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56 year old man | La Habra, CA, USA | Seeking women 42-52 within 25 miles
I enjoy laughter, passion and communication. Life is at it's best when shared.
Hi, thanks for stopping by. I think if I were to ask my friends to describe me I think first and foremost they would say I am someone they can always count on. I am non-judgmental, a good listener and honest with advise. I am a person who loves to laugh and enjoy the positives in life. Life is way too damn short. I enjoy doing or trying just about anything. From sports to dinners, small venues with live entertainment, wine tasting, the beach and great conversation. I have a great career that I enjoy very much in health care. I am proud and extremely grateful for my 4 kids. The 2 oldest have graduated college and have great careers. I am looking for a women who is passionate, funny, adventurous, family oriented, loyal, enjoys communicating and someone who ultimately wants a relationship. I am not a serial dater nor do I want to be on the other end. I try to eat healthy (for the most part), and exercise 3 to 4 times a week. If any of this sounds good please drop me a note. I truly believe life is better when shared.
Camping , Coffee and conversation , Cooking , Dining out , Gardening/Landscaping , Movies/Videos , Music and concerts , Exploring new areas , Nightclubs/Dancing , Performing arts , Playing cards , Playing sports , Political interests , Religion/Spiritual , Shopping/Antiques , Travel/Sightseeing , Volunteering , Watching sports , Wine tasting
Baseball, Basketball, Billiards / Pool, Bowling, Dancing, Football, Golf, Swimming, Tennis / Racquet sports, Walking / Hiking, Weights / Machines
I exercise 3-4 times per week.
Don't display my sign
I like Dogs, Fish, Horses
Middle of the Road
Dinner in the beach cities, Las Vegas, Santa Barbara, Wine Country, San Diego with the kids and Hawaii.
I enjoy most all music. From oldies to rock, jazz to country. Techno is never a pandora selection for me.
I like to play tennis with my oldest son. Basketball and golf are interests. Movies of all kinds, dinners, wine tasting, small venues with live entertainment are things I enjoy. Walking on the beach and watching the sunset is great.
Newspaper daily, business journals and clinical laboratory publications.
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What he is looking for
|6'4" (193cms)
|5'2" (157cms) to 6'0" (182cms)
|Body type:
|About average
|Slender, About average, Athletic and toned
|No preference
|Dark brown
|No preference
|No Way
|No Way
|Social Drinker
|Social Drinker
|Medical / Dental / Veterinary / Fitness
|No preference
|No preference
|No preference
|Have kids:
|Yes, and they sometimes live at home (More than 3)
|No preference
|Wants kids:
|No, but it's OK if my partner has kids
|No preference
|White / Caucasian
|Asian, Latino / Hispanic, Middle Eastern, Native American, Pacific Islander, White / Caucasian
My Grandparents on both sides were born in Italy.
|Christian / Other
|No preference
|Bachelors degree
|No preference


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Posted 2 years ago in Information Technology
Synergy Staffing is currently accepting resumes for an Automated QA Contractor position located in Wexford, PA. Please see below for additional details...
Synergy Staffing, Inc. is an innovative leader in the Western Pennsylvania recruiting and placement industry. We are committed to understanding our clients needs and providing solutions that not only meet, but exceed their expectations. We match thoroughly vetted candidates to Contract, Contract-to-Hire, and Permanent positions in the Information Technology and Professional Services industries.
Browse quality assurance analyst jobs in Pittsburgh, Pennsylvania:
Oxford Solutions - Pittsburgh, PA
Posted 9 days ago on ZipRecruiter - ZipApply
Kelly Services - Pittsburgh, PA
Posted 4 days ago on the web
Oxford Solutions - Pittsburgh, PA
Posted 20 days ago on ZipRecruiter - ZipApply
See all open Quality Assurance Analyst Jobs in Pittsburgh, Pennsylvania

subset: commoncrawl


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*Change for 2014-2015
Chemeketa Community College will be waiving the mandatory prerequisites for high school students who enroll in College Credit Now courses. This waiver extends only to those students who are enrolling in CCN courses and does not apply to regular admission or Chemeketa Scholars Program application. The COMPASS Placement test waiver, signed by the authorized District agent, will be in effect beginning Fall 2014. This is with the understanding and agreement that the high school will be responsible for monitoring and ensuring that students have met the mandatory prerequisites by completing the equivalent high school course prior to enrolling in a College Credit Now class.


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|Home > Entertaining > Raclette & Fondue > Fondue > Emile Henry Red Flame-Top Cheese Fondue Set
This item is in our Open Box section. In all likelihood, it is a floor sample. Although it has never been used, some scratches, dents, torn packaging, or other imperfections are possible.
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John Silvester Google+
John Silvester is a Walkley-award winning crime writer and columnist. A co-author of the best-selling books that formed the basis of the hit Australian TV series Underbelly, Silvester is also a regular guest on 3AW with his "Sly of the Underworld" segment.
Terror casts a long shadow
John Silvester They are meatballs, not martyrs.
Naked City: Tip of the Iceberg
John Silvester Tony Abbott deserves credit for making a national response to the national ice problem.
Changing culture: When good cops tackle bad cops
John Silvester Sexual predators in the Victoria Police have used their rank and a code of silence to protect themselves from exposure and punishment.
From Russia With Love
John Silvester Tall tale proves a Tsar too far for a Queensland court.
Plan by police to quiz Carl Williams about gangland murders was discovered by criminals
Nick McKenzie, Richard Baker, John Silvester A police plan to "download" Carl Williams about his knowledge of unsolved gangland murders was discovered by criminals, including a gangland figure who was able to easily breach jail security to...
Naked City: Ken Lay lays down the law
John Silvester They call him "Whispering Ken" but after three years in the job Chief Commissioner Ken Lay is living proof that in an era where the loudest voices get heard the old-fashioned rule, "Speak softly and...
Naked City - Looking the other way on family violence has to stop
John Silvester We are one of the safest countries in the world and yet one woman is killed every week due to family violence. It is a national disgrace and we should all be deeply ashamed.
Stolen car rorts exposed in clampdown as cops change tack
John Silvester A police clampdown is making the re-birthing of cars a thing of the past but a loophole has made it easier to steal older cars, now worth about three times as much as spare parts.
Naked city: Police taskforces are nothing new
John Silvester John Silvester examines the many Victoria Police taskforces into drugs gangs and organised crime and asks 'Are they working?'
Naked city: Special Ops vet and Patch do the hard yards
John Silvester Tough guys shouldn't own fluffy poodle-type pups, particularly one with a ridiculously cute name such as Patch.
Nowhere to hide
John Silvester A rapist is brought to justice - after nearly 20 long and fear-filled years for the victim.
Power, violence and the laws of average
John Silvester Laws are there to reflect community standards, not impose them so, if we can't behave in a non-violent manner, no amount of court inspired threats will make a dunghill of difference.
A force to be reckoned with
John Silvester There is a union that even the right-wingers seem to love. It doesn't strike, only veers left at traffic lights, and the only green cause it embraces is drinking beer on St Patrick's Day.
The case for Julian Knight
John Silvester On one hand mass-killer Julian Knight is the perfect candidate for parole.
Australia caught up in a global operation
John Silvester No one could work out why the second tier football side filled with internationals would play so badly.
Mafia insider hid role in hit on campaigner
John Silvester, Nick Ralston and Lisa Davies A Mafia insider who told police he hired the hitman who killed Griffith anti-drugs campaigner Donald Mackay was in fact present at the 1977 execution, and helped dump the body in a well on a property...
Killers dumped Mackay in well, police told
John Silvester Exclusive NSW police reinvestigating the 36-year-old murder of Griffith anti-drugs campaigner Donald Mackay have been told two hit men dumped the body in a well at a property near Hay on their way...
Bottom feeder who should never have been allowed on the street
John Silvester Jill Meagher should have woken up that Saturday morning with a slight hangover and pleasant memories of a night on the town with her workmates.
Football insider lifts lid on drugs
John Silvester A controversial sports biochemist says an organised crime network wanted him to spike AFL players' supplements with illicit drugs as part of a plan to blackmail them into joining a match-fixing ring.
Meagher accused set to change plea
John Silvester The man charged with raping and murdering Jill Meagher is expected to change his plea to guilty of murder in the Supreme Court on Friday.


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Inner Richmond
Cafe Bunn Mi in The Inner Richmond
Next trip: The Crispy Duck Sandwich It would take a dog's life span to experience everything that Clement Street in the Inner Richmond has to offer, culinary and otherwise. That's why being in the restaurant business and/or having friends in said business helps immeasurably. Recently, a work friend recommended Café
Wing Lee Bakery: Treasures for the Adventurous…
There are few brave enough to venture out into the Richmond district, even if it's the Inner Richmond which is like four blocks from Upper Haight. If you've lived here long enough, you can hear the gasps of neighborhood-elitists when you say, "I live out in the Richmond/Sunset." I honestly
There is More to Korean Cuisine Than Just BBQ!
You know a place is worth visiting after Anthony Bourdain and Chris Cosentino gave it a thumbs up on the show "The Layover". To Hyang on Geary and 2nd Ave. in the Inner Richmond is no exception. After seeing a short video Bourdain made about the place, I decided to try it out with
FREE Chowder and Tunes at Frankenart Mart
This time last week, I went to Frankenart Mart for the first time and (just as importantly) my first free hot dog in our fair city. Sausages abound in San Francisco, if you know where to look, but the good old-fashioned flaccid, terracotta-hued wieners? A little harder to come across.
4th Annual Inner Richmond Pubcrawl
The Richmond gets a bad rap. Actually, it gets no rap, really. Despite the fact that the RD is home to tasties (Shanghai Dumpling King? Eh?), the biggest festivals (Hardly Strictly? Eh?), Robin Williams (kind of – he lives in Seacliff), extra strong coffee to combat all the fog, and
Delicious Dim Sum Deals
The other morning I was in the Inner Richmond and stopped in for some delicious Chinese pastries at Good Luck Dim Sumon Clement. The smell of these baked, steamed and fried little dumplings was out of control. There was a super long line of locals patiently waiting for their breakfast
Ireland's 32 is Just Plain Awesome
People come to Ireland's 32 to get drunk. Period. I'm not talking a little stumble and a brick-scraped elbow on the way home drunk. I'm talking blackout, after hours bacon-wrapped hot dog stained shirt, pass out on top of your bed fully clothed, worst hangover headache in a while drunk.


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Amherst College "IT Index" Tracks Student, Campus Tech Use Over Time
September 26, 2008
AMHERST, Mass.—It's a new academic year at Amherst College, which means a new class of undergraduates with new attitudes and aptitudes about technology—and, of course, new gadgets. Peter Schilling, the college's director of information technology, provides the following snapshot of how students use technology and how wired the college is. Modeled after the Harper's Index feature in Harper's Magazine, Schilling calls it his own Amherst IT Index.
• Percentage of applicants who applied for admission to Amherst online in 2003: 33 percent.
• Percentage of applicants who did the same in 2007: 89 percent.
• Year that members of an incoming Amherst class first created a Facebook group so that they could get to know each other prior to arriving on campus: 2006.
• Total number of members and posts on the Amherst Class of 2012 Facebook group page by the end of August 2008: 432 members and 3,225 posts out of a class of 438 students.
• Students in the Class of 2012 who registered computers, iPhones, game consoles and other devices on the campus network by the end of Sunday, August 24, the day they moved into their dorm rooms: 370 students registered 443 devices.
• Number of students in the Class of 2012 who brought desktop computers to campus: 14.
• Number of students who brought iPhones/iTouches: 93.
• Likelihood that an Amherst undergraduate with an iPhone/iTouch is a member of the class of 2012: approximately 1 in 2.
• Total number of students on campus this year with landline phone service: 5.
• Of the four classes currently on campus, the Classes of 2009 and 2010 are more likely to own PCs, while the Classes of 2011 and 2012 are more likely to own Macs.
• Total bandwidth to and from the Internet available on campus in September 2000: 3 megabits per second.
• Total bandwidth to and from the Internet available on campus today: 100 megabits per second for the Internet and 45 for Internet 2.
• Percentage of Amherst classrooms that have an LCD projector and a computer or laptop hookup: 85 percent.
• Number of individual film titles in the college's digital video streaming collection: 1,260.
• Number of times these films were watched last year: 20,662.
• Average number of e-mails received on campus per day: 180,000.
• Percentage of total e-mail that arrives as spam: 94 percent.
• Percentage of storage space taken up by e-mail—a system designed to send brief text messages—that is actually taken up by the files attached to the emails: 95 percent. (The storage required for e-mail and attachments for the year 2007 alone was equal to that of the preceding five years together.)
• Increase in the total number of college-owned computers in use on campus from 2005 to 2008: 413, for a 2008 total of 1,308.
• Total number of calls and e-mails to the IT Help Desk in 2007: 8,650.
• Average time required to close a help-desk ticket: 39 minutes.
• Based on the first nine months of 2008, estimated percentage increase in Help Desk tickets in 2008 over 2007: 11 percent (or 15.6 weeks of work for a staff member).
• Job applicants interviewed for 12 openings in IT in the last year who learned about Amherst positions through postings in regional, national or professional, publications, Web sites or lists: 1.


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BRUNSWICK - The eight people slain in a weekend mobile home attack in coastal Georgia included a father and four older children and other members of an extended family, police and relatives said Tuesday.
BRUNSWICK - A frantic caller told authorities he had just come home to find several relatives apparently beaten to death and another barely breathing, according to a 911 tape released Monday from the weekend attack at a mobile home park in southeastern Georgia.
"My whole family is dead!" screamed Guy Heinze Jr., 22. "It looks like they've been beaten to death. I don't know what to do, man."
BRUNSWICK - Toni Mugavin says she wonders if she needs to sleep with a gun under her pillow, afraid the killer of eight people at a Georgia mobile home is still on the loose and frustrated with the lack of information about what happened.
The eighth casualty came Sunday, a day after police discovered seven bodies and two survivors in one mobile home in Brunswick. The latest victim was Michael Toler, 19, who lived in the home and had been hospitalized Saturday. He is the first and only victim police have named.
BRUNSWICK - The man who called 911 to report finding seven people slain in a dingy mobile home on a historic Georgia plantation was arrested on drug-related charges, though police refused to say Sunday whether he was a suspect in the killings.
Two people survived the attack, with brutal injuries.
ATLANTA - The head of Georgia's social services department says a 12-day furlough plan won't affect critical services because they will still be available online.
Department of Human Services Commissioner B.J. Walker said child welfare and adult protective services will be available online during the 12 furlough days when the agency will completely shut down over the next year.
The Georgia Department of Revenue delivered 34,000 more refunds to residents last week and is on schedule to process more returns by the end of the month, according to a recent update from the department.
ATLANTA (AP) - The grand prize in the Mega Millions multistate lottery game is growing fatter still, to $325 million for the next drawing on Friday.
The jackpot has been rolled over because no player matched all the winning numbers in Tuesday's $252 million drawing.
ATLANTA - Three Georgia lawmakers could face sanctions for failing to pay their state taxes under a new law that gave ethics officials power to investigate and penalize delinquent legislators.
SAVANNAH - Savannah-Chatham County police chief Michael Berkow has announced his resignation.
Berkow was hired as chief in 2006 and is leaving after less than three years on the job.
Dalton Raulerson, the Pembroke teen who was struck by lightning Aug. 5 during football practice at Pinewood Christian Academy, was released from the hospital Thursday and is home.
The Armstrong Center for Professional and Continuing Education will host a free open house on Wednesday, Sept. 9, from 4-6:30 p.m. at 13040 Abercorn St., Savannah.
ATLANTA - House lawmakers have approved Georgia's first transportation planning director.
The House Transportation Committee voted unanimously Wednesday to make Todd Long the Department of Transportation's first director of planning.
ATLANTA - A Georgia Department of Transportation official says the DOT's budget is balanced for now, but faces an uncertain future.
DOT Treasurer Kate Pfirman told board members at their monthly meeting Wednesday that the state's budget from gas taxes balanced out exactly for the fiscal year that ended June 30.
ATLANTA - The Supreme Court on Monday ordered a new hearing for death row inmate Troy Davis, giving the condemned killer a chance to present evidence his lawyers say could clear him in the murder of an off-duty police officer almost 20 years ago.
The court told a federal judge to hold a hearing to decide whether evidence "that could not have been obtained at the time of trial" could establish Davis' innocence. His case has become a rallying cry for death penalty opponents.
BRUNSWICK - Another delay is pushing back the death penalty trial of a man charged with sexual assault and murder in the 2007 slaying of a 6-year-old Brunswick boy.
Superior Court Judge Stephen Scarlett has ordered jury selection in the trial of David Edenfield to begin Sept. 21, nearly two weeks later than a date the judge scheduled months ago.
Even before the Supreme Court declared same-sex marriage a constitutional right last week, there was an aura of inevitability about the outcome that would have been hard to anticipate just seven years ago.
A proposal to revamp New York state's prison sentencing system will not just save taxpayers money and restore fairness, but it will also help families of inmates cope with the social and economic impacts of having a parent behind bars.
Earlier this month, federal authorities cracked down on four cancer charities that had bilked millions of dollars from donors, and Propublica investigations came down hard on the Red Cross, questioning its use of the half-billion dollars it raised for Haiti relief.
New York isn't the only place where rent is sky high.
America's inventory of affordable housing is not keeping pace with its growing number of "extremely low-income" renters, new research shows.
OVERLAND PARK, Kan. — A picture of a cop feeding a baby after her mom had gone into a seizure has captured the hearts of people around the world.
There's a lot of talk about the political reaction to Pope Francis' 192-page encyclical on climate change. But part of what's being missed, say Catholic leaders, is a message about better caring for communities and families — the "human ecosystems" that allow humans to thrive.
The Supreme Court rejected a bid to hear a case concerning a law that denied bail to undocumented immigrants living in Arizona charged with certain felonies.
Family immigrant detention centers, which serve as holding points for undocumented women and children caught crossing the border, have come under fire in recent weeks.
Michael Hall has worked at Seattle's Space Needle for seven years, taking visitors up and down the attraction's elevator.
As baby boomers roll toward retirement, half of them don't have retirement savings, according to a new report from the U.S. Government Accountability Office.
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Street Food All-Stars Join Forces for New Harlem Market
It all starts this Sunday, and there will also be beer.READ MORE »
It all starts this Sunday, and there will also be beer.READ MORE »
Whoever loses, you win, really.READ MORE »
Plus: Melvin's Juice Box cleanses, and more, in today's Leftovers.READ MORE »
Speculation about speculoos.READ MORE »
A reverse bridge-and-tunnel commute for the Belgian waffles truck.READ MORE »
"Wafel+dulce de leche+cinnamon sugar."READ MORE »
Plus: "Chindian" food is a thing, Senate passes food-safety bill (again), and more, in our morning news roundup.READ MORE »
Plus: Manchester Pub offers lunch special, Long Tan closes in Park Slope, and more, in our daily roundup of neighborhood news.READ MORE »
Plus: Ayza opens for lunch on weekends, and River Barrel Café transforms into Mrs. Kim's, in our daily roundup of neighborhood news.READ MORE »
Plus, is the Hallo Berlin cart coming back?READ MORE »
Or just wear yellow.READ MORE »
The chef tries to beat a waffle maker at his own game.READ MORE »
Plus: an all-Brooklyn coffee shop, and Bobby Flay throws down with Wafels & Dinges, in our morning news roundup.READ MORE »
To celebrate the release of 'This Is Why You're Fat' next week.READ MORE »
Tacos, crêpes, and Swedish meatballs will soon compete with the Shack Burger.READ MORE »
And more winners at Saturday's awards.READ MORE »
The Post lashes out at "twee" food trucks, and the trucks fight back.READ MORE »
Bacon waffles and the world's largest cupcake.READ MORE »
Two weeks after the management of 375 Hudson tried to run off the Wafels & Dinges truck because it "looked unprofessional," it's been spotted outside the building again.READ MORE »
Trucks like Wafels & Dinges, Cupcake Stop, and also the Shake Shack (!) will be giving away freebies on the first day of summer.READ MORE »


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* Polyester, adhesive
* Spot clean
* Imported
* Set of 6
* Package: 8.5"w, 9.5"h
Style #:20666210
Dare to wear facial hair with this set of 6 notable moustaches. Choose from several classic trendsetters - you simply cannot make a bad lip choice. Applies easily by removing the transparent tape and affixing to skin. Remove gently.


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PRLog - Feb. 12, 2014 - Poetic Cases & Accessories, purveyor of affordable, cutting-edge cases and accessories for Smartphones and tablets that are renowned for fashion-forward style and superb protection, is releasing an array of cases in alignment with this week's launch of Samsung's much-awaited series of tablets: Samsung Galaxy Tab Pro 8.4, Samsung Galaxy Tab Pro 10.1 and Samsung Galaxy Note Pro 12.2.
Poetic Cases
Poetic will have the following series available for each of the new Samsung tablets: SlimBook, SlimLine and GraphGrip. These superb cases will be available for Pre-Order for March 31 at poeticcases.com.
The sleek, ultra lightweight SlimBook is so slim, you'll forget there is a case on your Samsung tablet. Made from smooth and elegant PU leather, the SlimBook provides sturdy protection without adding bulk. Designed specifically for the Samsung Galaxy Tab Pro tablets, the SlimBook features perfect port cutouts for the device's headphones, charging cable, and speakers.
The SlimLine offers a sturdy folder design that protects the screen and the back of the device. The microfiber interior lining helps prevent scratches and damage from dust, as well as anti-slip protection when rolling back the cover in multiple view positions. The magnetic cover protects your screen on the go and offer a sleep/wake function upon opening and closing. This shock-resistant case is made with environmentally friendly, high-quality, flexible PU and has easy snap on/snap off functions.
Made of durable impact-resistant silicone, the GraphGrip series for the new Samsung tablets offers excellent grip thanks to stylish and playful grooved square pattern throughout—it doesn't just look good, it gets the job done. The GraphGrip's snug fit features precision cutouts for easy access to all ports, buttons and speakers.
For more information, visit poeticcases.com.
About Poetic
Globally renowned by savvy smartphone and tablet case enthusiasts, Poetic has quickly become the go-to source for cases that are both innovative and high-quality at the greatest value. A growing force in the tech accessory industry, Poetic provides shoppers a wide selection of today's most cutting-edge cases and accessories.
Jacquelyn Lucchesi
Jacquelyn Lucchesi


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You will enjoy:
Bursting with natural fruit extracts and moisturisers, the juicy treatment includes express cuticle work, moisturisers and nail paint for the girl on the go.
Online bookings let you pick date/time for your visit.
If you purchase an eVoucher you will need to book with the venue directly.
Our old Wahanda Gift Vouchers can still be redeemed at over 3,000 locations. Use them to pay as you would with cash. Please note, the Wahanda Gift Voucher's successor – the Wahanda Gift Card – can only be used for online purchases.


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Are you Xi Wang?Claim your profile
Publications (45)108.15 Total impact
Article: Quantitative evaluation of maxillary interradicular bone with cone-beam computed tomography for bicortical placement of orthodontic mini-implants[Show abstract] [Hide abstract]
ABSTRACT: The purpose of this study was to propose a protocol for safe bicortical placement of mini-implants by measuring the interradicular spaces of the maxillary teeth and the bone quality. Cone-beam computed tomography data were obtained from 50 adults. Three-dimensional reconstructions and measurements were made with SimplantPro software (Materialise, Leuven, Belgium). For each interradicular site, the bone thicknesses and interradicular distances at the planes 1.5, 3, 6, and 9 mm above the cementoenamel junction were measured. Standard bone units were defined to evaluate the influences of bone density and the different placement patterns on the stability of the mini-implants. The safe interradicular sites in the maxilla for bicortical placement of 1.5-mm-diameter mini-implants were in all planes between the first and second premolars, and between the second premolar and the first molar. The safe palatal sites were between the first and second molars, and the safe labial sites of the 9-mm plane were between the central incisors, and between the lateral incisor and the canine. The safe buccal sites of the 6- and 9-mm planes were between the first and second molars, and the safe buccal sites of the 3-, 6-, and 9-mm planes were between the canine and the first premolar. Most bone thicknesses were from 8 to 12 mm. The optimal placement angle between the second premolar and the first molar was 58°. Bicortical placement could have more standard bone units than unicortical placement in the maxilla. Bicortical placement would be more stable in the maxilla. For the site between the molars, special care should be taken at a plane higher than 6 mm to prevent maxillary sinus penetration. The most favorable interradicular area in the maxilla was between the second premolar and the first molar. Copyright © 2015 American Association of Orthodontists. Published by Elsevier Inc. All rights reserved.American journal of orthodontics and dentofacial orthopedics: official publication of the American Association of Orthodontists, its constituent societies, and the American Board of Orthodontics 06/2015; 147(6). DOI:10.1016/j.ajodo.2015.02.018 · 1.44 Impact Factor
Article: Impact of estrogen-related receptor a on the biological characteristics of rat mandibular condylar chondrocytes[Show abstract] [Hide abstract]
ABSTRACT: It is well‑known that estrogen-related receptor α (ERRα) affects numerous metabolic pathways and biological functions in the body, although the function of ERRα in the mandibular condylar chondrocytes (MCCs) of the temporomandibular joint remains unclear. The aim of the present study was to investigate the effect of ERRα on the biological characteristics of MCCs in female rats. Immunofluorescent staining was used to observe the expression level and distribution of ERRα in MCCs and tissues. Quantitative polymerase chain reaction (qPCR) was performed to detect the impact of estrogen intervention on the biological characteristics of female rat MCCs and ERRα expression levels. Liposome transfection and XCT‑790 were used to overexpress and inhibit ERRα expression, respectively, and then qPCR was performed to detect changes in the biological characteristics of MCCs. ERRα expression was detected in the nucleus and cytoplasm of rat MCCs. 17‑β estradiol (E2) (10‑8 M) increased the mRNA and protein expression levels of ERRα, Sox9, GDF‑5 and aromatase during in vitro MCC cultivation. In addition, E2 affected MCC proliferation through the regulation of ERRα expression levels. Overexpression of ERRα positively regulated the mRNA and protein expression levels of Sox9 and GDF‑5, but did not exhibit a significant effect on the mRNA and protein expression levels of aromatase and Col2a1. In conclusion, ERRα exhibited an important regulatory role in the proliferation and differentiation of female Sprague‑Dawley rat MCCs in vitro through regulating Sox9 and GDF-5.Molecular Medicine Reports 05/2014; 10(1). DOI:10.3892/mmr.2014.2210 · 1.48 Impact Factor
Article: [Bone marrow-derived mesenchymal stem cells from estrogen deficiency induced osteoporosis mice regulate T cell migration and apoptosis through expressing MCP-1].[Show abstract] [Hide abstract]
ABSTRACT: Objective To reveal the role of bone marrow-derived mesenchymal stem cells (BMSCs) in the development of osteoporosis by comparing the differences in monocyte chemoattractant protein-1 (MCP-1) expression and T cells' migration and apoptosis induced by BMSCs from ovariectomy (OVX) group and sham group. Methods OVX was performed on C57BL/6 mice to establish the animal models of osteoporosis. Osteoporosis was confirmed by micro-CT. The expression of MCP-1 between OVX group and sham group was examined by ELISA; after exogenous estrogen of different concentrations were given to stimulate BMSCs from OVX group, the expression of MCP-1 was observed again by ELISA. Through co-culturing of BMSCs and T cells, the change of T cells' migration and apoptosis capacity induced by BMSCs was compared between OVX group and sham group. And also, we observed the effects of exogenous estrogen of different concentrations on the T cells' migration and apoptosis capacity. Results In animal models of osteoporosis induced by estrogen deficiency, BMSCs had a declined inducing effect on the capacity of T cell migration and apoptosis and expressed a decreased level of migration-related gene MCP-1. After the stimulation of estrogen of certain concentration, the declining tendency was revised to some extent. Conclusion Through expressing MCP-1, BMSCs could regulate the capacity of T cell migration and apoptosis, thus leading to the development of osteoporosis.Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 01/2014; 30(1):19-22.
Article: [Role of bone marrow-derived mesenchymal stem cells in treating estrogen deficiency induced osteoporosis].[Show abstract] [Hide abstract]
ABSTRACT: Objective To investigate the therapeutic effects of bone marrow-derived mesenchymal stem cells (BMSCs) from C57BL/6 mice on estrogen deficiency induced osteoporosis. Methods Mouse models of estrogen deficiency induced osteoporosis were set up through ovariectomy (OVX) operation and sham operation group was set up as controls. BMSCs were injected via caudal veins. Micro-CT scanning of the femurs was conducted to detect the therapeutic effects of BMSCs. ELISA was used to test the expression level of TNF-α in serum before and after the injection of BMSCs. In the meantime, T cell apoptosis was also tested by flow cytometry combined with FITC-annexin V/7-amino actimycin D staining. Results Compared with the sham operation group, the trabecular volume (BV/TV), bone mineral density (BMD) and trabecular number (Tb.N/mm) of osteoporosis mice set up by OVX were reduced significantly, and serum TNF-α was up-regulated a little. After the injection of BMSCs, the BV/TV, Tb.Th, Tb.N and T cell apoptosis in the osteoporosis mice increased, and the level of TNF-α decreased. Conclusion With the ability of immunoregulation, BMSCs might play a critical role in treating estrogen deficiency induced osteoporosis.Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 12/2013; 29(12):1267-71.
Article: Coculture of peripheral blood CD34+ cell and mesenchymal stem cell sheets increase the formation of bone in calvarial critical-size defects in rabbits[Show abstract] [Hide abstract]
ABSTRACT: The reconstruction of large bony defects remains a clinical challenge, and angiogenesis and neovascularisation are being given more attention in bone tissue engineering. In this study we cocultured peripheral blood CD34+ cells (PB-CD34+ cells), an endothelial progenitor cell/haematopoietic stem cell-enriched population, with bone marrow-derived mesenchymal stem cells (MSC) to investigate their potential for bony regeneration. Cocultured cells showed better osteogenic differentiation than MSC alone in vitro. The cocultured cells and MSC sheets were also composited with hydroxyapatite and implanted in calvarial critical-size defects in rabbits. The rabbits were killed before microcomputed tomographic (MicroCT) and histological analysis. The results showed that cocultured cell composites had promoted bony regeneration more efficiently by 8 weeks after implantation. Our results indicate that the coculture of PB-CD34+ cells and MSC increases bony regeneration in calvarial critical-size defects in rabbits, and provide a new promising therapeutic strategy to aid skeletal healing.British Journal of Oral and Maxillofacial Surgery 11/2013; 52(2). DOI:10.1016/j.bjoms.2013.10.004 · 1.13 Impact Factor
Article: [Role of bone marrow-derived mesenchymal stem cells in treating colitis through Fas/FasL-mediated immune regulation.][Show abstract] [Hide abstract]
ABSTRACT: Objective To investigate the difference in the therapeutic effects of bone marrow-derived mesenchymal stem cells (BMSCs) of mice from ovariectomy (OVX) group and sham group in treating colitis, and then further study the differences of Fas/FasL expression and downstream T cell migration and apoptosis between the two groups. Methods The osteoporosis animal models were set up by ovariectomy in C57BL6 mice. Meanwhile, 3% dextran sulfate sodium (DSS) was administered for inducing colitis. We compared the therapeutic effects of BMSCs from OVX and sham groups in treating colitis, in addition, detected the expression of Fas/FasL in BMSCsby means of RT-PCR and Western blotting. The ability of BMSCs from the two groups of inducing T cell migaration and apoptosis was also detected. Results Compared with the sham group, BMSCs from OVX mice expressed a lower level of Fas/FasL and displayed a decreased ability of inducing T cell migration and apoptosis, thus leading to an inferior therapeutic effect in treating colitis in animal models. Conclusion Fas/FasL expression of BMSCs from the OVX mice is down regulated, thus leading to a decrease of the migration and apoptosis for T cells from mouse colitis.Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology 10/2013; 29(10):1028-1031.
Article: Three-dimensional facial anthropometry of unilateral cleft lip infants with a structured light scanning system[Show abstract] [Hide abstract]
ABSTRACT: BACKGROUND: Cleft lip in the presence or absence of a cleft palate is a major public health problem. However, few studies have been published concerning the soft-tissue morphology of cleft lip infants. Currently, obtaining reliable three-dimensional (3D) surface models of infants remains a challenge. The aim of this study was to investigate a new way of capturing 3D images of cleft lip infants using a structured light scanning system. In addition, the accuracy and precision of the acquired facial 3D data were validated and compared with direct measurements. STUDY DESIGN: Ten unilateral cleft lip patients were enrolled in the study. Briefly, 3D facial images of the patients were acquired using a 3D scanner device before and after the surgery. Fourteen items were measured by direct anthropometry and 3D image software. The accuracy and precision of the 3D system were assessed by comparative analysis. RESULTS: The anthropometric data obtained using the 3D method were in agreement with the direct anthropometry measurements. All data calculated by the software were 'highly reliable' or 'reliable', as defined in the literature. The localisation of four landmarks was not consistent in repeated experiments of inter-observer reliability in preoperative images (P < 0.05), while the intra-observer reliability in both pre- and postoperative images was good (P > 0.05). CONCLUSIONS: The structured light scanning system is proven to be a non-invasive, accurate and precise method in cleft lip anthropometry.Journal of Plastic Reconstructive & Aesthetic Surgery 05/2013; 66(8). DOI:10.1016/j.bjps.2013.04.007 · 1.47 Impact Factor
Article: Estrogen-related receptor α is involved in the osteogenic differentiation of mesenchymal stem cells isolated from human periodontal ligaments.[Show abstract] [Hide abstract]
ABSTRACT: Recently, it has been reported that the orphan nuclear receptor estrogen-related receptor α (ERRα) is involved in the osteogenic differentiation of mesenchymal stem cells (MSCs). Moreover, ERRα has been identified as a novel therapeutic target for treating osteoporosis and other bone diseases. Human periodontal ligament tissue-derived mesenchymal stem cells (hPDLSCs) have recently been used in stem cell-mediated therapies because of their multipotency, particularly toward osteogenic differentiation. However, it is still unclear whether ERRα can regulate the osteogenic differentiation of hPDLSCs. In the present study, we investigated the role of ERRα in the osteogenic differentiation of hPDLSCs in vitro. We isolated hPDLSCs and confirmed their capacity for multipotent differentiation. Furthermore, we examined ERRα expression in hPDLSCs by RT-PCR and immunocytochemistry. We found that the expression of ERRα mRNA was significantly increased during the late stage of osteogenic differentiation of hPDLSCs. Moreover, transfection of recombinant lentiviral-mediated miRNA targeting ERRα significantly suppressed ALP activity, mineralization capacity, and the mRNA expression of osteogenesis-related genes (ALP, OCN, RUNX2 and OPN) in hPDLSCs. Our results indicate that ERRα may promote the osteogenic differentiation of hPDLSCs in vitro.International Journal of Molecular Medicine 03/2013; 31(5). DOI:10.3892/ijmm.2013.1305 · 1.88 Impact Factor
Article: AEG-1 Overexpression is Essential for Maintenance of Malignant State in Human AML Cells via Up-regulation of Akt1 Mediated by AURKA Activation.[Show abstract] [Hide abstract]
ABSTRACT: Acute myeloid leukemia (AML) remains highly fatal, highlighting the need for improved understanding of signal pathways that can lead to the development of new therapeutic regimens targeting common molecular pathways shared across different AML subtypes. Here we demonstrate that Astrocyte elevated gene-1 (AEG-1) is one of such pathways, involving in cell cycle and apoptosis regulation and contributing to enhanced proliferation and chemoresistance in HL-60 and U937 AML cells. The pleiotropic effects of AEG-1 on AML were found to correlate with two novel target genes, Aurora kinase A (AURKA) and Akt1. Down-regulation of AEG-1 by short-hairpin RNA (shRNA) could not only decrease AURKA expression both on mRNA and protein levels but also decrease the levels of pAkt473 and pAkt308(the active forms of phosphorylated Akt), similar effect as using AURKA inhibitor Tozasertib (VX680). Furthermore, the AEG-1 shRNA-induced malignant phenotype changes could be mitigated by forced overexpression of AURKA through increased Akt1 activation and phosphorylation in AML cells. On the other hand, although exogenous expression of AEG-1 could increase both AURKA and Akt expression levels the simultaneous use of AURKA inhibitor Tozasertib blocked AEG-1's role of up-regulation of Akt expression in ECV304 cells, suggesting that AURKA might be a key mediator of AEG-1 in regulating Akt activation, and a key effector of AEG-1 in maintaining the malignant state of AML. Moreover, knockdown AEG-1 expression also changed the expression levels of PTEN, survivin and stathmin, the genes that have been reported to be involved in the development of several other malignant tumors. Our results provide evidence for AEG-1's carcinogenesis role in AML and reveal a novel functional link between AEG-1 and AURKA on Akt1 activation. AEG-1 can be an important candidate as a drug design target within AURKA signal pathway for more specific killing of AML cells while sparing normal cells.Cellular Signalling 03/2013; DOI:10.1016/j.cellsig.2013.03.001 · 4.47 Impact Factor
Article: TNF-α suppresses the mesenchymal stem cell osteogenesis promoter miR-21 in estrogen deficiency-induced osteoporosis.[Show abstract] [Hide abstract]
ABSTRACT: Inflammatory cytokines, especially TNF-α, have been shown to inhibit osteogenic differentiation of mesenchymal stem cells (MSCs) and bone formation in estrogen deficiency-induced osteoporosis, but the mechanism responsible remains poorly understood. MicroRNAs (miRNAs) have been shown to regulate MSC differentiation. Here, we identified a novel mechanism that TNF-α suppressing the functional axis of a key miRNA(miR-21) contributes to estrogen deficiency-induced osteoporosis. In this study, we screened a key miRNA (miR-21) that was significantly down-regulated in MSCs derived from estrogen deficiency-induced osteoporosis. The miR-21 was suppressed by TNF-α during the osteogenesis of MSCs. Furthermore, miR-21 was confirmed to promote the osteoblast differentiation of MSCs by repressing Spry1, which can negatively regulate the osteogenic differentiation of MSCs. Up-regulating miR-21 partially rescued TNF-α-impaired osteogenesis of MSCs. Blocking TNF-α ameliorated the inflammatory environment and significantly enhanced bone formation with increased miR-21 expression and suppressed Spry1 expression in ovariectomized (OVX) mice. Our results revealed a novel function for miR-21 and suggested that suppressed miR-21 may contribute to impaired bone formation by elevated TNF-α in estrogen deficiency-induced osteoporosis. This study may indicate a molecular basis for novel therapeutic strategies against osteoporosis and other inflammatory bone diseases. © 2012 American Society for Bone and Mineral Research.Journal of bone and mineral research: the official journal of the American Society for Bone and Mineral Research 03/2013; 28(3). DOI:10.1002/jbmr.1798 · 6.59 Impact Factor
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ABSTRACT: Abstract miR-34a was identified as one of the downregulated microRNAs (miRNAs) in human lung cancer. However, the precise biological role of miR-34a in p53 deficient lung cancer cell lines remains largely elusive. In the present study, we aimed to identify the role of miR-34a in the regulation of lung cancer cell proliferation. Using quantitative RT-PCR analysis, we found that miR-34a was highly upregulated in the p53 wild-type A549 human lung cancer cell line when treated with the DNA damaging agent adriamycin (ADR), but not in the SBC-5 cells harboring mutated p53. Transient introduction of miR-34a into A549 and SBC-5 cell lines caused complete suppression of cell proliferation and induced the cell cycle arrested at the G(1) phase. When we knockdown the miR-34a downstream target-Sitr1- using the small-interfering RNA, there was also a cell growth inhibition in both cell lines though not as much as miR-34a did. Moreover, we demonstrated that pretransfection of miR-34a could increase the sensitivity of both lung cancer cell lines to cisplatin (DDP), and this could be reverted by the miR-34a inhibitor. Moreover, when cells pretreated with siR-Sirt1, they are more sensitive to DDP than the control pretreated cells as well. We thus hypothesize the miR-34a/Sirt1 cascade involved with p53-independent functions. Overall, in this study, we found the proliferation inhibition function of miR-34a in vitro in lung cancer cell lines is p53 independent, and also demonstrated the combination therapeutic potential of miR-34a and DDP in lung cancer cell lines.Cancer Biotherapy & Radiopharmaceuticals 10/2012; DOI:10.1089/cbr.2012.1218 · 1.38 Impact Factor
Article: Molecular targeting regulation of proliferation and differentiation of the bone marrow-derived mesenchymal stem cells or mesenchymal stromal cells.[Show abstract] [Hide abstract]
ABSTRACT: The bone marrow-derived mesenchymal stem cells or mesenchymal stromal cells (MSCs), with pluripotent differentiation capacity, present an ideal source for cell transplantation or tissue engineering therapies, but exact understanding of regulating mechanism underling MSC proliferation and differentiation remains a critical issue in securing their safe and efficient clinical application. This review outlines current knowledge regarding MSC cell surface biomarkers and molecular mechanisms of MSC differentiation and proliferation with emphasis on Wnt/β-catenin signaling, Notch signaling pathway, bone morphogenesis proteins and various growth factors functioning in regulation of differentiation and proliferation of MSCs. Possible relation of oncogene and immunosuppressive activities of MSCs with tumorigenicity or tumor generation is also addressed for safe translational clinical application. Fast increase of MSC knowledge and techniques has led to some successful clinical trials and helped devising new tissue engineering therapies for bone and cartilage diseases that severely afflict human health. Production of adult MSC-derived functional neurons can further extend their therapeutic application in nerve injury and neurodegenerative diseases. It is promising that MSCs shall overcome ethical and immunorejection problems appeared in human embryonic stem cells, and specific molecular targeting manipulation may result in practical MSC therapy for personalized treatment of various diseases in the regeneration medicine.Current drug targets 04/2012; 13(4):561-71. · 3.60 Impact Factor
Article: Comparative characterization of stem cells from human exfoliated deciduous teeth and dental pulp stem cells[Show abstract] [Hide abstract]
ABSTRACT: This study focused on the characterization of stem cells from human exfoliated deciduous teeth (SHED) in comparison with dental pulp stem cells (DPSCs) to certify SHED as a key element in tissue engineering. In the present study, SHED and DPSCs were assayed for their cell surface antigens and proliferation by measuring the cell cycles, growth rates, Ki67-positive efficiencies, and colony-forming units (CFUs). The evaluation of multi-differentiation was performed using alizarin red and oil red O and real-time PCR in vitro. The mineralization capability of the cells was examined in vivo by implanting with ceramic bovine bone (CBB) into subcutaneous of immunocompromised mice for 8weeks. A three-dimensional pellet cultivation system is proposed for SHED and DPSCs to recreate the biological microenvironment that is similar to that of a regenerative milieu. SHED showed a higher proliferation rate and differentiation capability in comparison with DPSCs in vitro, and the results of the in vivo transplantation suggest that SHED have a higher capability of mineralization than the DPSCs. The mRNA expression levels of inflammatory cytokines, including matrix metalloproteinase-1 (MMP1), tissue inhibitors of metalloproteinase-1 (TIMP1), matrix metalloproteinase-2 (MMP2), tissue inhibitors of metalloproteinase-2 (TIMP2) and interleukin-6 (IL-6) were higher in SHED than that in DPSCs. In addition, the expression levels of Col I and proliferating cell nuclear antigen (PCNA) in SHED sheets were significantly higher than those in DPSCs sheets. This study systematically demonstrated the differences in the growth and differentiation characteristics between SHED and DPSCs. Consequently, SHED may represent a suitable, accessible and potential alternative source for regenerative medicine and therapeutic applications.Archives of oral biology 03/2012; 57(9):1231-40. DOI:10.1016/j.archoralbio.2012.02.014 · 1.88 Impact Factor
Article: Brain-derived neurotrophic factor stimulates proliferation and differentiation of neural stem cells, possibly by triggering the Wnt/β-catenin signaling pathway[Show abstract] [Hide abstract]
ABSTRACT: Brain-derived neurotrophic factor (BDNF) has critical functions in promoting survival, expansion, and differentiation of neural stem cells (NSCs), but its downstream regulation mechanism is still not fully understood. The role of BDNF in proliferation and differentiation of NSCs through Wnt/β-catenin signaling was studied via cell culture of cortical NSCs, Western blotting, immunocytochemistry, and TOPgal (Wnt reporter) analysis in mice. First, BDNF stimulated NSC proliferation dose dependently in cultured neurospheres that exhibited BrdU incorporation and neuronal and glial differentiation abilities. Second, BDNF effectively enhanced cell commitment to neuronal and oligodendrocytic fates, as indicated by increased differentiation marker Tuj-1 (neuronal marker), CNPase (oligodendrocyte marker), and neuronal process extension. Third, BDNF upregulated expression of Wnt/β-catenin signaling (Wnt1 and free β-catenin) molecules. Moreover, these promoting effects were significantly inhibited by application of IWR1, a Wnt signaling-specific blocker in culture. The TOPgal mouse experiment further confirmed BDNF-triggered Wnt signaling activation by β-gal labeling. Finally, an MEK inhibition experiment showed a mediating role of the microtubule-associated protein kinase pathway in BDNF-triggered Wnt/β-catenin signaling cascades. This study overall has revealed that BDNF might contribute to proliferation and neuronal and oligodendrocytic differentiation of NSCs in vitro, most possibly by triggering the Wnt/β-catenin signaling pathway. Nevertheless, determining the exact cross-talk points at which BDNF might stimulate Wnt/β-catenin signaling pathway in NSC activity requires further investigation. © 2012 Wiley Periodicals, Inc.Journal of Neuroscience Research 01/2012; 91(1). DOI:10.1002/jnr.23138 · 2.73 Impact Factor
Article: Molecular manipulation targeting regulation of dopaminergic differentiation and proliferation of neural stem cells or pluripotent stem cells.[Show abstract] [Hide abstract]
ABSTRACT: Parkinson's disease (PD) is a severe deliberating neurological disease caused by progressive degenerative death of dopaminergic neurons in the substantia nigra of midbrain. While cell replacement strategy by transplantation of neural stem cells and inducement of dopaminergic neurons is recommended for the treatment of PD, understanding the differentiation mechanism and controlled proliferation of grafted stem cells remain major concerns in their clinical application. Here we review recent studies on molecular signaling pathways in regulation of dopaminergic differentiation and proliferation of stem cells, particularly Wnt/beta-catenin signaling in stimulating formation of the dopaminergic phenotype, Notch signaling in inhibiting stem cell differentiation, and Sonic hedgehog functioning in neural stem cell proliferation and neuronal cell production. Activation of oncogenes involved in uncontrolled proliferation or tumorigenicity of stem cells is also discussed. It is proposed that a selective molecular manipulation targeting strategy will greatly benefit cell replacement therapy for PD by effectively promoting dopaminergic neuronal cell generation and reducing risk of tumorigenicity of in vivo stem cell applications.CNS & neurological disorders drug targets 06/2011; 10(4):517-28. DOI:10.2174/187152711795563912 · 2.70 Impact Factor
Article: Induction of HIV neutralizing antibodies against the MPER of the HIV envelope protein by HA/gp41 chimeric protein-based DNA and VLP vaccines.[Show abstract] [Hide abstract]
ABSTRACT: Several conserved neutralizing epitopes have been identified in the HIV Env protein and among these, the MPER of gp41 has received great attention and is widely recognized as a promising target. However, little success has been achieved in eliciting MPER-specific HIV neutralizing antibodies by a number of different vaccine strategies. We investigated the ability of HA/gp41 chimeric protein-based vaccines, which were designed to enhance the exposure of the MPER in its native conformation, to induce MPER-specific HIV neutralizing antibodies. In characterization of the HA/gp41 chimeric protein, we found that by mutating an unpaired Cys residue (Cys-14) in its HA1 subunit to a Ser residue, the modified chimeric protein HA-C14S/gp41 showed increased reactivity to a conformation-sensitive monoclonal antibody against HA and formed more stable trimers in VLPs. On the other hand, HA-C14S/gp41 and HA/gp41 chimeric proteins expressed on the cell surfaces exhibited similar reactivity to monoclonal antibodies 2F5 and 4E10. Immunization of guinea pigs using the HA-C14S/gp41 DNA or VLP vaccines induced antibodies against the HIV gp41 as well as to a peptide corresponding to a segment of MPER at higher levels than immunization by standard HIV VLPs. Further, sera from vaccinated guinea pigs were found to exhibit HIV neutralizing activities. Moreover, sera from guinea pigs vaccinated by HA-C14S/gp41 DNA and VLP vaccines but not the standard HIV VLPs, were found to neutralize HIV pseudovirions containing a SIV-4E10 chimeric Env protein. The virus neutralization could be blocked by a MPER-specific peptide, thus demonstrating induction of MPER-specific HIV neutralizing antibodies by this novel vaccine strategy. These results show that induction of MPER-specific HIV neutralizing antibodies can be achieved through a rationally designed vaccine strategy.PLoS ONE 05/2011; 6(5):e14813. DOI:10.1371/journal.pone.0014813 · 3.53 Impact Factor
Article: The TrkB-Positive Dopaminergic Neurons are Less Sensitive to MPTP Insult in the Substantia Nigra of Adult C57/BL Mice[Show abstract] [Hide abstract]
ABSTRACT: Tyrosine kinase receptors TrkB and TrkC mediate neuroprotective effects of the brain-derived neurotrophic factor (BDNF) and neurotrophins in the dopaminergic nigro-striatal system, but it is obscure about their responses or expression changes in the injured substantia nigra under Parkinson's disease. In present study, immunofluorescence, Fluoro-Jade staining and laser scanning confocal microscopy were applied to investigate distribution and changes of TrkB and TrkC in the dopamine neurons of the substantia nigra by comparison of control and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) mouse model. It revealed that TrkB and TrkC-immunoreactivities were substantially localized in cytoplasm and cell membrane of the substantia nigra neurons of control adults. While neurons double-labeled with tyrosine hydroxylase (TH)/TrkB, or TH/TrkC were distributed in a large numbers in the substantia nigra of controls, they apparently went down at 36.2-65.7% of normal level, respectively following MPTP insult. In MPTP model, cell apoptosis or degeneration of nigral neurons were confirmed by caspase-3 and Fluoro-Jade staining. More interestingly, TH/TrkB-positive neurons survived more in cell numbers in comparison with that of TH/TrkC-positive ones in the MPTP model. This study has indicated that TrkB-containing dopamine neurons are less sensitive in the substantia nigra of MPTP mouse model, suggesting that specific organization of Trks may be involved in neuronal vulnerability to MPTP insult, and BDNF-TrkB signaling may play more important role in protecting dopamine neurons and exhibit therapeutic potential for Parkinson's disease.Neurochemical Research 05/2011; 36(10):1759-66. DOI:10.1007/s11064-011-0491-5 · 2.55 Impact Factor
Article: Synergistic tumor growth-inhibitory effect of the prostate-specific antigen-activated fusion peptide BSD352 for prostate cancer therapy[Show abstract] [Hide abstract]
ABSTRACT: Prostate-specific antigen (PSA), a serine protease, is a promising target for the development of prodrugs in prostate cancer treatment. In this study, we designed a novel fusion peptide, BSD352, containing three functional domains: a protein transduction domain from HIV transactivating regulatory protein (TAT) followed by the BH3 domain of the p53 upregulated modulator of apoptosis (TAT-BH3), an anti-vascular endothelial growth factor peptide (SP5.2), and an anti-basic fibroblast growth factor peptide (DG2). These different domains in BSD352 were linked together by a linker sequence corresponding to a PSA hydrolytic substrate peptide. The BSD352 fusion peptide could be selectively cleaved by PSA in PSA-producing LNCaP prostate cancer cells. Furthermore, the BSD352 fusion peptide was efficiently transduced into tumor cells both in vitro and in vivo, and the BH3 domain was found to induce tumor cell apoptosis by elevating the expression of Bax, cytochrome C release, and caspase-9 cleavage. Moreover, the SP5.2 and DG2 domains in the BSD352 fusion peptide also exhibited in-vitro endothelial cell growth inhibition and in-vivo antiangiogenic activities. Direct injection of BSD352 into an established LNCaP xenograft tumor in mice inhibited tumor growth, whereas a synergistic effect was observed with the combined use of wild-type BH3, SP5.2, and DG2 functional domains. These results suggest that BSD352 could be beneficial for the treatment of accessible prostate tumors and may provide a complementary strategy for prostate cancer therapy.Anti-cancer drugs 03/2011; 22(3):213-22. DOI:10.1097/CAD.0b013e3283401f4d · 1.89 Impact Factor
Article: Clinical, pathological and genetic evaluations of Chinese patients with autosomal-dominant hypophosphatasia[Show abstract] [Hide abstract]
ABSTRACT: Hypophosphatasia (HPP) is an inherited disorder characterised by defective bone and tooth mineralisation and deficient serum and bone alkaline phosphatase activity, and it results from mutations in alkaline phosphatase (ALPL) encoding tissue-nonspecific alkaline phosphatase (TNAP). The objective of the present work was to explore the correlations between genotype and phenotype in a Chinese family affected by autosomal-dominant HPP. We examined all individuals of a HPP family by clinical and radiographic examinations as well as laboratory assays. Furthermore, a prematurely exfoliated tooth was observed histopathologically. Based on the clinical and pathological manifestations, the causative gene ALPL was selected for further analysis and screened for mutations. The proband presented the characteristic clinical features of childhood HPP such as rachitic skeletal changes, early loss of primary teeth, and short root anomalies of the permanent teeth. Histopathological evaluation of a tooth revealed a "shell" structure, severe mineralisation defects of dentin, and an absence of cementum. The patient's mother and grandfather were clinically diagnosed with adult HPP. The family showed autosomal-dominant moderate hypophosphatasia. DNA sequencing and analysis revealed a novel missense mutation (c.251A>T) in exon4 of ALPL. This mutation (p.E84V) is located in the secondary structure of TNAP's homodimer interface, and it was predicted to have a dominant negative effect. Our findings suggest the missense transversion (c.251A>T, p.E84V) should be responsible for the HPP phenotype in this Chinese family.Archives of oral biology 12/2010; 55(12):1017-23. DOI:10.1016/j.archoralbio.2010.08.003 · 1.88 Impact Factor
Article: p21WAF1/CIP1 gene transcriptional activation exerts cell growth inhibition and enhances chemosensitivity to cisplatin in lung carcinoma cell[Show abstract] [Hide abstract]
ABSTRACT: Non-small-cell lung carcinomas (NSCLCs) exhibit poor prognosis and are usually resistant to conventional chemotherapy. Absence of p21WAF1/CIP1, a cyclin-dependent kinase (cdk) inhibitor, has been linked to drug resistance in many in vitro cellular models. RNA activation (RNAa) is a transcriptional activation phenomena guided by double-strand RNA (dsRNA) targeting promoter region of target gene. In this study, we explored the effect of up-regulation of p21 gene expression on drug-resistance in A549 non-small-cell lung carcinoma cells by transfecting the dsRNA targeting the promoter region of p21 into A549 cells. Enhanced p21 expression was observed in A549 cells after transfection of dsRNA, which was correlated with a significant growth inhibition and enhancement of chemosensitivity to cisplatin in A549 cells in vitro. Moreover, in vivo experiment showed that saRNA targeting the promoter region of p21 could significantly inhibit A549 xenograft tumor growth. These results indicate that p21 plays a role in lung cancer drug-resistance process. In addition, this study also provides evidence for the usage of saRNA as a therapeutic option for up-regulating lower-expression genes in lung cancer.BMC Cancer 11/2010; 10:632. DOI:10.1186/1471-2407-10-632 · 3.32 Impact Factor
Top Journals
Fourth Military Medical University
Xi'an, Liaoning, China
- • Department of Orthodontics
- • Department of Hematology
- • Institute of Neurosciences
Zhengzhou UniversityCheng, Henan Sheng, China
Chongqing Medical UniversityCh'ung-ch'ing-shih, Chongqing Shi, China


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Spring 2015 | Volume 21, No. 3
The Spring issue of XRDS features contributions from the who's who of cryptography. Within are a range of articles from the early beginnings of cryptography through present-day research. Discover modern cryptographic techniques like searchable encryption, read interviews with leading researchers in the field, and enjoy a wonderful look back at Bletchley Park, home of Colossus (the world's first electronic digital computer)


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Last summer, the finance minister put new mortgage rules into place in an attempt to engineer a slowdown.
The hidden story embedded in the Chinese economic "slowdown" is that investment-led growth is plunging.
What "subtracted ... 2.6 percent from growth" was reduced government spending, along with a slowdown in filling inventories.


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Food Encyclopedia
Browse Alphabetically
Pronunciation: [KWAYL]
The American quail is not related to the European quail, a migratory game bird belonging to the partridge family. But when colonists discovered birds that resembled the European version they called them by the same name. American quail are known by various names depending on the regionbobwhite in the East, partridge in the South, quail in the North and blue quail in the Southwest. Other notable members of this family are California quail, mountain quail and Montezuma quail. American quail nest on the ground and are not migratoryin fact, they'd rather walk than fly. They're very social and travel in small groups called coveys. The meat of the American quail is white and delicately flavored. In general, they should be cooked like other game birdsyoung birds can be roasted, broiled or fried and older fowl should be cooked with moist heat. Most of the quail marketed today are raised on game bird farms. Fresh quail can be ordered through specialty butchers, who might also carry frozen quail.
From The Food Lover's Companion, Fourth edition by Sharon Tyler Herbst and Ron Herbst. Copyright © 2007, 2001, 1995, 1990 by Barron's Educational Series, Inc.
Related Recipes From Food Network
- Shrimp and Andouille Stuffed Quail with Spicy White Wine Sauce
- Grilled Quail with Pomegranate Molasses and Horseradish Glaze with Spicy Walnuts and Tangerine Vinaigrette
- Quail Stuffed with Sausage
- Grilled Quail Breast Wrapped in Prosciutto with Black Trumpet Mushrooms, Baby Leeks, Pea Tendrils and Saffron Noodles
- B.B.Q. Quail


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Tobias Buer
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Personal Details
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Location: Bremen, Germany
Homepage: http://www.wiwi.uni-bremen.de/
Postal: Postfach 33 04 40, 28334 Bremen
Handle: RePEc:edi:fwbrede (more details at EDIRC)
Homepage: http://www.wiwi.uni-bremen.de/
Postal: Postfach 33 04 40, 28334 Bremen
Handle: RePEc:edi:fwbrede (more details at EDIRC)
- Buer, Tobias & Kopfer, Herbert, 2012. "A Pareto-metaheuristic for a bi-objective winner determination problem in a combinatorial reverse auction," MPRA Paper 36062, University Library of Munich, Germany.
- Tobias Buer & Giselher Pankratz, 2010. "GRASP with Hybrid Path Relinking for Bi-Objective Winner Determination in Combinatorial Transportation Auctions," BuR - Business Research, German Academic Association for Business Research, vol. 3(2), pages 192-213, November.
1 paper by this author was announced in NEP, and specifically in the following field reports (number of papers):
- NEP-CMP: Computational Economics (1) 2012-02-01. Author is listed
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The Quotable Rogue: The Ideals of Sarah Palin in Her Own WordsPaperback
List price $14.02
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- Publisher: Nelson Current
- Format: Paperback | 192 pages
- Dimensions: 137mm x 211mm x 15mm | 249g
- Publication date: 28 June 2011
- Publication City/Country: Nashville, TN
- Sales rank: 1,915,922
Product description
When Sarah Palin says it, all of America hears it. In the few years since Americans met Sarah Palin, her every word has been chronicled, analyzed, celebrated, and yes, infamously disparaged.Regardless of your party affiliation, it's impossible to deny her influence on American politics and the dialogue her ideas have generated. Author Matt Lewis has gathered some of Palin's most memorable remarks, reminding us that whether you consider her a rogue or a patriot, her words reveal a genuine love for America. Endorsements If you learned everything you knew about American politics from Matt Lewis, you'd be in pretty good shape. I should know, since I practically do. ?Tucker Carlson, Co-Founder and Editor-in-Chief of"The Daily Caller" Matt Lewis is a conservative provocateur and has an easy way of making complicated issues understandable and leaving the DC jargon off the table. Matt's projects always make you think, reflect and act. ?Governor Rick Perry, 47th Governor of Texas Sarah Palin is one of the most important conservative leaders in America -- and Matt Lewis is one of my favorite writers. Every conservative ought to have a copy of the "Quotable Rogue" in their personal library -- if for no other reason than to refute the distortions and lies constantly hurled at Palin by her detractors. ?Ken Blackwell, former Ohio Secretary of State, senior fellow at the Family Research Council For all the accolades heaped on Mr. Obama for his soaring rhetoric and brilliant, professorial oratory, it's hard to deny that Sarah Palin is one of the most quotable leaders of the 21st century. "The Quotable Rogue" brings that into stunning clarity. And it's not because she's controversial -- it's because she's usually just right. ?S.E. Cupp, political columnist and culture critic, author of "Losing Our Religion: The Liberal Media s Attack on Christianity" As you'll see in Matt's book, the reason for Sarah Palin's popularity is that she speaks in a blunt fashion and exposes truths that the politically correct job-protecting elites are fearful of. She is a far wiser woman than Katie Couric. ?Andrew Breitbart, Breitbart.com, author of "Righteous Indignation" Sarah Palin drives the left crazy and makes many of the Republicans who collaborated with Democrats to destroy our fiscal wellbeing duck and cover. Matt Lewis captures, through Sarah Palin's own words, her wit, wisdom, and basic sense that America remains the last best hope for mankind. ?Erick Erickson, Editor-in-Chief of RedState.com Steadfast in her beliefs and fearless in her demeanor, Sarah Palin has become a leading voice in today s conservative movement and has inspired a new generation of activists. This book of quotes captures the independent spirit of Palin and is a great resource for all of us who are going rogue alongside her. ?Teri Christoph, co-founder, Smart Girl Politics A book like this one is absolutely essential in a world where the media refuses to quote Gov. Palin without liberal doses of snark, vitriol, and hyperbole. It's refreshing to see straight quotes in print so that readers can make up their own minds. ?Adam Brickley, founder, "Draft Sarah Palin for Vice President" "
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WordPress 4.2 is out!
The latest version of WordPress has been released! Version 4.2 has been named "Powell" – you may know of the great jazz pianist Bud Powell. https://videos.files.wordpress.com/e9kH4FzP/wordpress-4-2_dvd.mp4 Communicate and Share The central theme of this release is to help you communicate and share, globally. More Language Support To make communication with other languages, WordPress […]Read more


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If you need both fashion and function in a stroller bag, the Georgi bag from Baby Cargo is meant for you. This one-of-a-kind stroller bag is made to last with brass zippers, solid stitching and an adjustable strap. Its lightweight nylon easily wipes clean. Better still, the Georgi bag is loaded with multipurpose pockets. Plus, you'll bring style to your stroller with its rich colors and accents.
Features: 2 Exterior Side Pockets, Adjustable Shoulder Strap, Zip Closure, Cell Phone Pocket, Multiple Compartments, Removable strap, Stain-resistant changing pad, 5 Exterior Pockets, Stroller strap
Includes: Changing Pad
Material: Polyester
Care and Cleaning: Wipe Clean with a Damp Cloth
Dimensions: 16.5 " H x 16.63 " W x 2.0 " D
Weight: 1.3 Lb.
other Info.
Warranty Information
To obtain a copy of the manufacturer's warranty for this item, please call Target Guest Services at 1-800-591-3869.
- Imported


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Hockey dads raise $1.25M for sports drink company
Aspire Beverage Co., which is marketing a line of low-calorie sports drink for kids, has raised $1.25 million in equity financing, according to a regulatory filing.
The Victoria-based company pitches its drinks as healthy alternatives to sports drinks such as Gatorade. The beverages are 25 calories apiece and feature no artificial ingredients or coloring. The company began selling its drinks at Kowalski's and Lunds and Byerly's stores this spring.
Aspire aims to raise a total of about $4.3 million, according to the regulatory filing.
Bizspace Spotlight
In a May interview, Aspire co-founder John Montague said the company expected to announce a significant investment led by a "major strategic investor" in the coming weeks. Names of investor weren't included in the recent regulatory filing.
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Office of Communications
Explore Resources For Office of Communications
HLS Community Resources
The Office of Communications serves the entire law school community including staff, faculty, alumni and students with a wide array of services and resources.
Media Resources
Members of the news media should contact the HLS Public Information Officer, or consult these web pages for useful information:
Latest News from Harvard Law School
Harvard Law School: The road to marriage equality
Since at least 1983, when Harvard Law student Evan Wolfson '83 wrote a third-year paper exploring a human rights argument for same-sex marriage, Harvard Law School has participated in anticipating, shaping, critiquing, analyzing and guiding the long path toward marriage equality.
Harvard Law Thinks Big: Innovative faculty scholarship in brief
In late May, four Harvard Law faculty members, Charles Fried, Michael Gregory, Kathryn Spier and David Wilkins, each shared a snapshot of innovative research with the HLS community, followed by discussion as part of the 2015 Harvard Law School Thinks Big lecture.
Zittrain delivers chair lecture: 'Love the Processor, Hate the Process'
In a lecture marking his appointment as George Bemis Professor of International Law at Harvard Law School, Jonathan Zittrain '95 addressed the impact of algorithms on our lives—both on and offline—in a lecture titled "Love the Processor, Hate the Process: The Temptations of Clever Algorithms and When to Resist Them."


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Agile Day 2015
- Science & Technology
- 3 Spruce Street, Entrance: One Pace Plaza, New York, NY, 10038
Agile NYC User Group Meeting
Tuesday, September 21, 2010 from 6:00 PM to 9:00 PM (EDT)
Please check http://www.agilenyc.org for updates, location and potential changes of this event.
The user group to discuss topics for agile project managers, software engineers and executives alike. We connect local practitioners and industry experts through monthly in-person face-to-face meetings and through our annual conference day, called "Agile Day". Geographically distanced professionals interested in our activities tune into our podcast: http://feeds.feedburner.com/AgileNYC
Organizers: The (A)gile Team
website: www.agilenyc.org
e-mail: firstname.lastname@example.com
phone: 914 689 6368
Join millions of people on Eventbrite.
In order to purchase these tickets in installments, you'll need an Eventbrite account. Log in or sign up for a free account to continue.


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The fashion industry in Vancouver is growing exponentially and this Meetup group is a fun and efficient way to collaborate with local industry insiders. Join VFI to network with fashion experts, photographers, designers, artists, models, and business owners.
New members must be approved by the Organizer
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I started the group because there wasn't any other type of group like this. I've met some great folks in the group who have become close friends and have also met some amazing business owners.
— Bill, started New York City Gay Craft Beer Lovers
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Raising Arizona
For a while, it seemed as if the only hip-hop action going on in the PHX was being put on by the superfly soldiers of The Blunt Club or our boy Al Page at the Hidden House. Thankfully, the dearth of dopeness has been remedied, thanks in part to the doods behind Raising Arizona, where every Wednesday night, DJs Dirty Napz and Ether (both members of The Feeblez), as well as P Nyce, are spinning hip-hop (both classic and current), funk, jazz, reggae, and soul. The trio's ill allies and other guest DJs and rappers also occasionally get in on the action during each week's edition. The needles start dropping at 10 p.m. and there's no cover. Visit www.myspace.com/raisingaz.
Get the Music Newsletter
Keep your thumb on the local music scene each week with music news, trends, artist interviews and concert listings. We'll also send you special ticket offers and music deals.
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- New Music Night "New Grand Avenue" Aims to Get ThirdSpace Hipping and Hopping
Upcoming Events
Erick MorilloSat., Jul. 4, 11:00am
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- Ravers and Hippies at Electric Forest: A Culture Clash Waiting to Happen?


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Marsanne produces wines with distinctive melon and mineral flavors, rich mouthfeel, and a characteristic nuttiness with age. When blended, its relative restraint and minerality complement more aromatic varietals like Viognier. And in its ancestral home of Hermitage and in other cool climates, Marsanne can make some of the world's most ageable white wines. At Tablas Creek, we use most of our Marsanne in our Côtes de Tablas Blanc.
Marsanne is believed to have originated in the town of Marsanne, near Montélimar in the northern Rhône Valley. The white wines of St-Joseph, Crozes-Hermitage, Hermitage, and St-Péray are made predominantly from Marsanne, often blended with Roussanne. As early as the seventeenth century the white wines from Hermitage were considered among the world's finest. Thomas Jefferson praised white Hermitage as "the first wine in the world without a single exception".
The grape arrived in Australia in the late 1860s, and has been grown successfully in the vineyards of Victoria ever since. Australia has proved an even more hospitable home for the varietal than its native France – 80% of the world's Marsanne is grown in Australia. It arrived in California in the 1980s, and it has becoming an increasingly important component of white Rhône-style blends and is also bottled individually. Qupe Wine Cellars, whose vintner Bob Lindquist has been an early and persistent advocate for the grape, has been instrumental in promoting Marsanne. Qupe began making what has become probably the state's most respected single-varietal Marsanne in 1987. As of 2009, there were 107 acres of Marsanne planted in California, which represents about 3% of the California acreage dedicated to white Rhône varieties.
Plantings of Marsanne in the Northern Rhone have been growing over the last half-century as growers replace the more difficult -- and later ripening -- Roussanne with the easier Marsanne. The vineyards at JL Chave (which have been in the same family since the Middle Ages) were historically equal parts Roussanne and Marsanne, but have been gradually moved to their current composition of 85% Marsanne and just 15% Roussanne.
Marsanne's history is less distinguished in the southern Rhône, and it is not permitted in the wines of Châteauneuf-du-Pape. It is, however, one of the eight white grapes permitted in the Côtes du Rhône appellation. As such, Marsanne is a significant component (usually 30%) of the Coudelet de Beaucastel white blend.
There are approximately three acres of Marsanne planted at Tablas Creek, representing about 7% of our white Rhône production. The climate in Paso Robles is slightly warmer than Marsanne's native northern Rhône Valley, and the varietal here is an aggressive producer with no significant growing problems, though it is sensitive to water levels. Careful monitoring throughout the growing season is often necessary. Marsanne vines produce a relatively heavy crop of loosely clustered berries, and require a secondary fruit pruning (of green or unpollinated clusters) six to eight weeks after the flowering. This practice, coupled with conscientious leaf pulling, encourages uniform ripening. Marsanne ripens right in the middle of the picking season -– later than Viognier, earlier than Roussanne and about at the same time as Grenache Blanc -- and its berries are golden and medium-sized when ripe. It tends to ripen at fairly low sugars; in 2009 its average Brix level was 20.3, the lowest of any variety harvested, and our Marsanne is often around 12% alcohol after fermentation.
We showcase Marsanne's proclivity for displaying the mineral flavors of the soils in which it is grown by fermenting it in stainless steel tanks. In most years, it is blended with a richer, more heady and aromatic Viognier lot sometime 3-4 months after harvest and this resulting tank forms the base of our Côtes de Tablas Blanc from this vintage.
Marsanne is a light straw color, almost green, with moderate acidity and excellent mid-palate richness. Its mineral flavors and aromas, and its low alcohol, make it an ideal blending grape. The varietal has been historically blended with Roussanne, where it tones down the viscosity and acidity of Roussanne and provides a more complex flavor. Although did in early years add Marsanne to our Roussanne-based Esprit de Beaucastel Blanc (most recently in 2001), the varietal is truly given a chance to shine in our Côtes de Tablas Blanc. We have been noting an increase in the intensity and complexity of our Marsanne lots, and hope to make a single-varietal rendition soon.
This article originally appeared in a recent quarterly newsletter. Each newsletter, we spotlight the history and characteristics of one of our Rhône varietals. You can sign up for our mailing list.
You can go back to the summaries of the different Rhône grape varietals.
Since 2007, we have made our En Gobelet exclusively from dry-farmed, head-trained vineyard blocks. The results have been so compelling that we're planning to plant our entire new parcel -- all 55 acres -- in this style over the coming decade. Join us for this vertical tasting of every vintage of En Gobelet, from the 2007 to the newly-blended 2014. We'll also offer, before the tasting, an optional hike led by Viticulturist Levi Glenn through the rugged Scruffy Hill block from which we source most of the wine, and finish with a picnic lunch on our patio. $60 for wine club members and $75 for guests. Reservations are essential; we expect this to sell out. To reserve, email us at firstname.lastname@example.com or call us at 805.237.1231 ext 30. Details & More Events »
We are thrilled to announce that we've received our shipping permit from the great state of Massachusetts. Residents of the Bay State can now order wine or become VINsider Wine Club members. More shipping news »
In August, Antonio Galloni published the results of his annual visit to Tablas Creek, and we were excited to receive such good reviews from this notoriously tough grader. Notes included the 2012 Esprit de Tablas Blanc (92 points; "impeccably refined"), 2011 Panoplie (94 points; "pure elegance"), 2012 Patelin de Tablas (90 points; "a gorgeous wine and a fabulous value"), and the 2012 Esprit de Tablas (93-95 points; "a fascinating Esprit to follow over the coming years"). Read the complete review » More recent press »
June 24, 2015
By: Darren Delmore
I had the distinct pleasure of tagging along last week on a trade visit to the Perrin family's holdings in the Rhone Valley. Our odyssey began with our thirsty quintet of wine professionals packed into an undersized rental car like foie gras terrine as we traversed from Dijon to Valence. I sat shotgun with GPS in hand and snails in my belly as we watched the landscape change from the sunflowers and Charolais beef pastures of Burgundy to the lavender fields and olive groves of the Rhone. Read More »


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Chanel Iman On Chic: 12 Electrifying Looks view
Breakout Style Star: Kiersey Clemons' Top Looks view
Janet Jackson's Top 25 Looks Ever view
If they write Kerry Washington's pregnancy into 'Scandal,' here's how we'd dress her.
Who says Olivia Pope will have to get rid of those chic shift dresses? A...
Who says Olivia Pope will have to get rid of those chic shift dresses? A perfectly ruched style will make her a fab mom-on-the-move.
Maternity drape bodycon dress, Topshop, $58, topshop.com.
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Hostelworld "meet the world" by Lucky Generals
Rating: 5.0
By Jeremy Lee, campaignlive.co.uk, Wednesday, 23 July 2014 05:00PM
Pinnington, who is not being replaced, is planning to dedicate more time to his non-executive directorships. He relinquishes his position with immediate effect but will continue working at Havas in a consultancy role a few days a week for the next 12 months.
Pinnington said: "I have had the most brilliant 30 years at Havas working with talented and, most importantly, fun people. But there is a moment when you feel the time is right to go and do other things that excite you."
He has non-executive jobs at companies including the sustainability agency Futerra and the retailer Heal's.
Pinnington started his career at Havas in 1983, joining WCRS, which was then part of Eurocom/Havas. He became the global chief operating officer at Havas Worldwide in 2007.
This article was first published on campaignlive.co.uk
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Rating: 5.0
Agency: Grey London
Rating: 5.0


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