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Recognition of double helical DNA by alternate strand triple helix formation
SciTech Connect (OSTI)
The triplet specificities and required strand orientations of two classes of DNA triple helices can be combined to target double helical sequences containing all four base pairs by alternate strand triple helix formation. This allows for the use of oligonucleotides containing only natural 3{prime}-5{prime} phosphodiester linkages to simultaneously bind both strands of double helical DNA in the major groove. The stabilities and structures of these alternate strand triple helices depend on whether the binding site sequences is 5{prime}-(purine){sub m}(pyrimidine){sub n}-3{prime} or 5{prime}-(pyrimidine){sub m}(purine){sub n}-3{prime}. The sequence type 5{prime}-(purine){sub m}- (pyrimidine){sub n}-3{prime} was targeted with an oligonucleotide consisting of a pyrimidine domain and a purine domain linked by a 3{prime}-5{prime} phosphodiester. To bind the duplex sequence type 5{prime}-(pyrimidine){sub m}(purine){sub n}-3{prime}, the third strand requires at least two nucleotides linking binding domains at the site of crossover in the major groove. A new class of oligonucleotides capable of binding mixed duplex sequences by crossovers in the major groove may be possible. 15 refs., 13 figs.
Beal, P.A.; Dervan, P.B. [California Institute of Technology, Pasadena, CA (United States)
Site-Selective Binding of Nanoparticles to Double-Stranded DNA via Peptide Nucleic Acid "Invasion"
We demonstrate a novel method for by-design placement of nano-objects along double-stranded (ds) DNA. A molecular intercalator, designed as a peptide nucleic acid (PNA)-DNA chimera, is able to invade dsDNA at the PNA-side due to the hybridization specificity between PNA and one of the duplex strands. At the same time, the single-stranded (ss) DNA tail of the chimera, allows for anchoring of nano-objects that have been functionalized with complementary ssDNA. The developed method is applied for interparticle attachment and for the fabrication of particle clusters using a dsDNA template. This method significantly broadens the molecular toolbox for constructing nanoscale systems by including the most conventional not yet utilized DNA motif, double helix DNA.
Stadler, A.L.; van der Lelie, D.; Sun, D.; Maye, M. M.; Gang, O.
Protein Bridges DNA Base and Nucleotide Excision Repair Pathways
Broader source: All U.S. Department of Energy (DOE) Office Webpages (Extended Search)
a nucleotide from the DNA double helix to its active site to access damaged nucleotides. But unlike AGT and most other known DNA nucleotide-flipping proteins, this...
Spin transport and spin polarization properties in double-stranded DNA
We study the spin-dependent electron transport through a double-stranded DNA (dsDNA) using the Bogoliubov-de Gennes equations and non-equilibrium Green's function method. We calculate the spin-dependent electron conductance and spin-polarization for different lengths, helix angles, twist angles of dsDNA, the environment-induced dephasing factors, and hopping integral. It is shown that the conductance decreases by increasing the length and dephasing factor. Also, we show that the spin-polarization depends on the helical symmetry and the length of DNA. It is shown that the double-stranded DNA can act as a perfect spin filter. Finally, we show that the sign of spin polarization can be inverted from +1 (?1) to ?1 (+1) for some values of hopping integral.
Simchi, Hamidreza, E-mail: email@example.com [Department of Physics, Iran University of Science and Technology, Narrmak, Tehran 16844 (Iran, Islamic Republic of); Semiconductor Technology Center, Tehran (Iran, Islamic Republic of); Esmaeilzadeh, Mahdi, E-mail: firstname.lastname@example.com; Mazidabadi, Hossein [Department of Physics, Iran University of Science and Technology, Narrmak, Tehran 16844 (Iran, Islamic Republic of)
Structure of DNA-Bound FEN1 Reveals Mechanism of Action
double helix. A protein called DNA polymerase does most of this work, adding nucleotides complementary to the template's nucleotides. But before polymerase builds the...
Probing Protein-DNA Interactions by Unzipping a Single DNA Double Helix
E-Print Network [OSTI]
., 2000; Bianco et al., 2001; Dohoney and Gelles, 2001; Strick et al., 2000), as well as the investigation
Wang, Michelle
Helix coupling
DOE Patents [OSTI]
A coupling for connecting helix members in series, which consists of a pair of U-shaped elements, one of which is attached to each helix end with the "U" sections of the elements interlocked. The coupling is particularly beneficial for interconnecting helical Nitinol elements utilized in thermal actuators or engines. Each coupling half is attached to the associated helix at two points, thereby providing axial load while being easily removed from the helix, and reusable.
Ginell, W.S.
A coupling for connecting helix members in series, which consists of a pair of U-shaped elements, one of which is attached to each helix end with the U sections of the elements interlocked. The coupling is particularly beneficial for interconnecting helical Nitinol elements utilized in thermal actuators or engines. Each coupling half is attached to the associated helix at two points, thereby providing axial load while being easily removed from the helix, and reusable.
DNA purification by triplex-affinity capture and affinity capture electrophoresis
The invention provides a method for purifying or isolating double stranded DNA intact using triple helix formation. The method includes the steps of complexing an oligonucleotide and double stranded DNA to generate a triple helix and immobilization of the triple helix on a solid phase by means of a molecular recognition system such as avidin/biotin. The purified DNA is then recovered intact by treating the solid phase with a reagent that breaks the bonds between the oligonucleotide and the intact double stranded DNA while not affecting the Watson-Crick base pairs of the double helix. The present invention also provides a method for purifying or isolating double stranded DNA intact by complexing the double stranded DNA with a specific binding partner and recovering the complex during electrophoresis by immobilizing it on a solid phase trap imbedded in an electrophoretic gel. 6 figs.
Cantor, C.R.; Ito, Takashi; Smith, C.L.
SOLVING LARGE DOUBLE DIGESTION PROBLEMS FOR DNA RESTRICTION MAPPING BY USING BRANCH;Solving Large Double Digestion Problems for DNA Restriction Mapping by Using Branch-and-Bound Integer.S.A. Abstract. The double digestion problem for DNA restriction mapping has been proved to be NP
Helical Disruptions in Small Loops of DNA
The thermodynamical stability of DNA minicircles is investigated by means of path integral techniques. Hydrogen bonds between base pairs on complementary strands can be broken by thermal fluctuations and temporary fluctuational openings along the double helix are essential to biological functions such as transcription and replication of the genetic information. Helix unwinding and bubble formation patterns are computed in circular sequences with variable radius in order to analyze the interplay between molecule size and appearance of helical disruptions. The latter are found in minicircles with $100$ base pairs and appear as a strategy to soften the stress due to the bending and torsion of the helix.
Zoli, Marco
Mechanistic studies of bleomycin-mediated double-stranded DNA cleavage and structural studies of DNA containing normal and 4'-oxidized abasic sites
In order to examine the role of partial intercalation in double-stranded (ds) DNA cleavage mediated by a single bleomycin (BLM), a bulky group ([-cyclodextrin) was chemically attached to the polyamine tail of BLM A5 to ...
Chen, Jingyang, Ph. D. Massachusetts Institute of Technology
Probability of double-strand breaks in genome-sized DNA by {gamma}-ray decreases markedly as the DNA concentration increases
By use of the single-molecule observation, we count the number of DNA double-strand breaks caused by {gamma}-ray irradiation with genome-sized DNA molecules (166 kbp). We find that P{sub 1}, the number of double-strand breaks (DSBs) per base pair per unit Gy, is nearly inversely proportional to the DNA concentration above a certain threshold DNA concentration. The inverse relationship implies that the total number of DSBs remains essentially constant. We give a theoretical interpretation of our experimental results in terms of attack of reactive species upon DNA molecules, indicating the significance of the characteristics of genome-sized giant DNA as semiflexible polymers for the efficiency of DSBs.
Shimobayashi, Shunsuke F. [Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502 (Japan); Iwaki, Takafumi [Fukui Institute for Fundamental Chemistry, Kyoto University, Kyoto 606-8103 (Japan); Mori, Toshiaki [Radiation Research Center, Osaka Prefecture University, Sakai 599-8570 (Japan); Yoshikawa, Kenichi [Department of Physics, Graduate School of Science, Kyoto University, Kyoto 606-8502 (Japan); Faculty of Life and Medical Sciences, Doshisha University, Kyoto 610-0394 (Japan)
Processing of 3'-Phosphoglycolate-Terminated DNA Double-StrandBreaks by Artemis Nuclease
The Artemis nuclease is required for V(D)J recombination and for repair of an as yet undefined subset of radiation-induced DNA double-strand breaks. To assess the possibility that Artemis functions on oxidatively modified double-strand break termini, its activity toward model DNA substrates, bearing either 3{prime}-hydroxyl or 3{prime}-phosphoglycolate moieties, was examined. A 3{prime}-phosphoglycolate had little effect on Artemis-mediated trimming of long 3{prime} overhangs (>9 nucleotides), which were efficiently trimmed to 4-5 nucleotides. However, 3{prime}-phosphoglycolates on overhangs of 4-5 bases promoted selective Artemis-mediated trimming of a single 3{prime}-terminal nucleotide, while at least 2 nucleotides were trimmed from identical hydroxyl-terminated substrates. Artemis also efficiently removed a single nucleotide from a phosphoglycolate-terminated 3-base 3{prime} overhang, while leaving an analogous hydroxyl-terminated overhang largely intact. Such removal was dependent upon Ku, DNA-dependent protein kinase, and ATP. Together, these data suggest that Artemis-mediated cleavage of 3{prime} overhangs requires a minimum of 2 nucleotides, or a nucleotide plus a phosphoglycolate, 3{prime} to the cleavage site. Shorter 3{prime}-phosphoglycolate-terminated overhangs and blunt ends were also processed by Artemis, but much less efficiently. Consistent with the in vitro substrate specificity of Artemis, human cells lacking Artemis exhibited hypersensitivity to X-rays, bleomycin and neocarzinostatin, which all induce 3{prime}-phosphoglycolate-terminated double-strand breaks. Collectively, these results suggest that 3{prime}-phosphoglycolate termini and/or specific classes of DNA ends that arise from such blocked termini are relevant Artemis substrates in vivo.
Povrik, Lawrence F.; Zhou, Tong; Zhou, Ruizhe; Cowan, Morton J.; Yannone, Steven M.
Coordinateendonucleolytic 5' and 3' trimming of terminally blocked blunt DNA double-strand break ends by Artemis nuclease and DNA-dependent protein kinase
Previous work showed that, in the presence of DNA-PK, Artemis slowly trims 3'-phosphoglycolate-terminated blunt ends. To examine the trimming reaction in more detail, long internally labeled DNA substrates were treated with Artemis. In the absence of DNA-PK, Artemis catalyzed extensive 5' {yields} 3' exonucleolytic resection of double-stranded DNA. This resection required a 5'-phosphate but did not require ATP, and was accompanied by endonucleolytic cleavage of the resulting 3' overhang. In the presence of DNA-PK, Artemis-mediated trimming was more limited, was ATP-dependent, and did not require a 5'-phosphate. For a blunt end with either a 3'-phosphoglycolate or 3'-hydroxyl terminus, endonucleolytic trimming of 2-4 nucleotides from the 3'-terminal strand was accompanied by trimming of 6 nucleotides from the 5'-terminal strand. The results suggest that autophosphorylated DNA-PK suppresses the exonuclease activity of Artemis toward blunt-ended DNA, and promotes slow and limited endonucleolytic trimming of the 5'-terminal strand, resulting in short 3' overhangs that are trimmed endonucleolytically. Thus, Artemis and DNA-PK can convert terminally blocked DNA ends of diverse geometry and chemical structure to a form suitable for polymerase mediated patching and ligation, with minimal loss of terminal sequence. Such processing could account for the very small deletions often found at DNA double-strand break repair sites.
Povirk, Lawrence; Yannone, Steven M.; Khan, Imran S.; Zhou, Rui-Zhe; Zhou, Tong; Valerie, Kristoffer; F., Lawrence
Proximity-induced superconductivity effect in a double-stranded DNA
We study the proximity-induced superconductivity effect in a double-stranded DNA by solving the Bogoliubov-de Gennes equations and taking into account the effect of thermal fluctuations of the twist angle between neighboring base pairs. We show that the electron conductance is spin-dependent and the conductance of spin up (down) increases (decreases) due to the spin-orbit coupling (SOC). It is found that, for T?100?K, the band gap energy is temperature-independent and it decreases due to the SOC. In addition, by solving the Bogoliubov-de Gennes equations and local gap parameter equation self-consistently, we find the critical temperature at which transition to superconductivity can take place.
Detection and Repair of Ionizing Radiation-Induced DNA Double Strand Breaks: New Developments in Nonhomologous End Joining
DNA damage can occur as a result of endogenous metabolic reactions and replication stress or from exogenous sources such as radiation therapy and chemotherapy. DNA double strand breaks are the most cytotoxic form of DNA damage, and defects in their repair can result in genome instability, a hallmark of cancer. The major pathway for the repair of ionizing radiation-induced DSBs in human cells is nonhomologous end joining. Here we review recent advances on the mechanism of nonhomologous end joining, as well as new findings on its component proteins and regulation.
Wang, Chen [Departments of Biochemistry and Molecular Biology and Oncology, and Southern Alberta Cancer Research Institute, University of Calgary, Calgary (Canada)] [Departments of Biochemistry and Molecular Biology and Oncology, and Southern Alberta Cancer Research Institute, University of Calgary, Calgary (Canada); Lees-Miller, Susan P., E-mail: email@example.com [Departments of Biochemistry and Molecular Biology and Oncology, and Southern Alberta Cancer Research Institute, University of Calgary, Calgary (Canada)
Telomeres and DNA double-strand breaks: ever the twain shall S. M. Baileya,* and M. N. Cornforthb
between the naturally occurring DNA double-strand breaks (DSBs) found at chromosome ends (i.e., telomeres Collins, CO 80523-1618 (USA), Fax: +19704917742, e-mail: firstname.lastname@example.com b Department of Radiation on their inability to rejoin with broken chromosome ends produced by radiation. Today, we recognize two essential
Rapid purification of circular DNA by triplex-mediated affinity capture
A single-step capture of a target supercoiled double-stranded DNA molecule is accomplished by forming a local triple-helix among two strands of the supercoiled circular DNA and an oligonucleotide probe. The oligonucleotide is bound to an immobilizing support which facilitates the immobilization and purification of target DNA molecules. Non-target DNA molecules and other contaminating cellular material are easily removed by washing. The triple-helical structure is destabilized by raising the pH, leaving purified target DNA in the supernatant and reusable affinity capture oligonucleotide secured to the immobilizing support. 3 figs.
Ji, H.; Smith, L.M.
C-H..O Hydrogen Bonds in Minor Groove of A-tracts in DNA Double Helices
C-H..O Hydrogen Bonds in Minor Groove of A-tracts in DNA Double Helices Anirban Ghosh and Manju-pair as well as cross-strand C-H..O hydrogen bonds in the minor groove. The C2-H2..O2 hydrogen bonds within leads to a narrow minor groove in these regions. # 1999 Academic Press Keywords: C-H..O hydrogen bonds
Bansal, Manju
Intense photoluminescence from dried double-stranded DNA and single-walled carbon nanotube hybrid
Semiconducting single-walled carbon nanotubes (SWNTs) show near-infrared photoluminescence (PL) when they are individually isolated. This was an obstacle to use photonic properties of SWNTs on a solid surface. We show that SWNTs wrapped with DNA maintain intense PL under the dry conditions. SWNTs are well isolated individually by DNA even when the DNA-SWNT hybrids are agglomerated. This finding opens up application of SWNTs to photonic devices.
Ito, M.; Kobayashi, T.; Ito, Y.; Hayashida, T.; Nii, D.; Umemura, K.; Homma, Y. [Department of Physics, Tokyo University of Science, Tokyo 162-8601 (Japan)
Dyes designed for high sensitivity detection of double-stranded DNA
Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a cationic chain. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.
Glazer, Alexander N. (Orinda, CA); Benson, Scott C. (Oakland, CA)
Novel fluorescent dyes are provided, characterized by having a fluorophore joined to a polycationic chain of at least two positive charges. The dyes are found to provide for high enhancement upon binding to nucleic acid and have strong binding affinities to the nucleic acid, as compared to the fluorophore without the polycationic chain. The dyes find use in detection of dsDNA in gel electrophoresis and solution at substantially higher sensitivities using substantially less dye.
Glazer, Alexander N. (Orinda, CA); Benson, Scott C. (Albany, CA)
Absence of zero-temperature transmission rate of a double-chain tight-binding model for DNA with random sequence of nucleotides in thermodynamic limit
The zero-temperature transmission rate spectrum of a double-chain tight-binding model for real DNA is calculated. It is shown that a band of extended-like states exists only for finite chain length with strong inter-chain coupling. While the whole spectrum tends to zero in thermodynamic limit, regardless of the strength of inter-chain coupling. It is also shown that a more faithful model for real DNA with periodic sugar-phosphate chains in backbone structures can be mapped into the above simple double-chain tight-binding model. Combined with above results, the transmission rate of real DNA with long random sequence of nucleotides is expected to be poor.
Gang Xiong; X. R. Wang
September 15, 2014 Computational analysis key to structural understanding of molecular machine that targets viral DNA LOS ALAMOS, N.M., Sept. 15, 2014-When this week's print issue...
Analysis of the structural changes caused by positive DNA supercoiling
The procession of helix-tracking enzymes along a DNA molecule results in the formation of supercoils in the DNA, with positive supercoiling (overwinding) generated ahead of the enzyme, and negative supercoiling (underwinding) ...
Barth, Marita Christine
Investigating Deinococcus radiodurans RecA Protein Filament Formation on Double-Stranded DNA by a Real-Time
to desiccation.2 Many hypotheses have been proposed to explain the high- efficiency repair, including a ringlike condensed chromosome structure that could restrict fragment DNA diffusion,3-5 a high Mn2+ concentration: With the aid of an efficient, precise, and almost error-free DNA repair system, Deinococcus radiodurans can
Cox, Michael M.
Energetics of [alpha]-helix formation in peptides and proteins
This thesis focuses on the energetics of !-helix formation in peptides and proteins. The [alpha]-helix is the most prevalent type of secondary structure found in proteins, and has arguably dominated our thinking about ...
Schubert, Christian Reinhold
Grooving Down the Helix http://www.bnl.gov/bnlweb/pubaf/pr/PR_display.asp?prID=1038&template=Today[12/7/2009 9:39:49 AM
the Helix - 12/4/2009 Brookhaven Hosts ATLAS Jamboree in Anticipation of LHC Second Run - 12 move in a straight line parallel to the DNA axis or trace more complex helical paths, following
Heller, Eric
PAXX, a paralog of XRCC4 and XLF, interacts with Ku to promote DNA double-strand break repair
regions of PAXX1-204 and PAXXV199A/F201A were amplified from the vectors by PCR and cloned into the pcDNA3.1(-) vector (a gift from Dr. V. Bolanos-Garcia) together with GFP or FLAG tags using In-Fusion (Clontech). Vectors were sequenced by the DNA... -treated or treated for 1 hour with 300 �M phleomycin to induce large numbers of DSBs, before being harvested either at this point or after an additional 1-hour recovery period in phleomycin-free medium after three PBS washes. Cells were washed twice in ice-cold PBS...
Ochi, Takashi; Blackford, Andrew N.; Coates, Julia; Jhujh, Satpal; Mehmood, Shahid; Tamura, Naoka; Travers, Jon; Wu, Qian; Draviam, Viji M.; Robinson, Carol V.; Blundell, Tom L.; Jackson, Stephen P.
Double stranded nucleic acid biochips
This invention describes a new method of constructing double-stranded DNA (dsDNA) microarrays based on the use of pre-synthesized or natural DNA duplexes without a stem-loop structure. The complementary oligonucleotide chains are bonded together by a novel connector that includes a linker for immobilization on a matrix. A non-enzymatic method for synthesizing double-stranded nucleic acids with this novel connector enables the construction of inexpensive and robust dsDNA/dsRNA microarrays. DNA-DNA and DNA-protein interactions are investigated using the microarrays.
Chernov, Boris; Golova, Julia
Homologous recombination contributes to the repair of DNA double-strand breaks induced by high-energy iron ions
To test the contribution of homologous recombinational repair (HRR) in repairing DNA damaged sites induced by high-energy iron ions, we used: (1) HRR-deficient rodent cells carrying a deletion in the RAD51D gene and (2) syngeneic human cells impaired for HRR by RAD51D or RAD51 knockdown using RNA interference. We show that in response to iron ions, HRR contributes to cell survival in rodent cells, and that HRR-deficiency abrogates RAD51 foci formation. Complementation of the HRR defect by human RAD51D rescues both enhanced cytotoxicity and RAD51 foci formation. For human cells irradiated with iron ions, cell survival is decreased, and, in p53 mutant cells, the levels of mutagenesis are increased when HRR is impaired. Human cells synchronized in S phase exhibit more pronounced resistance to iron ions as compared with cells in G1 phase, and this increase in radioresistance is diminished by RAD51 knockdown. These results implicate a role for RAD51-mediated DNA repair (i.e. HRR) in removing a fraction of clustered lesions induced by charged particle irradiation. Our results are the first to directly show the requirement for an intact HRR pathway in human cells in ensuring DNA repair and cell survival in response to high-energy high LET radiation.
Zafar, Faria; Seidler, Sara B.; Kronenberg, Amy; Schild, David; Wiese, Claudia
Supporting Information Formation and Properties of Selenium Double-Helices
-Wall Carbon Nanotubes: Experiment and Theory Toshihiko Fujimori, Renato Batista dos Santos,,§ Takuya Hayashi-helix structures with long unit cells undergo a small axial offset. #12;S3 Figure S3. Simulated TEM images of Se,5) carbon nanotube. (c) Se double-helix with a pitch length of 0.5 nm inside a (5,5) carbon nanotube. (d) Se
Tománek, David
Free energy landscape and characteristic forces for the initiation of DNA unzipping
DNA unzipping, the separation of its double helix into single strands, is crucial in modulating a host of genetic processes. Although the large-scale separation of double-stranded DNA has been studied with a variety of theoretical and experimental techniques, the minute details of the very first steps of unzipping are still unclear. Here, we use atomistic molecular dynamics (MD) simulations, coarse-grained simulations and a statistical-mechanical model to study the initiation of DNA unzipping by an external force. The calculation of the potential of mean force profiles for the initial separation of the first few terminal base pairs in a DNA oligomer reveal that forces ranging between 130 and 230 pN are needed to disrupt the first base pair, values of an order of magnitude larger than those needed to disrupt base pairs in partially unzipped DNA. The force peak has an "echo," of approximately 50 pN, at the distance that unzips the second base pair. We show that the high peak needed to initiate unzipping derives...
Mentes, Ahmet; Brunk, Elizabeth; Wereszczynski, Jeff; Joyeux, Marc; Andricioaei, Ioan
DNA unzipping, the separation of its double helix into single strands, is crucial in modulating a host of genetic processes. Although the large-scale separation of double-stranded DNA has been studied with a variety of theoretical and experimental techniques, the minute details of the very first steps of unzipping are still unclear. Here, we use atomistic molecular dynamics (MD) simulations, coarse-grained simulations and a statistical-mechanical model to study the initiation of DNA unzipping by an external force. The calculation of the potential of mean force profiles for the initial separation of the first few terminal base pairs in a DNA oligomer reveal that forces ranging between 130 and 230 pN are needed to disrupt the first base pair, values of an order of magnitude larger than those needed to disrupt base pairs in partially unzipped DNA. The force peak has an "echo," of approximately 50 pN, at the distance that unzips the second base pair. We show that the high peak needed to initiate unzipping derives from a free energy basin that is distinct from the basins of subsequent base pairs because of entropic contributions and we highlight the microscopic origin of the peak. Our results suggest a new window of exploration for single molecule experiments.
Ahmet Mentes; Ana Maria Florescu; Elizabeth Brunk; Jeff Wereszczynski; Marc Joyeux; Ioan Andricioaei
E-Print Network 3.0 - a-helix peptide fragment Sample Search...
the 310... with the states being a-helix (mauve), 310-helix (red), turn regions (cyan), coil (white). doi:10.1371journal... ) Transformation of an alpha-helix peptide into a...
Pliable DNA Conformation of Response Elements Bound to Transcription Factor p63
We show that changes in the nucleotide sequence alter the DNA conformation in the crystal structures of p63 DNA-binding domain (p63DBD) bound to its response element. The conformation of a 22-bp canonical response element containing an AT spacer between the two half-sites is unaltered compared with that containing a TA spacer, exhibiting superhelical trajectory. In contrast, a GC spacers abolishes the DNA superhelical trajectory and exhibits less bent DNA, suggesting that increased GC content accompanies increased double helix rigidity. A 19-bp DNA, representing an AT-rich response element with overlapping half-sites, maintains superhelical trajectory and reveals two interacting p63DBD dimers crossing one another at 120{sup o}. p63DBD binding assays to response elements of increasing length complement the structural studies. We propose that DNA deformation may affect promoter activity, that the ability of p63DBD to bind to superhelical DNA suggests that it is capable of binding to nucleosomes, and that overlapping response elements may provide a mechanism to distinguish between p63 and p53 promoters.
Chen, Chen; Gorlatova, Natalia; Herzberg, Osnat (Maryland)
Replication protein A and g-H2AX foci assembly is triggered by cellular response to DNA double-strand breaks
pathway that removes the oxidized base lesions from the genomic DNA [6]. Evidence for the stimulation
Induction and Rejoining of DNA Double Strand Breaks Assessed by H2AX Phosphorylation in Melanoma Cells Irradiated with Proton and Lithium Beams
Purpose: The aim of this study was to evaluate the induction and rejoining of DNA double strand breaks (DSBs) in melanoma cells exposed to low and high linear energy transfer (LET) radiation. Methods and Materials: DSBs and survival were determined as a function of dose in melanoma cells (B16-F0) irradiated with monoenergetic proton and lithium beams and with a gamma source. Survival curves were obtained by clonogenic assay and fitted to the linear-quadratic model. DSBs were evaluated by the detection of phosphorylated histone H2AX ({gamma}H2AX) foci at 30 min and 6 h post-irradiation. Results: Survival curves showed the increasing effectiveness of radiation as a function of LET. {gamma}H2AX labeling showed an increase in the number of foci vs. dose for all the radiations evaluated. A decrease in the number of foci was found at 6 h post-irradiation for low LET radiation, revealing the repair capacity of DSBs. An increase in the size of {gamma}H2AX foci in cells irradiated with lithium beams was found, as compared with gamma and proton irradiations, which could be attributed to the clusters of DSBs induced by high LET radiation. Foci size increased at 6 h post-irradiation for lithium and proton irradiations in relation with persistent DSBs, showing a correlation with surviving fraction. Conclusions: Our results showed the response of B16-F0 cells to charged particle beams evaluated by the detection of {gamma}H2AX foci. We conclude that {gamma}H2AX foci size is an accurate parameter to correlate the rejoining of DSBs induced by different LET radiations and radiosensitivity.
Ibanez, Irene L. [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Bracalente, Candelaria [Comision Nacional de Energia Atomica, San Martin (Argentina); Molinari, Beatriz L. [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Palmieri, Monica A. [Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (Argentina); Policastro, Lucia [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Kreiner, Andres J. [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Escuela de Ciencia y Tecnologia, Universidad Nacional de San Martin (Argentina); Burlon, Alejandro A. [Comision Nacional de Energia Atomica, San Martin (Argentina); Escuela de Ciencia y Tecnologia, Universidad Nacional de San Martin (Argentina); Valda, Alejandro [Escuela de Ciencia y Tecnologia, Universidad Nacional de San Martin (Argentina); Navalesi, Daniela [Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (Argentina); Davidson, Jorge; Davidson, Miguel [Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Vazquez, Monica; Ozafran, Mabel [Comision Nacional de Energia Atomica, San Martin (Argentina); Duran, Hebe [Comision Nacional de Energia Atomica, San Martin (Argentina); Consejo Nacional de Investigaciones Cientificas y Tecnicas, Buenos Aires (Argentina); Escuela de Ciencia y Tecnologia, Universidad Nacional de San Martin (Argentina)], E-mail: email@example.com
The Impact of Individual In Vivo Repair of DNA Double-Strand Breaks on Oral Mucositis in Adjuvant Radiotherapy of Head-and-Neck Cancer
Purpose: To evaluate the impact of individual in vivo DNA double-strand break (DSB) repair capacity on the incidence of severe oral mucositis in patients with head-and-neck cancer undergoing adjuvant radiotherapy (RT) or radiochemotherapy (RCT). Patients and Methods: Thirty-one patients with resected head-and-neck cancer undergoing adjuvant RT or RCT were examined. Patients underwent RT of the primary tumor site and locoregional lymph nodes with a total dose of 60-66 Gy (single dose 2 Gy, five fractions per week). Chemotherapy consisted of two cycles of cisplatin and 5-fluorouracil. To assess DSB repair, {gamma}-H2AX foci in blood lymphocytes were quantified before and 0.5 h, 2.5 h, 5 h, and 24 h after in vivo radiation exposure (the first fraction of RT). World Health Organization scores for oral mucositis were documented weekly and correlated with DSB repair. Results: Sixteen patients received RT alone; 15 patients received RCT. In patients who developed Grade {>=} 3 mucositis (n = 18) the amount of unrepaired DSBs 24 h after radiation exposure and DSB repair half-times did not differ significantly from patients with Grade {<=}2 mucositis (n = 13). Patients with a proportion of unrepaired DSBs after 24 h higher than the mean value + one standard deviation had an increased incidence of severe oral mucositis. Conclusions: Evaluation of in vivo DSB repair by determination of {gamma}-H2AX foci loss is feasible in clinical practice and allows identification of patients with impaired DSB repair. The incidence of oral mucositis is not closely correlated with DSB repair under the evaluated conditions.
Fleckenstein, Jochen, E-mail: email@example.com [Department of Radiotherapy and Radiation Oncology, Saarland University Medical School, Homburg (Germany); Kuehne, Martin; Seegmueller, Katharina; Derschang, Sarah; Melchior, Patrick [Department of Radiotherapy and Radiation Oncology, Saarland University Medical School, Homburg (Germany); Graeber, Stefan [Institute for Medical Biometry, Epidemiology und Medical Informatics (IMBEI), Saarland University Medical School, Homburg (Germany); Fricke, Andreas; Ruebe, Claudia E.; Ruebe, Christian [Department of Radiotherapy and Radiation Oncology, Saarland University Medical School, Homburg (Germany)
Differences in Electrostatic Potential Around DNA Fragments Containing Guanine and 8-oxo-Guanine
hanges of electrostatic potential (EP) around the DNA molecule resulting from chemical modifications of nucleotides may play a role in enzymatic recognition of damaged sites. Effects of chemical modifications of nucleotides on the structure of DNA have been characterized through large scale density functional theory computations. Quantum mechanical structural optimizations of DNA fragments with three pairs of nucleotoides and accompanying counteractions were performed with a B3LYP exchange-correlation functional and 6-31G** basis sets. The �intact� DNA fragment contained guanine in the middle layer, while the �damaged� fragment had the guanine replaced with 8-oxo-guanine. The electrostatic potential around these DNA fragments was projected on a surface around the double helix. The 2D maps of EP of intact and damaged DNA fragments were analyzed to identify these modifications of EP that result from the occurrence of 8-oxo-guanine. It was found that distortions of the phosphate groups and displacements of the accompanying countercations are clearly reflected in the EP maps.
Haranczyk, Maciej; Gutowski, Maciej S.
Concentration-Temperature Superposition of Helix Folding Rates in Gelatin
We study the kinetics of helix-coil transition in water solutions of gelatin (collagen protein) by optical rotation techniques combined with thermal characterization. By examining the rates of secondary helix folding, and covering a very wide range of solution concentrations, we are able to identify a universal exponential dependence of folding rate on concentration and quench temperature. We demonstrate a new concentration-temperature superposition of data at all temperatures and concentrations, and build the corresponding master curve. The results support the concept of a diffuse helix-coil transition. We find no concentration dependance of the normalized rate constant, suggesting first order (single) kinetics of secondary helix folding dominate in the early stages of renaturation.
J. L. Gornall; E. M. Terentjev
Kinetically driven helix formation during the homopolymer collapse process
Using Langevin simulations, we find that simple 'generic' bead-and-spring homopolymer chains in a sufficiently bad solvent spontaneously develop helical order during the process of collapsing from an initially stretched conformation. The helix formation is initiated by the unstable modes of the straight chain, which drive the system towards a long-lived metastable transient state. The effect is most pronounced if hydrodynamic interactions are screened.
Sid Ahmed Sabeur; Fatima Hamdache; Friederike Schmid
Solving the Double Digestion Problem as a MixedInteger Linear Program \\Lambda
Solving the Double Digestion Problem as a Mixed�Integer Linear Program \\Lambda Zhijun Wu y and Yin Zhang z August, 2001 Abstract. The double digestion problem for DNA restriction mapping is known�scale double digestion problems. Key Words. DNA sequencing, restriction mapping, double digestion, NP
Zhang, Yin
E-Print Network 3.0 - alpha helix-coil phase Sample Search Results
of Physics, Yeshiva University Collection: Physics 34 Polymer principles and protein folding KEN A. DILL1 Summary: an understanding of the helix-coil transition. For many...
Nature Methods A primer to scaffolded DNA origami
Scaffold / staple layout for a tetrameric 60 helix bundle. Supplementary Protocol 1 Step-by-step scaffold production from phage DNA. Supplementary Protocol 2 Setting up folding reactions. Supplementary Protocol 3. Generated with caDNAno v0.2 Nature Methods: doi.10.1038/nmeth.1570 #12;Detailed scaffold / staple lay
Dietz, Hendrik
Towards 3D modeling of interacting TM helix pairs based on
% of currently known protein structures are from TM proteins [2]. Thus, the lack of experimental structures. Spatial structures of transmembrane proteins are difficult to obtain either experimentally of many transmembrane proteins, is essential in the modeling. Majority of helix-helix interactions
Nebel, Jean-Christophe
Helix Dipole Movement and Conformational Variability Contribute to Allosteric GDP Release in G[alpha] Subunits
Heterotrimeric G proteins (Galphabetagamma) transmit signals from activated G protein-coupled receptors (GPCRs) to downstream effectors through a guanine nucleotide signaling cycle. Numerous studies indicate that the carboxy-terminal alpha5 helix of Galpha subunits participates in Galpha-receptor binding, and previous EPR studies suggest this receptor-mediated interaction induces a rotation and translation of the alpha5 helix of the Galpha subunit [Oldham, W. M., et al. (2006) Nat. Struct. Mol. Biol. 13, 772-777]. On the basis of this result, an engineered disulfide bond was designed to constrain the alpha5 helix of Galpha(i1) into its EPR-measured receptor-associated conformation through the introduction of cysteines at position 56 in the alpha1 helix and position 333 in the alpha5 helix (I56C/Q333C Galpha(i1)). A functional mimetic of the EPR-measured alpha5 helix dipole movement upon receptor association was additionally created by introduction of a positive charge at the amino terminus of this helix, D328R Galpha(i1). Both proteins exhibit a dramatically elevated level of basal nucleotide exchange. The 2.9 A resolution crystal structure of I56C/Q333C Galpha(i1) in complex with GDP-AlF(4)(-) reveals the shift of the alpha5 helix toward the guanine nucleotide binding site that is anticipated by EPR measurements. The structure of the I56C/Q333C Galpha(i1) subunit further revealed altered positions for the switch regions and throughout the Galpha(i1) subunit, accompanied by significantly elevated crystallographic temperature factors. Combined with previous evidence in the literature, the structural analysis supports the critical role of electrostatics of the alpha5 helix dipole and overall conformational variability during nucleotide release.
Preininger, Anita M.; Funk, Michael A.; Oldham, William M.; Meier, Scott M.; Johnston, Christopher A.; Adhikary, Suraj; Kimple, Adam J.; Siderovski, David P.; Hamm, Heidi E.; Iverson, Tina M.; (Vanderbilt); (UNC)
Proofreading RNA: Structure of RNA Polymerase II's Backtracked...
Print Proofreading Ensures Functional Proteins Genes encoded in DNA are made up of nucleotides wound into a double helix of complementary nucleotide, or base, pairs. Converting...
Nanotechnology with DNA DNA Nanodevices
Nanotechnology with DNA DNA Nanodevices Friedrich C. Simmel* and Wendy U. Dittmer A DNA actuator. Introduction.............285 2. Overview: DNA Nanotechnology.......285 3. Prototypes of Nanomechanical DNA overview of DNA nanotechnology as a whole is given. The most important properties of DNA molecules
Ludwig-Maximilians-Universität, München
Recognition of Duplex RNA by Helix-Threading Peptides Barry D. Gooch and Peter A. Beal*
Recognition of Duplex RNA by Helix-Threading Peptides Barry D. Gooch and Peter A. Beal 84112-0850 Received April 15, 2004; E-mail: email@example.com Abstract: Many important biological
Beal, Peter A.
ancient human dna: Topics by E-print Network
STUDY OF DNA DOUBLE-STRAND BREAKS IN BYSTANDER PRIMARY HUMAN FIBROBLASTS L. B. Smilenov-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of...
ancient dna studies: Topics by E-print Network
Knots in the Helix Nebula found in H2
We present a deep and wide field-of-view (4'x 7') image of the planetary nebula (PN) NGC 7293 (the Helix Nebula) in the 2.12 micron H2 v=1-0 S(1) line. The excellent seeing (0.4'') at the Subaru Telescope, allows the details of cometary knots to be examined. The knots are found at distances of 2.2'-6.4' from the central star (CS). At the inner edge and in the inner ring (up to 4.5' fromthe CS), the knot often show a `tadpole' shape, an elliptical head with a bright crescent inside and a long tail opposite to the CS. In detail, there are variations in the tadpole shapes, such as narrowing tails, widening tails, meandering tails, or multi-peaks within a tail. In the outer ring (4.5'-6.4' from the CS), the shapes are more fractured, and the tails do not collimate into a single direction. The transition in knot morphology from the inner edge to the outer ring is clearly seen. The number density of knots governs the H2 surface brightness in the inner ring: H2 exists only within the knots. Possible mechanisms which...
Matsuura, M; McHunu, B M; Tanaka, I; Wright, N J; Smith, M D; Zijlstra, A A; Viti, S; Wesson, R
Mitigating security issues in the evolving DNA synthesis industry
DNA synthesis technologies are advancing at exponential rates, with production of ever longer, more complex, and less expensive sequences of double stranded DNA. This has fostered development of industrial scale design, ...
Turlington, Ralph Donald, III
auger electron-induced double-strand: Topics by E-print Network
Elevated temperature triggers human respiratory syncytial virus F protein six-helix bundle formation
Human respiratory syncytial virus (RSV) is a major cause of severe lower respiratory tract infection in infants, immunocompromised patients, and the elderly. The RSV fusion (F) protein mediates fusion of the viral envelope with the target cell membrane during virus entry and is a primary target for antiviral drug and vaccine development. The F protein contains two heptad repeat regions, HR1 and HR2. Peptides corresponding to these regions form a six-helix bundle structure that is thought to play a critical role in membrane fusion. However, characterization of six-helix bundle formation in native RSV F protein has been hindered by the fact that a trigger for F protein conformational change has yet to be identified. Here we demonstrate that RSV F protein on the surface of infected cells undergoes a conformational change following exposure to elevated temperature, resulting in the formation of the six-helix bundle structure. We first generated and characterized six-helix bundle-specific antibodies raised against recombinant peptides modeling the RSV F protein six-helix bundle structure. We then used these antibodies as probes to monitor RSV F protein six-helix bundle formation in response to a diverse array of potential triggers of conformational changes. We found that exposure of 'membrane-anchored' RSV F protein to elevated temperature (45-55 deg. C) was sufficient to trigger six-helix bundle formation. Antibody binding to the six-helix bundle conformation was detected by both flow cytometry and cell-surface immunoprecipitation of the RSV F protein. None of the other treatments, including interaction with a number of potential receptors, resulted in significant binding by six-helix bundle-specific antibodies. We conclude that native, untriggered RSV F protein exists in a metastable state that can be converted in vitro to the more stable, fusogenic six-helix bundle conformation by an increase in thermal energy. These findings help to better define the mechanism of RSV F-mediated membrane fusion and have important implications for the identification of therapeutic strategies and vaccines targeting RSV F protein conformational changes.
Yunus, Abdul S.; Jackson, Trent P.; Crisafi, Katherine; Burimski, Irina; Kilgore, Nicole R.; Zoumplis, Dorian; Allaway, Graham P.; Wild, Carl T. [Panacos Pharmaceuticals, Inc., 209 Perry Parkway, Suite 7, Gaithersburg, MD 20877 (United States); Salzwedel, Karl, E-mail: email@example.com [Panacos Pharmaceuticals, Inc., 209 Perry Parkway, Suite 7, Gaithersburg, MD 20877 (United States)
Sustainability Double Degree Double Degree Info
Sustainability Double Degree Double Degree Info: · 36 credits in B for graduation. Sustainability Core: Take each course below for a total of 17 -20 credits. Term/Grade Course _____ ____ *NR 350 (4) Sustainable
Grünwald, Niklaus J.
Translocation of molecules into cells by pH-dependent insertion of a transmembrane helix
control, or cell regulation. drug delivery peptide nucleic acid delivery tumors membrane transport helix cellular processes, such as signal and energy transduction, active transport, ion flow, cellcell inter. We used the membrane- impermeable dithionite ion (S2O4 2), which can chemically modify the 4-chloro-7
Helix Stabilization Precedes Aqueous and Bilayer-Catalyzed Fiber Formation in Islet
; received in revised form 12 July 2009; accepted 27 July 2009 Available online 30 July 2009 Islet amyloid-terminal disulfide bond (Cys2�Cys7) decreases the extent of helix formed throughout this region. Through manipulation microglobulin in the joints of end-stage renal disease patients, and islet amyloid polypeptide (IAPP
Miranker, Andrew
An inverse correlation between loop length and stability in a four-helix-bundle protein
their intrinsic func- tionality upon their new environmental context: the calcium-binding activity of a loop has the molten globule behavior of apo- - lactalbumin in the absence of calcium [18]. Conversely, loops have beenAn inverse correlation between loop length and stability in a four-helix-bundle protein Athena D
Mochrie, Simon
Helix-Sense Selective Polymerization of Carbodiimides: Building Permanently Optically Active Polymers from Achiral Monomers
Helix-Sense Selective Polymerization of Carbodiimides: Building Permanently Optically Active polymerizations of achiral monomers using chiral catalysts.8 For this approach to be viable, the helical the polymerization of achiral carbodiimides with optically active catalysts to yield stable helical materials
Patterning nanocrystals using DNA
One of the goals of nanotechnology is to enable programmed self-assembly of patterns made of various materials with nanometer-sized control. This dissertation describes the results of experiments templating arrangements of gold and semiconductor nanocrystals using 2'-deoxyribonucleic acid (DNA). Previously, simple DNA-templated linear arrangements of two and three nanocrystals structures have been made.[1] Here, we have sought to assemble larger and more complex nanostructures. Gold-DNA conjugates with 50 to 100 bases self-assembled into planned arrangements using strands of DNA containing complementary base sequences. We used two methods to increase the complexity of the arrangements: using branched synthetic doublers within the DNA covalent backbone to create discrete nanocrystal groupings, and incorporating the nanocrystals into a previously developed DNA lattice structure [2][3] that self-assembles from tiles made of DNA double-crossover molecules to create ordered nanoparticle arrays. In the first project, the introduction of a covalently-branched synthetic doubler reagent into the backbone of DNA strands created a branched DNA ''trimer.'' This DNA trimer templated various structures that contained groupings of three and four gold nanoparticles, giving promising, but inconclusive transmission electron microscopy (TEM) results. Due to the presence of a variety of possible structures in the reaction mixtures, and due to the difficulty of isolating the desired structures, the TEM and gel electrophoresis results for larger structures having four particles, and for structures containing both 5 and 10 nm gold nanoparticles were inconclusive. Better results may come from using optical detection methods, or from improved sample preparation. In the second project, we worked toward making two-dimensional ordered arrays of nanocrystals. We replicated and improved upon previous results for making DNA lattices, increasing the size of the lattices to a length greater than 20 {micro}m, and collecting atomic force microscopy (AFM) images up to 30 {micro}m. We found the lattices' requirement of divalent magnesium cations to stabilize Holliday junctions to be incompatible with the stability of charge-stabilized gold nanoparticles used for the experiments here, and gold particles added indiscriminately to the lattice surface through non-specific binding. Redesigning the lattices to avoid magnesium may improve results.
Williams, Shara Carol
The Crystal Structure of TAL Effector PthXo1 Bound to Its DNA Target
DNA recognition by TAL effectors is mediated by tandem repeats, each 33 to 35 residues in length, that specify nucleotides via unique repeat-variable diresidues (RVDs). The crystal structure of PthXo1 bound to its DNA target was determined by high-throughput computational structure prediction and validated by heavy-atom derivatization. Each repeat forms a left-handed, two-helix bundle that presents an RVD-containing loop to the DNA. The repeats self-associate to form a right-handed superhelix wrapped around the DNA major groove. The first RVD residue forms a stabilizing contact with the protein backbone, while the second makes a base-specific contact to the DNA sense strand. Two degenerate amino-terminal repeats also interact with the DNA. Containing several RVDs and noncanonical associations, the structure illustrates the basis of TAL effector-DNA recognition.
Mak, Amanda Nga-Sze; Bradley, Philip; Cernadas, Raul A.; Bogdanove, Adam J.; Stoddard, Barry L. (FHCRC); (Iowa State)
DNA demethylation by DNA repair
Active DNA demethylation underlies key facets of reproduction in flowering plants and mammals and serves a general genome housekeeping function in plants. A family of 5-methylcytosine DNA glycosylases catalyzes plant ...
Gehring, Mary
The Triple Helix Model and the Meta-Stabilization of Urban Technologies in Smart Cities
The Triple Helix model of university-industry-government relations can be generalized from a neo-institutional model of networks to a neo-evolutionary model of how three selection environments operate upon one another. The neo-evolutionary model enables us to appreciate both organizational integration in university-industry-government relations and differentiation among functions like the generation of intellectual capital, creation of wealth, and their attending legislation. The specification of innovation systems in terms of nations, sectors, cities, and regions can then be formulated as empirical questions: is synergy generated among functions in networks of relations? This Triple Helix model enables us to study the knowledge base of an urban economy in terms of a trade-off between locally stabilized and (potentially locked-in) trajectories versus the techno-economic and cultural development regimes which work with one more degree of freedom at the global level. The meta-stabilizing potentials of urban tec...
Leydesdorff, Loet
Testing the helix model for protein folding on four simple proteins
We test a simplified, local version of the helix model on two synthetic and two natural proteins, to study its efficiency in predicting the native secondary structure. The results we obtain are very good for the synthetic sequences, poorer for the two natural ones. This suggests that non-local terms play a fundamental role in determining the secondary structure, even if in some cases local terms alone may be sufficient.
Pierpaolo Bruscolini
The earliest events in protein folding: Helix dynamics in proteins and model peptides
The earliest events in protein folding are critically important in determining the folding pathway, but have proved difficult to study by conventional approaches. We have developed new rapid initiation methods and structure-specific probes to interrogate the earliest events of protein folding. Our focus is the pathways. Folding or unfolding reactions are initiated on a fast timescale (10 ns) using a laser induced temperature jump (15 C) and probed with time-resolved infrared spectroscopy. We obtained the kinetics of the helix-coil transition for a model 21-residue peptide. The observed rate constant k{sub obs} = k{sub f} + k{sub u} for reversible kinetics; from the observed rate (6 x 10{sup 6} s{sup -1}) and the equilibrium constant favoring folding of 7.5 at 27 C, we calculate a folding lifetime of 180 ns and an unfolding lifetime of 1.4 {mu}s. The {open_quotes}molten globule{close_quotes} form of apomyoglobin (horse, pH*3, 0.15M NaCl) shows similar kinetics for helix that is unconstrained by tertiary structure (helix with an unusually low Amide I frequency, near 1633 cm{sup -1}). In {open_quotes}native{close_quotes} apomyoglobin (horse, pH*5.3, 10 mM NaCl) two very different rates (45 ns and 70 {mu}s) are observed and we infer that a third occurs on a timescales inaccessible to our experiment (> 1 ms). We suggest that the slower processes are due to helix formation that is rate-limited by the formation of tertiary structure.
Dyer, R.B.; Williams, S.; Woodruff, W.H. [Los Alamos National Lab., NM (United States)] [and others
Double Beta Decay: Scintillators
Scintillator detectors can be used in experiments searching for neutrinoless double beta decay. A wide variety of double beta decay candidate isotopes can be made into scintillators or can be loaded into scintillators. Experimental programs developing liquid xenon, inorganic crystals, and Nd-loaded liquid scintillator are described in this review. Experiments with 48Ca and 150Nd benefit from their high endpoint which places the neutrinoless double beta decay signal above most backgrounds from natural radioactivity.
Mark C. Chen
Mass spectrometric characterization of sequence-specific complexes of DNA and transcription factor PU.1 DNA binding domain
Electrospray ionization mass spectrometry (ESI-MS) has been used to study the noncovalent interaction of the 13.5-kDa DNA binding domain of PU.1 (PU.1-DBD) with specific double-stranded DNA (dsDNA) target molecules. Mixtures of PU.1-DBD protein and wildtype target DNA sequence yielded ESI-MS spectra showing only protein-dsDNA complex ions of 1:1 stoichiometry and free dsDNA. When PU.1-DBD protein, wild type target DNA, and a mutant target DNA lacking the consensus sequence were mixed, only the 1:1 complex with the wild-type DNA was observed, consistent with gel electrophoresis mobility shift assay results, demonstrating the observation of sequence-specific protein-dsDNA complexes using ESI-MS. 22 refs., 5 figs., 1 tab.
Cheng, Xueheng; Harms, A.C.; Bruce, J.E. [Pacific Northwest National Lab., Richland, WA (United States)] [and others] [Pacific Northwest National Lab., Richland, WA (United States); and others
Double beta decay experiments
The present status of double beta decay experiments are reviewed. The results of the most sensitive experiments, NEMO-3 and CUORICINO, are discussed. Proposals for future double beta decay experiments are considered. In these experiments sensitivity for the effective neutrino mass will be on the level of (0.1-0.01) eV.
A. S. Barabash
The present status of double beta decay experiments is reviewed. The results of the most sensitive experiments are discussed. Proposals for future double beta decay experiments with a sensitivity to the $$ at the level of (0.01--0.1) eV are considered.
Oxidation of Helix-3 Methionines Precedes the Formation of PK Resistant PrPSc
cells expressing a chimera Mo/Ha PrP form. (B) Human and mouse normal brain homogenates were immunoblotted with the RGM antibody alone or preincubated with several PrP peptides in the Helix-3 Met area. (C) Immunoblots of HuPrP(23�230) wt and E200K with 3...P conversion may link the activity of the prion proteins with other neurodegenerative conditions affected by stress and oxidation, such as ALS, AD and Parkinson�s diseases [2,52,53], as well as to normal aging [45]. Materials and Methods Ethical statement...
Canello, Tamar; Frid, Kati; Gabizon, Ronen; Lisa, Silvia; Friedler, Assaf; Moskovitz, Jackob; Gasset, Marí a; Gabizon, Ruth
Neutron scattering study of the hydration hull of DNA by H/sub 2/O/D/sub 2/O-exchange
Films of highly oriented DNA (A-, B-, and C-conformation) were investigated by neutron scattering. Diffuse sheets corresponding to a one-dimensionally periodic ''water-DNA'' structure are identified close to the intersection of the helix direction with the structure factor maximum of bulk water. The correlation length in this structure is strongly influenced by its commensurability with the axial translation H per nucleotide. The fiber or lateral chain interaction is enhanced in the commensurate case. 10 refs., 3 figs.
Grimm, H.; Stiller, H.; Majkrzak, C.F.; Rupprecht, A.
Neutrinoless double beta decay
The physics potential of neutrinoless double beta decay is discussed. Furthermore, experimental considerations are presented as well as the current status of experiments. Finally an outlook towards the future, work on nuclear matrix elements and alternative processes is given.
K. Zuber
Neutrinoless Double $?$-Decay
The neutrinoless double $\\beta$-decay is reviewed. Model independent evidence in favor of neutrino masses and mixing is briefly summarized. The data of the recent experiments on the search for $0\
S. M. Bilenky
Double Beta Decay
The motivation, present status, and future plans of the search for the neutrinoless double beta decay are reviewed. It is argued that, motivated by the recent observations of neutrino oscillations, there is a reasonable hope that neutrinoless double beta decay corresponding to the neutrino mass scale suggested by oscillations, of about 50 meV, actually exists. The challenges to achieve the sensitivity corresponding to this mass scale, and plans to overcome them, are described.
Steven R. Elliott; Petr Vogel
Interpretation of the Helix Planetary Nebula using Hydro-Gravitational-Dynamics: Planets and Dark Energy
Hubble Space Telescope images of the Helix Planetary Nebula are interpreted using the hydro-gravitational-dynamics theory (HGD) of Gibson 1996-2006. HGD claims that baryonic-dark-matter (BDM) dominates the halo masses of galaxies (Schild 1996) as Jovian (Primordial-fog-particle [PFP]) Planets (JPPs) in proto-globular-star-cluster (PGC) clumps for all galaxy halo diameters bounded by stars. From HGD, supernova Ia (SNe Ia) events always occur in planetary nebulae (PNe) within PGCs. The dying central star of a PNe slowly accretes JPP mass to grow the white-dwarf to instability. Plasma jets, winds and radiation driven by contraction and spin-up of the carbon star evaporate JPPs revealing its Oort accretional cavity. SNe Ia events may thus be obscured or not obscured by radiation-inflated JPP atmospheres producing systematic SNe Ia distance errors, so the otherwise mysterious ``dark energy'' concept is unnecessary. HST/ACS and WFPC2 Helix images show >7,000 cometary globules and SST/IRAC images show >20,000-40,000, here interpreted as gas-dust cocoons of JPPs evaporated by the spin powered radiation of the PNe central white-dwarf. Observed JPP masses fossilizes the primordial density existing when the plasma universe fragmented into proto-superclusters, proto-clusters, and proto-galaxies. Pulsar scintillation spectra support the postulated multi-planet atmospheres.
Carl H. Gibson; Rudolph E. Schild
Nucleotide-dependent displacement and dynamics of the ?-1 helix in kinesin revealed by site-directed spin labeling EPR
Highlights: �Dipolar EPR detects the distance between the spin-labeled kinesin ?-1 and ?-2 helices. �The distance has at least two populations: 1.5 nm (in crystal form: 20%) and >2.5 nm. �The short distance conformer was populated 40% in the apo state with microtubules. �ATP analog or ADP binding caused the 1.5 nm distance to be less populated (?20%). �The ?-1 helix moves closer to the neck-linker (away from ?-2) to facilitate docking. -- Abstract: In kinesin X-ray crystal structures, the N-terminal region of the ?-1 helix is adjacent to the adenine ring of the bound nucleotide, while the C-terminal region of the helix is near the neck-linker (NL). Here, we monitor the displacement of the ?-1 helix within a kinesin monomer bound to microtubules (MTs) in the presence or absence of nucleotides using site-directed spin labeling EPR. Kinesin was doubly spin-labeled at the ?-1 and ?-2 helices, and the resulting EPR spectrum showed dipolar broadening. The inter-helix distance distribution showed that 20% of the spins have a peak characteristic of 1.4�1.7 nm separation, which is similar to what is predicted from the X-ray crystal structure, albeit 80% were beyond the sensitivity limit (>2.5 nm) of the method. Upon MT binding, the fraction of kinesin exhibiting an inter-helix distance of 1.4�1.7 nm in the presence of AMPPNP (a non-hydrolysable ATP analog) and ADP was 20% and 25%, respectively. In the absence of nucleotide, this fraction increased to 40�50%. These nucleotide-induced changes in the fraction of kinesin undergoing displacement of the ?-1 helix were found to be related to the fraction in which the NL undocked from the motor core. It is therefore suggested that a shift in the ?-1 helix conformational equilibrium occurs upon nucleotide binding and release, and this shift controls NL docking onto the motor core.
Yasuda, Satoshi; Yanagi, Takanori [Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043 (Japan)] [Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043 (Japan); Yamada, Masafumi D. [Division of Bioengineering, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577 (Japan)] [Division of Bioengineering, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577 (Japan); Ueki, Shoji [Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, Shido 1314-1, Samuki, Kagawa 769-2193 (Japan)] [Kagawa School of Pharmaceutical Sciences, Tokushima Bunri University, Shido 1314-1, Samuki, Kagawa 769-2193 (Japan); Maruta, Shinsaku [Division of Bioengineering, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577 (Japan)] [Division of Bioengineering, Graduate School of Engineering, Soka University, Hachioji, Tokyo 192-8577 (Japan); Inoue, Akio [Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043 (Japan)] [Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043 (Japan); Arata, Toshiaki, E-mail: firstname.lastname@example.com [Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043 (Japan)] [Department of Biological Sciences, Graduate School of Science, Osaka University, Toyonaka, Osaka 560-0043 (Japan)
Observation of acoustic umklapp-phonons in water-stabilized DNA by neutron scattering
We have investigated static and dynamic properties of wet-spun DNA fibers in their C, A, and B conformations using the thermal neutron scattering. Over-damped and underdamped longitudinal acoustic umklapp phonons are observed in the vicinity of a hydration-dependent two-dimensional sheet of diffuse scattering perpendicular to the helix axis. A quantitative analysis of this mode is presented for the B-DNA sample in terms of a harmonic one-dimensional liquid, i.e., taking into account all orders of fluctuations. The resulting effective dispersion does not extrapolate to zero frequency at the position of the sheet.
Grimm, H.; Stiller, H.; Majkrzak, C.F.; Rupprecht, A.; Dahlborg, U.
The role of histones and histone modifying enzymes in ribosomal dna silencing in saccharomyces cerevisiae
DNA silencing. We found that, unlike Sir2 that represses both Pol II transcription and recombination, histone H1 only represses recombination at the rDNA. The hyperrecombination defect at the rDNA is more severe in sir2? hho1? double mutant than in either single...
Li, Chonghua
DOUBLE MAJORS Imaging Science + ...
DOUBLE MAJORS Imaging Science + ... Applied Mathematics Biomedical Sciences Computer Science Undergraduate Research Internships and Cooperative Education (Co-op) (optional) Study Abroad WHY IMAGING SCIENCE Science: BS, MS, PhD Color Science: MS, PhD BS + MS/PhD Combos HUMAN VISION BIO- MEDICAL ASTRO- PHYSICS
Zanibbi, Richard
The status of the search for neutrinoless double beta decay is reviewed. The effort to reach the sensitivity needed to cover the effective Majorana neutrino mass corresponding to the degenerate and inverted mass hierarchy is described. Various issues concerning the theory (and phenomenology) of the relation between the $0\
Petr Vogel
the search for neutrinoless double beta decay may prove verySearching for neutrinoless double beta decay is the onlysensitivity of neutrinoless double beta decay. The potential
Kayser, B.
Collection, focusing, and metering of DNA in microchannels using addressable electrode arrays for portable low-power bioanalysis
the funding for this work. vii NOMENCLATURE DNA Deoxyribonucleic Acid dsDNA Double Stranded DNA ssDNA Single Stranded DNA EDTA Ethylene Diamine Tetracetic Acid TBE Tris-Borate EDTA BME Beta Mercapto Ethanol PCB Printed Circuit Board DI Deionized... through a USB cable using a Personal Computer........................................................................................ 66 Figure 27 Top level block diagram of the FPGA based portable analysis device showing flow of information...
Shaikh, Faisal
Enzyme Structure Provides Insights into Cancer and Aging
Enzyme Structure Provides Insights into Cancer and Aging Print XPD helicase is an enzyme that unwinds the DNA double helix; it is one component of an essential repair mechanism...
for doubling solar panel
An outline for doubling solar panel efficiency C o l o ra do S c ho o l of M i ne s Ma g a z i ne Take a look at a solar panel on a sunny Colorado day and, if you're like most people, you won't see physics professor and solar energy researcher, who admits to checking out his panels and their energy
Double Beta Decay Experiments
At present, neutrinoless double beta decay is perhaps the only experiment that can tell us whether the neutrino is a Dirac or a Majorana particle. Given the significance of the 0{nu}{beta}{beta}, there is a widespread interest for these rare event studies employing a variety of novel techniques. This paper describes the current status of DBD experiments. The Indian effort for an underground NDBD experiment at the upcoming INO laboratory is also presented.
Nanal, Vandana [Dept. of Nuclear and Atomic Physics, Tata Institute of Fundamental Research, Mumbai 400 005 (India)
The Era GTPase recognizes the GAUCACCUCC sequence and binds helix 45 near the 3; end of 16S rRNA
Era, composed of a GTPase domain and a K homology domain, is essential for bacterial cell viability. It is required for the maturation of 16S rRNA and assembly of the 30S ribosomal subunit. We showed previously that the protein recognizes nine nucleotides (1531{sup AUCACCUCC}1539) near the 3{prime} end of 16S rRNA, and that this recognition stimulates GTP-hydrolyzing activity of Era. In all three kingdoms of life, the 1530{sup GAUCA}1534 sequence and helix 45 (h45) (nucleotides 1506-1529) are highly conserved. It has been shown that the 1530{sup GA}1531 to 1530{sup AG}1531 double mutation severely affects the viability of bacteria. However, whether Era interacts with G1530 and/or h45 and whether such interactions (if any) contribute to the stimulation of Era's GTPase activity were not known. Here, we report two RNA structures that contain nucleotides 1506-1542 (RNA301), one in complex with Era and GDPNP (GNP), a nonhydrolysable GTP-analogue, and the other in complex with Era, GNP, and the KsgA methyltransferase. The structures show that Era recognizes 10 nucleotides, including G1530, and that Era also binds h45. Moreover, GTPase assay experiments show that G1530 does not stimulate Era's GTPase activity. Rather, A1531 and A1534 are most important for stimulation and h45 further contributes to the stimulation. Although G1530 does not contribute to the intrinsic GTPase activity of Era, its interaction with Era is important for binding and is essential for the protein to function, leading to the discovery of a new cold-sensitive phenotype of Era.
Tu, Chao; Zhou, Xiaomei; Tarasov, Sergey G.; Tropea, Joseph E.; Austin, Brian P.; Waugh, David S.; Court, Donald L.; Ji, Xinhua (NCI)
Structural and mechanistic insights into Mcm2-7 double-hexamer assembly and function
DOE Public Access Gateway for Energy & Science Beta (PAGES Beta)
Eukaryotic cells license each DNA replication origin during G1 phase by assembling a prereplication complex that contains a Mcm2�7 (minichromosome maintenance proteins 2�7) double hexamer. During S phase, each Mcm2�7 hexamer forms the core of a replicative DNA helicase. However, the mechanisms of origin licensing and helicase activation are poorly understood. The helicase loaders ORC�Cdc6 function to recruit a single Cdt1�Mcm2�7 heptamer to replication origins prior to Cdt1 release and ORC�Cdc6�Mcm2�7 complex formation, but how the second Mcm2�7 hexamer is recruited to promote double-hexamer formation is not well understood. Here, structural evidence for intermediates consisting of an ORC�Cdc6�Mcm2�7 complex and an ORC�Cdc6�Mcm2�7�Mcm2�7 complex are reported, which together provide new insights into DNA licensing. Detailed structural analysis of the loaded Mcm2�7 double-hexamer complex demonstrates that the two hexamers are interlocked and misaligned along the DNA axis and lack ATP hydrolysis activity that is essential for DNA helicase activity. Moreover, we show that the head-to-head juxtaposition of the Mcm2�7 double hexamer generates a new protein interaction surface that creates a multisubunit-binding site for an S-phase protein kinase that is known to activate DNA replication. The data suggest how the double hexamer is assembled and how helicase activity is regulated during DNA licensing, with implications for cell cycle control of DNA replication and genome stability.
Sun, Jingchuan [Brookhaven National Laboratory (BNL), Upton, NY (United States); Li, Huilin [Brookhaven National Laboratory (BNL), Upton, NY (United States); Stony Brook Univ., Stony Brook, NY (United States); Fernandez-Cid, Alejandra [Imperial College Faculty of Medicine, London (United Kingdom). DNA Replication Group; Riera, Alberto [Imperial College Faculty of Medicine, London (United Kingdom). MRC Clinical Sciences Centre; Tognetti, Sivia [Imperial College Faculty of Medicine, London (United Kingdom). MRC Clinical Sciences Centre; Yuan, Zuanning [Stony Brook Univ., Stony Brook, NY (United States); Stillman, Bruce [Cold Spring Harbor Lab., NY (United States); Speck, Christian [Imperial College Faculty of Medicine, London (United Kingdom). DNA Replication Group
The development of a model of Alpha helix formation for transmembrane peptides
sequence was ordered from integrated DNA Technologtes, Inc. Transformations took place using electroporation and heat shock methods into a competent E. coif cell line. Transformed cells were plated on Luria broth (LB) agar plates containing ampicillin...
Funk, Geoffrey Alexander
576 IEEE TRANSACTIONS ON PLASMA SCIENCE, VOL. 28, NO. 3, JUNE 2000 A Comparison of L-Band Helix TWT Experiments
576 IEEE TRANSACTIONS ON PLASMA SCIENCE, VOL. 28, NO. 3, JUNE 2000 A Comparison of L-Band Helix TWT. Publisher Item Identifier S 0093-3813(00)05375-3. With the projected growth of the telecommunications market
Hassam, Adil
Interpretation of the Helix Planetary Nebula using Hydro-Gravitational Theory
Wide angle Hubble Space Telescope (HST/ACS) images of the Helix Planetary Nebula (NGC 7293) are interpreted using the hydro-gravitational theory (HGT) of Gibson 1996-2000 that predicts the baryonic dark matter and interstellar medium (ISM) consists of Mars-mass primordial-fog-particle (PFP) frozen H-He planets. The new ACS images confirm and extend the O'Dell and Handron 1996 WFPC2 images showing thousands of cometary globules, which we suggest are cocoons of PFP and Jupiter frozen-gas-planets evaporated by powerful beamed radiation from the hot central white dwarf and its companion. The atmosphere mass of the largest cometary globules is ~ 3x10^{25} kg with spacing ~ 10^{14} m, supporting the prediction of HGT that the mass density of the ISM in Galaxy star forming regions should match the large baryonic primordial value at the time of first structure formation (10^{12} s or 30,000 years), with \\rho ~ (3-1)x 10^{-17} kg m^{-3}.
AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr May JunDatastreamsmmcrcalgovInstrumentsruc DocumentationP-Series to UserProduct:Directives Templates8. U.S.Donald R. Baer d37154DoorsDouble
Helix Unwinding and Base Flipping Enable Human MTERF1 to Terminate Mitochondrial Transcription
Defects in mitochondrial gene expression are associated with aging and disease. Mterf proteins have been implicated in modulating transcription, replication and protein synthesis. We have solved the structure of a member of this family, the human mitochondrial transcriptional terminator MTERF1, bound to dsDNA containing the termination sequence. The structure indicates that upon sequence recognition MTERF1 unwinds the DNA molecule, promoting eversion of three nucleotides. Base flipping is critical for stable binding and transcriptional termination. Additional structural and biochemical results provide insight into the DNA binding mechanism and explain how MTERF1 recognizes its target sequence. Finally, we have demonstrated that the mitochondrial pathogenic G3249A and G3244A mutations interfere with key interactions for sequence recognition, eliminating termination. Our results provide insight into the role of mterf proteins and suggest a link between mitochondrial disease and the regulation of mitochondrial transcription.
Yakubovskaya, E.; Mejia, E; Byrnes, J; Hambardjieva, E; Garcia-Diaz, M
Asiakastyytyv�isyystutkimus - DNA Kauppa Lappeenranta.
??Opinn�ytety�n aiheena oli DNA Kauppa Lappeenrannan asiakastyytyv�isyyden tutkiminen. Tutkimuksen ensisijaisena tavoitteena oli selvitt��, kuinka tyytyv�isi� asiakkaat olivat olleet DNA Kauppa Lappeenrannan ja asiakkaan v�liseen kontaktipintaan� (more)
Pitk�nen, Jesse
Neutrinoless Double Phys 135c Spring 2007
Neutrinoless Double Beta Decay Phys 135c Spring 2007 Michael Mendenhall #12;Theory Overview #12 beta decays #12;neutrinoless double beta decays n e- p beta decay e #12;neutrinoless double beta decays n e- p beta decay e n e- p n e- p double beta decay e e #12;neutrinoless double beta decays n e- p
Golwala, Sunil
Binary electrokinetic separation of target DNA from background DNA primers.
This report contains the summary of LDRD project 91312, titled ''Binary Electrokinetic Separation of Target DNA from Background DNA Primers''. This work is the first product of a collaboration with Columbia University and the Northeast BioDefense Center of Excellence. In conjunction with Ian Lipkin's lab, we are developing a technique to reduce false positive events, due to the detection of unhybridized reporter molecules, in a sensitive and multiplexed detection scheme for nucleic acids developed by the Lipkin lab. This is the most significant problem in the operation of their capability. As they are developing the tools for rapidly detecting the entire panel of hemorrhagic fevers this technology will immediately serve an important national need. The goal of this work was to attempt to separate nucleic acid from a preprocessed sample. We demonstrated the preconcentration of kilobase-pair length double-stranded DNA targets, and observed little preconcentration of 60 base-pair length single-stranded DNA probes. These objectives were accomplished in microdevice formats that are compatible with larger detection systems for sample pre-processing. Combined with Columbia's expertise, this technology would enable a unique, fast, and potentially compact method for detecting/identifying genetically-modified organisms and multiplexed rapid nucleic acid identification. Another competing approach is the DARPA funded IRIS Pharmaceutical TIGER platform which requires many hours for operation, and an 800k$ piece of equipment that fills a room. The Columbia/SNL system could provide a result in 30 minutes, at the cost of a few thousand dollars for the platform, and would be the size of a shoebox or smaller.
James, Conrad D.; Derzon, Mark Steven
ATP Hydrolysis and DNA Binding by the Escherichia coli RecF Protein*
ATP Hydrolysis and DNA Binding by the Escherichia coli RecF Protein* (Received for publication ATP hydrolytic activity. ATP hydrolysis leads to RecF dissociation from double-stranded (ds to DNA. A mutant RecF protein that can bind but cannot hydrolyze ATP (RecF K36R) does not readily
Double acting bit holder
A double acting bit holder that permits bits held in it to be resharpened during cutting action to increase energy efficiency by reducing the amount of small chips produced. The holder consist of: a stationary base portion capable of being fixed to a cutter head of an excavation machine and having an integral extension therefrom with a bore hole therethrough to accommodate a pin shaft; a movable portion coextensive with the base having a pin shaft integrally extending therefrom that is insertable in the bore hole of the base member to permit the moveable portion to rotate about the axis of the pin shaft; a recess in the movable portion of the holder to accommodate a shank of a bit; and a biased spring disposed in adjoining openings in the base and moveable portions of the holder to permit the moveable portion to pivot around the pin shaft during cutting action of a bit fixed in a turret to allow front, mid and back positions of the bit during cutting to lessen creation of small chip amounts and resharpen the bit during excavation use.
Morrell, Roger J. (Blommington, MN); Larson, David A. (Minneapolis, MN); Ruzzi, Peter L. (Eagan, MN)
Double Chooz: Latest results
The latest results from the Double Chooz experiment on the neutrino mixing angle $\\theta_{13}$ are presented. A detector located at an average distance of 1050 m from the two reactor cores of the Chooz nuclear power plant has accumulated a live time of 467.90 days, corresponding to an exposure of 66.5 GW-ton-year (reactor power $\\times$ detector mass $\\times$ live time). A revised analysis has boosted the signal efficiency and reduced the backgrounds and systematic uncertainties compared to previous publications, paving the way for the two detector phase. The measured $\\sin^2 2\\theta_{13} = 0.090^{+0.032}_{-0.029}$ is extracted from a fit to the energy spectrum. A deviation from the prediction above a visible energy of 4 MeV is found, being consistent with an unaccounted reactor flux effect, which does not affect the $\\theta_{13}$ result. A consistent value of $\\theta_{13}$ is measured in a rate-only fit to the number of observed candidates as a function of the reactor power, confirming the robustness of the result.
J. I. Crespo-Anad�n; for the Double Chooz collaboration
Derivatized versions of ligase enzymes for constructing DNA sequences
A method of making very long, double-stranded synthetic poly-nucleotides. A multiplicity of short oligonucleotides is provided. The short oligonucleotides are sequentially hybridized to each other. Enzymatic ligation of the oligonucleotides provides a contiguous piece of PCR-ready DNA of predetermined sequence.
Mariella, Jr., Raymond P. (Danville, CA); Christian, Allen T. (Tracy, CA); Tucker, James D. (Novi, MN); Dzenitis, John M. (Livermore, CA); Papavasiliou, Alexandros P. (Oakland, CA)
APOBEC3G: a Double Agent in Defense Harold C. Smith
APOBEC3G: a Double Agent in Defense Harold C. Smith Department of Biochemistry and Biophysics to A3G having a requirement for a ssDNA Corresponding Author contacts: Harold C. Smith, Department and Dentistry, Rochester, NY 14642 USA, firstname.lastname@example.com Voice: (585) 275-4267, FAX: (585) 275
Levin, Judith G.
Does quantum entanglement in DNA synchronize the catalytic centers of type II restriction endonucleases?
Several living systems have been examined for their apparent optimization of structure and function for quantum behavior at biological length scales. Orthodox type II endonucleases, the largest class of restriction enzymes, recognize four-to-eight base pair sequences of palindromic DNA, cut both strands symmetrically, and act without an external metabolite such as ATP. While it is known that these enzymes induce strand breaks by attacking phosphodiester bonds, what remains unclear is the mechanism by which cutting occurs in concert at the catalytic centers. Previous studies indicate the primacy of intimate DNA contacts made by the specifically bound enzyme in coordinating the two synchronized cuts. We propose that collective electronic behavior in the DNA helix generates coherent oscillations, quantized through boundary conditions imposed by the endonuclease, that provide the energy required to break two phosphodiester bonds. Such quanta may be preserved in the presence of thermal noise and electromagnetic interference through decoherence shielding, the specific complex's exclusion of water and ions surrounding the helix. Clamping energy imparted by the enzyme decoherence shield is comparable with zero-point modes of the dipole-dipole oscillations in the DNA recognition sequence. The palindromic mirror symmetry of this sequence should conserve parity during the process. Experimental data corroborate that symmetric bond-breaking ceases when the symmetry of the endonuclease complex is violated, or when environmental parameters are perturbed far from biological optima. Persistent correlation between states in DNA sequence across spatial separations of any length--a characteristic signature of quantum entanglement--may be explained by such a physical mechanism.
P. Kurian; G. Dunston; J. Lindesay
Efficient and simpler method to construct normalized cDNA libraries with improved representations of full-length cDNAs
This invention provides a method to normalize a cDNA library comprising: (a) constructing a directionally cloned library containing cDNA inserts wherein the insert is capable of being amplified by polymerase chain reaction; (b) converting a double-stranded cDNA library into single-stranded DNA circles; (c) generating single-stranded nucleic acid molecules complementary to the single-stranded DNA circles converted in step (b) by polymerase chain reaction with appropriate primers; (d) hybridizing the single-stranded DNA circles converted in step (b) with the complementary single-stranded nucleic acid molecules generated in step (c) to produce partial duplexes to an appropriate Cot; and (e) separating the unhybridized single-stranded DNA circles from the hybridized DNA circles, thereby generating a normalized cDNA library. This invention also provides a method to normalize a cDNA library wherein the generating of single-stranded nucleic acid molecules complementary to the single-stranded DNA circles converted in step (b) is by excising cDNA inserts from the double-stranded cDNA library; purifying the cDNA inserts from cloning vectors; and digesting the cDNA inserts with an exonuclease. This invention further provides a method to construct a subtractive cDNA library following the steps described above. This invention further provides normalized and/or subtractive cDNA libraries generated by the above methods. 25 figs.
Soares, M.B.; Fatima Bonaldo, M. de
This invention provides a method to normalize a cDNA library comprising: (a) constructing a directionally cloned library containing cDNA inserts wherein the insert is capable of being amplified by polymerase chain reaction; (b) converting a double-stranded cDNA library into single-stranded DNA circles; (c) generating single-stranded nucleic acid molecules complementary to the single-stranded DNA circles converted in step (b) by polymerase chain reaction with appropriate primers; (d) hybridizing the single-stranded DNA circles converted in step (b) with the complementary single-stranded nucleic acid molecules generated in step (c) to produce partial duplexes to an appropriate Cot; and (e) separating the unhybridized single-stranded DNA circles from the hybridized DNA circles, thereby generating a normalized cDNA library. This invention also provides a method to normalize a cDNA library wherein the generating of single-stranded nucleic acid molecules complementary to the single-stranded DNA circles converted in step (b) is by excising cDNA inserts from the double-stranded cDNA library; purifying the cDNA inserts from cloning vectors; and digesting the cDNA inserts with an exonuclease. This invention further provides a method to construct a subtractive cDNA library following the steps described above. This invention further provides normalized and/or subtractive cDNA libraries generated by the above methods.
Soares, Marcelo Bento (New York, NY); Bonaldo, Maria de Fatima (New York, NY)
Neutrinoless Double Beta-Decay
The neutrinoless double $\\beta$-decay of nuclei is reviewed. We discuss neutrino mixing and 3x3 PMNS neutrino mixing matrix. Basic theory of neutrinoless double $\\beta$-decay is presented in some details. Results of different calculations of nuclear matrix element are discussed. Experimental situation is considered. The Appendix is dedicated to E. Majorana (brief biography and his paper in which the theory of Majorana particles is given)
Structural insight into recruitment of translesion DNA polymerase Dpo4 to sliding clamp PCNA
DNA polymerases are co-ordinated by sliding clamps (PCNA/{beta}-clamp) in translesion synthesis. It is unclear how these enzymes assemble on PCNA with geometric and functional compatibility. We report the crystal structure of a full-length Y-family polymerase, Dpo4, in complex with heterodimeric PCNA1-PCNA2 at 2.05 {angstrom} resolution. Dpo4 exhibits an extended conformation that differs from the Dpo4 structures in apo- or DNA-bound form. Two hinges have been identified in Dpo4, which render the multidomain polymerase flexible conformations and orientations relative to PCNA. Dpo4 binds specifically to PCNA1 on the conserved ligand binding site. The C-terminal peptide of Dpo4 becomes structured with a 3{sub 10} helix and dominates the specific binding. The Y-family polymerase also contacts PCNA1 with its finger, thumb and little finger domains, which are conformation-dependent protein-protein interactions that diversify the binding mode of Dpo4 on PCNA. The structure reveals a molecular model in which substrate/partner binding-coupled multiple conformations of a Y-family polymerase facilitate its recruitment and co-ordination on the sliding clamp. The conformational flexibility would turn the error-prone Y-family polymerase off when more efficient high-fidelity DNA polymerases work on undamaged DNA and turn it onto DNA templates to perform translesion synthesis when replication forks are stalled by DNA lesions.
Xing, G.; Kirouac, K.; Shin, Y.J.; Bell, S.D.; Ling, H.; (UWO); (Sir W.Dunn)
IMPORTANCE OF NEUTRINOLESS DOUBLE BETA DECAY Abstract
number violation in nature and what is its magnitude. The neutrinoless double beta decay experiment can
U. Sarkar; Utpal Sarkar
Double Hot Springs Geothermal Area | Open Energy Information
Open Energy Info (EERE)
AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 NoPublic Utilities Address:011-DNA Jump to:52c8ff988c1DeringDolgeville, NewDorchester, Wisconsin: EnergyDorsettDouble
Conformational plasticity of the influenza A M2 transmembrane helix in lipid bilayers under varying pH, drug binding, and membrane thickness
Conformational plasticity of the influenza A M2 transmembrane helix in lipid bilayers under varying cues, thus conformational plasticity is important for function. The influenza A M2 protein forms spectroscopy to examine the conformational plasticity of membrane-bound transmembrane domain of M2 (M2TM). 13 C
Hong, Mei
Predicting Neutrinoless Double Beta Decay
We give predictions for the neutrinoless double beta decay rate in a simple variant of the A_4 family symmetry model. We show that there is a lower bound for the neutrinoless double beta decay amplitude even in the case of normal hierarchical neutrino masses, corresponding to an effective mass parameter |m_{ee}| >= 0.17 \\sqrt{\\Delta m^2_{ATM}}. This result holds both for the CP conserving and CP violating cases. In the latter case we show explicitly that the lower bound on |m_{ee}| is sensitive to the value of the Majorana phase. We conclude therefore that in our scheme, neutrinoless double beta decay may be accessible to the next generation of high sensitivity experiments.
M. Hirsch; Ernest Ma; J. W. F. Valle; A. Villanova del Moral
A Speculation into the Origin of Neutral Globules In Planetary Nebulae: Could the Helix's Comets Really Be Comets?
A novel explanation for the origin of the cometary globules within NGC 7293 (the "Helix" planetary nebula) is examined; that these globules originate as massive cometary bodies at large astrocentric radii. The mass of such hypothetical cometary bodies would have to be several orders of magnitude larger than any such bodies observed in our solar system in order to supply the observed mass of neutral gas. It is however shown that comets at "outer Oort cloud" like distances are likely to survive past the red giant and asymptotic giant branch evolutionary phases of the central star, allowing them to survive until the formation of the planetary nebula. Some observational tests of this hypothesis are proposed.
Grant Gussie
DNA Sequencing apparatus
An automated DNA sequencing apparatus having a reactor for providing at least two series of DNA products formed from a single primer and a DNA strand, each DNA product of a series differing in molecular weight and having a chain terminating agent at one end; separating means for separating the DNA products to form a series bands, the intensity of substantially all nearby bands in a different series being different, band reading means for determining the position an This invention was made with government support including a grant from the U.S. Public Health Service, contract number AI-06045. The U.S. government has certain rights in the invention.
Tabor, Stanley (Cambridge, MA); Richardson, Charles C. (Chestnut Hill, MA)
Neutrinoless Double Beta Decay Constraints
A brief overview is given of theoretical analyses with neutrinoless double beta decay experiments. Theoretical bounds on the ``observable'', _betabeta, are presented. By using experimental bounds on _betabeta, allowed regions are obtained on the m_l-cos{2theta_12} plane, where m_l stands for the lightest neutrino mass. It is shown that Majorana neutrinos can be excluded by combining possible results of future neutrinoless double beta decay and {}^3H beta decay experiments. A possibility to constrain one of two Majorana phases is discussed also.
Hiroaki Sugiyama
Introduction to Double Beta Decay
While the neutrino oscillation experiments have measured non-zero square mass differences between neutrino flavors, the double beta decay is the most sensitive process to the absolute mass of the neutrinos and the only that can distinguish whether neutrino is a Dirac or a Majorana particle. These are a few but not the only reasons that many efforts, both theoretically and experimentally, are dedicated to the study of this process. In this lecture I'll give a short introduction to the double beta decay process. Different decay modes and mechanisms, a short history, decay rates and extraction of the neutrino mass parameter are presented.
Stoica, Sabin [Horia Hulubei National Institute for Physics and Nuclear Engineering, P.O. Box MG-6, 077125 Magurele (Romania); Horia Hulubei Foundation, 407, Atomistilor street, 077125 Magurele (Romania)
Double beta decay: present status
The present status of double beta decay experiments (including the search for $2\\beta^{+}$, EC$\\beta^{+}$ and ECEC processes) are reviewed. The results of the most sensitive experiments are discussed. Average and recommended half-life values for two-neutrino double beta decay are presented. Conservative upper limits on effective Majorana neutrino mass and the coupling constant of the Majoron to the neutrino are established as $ beta decay experiments with a sensitivity for the $$ at the level of (0.01-0.1) eV are considered.
Attosecond Double-Slit Experiment
A new scheme for a double-slit experiment in the time domain is presented. Phase-stabilized few-cycle laser pulses open one to two windows (slits) of attosecond duration for photoionization. Fringes in the angle-resolved energy spectrum of varying visibility depending on the degree of which-way information are measured. A situation in which one and the same electron encounters a single and a double slit at the same time is observed. The investigation of the fringes makes possible interferometry on the attosecond time scale. From the number of visible fringes, for example, one derives that the slits are extended over about 500 as.
Lindner, F.; Schaetzel, M.G.; Baltuska, A.; Goulielmakis, E. [Max-Planck-Institut fuer Quantenoptik, 85748 Garching (Germany); Walther, H. [Max-Planck-Institut fuer Quantenoptik, 85748 Garching (Germany); Ludwig-Maximilians-Universitaet Muenchen, 85748 Garching (Germany); Krausz, F. [Max-Planck-Institut fuer Quantenoptik, 85748 Garching (Germany); Ludwig-Maximilians-Universitaet Muenchen, 85748 Garching (Germany); Institut fuer Photonik, Technische Universitaet Wien, Gusshausstr. 27, A-1040 Vienna (Austria); Milosevic, D.B. [Faculty of Science, University of Sarajevo, Zmaja od Bosne 35, 71000 Sarajevo (Bosnia and Herzegowina); Bauer, D. [Max-Planck-Institut fuer Kernphysik, Saupfercheckweg 1, 69117 Heidelberg (Germany); Becker, W. [Max-Born-Institut, Max-Born-Str. 2a, 12489 Berlin (Germany); Paulus, G.G. [Max-Planck-Institut fuer Quantenoptik, 85748 Garching (Germany); Ludwig-Maximilians-Universitaet Muenchen, 85748 Garching (Germany); Department of Physics, Texas A and M University, College Station, Texas 77843-4242 (United States)
Neutrinoless double beta decay experiments
The study of neutrinoless double beta decay is of outmost importance for neutrino physics. It is considered to be the gold plated channel to probe the fundamental character of neutrinos and to determine the neutrino mass. From the experimental point about nine different isotopes are explored for the search. After a general introduction follows a short discussion on nuclear matrix element calculations and supportive measurements. The current experimental status of double beta searches is presented followed by a short discussion of the ideas and proposals for large scale experiments.
An unprecedented nucleic acid capture mechanism for excision of DNA damage
DNA glycosylases that remove alkylated and deaminated purine nucleobases are essential DNA repair enzymes that protect the genome, and at the same time confound cancer alkylation therapy, by excising cytotoxic N3-methyladenine bases formed by DNA-targeting anticancer compounds. The basis for glycosylase specificity towards N3- and N7-alkylpurines is believed to result from intrinsic instability of the modified bases and not from direct enzyme functional group chemistry. Here we present crystal structures of the recently discovered Bacillus cereus AlkD glycosylase in complex with DNAs containing alkylated, mismatched and abasic nucleotides. Unlike other glycosylases, AlkD captures the extrahelical lesion in a solvent-exposed orientation, providing an illustration for how hydrolysis of N3- and N7-alkylated bases may be facilitated by increased lifetime out of the DNA helix. The structures and supporting biochemical analysis of base flipping and catalysis reveal how the HEAT repeats of AlkD distort the DNA backbone to detect non-Watson-Crick base pairs without duplex intercalation.
Rubinson, Emily H.; Prakasha Gowda, A.S.; Spratt, Thomas E.; Gold, Barry; Eichmanbrand, Brandt F. (Pitt); (Vanderbilt); (Penn)
Constraining neutrinoless double beta decay
A class of discrete flavor-symmetry-based models predicts constrained neutrino mass matrix schemes that lead to specific neutrino mass sum-rules (MSR). We show how these theories may constrain the absolute scale of neutrino mass, leading in most of the cases to a lower bound on the neutrinoless double beta decay effective amplitude.
L. Dorame; D. Meloni; S. Morisi; E. Peinado; J. W. F. Valle
Detection and quantitation of single nucleotide polymorphisms, DNA sequence variations, DNA mutations, DNA damage and DNA mismatches
DNA mutation binding proteins alone and as chimeric proteins with nucleases are used with solid supports to detect DNA sequence variations, DNA mutations and single nucleotide polymorphisms. The solid supports may be flow cytometry beads, DNA chips, glass slides or DNA dips sticks. DNA molecules are coupled to solid supports to form DNA-support complexes. Labeled DNA is used with unlabeled DNA mutation binding proteins such at TthMutS to detect DNA sequence variations, DNA mutations and single nucleotide length polymorphisms by binding which gives an increase in signal. Unlabeled DNA is utilized with labeled chimeras to detect DNA sequence variations, DNA mutations and single nucleotide length polymorphisms by nuclease activity of the chimera which gives a decrease in signal.
McCutchen-Maloney, Sandra L. (Pleasanton, CA)
A Search for Neutrinoless Double Beta Decay of Te-130
far unobserved, neutrinoless double beta decay is a possibleright for the neutrinoless double beta decay of 130 Te. Thisprocess, with neutrinoless double beta decay being the most
Bryant, Adam Douglas
What can we learn from neutrinoless double beta decay experiments?
Limits From Neutrinoless Double-Beta Decay (Rev. ),� ina next generation neutrinoless double beta decay search andPARTICLES? NO NEUTRINOLESS DOUBLE BETA DECAY AND INVERTED
Bahcall, John N.
subsequently published arguments, non-observation of neutrinoless double beta decay has, to date, no bearing on
Clemens A. Heusch; Peter Minkowski
Neutrinoless Double Beta Decay and CP Violation
neutrinoless double beta decay in the case of two neutrino generations (or when the third generation leptons do
Patrick J. O�donnell; Utpal Sarkar
Neutrinoless Double Beta Decay Experiments
Neutrinoless double beta decay is the only process known so far able to test the neutrino intrinsic nature: its experimental observation would imply that the lepton number is violated by two units and prove that neutrinos have a Majorana mass components, being their own anti-particle. While several experiments searching for such a rare decay have been performed in the past, a new generation of experiments using different isotopes and techniques have recently released their results or are taking data and will provide new limits, should no signal be observed, in the next few years to come. The present contribution reviews the latest public results on double beta decay searches and gives an overview on the expected sensitivities of the experiments in construction which will be able to set stronger limits in the near future.
Alberto Garfagnini
Garfagnini, Alberto
Pair extended coupled cluster doubles
The accurate and efficient description of strongly correlated systems remains an important challenge for computational methods. Doubly occupied configuration interaction (DOCI), in which all electrons are paired and no correlations which break these pairs are permitted, can in many cases provide an accurate account of strong correlations, albeit at combinatorial computational cost. Recently, there has been significant interest in a method we refer to as pair coupled cluster doubles (pCCD), a variant of coupled cluster doubles in which the electrons are paired. This is simply because pCCD provides energies nearly identical to those of DOCI, but at mean-field computational cost (disregarding the cost of the two-electron integral transformation). Here, we introduce the more complete pair extended coupled cluster doubles (pECCD) approach which, like pCCD, has mean-field cost and reproduces DOCI energetically. We show that unlike pCCD, pECCD also reproduces the DOCI wave function with high accuracy. Moreoever, pEC...
Henderson, Thomas M; Scuseria, Gustavo E
An autoinhibitory helix in the C-terminal region of phospholipase C-[beta] mediates G[alpaha subscript q] activation
The enzyme phospholipase C-{beta} (PLC{beta}) is a crucial regulator of intracellular calcium levels whose activity is controlled by heptahelical receptors that couple to members of the G{sub q} family of heterotrimeric G proteins. We have determined atomic structures of two invertebrate homologs of PLC{beta} (PLC21) from cephalopod retina and identified a helix from the C-terminal regulatory region that interacts with a conserved surface of the catalytic core of the enzyme. Mutations designed to disrupt the analogous interaction in human PLC{beta}3 considerably increase basal activity and diminish stimulation by G{alpha}{sub q}. G{alpha}{sub q} binding requires displacement of the autoinhibitory helix from the catalytic core, thus providing an allosteric mechanism for activation of PLC{beta}.
Lyon, Angeline M.; Tesmer, Valerie M.; Dhamsania, Vishan D.; Thal, David M.; Gutierrez, Joanne; Chowdhury, Shoaib; Suddala, Krishna C.; Northup, John K.; Tesmer, John J.G. (Michigan); (NIH)
Radiation can damage cellular components, including DNA. Organisms have developed a panoply of means of dealing with DNA damage. Some repair paths have rather narrow substrate specificity (e.g. photolyases), which act on specific pyrimidine photoproducts in a specific type (e.g., DNA) and conformation (double-stranded B conformation) of nucleic acid. Others, for example, nucleotide excision repair, deal with larger classes of damages, in this case bulky adducts in DNA. A detailed discussion of DNA repair mechanisms is beyond the scope of this article, but one can be found in the excellent book of Friedberg et al. [1] for further detail. However, some DNA damages and paths for repair of those damages important for photobiology will be outlined below as a basis for the specific examples of genetic and molecular analysis that will be presented below.
Supramolecular DNA nanotechnology : discrete nanoparticle organization, three-dimensional DNA construction, and molecule templated DNA assembly.
??The field of structural DNA nanotechnology utilizes DNA's powerful base-pairing molecular recognition criteria to help solve real challenges facing researchers in material science and nanotechnology,� (more)
Aldaye, Faisal A., 1979-
Investigations of ?-helix??-sheet transition pathways in a miniprotein using the finite-temperature string method
A parallel implementation of the finite-temperature string method is described, which takes into account the invariance of coordinates with respect to rigid-body motions. The method is applied to the complex ?-helix??-sheet transition in a ?-hairpin miniprotein in implicit solvent, which exhibits much of the complexity of conformational changes in proteins. Two transition paths are considered, one derived from a linear interpolant between the endpoint structures and the other derived from a targeted dynamics simulation. Two methods for computing the conformational free energy (FE) along the string are compared, a restrained method, and a tessellation method introduced by E. Vanden-Eijnden and M. Venturoli [J. Chem. Phys. 130, 194103 (2009)]. It is found that obtaining meaningful free energy profiles using the present atom-based coordinates requires restricting sampling to a vicinity of the converged path, where the hyperplanar approximation to the isocommittor surface is sufficiently accurate. This sampling restriction can be easily achieved using restraints or constraints. The endpoint FE differences computed from the FE profiles are validated by comparison with previous calculations using a path-independent confinement method. The FE profiles are decomposed into the enthalpic and entropic contributions, and it is shown that the entropy difference contribution can be as large as 10 kcal/mol for intermediate regions along the path, compared to 15�20 kcal/mol for the enthalpy contribution. This result demonstrates that enthalpic barriers for transitions are offset by entropic contributions arising from the existence of different paths across a barrier. The possibility of using systematically coarse-grained representations of amino acids, in the spirit of multiple interaction site residue models, is proposed as a means to avoid ad hoc sampling restrictions to narrow transition tubes.
Ovchinnikov, Victor, E-mail: firstname.lastname@example.com [Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138 (United States)] [Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138 (United States); Karplus, Martin, E-mail: email@example.com [Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138 (United States) [Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts 02138 (United States); Laboratoire de Chimie Biophysique, ISIS, Universit� de Strasbourg, 67000 Strasbourg (France)
ap2-integrase dna binding: Topics by E-print Network
Ser-142 (14). Ser-129 is believed to be a substrate for glycogen synthase kinase 3 Brownfield, Mark S. 156 An Unusual Helix Turn Helix Motif in the Catalytic Core of HIV-1...
Review of double beta decay experiments
The brief review of current experiments on search and studying of double beta decay processes is done. Best present limits on $\\langle m_{\
Operator Analysis of Neutrinoless Double Beta Decay
We study the effective operators of the standard model fields which would yield an observable rate of neutrinoless double beta decay. We particularly focus on the possibility that neutrinoless double beta decay is dominantly induced by lepton-number-violating higher dimensional operators other than the Majorana neutrino mass. Our analysis can be applied to models in which neutrinoless double beta decay is induced either by a strong dynamics or by quantum gravity effects at a fundamental scale near the TeV scale as well as the conventional models in which neutrinoless double beta decay is induced by perturbative renormalizable interactions.
Kiwoon Choi; Kwang Sik Jeong; Wan Young Song
Double perovskite catalysts for oxidative coupling
Alkali metal doped double perovskites containing manganese and at least one of cobalt, iron and nickel are useful in the oxidative coupling of alkane to higher hydrocarbons.
Campbell, K.D.
Neutrinoless Double Beta Decay: Present and Future
Present status, and future plans for Double Beta Decay searches are reviewed. Given the recent observations of neutrino oscillations, a possibility to observe $\\beta\\beta(0\
Oliviero Cremonesi
Barabash, A S
Double Flash | Open Energy Information
AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE: Alternative Fuels Data Center Home Page on Google Bookmark EERE: Alternative Fuels Data Center Home5b9fcbce19 NoPublic Utilities Address:011-DNA Jump to:52c8ff988c1DeringDolgeville, NewDorchester, Wisconsin: EnergyDorsett
Electrophoretic detection and separation of mutant DNA using replaceable polymer matrices
The disclosure relates to a method for resolving double-stranded DNA species differing by at least one base pair. Each of the species is characterized by an iso-melting domain with a unique melting temperature contiguous with a melting domain of higher thermal stability. 18 figs.
Karger, B.L.; Thilly, W.G.; Foret, F.; Khrapko, K.; Koehavong, P.; Cohen, A.S.; Giese, R.W.
Divalent Ion and Thermally Induced DNA Conformational Polymorphism on Single-walled
coverage on nanotubes dispersed in aqueous solution decreases, the emission energy decreases (due Nanotubes Hong Jin1 , Esther S. Jeng1 , Daniel A. Heller2 , Prakrit V. Jena3 , Robert Kirmse4 , Jörg and double stranded DNA can wrap and colloidally stabilize single walled carbon nanotubes in solution
Langowski, Jörg
The disclosure relates to a method for resolving double-stranded DNA species differing by at least one base pair. Each of the species is characterized by an iso-melting domain with a unique melting temperature contiguous with a melting domain of higher thermal stability.
Karger, Barry L. (Newton, MA); Thilly, William G. (Winchester, MA); Foret, Frantisek (Malden, MA); Khrapko, Konstaintin (Brookline, MA); Koehavong, Phouthone (Pittsburgh, PA); Cohen, Aharon S. (Newton, MA); Giese, Roger W. (Quincy, MA)
Strandwise translocation of a DNA glycosylase on undamaged DNA
Base excision repair of genotoxic nucleobase lesions in the genome is critically dependent upon the ability of DNA glycosylases to locate rare sites of damage embedded in a vast excess of undamaged DNA, using only thermal energy to fuel the search process. Considerable interest surrounds the question of how DNA glycosylases translocate efficiently along DNA while maintaining their vigilance for target damaged sites. Here, we report the observation of strandwise translocation of 8-oxoguanine DNA glycosylase, MutM, along undamaged DNA. In these complexes, the protein is observed to translocate by one nucleotide on one strand while remaining untranslocated on the complementary strand. We further report that alterations of single base-pairs or a single amino acid substitution (R112A) can induce strandwise translocation. Molecular dynamics simulations confirm that MutM can translocate along DNA in a strandwise fashion. These observations reveal a previously unobserved mode of movement for a DNA-binding protein along the surface of DNA.
Qi, Yan; Nam, Kwangho; Spong, Marie C.; Banerjee, Anirban; Sung, Rou-Jia; Zhang, Michael; Karplus, Martin; Verdine, Gregory L. (Harvard)
ExpandplusCrystal Structures of Poly(ADP-ribose) Polymerase-1 (PARP-1) Zinc Fingers Bound to DNA
Poly(ADP-ribose) polymerase-1 (PARP-1) has two homologous zinc finger domains, Zn1 and Zn2, that bind to a variety of DNA structures to stimulate poly(ADP-ribose) synthesis activity and to mediate PARP-1 interaction with chromatin. The structural basis for interaction with DNA is unknown, which limits our understanding of PARP-1 regulation and involvement in DNA repair and transcription. Here, we have determined crystal structures for the individual Zn1 and Zn2 domains in complex with a DNA double strand break, providing the first views of PARP-1 zinc fingers bound to DNA. The Zn1-DNA and Zn2-DNA structures establish a novel, bipartite mode of sequence-independent DNA interaction that engages a continuous region of the phosphodiester backbone and the hydrophobic faces of exposed nucleotide bases. Biochemical and cell biological analysis indicate that the Zn1 and Zn2 domains perform distinct functions. The Zn2 domain exhibits high binding affinity to DNA compared with the Zn1 domain. However, the Zn1 domain is essential for DNA-dependent PARP-1 activity in vitro and in vivo, whereas the Zn2 domain is not strictly required. Structural differences between the Zn1-DNA and Zn2-DNA complexes, combined with mutational and structural analysis, indicate that a specialized region of the Zn1 domain is re-configured through the hydrophobic interaction with exposed nucleotide bases to initiate PARP-1 activation.
M Langelier; J Planck; S Roy; J Pascal
DNA heats up : Energetics of genome ejection from phage revealed by isothermal titration calorimetry
Most bacteriophages are known to inject their double-stranded DNA into bacteria upon receptor binding in an essentially spontaneous way. This downhill thermodynamic process from the intact virion toward the empty viral capsid plus released DNA is made possible by the energy stored during active packaging of the genome into the capsid. Only indirect measurements of this energy have been available until now using either single-molecule or osmotic suppression techniques. In this paper, we describe for the first time the use of isothermal titration calorimetry to directly measure the heat released (or equivalently the enthalpy) during DNA ejection from phage lambda, triggered in solution by a solubilized receptor. Quantitative analyses of the results lead to the identification of thermodynamic determinants associated with DNA ejection. The values obtained were found to be consistent with those previously predicted by analytical models and numerical simulations. Moreover, the results confirm the role of DNA hydration in the energetics of genome confinement in viral capsids.
Meerim Jeembaeva; B. J�nsson; Martin Castelnovo; Alex Evilevitch
Coarse-graining DNA for simulations of DNA nanotechnology
To simulate long time and length scale processes involving DNA it is necessary to use a coarse-grained description. Here we provide an overview of different approaches to such coarse graining, focussing on those at the nucleotide level that allow the self-assembly processes associated with DNA nanotechnology to be studied. OxDNA, our recently-developed coarse-grained DNA model, is particularly suited to this task, and has opened up this field to systematic study by simulations. We illustrate some of the range of DNA nanotechnology systems to which the model is being applied, as well as the insights it can provide into fundamental biophysical properties of DNA.
Doye, Jonathan P K; Louis, Ard A; Romano, Flavio; Sulc, Petr; Matek, Christian; Snodin, Benedict E K; Rovigatti, Lorenzo; Schreck, John S; Harrison, Ryan M; Smith, William P J
Double bevel construction of a diamond anvil
A double or multiple bevel culet geometry is used on a diamond anvil in a high pressure cell apparatus to provide increased sample pressure and stability for a given force applied to the diamond tables. Double or multiple bevel culet geometries can also be used for sapphire or other hard crystal anvils. Pressures up to and above 5 Megabars can be reached. 8 figs.
Moss, W.C.
Double beta decay: experiments and theory review
Neutrinoless double beta decay is one of the most powerful tools to set the neutrino mass absolute scale and establish whether the neutrino is a Majorana particle. After a summary of the neutrinoless double beta decay phenomenology, the present status of the experimental search for this rare decay is reported and the prospects for next generation experiments are reviewed.
A. Nucciotti
Neutrinoless double beta decay and neutrino physics
The connection of neutrino physics with neutrinoless double beta decay is reviewed. After presenting the current status of the PMNS matrix and the theoretical background of neutrino mass and lepton mixing, we will summarize the various implications of neutrino physics for double beta decay. The influence of light sterile neutrinos and other exotic modifications of the three neutrino picture is also discussed.
Werner Rodejohann
Reliability Estimation for Double Containment Piping
Double walled or double containment piping is considered for use in the ITER international project and other next-generation fusion device designs to provide an extra barrier for tritium gas and other radioactive materials. The extra barrier improves confinement of these materials and enhances safety of the facility. This paper describes some of the design challenges in designing double containment piping systems. There is also a brief review of a few operating experiences of double walled piping used with hazardous chemicals in different industries. This paper recommends approaches for the reliability analyst to use to quantify leakage from a double containment piping system in conceptual and more advanced designs. The paper also cites quantitative data that can be used to support such reliability analyses.
L. Cadwallader; T. Pinna
Instability limits for spontaneous double layer formation
We present time-resolved measurements that demonstrate that large amplitude electrostatic instabilities appear in pulsed, expanding helicon plasmas at the same time as particularly strong double layers appear in the expansion region. A significant cross-correlation between the electrostatic fluctuations and fluctuations in the number of ions accelerated by the double layer electric field is observed. No correlation is observed between the electrostatic fluctuations and ions that have not passed through the double layer. These measurements confirm that the simultaneous appearance of the electrostatic fluctuations and the double layer is not simple coincidence. In fact, the accelerated ion population is responsible for the growth of the instability. The double layer strength, and therefore, the velocity of the accelerated ions, is limited by the appearance of the electrostatic instability.
Carr, J. Jr. [Department of Physics, West Virginia University, Morgantown, West Virginia 26506 (United States) [Department of Physics, West Virginia University, Morgantown, West Virginia 26506 (United States); Department of Physics, Texas Lutheran University, Seguin, Texas 78155 (United States); Galante, M. E.; McCarren, D.; Scime, E. E.; Sears, S.; VanDervort, R. W. [Department of Physics, West Virginia University, Morgantown, West Virginia 26506 (United States)] [Department of Physics, West Virginia University, Morgantown, West Virginia 26506 (United States); Magee, R. M. [Department of Physics, West Virginia University, Morgantown, West Virginia 26506 (United States) [Department of Physics, West Virginia University, Morgantown, West Virginia 26506 (United States); TriAlpha Energy, Inc., Foothill Ranch, California 92610 (United States); Reynolds, E. [Department of Physics and Engineering, West Virginia Wesleyan, Buckhannon, West Virginia 26201 (United States)] [Department of Physics and Engineering, West Virginia Wesleyan, Buckhannon, West Virginia 26201 (United States)
Plectoneme tip bubbles: Coupled denaturation and writhing in supercoiled DNA
Biological information is not only stored in the digital chemical sequence of double helical DNA, but is also encoded in the mechanical properties of the DNA strands, which can influence biochemical processes involving its readout. For example, loop formation in the Lac operon can regulate the expression of key genes, and DNA supercoiling is closely correlated to rhythmic circardian gene expression in cyanobacteria. Supercoiling is also important for large scale organisation of the genome in both eukaryotic and prokaryotic cells. DNA can respond to torsional stress by writhing to form looped structures called plectonemes, thus transferring energy stored as twist into energy stored in bending. Denaturation bubbles can also relax torsional stress, with the enthalpic cost of breaking bonds being compensated by their ability to absorb undertwist. Here we predict a novel regime where bubbles form at the tips of plectonemes, and study its properties using coarse-grained simulations. These tip bubbles can occur for both positive and negative supercoiling and greatly reduce plectoneme diffusion by a pinning mechanism. They can cause plectonemes to preferentially localise to AT rich regions, because bubbles more easily form there. The tip-bubble regime occurs for supercoiling densities and forces that are typically encountered for DNA in vivo, and may be exploited for biological control of genomic processes.
Christian Matek; Thomas E. Ouldridge; Jonathan P. K. Doye; Ard A. Louis
DNA polymerase having modified nucleotide binding site for DNA sequencing
A modified gene encoding a modified DNA polymerase is disclosed. The modified polymerase incorporates dideoxynucleotides at least 20-fold better compared to the corresponding deoxynucleotides as compared with the corresponding naturally-occurring DNA polymerase. 6 figs.
Tabor, S.; Richardson, C.
Neutrinoless Double Beta Decay in Light of SNO Salt Data
Limits From Neutrinoless Double-Beta Decay (Rev. ),� incan also cause neutrinoless double-beta decay (see e.g. , [LBNL-53996 Neutrinoless Double Beta Decay in Light of SNO
Murayama, Hitoshi
312 P. Vogel, �Limits From Neutrinoless Double-Beta Decay (Assumptions No detected neutrinoless double ?-decay lightestscale (1 � 0.05 eV), No neutrinoless double ?-decay lightest
Bolometric experiments for neutrinoless double beta 3.2.1A Search for Neutrinoless Double Beta Decay of Te by AdamSpring 2010 A Search for Neutrinoless Double Beta Decay of
14] P. Vogel, �Limits From Neutrinoless Double-Beta Decay (Higgs, can also cause neutrinoless double-beta decay (seeLBNL-53996 Neutrinoless Double Beta Decay in Light of SNO
Smectic Order in Double-Twist Cylinders
I propose a double-twist texture with local smectic order, which may have been seen in recent experiments. As in the Renn-Lubensky TGB phase, the smectic order is broken only through a lattice of screw dislocations. A melted lattice of screw dislocations can produce a double-twist texture as can an unmelted lattice. In the latter case I show that geometry only allows for certain angles between smectic regions. I discuss the possibility of connecting these double-twist tubes together to form a smectic blue phase.
Randall D. Kamien
Neutrinoless Double Beta Decay and Neutrino Masses
Neutrinoless double beta decay is a promising test for lepton number violating physics beyond the standard model of particle physics. There is a deep connection between this decay and the phenomenon of neutrino masses. In particular, we will discuss the relation between neutrinoless double beta decay and Majorana neutrino masses provided by the so-called Schechter--Valle theorem in a quantitative way. Furthermore, we will present an experimental cross check to discriminate neutrinoless double beta decay from unknown nuclear background using only one isotope, i.e., within one experiment.
Michael Duerr
albicans double infection: Topics by E-print Network
Summary: Scintillator detectors can be used in experiments searching for neutrinoless double beta decay. A wide variety of double beta decay candidate isotopes can be made into...
National CHP Roadmap: Doubling Combined Heat and Power Capacity...
Broader source: Energy.gov (indexed) [DOE]
National CHP Roadmap: Doubling Combined Heat and Power Capacity in the United States by 2010, March 2001 National CHP Roadmap: Doubling Combined Heat and Power Capacity in the...
Dynamic Phase Shifts in Nanoscale Distance Measurements by Double...
by Double Electron Electron Resonance (DEER)� . Abstract: The off-resonant pump pulse used in double electron electron resonance (DEER) measurements produces dynamic...
DNA Structural Nanotechnology Duke University
DNA Structural Nanotechnology John Reif Duke University Graduate Students: Harish Chandran&Caltech Tube Lattices #12;Ned Seeman New York University, USA Ned Seeman: Father of DNA Nanotechnology His Initial Ideas & Motivation for DNA Nanotechnology #12;Cube Chen & Seeman, Nature350:631 (1991) Truncated
Reif, John H.
Double layer capacitors : automotive applications and modeling
This thesis documents the work on the modeling of double layer capacitors (DLCs) and the validation of the modeling procedure. Several experiments were conducted to subject the device under test to a variety of ...
New, David Allen, 1976-
CP Violation in Neutrinoless Double Beta Decay
We argue three-flavour neutrino mixing. We consider the neutrinos as Majorana particles and see how the neutrinoless double beta decay constrains the neutrino mixing angles. Our formulation is widely valid and is applied to the neutrino oscillation experiment.
T. Fukuyama; K. Matsuda; H. Nishiura
Phenomenology of single and double diffraction dissociation
Predictions of the gap-probability renormalization model for single and double diffraction dissociation cross sections in proton-proton collisions at the LHC are presented and compared with recent CMS measurements.
Konstantin Goulianos
Phenomenology of neutrinoless double beta decay
This paper reviews the current status and future outlook of neutrinoless double beta decay searches, which try to provide an answer to the fundamental question of whether neutrinos are Dirac or Majorana particles.
G�mez-Cadenas, J J
J. J. G�mez-Cadenas; Justo Mart�n-Albo
Use of double or multiple bevel culet geometry on a diamond anvil to provide increased sample pressure and stability for a given force applied to the diamond tables. 7 figs.
We study the relation between the Majorana neutrino mass matrices and the neutrinoless double beta decay when CP is not conserved. We give an explicit form of the decay rate in terms of a rephasing invariant quantity and demonstrate that in the presence of CP violation it is impossible to have vanishing neutrinoless double beta decay in the case of two neutrino generations (or when the third generation leptons do not mix with other leptons and hence decouple).
Patrick J. O'Donnell; Utpal Sarkar
Neutrinoless double beta decay with scalar bilinears
One possible probe to physics beyond the standard model is to look for scalar bilinears, which couple to two fermions of the standard model. We point out that the scalar bilinears allow new diagrams contributing to the neutrinoless double beta decay. The upper bound on the neutrinoless double beta decay lifetime would then give new constraints on the ratio of the masses of these scalars to their couplings to the fermions.
H. V. Klapdor-Kleingrothaus; U. Sarkar
Wavelength-doubling optical parametric oscillator
A wavelength-doubling optical parametric oscillator (OPO) comprising a type II nonlinear optical medium for generating a pair of degenerate waves at twice a pump wavelength and a plurality of mirrors for rotating the polarization of one wave by 90 degrees to produce a wavelength-doubled beam with an increased output energy by coupling both of the degenerate waves out of the OPO cavity through the same output coupler following polarization rotation of one of the degenerate waves.
Armstrong, Darrell J. (Albuquerque, NM); Smith, Arlee V. (Albuquerque, NM)
Searches for neutrinoless double beta decay
Neutrinoless double beta decay is a lepton number violating process whose observation would also establish that neutrinos are their own anti-particles. There are many experimental efforts with a variety of techniques. Some (EXO, Kamland-Zen, GERDA phase I and CANDLES) started take data in 2011 and EXO has reported the first measurement of the half life for the double beta decay with two neutrinos of $^{136}$Xe. The sensitivities of the different proposals are reviewed.
B. Schwingenheuer
Double shell tank waste analysis plan
Waste analysis plan for the double shell tanks. SD-WM-EV-053 is Superseding SD-WM-EV-057.This document provides the plan for obtaining information needed for the safe waste handling and storage of waste in the Double Shell Tank Systems. In Particular it addresses analysis necessary to manage waste according to Washington Administrative Code 173-303 and Title 40, parts 264 and 265 of the Code of Federal Regulations.
Mulkey, C.H.; Jones, J.M.
The effect of magnesium ions on dielectric relaxation in semidilute DNA aqueous solutions
The effect of magnesium ion Mg2+ on the dielectric relaxation of semidilute DNA aqueous solutions has been studied by means of dielectric spectroscopy. Two dielectric relaxations in the 100 Hz - 100 MHz frequency range, originating in the motion of DNA counterions, were probed as a function of DNA and Mg2+ ion concentration in added MgCl2 salt. The high-frequency mode in the MHz range, stemming from the structural organization of the DNA network, reveals de Gennes-Pfeuty-Dobrynin correlation length as the pertinent fundamental length scale for sufficiently low concentration of added salt. No relaxation fingerprint of DNA denaturation bubbles, leading to exposed hydrophobic core scaling, was detected at low DNA concentrations, thus indicating an increased stability of the double-stranded conformation as compared to the case of DNA solutions with univalent counterions. The presence of Mg2+ does not change qualitatively the low frequency mode in the kHz range correlated with single DNA conformational properties....
Grgi?in, Danijel; Ivek, Tomislav; Tomi?, Silvia; Podgornik, Rudi
Fleet DNA (Presentation)
The Fleet DNA project objectives include capturing and quantifying drive cycle and technology variation for the multitude of medium- and heavy-duty vocations; providing a common data storage warehouse for medium- and heavy-duty vehicle fleet data across DOE activities and laboratories; and integrating existing DOE tools, models, and analyses to provide data-driven decision making capabilities. Fleet DNA advantages include: for Government - providing in-use data for standard drive cycle development, R&D, tech targets, and rule making; for OEMs - real-world usage datasets provide concrete examples of customer use profiles; for fleets - vocational datasets help illustrate how to maximize return on technology investments; for Funding Agencies - ways are revealed to optimize the impact of financial incentive offers; and for researchers -a data source is provided for modeling and simulation.
Walkokwicz, K.; Duran, A.
Chiral Mesophases of DNA
In the hexagonal columnar phase of chiral polymers a bias towards cholesteric twist competes with braiding along an average direction. When the chirality is strong, topological defects proliferate, leading to either a tilt grain boundary phase or a new ``moire state'' with twisted bond order. This moire phase can melt leading to a new phase: the chiral hexatic. I will discuss some recent experimental results from the NIH on DNA liquid crystals in the context of these theories.
DNA waves and water
Some bacterial and viral DNA sequences have been found to induce low frequency electromagnetic waves in high aqueous dilutions. This phenomenon appears to be triggered by the ambient electromagnetic background of very low frequency. We discuss this phenomenon in the framework of quantum field theory. A scheme able to account for the observations is proposed. The reported phenomenon could allow to develop highly sensitive detection systems for chronic bacterial and viral infections.
L. Montagnier; J. Aissa; E. Del Giudice; C. Lavallee; A. Tedeschi; G. Vitiello
Ortho-positronium observation in the Double Chooz Experiment
The Double Chooz experiment measures the neutrino mixing angle $\\theta_{13}$ by detecting reactor $\\bar{\
Y. Abe; J. C. dos Anjos; J. C. Barriere; E. Baussan; I. Bekman; M. Bergevin; T. J. C. Bezerra; L. Bezrukov; E. Blucher; C. Buck; J. Busenitz; A. Cabrera; E. Caden; L. Camilleri; R. Carr; M. Cerrada; P. -J. Chang; E. Chauveau; P. Chimenti; A. P. Collin; E. Conover; J. M. Conrad; J. I. Crespo-Anadon; K. Crum; A. S. Cucoanes; E. Damon; J. V. Dawson; J. Dhooghe; D. Dietrich; Z. Djurcic; M. Dracos; M. Elnimr; A. Etenko; M. Fallot; F. von Feilitzsch; J. Felde; S. M. Fernandes; V. Fischer; D. Franco; M. Franke; H. Furuta; I. Gil-Botella; L. Giot; M. Goger-Neff; L. F. G. Gonzalez; L. Goodenough; M. C. Goodman; C. Grant; N. Haag; T. Hara; J. Haser; M. Hofmann; G. A. Horton-Smith; A. Hourlier; M. Ishitsuka; J. Jochum; C. Jollet; F. Kaether; L. N. Kalousis; Y. Kamyshkov; D. M. Kaplan; T. Kawasaki; E. Kemp; H. de Kerret; D. Kryn; M. Kuze; T. Lachenmaier; C. E. Lane; T. Lasserre; A. Letourneau; D. Lhuillier; H. P. Lima Jr; M. Lindner; J. M. Lopez-Castano; J. M. LoSecco; B. Lubsandorzhiev; S. Lucht; J. Maeda; C. Mariani; J. Maricic; J. Martino; T. Matsubara; G. Mention; A. Meregaglia; T. Miletic; R. Milincic; A. Minotti; Y. Nagasaka; Y. Nikitenko; P. Novella; L. Oberauer; M. Obolensky; A. Onillon; A. Osborn; C. Palomares; I. M. Pepe; S. Perasso; P. Pfahler; A. Porta; G. Pronost; J. Reichenbacher; B. Reinhold; M. Rohling; R. Roncin; S. Roth; B. Rybolt; Y. Sakamoto; R. Santorelli; A. C. Schilithz; S. Schonert; S. Schoppmann; M. H. Shaevitz; R. Sharankova; S. Shimojima; D. Shrestha; V. Sibille; V. Sinev; M. Skorokhvatov; E. Smith; J. Spitz; A. Stahl; I. Stancu; L. F. F. Stokes; M. Strait; A. Stuken; F. Suekane; S. Sukhotin; T. Sumiyoshi; Y. Sun; R. Svoboda; K. Terao; A. Tonazzo; H. H. Trinh Thi; G. Valdiviesso; N. Vassilopoulos; C. Veyssiere; M. Vivier; S. Wagner; N. Walsh; H. Watanabe; C. Wiebusch; L. Winslow; M. Wurm; G. Yang; F. Yermia; V. Zimmer
Computer code for double beta decay QRPA based calculations
. The Enriched Xenon Observatory for neutrinoless double beta decay (EXO) will search for the rare decays
Bertulani, Carlos A. - Department of Physics and Astronomy, Texas A&M University
DUPLEX: A molecular mechanics program in torsion angle space for computing structures of DNA and RNA
DUPLEX produces energy minimized structures of DNA and RNA of any base sequence for single and double strands. The smallest subunits are deoxydinucleoside monophosphates, and up to 12 residues, single or double stranded can be treated. In addition, it can incorporate NMR derived interproton distances an constraints in the minimizations. Both upper and lower bounds for these distances can be specified. The program has been designed to run on a UNICOS Cray supercomputer, but should run, albeit slowly, on a laboratory computer such as a VAX or a workstation.
Hingerty, B.E.
We study the relation between the Majorana neutrino mass matrices and the neutrinoless double beta decay when CP is not conserved. We give an explicit form of the decay rate in terms of a rephasing invariant quantity and demonstrate that in the presence of CP violation it is impossible to have vanishing neutrinoless double beta decay in the case of two neutrino generations (or when the third generation leptons do not mix with other leptons and hence decouple). UTPT-93-12 In the literature the relation between the Majorana neutrino mass matrices and the neutrinoless double beta decay has been studied extensively [1]. If the electron neutrino is a Majorana particle or a pseudo-Dirac particle (i.e., the symmetry of the mass matrix is not the symmetry of the weak interaction), then they can contribute to neutrinoless double beta decay, while if it is a Dirac particle then there is no neutrinoless double beta decay. A Dirac particle can be viewed as a combination of two Majorana particles
The search for neutrinoless double beta decay
In the last two decades the search for neutrinoless double beta decay has evolved into one of the highest priorities for understanding neutrinos and the origin of mass. The main reason for this paradigm shift has been the discovery of neutrino oscillations, which clearly established the existence of massive neutrinos. An additional motivation for conducting such searches comes from the existence of an unconfirmed, but not refuted, claim of evidence for neutrinoless double decay in $^{76}\\text{Ge}$. As a consequence, a new generation of experiments, employing different detection techniques and $\\beta\\beta$ isotopes, is being actively promoted by experimental groups across the world. In addition, nuclear theorists are making remarkable progress in the calculation of the neutrinoless double beta decay nuclear matrix elements, thus eliminating a substantial part of the theoretical uncertainties affecting the particle physics interpretation of this process. In this report, we review the main aspects of the double beta decay process and some of the most relevant experiments. The picture that emerges is one where searching for neutrinoless double beta decay is recognized to have both far-reaching theoretical implications and promising prospects for experimental observation in the near future.
J. J. Gomez-Cadenas; J. Martin-Albo; M. Mezzetto; F. Monrabal; M. Sorel
Neutrinoless double beta decay violates lepton number by two units, a positive observation therefore necessarily implies physics beyond the standard model. Here, three possible contributions to neutrinoless double beta decay are briefly reviewed: (a) The mass mechanism and its connection to neutrino oscillations; (b) Left-right symmetric models and the lower limit on the right-handed W boson mass; and (c) R-parity violating supersymmetry. In addition, the recently published ``extended black box'' theorem is briefly discussed. Combined with data from oscillation experiments this theorem provides proof that the neutrinoless double beta decay amplitude must receive a non-zero contribution from the mass mechanism, if neutrinos are indeed Majorana particles.
M. Hirsch
Double hadron leptoproduction in the nuclear medium
First measurement of double-hadron production in deep-inelastic scattering has been measured with the HERMES spectrometer at HERA using a 27.6 GeV positron beam with deuterium, nitrogen, krypton and xenon targets. The influence of the nuclear medium on the ratio of double-hadron to single-hadron yields has been investigated. Nuclear effects are clearly observed but with substantially smaller magnitude and reduced $A$-dependence compared to previously measured single-hadron multiplicity ratios. The data are in fair agreement with models based on partonic or pre-hadronic energy loss, while they seem to rule out a pure absorptive treatment of the final state interactions. Thus, the double-hadron ratio provides an additional tool for studying modifications of hadronization in nuclear matter.
Airapetian, A; Akopov, Z; Amarian, M; Andrus, A; Aschenauer, E C; Augustyniak, W; Avakian, R; Avetisian, A; Avetissian, E; Bailey, P; Belostotskii, S; Bianchi, N; Blok, H P; B�ttcher, Helmut B; Borisov, A; Borysenko, A; Br�ll, A; Bryzgalov, V; Capiluppi, M; Capitani, G P; Ciullo, G; Contalbrigo, M; Dalpiaz, P F; Deconinck, W; De Leo, R; Demey, M; De Nardo, L; De Sanctis, E; Devitsin, E G; Diefenthaler, M; Di Nezza, P; Dreschler, J; D�ren, M; Ehrenfried, M; Elalaoui-Moulay, A; Elbakian, G; Ellinghaus, F; Elschenbroich, U; Fabbri, R; Fantoni, A; Felawka, L; Frullani, S; Funel, A; Gapienko, G; Gapienko, V; Garibaldi, F; Garrow, K; Gavrilov, G; Karibian, V; Giordano, F; Grebenyuk, O; Gregor, I M; Griffioen, K; Guler, H; Hadjidakis, C; Hartig, M; Hasch, D; Hasegawa, T; Hesselink, W H A; Hillenbrand, A; Hoek, M; Holler, Y; Hommez, B; Hristova, I; Iarygin, G; Ivanilov, A; Izotov, A; Jackson, H E; Jgoun, A; Kaiser, R; Keri, T; Kinney, E; Kiselev, A; Kobayashi, T; Kopytin, M; Korotkov, V; Kozlov, V; Krauss, B; Kravchenko, P; Krivokhizhin, V G; Lagamba, L; Lapikas, L; Lenisa, P; Liebing, P; Linden-Levy, L A; Lorenzon, W; L�, J; Lu, S; Ma, B Q; Maiheu, B; Makins, N C R; Mao, Y; Marianski, B; Marukyan, H; Masoli, F; Mexner, V; Meyners, N; Michler, T; Miklukho, O; Miller, C A; Miyachi, Y; Muccifora, V; Murray, M; Nagaitsev, A; Nappi, E; Naryshkin, Yu; Negodaev, M; Nowak, Wolf-Dieter; Ohsuga, H; Osborne, A; Perez-Benito, R; Pickert, N; Raithel, M; Reggiani, D; Reimer, P E; Reischl, A; Reolon, A R; Riedl, C; Rith, K; Rosner, G; Rostomyan, A; Rubacek, L; Rubin, J; Ryckbosch, D; Salomatin, Y; Sanjiev, I; Savin, I; Sch�fer, A; Schnell, G; Sch�ler, K P; Seele, J; Seidl, R; Seitz, B; Shearer, C; Shibata, T A; Shutov, V; Sinram, K; Stancari, M; Statera, M; Steffens, E; Steijger, J J M; Stenzel, H; Stewart, J; Stinzing, F; Streit, J; Tait, P; Tanaka, H; Taroian, S P; Tchuiko, B; Terkulov, A R; Trzcinski, A; Tytgat, M; Vandenbroucke, A; Van der Nat, P B; van der Steenhoven, G; Van Haarlem, Y; Veretennikov, D; Vikhrov, V; Vogel, C; Wang, S; Ye, Y; Ye, Z; Yen, S; Zihlmann, B; Zupranski, P
Precision Muon Reconstruction in Double Chooz
We describe a muon track reconstruction algorithm for the reactor anti-neutrino experiment Double Chooz. The Double Chooz detector consists of two optically isolated volumes of liquid scintillator viewed by PMTs, and an Outer Veto above these made of crossed scintillator strips. Muons are reconstructed by their Outer Veto hit positions along with timing information from the other two detector volumes. All muons are fit under the hypothesis that they are through-going and ultrarelativistic. If the energy depositions suggest that the muon may have stopped, the reconstruction fits also for this hypothesis and chooses between the two via the relative goodness-of-fit. In the ideal case of a through-going muon intersecting the center of the detector, the resolution is ~40 mm in each transverse dimension. High quality muon reconstruction is an important tool for reducing the impact of the cosmogenic isotope background in Double Chooz.
Double Chooz collaboration; Y. Abe; J. C. dos Anjos; J. C. Barriere; E. Baussan; I. Bekman; M. Bergevin; T. J. C. Bezerra; L. Bezrukov; E. Blucher; C. Buck; J. Busenitz; A. Cabrera; E. Caden; L. Camilleri; R. Carr; M. Cerrada; P. -J. Chang; E. Chauveau; P. Chimenti; A. P. Collin; E. Conover; J. M. Conrad; J. I. Crespo-Anad�n; K. Crum; A. Cucoanes; E. Damon; J. V. Dawson; D. Dietrich; Z. Djurcic; M. Dracos; M. Elnimr; A. Etenko; M. Fallot; F. von Feilitzsch; J. Felde; S. M. Fernandes; V. Fischer; D. Franco; M. Franke; H. Furuta; I. Gil-Botella; L. Giot; M. G�ger-Neff; L. F. G. Gonzalez; L. Goodenough; M. C. Goodman; C. Grant; N. Haag; T. Hara; J. Haser; M. Hofmann; G. A. Horton-Smith; A. Hourlier; M. Ishitsuka; J. Jochum; C. Jollet; F. Kaether; L. N. Kalousis; Y. Kamyshkov; D. M. Kaplan; T. Kawasaki; E. Kemp; H. de Kerret; D. Kryn; M. Kuze; T. Lachenmaier; C. E. Lane; T. Lasserre; A. Letourneau; D. Lhuillier; H. P. Lima Jr; M. Lindner; J. M. L�pez-Casta no; J. M. LoSecco; B. Lubsandorzhiev; S. Lucht; J. Maeda; C. Mariani; J. Maricic; J. Martino; T. Matsubara; G. Mention; A. Meregaglia; T. Miletic; R. Milincic; A. Minotti; Y. Nagasaka; Y. Nikitenko; P. Novella; M. Obolensky; L. Oberauer; A. Onillon; A. Osborn; C. Palomares; I. M. Pepe; S. Perasso; P. Pfahler; A. Porta; G. Pronost; J. Reichenbacher; B. Reinhold; M. R�hling; R. Roncin; S. Roth; B. Rybolt; Y. Sakamoto; R. Santorelli; A. C. Schilithz; S. Sch�nert; S. Schoppmann; M. H. Shaevitz; R. Sharankova; S. Shimojima; V. Sibille; V. Sinev; M. Skorokhvatov; E. Smith; J. Spitz; A. Stahl; I. Stancu; L. F. F. Stokes; M. Strait; A. St�ken; F. Suekane; S. Sukhotin; T. Sumiyoshi; Y. Sun; R. Svoboda; K. Terao; A. Tonazzo; H. H. Trinh Thi; G. Valdiviesso; N. Vassilopoulos; C. Veyssiere; M. Vivier; S. Wagner; H. Watanabe; C. Wiebusch; L. Winslow; M. Wurm; G. Yang; F. Yermia; V. Zimmer
Recent Results in Neutrinoless Double Beta Decay
The search for neutrinoless double beta decay is a rich source for new physics. The observation of this decay will lead to understanding of the absolute mass scale of neutrinos, the Majorana nature of the neutrino (whether the neutrino is its own anti-particle), and lepton number violation. Double beta decay is being investigated around the world by several experiments using different candidate isotopes. There has been much progress made in experimental techniques recently such that achieving sensitivity to neutrino masses at 50 meV and below will be possible in the near future. A summary of recent results in neutrinoless double beta decay is discussed with a look toward the experimental goals for the future.
Lisa J. Kaufman
On the neutrinoless double ?{sup +}/EC decays
The neutrinoless double positron-emission/electron-capture (0??{sup +}/EC) decays are studied for the magnitudes of the involved nuclear matrix elements (NMEs). Decays to the ground state, 0{sub gs}{sup +}, and excited 0{sup +} states are discussed. The participant many-body wave functions are evaluated in the framework of the quasiparticle random-phase approximation (QRPA). Effective, G-matrix-derived nuclear forces are used in realistic single-particle model spaces. The channels ?{sup +}?{sup +}, ?{sup +}EC, and the resonant neutrinoless double electron capture (R0?ECEC) are discussed.
Suhonen, Jouni [Department of Physics, P.O. Box 35 (YFL), FI-40014 University of Jyv�skyl� (Finland)
The double-beta decay: Theoretical challenges
Neutrinoless double beta decay is a unique process that could reveal physics beyond the Standard Model of particle physics namely, if observed, it would prove that neutrinos are Majorana particles. In addition, it could provide information regarding the neutrino masses and their hierarchy, provided that reliable nuclear matrix elements can be obtained. The two neutrino double beta decay is an associate process that is allowed by the Standard Model, and it was observed for about ten nuclei. The present contribution gives a brief review of the theoretical challenges associated with these two process, emphasizing the reliable calculation of the associated nuclear matrix elements.
Horoi, Mihai [Department of Physics, Central Michigan University, Mount Pleasant, Michigan, 48859 (United States)
Detecting Double Beta Decays Using Nuclear Emulsions
Neutrino nature and absolute mass scale are major questions in particle physics which cannot be addressed by the present neutrino oscillation program. To answer these two questions, several neutrinoless double beta decay experiments are underway or planed for the near future. These experiments, mainly use bolometric techniques or gaseous counters coupled with scintillator detectors. The energy resolution is better in bolometric experiments but experiments coupling tracking with calorimetry have the advantage of observing the two electron tracks and remove many background sources. Here, we present a proposal of using nuclear emulsions to observe double beta decays. This technique has the advantage of precise tracking and vertexing even for low energy electrons.
Marcos Dracos
Line narrowing spectroscopic studies of DNA-carcinogen adducts and DNA-dye complexes
Laser-induced fluorescence line narrowing and non-line narrowing spectroscopic methods were applied to conformational studies of stable DNA adducts of the 7{beta}, 8{alpha}-dihydoxy-9{alpha}, l0{alpha}-epoxy-7,8,9, 10-tetrahydrobenzo[{alpha}]pyrene (anti-BPDE). Stereochemically distinct (+)-trans-, ({minus})-trans-, (+)-cis- and ({minus})-cis adducts of anti-BPDE bound to exocyclic amino group of the central guanine in an 11-mer oligonucleotide, exist in a mixture of conformations in frozen aqueous buffer matrices. The (+)-trans adduct adopts primarily an external conformation with a smaller fraction ( {approximately} 25 %) exists in a partially base-stacked conformation. Both cis adducts were found to be intercalated with significant {pi}-{pi} stacking interactions between the pyrenyl residues and the bases. Conformations of the trans-adduct of (+)-anti -BPDE in 11-mer oligonucleotides were studied as a function of flanking bases. In single stranded form the adduct at G{sub 2} or G{sub 3} (5 ft-flanking, base guanine) adopts a conformation with strong, interaction with the bases. In contrast, the adduct with a 5ft-flanking, thymine exists in a primarily helixexternal conformation. Similar differences were observed in the double stranded oligonucleotides. The nature of the 3ft-flanking base has little influence on the conformational equilibrium of the (+)-trans-anti BPDE-dG adduct. The formation and repair of BPDE-N{sup 2}-dG in DNA isolated from the skin of mice treated topically with benzo[{alpha}]pyrene (BP) was studied. Low-temperature fluorescence spectroscopy of the intact DNA identified the major adduct as (+)-trans-anti-BPDE-N-dG, and the minor adduct fraction consisted mainly of (+)-cis-anti-BPDE-N{sup 2}-dG.
Suh, Myungkoo
A Model for Structure and Thermodynamics of ssDNA and dsDNA Near...
Structure and Thermodynamics of ssDNA and dsDNA Near a Surface:A Coarse Grained Approach. A Model for Structure and Thermodynamics of ssDNA and dsDNA Near a Surface:A Coarse...
ORIGINAL PAPER Ionizing radiation induces DNA double-strand breaks in bystander primary
fibroblasts Mykyta V Sokolov1,2 , Lubomir B Smilenov3 , Eric J Hall3 , Igor G Panyutin1 , William M Bonner*,4
Induction of linear tracks of DNA double-strand breaks by -particle irradiation of
eukaryotic cells by exposing the cells to particles from a small Americium source (Box 1). Each particle) EQUIPMENT Americium (Am-241) source (SupplementaryMethods and SupplementaryFigs.13 online) Polyester film
Cai, Long
Screening Tool for Providers of Double-Stranded DNA - Energy Innovation
AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmosphericNuclear Security Administrationcontroller systemsBi (2) Sr (2)ScienceScientists In thePrincetonScottPortal
-or-nothing manner(7) . Bystander cells exhibit a variety of characteristics of IR-induced genomic instability
Integrated Microfluidic Electrochemical DNA Sensor
of small scale fluid flow Laminar flow Easy to predict the flow patterns Very little diffusion This can make mixing difficult Small volumes Don't need to waste expensive reagents Easy fluid control;DNA Purification The DNA will be extracted using Invitrogen Charge Switch beads. Cellular Lysis
Fygenson, Deborah Kuchnir
Double layer capacitor prospects look good
The Fourth International Seminar in Double Layer Capacitors and similar energy devices has been sponsored again by Dr. S.P. Wolsky and Dr. Nikola Marincic. The seminar was held in December 1994, at Deerfield Beach, FL. This report provides a brief description of information on supercapacitors.
Double tracks test site characterization report
This report presents the results of site characterization activities performed at the Double Tracks Test Site, located on Range 71 North, of the Nellis Air Force Range (NAFR) in southern Nevada. Site characterization activities included reviewing historical data from the Double Tracks experiment, previous site investigation efforts, and recent site characterization data. The most recent site characterization activities were conducted in support of an interim corrective action to remediate the Double Tracks Test Site to an acceptable risk to human health and the environment. Site characterization was performed using a phased approach. First, previously collected data and historical records sere compiled and reviewed. Generalized scopes of work were then prepared to fill known data gaps. Field activities were conducted and the collected data were then reviewed to determine whether data gaps were filled and whether other areas needed to be investigated. Additional field efforts were then conducted, as required, to adequately characterize the site. Characterization of the Double Tracks Test Site was conducted in accordance with the US Department of Energy`s (DOE) Streamlined Approach for Environmental Restoration (SAFER).
Neutrino oscillations and neutrinoless double beta decay
The relation between neutrino oscillation parameters and neutrinoless double beta decay is studied, assuming normal and inverse hierarchies for Majorana neutrino masses. For normal hierarchy the crucial dependence on U_{e3} is explored. The link with tritium beta decay is also briefly discussed.
D. Falcone; F. Tramontano
Neutrinoless double beta decay in seesaw models
We study the general phenomenology of neutrinoless double beta decay in seesaw models. In particular, we focus on the dependence of the neutrinoless double beta decay rate on the mass of the extra states introduced to account for the Majorana masses of light neutrinos. For this purpose, we compute the nuclear matrix elements as functions of the mass of the mediating fermions and estimate the associated uncertainties. We then discuss what can be inferred on the seesaw model parameters in the different mass regimes and clarify how the contribution of the light neutrinos should always be taken into account when deriving bounds on the extra parameters. Conversely, the extra states can also have a significant impact, cancelling the Standard Model neutrino contribution for masses lighter than the nuclear scale and leading to vanishing neutrinoless double beta decay amplitudes even if neutrinos are Majorana particles. We also discuss how seesaw models could reconcile large rates of neutrinoless double beta decay with more stringent cosmological bounds on neutrino masses.
Mattias Blennow; Enrique Fernandez-Martinez; Jacobo Lopez-Pavon; Javier Menendez
Neutrinoless Double $?$-Decay: Status and Future
A brief summary of the status of neutrino masses, mixing and oscillations is presented. Neutrinoless double $\\beta$-decay is considered. Predictions for the effective Majorana mass are reviewed. A possible test of the calculations of nuclear matrix elements of the $0\
The Structure of a High Fidelity DNA Polymerase Bound to a Mismatched Nucleotide Reveals an ;Ajar; Intermediate Conformation in the Nucleotide Selection Mechanism
To achieve accurate DNA synthesis, DNA polymerases must rapidly sample and discriminate against incorrect nucleotides. Here we report the crystal structure of a high fidelity DNA polymerase I bound to DNA primer-template caught in the act of binding a mismatched (dG:dTTP) nucleoside triphosphate. The polymerase adopts a conformation in between the previously established 'open' and 'closed' states. In this 'ajar' conformation, the template base has moved into the insertion site but misaligns an incorrect nucleotide relative to the primer terminus. The displacement of a conserved active site tyrosine in the insertion site by the template base is accommodated by a distinctive kink in the polymerase O helix, resulting in a partially open ternary complex. We suggest that the ajar conformation allows the template to probe incoming nucleotides for complementarity before closure of the enzyme around the substrate. Based on solution fluorescence, kinetics, and crystallographic analyses of wild-type and mutant polymerases reported here, we present a three-state reaction pathway in which nucleotides either pass through this intermediate conformation to the closed conformation and catalysis or are misaligned within the intermediate, leading to destabilization of the closed conformation.
Wu, Eugene Y.; Beese, Lorena S. (Duke)
Progress Towards Doubling the Beam Power at Fermilab's Accelerator Complex
After a 14 month shutdown accelerator modifications and upgrades are in place to allow us doubling of the Main Injector beam power. We will discuss the past MI high power operation and the current progress towards doubling the power.
Kourbanis, ioanis
Sensitivity of CUORE to Neutrinoless Double-Beta Decay
Sensitivity of CUORE to Neutrinoless Double-Beta Decay b c,:Results of the Search for Neutrinoless D o u b l e - B e t ah e i n a , Evidence for neutrinoless double beta decay, M o
Alessandria, F.
Neutrinoless Double Beta Decay and Physics Beyond the Standard Model
Neutrinoless double beta decay is the most powerful tool to probe not only for Majorana neutrino masses but for lepton number violating physics in general. We discuss relations between lepton number violation, double beta decay and neutrino mass, review a general Lorentz invariant parametrization of the double beta decay rate, highlight a number of different new physics models showing how different mechanisms can trigger double beta decay, and finally discuss possibilities to discriminate and test these models and mechanisms in complementary experiments.
Frank F. Deppisch; Martin Hirsch; Heinrich P�s
/ Search for Neutrinoless Double Beta Decay with CUORE
neutrinoless double beta decay (??0?) in 130 Te and other rare processes. The observation of ??0? would
Elena Ferri
The Neutrinoless Double Beta Decay: The Case for Germanium Detectors
An overview of the current status of Neutrinoless Double Beta Decay is presented, emphasizing on the case of Germanium Detectors.
A. Morales; J. Morales
Geothermal: Sponsored by OSTI -- Structure of the Electric Double...
Office of Scientific and Technical Information (OSTI)
Structure of the Electric Double Layer in Hydrothermal Systems. Molecular Simulation Approach and Interpretation of Experimental Results...
Crystal structures of [lamda] exonuclease in complex with DNA suggest an electrostatic ratchet mechanism for processivity
The {lambda} exonuclease is an ATP-independent enzyme that binds to dsDNA ends and processively digests the 5'-ended strand to form 5' mononucleotides and a long 3' overhang. The crystal structure of {lambda} exonuclease revealed a toroidal homotrimer with a central funnel-shaped channel for tracking along the DNA, and a mechanism for processivity based on topological linkage of the trimer to the DNA was proposed. Here, we have determined the crystal structure of {lambda} exonuclease in complex with DNA at 1.88-{angstrom} resolution. The structure reveals that the enzyme unwinds the DNA prior to cleavage, such that two nucleotides of the 5'-ended strand insert into the active site of one subunit of the trimer, while the 3'-ended strand passes through the central channel to emerge out the back of the trimer. Unwinding of the DNA is facilitated by several apolar residues, including Leu78, that wedge into the base pairs at the single/double-strand junction to form favorable hydrophobic interactions. The terminal 5' phosphate of the DNA binds to a positively charged pocket buried at the end of the active site, while the scissile phosphate bridges two active site Mg{sup 2+} ions. Our data suggest a mechanism for processivity in which wedging of Leu78 and other apolar residues into the base pairs of the DNA restricts backward movement, whereas attraction of the 5' phosphate to the positively charged pocket drives forward movement of the enzyme along the DNA at each cycle of the reaction. Thus, processivity of {lambda} exonuclease operates not only at the level of the trimer, but also at the level of the monomer.
Zhang, Jinjin; McCabe, Kimberly A.; Bell, Charles E. (OSU)
The various processes of DNA uptake by cells can be categorized as: viral DNA entry, conjugation, or transformation. Within each category, a variety of mechanisms have been found. However, considerable similarities occur among the different mechanisms of conjugation and, especially, transformation. All of these natural mechanisms of DNA transfer are quite elaborate and involve multiple protein components, as the case may be, of the virus, the donor cell, and the recipient cell. The mechanisms of viral infection and conjugation will be discussed mainly with respect to their relevance to transformation.
Sequence independent amplification of DNA
The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example, the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei. 25 figs.
Bohlander, S.K.
The present invention is a rapid sequence-independent amplification procedure (SIA). Even minute amounts of DNA from various sources can be amplified independent of any sequence requirements of the DNA or any a priori knowledge of any sequence characteristics of the DNA to be amplified. This method allows, for example the sequence independent amplification of microdissected chromosomal material and the reliable construction of high quality fluorescent in situ hybridization (FISH) probes from YACs or from other sources. These probes can be used to localize YACs on metaphase chromosomes but also--with high efficiency--in interphase nuclei.
Bohlander, Stefan K. (Chicago, IL)
Normalized cDNA libraries
This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3' noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library.
Soares, Marcelo B. (New York, NY); Efstratiadis, Argiris (Englewood, NJ)
This invention provides a method to normalize a directional cDNA library constructed in a vector that allows propagation in single-stranded circle form comprising: (a) propagating the directional cDNA library in single-stranded circles; (b) generating fragments complementary to the 3{prime} noncoding sequence of the single-stranded circles in the library to produce partial duplexes; (c) purifying the partial duplexes; (d) melting and reassociating the purified partial duplexes to moderate Cot; and (e) purifying the unassociated single-stranded circles, thereby generating a normalized cDNA library. 4 figs.
Soares, M.B.; Efstratiadis, A.
Double Beta Decay Experiments Department of Physics and Astronomy
1 Double Beta Decay Experiments A. Piepkea a Department of Physics and Astronomy University. The experimen- tal investigation of the nuclear double beta decay is one of the key techniques for solving, such as the evaluation of the beta spec- tra near their endpoint, or neutrinoless double beta decay. The latter technique
Piepke, Andreas G.
of CUORE to Neutrinoless Double-Beta Decay b c,:L d e f F .s t results on neutrinoless double beta decay of T e w i t hthe study of neutrinoless double beta decay, J . C r y s t .
Homotopy BV-algebra structure on the double cobar construction
Homotopy BV-algebra structure on the double cobar construction Alexandre Quesney Abstract We show that the double cobar construction, 2C(X), of a simplicial set X is a homotopy BV-algebra if X is a double.2 The bar and cobar constructions . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 1.3 Hopf algebras
Paris-Sud XI, Universit� de
The Majorana Neutrinoless Double-Beta Decay Experiment
The Majorana Neutrinoless Double-Beta Decay Experiment Pre-conceptual Design Proposal November 22 Motivation for Neutrinoless Double-Beta Decay Experiments . . . . . . . . . 4 2.1.1 Community Guidance Neutrinoless Double-Beta Decay Results . . . . . . . . . . . . . . . . . . . . 12 2.5 Next
Washington at Seattle, University of - Department of Physics, Electroweak Interaction Research Group
Double K-Vacancy Production by X-Ray Photoionization
Double K-Vacancy Production by X-Ray Photoionization S. H. Southworth£ , R. W. Dunford£ , E. P Photon Source. Double K-vacancy production in Ne was observed by recording the KK-KLL Auger. For Mo, double K-vacancy production was observed by recording the K fluorescence hypersatellite
Importance of neutrinoless double beta decay
A natural explanation for the smallness of the neutrino mass requires them to be Majorana particles violating lepton number by two units. Since lepton number violation can have several interesting consequences in particle physics and cosmology, it is of utmost importance to find out if there is lepton number violation in nature and what is its magnitude. The neutrinoless double beta decay experiment can answer these questions: if there is lepton number violation and if neutrinos are Majorana particles. In addition, the magnitude of neutrinoless double beta decay will constrain any other lepton number violating processes. This lepton number violation may also be relatd to the matter-antimatter asymmetry of the universe, dark matter and cosmological constant.
Utpal Sarkar
Crystal Structure of the Human NKX2.5 Homeodomain in Complex with DNA Target
NKX2.5 is a homeodomain containing transcription factor regulating cardiac formation and function, and its mutations are linked to congenital heart disease. Here we provide the first report of the crystal structure of the NKX2.5 homeodomain in complex with double-stranded DNA of its endogenous target, locating within the proximal promoter -242 site of the atrial natriuretic factor gene. The crystal structure, determined at 1.8 {angstrom} resolution, demonstrates that NKX2.5 homeodomains occupy both DNA binding sites separated by five nucleotides without physical interaction between themselves. The two homeodomains show identical conformation despite the differences in the DNA sequences they bind, and no significant bending of the DNA was observed. Tyr54, absolutely conserved in NK2 family proteins, mediates sequence-specific interaction with the TAAG motif. This high resolution crystal structure of NKX2.5 protein provides a detailed picture of protein and DNA interactions, which allows us to predict DNA binding of mutants identified in human patients.
Pradhan, Lagnajeet; Genis, Caroli; Scone, Peyton; Weinberg, Ellen O.; Kasahara, Hideko; Nam, Hyun-Joo (BU-M); (Florida); (Texas)
Neutrinoless Double Beta Decay and its "Inverse"
Recent considerations by these authors pointed out the attractive features which a search for the exchange of heavy Majorana neutrinos could have for solving the mass and the lepton number puzzles for all neutrinos, in TeV-level electron-electron scattering. In the present note, we show that, contrary to subsequently published arguments, non-observation of neutrinoless double beta decay has, to date, no bearing on the promise of this important task for future linear electron colliders.
Neutrinoless Double Beta Decay in Particle Physics
Neutrinoless double beta decay is a process of fundamental importance for particle physics. It can be mediated by light massive Majorana neutrinos (standard interpretation) or by something else (non-standard interpretations). We review its dependence on the neutrino parameters, its complementarity to other observables sensitive to neutrino mass, and emphasize its ability to distinguish different neutrino mass models. Then we discuss mechanisms different from light Majorana neutrino exchange, and show what can be learned from those and how they could be tested.
Double-clad nuclear fuel safety rod
A device for shutting down a nuclear reactor during an undercooling or overpower event, whether or not the reactor's scram system operates properly. This is accomplished by double-clad fuel safety rods positioned at various locations throughout the reactor core, wherein melting of a secondary internal cladding of the rod allows the fuel column therein to shift from the reactor core to place the reactor in a subcritical condition.
McCarthy, William H. (Los Altos, CA); Atcheson, Donald B. (Cupertino, CA); Vaidyanathan, Swaminathan (San Jose, CA)
Double beta decays of {sup 106}Cd
The two-neutrino (2{nu}2{beta}) and neutrinoless (0{nu}2{beta}) double beta decays of {sup 106}Cd are studied for the transitions to the ground state 0{sub gs}{sup +} and 0{sup +} and 2{sup +} excited states in {sup 106}Pd by using realistic many-body wave functions calculated in the framework of the quasiparticle random-phase approximation. Effective, G-matrix-derived nuclear forces are used in realistic single-particle model spaces. All the possible channels, {beta}{sup +}{beta}{sup +}, {beta}{sup +}EC, and ECEC, are discussed for both the 2{nu}2{beta} and 0{nu}2{beta} decays. The associated half-lives are computed and particular attention is devoted to the study of the detectability of the resonant neutrinoless double electron capture (R0{nu}ECEC) process in {sup 106}Cd. The calculations of the present article constitute the thus far most complete and up-to-date investigation of the double-beta-decay properties of {sup 106}Cd.
Suhonen, Jouni [Department of Physics, P.O. Box 35 (YFL), FI-40014 University of Jyvaeskylae (Finland)
Double-Difference Tomography for Sequestration MVA
Analysis of synthetic data was performed to determine the most cost-effective tomographic monitoring system for a geologic carbon sequestration injection site. Double-difference tomographic inversion was performed on 125 synthetic data sets: five stages of CO2 plume growth, five seismic event regions, and five geophone arrays. Each resulting velocity model was compared quantitatively to its respective synthetic velocity model to determine an accuracy value. The results were examined to determine a relationship between cost and accuracy in monitoring, verification, and accounting applications using double-difference tomography. The geophone arrays with widely-varying geophone locations, both laterally and vertically, performed best. Additionally, double difference seismic tomography was performed using travel time data from a carbon sequestration site at the Aneth oil field in southeast Utah as part of a Department of Energy initiative on monitoring, verification, and accounting (MVA) of sequestered CO2. A total of 1,211 seismic events were recorded from a borehole array consisting of 22 geophones. Artificial velocity models were created to determine the ease with which different CO2 plume locations and sizes can be detected. Most likely because of the poor geophone arrangement, a low velocity zone in the Desert Creek reservoir can only be detected when regions of test site containing the highest ray path coverage are considered. MVA accuracy and precision may be improved through the use of a receiver array that provides more comprehensive ray path coverage.
Westman, Erik
A background free double beta decay experiment
We present a new detection scheme for rejecting backgrounds in neutrino less double beta decay experiments. It relies on the detection of Cherenkov light emitted by electrons in the MeV region. The momentum threshold is tuned to reach a good discrimination between background and good events. We consider many detector concepts and a range of target materials. The most promising is a high-pressure 136Xe emitter for which the required energy threshold is easily adjusted. Combination of this concept and a high pressure Time Projection Chamber could provide an optimal solution. A simple and low cost effective solution is to use the Spherical Proportional Counter that provides two delayed signals from ionization and Cherenkov light. In solid-state double beta decay emitters, because of their higher density, the considered process is out of energy range. An alternative solution could be the development of double decay emitters with lower density by using for instance the aerogel technique. It is surprising that a technology used for particle identification in high-energy physics becomes a powerful tool for rejecting backgrounds in such low-energy experiments.
Ioannis Giomataris
We present a new detection scheme for rejecting backgrounds in neutrino less double beta decay experiments. It relies on the detection of Cherenkov light emitted by electrons in the MeV region. The momentum threshold is tuned to reach a good discrimination between background and good events. We consider many detector concepts and a range of target materials. The most promising is a high-pressure 136Xe emitter for which the required energy threshold is easily adjusted. Combination of this concept and a high pressure Time Projection Chamber could provide an optimal solution. A simple and low cost effective solution is to use the Spherical Proportional Counter that provides two delayed signals from ionization and Cherenkov light. In solid-state double beta decay emitters, because of their higher density, the considered process is out of energy range. An alternative solution could be the development of double decay emitters with lower density by using for instance the aerogel technique. It is surprising that a te...
Giomataris, Ioannis
Modeling DNA Shuffling Fengzhu Sun
Modeling DNA Shuffling Fengzhu Sun 1Department of Genetics Emory University School of Medicine property are selected. Irvine et al. (1991) and Sun et al. (1996) studied in vitro evolution not involving
Sun, Fengzhu - Sun, Fengzhu
Unnatural nucleotides for DNA sequencing
Fluorescent nucleotide analogs were prepared and tested to find surrogate structures that are: (i) incorporated by DNA polymerases; (ii) spectroscopically distinct for each fluorescent tag; and (iii) easily deprotected at the 3'-position under mild...
Jacutin, Swanee E
Molecular Computing with DNA Self-Assembly
Molecular Computing with DNA Self-Assembly Urmi Majumder #12;Self-Assembly in Nature #12;Key to DNA for Molecular Computing with DNA Self-Assembly Compact: Small library of assembly primitives Complex: Capable in Tiling Assembly: vitroation tural DNA self-assembly has powerful echanisms for error correction
Exploring the Neutrinoless Double Beta Decay in the Inverted Neutrino Hierarchy with Bolometric Detectors
Exploring the Neutrinoless Double Beta Decay in the InvertedC. Giunti, Neutrinoless double-beta decay. A brief review,el- ements for neutrinoless double-beta decay and double-
Artusa, D. R.
CONSULTATION RESPONSE The Forensic Use of DNA and the National DNA Database
CONSULTATION RESPONSE The Forensic Use of DNA and the National DNA Database Wellcome Trust response on the important topic of the forensic use of DNA and the National DNA Database (NDNAD). Given the Trust Assembly; "Forensic DNA Databasing: A European perspective" - a biomedical ethics grant to Professor Robin
Rambaut, Andrew
DNA polymorphism identity determination using flow cytometry
DNA polymorphism identity determination using flow cytometry. Primers designed to be immobilized on microspheres are allowed to anneal to the DNA strand under investigation, and are extended by either DNA polymerase using fluorescent dideoxynucleotides or ligated by DNA ligase to fluorescent reporter oligonucleotides. The fluorescence of either the dideoxynucleotide or the reporter oligonucleotide attached to the immobilized primer is measured by flow cytometry, thereby identifying the nucleotide polymorphism on the DNA strand.
Nolan, John P. (Santa Fe, NM); White, P. Scott (Los Alamos, NM); Cai, Hong (Los Alamos, NM)
Cellular responses to environmental DNA damage
This volume contains the proceedings of the conference entitled Cellular Responses to Environmental DNA Damage held in Banff,Alberta December 1--6, 1991. The conference addresses various aspects of DNA repair in sessions titled DNA repair; Basic Mechanisms; Lesions; Systems; Inducible Responses; Mutagenesis; Human Population Response Heterogeneity; Intragenomic DNA Repair Heterogeneity; DNA Repair Gene Cloning; Aging; Human Genetic Disease; and Carcinogenesis. Individual papers are represented as abstracts of about one page in length.
Not Available
Nuclear matrix elements for neutrinoless double-beta decay and double-electron capture
A new generation of neutrinoless double beta decay experiments with improved sensitivity is currently under design and construction. They will probe inverted hierarchy region of the neutrino mass pattern. There is also a revived interest to the resonant neutrinoless double-electron capture, which has also a potential to probe lepton number conservation and to investigate the neutrino nature and mass scale. The primary concern are the nuclear matrix elements. Clearly, the accuracy of the determination of the effective Majorana neutrino mass from the measured 0\
Amand Faessler; Vadim Rodin; Fedor Simkovic
Regulation of DNA damage tolerance : studies of the translesion synthesis DNA ploymerase eta in Saccharomyces cerevisiae
All organisms must control the effects of DNA damage to protect the integrity of their genomes. In addition to DNA repair, this requires DNA damage tolerance pathways, which allow the continuation of essential processes ...
Woodruff, Rachel Van Etten
Neutrinoless double beta decay and QCD corrections
We consider one loop QCD corrections and renormalization group running of the neutrinoless double beta decay amplitude focusing on the short-range part of the amplitude (without the light neutrino exchange) and find that these corrections can be sizeable. Depending on the operator under consideration, there can be moderate to large cancellations or significant enhancements. We discuss several specific examples in this context. Such large corrections will lead to significant shifts in the half-life estimates which currently are known to be plagued with the uncertainties due to nuclear physics inputs to the physical matrix elements.
Namit Mahajan
Simulated progress in double-beta decay
A Monte Carlo code has been developed to accurately simulate double-beta decay measurements. Coincident gamma rays, beta spectra, and angular correlations have been added to adequately simulate a complete {sup 100}Mo nuclear decay and provide corrections to experimentally determined detector efficiencies. This code has been used to strip certain low-background spectra obtained in the Homestake gold mine in Lead, SD, for the purpose of extremely sensitive materials assay for the construction of new, large, enriched germanium detectors. Assays as low as 9 {mu}Bq/g of {sup 210}Pb in lead shielding were obtained.
Miley, H.S.; Arthur, R.J. [Pacific Northwest Lab., Richland, WA (United States); Avignone, F.T. [South Carolina Univ., Columbia, SC (United States)] [and others
Double acting stirling engine phase control
A mechanical device for effecting a phase change between the expansion and compression volumes of a double-acting Stirling engine uses helical elements which produce opposite rotation of a pair of crankpins when a control rod is moved, so the phase between two pairs of pistons is changed by +.psi. and the phase between the other two pairs of pistons is changed by -.psi.. The phase can change beyond .psi.=90.degree. at which regenerative braking and then reversal of engine rotation occurs.
Berchowitz, David M. (Scotia, NY)
Neutrinoless double beta decay and neutrino masses
Neutrinoless double beta decay (0{nu}{beta}{beta}) is a promising test for lepton number violating physics beyond the standard model (SM) of particle physics. There is a deep connection between this decay and the phenomenon of neutrino masses. In particular, we will discuss the relation between 0{nu}{beta}{beta} and Majorana neutrino masses provided by the so-called Schechter-Valle theorem in a quantitative way. Furthermore, we will present an experimental cross check to discriminate 0{nu}{beta}{beta} from unknown nuclear background using only one isotope, i.e., within one experiment.
Duerr, Michael [Max-Planck-Institut fuer Kernphysik, Saupfercheckweg 1, 69117 Heidelberg (Germany)
Complete characterization of double photoionization processes
We analyze correlated photoelectron spectra of single-photon two-electron ionization [double photoionization (DPI)] of helium to reconstruct the phase of the spectral amplitude of this process. The phase can be reconstructed reliably in a wide range of photoelectron momenta, thus allowing one to retrieve information about the wave function of the DPI process and its temporal evolution. Our simulation indicates that the proposed phase reconstruction technique can be applied in experiment to trace dynamics of the DPI process with attosecond precision.
Ivanov, I. A.; Kheifets, A. S. [Research School of Physical Sciences, Australian National University, Canberra ACT 0200 (Australia)
Structure and Mutagenesis of the Parainfluenza Virus 5 Hemagglutinin-Neuraminidase Stalk Domain Reveals a Four-Helix Bundle and the Role of the Stalk in Fusion Promotion
Paramyxovirus entry into cells requires the fusion protein (F) and a receptor binding protein (hemagglutinin-neuraminidase [HN], H, or G). The multifunctional HN protein of some paramyxoviruses, besides functioning as the receptor (sialic acid) binding protein (hemagglutinin activity) and the receptor-destroying protein (neuraminidase activity), enhances F activity, presumably by lowering the activation energy required for F to mediate fusion of viral and cellular membranes. Before or upon receptor binding by the HN globular head, F is believed to interact with the HN stalk. Unfortunately, until recently none of the receptor binding protein crystal structures have shown electron density for the stalk domain. Parainfluenza virus 5 (PIV5) HN exists as a noncovalent dimer-of-dimers on the surface of cells, linked by a single disulfide bond in the stalk. Here we present the crystal structure of the PIV5-HN stalk domain at a resolution of 2.65 {angstrom}, revealing a four-helix bundle (4HB) with an upper (N-terminal) straight region and a lower (C-terminal) supercoiled part. The hydrophobic core residues are a mix of an 11-mer repeat and a 3- to 4-heptad repeat. To functionally characterize the role of the HN stalk in F interactions and fusion, we designed mutants along the PIV5-HN stalk that are N-glycosylated to physically disrupt F-HN interactions. By extensive study of receptor binding, neuraminidase activity, oligomerization, and fusion-promoting functions of the mutant proteins, we found a correlation between the position of the N-glycosylation mutants on the stalk structure and their neuraminidase activities as well as their abilities to promote fusion.
Bose, Sayantan; Welch, Brett D.; Kors, Christopher A.; Yuan, Ping; Jardetzky, Theodore S.; Lamb, Robert A. (NWU); (Stanford-MED)
Search For Neutrinoless Double Beta Decay
The HEIDELBERG-MOSCOW experiment, which is the most sensitive double beta decay experiment since ten years has been regularly continued until end of November 2003. An analysis of the data has been performed already until May 20, 2003. The experiment yields now, on a 4? level, evidence for lepton number violation and proves that the neutrino is a Majorana particle. It further shows that neutrino masses are degenerate. In addition it puts several stringent constraints on other physics beyond the Standard Model. Among others it opens the door to test various supersymmetric theory scenarios, for example it gives the sharpest in the R-parity violating part of the superpotential, and limit on the parameter ? ? 111 gives information on the splitting of the sneutrino-antisneutrino system. The result from the HEIDELBERG-MOSCOW experiment is consistent with recent results from CMB investigations, with high energy cosmic rays, with the result from the g-2 experiment and with recent theoretical work. It is indirectly supported by the analysis of other Ge double beta experiments. Recent criticism of various kind has been shown to be wrong, among others by measurements performed in 2003 with a 214Bi source ( 226Ra), by simulation of the background in the range of Q?? by GEANT4, and by deeper investigation of statistical features such as sensitivity of peak search, and relevance of width of window of analysis. 1
H. V. Klapdor-kleingrothaus *a; I. V. Krivosheina A; A. Dietz A
Neutrinoless Double Beta Decay in Supersymmetric Seesaw model
Inspired by the recent HEIDELBERG-MOSCOW double beta decay experiment, we discuss the neutrinoless double beta decay in the supersymmetric seesaw model. Our numerical analysis indicates that we can naturally explain the data of the observed neutrinoless double beta decay, as well as that of the solar and atmospheric neutrino experiments with at least one Majorana-like sneutrino of middle energy scale in the model.
Tai-Fu Feng; Xue-Qian Li; Yan-An Luo
Search for neutrinoless double beta decay with NEMO 3 experiment
NEMO 3 experiment is designed to search for neutrinoless double beta decay. It is located in the Modane Underground Laboratory (LSM) and has been taking data since February 2003. The half- lives of two neutrino beta decay have been measured for seven isotopes. No evidence of neutrinoless double beta decay has been found. The limits on both the half-lives of the neutrinoless double beta decay and the corresponding Majorana effective masses are derived
Zornitza Daraktchieva
Neutrinoless double beta decay search with the NEMO 3 experiment
The NEMO 3 experiment searches for neutrinoless double beta decay and makes precision measurements of two-neutrino double beta decay in seven isotopes. The latest two-neutrino half-life results are presented, together with the limits on neutrinoless half-lives and the corresponding effective Majorana neutrino masses. Also given are the limits obtained on neutrinoless double beta decay mediated by Rp-violating SUSY, right-hand currents and different Majoron emission modes.
Irina Nasteva; for the NEMO 3 Collaboration
The NEMO 3 experiment searches for neutrinoless double beta decay and makes precision measurements of two-neutrino double beta decay in seven isotopes. The latest two-neutrino half-life results are presented, together with the limits on neutrinoless half-lives and the corresponding effective Majorana neutrino masses. Also given are the limits obtained on neutrinoless double beta decay mediated by R{sub p}-violating SUSY, right-hand currents and different Majoron emission modes.
Nasteva, Irina [Particle Physics Group, School of Physics and Astronomy, University of Manchester, Manchester, M13 9PL (United Kingdom)
Results of the NEMO-3 Double Beta Decay Experiment
The NEMO-3 experiment is searching for neutrinoless double beta decay for 2 main isotopes (100Mo and 82Se) and is studying the two-neutrino double beta decay of seven isotopes. The experiment has been taking data since 2003 and, up to the end of 2009, showed no evidence for neutrinoless double beta decay. Two 90 % CL lower limits on the half-lives of the transitions were obtained : T_{1/2}^{0\
Mathieu BONGRAND for the NEMO-3 Collaboration
Surface-plasmons lasing in double-graphene-layer structures
We consider the concept of injection terahertz lasers based on double-graphene-layer (double-GL) structures with metal surface-plasmon waveguide and study the conditions of their operation. The laser under consideration exploits the resonant radiative transitions between GLs. This enables the double-GL laser room temperature operation and the possibility of voltage tuning of the emission spectrum. We compare the characteristics of the double-GL lasers with the metal surface-plasmon waveguides with those of such laser with the metal-metal waveguides.
Dubinov, A. A. [Research Institute for Electrical Communication, Tohoku University, Sendai 980-8577 (Japan); Institute for Physics of Microstructures of Russian Academy of Sciences, and Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603950 (Russian Federation); Aleshkin, V. Ya. [Institute for Physics of Microstructures of Russian Academy of Sciences, and Lobachevsky State University of Nizhny Novgorod, Nizhny Novgorod 603950 (Russian Federation); Ryzhii, V. [Research Institute for Electrical Communication, Tohoku University, Sendai 980-8577 (Japan); Center for Photonics and Infrared Engineering, Bauman Moscow State Technical University, Moscow 105005 (Russian Federation); Shur, M. S. [Department of Electrical, Electronics, and System Engineering, Rensselaer Polytechnic Institute, Troy, New York 12180 (United States); Otsuji, T. [Research Institute for Electrical Communication, Tohoku University, Sendai 980-8577 (Japan)
Half-lives of Double $?^+$-decay with Two Neutrinos
Nuclear double $\\beta ^-$-decays with two neutrinos were observed for many years and a systematic law describing the relation between their half-lives and decay energies was also proposed recently [Phys. Rev. C89, 064603 (2014)]. However, double $\\beta ^+$-decay ($\\beta ^+\\beta^+)$ with emission of both two positrons and two neutrinos has not been observed up to date. In this article, we perform a systematic analysis on the candidates of double $\\beta ^+$-decay, based on the 2012 nuclear mass table. Eight nuclei are found to be the good candidates for double $\\beta ^+$-decay and their half-lives are predicted according to the generalization of the systematic law to double $\\beta ^+$-decay. As far as we know, there is no theoretical result on double $\\beta ^+$-decay of nucleus $^{154}Dy$ and our result is the first prediction on this nucleus. This is also the first complete research on eight double $\\beta ^+$-decay candidates based on the available data of nuclear masses. It is expected that the calculated half-lives of double $\\beta ^+$-decay in this article will be useful for future experimental search of double $\\beta ^+$-decay.
Yuejiao Ren; Zhongzhou Ren
Electromagnetically Induced Transparency in a Double Well Atomic Josephson Junction
observation of these Josephson junction resonances. 2.dressed Bose condensed Josephson junction Let us consider ain a Double Well Atomic Josephson Junction J.O. Weatherall
Weatherall, J. O.; Search, C. P.
Neutrinoless double beta decay can constrain neutrino dark matter
We examine how constraints can be placed on the neutrino component of dark matter by an accurate measurement of neutrinoless double beta ($0\
V. Barger; S. L. Glashow; D. Marfatia; K. Whisnant
Majorana Neutrino Masses from Neutrinoless Double Beta Decay and Cosmology
When three Majorana neutrinos describe the solar and atmospheric neutrino data via oscillations, a nonzero measurement of neutrinoless double beta ($0\
V. Barger; K. Whisnant
A Search for Neutrinoless Double Beta Decay of Te-130.
??This dissertation describes an experimental search for neutrinoless double beta (0???) decay of 130Te. An observation of 0??? decay would establish that neutrinos are Majorana� (more)
2005 Double white lines on bills of Razorbill 103 DOUBLE WHITE LINES ON THE BILL OF THE
2005 Double white lines on bills of Razorbill 103 DOUBLE WHITE LINES ON THE BILL OF THE RAZORBILL.L., MUZAFFAR S.B. & JONES I.L. Lavers J.L., Muzaffar S.B. & Jones I.L. 2006. Double white lines on the bill Seabirds 7(3): 103-108. One of the diagnostic features of the Razorbill Alca torda is the distinct white
Jones, Ian L.
DNA Concentration By UV Spectrophotometry Measure Absorption
DNA Concentration By UV Spectrophotometry Measure Absorption: 1. Dilute DNA to 0.5 to 50 µg 2. Measure absorption at 260 nm (A260). Start by zeroing instrument with TE buffer or dH2O alone
Aris, John P.
Micropatterned cell arrays for detecting DNA damage
Numerous agents are capable of interacting with DNA and damaging it. Permanent changes in the DNA structure can be both mutagenic and cytotoxic; therefore, methods to measure the susceptibility of cells to mutations are ...
Mittal, Sukant
Towards Privacy Preserving of Forensic DNA Databases
Protecting privacy of individuals is critical for forensic genetics. In a kinship/identity testing, related DNA profiles between user's query and the DNA database need to be extracted. However, unrelated profiles cannot be revealed to each other...
Liu, Sanmin
Chromosome specific repetitive DNA sequences
A method is provided for determining specific nucleotide sequences useful in forming a probe which can identify specific chromosomes, preferably through in situ hybridization within the cell itself. In one embodiment, chromosome preferential nucleotide sequences are first determined from a library of recombinant DNA clones having families of repetitive sequences. Library clones are identified with a low homology with a sequence of repetitive DNA families to which the first clones respectively belong and variant sequences are then identified by selecting clones having a pattern of hybridization with genomic DNA dissimilar to the hybridization pattern shown by the respective families. In another embodiment, variant sequences are selected from a sequence of a known repetitive DNA family. The selected variant sequence is classified as chromosome specific, chromosome preferential, or chromosome nonspecific. Sequences which are classified as chromosome preferential are further sequenced and regions are identified having a low homology with other regions of the chromosome preferential sequence or with known sequences of other family me This invention is the result of a contract with the Department of Energy (Contract No. W-7405-ENG-36).
Moyzis, Robert K. (Los Alamos, NM); Meyne, Julianne (Los Alamos, NM)
International Standards in Forensic DNA
International Standards in Forensic DNA John M. Butler, Ph.D. National Institute of Standards and Technology NIST Fellow & Special Assistant to the Director for Forensic Science Vice-Chair, National Commission on Forensic Science World Forensics Festival Seoul, Korea October 15, 2014 #12;Definition
Double beta decay and neutrino mass models
Neutrinoless double beta decay allows to constrain lepton number violating extensions of the standard model. If neutrinos are Majorana particles, the mass mechanism will always contribute to the decay rate, however, it is not a priori guaranteed to be the dominant contribution in all models. Here, we discuss whether the mass mechanism dominates or not from the theory point of view. We classify all possible (scalar-mediated) short-range contributions to the decay rate according to the loop level, at which the corresponding models will generate Majorana neutrino masses, and discuss the expected relative size of the different contributions to the decay rate in each class. We also work out the phenomenology of one concrete 2-loop model in which both, mass mechanism and short-range diagram, might lead to competitive contributions, in some detail.
Helo, J C; Ota, T; Santos, F A Pereira dos
Neutrinoless Double Beta Decay with SNO+
SNO+ will search for neutrinoless double beta decay by loading 780 tonnes of linear alkylbenzene liquid scintillator with O(tonne) of neodymium. Using natural Nd at 0.1% loading will provide 43.7 kg of 150Nd given its 5.6% abundance and allow the experiment to reach a sensitivity to the effective neutrino mass of 100-200 meV at 90% C.L in a 3 year run. The SNO+ detector has ultra low backgrounds with 7000 tonnes of water shielding and self-shielding of the scintillator. Distillation and several other purification techniques will be used with the aim of achieving Borexino levels of backgrounds. The experiment is fully funded and data taking with light-water will commence in 2012 with scintillator data following in 2013.
J. Hartnell; for the SNO+ collaboration
Correlations and the neutrinoless double beta decay
We explore the influence of the deformation on the nuclear matrix elements of the neutrinoless double beta decay (NME), concluding that the difference in deformation -or more generally on the amount of quadrupole correlations- between parent and grand daughter nuclei quenchs strongly the decay. We discuss how varies the nuclear matrix element of {sup 76}Ge decay when the wave functions of the two nuclei involved in the transition are constrained to reproduce the experimental occupancies. In the Interacting Shell Model description the value of the NME is enhanced about 15% compared to previous calculations, whereas in the QRPA the NME's are reduced by 20%-30%, thus, the discrepancies between both approaches diminish.
Menendez, J.; Poves, A. [Departamento de Fisica Teorica, and IFT, UAM-CSIC, Universidad Autonoma de Madrid, 28049-Madrid (Spain); Caurier, E.; Nowacki, F. [IPHC, IN2P3-CNRS/Universite Louis Pasteur, 67037-Strasbourg (France)
Experimental searches of neutrinoless double beta decay
Neutrinoless double decay is a unique probe for lepton number conservation and neutrino properties. It allows to investigate the Dirac/Majorana nature of the neutrinos and their absolute mass scale (hierarchy problem) with unprecedented sensitivity. A number of experiments are presently under preparation to cover the quasi-degenerate region of the neutrino mass spectrum. Improved sensitivities are however required to sound the so-called inverted hierarchy region. This is a real challenge faced by a number of new proposed projects, based either on large expansions of the present experiments or on new ideas top improve the technical performance or reduce the background contributions. A review of the most relevant ongoing experiments is given. The most relevant parameters contributing to the experimental sensitivity are discussed and a critical comparison of the future projects is proposed.
Double Shell Tank (DST) Utilities Specification
This specification establishes the performance requirements and provides the references to the requisite codes and standards to he applied during the design of the Double-Shell Tank (DST) Utilities Subsystems that support the first phase of waste feed delivery (WFD). The DST Utilities Subsystems provide electrical power, raw/potable water, and service/instrument air to the equipment and structures used to transfer low-activity waste (LAW) and high-level waste (HLW) to designated DST staging tanks. The DST Utilities Subsystems also support the equipment and structures used to deliver blended LAW and HLW feed from these staging tanks to the River Protection Project (RPP) Privatization Contractor facility where the waste will be immobilized. This specification is intended to be the basis for new projects/installations. This specification is not intended to retroactively affect previously established project design criteria without specific direction by the program.
The Double Copy Structure of Soft Gravitons
The subleading corrections to factorization theorems for soft bremsstrahlung in nonabelian gauge theories and gravity are investigated. Building on recent results, we write the action of the angular momentum operators on scattering amplitudes as derivatives with respect to the Mandelstam invariants to uncover a double copy structure in the contribution of the soft graviton to the amplitude, both in the leading term and the first correction. Using our approach, we study Gribov's theorem as extended to nonabelian gauge theories and gravity by Lipatov, and find that subleading corrections can be obtained from those to Low's theorem by dropping the terms with derivatives with respect to the center-of-mass energy, which are suppressed at high energies. In this case, the emitted gravitons are not necessarily soft.
Agustin Sabio Vera; Miguel A. Vazquez-Mozo
A hybrid double-dot in silicon
We report electrical measurements of a single arsenic dopant atom in the tunnel barrier of a silicon single-electron transistor (SET). In addition to performing the electrical characterisation of the individual dopants, we study the series electrical transport through the dopant and SET. We measure the bias triangles of this hybrid double-dot and show that we can tune the electrostatic coupling between the two sub-systems. Additionally, we measured SET in which an additional plunger gate allows the reduction of the electron number in the SET down to the few-electron regime where the dot presents well-defined spin configurations. Finally, we discuss the challenges of operating a dopant-dot hybrid system in the few-electron regime.
Gonzalez-Zalba, M. F.; Heiss, D.; Ferguson, A. J. [Cavendish Laboratory, University of Cambridge, JJ Thomson Avenue, Cambridge (United Kingdom)
Neutrinoless Double Beta Decay in Gauge Theories
Neutrinoless double beta decay is a very important process both from the particle and nuclear physics point of view. Its observation will severely constrain the existing models and signal that the neutrinos are massive Majorana particles. From the elementary particle point of view it pops up in almost every model. In addition to the traditional mechanisms, like the neutrino mass, the admixture of right handed currents etc, it may occur due to the R-parity violating supersymmetric (SUSY) interactions. From the nuclear physics point of view it is challenging, because: 1) The relevant nuclei have complicated nuclear structure. 2) The energetically allowed transitions are exhaust a small part of all the strength. 3) One must cope with the short distance behavior of the transition operators, especially when the intermediate particles are heavy (eg in SUSY models). Thus novel effects, like the double beta decay of pions in flight between nucleons, have to be considered. 4) The intermediate momenta involved are about 100 MeV. Thus one has to take into account possible momentum dependent terms in the nucleon current. We find that, for the mass mechanism, such modifications of the nucleon current for light neutrinos reduce the nuclear matrix elements by about 25 per cent, almost regardless of the nuclear model. In the case of heavy neutrinos the effect is much larger and model dependent. Taking the above effects into account, the available nuclear matrix elements for the experimentally interesting nuclei A = 76, 82, 96, 100, 116, 128, 130, 136 and 150 and the experimental limits on the life times we have extracted new stringent limits on the average neutrino mass and on the R-parity violating coupling for various SUSY models.
J. D. Vergados
Amplification of chromosomal DNA in situ
Amplification of chromosomal DNA in situ to increase the amount of DNA associated with a chromosome or chromosome region is described. The amplification of chromosomal DNA in situ provides for the synthesis of Fluorescence in situ Hybridization (FISH) painting probes from single dissected chromosome fragments, the production of cDNA libraries from low copy mRNAs and improved in Comparative Genomic Hybridization (CGH) procedures.
Christian, Allen T. (Tracy, CA); Coleman, Matthew A. (Livermore, CA); Tucker, James D. (Livermore, CA)
DNA nanotubes and helical nanotapes via self-assembly of ssDNA-amphiphiles
DNA nanotubes and helical nanotapes via self- assembly of ssDNA-amphiphiles Timothy R. Pearcea and Efrosini Kokkoli*b DNA nanotubes were created using molecular self-assembly of single-stranded DNA (ss. The nanotube structures were formed by bilayers of amphiphiles, with the hydrophobic components forming
Kokkoli, Efie
Supplementary Information for: Integrating DNA Strand Displacement Circuitry with DNA Tile Self-assembly
Supplementary Information for: Integrating DNA Strand Displacement Circuitry with DNA Tile Self-assembly of Contents: · Supplementary Figures 1. Native polyacrylamide gel electrophoresis of DNA tile self-assembly 2. UV absorbance annealing and melting curves of DNA tile self-assembly 3. Characterization
Zhang, David Yu
Single-Molecule Study of DNA Polymerization Activity of HIV-1 Reverse Transcriptase on DNA Templates
Single-Molecule Study of DNA Polymerization Activity of HIV-1 Reverse Transcriptase on DNA unwinding protein, such as a helicase. In order to understand the mechanism of polymerization through secondary structures, we investigated the DNA polymerization activity of HIV-1 RT on long ssDNA templates
Xie, Xiaoliang Sunney
Probe and method for DNA detection
A hybridization probe containing two linear strands of DNA lights up upon hybridization to a target DNA using silver nanoclusters that have been templated onto one of the DNA strands. Hybridization induces proximity between the nanoclusters on one strand and an overhang on the other strand, which results in enhanced fluorescence emission from the nanoclusters.
Yeh, Hsin-Chih; Werner, James Henry; Sharma, Jaswinder Kumar; Martinez, Jennifer Suzanne
Self-assembled DNA Structures for Nanoconstruction
Self-assembled DNA Structures for Nanoconstruction Hao Yan, Peng Yin, Sung Ha Park, Hanying Li methods based on DNA self-assembly. Here we review our recent experimental progress to utilize novel DNA nanostructures for self-assembly as well as for templates in the fabrication of functional nano
Yin, Peng
HELICASE DEPENDENT AMPLICATION transfer the DNA
STEP 1 STEP 2 STEP 3 HELICASE DEPENDENT AMPLICATION HELICASE DNA POLYMERASE transfer the DNA instrumentation. MOLECULAR BIOMARKERS MADE UP DNA or RNA STRANDS ARE A POWERFUL WEAPON IN DETECTING DISEASE. USING for sample preparation at the point to care using only hand generated power.... #12;
Significant neutrinoless double beta decay with quasi-Dirac neutrinos
A significant signal of neutrinoless double beta decay can be consistent with the existence of light quasi-Dirac neutrinos. To demonstrate this possibility, we consider a realistic model where the neutrino masses and the neutrinoless double beta decay can be simultaneously generated after a Peccei-Quinn symmetry breaking.
Pei-Hong Gu
One Year of Evidence for Neutrinoless Double Beta Decay
The present experimental status in the search for neutrinoless double beta decay is reviewed, with emphasis on the first indication for neutrinoless double beta decay found in the HEIDELBERG-MOSCOW experiment, giving first evidence for lepton number violation and a Majorana nature of the neutrinos. Future perspectives of the field are briefly outlined.
H. V. Klapdor-Kleingrothaus
Support of Evidence for Neutrinoless Double Beta Decay
Indirect support for the evidence of neutrinoless double beta decay reported recently, is obtained by analysis of other Ge double beta experiments, which yield independent information on the background in the region of Q_(beta-beta). Some statistical features as well as background simulations with GEANT 4 of the HEIDELBERG-MOSCOW experiment are discussed which disprove recent criticism.
H. V. Klapdor-Kleingrothaus; A. Dietz; I. V. Krivosheina; Ch. Doerr; C. Tomei
Deep subwavelength plasmonic waveguide switch in double graphene layer structure
Deep subwavelength plasmonic waveguide switch in double graphene layer structure Hideo Iizuka in double graphene layer structure Hideo Iizuka1,a) and Shanhui Fan2,b) 1 Toyota Central Research December 2013) Graphene provides excellent prospects of the dynamic tunability, low propagation loss
Fan, Shanhui
Strategies for Next Generation Neutrinoless Double-Beta Decay Experiments
Strategies for Next Generation Neutrinoless Double-Beta Decay Experiments F. T. Avignone III A brief discussion of the connection between neutrino oscillation data and predictions of neutrinoless the necessary tools for comparative evaluation. 1. INTRODUCTION Neutrinoless double-beta (0)-decay has been
Neutrinoless double-beta decay. A brief review
In this brief review we discuss the generation of Majorana neutrino masses through the see-saw mechanism, the theory of neutrinoless double-beta decay, the implications of neutrino oscillation data for the effective Majorana mass, taking into account the recent Daya Bay measurement of theta_13, and the interpretation of the results of neutrinoless double-beta decay experiments.
S. M. Bilenky; C. Giunti
Method for double-sided processing of thin film transistors
This invention provides methods for fabricating thin film electronic devices with both front- and backside processing capabilities. Using these methods, high temperature processing steps may be carried out during both frontside and backside processing. The methods are well-suited for fabricating back-gate and double-gate field effect transistors, double-sided bipolar transistors and 3D integrated circuits.
Yuan, Hao-Chih (Madison, WI); Wang, Guogong (Madison, WI); Eriksson, Mark A. (Madison, WI); Evans, Paul G. (Madison, WI); Lagally, Max G. (Madison, WI); Ma, Zhenqiang (Middleton, WI)
Microfluidic DNA sample preparation method and device
Manipulation of DNA molecules in solution has become an essential aspect of genetic analyses used for biomedical assays, the identification of hazardous bacterial agents, and in decoding the human genome. Currently, most of the steps involved in preparing a DNA sample for analysis are performed manually and are time, labor, and equipment intensive. These steps include extraction of the DNA from spores or cells, separation of the DNA from other particles and molecules in the solution (e.g. dust, smoke, cell/spore debris, and proteins), and separation of the DNA itself into strands of specific lengths. Dielectrophoresis (DEP), a phenomenon whereby polarizable particles move in response to a gradient in electric field, can be used to manipulate and separate DNA in an automated fashion, considerably reducing the time and expense involved in DNA analyses, as well as allowing for the miniaturization of DNA analysis instruments. These applications include direct transport of DNA, trapping of DNA to allow for its separation from other particles or molecules in the solution, and the separation of DNA into strands of varying lengths.
Krulevitch, Peter A. (Pleasanton, CA); Miles, Robin R. (Danville, CA); Wang, Xiao-Bo (San Diego, CA); Mariella, Raymond P. (Danville, CA); Gascoyne, Peter R. C. (Bellaire, TX); Balch, Joseph W. (Livermore, CA)
Fleet DNA Project (Fact Sheet)
The Fleet DNA Project - designed by the U.S. Department of Energy's National Renewable Energy Laboratory (NREL) in partnership with Oak Ridge National Laboratory - aims to accelerate the evolution of advanced vehicle development and support the strategic deployment of market-ready technologies that reduce costs, fuel consumption, and emissions. At the heart of the Fleet DNA Project is a clearinghouse of medium- and heavy-duty commercial fleet transportation data for optimizing the design of advanced vehicle technologies or for selecting a given technology to invest in. An easy-to-access online database will help vehicle manufacturers and fleets understand the broad operational range for many of today's commercial vehicle vocations.
Electromagnetic Signals from Bacterial DNA
Chemical reactions can be induced at a distance due to the propagation of electromagnetic signals during intermediate chemical stages. Although is is well known at optical frequencies, e.g. photosynthetic reactions, electromagnetic signals hold true for muck lower frequencies. In E. coli bacteria such electromagnetic signals can be generated by electric transitions between energy levels describing electrons moving around DNA loops. The electromagnetic signals between different bacteria within a community is a "wireless" version of intercellular communication found in bacterial communities connected by "nanowires". The wireless broadcasts can in principle be of both the AM and FM variety due to the magnetic flux periodicity in electron energy spectra in bacterial DNA orbital motions.
A. Widom; J. Swain; Y. N. Srivastava; S. Sivasubramanian
Channel plate for DNA sequencing
This invention is a channel plate that facilitates data compaction in DNA sequencing. The channel plate has a length, a width and a thickness, and further has a plurality of channels that are parallel. Each channel has a depth partially through the thickness of the channel plate. Additionally an interface edge permits electrical communication across an interface through a buffer to a deposition membrane surface. 15 figs.
Douthart, R.J.; Crowell, S.L.
Pionic contribution to neutrinoless double beta decay
It is well known that neutrinoless double decay is going to play a crucial role in settling the neutrino properties, which cannot be extracted from the neutrino oscillation data. It is, in particular, expected to settle the absolute scale of neutrino mass and determine whether the neutrinos are Majorana particles, i.e. they coincide with their own antiparticles. In order to extract the average neutrino mass from the data, one must be able to estimate the contribution of all possible high mass intermediate particles. The latter, which occur in practically all extensions of the standard model, can, in principle, be differentiated from the usual mass term, if data from various targets are available. One, however, must first be able to reliably calculate the corresponding nuclear matrix elements. Such calculations are extremely difficult since the effective transition operators are very short ranged. For such operators processes like pionic contributions, which are usually negligible, turn out to be dominant. We study such an effect in a nonrelativistic quark model for the pion and the nucleon.
Vergados, J. D. [Physics Department, University of Ioannina, Ioannina, GR 451 10 (Greece); Theory Division, CERN, Geneva (Switzerland); Faessler, Amand [Institute fuer Theoretische Physik, Universitaet Tuebingen (Germany); Toki, H. [RCNP, Osaka University, Osaka, 567-0047 (Japan)
Double-duct liquid metal magnetohydrodynamic engine
An internal combustion, liquid metal (LM) magnetohydrodynamic (MHD) engine and an alternating current (AC) magnetohydrodynamic generator, are used in combination to provide useful AC electric energy output. The engine design has four pistons and a double duct configuration, with each duct containing sodium potassium liquid metal confined between free pistons located at either end of the duct. The liquid metal is forced to flow back and forth in the duct by the movement of the pistons, which are alternatively driven by an internal combustion process. In the MHD generator, the two LM-MHD ducts pass in close proximity through a Hartmann duct with output transformer. AC power is produced by operating the engine with the liquid metal in the two generator ducts always flowing in counter directions. The amount of liquid metal maintained in the ducts may be varied. This provides a variable stroke length for the pistons. The engine/generator provides variable AC power at variable frequencies that correspond to the power demands of the vehicular propulsion. Also the engine should maintain nearly constant efficiency throughout the range of power usage. Automobiles and trucks could be powered by the invention, with no transmission or power converter devices being required.
Haaland, Carsten M. (Oak Ridge, TN)
An internal combustion, liquid metal (LM) magnetohydrodynamic (MHD) engine and an alternating current (AC) magnetohydrodynamic generator, are used in combination to provide useful AC electric energy output. The engine design has-four pistons and a double duct configuration, with each duct containing sodium potassium liquid metal confined between free pistons located at either end of the duct. The liquid metal is forced to flow back and forth in the duct by the movement of the pistons, which are alternatively driven by an internal combustion process. In the MHD generator, the two LM-MHD ducts pass in close proximity through a Hartmann duct with output transformer. AC power is produced by operating the engine with the liquid metal in the two generator ducts always flowing in counter directions. The amount of liquid metal maintained in the ducts may be varied. This provides a variable stroke length for the pistons. The engine/generator provides variable AC power at variable frequencies that correspond to the power demands of the vehicular propulsion. Also the engine should maintain nearly constant efficiency throughout the range of power usage. Automobiles and trucks could be powered by the invention, with no transmission or power converter devices being required.
First results on neutrinoless double beta decay of Te-130 with the calorimetric cuoricino experiment
Evidence for Neutrinoless Double Beta Decay� arXiv:hep-on �Evidence for neutrinoless double beta decay�- arXiv:hep-Results on Neutrinoless Double Beta Decay of 130 Te with the
Half-lives of Double $\\beta ^+$-decay with Two Neutrinos
Nuclear double $\\beta ^-$-decays with two neutrinos were observed for many years and a systematic law describing the relation between their half-lives and decay energies was also proposed recently [Phys. Rev. C89, 064603 (2014)]. However, double $\\beta ^+$-decay ($\\beta ^+\\beta^+)$ with emission of both two positrons and two neutrinos has not been observed up to date. In this article, we perform a systematic analysis on the candidates of double $\\beta ^+$-decay, based on the 2012 nuclear mass table. Eight nuclei are found to be the good candidates for double $\\beta ^+$-decay and their half-lives are predicted according to the generalization of the systematic law to double $\\beta ^+$-decay. As far as we know, there is no theoretical result on double $\\beta ^+$-decay of nucleus $^{154}Dy$ and our result is the first prediction on this nucleus. This is also the first complete research on eight double $\\beta ^+$-decay candidates based on the available data of nuclear masses. It is expected that the calculated hal...
Ren, Yuejiao
Experimental research of double beta decay of atomic nuclei
Results of several double beta decay experiments, performed with the help of low background crystal scintillators, are presented. In particular, the half-life value of the two-neutrino double beta decay of 116-Cd has been measured as 2.9 10^{19} yr, and the new half-life limit on the neutrinoless double beta decay of 116-Cd has been established as >1.7 10^{23} yr at 90%, which corresponds to a restriction on the neutrino mass <1.7 eV. New half-life bounds on the level of 10^{17}-10^{21} yr were set for double beta processes in 64-Zn, 70-Zn, 106-Cd, 108-Cd, 114-Cd, 136-Ce, 138-Ce, 142-Ce, 160-Gd, 180-W, and 186-W by using low-background CdWO4, GSO, and ZnWO4 crystal scintillators. The claim of discovery of the neutrinoless double beta decay of 76-Ge [Mod. Phys. Lett. A 16 (2001) 2409] was analyzed. The demands of the future high sensitivity double beta decay experiments, aiming to observe the neutrinoless double beta decay or to advance restrictions on the neutrino mass to < 0.01 eV, were considered. Requirements for their sensitivity and discovery potential were formulated. Two projects of double beta experiments with a sensitivity on the level of 10^{26}-10^{27} yr (CAMEO and CARVEL projects) were discussed. Scintillation properties and radioactive contamination of CaWO4, ZnWO4, CdWO4, PbWO4, GSO(Ce), CeF3, yttrium-aluminum garnet doped with neodymium (YAG:Nd) crystal scintillators were studied. Applicability of these scintillators to search for double beta decay was discussed.
F. A. Danevich
Majorons Without Majorana Masses and Neutrinoless Double Beta Decay
We explain excess events near the endpoints of the double beta decay spectra of several elements, using the neutrinoless emission of massless Goldstone bosons. Models with scalars carrying lepton number $-2$ are proposed for this purpose so that ordinary neutrinoless double beta decay is forbidden, and we can raise the scale of global symmetry breaking above the 10 keV scale needed for observable emission of conventional Majorons in double beta decay. The electron spectrum has a different shape, and the rate depends on different nuclear matrix elements, than for the emission of ordinary Majorons.
C. P. Burgess; J. M. Cline
The various mechanisms for neutrinoless double beta decay in gauge theories are reviewed and the present experimental data is used to set limits on physics scenarios beyond the standard model. The positive indications for nonzero neutrino masses in various experiments such as those involving solar, atmospheric and accelerator neutrinos are discussed and it is pointed out how some neutrino mass textures consistent with all data can be tested by the ongoing double beta decay experiments. Finally, the outlook for observable neutrinoless double beta decay signal in grand unified theories is discussed.
Rabindra N. Mohapatra
Increased Upstream Ionization due to Formation of a Double Layer
We report observations that confirm a theoretical prediction that formation of a current-free double layer in a plasma expanding into a chamber of larger diameter is accompanied by an increase in ionization upstream of the double layer. The theoretical model argues that the increased ionization is needed to balance the difference in diffusive losses upstream and downstream of the expansion region. In our expanding helicon source experiments, we find that the upstream plasma density increases sharply at the same antenna frequency at which the double layer appears.
Thakur, S. Chakraborty; Harvey, Z.; Biloiu, I. A.; Hansen, A.; Hardin, R. A.; Przybysz, W. S.; Scime, E. E. [Department of Physics, West Virginia University, Morgantown, West Virginia 26506-6315 (United States)
Electric Field in a Double Layer and the Imparted Momentum
It is shown that the net momentum delivered by the large electric field inside a one-dimensional double layer is zero. This is demonstrated through an analysis of the momentum balance in the double layer at the boundary between the ionosphere and the aurora cavity. For the recently observed double layer in a current-free plasma expanding along a divergent magnetic field, an analysis of the evolution of the radially averaged variables shows that the increase of plasma thrust results from the magnetic-field pressure balancing the plasma pressure in the direction of acceleration, rather than from electrostatic pressure.
Fruchtman, A. [Holon Academic Institute of Technology, 52 Golomb Street, Holon 58102 (Israel)
Superconvergence of period doubling cascade in trapezoid maps
In the symmetric and the asymmetric trapezoid maps, as a slope of the trapezoid is increased, the period doubling cascade occurs and the symbolic sequence of periodic points is the Metropolis-Stein-Stein sequence and the convergence of the onset point of the period 2^m solution to the accumulation point is exponentially fast. We reported these results previously. In this paper, we give the detailed description of the proof on the results. Further, we study the period doubling cascade starting from period p solution and show the superconvergence of the period doubling cascade.
T. Uezu
aligned double-walled carbon: Topics by E-print Network
Reinhard 2 Atomistic Simulations of Double-Walled Carbon Nanotubes (DWCNTs) as Materials Science Websites Summary: Atomistic Simulations of Double-Walled Carbon Nanotubes...
above-threshold nonsequential double: Topics by E-print Network
T-581: Novell Access Manager Java Double Literal Denial of Service...
Vulnerability Report: Novell Access Manager 3.x Oracle FPUpdater Tool IMPACT ASSESSMENT: Medium Discussion: The Double.parseDouble method in Java Runtime Environment in Oracle...
A Search for Neutrinoless Double-Beta Decay with EXO-200 .
??This work presents a search for neutrinoless double beta decay of 136Xe using data from the EXO-200 detector collected between 2011 and 2012. Neutrinoless double� (more)
Slutsky, Simon
Neutrinoless double beta decay in deformed nuclei: its implications in particle and nuclear physics .
??In my thesis, we calculated the Nuclear Matrix Elements (NME) for neutrinoless double beta decay (0??? decay). Neutrinoless double beta decay is a rare nuclear� (more)
Fang, DongLiang
The dynamic interplay between DNA damage and metabolism : the metabolic fate and transport of DNA lesions and novel DNA damage derived from intermediary metabolism
The work presented in this thesis explores two novel and complementary facets of endogenous DNA damage: the development of biomarkers of inflammation based on metabolites of DNA damage products and the formation of DNA ...
Jumpathong, Watthanachai
AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation Proposed Newcatalyst phases onOrganization FY Middle SchoolARM-TR-01468This4t c 5DMSEdrives
Enhancing the DNA Patent Database
Final Report on Award No. DE-FG0201ER63171 Principal Investigator: LeRoy B. Walters February 18, 2008 This project successfully completed its goal of surveying and reporting on the DNA patenting and licensing policies at 30 major U.S. academic institutions. The report of survey results was published in the January 2006 issue of Nature Biotechnology under the title �The Licensing of DNA Patents by US Academic Institutions: An Empirical Survey.� Lori Pressman was the lead author on this feature article. A PDF reprint of the article will be submitted to our Program Officer under separate cover. The project team has continued to update the DNA Patent Database on a weekly basis since the conclusion of the project. The database can be accessed at dnapatents.georgetown.edu. This database provides a valuable research tool for academic researchers, policymakers, and citizens. A report entitled Reaping the Benefits of Genomic and Proteomic Research: Intellectual Property Rights, Innovation, and Public Health was published in 2006 by the Committee on Intellectual Property Rights in Genomic and Protein Research and Innovation, Board on Science, Technology, and Economic Policy at the National Academies. The report was edited by Stephen A. Merrill and Anne-Marie Mazza. This report employed and then adapted the methodology developed by our research project and quoted our findings at several points. (The full report can be viewed online at the following URL: http://www.nap.edu/openbook.php?record_id=11487&page=R1). My colleagues and I are grateful for the research support of the ELSI program at the U.S. Department of Energy.
Walters, LeRoy B.
Characterization of double walled carbon nanotubes-polyvinylidene fluoride nanocomposites
One of the main objectives of this thesis is to disperse double-walled carbon nanotubes (DWNT) in a polyvinylidene fluoride (PVDF) matrix, and to characterize the resulting composite using electrical, thermal, and mechanical characterization...
Almasri, Atheer Mohammad
Double Shell Tank (DST) Monitor and Control Subsystem Specification
This specification revises the performance requirements and provides references to the requisite codes and standards to be applied during design of the Double-Shell Tank (DST) Monitor and Control Subsystem that supports the first phase of Waste Feed Delivery.
Double Shell Tank (DST) Process Waste Sampling Subsystem Specification
This specification establishes the performance requirements and provides references to the requisite codes and standards to be applied to the Double-Shell Tank (DST) Process Waste Sampling Subsystem which supports the first phase of Waste Feed Delivery.
additional double bonds: Topics by E-print Network
such as weak-magnetism and pseudoscalar couplings to the amplitude of neutrinoless double beta decay in the mechanisms of light and heavy Majorana neutrino as well as in that...
Molecular Dynamics Study of the Electrical Double Layer at Silver...
double layer at AgClaqueous electrolyte (KCl) interfaces are presented, accompanied by a new force field and properties of bulk AgCl computed using planewave density functional...
Double beta decay experiments: past and present achievements
A brief history of double beta decay experiments is presented. The best currently running experiments (NEMO-3 and CUORICINO) and their latest results are described. The best measurements and limits for the $2\
Alexander Barabash
Double Beta Decay: Historical Review of 75 Years of Research
Main achievements during 75 years of research on double beta decay have been reviewed. The existing experimental data have been presented and the capabilities of the next-generation detectors have been demonstrated.
Superconductivity in Bundles of Double-Wall Carbon Nanotubes
We present electrical and thermal specific heat measurements that show superconductivity in double-wall carbon nanotube (DWCNT) bundles. Clear evidence, comprising a resistance drop as a function of temperature, magnetoresistance ...
Shi, Wu
Double beta decay experiments: beginning of a new era
The review of current experiments on search and studying of double beta decay processes is done. Results of the most sensitive experiments are discussed and values of modern limits on effective Majorana neutrino mass ($) are given. New results on two neutrino double beta decay are presented. The special attention is given to new current experiments with mass of studied isotopes more than 100 kg, EXO--200 and KamLAND--Zen. These experiments open a new era in research of double beta decay. In the second part of the review prospects of search for neutrinoless double beta decay in new experiments with sensitivity to $$ at the level of $\\sim 0.01-0.1$ eV are discussed. Parameters and characteristics of the most perspective projects (CUORE, GERDA, MAJORANA, SuperNEMO, EXO, KamLAND--Zen, SNO+) are given.
Bounds on Compositeness from Neutrinoless Double Beta Decay
Assuming the existence of a heavy Majorana neutral particle arising from a composite model scenario we discuss the constraints imposed by present experimental limits of half-life neutrinoless double beta decay $(0\
Orlando Panella; Yogendra N. Srivastava
Neutrinoless double beta decay, solar neutrinos and mass scales
We obtain bounds for the neutrino masses by combining atmospheric and solar neutrino data with the phenomenology of neutrinoless double beta decay where hypothetical values of || are envisaged from future 0\
Per Osland; Geir Vigdel
Classical cutoffs for laser-induced nonsequential double ionization
Classical cutoffs for the momenta of electrons ejected in laser-induced nonsequential double ionization are derived for the recollision-impact-ionization scenario. Such simple cutoff laws can aid in the interpretation of the observed electron spectra.
Milosevic, D.B. [Faculty of Science, University of Sarajevo, Zmaja od Bosne 35, 71000 Sarajevo (Bosnia and Herzegowina); Max-Born-Institut, Max-Born-Strasse 2a, 12489 Berlin (Germany); Becker, W. [Max-Born-Institut, Max-Born-Strasse 2a, 12489 Berlin (Germany)
Comment on "Evidence for Neutrinoless Double Beta Decay"
We comment on the recent claim for the experimental observation of neutrinoless double-beta decay. We discuss several limitations in the analysis provided in that paper and conclude that there is no basis for the presented claim.
C. E. Aalseth; F. T. Avignone III; A. Barabash; F. Boehm; R. L. Brodzinski; J. I. Collar; P. J. Doe; H. Ejiri; S. R. Elliott; E. Fiorini; R. J. Gaitskell; G. Gratta; R. Hazama; K. Kazkaz; G. S. King III; R. T. Kouzes; H. S. Miley; M. K. Moe; A. Morales; J. Morales; A. Piepke; R. G. H. Robertson; W. Tornow; P. Vogel; R. A. Warner; J. F. Wilkerson
New expectations and uncertainties on Neutrinoless Double Beta Decay
The discovery of neutrino oscillations and its implication that neutrinos have mass have boosted the importance of neutrinoless double beta decay. Neutrinoless double beta decay offers unique chances to investigate the nature of the neutrino mass term, giving also information on the absolute scale and the mass hierarchy, assuming that neutrinos are Majorana particles. We study the Majorana Effective Mass, i. e. the crucial parameter that regulates the rate of the neutrinoless double beta decay due to light neutrino exchange. We update the previous estimations of this parameter, using the most recent data analysis, phase space factors and nuclear matrix elements. We evaluate the impact of the quenching in the nuclear medium of the axial vector coupling constant, as discussed by Iachello and collaborators. We provide estimations of the sensitivity of recent and future neutrinoless double beta decay experiments in terms of the Majorana Effective Mass. Finally, we discuss the possibility of taking advantage of th...
Dell'Oro, Stefano; Vissani, Francesco
Improved cell cycle synchronization and chromosome doubling methods in cotton
Cotton is an economically important crop in the U.S., but is relatively poorly characterized genetically. Unlike other, more researched crops, it is lacking highly effective methods for consistent cell cycle manipulation and chromosome doubling...
Halfmann, Randal
EA-1136: Double Tracks Test Site, Nye County, Nevada
Broader source: Energy.gov [DOE]
This EA evaluates the environmental impacts of the proposal for the U.S. Department of Energy Nevada Operations Office to conduct environmental restoration operations at the Double Tracks test site...
Comparing Control Constructs by Typing Double-barrelled CPS Transforms
Comparing Control Constructs by Typing Double-barrelled CPS Transforms Hayo Thielecke School Kingdom Copyright 2000 ACM 0-89791-88-6/97/05 ..$5.00 the bare essentials of labelling and jumping, so
Thielecke, Hayo
Electrochemical Double-Layer Capacitors Using Carbon Nanotube Electrode Structures
The structure and behavior of the electrical double-layer capacitor (EDLC) are described. The use of activated carbon electrodes is discussed and the limitations on voltage and accessible surface area are presented. Metrics ...
Schindall, Joel E.
Electrochemical Double Layered Capacitor Development and Implementation System
Electrochemical Double Layered Capacitors (EDLC's) are becoming a more popular topic of research for hybrid power systems, especially vehicles. They are known for their high power density, high cycle life, low internal resistance, and wider...
Strunk, Gavin
DNA-guided nanoparticle assemblies
In some embodiments, DNA-capped nanoparticles are used to define a degree of crystalline order in assemblies thereof. In some embodiments, thermodynamically reversible and stable body-centered cubic (bcc) structures, with particles occupying <.about.10% of the unit cell, are formed. Designs and pathways amenable to the crystallization of particle assemblies are identified. In some embodiments, a plasmonic crystal is provided. In some aspects, a method for controlling the properties of particle assemblages is provided. In some embodiments a catalyst is formed from nanoparticles linked by nucleic acid sequences and forming an open crystal structure with catalytically active agents attached to the crystal on its surface or in interstices.
Gang, Oleg; Nykypanchuk, Dmytro; Maye, Mathew; van der Lelie, Daniel
Human Genome Research: Decoding DNA
AFDC Printable Version Share this resource Send a link to EERE: Alternative Fuels Data Center Home Page to someone by E-mail Share EERE: Alternative Fuels Data Center Home Page on Facebook Tweet about EERE: Alternative Fuels Data Center Home Page on Twitter Bookmark EERE:1 First Use of Energy for All Purposes (Fuel and Nonfuel),Feet) Year Jan Feb Mar Apr MayAtmospheric Optical Depth7-1D: Vegetation ProposedUsingFun withconfinement plasmas inPortalAllBPA addressesHowHughHumanDecoding DNA
Assembling semiconductor nanocomposites using DNA replication technologies.
Deoxyribonucleic acid (DNA) molecules represent Nature's genetic database, encoding the information necessary for all cellular processes. From a materials engineering perspective, DNA represents a nanoscale scaffold with highly refined structure, stability across a wide range of environmental conditions, and the ability to interact with a range of biomolecules. The ability to mass-manufacture functionalized DNA strands with Angstrom-level resolution through DNA replication technology, however, has not been explored. The long-term goal of the work presented in this report is focused on exploiting DNA and in vitro DNA replication processes to mass-manufacture nanocomposite materials. The specific objectives of this project were to: (1) develop methods for replicating DNA strands that incorporate nucleotides with ''chemical handles'', and (2) demonstrate attachment of nanocrystal quantum dots (nQDs) to functionalized DNA strands. Polymerase chain reaction (PCR) and primer extension methodologies were used to successfully synthesize amine-, thiol-, and biotin-functionalized DNA molecules. Significant variability in the efficiency of modified nucleotide incorporation was observed, and attributed to the intrinsic properties of the modified nucleotides. Noncovalent attachment of streptavidin-coated nQDs to biotin-modified DNA synthesized using the primer extension method was observed by epifluorescence microscopy. Data regarding covalent attachment of nQDs to amine- and thiol-functionalized DNA was generally inconclusive; alternative characterization tools are necessary to fully evaluate these attachment methods. Full realization of this technology may facilitate new approaches to manufacturing materials at the nanoscale. In addition, composite nQD-DNA materials may serve as novel recognition elements in sensor devices, or be used as diagnostic tools for forensic analyses. This report summarizes the results obtained over the course of this 1-year project.
Heimer, Brandon W.; Crown, Kevin K.; Bachand, George David
Band Tunneling through Double Barrier in Bilayer Graphene
By taking into account the full four band energy spectrum, we calculate the transmission probability and conductance of electrons across symmetric and asymmetric double potential barrier with a confined interlayer potential difference in bilayer graphene. For energies less than the interlayer coupling \\gamma_{1}, E \\gamma_{1}, we obtain four possible ways for transmission resulting from the two propagating modes. We compute the associated transmission probabilities as well as their contribution to the conductance, study the effect of the double barrier geometry.
Hasan A. Alshehab; Hocine Bahlouli; Abderrahim El Mouhafid; Ahmed Jellal
Neutrinoless Double Beta Decay with Negligible Neutrino Mass
If the electron neutrino has an effective nonzero Majorana mass, then neutrinoless double beta decay will occur. However, the latter is possible also with a negligible neutrino mass. We show how this may happen in a simple model of scalar diquarks and dileptons. This possibility allows neutrino masses to be small and hierarchical, without conflicting with the possible experimental evidence of neutrinoless double beta decay.
Biswajoy Brahmachari; Ernest Ma
Overconstrained estimates of neutrinoless double beta decay within the QRPA
Estimates of nuclear matrix elements for neutrinoless double beta decay (0nu2beta) based on the quasiparticle random phase approximations (QRPA) are affected by theoretical uncertainties, which can be substantially reduced by fixing the unknown strength parameter g_pp of the residual particle-particle interaction through one experimental constraint - most notably through the two-neutrino double beta decay (2nu2beta) lifetime. However, it has been noted that the g_pp adjustment via 2\
Amand Faessler; Gianluigi Fogli; Eligio Lisi; Vadim Rodin; Anna Maria Rotunno; Fedor Simkovic
Effect of nuclear deformation on double beta decay
The existing ways of accounting for deformation in recent calculations of neutrinoless double beta decay matrix elements are discussed. From an analysis of relevant experimental data it is argued that only {sup 150}Nd reveals convincing evidences of strong static deformation, which should eventually be taken into account in QRPA calculations. A proposal which allows in principle to measure the neutrino less double beta decay Fermi matrix element is briefly described.
Rodin, Vadim [Institute fuer Theoretische Physik der Universitaet Tuebingen, D-72076 Tuebingen (Germany)
Optical Recognition of Converted DNA Nucleotides for Single-Molecule DNA
Optical Recognition of Converted DNA Nucleotides for Single-Molecule DNA Sequencing Using Nanopore among individual nucleotides (nt). The system must be capable of differentiating among the four bases
Chemical biology of mutagenesis and DNA repair: cellular responses to DNA alkylation
The reaction of DNA-damaging agents with the genome results in a plethora of lesions, commonly referred to as adducts. Adducts may cause DNA to mutate, they may represent the chemical precursors of lethal events and they ...
Shrivastav, Nidhi
Nucleotide capacitance calculation for DNA sequencing
Using a first-principles linear response theory, the capacitance of the DNA nucleotides, adenine, cytosine, guanine and thymine, are calculated. The difference in the capacitance between the nucleotides is studied with respect to conformational distortion. The result suggests that although an alternate current capacitance measurement of a single-stranded DNA chain threaded through a nano-gap electrodes may not sufficient to be used as a stand alone method for rapid DNA sequencing, the capacitance of the nucleotides should be taken into consideration in any GHz-frequency electric measurements and may also serve as an additional criterion for identifying the DNA sequence.
Lu, Jun-Qiang [ORNL; Zhang, Xiaoguang [ORNL
We assess how well next generation neutrinoless double beta decay and normal neutrino beta decay experiments can answer four fundamental questions. 1) If neutrinoless double beta decay searches do not detect a signal, and if the spectrum is known to be inverted hierarchy, can we conclude that neutrinos are Dirac particles? 2) If neutrinoless double beta decay searches are negative and a next generation ordinary beta decay experiment detects the neutrino mass scale, can we conclude that neutrinos are Dirac particles? 3) If neutrinoless double beta decay is observed with a large neutrino mass element, what is the total mass in neutrinos? 4) If neutrinoless double beta decay is observed but next generation beta decay searches for a neutrino mass only set a mass upper limit, can we establish whether the mass hierarchy is normal or inverted? We base our answers on the expected performance of next generation neutrinoless double beta decay experiments and on simulations of the accuracy of calculations of nuclear matrix elements.
John N. Bahcall; Hitoshi Murayama; Carlos Pena-Garay
Double-core excitations in formamide can be probed by X-ray double-quantum-coherence spectroscopy
Yu Zhang, Daniel Healion, Jason D. Biggs, and Shaul Mukamel Citation: J. Chem. Phys. 138, 144301 be probed by X-ray double-quantum-coherence spectroscopy Yu Zhang, Daniel Healion, Jason D. Biggs, and Shaul
Mukamel, Shaul
A DNA Based Implementation of an Evolutionary Search for Good Encodings for DNA Computation
lelism, capacity, and power. This potential, however, is limited by the constraints imposed by the DNA chemistry 1]. Adleman 2] introduced a way to solve combina- torial optimization problems with DNA- mental reaction in DNA based computation is hydro- gen bonding between Watson-Crick complement base pairs
Deaton, Russell J.
DNA Repair Alterations in Children With Pediatric Malignancies: Novel Opportunities to Identify Patients at Risk for High-Grade Toxicities
Purpose: To evaluate, in a pilot study, the phosphorylated H2AX ({gamma}H2AX) foci approach for identifying patients with double-strand break (DSB) repair deficiencies, who may overreact to DNA-damaging cancer therapy. Methods and Materials: The DSB repair capacity of children with solid cancers was analyzed compared with that of age-matched control children and correlated with treatment-related normal-tissue responses (n = 47). Double-strand break repair was investigated by counting {gamma}H2AX foci in blood lymphocytes at defined time points after irradiation of blood samples. Results: Whereas all healthy control children exhibited proficient DSB repair, 3 children with tumors revealed clearly impaired DSB repair capacities, and 2 of these repair-deficient children developed life-threatening or even lethal normal-tissue toxicities. The underlying mutations affecting regulatory factors involved in DNA repair pathways were identified. Moreover, significant differences in mean DSB repair capacity were observed between children with tumors and control children, suggesting that childhood cancer is based on genetic alterations affecting DSB repair function. Conclusions: Double-strand break repair alteration in children may predispose to cancer formation and may affect children's susceptibility to normal-tissue toxicities. Phosphorylated H2AX analysis of blood samples allows one to detect DSB repair deficiencies and thus enables identification of children at risk for high-grade toxicities.
Ruebe, Claudia E., E-mail: email@example.com [Department of Radiation Oncology, Saarland University, Homburg/Saar (Germany); Fricke, Andreas; Schneider, Ruth; Simon, Karin; Kuehne, Martin; Fleckenstein, Jochen [Department of Radiation Oncology, Saarland University, Homburg/Saar (Germany); Graeber, Stefan [Institute of Medical Biometrics, Epidemiology and Medical Informatics, Saarland University, Homburg/Saar (Germany); Graf, Norbert [Department of Pediatric Hematology and Oncology, Saarland University, Homburg/Saar (Germany); Ruebe, Christian [Department of Radiation Oncology, Saarland University, Homburg/Saar (Germany)
Next Generation Neutrinoless Double -Decay Experiments Andreas Piepke, R.G. Hamish Robertson
of this ambitious program...". The Long Range Plan further states: "... neutrinoless double beta decay experiments
Dynamics and control of DNA sequence amplification
DNA amplification is the process of replication of a specified DNA sequence in vitro through time-dependent manipulation of its external environment. A theoretical framework for determination of the optimal dynamic operating conditions of DNA amplification reactions, for any specified amplification objective, is presented based on first-principles biophysical modeling and control theory. Amplification of DNA is formulated as a problem in control theory with optimal solutions that can differ considerably from strategies typically used in practice. Using the Polymerase Chain Reaction as an example, sequence-dependent biophysical models for DNA amplification are cast as control systems, wherein the dynamics of the reaction are controlled by a manipulated input variable. Using these control systems, we demonstrate that there exists an optimal temperature cycling strategy for geometric amplification of any DNA sequence and formulate optimal control problems that can be used to derive the optimal temperature profile. Strategies for the optimal synthesis of the DNA amplification control trajectory are proposed. Analogous methods can be used to formulate control problems for more advanced amplification objectives corresponding to the design of new types of DNA amplification reactions.
Marimuthu, Karthikeyan [Department of Chemical Engineering and Center for Advanced Process Decision-Making, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 (United States); Chakrabarti, Raj, E-mail: firstname.lastname@example.com, E-mail: firstname.lastname@example.com [Department of Chemical Engineering and Center for Advanced Process Decision-Making, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213 (United States); Division of Fundamental Research, PMC Advanced Technology, Mount Laurel, New Jersey 08054 (United States)
DNA Motif Representation with Nucleotide Dependency
DNA Motif Representation with Nucleotide Dependency Francis Chin1 and Henry Leung1 1 Department, these representations cannot model biological binding sites well because they fail to capture nucleotide interdependence. It has been pointed out by many researchers that the nucleotides of the DNA binding site cannot
Chin, Francis Y.L.
Carbon Nanotube DNA Sensor and Sensing Mechanism
Carbon Nanotube DNA Sensor and Sensing Mechanism Xiaowu Tang,*,, Sarunya Bansaruntip, Nozomi; Revised Manuscript Received June 7, 2006 ABSTRACT We report the fabrication of single-walled carbon nanotube (SWNT) DNA sensors and the sensing mechanism. The simple and generic protocol for label
Le Roy, Robert J.
Recombinant DNA encoding a desulfurization biocatalyst
This invention relates to a recombinant DNA molecule containing a gene or genes which encode a biocatalyst capable of desulfurizing a fossil fuel which contains organic sulfur molecules. For example, the present invention encompasses a recombinant DNA molecule containing a gene or genes of a strain of Rhodococcus rhodochrous. 13 figs.
Rambosek, J.; Piddington, C.S.; Kovacevich, B.R.; Young, K.D.; Denome, S.A.
Time-resolved measurements of double layer evolution in expanding plasma
Observations in steady-state plasmas confirm predictions that formation of a current-free double layer in a plasma expanding into a chamber of larger diameter is accompanied by an increase in ionization upstream of the double layer. The upstream plasma density increases sharply at the same driving frequency at which a double layer appears. For driving frequencies at which no double layer appears, large electrostatic instabilities are observed. Time-resolved measurements in pulsed discharges indicate that the double layer initially forms for all driving frequencies. However, for particularly strong double layers, instabilities appear early in the discharge and the double layer collapses.
Scime, E. E.; Biloiu, I. A.; Carr, J. Jr.; Thakur, S. Chakraborty; Galante, M.; Hansen, A.; Houshmandyar, S.; Keesee, A. M.; McCarren, D.; Sears, S. [Department of Physics, West Virginia University, Morgantown, West Virginia 26506 (United States); Biloiu, C. [Varian Associates, Gloucester, Massachusetts 01930 (United States); Sun, X. [Tri-Alpha Corporation, Foothill Ranch, California 92610 (United States)
Electric DNA chips for determination of pathogenic microorganisms
Electric DNA chips for determination of pathogenic microorganisms Yanling Liu Doctoral thesis Liu (2008): Electric DNA chips for determination of pathogenic microorganisms. School of Biotechnology, Royal Institute of Technology (KTH), Stockholm, Sweden Abstract Silicon-based electric DNA chip arrays
Enfors, Sven-Olof
Mitochondrial DNA phylogenetic analysis of Myrmecolacidae (Insecta: Strepsiptera) va
Guinea are determined. Methods were developed for extraction, amplification, and sequencing of mitochondrial DNA (mtDNA) from Strepsiptera. High-resolution mtDNA sequences obtained by these methods were used in the phylogenetic analysis of Myrmecolacidae...
Halbert, Natalie Rose
Single molecule analysis of DNA electrophoresis in microdevices
Given that current electrophoresis technology is inadequate for mapping large O[100 kilobasepair] DNA, several promising lab-on-chip designs for DNA mapping have been recently proposed that require either 1) a DNA molecule ...
Randall, Greg C
Flow cytometric detection method for DNA samples
Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM.TM. on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA.TM., on the 5' end.
Nasarabadi,Shanavaz (Livermore, CA); Langlois, Richard G. (Livermore, CA); Venkateswaran, Kodumudi S. (Round Rock, TX)
Atomic force microscopy of biochemically tagged DNA
Small fragments of DNA of known length were made with the polymerase chain reaction. These fragments had biotin molecules covalently attached at their ends. They were subsequently labeled with a chimeric protein fusion between streptavidin and two immunoglobulin G-binding domains of staphyloccocal protein A. This tetrameric species was expected to bind up to four DNA molecules via their attached biotin moieties. The DNA-protein complex was deposited on mica and imaged with an atomic force microscope. The images revealed the protein chimera at the expected location at the ends of the strands of DNA as well as the expected dimers, trimers, and tetramers of DNA bound to a single protein. 25 refs., 5 figs.
Ogletree, D.F.; Kolbe, W.; Spengler, S.; Salmeron, M. (Lawrence Berkeley Laboratory, CA (United States)); Hansma, H.G.; Bezanilla, M. (Univ. of California, Santa Barbara (United States)); Sano, T.; Smith, C.S.; Cantor, C.R. (Boston Univ., MA (United States))
Storing data encoded DNA in living organisms
Current technologies allow the generation of artificial DNA molecules and/or the ability to alter the DNA sequences of existing DNA molecules. With a careful coding scheme and arrangement, it is possible to encode important information as an artificial DNA strand and store it in a living host safely and permanently. This inventive technology can be used to identify origins and protect R&D investments. It can also be used in environmental research to track generations of organisms and observe the ecological impact of pollutants. Today, there are microorganisms that can survive under extreme conditions. As well, it is advantageous to consider multicellular organisms as hosts for stored information. These living organisms can provide as memory housing and protection for stored data or information. The present invention provides well for data storage in a living organism wherein at least one DNA sequence is encoded to represent data and incorporated into a living organism.
Wong; Pak C. (Richland, WA), Wong; Kwong K. (Sugar Land, TX), Foote; Harlan P. (Richland, WA)
Disclosed herein are two methods for rapid multiplex analysis to determine the presence and identity of target DNA sequences within a DNA sample. Both methods use reporting DNA sequences, e.g., modified conventional Taqman.RTM. probes, to combine multiplex PCR amplification with microsphere-based hybridization using flow cytometry means of detection. Real-time PCR detection can also be incorporated. The first method uses a cyanine dye, such as, Cy3.TM., as the reporter linked to the 5' end of a reporting DNA sequence. The second method positions a reporter dye, e.g., FAM, on the 3' end of the reporting DNA sequence and a quencher dye, e.g., TAMRA, on the 5' end.
Nasarabadi, Shanavaz (Livermore, CA); Langlois, Richard G. (Livermore, CA); Venkateswaran, Kodumudi S. (Livermore, CA)
Crystal Structure of the Chromodomain Helicase DNA-binding Protein 1 (Chd1) DNA-binding Domain in Complex with DNA
Chromatin remodelers are ATP-dependent machines that dynamically alter the chromatin packaging of eukaryotic genomes by assembling, sliding, and displacing nucleosomes. The Chd1 chromatin remodeler possesses a C-terminal DNA-binding domain that is required for efficient nucleosome sliding and believed to be essential for sensing the length of DNA flanking the nucleosome core. The structure of the Chd1 DNA-binding domain was recently shown to consist of a SANT and SLIDE domain, analogous to the DNA-binding domain of the ISWI family, yet the details of how Chd1 recognized DNA were not known. Here we present the crystal structure of the Saccharomyces cerevisiae Chd1 DNA-binding domain in complex with a DNA duplex. The bound DNA duplex is straight, consistent with the preference exhibited by the Chd1 DNA-binding domain for extranucleosomal DNA. Comparison of this structure with the recently solved ISW1a DNA-binding domain bound to DNA reveals that DNA lays across each protein at a distinct angle, yet contacts similar surfaces on the SANT and SLIDE domains. In contrast to the minor groove binding seen for Isw1 and predicted for Chd1, the SLIDE domain of the Chd1 DNA-binding domain contacts the DNA major groove. The majority of direct contacts with the phosphate backbone occur only on one DNA strand, suggesting that Chd1 may not strongly discriminate between major and minor grooves.
Sharma A.; Heroux A.; Jenkins K. R.; Bowman G. D.
Double-strand DNA-templated formation of copper nanoparticles as fluorescent probe for label free nuclease enzyme detection
cleavage activity. Thus, a label-free strategy for sensitive detection of nuclease has been developed B.V. All rights reserved. 1. Introduction As promising substitutes for organic dyes and quantum dots epithelial cervical cancer cells (HeLa cells) using fluorescent gold nanoparticles which emitted a stable
Tan, Weihong
Systematic decomposition of the neutrinoless double beta decay operator
We discuss the systematic decomposition of the dimension nine neutrinoless double beta decay operator, focusing on mechanisms with potentially small contributions to neutrino mass, while being accessible at the LHC. We first provide a (d=9 tree-level) complete list of diagrams for neutrinoless double beta decay. From this list one can easily recover all previously discussed contributions to the neutrinoless double beta decay process, such as the celebrated mass mechanism or "exotics", such as contributions from left-right symmetric models, R-parity violating supersymmetry and leptoquarks. More interestingly, however, we identify a number of new possibilities which have not been discussed in the literature previously. Contact to earlier works based on a general Lorentz-invariant parametrisation of the neutrinoless double beta decay rate is made, which allows, in principle, to derive limits on all possible contributions. We furthermore discuss possible signals at the LHC for mediators leading to the short-range part of the amplitude with one specific example. The study of such contributions would gain particular importance if there were a tension between different measurements of neutrino mass such as coming from neutrinoless double beta decay and cosmology or single beta decay.
Florian Bonnet; Martin Hirsch; Toshihiko Ota; Walter Winter
Double wall vacuum tubing and method of manufacture
An evacuated double wall tubing is shown together with a method for the manufacture of such tubing which includes providing a first pipe of predetermined larger diameter and a second pipe having an O.D. substantially smaller than the I.D. of the first pipe. An evacuation opening is then in the first pipe. The second pipe is inserted inside the first pipe with an annular space therebetween. The pipes are welded together at one end. A stretching tool is secured to the other end of the second pipe after welding. The second pipe is then prestressed mechanically with the stretching tool an amount sufficient to prevent substantial buckling of the second pipe under normal operating conditions of the double wall pipe. The other ends of the first pipe and the prestressed second pipe are welded together, preferably by explosion welding, without the introduction of mechanical spacers between the pipes. The annulus between the pipes is evacuated through the evacuation opening, and the evacuation opening is finally sealed. The first pipe is preferably of steel and the second pipe is preferably of titanium. The pipes may be of a size and wall thickness sufficient for the double wall pipe to be structurally load bearing or may be of a size and wall thickness insufficient for the double wall pipe to be structurally load bearing, and the double wall pipe positioned with a sliding fit inside a third pipe of a load-bearing size.
Stahl, Charles R. (Scotia, NY); Gibson, Michael A. (Houston, TX); Knudsen, Christian W. (Houston, TX)
ap dna endonuclease: Topics by E-print Network
specificity Raines, Ronald T. 2 A DNA and restriction enzyme implementation of Turing Ma (Turing machines; Universal Turing machines; recombinant DNA; nonpalindromic...
adenovirus dna sequences: Topics by E-print Network
description of the given DNA string in terms of a smaller set of distinct domain labels. This yields a minimal domain description of a given DNA sequence, significantly...
Minor Groove Deformability of DNA: A Molecular Dynamics Free...
Minor Groove Deformability of DNA: A Molecular Dynamics Free EnergySimulation Study. Minor Groove Deformability of DNA: A Molecular Dynamics Free EnergySimulation Study. Abstract:...
Electrochemical Branched-DNA Assay for Polymerase Chain Reaction...
Branched-DNA Assay for Polymerase Chain Reaction-Free Detection and Quantification of Oncogenes in Messenger RNA Electrochemical Branched-DNA Assay for Polymerase Chain...
adapting dna microarray: Topics by E-print Network
Gene Expression Analysis & R Tutorial 12;DNA Microarrays An R Tutorial Functional Genomics Qiu, Weigang 3 Research tools known as DNA microarrays are Biology and Medicine...
archived dna microarrays: Topics by E-print Network
Constraint of DNA on Functionalized Graphene Improves Its Biostability...
Constraint of DNA on Functionalized Graphene Improves Its Biostability and Specificity. Constraint of DNA on Functionalized Graphene Improves Its Biostability and Specificity....
CIRCUMSTELLAR ABSORPTION IN DOUBLE DETONATION TYPE Ia SUPERNOVAE
Upon formation, degenerate He core white dwarfs are surrounded by a radiative H-rich layer primarily supported by ideal gas pressure. In this Letter, we examine the effect of this H-rich layer on mass transfer in He+C/O double white dwarf binaries that will eventually merge and possibly yield a Type Ia supernova (SN Ia) in the double detonation scenario. Because its thermal profile and equation of state differ from the underlying He core, the H-rich layer is transferred stably onto the C/O white dwarf prior to the He core's tidal disruption. We find that this material is ejected from the binary system and sweeps up the surrounding interstellar medium hundreds to thousands of years before the SN Ia. The close match between the resulting circumstellar medium profiles and values inferred from recent observations of circumstellar absorption in SNe Ia gives further credence to the resurgent double detonation scenario.
Shen, Ken J. [Lawrence Berkeley National Laboratory, 1 Cyclotron Road, Berkeley, CA 94720 (United States); Guillochon, James [Department of Astronomy and Astrophysics, University of California, Santa Cruz, CA 95064 (United States); Foley, Ryan J., E-mail: firstname.lastname@example.com [Harvard-Smithsonian Center for Astrophysics, 60 Garden Street, Cambridge, MA 02138 (United States)
Heavy Sterile Neutrinos and Neutrinoless Double Beta Decay
Sterile neutrinos of mass up to a few tens of TeV can saturate the present experimental bound of neutrinoless double beta decay process. Due to the updated nuclear matrix elements, the bound on mass and mixing angle is now improved by one order of magnitude. We have performed a detailed analysis of neutrinoless double beta decay for the minimal Type I seesaw scenario. We have shown that in spite of the naive expectation that the light neutrinos give the dominant contribution, sterile neutrinos can saturate the present experimental bound of neutrinoless double beta decay process. However, in order to be consistent with radiative stability of light neutrino masses, the mass scale of sterile neutrinos should be less than 10 GeV.
Manimala Mitra; Goran Senjanovic; Francesco Vissani
Preheat of radiative shock in double-shell ignition targets
For the double-shell ignition target, the nonuniform preheat of the inner shell by high-energy x rays, especially the M-band line radiation and L-shell radiation from the Au hohlraum, aggravates the hydrodynamic instability that causes shell disruption. In this paper, for the first time, we propose another preheating mechanism due to the radiative shock formed in the CH foam, and also confirm and validate such preheat of radiative shock by numerical results. We also give an estimate of the improved double-shell in which the CH foam is replaced by the metallic foam to mitigate the hydrodynamic instabilities, and find that the radiative shock formed in the metallic foam produces a much stronger radiation field to preheat the inner shell, which plays a role in better controlling the instabilities. In double-shells, the preheat of radiative shock, as a potential effect on the instabilities, should be seriously realized and underlined.
Li, J. W.; He, X. T. [Key Lab of High Energy Density Physics Simulation, Center for Applied Physics and Technology, Peking University, Beijing 100871 (China) [Key Lab of High Energy Density Physics Simulation, Center for Applied Physics and Technology, Peking University, Beijing 100871 (China); Institute of Applied Physics and Computational Mathematics, P.O. Box 8009, Beijing 100094 (China); Pei, W. B.; Li, J. H.; Zheng, W. D.; Zhu, S. P. [Institute of Applied Physics and Computational Mathematics, P.O. Box 8009, Beijing 100094 (China)] [Institute of Applied Physics and Computational Mathematics, P.O. Box 8009, Beijing 100094 (China); Kang, W. [Key Lab of High Energy Density Physics Simulation, Center for Applied Physics and Technology, Peking University, Beijing 100871 (China)] [Key Lab of High Energy Density Physics Simulation, Center for Applied Physics and Technology, Peking University, Beijing 100871 (China)
Q value of the 100Mo Double-Beta Decay
Penning trap measurements using mixed beams of 100Mo - 100Ru and 76Ge - 76Se have been utilized to determine the double-beta decay Q-values of 100Mo and 76Ge with uncertainties less than 200 eV. The value for 76Ge, 2039.04(16) keV is in agreement with the published SMILETRAP value. The new value for 100Mo, 3034.40(17) keV is 30 times more precise than the previous literature value, sufficient for the ongoing neutrinoless double-beta decay searches in 100Mo. Moreover, the precise Q-value is used to calculate the phase-space integrals and the experimental nuclear matrix element of double-beta decay.
S. Rahaman; V. -V. Elomaa; T. Eronen; J. Hakala; A. Jokinen; J. Julin; A. Kankainen; A. Saastamoinen; J. Suhonen; C. Weber; J. �yst�
Short-range correlations and neutrinoless double beta decay
In this work we report on the effects of short-range correlations upon the matrix elements of neutrinoless double beta decay. We focus on the calculation of the matrix elements of the neutrino-mass mode of neutrinoless double beta decays of 48Ca and 76Ge. The nuclear-structure components of the calculation, that is the participant nuclear wave functions, have been calculated in the shell-model scheme for 48Ca and in the proton-neutron quasiparticle random-phase approximation (pnQRPA) scheme for 76Ge. We compare the traditional approach of using the Jastrow correlation function with the more complete scheme of the unitary correlation operator method (UCOM). Our results indicate that the Jastrow method vastly exaggerates the effects of short-range correlations on the neutrinoless double beta decay nuclear matrix elements.
M. Kortelainen; O. Civitarese; J. Suhonen; J. Toivanen
Double-Fock Superposition Interferometry for Differential Diagnosis of Decoherence
Interferometric signals are degraded by decoherence, which encompasses dephasing, mixing and any distinguishing which-path information. These three paradigmatic processes are fundamentally different, but, for coherent, single-photon and $N00N$-states, they degrade interferometric visibility in the very same way, which impedes the diagnosis of the cause for reduced visibility in a single experiment. We introduce a versatile formalism for many-boson interferometry based on double-sided Feynman diagrams, which we apply to a protocol for differential decoherence diagnosis: Twin-Fock states |N,N> with $N \\ge 2$ reveal to which extent decoherence is due to path distinguishability or to mixing, while double-Fock superpositions $|N:M> = (|N,M> + |M,N>)/\\sqrt{2} $ with $N > M >0$ additionally witness the degree of dephasing. Hence, double-Fock superposition interferometry permits the differential diagnosis of decoherence processes in a single experiment, indispensable for the assessment of interferometers.
Malte C. Tichy; Young-Sik Ra; Hyang-Tag Lim; Clemens Gneiting; Yoon-Ho Kim; Klaus M�lmer
Design of a combinatorial dna microarray for protein-dnainteraction studies
Background: Discovery of precise specificity oftranscription factors is an important step on the way to understandingthe complex mechanisms of gene regulation in eukaryotes. Recently,doublestranded protein-binding microarrays were developed as apotentially scalable approach to tackle transcription factor binding siteidentification. Results: Here we present an algorithmic approach toexperimental design of a microarray that allows for testing fullspecificity of a transcription factor binding to all possible DNA bindingsites of a given length, with optimally efficient use of the array. Thisdesign is universal, works for any factor that binds a sequence motif andis not species-specific. Furthermore, simulation results show that dataproduced with the designed arrays is easier to analyze and would resultin more precise identification of binding sites. Conclusion: In thisstudy, we present a design of a double stranded DNA microarray forprotein-DNA interaction studies and show that our algorithm allowsoptimally efficient use of the arrays for this purpose. We believe such adesign will prove useful for transcription factor binding siteidentification and other biological problems.
Mintseris, Julian; Eisen, Michael B.
Exploring the common molecular basis for the universal DNA mutation bias: Revival of Loewdin mutation model
Highlights: {yields} There exists a universal G:C {yields} A:T mutation bias in three domains of life. {yields} This universal mutation bias has not been sufficiently explained. {yields} A DNA mutation model proposed by Loewdin 40 years ago offers a common explanation. -- Abstract: Recently, numerous genome analyses revealed the existence of a universal G:C {yields} A:T mutation bias in bacteria, fungi, plants and animals. To explore the molecular basis for this mutation bias, we examined the three well-known DNA mutation models, i.e., oxidative damage model, UV-radiation damage model and CpG hypermutation model. It was revealed that these models cannot provide a sufficient explanation to the universal mutation bias. Therefore, we resorted to a DNA mutation model proposed by Loewdin 40 years ago, which was based on inter-base double proton transfers (DPT). Since DPT is a fundamental and spontaneous chemical process and occurs much more frequently within GC pairs than AT pairs, Loewdin model offers a common explanation for the observed universal mutation bias and thus has broad biological implications.
Fu, Liang-Yu [National Key Laboratory of Crop Genetic Improvement, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070 (China) [National Key Laboratory of Crop Genetic Improvement, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070 (China); Center for Bioinformatics, Huazhong Agricultural University, Wuhan 430070 (China); Wang, Guang-Zhong [State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101 (China)] [State Key Laboratory of Brain and Cognitive Science, Institute of Biophysics, Chinese Academy of Sciences, Beijing 100101 (China); Ma, Bin-Guang [Center for Bioinformatics, Huazhong Agricultural University, Wuhan 430070 (China)] [Center for Bioinformatics, Huazhong Agricultural University, Wuhan 430070 (China); Zhang, Hong-Yu, E-mail: firstname.lastname@example.com [National Key Laboratory of Crop Genetic Improvement, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070 (China) [National Key Laboratory of Crop Genetic Improvement, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070 (China); Center for Bioinformatics, Huazhong Agricultural University, Wuhan 430070 (China)
M&D Professional Services, Inc. (M&D) is under subcontract to Pacific Northwest National Laboratories (PNNL) to perform seismic analysis of the Hanford Site Double-Shell Tanks (DSTs) in support of a project entitled Double-Shell Tank (DST) Integrity Project - DST Thermal and Seismic Analyses. The original scope of the project was to complete an up-to-date comprehensive analysis of record of the DST System at Hanford in support of Tri-Party Agreement Milestone M-48-14. The work described herein was performed in support of the seismic analysis of the DSTs. The thermal and operating loads analysis of the DSTs is documented in Rinker et al. (2004). Although Milestone M-48-14 has been met, Revision I is being issued to address external review comments with emphasis on changes in the modeling of anchor bolts connecting the concrete dome and the steel primary tank. The work statement provided to M&D (PNNL 2003) required that a nonlinear soil structure interaction (SSI) analysis be performed on the DSTs. The analysis is required to include the effects of sliding interfaces and fluid sloshing (fluid-structure interaction). SSI analysis has traditionally been treated by frequency domain computer codes such as SHAKE (Schnabel, et al. 1972) and SASSI (Lysmer et al. 1999a). Such frequency domain programs are limited to the analysis of linear systems. Because of the contact surfaces, the response of the DSTs to a seismic event is inherently nonlinear and consequently outside the range of applicability of the linear frequency domain programs. That is, the nonlinear response of the DSTs to seismic excitation requires the use of a time domain code. The capabilities and limitations of the commercial time domain codes ANSYS{reg_sign} and MSC Dytran{reg_sign} for performing seismic SSI analysis of the DSTs and the methodology required to perform the detailed seismic analysis of the DSTs has been addressed in Rinker et al (2006a). On the basis of the results reported in Rinker et al. (2006a), it is concluded that time-domain SSI analysis using ANSYS{reg_sign} is justified for predicting the global response of the DSTs. The most significant difference between the current revision (Revision 1) of this report and the original issue (Revision 0) is the treatment of the anchor bolts that tie the steel dome of the primary tank to the concrete tank dome.
M&D Professional Services, Inc. (M&D) is under subcontract to Pacific Northwest National Laboratory (PNNL) to perform seismic analysis of the Hanford Site double-shell tanks (DSTs) in support of a project entitled ''Double-Shell Tank (DSV Integrity Project--DST Thermal and Seismic Analyses)''. The overall scope of the project is to complete an up-to-date comprehensive analysis of record of the DST system at Hanford in support of Tri-Party Agreement Milestone M-48-14, The work described herein was performed in support of the seismic analysis of the DSTs. The thermal and operating loads analysis of the DSTs is documented in Rinker et al. (2004). The work statement provided to M&D (PNNL 2003) required that the seismic analysis of the DSTs assess the impacts of potentially non-conservative assumptions in previous analyses and account for the additional soil mass due to the as-found soil density increase, the effects of material degradation, additional thermal profiles applied to the full structure including the soil-structure response with the footings, the non-rigid (low frequency) response of the tank roof, the asymmetric seismic-induced soil loading, the structural discontinuity between the concrete tank wall and the support footing and the sloshing of the tank waste. The seismic analysis considers the interaction of the tank with the surrounding soil and the effects of the primary tank contents. The DSTs and the surrounding soil are modeled as a system of finite elements. The depth and width of the soil incorporated into the analysis model are sufficient to obtain appropriately accurate analytical results. The analyses required to support the work statement differ from previous analysis of the DSTs in that the soil-structure interaction (SSI) model includes several (nonlinear) contact surfaces in the tank structure, and the contained waste must be modeled explicitly in order to capture the fluid-structure interaction behavior between the primary tank and contained waste.
Method of quantitating dsDNA
A method for quantitating dsDNA in an aqueous sample solution containing an unknown amount of dsDNA. A first aqueous test solution containing a known amount of a fluorescent dye-dsDNA complex and at least one fluorescence-attenutating contaminant is prepared. The fluorescence intensity of the test solution is measured. The first test solution is diluted by a known amount to provide a second test solution having a known concentration of dsDNA. The fluorescence intensity of the second test solution is measured. Additional diluted test solutions are similarly prepared until a sufficiently dilute test solution having a known amount of dsDNA is prepared that has a fluorescence intensity that is not attenuated upon further dilution. The value of the maximum absorbance of this solution between 200-900 nanometers (nm), referred to herein as the threshold absorbance, is measured. A sample solution having an unknown amount of dsDNA and an absorbance identical to that of the sufficiently dilute test solution at the same chosen wavelength is prepared. Dye is then added to the sample solution to form the fluorescent dye-dsDNA-complex, after which the fluorescence intensity of the sample solution is measured and the quantity of dsDNA in the sample solution is determined. Once the threshold absorbance of a sample solution obtained from a particular environment has been determined, any similarly prepared sample solution taken from a similar environment and having the same value for the threshold absorbance can be quantified for dsDNA by adding a large excess of dye to the sample solution and measuring its fluorescence intensity.
Stark, Peter C. (Los Alamos, NM); Kuske, Cheryl R. (Los Alamos, NM); Mullen, Kenneth I. (Los Alamos, NM)
Consistency test of neutrinoless double beta decay with one isotope
We discuss a consistency test which makes it possible to discriminate unknown nuclear background lines from neutrinoless double beta decay with only one isotope. By considering both the transition to the ground state and to the first excited $0^+$ state, a sufficiently large detector can reveal if neutrinoless double beta decay or some other nuclear physics process is at work. Such a detector could therefore simultaneously provide a consistency test for a certain range of Majorana masses and be sensitive to lower values of the effective Majorana mass.
Michael Duerr; Manfred Lindner; Kai Zuber
Slug Test Data Analysis in Reservoirs with Double Porosity Behaviour
Pressure analysis for a slug test which corresponds to the flow period of a Drill Stem test is extended to wells in reservoirs with double-porosity behaviour. Solutions are obtained for either pseudo-steady state or transient interporosity flow. The distinctive specific features of both solutions are identified. Results presented are applicable to both naturally-fractured and layered reservoirs with the more permeable layer connecting to the wellbore. Type curves based on the pseudo-steady or transient interporosity flow are presented. These type curves are similar to the existing homogenous single layer type curve with addition of interporosity flow lines indicating double-porosity behaviour.
Mateen, Khalid; Ramey, Henry J. Jr.
Implementation of Double-Waist Chicane Optics in SPEAR3
The SPEAR3 accelerator upgrade opened up two 7.6m racetrack straights in the magnet lattice. In one of these straights, we recently added a magnetic chicane to separate two insertion device (ID) beam lines by 10mrad. A quadrupole triplet in the center creates a ''double focus'' optics with {beta}y = 1.6m at the middle of each ID, hence the term ''double-waist chicane''. The new optics also reduced {beta}y in the four matching straights adjacent to the racetrack straights to 2.5m. In this paper, we outline design features of the optics and physical implementation of the lattice.
Corbett, J.; Cornacchia, M.; Dao, T.; Dell'Orco, D.; Rafael, F.; Harrington, D.; Hettel, R.; Huang, X.; Nosochkov, Y.; Rabedeau, T.; Rarback, H.; Ringwall, A.; Safranek,; Scott, B.; Sebek, J.; Tanabe, J.; Terebilo, A.; Wermelskirchen, C.; Widmeyer, M.; /SLAC, SSRL; Yoon, Moohyun; /POSTECH
We investigate the possibility of producing neutrinoless double beta decay without having an electron neutrino with a mass in the vicinity of 1 eV. We do so by having a much lighter electron neutrino mix with a much heavier (m > 1 GeV) sterile neutrino. We study the constraints on the masses and mixings of such heavy sterile neutrinos from existing laboratory, astrophysical and cosmological information, and discuss the properties it would require in order to produce a detectable signal in current searches for neutrinoless double beta decay.
P. Bamert; C. P. Burgess; R. N. Mohapatra
Empirical Survey of Neutrinoless Double Beta Decay Matrix Elements
Neutrinoless double beta decay has been the subject of intensive theoretical work as it represents the only practical approach to discovering whether neutrinos are Majorana particles or not, and whether lepton number is a conserved quantum number. Available calculations of matrix elements and phase-space factors are reviewed from the perspective of a future large-scale experimental search for neutrinoless double beta decay. Somewhat unexpectedly, a uniform inverse correlation between phase space and the square of the nuclear matrix element emerges. As a consequence, no isotope is either favored or disfavored; all have qualitatively the same decay rate per unit mass for any given value of the Majorana mass.
R. G. H. Robertson
Double-{beta} decay Q value of {sup 150}Nd
The double-{beta} decay Q value of {sup 150}Nd was determined by using the JYFLTRAP Penning trap mass spectrometer. The measured mass difference between {sup 150}Nd and {sup 150}Sm is 3371.38(20) keV. This new value deviates by 3.7 keV from the previously adopted value of 3367.7(22) keV and is a factor of 10 more precise. Accurate knowledge of this Q value is important because {sup 150}Nd is a primary candidate to be used in the search for neutrinoless double-{beta} decay modes in several experiments.
Kolhinen, V. S.; Eronen, T.; Gorelov, D.; Hakala, J.; Jokinen, A.; Kankainen, A.; Moore, I. D.; Rissanen, J.; Saastamoinen, A.; Suhonen, J.; Aeystoe, J. [Department of Physics, P. O. Box 35 (YFL), FI-40014 University of Jyvaeskylae (Finland)
We discuss a consistency test which makes it possible to discriminate unknown nuclear background lines from neutrinoless double beta decay with only one isotope. By considering both the transition to the ground state and to the first excited 0{sup +} state, a sufficiently large detector can reveal if neutrinoless double beta decay or some other nuclear physics process is at work. Such a detector could therefore simultaneously provide a consistency test for a certain range of Majorana masses and be sensitive to lower values of the effective Majorana mass .
Duerr, Michael; Lindner, Manfred [Max-Planck-Institut fuer Kernphysik, Postfach 10 39 80, 69029 Heidelberg (Germany); Zuber, Kai [Technical University Dresden, Institut fuer Kern- und Teilchenphysik, 01069 Dresden (Germany)
Multiple-resonance phenomenon in neutrinoless double-electron capture
A superposition of multiple resonance states in neutrinoless double-electron capture in {sup 156}Dy has been discovered. Penning-trap mass spectrometry has been used for atomic-mass-difference measurements and careful calculations of electron wave functions and double-hole binding energies have been performed to determine the resonance-enhancement factors. Transitions to four nuclear excited states in the daughter nuclide {sup 156}Gd have been identified as resonantly enhanced, including one with a full resonant enhancement, within the uncertainty of 100 eV. This phenomenon is unique in that it can be used to probe the mechanisms of neutrinoless processes.
Eliseev, S.; Goncharov, M. [Max-Planck-Institut fuer Kernphysik, Saupfercheckweg 1, D-69117 Heidelberg (Germany); Blaum, K. [Max-Planck-Institut fuer Kernphysik, Saupfercheckweg 1, D-69117 Heidelberg (Germany); Physikalisches Institut, Ruprecht-Karls-Universitaet, D-69120 Heidelberg (Germany); Block, M.; Herfurth, F.; Minaya Ramirez, E. [GSI Helmholtzzentrum fuer Schwerionenforschung GmbH, Planckstrasse 1, D-64291 Darmstadt (Germany); Droese, C.; Schweikhard, L. [Institut fuer Physik, Ernst-Moritz-Arndt-Universitaet, D-17487 Greifswald (Germany); Novikov, Yu. N. [Max-Planck-Institut fuer Kernphysik, Saupfercheckweg 1, D-69117 Heidelberg (Germany); Petersburg Nuclear Physics Institute, Gatchina, RU-188300 St. Petersburg (Russian Federation); Department of Physics, St. Petersburg State University, RU-198504 St. Petersburg (Russian Federation); Shabaev, V. M.; Tupitsyn, I. I.; Zubova, N. A. [Department of Physics, St. Petersburg State University, RU-198504 St. Petersburg (Russian Federation); Zuber, K. [Institut fuer Kern- und Teilchenphysik, Technische Universitaet, D-01069 Dresden (Germany)
Period doubling, information entropy, and estimates for Feigenbaum's constants
The relationship between period doubling bifurcations and Feigenbaum's constants has been studied for nearly 40 years and this relationship has helped uncover many fundamental aspects of universal scaling across multiple nonlinear dynamical systems. This paper will combine information entropy with symbolic dynamics to demonstrate how period doubling can be defined using these tools alone. In addition, the technique allows us to uncover some unexpected, simple estimates for Feigenbaum's constants which relate them to log 2 and the golden ratio, phi, as well as to each other.
Reginald D. Smith
DNA fragment sizing and sorting by laser-induced fluorescence
A method is provided for sizing DNA fragments using high speed detection systems, such as flow cytometry to determine unique characteristics of DNA pieces from a sample. In one characterization the DNA piece is fragmented at preselected sites to produce a plurality of DNA fragments. The DNA piece or the resulting DNA fragments are treated with a dye effective to stain stoichiometrically the DNA piece or the DNA fragments. The fluorescence from the dye in the stained fragments is then examined to generate an output functionally related to the number of nucleotides in each one of the DNA fragments. In one embodiment, the intensity of the fluorescence emissions from each fragment is linearly related to the fragment length. The distribution of DNA fragment sizes forms a characterization of the DNA piece for use in forensic and research applications.
Hammond, Mark L. (Angier, NC); Jett, James H. (Los Alamos, NM); Keller, Richard A. (Los Alamos, NM); Marrone, Babetta L. (Los Alamos, NM); Martin, John C. (Los Alamos, NM)
Sequential addition of short DNA oligos in DNA-polymerase-based synthesis reactions
A method of preselecting a multiplicity of DNA sequence segments that will comprise the DNA molecule of user-defined sequence, separating the DNA sequence segments temporally, and combining the multiplicity of DNA sequence segments with at least one polymerase enzyme wherein the multiplicity of DNA sequence segments join to produce the DNA molecule of user-defined sequence. Sequence segments may be of length n, where n is an odd integer. In one embodiment the length of desired hybridizing overlap is specified by the user and the sequences and the protocol for combining them are guided by computational (bioinformatics) predictions. In one embodiment sequence segments are combined from multiple reading frames to span the same region of a sequence, so that multiple desired hybridizations may occur with different overlap lengths.
Gardner, Shea N; Mariella, Jr., Raymond P; Christian, Allen T; Young, Jennifer A; Clague, David S
Linear Thermodynamics of Rodlike DNA Filtration
Linear thermodynamics transportation theory is employed to study filtration of rodlike DNA molecules. Using the repeated nanoarray consisting of alternate deep and shallow regions, it is demonstrated that the complex ...
Li, Zirui
Intriguing DNA Editor Has a Structural Trigger
generating a lot of excitement because, unlike earlier genome-editing tools, its basic architecture doesn't have to be rebuilt each time a new section of DNA is targeted; it...
The Initiation of Bacterial DNA Replication
that the ATP-DnaA active site is in a closed configuration, which allows it to bind nucleotides using conserved residues from neighboring AAA+ protomers. The precise geometry of...
Deoxyribose oxidation chemistry and endogenous DNA adducts
Endogenous and exogenous oxidants react with cellular macromolecules to generate a variety of electrophiles that react with DNA produce cytotoxic and mutagenic adducts. One source of such electrophiles is deoxyribose in ...
Zhou, Xinfeng
Double Shell Tank (DST) Monitor and Control Subsystem Definition Report
The system description of the Double-Shell Tank (DST) Monitor and Control Subsystem establishes the system boundaries and describes the interface of the DST Monitor and Control Subsystem with new and existing systems that are required to accomplish the Waste Feed Delivery (WFD) mission.
Neutrinoless Double Beta Decay with R-parity Violation
We consider recently observed neutrinoless double beta decay in the context of the minimal supersymmetric standard model with R-parity violating couplings lambda^{'}. We observe that most of the current experimental bounds on the R-parity violating couplings do not exclude the possibility that the neutrinoless double beta decay is caused by R-parity violation. But if we consider K-bar{K} oscillation, we observe that we have to make the R-parity violating couplings generation-dependent to accomodate with the observed neutrinoless double beta decay. And furthermore, we need some mechanism to cancel the contribution to K-bar{K} mixing from a large R-parity violating coupling. We realized this cancellation by assuming that the first- and the second- generation of quark sector do not couple with the first-generation lepton sector by R-parity violating couplings except the term W=lambda_{111}^{'} L_{1} Q_{1} D_{1}^{c}, which is responsible for the observed neutrinoless double beta decay.
Yosuke Uehara
Neutrinoless double beta decay mediated by the neutrino magnetic moment
We present a new channel of the neutrinoless double beta decay. In this scenario neutrinos not only oscillate inside the nucleus but also interact with an external non-uniform magnetic field. We assume that the field rotates about the direction of motion of the neutrino and show, that for a certain speed of rotation the half-life of the $0\
Marek G�?d?; Wies?aw A. Kami?ski
Conditions for detecting CP violation via neutrinoless double beta decay
Neutrinoless double beta decay data together with information on the absolute neutrino masses obtained from the future KATRIN experiment and/or astrophysical measurements give a chance to find CP violation in the lepton sector with Majorana neutrinos. We derive and discuss necessary conditions which make discovery of such CP violation possible for the future neutrino oscillation and mass measurements data.
Alicja Joniec; Marek Zralek
EA-1905: Double Eagle Water System, Carlsbad, New Mexico
This EA, prepared by the U.S. Department of the Interior�s Bureau of Land Management Carlsbad Field Office and adopted by DOE, evaluates the expansion and upgrade of the City of Carlsbad�s Double Eagle Water System.
Pd/Ni-WO3 anodic double layer gasochromic device
An anodic double layer gasochromic sensor structure for optical detection of hydrogen in improved response time and with improved optical absorption real time constants, comprising: a glass substrate; a tungsten-doped nickel oxide layer coated on the glass substrate; and a palladium layer coated on the tungsten-doped nickel oxide layer.
Lee, Se-Hee; Tracy, C. Edwin; Pitts, J. Roland; Liu, Ping
The Double Charm Baryon Family at SELEX James S. Russ
-Charm Baryon Spectroscopy SELEX Double Charm Wine and Cheese 6/13/03. 1 #12;The SELEX Collaboration G.P. Thomas.A. Victorov Institute for High Energy Physics, Protvino, Russia Li Yunshan, Mao Chensheng, Zhao Wenheng, He Kangling, Zheng Shuchen, Mao Zhenlin Institute of High Energy Physics, Beijing, P.R. China M.Y. Balatz5, G
Fermi National Accelerator Laboratory
NON-DOUBLE-COUPLE EARTHQUAKES: NET FORCES AND UNCERTAINTIES G.R. Foulger, B.R. Julian University-980) to include net forces in the mechanisms. Net forces are theoretically required to describe earthquakes) waves cannot resolve sources such as vertical dipoles. When source mechanisms include net forces, even
Foulger, G. R.
Sound radiation of double reed woodwinds F. B. Konkel1
Sound radiation of double reed woodwinds F. B. Konkel1 , A. Jakob1 , F. Heintze2 , M. M¨oser1 1 position for the active influence [1]. Sound radiation is the ratio between the sound pressure radiated by the instrument to the sound pressure inside the instrument. Hence, one hundred percent sound radiation is a full
Berlin,Technische Universität
Double beta decay to the excited states: experimental review
A brief review on double beta decay to excited states of daughter nuclei is given. The ECEC(ov) transision to the excited states are discussed in association with a possible enhancement of the decay rate by several orders of magnitude.
Generator Coordinate Method framework for Double Beta Decay
We propose a consistent prescription for the derivation of the particle number and angular momentum projected QRPA (PQRPA) equation in the Generator Coordinate Method (GCM) framework for calculation of NME's of double-beta decay of axially deformed nuclei. We derive closed formulae for the calculation of excitation energies and wave functions of the intermediate nucleus.
Andrzej Bobyk; Wies?aw A. Kami?ski
An increased estimate of the merger rate of double neutron
-star merger rate in the Galaxy is crucial in order to predict whether current gravity wave detectors of only a few double-neutron-star binaries with merger times less than the age of the Universe. Here we position and flux density for the pulsar. Knowledge of the pulsar position with subarcsecond precision
Sarkissian, John M.
Double Shell Tank AY-102 Radioactive Waste Leak Investigation
PowerPoint. The objectives of this presentation are to: Describe Effort to Determine Whether Tank AY-102 Leaked; Review Probable Causes of the Tank AY-102 Leak; and, Discuss Influence of Leak on Hanford�s Double-Shell Tank Integrity Program.
Washenfelder, Dennis J.
Polymerization dynamics of double-stranded biopolymers: Chemical kinetic approach
Polymerization dynamics of double-stranded biopolymers: Chemical kinetic approach Evgeny B 19 November 2004; accepted 20 December 2004; published online 10 March 2005 The polymerization by the processes that take place during their polymerization. However, our understanding of the coupling between
Geometrical tunability of plasmon excitations of double concentric metallic nanotubes
The plasmon frequencies of a general double concentric metallic nanotube (NT) are obtained by using the plasmon hybridization method. Theoretical calculations indicate that there are four distinct plasmon modes for the system. It is shown that these two alternating layers of dielectric and metal have a greater geometrical tunability than the single metallic nanotubes of similar dimensions.
Moradi, Afshin [Department of Nano Science, Kermanshah University of Technology, Kermanshah 67178-63766 (Iran, Islamic Republic of) and Department of Nano Science, Institute for Studies in Theoretical Physics and Mathematics (IPM), Tehran 19395-5531 (Iran, Islamic Republic of)
Scaling Factor Inconsistencies in Neutrinoless Double Beta Decay
The modern theory of neutrinoless double beta decay includes a scaling factor that has often been treated inconsistently in the literature. The nuclear contribution to the decay half life can be suppressed by 15-20% when scaling factors are mismatched. Correspondingly, $$ is overestimated.
S. Cowell
Scaling factor inconsistencies in neutrinoless double beta decay
The modern theory of neutrinoless double beta decay includes a scaling factor that has often been treated inconsistently in the literature. The nuclear contribution to the decay half-life can be suppressed by 15%-20% when scaling factors are mismatched. Correspondingly, is overestimated.
Cowell, S. [Theoretical Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545 (United States)
New Advances in Neutrinoless Double Beta Decay Matrix Elements
We present the matrix elements necessary to evaluate the half-life of some neutrinoless double beta decay candidates in the framework of the microscopic interacting boson model (IBM). We compare our results with those from other models and extract some simple features of the calculations.
Munoz, Jose Barea [Instituto de Estructura de la Materia, C.S.I.C. Unidad Asociada al Departamento de Fisica Atomica, Molecular y Nuclear, Facultad de Fisica, Universidad de Sevilla, Apartado 1065, 41080 Sevilla (Spain)
Neutrinoless Double Beta Decay and {nu}-Mass Determination
The search for Neutrinoless Double Beta Decay could improve our knowledge on neutrino properties. After a brief discussion on the implications of the observation of this rare process, I will introduce the experimental approaches and review the prospects of the search for this nuclear transition.
Pedretti, M. [Universita dell Insubria, Via Vallegio 11, 22100 Como (Italy)
Neutrinoless double beta decay in the microscopic interacting boson model
The results of a calculation of the nuclear matrix elements for neutrinoless double beta decay in the closure approximation in several nuclei within the framework of the microscopic interacting boson model (IBM-2) are presented and compared with those calculated in the shell model (SM) and quasiparticle random phase approximation (QRPA)
Iachello, F. [Center for Theoretical Physics, Sloane Physics Laboratory Yale University New Haven, CT 06520-8120 (United States)
Majorana neutrino masses and the neutrinoless double-beta decay
Neutrinoless double-beta decay is forbidden in the Standard Model of electroweak and strong interaction but allowed in most Grand Unified Theories (GUTs). Only if the neutrino is a Majorana particle (identical with its antiparticle) and if it has a mass is neutrinoless double-beta decay allowed. Apart from one claim that the neutrinoless double-beta decay in {sup 76}Ge is measured, one has only upper limits for this transition probability. But even the upper limits allow one to give upper limits for the electron Majorana neutrino mass and upper limits for parameters of GUTs and the minimal R-parity-violating supersymmetric model. One further can give lower limits for the vector boson mediating mainly the right-handed weak interaction and the heavy mainly right-handed Majorana neutrino in left-right symmetric GUTs. For that, one has to assume that the specific mechanism is the leading one for neutrinoless double-beta decay and one has to be able to calculate reliably the corresponding nuclear matrix elements. In the present work, one discusses the accuracy of the present status of calculating of the nuclear matrix elements and the corresponding limits of GUTs and supersymmetric parameters.
Faessler, A. [University of Tuebingen, Institute of Theoretical Physics (Germany)], E-mail: email@example.com
Neutrinoless double-{beta} decay: Status and future
A brief summary of the status of neutrino masses, mixing, and oscillations is presented. Neutrinoless double {beta} decay is considered. Predictions for the effective Majorana mass are reviewed. A possible test of the calculations of nuclear matrix elements of the 0{nu}{beta}{beta} decay is proposed.
Bilenky, S. M. [Joint Institute for Nuclear Research (Russian Federation)], E-mail: email@example.com
Neutrinoless double beta decay data, together with information on the absolute neutrino masses obtained from the future KATRIN experiment and/or astrophysical measurements, provide a chance to find CP violation in the lepton sector with Majorana neutrinos. We derive and discuss necessary conditions which make discovery of such CP violation possible for the future neutrino oscillation and mass measurements data.
Joniec, A.; Zralek, M. [Institute of Physics, University of Silesia, Uniwersytecka 4, 40-007 Katowice (Poland)
G�?d?, Marek
TRIBAND BRANCH LINE COUPLER USING DOUBLE-LORENTZ TRANSMISSION LINES Hanseung Lee and Sangwook Nam) transmission lines (TL) provides two additional degrees of freedom in realizing triband microwave devices-Lorentz; transmission lines; triband; directional couplers 1. INTRODUCTION The concept of artificial TLs having
Nam, Sangwook
Neutrinoless double-beta decay and seesaw mechanism
From the standard seesaw mechanism of neutrino mass generation, which is based on the assumption that the lepton number is violated at a large (~10exp(+15) GeV) scale, follows that the neutrinoless double-beta decay is ruled by the Majorana neutrino mass mechanism. Within this notion, for the inverted neutrino-mass hierarchy we derive allowed ranges of half-lives of the neutrinoless double-beta decay for nuclei of experimental interest with different sets of nuclear matrix elements. The present-day results of the calculation of the neutrinoless double-beta decay nuclear matrix elements are briefly discussed. We argue that if neutrinoless double-beta decay will be observed in future experiments sensitive to the effective Majorana mass in the inverted mass hierarchy region, a comparison of the derived ranges with measured half-lives will allow us to probe the standard seesaw mechanism assuming that future cosmological data will establish the sum of neutrino masses to be about 0.2 eV.
Samoil M. Bilenky; Amand Faessler; Walter Potzel; Fedor Simkovic
Double-Sided Cooling Design for Novel Planar Module
A novel packaging structure for medium power modules featuring power semiconductor switches sandwiched between two symmetric substrates that fulfill electrical conduction and insulation functions is presented. Large bonding areas between dies and substrates allow this packaging technology to offer significant improvements in electrical, thermal performance. Double-sided cooling system was dedicatedly analyzed and designed for different applications.
Ning, Puqi [ORNL; Liang, Zhenxian [ORNL; Marlino, Laura D [ORNL; Wang, Fei [ORNL
Circuit QED in a double quantum dot system
Strong coupling peculiar feature is demonstrated in a coupled qubit-resonator system consisting of a GaAs double quantum dot and a coplanar waveguide resonator. Qubit-resonator coupling strength (g and the decoherence rate ? are directly derived from the experiment, assuring a strong coupling condition (g/? ? 2)
Toida, Hiraku; Nakajima, Takashi; Komiyama, Susumu [Department of Basic Science, University of Tokyo, 3-8-1 Komaba, Meguro-ku, Tokyo 153-8902 (Japan)
Illustrative Model for Parity Doubling of Energy Levels
A one-dimensional quantum mechanical model possessing mass gap, a gapless excitation, and an approximate parity doubling of energy levels is constructed basing on heuristic QCD-inspired arguments. The model may serve for illustrative purposes in considering the related dynamical phenomena in particle and nuclear physics.
S. S. Afonin
Double Beta Decay and the Absolute Neutrino Mass Scale
After a short review of the current status of three-neutrino mixing, the implications for the values of neutrino masses are discussed. The bounds on the absolute scale of neutrino masses from Tritium beta-decay and cosmological data are reviewed. Finally, we discuss the implications of three-neutrino mixing for neutrinoless double-beta decay.
Carlo Giunti
Effects of New Gravitational Interactions on Neutrinoless Double Beta Decay
It has recently been proposed that violations of Lorentz invariance or violations of the equivalence principle can be constrained from the non-observation of neutrinoless double beta decay. We generalize this analysis to all possible new gravitational interactions and discuss briefly the constraints for different cases.
H. V. Klapdor--Kleingrothaus; H. P�s; U. Sarkar
Double Passive Cavitation Detection of OptisonTM Shell Rupture
Double Passive Cavitation Detection of OptisonTM Shell Rupture Azzdine Y. Ammi1 , Robin O). The experimental setup is based on a passive cavitation detection system described in previous work. However by ultrasonic capsule destruction [3,4]. In previous work using a passive cavitation detection (PCD) system [5
Illinois at Urbana-Champaign, University of
A Calorimetric Search on Double Beta Decay of 130Te
We report on the final results of a series of experiments on double decay of 130Te carried out with an array of twenty cryogenic detectors. The set-up is made with crystals of TeO2 with a total mass of 6.8 kg, the largest operating one for a cryogenic experiment. Four crystals are made with isotopically enriched materials: two in 128Te and two others in 130Te. The remaining ones are made with natural tellurium, which contains 31.7 % and 33.8 % 128Te and 130Te, respectively. The array was run under a heavy shield in the Gran Sasso Underground Laboratory at a depth of about 3500 m.w.e. By recording the pulses of each detector in anticoincidence with the others a lower limit of 2.1E23 years has been obtained at the 90 % C.L. on the lifetime for neutrinoless double beta decay of 130Te. In terms of effective neutrino mass this is the most restrictive limit in direct experiments, after those obtained with Ge diodes. Limits on other lepton violating decays of 130Te and on the neutrinoless double beta decay of 128Te to the ground state of 128Xe are also reported and discussed. An indication is presented for the two neutrino double beta decay of 130Te. Some consequences of the present results in the interpretation of geochemical experiments are discussed.
C. Arnaboldi; C. Brofferio; C. Bucci; S. Capelli; O. Cremonesi; E. Fiorini; A. Giuliani; A. Nucciotti; M. Pavan; M. Pedretti; G. Pessina; S. Pirro; C. Pobes; E. Previtali; M. Sisti; M. Vanzini
Research Note Microsatellite Variation of Double-Crested Cormorant
Environmental Impact Statement on double- crested cormorant management allowing more flexibility in control-crested cormorants roosting in the lower Mis- sissippi Valley appear to have minimal negative impact on sport fisheries, they may have a significant impact on commercial aquaculture production in this region (Glahn
Green, Clay - Department of Biology, Texas State University
Comparing Control Constructs by Double-barrelled CPS Hayo Thielecke
Comparing Control Constructs by Double-barrelled CPS Hayo Thielecke (h classical in the source of the CPS transform break the linearity of continuation use in the target. Keywords operators to the bare essentials of labelling and jumping, so that there are no longer any distracting
Ultrasonic Examination of Double-Shell Tank 214-AN-101
COEGMA Engineering Corporation (COGEMA) under a contract from CH2M Hill Hanford Group (CH2M Hill) has performed an ultrasonic examination of selected portions of Double-Shell Tank 241-AN-101. PNNL is responsible for preparing a report(s) that describes the results of the COGEMs ultrasonic examinations.
Pardini, Allan F.; Posakony, Gerald J.
RecA acts in trans to allow replication of damaged DNA by DNA polymerase V
, Michael M. Cox2 , Roger Woodgate3 & Myron F. Goodman1 The DNA polymerase V (pol V) and RecA proteins
Design of Autonomous DNA Cellular Automata , Sudheer Sahu
Design of Autonomous DNA Cellular Automata Peng Yin ¡ , Sudheer Sahu ¡ , Andrew J. Turberfield cellular computing devices, i.e. autonomous nano-mechanical DNA computing devices embedded in DNA lattices. As a prototype of such devices, we recently reported the design of an Autonomous DNA Turing Machine, which
Recombinant DNA Research in Human Subjects Duke Institutional Biosafety Committee
/2010 Summary. All Duke research using recombinant DNA (rDNA) in human subjects must comply with the NIH Guidelines for Research involving Recombinant DNA Molecules (available at http://oba.od.nih.gov/rdna/nih Biosafety Committee (IBC) [NIH Guidelines Section III-C and III-C-1]. The NIH defines rDNA broadly
Double Macdonald polynomials as the stable limit of Macdonald superpolynomials
Macdonald superpolynomials provide a remarkably rich generalization of the usual Macdonald polynomials. The starting point of this work is the observation of a previously unnoticed stability property of the Macdonald superpolynomials when the fermionic sector m is sufficiently large: their decomposition in the monomial basis is then independent of m. These stable superpolynomials are readily mapped into bisymmetric polynomials, an operation that spoils the ring structure but drastically simplifies the associated vector space. Our main result is a factorization of the (stable) bisymmetric Macdonald polynomials, called double Macdonald polynomials and indexed by pairs of partitions, into a product of Macdonald polynomials (albeit subject to non-trivial plethystic transformations). As an off-shoot, we note that, after multiplication by a t-Vandermonde determinant, this provides explicit formulas for a large class of Macdonald polynomials with prescribed symmetry. The factorization of the double Macdonald polynomials leads immediately to the generalization of basically every elementary properties of the Macdonald polynomials to the double case (norm, kernel, duality, positivity, etc). When lifted back to superspace, this validates various previously formulated conjectures in the stable regime. The q,t-Kostka coefficients associated to the double Macdonald polynomials are shown to be q,t-analogs of the dimensions of the irreducible representations of the hyperoctahedral group B_n. Moreover, a Nabla operator on the double Macdonald polynomials is defined and its action on a certain bisymmetric Schur function can be interpreted as the Frobenius series of a bigraded module of dimension (2n+1)^n, a formula again characteristic of the Coxeter group of type B_n. Finally, as a side result, we obtain a simple identity involving products of four Littlewood-Richardson coefficients.
O. Blondeau-Fournier; L. Lapointe; P. Mathieu
Industrial mixing techniques for Hanford double-shell tanks
Jet mixer pumps are currently the baseline technology for sludge mobilization and mixing in one-million gallon double-shell tanks at the Hanford and Savannah River Sites. Improvements to the baseline jet mixer pump technology are sought because jet mixer pumps have moving parts that may fail or require maintenance. Moreover, jet mixers are relatively expensive, they heat the waste, and, in some cases, may not mobilize enough of the sludge. This report documents a thorough literature search for commercially available applicable mixing technologies that could be used for double-shell tank sludge mobilization and mixing. Textbooks, research articles, conference proceedings, mixing experts, and the Thomas Register were consulted to identify applicable technologies. While there are many commercial methods that could be used to mobilize sludge or mix the contents of a one-million gallon tank, few will work given the geometrical constraints (e.g., the mixer must fit through a 1.07-m-diameter riser) or the tank waste properties (e.g., the sludge has such a high yield stress that it generally does not flow under its own weight). Pulsed fluid jets and submersible Flygt mixers have already been identified at Hanford and Savannah River Sites for double-shell tank mixing applications. While these mixing technologies may not be applicable for double-shell tanks that have a thick sludge layer at the bottom (since too many of these mixers would need to be installed to mobilize most of the sludge), they may have applications in tanks that do not have a settled solids layer. Retrieval projects at Hanford and other U.S. Department of Energy sites are currently evaluating the effectiveness of these mixing techniques for tank waste applications. The literature search did not reveal any previously unknown technologies that should be considered for sludge mobilization and mixing in one-million gallon double-shell tanks.
Daymo, E.A.
The MAJORANA DEMONSTRATOR A search for neutrinoless double-beta decay of germanium-76
MAJORANA The MAJORANA DEMONSTRATOR A search for neutrinoless double-beta decay of germanium-76 DEMONSTRATOR PANIC11 Neutrinoless double-beta decay (0) � Observation indicates: � Neutrino is a Majorana
E-Print Network 3.0 - allowed neutrinoless double Sample Search...
neutrinoless double Page: << < 1 2 3 4 5 > >> 1 What can we learn from neutrinoless double beta decay experiments? John N. Bahcall,* Hitoshi Murayama, + and C. Pen aGaray ...
NEUTRINOLESS DOUBLE-DECAY Revised August 2011 by P. Vogel (Caltech) and A. Piepke
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NEUTRINOLESS DOUBLE-DECAY Revised August 2009 by P. Vogel (Caltech) and A. Piepke
1 NEUTRINOLESS DOUBLE- DECAY Revised August 2009 by P. Vogel (Caltech) and A. Piepke (University of Alabama). Neutrinoless double-beta (0) decay would signal vio- lation of total lepton number conservation
E-Print Network 3.0 - amplitude double layers Sample Search Results
Abstract --This paper deals a double layer and a single layer Flux-Switching Permanent Magnet (FSPM) motors for a fault Summary: Abstract --This paper deals a double layer and a...
Neutrinoless double-$?$ decay of ${}^{82}$Se in the shell model: beyond closure approximation
We recently proposed a new method to calculate the standard nuclear matrix elements for neutrinoless double-$\\beta$ decay ($0\
R. A. Sen'kov; M. Horoi; B. A. Brown
A measurement of the 2 neutrino double beta decay rate of 130Te in the CUORICINO experiment
1.2.2 Neutrinoless double beta decay . . . . . 1.2.3 NuclearEvidence for Neutrinoless Double Beta Decay, Mod. Phys.on �Evidence for Neutrinoless Double Beta Decay�, Mod. Phys.
Kogler, Laura Katherine
A measurement of the 2 neutrino double beta decay rate of Te-130 in the CUORICINO experiment
Kogler, Laura
A double-blind, randomized, multicenter phase 2 study of prasugrel versus placebo in adult patients with sickle cell disease
Clinical and biological double-blind-study of ticlopidine inRESEARCH Open Access A double-blind, randomized, multicenterwe conducted a double-blind, randomized, placebo-controlled
A Double-Blind, Placebo-Controlled Trial of Oral Human Immunoglobulin for Gastrointestinal Dysfunction in Children with Autistic Disorder
y ORIGINAL PAPER A Double-Blind, Placebo-Controlled Trial ofautism. A randomized, double-blind, pla- cebo-controlled,results, a double- blind, placebo-controlled trial was
Electron spectroscopy study of single and double multiphoton ionization of strontium by visible picosecond laser light
795 Electron spectroscopy study of single and double multiphoton ionization of strontium by visible'ionisation multiphotonique simple et double du strontium par des impulsions picosecondes de 1011 à quelque 1012 W cm-2 initial un état excité de l'ion. Abstract. 2014 Multiphoton single and double ionization of strontium
Paris-Sud XI, Université de
The Free Energy of an Electrical Double Layer DEREK Y. C. CHAN AND D. JOHN MITCHELL
The Free Energy of an Electrical Double Layer DEREK Y. C. CHAN AND D. JOHN MITCHELL Department for the potential determining ions. The interaction free energy due to the overlap of two double layers has a simple The concept of the free energy of an elec- trical double layer is of considerable impor- tance in colloid
Chan, Derek Y C
ORIGINAL ARTICLE Development of a mobile welding robot for double-hull structures
ORIGINAL ARTICLE Development of a mobile welding robot for double-hull structures in shipbuilding describes the development of a self- driving mobile welding robot. The robot is used to weld U-shaped welding areas in enclosed double-hull structures. In order to place itself inside the double
Kim, Jongwon
Double beta decay, Majorana neutrinos, and neutrino mass Frank T. Avignone III*
The theoretical and experimental issues relevant to neutrinoless double beta decay are reviewed. The impact more sensitive, even the nonobservation of neutrinoless double beta decay will be useful Acknowledgments 513 References 514 I. INTRODUCTION Neutrinoless double beta decay 0 is a very slow lepton
Engel, Jonathan
1 SEARCH FOR NEUTRINOLESS DOUBLE BETA DECAY WITH THE NEMO-3 DETECTOR : FIRST RESULTS D. LALANNE to searching for neutrinoless double beta decay (0). The expected performance of the detector has been, no evidence for neutrinoless double beta decay (0) was found from ~7 kg of 100 Mo and ~1 kg of 82 Se
Boyer, Edmond
1 IS THE NON OBSERVATION OF NEUTRINOLESS DOUBLE BETA DECAY A QUESTION OF SENSITIVITY? TSAN UNG CHAN Grenoble Cedex FRANCE The hypothetical neutrinoless double beta decay is possible only if the neutrino. This contradiction would lead to conclude that neutrinoless double beta decay could not take place in nature
What can we learn from neutrinoless double beta decay experiments? John N. Bahcall,* Hitoshi Murayama,
What can we learn from neutrinoless double beta decay experiments? John N. Bahcall,* Hitoshi-generation neutrinoless double beta decay and normal neutrino beta decay experi- ments can answer four fundamental questions. 1 If neutrinoless double beta decay searches do not detect a signal, and if the spectrum is known
Bahcall, John
Uncertainties in Nuclear Matrix Elements for Neutrinoless Double-Beta Decay
Uncertainties in Nuclear Matrix Elements for Neutrinoless Double-Beta Decay Jonathan Engel Abstract. I briefly review calculations of the matrix elements governing neutrinoless double-beta decay, in broad terms, strategies for reducing systematic error. 1. Introduction Neutrinoless double-beta (0
Alpha Backgrounds and Their Implications for Neutrinoless Double-Beta Decay Experiments Using HPGe Detectors
Alpha Backgrounds and Their Implications for Neutrinoless Double-Beta Decay Experiments Using HPGe and Their Implications for Neutrinoless Double-Beta Decay Experiments Using HPGe Detectors Robert A. Johnson Chair of the Supervisory Committee: Professor John F. Wilkerson Physics The observation of neutrinoless double-beta decay
Experiments with Bose-Einstein Condensates in a Double-Well Potential
Experiments with Bose-Einstein Condensates in a Double-Well Potential by Yong-Il Shin B.S., Physics-Einstein Condensates in a Double-Well Potential by Yong-Il Shin Submitted to the Department of Physics on November 17 Trapped atom interferometry was demonstrated with Bose-Einstein condensates in an opti- cal double
Design of Double Salient Interior Permanent Magnet Machine Based on Mutually Coupled
Design of Double Salient Interior Permanent Magnet Machine Based on Mutually Coupled Reluctance--This paper presents a novel structure of double salient interior permanent magnet machine (DSIPM machine-- double salient, d-axis and q-axis inductances, cogging torque, permanent magnets, flux
Optical selection and collection of DNA fragments
Optical selection and collection of DNA fragments. The present invention includes the optical selection and collection of large (>.mu.g) quantities of clonable, chromosome-specific DNA from a sample of chromosomes. Chromosome selection is based on selective, irreversible photoinactivation of unwanted chromosomal DNA. Although more general procedures may be envisioned, the invention is demonstrated by processing chromosomes in a conventional flow cytometry apparatus, but where no droplets are generated. All chromosomes in the sample are first stained with at least one fluorescent analytic dye and bonded to a photochemically active species which can render chromosomal DNA unclonable if activated. After passing through analyzing light beam(s), unwanted chromosomes are irradiated using light which is absorbed by the photochemically active species, thereby causing photoinactivation. As desired chromosomes pass this photoinactivation point, the inactivating light source is deflected by an optical modulator; hence, desired chromosomes are not photoinactivated and remain clonable. The selection and photoinactivation processes take place on a microsecond timescale. By eliminating droplet formation, chromosome selection rates 50 times greater than those possible with conventional chromosome sorters may be obtained. Thus, usable quantities of clonable DNA from any source thereof may be collected.
Roslaniec, Mary C. (Los Alamos, NM); Martin, John C. (Los Alamos, NM); Jett, James H. (Los Alamos, NM); Cram, L. Scott (Los Alamos, NM)
DNA nanotechnology: understanding and optimisation through simulation
DNA nanotechnology promises to provide controllable self-assembly on the nanoscale, allowing for the design of static structures, dynamic machines and computational architectures. In this article I review the state-of-the art of DNA nanotechnology, highlighting the need for a more detailed understanding of the key processes, both in terms of theoretical modelling and experimental characterisation. I then consider coarse-grained models of DNA, mesoscale descriptions that have the potential to provide great insight into the operation of DNA nanotechnology if they are well designed. In particular, I discuss a number of nanotechnological systems that have been studied with oxDNA, a recently developed coarse-grained model, highlighting the subtle interplay of kinetic, thermodynamic and mechanical factors that can determine behaviour. Finally, new results highlighting the importance of mechanical tension in the operation of a two-footed walker are presented, demonstrating that recovery from an unintended `overstepped' configuration can be accelerated by three to four orders of magnitude by application of a moderate tension to the walker's track. More generally, the walker illustrates the possibility of biasing strand-displacement processes to affect the overall rate.
Thomas E. Ouldridge
Double Shell Tank (DST) Transfer Piping Subsystem Specification
This specification establishes the performance requirements and provides references to the requisite codes and standards to be applied during design of the Double-Shell Tank (DST) Transfer Piping Subsystem that supports the first phase of Waste Feed Delivery. This specification establishes the performance requirements and provides references to the requisite codes and standards to be applied during design of the Double-Shell Tank (DST) Transfer Piping Subsystem that supports the first phase of waste feed delivery. This subsystem transfers waste between transfer-associated structures (pits) and to the River Protection Project (RPP) Privatization Contractor Facility where it will be processed into an immobilized waste form. This specification is intended to be the basis for new projects/installations (W-521, etc.). This specification is not intended to retroactively affect previously established project design criteria without specific direction by the program.
Design of double passive modulated mode-locked lasers
A set of equations is obtained that allow the determination of the optimum values for the laser parameters in order to obtain perfect mode-locking by double passive modulation. The double modulation consists of a fast saturable absorber and a passive loss proportional to the mean intensity in the cavity provided by a passively driven electro-optic modulator. The stochastic nature of the emission of conventional passive modulated systems is eliminated if the correct values of the modulation parameters are chosen as predicted by the equations presented here. The predictions given by the equations were compared with previous numerical computations in order to check the validity of certain approximations used. Experimental results obtained confirm the predictions given by said equations.
Martinez, O.E.
Macdonald superpolynomials provide a remarkably rich generalization of the usual Macdonald polynomials. The starting point of this work is the observation of a previously unnoticed stability property of the Macdonald superpolynomials when the fermionic sector m is sufficiently large: their decomposition in the monomial basis is then independent of m. These stable superpolynomials are readily mapped into bisymmetric polynomials, an operation that spoils the ring structure but drastically simplifies the associated vector space. Our main result is a factorization of the (stable) bisymmetric Macdonald polynomials, called double Macdonald polynomials and indexed by pairs of partitions, into a product of Macdonald polynomials (albeit subject to non-trivial plethystic transformations). As an off-shoot, we note that, after multiplication by a t-Vandermonde determinant, this provides explicit formulas for a large class of Macdonald polynomials with prescribed symmetry. The factorization of the double Macdonald polynom...
Blondeau-Fournier, O; Mathieu, P
Double Beta Decay, Majorana Neutrinos, and Neutrino Mass
The theoretical and experimental issues relevant to neutrinoless double-beta decay are reviewed. The impact that a direct observation of this exotic process would have on elementary particle physics, nuclear physics, astrophysics and cosmology is profound. Now that neutrinos are known to have mass and experiments are becoming more sensitive, even the non-observation of neutrinoless double-beta decay will be useful. If the process is actually observed, we will immediately learn much about the neutrino. The status and discovery potential of proposed experiments are reviewed in this context, with significant emphasis on proposals favored by recent panel reviews. The importance of and challenges in the calculation of nuclear matrix elements that govern the decay are considered in detail. The increasing sensitivity of experiments and improvements in nuclear theory make the future exciting for this field at the interface of nuclear and particle physics.
Frank T. Avignone III; Steven R. Elliott; Jonathan Engel
Complementarity of Neutrinoless Double Beta Decay and Cosmology
Neutrinoless double beta decay experiments constrain one combination of neutrino parameters, while cosmic surveys constrain another. This complementarity opens up an exciting range of possibilities. If neutrinos are Majorana particles, and the neutrino masses follow an inverted hierarchy, then the upcoming sets of both experiments will detect signals. The combined constraints will pin down not only the neutrino masses but also constrain one of the Majorana phases. If the hierarchy is normal, then a beta decay detection with the upcoming generation of experiments is unlikely, but cosmic surveys could constrain the sum of the masses to be relatively heavy, thereby producing a lower bound for the neutrinoless double beta decay rate, and therefore an argument for a next generation beta decay experiment. In this case as well, a combination of the phases will be constrained.
Scott Dodelson; Joseph Lykken
Neutrinoless Double Beta Decay in Heavy Deformed Nuclei
The zero neutrino mode of the double beta decay in heavy deformed nuclei is investigated in the framework of the pseudo SU(3) model, which has provided an accurate description of collective nuclear structure and predicted half-lives for the two neutrino mode in good agreement with experiments. In the case of $^{238}U$ the calculated zero neutrino half-life is at least three orders of magnitude greater than the two neutrino one, giving strong support of the identification of the radiochemically determined half-life as being the two neutrino double beta decay. For $^{150}Nd$ the zero neutrino matrix elements are of the order of magnitude of, but lesser than, those evaluated using the QRPA. This result confirms that different nuclear models produce similar zero neutrino matrix elements, contrary to the two neutrino case. Using these pseudo SU(3) results and the upper limit for the neutrino mass we estimate the $\\beta\\beta_{0\
Jorge G. Hirsch; O. Casta�os; P. O. Hess
The Search for Neutrinoless Double Beta Decay in CUORE
Understanding the nature of neutrino masses will require physics beyond the long-standing Standard Model of particle physics. Neutrinoless double beta decay experiments like the Cryogenic Underground Observatory for Rare Events (CUORE) are uniquely suited for probing the remaining mysteries of neutrino mass, particularly the question of the neutrino's Majorana nature. CUORE will be a next-generation experiment at the Laboratori Nazionali del Gran Sasso in Italy; it will consist of an array of 988 TeO2 detector crystals operated at ~10 mK, following the bolometric technique established by the Cuoricino experiment. It will look for the energy signal produced by the theoretically-predicted neutrinoless double beta decay in Te-130, and therefore reliable energy calibration of the detector is crucial to the experiment's success. We will present the most recent results from Cuoricino and discuss the current status of the CUORE project, with a particular emphasis on the development of the calibration system.
L. M. Ejzak; for the CUORE Collaboration
Dodelson, Scott
Extra dimensions and neutrinoless double beta decay experiments
The neutrinoless double beta decay is one of the few phenomena, belonging to the nonstandard physics, which is extensively being sought for in experiments. In the present paper the link between the half-life of the neutrinoless double beta decay and theories with large extra dimensions is explored. The use of the sensitivities of currently planned 0{nu}2{beta} experiments: DAMA, CANDLES, COBRA, DCBA, CAMEO, GENIUS, GEM, MAJORANA, MOON, CUORE, EXO, and XMASS, gives the possibility for a nondirect 'experimental' verification of various extra dimensional scenarios. We discuss also the results of the Heidelberg-Moscow Collaboration. The calculations are based on the Majorana neutrino mass generation mechanism in the Arkani-Hamed-Dimopoulos-Dvali model.
Gozdz, Marek; Kaminski, Wieslaw A.; Faessler, Amand [Theoretical Physics Department, Maria Curie-Sklodowska University, Lublin (Poland); Institute fuer Theoretische Physik, Universitaet Tuebingen, Auf der Morgenstelle 14, D-72076 Tuebingen (Germany)
Results of the double beta decay experiment NEMO-3
The double beta decay experiment NEMO-3 has taken data from February 2003 to January 2011. The two-neutrino decay half lives were measured for seven different isotopes ({sup 100}Mo, {sup 82}Se, {sup 116}Cd, {sup 150}Nd, {sup 96}Zr, {sup 48}Ca and {sup 130}Te). No evidence for neutrinoless double beta decay is observed. The 0??? half-life limits are found to be T{sub 1/2}{sup 0?}({sup 100}Mo)>1.0�10{sup 24}yr(90%C.L.) and T{sub 1/2}{sup 0?}({sup 82}Se)>3.2�10{sup 23}yr(90%C.L.)
Tretyak, V. I. [Joint Institute for Nuclear Research, 6 Joliot Curie, Dubna (Russian Federation); Collaboration: NEMO-3 Collaboration
Double beta decay, Majorana neutrinos, and neutrino mass
The theoretical and experimental issues relevant to neutrinoless double beta decay are reviewed. The impact that a direct observation of this exotic process would have on elementary particle physics, nuclear physics, astrophysics, and cosmology is profound. Now that neutrinos are known to have mass and experiments are becoming more sensitive, even the nonobservation of neutrinoless double beta decay will be useful. If the process is actually observed, we will immediately learn much about the neutrino. The status and discovery potential of proposed experiments are reviewed in this context, with significant emphasis on proposals favored by recent panel reviews. The importance of and challenges in the calculation of nuclear matrix elements that govern the decay are considered in detail. The increasing sensitivity of experiments and improvements in nuclear theory make the future exciting for this field at the interface of nuclear and particle physics.
Avignone, Frank T. III; Elliott, Steven R.; Engel, Jonathan [Department of Physics and Astronomy, University of South Carolina, Columbia, South Carolina 29208 (United States); Los Alamos National Laboratory, Los Alamos, New Mexico 87545 (United States); Department of Physics and Astronomy, University of North Carolina, Chapel Hill, North Carolina 27599-3255 (United States)
Neutrinoless double {beta}-decay and neutrino mass hierarchies
In the framework of the seesaw mechanism the normal hierarchy is favorable for the neutrino mass spectrum. For this spectrum we present a detailed calculation of the half-lives of neutrinoless double {beta}-decay for several nuclei of experimental interest. The half-lives are evaluated by considering the most comprehensive nuclear matrix elements, which were obtained within the renormalized quasiparticle random phase approximation by the Bratislava-Caltech-Tuebingen group. The dependence of the half-lives on sin{sup 2}{theta}{sub 13} and the lightest neutrino mass is studied. We present also the results of the calculations of the half-lives of neutrinoless double {beta}-decay in the case of the inverted hierarchy of neutrino masses.
Bilenky, S. M. [Scuola Internazionale Superiore di Studi Avanzati, I-34014 Trieste (Italy); Faessler, Amand; Gutsche, Thomas; Simkovic, Fedor [Institute fuer Theoretische Physik der Universitaet Tuebingen, D-72076 Tuebingen (Germany)
Unparticle effects on neutrinoless and neutrino double beta decay
The neutrinoless and neutrinos double beta decays are investigated in an effective theory where a scalar unparticle is introduced. We calculate the contributions of a virtual unparticle to the rates of 0{nu}2{beta} and 2{nu}2{beta} decays. The unparticle effect is negligibly small when the scaling dimension d of unparticle lies in the region 1.1-1.9. But it can be relatively large when d approaches to 1 or 2. We also study the neutrinoless double beta decay with a real unparticle in the final state which cannot be distinguished from 2{nu}2{beta} decay in experiment. We consider the unparticle effect on extracting the neutrino mass parameter m{sub {beta}}{sub {beta}} from experimental data and find that it can be relatively large when d takes certain values.
Zhang Chunxu; Huang Mingqiu; Zhong Ming [Department of Physics, National University of Defense Technology, Hunan 410073 (China)
Development and current status of electric double-layer capacitors
An electric double layer capacitor (EDLC) based on the charge storage at the interface between a high surface area carbon electrode and an electrolyte solution is widely used as maintenance-free power source for IC memories and microcomputers. New applications for electric double-layer capacitors have been proposed in recent years. The popularity of these devices is derived from their high energy density relative to conventional capacitors and their long cycle life and high power density relative to batteries. In this paper a classification and a characteristics of industrially produced Japanese small EDLCs are reviewed. Structure and performance of power capacitors under development as well as materials and performance of industrially produced small capacitors are discussed.
Morimoto, Takeshi; Hiratsuka, Kazuya; Sanada, Yasuhiro [Asahi Glass Co. Ltd., Yokohama (Japan). Research Center; Kurihara, Kaname [ELNA Co., Ltd., Fujisawa (Japan)
Double Shell Tank (DST) Process Waste Sampling Subsystem Definition Report
This report defines the Double-Shell Tank (DST) Process Waste Sampling Subsystem (PWSS). This subsystem definition report fully describes and identifies the system boundaries of the PWSS. This definition provides a basis for developing functional, performance, and test requirements (i.e., subsystem specification), as necessary, for the PWSS. The resultant PWSS specification will include the sampling requirements to support the transfer of waste from the DSTs to the Privatization Contractor during Phase 1 of Waste Feed Delivery.
241-SY Double Shell Tanks (DST) Integrity Assessment Report
This report presents the results of the integrity assessment of the 241-SY double-shell tank farm facility located in the 200 West Area of the Hanford Site. The assessment included the design evaluation and integrity examinations of the tanks and concluded that the facility is adequately designed, is compatible with the waste, and is fit for use. Recommendations including subsequent examinations, are made to ensure the continued safe operation of the tanks.
From dark matter to neutrinoless double beta decay
Associated with two TeV-scale leptoquark scalars, a dark matter fermion which is the neutral component of an isotriplet can mediate a testable neutrinoless double beta decay at one-loop level. The dark matter fermion with determined mass and spin-independent scattering can be verified by the future dark matter direct detection experiments. We also discuss the implications on neutrino masses and baryon asymmetry.
The Drinfeld double gl(n) \\oplus t_n
The two isomorphic Borel subalgebras of gl(n), realized on upper and lower triangular matrices, allow us to consider the gl(n) \\opus t_n algebra as a self-dual Drinfeld double. Compatibility conditions impose the choice of an orthonormal basis in the Cartan subalgebra and fix the basis of gl(n). A natural Lie bialgebra structure on gl(n) is obtained, that offers a new perspective for its standard quantum deformation.
A. Ballesteros; E. Celeghini; M. A. del Olmo
Double Pair Production by Ultra High Energy Cosmic Ray Photons
With use of CompHEP package we've made the detailed estimate of the influence of double e+e- pair production by photons (DPP) on the propagation of ultra high energy electromagnetic cascade. We show that in the models in which cosmic ray photons energy reaches few thousand EeV refined DPP analysis may lead to substantial difference in predicted photon spectrum compared to previous rough estimates.
S. V. Demidov; O. E. Kalashev
241-AY Double Shell Tanks (DST) Integrity Assessment Report
This report presents the results of the integrity assessment of the 241-AY double-shell tank farm facility located in the 200 East Area of the Hanford Site. The assessment included the design evaluation and integrity examinations of the tanks and concluded that the facility is adequately designed, is compatible with the waste, and is fit for use. Recommendations including subsequent examinations. are made to ensure the continued safe operation of the tanks.
Nonperturbative theory of double photoionization of the hydrogen molecule
We present completely ab initio nonperturbative calculations of the integral and single differential cross sections for double photoionization of H2 for photon energies from 53.9 to 75.7 eV. The method of exterior complex scaling, implemented with B-splines, is used to solve the Schrodinger equation for a correlated continuum wave function corresponding to a single photon having been absorbed by a correlated initial state. The results are in good agreement with experimental integral cross sections.
Vanroose, W.; Martin, F.; Rescigno, T.N.; McCurdy, C.W.
241-AZ Double Shell Tanks (DST) Integrity Assessment Report
This report presents the results of the integrity assessment of the 241-A2 double-shell tank farm facility located in the 200 East Area of the Hanford Site. The assessment included the design evaluation and integrity examinations of the tanks and concluded that the facility is adequately designed, is compatible with the waste, and is fit for use. Recommendations including subsequent examinations, are made to ensure the continued safe operation of the tanks.
Neutrino mass matrix solutions and neutrinoless double beta decay
We present a determination of the neutrino mass matrix which holds for values of the neutrinoless double beta decay effective mass m_{ee} larger than the neutrino mass differences. We find eight possible solutions and discuss for each one the corresponding neutrino mass eigenvalues and zero texture. A minimal structure of the perturbations to add to these zero textures to recover the full mass matrix is also determined. Implications for neutrino hot dark matter are discussed for each solution.
Thomas Hambye
Observable Majoron Emission in Neutrinoless Double Beta Decay
We consider a class of simplest Majoron models where neutrino- majoron couplings can be in the range $g \\sim 10^{-5}-10^{-3}$ leading to the observability of neutrinoless double beta decay with majoron emission. The majoron is a singlet of the electroweak gauge symmetry, thus avoiding conflict with the LEP data on Z decay, which rules out the triplet and doublet majoron models.
Z Berezhiani; A Yu Smirnov; J W F Valle
Double dielectric passivation layer on InAs
) August 1985 ABSTRACT Double Dielectric Passivation Layer on InAs. (August 1985) James Lee Egley, B. S. , Lewis University Chairman of Advisory Committee: Yellapu Anjaneyulu This thesis investigates the characteristics of the substrate surface... as the lubricant. The wafers were polished to a mirror-like finish. An alumina slurry was also tried for this step, but it was found that the chemicals contained in the slurry etched the surface. After the above mechanical polishing the samples were removed...
Egley, James Lee
Double Diffusion in Enclosure Bounded by Massive and Volatilizing Walls
ICEBO2006, Shenzhen, China Maximize Comfort: Temperature, Humidity and IAQ Vol.I-6-5 Double Diffusion in Enclosure Bounded by Massive and Volatilizing Walls Di Liu Guangfa Tang Fuyun Zhao Doctoral Professor.... INTRODUCTION It has become evident that building products are major contributors to the pollution of the indoor air environment with volatile organic compounds (VOCs) [1]. The indoor airflow and temperature distributions also have influence on the emission...
Liu, D.; Tang, G.; Zhao, F.
Recent advances in neutrinoless double beta decay search
Even after the discovery of neutrino flavour oscillations, based on data from atmospheric, solar, reactor, and accelerator experiments, many characteristics of the neutrino remain unknown. Only the neutrino square-mass differences and the mixing angle values have been estimated, while the value of each mass eigenstate still hasn't. Its nature (massive Majorana or Dirac particle) is still escaping. Neutrinoless double beta decay ($0\
Lino Miramonti; Franco Reseghetti
Charged Majoron Emission in Neutrinoless Double Beta Decay
We examine in detail the predictions of the charged majoron model, introduced recently by Burgess and Cline, for 0+ --> 0+ double beta decay transitions. The relevant nuclear matrix elements are evaluated, within the quasiparticle random phase approximation, for 76Ge, 82Se, 100Mo, 128Te and 150Nd nuclei. The calculated transition rates turn out to be much smaller than the experimental upper limits on possible majoron emission, except in a small region of the model's parameter space.
C. Barbero; J. M. Cline; F. Krmpotic; D. Tadic
Neutrinoless double beta decay and direct searches for neutrino mass
Study of the neutrinoless double beta decay and searches for the manifestation of the neutrino mass in ordinary beta decay are the main sources of information about the absolute neutrino mass scale, and the only practical source of information about the charge conjugation properties of the neutrinos. Thus, these studies have a unique role in the plans for better understanding of the whole fast expanding field of neutrino physics.
Craig Aalseth; Henning Back; Loretta Dauwe; David Dean; Guido Drexlin; Yuri Efremenko; Hiro Ejiri; Steven Elliott; Jon Engel; Brian Fujikawa; Reyco Henning; G. W. Hoffmann; Karol Lang; Kevin Lesko; Tadafumi Kishimoto; Harry Miley; Rick Norman; Silvia Pascoli; Serguey Petcov; Andreas Piepke; Werner Rodejohann; David Saltzberg; Sean Sutton; Petr Vogel; Ray Warner; John Wilkerson; Lincoln Wolfenstein
Neutrinoless double beta decay studied with configuration mixing methods
We study neutrinoless double beta decay of several isotopes with state-of-the-art beyond self-consistent mean field methods to compute the nuclear matrix elements (NME). Generating coordinate method with particle number and angular momentum projection (GCM+PNAMP) is used for finding mother and granddaughter states and evaluating transition operators between different nuclei. We analyze explicitly the role of the deformation, pairing and configuration mixing in the evaluation of the NME.
Tomas R. Rodriguez; Gabriel Martinez-Pinedo
Nuclear Structure Aspects of Neutrinoless Double Beta Decay
We decompose the neutrinoless double-beta decay matrix elements into sums of products over the intermediate nucleus with two less nucleons. We find that the sum is dominated by the J^pi=0^+ ground state of this intermediate nucleus for both the light and heavy neutrino decay processes. This provides a new theoretical tool for comparing and improving nuclear structure models. It also provides the connection to two-nucleon transfer experiments.
B. A. Brown; M. Horoi; R. A. Sen'kov
Brown, B A; Sen'kov, R A
Double sided circuit board and a method for its manufacture
Conductance between the sides of a large double sided printed circuit board is provided using a method which eliminates the need for chemical immersion or photographic exposure of the entire large board. A plurality of through-holes are drilled or punched in a substratum according to the desired pattern, conductive laminae are made to adhere to both sides of the substratum covering the holes and the laminae are pressed together and permanently joined within the holes, providing conductive paths. 4 figs.
Lindenmeyer, C.W.
Differential cross sections of double photoionization of lithium
We extend our previous application of the convergent close-coupling (CCC) and time-dependent close-coupling (TDCC) methods [Phys. Rev. A 81, 023418 (2010)] to describe energy and angular resolved double photoionization (DPI) of lithium at arbitrary energy sharing. By doing so, we are able to evaluate the recoil ion momentum distribution of DPI of Li and make a comparison with recent measurements of Zhu et al. [Phys. Rev. Lett. 103, 103008 (2009)].
Kheifets, A. S.; Fursa, D. V.; Bray, I.; Colgan, J.; Pindzola, M. S. [Research School of Physical Sciences, Australian National University, Canberra, Australian Capital Territory 0200 (Australia); Institute of Theoretical Physics, Curtin University, Perth, Western Australia 6845 (Australia); Theoretical Division, Los Alamos National Laboratory, Los Alamos, New Mexico 87545 (United States); Department of Physics, Auburn University, Auburn, Alabama 36849 (United States)
Double-clad nuclear-fuel safety rod
McCarthy, W.H.; Atcheson, D.B.
THE PROTOTYPE ALUMINUM - CARBON SINGLE, DOUBLE, AND TRIPLE BONDS: Al - CH3, Al = CH2, AND Al. = CH
three prototype Table II. aluminum-carbon bonds and theirPhysics THE PROTOTYPE ALUMINUM - CARBON SINGLE, DOUBLE, ANDLBL-l0871 The Prototype Aluminum - Carbon Single, Double.
Fox, Douglas J.
Multisublevel Magnetoquantum Conductance in Single and Coupled Double Quantum Wires
We study the ballistic and diffusive magnetoquantum transport using a typical quantum point contact geometry for single and tunnel-coupled double wires that are wide (less than or similar to1 mum) in one perpendicular direction with densely populated sublevels and extremely confined in the other perpendicular (i.e., growth) direction. A general analytic solution to the Boltzmann equation is presented for multisublevel elastic scattering at low temperatures. The solution is employed to study interesting magnetic-field dependent behavior of the conductance such as a large enhancement and quantum oscillations of the conductance for various structures and field orientations. These phenomena originate from the following field-induced properties: magnetic confinement, displacement of the initial- and final-state wave functions for scattering, variation of the Fermi velocities, mass enhancement, depopulation of the sublevels and anticrossing (in double quantum wires). The magnetoconductance is strikingly different in long diffusive (or rough. dirty) wires from the quantized conductance in short ballistic (or clean) wires. Numerical results obtained for the rectangular confinement potentials in the growth direction are satisfactorily interpreted in terms of the analytic solutions based on harmonic confinement potentials. Some of the predicted features of the field-dependent diffusive and quantized conductances are consistent with recent data from GaAs/AlxGa1-xAs double quantum wires.
Lyo, Sungkwun Ken; Huang, Danhong
Neutrinoless double-beta decay searches play a major role in determining the nature of neutrinos, the existence of a lepton violating process, and the effective Majorana neutrino mass. The Majorana Collaboration proposes to assemble an array of HPGe detectors to search for neutrinoless double-beta decay in Ge-76. Our proposed method uses the well-established technique of searching for neutrinoless double-beta decay in high purity Ge-diode radiation detectors that play both roles of source and detector. The technique is augmented with recent improvements in signal processing and detector design, and advances in controlling intrinsic and external backgrounds. Initially, Majorana aims to construct a prototype module containing 60 kg of Ge detectors to demonstrate the potential of a future 1-tonne experiment. The design and potential reach of this prototype Demonstrator module will be presented. This paper will also discuss detector optimization and low-background requirements, such as material purity, background rejection, and identification of rare backgrounds required to reach the sensitivity goals of the Majorana experiment.
V. E. Guiseppe; for the Majorana Collaboration
Methotrexate intercalated ZnAl-layered double hydroxide
The anticancerous drug methotrexate (MTX) has been intercalated into an ZnAl-layered double hydroxide (LDH) using an anion exchange technique to produce LDH-MTX hybrids having particle sizes in the range of 100-300 nm. X-ray diffraction studies revealed increases in the basal spacings of ZnAl-LDH-MTX hybrid on MTX intercalation. This was corroborated by the transmission electron micrographs, which showed an increase in average interlayer spacing from 8.9 A in pristine LDH to 21.3 A in LDH-MTX hybrid. Thermogravimetric analyses showed an increase in the decomposition temperature for the MTX molecule in the LDH-MTX hybrid indicating enhanced thermal stability of the drug molecule in the LDH nanovehicle. The cumulative release profile of MTX from ZnAl-LDH-MTX hybrids in phosphate buffer saline (PBS) at pH 7.4 was successfully sustained for 48 h following Rigter-Peppas model release kinetics via diffusion. - Graphical abstract: ZnAl-layered double hydroxide intercalated with methotrexate ({approx}34% loading) promises the possibility of use of ZnAl-LDH material as drug carrier and in controlled delivery. Highlights: > ZnAl-layered double hydroxide methotrexate nanohybrid has been synthesized. > XRD and TEM studies on nanohybrid revealed successful intercalation of methotrexate. > TG and CHN analyses showed {approx}34 wt% of methotrexate loading into the nanohybrid. > Possibility of use of ZnAl-LDH material as drug carrier and in delivery.
Chakraborty, Manjusha; Dasgupta, Sudip; Soundrapandian, Chidambaram [Central Glass and Ceramic Research Institute, CSIR, 196 Raja S.C. Mullick Road, Kolkata 700032 (India); Chakraborty, Jui, E-mail: email@example.com [Central Glass and Ceramic Research Institute, CSIR, 196 Raja S.C. Mullick Road, Kolkata 700032 (India); Ghosh, Swapankumar, E-mail: email@example.com [National Institute for Interdisciplinary Science and Technology (NIIST), CSIR, Trivandrum 695019 (India); Mitra, Manoj K. [Department of Metallurgical and Materials Engineering, Jadavpur University, Kolkata 700032 (India); Basu, Debabrata [Central Glass and Ceramic Research Institute, CSIR, 196 Raja S.C. Mullick Road, Kolkata 700032 (India)
Neutrino masses and Neutrinoless Double Beta Decay: Status and expectations
Two most outstanding questions are puzzling the world of neutrino Physics: the possible Majorana nature of neutrinos and their absolute mass scale. Direct neutrino mass measurements and neutrinoless double beta decay (0nuDBD) are the present strategy to solve the puzzle. Neutrinoless double beta decay violates lepton number by two units and can occurr only if neutrinos are massive Majorana particles. A positive observation would therefore necessarily imply a new regime of physics beyond the standard model, providing fundamental information on the nature of the neutrinos and on their absolute mass scale. After the observation of neutrino oscillations and given the present knowledge of neutrino masses and mixing parameters, a possibility to observe 0nuDBDD at a neutrino mass scale in the range 10-50 meV could actually exist. This is a real challenge faced by a number of new proposed projects. Present status and future perpectives of neutrinoless double-beta decay experimental searches is reviewed. The most important parameters contributing to the experimental sensitivity are outlined. A short discussion on nuclear matrix element calculations is also given. Complementary measurements to assess the absolute neutrino mass scale (cosmology and single beta decays) are also discussed.
In this paper, we study the sensitivity of CUORE, a bolometric double-beta decay experiment under construction at the Laboratori Nazionali del Gran Sasso in Italy. Two approaches to the computation of experimental sensitivity are discussed and compared, and the formulas and parameters used in the sensitivity estimates are provided. Assuming a background rate of 10{sup -2} cts/(keV kg y), we find that, after 5 years of live time, CUORE will have a 1#27;{sigma} sensitivity to the neutrinoless double-beta decay half-life of {caret T{sup 0{nu}}{sub 1/2}}(1{sigma}#27;) = 1.6x#2;10{sup 26} y and thus a potential to probe the effective Majorana neutrino mass down to 41-95 meV; the sensitivity at 1.64{sigma}#27;, which corresponds to 90% C.L., will be {caret T{sup 0{nu}}{sub 1/2}(1.64{sigma}#27;}) = 9.5x10{sup 25} y. This range is compared with the claim of observation of neutrinoless double-beta decay in {sup 76}Ge and the preferred range in the neutrino mass parameter space from oscillation results.
CUORE; Alessandria, F.; Andreotti, E.; Ardito, R.; Arnaboldi, C.; Avignone III, F. T.; Balata, M.; Bandac, I.; Banks, T. I.; Bari, G.; Beeman, J.; Bellini, F.; Bersani, A.; Biassoni, M.; Bloxham, T.; Brofferio, C.; Bryant, A.; Bucci, C.; Cai, X. Z.; Canonica, L.; Capelli, S.; Carbone, L.; Cardani, L.; Carrettoni, M.; Casali, N.; Chott, N.; Clemenza, M.; Cosmelli, C.; Cremonesi, O.; Creswick, R. J.; Dafinei, I.; Dally, A.; Biasi, A. De; Decowski, M. P.; Deninno, M. M.; Waard, A. de; Domizio, S. Di; Ejzak, L.; Faccini, R.; Fang, D. Q.; Farach, H. A.; Ferri, E.; Ferroni, F.; Fiorini, E.; Foggetta, L.; Franceschi, M. A.; Freedman, S. J.; Frossati, G.; Fujikawa, B. K.; Giachero, A.; Gironi, L.; Giuliani, A.; Gorla, P.; Gotti, C.; Guardincerri, E.; Gutierrez, T. D.; Haller, E. E.; Han, K.; Heeger, K. M.; Huang, H. Z.; Ichimura, K.; Kadel, R.; Kazkaz, K.; Keppel, G.; Kogler, L.; Kolomensky, Yu. G.; Kraft, S.; Lenz, D.; Li, Y. L.; Liu, X.; Longo, E.; Ma, Y. G.; Maiano, C.; Maier, G.; Maino, M.; Mancini, C.; Martinez, C.; Martinez, M.; Maruyama, R. H.; Moggi, N.; Morganti, S.; Napolitano, T.; Newman, S.; Nisi, S.; Nones, C.; Norman, E. B.; Nucciotti, A.; Orio, F.; Orlandi, D.; Ouellet, J. L.; Pallavicini, M.; Palmieri, V.; Pattavina, L.; Pavan, M.; Pedretti, M.; Pessina, G.; Pirro, S.; Previtali, E.; Rampazzo, V.; Rimondi, F.; Rosenfeld, C.; Rusconi, C.; Salvioni, C.; Sangiorgio, S.; Schaeffer, D.; Scielzo, N. D.; Sisti, M.; Smith, A. R.; Stivanello, F.; Taffarello, L.; Terenziani, G.; Tian, W. D.; Tomei, C.; Trentalange, S.; Ventura, G.; Vignati, M.; Wang, B. S.; Wang, H. W.; Whitten Jr., C. A.; Wise, T.; Woodcraft, A.; Xu, N.; Zanotti, L.; Zarra, C.; Zhu, B. X.; Zucchelli, S.
An Accelerated Recursive Doubling Algorithm for Block Tridiagonal Systems
Block tridiagonal systems of linear equations arise in a wide variety of scientific and engineering applications. Recursive doubling algorithm is a well-known prefix computation-based numerical algorithm that requires O(M^3(N/P + log P)) work to compute the solution of a block tridiagonal system with N block rows and block size M on P processors. In real-world applications, solutions of tridiagonal systems are most often sought with multiple, often hundreds and thousands, of different right hand sides but with the same tridiagonal matrix. Here, we show that a recursive doubling algorithm is sub-optimal when computing solutions of block tridiagonal systems with multiple right hand sides and present a novel algorithm, called the accelerated recursive doubling algorithm, that delivers O(R) improvement when solving block tridiagonal systems with R distinct right hand sides. Since R is typically about 100 1000, this improvement translates to very significant speedups in practice. Detailed complexity analyses of the new algorithm with empirical confirmation of runtime improvements are presented. To the best of our knowledge, this algorithm has not been reported before in the literature.
Seal, Sudip K [ORNL] [ORNL
A New Merging Double Degenerate Binary in the Solar Neighborhood
Characterizing the local space density of double degenerate binary systems is a complementary approach to broad sky surveys of double degenerates to determine the expected rates of white dwarf binary mergers, in particular those that may evolve into other observable phenomena such as extreme helium stars, Am CVn systems, and supernovae Ia. However, there have been few such systems detected in local space. We report here the discovery that WD 1242$-$105, a nearby bright WD, is a double-line spectroscopic binary consisting of two degenerate DA white dwarfs of similar mass and temperature, despite it previously having been spectroscopically characterized as a single degenerate. Follow-up photometry, spectroscopy, and trigonometric parallax have been obtained in an effort to determine the fundamental parameters of each component of this system. The binary has a mass ratio of 0.7 and a trigonometric parallax of 25.5 mas, placing it at a distance of 39 pc. The system's total mass is 0.95 M$_\\odot$ and has an orbita...
Debes, John H; Tremblay, Pier-Emmanuel; L�pez-Morales, Mercedes; Anglada-Escud�, Guillem; Napiwotzki, Ralf; Osip, David; Weinberger, Alycia
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