text:
Jump to content
Myer Bondi Junction removes plus size clothing section
Started by melandned , Jan 07 2013 03:16 PM
45 replies to this topic
Posted 07 January 2013 - 03:16 PM
How does this make you feel? I am so angry - I am right on the cusp being a size 16 so don't necessarily need the section but I feel it's totally discriminatory.
My Size was gone the last time i went too
Are they not getting the customers there or do they not want the plus size customers?
Posted 07 January 2013 - 03:20 PM
Maybe ask?
They might come back with differend (plus size) line or simply don't havevthe clientele for it?
I'm a 16/18 but can't really see this asva reason to get angry as such. One less customer for them!
Posted 07 January 2013 - 03:21 PM
Retail is really hard at the moment - look at the sales. If they were making money from it they wouldn't remove it.
Posted 07 January 2013 - 03:26 PM
i did ask, they lady didn't know just said you have to go to the city now to purchase
Posted 07 January 2013 - 03:26 PM
Maybe it isn't a Bondi thing to be over a 14 because the plus size section at our local Myer store is taking up more floor space and increasing the number of labels.
It must be more acceptable to be cuddly in Melbourne's eastern suburbs
Is there another you can try close by OP?
Posted 07 January 2013 - 03:26 PM
You aren't going to get rid of something that was turning a profit. It obviously wasn't.
Shops aren't community services - they are money making machines.
Posted 07 January 2013 - 03:26 PM
Appalling, but pretty in line with anything I have seen happen in the East. I don't identify with the culture over there in the slightest and size snobbery is rife. I worked over there for many years, in and around fashion people.
I'd be happy if I never went East again - prejudiced... yes, I admit it.
Posted 07 January 2013 - 03:29 PM
QUOTE (countrymel @ 07/01/2013, 03:26 PM) <{POST_SNAPBACK}>
You aren't going to get rid of something that was turning a profit. It obviously wasn't.
Shops aren't community services - they are money making machines.
In Bondi, it probably wasn't suiting the majority of their clientele, hence they got rid of it to expand the sections/departments that are making them some money.
Posted 07 January 2013 - 03:30 PM
Not sure how it's discriminatory. It was clearly not a money earner for them so they removed it.
Posted 07 January 2013 - 03:32 PM
ok fair point
Posted 07 January 2013 - 03:43 PM
IMHO the choice of clothing for sizes 16+ in Australia is mostly hideous, and I think customers are turning to internet shopping from the US and UK - the prices are far cheaper and the options are many and varied. Although I do like Taking Shape clothes, they are waaaaay expensive.
www.hipsandcurves.com (for lingerie)
www.asos.com (plus size range called Curve) free shipping to Australia
www.newlook.com (plus size range called Inspire)
to name a few...
Posted 07 January 2013 - 03:48 PM
I don't see it as being discrimination.... obviously it just wasn't paying off. The demand for plus size clothing in that particular Myer store is probably not really there.
Posted 07 January 2013 - 04:31 PM
Miranda Myer has 1/2 a floor of plus size labels. And some half decent styles too.
Personally I hate shopping at Westfield Bondi. The stores are all too trendy for me and way too spread out . And now that it has Zara I'll be avoiding it like the plague.
Posted 07 January 2013 - 04:35 PM
I'm in the plus size range and never ever shop at myer for clothes. They ate over priced and for an older demographic. I buy all my clothes from UK & US stores as the clothes are in fashion and aren't feral.
We in Australia have the worst range for plus size. Good thing I'm going to the UK soon and hopefully 15 kilos lighter get my shopping on.
I don't think it's discrimination they are saving the people of bondi from the feral myer plus size fashions that the "designers" think are loved.
Posted 07 January 2013 - 04:40 PM
how very frustrating, David Jones in Chadstone here in Victoria doesn't stock plus size, but David Jones has never been great for the bigger girl. Our Myer has a growing section.
Posted 07 January 2013 - 05:08 PM
Yeah why should fat people expect to be ale to go to a shopping centre like everyone else and buy clothes, they are sub human anyway so their needs don't matter.
Posted 07 January 2013 - 05:24 PM
QUOTE (Orangedrops @ 07/01/2013, 06:08 PM) <{POST_SNAPBACK}>
Yeah why should fat people expect to be ale to go to a shopping centre like everyone else and buy clothes, they are sub human anyway so their needs don't matter.
I agree with this level of sarcasm. I think Myer Bondi should stock Plus Size clothing. I don't understand all the leniency here, but hey, we're all entitled to what we think
Posted 07 January 2013 - 05:26 PM
Fat people aren't allowed in the Eastern Suburbs of Sydney - didn't you get the memo?
Posted 07 January 2013 - 05:27 PM
I am plus sized and I too like the option of a good browse around the shops.
Given that more than 50% of the women in Australia are over a size 14 there is a large plus size market - it's such a shame that Australian retailers think it's ok to not stock a good plus size range - this just pushes the online option which whilst good for the shopper isn't great for the Australian retail/clothing manufacturer and as an Australian plus size women's clothing manufacturer I can tell you it's bloody hard work getting a foot in and showing women that there are options for them.
My money is just as good as a lady who is a size 12 but alas Westfield don't see it that way - pushing out small independent boutiques are charging MASSIVE rents meaning every square cm of a shop must produce big $$$$$ if it wants to stay on the retail floor. Feel free to PM me if you'd like to know about my brand which fills a niche in the plus size womens clothing market.
Edited by fooiesmum, 07 January 2013 - 05:57 PM.
Posted 07 January 2013 - 05:30 PM
You know those little people that you see at Luna Park that say "You must be this tall to ride here"? Well, at the bottom of Oxford Street they have a cut out silhouette of a model. Underneath is written "you must be this thin to enter here". Apparently that's how they keep fat people out of the Eastern Suburbs.
Posted 07 January 2013 - 06:25 PM
Obviously the demand is not there. They wouldn't take something out if it was selling well. It's not a charity.
Shopping for clothes is a privilege not a right, I don't get the out-rage... simply go to another store.
Edited by LambChop, 07 January 2013 - 06:25 PM.
Posted 07 January 2013 - 07:19 PM
Sorrier for the desperates in the eastern suburbs who have to stay lollipop size all the time or be dumped by their sugar daddies or bosses or size 6 "friends" who wouldn't want to be seen with a fat chick.
Give me the country anytime
Posted 07 January 2013 - 07:54 PM
Edited by sparkler, 20 January 2013 - 11:35 PM.
Posted 07 January 2013 - 08:10 PM
There are certainly women size 14 and over in the Eastern suburbs, I know a lot of them! The thing is being the Eastern suburbs the range at Myer isn't their idea of fashion! More likely to be seen in flowing Camilla caftans than 3/4 pants with elastic waistbands or under the bust flared dresses!
It's supply and demand, Myer isn't exactly cutting edge in their plus size, the women of this area can afford to pay more and go elsewhere.
Saying all Eastern suburb women are size 6 sugar Daddy enabled women is insulting and untrue. You'll find the majority have a career and earn equal to their husbands...... Not sugar Daddy!
Sounds like touch of jealousy from some EB women....
Posted 07 January 2013 - 08:18 PM
Of course there are plus sized people in Bondi, they just don't shop at Myer.
Probably pick up items on overseas trips.
1 user(s) are reading this topic
0 members, 1 guests, 0 anonymous users
Get your FREE Baby & Toddler Show ticket!
Get your free ticket to the Sydney Essential Baby & Toddler Show for September 25-27 - register online now.
Win Love Child Season 1 & 2 on DVD
To celebrate the release of Love Child Season 2 on DVD from July 9, Essential Baby and Universal Sony Pictures Home Entertainment are giving away Love Child Season 1 & 2 on DVD to 13 lucky winners.
A solo birth, a wasp swarm and a forest fire: mum and baby's amazing story of survival
Desperate, out of petrol and low on food, a new mother lit a fire in the hope of attracting attention.
Boy found on swing died of hypothermia and dehydration, autopsy finds
The story was chilling and heartbreaking: a three-year-old boy was found dead in a Southern Maryland park, his mother pushing him on the swing.
Child's play and laughter help battle fatigue
Feeling fatigued? Uh-huh, thought as much. Join the queue.
Dad shares entertaining 'how to hold a baby' clip
For many new dads, their own child is the first baby they have ever held. So one dad has posted an instructive YouTube video titled "How to Hold a Baby".
The Australian baby with 100,000 Facebook fans
She may be only eight months old, but Egypt has already amassed more than 100,000 fans and received a letter from royalty - Hollywood royalty that is.
Public welcome outside church for Princess Charlotte's christening
Duke and Duchess of Cambridge have invited well-wishers to see Princess Charlotte outside church in Sandringham on day of her baptism.
Tongue tie: what you need to know
Tongue and lip tie can lead to many problems for babies - and their parents. Here are the signs of tongue tie and how it's treated.
My daughter is small but that doesn't matter
My daughter may be small, but it's my job as her parent to refocus back where it belongs - on who she is as a person
Wet wipes linked to rise in allergic reactions
The government has issued a health warning after a rise in allergic skin reactions has been linked to a preservative found in some wet wipes.
Gay couple in their 80s first to wed in Dallas after Supreme Court ruling
Love may have won, but it came with quite the wait.
William Tyrrell's family marks birthday with cake and renewed appeal
The family of missing boy William Tyrrell will mark his fourth birthday on Friday making a cake to share with friends and family as NSW police renewed their public appeal for information on his disappearance.
What all parents should know about safe babywearing
A picture of Ryan Reynolds always gets the girls talking, and a recently shared photo has done exactly that - but this time, it's for all the wrong reasons.
Baby's head shape reveals potentially fatal condition
Thinking her baby just had an unusually shaped head, a mother was shocked to discover it was instead linked to a dangerous condition.
'Help - my toddler hits me!'
My toddler has started hitting when he gets frustrated, is feeling ignored, or just thinks it might be fun.
Why IVF success rates may not be what you think
Transparency, accountability and responsibilityare essential measures to protect IVF vulnerable patients.
On the 10th anniversary of my son's death
This day marks a significant day. Today marks 10 years since I lost my son Kai.
Mother-in-law 'from hell' inspires survival guide
The happily ever after Nicola Milan had imagined wasn't to be – and she blames her mother-in-law.
Name your baby Quinoa, win a $10K gift card
Choosing a name for your little bundle of joy is always a major decision. It can be something traditional, trendy, creative … or inspired by the menu of your favourite chain restaurant.
Owning a pair of nail scissors does not make me a hairdresser
It's been a whole year since sleeping in until 10am. A whole year since having a peaceful shower.
The 83 children who were tragically let down in the last decade
Over a 10-year period, 83 children died from domestic violence abuse in NSW, with three quarters of the victims aged five years or under, the NSW Ombudsman has revealed.
Expert Q&A: Gross motor skill development in toddlers and preschoolers
Dr Katie Heathershaw answers questions about jumping, toe walking, riding a bike and being pigeon toed.
Is it reasonable to expect your partner to give up drinking in pregnancy?
From the moment that I fell pregnant with my son, I realised just how much my life had already started to change.
Stroke victim joins class action against makers of popular contraceptive pill
"I was terrified I would always be this way. The pill needs to come with a much higher warning."
Sexy time
Why you should get excited about scheduling sex
Unfortunately, the belief that sex should always be spontaneous is a myth. It just isn't.
When newborn photoshoots get messy
When it comes to newborn photoshoots, it is all about the timing.
Orphaned baby daughter Ayla wakes from coma
Former All Black Jerry Collins' critically injured orphaned daughter has awoken from her coma and is able to bottle-feed.
Dad takes miraculous catch while feeding baby
One American father has taken multitasking to a new level at a Cubs-Dodgers baseball game at Wrigley Field.
'Samuel is our firstborn, and he will never be forgotten'
Having lost their firstborn at one day old, the Carrolls were overjoyed to welcome their daughter Isobel into the world a year later.
Channel 10's Sarah Harris expecting first child
The Studio host Sarah Harris doesn't mind if her first baby is a boy or girl, but she does hope it is born with one thing in particular.
Top 5 Articles
Skip to:
Recipe ideas
We are discussing...
What's hot on EB
27 funny ultrasound pictures
Ultrasounds give you a look at your growing baby ... and sometimes what appears to their womb-buddy, or your bub in an amusing position.
The top 6 misleading parenting terms
From 'morning sickness' to 'the terrible twos', there are many parenting terms that are misleading.
When 'good' nannies go bad
While most nannies take pride in their work, there can be some who have a hidden side.
Woman hospitalised for skinny jeans injury
Beware: skinny jeans might be bad for your health.
Gauze seeding: the bacteria-breeding birth trend
A number of women having caesarean deliveries are now taking steps to give their baby a better 'microbiome' start in life.
Jimmy Fallon writes new children's book for dads
Jimmy Fallon, host of NBC's The Tonight Show, recently wrote a children's book about every father's secret wish for their baby's first word to be "dada" - not "mama".
28 names for babies born in winter
Looking for some baby name inspiration for a bub born during the colder months? Here are 28 options from around the world to consider.
The horrible act that sparked a brawl at child's birthday party
The uncle of the seven-year-old girl at the centre of the brawl at child's birthday party in Sydney's west has described the events leading up to the alarming show of violence.
Babies 'benefit from iPads at a young age': study
More often than not, you'll read that screen time for children should be kept to a minimum - but some scientists are now challenging this way of thinking.
Do mums really just obsessively talk about their children?
Natalie Reilly describes three main types of conversations mothers have. And, surprise, they're not all about kids.
Why some dogs might attack babies or young kids
A baby's smell, the noises it makes and even its gaze can contribute to the potential for a dog attack.
Mum demands refund for 'beargina' christening cake
It was meant to be a tasteful cake to help celebrate a three-year-old's christening.
5 things no one warns you about after giving birth
How many times have you been warned about all the sleepless nights you have to 'look forward to' when you become a parent?
Police officer sang nursery rhyme as heartbreaking photo was taken
A police officer arrived at a devastating scene on Thursday: a car crash resulting in all passengers being thrown from the vehicle.
Don't worry, working mums: Just leave Dad in charge at home
Want to open the boardroom doors for women? Encourage - heck, praise - dads who stay home with their children.
Hilaria Baldwin shares post-baby selfie
Just two days after giving birth, actor Alec Balwin's wife posted a post-baby picture on social media.
'Help - my child won't ever do what I ask!'
Compliance is part of the parent-child relationship, but so is resistance. It's all natural.
Postnatal depression support gets $23 million boost in NSW
The Baird government will include $22.8 million in Tuesday's NSW budget to expand a program designed to help parents at risk of postnatal depression (PND).
'I'm just as tired, scared and stressed as you': stay-at-home dad's plea
I'm really lucky to have two great kids, but I found it really tough with so much being aimed at the mothers and not the fathers.
Get your FREE ticket to the Baby & Toddler Show
Get your free ticket to the Sydney Essential Baby & Toddler Show for September 25-27 - register online now.


text:
INDIANAPOLIS — Excitement filled the air when an Indianapolis church received its nonprofit designation from the Internal Revenue Service this week.
The Indianapolis First Church of Cannabis was officially named a 501(c)(3) charitable organization, pushing forward the religion that promotes marijuana use.
COLUMBIA CITY — The Whitley County Historical Museum is bringing the War Between the States back to life again as it hosts its seventh annual Civil War Camp from June 9 to 11.
The camp is open to students ages 10-13. The children will learn firsthand what it was like to live in the U.S. during the 1860s. Campers will be enlisted as either Union or Confederate soldiers, while learning the history of each side.
Columbia City High School held its annual girls track banquet last week. Coach Bob Fahl presented the following awards: (Front row, from left) junior Hannah Wappes, Most Valuable Runner; freshman Alissa Jagger, Most Improved Runner; senior Kailynn Miller, Most Valuable Track Athlete; senior Lauren Ottinger, Most Improved Field Event; (Back row) senior Samina Qureshi, Coaches Award; senior Morgan Von Seggern, Mental Attitude; senior Sydney Burdge, Most Valuable Field Event; and senior Abbi Gottfried, Rookie of the Year.
COLUMBIA CITY — As an ensemble of the Fort Wayne Children's Choir (FWCC), the Whitley Community Children's Choir (WCCC) just finished its fourth year of performances and musical education.
The group performed for Whitley County Holiday Pops, Santa Parade, Christmas at the Courthouse, Fall Festival, nursing homes and the Farmer's Market this past year.
COLUMBIA CITY — In the past, every student in the area had to be in the same classroom, regardless of their age. The world of education has changed and not every student completes school in the same fashion.
This is where T.R.O.Y. Center is helpful. T.R.O.Y. Center, Whitley County's alternative school, specializes in finding success for students who have difficulty in the regular classroom.
COLUMBIA CITY — Columbia City High School junior first baseman Carson Reed earned a place on the All-Northeast Hoosier Conference second team at the corner infield position.
Reed compiled a .414 batting average while pounding out 29 hits. Reed hit three doubles, two triples and one home run during the just-completed season.
Columbia City's baseball team finish the 2015 season with a 6-17 record, bowing out in sectional competition with a loss to Bellmont.
SOUTH WHITLEY — Whitko High School recently held its Underclassmen Academic Awards. Jasmine Gregory and Maddie Shultz were awarded for three years of outstanding attendance. Juniors Nicholas Crosson, Nichole Crouch, Jacob Hyser, Veronica Palmer and Nathan Wiehe as well as sophomores Ronald Brown, Brandon Oaks and Emily Riddle received awards for two years of outstanding attendance.
Whitko's 627 Awards were given to Casey Dierks, Mikayla Findley, Erick Loe, Morgan Miller, Veronica Palmer, Garrett Smiley and Cecilia White.
BUTLER — First came a conference tournament championship. Then came a Northeast Corner Conference regular-season championship. Monday night, Churubusco's Eagle baseball team added an IHSAA sectional title with a nail-biting 6-4 win over the Bluffton Tigers in the title game of the Eastside 2A Sectional.
As they have throughout this year's sectional, the Eagles used a big inning to build a lead. However, Monday evening, the Eagles had to hang on against Bluffton.
SOUTH WHITLEY — Churubusco High School announced scholarship winners from the class of 2015 Friday in the high school auditorium. Numerous local individuals and organizations offered financial assistance for graduates pursuing further education.
Awarded in the Gaylord Jackson Top Five were Valedictorian Valerie Pope, Salutatorian Peyton Brandt, Kennedy Sade, Devon Zeigler and Madison Bieber. Each of the top five received a $700 scholarship.
COLUMBIA CITY — With a wide variety of academic levels in the district, Whitley County Consolidated Schools worked hard this school year to meet the needs of all students.
WCCS has a handful of students who have been labeled "high-ability" and about 18 percent of its enrollment is "special education."

subset: commoncrawl


text:
FiltersShow / Hide
- All Events
- Music
- Comedy
- Theatre & Shows
- Sport
- Cinema
- Festivals
- Days Out
- Edinburgh Fringe
- Anywhere In The UK
- Location: Anywhere in the UK
Radius: ... miles
Situated amidst magnificent park and woodland, 4-star visitor attraction Callendar House tells stories spanning 600 years of Scottish history. Through wars, rebellions and the industrial revolution, the house has played host to many great historical figures over the centuries, including Mary, Queen of Scots, Cromwell and Bonnie Prince Charlie. See history brought to life by costumed interpreters in the working Georgian kitchen, or find out more about the House's colourful history from the permanent displays. With a changing programme of temporary exhibitions, archives, gift shop, The Park Gallery and Teashop at the Stables, a day out at Callendar House offers something for everyone.
Callendar House currently has no upcoming events
Track Callendar House
- You are tracking Callendar House
- Email alerts will be sent to your email address
- Be the first to hear about new events at Callendar House
- Alerts are free & always will be
- 1,146,269 fans now rely on Ents24 alerts
- We hate spam, your email will never be shared
We do not currently have any events for Callendar House
Track Callendar House be the first to know about new tour dates
Tracking Callendar House you will be the first to know about new tour dates
Email me when new Callendar House events are announced.
You are signed up to Callendar House events. We will let you know as soon as new dates are announced.
- Receive email as soon as new events are listed
- 1,146,269 fans now rely on alerts to follow their favourite artists and venues
- Alerts are free and you can unsubscribe at any time
- We won't pass your email address to anyone else
Venue Facts
Venue: Callendar House
Callendar Park
Website: falkirk.gov.uk/services/community/cultural_services/museums/museum_venues/callendar_house.aspx
Enquiries: 01324503770
→ Falkirk Grahamston 17 min walk
News and Announcements RSS feed
No current news or announcements.
Have Your Say
- Have you been to Callendar House? Why not write a review?
Do you represent Callendar House, or are you a member of their promotional team? Click here to claim this page


text:
Starting the Conversation about Mental Health in the Profession
Voicing the concerns of Ontario's lawyers and the public at the provincial, national and international levels.
Join the Canadian Bar Association (CBA) and benefit from the numerous services offered by the Ontario Branch (OBA).
If you missed these hot programs from Institute 2015 – this is your chance to catch up.
Get more from your CBA-OBA membership
A complimentary service for lawyers and legal professionals.
Search for an OBA member lawyer based on name, firm, location, area of practice and/or language spoken.
Join the Re-Think Virtual Community and shape how the CBA will serve you in the future.
Join today and July and August will be included in your annual membership year!
DEVOTED TO JUSTICE: Brian King's 20-year pursuit of a killer. And much more - Spring 2015 issue.
Customized support, strategies and opportunities to talk more about mental health within Ontario's legal community
Looking for a lawyer?
Search for an OBA member lawyer based on name, firm, location, area of practice and/or language spoken.


text:
Easter Clip Art page 2
Featuring Mickey Mouse, Pluto, Goofy, Donald Duck, Daisy Duck, Pooh Bear, Piglet, Eeyore, Rabbit, Tigger, Kanga and Roo
Last updated on July 1st 2006. Click on images for full size.
Notice: The following is a collection of images that were colored and clipped by Disneyclips.com. Remember that they are meant for personal use only, and are not to be taken and displayed elsewhere online as part of a collection without providing a source link. The images are © copyrighted by Disney, all rights reserved. Help support Disneyclips.com by linking back.
Please read the Terms of Use
» Page 1 » Page 2 » Page 3
Back to School | Fall Season | Spring Season | Summer Season | Winter Season | Easter | Mother's Day | Father's Day | Christmas | Valentine's Day | Halloween | Weddings | Miscellaneous
More Easter
Check out Easter Fun for games, coloring pages and more!


text:
Let alone that people are using up the old technology. Little do they know if they were not so cheap that there are "good" Blackberry's to be had. Maybe this has something to do with the bad economy?
- Article | 52 min ago Skype for BlackBerry 10 updates and offers up a small selection of enhancements
- Article | 3 hours ago BlackBerry 'Dallas' and 'Venice' appear on India import records
- Article | 6 hours ago Zeus Browser brings Adobe Flash support back to your BlackBerry 10 smartphone
- Article | 10 hours ago BlackBerry 'Dallas' certified for Singapore and Malaysia
- Article | 12 hours ago Verizon begins OS 10.3.2.858 rollout for BlackBerry Q10, Z10 and Z30


text:
i need to place a backup database in my iphone. so i want any place outside my app folder where i can place the backup database so that if due to any problem my app crashes the user has a copy of his data.
any other idea except of placing data on a remote server is acceptable.


text:
H.R.495 - Free File Program Act of 2013113th Congress (2013-2014)
|Rep. Roskam, Peter J. [R-IL-6] (Introduced 02/05/2013)
|House - Ways and Means
|Latest Action:
|02/05/2013 Referred to the House Committee on Ways and Means.
This bill has the status Introduced
Here are the steps for Status of Legislation:
- Introduced
Subject — Policy Area:
- Taxation
- View subjects
Related Bills: H.R.495 — 113th Congress (2013-2014)All Bill Information (Except Text)
A related bill may be a companion measure, an identical bill, a procedurally-related measure, or one with substantive similarities. Bill relationships are identified by the House, the Senate, or CRS, and refer only to same-congress measures.


text:
"What they are delivering at the moment is not serious at all." That's Michael Fuchs, a senior lawmaker for German Chancellor Angela Merkel's Christian Democratic bloc and it seems to accurately reflect the sentiments of Greece's creditors with regard to the latest set of "proposals" submitted by Athens.
In the wake of Bankia bailouts to the tune of €19 billion, the second bailout in two weeks, yields of Spanish debt are soaring.
Spread to German Bonds Hits 5.05 Percentage Points
German utilities claim to be developing work-arounds that would provide Ukraine with gas in the event Russia cuts off supplies due to non-payment, or as part of an economic war against Ukraine. Now Germany's major energy utility companies are developing strategies to help Ukraine fill the shortfall if Moscow decides to cut gas supplies. Companies […]View the full post at: Germany Vows to Supply Gas to Ukraine: Piecrust Promise?
Did you hear the news? Germany, the world's second largest gold-holding nation, is recalling some of its gold. The Germans are bringing the physical metal – once on hold outside its borders – back in country.
This is a huge development in the world gold market. But more importantly may portend a life-changing trend that gold buyers like you and I can take to the bank.
Today let's connect a few more dots, and talk gold…
Germany, Russia, Ronald Reagan, Clausewitz, this story has it all. Let's start by covering a distant memory, the Cold War.
Cyprus is preparing for total financial collapse as the European Central Bank turns its back on the island after its parliament rejected a scheme to make Cypriot citizens pay a levy on savings deposits in return for a share in potential gas futures to fund a bailout. On Wednesday, the Greek-Cypriot government voted against asking its citizens to bank on the future of gas exports by paying a 3-15% levy on bank deposits in return for a stake in potential gas sales. The scheme would have partly funded a $13 billion EU bailout.
While corporate executives and the foundering economy remain prime targets in late-night talk-show monologues, the federal bailout is starting to take some lumps, too.
"President Obama is getting very tough now. He has imposed a $500,000 salary cap for executives getting Federal bailout money. And listen to this -- not only that, on weekends, they can only play miniature golf. No more 18 holes."
--Jay Leno
Bill Gross, Nouriel Roubini, Laurence Kotlikoff, Steve Keen, Michel Chossudovsky, the Wall Street Journal and many others say that our entire economy is a Ponzi scheme. Former Reagan budget director David Stockton just agreed:
Germany's largest party, Chancellor Angela Merkel's Christian Democrats, are overwhelmingly in favour of the deal negotiated to extend Greece's bailout package, according to Reuters.
By David White:Dr. Ben Bernanke has many times reiterated that the Great Depression occurred because too little action was taken too late. This seems to be the path the EU is following. Each time someone suggests having the ECB print money or puts forward the idea of euro bonds, Germany puts the kibosh on the idea. It is becoming obvious that Germany will likely balk at any of the more radical actions until any action is "too little too late." What is the solution?
Bullfax.com - Market News & Analysis 2008-2011 Contact Us | About Us | Terms & Conditions


text:
Cover Story, Chantal Vallee - Giving Windsor a New Sense of Pride. By D.J. Telfer.
Give an artist a blank canvass or a musician an instrument to play and turn talent and imagination into beautiful sights and sounds. Give a visionary director a dedicated cast and turn fiction into reality. Give Chantal Vallée a desperately bad basketball team and turn a doormat into a dynasty.
When Chantal Vallée decided to come to the University of Windsor and take over the women basketball program in 2005 there were many who thought she was making the wrong decision.
Seven consecutive OUA West Division titles and (count them) five straight CIS National Championships later, not only was it the right decision, it's historical.
Only one other team, the Laurentian Voyageurs, has ever won five consecutive CIS women titles and that was accomplished in the late 1970s.
Now the 2014-15 U of W women basketball team share those honours. It is a historical achievement and leaves no doubt the U of W women basketball program has been developed into the best in the country. And there is no doubt the credit for this goes to Chantal Vallée. Which leaves just a couple of questions: will the coach be back next year and can the women Lancers joust their way to one more history-making title?
"Yes," said Chantal in answer to the first question but she couldn't hide the growing smile tugging at the corners of her mouth, "as of now, yes I'll be back next year."
Hidden behind that smile was an open door. The future of the most successful coach in the history of U of W women basketball is not to closed to possibilities. If the right opportunity were to walk in, say, coach of the Canadian national team, not only would Chantal be compelled to accept (it would be another dream come true) she is deserving of the opportunity.
And to the question of winning again next year – well she would not rule it out but she's too smart to make any guarantees except one.
"As long as I stay at Windsor we will be successful." The confidence in her voice was as steady as the look in her eyes. "This is who we are now," she continued, "we are not going to change."
In this year's championship game the Windsor Lancers defeated the McGill Martlets 60-47. The national finals were held in Quebec City where Chantal lived for ten years. She was more than familiar with her surroundings – it felt like coming home for the Montreal native – and she is a graduate of McGill University which made winning this year's championship all the more especial. Of course the whole five-in-row thing was kind of cool too.
"Wouldn't it be awesome to win the fifth one in Quebec!" She said reflecting back. It was a thought that had been lingering in her mind since the start of the season so when it happened and against her alma mater, it was another dream come true and even the ever stoical Chantal was overwhelmed by her emotions.
"Right after the game I was hugging one of our coaches and I just started crying." she said. "I usually keep in control and I was surprised I was crying...but it was the excitement of the moment... it was all just a very emotional."
In the final game, the team was led by seniors Korissa Williams and Jocelyn LaRocque who became the first individual players to hoist the Bronze Baby trophy five straight times in nearly 40 years. Williams who was voted tournament MVP for the second time in three years (2013) led all scorers with 21 points. She also gathered in 14 rebounds while adding seven assists and six steals.
The Lancers were the number 1 rated team in the country and posted a 19-1 record this season. During their drive for five the team amounted an incredible 102-5 record.
This remarkable turnaround from a perennial disappointment to a national powerhouse is the stuff of legends. And no one dared to dream it except of course, Chantal Vallée but even she admitted there were moments when the challenge of winning a national championship appeared too daunting.
"In the beginning the dream seemed impossible." she said but she insisted that no matter how impossible the dream might have seemed she was entirely committed and even more, she believed it. She said the key was maintaining "a clear direction."
That direction included setting a new mind-set, a new perspective for players, coaches...the entire program.
"We needed to change the culture here," she explained. "we wanted to show our sincerity for transforming the program...and we needed everybody to see we were going to win at some point."
Coach Vallée came to Windsor as the youngest coach in the CIS. She came despite the criticism and despite the woeful past of the women basketball program. She came with a five-year plan and with full intentions of bringing a national championship to Windsor. She came unafraid of the challenges and she came here because she could imagine winning here.
History would have begged to differ.
In the two seasons prior to Chantal's arrival the women Lancers posted an 8-36 win-loss record. There were no banners hanging from the rafters. They had never competed for the CIS trophy, they had never once appeared in the national tournament.
To create a new picture, to play a new tune, to act differently, coach Vallée painted her own vision for the program. She emphasized core values which were (and still are) more about life skills than basketball skills.
With a Master's Degree in Sports Philosophy from McGill, Chantal said her own doctrine while inclined toward realism also embraces the abstract, she said, "it's not about sports, it is all based in character."
In other words, she believes the commitments and dedication to sport should be mirrored in life. The intangibles are the same and interchangeable, she believes success is not measured by winning but calculated in respect and integrity on and off the court.
Still, considering her women Lancers have ridden rough-shod over the entire league for the past five years others might not regard the experience as a cordial intercourse, but it has been delightful to watch.
After noticeable improvements the first two years (but no playoffs) the third year yielded a peek into the excitement yet to come for the women. The team went a respectable 14-8 and made it to the playoffs but did not advance further. However the momentum was building and the team coach Vallée was still building were embracing her vision and beginning to believe in themselves.
In 2008-2009 the Lancers churned like a whirlwind and they ripped the league apart posting a sparkling 21-1 record. They had finally made it to the big-dance but these young upstarts were not quite ready to live at the top and finished fourth in the national tournament.
The next year was nearly the same, they only lost two regular season games and made it to the finals again but lost in the championship game. But in that defeat the women learned how to win.
In 2011, a season of beautifully choreographed performances the once dejected, defeated and lowly Lancers, a team which had been lying like a doormat out on the stoop for these many long years were ready for top billing; and they were notabout to tap lightly on the door and politely ask to come in. Instead, the 2011 Women Lancers kicked the door right off its hinges and moved into the CIS penthouse. They have been there ever since.
"You have to have vision." Chantal had said at the beginning of our interview. "and then you have to bring that vision to term."
So after six years of molding and shaping. Six years of adding different colours, different music, different characters, Chantal had brought her visions to term and her dreams had come true. She had her masterpiece. Now what?
She thought about leaving.
"After all the excitement was over I felt a huge crash," she admitted. "I felt lost. I had been on a six year rush and now it was over. I wasn't sure what I wanted to do."
Well it did not take long for her to shake off the melancholy and let her dreams and imagination rise again. Once she started to focus on next year the visions grew clearer and like an artist she embraced the challenge of painting another masterpiece. No it did not take too long for her to dream about winning it all again and to feel the rush begin to build once more. The rest as they say, is history.
The accomplishments of the women Lancer basketball teams and the coaching of Chantal Vallée have garnered national attention. It is no secret Chantal is regarded by many as the best coach in the country and it would be hard to dispute it. There are many who court her favour including American suitors. Indeed, the door is open to possibilities and so it should be. Although winning five consecutive championships should be more than enough to satisfy any coaching career, Chantal is not just any coach and she is far from the end of her career. She still has much more to achieve.
Chantal has vision and creativeness that transcends sports "it's about the human spirit...the human being and character" she said. "Sport is just a part of life."
She has the passion every coach would claim but very few possess and she has a natural ability to turn nothing into something, something into extraordinary and the extraordinary into the sublime.
Her personality, her confidence, her skills as a coach and teacher; as a mentor and leader; and her successes at Windsor are now legendary but of course there is so much more to write about Chantal Vallée. There are her Olympic experiences, her involvement with the national team, her 2014 Coach of the Year honours and her future aspirations to name just a few. But her legacy is not yet to be written – there are many chapters still to be scripted in the life and career of Chantal Vallée.
And if or when the day comes and she chooses to leave, her decision will be the right one and will not in any way detract from her love for Windsor or for her lady Lancers. It will not diminish her enduring accomplishments or her historical achievements.
"I am so proud to have achieved this in Windsor." Chantal said affectionately, "and I am proud to have brought greater recognition for the University and for women basketball."
Last, but hardly least. It's true, winning is addictive but with every win, with every title the desire and the pressure to win again grows exponentially. To all the U of W women basketball players who have been a part of this amazing run; grinding out the regular seasons year after year; staying focused and centred through-out the post-seasons, keeping composed under the mounting pressures, well done!
Not only have you given great honour to yourselves and the University of Windsor, you have given the entire city a new sense of pride.


text:
this car was built by classic roadsters, it is in near perfect condition, 302 ford engine, 4 speed transmission, 9"ford rear has removable hardtop, A/C... More
Custom wide body Austin Healey re-creation Full sqaure tube chassis Chrome roll bar 350 CID V8 Curry built 9″ rear end Rack and pinion steering Disc brakes Custom 18″ front and... More
Price reduced! All vehicle trades considered, including classics and performance. Here is a great car for the summer. This 1963 Austin Healey 3000 replica captures... More
1965 AUSTIN HEALY SEBRING REPLICA CONVERTIBLE - FOR MANY MORE DETAILS PLEASE CALL 732-735-0125 MANUFACTURED BY CLASSIC ROADSTERS IN FARGO NORTH DAKOTA THE BEST OF ALL THE... More


text:
- 800.878.7323
Oracle SQL Plus the Definitive Guide 1ST Editionby Jonathan Gennick
Synopses & Reviews
Publisher Comments:
This book is the definitive guide to SQLPlus. If you want to take best advantage of the power and flexibility of this popular Oracle tool, you need this book.SQLPlus is an interactive query tool that is ubiquitous in the Oracle world. It is present in every Oracle installation and is available to almost every Oracle developer and database administrator. SQLPlus has been shipped with Oracle since at least version 6. It continues to be supported and enhanced with each new version of Oracle, including Oracle8 and Oracle8i. It is still the only widely available tool for writing SQL scripts. Despite this wide availability and usage, few developers and DBAs know how powerful SQLPlus really is.This book introduces SQLPlus, includes a quick reference to all of its syntax options, and, most important, provides chapters that describe, in step-by-step fashion, how to perform all of the tasks that Oracle developers and DBAs want to perform with SQLPlus — and maybe some they didn't realize they COULD perform with SQLPlus. You will learn how to write and execute script files, generate ad hoc reports, extract data from the database, query the data dictionary tables, customize your SQLPlus environment, and use the SQLPlus administrative features (new in Oracle8i).This book is an indispensable resource for readers who are new to SQLPlus, a task-oriented learning tool for those who are already using it, and a quick reference for every user.A table of contents follows:
A guide to SQL*Plus, Oracle's interactive query tool. The text introduces SQL*Plus, provides a syntax quick reference, and describes how to develop and test SQL queries, generate ad hoc reports, execute script files, extract data from the database, query the data dictionary tables and more.
This book is the denitive guide to SQL*Plus, Oracle's interactive query
This definitive guide to SQL*Plus, Oracle's interactive querytool, shows Oracle developers and database administrators how totake full advantage of the power and flexibility of this populartool. Despite the wide availability and usage of SQL*Plus, fewdevelopers and DBAs know how powerful it really is. This book introduces SQL*Plus, includes a quick reference to itssyntax options, and provides chapters that describe, step-by-step, how to perform all the tasks Oracle developers and DBAs want toperform with SQL*Plus — and maybe some they didn't realize theyCOULD perform. You will learn how to write and execute script files, generate ad hoc reports, extract data from the database, query the data dictionary tables, customize your SQL*Plus environment, and use the SQL*Plus administrative features (new in Oracle8i)."Oracle SQL*Plus: The Definitive Guide is an indispensableresource for readers who are new to SQL*Plus, a task-orientedlearning tool for those who are already using it, and a quickreference for every user.
About the Author
Jonathan Gennick is an O'Reilly & Associates editor specializing in database and programming titles. Prior to joining O'Reilly, Jonathan amassed some 17 years of programming and database management experience. During the latter part of his career he specialized in relational database programming, and more recently in database management. Jonathan got his start with relational database systems in 1990, first working with Ingres, and later with Digital's Rdb software. During that period he developed a fondness for SQL, and for the challenge of applying SQL creatively in ways that leveraged it's set-oriented capabilities. In 1994 Jonathan made the leap to Oracle, and is now often found writing about it. Recent projects include Oracle SQLLoader (O'Reilly & Associates, 2001); Oracle SQLPlus Pocket Reference (O'Reilly & Associates, 2000); Oracle SQL*Plus: The Definitive Guide (O'Reilly & Associates, 1999); More recently, Jonathan has made forays into other database products, coauthoring Transact-SQL Cookbook (O'Reilly & Associates, 2002) and editing Practical PostgreSQL (O'Reilly & Associates, 2002). Jonathan is certified as an Oracle DBA and is a member of MENSA and the Oracle Technology Network. He holds a Bachelor of Arts degree in Information and Computer Science, with a Business Administration minor, from Andrews University in Berrien Springs, Michigan. Jonathan currently resides in Munising, Michigan with his wife Donna and their two children: Jenny and Jeff. Jonathan may be contacted by email at email@example.com, and you can learn more about him personally by visiting his website at http://gennick.com.
Table of Contents
Preface; Why I Wrote This Book; Objectives of This Book; Structure of This Book; Audience; Obtaining the Scripts and Sample Data; Conventions Used in This Book; Which Platform and Version?; How to Contact Us; Acknowledgments; Chapter 1: Introduction to SQL*Plus; 1.1 What Is SQL*Plus?; 1.2 History of SQL*Plus; 1.3 Why Master SQL*Plus?; 1.4 Creating and Loading the Sample Tables; Chapter 2: Interacting with SQL*Plus; 2.1 Starting SQL*Plus; 2.2 Some Basic SQL*Plus Commands; 2.3 Running SQL Queries; 2.4 Working with PL/SQL; 2.5 The SQL Buffer; 2.6 Line Editing; 2.7 Executing the Statement in the Buffer; 2.8 Saving and Retrieving the Buffer; 2.9 The EDIT Command; 2.10 Executing a Script; 2.11 The Working Directory; Chapter 3: Generating Reports with SQL*Plus; 3.1 Following a Methodology; 3.2 Saving Your Work; 3.3 Designing a Simple Report; 3.4 Advanced Report Formatting; 3.5 Totals and Subtotals; 3.6 Other Reporting Topics; Chapter 4: Writing SQL*Plus Scripts; 4.1 Why Write Scripts?; 4.2 Using Substitution Variables; 4.3 Prompting for Values; 4.4 Cleaning Up the Display; 4.5 Packaging Your Script; 4.6 The DEFINE and UNDEFINE Commands; 4.7 Controlling Variable Substitution; 4.8 Commenting Your Scripts; Chapter 5: Extracting and Loading Data; 5.1 Types of Output Files; 5.2 Limitations of SQL*Plus; 5.3 Extracting the Data; 5.4 Reloading the Data; Chapter 6: Exploring Your Database; 6.1 The DESCRIBE Command; 6.2 Oracle's Data Dictionary Views; 6.3 Tables; 6.4 Table Constraints; 6.5 Indexes; 6.6 Triggers; 6.7 Synonyms; 6.8 Table Security; 6.9 Finding More Information; Chapter 7: Advanced Scripting; 7.1 Bind Variables; 7.2 Branching in SQL*Plus; 7.3 Looping in SQL*Plus; 7.4 Validating and Parsing User Input; 7.5 Error Handling; Chapter 8: Tuning and Timing; 8.1 Using SQL*Plus Timers; 8.2 Using EXPLAIN PLAN; 8.3 Using AUTOTRACE; 8.4 Improving on EXPLAIN PLAN Results; 8.5 Where to Find More Tuning Information; Chapter 9: The Product User Profile; 9.1 What Is the Product User Profile?; 9.2 Using the Product User Profile; Chapter 10: Administration with SQL*Plus; 10.1 Connecting for Administrative Purposes; 10.2 Starting and Stopping a Database; 10.3 Looking at Your Database; 10.4 Database Backup and Recovery; Chapter 11: Customizing Your SQL*Plus Environment; 11.1 SQL*Plus Settings You Can Control; 11.2 The Site and User Profiles; 11.3 Environment Variable Settings; 11.4 The SET Command; 11.5 The SHOW Command; SQL*Plus Command Reference; SQLPLUS; Comment Delimiters (/*...*/); Double Hyphen (- -); At Sign (@); Double At Sign (@@); Forward Slash (/); ACCEPT; APPEND; ARCHIVE LOG; ATTRIBUTE; BREAK; BTITLE; CHANGE; CLEAR; COPY; COLUMN; COMPUTE; CONNECT; DEFINE; DEL; DESCRIBE; DISCONNECT; EDIT; EXECUTE; EXIT; GET; HELP; HOST; INPUT; LIST; PASSWORD; PAUSE; PRINT; PROMPT; QUIT; RECOVER; REMARK; REPFOOTER; REPHEADER; RUN; SAVE; SET; SHOW; SHUTDOWN; SPOOL; START; STARTUP; STORE; TIMING; TTITLE; UNDEFINE; VARIABLE; WHENEVER; SQL*Plus Format Elements; Formatting Numbers; Formatting Character Strings; Formatting Dates; Colophon;
What Our Readers Are Saying
Add a comment for a chance to win!
Average customer rating based on 1 comment:
Product Details
Other books you might like
Related Subjects
Computers and Internet » Computer Languages » SQL


text:
Tim Papadopoulos, BSc, MBBS, FRACS
Ratings based on 35 reviews
Write a review
Are you a patient of Tim Papadopoulos, BSc, MBBS, FRACS?
Reviews (35)
5 out of 5 stars Body Lift
Perfection - Lane Cove, AU
Show More Dr Tim is passionate caring and empathetic about the physical & emotional outcome of what he can do for his patients. He was completely transparent when discussing my procedure and realistic about what could be achieved. As a result of the pre-op consultations, I felt informed, comfortable and at ease about my decision to go ahead with the surgery. The procedure I ended up deciding to have was more comprehensive, however the results are very much worth it. As quoted in Dr. Tim's profile, I believe that he never leaves the operating theatre until, " the results are the absolute best they can be". A huge thank you Dr. Tim. Your artistic skills, techniques & knowledge have far outweighed my expectations.
5 out of 5 stars Tummy Tuck
Tummy Tuck and Repair of Stomach Muscle Separation of 10 Cm - Penrith, AU
5 out of 5 stars Breast Reduction
Tummy Tuck, Breast Lift and Lipo - Penrith, AU
Show More Dr. Tim and his staff were all very supportive and I've never felt more comfortable. Dr. Tim has an eye for detail and I would strongly recommend anyone considering surgery first go and see Dr. Tim before making a secure decision. Not one cent was wasted!!! It is the best investment I've ever made.
5 out of 5 stars Breast Reduction
Dr Tim - Highly Skilled - Sydney, AU
Show More Heard about him on the radio, and a relative also had hand surgery by Dr Tim (he's also a specialist in hand surgery for injuries etc), Dr Tim has an incredible bedside manner and knows all there is to know about cosmetic surgery. The ladies that work for Dr Tim are so lovely and also very knowledgable.
5 out of 5 stars Mole Removal
Dr Tim Potentially Saved my Life - Kingswood, AU
Show More Dr Tim was simply sensational. He explained the processes to me and was informative and extremely comforting and reassuring during this difficult and confronting period. I would recommend Dr Tim to anyone having any type of procedure done. Not only is he highly skilled and professional, he's an absolute gentleman.
5 out of 5 stars Breast Implants
34 with 2kids - Penrith, AU
Show More would highly recommended to see drtim extremly happy with what he did for me & he has put a big smile on my face!!!!!
5 out of 5 stars Mommy Makeover
31 Years Old, Tummy Tuck, Breast Lift with Implants - Lane Cove, AU
Show More Dr Tim is an amazing,professional and respectful Surgeon. He has exceed my expectations and I couldn't be happier. I felt cared for and understood. He had patience and listened to my concerns. An absolute gentleman, thank you Dr Tim and Cheryl use have been a great support.
5 out of 5 stars Eyelid Surgery
Eyelid Surgery and Sub Cutaneous Brow Lift - Penrith, AU
Show More Cannot thank Dr Tim enough. The whole process from start to finish and in between was a pleasure. Dr Tim is the kindest most informative Dr and the results of the surgery are nothing short of amazing. Dr Tim and his staff are the reason I will never go elsewhere. Highly recommended cant wait to go back!
5 out of 5 stars Body Lift
46yr Female, Armlift and Full Body Lift - Penrith, AU
Show More Dr Tim was fantastic, very professional and caring, and I couldn't fault him or his work, his staff in any way
There is no recent activity.


text:
You are a fan of year muvizu ? Come to discover the best forums year muvizu on the internet and share your experience with a community fan of year muvizu . You can also build a forum year muvizu and create your online discussion.
Year is Year
Free forum : The year is 2020, over two decades of hard-won peace throughout the wizarding realm.
This is a South Park Role Playing Forum set in the year 2017, over six years after the original South Park Storyline.
An entertainment forum. A community for movie reviews and discussion, entertainment news, celebrity gossip and TV shows.
entertainment, movies, gossip, news, celebrities, movie, releases, cancelled, shows, production, soundtracks, music, album, #year, oscars, famous, actors
We are a Md based club of folks that like going to shows, cruise ins and events all over the Mid Atlantic area, with an interest in Ford Mustangs and All Ford products of any year, This i
Come join the one of the best Call of Duty communities/clans there is! We are active and still going strong after a whole year!
The new sister site of Lassongra and Dog Days. A mature all feral roleplay for 18+ year olds. No location, no weather and no limitations, you are able to have full range to roleplay and make it your own.


text:
Create an online shopping cart store in minutes. Make sales on every page of your website with the Frame Webstore Toolbar.
A plugin for WordPress charity fund raiser, an html5 canvas thermometer. The html5-canvas and JS is a credit to Ray Hammond who implements the canvas
Now you can sell your blog articles direct to customers using eXopin, a free plug-in which collects payment and seamlessly transfers content.
WP DealPixel Ads - allows you to list DealPixel.com deals in your sidebar and make money with the DealPixel affiliate network.
Simple Skrill / Moneybookers plugin
Make money on your website. Adds a "Shop" button to your page. Make money on purchases.
A simple yet advanced, intuitive, easy-to-use and complete currency converter plugin with a beautiful unique converter tooltip.
Version: 1.1 Stable
Enrich your content and earn money per click with the best personalized mobile application recommendations technology
Adds planwise iframe via a shortcode or via a page template
Currency calculator plugin, converts money amounts between different currencies.
The Fashion Traffic plugin makes it easy to search for and post photos with affiliate links of your favorite fashion items without leaving WordPress.
Let ALL of your blog content earn you money.
Piccshare is a sharing button that will be rendered on top of each photo and will expose social share buttons to enable sharing the photo link to face
Shows the official currency exchange rate from US Dollars to Costa Rica Colones.
Magic Floating Sidebar Maker, makes your bog sidebar floatable.
AdPop is an advertising program which let you make money without taking any ad space.
CONTAXE-Werbemittel in Ihren WordPress-Blog einbinden.
Simple to use invoicing system that can intergrate with Paypal. Very simple, very flexble.
Tested on: 3.2.1 Before It's News®: Money Widget updates every time our site does. A new story is added virtually every minute!
Database result serialization.
Wishpot Publisher Pro© allows you to easily add product based ads to your wordpress powered website as widget in a sidebar or as pages.
An Affiliate Marketers dream! Promote products automatically from iDavi with your affilaite ID. Make money 24 hours a day on complete auto-pilot.
Send and receive money through Twitter.
Make Money Calculator is a simple to use calculator that calculates how much money you need to make (in different time intervals) in order to achieve
Monetize your blog by offering your visitors/readers to buy advertising space on your blog.
Add sleek looking Amazon mp3 widget with best selling music to your blog posts by just adding the word [mp3].
The ShareMacro widget empowers your visitors to email, bookmark and share your site with services, such as Facebook, Twitter, StumbleUpon, Digg..
Cent2Cent for WordPress helps you to make money with your digital content. It allows you to easily charge for your text, images and videos.
Plugin to display/insert Custom code (PHP, HTML, Javascript), announcements, Video's, adsense, affiliate/referrals on your blog's front page


text: This hotel room was quite nice! I stayed there on business for a weekend. The room is surprisingly large which was amazing!!!! The room was clean and well-lit. One thing I did not like- the service from the staff was HORRIBLE. I left my things in the room past checkout time; being that I had to go to a business... More


text:
Approximate end time: 9:50pm
New York comes to London for a musical-theatre extravaganza from the Royal Philharmonic Orchestra: the classic hits from the Broadway shows. Experience the razzmatazz of theatre's classics in this star-studded evening set in the glamorous surround of the Royal Albert Hall.
Your favourite musicals recreated by London's favourite orchestra, including hit songs from The Phantom of the Opera, Miss Saigon, Cats, Les Misérables, Chicago, Mamma Mia, Billy Elliot, Wicked and many more!
New for 2015: hit numbers from the classic West Side Story, including Somewhere, Tonight and Maria.
Presented by vocalists Mary Carewe, Ria Jones, Michael Dore and Graham Bickley, with Music Director Nick Davies.
Part of
Royal Philharmonic Orchestra: 2014-15 Season


text:
India's ruling Congress Party promised on Friday that K. Siddaramaiah would bring "transparent, corruption-free" government to the state. Read More »
May 10, 2013
India's ruling Congress Party promised on Friday that K. Siddaramaiah would bring "transparent, corruption-free" government to the state. Read More »
Voters in Bangalore tell India Real Time who they voted for in the state election and why. Read More »
Capturing Karnataka means snaring its capital Bangalore, India's IT hub. Politicians are looking on the vote as a qualifying match before national elections.
By Joanna Sugden
For devadasis, which means female servants of god, puberty is the moment they are supposed to begin a life supporting themselves and their families by having sex. Read More »
A new ruling should in theory lay to rest a century-old dispute over the waters of the Cauvery River in south India, but that is an optimistic view. Read More »
Groups representing Dalits are opposed to plans to even temporarily shift a statue of their hero, B.R. Ambedkar, to allow for the construction of an underground metro railway in Bangalore. Read More »
India Inc. is losing millions of rupees in potential revenue every year because of power cuts, a new survey reveals.
By Debiprasad Nayak and Biman Mukherji
India declared itself free of bird flu late last year. But the recent outbreak of the virus at a government facility in the southern state of Karnataka has raised worries this could cause significant damage to the poultry industry. Read More »
At a time when the bottom has fallen out of the global iron ore market, prices of the commodity have slipped by a much smaller extent in India.
The reason? Severe restrictions in the local supply of iron ore, the key raw material in making iron and steel. Read More »
Girisha Hosanagara Nagarajegowda opened India's medal tally at the 2012 Paralympic Games in London, clinching silver in the men's high jump finals. Read More »
India Real Time offers analysis and insights into the broad range of developments in business, markets, the economy, politics, culture, sports, and entertainment that take place every single day in the world's largest democracy. Regular posts from Wall Street Journal and Dow Jones Newswires reporters around the country provide a unique take on the main stories in the news, shed light on what else mattered and why, and give global readers a snapshot of what Indians have been talking about all week. You can contact the editors at indiarealtime(at)wsj(dot)com.
Check out the main contributors to the blog and their bios here.
Content engaging our readers now, with additional prominence accorded if the story is rapidly gaining attention. Our WSJ algorithm comprises 30% page views, 20% Facebook, 20% Twitter, 20% email shares and 10% comments.


text:
- The week in security: Australian governments, telcos least trusted to protect personal data
- Ad fraud Trojan updates Flash Player so that other malware can't get in
- Cisco leaves key to all its Unified CDM systems under doormat
- Integrate encryption with device management to overcome key-management issues: LANDESK
- Plex hacker demands Bitcoin ransom for return of data
Building a Better Mousetrap in Anti-Malware
This story is becoming frustratingly old. Cyber threats are continuously advancing in their adaptability speed, sophistication, and degree of stealthiness. At the same time, the exposed footprint is expanding. More business operations are moving online and end-user devices—corporate-issued and user-owned—are expanding in number and variety. A reasonable question asked by executives responsible for making decisions on their organisations' security budgets is whether their money and resources are being spent wisely. Are their businesses buying and using the best mix of security technologies to meet their needs and obligations? Read on.
Email archiving is a little bit like driving your reliable car. You expect it to operate smoothly, day after day, and do not necessarily plan for any crisis events such as a sudden engine failure. That is about the same perception many enterprises have about email. They don't worry about backup, about archiving, about eDiscovery, until, as one IT administrator said, something goes wrong. As the CIO whose diverse responsibilities includes budget control, cost avoidance, risk mitigation, data management and supporting the operational excellence of the enterprise as a whole, email archiving may not always be top of mind. Until something goes wrong. Read this whitepaper to learn how next-gen email archive delivers true business value. Download whitepaper »
According to IDC, sixty percent (60%) of business critical information is now stored, sometimes exclusively, in email. It۪s more important than ever to establish an email archiving strategy that ensures business continuity and information availability. Modern email archiving solutions are changing to accommodate the new realities of the digital workplace and can intelligently store, manage and discover email content to meet business and compliance requirements. Read this paper to learn how to solve your top email challenges. Download whitepaper »
This infographic compares legacy backup solutions with modern data protection solutions, showing legacy backups shortcomings in pivotal areas of data protection, such as application awareness, support for cloud tiers and more. Check out this infographic to learn why modern data solutions deliver more benefits than legacy backups. Download whitepaper »
- Digital Pacific ramps up web hosting mite with Crucial buy
- 2015 ARN ICT INDUSTRY AWARDS: Channel Choice opens, get voting
- 2015 ARN ICT Industry Awards: Nominations hit 460 smashing the old record
- UXC Oxygen appoints Craig Stevenson as business manager
- Ransomware in Australia has seen a steady growth in the past six months: Bitdefender
- Digital Pacific acquires Crucial
- Uber throws in the towel in battle with French taxi drivers
- As Windows 7 breaks the 1B-device mark, Microsoft's challenge will be to force it back to zero
- Android phone vendors should improve update policies, consumer organization says
- Five smartphones to look forward to


text:
SCOTTSDALE, Ariz. (AP) - The San Francisco Giants say some of the baseball club's minor league players were treated for minor injuries after a traffic collision in Scottsdale, Ariz.
SYRACUSE, N.Y. (AP) - Trevor Cooney scored 21 points, Tyler Ennis added 17 points and eight assists, and No. 4 Syracuse beat Indiana 69-52 in the Big Ten/ACC Challenge on Tuesday night.
TORONTO (AP) - Mike Brown had a goal against his former team and Joe Thornton scored on a 5-on-3 power play, leading the San Jose Sharks to a 4-2 victory over the Toronto Maple Leafs on Tuesday night for their sixth straight win.
NEW YORK (AP) - Free agent outfielder Jacoby Ellsbury, fresh off winning the World Series with Boston, reached agreement with the rival New York Yankees on a seven-year contract worth about $153 million, a person familiar with the negotiations said Tuesday night.
GREEN BAY, Wis. (AP) - The Green Bay Packers aren't ruling out Aaron Rodgers for Sunday's game against the Atlanta Falcons even though the star quarterback hasn't yet been medically cleared to play since breaking his left collarbone a month ago.
SEATTLE (AP) - Pete Carroll gathered his team in the locker room and finally offered some congratulations.
SEATTLE (AP) - Seahawks fans rocked the stadium so hard during Monday night's matchup with New Orleans that seismic instruments registered small tremors at various points in the game.
PITTSBURGH (AP) - Mike Tomlin was "mesmerized."
NEW ORLEANS (AP) - A long night on the field in Seattle got even worse off of it, and now the Saints are operating on a compressed time-frame as they brace for surging Carolina with first place in the NFC South at stake.
THOUSAND OAKS (AP) - Of all the memories from Tiger Woods' roots in Southern California, it's easy to overlook the time he made an appearance in the Tournament of Roses parade.
MOON TOWNSHIP, Pa. (AP) - Robert Morris University is cutting seven Division I athletic programs to bolster funding for the remaining 16 sports.
BEAVER CREEK, Colo. (AP) - Early in his career, Andrew Weibrecht constantly flirted with the line between foolhardy and fast on a downhill course.
LAS VEGAS (AP) - O.J. Simpson's lawyers said Tuesday they will take his case back to the Nevada Supreme Court, hoping justices will overturn a lower court judge's decision last week denying the former football stat a new trial in his armed robbery case.
The chairman of the U.S. Olympic Committee says "it is our intention to bid" for the 2024 Olympics if a number of criteria are met - the strongest indication yet that the United States will put forth a bid city by the end of next year.
LAKE LOUISE, Alberta (AP) - Lindsey Vonn was cleared to get back on a World Cup course for downhill training, the latest step as she works to return from a right knee injury and get ready for the Sochi Olympics.
Page 275 of 604
LOS ANGELES (AP) - Los Angeles Clippers forward Matt Barnes was fined $50,000 by the NBA on Friday for directing inappropriate language at a courtside fan who turned out to be Houston star James Harden's mother.
NEW YORK (AP) - Ryan McDonagh scored 9:37 into overtime and the New York Rangers kept their Stanley Cup hopes alive with a 2-1 victory over the Washington Capitals on Friday night in Game 5 of the Eastern Conference semifinal.
PONTE VEDRA BEACH, Fla. (AP) - The biggest star at The Players Championship was not Rory McIlroy or Jordan Spieth playing together in the morning. And it wasn't Phil Mickelson and Tiger Woods in back-to-back groups in the afternoon.
CHICAGO (AP) - There was a time when Jimmy Butler was kicked out of his home as a teenager with no place to go. He has come a long way since then.
SOUTH LAKE TAHOE, (AP) - Mark Cavendish and Kristin Armstrong will highlight the Tour of California.
WASHINGTON (AP) - John Wall has five non-displaced fractures in his left wrist and hand, and the All-Star guard's playing status has not been determined.
NEW YORK (AP) - Floyd Mayweather said in an upcoming interview with Showtime that at this moment he's not interested in a rematch with Manny Pacquiao "because he's a sore loser and he's a coward."
ST. PAUL, Minn. (AP) - Corey Crawford made 34 saves, Patrick Kane had a goal and an assist, and the Chicago Blackhawks advanced to the Western Conference finals with a 4-3 victory over the Minnesota Wild on Thursday night for a four-game sweep.
SALEM, Mass. (AP) - New England Patriots quarterback Tom Brady said Thursday night the scandal surrounding his use of deflated footballs during the NFL playoffs last season hasn't detracted in any way from his Super Bowl title.
SAN FRANCISCO (AP) - On a night when he allowed more hits than any San Francisco pitcher in 47 years, two stood out to Tim Hudson.
MINNEAPOLIS (AP) - On a day when Drew Pomeranz had difficulty finding the plate, he sure could have used some help from his defense to grind through the series finale against the Minnesota Twins.
Page 35 of 35
Contents of this site are © 2015 Manteca Bulletin - All Rights Reserved
Privacy policy and Terms of service | Contact Us


text:
Dental Associates, Wisconsin's largest family-owned dental group practice, has been recognized by The Wellness Council of America and Wellness Council of Wisconsin, a local affiliate, with a Silver Well Workplace Award for implementing a comprehensive health and wellness program for its 800-plus employees and their family members.
In 2012, Dental Associates became of member of Well City Milwaukee with the goal of transforming its wellness program and earning a Well Workplace Award. To accomplish that, the company decided to provide convenient workplace and at-home access to health care professionals, individualized health education and ongoing wellness coaching, feedback and measurement.
"We're a healthcare company so it makes sense for us to provide a workplace structure that encourages health and well-being," says Anthony Vastardis CEO of Dental Associates. "Plus, we're continually working to be an employer of choice and feel that a comprehensive wellness program is an attractive offering."
As part of its employee health initiative, Dental Associates partnered with a local healthcare system to bring the expertise of nurse case managers to all 10 of its locations. The nurses work on-site and also conduct home visits with staff and their family members, providing health care education, disease management guidance and wellness coaching.
By achieving the Well Workplace Award Dental Associates has made a long-lasting commitment to the health and wellbeing of its employees.
Founded in 1973, Dental Associates is Wisconsin's largest family-owned dental group practice with 11 offices throughout the state and nearly 850 staff members, including 100 doctors and 494 operatories. Dental Associates provides complete family dental services, both general and specialty dentistry, under one roof – from pediatric dentistry to specialized dental services for older adults – with a focus on excellent care that is affordable, accessible and personalized. Dental Associates is accredited by the Accreditation Association for Ambulatory Health Care (AAAHC). Through the company's CarePlus Dental Plans, Dental Associates provides affordable and high-quality dental health care to all Wisconsin residents. For more information regarding Dental Associates, visit www.dentalassociates.com.
This site uses Facebook comments to make it easier for you to contribute. If you see a comment you would like to flag for spam or abuse, click the "x" in the upper right of it. By posting, you agree to our Terms of Use.
E-mail Newsletter
Your link to the biggest stories in the suburbs delivered Thursday mornings.
Enter your e-mail address above and click "Sign Up Now!" to begin receiving your e-mail newsletter Get the Newsletter!
More from Your Stories
- Waukesha Sunrise Rotary Looks for Golfers to Play The Legend of Brandybrook
- Free Art and Craft Fair on July 11
- Local Residents Participate in THOROUGHLY MODERN MILLIE at Sunset Playhouse
- Local Residents Participate in THOROUGHLY MODERN MILLIE at Sunset Playhouse
- Local Resident Particiaptes in THOROUGHLY MODERN MILLIE at Sunset Playhouse
- Local Residents Participate in THOROUGHLY MODERN MILLIE at Sunset Playhouse
- Independence Day Activities in Waukesha
- Greater Brookfield Chamber of Commerce announces monthly Tech Trends series of workshops on IT, internet safety, and commonly-used software
- Dr. Nicole Knapp, D.C. launches blog to educate general public about health conditions that can affect the quality of their life
- National "I Forgot Day"


text:
What is disclosed is a glycoprotein having proteolytic, fibrinolytic, and thrombolytic properties and which can be used to prepare anti-sera useful as diagnostic agents, said glycoprotein being found in human blood serum and extracts of human placentas and being capable of isolation therefrom and which has the following properties:
a protein proportion essentially consisting of 89±4% of α-amino-acids,
a carbohydrate proportion of 11.1±2.2%, among it 5.3±1.1% of hexoses, 2.8±0.5 N of N-acetylated hexosamine, 2.9±0.6% of N-acetylated neuraminic acid;
a sedimentation coefficient S20x of 3.2±0.3 S;
a molecular weight of 32 000±6 000;
an iso-electric point at pH 4.3±0.3;
an extinction coefficient E1 cm1% (280 nm) of 13.8±1.0;
an electrophoretic mobility in the range between that of albumin and α1 -globulins;
a specific immunologic reaction with an antibody specifically directed against the protein, and
a proteolytic activity, as well as a process for isolating it.
1. An isolated glycoprotein having the following amino acid analysis:
______________________________________ Frequency (Mole %) Variation Coefficient (%)______________________________________Lysine 0.67 39.25Histidine 3.36 9.97Arginine 5.09 6.58Aspartic acid 5.87 3.07Threonine 3.83 15.74Serine 7.84 8.22Glutamic acid 11.76 1.35Proline 6.46 1.71Glycine 10.24 4.49Alanine 11.25 3.76Cystine/2 5.10 6.34Valine 6.87 5.18Methionine 0.42 14.48Isoleucine 2.47 5.07Leucine 11.42 4.23Tyrosine 2.95 5.45Phenylalanine 2.42 13.52Tryptophan 1.98 11.37______________________________________
and the following properties:
(a) a protein content of 89±4%;
(b) a carbohydrate content of 11.1 ±2.2%; including 5.3±1.1% of hexoses, 2.8±0.5% of N-acetylated hexosamine, and 2.9±0.6% of N-acetylated neuraminic acid;
(c) a sedimentation coefficient, S20 w, of 3.2±0.3 S;
(d) a molecular weight of 32,000±6000;
(e) an isoelectric point of pH 4.3±0.3;
(f) an extinction coefficient, E1 cm1% (280 nm), of 13.8±1.0;
(g) an electrophoretic mobility in the range between albumin and the α1 -globulins;
(h) a specific immunologic reaction with an antibody directed specifically against the glycoprotein;
(i) proteolytic activity.
2. A method for purifying a glycoprotein as in claim 1 which comprises fractionating a protein solution in which the presence of the glycoprotein can be demonstrated immunologically and isolating that fraction which contains the glycoprotein, said fractionating comprising at least one of the following steps:
(a) adding neutral salts to said protein solution until the glycoprotein is precipitated;
(b) fractionating said protein solution on a molecular sieve and isolating the fraction having a molecular weight between 25,000 and 40,000;
(c) adsorbing the glycoprotein from said protein solution onto a weakly basic ion exchanger and then eluting it therefrom;
(d) adding a water-soluble derivative of an acridine or quinoline base to said protein solution in a pH range from 5 to 10 until a final concentration of about 0.8% is reached, whereby the glycoprotein remains in said protein solution;
(e) treating said protein solution with hydroxyl-apatite, whereby said glycoprotein remains in said protein solution;
(f) subjecting said protein solution to preparative zone electrophoresis and isolating the zone between albumin and the α1 -globulins; or
(g) treating said protein solution with an immuno-adsorbant.
3. A method as in claim 2 wherein said protein solution in which the presence of said glycoprotein can be demonstrated immunologically is an extract of human placentas.
4. The method of making an antiserum to the glycoprotein as in claim 1, which method comprises immunizing a vertebrate animal with said glycoprotein and then isolating that fraction of the animal's serum which contains antibodies against said glycoprotein.
5. An antiserum made by the method of claim 4.
The present invention relates to a new glycoprotein which can be found in the blood serum and in extracts of human placentas and which can be isolated therefrom, as well as to a process for isolating it.
It is known that the protein solution obtained by aqueous extraction of human placentas contains a great number or components, which are partly serum proteins and on the other hand tissue proteins.
The task set for the present invention was to isolate a hitherto unknown glycoprotein from an extract of human placentas, to prepare with it antiserums which are specifically directed against the new glycoprotein and which permit proving the presence of the new glycoprotein in the serum qualitatively or determining it quantitatively.
Thus, the object of the invention is a new glycoprotein which can be isolated from the blood serum and the extract of human placentas. It is characterized by:
a protein proportion essentially consisting of 89±4% of α-amino-acids,
a carbohydrate proportion of 11.1±2.2%, among it 5.3±1.1% of hexoses, 2.8±0.5 of N-acetylated hexosamine, and 2.9±0.6% of N-acetylated neuraminic acid;
a sedimentation coefficient S20x of 3.2±0.3 S;
a molecular weight of 32,000±6,000;
an iso-electric point at pH 4.3±0.3;
an extinction coefficient E1 cm1% (280 nm) of 13.8±1.0;
an electrophoretic mobility in the range between that of albumin and the α1 -globulins;
a specific immunologic reaction with an antibody specifically directed against the protein, and
a proteolytic activity.
Owing to its, normally, very low concentration in human serum, it can be designated as being a trace protein.
The following explanations are given in order to clarify the characteristics of the glycoprotein:
Determination of the sedimentation coefficient was effected in an ultra-centrifuge of Messrs. Beckman (Spinco-Apparatus, type E) designed for analytical investigations, at 6000 revolutions per minute (rpm) in double sector cells with the aid of ultraviolet scanner technique at 280 nm. The solvent was a 0.05 M phosphate buffer (pH 6.8) which contained 0.2 mole/1 of NaCl. The protein concentration was 0.1%. The sedimentation coefficients were calculated on the basis of water at 20° C.
In order to determine the molecular weight, the method of the equilibrium of the sedimentation and electrophoresis in polyacrylamide gel were used. Determination in the ultracentrifuge was carried out at 9,000 rpm. The evaluation was made on the basis of a partial specific volume of 0.74 ml/g. In the ultracentrifuge, a molecular weight of 28,100±2,000 was obtained.
For the electrophoresis in polyacrylamide gel, two methods were used. Separation in normal polyacrylamide (PAA)-gel was carried out according to the method of Zwisler and Biel, Z.klin. Chem. 4, page 58 (1966). For investigation in sodium dodecylsulfate-containing gel, a gel containing 7.5% of PAA and otherwise 0.1% of sodium dodecyl sulfate (SDS) was used. For reduction, the proteins were incubated in 1% of SDS with mercaptoethanol. The proteins were dyed with amido black. Upon migration within the SDS-containing PAA-gel, a molecular weight of 35,000±3,000 was derived for the glycoprotein.
The determination of the iso-electric point was effected with a column (440 ml) of Messrs. LKB Stockholm. The so-called Ampholine mixture had, at the time of the investigation of the glycoprotein, a pH-value of from 3 to 5.
The test for the electrophoretic mobility was effected according to the micro-modification of Beckman Instruments on cellulose acetate foils with a sodium diethyl barbiturate buffer of pH 8.6.
Determination of the carbohydrates was carried out according to the method described by H. E. Schultze, R. Schmidtberger, H. Haupt, Biochem. Z. 329, page 490 (1958).
The analysis for amino-acids was carried out according to S. Moore, D. H. Spackmann, W. H. Stein, Anal. Chem. 30, page 1185, (1958), using the liquid chromatograph Multichrom B of Messrs. Beckman. Cystine was determined after oxidation of the proteins with performic acid (S. Moore et al., Anal. Chem. 30, page 1185, (1958)) and subsequent chromatography (S. Moore, J. Biol. Chem. 238, page 235, (1963)) as cysteinic acid. The content of tryptophan was determined by direct photometric measurement according to H. Edelhoch, Biochemistry 6, page 1948, (1967).
The immunologic characterization of the substance was effected in the most simple way according to a known diffusion process in which the antigen, i.e. the glycoprotein, and an antibody directed against the glycoprotein or antiserum which is not enriched with regard to antibodies are allowed to diffuse against each other in a carrier medium, for example agar-agar. If both reaction components meet under favorable conditions, a visible precipitate is formed. With this knowledge, it is clear to an expert, that all immunological techniques for the detection and determination of the new glycoprotein, as well as of the antibodies directed against this glycoprotein, are possible.
A simple and generally sufficiently exact method for the quantitative determination of the glycoprotein in body fluids or in tissue extracts is the so-called Laurell-technique. It is described in Analyt. Biochem. (New York), 15, page 45 (1966).
The proteolytic action of the glycoprotein was proved on fibrin agar-electrophoresis plates (N. Heimburger, G. Schwick, Protides of the Biological Fluids, 9th Coll., Bruegge, page 303 (1961)).
The present invention furthermore provides a process for isolating the above specified glycoprotein, which process is characterized by fractionating body fluids or extracts of organs which contain the glycoprotein, on the basis of the criteria found according to the invention.
The glycoprotein can be precipitated with neutral salts. With ammonium sulfate which is usually employed for such precipitations, the glycoprotein is precipitated at a saturation concentration of the salt of from 30 to 60% in pH-range in proximity of the neutral point.
According to its molecular weight, the glycoprotein can be isolated by measures which are suitable for the separation of substances with molecular weights between 2500 and 40000 . It is of advantage to use for this purpose the methods of the gel-filtration or ultra-filtration.
The glycoprotein is adsorbed on weakly basic ion-exchangers at a neutral or weakly alkaline pH-value. It is of advantage to use a buffer solution which has a relatively low concentration because adsorption can be prevented by increasing the salt concentration or, also, by lowering the pH. On the other hand, knowing this behavior it is possible to adsorb the glycoprotein and then to elute it again by using more highly concentrated salt solutions or buffer solutions with lower pH-value.
It has been found that the new glycoprotein is not precipitated by the water-soluble organic bases of the acridine and quinoline series which are normally used for protein precipitation methods. At the concentrations usually employed in these processes, it remains in the aqueous supernatant. Subsequently, an acridine base, such as 2-ethoxy-6,9-diaminoacridine lactate or a quinoline base such as bis-(2-methyl-4-aminoquinolyl-6-)-carbamide-hydrochloride, can be used for the precipitation of accompanying proteins, the glycoprotein of the invention remaining in the supernatant. Similar considerations may be made when using hydroxyl-apatite as an adsorbant for proteins. The new glycoprotein shows no affinity to hydroxyl-apatite, whereas a series of accompanying proteins are fixed by hydroxyl-apatite. This behavior of the glycoprotein is characteristic, so that it is proposed to designate the glycoprotein as a hydroxyl-apatite-passing globuline (HPG-1).
On the basis of the knowledge of the electrophoretic mobility, preparative zone electrophoresis may be used for the enrichment or isolation of the glycoprotein.
The affinity of the glycoprotein, owing to its immunological behavior, may be used to enrich the glycoprotein with the aid of so-called immuno-adsorption processes. For this purpose, an immuno-adsorbant, i.e., a carrier-bound antibody directed against the new glycoprotein and which is capable of specifically binding the glycoprotein, can be prepared in a known manner. The glycoprotein can subsequently be eluted by modification of the conditions of the medium as already described in the literature.
Isolation of the substance of the present invention can thus be effected by a selected combination of the above-mentioned methods which lead, on the one hand, to an enrichment of the glycoprotein, and, on the other hand, to its separation from other accompanying proteins. Accordingly, the subject of the present invention resides in the individual steps for enriching the new glycoprotein and in the processes for its purification reached by a combination of these measures. The guideline for the process for preparing the glycoprotein consists in isolating in each case that fraction which shows a positive immunological reaction with an antiserum directed against the new glycoprotein.
After having carried out the above described process steps it has been found in some cases that the glycoprotein is still contaminated by other accompanying proteins which are immunologically detectable. Generally, these are trace proteins as the new glycoprotein itself. In this case, the contaminating substances are eliminated by their specific adsorption. For this purpose the usual methods of immuno adsorption are used in which antibodies bound to a carrier and directed against the protein to be eliminated are employed as adsorbants. In many cases, the largely-pure new glycoprotein still contains traces of the pregnancy-specific β1 -glycoprotein and/or the α-1 -B-glycoprotein, which is also designated as easily precipitable α1 -glycoprotein. For separating the last mentioned, immunoglobulins which are directed against the proteins and which are bound covalently on cross-linked agar preparations, for example SEPHAROSE, are used. The protein solution introduced into a column which has been filled with an immuno-adsorbant passes without difficulty through the column since only those components are bound against which the carrier contains an immunologically active partner. The new glycoprotein can be freed from inpurities in this manner.
For preparing the new glycoprotein, several of the measures indicated are combined with each other and only that fraction is further treated in which the new glycoprotein can be proved immunologically, whereas the remaining fractions are rejected.
As starting material for the isolation of the new glycoprotein, any body fluid or every organ extract may be used in which the glycoprotein can be proved immunologically. It is preferred to use extracts of human placentas which are obtained by comminution and extraction with water or a dilute, suitably a less than 10% strength, salt solution, advantageously with a 0.5 % strength liquid salt solution, for example sodium chloride. Suitably, about 1-5 liters of the extracted solution are used for 1 kg of placentas. The components which have not dissolved are separated from the extract by centrifugation or filtration.
The process for enriching is characterized by that at least one of the following process steps is applied to body fluids which contain the new glycoprotein and the fraction containing the glycoprotein in enriched form is isolated.
(a) Addition of water-soluble derivatives of an acridine or quinoline base, preferably 2-ethoxy-6,9-diaminoacridine-lactate in the pH-range of from 5-10, preferably at about pH 8, up to a final concentration of about 0,8% (weight to volume) the glycoprotein remaining essentially in the supernatant.
(b) Addition of neutral salts until precipitation of the glycoprotein, preferably ammonium sulfate at an about neutral pH-value of from 5-8, up to 30 to 60% of the saturation concentration of the ammonium sulfate.
(c) Adsorption of the glycoprotein on a weakly basic ion-exchanger exchanger such as diethylaminoethyl-cellulose, at a conductivity of the solution of 0-2 mS and at a neutral or weakly alkaline pH-value (6-9), for example using an about 0,01 M buffer having a pH-value of about 8. A buffer which is preferably used is, for example, tris-hydroxymethylamino-methane-HCl. Elution of the glycoprotein can be effected by lowering the pH-value below pH 7.0 or by increasing the conductivity to more than 5 mS.
(d) Separation on the basis of the size of the molecules (molecular sieve fractionation). Gel filtration in a column which is filled with a polymer of a corresponding pore size, for example epichlorohydrin-cross-linked dextran such as SEPHADEX® produced by Messrs. Pharmacia, Uppsala, with the aim of enriching proteins with a molecular weight of about 50,000 is particularly suitable. But products such as ULTROGEL® by Messrs. LKB, Bromma or BIO-GEL P® produced by Bio-Rad Laboratories, Richmond, California, may likewise be used.
(e) Adsorption with hydroxyl-apatite. Since the glycoprotein is not bound by hydroxyl-apatite in dilute phosphate buffer solution, hydroxyl-apatite represents a suitable agent for removing accompanying proteins of glycoprotein from the solution. The protein solution is suitably adjusted to a pH-value around the neutral point and the conductivity of the solution is kept to about 1 mS.
(f) Preparative zone electrophoresis For carrying out an electrophoresis, a solution which contains the glycoprotein, preferably an alkaline buffer solution, for example in a sodium diethylbarbiturate buffer of pH 8.6 and anion strength of 0.1, is suitable. The solution is introduced into an apparatus for preparative electrophoresis, for example that described by N. Heimburger and R. Schmidtberger in Behringwerke Mitteilungen, Volume 43, page 83 et seq., in particular on pages 119-120. This carrier electrophoresis apparatus is horizontally arranged in an open trough, in which the carrier material is cooled to below 10° C. in order to withdraw the Joule's heat which is formed during the electrophoresis. As the carrier material, substances which are inert toward proteins, preferably polyvinyl chloride or its copolymers in the form of a fine granulate, are used.
It is recommended to carry out the electrophoresis in the alkaline pH-range, preferably at about pH 8.6, at an ion strength of 0.08-0.12 and a field strength of 4-6 volts/cm. When using 0.1 M sodium diethylbarbiturate buffer having a pH-value of 8.6, the glycoprotein migrates in an electrical field in the range of the plasmaproteins between albumin and the α1 -globulins.
For isolating the new glycoprotein, a corresponding zone is cut out and eluted with water or aqueous salt solutions, for example an 0.5 to 1% strength sodium chloride solution, from the inert carrier material.
The protein prepared according to the invention has antigenic properties. When immunizing animals with it according to known methods, specific antibodies are formed in the blood of the immunized animals. Their sera can be isolated according to the usual methods and the antibodies contained in them can be enriched. The antisera may be used in known immunological processes for the proof and determination of the new protein in body fluids, in particular in the blood serum. In addition, the glycoprotein has proteolytic and fibrinolytic properties. A thrombolytic action and a disaggregating effect on blood platetets was likewise proved. Accordingly, the glycoprotein of the invention has properties of a valuable medicament. The following Example illustrates the invention.
150 kg of deep frozen placentas were comminuted and extracted with 150 l of a 0.5% strength aqueous sodium chloride solution. The extract was adjusted to pH 8 with 2 N-sodium hydroxide and combined with 50 l of a 3% strength aqueous solution of diaminoethoxyacridine lactate. After a dwelling time of 1 hour, the supernatant which contained the glycoprotein of the invention (HPG-1) was decanted off, combined with 5% to solid sodium chloride (11 kg) for separating the diaminoethoxyacridine lactate which still remained in solution, filtered and combined with 30%--referred to the weight of the liquid--of solid ammonium sulfate and stirred well. After 1 hour, the precipitate was filtered off.
500 g of the precipitate deposited on the filter were dissolved in 500 ml of distilled water and dialyzed against a 0.01 molar tris-(oxymethyl)-aminomethane-HCl buffer solution of a pH-value of 7.0 and which contained 0.05% sodium azide. The dialyzed solution was centrifuged and the supernatant was filled up with the same buffer solution to a volume of 2000 ml, adjusted to pH 8.0 with 0.1 N-sodiumhydroxide solution and stirred with 500 g of wet diethylaminoethyl cellulose (Messrs. SERVA, Heidelberg) for 1 hour.
The diethylaminoethyl cellulose was then separated from the solution by filtration, washed twice with 1 liter portions of 0.01 molar tris-(oxymethyl)-aminomethane-HCl buffer having a pH-value of 8.0 and eluted three times with 500 ml portions of 0.02 molar tris-(oxymethyl)-aminomethane-HCl buffer, pH 6.5, which contained 0.85% of sodium chloride and 0.05% of sodium azide.
The combined eluates were combined with 30% of ammonium sulfate, referred to the weight of the liquid, and the whole was stirred. The precipitate, which contained the glycoprotein (HPG-1), was dissolved in 300 ml of distilled water. The protein solution was dialyzed against tris-hydroxymethyl-aminomethane-HCl buffer of pH 8.0 which contained 1.0 mole of sodium chloride/liter and introduced into a column (100×20 cm) filled with SEPHADEX G-150 and eluted with the mentioned buffer. During the elution, a fractionation of the proteins according to their molecular size took place.
The eluates were subsequently tested with specific antiserum, the fractions containing the glycoprotein (HPG-1) were collected and the proteins were precipitated therefrom as described above with 30% of solid ammonium sulfate.
For further purification, the precipitate was dissolved in 50 ml of water, dialyzed against a 0.005 M phosphate buffer, pH 6.8, and introduced into a column filled with hydroxyl-apatite (size of the column 3×23 cm). Development of the column was effected with the 0.005 M phosphate buffer, pH 6.8. The glycoprotein (HPG-1) appeared in the eluate. The eluate itself was concentrated on an ultrafilter. The concentrate was then dialyzed against a 0.01 M tris-HCl-buffer, pH 7.0 and adsorbed on DEAE-SEPHADEX (column 3×23 cm). For eluting and separating the adsorbed proteins, a NaCl-gradient of 0-2% was used. The eluate fractions which contained the glycoprotein (HPG-1) were collected, concentrated, dialyzed against water and then lyophilized. About 20 mg of the new glycoprotein having a purity of >99% were obtained.
It showed the following amino-acid composition (frequency with variation coefficient (VC) in %):
______________________________________ Frequency in Mole % VC %______________________________________Lysine 0.67 39.25Histidine 3.36 9.97Arginine 5.09 6.58Aspartic acid 5.87 3.07Threonine 3.83 15.74Serine 7.84 8.22Glutamic acid 11.76 1.35Proline 6.46 1.71Glycine 10.24 4.49Alanine 11.25 3.76Cystine/2 5.10 6.34Valine 6.87 5.18Methionine 0.42 14.48Isoleucine 2.47 5.07Leucine 11.42 4.23Tyrosine 2.95 5.45Phenylalanine 2.42 13.52Tryptophan 1.98 11.37______________________________________
Patent Citations
Referenced by
Legal Events
Original Image