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Publications (171)598.39 Total impact
Article: Anti-inflammatory activity of SMP30 modulates NF-κB through protein tyrosine kinase/phosphatase balance[Show abstract] [Hide abstract]
ABSTRACT: Recent studies on senescence marker protein-30 (SMP30) have shown that it has an important functional role in the aging process, but its precise participation in cellular works has not been fully determined. We hypothesize that SMP30 plays crucial roles in signaling processes by modulating the balance of protein tyrosine kinase (PTK)/protein tyrosine phosphatase (PTP) and in activating proinflammatory NF-κB. An experimental paradigm of gain and loss of SMP30 function was established using SMP30-overexpressed YPEN-1 cells (herein referred to as "SMP30(+) cells") and SMP30 (Y/-) knockout mouse kidneys. The resulting data show that SMP30 expression suppressed oxidative stress-induced PTK/PTP dysregulation and PP1/2A inactivation in SMP30(+) cells, leading to the suppression of NF-κB activation. In the kidneys of SMP30 (Y/-) mice, SMP30 deficiency was found to induce NF-κB activation via the upstream signaling of NIK/IKK and MAPKs and to upregulate downstream NF-κB-responsive gene expression. In this study, we also demonstrate for the first time that SMP30 deficiency induced PTK activity in SMP30 (Y/-) kidneys, thereby significantly increasing the tyrosine phosphorylation of a catalytic subunit of PP2A (PP2Ac-Tyr307). Based on these findings, we propose that SMP30 involves NF-κB regulation through the PTK/PTP balance and that the age-related decrease of SMP30 causes NF-κB activation, which contributes to an exacerbation of the inflammatory process during aging.Journal of Molecular Medicine 11/2014; 93(3). DOI:10.1007/s00109-014-1219-1 · 4.74 Impact Factor
Article: Age-associated decrease of senescence marker protein-30/gluconolactonase in individual mouse liver cells: Immunohistochemistry and immunofluorescence: Age-associated decrease of SMP30/GNL in liver[Show abstract] [Hide abstract]
ABSTRACT: AimSenescence marker protein-30 (SMP30)/gluconolactonase (GNL) is an age-associated protein in that its presence decreases with aging. Here, we used immunohistochemical analysis to investigate the changes of SMP30/GNL in individual cells of the liver from progressively aged mice.Methods Male C57BL/6 strain mice at 1, 3, 6, 12, 24 and 30 months-of-age were the source of hepatic cells used to detect SMP30/GNL. Liver sections from these mice were subjected to immunohistochemical staining with anti-SMP30/GNL antibody. For immunofluorescent staining, primary cultured hepatocytes from mice at various ages were stained with SMP30/GNL and albumin.ResultsIn liver cells from mice of all ages, SMP30/GNL staining appeared in some but not all parenchymal cells, and localized in both the nuclei and cytoplasm. Moreover, SMP30/GNL-positive staining of parenchymal cells was present only around central vein areas, but not at sites of portal veins. Furthermore, the number of SMP30/GNL-positive cells increased as mice aged from 1 to 12 months, then decreased from the 12th to 24th month. Results were similar in primary cultured hepatocytes from mice of various ages.ConclusionsSMP30/GNL-positive cells localized mainly around the central veins in the livers of mice and decreased numerically with aging, although there was no age-related change in counts of albumin-positive cells. SMP30/GNL protein occupied the nuclei and cytoplasm. Therefore, nuclear SMP30/GNL protein might be a regulatory factor specific for genes whose expression governs transcription and the aging process. Geriatr Gerontol Int 2014; ●●: ●●–●●.Geriatrics & Gerontology International 11/2014; 15(6). DOI:10.1111/ggi.12347 · 1.58 Impact Factor
Article: Potato Chip Intake Increases Ascorbic Acid Levels and Decreases Reactive Oxygen Species in SMP30/GNL Knockout Mouse Tissues[Show abstract] [Hide abstract]
ABSTRACT: Potato chips (PC) contain abundant amounts of the free-radical scavenger ascorbic acid (AA) due to the rapid dehydration of potato tubers (Solanum tuberosum) that occurs during frying. To evaluate the antioxidant activity of PC, we examined reactive oxygen species (ROS) levels in tissues from SMP30/GNL knockout (KO) mice that cannot synthesize AA and determined AA and ROS levels after the animals were fed 20 and 10% PC diets for 7 weeks. Compared with AA-sufficient mice, AA-depleted SMP30/GNL KO mice showed high ROS levels in tissues. SMP30/GNL KO mice fed a PC diet showed high AA and low ROS levels in brain, heart, lung, testis, soleus muscle, plantaris muscle, stomach, small intestine, large intestine, eyeball, and epididymal fat compared with AA-depleted mice. Our data suggest that PC intake increases AA levels and enhances ROS scavenging activity in tissues of SMP30/GNL-KO mice, which are a promising model for evaluating the antioxidant activity of foods.Journal of Agricultural and Food Chemistry 09/2014; 62(38). DOI:10.1021/jf502587j · 3.11 Impact Factor
Article: Senescence marker protein-30/gluconolactonase deficiency exacerbates diabetic nephropathy through tubular injury in a mouse model of type 1 diabetes[Show abstract] [Hide abstract]
ABSTRACT: Aims/IntroductionSenescence marker protein-30 (SMP30) is abundantly expressed in renal proximal tubule cells, but its expression decreases with age. Previous studies have shown that reduced SMP30 expression could contribute to aging-associated deterioration of cellular function and tissue injury. In the present study, we investigated the effects of SMP30 deficiency on the pathogenesis of diabetic nephropathy.Materials and Methods Diabetes was induced using streptozotocin in male SMP30 knockout mice (KO) and wild-type mice at 7 weeks-of-age. Vitamin C was added to the drinking water to prevent vitamin C deficiency in KO mice. The mice were killed 12 weeks after the induction of diabetes.ResultsUrinary biomarkers for proximal tubule damage were significantly increased in non-diabetic KO mice compared with wild-type mice. Furthermore, diabetes-induced tubular damage was significantly exacerbated by SMP30 deletion. Morphological analysis showed a link between cortical tubulointerstitial fibrosis area and the degree of tubular damage. However, SMP30 deletion did not affect mesangial expansion. Tubular injury was associated with accumulation of hypoxia-inducible factor-1α and increased hypoxia-inducible factor-1α targeted gene expression. SMP30 deletion initiated oxidative stress; however, it did not exacerbate the oxidative stress seen in diabetic mice. In contrast, tubular inflammation was associated with SMP30 deletion only in diabetic mice.Conclusions Based on this evidence, we concluded that SMP30 deficiency exacerbates proximal tubule injury in diabetic mice. Decreased SMP30 could contribute to the increased incidence of various chronic kidney diseases, including diabetic nephropathy, with age.07/2014; 6(1). DOI:10.1111/jdi.12252
Article: Ascorbic acid deficiency affects genes for oxidation-reduction and lipid metabolism in livers from SMP30/GNL knockout mice.[Show abstract] [Hide abstract]
ABSTRACT: We sought to elucidate the effect of an ascorbic acid (AA) deficiency on gene expression, because the water soluble antioxidant AA is an important bioactive substance in vivo. We performed microarray analyses of the transcriptome in the liver from senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which are unable to synthesize AA in vivo. Our microarray analysis revealed that the AA deficiency increased gene expression related to the oxidation-reduction process, i.e., the nuclear factor, erythroid derived 2, like 2 (Nrf2) gene, which is a reactive oxygen species-sensitive transcriptional factor. Moreover, this AA deficiency increased the expression of genes for lipid metabolism including the cytochrome P450, family 7, subfamily a, polypeptide 1 (Cyp7a1), which is a late-limiting enzyme of the primary bile acid biosynthesis pathway. Although an AA deficiency increased the Cyp7a1 protein level, bile acid levels in the liver and gallbladder decreased. Since Cyp7a1 has a heme iron at the active site, AA must function as a reductant of the iron required for the continuous activation of Cyp7a1. This experimental evidence strongly supports a role for AA in the physiologic oxidation-reduction process and lipid metabolism including bile acid biosynthesis. General significance Although many effects of AA supplementation have been reported, no microarray analysis of AA deficiency in vivo is available. Results from using this unique model of AA deficiency, the SMP30/GNL-KO mouse, now provide new information about formerly unknown AA functions that will implement further study of AA in vivo.Biochimica et Biophysica Acta 04/2014; DOI:10.1016/j.bbagen.2014.03.019 · 4.66 Impact Factor
Article: Susceptibility QTLs for pathogenic leukocytosis in SCG/Kj mice, a spontaneously occurring crescentic glomerulonephritis and vasculitis model[Show abstract] [Hide abstract]
ABSTRACT: Background The spontaneous crescentic glomerulonephritis-forming/Kinjoh (SCG/Kj) mouse, a model of human crescentic glomerulonephritis (CrGN) and systemic vasculitis, is characterized by the production of MPO-ANCA and marked leukocytosis. This study was performed to identify the specific populations of leukocytes associated with CrGN and susceptibility loci for pathogenic leukocytosis.Methods420 female (C57BL/6 × SCG/Kj) F2 intercross mice were subjected to serial flow cytometry examination of the peripheral blood (PB). Kidney granulocytes and monocytes were histopathologically examined. Linkage analyses were done with 109 polymorphic microsatellite markers.ResultsCorrelation studies revealed that increase of the granulocytes, F4/80+ cells, CD3+CD4-CD8- T cells, and dendritic cells (DCs) in PB were significantly associated with glomerulonephritis, crescent formation and vasculitis. In kidney sections, F4/80low cells were observed in crescent, while F4/80high cells were around the Bowman's capsules and in the interstitium. Numbers of F4/80+ cells in crescents significantly correlated with F4/80+ cell numbers in PB, but not with numbers of F4/80+ cells in the interstitium. Genome-wide quantitative trait locus (QTL) mapping revealed three SCG/Kj-derived non-Fas QTLs for leukocytosis, two on chromosome 1 and one on chromosome 17. QTLs on chromosome 1 affected DCs, granulocytes and F4/80+ cells, but QTL on chromosome 17 affected DCs and granulocytes.Conclusion We found CrGN-associated leukocytes and susceptibility QTLs with their positional candidate genes. F4/80+ cells in crescents are considered as recruited inflammatory macrophages. The results provide information for leukocytes to be targeted and genetic elements in CrGN and vasculitis.Clinical & Experimental Immunology 03/2014; 177(1). DOI:10.1111/cei.12333 · 3.28 Impact Factor
Article: Senescence marker protein-30/superoxide dismutase 1 double knockout mice exhibit increased oxidative stress and hepatic steatosis[Show abstract] [Hide abstract]
ABSTRACT: Superoxide dismutase 1 (SOD1) is an antioxidant enzyme that converts superoxide anion radicals into hydrogen peroxide and molecular oxygen. The senescence marker protein-30 (SMP30) is a gluconolactonase that functions as an antioxidant protein in mammals due to its involvement in ascorbic acid (AA) biosynthesis. SMP30 also participates in Ca2+ efflux by activating the calmodulin-dependent Ca2+-pump. To reveal the role of oxidative stress in lipid metabolism defects occurring in non-alcoholic fatty liver disease pathogenesis, we generated SMP30/SOD1-double knockout (SMP30/SOD1-DKO) mice and investigated their survival curves, plasma and hepatic lipid profiles, amounts of hepatic oxidative stress, and hepatic protein levels expressed by genes related to lipid metabolism. While SMP30/SOD1-DKO pups had no growth retardation by 14 days of age, they did have low plasma and hepatic AA levels. Thereafter, 39% and 53% of male and female pups died by 15–24 and 89 days of age, respectively. Compared to wild type, SMP30-KO and SOD1-KO mice, by 14 days SMP30/SOD1-DKO mice exhibited: (1) higher plasma levels of triglyceride and aspartate aminotransferase; (2) severe accumulation of hepatic triglyceride and total cholesterol; (3) higher levels of superoxide anion radicals and thiobarbituric acid reactive substances in livers; and (4) decreased mRNA and protein levels of Apolipoprotein B (ApoB) in livers – ApoB is an essential component of VLDL secretion. These results suggest that high levels of oxidative stress due to concomitant deficiency of SMP30 and/or AA, and SOD1 cause abnormal plasma lipid metabolism, hepatic lipid accumulation and premature death resulting from impaired VLDL secretion.01/2014; 4. DOI:10.1016/j.fob.2014.05.003
Article: Ascorbic acid prevents protein oxidation in livers of senescence marker protein-30/gluconolactonase knockout mice[Show abstract] [Hide abstract]
ABSTRACT: Senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice are incapable of synthesizing L-ascorbic acid (AA) in vivo. As AA is known to be a water-soluble anti-oxidant, we assessed protein oxidation levels in livers from SMP30/GNL KO mice maintained in an AA-insufficient condition. Livers were collected from male SMP30/GNL KO mice at the ages of 3, 6 and 12 months, and wild-type (WT) mice at the ages of 3, 6, 12 and 24 months. To assess protein oxidation, we measured the content of protein carbonyl, which is a major protein oxidation marker. AA levels were measured by 2,4-dinitrophenylhydrazine method using high-performance liquid chromatography. Livers of SMP30/GNL KO mice had just ∼5% as much AA as those of WT mice from 3 to 12 months-of-age. Protein carbonyl levels in livers from SMP30/GNL KO mice were a significant 1.8- to 2.3-fold higher than those from age-matched WT mice. To establish that the AA-insufficiency caused this difference, we added AA to some drinking water, and examined the effect on AA and protein carbonyl levels in livers from SMP30/GNL KO and WT mice. Livers from SMP30/GNL KO mice given extra AA had a significantly higher content than those from their deprived counterparts. Furthermore, protein carbonyl levels in livers from AA-supplemented SMP30/GNL KO mice were significantly lower than those from the SMP30/GNL KO mice without AA supplementation. However, added AA did not affect the protein carbonyl levels in WT mice. These results strongly suggest that AA plays an important role in preventing protein oxidation in vivo, thus enhancing overall health. Geriatr Gerontol Int 2013; ●●: ●●-●●.Geriatrics & Gerontology International 10/2013; 14(4). DOI:10.1111/ggi.12162 · 1.58 Impact Factor
Article: Vitamin C Prevents Cigarette Smoke-Induced Pulmonary Emphysema in Mice and Provides Pulmonary Restoration[Show abstract] [Hide abstract]
ABSTRACT: Rationale: Vitamin C (VC) is a potent antioxidant and essential for collagen synthesis. Objectives: We investigated whether VC-treatment prevents and cures smoke-induced emphysema in senescence marker protein-30 knockout (SMP30-KO) mice, which cannot synthesize VC. Methods: Two smoke-exposure experiments using SMP30-KO were conducted. In the first one (a preventive study), four-month-old mice were received minimal VC (0.0375 g/L) [VC(L)] or physiologically sufficient VC (1.5 g/L) [VC(S)], then were exposed to cigarette smoke or smoke-free air for 2 months. Pulmonary evaluations followed when the mice were 6-months of age. The second, study began after the establishment of smoke-induced emphysema (a treatment study). These mice no longer underwent smoke exposure but received VC(S) or VC(L) treatment for 2 months. Morphometric analysis and measurements of oxidative stress, collagen synthesis, and vascular endothelial growth factor (VEGF) in the lungs were evaluated. Measurements and Main Results: Chronic smoke exposure caused emphysema [29.6% increases of mean linear intercepts (MLI) and 106.5% increases of destructive index (DI) compared with the air-only group] in 6-month-old SMP30-KO mice and this emphysema closely resembled human COPD. Furthermore, smoke-induced emphysema persisted in the VC(L) group after smoking cessation, whereas VC-treatment provided pulmonary restoration [18.5% decrease of MLI and 41.3% decrease of DI compared with VC(L) group]. VC-treatment diminished oxidative stress, increased collagen synthesis, and improved VEGF levels in the lungs. Conclusions: Our results suggest that VC not only prevents smoke-induced emphysema in SMP30-KO mice but also restores emphysematous lungs. Therefore, VC may provide a new therapeutic strategy for treating COPD in humans.American Journal of Respiratory Cell and Molecular Biology 09/2013; 50(2). DOI:10.1165/rcmb.2013-0121OC · 4.11 Impact Factor
Article: Lepr(db/db) Mice with Senescence Marker Protein-30 Knockout (Lepr(db/db)Smp30(Y/-) ) Exhibit Increases in Small Dense-LDL and Severe Fatty Liver Despite Being Fed a Standard Diet.[Show abstract] [Hide abstract]
ABSTRACT: BACKGROUNDAIMS: The senescence marker protein-30 (SMP30) is a 34 kDa protein originally identified in rat liver that shows decreased levels with age. Several functional studies using SMP30 knockout (Smp30(Y/-) ) mice established that SMP30 functions as an antioxidant and protects against apoptosis. To address the potential role of SMP30 in nonalcoholic fatty liver disease (NAFLD) pathogenesis, we established Smp30(Y/-) mice on a Lepr(db/db) background (Lepr(db/db)Smp30(Y/-) mice). RESEARCH DESIGNPRINCIPAL FINDINGS: Male Lepr(db/db)Smp30(Y/-) mice were fed a standard diet (340 kcal/100 g, fat 5.6%) for 16 weeks whereupon the lipid/lipoprotein profiles, hepatic expression of genes related to lipid metabolism and endoplasmic reticulum stress markers were analyzed by HPLC, quantitative RT-PCR and western blotting, respectively. Changes in the liver at a histological level were also investigated. The amount of SMP30 mRNA and protein in livers was decreased in Lepr(db/db)Smp30(Y/+) mice compared with Lepr(db/+)Smp30(Y/+) mice. Compared with Lepr(db/db)Smp30(Y/+) mice, 24 week old Lepr(db/db)Smp30(Y/-) mice showed: i) increased small dense LDL-cho and decreased HDL-cho levels; ii) fatty liver accompanied by numerous inflammatory cells and increased oxidative stress; iii) decreased mRNA expression of genes involved in fatty acid oxidation (PPARα) and lipoprotein uptake (LDLR and VLDLR) but increased CD36 levels; and iv) increased endoplasmic reticulum stress. Our data strongly suggest that SMP30 is closely associated with NAFLD pathogenesis, and might be a possible therapeutic target for NAFLD.PLoS ONE 06/2013; 8(6):e65698. DOI:10.1371/journal.pone.0065698 · 3.53 Impact Factor
Article: Exome sequencing of senescence-accelerated mice (SAM) reveals deleterious mutations in degenerative disease-causing genes[Show abstract] [Hide abstract]
ABSTRACT: Background Senescence-accelerated mice (SAM) are a series of mouse strains originally derived from unexpected crosses between AKR/J and unknown mice, from which phenotypically distinct senescence-prone (SAMP) and -resistant (SAMR) inbred strains were subsequently established. Although SAMP strains have been widely used for aging research focusing on their short life spans and various age-related phenotypes, such as immune dysfunction, osteoporosis, and brain atrophy, the responsible gene mutations have not yet been fully elucidated. Results To identify mutations specific to SAMP strains, we performed whole exome sequencing of 6 SAMP and 3 SAMR strains. This analysis revealed 32,019 to 38,925 single-nucleotide variants in the coding region of each SAM strain. We detected Ogg1 p.R304W and Mbd4 p.D129N deleterious mutations in all 6 of the SAMP strains but not in the SAMR or AKR/J strains. Moreover, we extracted 31 SAMP-specific novel deleterious mutations. In all SAMP strains except SAMP8, we detected a p.R473W missense mutation in the Ldb3 gene, which has been associated with myofibrillar myopathy. In 3 SAMP strains (SAMP3, SAMP10, and SAMP11), we identified a p.R167C missense mutation in the Prx gene, in which mutations causing hereditary motor and sensory neuropathy (Dejerine-Sottas syndrome) have been identified. In SAMP6 we detected a p.S540fs frame-shift mutation in the Il4ra gene, a mutation potentially causative of ulcerative colitis and osteoporosis. Conclusions Our data indicate that different combinations of mutations in disease-causing genes may be responsible for the various phenotypes of SAMP strains.BMC Genomics 04/2013; 14(1):248. DOI:10.1186/1471-2164-14-248 · 4.04 Impact Factor
Article: Effect of ascorbic acid deficiency on catecholamine synthesis in adrenal glands of SMP30/GNL knockout mice.[Show abstract] [Hide abstract]
ABSTRACT: PURPOSE: The effect of an AA deficiency on catecholamine biosynthesis in adult mice in vivo is unknown. Therefore, we quantified catecholamine and the expression of catecholamine synthetic enzymes in the adrenal glands of senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice placed in an AA-deficient state. METHODS: At 30 days of age, mice were divided into the following 4 groups: AA (-) SMP30/GNL KO, AA (+) SMP30/GNL KO, AA (-) wild type (WT), and AA (+) WT. The AA (+) groups were given water containing 1.5 g/L AA, whereas the AA (-) groups received water without AA until the experiment ended. In addition, all mice were fed an AA-depleted diet. Catecholamine levels were measured by a liquid chromatographic method. Tyrosine hydroxylase, dopa decarboxylase, dopamine β-hydroxylase, and phenylethanolamine N-methyltransferase mRNA expression levels were measured with the quantitative real-time polymerase chain reaction (qPCR). Tyrosine hydroxylase and dopamine β-hydroxylase protein levels were quantified by Western blot analysis. RESULTS: In the adrenals of AA (-) SMP30/GNL KO mice, noradrenaline and adrenaline levels decreased significantly compared to other three groups of mice, although there were no significant differences in dopamine β-hydroxylase or phenylethanolamine N-methyltransferase mRNA content. Moreover, there was no significant difference in their dopamine β-hydroxylase protein levels. On the other hand, AA depletion did not affect dopamine levels in adrenal glands of mice. CONCLUSION: An AA deficiency decreases the noradrenaline and adrenaline levels in adrenal glands of mice in vivo.European Journal of Nutrition 03/2013; DOI:10.1007/s00394-013-0515-9 · 3.84 Impact Factor
Article: Insufficient ascorbic acid intake during gestation induces abnormal cardiac dilation in fetal and neonatal SMP30/GNL knockout mice.[Show abstract] [Hide abstract]
ABSTRACT: Background:Despite the acknowledged importance of ascorbic acid (AA) in maintaining pregnancy and normal fetal development, this vitamin's precise actions remain obscure. Therefore, we investigated the impact of maternal AA content on the growth of fetal mice during the gestation period by using senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice, which cannot synthesize AA in vivo.Methods:SMP30/GNL KO mice gave birth after a gestation period with absent, low, or normal AA intake. AA was measured by using high performance liquid chromatography and electrochemical detection. Whole body sections were stained with hematoxylin and eosin, Elastica van Gieson, and Azan.Results:The mothers absent AA intake failed to bear young at all because of incomplete fetal development. Offspring born under the low AA condition generally died within a few days after birth. Morphological analysis revealed that the latter neonates of SMP30/GLN KO mothers whose intake of AA was low during gestation manifested abnormal cardiac dilation, congestion of liver and lung, incompletely expanded pulmonary alveoli, and impaired vertebral bodies. In contrast, a normal AA diet produced healthy progeny.Conclusion:A diet sufficiently replete with AA is essential during the gestational period for normal tissue development in the fetus and neonate.Pediatric Research (2013); doi:10.1038/pr.2013.22.Pediatric Research 02/2013; DOI:10.1038/pr.2013.22 · 2.84 Impact Factor
Article: Structural Basis of the γ-Lactone-Ring Formation in Ascorbic Acid Biosynthesis by the Senescence Marker Protein-30/Gluconolactonase[Show abstract] [Hide abstract]
ABSTRACT: The senescence marker protein-30 (SMP30), which is also called regucalcin, exhibits gluconolactonase (GNL) activity. Biochemical and biological analyses revealed that SMP30/GNL catalyzes formation of the γ-lactone-ring of l-gulonate in the ascorbic acid biosynthesis pathway. The molecular basis of the γ-lactone formation, however, remains elusive due to the lack of structural information on SMP30/GNL in complex with its substrate. Here, we report the crystal structures of mouse SMP30/GNL and its complex with xylitol, a substrate analogue, and those with 1,5-anhydro-d-glucitol and d-glucose, product analogues. Comparison of the crystal structure of mouse SMP30/GNL with other related enzymes has revealed unique characteristics of mouse SMP30/GNL. First, the substrate-binding pocket of mouse SMP30/GNL is designed to specifically recognize monosaccharide molecules. The divalent metal ion in the active site and polar residues lining the substrate-binding cavity interact with hydroxyl groups of substrate/product analogues. Second, in mouse SMP30/GNL, a lid loop covering the substrate-binding cavity seems to hamper the binding of l-gulonate in an extended (or all-trans) conformation; l-gulonate seems to bind to the active site in a folded conformation. In contrast, the substrate-binding cavities of the other related enzymes are open to the solvent and do not have a cover. This structural feature of mouse SMP30/GNL seems to facilitate the γ-lactone-ring formation.PLoS ONE 01/2013; 8(1):e53706. DOI:10.1371/journal.pone.0053706 · 3.53 Impact Factor
Article: Senescence Marker Protein-30/Gluconolactonase Expression in the Mouse Ovary during Gestation.[Show abstract] [Hide abstract]
ABSTRACT: Senescence marker protein-30 (SMP30) was first described as a physiologic entity that decreases in the rat liver and kidney with aging. Previously, we established that SMP30 is the lactone-hydrolyzing enzyme gluconolactonase (GNL), which is involved in ascorbic acid (AA) biosynthesis. In the present study, we found SMP30/GNL mRNA expressed in the mouse ovary. To ascertain the reason for ovarian SMP30/GNL expression, we examined mice during gestation. SMP30/GNL mRNA expression was evident at the start of gestation, increased for the next eight days then decreased rapidly. Moreover, L-gulono-γ-lactone oxidase (Gulo) mRNA, which catalyzes the last step of AA, was found in the ovaries of these mice. The variations of these genes' expression showed an inverse pattern to that of Cyp19a1 (aromatase) mRNA expression. Therefore, the SMP30/GNL and Gulo mRNA expression might be regulated by estrogen levels in the ovary. Since the presence of both SMP30/GNL and Gulo mRNAs could indicate that AA synthesis occurs in the ovary, we quantified AA levels in mouse ovaries during gestation. However, no correlation was found between changes of AA content and SMP30/GNL or Gulo mRNAs expression at this site. Moreover, we compared the changes of AA content during gestation between wild-type and SMP30/GNL knockout mice, which cannot synthesize AA, and found no significant differences between them. These results indicated that, although AA synthesis might occur in the ovaries, the amount of AA which is synthesized in ovaries must be quite low and insufficient to influence the AA content in ovary.Biological & Pharmaceutical Bulletin 01/2013; 36(12):2005-8. DOI:10.1248/bpb.b13-00309 · 1.78 Impact Factor
Article: Effects of Ascorbic Acid Deficiency on Protein and Lipid Oxidation in Livers from SMP30/GNL Knockout Mice.[Show abstract] [Hide abstract]
ABSTRACT: Ascorbic acid (AA) functions as an electron donor and scavenges reactive oxygen species such as superoxide, singlet oxygen, and hydroxyl radicals in vitro. However, little is known about the effect of an AA deficiency on protein and lipid oxidation levels in the liver. Therefore, we measured the levels of protein carbonyl and thiobarbituric acid reactive substances (TBARS) in livers from senescence marker protein-30 (SMP30)/gluconolactonase (GNL) knockout (KO) mice. These mice are deficient in AA, because they lack the SMP30/GNL gene, which is essential for the biosynthesis of AA in vivo. To track the effect of an AA deficiency, at 30 d of age, mice were divided into the following four groups: AA (-) SMP30/GNL KO, AA (+) SMP30/GNL KO, AA (-) wild type (WT), and AA (+) WT. The AA (+) groups were given water containing 1.5 g/L AA, whereas the AA (-) groups received water without AA for 57 d. All mice were fed an AA-free diet. Subsequently, protein carbonyl levels in livers from AA (-) SMP30/GNL KO mice were significantly higher than those from the other three groups; however, TBARS levels were not significantly different among the four groups. Therefore, AA must act as an anti-oxidant for proteins but might not directly protect lipid oxidation in the liver.Journal of Nutritional Science and Vitaminology 01/2013; 59(6):489-95. DOI:10.3177/jnsv.59.489 · 0.87 Impact Factor
Article: Ascorbic acid enhances the expression of type 1 and type 4 collagen and SVCT2 in cultured human skin fibroblasts[Show abstract] [Hide abstract]
ABSTRACT: Ascorbic acid (AA) is essential for collagen biosynthesis as a cofactor for prolyl and lysyl hydroxylase and as a stimulus for collagen gene expression. Many studies have evaluated the relationship between AA and collagen expression in short- and long-term effects on cells after a single administration of AA into the culture medium. However, no such study has monitored in detail the stability of AA in medium or the alterations of intracellular AA levels during a protracted interval. Therefore, we examined here intracellular AA levels and stability throughout its exposure to human skin fibroblasts in vitro. Moreover, we determined the effects on type 1 and type 4 collagen and sodium-dependent vitamin C transporter (SVCT) gene expression when medium containing 100 μM AA was replaced every 24 hours for 5 days to avoid depletion of AA. Throughout this long-term culture, intracellular AA levels remained constant; the expression of type 1 and type 4 collagens and SVCT2 mRNA was enhanced, and type 1 procollagen synthesis increased. Thus, these results indicate that human skin fibroblasts exposed to AA over time had rising levels of type 1 / type 4 collagens and SVCT2 mRNA expression and type 1 procollagen synthesis.Biochemical and Biophysical Research Communications 12/2012; 430(2). DOI:10.1016/j.bbrc.2012.11.110 · 2.28 Impact Factor
Article: Peptidylarginine deiminase and protein citrullination in prion diseases: Strong evidence of neurodegeneration[Show abstract] [Hide abstract]
ABSTRACT: The post-translational citrullination (deimination) process is mediated by peptidylarginine deiminases (PADs), which convert peptidylarginine into peptidylcitrulline in the presence of high calcium concentrations. Over the past decade, PADs and protein citrullination have been commonly implicated as abnormal pathological features in neurodegeneration and inflammatory responses associated with diseases such as multiple sclerosis, Alzheimer disease and rheumatoid arthritis. Based on this evidence, we investigated the roles of PADs and citrullination in the pathogenesis of prion diseases. Prion diseases (also known as transmissible spongiform encephalopathies) are fatal neurodegenerative diseases that are pathologically well characterized as the accumulation of disease-associated misfolded prion proteins, spongiform changes, glial cell activation and neuronal loss. We previously demonstrated that the upregulation of PAD2, mainly found in reactive astrocytes of infected brains, leads to excessive citrullination, which is correlated with disease progression. Further, we demonstrated that various cytoskeletal and energy metabolism-associated proteins are particularly vulnerable to citrullination. Our recent in vivo and in vitro studies elicited altered functions of enolase as the result of citrullination; these altered functions included reduced enzyme activity, increased protease sensitivity and enhanced plasminogen-binding affinity. These findings suggest that PAD2 and citrullinated proteins may play a key role in the brain pathology of prion diseases. By extension, we believe that abnormal increases in protein citrullination may be strong evidence of neurodegeneration.Prion 09/2012; 6(5). DOI:10.4161/pri.22380 · 1.97 Impact Factor
Article: Two novel sandwich ELISAs identify PAD4 levels and PAD4 autoantibodies in patients with rheumatoid arthritis.[Show abstract] [Hide abstract]
ABSTRACT: OBJECTIVE: The peptidylarginine deiminase 4 (PAD4) gene and PAD4 autoantibodies have been associated with rheumatoid arthritis (RA) and its pathogenesis. Therefore, methods for accurately determining their levels in the peripheral blood of these patients would be a diagnostic asset. The objective of our study was to adapt the enzyme-linked immunosorbent assay (ELISA) method for evaluating PAD4 levels in human blood. METHODS: We prepared recombinant human (h)PAD1, -2, -3, and -4 proteins to develop mouse monoclonal antibodies specific to hPAD4. We then generated six monoclonal antibodies against hPAD4 and developed two new sandwich ELISA methods for evaluating hPAD4 and PAD4 autoantibodies in the peripheral blood from 32 patients with RA, ten patients with osteoarthrosis, and 20 healthy individuals. RESULTS: The distribution of hPAD4 in the patients' plasma was determined. Two populations were identified: one group with high hPAD4 levels (>0.57 ng/mL) and a second group with near-zero levels (<0.1 ng/mL). Most patients approximating zero hPAD4 levels had PAD4 autoantibodies. In contrast, most of those with higher plasma hPAD4 levels did not have detectable PAD4 autoantibodies. CONCLUSION: The combination of these sandwich ELISA methods may be a potentially beneficial clinical tool for diagnosing RA.Modern Rheumatology 09/2012; 23(4). DOI:10.1007/s10165-012-0748-0 · 2.21 Impact Factor
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ABSTRACT: Aim: Catecholamines, which are physiologically important neurotransmitters and hormones, apparently decrease in the brain and plasma as some species age. Because this observation has engendered controversy, we used mice to investigate whether age-related changes occur in adrenal catecholamine levels and in the expression of catecholamine synthetic enzymes. Methods: Adrenal glands were collected from male C57BL/6NCr mice at the ages of 6, 12 and 24 months. Catecholamines, such as dopamine (DA), noradrenaline (NA) and adrenaline (AD) from those glands, were measured by using a highly sensitive liquid chromatographic method with peroxyoxalate chemiluminescence reaction detection. Tyrosine hydroxylase (TH), dopa decarboxylase, dopamine beta hydroxylase (DBH) and phenylethanolamine N-methyltransferase (PNMT) mRNA expression levels were measured by quantitative real-time polymerase chain reaction. Results: Although DA levels in the adrenals of 24-month-old mice were higher than in 6- and 12-month-old mice, the AD content decreased with age. In such mice, the ratio of DA to NA at 24 months was lower than at 12 months, and the ratio of NA to AD content at 24 months was significantly lower than at 6 months. The mRNA expression ratios in TH, DBH and PNMT in 24-month-old mice were all lower than in 12-month-old mice. Conclusions: These results strongly suggest that catecholamine synthesis, in general, declines with aging in the adrenal glands of mice and that AD, in particular, undergoes a significant decrease with advancing age. Geriatr Gerontol Int 2012; ••: ••-••.Geriatrics & Gerontology International 08/2012; 13(2). DOI:10.1111/j.1447-0594.2012.00929.x · 1.58 Impact Factor
Top Journals
Tokyo Metropolitan InstituteEdo, Tōkyō, Japan
Toho University
Edo, Tōkyō, Japan
- Department of Biochemistry
Tokyo Metropolitan Institute of GerontologyEdo, Tōkyō, Japan
Hallym University
Seoul, Seoul, South Korea
- College of Medicine
Tokyo Metropolitan University
Edo, Tōkyō, Japan
- Graduate School of Science and Engineering
Niigata University
Niahi-niigata, Niigata, Japan
- Division of Neurophysiology
Saitama University
Saitama, Saitama, Japan
- Faculty of Science
Kobe Pharmaceutical UniversityJapan
Juntendo University
Edo, Tōkyō, Japan
- Department of Medicine
The Jikei University School of Medicine
Tokyo, Tokyo-to, Japan
- Department of Internal Medicine
Okayama UniversityOkayama, Okayama, Japan


text:
Published: 09 March 2015
Indian auto giant reveals a six-seat SUV concept that could one day be sold Down Under
Published: 27 February 2015
In the utility segment several newcomers are challenging the established order
Foton Tunland v Mahindra Genio v Tata Xenon
Published: 02 February 2015
New Indian hatch launches in sub-continent, could be Australia bound in future
Published: 08 January 2015
Autonomous driving and updated infotainment systems on show in Tata's Elxsi connected-car concept
Published: 03 December 2014
For price-conscious commercial buyers, Tata's Xenon is doing everything right to gain a toehold in its chosen niche
Now with a four-star ANCAP safety rating and a comprehensive warranty program, the Xenon dual-cab pick-up is aggressively targeting the sub-$30,000 ute market
Published: 02 December 2014
Tata Motors Australia has unveiled a run-out deal on its MY14 Xenon LCV, with thousands slashed from the regular recommended retail price
Published: 27 November 2014
Tata reveals an auto Xenon LCV is expected Down Under within the next 12 months
Published: 23 September 2014
New retailers join the ranks as Tata rolls out ownership program and four-star ANCAP rating
Published: 08 September 2014
Stability control added to Indian ute, along with after-sales cover extended to four years
Published: 01 September 2014
Budget car makes a splash in India, but won't be coming to Australia
Published: 29 July 2014
End of financial year incentive programme on Xenon LCV is followed by a new cash-back bonus scheme expiring in September
Published: 18 July 2014
Resurrecting an old but largely unknown name takes time and commitment
Tiny people's car and seven-seat SUV vetoed; other passenger cars as long as five years away
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Got a scoop? Send us your story here!
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subset: commoncrawl


text: The Tess-One wiki last edited by jeffreyv2512 on 08/16/14 11:45PM View full history Wolverine and Captaina America encounter a commonm enemy - T.E.S.S.-One, Total Elimination of Super Soldiers was created by scientist Daniel Schumann and programmed to act as a failsafe in case the members of the Super Soldier project was abusing its powers.The robot is controled by a mutant named the Overrider who had technopathy abilities.Overrider broke into Adametco- the nation's leading manufacturer of adamantium and forced the workers there to provide TESS One with a coat of adamantium. Overrider with TESS One attacked America's Nucleur Command Center and was confronted by Captain America and Wolverine. Both heroes managed to sever the head of TESS One because the joints were not laced with adamantium. The TESS One robot was taken to a military facility in Colorado and Overrider defeated.At the end Captain America says to Wolverine he would never join a team like the Avengers, because of his behaviors.


text:
Hour of the Red God
By Richard Crompton
(Sarah Crichton Books, Hardcover, 9780374171995, 290pp.)
Publication Date: May 7, 2013
List Price: $26.00*
* Individual store prices may vary.
Shop Local
Enter your zip code below to find indies closest to you.
The Maasai believe in two gods. Enkai Narok, the Black God, is benign. Enkai Nanyokie, the Red God, is the god of anger, vengeance, and death.
Nairobi, 2007. In Africa's sprawling megacity, a small elite holds power over an impoverished, restless majority. Corruption, exploitation, and ethnic rivalry are part of everyday life. Amid claims of vote rigging and fraud, the presidential elections could be the spark that sets this city ablaze.
With chaos looming, few care about one dead prostitute. But Detective Mollel does. For Mollel is a former Maasai warrior, and the dead girl was a Maasai, too.
As he ventures from slums to skyscrapers, from suburbs to sewers, Mollel begins to realize that there is more at stake than just this murder. But even as he is forced to confront his turbulent past, he begins to doubt his warrior's instincts.
Can Mollel manage to find the killer and solve the case before the Red God consumes all?
With the sophistication of Ian Rankin and Colin Harrison, and set against the backdrop of Kenya's turbulent 2007 elections, Richard Crompton's Hour of the Red God brings Nairobi vividly to life: gritty and modern, with an extraordinary blend of tribal and urban elements. In this dark thriller, tradition and power collide, arriving at a shocking, unforgettable end. And in the Maasai hero Mollel, a new detective icon is born.
One of Publishers Weekly's Best Mystery/Thriller Books of 2013


text: Canon 6D for $1789 – Deal Alert March 8, 2013 By Eric Reagan B&H has the Canon 6D for $1789 (reg. $2099). They're back-ordered right now, but should be back in stock any day now. You can still place an order for shipment as soon as they arrive in stock. Check it out here at B&H Photo.


text:
The Feed
By Stephanie March
Foodie File
By Dara Moskowitz Grumdahl
DeRusha Eats
By Jason DeRusha
Harvest Beer Festival
Mpls.St.Paul Event
Restaurant Week
Party Patrol
By Parties Editors
The Morning After
By Tad Simons
Arts Off The Cuff
by Arts & Nightlife Editors
Ali Shops
By Allison Kaplan
Perfect Parties
By Mpls.St.Paul Magazine
ASID MN Showcase Home
Exceptional Properties
By Edina Realty
Outstanding Nurses
Fit Fest
Stephanie Wilbur Ash
Featured Promotion
Aisle File
By Emily Howald Sefton
Real Brides
By Real Brides-to-Be
Diamond Awards
Dara Moskowitz Grumdahl was born in New York City, little aware of her destiny—to eat out a lot in Minnesota. She has been writing about restaurants and wine since 1997, first as the restaurant critic for City Pages, then for Minnesota Monthly and Real Food, and now for Mpls.St.Paul Magazine. Her food writing has appeared in Gourmet, Saveur, Bon Appetit, and many other publications, (her work has appeared in six editions of the Best Food Writing in America anthology, if anyone's counting,) and her last book was Drink This: Wine Made Simple. She has been nominated 10 times for James Beard Awards, the Oscars of the food world, and has won five times. She lives in south Minneapolis with her husband and two young children, a life she says is very close to perfect, because it is rich in both Sauternes and string cheese.
Sign up for Foodie! Stay posted on the local dining scene, foodie events, and tasty recipes from Mpls.St.Paul's food and dining editor.
Brian Fredericksen makes out-of-this-world honey right here in Minnesota.
One of the big stories of 2013 developed slowly but left St. Paul transformed with the openings of solid neighborhood cafes from Highland to Lowertown to the East Side.
Todd Churchill runs one of the fastest-growing beef companies in the country because it's what God would want.
Minnesota is experiencing a bitters boom—
but you ain't seen nothing yet.
Don't be bitters! Be the hero of your holiday party by bringing one of these great gifts.
Jacquie Berglund changed the world because of what may have been the most important church basement stunt in Twin Cities history.
St. Paul's newest armory looks more dangerous than it is.
Minnesota's highly skilled Indian population is booming. Dara gives her picks for best local lunches.
Schell's Brewery is poised to go where no brewery has gone before.
Bees are dying. So are moths And every other kind of pollinator. No pollinators means no flowers, no fruit, no nuts. Is this the end of the world as we know it?
Thomasina Petrus has a button nose, dimples, jet-black curls, and the ability to make your skin go to goose bumps when she sings.
Broders' new wine bar offers a distinctive twist.
When did Minnesota acquire a great Pan-Asian fried chicken scene? Just recently. Grab your wet-naps and get crunching.
Something different seems to be crackling across the Minnesota pizza-scape.
What if we were just as creative now as we were in the '90s?
This is a very pie-intelligent town. Here are the ones you shouldn't miss this summer pie season.
The final nail in Prohibition's coffin: Behold the new local microdistillery boom.
A gravedigger's daughter mourns the passing of an era.
It turns out there aren't plenty of fish in the sea after all. Meet the Minnesota entrepreneurs and scientists who are starting fish farms to solve a problem they didn't create.
Once a diminutive food truck, Smack Shack has blossomed into a New England treasure, right in downtown Minneapolis.
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text:
Experienced MQL4 programmer
United Kingdom - Last active: 1 day ago - Tests: 13 - Portfolio: 5
MetaTrader 4 Programmer (MQL4) I'm a professional programmer with over 5 years experience in trading forex and converting / interpreting systems for Expert Advisor automation. As a Company Director (CEO) of over 15 years I always work to high standards in a completely professional and ethical manner. Being UK based, I am fully conversant with the complexities of the English language and can convey complex points in an understandable manner. All indicators, expert advisors and scripts I produce are professionally coded, tested and robust, and include the following as standard (where appropriate): - 4/5 digit broker compatibility - Automatic time zone / offset recognition - Built in error checks - Built in safety features (i.e. Closure of orders where stop loss setting fails) I have previously coded the following complex scenarios which can be readily built into future projects: - Serial number protection linked to varying expiry dates - Restriction of indicators/expert advisors to specific chart types and/or timeframes - Automatic changing of charts to correct timeframes - Lot sizing based on account risk percentages - Spreadbetting compatibility - News avoidance (eg. Stopping trades around high impact releases) - Scraping for retrieving information from web sites / RSS feeds I have developed a robust licencing system which can be encoded into any EA or indicator. It allows on-line authorization, chart and time-frame restrictions plus allows remote changing of EA/indicator settings and variables. Please ask for a demo if you are interested. All expert advisors are coded to provide for enhanced optimization and quick backtesting. As a supplementary service I can create professional looking installers and autorun front ends for installation CD's, USB sticks, etc., including the incorporation of logos and branding graphics. I also have experience writing technical manuals, creating video tutorials and preparing seminars.
Legal Transcriptionist
United States - Last active: 29 days ago - Tests: 10
Let my 20+ years of experience as a transcriptionist and legal assistant help you present your business in the best light! With Word and WordPerfect knowledge your transcripts can be done accuracy and with the highest quality possible. My background knowledge is diverse to include legal, medical, real estate, construction, oil and gas, technology and general business. If you need your letters, reports, interviews or spreadsheets to be professionally done and present your best image, let me help! All work is strictly confidential and I am HIPAA certified. My rates may be high, but your satisfaction is guaranteed!
Freelance Writer, Editor & Destination Marketing Expert
United States - Last active: 08/21/2013 - Tests: 2 - Portfolio: 1
Experienced magazine and online content writer and editor who has spent more than 15 years shamelessly promoting the people, places, organizations, events and history of the South Carolina Lowcountry and Coastal Empire of Georgia, both in print and online. Author of real estate blogs, countless feature articles, interviews, personality and business profiles, entertainment highlights, event calendars, advertorial, charts and more. Proficient in Microsoft Office products, especial Word and PowerPoint, as well as WordPress. Experience with SEO, keywords and tags. Specializing in the creation and editing of clear, concise and compelling copy.
Professional Writer and Editor, degree in English and Creative Writing
United States - Last active: 11 hours ago - Tests: 5 - Portfolio: 2
I graduated in 2010 with a Bachelor of Art in English and Creative Writing. In the years since graduating from college I have held positions as an Editor in Chief, Administrative Assistant, Chief Blogger, and various other positions wich involved a great deal of professional writing. I believe my writing ability is what sets me apart from other freelancers on the site. I am also adept at administrative tasks such as data entry, creating schedules, communicating with clients, and other organizational duties. Some of my skills include typing a clean 90 WPM, basic HTML knowledge, a strong proficiency in all Word software, professional editing, writing, administrative tasks, and having 11 years of professional experience in these fields. As you can also see from my tests I placed 1st out of 400k+ test takers on the US Spelling Test. Aside from the professional description above I am absolutely in love with writing and words. I've always been told I have a knack for writing the perfect description that people never knew they wanted. Since I was young I devoured books like The Hobbit and a lot of children's books. I decided after college I wanted to devote myself to actually doing what I went to college for. And living in a small town doesn't lend well for writing outside of your home. So I decided the best step to take was to become a freelancer and help people all over the world with their writing needs. I've written everything from stories about video games to a memorial for a loved one. I hope by reading this you can see just how much I love to write. If you're looking for someone who is truly passionate about reading, writing, and language in general, I hope you contact me and let me assist you with the perfect phrase. - Ashley
Christian writer, freelance writer, Accounting Manager, Probate Admin.
United States - Last active: 1 month ago - Tests: 3 - Portfolio: 1
Betsy C. Wise writes content that is far from commonplace, considered a word crafter providing top-notch pieces for her clients. Founder of Live The Word Ministries, Betsy touches people with her writings that directs them back to Jesus. Her website, www.LiveTheWordMinistries.org brings refreshment and encouragement to those hungry for more of God. Betsy also has a strong background in accounting and legal industries and brings excellence to every project that she tackles giving her best to each and every project.
Project Research and Portuguese Translation Specialist
United States - Last active: 4 days ago - Tests: 13 - Portfolio: 1
To obtain a position in which I am able to utilize my writing skills, may it be through translation, blogging, or editing, or related projects. I prefer to work with research and translation/transcription. I am an American who spent 5 years living immersed in the language and culture of Brazil, and have vast knowledge of all Brazilian and living abroad topics, as well as over 10 years experience with computers and internet navigation. I also have HTML knowledge. I have recently started my own project in the form of a recipe blog, and I have discovered this to be a strength of mine. I am friendly, outgoing, and can lead or work within a team. I have several years experience as management and spent a year running my own small business. I currently aid my husband in running his own small business, with much success. I am also very good at translating Portuguese to English. I am also able to understand Spanish.
Experienced - Knowledgeable . . . Writer, Editor
United States - Last active: 1 month ago
I have been in academia for over 30 years and have taught English, upper-level mathematics, chemistry, biology, and physics. I am the primary member of an animal research oversight committee at a major US research university. I have been a part of the North Carolina Association of Biomedical Research for over 15 years. I have a B.S. in Science/Math, an M.Div in theology, and an Ed.D. in Educational Administration. I have extensive experience in writing and editing. I'm not looking for a 40-hour-a-week job . . . I already have a fantastic set of classrooms where I love teaching the students. But, I am looking for selected opportunities to show you what I can do and keep myself active in the field. I've written a Pennsylvania, Junior-High history textbook (text only, not pics, etc.), one dissertation, numerous web pieces, resume editing, and numerous short pieces of a religious nature for weekly distribution to parishioners. Give me a try; I think you'll be surprised!
Paralegal/Customer Service/Legal Research/Contract Management/Manager
United States - Last active: 3 days ago - Tests: 10 - Portfolio: 10
I have been providing Legal Research and Writings, Drafting and Answering Legal Documents, Contract and Agreement Drafting and Monitoring, Quality Assurance Calls, Inbound Phone Support, Outbound Phone Support, Social Customer Support and Exit Interviews service for over 16 years. I am a Paralegal and I also have a wealth of experience using Microsoft Word, Microsoft Excel, Lexis Nexis, West Law, Google Drive and Dropbox. I'm a quick learner and I look forward to working with you in a Legal or Customer Service Rep roll. Please feel free to contact me with the projects that you need assistance with. FAST ACCURATE DRAFTING OF PERSONAL INJURY DEMAND LETTERS I specialize in the fast and accurate drafting of Personal Injury Demand Letters that effectively communicate the severity of your client's injuries and property damages. The result is a demand letter that encourages fast case settlement. This Is What You And Your Client Get For Your Money! A review of your client's medical records; A review of your client's medical bills; A review of the accident report or other documentation of the incident; Legal research re: if law applicable to your case; The drafting of the Demand Letter (Exhibits must be provided by you and your client): The Demand Letter may include (at You and Your Client's request) discussion of: The Insurance Adjusters Contact Section; The Description of Collision Section; The Initial Symptoms Section; The Initial Treatment Section; The Substantive Treatment Section; The Causation Section; The Reasonableness of Treatment Section; The Economic Damages Section; The Long Term Effects of Diagnosed Injury Section; The Non Economic Damages Section; and The Demand Section (amount of the demand as determined by you and your client) Sections Are Added or Deleted Upon You Or Your Client's Request. The Demand Letters are/will be billed at a flat rate based on the amount of your client's medical bills as follows: $150.00 for demands involving medical bills up to $20,000; $175.00 for medical bills between $20,001 and $50,000; and $200.00 for medical bills over $50,000. "COLLECT YOUR $JUDGMENT MONEY$ NOW!!!" IF JUDGMENT RECOVERY IF Judgment Recovery will use every method at our disposal to recover the full amount of the unpaid judgment plus any interest that has accrued since the judgment was issued, if you have a valid court awarded judgment of $1,000 or more. There is no up-front cost to you. There are no fees no hidden charges, only an agreement that a percentage of the judgment collected be paid as consignment. Ranked in top 25 Best Virtual Assistant for June and July 2009. Over 15 years of experience as a Paralegal, Legal Assistant, Legal Researcher. My strengths in the legal field are; attention to details, file management, research using databases such as Lexis Nexis and West Law, etc., analyzing, editing, and composing documents. And over 12 years providing services online as a Virtual Assistant. Over 16 years experience as a Private Detective and Operations Manager; Security and Loss Prevention. My strengths as a manager are in screening, recruiting, hiring, training, managing and maintaining a high level of employee production and performance while maintaining or exceeding a monthly corporate set P&L. Below please find a listing of some of the services that I provide: Legal Research and Writings Marketing Research Competitive Research Business Research General Research Prior Art Research Real Estate Research Draft and answers litigation (court) documents: Complaints Oppositions Requests Interrogatories Contracts and Agreements Independent Contractors Agreement Non-Disclosure Agreements Sales Rep Contracts Employment Agreements Distributor Agreements Confidentiality Agreements Technology Agreements Lease Agreements Warranty Deeds Grant Deeds Quit Claim Deeds MOU Divorce Documents Foreclosure litigation assistance Draft demand letters Customer Service Management Retail Merchant Credit Restoration Judgment Recovery Inside Sales/Customer Support Sales Processing Quality assurance calls Personal Assistant Document Conversion (PDF to Word) (Word to PDF) Title Act Review & Summary Just to name a few! Computer skills in Windows 5/98/2000/XP/Vista, Internet, Needles, ProLaw, Abacus, Microsoft Office, Corel 8, CRM, PDF to Word Conversion, Lexis Nexis, West Law, LexisOne, Claris Works, Word-Perfect and other software applications. PROFESSIONAL REMOTE SERVICE SPECIALIST My profile and portfolio is available for your review at: http://www.odesk.com/users/~~a4aaa16cdacc32d3
- Paralegal Services
- Legal research
- Westlaw
- Contract Drafting
- Microsoft Word
- Microsoft Excel
- Legal writing
- Wordperfect
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- Microsoft PowerPoint
- Microsoft Outlook Development
- Microsoft Access Administration
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- Windows XP Administration
- Editing
Associated with: Information Finders
2D / 3D Extrordinaire_ Retouch Artist, 3D Modeler, Animator, Rigging
United States - Last active: 11/18/2013 - Tests: 10 - Portfolio: 10
To provide high quality and direct imagery through which a story can be told. I am able to take that project to the next level through either 2 dimensional or 3 dimensional art. I am a photo retouching artist for product, although I specialize in model retouching as well. I have a thorough understanding of color theory and balance. I look forward to taking on your next project.
- Graphic design
- Adobe Photoshop
- Adobe Illustrator
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- Adobe After Effects
- Autodesk 3D Studio Max
- Autodesk Maya
- Autodesk Mudbox
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- AppleScript
- Microsoft Word
- OpenOffice
- Microsoft PowerPoint
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- Video production
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Editor/Proofreader/Web Research/Medical Biller
United States - Last active: 12/29/2012 - Tests: 6 - Portfolio: 4
I have been an Independent Contractor for 6 years. I have been successful as a team player, as well as working independently. I have a strong computer skills background with both various computer software packages as well as a standardized software medical system in both a hospital and single doctor practice setting. I have earned a certificate in Medical Office Administration, as well as have been working toward a degree in Information Technology. Through the college I have been attending, I was invited to join the Phi Theta Kappa organization due to my grades/grade point average which I was thrilled to accept and be a part of. I would like to be matched with an independent contractor position where I can utilize both my computer and organizational skills. Precision work with fast turnaround.
- Microsoft Excel
- Microsoft Word
- Email Handling
- Microsoft PowerPoint
- Medical Billing and Coding
- Internet research
- Database Administration
- database management
- Forum Moderation
- Forum Posting
- Word processing
- Wordperfect
- Order Entry
- Order processing
- Editing
- Photo Editing
- Adobe Acrobat
- Transcription
- Administrative Support
- Medical Records Research
- Document Conversion
- Document review
- Photo Manipulation
- Proofreading
- Image Editing
- PDF Conversion


text:
Tip of the Day
Sharing Photo Streams as a Web Site
The new Shared Photo Streams feature in iOS 6 is a great way of quickly and easily sharing photos directly with friends and family directly into the Photos app or into iPhoto or Aperture on OS X. For those who don't own a Mac or iOS device, however, Apple also allows you to publish your Shared Photo Stream as a public web album directly from your device. When creating a Shared Photo Stream, simply toggle on the Public Website option—you can even leave the "To" field blank if you only want to share your Photo Stream on the web. Once the Shared Photo Stream has been given a name and saved, simply tap on the blue arrow beside the Shared Photo Stream name to return to the sharing screen where you will see the URL displayed along with a Share Link button to easily allow you to send the link out to friends and family via e-mail, Messages, Facebook or Twitter. The web version of the Shared Photo Stream uses a fixed Apple template that cannot be customized, with photos presented in a tiled view in order by date added to the Shared Photo Stream with the most recent at the top. You can also toggle the Public Website option on for any of your existing Shared Photo Streams or turn it off at any time if you want to make your Shared Photo Stream private again.
Recent Tips
- Moving photos from main timeline into albums in iOS 8
- Sending locations from your desktop to your iPhone in Google Maps
- Bringing back shaped buttons in iOS 8
- Using your iPhone as a level
- Using Quick Website Search in Mobile Safari
- Updating your Apple TV Screen Saver via iCloud
- Sending to iCloud Shared Photo albums from other apps
- Find My Friends: Consolidating friends from multiple accounts
- Showing badge count for only important messages in iOS Mail
- Navigating through songs and videos at finer intervals
- Suspending or removing Apple Pay via iCloud
- Manually refreshing Passbook passes
- Previewing Link URLs in Safari
- Showing only downloaded books in iBooks
- Resetting your Home Screen Layout
- Inserting Pauses when Dialing a Phone Number
- Setting a Sleep Timer on the iPhone
- Managing Saved Passwords and Credit Cards in Safari
- Accessing Recently Closed Tabs in Safari
- Applying and removing Filters in Camera + Photos
- Quickly reading and replying to Messages
- Adjusting Siri's volume
- Naming your Fingerprints for Touch ID
- Quickly toggling the Predictive Text bar
- Showing full conversation threads in iOS Mail
- Getting haptic feedback for walking directions in Google Maps
- Filtering iMessages from unknown senders in iOS 8.3
- Going on 3D Flyover Tours in Apple Maps
- Using Thread Notifications in iOS Mail
- Displaying Emergency Contact Info in iOS 8
- See all Tips. »
Recent News
- Parrot announces North American details for new Minidrone lineup
- AppleCare+ now covers battery replacement at 80% of original capacity
- Elton John, Pharrell, Dr. Dre, Drake, St. Vincent, others to host Beats 1 shows
- Apps containing Confederate flag pulled from App Store
- Taylor Swift's '1989' album to stream on Apple Music
- Apple publishes detailed list of Siri HomeKit commands
- Apple to pay 0.2 cents per song during Apple Music free trial
- Pharrell's new single will be exclusive to Apple Music
- U.K. Apple Pay won't require a PIN, but likely limits transactions to £20 or less
- Report: Apple Music cut deals with prominent indie labels, rights groups
Recent Reviews
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Recent Articles
- CE Week 2015: IK Multimedia, Monowear's Apple Watch bands + More
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- Will removing a credit card from Safari also remove it from Apple Pay?
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- Why doesn't Traffic show up on my Today Notifications Screen?
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- Why can't I see the iPad-style landscape view on my iPhone 6 Plus?


text:
It is common knowledge in our industry that there is one undisputed leader in reporting on the topics, businesses and trends that impact all of us the most, and it is shop-eat-surf.com. Their access to those who make our industry happen is second to none, and we value not only the content of their reporting but the editorial thought on what it all means both in the present as well as the future. If we were asked to give a rating, it would be five out of five stars.- By Craig Levra, CEO, Sport Chalet
I read Shop-Eat-Surf at every opportunity, and I waited too long to sign up for the Executive Edition. It's timely, relevant and clearly "from the industry, for the industry.- By Andy Laats, President, Nixon
ISPO: Shanghai trade show runs July 2 - 4.
SIA: Debuts new "Snow Source" email and blog.
Details on Industry Insight.
Press Release:
Free Lift Ticket Offer Returns for SIA Snow Show Attendees
SIA and Colorado Ski Country USA partner for a third year in 2012
MCLEAN, Va. (Nov. 1, 2011) - What has become a tradition for Colorado Ski Country USA (CSCUSA) and SnowSports Industries America (SIA) is back again for attendees of the 2012 SIA Snow Show (Jan. 26-29).
Registered attendees get the chance to Ski/Ride the Best of Colorado with free and 2-for-1 lift ticket vouchers from 21 participating CSCUSA member resorts. Lift vouchers will be valid for five days following the Snow Show from Jan 30th through Feb 3rd at twenty-one CSCUSA member resorts.
"Colorado Ski Country USA is thrilled to partner with SIA again this year and offer free skiing and 2-for-1 lift vouchers at our member resorts. It's a fantastic way for Show attendees to explore Colorado, visit someplace they haven't been before, discover the variety of terrain we have here, and get a thorough sampling of our snowy winter goods," said Melanie Mills, President of CSCUSA.
"With all the snow we're hoping La Niña will bring this season, I know our industry will be taking advantage of their time in Colorado and these great lift ticket offers," commented David Ingemie, SIA's President.
Here's how it works - attendees register for the 2012 Snow Show and three lift ticket vouchers will accompany their Show badges. There are two separate voucher offers - Snow Show Buyers will receive three FREE lift ticket vouchers and all Snow Show Attendees will receive three 2-FOR-1 vouchers.
Registered Buyers: Three FREE lift ticket vouchers
Registered Attendees: Three 2-FOR-1 lift ticket vouchers
How to Redeem: Present voucher & Show Badge at participating ticket office
Dates Valid: January 30 - February 3
Locations: Participating Colorado Ski Country USA resorts (see below)
Arapahoe Basin
Aspen Highlands
Aspen Mountain
Copper Mountain
Crested Butte
Echo Mountain
Monarch Mountain
Purgatory (Durango)
Ski Cooper
SolVista Basin
Winter Park
Wolf Creek Ski Area
Colorado Ski Country USA (CSCUSA) is the not-for-profit trade association representing Colorado's 22 ski & snowboard resorts. CSCUSA is the global voice of the Colorado ski industry. The Association's primary functions are concentrated in marketing, public policy and public relations. Information about CSCUSA and its members can be found at www.ColoradoSki.com, on Twitter @ColoradoSkiUSA and on Facebook at www.Facebook.com/ColoradoSkiCountryUSA. This release and other press information can be found in the online press room at coloradoski.com/media-room.
SnowSports Industries America (SIA) is the national not-for-profit, North American member-owned, trade association representing suppliers and service providers of snow sports products with constituents in the retailer, rep and resort communities. Established in 1954, SIA annually produces the SIA Snow Show, the largest snow sports industry trade show, on-snow demo and networking environment globally, while delivering invaluable data/research reports including the Snow Sports Market Intelligence Report, SnowSports RetailTRAK and Executive Market Summary.
SIA helps grow the industry with consumer initiatives and websites, snowlink.com and wintertrails.org, marketing products, services and programs helping our Members operate more efficiently, bring their products to market and increase participation in snow sports. For more information, check outsnowsports.org. SnowSports Industries America, 8377-B Greensboro Drive, McLean, VA 22102-3587. Phone: 703.556.9020, Fax: 703.821.8276, Email: email@example.com. siasnowshow.snowsports.org


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Welcome to Genki
|Welcome to Genki: UK based home to niche gaming from an era when only domestic markets were considered in making a game (whilst keeping an eye over the neighbours fence for any modern day masterpieces...) Established in 2007. Happy gaming.
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Genki News
|Please note orders placed from now (until 16:00 GMT on the day itself) will be sent on Monday 06/07/15. Thanks for your attention and have a nice weekend...
Game Of The Week
|Biohazard 0 (New) (Sale)
Capcom remains the master of survival horror as it tweaks the Resident Evil engine to come up with a masterpiece of GC manipulation - not that you'll be wanting to get too close to viewing the detailed textures. And not that its only the visual splendour Capcom considered: items can now be left on t... Click for more info
Game Of The Week
|Burning Rangers (New)
Sonic Team once again underlines it's prowess and originality when given a free hand. Saving trapped souls from burning buildings is the order of the day. Unfortunately coming too late in the day to save the Saturn...... Click for more info
Recommended Game
|EX Troppers (New)
Seamless cel shaded avatars that come to life and enjoy Cybernator style chit chat as our hero charges into the screen. The independent running and targeting of enemies to shoot works well and is worthy of mention being such a crux to the success of the game. Players also take to space and Capcoms s... Click for more info
Recommended Game
|Otoko Juku Limited Edition (New)
Otoko Juku could translate as School for Hard Knocks as players get to beat each other up in the interests of manliness. Rhythm action style button presses are needed for the bonus levels as well as balancing burning pots of fire on your head and hoping it doesnt catch ablaze. The fighting is quite ... Click for more info
Recommended Game
|Sega Ages Space Harrier (New)
Flying into the screen in a 3D effect, Sega's Super Scaler techinique was quite a jaw dropper in its day. Requires fast reflexes to shoot or avoid the incoming obstacles - ideal for a quick pick up and play. Produced by Out Run, Virtua Fighter and Shenmue developer Yu Suzuki. Sadly there were no Mas... Click for more info
Recommended Game
|Shooting Love Collection (New)
Triangle Service blazes its return on the 360 which has become the spiritual successor to the Dreamcast in holding the shoot em up torch aloft and lighting up the skies. Fans of the Dreamcast titles will recall a bandana coming with the release of Trizeal and this package includes two fine releases ... Click for more info
Recommended Game
|Senran Kagura 2 Shinku Nyuu Nyu Pack (New)
Here is the formula behind the series: play as a pretty lady with some serious weaponry, such as pigtails with rotating blades. Clear a small section of the stage by taking on hordes of adversaries, often fellow ladies. Progress through the level to meet the boss. Power up and reveal (via an anime s... Click for more info
Recommended Game
|Soukyugurentai Oubushustugeki
Unique lock on system allows you to unleash untold destruction. Massive bosses. Clever sprite scaling makes for a great looking Tokyo to fly over as enemies scale in and out of range. Lovely attention to detail as shrapnel plummets to earth.... Click for more info
Recommended Game
|Gunhound EX (New)
Genki cut its teeth on the likes of Cybernator on the Super Famicom. Alas the teeth whilst longer, remain little blunt when it comes to lightening reflexes. Still the similarities are stark between the two titles and such news is cause for celebration for fans of old school 2D shooters harking for t... Click for more info
Recommended Game
|Fighting Climax (New)
A collaboration between the publishing arm of Media Works, who made the likes of Catherine and the fine reads in the Dengeki Dreamcast and Saturn magazines, and Sega to celebrate the twentieth anniversary of Dengeki. As such speed lines and sleek characters in bike jump suits meet the fine mechanics... Click for more info
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|Super Hang On 20th Anniversary Collection (New)
Brought out to celebrate two decades since the release of Super Hang On with tunes that bring back memories of sit on bike arcade machines, relentless throttle and leaning into corners. A bad CD to be listening to in any form of transport where you have control over your speed. ... Click for more info
Hardware Of The Week
|Japanese Sega Super 32X Console
Much maligned on release mainly due to the lack of support and lack of clear distinction in performance from the 16 Bit generation. Yet time has been kind to the 32X and there remains some real collectable gems and affordable arcade arranges for the system. One for the Sega aficionado.... Click for more info
Recommended Hardware
|New Super Mario Brothers U Fire Mario (New)
A lovely desktop treat for fans of Nintendos famous moustached mascot makes a Koopa feel the full force of a fireball accompanied by cheeky tune when his head is pressed. ... Click for more info
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|Japanese 3DO Console FZ 1 (no box or manual)
The 3DO dream was a very brave one allowing many manufacturers to produce hardware taking as much advantage of the multimedia aspect that was seen as the future of gaming and taking on the dominance of Sega and the Big N at the same time. And whilst the headlines screamed DOA and 3DOH!, The 3DO ha... Click for more info
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|Handle Controller
Lavishly retro with Hori's high production values, this fine controller lists the following games on the Famicom it was compatible with at the time of production: After Burner, Knight Rider, Space Harrier, Pole to Finish, Sky Destroyer, Highway Star, but others will also work. On the Mega Drive its ... Click for more info
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|Sega Saturn Micro Fibre Cloth (Segata Sanshiro) (New)
Keep your tablet or indeed Sega Saturn in spic and span condition with this fine design cloth. Segata Sanshiro became a cult hero in the fight for the Saturn's domination and he is well worthy of preserving in the Saturn hall of fame in this cloth. Can be framed for the true fanatic.... Click for more info
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|Thunderforce V Postcard Set (New)
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Cordelia Low Rise by tekweaponsWatch
©2011-2015 tekweaponsMature Content
More awesome stuff.
The one with the background is better.
Now that's a nice bottom-clevage.
nice jeans
Tek, you are amazing. You do such good work! SO Impressed.
Goddamn the color job is fantastic!!
..... "All my friends call me low rider" Heh hot work ere
Makes me wish her booty was the same size as her bust but she still looks very sexy. Fantastic job.
At one point, they were the same size... then... I don't know what happened. I guess the breasts won the arms race
Okay, this is just too sexy. The bubbly bum has me hooked. :3
This drawing is super!! How to make the background is transparent?? What software? Can work with 'Paint'??
Paint? Why use that when you can use something free AND good?
Saving the file as a .png will give you the transparent background and all that fanciness.
Saving the file as a .png will give you the transparent background and all that fanciness.
it doesn't work with paint, but most other programs have transparency features.
Whoah! o:


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Both Braham and Cambridge-Isanti were rewarded for their 20-match win volleyball seasons by earning No. 1 seeds for this week's playoff start. Braham collected one of the four No. 1 seeds in the pod set-up for the Section 5A brackets. The Bombers received a first-round bye and will host the winner...Read More
cambridge-isanti volleyball
Cambridge-Isanti, with its 20-7 record on the season, earned the No. 1 seed for the 2013 Section 7AAA volleyball playoffs. The Bluejackets will host the Oct. 25 quarterfinal match between either No. 8 St. Francis or No. 9 Princeton. With a win that night, the Bluejackets would get another home match...Read More
Cambridge-Isanti volleyball advanced its record to 15-5 overall with two victories, including a wildly exciting 20-25, 25-22, 17-25, 25-19, 15-13 rally over visiting North Branch Oct. 8. The Bluejackets answered the Vikings' win in Game 1 by jumping out to an 11-2 lead in Game 2, highlighted by a ...Read More
SPORTS ROUND-UP: Cambridge-Isanti volleyball went 3-1 to place 2nd at Saturday's annual Duluth East Invite. The Bluejackets opened with a 25-19, 25-15 win over Barnum and also beat Grand Rapids 26-24, 21-25, 15-10 in pool play. In the championship brackets, C-I rallied against Forest Lake 19-25, ...Read More
Cambridge-Isanti defeated Chisago Lakes in four games Sept. 19, taking the Mississippi 8 match 25-19, 19-25, 25-15, 25-22. The victory evened the Bluejackets' conference record at 2-2. Claire Sheehan paced the Jackets at the net with 24 kills. Also providing kills against the Wildcats were Korbyn ...Read More
Cambridge Christian volleyball stayed on a good roll this season, improving its record to 5-0 with a 25-19, 25-22, 19-25, 25-22 victory over visiting Fourth Baptist of Plymouth in a home match Sept. 17. Serving runs by Kim Walburg and Karly Wiek created breathing room in the Game 1 victory, along w...Read More
SPORTS ROUND-UP: In Mississippi 8 volleyball action Sept. 5, Cambridge-Isanti knocked off host St. Francis in four games: 25-18, 24-26, 29-27, 25-20. The Saints were the Section 7AAA runners-up last season. Claire Sheehan led at the net with 23 kills and 2 blocks. Korbyn Felde added 12 kills and 2...Read More
Braham jump-served very well– missing just three serves all night– to take down rival Rush City 25-14, 25-17, 25-12 in a key Great River match at home Sept. 3. "We were able to go on some runs because of consistencies in running our offense and making our serves," said Bomber Coach Britne En...Read More


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When this sexy blonde complained that no guy could satisfy her, her boyfriend decided to give her a treat. Instead of just one guy, the hot babe got an entire gangbang of hard cocks to satisfy her hungry pussy and mouth. In the cum swallow gangbang video, this blonde is laid out like a buffet table for the group of guys. They take turns stuffing their massive cocks into her mouth,
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Use the tools below to refine your search by only displaying reviews with a certain number of star ratings or to only show reviews from a certain time period. Eg click on '1 star' to just display the reviews we have which received a 1 star rating click or 'Within the last month' to display just reviews posted over the last month.
Written on: 27/03/2015 by DANIjabs (1 review written)
AVOID! What they first offer is not what they will pay after "reviewing" it. They don't pay when they say they will. Waiting for payments since 20/03 when I accepted the counter-offer. They said money would be in account on 3rd day, this was one week ago. AVOID! Reporting them to Trading Standards and Police (no payment = phone theft)… Read Full Review
Written on: 20/03/2015 by Bbking6433 (1 review written)
I gave them a fully functional iPhone i was told I would get my money in three working days it's been the 10th day and I still haven't been paid I have called three times and I am always been told it would be there by close of business,they lie and should be shut down forever… Read Full Review
Written on: 18/03/2015 by Whitaker275 (1 review written)
Although all the Iphones we sent were working when we purchased new iphones they advised that 1 item was of no value as it wouldnt power up. This being the case my expectation was that it would be returned.They offered no proof and would not send the phone back to us… Read Full Review
Written on: 16/03/2015 by paulwrutherford (2 reviews written)
The funds that they promised for Friday last were to pay for my disabled daughters adventure weekend which she could not attend due to lack of funds. I was assured they would be in my account my midnight Friday. It is now Monday. The CEO has failed to respond to the media, so I have asked for the phone back, but will also instigate legal proceedings and media coverage for "theft and misrepresentation". Have no dealings with this company and court papers will be submitted through the media… Read Full Review
Written on: 13/03/2015 by 300Reynolds (1 review written)
I sent my iPhone 5 with a box in perfect working condition and no marks on phone at all unlocked to any network and they offered me £116 which I was happy to accept once received they emailed me to say they will offer me £40!!!! R they joking!!!! I declined the offer and they made another offer of £60!!!! I declined again and asked that the phone be returned with the box also! I received the phone today with scrathes on the corner in a small envelope barely able to protect the phone from… Read Full Review
Written on: 11/03/2015 by danasword (1 review written)
Looked on internet to sell my S3 and this was the higher amount at £72 they were offering me phone was in good working order and just a few scratches on hand set. They sent a pre paid envelop so sent phone on the weds and they eould have got it at lastest fri 48hours delivery! 3Day bank transfer nope never reciverd payment or any emails so phone up everyday for aweek they couldnt understand why the money wasnt going in to the bank and said they would then get the money in my bank by 12midnight… Read Full Review
Written on: 08/03/2015 by Ortiziw91 (1 review written)
I have read all the reviews and I think we should just all team up and get them shut down.There are a bunch of liars ,scamming us and not paying our money .AFter numerous phone calls I got told my money would be in by end of business yesterday so I was like finally but I checked my account there was nothing,checked in the morning there's nothing.......Now tell me what's so difficult these days with just transferring money into a bank account???its been 10 working days n for the past 5 days I… Read Full Review
Written on: 05/03/2015 by Bry2015 (1 review written)
Sent an iPhone over two weeks ago and have still not received a payment despite there claim to pay out within 3 days. I have received all the confirmation emails saying payment is on the way but nothing. Have been chasing but they don't reply to emails. The last email I received stated they were having IT issues and payments were bouncing back. Looking at reviews it seems to be a long ongoing IT issues? Or more like bull. Send you goods to a proper company and avoid this one… Read Full Review
Written on: 26/02/2015 by 260McMahan (1 review written)
Was going to sell my old samsung s3 to this company, got a quote for working handset £72, i though that is a good price. When they got their hands on it told me it was scratched and would give me £20, So you can i kindly decline the offer.look on their website find your phone the choices are working, faulty or new. People their are better recycling companies out there, who give you what they say!!… Read Full Review
Written on: 16/02/2015 by KateGillespie (1 review written)
I would7ld not use them again or recommend to anyome. Websitr offers ypunone amount snf they email and try to rip people off. Money still not been paid out to me after 4days and emails I am still waiting. avoid thrm if you can… Read Full Review
Written on: 24/02/2015
PLEASE PEOPLE KEEP AWAY FROM THIS COMPANY i was going to sell my samsung S3 to them got a quote for £72, when they got my handset told me it was scratched and would give me £20. It was to years old and expects cosmetic damage. So you can imagine i decline the offer of £20.
Written on: 11/02/2015 by DanielaLeblanc1954 (1 review written)
If you had even a thought of using this company, DONT BOTHER! Awful awful awful company. Very quick to take my phones, as you would imagine. Then when it came to payment.....grind to a halt. I had all the excuses of why they hadn't paid, couldn't pay, bank hasn't processed payment, bank computers have crashed, etc etc. same day payment turned into two weeks..... Please do not use this company, trust this and other reviews you read....or accept your fate… Read Full Review
Written on: 11/02/2015 by Evie113 (1 review written)
I was offered £122 for my iPhone 5 on the basis that it was in good working condition. When iGadgets received the phone they revised their offer to £70 on the basis that it was in poor condition. I rejected that offer and asked them to return the phone to me, and I asked them on what basis they had deemed it to be in poor condition. They replied to say that dust had got between the case and screen protector, but did offer me £94. Again I rejected their offer and repeated my request that they… Read Full Review
Written on: 10/02/2015 by AndyLabrum (1 review written)
I sent an iPhone 4S in good condition and 'unlocked', boxed and with a charger and paperwork. They offered £82. Shortly after they received it I was offered £50 and told it was in poor condition. It had been screen and body protected from new. I asked for it back, they offered £58. I asked for it back, they offered £64. I asked for it back. They sent it back.. Minus the box and charger! I asked twice more for the box and charger and eventually got them back. I then used mobilecashmate.co.uk… Read Full Review
Written on: 04/02/2015 by MrsHamilton (1 review written)
Found this company through a comparison website and their offer of £79 for my 20 month old Samsung S3 although not great was ok I was only selling it because I was given a new mobile as a xmas gift. Sent the mobile in mobile box as it was in great condition, wanted to keep it that way. They emailed me with a new offer of £10 stating faulty volume button, I refused and they countered with an offer of £23 again I refused. This was a quality phone, no way was it worth only that, so refused… Read Full Review
Written on: 04/02/2015 by Hubbardrs83 (1 review written)
Initial value given was low but still enough to draw me in. Once they had the two phones that I sent to them they gave a ridiculous valuation. The only reason that I accepted the paltry amount that was offered was because I could not be bothered to to arrange be home when the phones were posted back to me. Will never use this company again and will advise anyone else not to use them either… Read Full Review
Written on: 01/02/2015 by Nicholass1947 (1 review written)
Initially things started well. Offered what they had stated online. Payment was a joke. Very, very late after many emails. They claimed to have sent it between the 16th & 26th. All lies! They tried to make out I was lying about receiving payment! Eventually received late on the 26th. DO NOT TRUST! All these people can not be wrong. Go to a trusted company. I'm one of the lucky ones who actually received my money which I was desperate for! Avoid avoid avoid!!!… Read Full Review
Written on: 29/01/2015 by klick2c (1 review written)
sent my 5 month old s4 to them which i had been offered 128 pound for and was happy with the offer.The phone had a phone cover and screen protector and worked perfect so i didnt anticipate any problems.After a week i decided to check my emails because i hadnt received my cheque.They had revised the offer to 40 pound(apparently my loudspeaker was crackly and my screen adhesive was visible ridiculous!)and said i need to reply within 72 hours but by this time it was about 90 hours since the… Read Full Review
Written on: 29/01/2015 by npotter (1 review written)
Terrible company despite numerous emails and calls and promises of payment, no payment made. They have in effect stolen a new iphone 5 c. Do not use not worth the hassle N Potter… Read Full Review
Written on: 27/01/2015 by 405Stephenson (1 review written)
Sent my wifes iPhone 4 to them which was in very good condition they offered £58.50. I gave my wife's email address but my details but forgot I gave hers instead of mine. Now this phone had a cover on the entire time and always had screen covers, checked my bank account and had a payment of £35 in my bank so I emailed asking why I had only got £35 after another 2 emails I got reply saying the phone was inspected and was graded poor, absolutely no way this phone was poor, because 72 hours… Read Full Review
Written on: 26/01/2015 by jazzoll (1 review written)
Having been offered £119 for my phone which was in perfect working order and hardly a mark on it. I sent it to them including the original box, thinking that their 100% price guarantee was genuine. I then received an email saying they were offer me £81 as my phone was in poor condition. I asked for an explanation, which I didn't receive. So I asked them to return my phone. I then got another email upping their offer to £95. I emailed them back and said unless they were prepared to… Read Full Review
As rated by our community of reviewers
" 3 months and still no payment " Read More
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" CONSISTENTLY OUTSTANDING CUSTOMER SERVICE! " Read More
" Do no use!!!! " Read More


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Please use the categories on the left column of this website to browse the RB Productions range of tools and accessories for scale models.
There will be some delays in the processing of orders placed between 5 and 19 July 2014. All delayed orders will be processed as soon as possible after 20 July. Apologies for any inconvenience caused by this delay. Thanks for your support
We look forward to meeting you at the Scale Scotland Model Show held in the Edinburgh Airport Hilton Hotel on 5 September 2015.
We are now selling Zoukei Mura products. For more information please click here: http://www.radubstore.com/index.php?main_page=index&cPath=127_128


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Exploring new drug treatment for addictions
South Dakota State University researchers have demonstrated for the first time that a plant-derived compound used to treat nicotine addiction also has significant effects against alcohol addiction.
South Dakota State University researchers have demonstrated for the first time that a plant-derived compound used to treat nicotine addiction also has significant effects against alcohol addiction.
(PhysOrg.com) -- Heavy rains have led to a warm-up in South Dakota, and these conditions have dairy producers concerned because of the potential threat mycotoxins pose to their cows. South Dakota Cooperative Extension Dairy ...
(PhysOrg.com) -- Continuing autumn rains are producing conditions that can increase lameness and mastitis in dairy herds in South Dakota.
No matter where you get your eggs, a few basic food safety steps can keep you, your family, or the customers at your business safe. That's according to South Dakota Cooperative Extension Food Safety Specialist Joan Hegerfeld-Baker, ...
(PhysOrg.com) -- South Dakota State University livestock research is trying to determine whether the genes cattle inherit help determine the way they respond to vaccinations.
(PhysOrg.com) -- South Dakota's mosquito population is back in force this summer.
(PhysOrg.com) -- A South Dakota State University researcher and his colleagues elsewhere have discovered a previously unreported mitochondrial protein that interacts with a protein known to play a role in Alzheimer's disease.
(PhysOrg.com) -- Several western South Dakota counties have reported grasshopper hatchings and producers in these areas are reminded to start scouting for these pests.
(PhysOrg.com) -- Separating calves from older animals is effective to reduce disease exposure to unweaned calves.
(PhysOrg.com) -- The alfalfa silage harvests happening this month can shape the success of an entire year's milk production. That's according to South Dakota Cooperative Extension Dairy Specialist Alvaro Garcia, who said ...
(PhysOrg.com) -- Winter wheat producers should carefully monitor their crops over the next week to assess possible damage from late frosts.
(PhysOrg.com) -- A South Dakota State University scientist is exploring ways to re-use enzymes in processes such as making cellulosic ethanol.
(PhysOrg.com) -- South Dakota State University research shows a traditional Asian flatbread called chapathi, or chapati, gets a big boost in protein and fiber when fortified with food-grade distillers grains.
(PhysOrg.com) -- South Dakota State University mathematicians are helping doctors of the Mayo Clinic build mathematical models to fine-tune an innovative strategy to treat cancer.
(PhysOrg.com) -- Experts from South Dakota State University and the nearby North Central Agricultural Research Laboratory watched helplessly as a colony of rare, captive lady beetles was lost in 2008, then ...


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... More
1968 Lincoln Continental Coupe Very rare car and extremely rare black on black. with 77k original miles and all original car including paint and... More
For sale in our St. Louis showroom is a gorgeous 1979 Lincoln Mark V. This Mark V has only traveled 22403 miles in its... More
For sale in our Detroit showroom is a stylish and iconic 1969 Lincoln Continental. In the 60s the Lincoln was completely redesigned and shortened... More
For sale in our Detroit showroom is a stylish and classy 1977 Lincoln Mark V. In the 70s the Lincoln Mark series was the... More
GR Auto Gallery is pleased to present this 2001 Lincoln Town Car Cartier Edition Sedan for purchase. Lincoln has successfully been Ford's brand of... More
1963 Lincoln Continental Convertible, Incredible ORIGINAL car from renowned Lincoln Collector! Exterior Color: White Interior: Black Leather Mileage:49,000345 I acquired this absolutely magnificent original Lincoln 4/door Continental convertible... More
1966 Lincoln Continental Convertible--"suicide doors"(opera doors for the purists)-- CLEAN TITLE--$25000--Great car with a solid history in New England. Russet metallic exterior with a... More
1967 Lincoln Continental 4 Door Convertible for sale by Private Owner. The car is in show condition with 45,100 original miles. The car was... More
1977 Lincoln Town Coupe project car. Many NOS parts and restorable spares. Gold exterior, Gold leather interior, car originally had white landau top. Engine... More
Offered for Sale by the California Automobile Museum 1970 Lincoln Continental Sedan We Offer Financing Exterior Very light green (mint) paint appears to be original and is in... More
This magnificent car was displayed at the 2002 Carlisle All-Fords Invitational and the AACA Museum "Tail Fins" exhibit in 2008. Has the 300hp 368ci... More
This rare body style has just 30,359 original miles. The engine is the 302ci Electronic Fuel Injected V8 with an automatic transmission with overdrive.... More
A True Survivor with 26,406 Original Miles!!1 of 3,000 Mark IIs built for 56 & 57Fully Optioned with Factory Air ConditioningRobbins Egg Blue with... More
1946 Lincoln Club Coupe, burgandy with tan interior like new condition, V-12 power, new windsheld, rebuilt power windows, excellent chrome-grill & bumpers, Borg Warner... More


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