text:
Canadians love to explore new flavours and spices. But when it comes to Thanksgiving, they want to tuck into the classics,…› More
Tagged: Thanksgiving
Just in time for the Thanksgiving long weekend, we've put together a round-up of some of our favourite recipes and…› More
If you're expecting to see yams on the Thanksgiving dinner table this year, you're not alone. They're a…› More
We've put together a Thanksgiving-inspired collection of cranberry recipes, including Pear Cranberry Chutney, Harvest…› More
LONDON, Ont. — Many family favourites on Thanksgiving dinner tables across Canada trace their origins to food made by…› More
Melissa D'Arabian/The Associated Press Let's talk about one of the season's most iconic vegetables —…› More
WASHINGTON — Thanksgiving tends to be a pretty full day for U.S. President Barack Obama, what with the annual ritual of…› More
TORONTO — LeBron James scored 27 points and Dwyane Wade added 22 as Miami hung on to defeat the Toronto Raptors 90-83…› More
OTTAWA — Only hours more to wait, for the Dec. 1 hockey date that's long been circled in red. That makes this…› More
Green Bay Packers guard Josh Sitton had some choice words for the Detroit Lions during a local radio interview. As he babbled…› More


text:
Women's Golf
Schooner Fall Classic
Sat., Oct 5
All day
All day
Norman, OK
Belmont Golf Club
Belmont Golf Club
Norman, OK
Watch Info
Not Available
Not Available
Not Available on App
Not Available on App
Not Available on App
More: Schooner Fall Classic - Women's Golf
NCAA Championships Within Reach for Women's Golf
Led by senior Noemi Jimenez, the No. 19 Arizona State women's golf team is two spots from qualifying for its 24th consecutive NCAA Championship. Rebounding from a first-round 296, the Sun Devils posted a 6-under 282 on Friday at the par-72, 6,350-yard Briggs Ranch Golf Course in San Antonio.
Vaughn In Hunt at NCAA Regionals
Arizona State's Monica Vaughn is four strokes off the lead after carding five birdies in the first round of NCAA Regionals on Thursday. The sophomore is tied for 24th with a 1-under 71 at the par-72, 6,350-yard Briggs Ranch Golf Course in San Antonio.
No. 19 Women's Golf Heads to NCAA Regionals
The No. 19 Arizona State women's golf team begins its quest for its 24th consecutive NCAA Championship appearance when it competes as the No. 5 seed at the NCAA San Antonio Regional, May 7-9. The Regional Championships will be held at the par-72, 6,350-yard Briggs Ranch Golf Club in San Antonio.
Women's Golf Seeded 5th in NCAA San Antonio Regional
The No. 19 Arizona State women's golf team earned the No. 5 seed in the NCAA Regional Championships to be held in San Antonio, Texas, the NCAA announced on the Golf Channel Monday evening. The Sun Devils will compete for an NCAA-record 24th consecutive bid to the National Championships.
ASU's Jimenez honored to be Scholar-Athlete of the Year
Arizona State's Noemi Jimenez is honored to be named Scholar-Athlete of the Year. She earned the award by maintaining a 3.8 GPA while playing on the women's golf team.
Jimenez Finishes Fourth at Pac-12 Championships
Noemi Jimenez recorded her fifth top-five finish of the season at the Pac-12 Women's Golf Championships on Wednesday. The Arizona State senior finished in fourth place with a 4-under 209 (69-70-70) at the par-71, 6,407-yard Boulder Country Club.

subset: commoncrawl


text:
Town Parc of Paris1715 North Main Street, Paris, TX 75460
12/04/14 by MountainMedic:
I recently retired after serving in the Army since 2001. I have rented in San Antonio, El Paso, South Carolina, Calcium NY, and have lived temporarily in 6 countries. I can tell you with complete confidence that this is a horrid place to bring your family. I'm not sure how the environment in an Apartment Complex became so combative but it needs to stop. There are warnings for children more
Regency Apartments2526 Lamar Ave, Paris, TX 75460
11/22/10 by sorrytoleave01:
we loved our apt Miss Lisa made it feel like everyone was family and moe was great anytime anything was wrong they took care of it right away I cried the first night I moved we had to move into a bigger place because of my health so people could stay with me and help me out we lived there for a little over a year we will miss everyone I have never had a more caring people as Miss Lisa and Mo they more
Meadowview Apartments520 Deshong Dr Ofc, Paris, TX 75462
04/23/12 by mdonian:
First of all the apartments rent for 350 per mo. So with that in mind ask yourself. Is this a good apt for the price.... yes it is. If u want to spend 600 a mo. Of course you will find better. Overall for 350 per mo. You can't beat that more
Park Garden North Apartments320 Stone Ave, Paris, TX 75462
07/05/11 by anonymous:
These are subsidized housing, they do have some rules and enforce them as needed. Maintenance keeps the place up pretty well and the staff are friendly and helpful. Laundry room is usually clean and well-kept. Management controls when the units change between heat and air. The only problems I've had at all are some of the people who move in are pretty rowdy. They don't seem to last more
Ridgeview Townhomes210 Stone Avenue, Paris, TX 75462
06/22/04 by happy4threeyrs:
I've been at Ridgeview since October 2001. The rent has only went up once. The staff is very friendly and helpful. If repairs are needed, they are done right then. I haven't had a problem with these apts and it's going on three yrs. I would not move for anything. more
Seasons Apartments2151 Bonham St, Paris, TX 75460
06/17/11 by jmueller27:
I loved this place top manager, great clean pools, private, shopping is convienent, bus service,kept-up grounds more
Westgate Apartments635 7th St Nw, Paris, TX 75460
01/25/12 by rafiqa:
this complex has the potenial to make money. the layout,the unit mix. first thing i would consider is putting a security fence around the complex. move the office from the main driveway, to the front build(leftside) of the driveway entrance. secure the air/cooling systems to the concrete. take one and two bdrms, at a time and gradually upgrade. make sure there,s security. more
Spanish Oaks Apartments3125 Clarksville St, Paris, TX 75460
06/10/14 by anonymous:
Spanish oaks is a slum. The staff is incompetent and lazy. There's trash everywhere. The property and laundry are gross. If you want to live in filth that is the place. The balconies and patios are falling apart around you. They never paint and think cleaning the carpet is prep for re renting. You have to be either dumb or stupid to rent here! more
Colonial Apartments3215 Clarksville St, Paris, TX 75460
03/01/02 by anonymous:
Don´t ever move here b/c they are the worst apartments u will ever live in more
Continental Village Apartments3275 Lamar Ave, Paris, TX 75460
07/19/12 by riverp8:
I think that my dad & I would like to live there. But, As far as I can tell, There is no office and no one ever returns your calls. Not sure how to get in! Don't know if I would recommend or not! more
Recently Rated Apartments in Paris


text:
Freeing John Sinclair Concert with Commander Cody Band and John Sinclair
December 10, 2011, marks the 40th anniversary of the John Sinclair Freedom Rally at Crisler Arena, held to protest the ten-year prison term given John Sinclair for the possession of two marijuana cigarettes (he was released soon after the Rally). On the evening before the anniversary, we'll celebrate the launch of our Freeing John Sinclair website (coming December 9!) with a FREE concert at The Ark featuring the Commander Cody Band (Commander Cody and the Lost Planet Airmen played at the original Rally), with special guest John Sinclair & Beatnik Youth.
Admission is FREE, and first-come, first-served, so get there early!
Friday, December 9, 2011 | 8:00 pm (doors open at 7:30) | The Ark | Ann Arbor
Yes, Get THERE! EARLY! People are coming from around the country to celebrate this event. Gary Grimshaw is hand painting the new poster as we speak! There is a reunion planned for the second weekend. This event is a highlight of two Friday nights and a Saturday. Cool stuff happening. Watch the site for more information. Put it on your calendar now! See you there!
Love on You *** Peace and Power
Gary & Laura Grimshaw, Detroit, MI


text:
Stores »
Such a fab gift!
Personalize everything without ink!
We are a community of shoppers sharing our best finds from across the web.
© 2015 ThisNext, Inc. ThisNext is a member of BUZZMEDIA Entertainment, a division of BUZZMEDIA.
Create your own free digital magazine at our new site Glossi.com


text:
noun in·ge·nu·i·ty \ˌin-jə-ˈnü-ə-tē, -ˈnyü-\
: skill or cleverness that allows someone to solve problems, invent things, etc.
: a clever device or method
plural in·ge·nu·i·ties
Full Definition of INGENUITY
obsolete : candor, ingenuousness
a : skill or cleverness in devising or combining : inventivenessb : cleverness or aptness of design or contrivance
: an ingenious device or contrivance
See ingenuity defined for English-language learners
See ingenuity defined for kids
Examples of INGENUITY
- She showed amazing ingenuity in finding ways to cut costs.
- It will take considerable ingenuity to fix these problems.
First Known Use of INGENUITY
circa 1592
Related to INGENUITY
Rhymes with INGENUITY
Learn More About INGENUITY
Next Word in the Dictionary: ingenuous (adjective)Previous Word in the Dictionary: ingenueAll Words Near: ingenuity
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What made you want to look up ingenuity? Please tell us where you read or heard it (including the quote, if possible).


text:
Latest China Oilfield Services Stories
GN Solids Control provide drilling mud recycling system and mud plant to COSL, which is the largest oilfield service company in China. (PRWEB) May 16, 2014
HONG KONG, Sept. 10 /PRNewswire-Asia/ -- The Board of Directors (the "Board") of CNOOC Limited ("the Company", NYSE: CEO, HKEx: 883) announces today that Mr. Zhong Hua will succeed Mr. Yang Hua as new Chief Financial Officer ("CFO") of the Company.
NEW YORK, May 12 /PRNewswire/ -- Atlantis Deepwater Orient Limited (ADOL) announced that a full scale trial of the innovative Atlantis Artificial Seabed System was successfully completed on April 27, 2009.
The China National Offshore Oil Corporation has reported total revenue of $4.5 billion for the third quarter of 2008, a 69.1% increase compared to the same period of 2007.
Awilco Offshore, through Premium Drilling, has received a letter of intent from the China National Offshore Oil Corporation for a two-year drilling program for the WilSeeker and the WilBoss jack-up drilling rigs.
CNOOC Ltd. posted a 89.3 percent profit increase in H1 BEIJING, Aug. 27 (Xinhua) -- China National Offshore Oil Company Limited (CNOOC Ltd.) said Wednesday that its first-half net profit rose sharply by 89.3 percent year on year amid high oil price and solid operational performance.
1st-LD-Writethru: CNOOC Ltd. posts 89.3-percent profit increase BEIJING, Aug. 27 (Xinhua) -- China National Offshore Oil Company Limited (CNOOC Ltd.) on Wednesday announced first-half net profit rose by 89.3 percent over the same period last year amid high oil prices and rising production.
Text of report by Singapore newspaper The Straits Times website on 30 July [Article by Michael Richardson: "Asia's Hunger for Oil and Natural Gas"] Rising demand for oil to churn up South China Sea ASIA'S thirst for oil and natural gas, together with a new generation of deep-water oil drilling vessels being built in China, South Korea and Singapore, combine to intensify the jockeying for control of a vast area of the South China Sea that is being disputed by six regional countries.
- To befool; deceive; balk; jilt.
- An illusion; a trick; a cheat.


text:
Vans Am Contest in Orlando, FL 21
Credit: Ryan Chadwick
I'm not sure what they're putting in the water in Florida these days, but none of these dudes are riding like amateurs! Check out a bunch of awesome riding from Nick Mahoney, Dan Nielson, Sam Bussell, Ricky Rogers, and more with a little help from Vans rider Trey Jones.
Ryan Chadwick's videos 0 of 21
21 comments
834 FLORIDABMX http://p.vitalbmx.com/photos/users/834/avatar/c50_619650028_1203626409.jpg?1293016760 http://www.vitalbmx.com/community/FLORIDABMX,834/all 10/08/06 55 2 1 7 76 1517 3
dan kills it. and mahoney's tailwhip...luc-e 270?!, damn!
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text:
Red Hot like Chilli Pepper
from Wu-Tang Clan – The Projects Lyrics on Genius
Red chili peppers are hot, so meth is saying wu is hot. This could also be a reference to Funk Rock-Rap group Red Hot Chili Peppers.
To help improve the quality of the lyrics, visit Wu-Tang Clan – The Projects Lyrics and leave a suggestion at the bottom of the page


text:
Community images
Internet Service Providers
published 13/04/2008
Very fast download speeds, brilliant technical sup...
Regular need to reboot router.
published 21/05/2007
faster, cheaper
poor support, headless chickens in call centres, p...
published 13/04/2008
fast speeds, good service
connection drops sometimes
published 21/04/2011
More expensive than their Unlimited package, rubbi...
published 19/08/2007
Speed, price, ease of set up, free McAfee suite
Dependant on your exchange being unbundled, lack o...
published 13/10/2007
A cheap broadband solution for basic Internet acce...
A very low quality Internet service (too poor for ...
published 23/05/2007
Affordable, reliable, fast
Mine drops the connection every night at roughly t...
published 07/05/2009
Only free with SkyTalk - still cheap without thoug...
published 07/05/2009
none for me
published 24/04/2009
Value for money (although you have to have SKY TV ...
Poor customer service, unreliable info given, no f...
published 23/08/2009
totally incompetent
published 30/11/2008
Reliable; no line-drops; very good router.
None at all!
published 15/04/2007
cheap if your a sky tv subsriber
customer service/ email outages/web portal
published 30/05/2008
Quick and good value
Can be quite inefficient
published 01/04/2007
Cheaper than others, 16 MBit line
unreliable connection, poor customer service


text:
Maryland Race 2014
Doug Gansler will meet with small business owners ahead of Democratic primary
Maryland Attorney General Doug Gansler is scheduled to visit with small business owners and voters in the Baltimore area as part of his final campaign stops before the Democratic primary next week.
DAYBREAK DAILY: Montgomery County looms large for Doug Gansler
No county resident ever has been elected governor of Maryland.
DAYBREAK DAILY: Brown, Mizeur make nice over pot
Two of Maryland's Democratic candidates for governor exchange cordial letters about plans to decriminalize marijuana.
DAYBREAK DAILY: Creigh Deeds details stabbing, urges action
On "60 Minutes", Virginia senator talks about his late son and makes a plea for action on mental health treatment.
RecommendedRecent Facebook Activity
Only On 7
ABC7 News at 4 Now Weekdays!ABC7 is giving you MORE news in your afternoons with an extra hour, an hour earlier! Join ABC7's Leon Harris and Alison Starling for what's trending in your community and world. Every weekday afternoon at 4pm right after back to back "Hot Bench" shows at 3pm on ABC7.


text: Hi, Julian Andres Klode wrote: > I just finished the creation of a repository on people.debian.org > that provides the packages needed to run Debian GNU/Linux on the > Toshiba AC100 notebook device. Very cool, thanks! I'm currently at DebConf with my AC100 (32GB, 3G, WLAN)and I currently have Ubuntu Natty running on it (who the fuck had the idea to ship that netbook with Android? :-), but really would like to get Debian armhf running on it. Any other AC100 owner at DebConf? Regards, Axel -- ,''`. | Axel Beckert <email@example.com>, http://people.debian.org/~abe/ : :' : | Debian Developer, ftp.ch.debian.org Admin `. `' | 1024D: F067 EA27 26B9 C3FC 1486 202E C09E 1D89 9593 0EDE `- | 4096R: 2517 B724 C5F6 CA99 5329 6E61 2FF9 CD59 6126 16B5


text: Race Issues < Prior Page Page 4 of 13 Next Page > Black or White By Juliana EriksonPosted Jan. 23, 2015Movies | Rare is the movie that can eloquently address racial divisiveness without making us feel as if we just took our daily multivitamin—healthy but flavorless. [http://www.worldmag.com/2015/01/black_or_white] A time for sober reflection By Lynde LangdonPosted Jan. 19, 2015Race Issues | Forty-six years after Martin Luther King Jr.'s death, America still struggles with racial tension and divisions [http://www.worldmag.com/2015/01/a_time_for_sober_reflection] Lecrae and two of my favorite old white guys By Marvin OlaskyPosted Jan. 15, 2015Abortion | With the Roe v. Wade anniversary one week away, I'm about to ask you to do something that I rarely do: Watch a 37-minute video. (That's about 30… [http://www.worldmag.com/2015/01/lecrae_and_two_of_my_favorite_old_white_guys] America's most surprising military victory was also a win over prejudice By Marvin OlaskyPosted Jan. 8, 2015History | Two hundred years ago today, on January 8, 1815, the Battle of New Orleans ended with dramatic casualty numbers: 71 Americans vs. 2,042 British soldiers. You… [http://www.worldmag.com/2015/01/america_s_most_surprising_military_victory_was_also_a_win_over_prejudice] A Reformed approach to racial reconciliation By Warren Cole SmithPosted Jan. 6, 2015Q&A | Anthony Bradley talks about God's sovereignty and how we can demonstrate the unity Jesus prayed for in His church [http://www.worldmag.com/2015/01/a_reformed_approach_to_racial_reconciliation] Police protests have consequences By D.C. InnesPosted Jan. 5, 2015Police | There should have been nothing remarkable in Carson Daly sporting a NYPD cap for hosting the NBC New Year's Eve coverage from Times Square. It was New York… [http://www.worldmag.com/2015/01/police_protests_have_consequences] My Forrest Gump moment By Andrée Seu PetersonPosted Jan. 5, 2015Race Issues | A woman, my granddaughter, and I recently went to see The Nutcracker in Philadelphia. On the walk to the Market-Frankfort El line we passed a shop with a T-shirt… [http://www.worldmag.com/2015/01/my_forrest_gump_moment] A year of racial strife By D.C. InnesPosted Dec. 29, 2014Race Issues | It has been a year of scandal at home and horror overseas: The Obamacare rollout debacle. Deadly neglect in the VA hospital system and cover-up. Unfolding… [http://www.worldmag.com/2014/12/a_year_of_racial_strife] A peacemaking funeral By Emily BelzPosted Dec. 27, 2014Police | Thousands gather at the funeral for NYPD Officer Rafael Ramos to hear about his faith and how it could heal New York [http://www.worldmag.com/2014/12/a_peacemaking_funeral] Grief in New York over officers' deaths By Emily BelzPosted Dec. 22, 2014Police | Murder of NYPD officers rends the city with more pain and upends the conversations around Eric Garner's death [http://www.worldmag.com/2014/12/grief_in_nyc_over_officers_deaths] 'We can't wait' By Sophia LeePosted Dec. 19, 2014Movies | Selma gives an engaging behind-the-scenes look at a powerful moment in U.S. history [http://www.worldmag.com/2014/12/we_cant_wait] Remembering Baxter Shavers By Warren Cole SmithPosted Dec. 17, 2014Police | The deaths of Michael Brown in Ferguson, Mo., and Eric Garner in New York City sparked protests around the country. These incidents raised important… [http://www.worldmag.com/2014/12/remembering_baxter_shavers] < Prior Page Page 4 of 13 Next Page >


text:
软件包: libsvg-graph-ruby-doc (0.6.1-3) [universe]
libsvg-graph-ruby-doc 的相关链接
Ubuntu 的资源:
下载源码包 libsvg-graph-ruby:
- [libsvg-graph-ruby_0.6.1-3.dsc]
- [libsvg-graph-ruby_0.6.1.orig.tar.gz]
- [libsvg-graph-ruby_0.6.1-3.diff.gz]
Please consider filing a bug or asking a question via Launchpad before contacting the maintainer directly.
Original Maintainer (usually from Debian):
- Dmitry Borodaenko
It should generally not be necessary for users to contact the original maintainer.
- 主页 [www.germane-software.com]
Pure Ruby library for generating charts in SVG format (documentation)
SVG::Graph allows to generate charts, that is, graphs where the values of one axis are not scalar. It has a very similar API to the Perl library SVG::TT::Graph, and the resulting charts also look the same. This isn't surprising, because SVG::Graph started as a loose port of SVG::TT::Graph, although the internal code no longer resembles the Perl original at all.
This package contains the documentation.


text:
PSH Performs...Peppermint Patty from "A Charlie Brown Thanksgiving"
The great Philip Seymour Hoffman takes on one of his most challenging roles. WATCH ON YOUTUBE
See Tim perform stand-up @ littlefield! WATCH ON YOUTUBE
The great Philip Seymour Hoffman takes on one of his most challenging roles. WATCH ON YOUTUBE
Daniel Day-Lewis is famous for totally immersing himself in roles, and his Oscar-winning turn as Abraham Lincoln was no exception. After last week's ceremony, Day-Lewis opened up about his process, which reportedly included the following:
◆ He and Sally Field, who portrayed Mary Todd Lincoln, addressed each other only as "Mother" and "Mr. Lincoln," as the couple did in real life.
◆ He even sent text messages in character.
◆ He built his own log cabin out of rails that he split himself.
◆ Wore a stovepipe hat for a whole year, even to bed and while showering. Made the hat himself from a real stovepipe (even though he was told that stovepipe hats were not made out of stovepipes, they were just called that because of the shape). Made the stovepipe himself, as well as the wrought-iron stove the pipe was attached to. Mined the iron ore by hand and cast it in a foundry, after learning the mining and metalwork trades.
See related video:
Wow…really? You picked me? I didn't prepare a speech, 'cause I never thought I had a chance of winning. Hey, Jack Nicholson! How's it going, Jack? I guess all I want to say is that this statue means nothing to me. I do what I do for the craft and the art and the love of it. Awards are completely meaningless, I'm sorry to tell you. I mean, a hundred years from now, no one is gonna remember who won Best Actor, or have the faintest idea who Clooney and Brad and the other nominees (sorry, I'm forgetting your names) were. OK, maybe you, Jack. In a thousand years, no one will know what a motion picture is, if the human race is even alive. In the post-global-warming wasteland, this hardware will have no value, except maybe as a weapon to club someone over the head with and steal their food.


text:
The first two steps in the capping of cellular mRNAs are catalyzed by the enzymes RNA triphosphatase and RNA guanylyltransferase. Although structural and mechanistic differences between fungal and mammalian RNA triphosphatases recommend this enzyme as a potential antifungal target, it has not been determined if RNA triphosphatase is essential for the growth of fungal species that cause human disease.
We show by classical genetic methods that the triphosphatase (Pct1) and guanylyltransferase (Pce1) components of the capping apparatus in the fission yeast Schizosaccharomyces pombe are essential for growth. We were unable to disrupt both alleles of the Candida albicans RNA triphosphatase gene CaCET1, implying that the RNA triphosphatase enzyme is also essential for growth of C. albicans, a human fungal pathogen.
Our results provide the first genetic evidence that cap synthesis is essential for growth of an organism other than Saccharomyces cerevisiae and they validate RNA triphosphatase as a target for antifungal drug discovery.
The m7GpppN cap structure is a defining feature of eukaryotic mRNA and is required for mRNA stability and efficient translation. The cap is formed by three enzymatic reactions: the 5' triphosphate end of the nascent pre-mRNA is hydrolyzed to a diphosphate by RNA triphosphatase; the diphosphate end is capped with GMP by RNA guanylyltransferase; and the GpppN cap is methylated by RNA (guanine-7-) methyltransferase [1].
Although the three capping reactions are universal in eukaryotes, there is a surprising diversity in the genetic organization of the capping enzymes as well as a complete divergence in the structure and catalytic mechanism of the RNA triphosphatase component in "lower" versus "higher" eukaryotic species [1]. Metazoans and plants have a two-component capping system consisting of a bifunctional triphosphatase-guanylyltransferase polypeptide and a separate methyltransferase polypeptide, whereas fungi contain a three-component system consisting of separate triphosphatase, guanylyltransferase, and methyltransferase gene products. The primary structures and biochemical mechanisms of the fungal and mammalian guanylyltransferases and cap methyltransferases are conserved. However, the atomic structures and catalytic mechanisms of the fungal and mammalian RNA triphosphatases are completely different [2,3]. Thus, it has been suggested that RNA triphosphatase is a promising target for antifungal drug discovery [2].
The triphosphatase (Cet1), guanylyltransferase (Ceg1), and methyltransferase (Abd1) components of the capping apparatus are essential for cell growth in the budding yeast S. cerevisiae[1,4-6]. Mutations of the RNA triphosphatase Cet1 that abrogate catalytic activity in vitro are lethal in vivo[7-9]; thus, it is reasonable to think that pharmacological inhibition of Cet1 function in vivo would impede cell growth. The key question is whether RNA triphosphatase is a valid drug target in other fungal species besides Saccharomyces cerevisiae (which is not a human pathogen) and whether a mechanism-based inhibitor of one fungal RNA triphosphatase could be expected to display broad spectrum activity against triphosphatases from other fungal species.
To address these issues, we have characterized the RNA triphosphatases of two other fungi, including the human pathogen Candida albicans and the fission yeast Schizosaccharomyces pombe[10-12]. The fungal triphosphatases, S. cerevisiae Cet1, C. albicans CaCet1 and S. pombe Pct1, belong to a new family of metal-dependent phosphohydrolases that embraces the triphosphatase components of DNA virus and protozoan mRNA capping systems [1,7,13,14]. The defining features of the metal-dependent RNA triphosphatases are two glutamate-containing motifs that are required for catalysis and comprise the metal-binding site in the crystal structure of S. cerevisiae Cet1. The yeast triphosphatase has a novel tertiary structure in which the active site is situated within a topologically closed hydrophilic tunnel composed of 8 antiparallel β strands, which are conserved in CaCet1 and Pct1 [2]. Mutational analysis of Cet1 has identified 15 individual side chains within the tunnel that are important for Cet1 function in vitro and in vivo[7-9]. Each of the 8 strands contributes at least one functional group to the active site. Mutational analysis of the Candida triphosphatase suggested strongly that the tunnel fold and the constituents of the active site are similar, if not identical, in Cet1 and CaCet1 [10].
Here we address the critical question of whether RNA triphosphatase is essential for cell growth in fungal species other than S. cerevisiae. This is not a straw-man issue, given that S. cerevisiae encodes two homologous RNA triphosphatases (Cet1 and Cth1), of which only Cet1 is essential for capping and cell viability [8,15]. We use classical genetic approaches to show that the respective genes encoding RNA triphosphatase and RNA guanylyltransferase are essential in S. pombe. Using a novel method of Enloe et al. [16] to test gene function in diploid C. albicans, we were unable to disrupt both copies of the CaCET1 gene, signifying that RNA triphosphatase is also essential in that species, a significant human pathogen. Based on these findings, and the presence of a Cet1 homolog in the Apergillus fumigatus proteome, we conclude that RNA triphosphatase is a valid target for antifungal drug development.
RNA Triphosphatase and RNA Guanylyltransferase are Essential in S. pombe
S. pombe RNA triphosphatase Pct1 is a 303-amino acid polypeptide with a homodimeric quaternary structure [12]. The pct1+ gene contains a single intron within the open reading frame [12]. S. pombe RNA guanylyltransferase Pce1 is a 402-amino acid monomeric protein [20]; there are no introns within the pce1+ gene. Although recombinant Pct1 and Pce1 enzymes have been purified and characterized biochemically, and shown to function in cap formation when expressed in S. cerevisiae[12,20,21], there have been no antecedent genetic studies of the essentiality of Pct1 or Pce1 in fission yeast. Here we constructed pct1Δ and pce1Δ plasmids containing 5' and 3' flanking genomic sequences in which the entire triphosphatase or guanylyltransferase coding sequence was deleted and replaced by the kanamycin resistance gene [17]. The pct1::kanMX and pce1::kanMX constructs were transformed separately into a diploid strain of S. pombe and chromosomal integrants containing one copy of the wild-type gene and one of pct1::kanMX or pce1::kanMX were selected on medium containing G418. Correct integration was confirmed by diagnostic PCR amplification of genomic DNA from the heterozygotes. We then sporulated the heterozygotes, dissected tetrads, and scored for spore viability and the presence of the kanMX marker. We found for both knock-outs that 20 out of 20 tetrads yielded only 2 viable spores and all of the viable haploids were G418-sensitive, i.e., none contained the pct1::kanMX or pce1::kanMX alleles. We conclude that the RNA triphosphatase and RNA guanylyltransferase genes are essential for cell growth in S. pombe.
Plasmid-based complementation of pct1Δ and pct1Δ
The pct1+ and pce1+ cDNAs were cloned separately into the S. pombe expression vector pREP41X (LEU2 ars1+) so as to place them under the control of the nmt1* promoter. We also cloned the intron-containing chromosomal pct1+ gene into the same expression vector. The plasmids were introduced into heterozygous pct1+/pct1::kanMX or pce1+/pce1::kanMX diploids. The Leu+ diploid transformants were selected and then sporulated. A random population of Leu+ haploids was tested for G418-resistance or sensitivity (Table 1). We found that half of the Leu+ haploids derived from a pct1+/pct1::kanMX strains containing a plasmid with either the pct1+ cDNA or pct1+ gene (with intron) also contained the pct1::kanMX chromosomal allele and were resistant to G418. Similarly, half of the Leu+ haploids derived from a pce+/pce1::kanMX strain containing the pce1+ plasmid were resistant to G418. In contrast, none of the Leu+ haploids derived from pct1+/pct1::kanMX or pce1+/pce1::kanMX strains containing the control LEU2 plasmid vector lacking an insert were G418-resistant. These results show that the pct1Δ and pce1Δ strains are viable if the chromosomal deletions are complemented by an extrachromosomal triphosphatase or guanylyltransferase gene. There was no apparent difference in complementation of pct1Δ by the intron-containing pct1+ gene versus the pct1+ cDNA.
Table 1. Plasmid-based Complementation of pct1Δ and pce1Δ
Although the plasmid-encoded capping enzyme genes are under the control of a regulated nmt1* promoter, which can be repressed by inclusion of 5 μg/ml thiamine in the growth medium [19], we observed that the growth of the plasmid-dependent strains was not affected by exogenous thiamine. We suspect that expression levels of the Pct1 or Pce1 enzymes in these strains exceeded a threshold required for cell viability.
Test of CaCET1 Essentiality in C. albicans
Candida albicans strains are diploid and do not undergo meiotic division. Thus, the classical approach of allelic disruption in diploid cells followed by sporulation and segregation analysis of haploids is not applicable to the analysis of gene function in C. albicans. Tests of gene essentiality in Candida necessitate serial disruption of both alleles using two different selection markers. If the gene of interest is nonessential, a homozygous diploid disruptant can be isolated. However, if the gene is essential, it will be impossible to disrupt both alleles. Mitchell and colleagues [16] have developed a single-transformation method to test gene function in diploid C. albicans that entails the following steps, which we have applied to the CaCET1 gene encoding RNA triphosphatase [22].
First we constructed a deletion allele plasmid containing 5' and 3' genomic sequence flanking the target CaCET1 gene and an intervening marker cassette (ura3Δ3'-ARG4-ura3Δ5', referred to as UAU1) composed of the C. albicans ARG4 gene flanked by overlapping 5' and 3' fragments of the URA3 gene. This construct deletes the coding sequence for amino acids 206 to 506 of the 520-aa CaCet1 polypeptide. The deleted segment contains the catalytic domain essential for triphosphatase activity in vitro and for complementation of the cet1Δ strain of S. cerevisiae[10,11]. Second, we introduced the linearized deletion allele into a diploid C. albicans ura3/ura3 arg4/arg4 strain and selected for Arg+ transformants. Correct insertion via homologous recombination into one copy of the CaCET1 gene, resulting in cacet1::UAU1 (Figure 1), was confirmed by Southern blotting of genomic DNA digested with diagnostic restriction endonucleases. For example, a probe specific for the 5' end of the CaCET1 gene (probe A in Figure 1) hybridized to a single 4.4 kbp BglII fragment after restriction digestion of total DNA from the parental diploid strain, whereas the heterozygote contained an additional 2.7-kbp fragment derived from scission at a novel BglII site located within the ARG4 component of the UAU1 insert of the disrupted cacet1::UAU1 allele (Figure 2A, lane P versus lane H). The 2.7-kbp fragment was also detected with an ARG4-specific probe (not shown). We found that the heterozygous CaCET1/cacet1::UAU1 strain displayed normal growth and morphology (not shown).
Figure 1. Genotype of the CaCET1/cacet1::UAU1 heterozygote strain of C. albicans. Illustrated in cartoon form are the configurations of the wild-type CaCET1 and the cacet1::UAU1 chromosomal loci in the Arg+ heterozygous diploids. The positions of pertinent restriction sites and the CaCET1 5'-specific (A) and 3'-specific (B) hybridization probes are shown. Also shown is the configuration of the triplicated cacet1::URA2 allele in the Arg+ Ura+ segregants.
Figure 2. Southern blot analysis. DNA isolated from the parental diploid strain (lane P), one of the Arg+ heterozygotes (isolate #19; lane H), and fourteen independent Arg+ Ura+ segregants were digested with BglII and resolved by agarose gel electrophoresis. A photograph of the ethidium bromide-stained gel is shown in panel C. The positions and sizes (kbp) of DNA size markers are indicated on the right. The DNA was transferred to a Hybond membrane, which was serially hybridized to 32P-labeled DNA probes A (panel A) and B (panel B) derived from the 5' and 3' segments of the CaCET1 gene, respectively.
Third, we grew 54 independent liquid cultures of the heterozygotes in nonselective medium and then selected for cells that were Arg+ and Ura+. Uracil prototrophy requires restitution of the integrity of the disrupted ura3 gene of the UAU1 cassette by recombination between the overlapping regions of the ura3Δ3' and ura3Δ5' fragments with excision of the intervening ARG4 gene [16]. If CaCET1 were nonessential, then recombination of UAU1 into the second copy of CaCET1 (to generate cacet1::UAU1/cacet1::UAU1) followed by excisional recombination of ARG4 in one allele to restore URA3 (generating cacet1::UAU1/cacet1::URA3) would result in the selected Arg+ Ura+ phenotype with complete loss of the wild-type CaCET1 locus. However, if CaCET1 is essential for growth, then all of the Arg+ Ura+ isolates will have three copies of the CaCET1 locus (cacet1::UAU1/cacet1:: URA3/CaCET1).
We used Southern blotting to determine the genotype of one randomly selected Arg+ Ura+ derivative from each of the 54 separate cultures of the heterozygote diploids. The blots were probed with a 5' specific CaCET1 fragment (probe A in Figure 1), which detects both the wild-type CaCET1 allele and the cacet1::UAU1 allele, and with a 3'-specific CaCET1 fragment (probe B in Figure 1) derived from the segment deleted during construction of the cacet1::UAU1 disruption cassette. Note that probe A hybridized to a single 4.4-kbp BglII fragment in both the parental diploid strain and the heterozygote (Figure 2B, lanes P and H), thereby verifying that it did not detect the disrupted allele. We found that 54/54 Arg+ Ura+ isolates retained the wild-type CaCET1 locus (Figure 2 and data not shown), implying that CaCET1 is an essential gene. All 54 isolates also retained the cacet1::UAU1 allele that was present in the heterozygote (Fig. 2A and data not shown) and they acquired a new ~5-kbp BglII fragment that hybridized to 5'-specific CaCET1 probe (Figure 2A and data not shown). The novel BglII fragment migrated identically in 53/54 of the strains analyzed. Recombination within UAU1 to regenerate URA3 eliminates the BglII site and results in a cacet1::URA3 locus that would yield an ~5-kbp fragment upon digestion with BglII (Figure 1). Therefore, we surmise that the vast majority of the events leading to the Arg+ Ura+ phenotype entailed allelic triplications. This conclusion is supported by additional Southern analyses of ScaI digests and EcoRI/PstI digests of genomic DNA from the parental diploid, the heterozygotes, and the 54 Arg+ Ura+ segregants (data not shown).
We conducted in parallel an analysis of the function of the C. albicans CES1/ZDS1 gene, which encodes a protein homologous to the product of the S. cerevisiae CES1/ZDS1 gene isolated by us and others in various suppressor screens [[23] and references therein]. We found that 3/26 Arg+ Ura+ segregants emanating from a CES1/ces1::UAU1 heterozygote were homozygous for disruption at both loci (ces1::UAU1/ces1::URA3) and had lost the wild-type CES1/ZDS1 allele [B. Schwer, unpublished]. This frequency of homozygosity at a nonessential locus is similar to that reported by Mitchell's group (2 out of 30) for homozygous disruption of the C. albicans CDC25 gene [16]. These results confirm that the single-transformation test can, in our hands, be used to identify a nonessential gene and they underscore the inference from the data presented here that RNA triphosphatase is essential for growth of C. albicans.
Previous genetic analyses establishing the essentiality of cap formation were performed in the budding yeast S. cerevisiae. It remained to be seen whether the homologs from other fungal species are also essential for viability. It is not a foregone conclusion that essentiality or dispensability of a gene product in S. cerevisiae can be extrapolated to pathogenic fungi. For example, Cdc25 is not essential in C. albicans whereas its homolog is essential in S. cerevisiae[16]. Conversely, the enzyme DNA topoisomerase I is nonessential in S. cerevisiae and S. pombe, but essential for viability in the pathogenic fungus Cryptococcus neoformans[24].
Here we have shown that the RNA triphosphatases Pct1 and CaCet1 are essential for viability of S. pombe and C. albicans, respectively The conclusion that CaCet1 is essential is based on a finding that none of the 54 independent isolates in the single-transformation test were homozygous for cacet1Δ; our interpretation is consistent with criteria established by Mitchell and colleagues for inference of essentiality using this genetic approach. De Backer et al. [25] had previously generated a single allele knockout in C. albicans of the guanylyltransferase component of the capping apparatus (Cgt1) using the URA-blaster technique and noted a variety of pleiotrophic effects on stress response, hygromycin sensitivity, and colony morphology in the CGT1/cgt1Δ heterozygote, but they found that the heterozygote was just as virulent as the wild-type strain in animal models of systemic candidiasis. They were unable to recover a homozygous cgt1Δ/cgt1Δ isolate after a second transformation with the URA3 disruption cassette after testing 13 transformants. Although their sample size was not large, their data, together with the present findings, indicate that the triphosphatase and guanylyltransferase are both essential for viability of C. albicans.
RNA triphosphatase is an attractive therapeutic target for fungal infections because: (i) the active site structure and catalytic mechanism of fungal RNA triphosphatase are completely different from the RNA triphosphatase domain of the metazoan capping enzyme and (ii) metazoans encode no identifiable homologs of the fungal RNA triphosphatases. Thus, a mechanism-based inhibitor of fungal RNA triphosphatase should be highly selective for the fungal pathogen and have minimal effect on the human or animal host. This scenario is plausible only if RNA triphosphatase is essential for growth of pathogenic fungi that cause human disease (e.g., Candida albicans, Aspergillus fumigatus, Cryptococcus neoformans, Pneumocystis carinii, etc.). The finding that the RNA triphosphatase CaCet1 is essential in the pathogenic fungus C. albicans provides impetus for the discovery of compounds that inhibit CaCet1 activity. Searches of public genome databases indicate that Aspergillus fumigatus (a major invasive pathogen in humans, with severe morbidity and mortality) encodes a homolog of Cet1, as does Neurospora crassa. Thus, we suspect that all fungal species will have metal-dependent RNA triphosphatases resembling those of S. cerevisiae, C. albicans and S. pombe.
Methods and materials
Gene disruption in S. pombe
We used a modified version of the long flanking homology PCR technique [17] to produce pct1Δ and pce1Δ gene disruption cassettes in which the open reading frames were replaced by the kanMX gene. For each gene, a set of four primers was synthesized: LI, a 20-mer corresponding to the sense-strand sequence of the 5'-flanking region ~1.2 kb upstream of the translation start codon of pct1+ or pce1+; L2, a 40-mer in which 20 bases were identical to the 5' sequence of pFA6a-KanMX4 (GCTTCAGCTGGCGGCCGCGT) and 20 bases were identical to the antisense strand sequence immediately 5' of the translation start site of pct1+ or pce1+; L3, a 40-mer in which 20 bases were identical to the 3' sequence of pFA6a-KanMX4 (AGTGGCCTATGCGGCCGCGG) and 20 bases corresponded to the sense-strand sequence immediately 3' of the stop codon of pct1+ or pce1+; L4, a 20-mer corresponding to the antisense-strand sequence of the 3'-flanking region ~1 kb downstream of stop codon of pct1+ or pce1+. In the first-stage PCR, a 5'-flanking fragment was synthesized using S. pombe genomic DNA as the template and LI plus L2 as primers. The 3' flanking fragment was synthesized using primers L3 and L4. In the second-stage PCR, aliquots of the purified products from the first amplification (0.1–0.2 μg) were mixed with 0.5 μg of NotI-digested pFA6a-kanMX4 and amplification synthesis was primed with the LI and L4 oligonucleotides. The products of the second PCR amplification were gel-purified and subcloned into pGEM-T (Promega). The recombinants were selected on LB agar medium containing 100 μg/ml ampicillin and 60 μg/ml kanamycin. The pPCT1Δ and pPCE1Δ plasmid constructs were confirmed by restriction enzyme digestion and partial sequencing. The pct1::kanMX cassette was PCR-amplified from the pPCT1Δ plasmid using primers LI and L4. The pce1::kanMX cassette was excised from PCE1Δ by digestion with AatII and NdeI. The cassette fragments were gel-purified and then used to transform diploid S. pombe.
The S. pombe diploid strain was generated by crossing two heterothallic strains FY527(ura4-D18 leuI-32 ade6-M216 his3-DI h-) and FY528(ura4-D18 leuI-32 ade6-M210 his3-D1 h+) on ME plates at room temperature. After 24 h, the cells were streaked onto medium lacking adenine to select for diploids. The Ade+ diploids were verified by staining with phloxin B and a single diploid colony was picked and incubated in 100 ml of YE medium to prepare competent S. pombe cells. The transformations were performed using the lithium acetate method [18]. The integrants were selected at 30°C on YE plates containing 200 μg/ml G418. Single colonies were restreaked on YE agar containing G418. Genomic DNA was prepared from individual isolates and the integration of the pct1::kanMX or pce1::kanMX cassettes into the correct locus was tested by PCR using diagnostic primers. The heterozygous diploids were sporulated on ME plates at room temperature. Tetrads were dissected from single asci and the spores were incubated at 30°C. All viable haploids were tested for growth on YES agar and YES agar containing 200 μg/ml G418.
S. pombe expression vectors for RNA triphosphatase and guanylyltransferase
The cDNA encoding Pct1 was amplified from plasmid pET-PCT1 [12] using primers that introduced an XhoI site immediately upstream of the translation start codon and a BamHI site immediately downstream of the stop codon. The intron-containing chromosomal pct1+ gene was amplified from total S. pombe genomic DNA. The intron-less pce1+ gene was amplified from plasmid pl32-PCE1 [12]. The PCR products were digested with XhoI and BamHI and then inserted into the S. pombe expression vector pREP41X (LEU2 ars1+) [19]. The inserts were sequenced to exclude the acquisition of unwanted mutations during the amplification and cloning steps. Expression of the capping enzymes from these plasmids is driven by the nmt1* promoter [19]. The plasmids were transformed into heterozygous pct1+/pct1::kanMX or pce1+/pct1::kanMX diploids using the lithium acetate method [18]. The Leu+ diploid transformants were then sporulated on ME plates at room temperature. A loopful of cells was inoculated into 500 μl of sterile water and the mixture was incubated overnight at 28°C with 10 μl of β-glucuronidase (Sigma G7770). The spores were plated on EMM(-Leu) agar medium and incubated at 30°C. Individual colonies were then restreaked onto YES agar and on YES agar containing 200 μg/ml G418. Growth was scored after incubation for 5 to 7 days at 30°C.
Gene disruption in C. albicans
The CaCET1 gene was disrupted by insertion of a UAU1 cassette [16]. We first constructed plasmid pKS-5'3'CaCET1, in which a 665-bp PCR fragment derived from the 5' end of the CaCET1 gene (from nucleotides -50 to +615 of the open reading frame, with the A residue of the ATG translation start codon defined as position +1) was cloned between the KpnI and XbaI sites of pBluescript KS+ and a 720-bp fragment extending from position +1518 of the 1560-nt CaCET1 coding sequence into the 3' flanking genomic region was inserted between the SacI and SacII sites of pBluescript KS+ The 3.8-kbp UAU1 gene was excised from pBME101 with XbaI and SacII and inserted between the XbaI and SacII sites of pKS-5'3'CaCET1 to yield pCaCET1::UAU1. This DNA was linearized with KpnI and SacI and then transformed into the diploid C. albicans strain BWP17 using the lithium acetate method. We selected 25 Arg+ transformants and analyzed them by Southern blotting for integration of the UAU1 cassette into one of the two CaCET1 chromosomal loci to yield the heterozygote CaCET1/cacet1::UAU1 configuration depicted in Figure 1. Briefly, genomic DNA was isolated from the 25 Arg+ strains, then digested with ScaI (which cuts neither CaCET1 nor UAU1). The digests were resolved by agarose gel-electrophoresis and transferred to membranes, which were probed with a radiolabeled DNA corresponding to the 5' segment of CaCET1 (probe A in Figure 1). Whereas probe A hybridized to a single 3.8-kbp ScaI fragment in the parental BWP17 strain, the probe detected two fragments in the heterozygote – a 3.8-kbp fragment corresponding to the wild-type CaCET1 locus and an ~7.5-kbp fragment corresponding to cacet1::UAU1 (data not shown). This analysis identified 16/25 of the Arg+ transformants as CaCET1/cacet1::UAU1 heterozygotes. Recombination rates at the UAU1 gene in the heterozygote were determined as described by Enloe et al [16]. Ura+ segregants arose at a rate of 5 x 10-5 per division and Arg+ Ura+ segregants arose at rate of 8 x 10-9 per division.
The sixteen CaCET1/cacet1::UAU1 heterozygotes were streaked to YPD agar and grown for 3 days at 30°C. A total of 54 single colonies derived from the 16 heterozygotes were inoculated into separate YPD liquid cultures. After growth to saturation, aliquots of the cultures were plated on SD(-Arg-Ura) agar medium. Genomic DNA was prepared from one Arg+ Ura+ segregant from each culture and subjected to restriction digestion and Southern analysis.
We thank Dr. Aaron Mitchell for plasmid pBME101 and C. albicans strain BWP17. This work was supported by NIH grant GM52470 to SS and BS.
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La Barrosa Table Set
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Description: La Barrosa Table Set:
- Outdoor seating set nests into a complete circle
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BEER-SHEVA, ISRAEL, June 29, 2010 - The U.S. Food & Drug Administration (FDA) has granted Investigational New Drug (IND) regulatory clearance to initiate a Phase I/II clinical trial evaluating Alpha-1 Antitrypsin (AAT) in type 1 diabetics, based on research by Dr. Eli Lewis of Israel's Ben-Gurion University of the Negev.
This is the first time AAT will be evaluated in humans with type 1 diabetes. AAT is an FDA-approved off-patent drug currently used to treat pulmonary emphysema among youngsters and adults with an AAT genetic deficiency.
"We designed the trial with the same dose of AAT that has been used safely and effectively for over 20 years," explains Dr. Lewis, who is the director of Israel's only Clinical Islet Laboratory and a senior lecturer in the Department of Clinical Biochemistry at BGU in Beer-Sheva, Israel.
Dr. Lewis received a generous five-year Young Scientist Career Development Award from the Juvenile Diabetes Research Foundation (JDRF). He has also received the prestigious Yigal Alon Scholarship, the Wolf Foundation Krill Award, a Marc Rich Foundation award and two Israel Science Foundation grants.
Dr. Lewis conducted and published research studies using animal models to demonstrate that AAT may be effective in reversing type 1 diabetes during his post-doctoral fellowship at the University of Colorado and later in his own lab at BGU. He determined that eliminating inflammation is the key to pancreatic islet beta cell survival and to restoring normal glucose levels.
This approach could potentially eliminate the need for daily insulin shots in recently diagnosed individuals, whose native circulating AAT molecules appear to be inactivated by high glucose. AAT is also potentially potent following pancreatic islet transplantation, sparing patients from intense immunosuppressive therapy. The procedure of islet transplantation is the only known approach today that affords continuous physiological glucos
|Contact: Andrew Lavin
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"I have no problem with that," he added. "We've been waiting a long time for that."
And the winger also explained how he eagerly wants to compete for playing time with them — as long as he gets what he considers a fair shot to keep a sizable role.
"My point is, if they're better than I am, I am expecting and accepting a smaller role," Selanne said. "But if I'm better, I need to play more. I need to play top minutes. That's what I'm looking for.
"That's fair for everybody. To be honest, I've been playing a big role. I don't know if I'm ready to play 10-12 minutes a game. And that's what I want to make sure. If I play well, I get what I deserve.
"Sometimes it's not like that. So I want to make sure I'm treated the way I deserve."
The quietly hovering question is whether Selanne, the greatest and most popular player in franchise history, is in the way of the team getting younger. Asked if he feels he is wanted by the Ducks, Selanne said he believes so in his discussions with Murray.
"He's always very clear, very honest," Selanne said. "He said, 'Teemu, I'm more than happy to see you back. It's your decision. If you want to play, let's do it.
"I have a lot of respect for Bob and the way how they have been treating me. I haven't talked with Bruce (again) so I don't really know his approach. But Bob? It's a no-brainer for him."
The Ducks will open training camp Sept. 11. Murray has left $4.3 million under the salary cap to fit Selanne in and the winger said he doesn't see any problem reaching a deal if he comes back.
There are a number of motivations for Selanne to do one last turn through the NHL.
He believes the Ducks are better than the team that went out in the first round. The club will also play in their first outdoor game, against the Kings at Dodger Stadium. And he can be the first hockey player to have participated in six Winter Olympics.
It is well apparent Selanne wants to play for the Ducks — as long as they make the likely future Hall of Famer feel like he is a needed asset.
"Like I said five years ago, I don't have to play," he said. "But I've been enjoying playing. I want to make sure I can continue enjoying it as much as I have in the past. That's the biggest thing for me.
"If everybody's on the same page with that, then it's a green light. Let's go."
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French Montana ft. Baby, Wale, Fabolous & Jadakiss - Everythings A Go (Remix)
Artist: French Montana
Featuring: Baby aka Birdman, Wale, Fabolous, Jadakiss
Producer: Prime
Album: Excuse My French
Avg Rating: 2.2 ( 10 votes )
Over the past few months, French Montana has been going crazy on the feature tip, contributing guest rhymes to cuts by everyone from Rick Ross to MGK. As it turns out, however, there was method to his madness. Today, the rhymesayer cashes in a few of those favors, assembling an all-star feature cast for the official remix of Everythings A Go, the current single off his Bad Boy debut set. Prime's whip-ready instrumental, a Lex Luger-esque blend of orchestral loops and trap percussion, is held over from the original along with the hook, but all rhymes are brand new. In addition to a fresh 16 by Montana himself, the record features added mic work by YMCMB mogul Baby, Maybach Music heavyweight Wale, Big Apple mainstay Fabolous and D-Block veteran Jadakiss. Who turns in the dopest performance? Since all five emcees sound right at home stunting over the record's street-ready backdrop, I'm going to have to let y'all break the tie. Whatever your take on the matter, you'll be able to find the album version of this record and much more on Excuse My French, tentatively scheduled to arrive on store shelves this July.
French MontanaBaby aka BirdmanWaleFabolousJadakiss Hitmaker
Written by richard on May 10, 2012
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Cheap LAP to Gabes flights
Popular Flights from La Paz, Baja California Sur
Top La Paz Flights to US Destinations
Top La Paz International Destinations
Popular Flights from Gabes, Tunisia
Top Gabes Flights to US Destinations
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LAP to GAE Route Map
La Paz to Gabes Flights, Stats and Airplanes
Route information for flights from Manuel Marquez de Leon Intl. to Gabes.
6,842.05 miles
LAP - Manuel Marquez de Leon Intl. Airlines
Browse the most popular airlines servicing the La Paz (LAP) airport. Visit their flights pages to learn more about the most popular routes these carriers fly.


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SLIDELL, La. - The 12-year-old girl whose body was found after a boating accident died from blunt force trauma to her head, according to the St. Tammany Parish Coroner's Office.
Samantha Vinturella died instantly after the trauma, said a spokesman for the coroner. The coroner, Dr. Peter Galvan, said the death was not from drowning.
Vinturella struck the windshield of the boat before entering the water.
Herbody was found Tuesday in Lake Borgne, about 5-6 miles from the accident scene.
Vinturella was on a boat driven by Paul Battle, 48,of Mandeville. He could face charges because the young girl was not wearing a life jacket in his 15-foot Logic vessel, a violation of state law.
According to the Louisiana Department of Wildlife and Fisheries, the three other young girls in Battle's boat were reportedly wearing life jackets. They were also thrown from the vessel in the collision, but were rescued by a passing boat.
According to officials, Battle's boat suffered minor damage, but the other boat, a 20-foot Kenner, had significant damage.The driver of that vessel has been identified as Marion Funchess, 61, of Folsom.
Officials said the two crashed head on as Battle was headed in from the bayou, and Funchess was headed out.


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8 posts tagged with cheeseburger.
Displaying 1 through 8 of 8. Subscribe:
Colonel Sanders, tear down this wall.
The fast food arms race continues to spiral out of control as Carl's Jr introduces a foot-long cheeseburger. [more inside]
Burgers burgers burgers
The perfect burger starts with the perfect blend of beef. [more inside]
Cheese and Burger Society
Goodnight, Ricky. Goodnight, Julian. Goodnight, Bubbles.
[NSFW] It's almost time to Say Goodnight to the Bad Guys -- the final episode of the Trailer Park Boys aired in December, 2008. (As Bubbles says, "It's a dirty, sassy liquor. So sassy.") Producer Mike Clattenburg says that there will be a second movie, "Countdown to Liquor Day", to be released late in 2009. After that, though, the TPB franchise will buy the great double-wide in the sky. [pervyously, preevisilly or however th' fuck you say it.] [more inside]
DO NOT WANT unless starving
I can has... wait, it's canned? Canned cheeseburger, only €3.95. [more inside]
Illuminati with Cheese.
I Want to Believe--in the Double-Double Animal Style. Mefi has discussed the wonders of In-N-Out Burger before, but what fascinates this east-coaster is the "Secret Menu." Also, the cryptic biblical messages. In-N-Out HQ laughs it all off, even as its greasy-spoon archipelago falls apart, potentially.
How many times was the word
How many times was the word "cheeseburger" spoken in the first Olympia Restaurant sketch? Everything you always wanted to know about NBC's Saturday Night Live but were afraid to ask. The answer is "80," by the way.
11-year-old files $1M suit against McDonald's for maggot-infested cheeseburger.
11-year-old files $1M suit against McDonald's for maggot-infested cheeseburger. This is in the same league as the woman who got a fried chicken head in her McNuggets a while ago.
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- Company improves margins to offset higher material prices.
- Cash position increases by $1 million in the year.
ST. LOUIS, Sept. 7 /PRNewswire-FirstCall/ -- Allied Healthcare Products, Inc. (Nasdaq: AHPI) reported that its net income increased 38 percent to $869,000, or 11 cents per share, during its fourth quarter ending June 30, 2007, versus $629,000, or 8 cents per share, for the fourth quarter last year.
Despite its stronger fourth quarter, Allied experienced flat net income of $1,641,600, or 21 cents per share, compared to $1,648,600, or 21 cents per share, for the prior fiscal year.
Fourth quarter sales decreased by about 3 percent, or $417,000, compared to the last quarter of the prior year. However, lower costs and expenses more than offset the sales decline, producing a solid increase in net income despite income tax expenses for the fourth quarter of 2007 that were $606,000 higher than for the fourth quarter of 2006. The 2006 fourth quarter benefited from a settlement and resolution of state tax contingencies.
For fiscal year 2007, Allied sales declined about $1.04 million, or 2 percent, compared to 2006. Lower costs for 2007 offset the decline.
Higher prices for raw materials such as copper, brass and zinc increased costs about 5.4 percent in fiscal 2006. Despite competitive pricing pressures, Allied was able to selectively increase prices during 2007. These increases, combined with programs to cut costs, resulted in improved margins for Allied.
"We are far from satisfied with flat results for 2007," said Earl
Refsland, president and chief executive officer. Refsland said the company
would continue to hold the line on costs in 2008 and work to increas
|SOURCE Allied Healthcare Products, Inc.
Copyright©2007 PR Newswire.
All rights reserved


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|Property type
|Minimum Stay
|2 nights
1 Bedroom apartment, sleeps 3. 250m to beach, 2km from pretty fishing village of Makryiaglos, S. East Crete. Balcony at rear with seating area, table & chairs. Lots of fitted wardrobe space. Apartment has air conditioning, dishwasher, TV, DVD, fridge-freezer, cooker, microwave etc, all new, with solar glazed windows & mosquito nets, providing all that you need for a relaxing holiday or romantic break. We also have a sofa bed available.
The Bay View Resort is very attractive with swimming pools & other facilities, creating a friendly, community feel. There is car parking & BBQ area to the rear of the apartment & all around are landscaped gardens with seating areas & a stone amphitheatre, with beautiful views of the sea & mountains. Makryiaglos means 'Long Beach' in Greek, the blue flag beach stretches for several kms past a small marina, tavernas, restaurants, shops, hotels, gym and spa.
What is there to do in the local area?: Dependent upon your preferences, you can relax by the pool or on the roof garden, or take the short walk down to the beach. You can walk or drive to Makryiaglos and try out their 'long beach' or visit one of the many small restaurants along the shoreline or near the fishing port. There are a few lively bars and Tavernas in Makryiaglos in the evening, or you may chose to visit one of the many restaurants all within a few minutes of Bay View. Nearby towns of Sitia and Ierapetra can be reached within 30 mins and have shopping and nightlife to suit most tastes. The hills and mountains are beautiful and great for walking, cycling or climbing. You can go fishing, sailing, snorkelling, jet-skiing or play golf on the island, or just drive around exploring the many villages, towns, ancient ruins or beaches. You can take a ferry from Ierapetra to the beautiful unspoilt Chrissi Island, which is a tropical paradise. The Mikri Polis Hotel in the town has a gym, spa and restaurants available to use.
Bayview Resort Crete is registered under number 1040K123K3018001 at the Greek National Organisation of Tourism
The apartment was excellent, everything is brand new and clean inside. Outside was lovely too, well maintained gardens, large pool area which considerably quiet, wasn't ever more than 10 people around the pool.
It is in walking distance to the local town however we only tried it once but it the heat and carrying shopping we couldn't really manage it so ended up hiring a moped from one of the local car rentals which was only €70 for the week, so not too bad.
Overall it was a lovely holiday, very relaxing and quiet, with lots of local activities to get involved in.
Would defiantly go again! Many thanks.
Wir haben in diesem Apartment im Juli eine wunderschöne Urlaubswoche verbracht und wollten am liebsten dort bleiben!
Die Beschreibung entspricht absolut der Wirklichkeit. Die Wohnanlage ist traumhaft angelegt und man hat vom riesigen Balkon aus einen tollen Blick auf das Meer. Das Apartment ist absolut neuwertig und modern ausgestattet und lässt keine Wünsche offen. Bei unserer Ankunft war es tip top gereinigt, besser als in jedem Hotel.
Auch die Klimaanlage hat uns sehr gute Dienste geleistet, sie kühlt wirklich sehr schnell und zuverlässig.
Das Bett ist extrem bequem, die Küche ist perfekt ausgestattet (es gibt sogar eine Spülmaschine).
Man ist mit dem Auto in 5min im nächsten Ort und hat dort eine große Auswahl an Restaurants und Supermärkten. Der nächste Strand ist zu Fuß nur 5min entfernt, wir waren aber lieber am feinen Sandstrand von Makrygialos (ca. 10min mit dem Auto).
Die Rezeptionistin Anja hatte immer ein offenes Ohr bezüglich allen Fragen und war sehr hilfsbereit. Alles vor Ort war perfekt organisiert.
Wir können das Apartment absolut empfehlen und werden garantiert wieder kommen!
we were in the Bay View Resort for a week in june the place is all new two kind people from holland start this project 6 years ago (i think) now is near to finish , that man work hard for improve the resort ...for example when i was here he takes 10 canarian palm and put it on the side of the resort for the shadow.
my baby 6 years old matteo play a lot with mike the son of that man in the swimming pool , all the pool are amazing the apartment is really new all is perfect, the air condition work well and the bed are long and comfortable.
the only mistake is the cover of the window because the sun arrive inside the bedroom very early, but is not so important.
the place is perfect to visit the est of crete ....from here you can visit in an hour by car : the town of sitia , vai beach with the forest of palm, chirokambi beach and zakros...if you rent that resort don't miss a dinner at " the dragons cave" a nice restorant near the resort .
at the end the resort is very nice , cheeper ,clean,and not too busy.....you can grill drink with friends and the people who work inside are so kind and friendly....if you want to visit that uncontaminated part of the island the resort is perfect .
16M is a one bedroom apartment on the new Bay View Resort just outside the small fishing village of Makrygialos. The village has shops, restaurants and tavernas and a delightful marina, which is in regular use with the locals and tourists alike. The apartment has superb views and is just a short 250 m walk to the sandy beach. The rooms are fully furnished with modern appliances and necessary air-conditioning, with a balcony at the front overlooking the nearest swimming pool. The area of Makrygialos is tranquil and authentic and whilst there are plenty of bars and tavernas, this is not the place for wild partygoers! The surrounding area is beautiful for walks, mountain-biking, hiking, fishing or rock climbing. Views are breathtaking all around this part of Crete and deserted beaches are easily found with delightful places to swim in the shallow warm sea.
|Rental Period
|Minimum Price
|Additional Nights
|Weekend Night
|Event Price
Base Rate
2 night minimum stay
for 2 nights
1 May - 30 Jun
2 night minimum stay
for 2 nights
|Discounts: 14 nights or more: £25 Nightly
1 Jul - 31 Aug
2 night minimum stay
for 2 nights
1 Sep - 31 Oct
2 night minimum stay
for 2 nights
|Discounts: 14 nights or more: £25 Nightly
1 Nov - 30 Apr
2 night minimum stay
for 2 nights
|Discounts: 14 nights or more: £15 Nightly
Please Note: The owner of this property accepts payment in the original currency specified (GBP), before use of conversion tool. The conversion tool is provided for your convenience only. The owner may accept other currencies if specifically agreed in advance.


text: Original owner and kept like new. Factory upgraded High Output 385 HP Crusaders. Risers recently replaced. Bottom soda blased, barrier coated due to owners requirements to keep as new. Enclosure and bimini top only 3 years old. Aprox 300 hours on upgraded 7.5 Gen set. Top line electronics. MUST SEE to Appreciate this beauty.


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Indian investigators drop coal scam case against billionaire Birla
Published on Aug 30, 2014 2:10 PM
NEW DELHI (REUTERS) - India's Central Bureau of Investigation (CBI) has closed a coal scam case against billionaire Kumar Mangalam Birla and a former top bureaucrat that emerged in 2012 after an auditor's report on revenue loss to the exchequer from allocations of coal blocks.
The CBI filed the case against Birla and former Coal Secretary P.C. Parakh last year in relation to a block allocated in 2005 to Hindalco Industries, part of the US$40 billion (S$49.93 billion) Aditya Birla Group led by Birla.
"The evidence collected during investigation did not substantiate the allegations levelled against the persons named" in the First Information Report filed in the case, the CBI said in a statement late on Friday.
India's federal auditor had alleged that the government's allocation of coal blocks may have cost the exchequer revenues of about US$33 billion, although industry watchers and the previous government have cast doubts on the figure.
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At seven o'clock on the morning of April 14, 1865, President Lincoln came out of his bedroom, nodded to the night guard, and started down the hall to his office. At 7:22 the following morning Surgeon General Barnes pressed silver coins to his eyelids.
The president's day was, as usual, crammed with meetings and appointments, but he was probably no busier than John Wilkes Booth, the man who would stand behind him at Ford's Theatre that evening. Although the plan had been long in the making, the time and place were not set until eleven that morning.
The Day Lincoln Was Shot chronicles the movements of these two men minute by minute until the almost unbearable suspense is shattered with a single gunshot and a leap to the stage. From a thousand bits and scraps of information, Jim Bishop has fashioned an unforgettable tale of tragedy, more gripping than fiction, more alive than any newspaper account.


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Stuck in Zacatecas, Mexico
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Joined: Feb 2003
Location: Atlanta, GA
Oddometer: 4,560
Originally Posted by
I'm not sure if I'm posting this request for feed back in the best spot, as any help will be appreciated. I have an '09 DR 650. It began acting up about a week ago...all of the issues I'm about to list happened inconsistently...hard starting (needed to hold throttle open to start), would shut off at idle, hesitation at varying RPM's, and finally it wouldn't start at all.
A tech at the Suzuki dealer in Zacatecas took the carburetor apart, inspected and cleaned it. It still didn't start. He installed new plugs (old plugs looked like it was running rich) and checked for spark (good) and it still won't start. Install new gas, same.
The tech also checked the timing and it was correct.
When trying to start it, in order to hear anything encouraging from the engine, the throttle must be open. It tries but won't start.
Question...could it be a faulty CDI, a coil, a bad ground, or something else? Feedback from anyone with DR 650 experience will be appreciated.
Finally, I'd prefer resolve my problem here and to continue my trip, but will ship the bike home if I have no other option.
Could have solved it day one if you would have added. "I recently added a new aftermarket stator."
It is common in trouble shooting to check everything that was recently done on the item whether you think it was done perfectly or not. New parts are defective too.
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- Joe Breeze, Motorsports
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KANSAS CITY, Kan. -- The wild card for Sunday's Sprint Cup Series race at Kansas Speedway?
Heat. There'll be plenty of it.
Soaring temperatures and relative humidity promise to make the inaugural STP 400 one slick proposition.
Will tempers flare too?
We'll find out soon enough.
Five more things to watch:
1. Is Carl still king? -- Few drivers have been hotter than Carl Edwards, who'd been working on a string of five straight top-10 finishes -- not including a victory in the All-Star Race -- before recording a pedestrian 16th at Charlotte last Sunday.
Edwards is the Sprint Cup points leader and carries a ton of momentum, but the pride of Columbia, Mo., has never won a Cup race at his home track.
How hungry is he to get it done Sunday?
"I told Bob [Osborne, crew chief] this week that this race is really important to me; I mean, all of them are, but to win here in the Cup series would be huge," Edwards told reporters Friday. "We have what seems to be a really fast race car, and practice went really well. It is going to be hot and slick, which should be fun. I am just glad to be here racing ..."
Edwards does have one trip to Victory Lane at Kansas. He won a Truck series race here in 2004, and it was memorable.
"I was racing too hard on the first lap and smacked the fence and then came back and won the race," Edwards recalled. "It was a very special win."
Oddly, Clint Bowyer, who hails from nearby Emporia, Kan., hasn't won a Cup race here, either, though he did lead 124 of 167 laps in winning Saturday's O'Reilly Auto Parts 250.
"It would be big," Bowyer said this week of a possible Kansas win. "Once it [Kansas track] was built, I drove by it every week and would set goals just to be able to drive there in anything. I never dreamed I would be able to drive an ARCA car there, let alone start a race in a Sprint Cup Series car."
Edwards qualified seventh Saturday, Bowyer 27th.
2. Roush rolling -- Edwards isn't the lone Roush Fenway Racing driver with a strong chance to find Victory Lane on Sunday.
Greg Biffle has two wins here, including a victory in the 2010 fall race. He also leads the Cup series at this track in laps led (323) and average finish (8.1).
Biffle had one of the most dominant cars in last week's Coca-Cola 600, but he had to stop for gas before the final restart and settled for 13th.
The Biff's secret at Kansas?
"If I told you, I would have to kill you," he said Friday. "You know, I really like this racetrack. It is a lot of fun. It being hot and slick is kind of my favorite. It is hard to get ahold of this and it is different on both ends.
"This place you can run up the racetrack, which is fun to do. You can run right up against the fence. This place has a lot of grip in the center of it, too. ... It has a little slower corner speeds and you have to be a little technical, which has played into my hands over the years, I guess."
RFR teammate Matt Kenseth is no slouch, either. He led 103 laps in the Coke 600 before fuel issues relegated him to a 14th-place finish. Even David Ragan joined in on the fun, finishing a career-best second behind surprise winner Kevin Harvick.
3. Junior overdue? -- Dale Earnhardt Jr. had the Charlotte race all but won before his No. 88 Chevy ran out of fuel entering Turn 3 of the final lap -- another heartbreaking near miss for NASCAR's most popular driver.
Afterward, Earnhardt appeared satisfied with seventh place and maintaining his fourth-place hold in the Cup standings.
When you run good,
it's much easier to want to show up.
"-- Dale Earnhardt Jr.
In Sunday's race at Kansas, where he has four top-10s in 10 starts, Earnhardt knows he will have his hands full. But he also knows his 105-race winless streak won't last forever.
"I think that we came close to winning that race and we've done it a couple times this year, and that makes me feel more confident in us having that opportunity again more often throughout the season," said Earnhardt, who qualified 28th. "I feel like that if we just keep doing that, then the luck is going to fall our way and we'll get to Victory Lane one way or another."
Regardless, Earnhardt said he enjoys race weekends more than he has over the past couple of seasons.
"Preparing for the weekend -- I look forward to it a little bit more than I did in the past for sure," he said. "When you run good, it's much easier to want to show up."
4. Can Gordon right the ship? -- Jeff Gordon christened Kansas Speedway by winning the track's first two races in 2001 and 2002. His stats here are impeccable: In 10 races he has seven top-5s -- including the two wins -- and eight top-10s.
Gordon's numbers through 12 races this season aren't nearly as impressive. He has one win and three top-10s but over the past five races he has finished no better than 12th.
Still, he's only 15 points shy of the final Chase spot. And the one victory he scored at Phoenix gives him a leg up in the wild-card race.
"It is not just about being in the top 10 in points, it is about being a threat for the championship ..." Gordon said behind his hauler Saturday. "I think, from a points standpoint right now, what we have to focus on is winning races because, if we can win another one or two races, to me that locks us in [the Chase].
"We understand that not only locks us in, it gives us momentum to be in a position to actually be a threat for the championship."
5. Petty Blue -- Some old-timers will watch this race just to get a glimpse of the Richard Petty Motorsports No. 43 Ford Fusion.
The car on Sunday will feature the same paint scheme made famous by The King during his glory years of the '70s, a palette of Petty Blue and STP Day-Glo Red.
Granted, it's not a Dodge. And Petty won't be turning the wheel. But the car will be in the capable hands of AJ Allmendinger, who's got some momentum after a sixth-place run at Charlotte.
No pressure, right?
"I wake up every day and feel very fortunate to get into the 43 and have such a huge fan base when it comes to the number and the history of NASCAR," Allmendinger said Friday. "It comes full circle to be driving the iconic 43, and now I have a paint scheme that especially all the old-school fans looked at and loved as they grew up and watched The King. For me, I feel very fortunate to be a part of [it].
"I'm excited, and thank STP for coming back and being a part of this and allowing me to be their driver."
Naturally, Petty, the owner of seven Cup championships and the winner of a record 200 races, will serve as grand marshal.
STP last served as a primary sponsor on the 43 car for two races in 2006.
Joe Breeze is a motorsports editor at ESPN.com. He can be reached at email@example.com
Breeze: Five things to watch at Kansas
How badly does Carl Edwards want to win at Kansas? Can Jeff Gordon right the ship? Just how cool is AJ's new ride? We break down Sunday's STP 400.


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American Congress of Obstetricians and Gynecologists
American Pregnancy Association
Health Canada
The Society of Obstetricians and Gynaecologists of Canada
American Congress of Obstetricians and Gynecologists. Ultrasound in pregnancy.
Practice Bulletin
High amniotic fluid levels: polyhydramnios. American Pregnancy Association website. Available at:
http://americanpregnancy.org/pregnancycomplications/highamnioticfluidpolyhydramnios.htm. Accessed January 2007. Accessed June 10, 2013.
Polyhydramnios. March of Dimes website. Available at:
http://www.marchofdimes.com/pregnancy/pregnancy-complications.aspx#InDepthTabLong%5F1887. Updated June 2011. Accessed June 10, 2013.
Prenatal ultrasound screening. EBSCO DynaMed website. Available at:
http://dynamed.ebscohost.com/about/about-us. Updated January 23, 2013. Accessed June 10, 2013.
This content is reviewed regularly and is updated when new and relevant evidence is made available. This information is neither intended nor implied to be a substitute for professional medical advice. Always seek the advice of your physician or other qualified health provider prior to starting any new treatment or with questions regarding a medical condition.
Copyright ©
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I need advice ..off shore or Intracoastal NJ to Florida
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Re: I need advice ..off shore or Intracoastal NJ to Florida
Hello sir ..
Yes , defiantly some logistical issues .. The big picture is/was to keep the boat and base out of the Florida area ... I was thinking to have the mast stepped and rigged in Florida as well .. and if I was to keep the boat at the Johnson yard or dockside I was hoping that I could be able to stay aboard to save the motel costs ..
My call to the Johnson yard tomorrow may give me a idea if they even have dock space available and how much $ ?? I am not big daddy Warbucks so I need to consider all the costs . And I totally agree that December is not a idea time to start a trip down unknown to myself waters in a untested sailboat with not sails .
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Drivers Beware of Wet Weather
As Midpoint of Winter,
"Ninety percent of driving decisions are based on clear vision," says
Clear visibility through the use of effective wiper blades and a windshield treatment while driving can improve reaction times by up to a full second in poor weather conditions. When traveling at 60mph, a full second can equate to 100 feet and may be the difference in avoiding a potential accident.(3)
To raise awareness around the issues of clear vision and safe driving, Rain-X is teaming up with
- Conduct a regular walk around your vehicle, checking its lights, antifreeze, windshield washer fluid and wiper blades regularly
- Ensure that your tires are properly inflated and have sufficient tread
- As the temperature drops, keep your gas tank at least half full; the extra gas helps reduce condensation that can plug your fuel line with ice and stall your engine in cooler weather
- Create an emergency pack of items such as a blanket, a first aid kit, snacks, bottled water, an ice scraper, a flashlight and a bag of kitty litter, and keep it in your vehicle. Such items can be critical during roadside emergencies in heavy rain or snow storms.
"In addition to keeping these emergency items in the vehicle, drivers should adjust their driving habits in inclement weather," continues DeVere. "Drivers should slow down when the roadways are wet, leave extra distance between your vehicle and the one in front of you, and be sure to use signals when changing lanes to indicate your intentions. Utilizing these quick and easy safety tips can help keep everyone safer on the roads."
For additional information on automotive safety and wiper blades, please visit www.askpatty.com or www.rainx.com.
About AskPatty.com:
The Ask Patty.com, Inc. website and blog is a safe place for women to get advice on car purchases, maintenance and other automotive related topics. Women can shop for vehicles at our network of certified female friendly dealerships across the U.S.
AskPatty.com is a member and 2008 corporate sponsor of the Women's Automotive Association International based in
AskPatty.com is partnered with AutoTrader.com, NIADA, DriverTv.com, MyCarPage.com, MyAutoLoan, Aware and BlogHer.com.
About Rain-X:
Rain-X products are produced and marketed by Shell Lubricants. The term 'Shell Lubricants' collectively refers to the Shell Group companies that are engaged in the lubricants business. Shell lubricants companies are global leaders in lubricants and operate in approximately 120 countries worldwide. They manufacture and blend products for use in a range of applications from consumer motoring to food processing and heavy industry to commercial transport. The Shell portfolio of top quality lubricant brands includes Pennzoil(R), Quaker State(R), FormulaShell(R), Shell Tellus(R), Shell Cassida(R), Shell ROTELLA(R) T, Shell Spirax(R) and a portfolio of car care products and Jiffy Lube(R) services.
(1) www.sciencedaily.com - Bad Weather Drivers, 06/08 (2) Survey of licensed drivers 18 years and older, October 2007. (3) AAA
SOURCE Rain-X


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Narrow your search
The search giant has a low-cost contraption made of cardboard that can turn any smartphone into a virtual reality headset. The goal: mass appeal.
GamSpot's Rob Handlery and Mary Kish try out some of the hottest GTA 5 Mods, including the Bodyguard Menu, Police Partner and Riot mode. Some may have worked better than others.
Technically Incorrect: It isn't every day you see a humpback whale. You won't see one at all if you've always got your nose in your phone.
For the first time, researchers have used a video camera mounted on a hexacopter to monitor the behaviour of killer whales in the wild.
Want to know what your next phone's operating system will look like, how Google wants to manage all your photos or how you'll pay with your Android phone? Look here.
Vine users everywhere are breaching the surface and singing whale songs to feed the weird new #whaling movement.
Researchers enlist a satellite in the quest to take a count of endangered southern right whales. Eerie pictures result.
The man responsible for Voyager's "golden record" wants to beam crowdsourced data to the New Horizons spacecraft and take it beyond our solar system.
A researcher from the National Oceanic and Atmospheric Administration finds a most unusual shark in a most unusual place.
A team of researchers in England has discovered how a worm with no mouth or stomach may have messed with the ocean's fossil record for millions of years.


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02 April 2012 14:27 [Source: ICIS news]
LONDON (ICIS)--Cytec Industries has acquired the manufacturing assets of India-based Star Orechem International Private Limited (SOIL) in N?xml:namespace>
Cytec said the acquisition will increase the global capacity for its metal extraction product line by approximately 25%.
"This acquisition enables us to meet the strong growth we are seeing for our extractant products coming from increased use of this technology in both new copper mining projects as well as existing properties," said Shane Fleming, chairman, president and CEO of Cytec.
The acquired operations will manufacture Cytec's ACORGA solvent extractant products and will also continue to supply solvent extractant products for SOIL's distribution operations and supply obligations, the group said.
($1 = €0.75)
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text:
BEIRUT: Round two of a campaign to "topple the sectarian regime" will take place Sunday with a march in Beirut, as open-ended sit-ins have now begun in both the capital and Sidon for the same objective.
The demonstrators will gather in Dora at 3 p.m. and proceed to the Electricite du Liban building in the neighborhood of Nahr in Beirut.
Prior to the march, a conference will be held at UNESCO Palace to coordinate activities for the campaign, which has begun to spark interest around the country.
While activists who support an end to the sectarian system are expected to arrive Sunday from Tripoli, Jbeil, Nabatieh, Sidon, and elsewhere, a group of demonstrators will launch their protest action early Sunday morning in Sofar, Aley and a string of villages in Mount Lebanon – they intend to travel on foot to Beirut, in the hope of encouraging the public to join in.
The first protest action by the anti-sectarian campaign took place last Sunday, when hundreds of people turned out in a driving rainstorm for a march in Beirut.
The campaign has been promoted by a number of Facebook groups, all centered on the goal of toppling the sectarian regime and its "leading figures."
The groups stress their total independence from political parties, most of which appear to be lukewarm about the campaign.
According to Nehmat Badredinne, the campaign's spokesperson, "we are people who differ from each other, and we might find ourselves [suited to joining] this or that [Facebook] group, but all of us aim to topple the sectarian regime and leave our differences aside," she added.
One of the activists noted that the campaign is trying to leave the realm of Facebook and concentrate efforts on the logistics of organizing demonstrations.
"Division won't infiltrate into the movement, no matter how many groups on Facebook there are, we are one group now on the ground," said Rawad Shami. "Committees are being created all over the country in order to simplify communication and the mobilization [needed for] demonstrations. We're trying to have less activity and news on Facebook; we need more privacy."
A public appeal was issued late Thursday, stressing the peaceful nature of the protest and calling on the entire public to support the effort.
"For those who ask who we are, we are Lebanese citizens, and we question this sectarian regime and its cruelty," the statement said.
The statement enumerated a series of objectives, such as a civil personal status law, social justice, and sound proper public education.
Organizers have been cautious about being seen as receiving support from traditional political leaders and groups.
Ali Dirani, an activist, said "sectarian political leaders are trying to take advantage of the movement and adopt it, under their principles, but we have rejected such acts."
"If they believe that [our cause] is just, let them resign from their parties and they will be more than welcomed with us as ordinary members," he said.
"They have invited us into meetings but we refused to attend these meetings, we want to topple the sectarian regime, along with its figures," Dirani said.
Organizers say that a number of political parties are anxious about the campaign, and are adopting various strategies to fight back.
Dirani said that a week ago in Beirut, two people distributing fliers in Beirut were kidnapped for five hours and roughed up, before eventually being released.
Meanwhile, enthusiastic supporters erected tents in a public square in Sidon and held a noisy rally Friday to generate support for the campaign.
They painted the slogan "abolishing sectarianism" at the roundabout next to the sit-in, and chanted slogans such as "we're not afraid of the leaders of sects."
A number of passers-by joined the protest, which highlighted deteriorating socio-economic conditions as a major grievance.
Malak Khalil, one of those who joined the protest after it began, said "we have the hope that change will take place. We're not sectarian – what brings us together is our desire for changing the sectarian system, to arrive at social justice."
In Beirut, activists began an open sit-in Friday at Sanayeh garden near the Interior Ministry, where organizers say the protests will feature cultural and intellectual activities.
Organizers said the two sit-ins were not part of the original action plan, but were entirely spontaneous, representing enthusiasm by people enthusiastic about the goal of ending sectarianism.
A website (http://isqatalnizam.org) was recently created to publish the movement's activities and appeals. The website includes print and video media coverage of the protest actions and information about future events.
"We ask people to join us on Sunday at 3 p.m. in Dora to first topple the sectarian regime and its figures, and second to liberate ourselves from a system that has manipulated our lives for more than three decades," Dirani said. – with additional reporting by Mohammed Zaatari
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