text:
Gender: Female
Usage: Zoe is a very popular first name. It is more often used as a girl (female) name.
People having the name Zoe are in general originating from Australia, Greece, United Kingdom, United States of America.
For another variant of the name Zoe across the world, see Zoé, Zoë.
- Life
If you have more information, please share it in the comments area below.
The Growth number corresponding to this first name is 1.
The name Zoe is ranked on the 677th position of the most used names. It means that this name is very frequently used.
We estimate that there are at least 837700 persons in the world having this name which is around 0.012% of the population.
The name Zoe has three characters. It means that it is relatively short-length, compared to the other names in our database.
The graph below represents the number of people who were given the name Zoe for each year since 1900 in the U.S.A.:
We do not have a name day for Zoe.
For other names check our Name Day Calendar
History and Origin
Zoe (and variants) is a female first name.
English: from the Greek name meaning 'life'. This was already in use in Rome towards the end of the classical period (at first as an affectionate nickname), and was popular with the early Christians, who bestowed it with reference to their hopes of eternal life. It was borne by martyrs of the 2nd and 3rd centuries, but was taken up as an English given name only in the 19th century.
Variant: Zoe.
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text:
Yekra Player
Yekra is a revolutionary new distribution network for feature films.
Harold Camping sounded the alarm. May 21, 2011 was to be Judgment Day, God's intervention into our worldly affairs. The Bible guaranteed it! For most Americans, these are the Last Days. Fully 79% of Americans believe Jesus will return to earth someday. Forty percent believe Jesus will return before 2050! Apocalypse Later traces the roots of end times proclamations back to the Book of Daniel, on to the historical Jesus, and lastly Paul, the greatest of the Apostles. The same excitement that Harold tapped into for his May 21st prediction is the same excitement that helped birth a world religion 2000 years ago.

subset: commoncrawl


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Rachel Tote
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- Large tote
- Watersnake
- Two top handles
- Magnetic closure
- Canvas interior with zip pocket and a removable snakeskin pouch attached with hidden magnetic closures
- Removable pouch has slip pocket at back and flap opening with snap closure
- Body length 17 inches; height 12 inches; width 4 inches
- Drop handles 10½ inches
- Pouch: body length 8½ inches; height 4¼ inches; width½ inch
Material: Watersnake
Brand: Beirn
Origin: Imported


text:
Pronunciation: kalvosin
Added on: 2010-12-25 Listened: 5 timesin: clothing, clothing in Finnish
Pronunciation in Finnish [fi]
- Pronunciation by JoyJoy (Female from Finland)
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22 March 2013 13:15 [Source: ICIS news]
TORONTO (ICIS)--?xml:namespace>
In its budget announcement late Thursday, the federal government said that it will grant a two-year extension to accelerated capital cost allowance (ACCA) for new machinery and equipment, and that it will provide funding to encourage innovation in manufacturing and address skills shortages.
The ACCA, first introduced in 2007, was due to expire by the end of 2013. It allows manufacturers to defer the taxes they pay at the beginning of a project - when cash flow is most urgently needed - until a later date.
"Measures like the ACCA for new manufacturing machinery and equipment can make the difference between a company investing in
CIAC also lauded the government's decision to provide funding to encourage innovation and improve the competitiveness of
Furthermore, the government's move to address skills and training – in partnership with provinces and employers – was also important for the chemical industry, as it currently employs an aging workforce and faces skills shortages, the group said.
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text:
3 - Pc. Magnetic Tool Accessory Set
Item # WX2-230179
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- Details & Specs
3 - Pc. Magnetic Tool Accessory Set gets a grip to keep things organized! SAVE BIG!
For your kitchen, shop, grill, garage... 2 Magnetic Tool Bars and a Paper Towel Holder that hold on magnetically tight. Just stick the magnetic back to any ferrous (that's the fancy word for iron / steel) surface.
The 12" Magnetic Tool Bars can hold wrenches, screwdrivers, sockets, fasteners, knives, cooking utensils and so on. The Paper Towel Holder... well, that kind of speaks for itself. Each weighs approx. 16 ozs.
The ideal flexible organizers to keep things neat and handy... get yours ONLINE today and save!
- Reviews
3 - Pc. Magnetic Tool Accessory Set is rated out of 5 by 1.Rated 3 out of 5 by GRIZ/LINDA M from the set as a whole is of good quality other than the noted isses below. Wish the descriptions were a bit clearer on the issue of mounting anywhere, I thought them to be "MAGNETIC" not screw on.Date published: 2014-05-15
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text: Idea Exchange: Label instruments with engraved pet tags Sep 01, 2011 By dvm360.com staff VETERINARY MEDICINE We used to have difficulty labeling our endoscopy instruments because the labels would wash off every time we disinfected the instruments. We decided to have engraved pet tags made for each instrument and attach them with a key ring. We also color-coded the tags based on which endoscope they work with. Now we can easily find the instrument we need. Kelly Pratt, RVTAtascadero, Calif. Related linksRead more Idea Exchange tipsIdea Exchange: Tag traveling petsIdea Exchange: Velcro stops jingling tags Hot topics on dvm360Vetcetera: DVMs' dirty little secrets, hidden pain and path to happiness Vetcetera: APOP says more than half of U.S. pets are overweight 4 core skills to help you deliver bad news to veterinary clients A veterinarian's view on exercising with pets Michigan state veterinary board rules against Dr. Pol


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Have you been attempting to get your hands on information about Humberto Moya in Orlando, FL? If so, you just got very lucky because USA People Search is amazing at providing people search data such as address, public records, and possible relatives for people like Humberto Moya. Also, with so much data about people with the last name Moya in Orlando in our records, you are sure to find the specific person you are searching for.
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If your child makes plans and starts thinking about Transition early it will be easier. The Welsh Government leaflet 'My Life, My Way - A Young Persons Guide to Transition' will help them find out what should happen and when(please see Document Links on the right hand side of this page).
Further information for young people moving into adulthood can also be found on the Directgov website. The website highlights the major life changes that may be experienced as a disabled teenager - including choices in education, health and independent living.
What is Transition Planning?
Transition Planning starts for your child in Year 9 (aged 14 years). Individuals working with your child will meet to share information, this information will be recorded and a Transition Plan will be constructed by the young person's respective school. The Transition Plan when they are first drawn up is not simply about what is available when leaving school, but can include plans for ongoing school provision.
The Transition Plans should also include:
The Transition Process commences with the first Transition meeting which takes place when your son/daughter reached the age of 14. The Transition meeting will be organised by your son/daughter's school and as parent(s)/carer(s), you will be invited to the meeting on equal terms with professional staff to ensure that your views and wishes are recorded. Transition Planning/Reviews will continue to take place until your son/daughter will leave school.
People who may be invited to the Transition Review Meeting include:
A Parent(s)/Carer(s) Guide to Transition and Transition Planning for Young People is available (please see Document Links on the right hand side of this page) or for further information please contact the Transitional Development Officer/Senior Social Worker.


text:
Pumpkin Head Men's Yellow T-Shirt
Inspired by Saw
(approx. $15.98 or €11.79)
- Product Type: Mens T-shirt
- Ships in 1-2 days
- UK & Worldwide Delivery
If you love mind games then this epic tee is a must have! Inspired by the horror fuelled film series 'Saw', this halloween design is a great way to show off your love for the twisted stories and scare passers by on October 31st!


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Group I introns are one of the four major classes of introns as defined by their distinct splicing mechanisms. Because they catalyze their own removal from precursor transcripts, group I introns are referred to as autocatalytic introns. Group I introns are common in fungal and protist nuclear ribosomal RNA genes and in organellar genomes. In contrast, they are rare in all other organisms and genomes, including bacteria.
Here we report five group I introns, each containing a LAGLIDADG homing endonuclease gene (HEG), in large subunit (LSU) rRNA genes of cyanobacteria. Three of the introns are located in the LSU gene of Synechococcus sp. C9, and the other two are in the LSU gene of Synechococcus lividus strain C1. Phylogenetic analyses show that these introns and their HEGs are closely related to introns and HEGs located at homologous insertion sites in organellar and bacterial rDNA genes. We also present a compilation of group I introns with homing endonuclease genes in bacteria.
We have discovered multiple HEG-containing group I introns in a single bacterial gene. To our knowledge, these are the first cases of multiple group I introns in the same bacterial gene (multiple group I introns have been reported in at least one phage gene and one prophage gene). The HEGs each contain one copy of the LAGLIDADG motif and presumably function as homodimers. Phylogenetic analysis, in conjunction with their patchy taxonomic distribution, suggests that these intron-HEG elements have been transferred horizontally among organelles and bacteria. However, the mode of transfer and the nature of the biological connections among the intron-containing organisms are unknown.
Group I introns are distinguished by a conserved secondary structure fold of approximately ten paired elements and the ability to catalyze a two-step splicing reaction in which the intron RNA is removed from the precursor RNA transcript [1]. Because of their ability to self-splice, group I (and group II) introns are referred to as autocatalytic RNAs. The majority of group I introns are found in nuclear rRNA genes and in the plastid and/or mitochondrial genomes of fungi and protists [2]. A smaller number of these intervening sequences are found in phage, viral, and bacterial genomes. In bacteria, group I introns interrupt four different tRNA genes [2], the recA and nrdE genes of Bacillus anthracis [3-6], the tmRNA gene of Clostridium botulinum [7], the thyA gene of Bacillus mojavensis [8], the RIR gene of Nostoc punctiforme [9], and the large subunit (LSU) rRNA genes of Coxiella burnetii [10], Simkania negevensis [11], several closely related Thermotoga species [12], and the cyanobacterium Thermosynechoccus elongatus (strain BP-1, formerly referred to as 'Synechococcus elongatus') [13]. Group I introns have not yet been found in archaea.
In eukaryotes, group I introns are common in protists except the excavates [14]. These sequences are particularly abundant in fungi, algae, and true slime molds. The widespread, but highly biased distribution of group I introns (i.e., frequent in some taxa such as fungi, but absent from others) suggests they have been transferred horizontally among taxa, and come to reside in different genes. Interestingly, group I introns are sometimes associated with homing endonuclease genes (HEGs) that can invade group I introns to promote efficient spread of the intron/HEG into homologous intron-less alleles [homing, reviewed in [15]]. Briefly, the HEG is expressed and intron/HEG mobility is initiated when the site-specific homing endonuclease (HE) generates a double-stranded DNA break at or near the site of insertion in an intron-less allele, soon after mating between intron-containing and intron-lacking organisms [e.g., [16,17]]. HEGs that are associated with group I introns are categorized into five families by the presence of conserved sequence motifs (LAGLIDADG, His-Cys box, GIY-YIG, HNH and PD-(D/E)XK [18,19]) in the HE proteins.
It is currently believed that most intron/HEG elements follow a recurrent gain and loss life-cycle [20]. In this model, a mobile intron/HEG invades by homing an intron-minus population until it becomes fixed at a single genic site. After fixation, the HEG degenerates and is lost because it no longer confers a biological function. Without the HEG, the intron is lost. Once the population is intron-minus the same intron/HEG element (from another population) may re-invade the same genic site. However, the evolutionary outcome may be different if the HEG or the intron gains a function other than endonuclease or splicing activity, respectively. In a few cases, intron-encoded proteins with dual roles have been reported. For example, in addition to functioning as homing endonucleases, I-TevI, encoded within the td intron of phage T4 acts as a transcriptional autorepressor [21], and I-AniI, a LAGLIDADG HEG encoded within a group I intron interrupting the apocytochrome b gene of Aspergillus, function as a maturase [22]. By gaining new biological roles the HEG and/or the intron can avoid becoming redundant and lost [see [23]].
Here we report multiple group I introns in rRNA genes of cyanobacterial strains assigned to the genus Synechococcus. A common feature of these introns is the presence of LAGLIDADG homing endonuclease genes in peripheral stem-loop regions of the group I ribozyme. To our knowledge, this is the first discovery of multiple group I introns in a single chromosomal gene of a bacterium (multiple group I introns are also present in at least one phage gene [24] and one prophage gene [25]). We analyze the structure of these newly discovered introns and investigate their phylogenetic history in the context of related introns from bacteria and organelles. In addition, we present a compilation of known group I introns in bacterial or phage genomes that encode HEGs.
Results and discussion
Group I introns with LAGLIDADG HEGs in the LSU rDNA genes of Synechococcus strains
In an unpublished study on cyanobacterial phylogeny, we sequenced the LSU rRNA gene from 25 diverse cyanobacteria. To our surprise, we found introns in two of the LSU genes, from Synechococcus lividus strain C1 and Synechococcus sp. C9, both originally isolated from a hot spring habitat in Yellowstone National Park, Wyoming, USA [[26]; see also Table 1]. The LSU rRNA gene of Synechococcus sp. C9 contains three group I introns, located at positions L1917, L1931, and L2593 (by convention, the numbering reflects the Escherichia coli genic position), whereas the S. lividus strain C1 LSU rRNA gene contains similar introns at the L1931 and L2593 positions. All five introns possess a full-length HEG, each containing a single copy of the LAGLIDADG motif. Very few introns have been reported in rRNA genes from other bacterial phyla and this is only the second report of introns in cyanobacterial rRNA genes. The first was for a single group I intron (also with a LAGLIDADG HEG) in the thermophilic cyanobacterium Thermosynechococcus elongatus [[13]; Table 1].
Table 1. Group I introns in bacteria and phage that encode homing endonuclease genes (HEGs)
The inferred secondary structures of the intronic RNAs are presented for one each of the L1917, L1931, and L2593 Synechococcus introns (Fig. 1). Unusual features include open reading frames (ORFs) that extend from peripheral loops into the intron core structure. For example, the L1917 ORF starts in P6 and continues through the group I ribozyme elements P7, P3 and P8 before it stops in P9. The double role of the ORF and ribozyme core regions suggests that these nucleotides must be under strong selective pressure to maintain the catalytic RNA functions and to preserve the genetic code for a functional homing endonuclease. Although uncommon, similar features have been noted in other intron-HEG elements [e.g., [11,27-29]]. It is also noteworthy that the L1917 and L1931 introns are very similar to subgroup IC1 introns that contain a complex P5 region and a classical group IC1 intron P7, but lack a P2 element, which often is associated with long-range tertiary interactions (i.e., with P13 and P14). The L2593 intron has a short P5 region, but contains a relatively large (ca. 65 nt) extension in the P7 region (P7.1 and P7.2) and a short P2. The P7.1 and P7.2 structures were also identified in the crystal structure of a group I intron from the bacteriophage Twort, where it was shown that they are part of peripheral structures that encircle and stabilize the guanosine-binding pocket [30]. Introns lacking the P2 element are common in organelles, and typically belong to the IC2, IA1 or IB4 subclasses of group I introns.
Figure 1. Putative secondary structure of rDNA group I introns in Synechococcus. The group I introns are inserted after positions L1917, L1931, and L2593 of the large subunit ribosomal RNA gene. Open reading frames (ORFs) that encode putative homing endonucleases (HEs) with a single copy of the LAGLIDADG motif are inserted into peripheral regions. Paired elements (P1–P10) and every 10th nucleotide position in the introns are indicated on the structures. The L1931 and L2593 introns shown are from S. lividus strain C1, whereas the L1917 intron is from Synechococcus sp. C9.
Compilation of group I introns with HEGs in bacteria and phage
At last count (2005) [see [14,31]], approximately 3% of nuclear group I introns contained a HEG. There are no systematic counts for organellar introns, but in May 2007 the intron database of ref. 2 contained 117 and 83 introns in rRNA and protein genes, respectively, of mitochondria. Of these, 79 contain an HEG, and for 49 introns the presence of ORFs was not determined. In plastids, 105 introns interrupt rDNA genes and 8 interrupt protein genes (note that there are 242 entries of the same trnL intron, and none of these contain an ORF). Of these, 11 contain an ORF and for 80 the presence of an ORF was not determined. Many of the "undetermined" entries do contain ORFs [32], but the exact number remains unclear. In summary, we estimate that at least 50 percent of organellar introns contain ORFs (this value will likely change as more sequence data are added to GenBank).
To assess the frequency of HEGs in bacteria and phage, we searched the literature to determine the total number of published group I introns with HEGs in their genomes. The results of this analysis are summarized in Table 1 and show that the majority of HEGs in bacterial chromosomes belong to the LAGLIDADG family and are found in group I introns located in LSU rRNA genes. Two members of the GIY-YIG family are found, in the chromosomal thyA gene (encoding thymidylate synthase) of Bacillus mojavensis and in the nrdE gene of a prophage of Bacillus anthracis and other Bacillus species [see Table S2 in ref. [4]]. One catalytically active homing endonuclease (I-Ssp6803I), encoded by a group I intron that interrupts the tRNA-fMet gene in the cyanobacterium Synechocystis sp. PCC 6803 [28], was recently identified as the first representative of the PD-(D/E)XK family of homing endonucleases [19]. The total number of known group I introns in chromosomal DNA of bacteria (i.e., regardless of whether or not the intron contains an HEG) is currently around 35 if homologous introns in strains of the same species are regarded as one entry (note that about 95 introns are listed at the Comparative RNA web site [2], and that many of these are multiple entries of the same intron in the same species, but in different strains). Therefore, more than 1/3 (14 of 35) of known group I introns in bacteria contain HEGs. Finally, the 14 phage HEGs belong exclusively to the GIY-YIG or HNH families. The three GIY-YIG HEGs are found in Escherichia coli phage T4 and in Bacillus subtilis phage β22, whereas the eleven HNH HEGs are found in a wide variety of phage. Our study did not involve comprehensive searches of genome databases, but is rather a compilation of known group I introns and HEGs in bacteria. For example, in a recent paper [33] many HNH HEG-like sequences were identified in bacterial and phage genomes, but how many of these are associated with group I introns is unclear. It is likely that more intron/HEG elements remain to be identified in GenBank. His-Cys box HEGs are found exclusively in nuclear introns and are not included in our compilation.
Phylogenetic analysis of HEG-containing group I introns in bacterial rRNA genes
The two unicellular, thermophilic cyanobacterial strains, Synechococcus lividus strain C1 and Synechococcus sp. C9, are distant relatives based on phylogenetic analyses of small [34] and large subunit rRNA sequences (our unpublished data). We added all five Synechococcus intron DNA and HE protein sequences to previously published sequence alignments that contain homologous LSU intron/HEs [32] and inferred phylogenetic relationships among the sequences in these two alignments. HEGs and introns that are inserted at the same rDNA positions are, in general, most closely related to one another [12,32]. Our inferred phylogenetic trees indicate that the Synechococcus introns and their HEGs form a cluster with all other known introns or HEs from the same rDNA insertion sites (Fig. 2).
Figure 2. Phylogenetic relationships of rDNA group I introns (A) and their LAGLIDADG HE proteins (B). (A) The 50% majority-rule consensus tree inferred using Bayesian analysis under the GTR + I + Γ substitution model. The tree includes only those LAGLIDADG HEG-containing group I introns that are inserted at the same four rDNA positions (Table 1) at which introns are found in bacteria. The tree is arbitrarily rooted on the branch leading to the L1917 introns. The thick branches denote ≥ 0.95 posterior probability for groups to the right of the values. Numbers above branches indicate minimum evolution (Jukes-Cantor model) bootstrap (BS) values from 2000 replicates, and numbers below branches indicate maximum parsimony values from 200 replicates. Bootstrap support values < 50% are not shown. Vertical bars on the right of the tree mark groups that share insertion positions in the LSU rDNA. Bacterial introns are in blue, chloroplast introns are in green (these are all from green algae), and mitochondrial introns are in vermillion (these are all from green algae, except for the three introns from the amoeba Acanthamoeba). Taxa labeled with an asterisk possess the novel introns presented in this paper. The scale bar indicates the inferred number of substitutions per site. (B) Minimum evolution phylogenetic tree of the HE proteins, analyzed under the WAG + Γ substitution model. The tree is arbitrarily rooted on the branch leading to the L1917 HEs. Numbers above the branches indicate the bootstrap support value (from 500 replicates) from a neighbor-joining analysis using the JTT substitution model. Other features of labeling are as in A.
Each of the four, rDNA-positionally-distinct clades of introns/HEGs contains a broad mixture of sequences from bacteria, chloroplasts (entirely from green algae), and mitochondria (mostly from green algae, but with three introns/positions from the amoeba Acanthamoeba castellanii) (Fig. 2). These patterns and, crucially, the very restricted and sporadic phylogenetic distribution of these introns (especially so within bacteria and mitochondria, less so within green algal chloroplasts) are consistent with the hypothesis that these introns have been frequently transferred horizontally among and within organelles and bacteria. At the same time, however, because phylogenetic resolution is generally poorly supported within each intron clade (Fig. 2), it is unclear as to how many horizontal transfer events may have been involved in the history of the analyzed introns, much less which clades might have served as donors and/or recipients in any particular horizontal transfer event. Greatly increasing the sampling of these intron families should help address these issues. However, the short length and therefore limited information content of the introns and HEGs will perhaps provide severe constraints on our ability to ever recover a robustly supported phylogenetic history of these mobile genetic elements.
Against this hazy backdrop of likely extensive, but poorly resolved, horizontal transfer it is possible to identify a few lineages of introns/HEs where an element seems to have been transmitted by standard vertical descent once acquired by putative horizontal transfer. Most relevant to this study, the S. lividus strain C1 L2593 intron and HE are sister to the Thermosynechococcus elongatus L2593 intron/HE, whereas the L2593 intron and HE from Synechococcus sp. C9 are sister to this pair of sequences. This evolutionary relationship is in agreement with the inferred rDNA phylogeny [see [26] and [34]; our unpublished data], and therefore also with inferred organismal phylogeny. This finding is consistent with the hypothesis that this intron was acquired only once among cyanobacteria and was subsequently subject to strictly vertical transmission. The well-supported sister-group relationship of the L1931 intron and HE from S. lividus strain C1 and Synechococcus sp. C9 is also in accord with the hypothesis of vertical transmission within cyanobacteria following initial acquisition of the intron via horizontal transfer. In both cases, however, sampling of many additional cyanobacteria, especially those likely to belong to the intron-containing "clades", is needed to better assess the evolutionary history of these introns. Nesbø and Doolittle [12] have likewise concluded that following its putative acquisition from an organellar source, the L1931 intron was subject to strictly vertical descent within a clade of nine intron-containing species and strains of Thermotoga (three of which were included in this study; Fig. 2). Finally, the well supported (Fig. 2B) pairing of L1931 HEs from plastid genomes of two chlamydomonads is also consistent with vertical intron descent in this lineage.
Distribution of single-motif LAGLIDADG HEGs
Group I introns with single-motif LAGLIDADG HEGs are found in biogeographically and phylogenetically distantly related organisms. For example, L1931 introns with single-motif, relatively conserved (Fig. 2B) HEGs are present in 1) Simkania negevensis found as a contaminant in a cell culture in Israel [35], 2) the thermophilic bacterium Thermotoga neapolitana from submarine hot springs in the Bay of Naples, Italy [36], 3) Thermotoga naphthophila from the Kubiki oil reservoir in Japan [37], 4) the cyanobacterium Synechococcus spp. from a hot spring habitat in Yellowstone National Park, USA [26], 5) mitochondrial and chloroplast genomes of a diverse array of green algae, and 6) the mitochondrial genome of the amoeba Acanthamoeba castellanii. Yet the biological connections (if any) among these organisms and the mode of group I intron transmission remain unclear. Simkania negevensis is capable of growing and persisting in acanthamoebal cells [38], indicating a potential association between these two organisms that harbor L1931 introns.
Intron/HEGs are relatively widespread but very sporadically distributed in eukaryotes and prokaryotes. According to the cyclic model for gain and loss of this type of selfish intron [20], the intron/HEG is destined for degradation and loss after a population has been fixed for the intron. However, the intron/HEG can continue to persist by repeatedly spreading into new populations or species via horizontal transfer. The enormous number of prokaryotes on our planet (estimated at 4–6 × 1030 cells [39]) and their presence in virtually every environment compatible with life may provide a constant source of intron-less populations that the intron/HEGs can potentially invade.
Given high rates of horizontal transfer in prokaryotes [e.g., [40,41]], it is surprising that only a small number of introns have been found in their rDNA genes. As of 28 December 2006, 428 prokaryote genomes have been sequenced and another 683 are in progress [42]. In addition, a search of the GenBank nucleotide sequence database [43] limited to nearly complete rRNA gene sequences of known prokaryote origin (i.e., excluding sequences determined from bulk environmental DNA) returned 9,093 records for small subunit rRNA (> 900 nucleotides in length), and 222 records for large subunit rRNA (>2000 nucleotides in length). Even though these numbers overestimate the complete number of prokaryote rRNA gene sequences in GenBank, they provide a rough estimate of how rare rDNA introns are in prokaryotes. It is therefore surprising to find three group I introns with HEGs in a single rDNA gene (in Synechococcus sp. C9). It is unclear why Synechococcus sp. C9 contains three introns and S. lividus strain C1 contains two, whereas the vast majority of bacteria contain no rDNA introns and the few others that have any introns possess only one.
One possible explanation is that the life history and/or physiology of this cyanobacterial group promote intron transfer. Alternatively, introns may sometimes serve a role in the host cell and therefore accumulate in these lineages. Whatever the reason, once inserted into rDNA, introns could pose a risk for bacteria because they could potentially interfere with posttranscriptional processing of precursor rRNA transcripts. Although not fully understood, this processing is relatively complex in bacteria [e.g., [44-46]]. In addition, group I ribozymes catalyze side reactions other than self-splicing, reactions that result in intron RNA circles and fragmented rRNAs [47]. Some rDNA operons and primary transcripts contain many group I introns (e.g., the rDNA operon of the myxomycete Fuligo septica harbors 12 group I introns [48]), which makes it increasingly important to strictly regulate group I ribozyme activity towards splicing and not circle formation.
We found multiple HEG-containing group I introns in cyanobacterial LSU rRNA genes. Specifically, the LSU rRNA gene of Synechococcus sp. C9 contains three group I introns, at positions L1917, L1931, and L2593, whereas the S. lividus strain C1 LSU rRNA gene contains similar introns at L1931 and L2593. This finding is surprising because the vast majority of bacteria contain no rDNA introns and the few others that have any introns possess only one. The intron-encoded HEGs belong to the LAGLIDADG family, and contain one copy each of the conserved amino acid motif that defines this family (i.e., the LAGLIDADG motif). Phylogenetic analyses show that the cyanobacterial introns and their HEGs are closely related to introns and HEGs located at homologous insertion sites in organellar and bacterial rDNA genes. Finally, from previous studies it is estimated that approximately 3% of nuclear group I introns contain HEGs. In our survey of group I introns and HEGs in the literature we estimate that at least half of organellar group I introns contain HEGs, and that about one third of bacterial group I introns contain HEGs.
Bacterial strains and nomenclature
Axenic slant cultures of Synechococcus lividus strain C1 and Synechococcus sp. C9 were a gift from David Ward, MontanaState University, Bozeman. These cyanobacterial strains wereoriginally isolated from microbial mat communities in Octopus Spring, Yellowstone National Park, Wyoming, U.S.A. Cells were scraped fromthe slants and DNA was isolated with the Puregene kit (GentraSystems, Minneapolis, MN) following the manufacturer's protocol.
The bacterial nomenclature used in this study is not addressed here other than to point out that the cyanobacterial names in this paper are of botanical origin and have not been validly published under the rules of the Bacteriological Code, unlike the other bacterial names in this report. Therefore they should be considered ad hoc and not necessarily consistent with inferred phylogenetic relationships.
PCR and DNA sequencing
Approximately 2.8 kb of the 23S rRNA gene was amplified from genomic DNA by polymerase chain reaction (PCR) using primers 36F and 2763R [see Additional file 1]. Amplifications were carried out in 50 μL reactions under standard conditions in a PTC 200 DNA Engine thermal cycler (MJ Research). The reaction mixture typically contained 1.0 U of Taq Polymerase and 10× PCR buffer (Gibco BRL Life Technologies), 0.04 mM of each deoxynucleotide, 600 nM of each amplification primer, approximately 50 ng of genomic template DNA, and purified water to volume.
Additional file 1. Oligonucleotides used in this study. Primers used for amplication and sequencing 23S rRNA (including introns and homing endonuclease genes) in Synechococcus.
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Temperature and cycling conditions were as follows: one 95°C denaturation cycle for 3 min, followed by 35 cycles of 95°C denaturation for 15 sec, primer annealing at 49°C for 15 sec, and elongation at 72°C for 90 sec. Four μL of the amplified products were visualized on 1.5% agarose minigels and the remainder was purified using 30,000 NMWL low-binding, regenerated cellulose membrane filter units (Millipore). Agarose plugs were sometimes taken of weak PCR products and reamplified at 51°C using the same conditions. Both strands of purified PCR products were directly sequenced in 10 μL reactions using the sequencing primers listed in Additional file 1. Cycle sequencing was conducted using dRhodamine Dye Terminator reagents and a PE-ABI 377 automated DNA sequencer (Perkin Elmer – Applied Biosystems). Sequence fragments were edited and assembled into contigs using Sequencher 3.0 (Gene Codes). Sequences obtained in this study have been assigned GenBank accession numbers DQ421379–DQ421380.
Intron secondary structure prediction and GenBank searches
The central paired elements (P3, P4, P6, and P7) in group I introns were identified by comparing the intron sequences to available secondary structures of related introns (identified by BLAST searches). Secondary structures of peripheral regions were predicted using Mfold [49].
The number of available small and large ribosomal RNA gene sequences of known origin was determined by searching the NCBI (GenBank) databases, restricting the search to prokaryote organisms and excluding sequences determined from bulk environmental DNA. The search was further restricted to complete or nearly complete gene sequences, at least 900 nucleotides in the case of small subunit (16S) rRNA sequences and at least 2000 nucleotides in the case of large subunit (23S) rRNA sequences.
Phylogenetic analyses
The five Synechococcus intron DNA and HE protein sequences were added to previously published sequence alignments [35]. Only intron and HE sequences from homologous LSU positions were kept, and the final alignments contained 44 sequences with 139 nt and 136 aa, respectively [see Additional files 2 and 3]. Phylogenetic analyses were done as previously described [35], and will only be explained here briefly. A minimal evolution tree (WAG + Γ model) was inferred from the protein data set using the programs TREE-PUZZLE 5.0 (to calculate distances), and Fitch (for inferring the topology) from the PHYLIP V3.6a3 program package. TREEVIEW 1.6.6 was used to produce the tree image. Support for nodes was calculated with one bootstrap analysis (neighbor-joining, JTT-model, and 500 replicates), and Bayesian inference (WAG + Γ model, 2 million generations and 50,000 cycles as the burn-in). A 50% majority-rule consensus tree was inferred from the intron data set using Bayesian analysis under the GTR+I+Γ substitution model. The tree includes only those LAGLIDADG HEG-containing group I introns that are inserted at the same four rDNA positions (Table 1) at which introns are found in bacteria. Two sets of bootstrap values were calculated [minimum evolution (Jukes-Cantor model and 2000 replicates) and maximum parsimony (200 replicates)].
Additional file 2. Group I intron dataset. Nexus formatted intron data set.
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Additional file 3. Homing endonuclease data set. Nexus formatted homing endonuclease data set.
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HEG, homing endonuclease gene; HE, homing endonuclease; LSU, large subunit, rRNA, ribosomal RNA; rDNA, ribosomal DNA; ORF, open reading frame; SSU, small subunit.
Authors' contributions
PH reconstructed the putative secondary structures of rRNA group I introns in Synechococcus strains, carried out the phylogenetic analyses, compiled the list of group I introns with HEGs in bacteria, and drafted the manuscript. DB participated in the analysis and interpretation of the data and in manuscript preparation. JDP conceived of the broader study using LSU rDNA to examine the phylogeny of cyanobacteria, and assisted with data interpretation and manuscript preparation. ST participated in the broader phylogenetic study, in the experimental design that led to the discovery of the introns reported here, in GenBank searches, and in the drafting of the manuscript. LAL assisted with data analysis and interpretation, and in manuscript preparation. KMP planned and coordinated the study, designed primers, sequenced the LSU rRNA data reported here (including the introns and their encoded HEGs), and assisted with drafting the manuscript. All authors have read and approved the final manuscript.
This work was funded by NIH grant GM-70612 to JDP. KMP is grateful to Jeremy Kirchman and Elizabeth Grismer for laboratory assistance and to the Pritzker Foundation Fund of The Field Museum. PH and DB acknowledge generous support from the NSF (MCB 0110252). The contribution by ST to this research was supported in part by the Intramural Research Program of the NIH, National Library of Medicine. We thank Steinar Johansen and Dawn Simon for comments on the manuscript, and David Ward for providing cultures of Synechococcus lividus strain C1 and Synechococcus sp. C9.
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Self-Medication makes electroacoustic / indietronic music, with some influence of glitchiness and experimental noise. From Toronto, though recently in Taipei.
His lack of instrumentation in Taiwan led to remixes of
Self-Medication tracks and other artists, as 'Re-Medications'. It also sprouted a new-found interest in the mixing arts, of which some of the results can be heard here.
Tube of You:
Book of Face: https://www.facebook.com/Self.Med...
Cloud of Sound: http://soundcloud.com/selfmedication
And twittles too: https://twitter.com/#!/_Self_Medica_
So, take a listen, comment if you will, have a snack, maybe read a nice book, and Enjoy. 謝謝!
Press play to try again.
You can get it here.


text:
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Tommy Ryan (Character)
from Titanic (1997)
FilmographyEdit Credits
- Titanic (1997) Played by Jason Barry
... aka "Titanic in 3D" - USA (3-D version)
... aka "Titanic: The IMAX Experience" - USA (IMAX version)
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Tommy Ryan: That's typical. First class dogs come down here to take a shite.
Jack: That's so we know where we rank in the scheme of things.
Tommy Ryan: Like we could forget.
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text:
The Autumn Arts Festival
at the Dievole Chianti Classico Winery
October 20-29, 2015
Tuscany in autumn is a sensual feast. Russet and golden hills. Crisp air. Trees weighted with olives. Woods rich with mushrooms and chestnuts. The scent of grapes hangs over the valleys like mist.
The harvest is the soul of the Tuscans. In autumn the hilltowns revert to the locals. Their art is to prepare the bounty: prosciutto, nuts, olive oil and especially Chianti. Traditional festivals celebrate every phase from the vendemmia (harvesting of grapes for wine) to the wild boar. Music, folklore, dancing in the piazza.
And there, in the heart of Tuscany, is Siena. The legendary medieval town packed with history and art. In autumn, her museums and churches relax, uncrowded. The streets echo her architecture, art and heroes.
Since 1998, Il Chiostro has celebrated this time of the year in Tuscany with our final program of the year - The Autumn Arts Festival. The people of Dievole have become like family to us and our guests: Dino roasts the pigs in an over built in the 1700`s, Silvano prepares our welcome appetizers to greet us on the first day of arrival, Leonardo waits for us to pick his olives so he can teach our guests the technique that damages the olives the least, and Enzo the chef creates a magical meal based on the bounty of the season that we can enjoy dining in the vecchia cantina - the historic wine cellar from the original Dievole vineyards.
Painting, Photography, Tuscan Cuisine plus Italian Opera Appreciation
For the Autumn Arts Festival we offer 4 separate workshops - Painting, Photography and Cooking - that all overlap throughout this 10-day celebration of the arts, the harvest, the wine and this wonderful region of Italy. In addition we offer Italian Opera Appreciation each day that is open to everyone. For details about those workshops, click on the links below.
Select one of the following workshops as your primary activity during the festival:
- Painting in Watercolor & Acrylic with Lynn Stender
- Tuscan Cuisine with Linda Mironti
- Creative Photography Explorations with Dan Burkholder
- Encaustic and the Photographic Image with Jull Skupin Burkholder
Open to everyone is:
Dievole Wine Bottles by Gilbert Rios
Excursions and Activities
Generally your workshop will shape your activities at the Autumn Arts Festival. For example the cooking class may go olive picking while the painters spend the day in Radda painting the vistas of the Tuscan hills and the photographers travel to Montalcino or Pienza for a full day shoot. Then everyone will return to the winery in the evening for the opera class and dinner together.
But during the 10 days there will be several activities and excursions for everyone to enjoy. Here is a partial list that you can look forward to:
- a wine tour and tasting throughout the Dievole complex to give you a good understanding of Chianti`s greatest treasure, red wine.
- a full day excursion to Florence with an option to go to the Uffizi art gallery or to see Michelangolo`s exquisite sculpture of David.
- a mid-week pig roast - a harvest feast in the tradition of a typical Tuscan celebration
- a visit to the medieval city of Siena for time to explore the Duomo, the art museums, sit in the Piazza del Campo with a cappuccino or just shop the elegant stores along the main pedestrian street
- dinner in the old wine cellar, with local musicians and the chef`s most special meal from antipasto to dolci.
- a half day trip to the hilltown of San Gimignano, famous for the many medieval towers that still puncture the skyline
- plus some other surprises that don`t want to give away before you arrive!
Halloween pumpkins, Halloween coven
Lunch overlooking Montalcino, a photo op in Florence (photos by Alan Kuhlow)


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Archives for "Sociology"
February 20, 2003 12:00 pm
Bates hosts major conference on Lewiston-Auburn diversity
Well-known Maine politicians, officials from the public and private sectors, and local Somali residents will join educators and students at Bates College in March for the conference "Toward Harmony: A New Diversity in Lewiston-Auburn."
January 22, 2003 2:45 pm
Jean Kilbourne, ad critic and 'Lecturer of the Year,' to speak
Internationally recognized for her pioneering work on how advertising represents alcohol, tobacco and the image of women, author Jean Kilbourne offers a slide presentation titled "The Naked Truth: Advertising's Image of Women."
September 25, 2001 8:39 am
Gender issues expert continues "Difference" lecture series
Noted sociologist and writer Allan Johnson will discuss issues of social inequality and privilege in his lecture, "Privilege, Power, and Difference," Tuesday, Sept. 25, in the Bates College Edmund S. Muskie Archives.
May 4, 1998 1:01 pm
Child welfare advocate to speak
Michael Petit, deputy director of Child Welfare of America, discusses Breaking the Link Between Child Maltreatment and Youth Violence Thursday, May 7, at 3 p.m. in Chase Hall's Hirasawa Lounge. Petit's talk is sponsored by the psychology and sociology departments, as well as Advocates for Children. The public is invited to attend free of charge.
August 12, 1997 11:48 am
Farewell lunch to be held for Seeds of Peace group
A delegation from the Seeds of Peace International Camp will attend a farewell luncheon and discussion hosted by Bates College at noon on Thursday in the Benjamin Mays Center.


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Plymouth Jacket & Cardigan Patterns - 2699 Slip Stitch Long Cardigan and Blue Sky Alpacas Adult Clothing Patterns - Autumn Vest are both similar patterns: 11 (2.75 sts. / inch) finished gauge, for women, and bulky (12-15 sts/4 in) yarn gauge.
Hopefully our suggested yarn options for Yoga Bags and Everything Totes can help you come up with your next exciting new project idea!


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13 CFR 120.348 - Amount of guarantee.
The maximum loan amount for any one International Trade (IT) loan is $5,000,000. IT loans may receive a maximum guaranty of 90 percent or $4,500,000, except that the maximum guaranty amount for any working capital component of an IT loan is limited to $4,000,000. To the extent that the Borrower has a separate EWCP loan or any other 7(a) loan for working capital, the guaranty amount for the other loan is counted against the $4,000,000 guaranty limit for the IT loan.
[76 FR 63546, Oct. 12, 2011]
Title 13 published on 2014-01-01
The following are only the Rules published in the Federal Register after the published date of Title 13.
For a complete list of all Rules, Proposed Rules, and Notices view the Rulemaking tab.
Title 13 published on 2014-01-01
The following are ALL rules, proposed rules, and notices (chronologically) published in the Federal Register relating to 13 CFR 120 after this date.


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About Lone Star Sandblasting & Painting
Specialized, heavy-duty machinery, equipment, and structures are a substantial investment for any company. Lone Star Sandblasting and Painting helps you experience a higher return by extending the life and increasing the value of the assets you rely on to work effectively by preventing corrosion. Our expert crew is fully-trained and armed with the most advanced technology and techniques to meet the demands of any job. Lone Star Sandblasting and Painting is committed to delivering the highest-quality results and best customer experience in the industry.
What We Do:
Lone Star is hungry for growth and appreciates our business, rather than making us feel like we're being done a favor.
- David Smith, Nello Corporation – Quality Control Supervisor
The quality of work, delivery of commitments, and great attitudes makes it easy to recommend Lone Star Sandblasting and Painting.
- Brett Dupes, Larrett Energy, Project Manager/Superintendent
I would and have recommended Lone Star to our employees as well as other contractors due to Lone Star basically "taking care of business" and making it one of the easier parts of our projects.
- Chris Parker, Larrett Energy Services, North Texas Area Manager
Their team had an eagerness to accommodate our needs, and a willingness to go the extra mile. These qualities make Lone Star Sandblasting and Painting a highly recommended company on our list.
- Danny Long, Atmos Energy, Compliance Supervisor
Opening its doors in 2004, Lone Star Sandblasting and Painting quickly established itself as a leading name in the industry, thanks to cutting-edge equipment, rigorous training and safety protocols, highly professional crew members, and the relentless pursuit of excellence.
We provide the following, serviced at our location or yours:
- Expert Sandblasting
- Painting and Specialty Coatings
- Plural Component Systems
- Tank Linings
- Hotshot Pick-up & Delivery Services – On-Site and Field
- Heavy-duty Equipment
- Factory Machinery
- Pipelines
- Permanent Structures
- Tanks
- Vessels
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- Buildings
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- Compressor Stations
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Our experience in blast media includes:
- Sand
- Steel
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- Garnet
- Black Diamond
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- Soda
- Wet and dry
In addition to abrasive blasting, we apply appropriate paints and coats using brush and roll or spray techniques. Protecting your equipment and extending its life and value is our top priority.
Where We Work:
Lone Star Sandblasting and Painting works where you need us. We operate a fully-enclosed 12,000 square-foot shop in Mansfield, Texas. Equipped with the latest technology, it operates under strict quality and safety standards to ensure the highest-quality results. We are also equipped with mobile crews who can travel to you.
From the Marcellus Shale in the Northwest US to the Bakkan Shale in the Dakotas to all of the Shale plays in the southwest US (Haynesville, Barnett, Cline Eagleford, Woodford), we work for clients all over the United States. Our clients include Exxon, Atmos, XTO, Koch Pipeline, Trinity, Trans Union, Army Corp of Engineers, Nello, EOG, MIINC, Flint Hills, Well-done Rig Works, Consolidated Rig Works, Total Equipment, Valmont, Sabre, AZZ Galvanizing, Enbridge, Crosstex, Chesapeake, Devon Energy, the DFW Airport, Halliburton, and many more rely on the convenience and professionalism that Lone Star Sandblasting and Painting brings to the field.
Who We Are:
Led by owner Paul Lajoie, Lone Star Sandblasting and Painting has an unparalleled track record of quality work and outstanding customer service. Field and shop crews, operators, and supervisors are fully trained and certified. Daily "tailgate" trainings keep team members current on the latest techniques and equipment, and random-selection DOT drug testing ensures a high level of safety and professionalism.
- Workers compensation – $1,000,000
- General liability – $1,000,000, $2,000,000 aggregate
- $5.0 million umbrella policy
- Auto – $1,000,000, $2,000,000 aggregate
- Business Personal Property
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Search the collections
Where is it from?
- A Collector Melbourne, Australia
- B Manufacturer London, United Kingdom
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Case - Pair of Pistols, Wogdon, London, Flintlock, circa 1790 Reg. No: ST 021858
- Summary:
- Flintlock duelling pistols, cased pair with accessories, cal. 25 bore (.546), smooth bore, swamped octagonal damascus barrels, browned, 25.40cm long with gold brushed vent.
Made by Robert Wogdon, London, circa 1790.
- Description:
- Barrel attached to stock by two fixing keys. Steel side lock with saftey catch and hammer on R.H.side, steel triggerguard with spur and acorn extension, light remnants of blueing, hair trigger, and ramrods mounts. Back sight is a wide U notch set on tang, brass bladed front sight set in top barrel flat. Wooden stock with flat sides on grip extends almost to muzzle, semi-round butt with steel butt cap foliate engraved, wooden ramrod. One ramrod has a horn end, the other has been replaced at some point with a wooden one.
Oak case with brass fittings, handle and escutcheon inlaid on lid engraved 'I.M.'. Lined with a course green lining, two opposing side compartments with cast lead bullets and wadding swatches, and a central compartment containing powder flask and pewter vent hole cleaner with prick. Lining is of a coarse material and shows colour variation in places, suggesting it may have been relined, possibly even within the item's working life. No trade label attached to the inside of the lid, which is characteristic for Wogdon.
- Acquisition Information:
- Donation from Mr William Cole, 1943
|75 mm (Height), 450 mm (Width)
More information
|Tagged with:
|pistols british
|Themes this item is part of:
|William Cole Arms Collection, Robert Wogdon, Gunmaker, London (1737-1813), William Edwin James Cole, Gun Collector (1864-1952), Arms Collection
|Primary Classification:
|Secondary Classification:
|Tertiary Classification:
|Engraved on lock plate: Wogdon
Engraved on top barrel flat: Wogdon LONDON
Engraved on brass escutcheon inlaid in case lid: I.M.
Former catalogue record noted London proof marks and maker's mark 'R.W'. These are not visible on outer surfaces at the time of this record enhancement (13/4/05) and may be under barrel.
|Model Name/Number:
|Robert Wogdon (Gunsmiths), London, England, Great Britain, circa 1790
|Mr William Cole, Melbourne, Victoria, Australia
This item is part of the following themes:
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22 year old woman | Saint Augustine, FL, USA | Seeking men 20-24 within 50 miles
What's a life worth living if you're not on the edge?
My life is super fast paced, but I like to find time to enjoy the life I have. I'd like to find someone who wants to spend time with and explore the world with. I'm an easy going, fun loving girl who likes to laugh. I love photography and video editing. I'm studying Media Production in the Communications field. I listen to a ton of music. I'm hard working and driven and majority of what I do is goal oriented. I support myself 100% percent and pay all my expenses. I don't rely on anyone but myself and pride myself on my independence. I have a bright and animated personality and I can be kind of a dork sometimes. Also, I love cats. I love letting my creative side show in everything I do. People wouldn't guess it, but I'm a huge hockey fan. New York Rangers have been my team since birth. I'm a radio DJ on Monday mornings from 9-11am on WFCF 88.5, feel free and take a listen, I play good music. When I'm not working 30 hours a week, I'm either out working on some kind of project, dancing and doing event photography at my local nightclub on Mondays, at taking a drive while listening to my music or home watching a movie with my cat Dahlia. Ideally, I'd like to go out more to see and try new things. Oh, I'm learning to kitesurf too! The ideal guy for me would have to be tall with a great smile. He has to have a really great taste in music, preferably similar to mine, with a good sense of humor. A guy who is artistic or creative and could even be romantic sometimes. I like someone who's not overbearing, into drugs or constantly talks about religion or politics. Someone who knows how to cook is a bonus. I need someone who shares the same hardworking personality as me and knows where they want to go in life. Love and support is all I ask. Books: I wish I had the time to read more. I like to sink into a good fictional book, some sci fi, fantasy, horror, romance. I like to read a lot of current news. Movies: Definitely a lot of horror and psychological thrillers, I like a good comedy or intense drama, historical films are good, independent films, I like my share of action and adventure, musicals, sci fi, a few animated films. Shows: I don't watch a lot of tv, but anything on the discovery channel, paranormal shows, True Blood, Blue Mountain State, How I Met Your Mother, 30 Rock, majority of shows on the food channel, Futurama. I probably forgot list a few others. Music: Oh my, where do I start? I have a crazy eclectic taste in music. I like a lot of rock, Deftones, He Is Legend, Queens of the Stone Age,. I've gotten into a lot of post hardcore lately, Bring Me The Horizon, Architects, Chiodos, etc. But I like a lot of pop rock too, Fall Out Boy, Blink 182. Love my classic rock, The Beatles, Led Zeppelin, Guns N Roses, Van Halen. I'm a sucker for some Jazz or Big Band. I also like some good folk sounding acoustic indie stuff, Margot and the Nuclear So and Sos, Cloud Cult, Iron and Wine, City and Colour. Sometimes, I'll listen to some techno, dubstep sounding stuff, DeadMau5, David Guetta. I've gotten into some Pop, Ke$ha, Lady Gaga, Nicki Minaj, Rhianna, Katy Perry. Punk/Ska is good too, Streetlight Manifesto, NoFX, Reel Big Fish, Rancid. If I listen to country, it's very rare that it's the newer stuff like Lady Antebellum, and it's more like Hank Williams or Wayne Hancock. And I've got a million other little bands in between, The Strokes, The Donnas, MC Chris, Panic at the Disco, etc. Food: Gotta stick with my Italian roots, but I love some Sushi or Japanese food. I'm a steak and potatoes kind of girl.
Coffee and conversation , Dining out , Hobbies and crafts , Movies/Videos , Music and concerts , Exploring new areas , Nightclubs/Dancing , Performing arts , Travel/Sightseeing , Video games
Dancing, Yoga
I exercise 1-2 times per week.
I have Cats
I like Dogs, Exotic Pets, Fish, Horses
I have a cat named Dahlia who will be a year old in April.
I spend a lot of time in downtown St. Augustine, but I love the beach and various state parks like Favor Dykes or Princess Place Preserve. Every Monday im at Johnny's/Club Fusion doing event photography.
Flagler College, St. Augustine, FL
I love comedies, horror, action and drama. I listen to too much music, from top 40s, edm, dubstep, pop, rock, punk, blues, jazz, post hardcore, metal, and a number of other things. I love Italian food, sushi and I'm a steak and potatoes kind of girl.
I do a lot of photography and video editing. I like going out dancing and spending time with friends. I like to take drives and listen to music. Making diy crafts is a lot of fun too. Day at the beach and hikes are great as well.
Some current event news online.
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What she is looking for
|5'0" (152cms)
|5'7" (170cms) to 6'5" (195cms)
|Body type:
|Slender, About average, Athletic and toned
|Blue, Brown, Grey, Green, Hazel
|Dark brown
|Auburn / Red, Black, Light brown, Dark brown, Blonde, Dark blonde
|No Way
|No preference
|I'll tell you later
|No preference
|I'll tell you later
|No preference
|$25,001 to $35,000
|Less Than $25,000, $25,001 to $35,000, $35,001 to $50,000, $50,001 to $75,000
|Never Married
|Never Married
|Have kids:
|Wants kids:
|No preference
|Latino / Hispanic, White / Caucasian
|White / Caucasian, Other
Bolivian and Italian decent
|Spiritual but not religious
|No preference
|Some college
|Some college, Associates degree, Bachelors degree
I'll be a senior at Flagler College in the fall. I'm a Communication major with a Media Production track and an Advertising minor. Everything from video to audio editing and film making, that's what I do.


text:
Become an Epidemiologist: Education and Career Roadmap
Research the requirements to become an epidemiologist. Learn about the job description and duties, and read the step-by-step process to start a career in epidemiology.
Do I Want to Be an Epidemiologist?
These scientists are often associated with studying contagious diseases, though they're also involved with other public health issues, including chronic disease, maternal health and substance abuse. Epidemiologists may work for public and private health institutions, government agencies, laboratories, pharmaceutical businesses or universities. They conduct surveys and analyze body fluids to determine disease or illness outbreak patterns, and then they strive to control spread of disease and prevent future occurrences of illness through public health programs. Safety procedures must be followed carefully when dealing with potentially infectious disease.
Job Requirements
Most epidemiologists have a master's degree, but those who conduct research for universities or have senior-level jobs often need a Ph.D. Some epidemiologists have professional medical backgrounds. The following table contains essential requirements for epidemiologists:
|Common Requirements
|Degree Level
|Degree Field
|Epidemiology, public health*
|None for entry-level positions; advanced-level jobs require 5 or more years working in the field**
|Key Skills
|Research design, written and spoken communication, attention to detail, critical thinking, statistics*
|Computer Skills
|Microsoft Excel, scientific software, map creation software, query software, statistics software, data analysis software***
Sources: *U.S. Bureau of Labor Statistics, **Online job postings (September 2012), ***O*NET Online.
Step 1: Complete a Bachelor's Degree Program
Aspiring epidemiologists must first earn a bachelor's degree. No specific major is required, though undergraduate coursework should include at least one class each in biology, chemistry, calculus, health science, social science and behavioral science.
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A master's degree in epidemiology or public health is generally the minimum educational requirement for epidemiology careers. Curricula tend to draw from a variety of disciplines, and they typically cover topics such as epidemiologic research methodology, clinical trial design and biostatistics. Additional topics of study may include society and health, medical geography and occupational epidemiology. A thesis is required to graduate. Students may be able to focus their research in areas like genetic epidemiology, cancer epidemiology, cardiovascular epidemiology or neuroepidemiology.
Some programs have specialty clinical research emphasis options. Students may be able to focus their epidemiology research in professional fields like medicine, dentistry or pharmacy. In fact, some of these are designed only for current medical doctors or medical school students.
Success Tip
- Become well-versed in relevant technology and software. Employers may give preference to candidates who are familiar with statistical analysis and data presentation software. Epidemiologists can consider learning programs like SAS/GRAPH and STATA. Other software programs that epidemiologists may consider learning include World Health Organization HealthMapper and CDC WONDER.
Step 3: Earn a Doctoral Degree
A Ph.D. may be required for career advancement to high-level research positions and university teaching jobs. Students are expected to focus on an epidemiology specialty, such as nutritional epidemiology, chronic disease epidemiology or molecular epidemiology, though curricula are flexible in order to meet students' personal interests. There are also dual M.D/Ph.D. programs in epidemiology that highlight clinical research. All epidemiology doctoral students must complete original independent research and write a dissertation in order to fulfill graduation requirements.
Related to Become an Epidemiologist
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this could be the end of the fat african.Posted February 12, 2014 3:16 pm
I`m your daddy
TARK, you are a daft old tart aren`t you? Aren`t you?
Yes you are. Yes you ARE, you big old daft old tart you.
COme here, come here, who wants their scraggyold belly rubbed, that's right you do, right before Mork plunges his shaft between your quivering buttocks and rearranges your intestines to match the London tube map.Posted February 12, 2014 1:23 am
JE, I agree that is totally unknowable. Perhaps a Soveiet fighter or 3 would have been very dominant if it hadn't been for the Iron Curtain or WW2 etc. The Klitschkos would have been greatin any era. Perhaps the late 1960s Ali would have beaten them but we will never know. They are both certainly top 10-15.Posted February 11, 2014 7:18 pm
Tony — The dirtly little secret about the heavyweight division is that it has always been thin. You're right that way too many people assess this era is somewhat less than others, but I just can't see too many champions or contenders from past eras punishing Wladimir Klitschko or his brother. One other key thing to consider is that heavyweights in the prior to the 90's never faced anyone from eastern Europe in the professional ranks. Not even the greats like Louis, Marciano, Ali, Foreman, Frazier etc… can claim even a single title fight win over anyone from behind the Iron Curtain. While it is unknowable how it would have changed the face of heavyweight boxing, it is almost certain that things would've been somewhat different, just as it will be different when or if Cuban fighters routinely turn pro.Posted February 11, 2014 6:24 pm
Rustybucket – BTW it is not disrespect to accurately assess the relative ranking of a pro fighter. In this everybody-gets-a-trophy world it is increasingly politically incorrect to even give a ranking, but in boxing the results in the ring give us reasons to make judgments. If we're wrong it will be borne out in the ring.Posted February 11, 2014 6:17 pm
I agree with Rusty. Certainly Chisora and Kingpin aren't all time greats, but they may be as good as Bugner, Mildenberger, Roper, and a hell of a lot of other people who were contenders of their day. There is a tendency to downplay the talent of today but even in the HW high era of the 1970s, after the top 5 or so, the talent was thin then too.Posted February 11, 2014 6:15 pm
Which world title are they credible contenders for? Neither came close to winning titles when they had their respective chances. Chisora was crushed by Haye since, and Johnson was clearly beaten by Hammer and Fury, and Hamer in a pseudo pro match in the Prizefighter tournament. Yes they are perhaps slightly above club status but not by much.Posted February 11, 2014 6:14 pm
Way too much disrespect for fighters these days, Both these guys are NOT club level, They are contenders for a World Title.Posted February 11, 2014 5:38 pm
The hype for what is essential as solid club fight is amazing but not unexpected. Neither Chisora or Johnson present the least bit of threat to either of the Klitschkos and won't win a world title no matter the outcome of this bout. Nonethless, it is probably evenly matched and the outcome is at least not obvious. It could be a good fight if Johnson comes with the idea that he wants to win and not just survive. If he goes into a shell look for a repeat of his bout with Vitali Klitschko.Posted February 11, 2014 2:00 pm
Kingpin is like Joe Bugner. He loses to guys he shouldn't lose to. Bugner lost to guys like Middleton and Johnson lost to Hammer. They don't KO too many people, but they hang in there against the best.Posted February 11, 2014 1:39 pm
keep the hw circus rolling.Posted February 11, 2014 1:13 pm
Oh please, can't they just all punch themselves in their own chin and get KO'ed. Would put an end to the misery.Posted February 11, 2014 9:02 am
I agree with Farmboxer that Wilder choose Scott because he is not a big puncher and his loss to Chisora didn't hurt but to say Scott will lay down is stupid. Scott will try to win because a guy who is 36-1 if he beats Wilder that puts him in for a big fight. He'll try to win. We should find out a little more about Wilder. But I bet he blasts out Scott faster than Chisora.Posted February 11, 2014 8:11 am
True, if Chisora wins by stoppage that will be a real upset.
I like the way Chisora has got things on track last year and hope he can continue to improve, what he really needs now is more snap to his punches, as he's not naturally heavyhanded.Posted February 11, 2014 6:25 am
the voice of reason
if chisora stops johnson he'll make a believer of me cos the guy is VERY durablePosted February 11, 2014 6:02 am
Johnson took this fight on short notice, but I doubt if Chisora can knock him out…………….however, Chisora had no problem knocking out Scott, that's the reason that Wilder decided to fight Scott, a boxer without a chin, no stamina, punches like a woman………….Wilder knows that, plus Scott is his best friend, so, will Scott do Wilder a favor? Think about it………..Posted February 11, 2014 1:10 am
Chisorа is а bumPosted February 11, 2014 12:52 am
Alex Leapai vs Travis WalkerPosted February 10, 2014 10:54 pm
Im not so sure. He best have improved his punchPosted February 10, 2014 10:10 pm
Tony g spot on my friend I hope johnson really wants this or rather he's had some notice on this fight which I doubt so he probably only had two
Notice if well preped johnson pisses it but we know they wouldn't take it like thatPosted February 10, 2014 9:43 pm
This will be a dreary fight… KJ won't do a thing… Chisora will swing at air for the duration and be awarded a victory for shadow boxing and pushing.Posted February 10, 2014 6:59 pm
dyson fury and chisora on the same card. bet the heavyweight junkies cant wait.Posted February 10, 2014 4:30 pm
Mr gav
If chisora wins we get fury chisora 2 if Johnson wins we get fury Johnson 2! So let's hope chisora wins, Johnson fury 2 can't be any worse than the first one can it?Posted February 10, 2014 4:25 pm
third world
kingpin all dayPosted February 10, 2014 4:21 pm
at least johnson has got the right american name.Posted February 10, 2014 4:03 pm
Blast out Johnson? Wouldn't be surprised if Johnson wins this fight. A fit and well prepared Johnson is on a higher level than Chisora is in boxing terms. I'll be surprised if Chisora wins at all, let alone blast out Johnson. Johnson has a very good long jab, and a bit of power to keep Chisora in check.Posted February 10, 2014 4:01 pm
It can be a dangerous fight for Chisora…if…Kevin Johnson has trained well and comes to box with winning in mind, otherwise, no, Chisora will probably collect a dull decision. You never know though, with some of the referee's doing Dereck's home fights recently, they might DQ Kevin for hitting him!Posted February 10, 2014 3:58 pm
martins a muppetPosted February 10, 2014 3:38 pm
Mr gav
If chisora goes for the knock out, he will find out he has not got the power,the stamina or skill to do so and may even get knocked out himself. For a fighter of chisoras limitation this is a dangerous fight. If he fights smart he can win easily but not excitingly. And seeing as though Johnson tko over Alex leapai he has enough power to tko a very tired chisora in the late rounds.Posted February 10, 2014 3:24 pm
Not a chance Chisora gets Johnson out of there… people nicknamed him Safety Pin for a reason. If I were Chisora I'd just focus on winning in general coz KJ can be a tricky one if he comes in motivated.Posted February 10, 2014 3:16 pm
Chisora isn't going to knock out Johnson. Johnsons defense is too good to get stopped but his offense is slow enough that he will probably lose on pointsPosted February 10, 2014 3:07 pm
too bad Johnson never developed a good offense. he has been doing this long enough.Posted February 10, 2014 2:46 pm
"I expect Chisora to maul us all to sleep ."
Yes, heavyweight boxing is great for getting the little ones off to sleep these days, I find.Posted February 10, 2014 2:45 pm
Shatterd Dreams
I expect Chisora to maul us all to sleep .Posted February 10, 2014 2:41 pm
i dont think david price has one yet.Posted February 10, 2014 1:47 pm
are there any heavyweights today that dont hold a belt. lol.Posted February 10, 2014 1:38 pm
Chisora is delusional if he thinks he can KO Johnson. He has never been stopped, and is one of the very few opponents that even Vitali couldn't put away. In fact, he kept teasing Vitali in the last round, beckoning him to hit him harder. So if Vitali, with his 90% KO record, couldn't so it, Chisora has no chance whatsoever. He may win the fight, but it will be dull, and there'll be no "blasting out".Posted February 10, 2014 1:31 pm
kevin johnson aka "safety pin"Posted February 10, 2014 1:12 pm
Johnson has a lot of skills to be taken out that easy. Vitali could not do it I wonder if Chisora can do it. It will not be easy at all.Posted February 10, 2014 12:58 pm
Brazilian Boxing Fan
This Saturday will not be September 15, it will be February 15.Posted February 10, 2014 12:56 pm
Brazilian Boxing Fan
I hope Chisora beats Johnson.Posted February 10, 2014 12:55 pm
I hope he does. I am tired of Johnson. He is all talk.Posted February 10, 2014 12:48 pm


text:
The best Mac HDR app for m4/3?
Started Sep 19, 2011 | Discussions thread
Junior Member • Posts: 30
gisligun wrote:
iharley wrote:
I second Photomatix Pro. Though I'll admit it took me a while to get the hang of it.
Photomatrix pro, its strange at first but you get the hang of it fast.
Made from 5 exp, from Olympus PL1 and Lumix 20mm
Photo missing fro mfirst post
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