here it comes…) Florida. Yes, Florida. Yay. We bought in 2005 with a 5/1 ARM. We have until May 2010 to refinance that first 5 year ARM. We have had tenants there this year who will move out ... Read More »
Credit Card Company Put Someone Else's Name On My Account- Help – Heather
"Dear Steve, Discrepencies on credit report and credit card mess. Question The cc company accidentally put someone elses name on myaccount. I couldn't have access to the account while they investigated and when the reopened it there were tons of late fees and penelties. It was not my fault. Someone opened a lot of credit using my info, need help ... Read More »
Deceased Spouse Left $33,000 Worth of Credit Card Debt. What Can I Do? – Judy
"Dear Steve, My husband passed away rhe end of 2008. I had been iill in 2007 and was not working. Other than the horrible fact he died, I did not know we were in financial trouble. Two days after he died, I opened mail I had never seen and found out he had been carrying close to $30,000 in credit ... Read More »
What Can I Do With the Payday Loan Vicious Cycle? – Dodie
"Dear Steve, Payday loan overload. I am broke and getting worse every payday. I am in the vicious cycle of payday loans and i am delinquent on some and they are threatening me with garnishments and employer contact. I can not seem to get out of this debt cycle and it takes all my paycheck to pay interest that I ... Read More »
I Need an Honest and Straightforward Answer. – Andra
"Dear Steve, Three years ago my husband and I moved back to Los Angeles for me to pursue my dream of owning my own clothing business (with a partner). We sold our home in 2003 and make 200K. Moved to Colorado bought a bigger house for 380K disliked CO sold it for the same amount, moved to NV bought a ... Read More »
Unemployed, Pregnant, and Can't Afford the Payments – Erin
"Dear Steve, Unemployed, pregnant, and cant afford the payments demanded by the debt collectors. I am trying to make the demanded payments by the debt collectors, with out defaulting on my car payment, insurance, and medical bills. Do I have to send the collection agency the payment they are demanding, or can I send what little I can afford without ... Read More »
What Do You Think of the Dave Burke Real Talk System of Eliminating Debt? – Stan
"Dear Steve, I am a very underemployed real estate broker of 35 years. Have you heard of Dave Burke and Real Talk and his secret system of paying off debt for only $1997? Stan" Dear Stan, I had not heard of Dave Burke or his Real Talk show or the $1997 price for some magic debt elimination scheme. First, ... Read More »
I Let a Friend Borrow 13,000 Dirhams and I Want it Back Before He Leaves the Country. – Sarah
"Dear Steve, hi, i lend a money to my friend for like 6 months that reach about 13,000 dirhams. a few weeks ago i heard that he is going to have his annual vacation. that makes me a bot worried. coz there are rumors that he might not come back after his leave. so i talked to him and asked ... Read More »
Creditor Jurisdiction Overseas – Jonny
"Dear Steve, Moved overseas to New Zealand a couple years ago. Went overseas with about $8000USD in credit card debt. I've tried to pay it off but a couple "life accidents" with employers have left me at about the same amount. I'm setled in the South Pacific and have no intention of returning to the US to live. I had ... Read More »
Is There a Way to Get Out of Debt for Free? – Steve
"Dear Steve, I was unemployed for 7 months in 2007 and moved to Texas for employment, The company shut down the project in Novemeber of 2007 then fired 35 people in January of 08. During this time My wife lived in New Jersey and never came to Houston and filed for divorce. I never thought that New Jersey would award ... Read More »
Can I Refinance My Car or Consolidate All Debt? – Rose
"Dear Steve, I have about $45K worth of debt. I have four credit cards with balances of $500 each. I have IRS debt of $1600 and $3000 balance on Best buy card. And a $38K auto loan. Truthfully, its the auto loan that is killing me. I pay 13% interest. SO now my loan is upside down. its higher then ... Read More »
I'm in a Forced Marriage in the United Arab Emirates and Deep in Debt. – Abdulrazak
"Dear Steve, Well i really dont know where to start.recently married around 6 months ago..it was a forced marriage..now i have put myself in such heavy debt that i am left with nothing at all each month and still bills to pay..i have 2 credit cards over limit..totaling up to $ 11,444 And a car loan $46 000..im currently earnin ... Read More »


text:
This profile for Golden West Properties is located in Los Angeles, CA. Golden West Properties industry is listed as Developer and Operator of Commercial Buildings.
Michael Pashaie serves as the Partner and has interests in other corporate entities including Flower Development, LLC, Sunset Hilldale LLC and twenty-four more corporations. Michael's past corporate affiliations include Pashaie & Shooshani, Inc., Ptm Management, Inc. and nine others.
Tom Pashaie is the Partner of Golden West Properties. Tom's additional corporate interests include Flower Development, LLC located in West Hollywood, CA . Tom's past corporate affiliations include Nevada Horizons Holdings, LLC .
Key roles for Golden West Properties
Michael Pashaie
Partner Owner
Tom Pashaie
Comprehensive Insight Plus Report
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|The D&B Comprehensive Insight Plus Report for Golden West Properties combines D&B's proprietary statistical scoring with business, payment and financial information all in one report.
US Company Profile Report
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|The D&B U.S. Company Profile contains basic information such as address and telephone number, executive names and titles, line of business and SIC codes, D-U-N-S Numbers, organization status, and year and location of incorporation for Golden West Properties.


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Art News
California Dreaming Artists Best of Show Winners
Thank you to all who attended our California Dreaming artists reception event and to all of our tireless volunteers for ensuring an evening full of fun. Congratulations to our best of show and honorable mention winners!
Art by the Sea Summer Camp - Sign Up Today! Sessions Start July 6!
Our Art by the Sea summer camp is one of the best camps around for teaching children the wonders of fine art. Our teachers are highly qualified. Our prices are incredibly low. We have scholarships available, if needed. Our recently renovated center is conducive to exploring oneself through art.
California Dreaming Exhibit on View Now Through July 20
Thank you to all artists who submitted art and to all of our dedicate, hardworking volunteers for making this exhibit possible. We couldn't have done it without you!
Artists Marketplace First Saturdays 10-4pm in the Arts Center Courtyard
The Carpinteria Arts Center introduces The ARTISTS MARKETPLACE on the first Saturday of each month from 10:00 a.m. to 4:00 p.m. on the grounds of the Arts Center - please check the Events section for upcoming event dates. The Marketplace provides member artists the opportunity to display, demonstrate and sell their original art and to magnetize people and activities to the Arts Center. Please support your local Artists.
Carpinteria Arts Center's "Figure It Out" exhibit. Reception on March 14th, Best of the Best revealed.
"Figure It Out" juried by Valori Fussell. Reception winners revealed on Saturday, March 17 at the Carpinteria Arts Center's artist reception. The exhibit will run until April 20, 2015.
Carpinteria Arts Center
- Foster diverse community involvement
- Nurture the creative process and its appreciation
- Provide educational opportunities
- Establish a Center for the Arts
- Enhance and enrich the quality of life in the Carpinteria Valley
Art Events
Jul 04 Sat
Jul 13 Mon
Jul 22 Wed
Aug 01 Sat
Aug 08 Sat
Aug 29 Sat


text:
Original recipe yields 8 servings
Amount per serving ({{servings}} total)
Based on a 2,000 calorie diet
Ready In
Sooo good! My family loved this too. Next time I think I will use maybe one more avocado. I also used a sweet onion inwith tthe veggies! Delish! Will absolutely make again.
Was very different and good but I should have stayed with the recipe as far as lime juice. The reviews said less but needs it. I also added portobello mushrooms. Needs more broccoli, garlic, and...


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Having an upset stomach is certainly not a comfortable situation, and it's not always possible to visit the doctor for what is seen as a minor and temporary problem. There are many medications at your neighborhood drugstore that promise to offer fast relief, but a natural remedy might actually be the best cure - not to mention the quickest.
Consider Peppermint
One of the most effective options to calm an upset stomach is to use peppermint tea. This is best if you have fresh peppermint leaves. Simply crush the leaves and steep them in hot water for a couple of minutes before drinking the tea. Dried leaves can also be used here.
Bake it Away
Baking soda in water will also help you cure an upset stomach. Add a half teaspoon of baking soda to five ounces of water, and drink it all in one sitting. Baking soda can neutralize excess stomach acid and help calm your grumbling belly.
Embrace the Ginger
Ginger is another natural way to calm your stomach. You can enjoy ginger in numerous ways, from ginger ale to candied ginger, as all work well. This has actually been used in Chinese medicine for several thousands of years to calm down upset stomachs and ease gastrointestinal (GI) problems.
Go Bananas
Finally, you might also consider eating a banana. These are gentle on the digestive system, but can also help to counteract some of the stomach acid that is causing your discomfort. In fact, most physicians recommend bananas as a part of treating children's GI problems.
Bricks4Kidz provides an extraordinary atmosphere for children, where we learn, build, and play with LEGO? bricks. Programs are built around our proprietary model plans designed by engineers and architects, all with exciting themes such as space, construction, and amusement parks. Our specially-designed project kits and theme-based models provide the building blocks for our approach to educational play. We offer activities for children ages 3-13+, including after-school enrichment classes, summer camps, holiday camps, birthday parties, preschool classes, and Kidz Nights Out. Call for more information or stop in today! By appointment only!
We are among the only few companies who can service any automobile, commercial, or residential Locksmith Service, no matter the situation and the time. What makes us better than the rest is our attention to detail and great customer service. We have such a vast selection of keys, locks, security devices, etc. That we can offer them at a lower price than our competition. Not only that, but our rates are based on each job and not how long our technicians are there give us a call today to get more details AT: 703-270-6011. With reliable technicians, it's no wonder we're quickly become the number one Locksmith Company in Ashburn, VA and off the clock. Our emergency service is the best around with 24 hours a day, 7 days a week dependability.
Dental office, ashburn,crowns,veneers,bridges,Cerec ,3d technology , galileos, cosmetic dentistry, fillings, whitening,zoom, late hours, convenient, Spanish speaking
Kumon of Ashburn - Loudoun Station is part of the world's largest after-school math & reading program, helping children of all ages become self-learners.
The material in this article is for informational purposes only. The views expressed in this article are those of the author and do not necessarily reflect the views or opinions of Local.com. See Additional Information
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Original recipe yields 24 servings
Amount per serving ({{servings}} total)
Based on a 2,000 calorie diet
Ready In
I'm glad to see the recipe has been revised! I, too, had to guess at mixing. The size of the pan is also not mentioned. The first time I made them I used a smaller baking sheet, and I had to b...
So easy and fast... If you don't have self-rising flour on hand (which I did not) just be sure to add a little baking soda and baking powder.
I followed this recipe exactly and was quite pleased with the results. Although it's not my favorite bar cookie, it's definitely very good and and I would definitely make it again. I also love...


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At the beginning of the month, Redgate sponsored Talk UX. Revathi and I went to Manchester to attend it, and we've been talking about it to whomever wanted to hear. These are some of the questions we have been asked, here is what you'd have heard us say if you were there too… How was […]
VitaminTalent have created a fantastic infographic that they modestly describe as a 'handy chart, mapping out career options and skill sets for all types of digital creative roles' but one that doesn't take itself too seriously, providing guideline stats for each role – 600 'Arrows drawn hourly' for an Information Architect apparently, although they may have missed a zero off the end…
Related posts
How was Talk UX?By Marine Barbaroux
Release Wednesdays: Deliberate, Frequent ReleasesBy Chris Smith
The team I work with release every Wednesday.
Why? Well, we believe in releasing frequently to give our users valuable enhancements as quickly as possible and to get timely feedback on the direction we are taking the product. We've believe in keeping the delta between releases small and the risk associated with an individual release low. We think practice makes perfect and that the more often you release the less frightening it is to do so.
Moreover, we want to be deliberate about releasing frequently, rather than opportunist. An opportunist frequent release process is one where you are able to deploy a given code change reliably and with little overhead, but that you choose to do this when the opportunity arises. That could be when you've just fixed a bug, when a feature has been completed or perhaps when 'all the planets align' and all your automated tests have finally passed.


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They feel bad, so they found you these other deals to make up for it.
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The Loft Cafe & Social LoungeRead MoreThe Loft Cafe & Courtyard marries the rustic beauty of lodge-like architecture with a modern menu of Pacific Northwest and Mediterranean flavors that change periodically. Their executive chef has crafted a catalog of small and large plates that pair regional meats and seafood with house-made sauces. Large parties can even request a custom menu for their festivities, instead of piling their entrees into the shape of a birthday cake. The chef dispatches all meals to two floors, where shadows cast by hanging lights and black chandeliers play across wooden ceiling grids. He also sends meals out to the courtyard, where towering heat lamps warm hands grasping moscow mules—one of the eatery's many handcrafted cocktails. During happy hour, from 4 p.m. to 6 p.m. and 9 p.m. to 11 p.m. every day, guests take advantage of the lower cocktail prices and while snacking on appetizers from the happy hour menu.515A Main St.Edmonds, WA US
Unexpected ProductionsRead MoreGrab a seat and dig in! Unexpected Productions in Seattle offers tasty eats everyone will enjoy. Tap your foot to Unexpected Productions' tunes — live performances are often showcased here. Weekend diners may find themselves waiting for a table, as Friday and Saturday nights tend to draw a crowd. Whether you have a large or small vehicle, parking is easy near Unexpected Productions.1428 Post AlySeattle, WA US
Capri Ristorante ItalianoRead MoreFaux marbled walls, stark white pillars, and gold- and burgundy-hued drapes create a stately dining backdrop inside the dining room of Capri Ristorante Italiano. An Italian mural is painted in vivid blues and whites on the domed ceiling, and chandeliers illuminate diners sitting in wrought iron and leather chairs, which is the seating style that 17th-century nobility would have envisioned being in spaceships. Notes from a piano float through the air as servers dispense classic Italian dishes such as meat-stuffed raviolis, chicken parmesan, and four-cheese pizza. Outside, a sculpture recessed in the marigold wall watches over al fresco diners, who also get a front-row seat on select nights for the area's summer-concert series.15121 Main St.Mill Creek, WA US
Blazing Onion Burger CompanyRead MoreKick back and enjoy a burger (or two) at Blazing Onion Burger Company. The healthy meal options at Blazing Onion Burger Company are bold, colorful and filled with flavor. You'll find a wonderful selection of drinks from Blazing Onion Burger Company's full bar to top off your meal. No need to splurge on a baby sitter — tots will be right at home chowing down at Blazing Onion Burger Company. Blazing Onion Burger Company is known for its happy hour deals and steals. Get connected at lightning fast speeds with Blazing Onion Burger Company's complimentary wifi. Come order a flavorful feast at Blazing Onion Burger Company, and sit outside if it's nice! The burger joint is quite lively during the week, so seating may be hard to come by. No need to gussy up for a trip to Blazing Onion Burger Company, where patrons dress for comfort and fun. Blazing Onion Burger Company is known for serving great food, and they are able to serve it at your next event with their excellent catering. For those in a hurry, the burger joint lets you take your meal or snack to go. For those who prefer to travel by bike, Blazing Onion Burger Company is a great option due to its generous bike parking options. Leave your piggy bank at home! With prices under $15, you can eat at Blazing Onion Burger Company for next to nothing. Breakfast, lunch, and dinner are all on Blazing Onion Burger Company's menu — you can stop by whenever the moment's right for you.15115 Main StMill Creek, WA US
Azul Restaurant and LoungeRead MoreAzul Restaurant and Lounge takes traditional Mexican fare to the next level. Those looking for a meal that's low in fat won't find it at Azul Restaurant and Lounge, where hearty, tasty meals come first. Complement your meal with a beer or wine from Azul Restaurant and Lounge's delightful drink menu. Little guys and gals will also love dining at Azul Restaurant and Lounge, which offers a family-friendly environment (and menu). Azul Restaurant and Lounge offers discounted prices on food and drinks during happy hour. Enjoy the beautiful weather while you chow down — with outdoor seating, Azul Restaurant and Lounge is a great summer destination. The restaurant can get thronged with crowds on Fridays and Saturdays, so book your table ahead of time through their reservation system. Whether you're coming from work or a ballgame, the dress code at laid-back Azul Restaurant and Lounge is come-as-you-are. Impress the visitors at your next gathering by calling in Azul Restaurant and Lounge for catering. For those in a hurry, the restaurant lets you take your grub to go. If cycling is more your speed, you'll find plenty of space to stash your bike outside the restaurant. An average meal at Azul Restaurant and Lounge will set you back about $30. The dinner menu is a crowd pleaser at the restaurant, though breakfast and lunch are also served.15118 Main StMill Creek, WA US
Ivars Mukilteo Landing Fish BarRead MoreGrab a seat and dig in! Ivars Mukilteo Landing Fish Bar in Mukilteo offers tasty eats everyone will enjoy. Save money on a sitter — kids are welcome to join the table at Ivars Mukilteo Landing Fish Bar. On warmer days, take advantage of Ivars Mukilteo Landing Fish Bar's outdoor seating. If crowds aren't your thing, it's best to visit Ivars Mukilteo Landing Fish Bar during the slower weekday hours. For those in a rush, the restaurant lets you take your food to go. Driving to Ivars Mukilteo Landing Fish Bar? Check out the nearby parking selections and park with ease. Ivars Mukilteo Landing Fish Bar's diners can store their bikes safely at the rack around the corner.710 Front StreetMukilteo, WA US


text: on Mar 22, 2013 @ 6:45 AMMar 22, 2013 @ 6:45 AM PublishedOman's Sultan pardons all jailed activists - state news agencyMUSCAT (Reuters) - Oman's Sultan Qaboos pardoned on Friday all dissidents jailed for defaming the ruler or taking part in protests, in the latest effort to defuse unrest inspired by Arab uprisings elsewhere.The official Oman News Agency did not say how many prisoners would be freed, but activists say courts sentenced at least 50 dissidents to jail terms of up to 18 months last year."His Majesty Sultan Qaboos has issued a royal pardon for those convicted of defamation, information technology crimes and unauthorised rallies," the agency said.Last week a court freed on bail eight activists, part of a group of prisoners who went on hunger strike last month saying they had been sentenced unfairly. The court ordered a re-trial.In recent months the government has sought to ease public discontent by announcing plans to limit the number of foreign workers and sharply raise the minimum wage for locals in a drive to increase employment of Omani citizens.Oman, which sits on the Strait of Hormuz through which some 40 percent of the world's sea-borne oil exports passes, has also pledged to create tens of thousands of public sector jobs.But delays in implementing the promises have kept protests simmering, with some anger focused on the 72-year-old sultan.(Reporting by Saleh Al-Shaibany; Editing by Amena Bakr and Alistair Lyon)


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It has always seemed strange to me . . . the things we admire in men, kindness and generosity, openness, honesty, understanding and feeling, are the concomitants of failure in our system. And those traits we detest, sharpness, greed, acquisitiveness, meanness, egotism and self-interest, are the traits of success. And while men admire the quality of the first they love the produce of the second. John Steinbeck I 'm not sure that I completely agree w … [Read more...]
Attributes of success and failure
"ISIS's rise is related to Islam: The question is: How?"
An important perspective on the so-called "Islamic State" that was published in the Atlantic Monthly back in October. It's still entirely relevant. Perhaps, indeed, even more than it was back then: http://www.theatlantic.com/international/archive/2014/10/the-roots-of-the-islamic-states-appeal/382175/?single_page=true … [Read more...]
Must public employees celebrate gay rights?
Driving home just now, I heard a fair portion of an interview with Eric Moutsos, the former Salt Lake City police officer whose attempt to avoid an assignment connected with last summer's Gay Pride Parade has now reached the national and international news. He was a very articulate and persuasive interview. Based on the little that I've now heard and read about this story, which I haven't previously followed, it seems to me that Salt Lake Police … [Read more...]
"The Milky Way is not as young as it looks"
A nice little article in the 21 February 2015 issue of the Economist is entitled "A galactic vampire": Our galaxy seems to be somewhat more than thirteen billion years old, which makes it not much younger than the universe itself. How does the Milky Way retain its youthful good looks? Why, the same way that I do: It sucks the lifeblood of others! The article is a quick read. For some reason, though, the direct link to it has proven unstable. … [Read more...]
This is an absolutely fabulous, heart-warming story
May God bless all who were involved: http://abcnews.go.com/Health/showed-autistic-boys-birthday-party-strangers-saved-day/story?id=29166772 … [Read more...]
"Fifty Shades of Dark. And One Shade of Feminism."
I just got off the phone with a journalism student at Loyola University in Chicago, who wanted to interview me for an article that he's writing on the topic of "Ordain Women." I had assumed that he was writing for the student paper there, but he indicated to me that he's hoping for wider publication. It was a pleasant interview, although, based upon my experience with such encounters, I wouldn't be surprised if I either don't show up in the final article at … [Read more...]
New Testament 86-88
Matthew 8:14-17Mark 1:29-34Luke 4:38-41; 7:11-17 Only Luke recounts the story of Jesus raising the son of the widow of Nain from death. But all three of the synoptic gospels mention the healing of Peter's mother-in-law (πενθερά). It's striking that the first pope, as he's often regarded, had a mother-in-law. That fact rather suggests, unless I'm missing something, that he was married. The traditional site of the … [Read more...]
"Prophets and presidents: 11 noteworthy connections between the LDS Church and the White House"
For those who may have missed this piece: http://www.deseretnews.com/article/865622644/Prophets-and-presidents-11-noteworthy-connections-between-the-LDS-Church-and-the-White-House.html?pg=all It's really quite interesting, and provides several surprising "Gee whiz!" moments. … [Read more...]


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VIEWS: 6 PAGES: 3 POSTED ON: 6/8/2012 Public Domain
FIRELOC Introducing ACW's "FIRELOC" line of heat resistant plastic buckles, slides, hooks, etc. When you are in the heat of a situation, you don't want your personal protection equipment to melt off. ACW Offers a complete line of "Thermal Resistance Nylon" Plastic Hardware ACW has released a new 2" side release buckle designed primarily for high heat applications. This buckle has been tested and approved by Underwriters Laboratories for use in the United States and Canada. The ACW S/#'s, item descriptions and UL/ULC certified safety standards are listed below: Stock Number Pattern Color Width Material 91988 MSR Black 2" Heat Stabilized Nylon – Male Only* 91990 MSR Black 2" Heat Stabilized Nylon – Female Only* UL/ULC Certified Safety Standards • NFPA 1971, Standard on Protective Ensembles for Structural Fire Fighting and Proximity Fire Fighting, 2007 Edition • NFPA 1951, Standard on Protectice Ensembles for Technical Rescue Incidents, 2007 • NFPA 1977, Standard on Protectice Clothing and Equipment for Wildland Fire Fighting, 2005 Edition • NFPA 2112, Standard on Flame-Resistant Garments for Industrial Personnal Against Flash Fire, 2007 Edition • NFPA 1975, Standard on Station/Work Uniforms for Emergency Services, 2009 Edition • NFPA 1999, Standard on Protective Clothing for Emergency Medical Operations, 2008 Edition • CAN/CGSB-155.1, Standard on Firefighters' Protective Clothing for Protection Against Heat and Flame, 2001 Edition • CAN/CGSB-155.20, Workwear for Protection Against Hydrocarbon Flash Fire, 2000 Edition • CAN/CGSB-155.22, Fireline Workwear for Forest Firefighters, 1997 Edition WEBBING ACW also manufactures a line of webbing products utilizing specialty yarns, including Kevlar, Nomex, PBI and Dyneema. For information about ACW FIRELOC Products, please contact us at (401) 762-5500 or visit us at www.acw1.com. American Cord & Webbing Co., Inc. 88 Century Drive, Woonsocket, RI 02895-6161 • Ph (401) 762-5500 • Fx (401) 762-5514 American Cord & Webbing Co., Inc. ("ACW") does not assume responsibility for defects, damages or injuries caused by misuse, abuse, repair by others or unsuitability as to applications or load bearing strength. Notwithstanding anything to the contrary contained in any information or promotional materials furnished by ACW, all sales of ACW are governed exclusively by its standard terms and conditions which include disclaimers of liability. tm tm
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Regular Price: €17.75Your Price: €16.10You Save: €1.65 (9%)
A MASSIVE assortment of 72 flowers from Prima. This large jar is jam-packed full of flowers in all shapes, sizes and colours.
A must have for any flower fans!
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Cardz'n'Scrapz is a service of Cork Art Supplies Limited


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Brickell Biotech, Inc. is a clinical-stage pharmaceutical company focused on the development of innovative and differentiated therapeutics for the treatment of skin diseases. Our current pipeline consists of new molecular entities targeting the treatment of the following indications: hyperhidrosis, atopic dermatitis, contact dermatitis and acne.
Brickell Signs Exclusive License and Development Agreement for its novel, topical soft-anticholigenric, BBI-4000, with Kaken Pharmaceutical Co., Ltd. in Japan.


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USMNT Struggles Continue As They Lose Late To Denmark
What The USMNT Must Do Moving Forward To Get Out Of Their Slump The slump continues for USMNT as they let a late one goal lead slip again in a 3-2 loss to Denmark on Wednesday night. The US
March 13-15 MLS Predictions
MLS Score Predictions The second week of MLS is coming up, and after an exciting first week, here are my predictions for how this weekend will turn out. Orlando City SC at Houston Dynamo (2-1 Houston) Houston was extremely
2015 Preseason Expectations: Toronto FC
Toronto FC 2015 Predictions Before the official 2015 MLS season begins, I'll be making predictions for every team; here's Toronto FC. 2014 Record: 11 Wins; 15 Losses; 8 Ties Key Off-Season Moves: CDM Marco Delgado (Dispersal Draft); F Robbie
USA Men's Soccer Team Gets First Win of 2015
Dempsey and Bradley Both Score For USA The USA Men's National Soccer Team (USMNT) got their first victory of 2015 by beating Panama 2-0 and two big stars scored two amazing goals for the win. Michael Bradley had the
Video: Michael Bradley Epic Goal vs. Russia
Michael Bradley just scored what was quite possibly the goal of the week with a volley from the top of the box


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Product Reviews
Not Marbled Glass
Well, we were expecting the glass to be marbled as the description states and smooth, but be aware that the glass is NOT smooth, it actually has full size leaves pasted onto the glass, as if the glass was showcasing fossilized leaves. Sorry if this description is not clear. The light is still pretty and the leaves give it a natural look, however if you were expecting a smooth "marbled" look, you will be dissappointed. This is a nice light if you can live with the leaves. The description really fails to describe this product properly.
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* TECHNICS GN 9 * Learning to play has never been easier or more FUN .! Whatever your age.... you are never too young, or too old to learn.......its EASY ! MODEL ....................... TECHNICS sx-GN 9 just a few years ago these babies were the "TOP OF THE LINE " ..................... Recommended Retail Price was a staggering $20,000 + ( no mistake ) Just like having a full ...
Jun 10 - Saint Cloud - Musical instruments
What do you have to trade of equal value? We have two sets of double wide classroom trailers each 24 x 36 wide. One has been extensively repaired (around $2000 in repairs - materials/labor) over the past couple of weeks. One trailer is priced to sell at $2500 and the repaired trailer is priced to sell at $2000. Buy the pair and set them up together to make a quadruple wide office, hunting ...
May 31 - Eustis - Business & industrial
Six Beautifully Shaded Cemetery lots located at Highlands Memorial Park Cemetery, Ocala, FL Lot 32, Section H, 2 lots/$2,600 Lot 30, Section H, 4 lots/$5,200 OR can buy all 6 lots together located side by side for $7,200.
Jul 3 - Gainesville : Alachua - Everything else
Two Cemetery Lots in Woodlawn Memorial Park (Section E, Masonic Section) in Gotha/Orlando, FL. Sold out Section. Valued at more than $2500ea. Asking $3200/OBO
Jul 2 - Gainesville : Bell - Everything else
This is a wooden Responsibility Chart for children 4 years old and up. There are two sections hinged together and a cord allowing it to be hung up. Each section measures 15-1/2" wide x 11-1/2" high x 1/4" thick. The top section is a grid with a column for Goal, Sunday, Monday, Tuesday, Wednesday, Thursday, Friday, Saturday, Result The bottom section is a blank dry erase board. A dry ...
Jun 29 - Daytona : Daytona Beach - Baby & kid stuff
Jun 22 - Tampa Bay : Palm Harbor - Business & industrial
The Franklin Library, 1980. first limited edition. octavo in burgundy leatherette with gold gilt and letters; embossed spine with five bands; VG+; notes pamphlet from the editors included; in good condition; as new; burgundy sewn-in bookmark and flaps; pp. 186; shelved in Drama section.
Jun 21 - Cape Coral : Fort Myers - Books & magazines
Paris: Lemarchand, 1802. new edition;. 12mos. in light brown leather; Good; 5-band spines with gold letters and burgundy frame; some amount of shelf wear mostly on spine and edges; binding strong; previous owner's tiny seal on title page; frontispiece portrait loose; paper faded and foxed, text clean in tight print; in FRENCH; shelved in France section.
Jun 21 - Tallahassee : Tallahassee - Books & magazines
Spaces 1-2-3-4, Lot 166-D, Garden of The Tower,Section 15, Unit No, Two. In Garden of Memories, Tampa, Fl. Minum sale of two lots Spaces (1&2) 0r (3&4) or all four spaces. The price is each space. Cost of all four lots can be negotiated.
Jun 20 - Tampa Bay : Tampa - Everything else
Cablofil wire mesh cable trays, 10' x 18" x 6", electrozinc plated steel sections. Mod# CF150/450EZ. UNSPSC #39121204. Capacity 78.85 Lbs Per 6 Ft Section. Graingers has this listed for $350 per section. Save by buying these slightly used sections in excellent condition! $50.00 each section Item# 663 Located at 34219, in Northeast Manatee County, about 40 miles southeast of Tampa. Call 941 ...
Jun 18 - Sarasota : Parrish - Business & industrial
Six months old, moving and sofa is too large to take. Three pieces black bonded leather. Very comfortable, tufted seat cushions, grid-like accent stitching and wedge feet. Back cushions are attached and seat cushions are velcro-adhered. Sharp and still like new! Will text photos to you.
Jun 17 - Lakeland : Lakeland - Everything else
A pair of tickets for the Sam Smith concert July 20th. Includes parking pass. Section 115, Row 21. Paypal only.
Jun 10 - Miami : Miami - Tickets
Fri 06/12/15 7:30 PM THE AVETT BROTHERS & JOHN PRINE AT MEADOWBROOK Bank of New Hampshire Pavilion Reserved Seating (Covered Pavilion Section 2B X 2 tickets Kubota Ultimate Tailgate Parking Pass
Jun 9 - Palm Bay : Melbourne - Tickets
Family Double Depth Cemetery Plot for Sale. Site 1, Lot 221, Section 26C. Two vaults included as well as a memorial marker. Located at Woodlawn Memorial Park South 11655 S.W. 117th Avenue, Miami, Florida. Asking $6200. Owner will pay transfer fee. This is a private sale and not brokered. Available for immediate need. Woodlawn Park Cemetery South was established in 1967 and is considered to ...
Jun 9 - Miami : Miami - Everything else
6 inch sexy stiletto heels perfect for going out in or some fun bedroom play HURRY won't last long at this price !! I accept paypal i can also ship anywhere in the us for only $5 All these heels are sizes 6.5
Jun 8 - Daytona : Palm Coast - Clothing & accessories
Schiess vbm cnc 177 inch 196 in swing 98 in turn ht 2008 Location: Europe - All Ad Status: New! Manufacturer: Schiess Type: vbm cnc vertical boring mill cnc Fan OI never in p Model: 177 in 196in 98 in turn ht Year: 2008 Condition: excellent Price: - USD Quantity: 1 Type of Seller: Dealer Non-Owner Description: (Schiess Type) 177" CNC Double Column Vertical Turret ...
Jun 8 - Tampa Bay : Palm Harbor - Business & industrial
Ingersoll MASTERCENTER 5 FACE 98X 78Y 39Z FAN15MB 11 PALLET Location: U.S.A. Ad Status: New! Manufacturer: Ingersoll Type: MASTERCENTER 5 FACE Model: INGERSOLL MASTERCENTER 5 FAC Year: 1996 Condition: Very Good Price: - USD Quantity: 1 Description: (3-Machines) 1996 Model: Master Center Ingersoll 5-Face CNC Machining Center FANUC 15MB 5-Axis CNC Control TRAVELS: X ...
Jun 6 - Tampa Bay : Palm Harbor - Business & industrial
Two burial plots side by side in HOLLYWOOD MEMORIAL GARDENS CEMETERY in desirable Gardens of Memories Section, Lot 406, Spaces 1 and 2. These were purchased for family members who are no longer residing in the South Florida area and are interested in selling. Price is very negotiable. Current retail price is $3,995 per space. All arrangements will be handled by the seller. Cemetery location ...
Jun 6 - Miami : Hollywood - Everything else
2015 UEFA Champions League Final Tickets (Juventus vs FC Barcelona) Order your CHAMPIONS LEAGUE FINAL 2015 ticket today and be part of the live action at Olympiastadion, Berlin, Germany Time:8:45 PM. (20:45) Saturday, June 6 2015, Olympiastadion, Berlin, Germany Number of tickets available for sale:3500 Please select category ,and number of tickets:VIP Gold - Good Alongside Tickets. CAT ...
Jun 2 - Miami : Palm Beach Gardens - Tickets
A Pair of Sam Smith tickets for a sold out GA Floor section. They are ticketmaster ticketfast print at home tickets. Willing to meet up around NE Miami Dade or SE Broward. Or a PayPal transaction can also be arranged if necessary.
Jun 1 - Miami : Miami - Tickets
60" Chuck 92" Swing over way * lathe sits on 20' section and there are 3 more sections at 20' a piece total 80' long 50 hp. motor 2 Steady rest Taper Rapids Large amount of tooling and boring bars INV190412 Please see our store below for other Lathes
May 28 - Tampa Bay : Palm Harbor - Business & industrial
hitachi zosen 6000 ton 11000mm LR x 2000 mm FB Location: Europe - All Ad Status: New! Manufacturer: hitachi zosen Type: Single Action Straight Side 2-Point Mechanical Pre Model: s2 6000-1100-200 6000 ton Year: 1983 Condition: excellent Price: - USD Quantity: 2 Type of Seller: Dealer Non-Owner Description: (2) 6,000 ton Hitachi Zosen (USI Clearing) Model S2-6000-1100-200 Heavy Duty Long Bed ...
May 25 - Tampa Bay : Palm Harbor - Business & industrial
THEY BREAK DOWN FOR EASY TRANSPORT AND SET-UP. THE LARGE HOG TRAP will break down into three sections are 8 FT 2 INCH SMALLER HOG TRAPS ( 8 FT long X 35- 36 inches wide X 36-37 inches tall ) . PRICE $ 250.00 . THE SMALLER HOG TRAP comes apart TWO PIECES -(8 FT LONG - 47 INCHES WIDE & 35 INCHES TALL PRICE $ 225.00 I USUALLY HUNT ALONE, SO THEY ARE BUILT FOR ONE MAN TO HANDLE, EASY HOG ...
May 22 - Augusta : Tarrytown - Farm equipment
You may reach me by email, but leave your phone number on it. I do not reply by email. No text messages please, I don't get them. 904 576 1750 There are three steps to this aerobic stepper. This is hard to measure because of the curved edges, but all together, it is about 8 1/2" tall, just short of 26" long and 16 3/4" wide. There are scuffs on it, and they might come off, but I have not ...
May 21 - Jacksonville : Jacksonville - Health & beauty