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There are over 100 recognized species of the genus Mycoplasma, one of several genera within the bacterial class Mollicutes. As a group, Mollicutes have small genomes (0.58 - 1.38 megabase-pairs), lack a cell wall, and have a low GC-content (18-40 mol%). Mollicutes are parasites or commensals of humans, other animals (including insects), and plants; the genus Mycoplasma is by definition restricted to vertebrate hosts. Cholesterol is required for the growth of species of the genus Mycoplasma as well as certain other genera of mollicutes. Their optimum growth temperature is often the temperature of their host if warmbodied (e. g. 37° C in humans) or ambient temperature if the host is unable to regulate its own internal temperature. Analysis of 16S ribosomal RNA sequences as well as gene content strongly suggest that the mollicutes, including the mycoplasmas, are closely related to either the Lactobacillus or the Clostridium branch of the phylogenetic tree (Firmicutes sensu stricto).
The bacteria of the genus Mycoplasma (trivial name: mycoplasmas) and their close relatives are largely characterized by lack of a cell wall. Despite this, the shapes of these cells often conform to one of several possibilities with varying degrees of intricacy. For example, the members of the genus Spiroplasma assume an elongated helical shape without the aid of a rigid structural cell envelope. These cell shapes presumably contribute to the ability of mycoplasmas to thrive in their respective environments. M. pneumoniae cells possess an extension, the so-called 'tip-structure', protruding from the coccoid cell body. This structure is involved in adhesion to host cells, in movement along solid surfaces (gliding motility), and in cell division. M. pneumoniae cells are of small size and pleomorphic, but with a rough shape in longitudinal cross-section resembling that of a round-bottomed flask.
Mycoplasmas are unusual among bacteria in that most require sterols for the stability of their cytoplasmic membrane. Sterols are acquired from the environment, usually as cholesterol from the animal host. Mycoplasmas also generally possess a relatively small genome of 0.58-1.38 megabases, which results in drastically reduced biosynthetic capabilities and explains their dependence on a host. Additionally they use an alternate genetic code where the codon UGA is encoding for the amino acid tryptophan instead of the usual opal stop codon.
In 1896 Nocard and Roux reported the cultivation of the causative agent of contagious bovine pleuropneumonia (CBPP), which was at that time a grave and widespread disease in cattle herds. Today the disease is still endemic in Africa and Southern Europe. The disease is caused by M. mycoides subsp. mycoides SC (small-colony type), and the work of Nocard and Roux represented the first isolation of a mycoplasma species. Cultivation was, and still is difficult because of the complex growth requirements. These researchers succeeded by inoculating a semi-permeable pouch of sterile medium with pulmonary fluid from an infected animal and depositing this pouch intraperitoneally into a live rabbit. After fifteen to twenty days, the fluid inside of the recovered pouch was opaque, indicating the growth of a microorganism. Opacity of the fluid was not seen in the control. This turbid broth could then be used to inoculate a second and third round and subsequently introduced into a healthy animal, causing disease. However, this did not work if the material was heated, indicating a biological agent at work. Uninoculated media in the pouch, after removal from the rabbit, could be used to grow the organism in vitro, demonstrating the possibility of cell-free cultivation and ruling out viral causes, although this was not fully appreciated at the time (Nocard and Roux, 1890). The name Mycoplasma, from the Greek mykes (fungus) and plasma (formed), was proposed in the 1950s, replacing the term pleuropneumonia-like organisms (PPLO) referring to organisms similar to the causative agent of CBPP. It was later found that the fungus-like growth pattern of M. mycoides is unique to that species.
This confusion about mycoplasmas and virus would surface again 50 years later when Eaton and colleagues cultured the causative agent of human primary atypical pneumonia (PAP) or walking pneumonia. This agent could be grown in chicken embryos and passed through a filter that excluded normal bacteria. However, it could not be observed by high magnification light microscopy, and it caused a pneumonia that could not be treated with the antimicrobials sulphonamides and penicillin.. Eaton did consider the possibility that the disease was caused by a mycoplasma, but the agent did not grow on the standard PPLO media of the time. These observations led to the conclusion that the causative agent of PAP is a virus. Researchers at that time showed that the cultured agent could induce disease in experimentally infected cotton rats and hamsters. In spite of controversy whether the researchers had truly isolated the causative agent of PAP (based largely on the unusual immunological response of patients with PAP), in retrospect their evidence along with that of colleagues and competitors appears to have been quite conclusive. In the early 1960s, there were reports linking Eaton's Agent to the PPLOs or mycoplasmas, well known then as parasites of cattle and rodents, due to sensitivity to antimicrobial compounds (i. e. organic gold salt). The ability to grow Eaton's Agent, now known as Mycoplasma pneumoniae, in cell free media allowed an explosion of research into what had overnight become the most medically important mycoplasma and what was to become the most studied mycoplasma.
Recent advances in molecular biology and genomics have brought the genetically simple mycoplasmas, particularly M. pneumoniae and its close relative M. genitalium, to a larger audience. The second published complete bacterial genome sequence was that of M. genitalium, which has one of the smallest genomes of free-living organisms. The M. pneumoniae genome sequence was published soon afterwards and was the first genome sequence determined by primer walking of a cosmid library instead of the whole-genome shotgun method. Mycoplasma genomics and proteomics continue in efforts to understand the so-called minimal cell, catalog the entire protein content of a cell, and generally continue to take advantage of the small genome of these organisms to understand broad biological concepts.
Scientists have also been exploring an association between mycoplasma and cancer. Despite a number of interesting studies, this cancer bacteria association hasn't been clearly established, and has yet to be fully elucidated.
The medical and agricultural importance of members of the genus Mycoplasma and related genera has led to the extensive cataloging of many of these organisms by culture, serology, and small subunit rRNA gene and whole genome sequencing. A recent focus in the sub-discipline of molecular phylogenetics has both clarified and confused certain aspects of the organization of the class Mollicutes, and while a truce of sorts has been reached, the area is still somewhat of a moving target.
The name mollicutes is derived from the Latin mollis (soft) and cutes (skin), and all of these bacteria do lack a cell wall and the genetic capability to synthesize peptidoglycan. While the trivial name 'mycoplasmas' has commonly denoted all members of this class, this usage is somewhat imprecise and will not be used as such here. Despite the lack of a cell wall, Mycoplasma and relatives have been classified in the phylum Firmicutes consisting of low G+C Gram-positive bacteria such as Clostridium, Lactobacillus, and Streptococcus based on 16S rRNA gene analysis. The cultured members of Mollicutes are currently arranged into four orders: Acholeplasmatales, Anaeroplasmatales, Entomoplasmatales, and Mycoplasmatales. The order Mycoplasmatales contains a single family, Mycoplasmataceae, which contains two genera: Mycoplasma and Ureaplasma. Historically, the description of a bacterium lacking a cell wall was sufficient to classify it to the genus Mycoplasma and as such it is the oldest and largest genus of the class with about half of the class' species (107 validly described) each usually limited to a specific host and with many hosts harboring more than one species, some pathogenic and some commensal. In later studies, many of these species were found to be phylogenetically distributed among at least three separate orders.
A limiting criterion for inclusion within the genus Mycoplasma is that the organism have a vertebrate host. In fact, the type species, M. mycoides, along with other significant mycoplasma species like M. capricolum, is evolutionarily more closely related to the genus Spiroplasma in the order Entomoplasmatales than to the other members of the Mycoplasma genus. This and other discrepancies will likely remain unresolved because of the extreme confusion that change could engender among the medical and agricultural communities. The remaining species in the genus Mycoplasma are divided into two non-taxonomic groups, hominis and pneumoniae, based on 16S rRNA gene sequences. The hominis group contains the phylogenetic clusters of M. bovis, M. pulmonis, and M. hominis, among others. The pneumoniae group contains the clusters of M. muris, M. fastidiosum, U. urealyticum, the currently unculturable haemotrophic mollicutes, informally referred to as haemoplasmas (recently transferred from the genera Haemobartonella and Eperythrozoon), and the M. pneumoniae cluster. This cluster contains the species (and the usual or likely host) M. alvi (bovine), M. amphoriforme (human), M. gallisepticum (avian), M. genitalium (human), M. imitans (avian), M. pirum (uncertain/human), M. testudinis (tortoises), and M. pneumoniae (human). Most if not all of these species share some otherwise unique characteristics including an attachment organelle, homologs of the M. pneumoniae cytadherence-accessory proteins, and specialized modifications of the cell-division apparatus.
A detailed analysis of the 16S rRNA genes from the order Mollicutes has given rise to a view of the evolution of these bacteria that includes an estimate of the time-scale for the emergence of some groups or features. This analysis suggests that about 600 million years ago (MYA), late in the Proterozoic era, Mollicutes branched away from the low G+C Gram-positive ancestor of the streptococci, losing their cell wall. At this time on Earth, molecular oxygen was present in the atmosphere at 1%, and the fossil record shows that multicellular marine animals had recently spread in the Cambrian explosion. One hundred million years later the requirement for sterols in the cytoplasmic membrane evolved along with the change to the alternate genetic code. Also, the ancestor of the genera Spiroplasma and Entomoplasma (primarily plant and insect pathogens) and Mycoplasma emerged at this time and would itself diverge into the Spiroplasma-Entomoplasma and Mycoplasma lineages approximately 100 million years after that. This diversity coincided with the origin of land plants 500 MYA. It appears that the calculated rate of evolution for the Mycoplasma group increased several fold about 190 MYA, soon after the appearance of vertebrates, while the Spiroplasma-Entomoplasma ancestor continued to evolve at the previously shared slower rate until about 100 MYA, when angiosperms and their associated pollinating insects appeared. Then the evolution rate of these bacteria appears to have also increased significantly. This is an attractive hypothesis, but while it tracks the emergence of several of the unusual characteristics of Mycoplasma and related organisms, it does not address the selective pressures driving their evolution, except perhaps the widespread close association of a parasite with a specific host. The advantages of a reduced genome, cell wall-less structure, and alternate genetic code remain murky.
Mycoplasma species are often found in research laboratories as contaminants in cell culture. Mycoplasmal cell culture contamination occurs due to contamination from individuals or contaminated cell culture medium ingredients. Mycoplasma cells are physically small – less than 1 µm – and they are therefore difficult to detect with a conventional microscope. Mycoplasmas may induce cellular changes, including chromosome aberrations, changes in metabolism and cell growth. Severe Mycoplasma infections may destroy a cell line. Detection techniques include PCR, plating on sensitive agar and staining with a DNA stain including DAPI or Hoechst.
cs: Mycoplasma de: Mykoplasmen et: Mükoplasma es: Mycoplasma eu: Mycoplasma fr: Mycoplasma it: Mycoplasma he: Mycoplasma nl: Mycoplasma no: Mykoplasma nn: Mykoplasma pt: Micoplasma ru: Микоплазмы sv: Mykoplasma wa: Micoplasse


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Aug 22, 2013Goal Zero Guide 10 Plus Adventure Kit Review
With an ever evolving world where mobile devices are being designed to go everywhere, the worst thing to see is a red blinking light and your phone or device saying "Connect Charge". When you're out in the middle of nowhere enjoying the mountains, desert, beach…there isn't a convenient outlet for you to plug your device into.Read More
Aug 9, 2013Cast Iron Skillet Biscuit and Egg Boats Recipe
Biscuit and Egg Boats Cooked in a Skillet in the Italia Artisan Pizza Oven.
By: Gary HouseRead More
Jul 16, 2013How to Make Bacon Wrapped BBQ Ribs - Video
BBQ. Bacon. Ribs. Put them together and you have a recipe for greatness!
That's what Gary House has done. He turned his BBQ Grill Box by Camp Chef into a smoker grill and shows you how to do it in this video... Oh yeah, he also will show you how to make these amazing ribs!
Jun 20, 2013How to Make BBQ Bacon Wrapped Ribs Video
The Fourth of July is just around the corner and we've been let in on a little secret of a great BBQ rib recipe. BBQ Bacon Wrapped Ribs. Celebrate Indepence Day with this delicious rib recipe and impress your guests. Whether seasoned in the art of rib making or just starting out, Gary House of Cooking-Outdoors walks through this recipe with tips and tricks.Read More
Jun 20, 2013How to Make BBQ Bacon Wrapped Ribs
Let the drooling begin! Then give these a try. Your tastebuds (and anyone else who is lucky enough to dig in) will thank you.
When we, here at Outdoor Cooking, heard what Gary House over at Cooking-Outdoors was fixing, we were pretty excited. BBQ Bacon Wrapped Ribs sounded amazing. Then we saw pictures and the video of these things... It was cruel and unusual punishment t Read More
Jun 12, 2013Homemade BBQ Sauce
By: Gary House
How could it be possible to improve on the pride you feel of placing a perfectly grilled meal on your table?
Making your own BBQ sauce!Read More

subset: commoncrawl


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Rusty Jack Klaus is the Director of Klaus Holdings, Inc. .
Klaus Holdings, Inc. filed as a Domestic For-Profit Corporation in the State of Texas filed on Wednesday, September 9, 2009. This corporation is approximately six years old according to documents filed with Texas Secretary of State.
Rusty Jack Klaus is connected to other officers through these corporate roles. One of the top connections is Carla R. Klaus .
Key roles for Rusty Jack Klaus
Klaus Holdings, Inc.
Director, President


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Kampala — Uganda's Catholic bishops reaffirmed their opposition to homosexuality, but reserved judgment on a recently ratified bill imposing harsh punishment for homosexual acts in the East African nation.
"Our reaction from the Church is very clear, we don't support homosexuality," Msgr. John Baptist Kauta, secretary-general of the Uganda Episcopal Conference, told Catholic News Service (CNS) on February 26.
He said that when the anti-gay bill was first discussed, the country's bishops had been against the harsh penalties it involved for homosexual acts, including the death penalty.
"The bishops were not in favor of that," he said. "We were for compassion, and we believe (homosexuals) can change."
He said Uganda's bishops were in a retreat and would not be available to comment on the new law until early March.
"We normally don't want to overreact," he said.
Uganda's anti-gay bill was signed into law on February 24 by Ugandan President Yoweri Museveni.
The bill originally proposed the death penalty for "aggravated homosexuality," but first-time offenders will now face life in jail, instead of an originally proposed 14-year prison term.
Western donor countries and international rights groups have termed the new law an abuse of human rights and are asking for its repeal.
"The United States is deeply disappointed in the enactment of the Anti-Homosexuality Bill in Uganda," U.S. Secretary of State John Kerry said in a statement.


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International 001.562.252.3789
Try Our Other App!
Now you can send your Zendesk contacts the professional email campaigns you create in Benchmark Email! This integration imports your Zendesk contacts into a Benchmark list of your choosing. It's fast, it's easy and it maximizes your email marketing opportunities. Available free with every Benchmark account.


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Error Message: 1635 This Patch Package Could Not Be Opened
Article ID: 812490 - View products that this article applies to.
On This Page
When you try to remove Plus! for Windows XP, you may receive an error message that is similar to the following:
1635 This patch package could not be opened. Verify that the patch package exists and that you can access it, or contact the application vendor to verify that this is a valid Windows Installer patch package.
This behavior may occur when the following registry key is damaged:
To resolve this issue, remove the damaged registry key, remove and reinstall Plus! for Windows XP, and then remove and reinstall Plus! for Windows XP again.
Important This section, method, or task contains steps that tell you how to modify the registry. However, serious problems might occur if you modify the registry incorrectly. Therefore, make sure that you follow these steps carefully. For added protection, back up the registry before you modify it. Then, you can restore the registry if a problem occurs. For more information about how to back up and restore the registry, click the following article number to view the article in the Microsoft Knowledge Base:
(http://support.microsoft.com/kb/322756/ )How to back up and restore the registry in Windows
Remove the Damaged Registry Key
Remove Plus! for Windows XP
Reinstall Plus! for Windows XP
Article ID: 812490 - Last Review: September 23, 2011 - Revision: 2.0


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Tech Connect provides options for every price point. Getting started is easy. Just select "Add My Listing" below. Create a company profile and choose among the several listing packages described below and/or the a la carte options to enhance your listing.
Each Tech Connect package includes a FREE listing in the RE Magazine Buyer's Guide—the most retained issue of the year. Over 22,000 qualified subscribers receive this issue and return to it for inspiration throughout the year. Make your presence count with a premium or exclusive Buyer's Guide listing upgrade and/or display advertising.


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A sound one makes when dying.
Red alert soldier when nuked - "HAT!"
dari bobba Senin, 08 Mei 2006
A hat can be used as another term for "ass" or "butt"
Yo that dawg has a nice hat.......I want to superman that shit!
dari Buckweed Jum'at, 13 Mei 2005
You wear it on your head....Dipshit.
Example 1. "Look, I'm wear my hat, on my head....Faggot."
Example 2. "FREE HAT, FREE HAT, FREE HAT"
Example 2. "FREE HAT, FREE HAT, FREE HAT"
dari Matt Rabu, 03 November 2004
1. Jimmy hat.
2. The foreskin of an uncircumcised male.
2. The foreskin of an uncircumcised male.
I put on a hat before I had sex with her. Fortunately, she didn't mind that my gentleman wears his hat indoors.
dari Brown Sugar Selasa, 18 Maret 2003
In ultimate disc but possibly in other sports, a tournament or entire league session in which teams are made at random--i.e. chosen from hat.
"Are you playing in the hat tournament this weekend?" "No, but im playing in the fall hat league."
dari caliphate Selasa, 29 Juli 2008
The exchangable lid/bowl for the beast. Different size hats hold different amounts of weed.
"Man, we got a lot of people hitting the beast. Throw on the 'party hat' will ya."
dari hollistercoiner Jum'at, 06 Februari 2004
Hats stands for "Huge Ass Tit's". To be used in a situation where only an inner party is familuar with the expression to point out a woman with large breast.
"Damn! look at those hats in front of us!"
dari Aaron "-...BBG...-" Minggu, 10 September 2006


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Today's gem was from a reader complaining about comments in a previous edition. 'Not all of us have access to resources to fix such things as grammar and punctuation if our educations weren't as exceptional as [Name's].' The writer goes on to add up the costs of critique and copy-editing, postage and ink, and says 'Punctuation and grammar have never been my strongest point. ... My story has just as much right to be read as best sellers. So what can a self-published author do?'
I have great sympathy with those who feel that they have trouble with reading and writing, and especially with dyslexics, some of whom are brilliant story-tellers. They have very specific problems, which can scarcely be overcome by any means other than by paying a copy editor. Yes, it puts them at a disadvantage, but most of them are philosophical about it. But what of other writers who have no diagnosed disability? Is it enough to blame a poor education?
I would have loved to be able to play tennis. It wasn't that I was never fit enough: my Saturday job as a teenager was teaching in a dancing school. But no one ever bothered to teach me hand-eye co-ordination, so I spent five years at secondary school swiping wildly with a tennis racket and being tacfully ignored, along with my fellow sports failures, by the PE teacher.
I didn't attempt to take up tennis professionally. To do so I would have had to embark on a serious course of remedial lessons in technique, and resigned myself to many years of practice, with no assurance of success at the end.
Yes, it may be that some writers know that their education failed to prepare them for their chosen career. But in these days of opportunities for mature students, literacy classes and the availability of the internet, it's not beyond anyone to find their own remedy. Lynn Price writes on the Behler Blog:
You can have a great story idea, but if you write like you barely made it out of 8th grade, then no reputable editor will take pity on you and offer you a contract. They'll kick you to the curb. Quickly.Harsh words. But they come from a professional, and she's worth listening to.
Being an expert in your craft should take precedence over your desire to be published. Sadly, I see the opposite in large quantities.
Stamp this on your forehead: If you haven't learned how to write, then you haven't planned for success.
Sorry to sound so crabbit. It's the cough.


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We are located at 2 Geylang Lor 15 . Opened daily from 5.30pm till 4am
Add us as a friend on facebook !
That Nasi Lemak Moment
Bali Nasi Lemak was established in Geylang since 1985. Being a casual and simple Nasi Lemak specialty store , it offers a variety of dishes at value prices.
We also provide lunch box sets and casual buffet catering services for all occasions.
Our Specialties
Bali signature kecap manis chicken (Famously known as "black" chicken)
Crispy golden chicken
Sambal petai
Long beans with sambal belacan
Curry chicken


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Saint-Nabord, France Hotels
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"Do not expect justice where might is right." ~ Plato
Original article "If Holder's comments Monday signal any pivot in the Obama administration's approach to Snowden, the Justice Department is so far not publicly indicating it."
Original article "Iceland is on track to become the first European country to surpass its pre-crisis peak of economic output - essentially proving to the U.S. that bailing out 'too big to fail' banks wasn't the way to go."
Original article "I was a cop who went drug dealer. That's what it is, says Jonathan Treviño, a former police narcotics squad leader who is serving 17 years in a federal penitentiary."
Original article "And despite the fact that no arrests have been made, the state has seized one home, two vehicles, $15,000 in cash, and a bevy of personal property from those suspected of heading up the alleged prostitution ring."
Original article "The officers were unable to articulate a reasonable suspicion that King was involved in a crime, but he was illegally arrested anyways for contempt of cop and pushing his luck."
Original article "As the Marshall Project reports, many citizens are illegally arrested for cursing at cop, when in fact, their speech is protected."
Original article "Two weeks ago, the string of mysterious deaths began when Dr. Jeff Bradstreet MD was found in a river with a gunshot wound to his chest. The police claim that the gunshot wound was self-inflicted and that the death was a suicide, however, Bradstreet's family suspects foul play."
Original article "Enigma's technique—what cryptographers call secure multiparty computation—works by mimicking a few of the features of bitcoin's decentralized network architecture"
Original article "Mulford found evidence that the scalping letter was reprinted a few times in London, and many more in the new United States. Thirty-five American newspapers republished Franklin's handiwork as truth before 1854, when the Trenton, New Jersey, State Gazette outed it as a hoax."
Original article "Asked what Americans were celebrating on the 4th of July, another woman said that Independence Day was to recognize the day that we overtook the south….it's our independence….from the south"
Original article "Farrington began studying the history of high school and said she soon realized that the first high schools were designed on purpose to weed out those who couldn't cut it."
Original article Part of the solution was to establish concrete arrows, painted a brilliant yellow, measuring up to 70 feet long, and were visible from a distance of 10 miles up.
Original article "Certainly, the vast majority of comet scientists would agree that comet 67P's surface features are much more easily explained by non-biological mechanisms."
Recommended Videos: Game of Thrones, Fresh, Tiny: A Story About Living Small, Happy, Once in a Lifetime, Revolution, Hating Breitbart, Rise & Shine: The Jay Demerit Story, Dark Legacy, Shelter Me, American Guns, Moonshiners, Longmire, Flying Wild Alaska Season 2, Sons of Anarchy, Gold Rush, 180 Degrees South, House of Cards, Speaking Freely: Chalmers Johnson, American Violet, The Lottery, The Devil Came on Horseback, Farmageddon, Dreams From My Real Father, End of the Road: How Money Became Worthless, Thomas Jefferson, Ground War; see recommended movies
Books: The Great Deformation: The Corruption of Capitalism in America
Music: "Bells" by Smoke Fairies
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Feeling Unbalanced? Make a DIY "Wheel of Life"
Last night, something really strange happened. So strange that I'm certain I won't be able to explain it very well in this post.
But I'll try in a moment.
First, be sure to come out to your local Michaels Arts & Craft store this Sunday, February 16 from 1-4 p.m. for the Michaels and Hometalk.com Pinterest Party! (Hometalk.com is the largest social network for home and garden enthusiasts).
The project you see below, along with several others, will be available for you to make. Just buy your materials and let's get craftin'! I'll be at the Rockville, Maryland Michael's store being the blogger-represent, helping customers with their projects! So if you want to get your craftin' on, check this list for all the Michaels that will have bloggers there. But remember, Michaels stores nationwide will be hosting this event!
(And if you follow Michael's on Hometalk, you are entered to win a $100 Michaels gift card. Five winners will be announced on Hometalk's Facebook page on Feb 17, after the party). Hope to see you there!
Now on to my project I created for the Pinterest Party–
I created this project only to find out this morning that it resembles something Buddhists have been drawing on Buddhists temples for thousands of years.
Yeah, I know…….Weird, right??
Let me explain–
Lately I've been suffering from a condition called "my-life-is-not-balanced–crap-I-need-balanceeee."
So when Hometalk.com, the largest online community for home and garden enthusiasts (are you a member?) invited me as a host for Michael's arts and crafts "Pinterest Party" this Sunday, February 16 at the Michael's in Rockville, Maryland, I wanted to use the materials list we were provided to make something that was unique and personal, and most importantly, something that spoke to what I've been struggling with lately.
I chose the stretched burlap canvas……
…and 8 wooden banner triangles….
…and created this– what I now realize is a Wheel of Life, according to Buddhists.
The Resemblance is Uncanny……
What I didn't realize when I finished this project last night is that I unknowingly channeled this Buddhist image (known as bhavacakra, which means "wheel of life"), which has been found on outside walls on Tibetan Buddhist temples.
What the—?????!!!
Where Did THAT Inspiration Come From?!
I couldn't tell ya. I have no idea.
I've never even seen this Buddhist Wheel of Life before. Never even heard the term. But down to the finest detail of a "CIRCLE" in the middle, our images are almost identical.
What's even freakier is that in the pic above, some crazy guy is holding his wheel of life, just as I am below.
And–no lie– my husband snapped this picture this morning. So many resemblances.
Only I don't have fangs. 😉
But seriously…how strange is that?! It makes me wonder if our creativity is really just our inner, authentic spirit speaking to us.
Well, I'm listening loud and clear now.
So How Do You Make Your Own DIY Wheel of Life?
Okay, enough squealing over this amazing coincidence. (WATCH THE STEP-BY-STEP VIDEO HERE)
What I like about this project is that every day…..every week….every month….you can erase and change your goals, making sure that each of the slices of your life get enough love and nurturing. It's so important.
Materials Needed:
*2 stretch burlap canvas board from Michael's (about $10)
* wooden banners from Michael's ($1.00 each)
*Cardstock in rainbow colors
*Paper cutter or scissors
*Hot glue
*Black sharpie marker
*Chalkboard paint (if banners are not already chalkboard)
STEP 1: Get Geometrical!
Lay out all the banner pieces on the stretched burlap to form this shape. These "slices" will represent one important area of your life for which you want to create goals and balance.
Grab some cardstock and get make Roy G. Biv (red-orange-yellow-green-blue-indigo-violet, for all y'all folks that don't know). Use scissors or a paper cutter to cut slips of cardstock to fit on top of the banner. You'll use these as the labels, and to cover the holes.
STEP 3: Chalk, Chalk, Baby
Okay, so I made a fatal error (gasp!!). I forgot to prime the before spraying on the chalkboard paint. Because of that, I'll probably not get as durable a finish. But you know what?? For only a dollar each, who cares?
STEP 4: Prime Time
Don't forget to "prime" your chalkboard banners. Run chalk over them like this to "set" get the chalkboard surface ready for writing.
STEP 5: Color Pie
Use Mod Podge to glue each of the colors to the top of the chalkboard banner. You should be left with this:
(P.S. This is why I love this project– all the colors of the rainbow representing harmony and balance. Woo-sahhhhhh……. And the burlap lends to the metaphor that life is "rough." Get it?? )
STEP 6: Pass Me the Glue Gun and Marker!
Now glue each of the slices down in the pattern you designated. Use a fat marker to label your slices of life :). You can stencil the names on, to make it look more neat.
STEP 7: The Heart of the Matter
Add a circle in the middle. Label it whatever you like. I like "Balance" as a reminder. Use hot glue to secure it.
STEP 8: Get Balanced!
Now you've got a cute way to select goals for each of the important areas of your life. When you're done with one goal, erase it, and pick a new one!
The most important lesson here is to be mindful of all parts of your life. It's so easy to get consumed by one area. For me, it's blogging. I looooovvvveee what I do. But as a result, I ignore my poor husband in the evenings. And my health suffers from lack of sleep or exercise from hours and hours of sitting in front of the computer. Hopefully after this project, I'll find my perfect balance.
Do you ever feel off balance? What do you do to get your life back in order?
P.S. Be sure to follow Michael's on Hometalk to enter to win a $100 Michaels gift card. Five winners will be announced on Hometalk's FB page on Feb 17, after the party.


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'Best-kept secret'Karen Yost, the national president of American Agri-Women, spoke at the 30th annual Harvest of Knowledge Agri-Women's Conference Oct. 26 in Grand Forks, N.D.
By: Jonathan Knutson, Agweek
Karen Yost likes to say that she's part of "the nation's best-kept secret."
"Even though we've been around since 1974, people hear our name and say, 'What's that?' But when they understand what we are, they're usually anxious to get involved," she says.
Yost is national president of American Agri-Women, a coalition of 50 state and commodity affiliate organizations, as well as individual women, across the country. Her group represents about 50,000 women.
Yost, of Billings, Mont., spoke at the 30th annual Harvest of Knowledge Agri-Women's Conference Oct. 26 in Grand Forks, N.D. About 150 women attended the event, sponsored by North Dakota Agri-Women and Minnesota Agri-Women. Convention topics included opportunities in agricultural exports and activism in ag.
Agweek visited with Yost before her presentation.
American Agri-Women, the members of which include the U.S. Rice Federation and the Women's Mining Coalition, works on legislative and regulatory issues at the local, state and national levels.
"A lot of our natural resource regulations are coming from people who've been educated by 40 years of Walt Disney movies. It's not really reality. In the real world, the wolf eats the rabbit. They don't play together," Yost says.
Currently, American Agri-Women's most important goal is approval of a new farm bill, she says.
Educating its own members is another priority for the organization, which is why conferences such as the one in Grand Forks are important, Yost says.
"If you're in agriculture, you want to stay up to date with the newest technology," she emphasizes. "You want to be able to know what's going on with regulations. You just need to attend these kinds of conference to stay abreast of changes in your industry."
American Agri-Women also works to educate students and consumers.
Diverse role in ag
Yost says women have always been involved with agriculture, but that the involvement sometimes wasn't acknowledged properly.
As recently as the 1970s, "Women couldn't even sign papers with the USDA. They weren't considered a legal entity. They couldn't even say, 'Yes, I want to sign with my husband' to get a loan," she says.
Today, women play an increasingly diverse role in agriculture. Farm children, both male and female, are getting agricultural degrees and working in agribusiness, she says.
"You can take the girl off the farm, but you can't take the farm out of the girl," Yost says, adding that women increasingly own and operate their own farms.
Yost and her husband, George, own and operate Nutra-Lix, a feed supplement company in Billings that distributes its product in six states and Canada.
Both the Yosts grew up in agriculture. George's family sold its ranch, while Karen's brother took over the family farm.
Karen Yost is halfway through her two-year term as American Agri-Women president. She previously spent two years as its first vice president and, after she's finished as president, will spend two years as past president.
As first vice president, Yost was heavily involved in recruiting new members, a goal that remains important to her as president.
Women who join American Agri-Women will discover they have passions in common with other members, she says.
Yost encourages women without direct ties to agriculture, but who want to learn more about it, to consider joining.
More information: www.americanagriwomen.org.
Tags: livestock, crops, agribusiness, updates


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Scream guitar pro by In Flames, added: February 27th, 2006
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WARNING: You are trying to view content from Ultimate-Guitar.com in an unauthorized application, which is prohibited. Please use an official Ultimate Guitar Tabs application for iPhone, iPad or Android to access legitimate chords, guitar, bass, and drum tabs from Ultimate-Guitar.com database. Type "ultimate guitar tabs" in Apple App Store's or Android Market's search to find the application.
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It has five stars now :)
Very good tab, just a few notes are off here and there, but you can figure them out on your own, and it doesn't really matter when your guitar is tuned that low. Plus, this has the little bridge/interlude thing at the end that a lot of tabs are missing. Great work!


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April 25, 2013
Culvert replacement will affect weekday recreation in the Blanchard Forest BlockTrails will be accessible by the Blanchard Lower Trailhead
OLYMPIA – The Washington State Department of Natural Resources (DNR) will replace old culverts and fix worn roads in the Blanchard Forest Block in Skagit County beginning May 6 through, approximately, July 1. Construction will take place Monday through Thursday, from 6:30 a.m. to 4 p.m. The road will be open to recreation use Friday through Sunday. Closures will be periodic and can be tracked through DNRs blog and social media sites.
During the weekday construction, the public can expect intermittent road closures for up to 10 hours and up to 20-minute delays otherwise. The Blanchard Lower Trailhead will be accessible throughout the process, allowing for trail access.
Map: http://www.dnr.wa.gov/Publications/amp_rec_blanchard_road_maintenance_4_2013.pdf
For updates on closure information, please visit DNR's blog: Recreation Alert in Blanchard Forest: Road maintenance will limit weekday access
Media Contact: Diana Lofflin, Recreation Program Communications Manager, 360-902-1169, firstname.lastname@example.com
# # #
DNR Communications & Outreach email@example.com


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Strangers to Ourselves: Discovering the Adaptive Unconscious by Timothy D. Wilson, Belknap Press, $27.95/£18.50, ISBN 0674009363
IN THE JOKE about two behaviourists chatting after sexual intercourse, one says to the other: "That was fine for you, but how was it for me?" This captures the core idea of behaviourism - that human beings should be seen as black boxes whose input and output, stimulus and response, are the only worthy objects of study. All internal states, experiences and hairy stuff like consciousness are best ignored.
Behaviourism passed away half a century ago, but the joke is now in for a renaissance.
Ever since the ban on discussing conscious awareness and other subjective features of human existence was lifted a little more than a decade ago, it has become more and more clear that most of human mental functioning is nonconscious. We experience, feel and think many ...
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