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EZ Clean – resists stains; is kid and pet friendly.
Available in shapes other than rectangle.
BRAND: Tayse International Trading
COLLECTION: Elegance
COLOR: Red, Ivory, Blue
MADE IN: Turkey
CONSTRUCTION: Machine-Made
MATERIAL: 100% Synthetic (olefin/polypropylene)
THICKNESS: 3⁄8" (approx.)
WARRANTY: 1 year - manufacturer
The Elegance collection offers contemporary, transitional and traditional designs. The variance in color helps to accent the different shapes and increase their appeal. As a whole they form a visual delight to realize your decorating needs.
The digital images we display have the most accurate color possible. However, due to differences in computer monitors, we cannot be responsible for variations...
Rugs Direct recommends you avoid using your vacuum's beater bar on any area rug. For complete information on area rug care and cleaning, please click here.
Rugs Direct in-home delivery is available for selected rugs in this collection.
Standard curb-side delivery of oversized rugs is free on most purchases from Rugs Direct.
However, special "white glove" delivery is available for selected rugs in this collection at additional cost. If you select this option, we will make an appointment to deliver your purchase at a time convenient for you and place the rug in...
This is a great product and great value. Worth every penny.
I did have trouble with this product being damaged at delivery, but the people that dealt with me about the problem could not have been more accommodating to me. I took pictures of the rug as delivered and resolved the problem to my satisfaction via email. I really got a very fair and in my favor deal in the end. Would not hesitate to purchase at Rugs Direct again.


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Gunmetal Black Ep 01
episode 14.24 / 5.00 3,939 Views
Lucina's First Day
A look at the first day of Lucina in Super Smash4.23 / 5.00 12,316 Views
BATMAN v SUPERMAN final showdown
SPOILERS!!4.16 / 5.00 11,297 Views
nothing but awesome
What a nice little animation you have here.
I thought it was awesome. Keep up the good work!
Don't recall doing acid today. Very nice animation by the way. The music fits nicely as well.
i fucking love you. seriously ive been a fan of your animations for ever. your animations have always been fucking awesome and epic frame by frame work. im just so blown away by this one how smooth your life work suddenly got. everything is so smooth and even sexier. i also loved all the character design. the giraffe was the best and made me lol so much
seriously super fucking epic work as always. i fucking love you.
are u cool, u should do animatiopn.
As the deliriously light-hearted music from the beginning suggests, that was just another normal day in the kingdom of imagination. Take it or leave it.

subset: commoncrawl


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For Kara Goucher, balance is everything
After having a baby last year, Kara Goucher is back on the road and ready to rock Boston. The runner-turned-mother talks workouts, discipline and striking a balance.
Updated: April 17, 2011, 12:01 PM ETBy Leslie Goldman
Elite marathoner Kara Goucher, 32, took second at the P.F. Chang's Rock 'n' Roll Arizona Half Marathon in January, just four months after giving birth to her son, Colt. Now she has set her sights on nabbing her best marathon time at the 2012 London Olympics (her current personal record is 2:25:53). With a new book, "Kara Goucher's Running for Women," out and the Boston Marathon going down on Monday, espnW chatted with the Portland, Ore.-based running wunderkind:
espnW: Who introduced ...
Read full story
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As reported by MTV, Abrams and company are still keeping mum about plot or character details. When asked about moments with the mothers of Kirk and Spock, Abrams said, "The what? What flashbacks? I never said flashbacks. Writer/Producer Alex Kurtzman echoed Abrams comments. "We didn't say there were flashbacks."
They were willing to discuss gadgets though. "We intentionally don't talk too much about the story," said Producer Bryan Burk, "but there's all the gadgets you could want. No replicators," (replicators originated in Star Trek: The Next Generation), "but theres warp speed and transporter beams and tricorders and communicators and everything you could want. All the gadgets."
While the futuristic technology is interesting to fans, to the characters in the show, it's just something ordinary and usual. "It's one thing in 'Star Trek' to get all excited and freak out about communicators, but to them, it's like it's the new iPhone," said Abrams. "These are just the tool they're using."
The film is made for the fans who are interested in futuristic technology however, so proper attention is paid to creating the gadgets and getting it right. "We have family members who would disown us if we got any of those wrong," said Writer/Producer Roberto Orci. According to Abrams, "The writers team had 'endless discussions' about how to portray the gadgets, as well as all the other details of 'style' and 'aesthetics.' If you do the bridge of the Enterprise, what does it look like? Does Uhura has the piece in her, or does she not? And if she does, what does it look like? If they have tricorders, what do they look like? Phasers, how do you go from stun to kill, and does anything happen? What does the whole fleet look like? Im telling you, every day, we were figuring this out, how do we take what we know and love and 'Star Trek' and apply it to a modern audience."
Some of the technology will be hidden as "Easter eggs." "There will be appearances of things," said Burk.
To read more, head to the article located here.


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A Rising Star Equestrian Center is an all-breed boarding and training facility. In April of 2004, Ron Beyer and his family purchased A Rising Star Equestrian Center with a commitment to develop it into a first-rate boarding and training facility and with the desire to promote a variety of breeds and riding disciplines.
A Rising Star Equestrian Center has a lesson program for beginner and advanced riders, summer day camps and holds several horse shows each year.
For more information or questions about our facility please contact us at 303.431.4675 or email@example.com
Rising Star's primary owner, Ron has been riding and showing for over 30 years. He started his career in the horse industry concentrating on and competing in team roping, and soon gravitated towards the show ring, showing in English, Under Saddle and Driving in Pleasure, 3 Gaited, 5 Gaited, Park and Fine Harness.
In the fall of 2008, Ron married Robin Cerini. Ron and Robin also live on the property making facility operations and maintenance their first priority. The front gate is closed every night, and nightly checks of the facility and horses are routinely performed.
Robin is also the Horse Show Manager and Registrar on show day, while Ron is the Horse Show Announcer.
|Emily has been riding and showing for 19 years and found her home as the Equestrian Center Manager and Horse Care Coordinator here at A Rising Star. She began her equine journey getting involved in Jefferson County 4-H, learning about all things equine including ownership, riding skills and equitation, safety, feeding programs, overall health and hoof care. She wore many hats within the organization as President, Vice President and Treasurer of her club and President of the Jeffco 4-H Horse Council; she is now a certified 4-H Educational Leader and Riding Instructor. In her career, Emily has been recognized for countless awards within the organization, including back-to-back Year End Overall Grand Champion High Point Awards, Level 3 Horseman in English & Western and the Penny Crosby Leadership Service Award. She also practiced outside skills by participating in the 4-H Horse Judging Team, 4-H Public Speaking Team, judging the 2013 Jefferson County Fair Round Robin Competition and volunteering at The Pegasus Program for Therapeutic Riding. She also spent 10 years of her youth actively achieving the Varsity Team in Westernaires - Mounted Drill Team located in Golden, CO. Emily's primary goal is to provide a friendly and welcoming environment for all owners and students; and for all horses maintain a healthy, safe and caring environment here at A Rising Star.
To schedule your next horseback adventure, or for questions, please call: 303-431-4675
A Rising Star Equestrian Center offers a full range of boarding services featuring routine and reliable maintenance of all facilities and enclosures. This includes weather related attention to heavy snow and run-off in all outside work and enclosure areas as well as entrances to and from.
A Rising Star Equestrian Center offers quality forage products, professional delivery service, and competitive pricing. We deliver hay, straw and supplies throughout the Denver Metro area. Check hay prices and availability here or call us.
We have experienced instructors on-site who teach a variety of disciplines, and if you do not have a horse of your own, we have lesson horses and ponies for riders of all experience levels. Hour-long and half-hour lessons are available for all ages, starting from lead-line lessons for young horse lovers, to show preparation lessons for more advanced riders.


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Graham McPhee spells out some of the cold, hard facts about the dismal state of ME research and politics, and has some suggestions as to what we can do about it ...
Discuss the article on the Forums.
Switching from Famvir to Valcyte.
Discussion in 'Antivirals, Antibiotics and Immune Modulators' started by Ema, Nov 29, 2012.
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Page 2 of 3


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2 bedroom detached bungalow for sale The Lane, Awsworth, NottinghamA well presented detached bungalow situated in a desirable residential location. The property in brief comprises of: Entrance hall, lounge, kitchen/dining room, conservatory, bathroom with four piece white suite and two DOUBLE bedrooms. An internal inspection is highly recommended. More details ›
2 bedroom detached bungalow for sale The Lane, Awsworth, NottinghamMargi Willis Estates are delighted to offer to the market this spacious, modern detached bungalow which is situated in a highly desirable residential location, ideally placed for all local amenities, bus routes, A610 and M1. The accommodation includes: Side Entrance Hall, Lounge, Fitted Kitchen D... More details ›
2 bedroom detached bungalow for sale Park Hill, Nottingham, Nottinghamshire, NG16Charles Newton & Co Estate Agents are pleased to offer for sale this Extended & Fully Upgraded Two Bedroom Bungalow situated on a corner plot .Comprising of; Entrance Hall, Lounge,Dining Room, Kitchen, Utility room, Large Bathroom, Mature Front, Side & Rear Gardens with Views to the Rear & Driveway. More details ›
Added on 06/06/2015 by Charles Newton & Co, Eastwood. Call: 01773 437135 Local call rate
4 bedroom semi-detached house for sale Arches Close, Awsworth**LAST HOUSE REMAINING!** A brand new 4 bedroom three storey family sized semi detached house. situated in this unique private gated development. High Quality finish with all mod cons! floor coverings in, ready to move into! More details ›
Added on 05/06/2015 by Robert Ellis, Stapleford. Call: 0115 798 0180 Local call rate
3 bedroom detached house for sale Tulip Road, AwsworthNO UPWARD CHAIN! This recently refurbished detached family home with fitted breakfast kitchen, lounge, dining room, three bedrooms and a family bathroom. The property benefits from double glazing, gas central heating has front and rear gardens, off road parking and a detached garage. More details ›
£160,000 Offers in Region of
Offers in Region of £160,000
3 bedroom detached bungalow for sale Barlow Drive North, Awsworth, Nottingham, NG16A ROOM WITH A VIEW.... We are delighted to offer to market this well appointed, 2/3 bedroom detached bungalow in the popular village of Awsworth. With easy access to local transport links, including Phoenix Park tram station and Junction 26 of the M1. Briefly comprising entrance hall, modern fitt... More details ›
3 bedroom detached bungalow for sale Barlow Drive North, Awsworth, NottinghamMove & Save offer this Detached Bungalow which has a DETACHED GARAGE and FAR REACHING REAR VIEWS. Located within a CUL DE SAC. Comprises of a hall, lounge diner, fitted kitchen, three bedrooms, shower room, front garden, parking for several vehicles and a well presented LOW MAINTENANCE REAR (con... More details ›
Added on 20/02/2015 by Move & Save, Nottinghamshire. Call: 03331 231242 Local call rate
3 bedroom bungalow for sale Barlow Drive North, Awsworth, Nottingham, NottinghamshireThree bedrooms, one bathroom bungalow and features one reception room. To the front is a block paved drive giving access to the detached garage. To the rear is an enclosed garden with open views. More details ›
3 bedroom detached house for sale Tulip Road, Awsworth, NottinghamMargi Willis Estates are delighted to offer to the market this well presented detached home situated in the village of Awsworth which is conveniently situated for M1 junction 26 and all major link roads. The property is on a generous sized plot with driveway and 21 foot garage. Accommodation... More details ›
3 bedroom detached house for sale Tennyson Square, Awsworth, NottinghamRENSHAW ESTATES ARE THRILLED TO OFFER THIS THREE BEDROOM DETACHED * MODERN REFITTED DINING KITCHEN & BATHROOM * POPULAR CUL-DE-SAC LOCATION * LOUNGE * DRIVEWAY * LOW MAINTENANCE GARDENS * MODERN DECOR THROUGHOUT * FULLY UPVC DG * More details ›
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Final Score: Tampa Bay 3, Toronto 2
Toronto, ON (Sports Network) - Vinny Lecavalier scored twice and Mike Smith was stellar with 37 saves, as the Tampa Bay Lightning defeated the Toronto Maple Leafs, 3-2, at Air Canada Centre.
Jussi Jokinen also scored for Tampa Bay, which has won two of its last three games after the club began the season with a five-game winless skid. Vinny Prospal posted two assists in the victory.
"It's no secret that we dug ourselves a hole and we have to try to get out of it," said Tampa Bay forward Martin St. Louis. "We've got to learn to stay more disciplined. We've got a couple wins and the team chemistry will take care of itself."
Mike Van Ryn and Matt Stajan scored for the Maple Leafs, who saw their two- game win streak halted. Vesa Toskala allowed three goals on 25 shots in the loss.
Smith made two strong saves in succession near the 11-minute mark of the third period to keep the Lightning ahead by one goal. With 10:55 remaining, Smith's glove thwarted a blast from the high slot by Alexei Ponikarovsky and he followed with a stunning save on Niklas Hagman's deflection chance from near the top of the crease.
With 5:10 to go in regulation, the Leafs had a 2-on-1 break but Ponikarovsky's blazing wrist shot was turned aside by Smith, whose best save of the game occurred on a John Mitchell wrister from the outer rim of the left circle with 1:55 on the clock. Smith ended with 13 saves in the final stanza.
The Leafs pulled Toskala for the extra skater in the final minute, but the visitors held on for the one-goal victory.
The Lightning opened the scoring 5:08 into the first period. A soft shot from the right point by Mike Lundin caromed onto the stick of Mark Recchi in the low slot beneath the left circle. Recchi then backhanded a pass across the top of the crease for an easy tap-in by Jokinen past the sliding Toskala.
Less than three minutes later, the Maple Leafs tied it up on a power-play goal by Stajan. Strong forechecking by Ponikarovsky along the rear boards allowed the puck to remain in the Toronto offensive zone and Nik Antropov gained control along the right half-wall. Antropov sent a pass into the low slot, where Stajan redirected the puck and it bounced over the goal line after Smith momentarily had control.
Toronto failed to cash in on a two-man advantage with under 10 minutes remaining in the first period.
"We weren't moving the puck well -- we got away from putting pucks towards the net," said Van Ryn. "We weren't making good passes."
Tampa Bay moved ahead 2-1 with 4:23 to go in the first. Smith started the play when he corralled a dump-in behind the net and sent the disc up the boards along the left side. Prospal's long pass across the neutral zone was intended for Lecavalier, who won a race for the puck, hustled in on a partial breakaway, deked around Toskala and lit the lamp.
At the 4:08 mark of the second stanza, Lecavalier struck again, this time on the power play. Jamal Mayers was whistled for hooking at the 4:03 mark and the visitors needed only five seconds to cash in. Following a faceoff inside the left circle that began the man-advantage, the puck became caught up in sticks and skates in the low slot and it eventually squirted to Lecavalier, who was unchecked and lifted a backhander over Toskala's shoulder from near the right post for a 3-1 lead.
Van Ryn's power-play goal at the 13:15 mark -- assisted by Mikhail Grabovski and Hagman -- of the middle session brought the Leafs within one.
Game Notes
Tampa Bay will play six of its next seven games on the road...The Lightning snapped a six-game skid at Toronto...The Leafs, who converted both of their goals via the power play, had six chances with the extra chances while the visitors were 1-for-5 with the extra skater...Attendance was 19,348.


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You can now invite anyone to meet at your favorite Starbucks.
Limited-time feature
49 year old man | Playa del Rey, CA, USA | Seeking women 39-50 within 20 miles
Active within 24 hours
Hello. Thanks for stopping by. Let's see...I'm...
Hello. Thanks for stopping by. Let's see...I'm originally from the Midwest and have lived in some pretty interesting places to include Japan before moving to LA. I love the weather here, so I can be found biking on the Strand, hiking in various canyons or up at the Getty on a clear day. I enjoy taking road trips up the coast and to Vegas to get out of LA for a while. Photography is a major interest however, I have MANY other interests that are too numerous to list here. My "Match?" Hmmm. I think it all comes down to compatibility. We would end up being the best of friends with similar interests and many differences. We would share the ability to communicate freely and pursue the same basic goals in life together. Thanks for reading and feel free to say "hello" if you like. Take care.
Alumni connections , Coffee and conversation , Cooking , Dining out , Hobbies and crafts , Movies/Videos , Museums and art , Music and concerts , Exploring new areas , Nightclubs/Dancing , Performing arts , Playing sports , Travel/Sightseeing , Watching sports , Wine tasting
Auto racing / Motorcross, Basketball, Billiards / Pool, Cycling, Dancing, Football, Hockey, Other types of exercise, Running, Skiing, Tennis / Racquet sports, VolleyBall, Walking / Hiking, Weights / Machines
I exercise 5 or more times per week.
I like Dogs
Middle of the Road
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What he is looking for
|5'8" (172cms)
|5'0" (152cms) to 5'8" (172cms)
|Body type:
|Athletic and toned
|Slender, About average, Athletic and toned
|No preference
|Auburn / Red, Black, Light brown, Dark brown, Blonde, Dark blonde
|No Way
|No Way
|Social Drinker
|Never, Social Drinker
|Political / Govt / Civil Service / Military
|Administrative / Secretarial, Artistic / Creative / Performance, Executive / Management, Financial / Accounting / Real Estate, Labor / Construction, Legal, Medical / Dental / Veterinary / Fitness, Political / Govt / Civil Service / Military, Retail / Food services, Sales / Marketing, Self-Employed / Entrepreneur, Technical / Science / Computers / Engineering, Travel / Hospitality / Transportation, Other profession, Law enforcement / Security / Military, Fashion / Model / Beauty, Architecture / Interior design
|I'll tell you later
|$25,001 to $35,000, $35,001 to $50,000, $50,001 to $75,000, $75,001 to $100,000, $100,001 to $150,000, $150,001+
|Never Married
|Never Married, Widow / Widower, Divorced
|Have kids:
|Wants kids:
|Not sure
|Someday, Not sure
|Black / African descent
|No preference
|Christian / Protestant
|Christian / Catholic, Christian / Protestant, Spiritual but not religious, Christian / Other
|Bachelors degree
|Associates degree, Bachelors degree, Graduate degree, PhD / Post Doctoral


text:
Should be the ISO news soon. That said, who knows.
Don't know,but I just see tiny manipulations for getting cheaper shares.The lawsuit is a big thing, but we shouldn't forget what we are long waiting for - ISO certification- the game changer. Revenue will come to follow it. Let's keep an eye on it. Must be pretty soon. YIPI
What? R U new for stock market?
and CEO's relatives etc. And so, It is said only 1 - 3 million shares are available. Some said less than 1 million.
Not sure the exact number, but just do your DD. That's what I am saying as a long time holder. Good luck!
Thanks for coming by. YIPI and GSAT together, we'll be winners in the big data and the cloud computing war as we both have that advanced technologies. I say the win win deal.
Past and search the news. Or Global star GSAT (NYSE)
We are still waiting for the ISO news as well. Keep your round eyes on this pappy.
Still imminent. Keep eye on it, I's about coming to be out.
We'll see.
That's being waited.
Next is ISO news. said a big one.
otcmarkets web has it.
FT LAUDERDALE, FL -- (Marketwired) -- 04/09/15 -- ISMS Solutions, Inc. ("ISMS Solutions"), a premier governance, risk management and compliance firm that provides compliance management services and cloud based compliance software solutions that support international regulatory standards and best practices for commercial and government enterprises, announced today that YippyInc. (OTC PINK: YIPI) ("Yippy"), a unified search company, has completed the standard for HIPAA and FISMA compliance under the Information Security Management System (ISMS) implemented by the International Organization for Standardization (ISO).
Yippy is one of many companies which have achieved compliance through ISMS Solutions' Conformance Works™ platform. Conformance Works™ is a proprietary, automated cloud based governance, risk and compliance tool for managing compliance to numerous ISO standards, such as: ISO 27001 (Information Security Management), HIPAA (US Healthcare Security and Privacy Law), FISMA (Federal Information Security Management Act) and many more.
Read IHUB board. lots of news are coming soon!


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2002 Nissan Sentra
I have a 02 Nissan Sentra SER 67000 miles. Every time it rains whether my car is parked or driving the engine stalls. Actually really noticable when I start the car when it has been parked while it has been raining. It does eventually go away after about 5-10 min of driving. I had it serviced about 2 months ago for this problem and they " R & R IGN Coils/Installed silicone.&Quot; I had no problems up until 2 weeks ago. Is there another problem that I should be concerned about?
February 26, 2007.
February 26, 2007.
When i start my truck i hear and ticking noise and it does NOT go away after it warms up or it does not go away at all. When i accelerate the ticking noise gets faster and when im ...
4 answers • 2010 Chevrolet Suburban • 143,000 miles
What would be compatible to use for a replacement engine in my Breeze? I am just looking to get something that would work with it to get it up and running. Can I use a different model ...
1 answer • 1999 Plymouth Breeze • 120,000 miles
If i took out my 91 toyota carolla engine what other cars would it be compatible with?
4 answers • 1991 Toyota Corolla
1990 Toyota Camry mileage: 150,000. 1. The oil warning light is on. 2. There are four quarts of oil in the car. 3. The oil filter is dry. 4. The engine sounds rough. 5. There is noise ...
3 answers • 1990 Toyota Camry • 150,000 miles


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- Remember, fireworks are dangerous
- Wallace asks citizens to fight cuts
- Dispute over state payroll rolls on
- Why fight over free trade confounds partisan divide
- Still no state budget
- Crime control is not the responsibility of landlords
- Fly over to the Poplar Grove Wings and Wheels Museum benefit
- Local leaders warn of budget deadlock's impact
- SHUTDOWN: Illinois preps for the worst
- TRRT Online Edition | July 1-7
Rock Valley College to host groundbreaking ceremony
From press release
The public is invited to a ceremony marking the groundbreaking for the Karl J. Jacobs Center for Science and Math on the main campus of Rock Valley College, 3301 N. Mulford Road, Rockford.
The ceremony will begin at 10 a.m., Thursday, May 20, near the construction site of the new facility. A reception will follow in the AMCORE Bank Commons in the Educational Resource Center.
The naming of the building was announced earlier this year. Dr. Jacobs served as president of Rock Valley College from 1969-1997. During his tenure, multiple academic programs and building projects were formed to serve the region. Highly respected by local leaders, Dr. Jacobs sought to meet community needs through academic and career programs at Rock Valley College.
From the May 19-25, 2010 issue


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Are you David G White?Claim your profile
Publications (70)279.25 Total impact
- SourceAvailable from: Ross C Beier
Article: Characterization of antibiotic and disinfectant susceptibility profiles among Pseudomonas aeruginosa veterinary isolates recovered during 1994-2003[Show abstract] [Hide abstract]
ABSTRACT: Aims: Evaluate susceptibility of Pseudomonas aeruginosa veterinary isolates to antibiotics and disinfectants. Methods and Results: Pseudomonas aeruginosa isolates collected from dogs (n=155) and other animals (n=20) from sixteen states during 1994–2003 were tested for susceptibility. Most isolates were resistant to twenty-one antimicrobials tested, and the highest prevalence of resistance was to β-lactams (93.8%) and sulfonamides (93.5%). Fluoroquinolone resistance did not increase from 1994–2003. Ciprofloxacin and enrofloxacin had a 5% and 16% prevalence of resistance, respectively, while sarafloxacin and nalidixic acid had a prevalence of resistance of 97% and 98%, respectively. Strains were pan-resistant to triclosan, chlorhexidine, highly resistant to benzalkonium chloride, and demonstrated high susceptibility to other disinfectants. Didecyldimethylammonium chloride was the most active ammonium chloride. Inducible resistance was observed to cetyl ammonium halides, chlorhexidine and benzyl ammonium chlorides, which formulate disinfectants used in veterinary clinics and dairies. Organic acid inhibition was associated with the dissociated acid species. Conclusions: Dissociated organic acids appear able to inhibit P. aeruginosa, and rates of fluoroquinolone resistance merit sustained companion animal isolate surveillance. Significance and Impact of the Study: This is the first report of P. aeruginosa susceptibility to 24 disinfectants and illustrates the high resistance of P. aeruginosa to both antibiotics and disinfectants.Journal of Applied Microbiology 12/2014; 118(2):326-342. DOI:10.1111/jam.12707 · 2.39 Impact Factor
Article: Molecular characterization of antibiotic resistant Salmonella Typhimurium and Salmonella Kentucky isolated from pre- and post-chill whole broilers carcasses[Show abstract] [Hide abstract]
ABSTRACT: There is conflicting data regarding whether commercial chilling has any effect on persistence of Salmonella serovars, including antibiotic resistant variants, on chicken carcasses. A total of 309 Salmonella Typhimurium and Salmonella Kentucky isolates recovered from pre- and post-chill whole broiler carcasses were characterized for genetic relatedness using Pulsed Field Gel Electrophoresis (PFGE) and for the presence of virulence factors (invA, pagC, spvC) by PCR and for aerobactin and colicin production by bioassays. A subset of these isolates (n = 218) displaying resistance to either sulfisoxazole and/or ceftiofur [S. Typhimurium (n = 66) and S. Kentucky (n = 152)] were further tested for the presence of associated antibiotic resistance elements (class-I integrons and blaCMY genes) by PCR. All 145 ceftiofur resistant S. Kentucky and S. Typhimurium isolates possessed blaCMY genes. Class-I integrons were only detected in 6.1% (n = 4/66) of sulfisoxazole resistant S. Typhimurium isolates. The PFGE analysis revealed the presence of genetically diverse populations within the recovered isolates but clusters were generally concordant with serotypes and antimicrobial resistance profiles. At a 100% pattern similarity index, thirty-six percent of the undistinguishable S. Typhimurium and 22% of the undistinguishable S. Kentucky isolates were recovered from the same chilling step. All isolates possessed the invA and pagC genes, but only 1.4%possessed spvC. Irrespective of the chilling step, there was a significant difference (P < 0.05) in the production of aerobactin and colicin between S. Typhimurium and S. Kentucky isolates. Taken together, these results indicate that chilling impacted the recovery of particular Salmonella clonal groups but had no effect on the presence of class-I integrons, blaCMY genes, and tested virulence factors.Food Microbiology 04/2014; 38:6-15. DOI:10.1016/j.fm.2013.08.002 · 3.37 Impact Factor
Dataset: JB00297 11 SupplementaryTables
Article: Comparative Genomics of 28 Salmonella enterica Isolates: Evidence for CRISPR-Mediated Adaptive Sublineage Evolution[Show abstract] [Hide abstract]
ABSTRACT: Despite extensive surveillance, food-borne Salmonella enterica infections continue to be a significant burden on public health systems worldwide. As the S. enterica species comprises sublineages that differ greatly in antigenic representation, virulence, and antimicrobial resistance phenotypes, a better understanding of the species' evolution is critical for the prediction and prevention of future outbreaks. The roles that virulence and resistance phenotype acquisition, exchange, and loss play in the evolution of S. enterica sublineages, which to a certain extent are represented by serotypes, remains mostly uncharacterized. Here, we compare 17 newly sequenced and phenotypically characterized nontyphoidal S. enterica strains to 11 previously sequenced S. enterica genomes to carry out the most comprehensive comparative analysis of this species so far. These phenotypic and genotypic data comparisons in the phylogenetic species context suggest that the evolution of known S. enterica sublineages is mediated mostly by two mechanisms, (i) the loss of coding sequences with known metabolic functions, which leads to functional reduction, and (ii) the acquisition of horizontally transferred phage and plasmid DNA, which provides virulence and resistance functions and leads to increasing specialization. Matches between S. enterica clustered regularly interspaced short palindromic repeats (CRISPR), part of a defense mechanism against invading plasmid and phage DNA, and plasmid and prophage regions suggest that CRISPR-mediated immunity could control short-term phenotype changes and mediate long-term sublineage evolution. CRISPR analysis could therefore be critical in assessing the evolutionary potential of S. enterica sublineages and aid in the prediction and prevention of future S. enterica outbreaks.Journal of bacteriology 05/2011; 193(14):3556-68. DOI:10.1128/JB.00297-11 · 2.69 Impact Factor
Article: Horizontal gene transfer of a ColV plasmid has resulted in a dominant avian clonal type of Salmonella enterica serovar Kentucky.[Show abstract] [Hide abstract]
ABSTRACT: Salmonella enterica continues to be a significant cause of foodborne gastrointestinal illness in humans. A wide variety of Salmonella serovars have been isolated from production birds and from retail poultry meat. Recently, though, S. enterica subsp. enterica serovar Kentucky has emerged as one of the prominent Salmonella serovars isolated from broiler chickens. Recent work suggests that its emergence apparently coincides with its acquisition of a ColV virulence plasmid. In the present study, we examined 902 Salmonella isolates belonging to 59 different serovars for the presence of this plasmid. Of the serovars examined, the ColV plasmid was found only among isolates belonging to the serovars Kentucky (72.9%), Typhimurium (15.0%) and Heidelberg (1.7%). We demonstrated that a single PFGE clonal type of S. Kentucky harbors this plasmid, and acquisition of this plasmid by S. Kentucky significantly increased its ability to colonize the chicken cecum and cause extraintestinal disease. Comparison of the completed sequences of three ColV plasmids from S. Kentucky isolated from different geographical locales, timepoints and sources revealed a nearly identical genetic structure with few single nucleotide changes or insertions/deletions. Overall, it appears that the ColV plasmid was recently acquired by a single clonal type S. Kentucky and confers to its host enhanced colonization and fitness capabilities. Thus, the potential for horizontal gene transfer of virulence and fitness factors to Salmonella from other enteric bacteria exists in poultry, representing a potential human health hazard.PLoS ONE 12/2010; 5(12):e15524. DOI:10.1371/journal.pone.0015524 · 3.53 Impact Factor
Article: Evaluation of antimicrobial resistance phenotypes for predicting multidrug-resistant Salmonella recovered from retail meats and humans in the United States.[Show abstract] [Hide abstract]
ABSTRACT: Although multidrug-resistant (MDR) non-Typhi Salmonella (NTS) strains are a concern in food production, determining resistance to multiple antimicrobial agents at slaughter or processing may be impractical. Single antimicrobial resistance results for predicting multidrug resistance are desirable. Sensitivity, specificity, positive predictive value (PPV), and negative predictive value were used to determine each antimicrobial agent's ability to predict MDR phenotypes of human health significance: ACSSuT (resistance to at least ampicillin, chloramphenicol, streptomycin, sulfamethoxazole, tetracycline) in NTS isolates, and MDR-AmpC-SN (resistance to ACSSuT, additional resistance to amoxicillin-clavulanate and to ceftiofur, and decreased susceptibility [MIC >= 2 microg/ml] to ceftriaxone) in NTS serotype Newport. The U.S. National Antimicrobial Resistance Monitoring System determined MICs to 15 or more antimicrobial agents for 9,955 NTS isolates from humans from 1999 to 2004 and 689 NTS isolates from retail meat from 2002 to 2004. A total of 847 (8.5%) human and 26 (3.8%) retail NTS isolates were ACSSuT; 995 (10.0%) human and 16 (2.3%) retail isolates were serotype Newport. Among Salmonella Newport, 204 (20.5%) human and 9 (56.3%) retail isolates were MDR-AmpC-SN. Chloramphenicol resistance provided the highest PPVs for ACSSuT among human (90.5%; 95% confidence interval, 88.4 to 92.3) and retail NTS isolates (96.3%; 95% confidence interval, 81.0 to 99.9). Resistance to ceftiofur and to amoxicillin-clavulanate and decreased susceptibility to ceftriaxone provided the highest PPVs (97.1, 98.1, and 98.6%, respectively) for MDR-AmpC-SN from humans. High PPVs for these agents applied to retail meat MDR-AmpC-SN, but isolate numbers were lower. Variations in MIC results may complicate ceftriaxone's predictive utility. Selecting specific antimicrobial resistance offers practical alternatives for predicting MDR phenotypes. Chloramphenicol resistance works best for ACSSuT-NTS, and resistance to ceftiofur, amoxicillin-clavulanate, or chloramphenicol works best for MDR-AmpC-SN.Journal of food protection 03/2010; 73(3):445-51. · 1.80 Impact Factor
Article: Antimicrobial Resistance, Virulence, and Genotypic Profile Comparison of Campylobacter jejuni and Campylobacter coli Isolated from Humans and Retail Meats[Show abstract] [Hide abstract]
ABSTRACT: A total of 360 spatially and temporally related Campylobacter isolates, including 168 from clinical human cases (Campylobacter jejuni n = 148; Campylobacter coli n = 20) and 192 from retail meats (C. jejuni n = 114; C. coli n = 78), were analyzed for antimicrobial susceptibilities, virulence, and genotypic profiles. Ciprofloxacin-resistant C. jejuni was observed in 13.5% and 19% of the isolates from humans and retail chicken breasts, respectively. Antimicrobial resistance to ciprofloxacin and erythromycin was detected in C. coli isolates recovered from 29% and 16.6% of retail meats and 15% and 5% humans, respectively. Overall, virulence determinants were more prevalent in Campylobacter isolates recovered from retail meats than from humans. C. jejuni isolates from humans were significantly associated with the rakR, dnaJ, and pld genes, whereas C. coli isolates from retail meats were associated with the dnaJ, pld, and virB11 virulence genes. Genotyping of 262 C. jejuni isolates using pulsed-field gel electrophoresis revealed a total of 186 unique SmaI patterns, with 14% of patterns composed of isolates recovered from retail meats and ill humans. All unique groups with indistinguishable SmaI patterns were further analyzed by a second restriction enzyme (KpnI), which revealed limited overlap between isolates from different sources. Significant association between doxycycline-resistant C. jejuni strains recovered from humans and different virulence genes (e.g., cdtB) was identified at the statistical level but not at the genotypic level. In conclusion, significant differences observed in the distribution of antimicrobial resistance profiles, virulence determinants, and genotypic diversity among C. jejuni and C. coli isolates indicate that there are sources other than retail meats that may also contribute to human Campylobacter infections.Foodborne Pathogens and Disease 03/2010; 7(7):835-44. DOI:10.1089/fpd.2009.0487 · 2.09 Impact Factor
Article: A rapid screen of broth enrichments for Salmonella enterica serovars enteritidis, Hadar, Heidelberg, and Typhimurium by Using an allelotyping multiplex PCR that targets O- and H-antigen alleles.[Show abstract] [Hide abstract]
ABSTRACT: Salmonella continues to cause significant foodborne outbreaks, best illustrated with recent outbreaks associated with peanut butter, raw tomatoes, and serrano peppers. To ascertain the likely source of the outbreak, bacterial typing is essential to this process. While PCR has become an important detection tool for pathogens in foods, PCR can also identify strain differences by targeting gene(s) or sequences exhibiting polymorphisms or variability in its distribution within the bacterial population. Over 2,500 Salmonella enterica serovars identified based on antigenic differences in lipopolysaccharide and flagellin have been identified to date. We developed an allelotyping PCR scheme that identifies the O and H alleles associated with S. enterica serovars Enteritidis, Hadar, Heidelberg, Typhimurium, and others, with the same antigen alleles but in different O- and H-allele combinations (e.g., S. enterica Kentucky), and validated it as a screen to identify samples contaminated with these Salmonella serovars. We correctly identified poultry samples containing S. enterica serovars Enteritidis, Kentucky, and Typhimurium from our multiplex screen of primary enrichments of environmental drag swabs. PCR agreed well (kappa values = 0.81 to 1.0) with conventional serotyping methods used to type salmonellae isolated from primary enrichment. Coupled with Salmonella-specific PCR, such as invA, this allelotyping PCR could prove useful in the identification of Salmonella and specific S. enterica serovars present in foods or the environment and could decrease the time and cost associated with conventional serotyping methods.Journal of food protection 10/2009; 72(10):2198-201. · 1.80 Impact Factor
Article: Examination of the Source and Extended Virulence Genotypes of Escherichia coli Contaminating Retail Poultry Meat[Show abstract] [Hide abstract]
ABSTRACT: Extraintestinal pathogenic Escherichia coli (ExPEC) are major players in human urinary tract infections, neonatal bacterial meningitis, and sepsis. Recently, it has been suggested that there might be a zoonotic component to these infections. To determine whether the E. coli contaminating retail poultry are possible extraintestinal pathogens, and to ascertain the source of these contaminants, they were assessed for their genetic similarities to E. coli incriminated in colibacillosis (avian pathogenic E. coli [APEC]), E. coli isolated from multiple locations of apparently healthy birds at slaughter, and human ExPEC. It was anticipated that the retail poultry isolates would most closely resemble avian fecal E. coli since only apparently healthy birds are slaughtered, and fecal contamination of carcasses is the presumed source of meat contamination. Surprisingly, this supposition proved incorrect, as the retail poultry isolates exhibited gene profiles more similar to APEC than to fecal isolates. These isolates contained a number of ExPEC-associated genes, including those associated with ColV virulence plasmids, and many belonged to the B2 phylogenetic group, known to be virulent in human hosts. Additionally, E. coli isolated from the crops and gizzards of apparently healthy birds at slaughter also contained a higher proportion of ExPEC-associated genes than did the avian fecal isolates examined. Such similarities suggest that the widely held beliefs about the sources of poultry contamination may need to be reassessed. Also, the presence of ExPEC-like clones on retail poultry meat means that we cannot yet rule out poultry as a source of ExPEC human disease.Foodborne Pathogens and Disease 07/2009; 6(6):657-67. DOI:10.1089/fpd.2009.0266 · 2.09 Impact Factor
Article: Antimicrobial Resistance-Conferring Plasmids with Similarity to Virulence Plasmids from Avian Pathogenic Escherichia coli Strains in Salmonella enterica Serovar Kentucky Isolates from Poultry[Show abstract] [Hide abstract]
ABSTRACT: Salmonella enterica, a leading cause of food-borne gastroenteritis worldwide, may be found in any raw food of animal, vegetable, or fruit origin. Salmonella serovars differ in distribution, virulence, and host specificity. Salmonella enterica serovar Kentucky, though often found in the food supply, is less commonly isolated from ill humans. The multidrug-resistant isolate S. Kentucky CVM29188, isolated from a chicken breast sample in 2003, contains three plasmids (146,811 bp, 101,461 bp, and 46,121 bp), two of which carry resistance determinants (pCVM29188_146 [strAB and tetRA] and pCVM29188_101 [bla(CMY-2) and sugE]). Both resistance plasmids were transferable by conjugation, alone or in combination, to S. Kentucky, Salmonella enterica serovar Newport, and Escherichia coli recipients. pCVM29188_146 shares a highly conserved plasmid backbone of 106 kb (>90% nucleotide identity) with two virulence plasmids from avian pathogenic Escherichia coli strains (pAPEC-O1-ColBM and pAPEC-O2-ColV). Shared avian pathogenic E. coli (APEC) virulence factors include iutA iucABCD, sitABCD, etsABC, iss, and iroBCDEN. PCR analyses of recent (1997 to 2005) S. Kentucky isolates from food animal, retail meat, and human sources revealed that 172 (60%) contained similar APEC-like plasmid backbones. Notably, though rare in human- and cattle-derived isolates, this plasmid backbone was found at a high frequency (50 to 100%) among S. Kentucky isolates from chickens within the same time span. Ninety-four percent of the APEC-positive isolates showed resistance to tetracycline and streptomycin. Together, our findings of a resistance-conferring APEC virulence plasmid in a poultry-derived S. Kentucky isolate and of similar resistance/virulence plasmids in most recent S. Kentucky isolates from chickens and, to lesser degree, from humans and cattle highlight the need for additional research in order to examine the prevalence and spread of combined virulence and resistance plasmids in bacteria in agricultural, environmental, and clinical settings.Applied and Environmental Microbiology 07/2009; 75(18):5963-71. DOI:10.1128/AEM.00786-09 · 3.95 Impact Factor
Article: Molecular Analysis of Escherichia coli from Retail Meats (2002-2004) from the United States National Antimicrobial Resistance Monitoring System[Show abstract] [Hide abstract]
ABSTRACT: The origins and virulence potential of meat product-associated Escherichia coli are undefined. Two hundred eighty-seven E. coli isolates (145 resistant and 142 susceptible to trimethoprim-sulfamethoxazole, nalidixic acid, and/or ceftiofur), recovered by the United States National Antimicrobial Monitoring System from retail beef, pork, chicken, and turkey products (from Oregon, Tennessee, Georgia, and Maryland, 2002-2004) underwent polymerase chain reaction testing for phylogenetic groupings and 59 virulence-associated genes. However analyzed, resistant and susceptible isolates differed minimally according to the assessed characteristics. In contrast, the 4 meat types differed greatly for multiple individual traits and aggregate virulence scores. Poultry isolates exhibited virulence genes associated with avian pathogenic E. coli; beef isolates exhibited traits associated with E. coli from diseased cattle. Overall, 20% of isolates qualified as extraintestinal pathogenic E. coli, with poultry isolates exhibiting significantly higher virulence scores than beef and pork isolates (P < .001). Within this systematically collected, geographically distributed sample of recent retail meat isolates, the carriage of extraintestinal pathogenic E. coli virulence genes in antimicrobial-resistant and antimicrobial-susceptible E. coli appeared similar, whereas isolates from different types of meat differed, consistent with on-farm acquisition of resistance within host species-specific E. coli populations. A substantial minority of meat-source E. coli (whether susceptible or resistant) may represent potential human pathogens.Clinical Infectious Diseases 07/2009; 49(2):195-201. DOI:10.1086/599830 · 9.42 Impact Factor
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ABSTRACT: Multidrug resistance (MDR) plasmids belonging to the IncA/C plasmid family are widely distributed among Salmonella and other enterobacterial isolates from agricultural sources and have, at least once, also been identified in a drug-resistant Yersinia pestis isolate (IP275) from Madagascar. Here, we present the complete plasmid sequences of the IncA/C reference plasmid pRA1 (143,963 bp), isolated in 1971 from the fish pathogen Aeromonas hydrophila, and of the cryptic IncA/C plasmid pRAx (49,763 bp), isolated from Escherichia coli transconjugant D7-3, which was obtained through pRA1 transfer in 1980. Using comparative sequence analysis of pRA1 and pRAx with recent members of the IncA/C plasmid family, we show that both plasmids provide novel insights into the evolution of the IncA/C MDR plasmid family and the minimal machinery necessary for stable IncA/C plasmid maintenance. Our results indicate that recent members of the IncA/C plasmid family evolved from a common ancestor, similar in composition to pRA1, through stepwise integration of horizontally acquired resistance gene arrays into a conserved plasmid backbone. Phylogenetic comparisons predict type IV secretion-like conjugative transfer operons encoded on the shared plasmid backbones to be closely related to a group of integrating conjugative elements, which use conjugative transfer for horizontal propagation but stably integrate into the host chromosome during vegetative growth. A hipAB toxin-antitoxin gene cluster found on pRA1, which in Escherichia coli is involved in the formation of persister cell subpopulations, suggests persistence as an early broad-spectrum antimicrobial resistance mechanism in the evolution of IncA/C resistance plasmids.Journal of bacteriology 06/2009; 191(15):4750-7. DOI:10.1128/JB.00189-09 · 2.69 Impact Factor
Article: Characterization of antimicrobial resistance in Salmonella enterica serotype Heidelberg isolated from food animals.[Show abstract] [Hide abstract]
ABSTRACT: Fifty-eight Salmonella enterica serovar Heidelberg isolates isolated from food animals were tested for antimicrobial susceptibilities and further characterized for select antimicrobial resistance genes, plasmid carriage, class 1 integrons, and genetic relatedness using pulsed-field gel electrophoresis (PFGE). Seventy-two percent of isolates displayed resistance to at least one of the antimicrobial agents tested, while 24% exhibited resistance to eight or more antimicrobial agents. Resistance was most commonly observed to tetracycline (71%), streptomycin (62%), and kanamycin (52%). Isolates obtained from cattle and swine displayed the highest rates of resistance while isolates from chickens more often displayed susceptibility to the tested antimicrobials. When resistance was detected, a corresponding resistance gene was detected in 97.3% of the isolates. Thirteen percent of the isolates contained class 1 integrons containing at least one resistance gene, most often either the aadA or dhfrA genes, which are often associated with resistance to streptomycin and trimethoprim, respectively. Twenty isolates contained plasmids estimated to be at least 75 kb in size, 17 of which exhibited resistance to five or more antimicrobial agents. Thirty PFGE patterns were generated among the 58 isolates tested using XbaI, indicating extensive heterogeneity among this serotype across different animal origins. Results confirm the presence of multidrug-resistance (MDR) phenotypes among food animal isolates of serovar Heidelberg, especially those obtained from mammalian species. The observed MDR was typically associated with the presence of large plasmids.Foodborne Pathogens and Disease 03/2009; 6(2):207-15. DOI:10.1089/fpd.2008.0172 · 2.09 Impact Factor
Article: IncA/C Plasmid-Mediated Florfenicol Resistance in the Catfish Pathogen Edwardsiella ictaluri[Show abstract] [Hide abstract]
ABSTRACT: Florfenicol (FFC) has recently been approved by the Food and Drug Administration for the treatment of several bacterial diseases of cultured fish species in the United States (US), including the treatment of enteric septicemia of catfish (ESC) caused by Edwardsiella ictaluri. ...Antimicrobial Agents and Chemotherapy 12/2008; 53(2):845-6. DOI:10.1128/AAC.01312-08 · 4.45 Impact Factor
Article: Rapid screening of Salmonella enterica serovars Enteritidis, Hadar, Heidelberg and Typhimurium using a serologically-correlative allelotyping PCR targeting the O and H antigen alleles[Show abstract] [Hide abstract]
ABSTRACT: Classical Salmonella serotyping is an expensive and time consuming process that requires implementing a battery of O and H antisera to detect 2,541 different Salmonella enterica serovars. For these reasons, we developed a rapid multiplex polymerase chain reaction (PCR)-based typing scheme to screen for the prevalent S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium. By analyzing the nucleotide sequences of the genes for O-antigen biosynthesis including wba operon and the central variable regions of the H1 and H2 flagellin genes in Salmonella, designated PCR primers for four multiplex PCR reactions were used to detect and differentiate Salmonella serogroups A/D1, B, C1, C2, or E1; H1 antigen types i, g, m, r or z10; and H2 antigen complexes, I: 1,2; 1,5; 1,6; 1,7 or II: e,n,x; e,n,z15. Through the detection of these antigen gene allele combinations, we were able to distinguish among S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium. The assays were useful in identifying Salmonella with O and H antigen gene alleles representing 43 distinct serovars. While the H2 multiplex could discriminate between unrelated H2 antigens, the PCR could not discern differences within the antigen complexes, 1,2; 1,5; 1,6; 1,7 or e,n,x; e,n,z15, requiring a final confirmatory PCR test in the final serovar reporting of S. enterica. Multiplex PCR assays for detecting specific O and H antigen gene alleles can be a rapid and cost-effective alternative approach to classical serotyping for presumptive identification of S. enterica serovars Enteritidis, Hadar, Heidelberg, and Typhimurium.BMC Microbiology 11/2008; 8(1):178. DOI:10.1186/1471-2180-8-178 · 2.98 Impact Factor
- Preharvest and Postharvest Food Safety: Contemporary Issues and Future Directions, 02/2008: pages 239 - 256; , ISBN: 9780470752579
- Microbial Food Safety in Animal Agriculture: Current Topics, 02/2008: pages 35 - 44; , ISBN: 9780470752616
Article: Antimicrobial susceptibility and distribution of antimicrobial-resistance genes among Enterococcus and coagulase-negative Staphylococcus isolates recovered from poultry litter.[Show abstract] [Hide abstract]
ABSTRACT: Data on the prevalence of antimicrobial resistant enterococci and staphylococci from the poultry production environment are sparse in the United States. This information is needed for science-based risk assessments of antimicrobial use in animal husbandry and potential public-health consequences. In this study, we assessed the susceptibility of staphylococci and enterococci isolated from poultry litter, recovered from 24 farms across Georgia, to several antimicrobials of veterinary and human health importance. Among the 90 Enterococcus isolates recovered, E. hirae (46%) was the most frequently encountered species, followed by E. faecium (27%), E. gallinarum (12%), and E. faecalis (10%). Antimicrobial resistance was most often observed to tetracycline (96%), followed by clindamycin (90%), quinupristin-dalfopristin (62%), penicillin (53%), erythromycin (50%), nitrofurantoin (49%), and clarithromycin (48%). Among the 110 staphylococci isolates recovered, only coagulase-negative staphylococci (CNS) were identified with the predominant Staphylococcus species being S. sciuri (38%), S. lentus (21%), S. xylosus (14%) and S. simulans (12%). Resistance was less-frequently observed among the Staphylococcus isolates for the majority of antimicrobials tested, as compared with Enterococcus isolates, and was primarily limited to clarithromycin (71%), erythromycin (71%), clindamycin (48%), and tetracycline (38%). Multidrug resistance (MDR) phenotypes were prevalent in both Enterococcus and Staphylococcus; however, Enterococcus exhibited a statistically significant difference in the median number of antimicrobials to which resistance was observed (median = 5.0) compared with Staphylococcus species (median = 3.0). Because resistance to several of these antimicrobials in gram-positive bacteria may be attributed to the shuttling of common drug-resistance genes, we also determined which common antimicrobial-resistance genes were present in both enterococci and staphylococci. The antimicrobial resistance genes vat(D) and erm(B) were present in enterococci, vgaB in staphylococci, and mobile genetic elements Tn916 and pheromone-inducible plasmids were only identified in enterococci. These data suggest that the disparity in antimicrobial-resistance phenotypes and genotypes between enterococci and staphylococci isolated from the same environment is, in part, because of barriers preventing exchange of mobile DNA elements.Avian Diseases 01/2008; 51(4):884-92. DOI:10.1637/7973-032607-REGR.1 · 1.11 Impact Factor
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ABSTRACT: This study was conducted to determine the prevalence and antimicrobial resistance of Salmonella isolates recovered from processed poultry. Four hundred eighty pre- and postchill whole broiler chicken carcasses were collected from a poultry processing plant between July 2004 and June 2005. Water samples also were collected at the entrance and exit of the chiller. After preenrichment, carcass and water samples were analyzed for the presence of Salmonella using the automated BAX system followed by traditional culture methods. The proportions of pre- and postchill carcasses that were positive for Salmonella were 88.4 and 84.1%, respectively. Ninety-two percent of water samples collected at the entrance of the chiller were positive for Salmonella, but all exit samples were negative. There was no significant difference in the prevalence of Salmonella between pre- and postchill carcasses (P > 0.05). Salmonella isolates recovered were serotyped and tested for susceptibility to antimicrobials. Thirteen serotypes were identified; the most common were Salmonella Kentucky (59.5%) and Salmonella Typhimurium (17.8%). Three hundred thirty-nine (79.8%) of the isolates were resistant to at least one antimicrobial, and 53.4% were resistant to three or more antimicrobials. Resistance was most often observed to tetracycline (73.4% of isolates), ampicillin (52.9%), amoxicillin-clavulanic acid (52%), ceftiofur (51.7%), streptomycin (35.2%), and sulfisoxazole (21.8%). These results indicate the high prevalence of Salmonella contamination in whole broiler carcasses, and a large number of these Salmonella isolates were resistant to commonly used antimicrobials.Journal of food protection 12/2007; 70(11):2466-72. · 1.80 Impact Factor
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ABSTRACT: The use of antimicrobial agents in food animals can select for resistant bacterial pathogens that may be transmitted to humans via the commercial meat supply. In the USA, the FDA's Center for Veterinary Medicine regulatory duties require a determination that antimicrobial drugs are safe and effective for use in food animals. In addition, a qualitative assessment of risks to human health from antimicrobial resistance requires development. This risk assessment process is supported by data generated by the FDA's National Antimicrobial Resistance Monitoring System (NARMS) for enteric bacteria. NARMS data on antimicrobial susceptibility among Salmonella, Campylobacter, Escherichia coli and Enterococcus is collected. Research activities defining the genetic bases of resistance helps to understand the potential public health risks posed by the spread of antimicrobial resistance from food animal antimicrobial use. These activities help insure that antimicrobials are used judiciously to promote human and animal health.Future Microbiology 11/2007; 2(5):493-500. DOI:10.2217/174609188.8.131.523 · 3.82 Impact Factor
Top Journals
U.S. Food and Drug Administration
Washington, Washington, D.C., United States
- • Office of Research
- • Center for Veterinary Medicine
Johns Hopkins University
Baltimore, MD, United States
- Department of Biology
University of Maryland, Baltimore
Baltimore, MD, United States
- Institute for Genome Sciences
Biomedical Research Institute, RockvilleMaryland, United States
Iowa State University
Ames, Iowa, United States
- Department of Veterinary Microbiology and Preventive Medicine
North Dakota State University
Fargo, North Dakota, United States
- Department of Veterinary and Microbiological Sciences
United States Department of Agriculture
Washington, Washington, D.C., United States
- Agricultural Research Service (ARS)
University of Maryland, College Park
College Park, MD, United States
- • Department of Nutrition and Food Science
- • Department of Cell Biology & Molecular Genetics
University of Georgia
Athens, GA, United States
- • Department of Infectious Diseases
- • College of Veterinary Medicine
University of Central Arkansas
Conway, AR, United States
- Department of Biology
China Agricultural UniversityPeping, Beijing, China
Pennsylvania State UniversityUniversity Park, Maryland, United States


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The Magic of Reality
How We Know What's Really True
By Richard Dawkins; Dave McKean (Illustrator)
(Free Press, Hardcover, 9781439192818, 272pp.)
Publication Date: October 4, 2011
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Magic takes many forms. Supernatural magic is what our ancestors used in order to explain the world before they developed the scientific method. The ancient Egyptians explained the night by suggesting the goddess Nut swallowed the sun. The Vikings believed a rainbow was the gods' bridge to earth. The Japanese used to explain earthquakes by conjuring a gigantic catfish that carried the world on its back—earthquakes occurred each time it flipped its tail. These are magical, extraordinary tales. But there is another kind of magic, and it lies in the exhilaration of discovering the real answers to these questions. It is the magic of reality—science.
Packed with clever thought experiments, dazzling illustrations and jaw-dropping facts, The Magic of Reality explains a stunningly wide range of natural phenomena. What is stuff made of? How old is the universe? Why do the continents look like disconnected pieces of a puzzle? What causes tsunamis? Why are there so many kinds of plants and animals? Who was the first man, or woman? This is a page-turning, graphic detective story that not only mines all the sciences for its clues but primes the reader to think like a scientist as well.
Richard Dawkins, the world's most famous evolutionary biologist and one of science education's most passionate advocates, has spent his career elucidating the wonders of science for adult readers. But now, in a dramatic departure, he has teamed up with acclaimed artist Dave McKean and used his unrivaled explanatory powers to share the magic of science with readers of all ages. This is a treasure trove for anyone who has ever wondered how the world works. Dawkins and McKean have created an illustrated guide to the secrets of our world—and the universe beyond—that will entertain and inform for years to come.
Richard Dawkins is a Fellow of the Royal Society and was the inaugural holder of the Charles Simonyi Chair of Public Understanding of Science at Oxford University. He is the acclaimed author of many books including The Selfish Gene, Climbing Mount Improbable, Unweaving the Rainbow, The Ancestor's Tale, The God Delusion, and The Greatest Show on Earth. Visit him at RichardDawkins.net.


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06A Journey Into Darkness: We Went Mountain Biking in the Abandoned Mines of Slovenia
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Who are the new Dylans, Public Enemys, Simones and De La Rochas of 2014? If you're a fan of Sly and the Family Stone or Marvin Gaye, you'll want to listen to D'Angelo's new album, Black Messiah. Continue reading
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- December 18, 2014
- December 18, 2014
The tall tales of the phone and cable lobby keep crumbling down. Continue reading
- December 17, 2014Big victory for anti-fracking activists in New York State today as Governor Cuomo announces a permanent ban on fracking in the state.
- December 16, 2014 | Perspectives
See what we've been reading. Continue reading
- December 16, 2014
The test of whether a person can afford a lawyer is often so extreme that even the most indigent of defendants may be deemed unworthy of counsel.
Continue reading
- December 12, 2014
Protests against racist injustice — triggered by the failure of the criminal justice system to indict the killers of Michael Brown and Eric Garner — has propelled Obama to recall his community organizing roots. Continue reading
- December 10, 2014 | ActivismAt Wednesday's ceremony in Oslo, the heroic Pakistani asks, "Why is it that giving guns is so easy but giving books is so hard?"
- December 10, 2014
"While not every traditional variety tastes great or looks great, its genetics may be invaluable 50 or 100 years from now when the climate is different." Continue reading
- December 9, 2014 | Inequality
Bill asked people to write in with their own stories after our report on how wealthy investors are driving up real estate values in New York City and pushing out working people of ordinary means. Continue reading
- December 9, 2014
Universities light the way forward for the next generation — they shouldn't be beholden to an industry that relies on the pollution of the past. Continue reading


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Calling all Amazon Prime members! Amazon has started a fantastically awesome new promotion - save $10 off any $30 order of groceries. Just use the coupon code 10OFFGRO at checkout. There are lots of ways to stack this savings with even more … Read More →
*HOT* $10 off $30 Grocery Order at Amazon (Prime Members Only)
Doc McStuffins Diagnose-a-Tosis Bone Doctor Set – $9.97 **50% Off**
Got a Doc McStuffins fan at home? (We love her!) Grab the Just Play Doc McStuffins Diagnose-a-Tosis Bone Doctor Set for only $9.97, down from $19.99. Two-day shipping is free with your Amazon Prime account. If you're not a member, you can get … Read More →
Expo Assorted Colors Fine Point Dry Erase Markers, 12-Pack – $6.75 (Down from $15.99)
These are back in stock! Get a jump-start on your school supply shopping for the fall! Amazon has a fantastic price on these Expo 2 Low-Odor Dry Erase Markers, Fine Point, 12-Pack, Assorted Colors. Get them now for just $6.75, originally … Read More →
Lalaloopsy Moments in Time Jewel Mini Doll — Only $2.98 (As Add-On)
Gift closet alert! Get this cute Lalaloopsy Moments in Time Jewel Mini Doll for $2.98 right now (reg. $8.99). This is the lowest price on record by far!
- Every character is designed from their special … Read More →
Cutter All Family 15-Count Insect Repellent Mosquito Wipes — Just $2.90 (As Add-On)
Right now at Amazon, you can pick up this 15-count box of Cutter All Family Insect Repellent Mosquito Wipes for just $2.90 right now, regularly $6.99. This is the very lowest they've gone for on Amazon. I think these would be perfect for keeping … Read More →
One-Day iOtti Sale at Amazon | Car Mounts and Chargers Up to 40% Off
Could you use a car mount to hold you're phone while driving, or a car charger? There is a one-day sale on iOttie Car Mounts and Chargers going on today at Amazon, with nine options to choose from up to 40% off. Here are a few that are … Read More →
Kirkland Signature 13-Gallon Drawstring Kitchen Trash Bags, 200 Count — Only $13.49 Shipped!
Update: If the price doesn't show up for you, check over on the right under "Other Sellers on Amazon". That's where I'm seeing it now! Running low on kitchen trash bags? Amazon has this Kirkland Signature 13-Gallon Drawstring Kitchen Trash … Read More →
Nite Ize S-Biner Household Clips (3-Pack) — Only $3.94 (As Add-On)
Have you used these S-biner clips before? They're great for clipping items together securely such as zippers on luggage, hanging planters, and more! Well, right now Amazon has this 3-pack of Nite Ize S-Biner Household Clips marked down to the … Read More →
Recyclebank | 75 New Points
There are some new opportunities to add some quick and easy points to your Recyclebank account. First, click on the banner above to learn about how our energy needs affect the environment. This one is worth 25 points. Then, earn another … Read More →
Ikea Microwave Lid — Just $1.29 (As Add-On)
Amazon has this highly-rated Ikea Microwave Lid in Blue marked down to just $1.29, regularly $10 - lowest price! We use this in our microwave in lieu of Saran Wrap. It also fits perfectly over a dinner plate. Our is Rubbermaid, which I bought at … Read More →


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User account
Decision 2016
Latest Comments
- sentairanger • 57 sec ago
Do you even know what Marxism even is? I bet not.
- RepubAnon • 1 min ago
Not quite - theft is always against the law. It's just that the folks who write the laws make sure that rich people taking away pensions from poor people is legal:
Financier Ronald Perelmen took over Revlon in 1985, shut down its pension plan and got control of more than $100 million in surplus pension assets. Charles Hurwitz took over Pacific Lumber, closed down its pension and used $55 million in surplus pension assets to help finance his buyout. After many such cases, Congress slapped a 50% excise tax on "reversions" in 1990, and pension terminations at large companies slowed almost to a halt.
...But killing the plans for their money is again looking like a tempting option in certain situations, such as when companies have heavy debts or are acquisition candidates -- in which case the pension surplus can be a kind of dowry to suitors.
Fueling the trend is a little-known tax angle. When a corporation terminates a pension plan, it can avoid the onerous 50% excise tax and pay only 20%. All it has to do is put one-quarter of the plan's surplus into a "replacement plan."
Source: Pension Terminations Become Temptations for Some Employers By Ellen E. Schultz, Staff Reporter of THE WALL STREET JOURNAL June 15, 1999
- sentairanger • 2 min ago
The US should replace its boring stars and stripes flag with the stars and rainbow stripes flag that is flown during pride month. Hell the battle flag can be repurposed as a flag of equality by giving it the rainbow makeover.
- James Ratliff • 3 min ago
Look i'm 64 years old. Been married to the same woman for over 36 years now. I have a daughter and two grandchildren. I just don't understand how allowing gay people the same rights that I have hurts me?
Can one of these RWNJ's explain in plain simple English how this hurts their own marriage or anyone else's?
- 1Smith1 • 4 min ago
Wrong again, troll.


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The second day of free agency was mostly quiet for the 49ers, who entertained massive free-agent guard Leonard Davis on Wednesday while Adam Snyder signed a five-year deal with the Arizona Cardinal
From Comcast SportsNetTAMPA, Fla. (AP) -- Greg Schiano glanced to his left where the three newest members of the Tampa Bay Buccaneers were seated and smiled broadly.
On the eve of free agency, the 49ers signed one of the all-time great producers at the position of their biggest offseason need.Receiver Randy Moss was paced through a workout on the 49ers' Sa
So heres the question you, the 49er fan, have to ask yourself:
Is Jim Harbaugh powerless to resist the bad angel on Randy Moss shoulder?
The 49ers are beginning their search to strength the wide receiver position on Monday.Coach Jim Harbaugh will pace veteran Randy Moss through a workout.


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Where Are They Now
'Singles': Where Are They Now?
Eddie, Jeff and Stone are still members of Pearl Jam, which has sold approximately 60 million albums worldwide. Their most recent record was 2009's "Backspacer." In 2011, they released "Pearl Jam Twenty," a documentary about the band's first two decades together.
Eddie is currently married to model Jill McCormick; the couple has two daughters, Olivia and Harper. Always politically active, Vedder was recently active in the movement to free the West Memphis Three. Jeff, whose longtime girlfriend is Pandora Andre-Beatty, released a solo album in 2008. The following year, he was mugged outside of an Atlanta recording studio. Stone (not pictured) is married to Liz Weber, with whom he has a daughter, Vivian Sparks. He released a solo album in 2001.
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text: due to Brittany Ferries cancelling our crossing (quite a common occurrence) we found ourselves outside Cherbourg with nowhere to stay except to sleep in the car. At 2200hrs we thought a hotel unlikely but drove past this hotel on our way to the port. For 67euros we got a twin room. desk staff were so nice. we didn't expect much... More


text: Study Search Results Your query returned 5 results. Results per page: 25 50 100 Sort by: Title A-Z Released/Updated Time Period Most Cited in ICPSR Bib. (?) Study Title/Investigator 1. Chicago Women's Health Risk Study, 1995-1998 (ICPSR 3002) Block, Carolyn Rebecca 2. Experiences and Needs of Formerly Intimate Stalking Victims in Southeastern Pennsylvania, 1991-1995 (ICPSR 2899) Brewster, Mary P. 3. Homicides in Chicago, 1965-1995 (ICPSR 6399) Block, Carolyn Rebecca; Block, Richard L.; Illinois Criminal Justice Information Authority 4. Violence Against Women: Developmental Antecedents Among Black, Caucasian, and Hispanic Women in the United States, 1987-1988 and 1992-1994 (ICPSR 3293) Jasinski, Jana L. 5. Women and Violence in Chicago, Illinois, 1994-1995 (ICPSR 2958) Lloyd, Susan [1]


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U.S. Insular Areas:
Multiple Factors Affect Federal Health Care Funding
GAO-06-75: Published: Oct 14, 2005. Publicly Released: Oct 17, 2005.
Additional Materials:
- Highlights Page:
- Full Report:
- Accessible Text:
(202) 512-7059
Office of Public Affairs
(202) 512-4800
Five insular areas of the United States--American Samoa, the Commonwealth of the Northern Mariana Islands (CNMI), Guam, Puerto Rico, and the U.S. Virgin Islands--benefit from federal health care financing and grant programs that help fund health care services to their over 4 million residents. However, notable differences exist in how the programs are funded or operate in the insular areas, such as statutory limits on federal Medicaid funding to the insular areas that do not apply in the states. To help understand these differences, GAO was asked to identify (1) the key sources of federal health care funding in the insular areas, (2) differences between insular areas and the states in the methods used to allocate these funds, and (3) differences in spending levels per individual between insular areas and the states. In commenting on a draft of this report, American Samoa, CNMI, and Puerto Rico suggested the need for additional information on certain issues, such as implications of statutory limits on federal Medicaid spending and a more comprehensive analysis of local circumstances that affect the availability and costs of health care services.
Multiple federal programs fund health care services in the insular areas. Federal health care financing programs--Medicare, Medicaid, and the State Children's Health Insurance Program (SCHIP)--represented nearly 90 percent of the $2.2 billion in health care funding to these areas in fiscal year 2003, with Medicare alone representing over three-quarters of total funding. The Departments of Health and Human Services (HHS) and the Interior (DOI) also provide grants to the insular areas. Significant variation exists among the insular areas in terms of the distribution of funds by these sources, largely due to the number of Medicare beneficiaries in each area. The methods used to allocate these federal funds to insular areas often differ from methods used in the states. For example, Medicare pays hospitals in most insular areas based on their costs rather than the prospective payment system used for most hospitals in the states. Similarly, federal funding for Medicaid and SCHIP is subject to statutory limits that do not apply to states, including minimum federal contributions and a cap on federal Medicaid payments. In addition, certain HHS grants use different rules to determine insular areas' funding. Differences in allocation methods as well as other factors contribute to lower spending levels per individual in the insular areas compared to the states. For example, Medicare spending per beneficiary in the insular areas was less than half the amount it was in the states, due in part to differences in payment policies and to beneficiaries' lower utilization of services. In addition, the statutory limits on federal Medicaid funding in these areas contributed to lower federal Medicaid per capita payments in the five insular areas compared to the national average. However, in light of limits on federal funding, the insular areas are not held accountable for covering all Medicaid benefit requirements, such as nursing facility services that represent nearly one-third of Medicaid expenditures in the states. Insular areas benefit from certain HHS grant allocation formulas that result in higher per capita payments to them than the states, on average.
Jun 29, 2015
Medicaid:Service Utilization Patterns for Beneficiaries in Managed CareGAO-15-481: Published: May 29, 2015. Publicly Released: Jun 29, 2015.
Jun 24, 2015
Medicaid Demonstrations:More Transparency and Accountability for Approved Spending Are NeededGAO-15-715T: Published: Jun 24, 2015. Publicly Released: Jun 24, 2015.
Jun 22, 2015
Defense Health Care:DOD Needs to Clarify Policies Related to Occupational and Environmental Health Surveillance and Monitor Risk Mitigation ActivitiesGAO-15-487: Published: May 22, 2015. Publicly Released: Jun 22, 2015.
Jun 11, 2015
Mental Health:Better Documentation Needed to Oversee Substance Abuse and Mental Health Services Administration GranteesGAO-15-405: Published: May 12, 2015. Publicly Released: Jun 11, 2015.
Jun 10, 2015
VA Health Care:Improvements Needed to the Monitoring of Antidepressant Use for Major Depressive Disorder and the Accuracy of Suicide DataGAO-15-648T: Published: Jun 10, 2015. Publicly Released: Jun 10, 2015.
Jun 2, 2015
Medicaid:CMS Could Take Additional Actions to Help Improve Provider and Beneficiary Fraud ControlsGAO-15-665T: Published: Jun 2, 2015. Publicly Released: Jun 2, 2015.
Jun 1, 2015
VA Health Care:Actions Needed to Improve Monitoring and Oversight of Non-VA and Contract CareGAO-15-654T: Published: Jun 1, 2015. Publicly Released: Jun 1, 2015.
Medicare Administrative Contractors:CMS Should Consider Whether Alternative Approaches Could Enhance Contractor PerformanceGAO-15-372: Published: Apr 30, 2015. Publicly Released: Jun 1, 2015.
Patient Protection and Affordable Care Act:Despite Some Delays, CMS Has Made Progress Implementing Programs to Limit Health Insurer RiskGAO-15-447: Published: Apr 30, 2015. Publicly Released: Jun 1, 2015.
Private Health Insurance:Premiums and Enrollment for New Nonprofit Health Insurance Issuers Varied Significantly in 2014GAO-15-304: Published: Apr 30, 2015. Publicly Released: Jun 1, 2015.
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A portrait can sometimes help us see people in new and revealing ways. KSJD's Tom Yoder talks with local artist Bronwyn Strickland, who has painted portraits of homeless people who have been guests at the Bridge Emergency Shelter in Cortez, and Laurie Knutson, Executive Director at the Bridge, about how portraits and stories of homeless folks help us connect with them, and the upcoming fundraising event "Faces of Folk", a celebration of the lives of homeless people in our community.
With continuing drought across the Four Corners area, it is critical that we work to protect our homes, neighborhoods, and communities from the devastation of wildfire. KSJD's Tom Yoder talks with Rebecca Samulski, Montezuma Chapter Coordinator for FireWise of Southwest Colorado, about how you can be better prepared for wildfire, and join in events happening on National Wildfire Community Preparedness Day on May 2nd.
Sexual trauma and harassment in the military is an issue that can take many forms. In this episode of KSJD's monthly look at veterans affairs, KSJD's Tom Yoder talks with Bert Valencia and Darla Sanders from local VFW Post 5231 and DAV Chapter 44, and Sandi Valencia, a woman veteran who served in the Army during the Invasion of Kuwati, about how women and men confront sexual trauma in the ranks of the military, and what can be done about it. Sandi Valencia also shares a poem she wrote about serving as a woman in the military, and it is also available below as a separate audio file.
The Jefferson County Open School in Lakewood, Colorado provides alternatives to conventional schooling that strive toward creating lifelong learners.
Ensuring that children have a strong and healthy start in life is critical to their future success. KSJD's Tom Yoder talks with Geof Byerly and Vangi McCoy from the Montelores Early Childhood Council about early child health and development, resources available locally to help families provide for their children at an early age, and how the new documentary film "The Raising of America" explores the root causes of class and racial inequities in the health and well being of our kids.
In this episode of Pandora's Box, host Lyn Patrick talks with Mike Eisenfeld, New Mexico Energy Coordinator with the San Juan Citizens Alliance, about a methane gas concentration that appeared over the Four Corners area in NASA satellite images, and discuss the environmental and public health implications of methane, how leading scientists are researching the source of the methane, and what concerned citizens can do.
The City of Cortez and Heart and Soul Cortez are offering some opportunities for some spring cleaning and for getting to know your neighbors. KSJD's Tom Yoder talks with Cortez City Manager Shane Hale and Rocky Moss, the Project Coordinator for Heart and Soul Cortez, about city clean-up events happening in late April, and how neighborhoods can get funding through Heart and Soul Cortez to host a block party to help build and strengthen community values.
The rights of victims of crime are important to ensure that they have certain information, protections, and a limited role in the criminal justice process. KSJD's Tom Yoder talks with Leslie, the Victim Compensation Administrator at the 22nd Judicial District, and Gene Peck from Southwest Intertribal Voice, about what rights victims of crimes have, how they exercise those rights, and why it is important for communities to understand victims' rights and commemorate them.
Administration of a public school system provides a perspective into education that can reveal important issues. KSJD's newest interview host Dave Hart talks with Montezuma-Cortez School Superintendent Alex Carter about why a shortage of teachers could be a crisis in coming years, how poverty is affecting education in America, and opportunities that the new MCHS high school will bring to students.
In this episode of Talking Heads, KSJD's Jeff Pope and Gail Binkly from the Four Corners Free Press sit down with Bob Bragg, agriculture educator and co-host of KSJD's Ag Markets & More program, to discuss the outlook for water for the upcoming growing season, and what it means for Montezuma County producers who rely on irrigation, and for consumers.


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Casa Xochitl Great Space For Lots of Baja Living!
From $90 / night
Description from the owner
Description from the owner
Bedrooms: 2/
Bathrooms: 2/
Sleeps: 4/
Type: Bed and Breakfast
Casa Xochitl is a guesthouse for world travelers and those just beginning to discover new horizons. The ideal place for individuals and couples interested in experiencing down to earth no-nonsense Baja culture, maybe learning some Spanish and exploring the desert, sierra and solitary beaches. Casa Xochitl is an airy living environment saturated with natural, hand-crafted Mexican charm on the edge of a rustic and laid back desert village, minutes from beaches and La Paz center.
all utilities, 9MB/sec on 2.4Ghz WIFI, laundry room, separate art studio - work space
large open foyer with library area & desert garden, seating for up to 10 dinner guests.
Tangerine ...Read more
About the owner
About the owner
Casa Xochitl
Casa Xochitl
- Response rate: 95%
- Response time: Within a day
- Calendar updated: 4 days ago
Overall rating:
4.3 / 5.0
Casa Xochitl Great Space For Lots of Baja Living! La Paz, Baja California, Mexico
Additional Location Information
Do you appreciate a down to earth atmosphere, human quality and open space created with handmade materials? We are world travelers and have hosted many interesting guests on their way up or down the peninsula, east or west around the planet or momentarily escaping from their respective race tracks.
More About This Location
Mountain nearby, Rural retreats, Warm winter getaways
Getting There
Nearest ferry port: Pichilingue, 20 miles
Nearest airport: LAP, 4 miles
Car is recommended
Show rates details
Also consider
- FlipKey has rentals in over 11,000 destinations around the world:
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- Panama City Beach condos - 1,808 rentals from $90/night
- Outer Banks vacation rentals - 2,948 rentals from $127/night


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In Part 1 of this series we moved the first steps into building our Sales Dashboard in R. In this Part 2 we explore additional ways to display sales related data. If you haven't read Part 1, it is highly … Continue reading →
In Part 1 of this series we moved the first steps into building our Sales Dashboard in R. In this Part 2 we explore additional ways to display sales related data. If you haven't read Part 1, it is highly … Continue reading →
This is a follow-up blog-post to an earlier introductory post by Steven Brey: Using R: Working with Geospatial Data. In this post, we'll learn how to plot geospatial data in ggplot2. Why might we want to do this? Well, it's really … read more ...
ggplot2 tries to make it super easy to add country or state borders to your map, and for the most part it works great as long as you include the entire map region in your plot (all the states or the entire world map for example). A long standing issue with the ggplot2 borders() function
In this post you will learn how to: Create your own quasi-shape file Plot your homemade quasi-shape file in ggplot2 Add an external svg/ps graphic to a plot Change a grid grob's color and alpha *Note get simple .md version … Continue reading →
In a previous post on my personal blog about creating Pivot Tables in R with melt and cast we covered a simple way to generate sales reports and summary tables from a data set consisting of orders. It is often said that a picture is … Continue reading →
Blog post: a very basic introduction to building data plots using ggplot2 in R. #Rstats
Ah, the barplot. Loved by some, hated by some, the first graph you're likely to make in your favourite office spreadsheet software, but a rather tricky one to pull off in R. Or, that depends. If you just need a barplot that displays the value of each data point as a bar — which is
A new ggplot2-based implementation of control charts for the #RStats package qcc, and some lessons learned along the way.
I recently got an email from a person at Packt publishing, who suggested I write a book for them about ggplot2. My answer, which is perfectly true, is that I don't have the time, nor the expertise to do that. What I didn't say is that 1) a quick web search suggests that Packt doesn't
In the third and last of the ggplot series, this post will go over interesting ways to visualize the distribution of your data.


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Videos: Newscasts
AFN Pacific Update: LS3 MitchellVideo by Seaman Krystina Coffey | American Forces Network Pacific | Date: 07.01.2015
Joining the service is a commitment to be adaptable, changing duty stations every couple years, learning different jobs, and finding new friends with every move. Seaman Krystina Coffey finds out how a Sailor finds something permanent while serving overseas. View Video
DoD News: SECDEF Announces Presidential Nominee for USMC CommandantCourtesy Video | DoD News | Date: 07.01.2015
℠2015 – Secretary of Defense Ash Carter along with Secretary of the Navy Ray Mabus and Chairman of the Joint Chiefs of Staff General Martin Dempsey announced their recommendation and the President's nomination for Commandant of the Marine Corps, Lieutenant General Bob Neller. View Video
Air Force Report: Fire MusterVideo by Staff Sgt. Traci Keller | Defense Media Activity - Air Force | Date: 07.01.2015
On today's Air Force Report Staff Sergeant Traci Keller updates us on Operation Inherent Resolve and shows us how one airman experiences physical challenges associated with firefighting. View Video
DoD News: Feds Feed FamiliesCourtesy Video | DoD News | Date: 07.01.2015
2015 – A DoD News Web Update highlighting the defense.gov Special Reports page announcing the 7th annual Feds Feed Families campaign. The program, led by the U.S Department of Agriculture, was established in 2009 and is designed to help combat the shortages of food supplies in local food banks nationwide during the... View Video
All Hands Update: Career Intermission ProgramVideo by Kevin Dawson | All Hands Update | Date: 07.01.2015
Sailors may take up to three years off to pursue personal and professional goals. View Video


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Tolt Service Group Account Support Interview Questions
Interview Experience
Interview Experience
Getting an Interview
Getting an Interview
Interview Difficulty
Interviews for Top Jobs at Tolt Service Group
- Regional Service Technician II (2)
- Technician (1)
- Regional Service Technician I (1)
- Repair Teechnician (1)
- Test/Triage Tech (1)
- Account Manager (1)
- Account Support (1)
- Field Service Representative (1)
1 Candidate Interview Review Back to all InterviewsSort: Popular Date Difficulty
Account Support Interview
I applied in-person. The process took 1 day – interviewed at Tolt Service Group (Charlotte, NC) in May 2011.
This company always has someone in mind before even posting a job. It's a waste of time to even apply. Told I was moving to the next level, then never heard from them again.
Proven experience and abilities, a willingness to learn, proven leadership skills along with great communication skills are not enough to sway HR once they have in mind who they want to hire.
Interview Questions
- What would you do if....type questions Answer Question


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Jump to content
|Last action by
|The Big Wrestling Thread! in The Sporting Arena
|Windows 10 Insider Preview in Microsoft Beta (Windows 10)
|GPU Hierarchy Chart - June 2015 (w/ UK prices) in Hardware Hangout
|Found fossil skull, no idea what it is? in General Discussion
|Louisiana won't Recognize Same-Sex Marriages in Real World News
Posted 29 November 2012 - 13:28
Posted 29 November 2012 - 14:10
What stops Google Drive Email system from becoming the next megaupload just with emails?
Posted 29 November 2012 - 14:22
Not sure, but RIAA hasn't gone after Google about Google music where you upload your own music files, under the agreement you agree to about only uploading files you legally have rights to.
Posted 30 November 2012 - 10:31
I wouldn't worry about it, just use the thing until it becomes a problem then move onto the next thing!
Does this actually worry you? I'm pretty sure Google has given this plenty of thought, it's not really anyone's problem except Google's.
Posted 30 November 2012 - 10:45
Actually it does. If i'll share something google won't like and the'll suspend my account like dropbox did.. losing my gmail account .. I'm getting the chills just from thinking about it.


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Dale Tiffany Mission Wall Sconce - 15W in. BronzeItem # HN-DY343
- Classic design and modern artistry for a transitional look
- Mica bronze finish adds warmth and character
- Triangular Tiffany glass shade
- 15W x 6.5H inches
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Item was fine, but a wall sconce is a hard wired item. I got one with a plug? I had to have the electrician make the wiring work.
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