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JOURNAL OF CLINICAL MICROBIOLOGY, May 2007, p. 1581–1587
0095-1137/07/$08.00?0 doi:10.1128/JCM.01024-06
Copyright © 2007, American Society for Microbiology. All Rights Reserved.
Vol. 45, No. 5
Development of a Loop-Mediated Isothermal Amplification Assay
for Rapid Detection of BK Virus?
Bipin Raj Bista,1Chandra Ishwad,1Robert M. Wadowsky,2Pradip Manna,3Parmjeet Singh Randhawa,4
Gaurav Gupta,1Meena Adhikari,5Rakhi Tyagi,5Gina Gasper,1and Abhay Vats1*
Department of Pediatrics, Children's Hospital of Pittsburgh, 3705 Fifth Avenue, Pittsburgh, Pennsylvania 152131; Department of Pathology,
Children's Hospital of Pittsburgh, 3705 Fifth Avenue, Pittsburgh, Pennsylvania 152132; Department of Molecular Diagnostics,
ViraCor Laboratories, 1210 NE Windsor Drive, Lee's Summit, Missouri 640863; Department of Pathology, University of
Pittsburgh Medical Center, 200 Lothrop Street, Pittsburgh, Pennsylvania 152134; and Department of
Molecular Virology, Atharva BioSciences, New Delhi 110029, India5
Received 16 May 2006/Returned for modification 6 July 2006/Accepted 14 December 2006
Loop-mediated isothermal amplification (LAMP) is a novel method for rapid amplification of DNA. Its advan-
tages include rapidity and minimal equipment requirement. The LAMP assay was developed for BK virus (BKV),
which is a leading cause of morbidity in renal transplant recipients. The characteristics of the assay, including its
specificity and sensitivity, were evaluated. BKV LAMP was performed using various incubation times with a variety
of specimens, including unprocessed urine and plasma samples. A ladder pattern on gel electrophoresis, typical of
successful LAMP reactions, was observed specifically only for BKV and not for other viruses. The sensitivity of the
assay with 1 h of incubation was 100 copies/tube of a cloned BKV fragment. Additionally, a positive reaction was
visually ascertained by a simple color reaction using SYBR green dye. BKV LAMP was also successful for urine and
plasma specimens without the need for DNA extraction. Due to its simplicity and specificity, the LAMP assay can
potentially be developed for "point of care" screening of BKV.
BK virus (BKV) can cause renal allograft nephropathy
(BKVAN), which is now recognized as one of the major man-
ifestations of the various syndromes associated with polyoma-
viruses (4–6). Currently, 1 to 10% of renal transplant recipients
are diagnosed with BKVAN, which can lead to graft loss in 20
to 80% of patients (3, 7, 13, 19). Recent studies have also
suggested a viruria prevalence of approximately 30% and a
viremia prevalence of 7 to 10% in patients with renal trans-
plants (2, 4–6, 16, 19). Over the last several years, we devel-
oped and brought into clinical practice a quantitative real-time
(TaqMan) PCR assay for BKV (16, 19). We have also shown
that screening by monitoring of viral load in urine can predict
patients at risk for development of BKVAN (16). The corner-
stones of BKVAN management are an early diagnosis followed
by a reduction in immunosuppression with or without antiviral
therapy (4–7, 19). Thus, different PCR techniques have now
become a valuable tool for screening and monitoring for active
BKV infection (7, 16, 19). However, PCR assays require con-
siderable operator skill and expensive equipment. Rapid diag-
nostic tests that can be performed in the outpatient clinic
without the need for complex procedures (like DNA extrac-
tion) or expensive equipment can potentially improve our ca-
pability to screen for this important transplant complication.
Recently, a novel, specific, and rapid technique for amplifi-
cation of DNA under isothermal conditions was reported (14).
The technique, termed loop-mediated isothermal amplifica-
tion (LAMP), requires a set of conditions and primers different
from those used for PCRs (8, 12, 14, 15). LAMP assays have
now been reported for several pathogens (8, 9, 12, 14, 15, 18,
20). The reaction typically occurs over 30 to 60 min under iso-
thermal conditions with temperatures ranging from 60 to 65°C
and can be conducted with a simple heating block. Thus, the
thermal-cycling needs of a PCR are avoided. In this study, we
developed and characterized LAMP primers and amplification
protocols for a BKV assay (U.S. patents pending). We report the
details of this new technique and the assay characteristics.
Patient samples. Urine and blood samples obtained from renal transplanta-
tion patients and stored at ?80°C were utilized for development and testing of
BKV LAMP assays and for comparison with PCR assays. The patients signed an
informed consent, and the studies were approved by the Internal Review Board
of the University of Pittsburgh Medical Center and the Children's Hospital of
Pittsburgh (CHP). Anonymous samples were obtained from ViraCor Laborato-
ries and the microbiology laboratories of CHP for various viruses.
Viral DNA extraction. Viral DNA was extracted from blood samples by using
a QIAmp DNA mini kit (QIAGEN Inc., CA). A QIAmp viral RNA kit was used
to extract viral DNA from urine samples, as we have found that this method
provides a better yield for urinary viral DNA extraction than the use of a DNA
kit (16, 17). The DNA was finally eluted in a 60-?l volume of elution buffer and
stored at ?20°C until further use.
LAMP reaction. The LAMP reaction was conducted, following methods de-
scribed by Notomi et al. and Nagamine et al. with minor modifications (12, 14).
Primers specific for BKV were designed and synthesized (Integrated DNA Tech-
nologies, IA). The six primers consisted of one pair each of forward and reverse
outer primers (BKVTF3 and BKVTB3), forward and reverse inner primers
(BKVTFIP and BKVTBIP), and forward and reverse loop primers (BKVTloopF
and BKVTloopB). The LAMP reaction mixtures (final volume, 25 ?l) contained
these three primer pairs in a 1:8:4 ratio, i.e., 0.2 ?M each of the outer primers,
1.6 ?M each of the inner primers, and 0.8 ?M each of the loop primers. The
other components of the reaction mixture were 2.5 ?l of 10? Bst DNA poly-
merase reaction buffer (New England Biolabs, MA), 1 ?l of 8U/?l Bst DNA
polymerase (New England Biolabs Inc., MA), 2 mM of MgSO4(2 ?l), 5 ?l of
Betaine (Sigma-Aldrich, MO), and 5 ?l of a target sample. The Bst DNA
polymerase reaction buffer (1?) contained 20 mM Tris-HCl (pH 8.8, 25°C), 10
* Corresponding author. Mailing address: Department of Pediatrics,
Children's Hospital of Pittsburgh, 3705 Fifth Avenue, Pittsburgh, PA
15213. Phone: (412) 692-5182. Fax: (412) 692-7443. E-mail: abhay.vats
?Published ahead of print on 21 February 2007.
Page 2
mM KCl, 10 mM (NH2)SO4, 2 mM MgSO4, and 0.1% Triton X-100. The
reaction mixtures were incubated at a temperature of 63°C for various periods of
time, ranging from 30 to 120 min.
LAMP product detection. As part of the assay development, the LAMP prod-
ucts were initially detected by several methods, including electrophoresis using
agarose gel (1.2%) with UV light transillumination and photography using the
KODAK EDAS gel documentation system (Eastman Kodak Company, Roch-
ester, NY). A portable UV transilluminator was also used (FastGel system;
Cambrex BioSciences Inc., Rockland, ME) for LAMP product visualization. In
addition, the products were detected visually by a color change after addition of
SYBR green I dye to tubes containing LAMP products. Ten microliters of SYBR
green I dye (100?) (Cambrex BioSciences Inc., Rockland, ME) was added to
each tube containing LAMP products. The LAMP products were also detected
spectrophotometrically (SmartSpec 3000; Bio-Rad Laboratories, CA) at wave-
lengths of 494 to 521 nm. After the correlation was established between various
detection methods, the SYBR green dye method was used for evaluation of
clinical samples.
Amplification targets and LAMP equipment. BKV DNA was used to stan-
dardize the LAMP reaction and to determine the specificity of the assay. DNA
extracted from cytomegalovirus (CMV), Epstein-Barr virus (EBV), human her-
pesvirus 6 (HHV-6), adenovirus (Ad), herpes simplex virus 1 and 2 (HSV-1 and
HSV-2), varicella-zoster virus (VZV), and other polyomaviruses (simian virus 40
[SV40] and JC virus [JCV]) was used to determine the specificity of the BKV
LAMP reaction. CMV-, EBV-, HHV-6-, Ad-, HSV-1-, HSV-2-, VZV-, and
SV-40-positive patient blood or urine samples were obtained from the microbi-
ology laboratories of CHP and ViraCor. JCV was obtained from the American
Type Culture Collection (ATCC number 45027). A plasmid containing a cloned
target sequence of BKV (pBKVT3) was used to determine the sensitivity of the
reaction. The negative controls used for various reactions included no-template
controls and no-enzyme controls. The LAMP reaction was primarily carried out
with a heating block (LAB-LINE; Barnstead International, Dubuque, IA). The
reaction was also performed with conventional PCR (MyCycler; Bio-Rad Lab-
oratories, CA) and real-time PCR (Mini-Opticon; Bio-Rad Laboratories, CA)
Cloning of BKV DNA. To determine the sensitivity of the BKV LAMP assay,
we generated and quantitated a plasmid containing the target BKV sequence
(pBKVT3). Briefly, a 217-bp target DNA sequence spanning the region from
nucleotide position 4676 to nucleotide 4893 of the BKV genome was amplified
by PCR using the same outer primers as those used in the LAMP reaction
(BKVTF3 and BKVTB3). The amplified product was then cloned into a TOPO
cloning vector, using a TA cloning kit according to the manufacturer's instruc-
tions (Invitrogen, CA). The vector was used to transform XL1-Blue competent
Escherichia coli cells (Stratagene, CA). The transformed cells were incubated
overnight and the colonies with the insert (using blue-white distinction) further
grown. The cloned insert was isolated from the cells by use of a Fast Plasmid mini
kit (Eppendorf, NY). The presence of the positive clone was tested by digestion
of the plasmid DNA by EcoRI, followed by gel electrophoresis and sequencing.
The pBKVT3 clone was quantitated using UV spectrophotometry at 260 nm
(SmartSpec 3000; Bio-Rad Laboratories, CA). A series of 10? dilutions, span-
ning 10 to 10E7 copies/tube of the clone, was used to test the sensitivity of the
LAMP reactions.
Real-time PCR. The real-time PCR technique was used to quantify the BKV
DNA as previously described (16, 19) and to compare the sensitivities of the
LAMP and PCR assays. PCRs were performed using the Takara PCR protocol
(TakaraMirus Bio Inc., WI). The reaction mixtures (20 ?l) contained 10 ?l of
Takara master mix, 0.67 ?l each of 10 mM forward and reverse primers, 0.67 ?l
of 10 mM probe, and 5 ?l of the sample. Thermal cycling and quantitation were
performed using a Bio-Rad Mini-Opticon machine (Bio-Rad Laboratories, CA).
The reaction mixture was cycled as follows: 95°C for 30 seconds and 40 cycles of
two-step PCR, with each cycle consisting of 95°C for 1 s, followed by 60°C for 5 s,
with plate reading after each cycle. The primers and probe used for the real-time
PCR are located in the cloned plasmid (pBKVT3), and the sequences were as
follows: forward, GGACCCACCATTGCAGAGTTT; reverse, AGAGCCCT
TGGTTTGGATAGATT; probe, 6-FAM (6-carboxyfluorescein)-5?-AAGC
CAAACCACTGTGTGAAGCAGTCAAT 3?-TAMRA (6-carboxytetramethyl-
rhodamine). The real-time measurement of BK viral load in clinical specimens
also utilized an internal control. Each specimen was spiked with a universal
internal control (UIC) prior to DNA extraction. The UIC contains a partial gene
sequence for green fluorescence protein that is absent in viral-pathogen and
mammalian genomes. To assess for PCR inhibition, a separate real-time PCR
assay was performed with UIC-specific primers and probe. Real-time PCR in-
hibition was defined by the absence of UIC target amplification.
Assay design. The LAMP primers were selected from a
conserved region of the BKV genome as determined by whole-
genome sequencing of several viral strains obtained from dif-
ferent BKV-positive patient samples and are shown in Table 1.
The locations and sequences of the different primers are shown
in Fig. 1. All primers were designed by manual inspection of
TABLE 1. LAMP primers
Primer used for BKV LAMPSequence No. of bases
Outer forward and backward
GGACCCACCATTGCAGAGTTT (outer forward primer)
TCTTTGCCCAGATACCCTGT (outer primer)
Inner forward and backwarda
BKVTBIP (B1 ? B2c)
BKVTFIP (F1c ? F2)
Loop forward and backward
BKVTLoop F
BKVTLoop B
aThe F1c and B2c sequences are complementary and reverse to the F1 and B2 regions shown in Fig. 1 and are shown as underlined. The BKVTBIP primer had two
degenerate bases in three positions (W ? A/T and Y ? C/T).
FIG. 1. Segment of large T-antigen gene sequence (nucleotide po-
sitions 4676 to 4893) showing the locations and orientations of various
primers used in the BKV LAMP assay.
Page 3
the sequence data. Briefly, the outer primers (F3 [BKVTF3]
and B3 [BKVTB3]) were located outside two inner primers
(labeled F2 [BKVTF2] and B2 [BKVTB2]) in the large-T-
antigen gene region of the BKV genome. The forward internal
primer (BKVTFIP) comprises a fusion primer with the com-
plementary F1 sequence (F1c) and the direct F2 sequences
(14). Similarly, the reverse internal primer (BKVTBIP) com-
prises the direct B1 sequence and the complementary B2 se-
quence (B2c) (Table 1). To increase the amplification effi-
ciency, two loop primers (forward [BKVTloopF] and reverse
[BKVTloopB]) were also designed. The loop primers signifi-
cantly improved the performance of the assay and led to a
considerable reduction in the time required for amplification
of the target. The LAMP reaction was carried out at a constant
temperature of 63°C and generally was positive after various
periods of time (30 to 120 min) with the addition of loop
primers to the reaction mixture. Without the use of loop prim-
ers, the same reactions required periods 4 to 5 times longer
(i.e., a 30-min reaction required over 2 or 3 h). Also, several
samples with lower viral loads could not be amplified at all
without the addition of loop primers.
Assay specificity and sensitivity. A positive BKV LAMP
reaction typically required incubation for 60 min and revealed
a ladder pattern with many bands of different sizes on agarose
gel electrophoresis (Fig. 2A, BKV lane). The specificity of the
BKV LAMP assay was evaluated by testing the assay on several
DNA viruses that can be seen in transplant patients (i.e., EBV,
CMV, HHV-6, Ad, HSV-1, HSV-2, and VZV) and the two
related polyomaviruses (i.e., JCV and SV40). No ladder pat-
tern was seen with the other (non-BK) viruses or for the neg-
ative control (Fig. 2A and B). The sensitivity of the LAMP
reaction was determined using 10? serial dilutions of the
pBKVT3 clone. The BKV LAMP sensitivity was found to be
100 copies per tube (Fig. 2C, lane 7) when an incubation
period of 60 min was employed.
Detection of LAMP products by alternate methods. Besides
gel electrophoresis, we also determined two additional meth-
ods, i.e., visual inspection and spectrophotometry, to detect a
positive reaction. Upon addition of the SYBR green I dye to
tubes after the LAMP reaction, the color changed to yellowish
green in a positive reaction (Fig. 3A, tube 1) and remained
reddish orange (the color of the unbound dye) in the negative
reactions (Fig. 3A, tube 2). Although the intensities of the
color changes appeared to be similar for a range of viral loads,
the color changes occurred earlier in samples with higher
loads. All the samples that were positive by gel electrophoresis
were also positive by visual detection of color change (and vice
versa). The visual detection of a positive reaction was further
improved by using UV light from a conventional as well as a
portable blue light transilluminator, which demonstrated a
bright green fluorescence in positive reactions (Fig. 3B and C).
Finally, the positive reaction was also detected by a spectro-
photometer in a quantitative manner.
LAMP assay with clinical samples. Detection of BKV DNA
by LAMP was done with 49 clinical blood and urine samples
obtained from renal transplant patients. Real-time PCR assays
for BKV were also done with the same samples for compari-
son. As there was a good correlation between various detection
methods, positive-reaction detection with clinical samples was
performed by visual observance of color change with SYBR
green and UV transillumination. A summary of the results and
the comparison between real-time PCR and LAMP is shown in
Table 2. Plasma samples required approaches slightly different
from those used for urine samples for LAMP to work success-
fully as described below.
Plasma samples. We analyzed 21 plasma samples from 18
different patients, and four of the plasma samples belonged to
a single BKVAN patient (no. 18). These four samples were
obtained over a period of 3 months and showed loads ranging
from 2,264 to 333,200 copies/ml. LAMP was performed with
extracted as well as unextracted plasma samples under two
separate conditions, i.e., after heating at 99°C for 10 min and
without heating (i.e., samples freshly obtained without any
processing). The unprocessed and unextracted plasma samples
did not give positive reactions with LAMP. However, the same
samples were successfully amplified by LAMP after the plasma
was heated. Approximately 15 to 20 ?l of supernatant was
obtained after 50 ?l of plasma was heated, of which 5 ?l was
used for each reaction (Fig. 4A). The LAMP assay was nega-
tive for samples that were negative by real-time PCR and also for
three samples with viral loads of ?1,500 copies/ml (Table 2). The
samples with viral loads of ?5,000 copies/ml required the LAMP
reaction to be performed for a longer period (120 min).
FIG. 2. Specificity and sensitivity of the BKV LAMP assay. (A) The
specificity of the BKV LAMP assay is shown in the positive reaction,
visible as a ladder-like pattern in lanes with BKV DNA only. A neg-
ative reaction was obtained when the target of amplification was other
non-BKV DNA viruses, i.e., EBV, CMV, Ad, HHV-6, HSV-1, HSV-2,
VZV, or no-target control (NTC). (B) The LAMP reaction was neg-
ative for two related polyomaviruses, JCV and SV40, and amplified
only BKV. (C) The BKV LAMP reaction was carried out with the
serial dilutions of cloned BKV plasmid (pBKVT3), and the sensitivity
of the reaction was determined to be 100 copies per tube.
Page 4
Urine samples. LAMP was performed with 28 urine samples
from 27 patients, including 8 renal transplant recipients with
asymptomatic viruria, 14 patients with biopsy-confirmed
BKVAN, and 5 patients with no viruria. We initially analyzed
the urine samples with and without (i.e., freshly obtained with-
out any processing) DNA extraction from 10 patient samples
(7 viruria and 3 BKVAN). Unlike for plasma, BKV LAMP
performed successfully even when freshly voided (unprocessed)
urine was added directly to the reaction mixture (Fig. 4B). The
reactions were positive for unprocessed urine samples from
four patients whose urine viral loads were ?50,000 copies per
ml (Fig. 4B). Interestingly, the assay was negative for two
samples (Fig. 4B, lanes 1 and 3) with viral loads of approxi-
mately 60,000 and 40,000 copies/ml even after incubation for
up to 2 h. All 14 BKVAN patients were positive by the LAMP
assay performed with unprocessed urine, including a patient
with a low viral load of 30,500 copies per ml (Table 2). These
results show that the sensitivity of LAMP is approximately
100,000 copies/ml for urinary samples, with variable perfor-
mance at lower urinary viral loads.
In recent years, the detection of BK viremia and viruria by
real-time PCR has become an important tool for identification
of patients at risk for developing BKVAN (4, 6, 7, 19). Despite
TABLE 2. Comparison of LAMP and real-time PCR results for
clinical plasma and urine samplesa
Sample no.
Disease status
BKV load
(real-time PCR)
(no. of copies/ml)
No BKV
No BKV
No BKV
Healthy control
Healthy control
Healthy control
Healthy control
Healthy control
Asympt viruria
Asympt viruria
Asympt viruria
Asympt viruria
Asympt viruria
Asympt viruria
Asympt viruria
Asympt viruria
No BKV
No BKV
No BKV
No BKV
No BKV
aAsympt viruria, asymptomatic viruria; neg, negative; pos, positive.
FIG. 3. Visual detection of LAMP products. (A) The BKV LAMP
products were detected after addition of SYBR green I dye. The tube
with a positive reaction (tube 1) shows a color change to yellowish
green, which can be distinguished from the reddish orange color of a
negative reaction. (B) The same positive sample, when visualized un-
der UV transillumination, shows a bright green fluorescence in tube 1
(positive reaction) compared to what is observed for tube 2 (negative
reaction). (C) A portable blue light illuminator that can be used in a
clinic setting for transillumination of LAMP products. A 0.5-ml PCR
tube is placed on it to show the relative size of the transilluminator.
Page 5
the clinical utility of PCR-based techniques, they have the
inherent disadvantage of being time-consuming as well as re-
quiring considerable operator skill and expensive equipment.
We demonstrate that the LAMP technique can be used to
amplify BKV DNA under isothermal conditions with high
specificity and efficiency. It has a minimal equipment require-
ment and can be accomplished in 1 hour or less. Moreover, we
have also shown that the LAMP reaction can be performed
even with unprocessed urine samples and minimally processed
(i.e., heat-treated) plasma samples without the need for DNA
extraction. Thus, this technique can potentially be developed
to screen for virus in "point of care" settings, such as outpa-
tient clinics.
The LAMP method is based on autocycling strand displace-
ment DNA synthesis performed by the Bst DNA polymerase
enzyme and was first described by Notomi et al. (14). The
principle underlying LAMP is complex, and excellent details of
the technique are available in two recent publications (12, 14).
Briefly, the LAMP assay requires isothermal strand displace-
ment and the amplicons are stem-loop DNA structures with
several inverted repeats of the target. The amplicons are
formed after multiple rounds of DNA amplification and have
structures with multiple loops. Typically, LAMP utilizes four
specifically designed primers (two pairs of outer and inner
primers) and isothermal Bst DNA polymerase, which has
strand displacement activity. Nagamine et al. reported a fur-
ther improvement by introducing another set of primers, called
loop primers, in the reaction (12). Loop primers hybridize to
the stem-loops that are formed in the initial phases of LAMP
and act by providing additional priming for the DNA polymer-
ase for strand displacement (12). The use of these additional
two primers (a total of six primers) further improves the am-
plification efficiency of the reaction, as originally reported by
Nagamine et al. and also confirmed in our studies. The ampli-
cons are typically seen as multiple bands on agarose gel elec-
trophoresis, in contrast to the single band seen with PCR.
An important advantage of LAMP is that the thermal-cy-
cling needs of the PCR method are completely avoided (12).
Also, the LAMP assay produces a large amount of amplified
product, resulting in easier detection by visual inspection, by
observation of either an increase in turbidity caused by gener-
ation of magnesium pyrophosphate or a color change after
addition of SYBR green I dye (10, 11, 12, 14). The change in
turbidity can also be measured quantitatively and in real-time
mode by using a real-time turbidimeter (8, 9, 15). However, the
increase in turbidity can be difficult to appreciate and we found
it difficult to detect a change in turbidity visually in our exper-
iments. However, visual detection of a red-to-green color
change with SYBR green I was easily accomplished and always
correlated with gel electrophoresis findings. Our ability to vi-
sually detect a positive reaction was significantly improved by
using UV transilluminators, including a portable blue light
transilluminator that recently became available and can be
useful in developing the assay for "point-of-care applications"
settings (Fig. 3C). One relative limitation of the LAMP assay is
that the color change appears at a visual level to be an all-or-
none phenomenon (i.e., a "yes" or a "no") and did not behave
in a semiquantitative manner, as the various shades of green
for different viral loads were not easily visually distinguishable.
Although we did not intend to design a quantitative or semi-
FIG. 4. BKV LAMP with plasma and urine samples from renal trans-
plant patients. (A) Four plasma samples from a single patient with dif-
ferent viral loads assayed with and without heating of samples. The
LAMP reaction was negative in unprocessed and unheated plasma sam-
ples (lanes 1 to 4) and positive in the plasma only after heat treatment of
the same samples for 10 min (lanes 5 to 8) or after DNA extraction
(CTRL, lane 9). Lane 1, 2,264 copies/ml; lane 2, 333,200 copies/ml; lane
3, 50,480 copies/ml; lane 4, 157,200 copies/ml. Lanes 5 to 8 show the same
four samples after heat treatment. Lane 5, 2,264 copies/ml (heated
plasma); lane 6, 333,200 copies/ml (heated plasma); lane 7, 50,480
copies/ml (heated plasma); lane 8, 157,200 copies/ml (heated plasma);
lane 9, positive control with extracted DNA (333,200 copies/ml).
(B) The LAMP reaction was carried out with unextracted, freshly
voided urine samples from 10 renal transplant patients. The corre-
sponding BKV DNA copies were as follows: lane 1, 58,000 copies/ml;
lane 2, 6,000 copies/ml; lane 3, 40,000 copies/ml; lane 4, 3.4E?9 copies/
ml; lane 5, 1.7E?9 copies/ml; lane 6, 54,000 copies/ml; lane 7, 110,000
copies/ml; lane 8, 390,000 copies/ml; lane 9, 52,000 copies/ml; lane 10,
20,000 copies/ml. (C) The BKV LAMP reaction was carried out with
DNA extracted from the same urine samples as those shown in panel
B. Note that the reaction was now strongly positive in lane 7 and faintly
positive in lane 10, in addition to lanes 4, 5, 6, and 9 in panel B.
Page 6
quantitative LAMP assay, the time required to develop a color
change with SYBR green can possibly be used to get a semi-
quantitative idea of the viral load. The color change occurs
earlier at higher viral loads (i.e., ?30 min incubation for viral
loads of ?10E7 copies) and takes longer for lower loads (60
min for 10,000 to 100,000 copies/ml).
The LAMP assay is more complex to design than PCR
assays. However, once developed, LAMP is much easier to
perform than PCR or real-time PCR and requires a simple
addition of unprocessed urine or plasma to a tube containing a
mixture of primers, enzyme, and buffers, followed by warming
for 30 to 60 min at 60 to 65°C. Hence, the hands-on time of
approximately 1 h or less for LAMP can be comparable to or
even significantly shorter than that required for PCR or real-
time PCR. Another advantage of LAMP is that, in contrast to
PCR, it requires no DNA extraction, which can be a major
obstacle in widespread employment of DNA amplification as-
says. Our LAMP assay performed successfully without DNA
extraction for both urine and plasma samples. Unprocessed,
freshly voided urine is considered to contain PCR inhibitors
but was successfully tested with BKV LAMP assays. Although
there was a loss of some sensitivity with the use of unprocessed
samples (versus what was found for extracted DNA samples),
the assay could still be very useful in clinical situations. The
reasons for the variable performance of the assay for viral
loads of less than 100,000 copies/ml of urine are not clear but
may be multifactorial. One possibility is that the LAMP assay
demands more integrity of the target, owing to the many prim-
ers that need to be annealed to the different regions, than
PCR. Since the LAMP assays were tested on stored samples, it
is possible that there may have been partial degradation of the
target DNA in the region of LAMP primers but not in the
region that is amplified by PCR primers. Another possibility is
the presence of urine-specific inhibitors of LAMP reaction.
The patients with false-negative urine LAMP results did not
have any obvious clinical differences from other patients and
also did not have any proteinuria, which can possibly act as an
inhibitor. However, various drugs, metabolites, chemicals, and
dietary agents are excreted in urine, and some of these may
affect urinary LAMP more than plasma LAMP assays. Further
refinements of technique are clearly needed to improve the
sensitivity and the semiquantitative aspects of the LAMP as-
says as well as to address the issues related to as-yet-unrecog-
nized urinary "inhibitors." Also, additional studies, including
validation of visual testing with a larger number of clinical
samples and proficiency testing, will be required before these
assays can be employed in routine clinical laboratories.
BKVAN is emerging as an important cause of renal allograft
dysfunction and can resemble acute rejection (AR) both clin-
ically and histologically (3, 6, 7, 13). Differentiation between
BKVAN and AR is important, however, because the treat-
ments for the two conditions are diametrically opposite. Sev-
eral studies have shown that BK viruria is a requirement for
development of BKVAN and generally precedes the appear-
ance of viremia and full-blown nephropathy by several weeks
to months. BKV DNA detection in urine by PCR is also much
more sensitive than viral detection by urinary cytology (decoy
cells) and also viral detection by immunohistochemistry or in
situ hybridization on allograft biopsy (2, 3, 16). However,
asymptomatic BK viruria can be seen in up to 30% of kidney
transplant patients and only approximately 6 to 10% of these
patients develop full-blown BKVAN (13, 19). The presence of
a high viral load is now considered to be more correlated with
risk for development of BKVAN than just the presence of virus
in urine (16, 17). Our previous studies have shown that viral
loads of ?10E7 copies/ml in urine and ?10,000 copies/ml in
blood are reasonable threshold loads above which a renal
transplant patient is more likely to have or develop BKVAN
(16, 17, 19). BKV LAMP showed consistent performance in
our studies at urine loads of ?10E5 copies/ml and for ?2,000
copies/ml of plasma even when performed with unextracted
samples. These detection limits are well below the viral loads
that are considered to be clinically significant. Hence, this assay
could easily be used in point-of-care settings to detect high-risk
patients with viral loads above the critical levels. Demonstra-
tion of BKV in urine and blood can alert a clinician to the
possibility of BKVAN in a patient presenting an asymptomatic
rise in serum creatinine, which is most often attributed to AR
in renal transplant recipients.
In summary, we report our early experience with the devel-
opment and performance characteristics of a BKV LAMP as-
say. It is a novel technique that can possibly be used for rapid
diagnosis of BKV infection not only in laboratories but also in
an outpatient clinic setting. A reduction in immunosuppression
is now considered the first-line therapy for BKV infections in
renal transplant recipients (1, 4, 7). However, physicians cur-
rently depend on PCR results for BKV detection, which might
take 2 to 3 days or longer to arrive in many clinic settings. Thus,
the LAMP assay for BKV can potentially be used to guide
therapeutic decisions in outpatient clinics itself, especially if a
urine sample becomes positive after a short, 30-min incubation
and is accompanied by a plasma sample that shows positivity,
suggesting that the patient is more likely to have a clinically
significant viral load. Further large-scale studies for determi-
nation of the sensitivity, specificity, and clinical utility of this
new method will be needed before this method can find wider
clinical applicability.
This work was supported by an NIH award (R01 AI060602) to A.V.
1. Brennan, D. C., I. Agha, D. L. Bohl, M. A. Schnitzler, K. L. Hardinger, M.
Lockwood, et al. 2005. Incidence of BK with tacrolimus versus cyclosporine
and impact of preemptive immunosuppression reduction. Am. J. Transplant.
2. Drachenberg, C. B., J. C. Papadimitriou, H. H. Hirsch, R. Wali, C. Crowder,
J. Nogueira, et al. 2004. Histological patterns of polyomavirus nephropathy:
correlation with graft outcome and viral load. Am. J. Transplant. 4:2082–
3. Drachenberg, C. B., H. H. Hirsch, E. Ramos, and J. C. Papadimitriou. 2005.
Polyomavirus disease in renal transplantation: review of pathological find-
ings and diagnostic methods. Hum. Pathol. 36:1245–1255.
4. Hariharan, S. 2006. BK virus nephritis after renal transplantation. Kidney
Int. 69:655–662.
5. Hirsch, H. H. 2005. BK virus: opportunity makes a pathogen. Clin. Infect.
Dis. 41:354–360.
6. Hirsch, H. H., and J. Steiger. 2003. Polyomavirus BK. Lancet Infect. Dis.
7. Hirsch, H. H., D. C. Brennan, C. B. Drachenberg, F. Ginevri, J. Gordon,
A. P. Limaye, et al. 2005. Polyomavirus-associated nephropathy in renal
transplantation: interdisciplinary analyses and recommendations. Transplan-
tation 79:1277–1286.
8. Hong, T. C., Q. L. Mai, D. V. Cuong, M. Parida, H. Minekawa, T. Notomi,
et al. 2004. Development and evaluation of a novel loop-mediated isother-
mal amplification method for rapid detection of severe acute respiratory
syndrome coronavirus. J. Clin. Microbiol. 42:1956–1961.
Page 7
9. Ihira, M., T. Yoshikawa, Y. Enomoto, S. Akimoto, M. Ohashi, S. Suga, et al.
2004. Rapid diagnosis of human herpesvirus 6 infection by a novel DNA
amplification method, loop-mediated isothermal amplification. J. Clin. Mi-
crobiol. 42:140–145.
10. Mori, Y., K. Nagamine, N. Tomita, and T. Notomi. 2001. Detection of
loop-mediated isothermal amplification reaction by turbidity derived from
magnesium pyrophosphate formation. Biochem. Biophys. Res. Commun.
11. Mori, Y., M. Kitao, N. Tomita, and T. Notomi. 2004. Real-time turbidimetry
of LAMP reaction for quantifying template DNA. J. Biochem. Biophys.
Methods 59:145–157.
12. Nagamine, K., T. Hase, and T. Notomi. 2002. Accelerated reaction by loop-
mediated isothermal amplification using loop primers. Mol. Cell. Probes
13. Nickeleit, V., and M. J. Mihatsch. 2004. Polyomavirus allograft nephropathy
and concurrent acute rejection: a diagnostic and therapeutic challenge.
Am. J. Transplant. 4:838–839.
14. Notomi, T., H. Okayama, H. Masubuchi, T. Yonekawa, K. Watanabe, N.
Amino, et al. 2000. Loop-mediated isothermal amplification of DNA.
Nucleic Acids Res. 2:E63.
15. Poon,L.L.,B.W.Wong,E.H.Ma,K.H.Chan,L.M.Chow,W.Abeyewickreme,
N. Tangpukdee, K. Y. Yuen, Y. Guan, S. Looareesuwan, and J. S. Peiris. 2006.
Sensitive and inexpensive molecular test for falciparum malaria: detecting Plas-
modium falciparum DNA directly from heat-treated blood by loop-mediated
isothermal amplification. Clin. Chem. 52:303–306.
16. Randhawa, P., A. Ho, R. Shapiro, A. Vats, P. Swalsky, S. Finkelstein, et al.
2004. Correlates of quantitative measurement of BK polyomavirus (BKV)
DNA with clinical course of BKV infection in renal transplant patients.
J. Clin. Microbiol. 42:1176–1180.
17. Randhawa, P., R. Shapiro, and A. Vats. 2005. Quantitation of DNA of
polyomaviruses BK and JC in human kidneys. J. Infect. Dis. 192:504–509.
18. Suzuki, R., T. Yoshikawa, M. Ihira, Y. Enomoto, S. Inagaki, K. Matsumoto,
et al. 2006. Development of the loop-mediated isothermal amplification
method for rapid detection of cytomegalovirus DNA. J. Virol. Methods
19. Vats, A., R. Shapiro, P. Singh Randhawa, V. Scantlebury, A. Tuzuner, M.
Saxena, M. L. Moritz, T. J. Beattie, T. Gonwa, M. D. Green, and D. Ellis.
2003. Quantitative viral load monitoring and cidofovir therapy for the
management of BK virus-associated nephropathy in children and adults.
Transplantation 75:105–112.
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Detection of human herpesvirus 7 DNA by loop-mediated isothermal am-
plification. J. Clin. Microbiol. 42:1348–1352.


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25 Reviews
Average Customer Review
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5.0 out of 5 stars Who let the pigs out?
This hilarious book will have your kids rolling on the floor with laughter. It is now being released in board book form so little hands can experience the happiness of handling and cuddling their own-sized book. The text and illustrations are modified from the original but will not be missed by a brand new gang of potential Munsch lovers.
Megan's dad asks her...
Published 12 months ago by Storywraps
4 of 4 people found the following review helpful
2.0 out of 5 stars Not his best
My 3 1/2 year old daughter is a huge Munch fan and likes this one well enough, but it's my least favourite. She tends to memorize books and quote them constantly, and I'm not thrilled with the "hey, you dumb...." mantra. This is kind of minor, but how was it that the pigs showed up at school after Megan locked them back in at home? If you're wanting to add to your Munch...
Published on July 7 2002
Most Helpful First | Newest First
4 of 4 people found the following review helpful
2.0 out of 5 stars Not his best,
By A Customer
My 3 1/2 year old daughter is a huge Munch fan and likes this one well enough, but it's my least favourite. She tends to memorize books and quote them constantly, and I'm not thrilled with the "hey, you dumb...." mantra. This is kind of minor, but how was it that the pigs showed up at school after Megan locked them back in at home? If you're wanting to add to your Munch collection, he's written better. Alligator Baby and Thomas's Snowsuit are fun without the insults and a little more logically consistent.
4.0 out of 5 stars I love Robert Munsch, but I don't always love the board book editions,
Mary Lavers (in Canada) "CozyLittleBookJournal" (Dartmouth, NS, Canada) - See all my reviews
This review is from: Pigs (Board book)
I adore Robert Munsch. I think he's a masterful storyteller and his long-time collaboration with illustrator Michael Marchenko has produced some amazing children's classics.
Sometimes, however, when his books are condensed to board book form, they lose some of their oomph. The best parts are edited out, or they're oversimplified, or the magic is somehow not there.
Pigs, however, retains all of its "Pigness" even in the shorter format. My only problem with it is that I'm not sure this particular story *needed* to be made into a board book.
The board book format is presumably so it's easier for little hands to handle, so that younger children can access the story. I'm not sure Pigs is best suited for younger kids. It's one of the Robert Munsch stories that has characters being a bit rude to each other, with the constant refrain of "Hey you dumb pigs!". This may be fine for school-age children, but a lot of parents and teachers don't like reading that kind of impolite phrase to very young children.
This review was first posted on my blog, Cozy Little Book Journal, and is based on a digital review copy of the board book edition that I received through NetGalley.
5.0 out of 5 stars Who let the pigs out?,
Storywraps "Marilyn" (Ontario, Canada) - See all my reviews
This hilarious book will have your kids rolling on the floor with laughter. It is now being released in board book form so little hands can experience the happiness of handling and cuddling their own-sized book. The text and illustrations are modified from the original but will not be missed by a brand new gang of potential Munsch lovers.
Megan's dad asks her to feed the pigs but to be careful not to open the gate because pigs are smarter than you think. Megan promises she will not allow that to happen and heads off outside to the pigpen. Her observation after leaning over the gate and giving the piggies a going over with her eyeballs concludes that pigs are the dumbed looking animals that she has ever seen. Little by little her nose gets closer to the specimens and the gate creeps open a little more each time she leans in. The pigs watch her, wait patiently and at the precise perfect time they all stampede out of the gate. Oh oh!
They make a beeline or should I say piggyline to the house and cause havoc and chaos inside. "Get these pigs out of here! yelled her father. Megan yelled, "HEY YOU DUMB PIGS!" The pigs jumped up-wap, wap, wap! - and ran out the door." Then they zoom off to Megan's school to cause mayhem and disturbances there. When it's time to head for home and the bus pulls up for pick up, guess whose driving the bus? You guessed it...all aboard the piggy bus....and the pigs squeal and chew (the bus seats) and party ..... all the way home. Could Megan restrain herself from ever repeating that faux pas again? You will laugh at loud at the last page where that question just may be answered for you. Oh oh!
The illustrations are magnificent! Spot on! Amazing! Colourful, full of expression and emotion, action-packed and cartoonish. There is so much going on in each picture that it is worthwhile visiting the book again and again just to explore the illustrations and appreciate all of Martchenko's work. The teamwork of the best text-writer and the best creative artist makes this book a must have and I am so glad they downsized it so little pint-sized humans can start early on becoming a Robert Munsch/Michael Martchenko fan. Yea and they are both Canadian! Hurray for Canada!
1 of 1 people found the following review helpful
5.0 out of 5 stars GREAT BOOK!!,
By A Customer
My teacher read PIGS to my class. A girl said that pigs aren't smart. I think that pigs are smart! When I grow up if decide to be a book publisher, I would publish a book like this.
5.0 out of 5 stars Pigs-Desription,
By A Customer
Pigs was an exciting and interesting book to do an author study on. My friends and I had a very fun experience acting this book out for a play. We got to show everyone the exciteness of this book. I know Robert Munsch had a very exciting experience writing this book. I recommend for kids to read this book, because not only is it a book for fun but it also teaches a moral. The moral is that you should listen to your elders. This book is better than other books because it is exciting and thrilling. I would rate this book 5 stars!!!
4.0 out of 5 stars Great for little hands,
Verified Purchase(What's this?)
If you child loves animals and silly stories they will love this book.
My toddler loves the Robert Munsch books and the prefers the mini books as they fit perfectly in her hands and are easy for her to turn the pages no matter where she is. Great for parents too for travelling and takes up no room. Can throw the book in your pocket or purse as you are running out the door.
4.0 out of 5 stars We Love THis Book,
"musical_theatre_major" (Austin, Texas) - See all my reviews
I read this book yesterday to my 3-year-old pre-k class today during our afternoon circle time. This story had their attention from beginning to end. I have to add Robert Munsch to our list of favorite authors. Pigs kept my class of 24, that is not a typo,twenty-four 3-year-olds entertained 2 times in a row! The want to hear it again tomorrow for our morning circle time.
5.0 out of 5 stars WONDERFUL read-aloud...,
Jennifer Churchwell (Grapevine, TX United States) - See all my reviews
Being a substitute teacher isn't always the easiest job and sometimes you have a lot of time to fill. I frequently used this book during those down times and it was ALWAYS a hit with the grade school set, they loved to make the pig sounds in the book. A MUST HAVE for any grade school teacher or a parent with grade school aged children!
4.0 out of 5 stars Cute book but it is very small,
Luke Harris (Ontario, Canada) - See all my reviews
Well, clearly I missed the (mini book) portion of this ad. The book was presented to me when I was purchasing another item. Naturally the great priced induced me to buy it. When I received it I could not believe how small the book was. My kids enjoy the story but have a hardtime getting into such a small book.
2.0 out of 5 stars "dumb" through out the book,
Verified Purchase(What's this?)
This review is from: Pigs (Board book)
The word "dumb" written throughout the book, and although the meaning of the story is that pigs are not dumb, I did not feel like having my 2 1/2 year old going to daycare and calling the other kids "dumb" since it would have been her nearly learned word.
Most Helpful First | Newest First
Pigs by Robert Munsch (Library Binding - Feb. 1 1992)
Used & New from: CDN$ 0.01

subset: commoncrawl


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6:53 am
Mon April 16, 2012
NRA Gets In On The Zombie Craze
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The National Rifle Association's annual convention featured a display of shooting targets featuring zombies. Firing ranges across the country are offering zombie-themed shooting events. Sales of zombie targets are booming.


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psychological adjustment. Now she's dependent on the guy. A few more attacks like that would turn her into his slave. Devious ******* is devious. I like his style ^.6
40. Love My Way, chapter posted 6/27/07
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"…you finally being how you want to be…" were his exact words.
"Your 'imagined' self was too attached to Michael. I actually have a chance with the 'real' you." was what he meant.
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wow that's actually…kinda harsh…


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They dug out with a subtle tactical switch by coach Steve Fisher, who had unsuccessfully toyed with his rotation for most of the night. He inserted freshman point D'Erryl Williams and moved Thames to the 2, which did two things. It gave the Aztecs an aggressive defensive presence on the perimeter from the 6-2, 200-pound Williams; and it allowed them to run plays to get Thames shots.
Thames (19 points) responded with a pair of 3s off down screens and a driving layup, and Miller started frantically calling timeouts to dull a suddenly inspired crowd.
The Aztecs made it close. Just not close enough.
Last year, Nick Johnson beat the Aztecs with a flying block on the game's last play in the final of the Diamond Head Classic in Honolulu. This time he did it with his scoring, finishing with a game-high 23 points despite playing the closing minutes with four fouls.
"He did things that your leader does," Fisher said. "When he needed a play, he made it for them down the stretch, whether it was free throws or the drive to the rim."
Other than the 19 points from both O'Brien and Thames, SDSU got 12 from Winston Shepard. No one else, though, had more than five. Davis' final line: 1-of-7 shooting, no free throws, two points, four rebounds, two turnovers, 33 minutes.
"We couldn't make that next play," Fisher said. "Good teams don't let you make that next play. They're good. They're big and long. You want to win but you want to say, 'We are going to be better as the result of playing the game.' And I do believe we will."


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Tag: EWRView All Tags
La Compagnie / Business Class Travel / Business Class Jets / EWR / LTN / Flight Sales / New Airline Routes / → All Tags
La Compagnie's Intro Pond-Hopping Offer is Just $1,007 Round-Trip
Last week we told you there was a French way to get from London to New York and now today, you can actually make your reservations.
Flights are now open for La Compagnie, the French all-business class airline, for their new London Luton (LTN)-Newark International Airport (EWR) route. The first flight will leave Newark on April 24. Right now, there are only three flights a week available--on Mondays, Fridays and Sundays--although a rep for La Compagnie assures us there will be four flights a week with a Wednesday departure. La Compagnie hopes to have daily flights by the end of 2015.
And we hope so too, if rates can stay as good as the intro fare which is being publicized at $1,007 round-trip. (Yes, US Dollars. Yes, taxes and fees included.) The deal is valid through April 24 for travel from April 24 to June 30.
La Compagnie / Business Class Travel / Business Class Jets / EWR / Business Class Amenities / New Airline Routes / → All Tags
Take Yourself from London to New York, the French Way, on La Compagnie
Last June, we learned that a new all-business class jet would by flying in the skies from Paris-Charles de Gaulle to Newark.
Its name? La Compagnie. Its philosophy? To be a "French boutique airline for people who like the freedom of business class travel without the business class price tag." We like French things, biz class and non-biz classes prices, so we're thinking we could be down to try it out, especially now that the airline is adding another route--London Luton to Newark-- on March 29.
But the all-important question remains--what are the seats like?
The Boeing 757-200 plane flown by La Compagnie has 19 rows and 74 all-business class seats. Here are the specifics on the seats and the amenities that the biz class travelers will get:
Frequent Flyer Miles / United Airlines / United / Airline Loyalty Programs / Dreamliner / Shopping Travel / Travel Tips / EWR / Airports / → All Tags
5 Ways to Spend United MileagePlus Miles and Get More Than a Glass of Wine
A few weeks ago, United began trialing a program at Newark Airport. It essentially allowed frequent flyers to redeem those hard-earned MileagePlus miles for food in some restaurants in the terminal. While it might be cool to just swipe your boarding pass and pay from your account, at about 1,000 miles for a glass of wine, the conversion rates aren't the most favorable.
This got us thinking that there are plenty of other United products better worth the miles than an overpriced meal in an overpriced airport restaurant...
United Airlines / United / Airlines / Airline News / EWR / Airports / Airport News / iPads / Travel Tech / Tech Travel / → All Tags
United Airlines Adding iPads to Each and Every Seat at EWR
United Airlines is offering some upgrades and improvements over at Newark Airport, but unfortunately it has nothing to do with the terminal—it's all about the technology.
The folks over at the airline have just made a pretty big order over at the Apple Store, as they're spending $120 million on new iPads and other gadgets to modernize bits and pieces of the airport experience. It sounds like the focus will be within Terminal C, as airline officials certainly recognize that their home at the airport is a little tired and certainly less than welcoming. We're not so sure that travelers can be tricked by a little touch screen trendiness, but we shall see.
Health Travel / Travel Safety / Africa Travel / Ebola / Airports / Airport News / JFK / EWR / ORD / IAD / ATL / Airport Security / → All Tags
US Limits Potential Ebola Travel to Just Five Airports
We've made a sustained effort to calm you down over Ebola. As the Internet will be more than happy to explain, more Americans have been married to Kim Kardashian than have died of Ebola. So as we move forward in this post, let's keep eventhing in perspective.
Have you heard, though, that all of the TSA stations in Cleveland International had to be disinfected inch by inch because an Ebola patient passed through just one of them on the way to Texas? It was simply a precaution of course - the literal actual quote from the agency's spokesman was that "it's nothing official" but rather just "something that our folks wanted to do" - but it happened and it brings up a good point. Ebola is spreading beyond West Africa partly because of air travel, and even the suggestion of Ebola is enough to bring anything its associated with to a grinding halt. So isn't Ebola eventually going to grind air travel to a halt?
Health Travel / Travel Safety / Africa Travel / Ebola / Airports / Airport News / JFK / EWR / ORD / IAD / ATL / Airport Security / → All Tags
US Airports Begin Extra Health Checks for Ebola
Whether you realize it or not, international airports are screening for health issues of incoming passengers. Typically it's just trained officials eyeballing the stream of arriving passengers, sometimes with the help of temperature sensors, looking for any telling signs of deteriorating health before an unwell person passes through customs and out into the public.
Owing to the recent threat of Ebola, however, those checks are about to become more obvious, and more specialized, at least temporarily.
According to the NYT, five US airports will begin screening passengers arriving from West Africa with new procedures, including a contactless thermometer to test for fever, and a questionnaire to determine a person's risk.
Airport News / Airports / LAX / JFK / EWR / MIA / Tech Travel / Travel Tech / → All Tags
Plug In at Fresh Charging Stations, from JFK to LAX
On your next trip out of town keep an eye out for some charging stations at a few major airports. Sure it's not worth scoring some airfare just to check them out, but we can't complain when someone is doing something to make the travel experience that much better.
In total there's 169 brand new charging stations doing their thing, as they've been installed over the last couple weeks at New York's John F. Kennedy International Airport, New Jersey's Newark Liberty International Airport, Los Angeles International Airport, and Miami International Airport. Look for them in areas where plenty of travelers are coming and going—think spots like the gate areas, the restaurants, and the shops.
WiFi / Airport News / Airports / EWR / JFK / LGA / SWF / → All Tags
Think Fast for 30 Free Minutes of WiFi at New York City's Airports
Okay—so it's better than nothing, as we're making some concourse connectivity progress when it comes to the technology offerings at airports in and around New York City.
JFK, LaGuardia, and Newark are now flipping the switch on complimentary WiFi; however, just note that usage will be limited to 30 minutes. That's plenty of time to check-in on social media, or to update the boss to let her know that you've come down with a cold and won't be into the office—perhaps because you're actually on the way to a long weekend away.
The new deal is all part of a plan by those folks in charge of the airports—the Port Authority of New York and New Jersey. They also oversee things a little bit up the Hudson River as well, so the WiFi will also be an option at Stewart International Airport.
Travel Tech / United / EWR / Passports / → All Tags
Automated Passport Kiosks Are So Hot Right Now
Sorry to be the bearer of bad news to travelers who've shelled out for Global Entry, but a better option is quickly gaining popularity at major US airports...and it's free to use.
The automated passport kiosk allows travelers arriving from international lights to quickly enter their details, snap a photo, receive a stamp, and continue on their merry way into baggage claim; this is as opposed to waiting in a long line to see an agent for the usual passport review and stamp.
We first experienced the smooth and snappy process back in October, and fell in love with it. It seems that airports and fellow flyers echo our feelings, as over 10 airports have added the machines, with more to come:
Airport News / Airports / EWR / Newark Travel / United / AirTrain / Public Transportation / → All Tags
Why You'll Want to Avoid Newark Airport for 75 Days This Summer
Granted, our usual NYC travel advice is to avoid Newark Airport whenever possible, but take it doubly serious this summer as public transportation to/from the airport is going to be the worst.
FlyerTalk alerted travelers to the situation, confirmed by the Port Authority and local news reports.
The Newark Airport AirTrain will be closed for necessary repairs for 75 days during summer's peak travel season: May 1 - July 15. Rubbing salt into the wound, not even Amtrak will be stopping at Newark Airport's train station, meaning the only option for public transportation is a replacement shuttle bus leaving from downtown Newark.
Additionally, with no AirTrain, travelers making transfers between terminals or heading out to the parking or rental car lots will also need to pile in the shuttle buses. Yuck.
Flight Reviews / United / MUC / EWR / Budget Travel / Economy Class Travel / → All Tags
Flight Review: Cheap and Easy from Newark to Munich on United
The flight: United 106 from Newark to Munich, Germany.
We're not huge United flyers and usually prefer to hop the later Lufthansa flight out to Munich, but the price for United was too good (under $500!) to pass up in this instance.
Seated all the way in the backseat 38Kisn't bad at all for a tad over 7 hours to Europe. Heck, we've endured a less comfortable seat for non-stops to Asia, so we boarded the plane already with a sense of "this won't be bad at all." This is regular Economy, not even "Economy Plus," as United calls their premium economy option, offering a few inches more legroom for a hundred dollars or more.
Travel News / Singapore Airlines / Qantas / EWR / Airbus / A340 / SIN / DFW / SYD / 747 / Airlines / Airline News / Airplanes / → All Tags
There's a New 'Longest Flight in the World' and It's a Doozy
The king is dead, long live the king!
Just before dawn across the world at Singapore-Changi Airport, an Airbus A340-500 touched down and taxied to its gate. The passengers filed off, followed by the flight crew, and there was no fanfare for this last arrival of the longest flight in the world.
At nearly 18 hours and 9,500 miles, Singapore Airlines' SQ 21/22 between Newark and Singapore was a monster of a nonstop which only accommodated 100 travelers in all-Business Class comfort. All good things must come to an end, however, and Singapore dropped the route in order to trade in the old, gas-guzzling, four-engine A345s for some shiny and efficient A350s to come.
It's a smart move, but a sad one regardless. Singapore 21/22 will live on through the stories of those who flew it, and we're proud to add our hour-by-hour account to the aviation history books.
Moving on, another flight route must now ascend to the throne and claim the title of "longest flight in the world." For that, look no further than Texas.


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Beautiful/Decay's sister company, Something in The Universe, recently wrapped up re-vamping Los Angeles music-infuenced brand Atticus Clothing's web site! SITU "riffed" off their loose, energetic rock-music vibe throughout the site, from page layout to fonts. Check it out!
Jesse Moynihan
Philly-based Jesse Moynihan draws comics, plays guitar and violin, is a part of post-Supertramp supergroup Make A Rising. He also like jogging and jump-roping. I'm going to go ahead and imply that maybe he'd like jogging buddy if you're in the area. I love the heros / villains in his comics.
Iva Gueorguieva
Iva Gueorguieva, currently a Lux Art Institute resident, is a Bulgarian-born artist that paintings are incredibly filled with energy portrayed with color, composition and expressionist brushstrokes. The dreamlike abstract landscapes are highly energized by the amazing intensity in colors and the layers create such dynamic organic like environments, they can't help but captivate the viewers attention with the exuberant hues and dizzy brushstrokes.
Vania Zouravliov
Russian native, Vania Zouravliov's whimsical and highly detailed illustrations are exquisitely gothic and layered with symbolism. Given the high quality of both craftsmanship and detail, as well as the rigorous layers surrounding the morbid characters in the illustration, I think the more often the viewer looks at the work, we're able to discover a different aspect in the piece. In itself the process of uncovering the many hidden symbolic details brings out an even richer experience while looking at the work.
Jesse Hazelip
Awesome show from California artist Jesse Hazelip, 'Sentimental Journey' at White Walls in SF. Keep your eyes peeled if you're in the bay area because Hazelip's work is up all over the streets, so it's no surprise the show is chock full of large wheat-pastes and images on found materials. Much of the work comments on failures of our past seemingly ignored by the actions of the present, and attempts to open a dialogue about the repeating mistakes of American history. The show is up until January 30th, so go before it's too late!
Matthew Porter
'Wow' is usually the first thing I say when I look at Matthew Porter's photographs. Big, bold, and wildly imaginative, Porter fabricates iconic images straight out of a teenage boy's day-dream. All critiques aside, it's pretty cool to see a muscle car flying through the air, no? His latest show "High Lonesome" runs through January 23rd at M+B in LA, so hurry up and check it out!
Conor Harrington
Fresh amalgamation of styles from UK artist Conor Harrington. A little bit of renaissance influence mixed up with some graff inspired strokes, all executed with a masters touch… what's not to like? Find more of Conor's work at Kinsey/Desforges in LA.
Jeremy Willis
Jeremy Willis had me over to his Brooklyn studio and we hung out and talked about his paintings. Willis describes the paintings anthropomorphically – as wanting to be doing something human, like giving birth, hugging you or selling you illicit substances. The majority of the paintings are big and surround you with saturated colors and chaotic space. They do feel like they have an overwhelming emotional content, and the paintings reflect the messy nature of life. Look for more from Jeremy soon.


text:
ROBERT GUERRERO: 145 ¾ Pounds
SELCUK AYDIN: 146 ½ Pounds
SHAWN PORTER: 146 ¼ Pounds
ALFONSO GOMEZ: 146 ½ Pounds
HUGO CENTENO: 152 Pounds
AYI BRUCE: 151 ½ Pounds
GEORGE GROVES: 169 ½ Pounds
FRANCISCO SIERRA: 170 Pounds
Time Permitting:
MANUEL AVILA: 122 ½ Pounds
RAYMOND CHACON: 121 ½ Pounds
Guerrero vs. Aydin, a 12-round fight for the WBC Interim Welterweight World Championship, is presented by Golden Boy Promotions in association with Arena-Box Promotion and sponsored by Corona, DeWalt Tools and AT&T. The SHOWTIME CHAMPIONSHIP BOXING telecast will air live at 10:00 p.m. ET/PT (delayed on the West Coast) with Shawn Porter facing Alfonso Gomez for the NABO Welterweight title in the co-featured fight and preliminary fights to air live on SHOWTIME EXTREME® at 8:00 p.m. ET/PT.
Tickets priced at $252, $127, $62, and $26 are available for purchase online at Ticketmaster.com, charge by phone at (800) 745-3000 and at the HP Pavilion Ticket Office.
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text: Leiseberg Splash Pad is not open at this time. We will post when it opens. Apple Blossom Run Race Results http://racetime.info/results/2015-results/2015-05-results/5K Stroller 5K Age Group5K Overall10K Age Group10K Overall The Holiday Shopping Blitz is coming back this year! Better, bigger, and more vendor & shopper friendly in a new location: Bartlett Community Center Gymnasium! Crafters and vendors are now being accepted and limited to the first 100 entries. Don't miss this great event Friday, December 11th. For the vendor/crafter application go to www.bartlettparks.com/programs


text:
Trucks make great hot rods and this 1929 Ford Model A pickup offers all the same tricks as its roadster and coupe siblings with... More
Bob Reilly at Rosner MotorSports is privileged to offer this incredible piece of American history. Our 1934 Ford "Contractors Special" is powered by its... More
Offered here, is a very clean 1930 Model A Deluxe Rumbleseat Coupe. I am advised this car was completely restored is 2002 including the... More
1930 MODEL A SPORT COUPE SPECTACULAR RESTORATION! FORMER MUSEUM CAR! GORGEOUS COLOR COMBINATION! MANY OPTIONS, INCLUDING: RUMBLE SEAT 3-SPEED MANUAL TRANSMISSION DUAL REAR VIEW MIRROR RADIATOR MASCOT/GRILL GUARD WIND WINGS DUAL TAIL LIGHTS COWL LIGHTS WINDSHIELD... More
Clean undercarriage Refinished seats Stunning Maroon Paint Side Mount Tire Wind wings Excellent top with glass rear window with oak upper bows that match the oak bed Oak rear frame Single... More
This 1927 Ford Roadster has a 1927 Ford Roadster 350 C.I. V8 w/ 4BBL Carburetor, TH350 Automatic Transmission, 10 Bolt Rear w/ 3.07:1 Ratio... More
1928 Ford Model A1928 Ford Model A in great conditionCompletely redone both within and out (mechanically and cosmetically) Ford Model A truckThis truck is... More
1928 Ford Model A Roadster Pickup VIN A214740 This beautiful custom street rod was built in 2004 by a skilled fabricator. He owned several... More
1931 Ford Model A Fully Restored Has Jump Seat, AM/FM Stereo W/CD Player. Runs & Drives Excellent. Very Fun Car! 3700 Miles. CALL TODAY! 248-373-5811 $24,995... More
1929 Ford Model A Roadster1929 all steel Ford Roadster with 302 Ford motor. C4 auto trans 8 rear end 4 dropped front axle Disk... More
1928 Ford Model A Cabriolet If you are interested in this vehicle or have any questions, please call Dave at (541) 447-2196 All steel body, 327 bored .30... More
1929 FORD MODEL A REPLICA BUILT BY SHAY USING ALL BRAND NEW FORD PARTS, AND SOLD AT FORD DEALERS WHEN NEW. VERY AUTHENTIC TO THE... More
Presented here is a 1929 Ford Model A Rumble Seat Sport coupe that has been treated to a nice older complete restoration. The 1929... More
1930 MODEL A ROADSTER STANDARD, THORNE BROWN, RED STRIPE, BLACK FENDERS AND WHEELS. bLACK INTERIOR WITH BLACK TOP AND TAN TOP BOOT. REAL NICE... More
1930 Ford Model A Tudor Sedan (2-dr.), Very nice older restoration in burgundy and black with red pin stripe, black wire wheels and authentic... More
I have known of this Ford for years. This is the last car out of a Model A collection. Jack Vogt came to me... More
What's better than speeding around in style, and in the comfort of air conditioning? This Ford Model A pickup offers the unique feeling of... More
Of the dozens of Model A body styles, few are as universally loved as this 1931 Model A Sports Coupe. Practical yet sporty, it... More
1929 Ford Model A Tudor sedan,an excellent restoration,off the frame,completed some time ago,but still presenting very well.Looks,runs,and drives first rate. ... More
Offered for Sale by the California Automobile Museum Beautiful Restored 1929 Model A Roadster We Offer Financing Exterior Yellow and black paint with brown accents presents very well. ... More
Just arrived, a beautiful custom Model A build that is show quality. Only about 1,600 miles since completed. Finished about four years ago, it started with... More
One look at this delivery truck, and you know its real purpose now is to haul more than cargo. It's a Ford Model... More
1929 Ford Model A 2 Door Sedan If you're interested in the vehicle or have any questions, call Juan at (503) 653-4030 All original steel Briggs body. The... More
1929 Ford Model A Street Rod A box full of paperwork on the build is included in the sale. VIN AI168624 Mechanics SBC 305ci... More
1928 Ford Model A AR VIN A10832 Very Rare ''AR'' Early Production Model A has experienced a complete Concours quality, nut-and-bolt restoration that was... More
Very good running rebuilt engine with Weber carb Converted to 12-volt system Brand new white wall tires, over $800 Chrome bug catcher Wood bed is in good shape,... More
Engine: 4 Cyl Transmission: Manual Exterior Color: Blue & Black w/ Tan convertible top Interior Color: Brown & Blue Our 1930 Model A is an all steel body,... More
1931 Model A "Street Rod". All steel body, 302 V8 with automatic transmission. Roof is from a Chrysler minivan and seats are from a Fiero. This... More
Beautifully restored Model A Deluxe 2-Door Phaeton! One of only 2,229 built! Mechanically outstanding and ready to cruise anywhere! Model A collectors should not... More
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1930 FORD MODEL A 5 WINDOW COUPE DELUXE TOURING MODEL If you're interested in this vehicle or have any questions call Bill at (541)382-8038 THIS IS A VERY... More
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GR Auto Gallery is excited to present this 1930 Ford Model A Tudor for purchase. With Model T sales dwindling, the Model A was... More
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1929 Ford Model A Retro "Woody" Wagon Buick V6, 350 Automatic, Custom Front & Rear Suspension Red with Tan Custom Interior The late-1927 introduction of the Ford... More
No old car collection is complete without a Model A Ford and this 1929 Model A four-door sedan (or ''fordor'' in Ford-speak) is a... More
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Up for sale is an unrestored 31 Model A Slant Windshield. Car runs out great! They can only be original one time! Call Harold... More
1932 Ford Model A Phaeton replica-factory built "Glassic", 4 cyl. Scout chassis, engine etc. 111hp, 95mph, 3-speed, wide whites, 4 seats, dual spares, luggage... More
niagara blue with gray pin stripe body, black fenders, tan leatherette interior, black top, 5 black wire wheels, 5 new Firestone black wall tires... More
1930 Ford Model A,rumble seat roadster,a very decent,older restoration,done authentically,and holding up well.This A is ready to drive and enjoy.... More


text:
Mike: Shut the fuck up. And let me die in peace.
from Breaking Bad (Ft. Thomas Schnauz) – Say My Name on Genius
Breaking Bad uses their one allotted f-bomb from the first half of season 5 to give one of the biggest badasses on the show a proper exit. http://www.youtube.com/watch?v=m4EpX_CUr6I
To help improve the quality of the lyrics, visit Breaking Bad (Ft. Thomas Schnauz) – Say My Name and leave a suggestion at the bottom of the page


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text:
Buenafe sizzles as Ateneo bests rival La Salle anew
More News from Celest R. Flores
MANILA, Philippines — Ateneo systematically parried each of rival La Salle's runs all game for a convincing, 77-67, triumph Saturday in the 75th UAAP men's basketball tournament at the Smart Araneta Coliseum.
Ryan Buenafe regained his fierce form and exploded for a career-best 24 points — highlighted by his classic hang time moves — and eight rebounds in the Blue Eagles' eighth straight win.
Ateneo, gunning for five-straight championships and perhaps the last under head coach Norman Black, clinched a playoff for a final four berth.
"We really wanted to re-establish ourselves as the best defensive team in the league," said Black.
Buenafe, who sat out the entire last season, hit his first 20-point game since chalking up 23 markers in game two of the 2010 finals against Far Eastern University.
"I was pumped up because I was ejected last game, so I really wanted to make up for it today," said Buenafe in the post-game presser.
After going 1-of-18 beyond the arc, Buenafe suddenly became a threat by knocking down three treys.
"Coach told me to step up offensively, he gave me confidence so when I'm free, I shoot it," said Buenafe.
A 13-4 run towards the end of the third quarter keyed in the Blue Eagles' victory, as they were on point in halting the Green Archers' dangerous comeback.
Top rookie Jeron Teng was made to work hard for each of his shots but still finished with a team-best 22 points, five rebounds and two assists.
Teng made it a two-point game, 42-44, with 4:23 left in the third quarter with his first triple of the game — but Ateneo quickly retaliated and rebuilt its double-digit lead.
Almond Vosotros stepped up for LA Revilla, finishing with 20 points on four-of-eight shooting from downtown — pouring most of it in the first half, where La Salle held Ateneo to just nine points in the second quarter.
The Archers dropped to 6-4, tied with National University at fourth place.
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text:
Five Techniques for Corralling Your E-mail Inbox
by AccountingWEB on printer friendly
By Brett Owens
Like it or not, your e-mail inbox is likely the place where you spend the majority of your work time.
This would be fine except that it's unlikely e-mail time alone is the No. 1 source of value for you professionally, whether you bill hourly or not. While you and I may take pride in the fact that our average response time to e-mails is under three minutes, would our reputations be tarnished with a slightly longer response time?
Many time management and productivity sages recommend the noble goal of checking e-mail only once or twice a day – but many, me included, find it difficult to put that into practice in the "real world."
So, what are some practical steps we can take to improve our handling of e-mail; i.e., spend less time in our inboxes and more time on productive, value-added activities? Here are five best practices for Microsoft Outlook (and Gmail) that have worked well for me.
1. Keep your inbox as empty as possible
Either delete or file e-mails as soon as you open them. If they sit in your inbox they will drive you crazy. You'll end up looking at each e-mail over and over again, thinking how you "should" get around to addressing that item. Then, if you procrastinate, it'll be the same drill next time you look at your inbox – you'll look at the same e-mail and decide whether to act or not.
Do yourself a favor: if you decide not to act immediately, file it away in a folder. Then, at a later time, you can batch process all items in that folder.
By doing this, you'll build more momentum as you do your follow-up e-mails, because you'll be tackling items and issues that are related. This will go a long way toward preserving your sanity, because having an unofficial to-do list stacked up in your inbox creates a low level of stress that's always eating away at your sanity.
2. Use "Rules" to automatically file e-mails
I used to let every single e-mail drop directly into my inbox. What a nightmare that was. Looking back, it was a crazy practice. All of my correspondence, newsletter subscriptions, etc., came right into the same place. Yikes!
If you don't yet use Outlook's Rules and Alerts, you can find the feature under Tools > Rules and Alerts. I find the Stay Organized rules to be the most useful, where I create a rule to move an incoming message to a predetermined folder, based on the sender or subject line.
This is a great way to manage newsletter subscriptions, as it allows you to read related messages in batches, which can be a big help in managing your workflow.
(Note: The analogous feature in Gmail is Filters. You can find them under More Actions > Filter Messages Like These when you have a message open.)
3. Dial down the send/receive frequency
Under Options > Mail Setup > Send/Receive, you can control the frequency in which Outlook checks for new mail. And believe me, the longer the duration you set this option, the better off you'll be.
I used to have this set at a cool two minutes. And what happened? As soon as an e-mail came in, I was on it! Well, great, but is an immediate reply really needed? Would it have killed me not to know about the e-mail for an extra ten, thirty, or even sixty minutes? Of course not.
So I now have it set at a respectable thirty minutes. If I don't cheat by manually hitting the Send/Receive button myself, I can actually focus on a task for thirty minutes, before the bright, shiny object that's incoming mail distracts me.
A setting of sixty minutes or longer would probably be even more effective, but hey, I'm still a recovering e-mail addict – it's all about baby steps.
Remember, even by setting this to sixty minutes, your response time to e-mails will be roughly within the hour. That's still pretty awesome and will be a lot less wear and tear on you than the instantaneous response time many shoot for.
And, you can get A LOT done when you work interrupted for just thirty or sixty minutes at a time. The problem is we rarely do!
4. Close Outlook Completely
If you really have to get serious about getting work done, how about closing down Outlook? This is an advanced corollary of tip 3.
Sometimes this can be quite scary. Will the world end? What e-mails might come in while you're actually accomplishing something productive?
I have to admit I haven't yet worked up to this level during working hours, but I've found it a good thing to close Outlook (and/or Gmail) for extended periods at night and on weekends. Mobile devices can really help here, as they allow you to casually monitor incoming e-mails, while making it inconvenient to actually compose one.
5. Send fewer e-mails, especially during off-hours
The familiar refrain "it's better to give than receive" doesn't apply to e-mails, where the more you give, the more you'll receive.
So not only should you focus on sending fewer e-mails overall, but you should be especially diligent about keeping your e-mail habit on a "need-to-send" basis during off-hours and weekends. Again, a mobile device diet may provide the sufficient discouragement you need.
Since we all check e-mail 24/7 these days, thanks to the magic of smartphones, you've probably received e-mails from fellow workaholics during unconventional working hours. Don't get suckered into their game of looking productive.
E-mail volleying, like little kids antagonizing each other, is a two-way street. And remember, it only takes one grown-up to stop the madness!
Related articles:
- Three Irreverent Time Management Strategies for the Digital Era
- It's about Time: Successfully Managing a To-do List
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There's always more at AccountingWEB. We're an active community of financial professionals and journalists who strive to bring you valuable content every day. If you'd like, let us know your interests and we'll send you a few articles every week either in taxation, practice excellence, or just our most popular stories from that week. It's free to sign up and to be a part of our community.
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Editor's Choice
As part of our continued effort to provide valuable resources and insight to our subscribers, we're conducting this brief survey to learn more about your personal experiences in the accounting profession. We will be giving away five $50 Amazon gift cards, and a $250 Amazon gift card to one lucky participant.
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text:
Overall: 5.0 / 5 based on 3 reviews
3 of 3 reviewers would recommend this product to a friend.
By: CCSC
I love this shirt! -
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I recently purchased the aqua women's drirelease short sleeve T shirt, and I love it. It's is nice and cool on hot days, and it dries very quickly. Since it's odor resistant, it doesn't get smelly when I work out in the yard all day either. I especially like the color and the styling with the slightly contrasting stitching. It will be one of my go-to shirts to take on vacations during the summer months since the wrinkles fall right out of it, and it could be washed and hung to dry overnight.
Great purchase -
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Good fit, nice style, and very comfortable. Affords full range, easy movement during extensive, hot weather activities.


text:
STARmeterSEE RANK
Up 4,373 this week
Caroline Flack
Caroline Flack was born on November 9, 1979 in London, England as Caroline Louise Flack. She is known for her work on The Xtra Factor (2004), Gladiators (2008) and I'm a Celebrity, Get Me Out of Here! NOW! (2002). See full bio »
Born:Caroline Louise Flack
in London, England, UK
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Represent Caroline Flack? Add or change photos


text:
Nearly 400 posts linked up ... WOW.
Even though this party was all about celebrating Jaime, my buddy at Crafty Scrappy Happy -- I thought I'd feature some of MY very own favorites, too!
(Hope you don't mind!)
Good stuff. I found so many great blogs from this party! Like I needed more to read:). Have a great weekend!ReplyDelete
Love all your Fun picks... I don't think I will ever look at plastic lids the same EVER again... :) Thank you so much for the feature of Mrs. Pearl.. She would LOVE to have a friend so be sure to let me know when you get one... Maybe they can be pen pals... :)ReplyDelete
Oh I just signed up to follow you Via Linky... :)ReplyDelete
Thank you so much for the awesome feature! I just love your blog! So much creative inspiration.
Yay! Thanks for including my stools in your features! What an awesome linky party that was.ReplyDelete
:o) I had so much for seeing what you picked as your favorites! It was SO hard to choose! I am SO glad you were part of the fun!!!
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$222.50 24 bids <1m
are bidding on KATO 106-016 & 106-026 BALTIMORE & OHIO SMOOTH SIDE PASSENGER CARS IN SCALE N .TOTAL 10 CARS ( TWO SETS)THESE ARE STOCK FROM A CLOSED SHOP ESTATE. Item is new, and never
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Shipping and Handling within 24 TO 48 hours after payment.
For shipping outside USA; please let me know before bidding.
Import duties, taxes and charges are not included in the item price or shipping charges. These charges are the buyer's responsibility. Please check with your country's customs office to determine what these additional costs will be prior to bidding or buying. These charges are typically collected by the delivering freight (shipping) company or when you pick the item up.
© 2008-2015 PicClick LLC.


text:
June 6, 2011
At the Space Shuttle Discovery's Thermal Imaging Printing System (TIPS) station, astronaut Curtis L. Brown, Jr., mission commander, checks some 50 pages of data sent up by flight controllers. On Brown's left wrist is a band associated with a Detailed Supplementary Objective (DSO). A crew mate's socked feet appear in the upper right corner.
Topics: Space Shuttle, Human spaceflight, Space Shuttle program, Curtis L. Brown , Jr., Edwards Air Force Base, Spaceflight


text:
Updated from 5:09 p.m. EST to provide comments from the conference call and updated share price.
CUPERTINO, Calif. ( TheStreet) -- Apple (AAPL - Get Report) blew away Wall Street estimates, and revenue moved well past the $40 billion mark in its latest quarter, as sales of the iPhone, iPad, and Macs were extremely strong for the holiday period.
Apple reported earnings of $13.06 billion, or $13.87 a share, on revenue of $46.33 billion for its fiscal first quarter ended Dec. 31. Analysts polled by Thomson Reuters expected a profit of $10.15 per share on just over $39 billion in revenue in the period.


text:
BEIRUT: A leading rebel commander has met with Druze sheikhs in the northern province of Idlib and received praise for his "moderation."
The sheikhs met with Jamal Maarouf, who heads the Syria Rebels Front, which is affiliated with the Free Syrian Army.
In a video posted this week of the encounter, Maarouf is seen talking to a group of sheikhs in a room, as he says that anyone espousing sectarian or other types of "division" is unwelcome within rebel ranks.
"No matter what his [ethnic or sectarian] affiliation is, any Syrian citizen … and the Syrian people – they're all my brethren," he said.
"This is the first time we've heard such talk," one of the sheikhs responded. "We've seen extremism, as you know," the sheikh continued.
"When someone speaks moderately, and is concerned with the nation, then anyone who is sane will support him."
Idlib is home to more than a dozen Druze villages, whose residents have largely been spared direct involvement in the 3-year-old civil war. Maarouf is also from the province.
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But many parents were unaware of the lockdown and of a manhunt launched by authorities. Text and email alerts were sent, but most parents of the district's 5,000 students were not signed up to receive them.
But many parents were unaware of the lockdown and of a manhunt launched by authorities. Text and email alerts were sent, but most parents of the district's 5,000 students were not signed up to receive them.
The Jordan School District is expecting enrollment to increase by more than 29,000 students in the next 10 years for the communities it serves, which include Herriman, South Jordan, Riverton, Bluffdale and West Jordan.
There are 21 layoffs recommended for the board of education's approval at its meeting at 6:30 p.m. tonight at the Sarvis Conference Center, 1231 E. Kearsley St.
The program's website lists several areas in which preschool children are assessed, including language and mathematical development, physical skills and social interaction.
If you've been paying attention to the national news lately, you've certainly caught wind of the catastrophic budget problems that the Philadelphia public school system is currently facing. This isn't only happening in Pennsylvania, but other areas across the country that view budget cuts as an option to "fix" public schools.
Boys are diagnosed with learning disorders and attention problems at nearly four times the rate of girls. They do less homework and get a greater proportion of the low grades.
SB47, from Sen. John Coghill, relates to boarding schools. It increases the per-pupil monthly stipend to cover room and board expenses and expands to schools that started after 2005 eligibility for the stipend program.
Superintendent Greg Gray said $30 million worth of work is scheduled to be completed this summer. It's part of an $88.4 million bond issue voters approved last year.
The Ranch Academy's Superintendent, Melissa Pardue, needed a customizable observation solution to meet the unique needs of their campuses in Tyler and Canton Texas where teacher support and collaboration are top priorities.
In a last-minute set of changes to the state budget, Assembly Republican leaders plan to boost school voucher programs, remove a proposed cap on a property tax credit for disabled veterans and allow new rules to keep protesters away from the site of a proposed mine.
Schools are using digital signs more widely to convey information to students, faculty, and visitors. From emergency alerts and interactive event schedules to serving as backdrops for marching bands, digital signs are making communication more attractive and efficient.
Now that students have the answer to everything they could ever want to know in the palm of their hands, at the swipe of the finger, or at the click of a mouse, "what" they know is becoming less important every day. Today, it's more important to teach students how to effectively use the technology and resources at hand to solve real-world problems.
The National Museum of Mathematics (MoMath) is joining bookstores and libraries in more than 50 locations around the country for the first annual Bedtime Math National Pajama Party Week, aimed at making math fun, exciting and relevant for children three to eight-years-old.
Parents in the Polk County (Fla.) district received a letter stating a pilot program would track bus riders using each child's iris. Parents could opt out, but by the time they learned about the program, the company charged with gathering the information had already captured images of about 750 children's eyes.
Open content, electronic textbooks, personalized learning, cloud technology, and learning analytics are emerging technologies that K12 administrators will integrate into schools over the next few years, according to the New Media Consortium's Horizon Report on tech trends.


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Follow FEMA:
Deputy Administrator and DHS Secretary visit a Local Shelter
Main Content
Photo by Jocelyn Augustino - Nov 01, 2012
Staten Island, N.Y., Nov. 2, 2012 -- FEMA Deputy Administrator Rich Serino, left, and Department of Homeland Security Secretary Janet Napolitano, speak to local residents at a shelter set up at Susan Wagner high school. The shelter is set up to assist residents impacted by Hurricane Sandy. Jocelyn Augustino/FEMA - Location: Staten Island, NY


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This bright and airy yoga center has classes in several forms, such as Vinyasa and Anusara, as well as Pilates, massage and acupuncture. The laid-back studio, which has another location on 35th Ave, hosts a lounge area and store, where such goods as yoga mats, bags, rugs, straps, and aromatherapy balms and sprays are on offer.
The Yoga Room Long Island City
|Venue name:
|The Yoga Room Long Island City
10-14 47th Rd
Long Island City
|Cross street:
|between Vernon Blvd and 11th St
|Opening hours:
|Schedule varies, check website for listings.
|Subway: 7 to Vernon Blvd–Jackson Ave
|Prices range from $18 for a single class to $160 for 1 month unlimited.