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In the past few years, both automated and manual high-throughput protein expression and purification has become an accessible means to rapidly screen and produce soluble proteins for structural and functional studies. However, many of the commercial vectors encoding different solubility tags require different cloning and purification steps for each vector, considerably slowing down expression screening. We have developed a set of E. coli expression vectors with different solubility tags that allow for parallel cloning from a single PCR product and can be purified using the same protocol.
The set of E. coli expression vectors, encode for either a hexa-histidine tag or the three most commonly used solubility tags (GST, MBP, NusA) and all with an N-terminal hexa-histidine sequence. The result is two-fold: the His-tag facilitates purification by immobilised metal affinity chromatography, whilst the fusion domains act primarily as solubility aids during expression, in addition to providing an optional purification step. We have also incorporated a TEV recognition sequence following the solubility tag domain, which allows for highly specific cleavage (using TEV protease) of the fusion protein to yield native protein. These vectors are also designed for ligation-independent cloning and they possess a high-level expressing T7 promoter, which is suitable for auto-induction. To validate our vector system, we have cloned four different genes and also one gene into all four vectors and used small-scale expression and purification techniques. We demonstrate that the vectors are capable of high levels of expression and that efficient screening of new proteins can be readily achieved at the laboratory level.
The result is a set of four rationally designed vectors, which can be used for streamlined cloning, expression and purification of target proteins in the laboratory and have the potential for being adaptable to a high-throughput screening.
The establishment of rapid expression and purification procedures for recombinant proteins has become a major challenge for biotechnology and indeed any laboratory which studies proteins. The genomic and structural genomic communities have driven the development of high-throughput cloning, expression and purification technologies to a large extent. Recent developments including filter-plate based assays for cloning, expression and purification, ligation-independent cloning (LIC) [1], Gateway technology [2] and auto-induction for protein expression [3] are now readily coupled to robotic pipelines that have made the parallel production of proteins a relatively simple, cost-effective approach. E. coli remains the system of first-choice for expressing proteins, as it is cheap and easy to handle, however many mammalian proteins cannot be successfully expressed in E. coli [4]. This leaves the researcher to either explore expression space using a range of alternative E. coli strains, different temperatures, solubility tags or choose an alternative expression host [5,6].
There are numerous commercial and non-commercial E. coli expression vectors available that incorporate fusion tags (for both purification and enhanced solubility), Gateway or LIC technologies, protease cleavage sequences and regulable expression levels [7]. These vectors are generally designed to be used in diverse expression/purification applications and thus often contain extraneous sequences at either the N- or C-termini of the protein. In addition, the use of different fusion tags mean that alternative purification protocols are required for each protein expressed from a different vector, increasing the time it takes to find successful expression and purification conditions. As a result of these limitations, we set out to create a set of E. coli expression vectors that incorporate these essential features: i) protein expressed from each vector is initially purified in the same manner; ii) the cloning procedure is the same for all vectors and iii) a range of solubility tags are available. Moreover these vectors have been developed for use at the general laboratory level so that a researcher can rapidly screen a range of expression conditions.
Here, we describe a set of four rationally designed expression vectors for E. coli. The first, (pLIC-His) encodes for a hexa-His tag followed by a TEV recognition sequence. The other three encode for three common solubility tags [MBP [8], NusA [9], GST [10] plus a TEV recognition sequence. TEV protease was selected due its superior specificity and adaptability to a range of buffering conditions [11]. The vectors are LIC compatible, and contain an N-terminal hexa-His tag enabling parallel cloning and purification, expression is via a T7 promoter allowing for auto-induction and they all possess a TEV recognition site between the fusion partner and the gene of interest. These features make the vectors ideally suitable for high-throughput cloning and expression screening.
We demonstrate that soluble expression was observed for a range of proteins and that they could be readily digested with TEV to yield the native protein. In addition, we illustrate the relevance of parallel cloning in deciding on a solubility tag that is suitable for a target protein. This demonstrates that the vectors can be used successfully at the laboratory level to rapidly screen target proteins (or their mutants) and can be readily adapted to the high-throughput process.
Results and discussion
Construction of the pLIC vectors and His-HHR23A, GST-UIM, MBP-α1-AT and Nus-UBL
Several vector systems have been described that incorporate a variety of backbones, promoters, tag positions and cloning procedures. One drawback from several of these studies is the lack of consistency within the vector systems which does not allow a systematic study of protein expression [12-15]. Thus the aim of this study was to generate a set of vectors which enable parallel cloning of a given target(s) and enables a comparative analysis of the expression yields using different solubility tags. As a result, the pLIC set of prokaryotic vectors were created using pET21b(+) as their host. An oligonucleotide cassette was used to introduce the His tag, TEV site and LIC site as well as two unique restriction sites (NcoI-SpeI). These unique sites were used to introduce either the genes encoding for the GST, MBP or Nus tags and could in principle, be used to introduce alternative tags. The four vectors, termed pLIC-His, pLIC-GST, pLIC-MBP and pLIC-Nus were created as shown in Figure 1. It is to our knowledge, the first set of E. coli expression vectors derived from the same backbone, with the only variation being in the solubility tag present. The genes HHR23A, UIM, α1-AT and UBL were subsequently amplified and successfully introduced into each of the vectors using LIC. In addition, HHR23A was cloned into all vectors simultaneously to monitor the effect that different tags had on the solubility of this particular protein.
Figure 1. Basic expression vector design. (A) The basic vector design for the pLIC vectors. (B) Shows the nucleotide sequence for the features common to all the vectors. The pLIC-His vector is identical in sequence except it does not contain the NcoI-SpeI restriction enzyme sites between the His tag and the TEV recognition sequence.
Two recently developed ligation methods (Gateway technology (Invitrogen) and LIC) have been used extensively by the structural genomics community. Both techniques offer the opportunity for parallel cloning, such that multiple targets can be easily cloned into several different vectors. With the LIC strategy in particular, target genes are amplified with 12–15 bp end sequences and the 5'-3' endonuclease activity of T4 DNA polymerase is then exploited [1,16] (Figure 2). This digestion event generates overhangs in the PCR product that allows subsequent insertion into a complementarily digested vector using a simple annealing step. As there is no need for restriction enzymes or ligation steps, LIC is a highly efficient and cost effective means of cloning. It also has the advantage that it does not require expensive reagents or multiple manipulations.
Figure 2. Ligation-Independent Cloning (LIC) Methodology. Shows the general procedure for LIC (1) The primer sequences required for amplification of the gene of interest. (2) Both the vector and insert are subject to treatment with T4 DNA polymerase, in the presence of either dTTP (vector) or dATP (insert). (3) The result after successful annealing. (4) After cleavage with TEV protease, the tags are removed and the protein retains 4 amino acids (GAAS).
The LIC technique was found to be an efficient process in our hands, whereby up to 80% of the colonies screened were found to be positive. We observed, however, that success in LIC lies in the quality of the linearized vector, which was dependent, in part, on using a vast excess of SacII enzyme. Gel purification was used in the preparation of the vector and insert and it serves three purposes: by removing unwanted (and possibly active) DNA polymerase, removing excess dNTPs and selecting the correct PCR product over other misprimed events or linearized vector over undigested material. In a high-throughput format, a sizeable quantity of PCR product would be generated (and can be verified by sequencing) prior to parallel cloning into the vectors.
Expression and purification of the constructs
Each of the constructs was transformed into the E. coli host BL21(DE3) and expressed in 2 ml of auto-induction expression media. Following expression, the cell density of each of the cultures were normalised to an OD600 nm of 5.0 prior to lysis, which allowed for a uniform number of cells between each sample. The cultures were chemically lysed using PopCulture (Novagen), which is a chemical method for lysis and particularly useful for multiple samples. After lysis, the cell lysates were subjected to a solubility assay (as described in the Materials and Methods) to assess the relative proportions of soluble and insoluble expression. As shown in Figure 3A, all of the constructs were found to have soluble expression, as judged by presence of a respective bands at the predicted molecular weights. Both His-HHR23A and MBP-α1-AT have marginally greater proportions of insoluble material, whilst Nus-UBL and GST-UIM have slightly elevated proportions of soluble material. Optimization of soluble yields was not performed in this study, as this can vary greatly from protein to protein. It has been recently been shown however, that a lower temperature used during auto-induction may improve the yield of soluble material [3]. The soluble expression and purification of MBP-αl-AT was particular interesting, as αl-AT is generally produced in very high yields in an insoluble form and purified using a refolding method [17,18].
Figure 3. Small scale expression, purification and cleavage of recombinant proteins. (A) SDS-PAGE analysis of the total cell lysate (T), insoluble (I), soluble (S), and the purified (P) fractions for each of the fusions: His-HHR23a, GST-UIM, MBP-α1-AT and Nus-UBL (the protein is marked with an asterisk). (B) SDS-PAGE analysis of each fusion during TEV protease digestion, (1) intact fusion (2) TEV digestion (3) purified protein after digestion. The band around 30 kDa observed in lane 2 of each experiment corresponds to the TEV protein.
In addition to screening different targets in each of the vectors, we also cloned a single gene (HHR23A) into all four vectors. As can be seen in Figure 4, HHR23A expressed largely as inclusion bodies, however soluble material was obtained in all four vectors. More importantly however, is that the tags had differing effects on the expression of HHR23A. In this instance, GST was found to be the most efficient in improving the expression whereas NusA had little additional benefit (Figure 4). This illustrates therefore, the usefulness of parallel cloning and expression, as a protein may express more favourably with a different tag.
Figure 4. Parallel expression, purification and cleavage of HHR23A. HHR23A was cloned into all 4 vectors and the constructs were expressed and purified as described in the materials and methods. SDS-PAGE analysis of the total cell lysate (T), insoluble (I), soluble (S), and the purified (P) fractions for each of the fusions are shown.
An additional observation of the small-scale expression was the importance for a purification step (Ni-NTA). In some cases, soluble expression of the protein showed a very faint band in the soluble fraction, but was purified at more detectable quantities (see α1-AT band, Figure 3A). This however, appears to be dependent on the degree of soluble expression, as the UBL and UIM constructs for instance, showed very clear results in the solubility assay (Figure 3A). The use of the solubility assay in isolation should thus be used with caution, particularly for a poorly expressing protein that may produce unclear results. In some cases, it may be useful to complement the assay with a Western blot.
TEV cleavage
Generally, affinity tags are removed after purification as they can interfere with either the protein's function or with downstream processes. The most common example is if the protein is to be used for crystallographic or NMR studies. While there have been reports of proteins being crystallized with the assistance of an affinity tag [19-21] the general view is that they should be removed [22]. Each of the constructs described above has a TEV cleavage site which can be used to remove the affinity tags, leaving the native protein with 4 vector-derived residues (GAAS) (Figure 1). This is comparable to another LIC vector available which describes 3 vector-derived residues (S-N-A) [14]. Indeed the introduction of non-native amino acids at the N- terminus is not ideal and it is a common problem that generally exists both for the LIC and Gateway technologies. As a result, the effect of such residues (both in number and composition) on the expression, purification and integrity of a given protein must be verified experimentally [12].
To validate the TEV cleavage site, each of the proteins were digested with his-tagged TEV protease and purified. As seen in Figure 3B, after cleavage three species are present: the solubility tag (26 kDa GST, 42 kDa MBP, 54 kDa NusA), the his-tagged TEV protease (30 kDa) and the protein of interest. This mixture can be purified further to remove the his-tagged TEV protease and the solubility tag by selectively binding them to a Ni-NTA column. The protein of interest, on the other hand remains unbound to the Ni resin as it lacks a His tag (Figure 3B).
It is should be noted that although a given protein target may express solubly, its behaviour in the absence of the solubility tag may be very different, as this is a function of other factors (structure, amino acid sequence, aggregation potential, buffering conditions). Thus the integrity of the protein after TEV cleavage should be verified using other methods (dynamic light scattering, size exclusion chromatography, circular dichroism etc.).
Here, we have presented a set of four rationally designed T7-based E. coli expression vectors whose features include the incorporation of solubility tags to assist in expression/purification, a TEV cleavable sequence and a LIC sequence. We have shown soluble expression and purification of four different proteins as well the ability to remove the tags using TEV protease. These vectors allow for rapid parallel cloning and expression and are suitable for either laboratory-scale screening of target proteins, or alternatively for high-throughput screening.
Construction of pLIC-HIS and pLIC-MGN
The vector pET21b(+) (Novagen) was used as the basis for the pLIC vectors. pET21b(+) was manipulated to produce two different constructs, pLIC-His and pLIC-MGN. For the pLIC-HIS construct, the vector's unique NdeI-BamHI restriction sites were used. An oligonucleotide cassette containing the His-tag, TEV and LIC sites was created and ligated into the vector which had been previously digested with NdeI-BamHI (Figure 1). The cassette was comprised of the following oligonucleotides: 5'-tatgcaccatcaccatcaccatgaaaacctgtatttccagggagcagccgcggccggtgctttgcag-3' and 3'-acgtggtagtggtagtggtacttttggacataaaggtccctcgtcggcgccggccacgaaacgtcctag-5'. pLIC-MGN was created by utilising the same NdeI-BamHI sites, but incorporating a cassette of the following oligonucleotides: 5'-tatgcaccatcaccatcaccatggaactagtgaaaacctgtatttccagggag cagccgcggccggtgctttgcag-3' and 3'-acgtggtagtggtagtggtaccttgatcacttttggacataaagg tccctcgtcggcgccggccacgaaacgtcctag-3'. This cassette encodes the His tag, a unique NcoI site, a unique SpeI site, TEV recognition sequence and the LIC site (Figure 1). It was also ligated into pET21b(+).
Construction of pLIC-GST, pLIC-MBP and pLIC-Nus
The introduction of the unique NcoI and SpeI site in pLIC-MGN allowed for the introduction of the solubility tags: GST, MBP and NusA. The GST gene was cloned from the vector pET41 (Novagen), the MBP gene from pMAL-2cx (New England Biolabs) and NusA from pET43.1 (Novagen). The oligonucleotides used for the amplification are presented in Additional file 1. As the NusA gene already contained a NcoI site, the QuikChange site-directed mutagenesis technique (Stratagene) was used to remove this site, using the vendor's instructions. Briefly, the plasmid was amplified using a pair of complementary primers (See Additional file 1) and using the polymerase Pfu. The PCR product was then digested with 10 u of DpnI, followed by transformation into competent DH5α E. coli.
The genes encoding GST, MBP and NusA were amplified and each of the PCR products were subjected to sequential digestion with NcoI and SpeI followed by gel purification and ligation into the pLIC-MGN vector. This yielded three different constructs termed: pLIC-GST, pLIC-MBP and pLIC-Nus.
Cloning of UIM, α1-AT, HHR23A and UBL into the vectors
To clone into the expression vectors, PCR products of the gene of interest need to be generated with specific overhangs that are complementary to the sequence of the vector (Figure 2). After cleavage with TEV, the native protein will have four vector-derived residues (G-A-A-S). To validate the vectors, four genes were chosen to be expressed: HHR23A was cloned into pLIC-His, ubiquitin interacting motif (UIM) into pLIC-GST, α1-antitrypsin (α1-AT) in pLIC-MBP and ubiquitin-like domain from HHR23A (UBL) into pLIC-NUS.
The LIC site consists of a unique SacII site, which can be used to linearize the vector and thus permit T4 DNA polymerase treatment to take place (Figure 2). 30 units of SacII (New England Biolabs) was used to digest 1 μg of the target vector in a total reaction volume of 50 μl for a total of 3 hours. The linearized vector was then separated and gel purified from an agarose gel and the DNA concentration determined using UV-spectroscopy. 0.2 pmol of DNA was then treated with T4 DNA Polymerase (1 u/0.1 pmol DNA) and 2.5 mM dTTP at 22°C for 30 minutes followed by heat inactivation for 20 minutes.
Each of the four genes was amplified using Pfu polymerase (Promega) using the oligonucleotides detailed in Additional file 1. The amplification was for 30 cycles with the following conditions: 95°C 1 min, X°C 1 min, 72°C for Ymins. The annealing temperatures (X) and extension times (Y) for each of the constructs are shown in Additional file 1. After amplification, the PCR products were gel purified prior to being treated with T4 DNA Polymerase (1 u/0.1 pmol) and 2.5 mM dATP at 22°C for 30 mins, followed by a 20 min heat inactivation step.
For the annealing process, 1 μl of T4 treated vector was mixed with 2 μl of T4 treated PCR product and allowed to incubate at 22°C for 1 hour. 1 μl of 25 mM EDTA pH 8.0 was then added and the reaction incubated for 5 mins. The annealed mix was then transformed into competent JM107 E. coli. The colonies were assessed by colony PCR and the clones were verified using DNA sequencing.
Additional File 1. Oligonucleotide sequences used to isolate the genes for GST, MBP and Nus, as well as the annealing and extension times used for amplification.
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Expression and purification of His-HHR23A, GST-UIM, MBP-α1AT and Nus-UBL
Each of the clones were transformed into competent BL21(DE3) E. coli cells and streaked onto plates. A single colony was selected and expressed in 2 ml of Overnight Express Media (Novagen) for 18 hours at 30°C, 300 rpm. The OD600 nm was recorded for cells after growth (typically ranging between 8–11) and the absorbance was normalised to OD600 nm = 5.0 for each, which allowed for a uniform number of cells between samples. The cells were then lysed with 0.2 ml of PopCulture supplemented with 2 μl of lysozyme and benzoase, as per manufacturer's recommendation (Novagen). 200 μl of each cell lysate was retained for the Solubility Assay (see below). The remaining material was bound batch wise, to 200 μl of Ni-NTA resin which had been previously equilibrated in buffer (25 mM NaPO4, 500 mM NaCl pH 8.0). The slurry was then collected in a 2 ml filter plate (supplied with the solubility assay kit, Novagen) and washed with 4 ml of buffer supplemented with 25 mM imidazole. The proteins were then eluted with 250 mM imidazole in 25 mM NaPO4, 50 mM NaCl pH 8.0 in a 200 μl volume.
Solubility assay
The solubility assay (Novagen) was used to determine the relative proportions of soluble and insoluble material that were expressed for each of the constructs. After lysis with PopCulture, 200 μl samples of each lysate was passed through the wells of a 0.2 μm filter plate (supplied by the manufacturer) and collected. Here, the soluble protein passes through the plate whilst the insoluble inclusion bodies are retained. 200 μl of 4%(w/v) SDS was then added to each well and following a 10 min incubation the insoluble proteins eluted. A sample of both the soluble and insoluble fractions were retained for SDS-PAGE analysis.
TEV protease cleavage
The concentration of protein was estimated using the BCA Assay (Pierce). Protein at a concentration of 0.5 mg/ml was incubated with 0.05 mg/ml of TEV and allowed to incubate at 4°C for 16 hours. The sample was then run on a SDS gel to check for the completion of cleavage
Authors' contributions
LC carried out the cloning and expression studies, participated in the design of the study and drafted the manuscript. WD participated in the molecular biology. SB conceived of the study, and participated in its design and coordination and helped to draft the manuscript. All authors read and approved the final manuscript.
The authors would like to thank members of the lab for useful discussions and also to Peter Howley for providing the plasmid containing the HHR23A gene. S.P.B is a NH&MRC R.D. Wright Fellow and a Monash University Logan Senior Research Fellow. L.D.C is a NH&MRC CJ Martin Fellow.
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Health News Archive - May 11, 2009
As the childhood obesity epidemic in the United States continues, researchers are examining whether early parent and child behaviors contribute to the problem.
HUBBARD, Ohio, May 11 /PRNewswire-FirstCall/ -- NanoLogix, Inc. (Pink Sheets: NNLX) is pleased to announce the creation of its Science Advisory Board to help evaluate and target research and development efforts for products.
AYER, Mass., May 11 /PRNewswire/ -- Never again will cream pie be off limits to the 50 million Americans with lactose intolerance, thanks to Nasoya(R) Silken Creations, the first-ever non-dairy dessert starter. "Silken Creations has all the sweet, creamy taste and texture of traditional pie-making ingredients like milk, cream and butter, but without any of the lactose.
NEW YORK, May 11 /PRNewswire-USNewswire/ -- The National Center on Addiction and Substance Abuse (CASA) at Columbia University commends the National Association of Boards of Pharmacy(R) (NABP(R)) for its report State of the Internet: NABP Position Paper on the Continued Proliferation of Rogue Internet Drug Outlets.
Menarini Group chooses Enterprise Quality Management Solution to manage quality and maintain regulatory compliance HOLMDEL, N.J., May 11 /PRNewswire/ -- Sparta Systems, Inc., the maker of TrackWise(R) software and the market leader in enterprise quality and compliance management, today announced that Menarini Group, the world's leading Italian pharmaceutical group, has selected its TrackWise Enterprise Quality Management System (EQMS) to simplify quality processes throughout distributed manufacturing sites.
ATLANTA, May 11 /PRNewswire/ -- AtlantaNAP (www.atlantanap.com), a premier co-location facility, today announced its newest client, Piedmont Healthcare. As part of continual operational excellence planning and to support future growth, Piedmont will use AtlantaNAP to provide a primary data center facility for its healthcare operations.
New 'ReadyPA' Materials Offer Tips on Creating Personal Support Networks HARRISBURG, Pa., May 11 /PRNewswire-USNewswire/ -- Older adults and individuals with disabilities can be better prepared for emergencies with the help of new resources offered by Pennsylvania's ReadyPA campaign, the Department of Health announced today. "The ReadyPA campaign encourages all Pennsylvanians to be informed, be prepared and be involved," said state Health Secretary Everette James.
DETROIT, May 11 /PRNewswire/ --Blue Cross Blue Shield of Michigan today announced it plans to offer its customer groups the ability to purchase coverage for autism treatment programs that provide intensive early intervention.
BURLINGTON, Mass., May 11 /PRNewswire-FirstCall/ -- Palomar Medical Technologies, Inc.
Foster Farms maintains commitment to 100% Natural and launches new consumer awareness campaign exploring economic and health impact of 'plumped' chicken LIVINGSTON, Calif., May 11 /PRNewswire/ -- Amidst an increasing national focus on sodium as a critical health issue, West Coast poultry producer Foster Farms today launches a comprehensive consumer campaign to raise awareness of saltwater-injected or "plumped" chicken.
- The horn of a unicorn considered as a medical or pharmacological ingredient.
- A winged horse with a single horn on its head; a winged unicorn.

subset: commoncrawl


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Welcome to the ongoing geeky adventures of the Craft Addict! Yes, more Dragoncon sewing but I think you'll like this one. My lovely friend Meredith made a passing comment on the Book of Faces one day about sewing a Star Wars pin-up dress with some sheets she'd bought. The shop had recently gotten in some awesome Star Wars fabric so of course I had to use that comment as inspiration for my own geeky dress. If you're going to be… Read more »
Posts Tagged: dress
ModKid Sew-a-thon
There's nothing like having your child go to her grandparents' house to inspire you to sew. And, boy, did I take advantage of the free time. I'm still having a blast cranking out pieces from Patty's new book, Sewing ModKid Style: A bouquet of Bloom dresses, some with coordinating leggings Stretchy and fab Yoga Pants and Shorts A few years ago I went on a knit-buying binge — and then never did anything with the fabric. Man, is it fun… Read more »
I fell in love with Rosie's fabrics for Lecien when I saw them in Houston last October. I've never made my love for all things Melly & Rosie a secret, so it's likely not a surprise that I could not wait to get my grubby, needs-a-manicure hands on some of this yummy fabric. Which is why it's taken me three months to finally buy some. OK, not really. The real reason is that 1) I've been incredibly wrapped up in… Read more »
Summer Whites
It's taken me a while to get around to sewing the Portabello Pixie Analise but I finally got to it this week and whipped up a sweet summery dress for Miss L. I picked up the fabric from Joann's after fondling it over the course of several trips to thte store. (Don't act like you don't do the same thing.) I bought it even though I wasn't quite sure what I'd do with it, although I had an inkling that… Read more »
Costume Quickie
Just a quick peek at the Halloween costume I made Miss L. It's finally finished. Woohooo!
More Fall Dresses
I've been having lots of fun with Katie's Round-Neck Top pattern, which includes directions for making it into a dress. It just sews up so quickly and wears easily for my little preschooler. While my previous corduroy incarnation included pockets, the dress on the left does not yet have them and I'm still waffling. Oh, but I do love it in that soft baby cord! I used two prints I found at Joann's, a black-with-white polka dots and a cute… Read more »
Bell Bottoms
Find more at Wordless Wednesday!
Sew Sweet
Thanks for all the well wishes and kind thoughts sent my way. I'm happy to say that I'm feeling much better and looking forward to getting back into the swing of things today and through the rest of the weekend. So let's get back to a little crafty talk, shall we? We're off to see the wizard! A friend asked if I could pull this together for her daughter. How could I say no? She ordered the Wizard of Oz… Read more »


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An Airbus A380, owned by Malaysia Airlines, lands behind a Boeing 787, owned by Qatar Airways, ahead of the Farnborough Airshow 2012 in southern England in this July 8, 2012 file photograph. (REUTERS/Luke MacGregor/Files)
KHARTOUM: A Kenyan Airways plane with 56 passengers on board made an emergency landing in the Sudanese capital, Khartoum, after an engine caught fire, witnesses said on Wednesday.
Nobody was hurt, but the incident on Tuesday night left passengers stranded in a country difficult for travel because credit cards do not work in Sudan due to U.S. trade sanctions. Banks change dollars only at a very unfavourable exchange rate compared with the dominant black market.
The Cairo-bound Boeing 737-700 took off in Khartoum after a regular stopover following a flight from Nairobi. But it had to return to the Sudanese capital after 20 minutes, three passengers on board flight KQ 320 told Reuters.
"An engine caught fire and the plane suddenly lost much altitude. The pilot made a sharp turn and returned to Khartoum," said Souhair Mohamed Hawala, an Egyptian passenger. "There was panic on board. People were crying or praying."
Other passengers showed what they said were pictures from the damaged wing and engine of the plane.
A Kenyan Airways official said the plane had returned with an unspecified "engine problem" and needed to be repaired in Sudan. "We don't know the cause yet," he said, adding passengers would be booked on the airline's next flight out of Sudan after 24 hours.
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As the offseason wears on, us and Matt Josephs of Blue Line Station (Twitter: 11Matt_Josephs8) will be running a tournament for the Best Ranger since the 2005 lockout. Yesterday we saw #3 Rick Nash beat #14 Steve Eminger. Today's matchup is #6 Petr Prucha vs. #11 Paul Mara.
Petr Prucha (acquired – 2002 draft, 8th round)
Prucha burst on to the scene as a rookie in the 2005-2006 season, becoming the first Ranger rookie to score 30 goals in a season since Tony Amonte 14 seasons prior. The tiny winger (listed at 6′ and 175 lbs, but I think that's generous) won over Ranger fans very quickly with his goal scoring and his blue collar play. Prucha clicked with Jaromir Jagr on the top powerplay unit, and never looked back. He scored 22 goals in his second season with the Rangers, but dropped off and found himself as a healthy scratch for the majority of his final two seasons in New York. Prucha was a healthy scratch so often that he wasn't even included in the Tom Renney line generator.
Prucha was eventually sent to Phoenix with Nigel Dawes and Dmitri Kalinin for Derek Morris. He spent two more years there before bolting for the KHL. During his Rangers career, Prucha had a line of 63-50-113 in 237 games.
Paul Mara (acquired – 2007 trade with Boston)
The Rangers acquired Mara for Aaron Ward in the 2006-2007 season. They mostly wanted someone who would be a better fit for the Tom Renney system than Ward, who just wasn't the appropriate fit here. In Mara, the Rangers got a steady bottom pairing defenseman with some grit. Mara played two more seasons in New York before heading to Montreal, putting up a line of 8-35-43 in 156 games, with 164 PIMs and one glorious beard.
Mara was eventually let go after the 2008-2009 season, when he signed with Montreal. The Rangers decided to let their kids play (Del Zotto, Gilroy, Staal, Girardi) and had enough veterans (Redden, Rozsival) to compliment them.
So who do you think is the better Ranger, Prucha or Mara? Vote thumbs up for the higher seed (Prucha), or thumbs down for the lower seed (Mara). Voting ends at midnight tonight.


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Tea party not the villain in this pieceStory | Aug 10, 2011 | 12:15 PMLike Garrison Keillor, whose column used to appear in The Sun, Dan Rodricks underestimates the power of the tea party in American politics ("Question for tea party: What now?" Aug. 4). He should have read John Malagrin's letter right across the fold ("Tea...
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Reproductive Rights
Reproductive Rights Posts Archive
SUBMITTED BY: Tory Roberts, Friday 06/28/2013, 4:09pm
Texas Republican state senator Dan Patrick is not impressed by Wendy Davis. Despite Davis' all day filibuster of an anti-choice bill, Patrick thinks he is the one that deserves the praise. Patrick, the sponsor of the bill, told Mike Huckabee today that he urged his fellow Republicans to break Senate tradition and stop the filibuster. He compared his action to Jesus' criticism of the Pharisees for placing too much importance on "laws and rules." Patrick went on to encourage other senators to similarly cast off the law, asking, "Are we going to become the modern... MORE
SUBMITTED BY: Miranda Blue, Monday 04/29/2013, 11:25am
Right-wing talk show host Kevin McCullough has a column in the American Family Association-affiliated One News Now today, cleverly titled "Barack Hussein Gosnell." McCollough's argument is that President Obama, by speaking at Planned Parenthood recently, is morally equivalent to accused murderer Kermit Gosnell. McCullough claims that "every woman I've ever spoken to on the matter" agrees with him that Planned Parenthood is not "helpful to women." Instead, he writes, "They say they give women choices -- which they interpret to mean,... MORE
SUBMITTED BY: Kyle Mantyla, Monday 03/25/2013, 11:46am
Last week, Mat Staver and Matt Barber hailed legislation passed in North Dakota and elsewhere aimed at dramatically curtailing the availability and legality of abortion, with Barber proclaiming that Roe v Wade was no different than the infamous Dred Scott decision, calling them "twin bookends of evil" and "shameful decisions that are a blight on America's history": MORE
SUBMITTED BY: Miranda Blue, Friday 12/14/2012, 2:59pm
Former Susan G. Komen executive Karen Handel, the Georgia Republican politician who has been largely credited with the cancer charity's disastrous decision last year to withdraw its grants to Planned Parenthood, spoke yesterday to the Family Research Council. Promoting her book about the episode, "Planned Bullyhood," Handel accused Planned Parenthood of launching an "unprecedented, premeditated, Mafia-style assault" on Komen. Further, she alleges that Planned Parenthood "literally co-opted the color pink" from Komen in a sinister "bait and... MORE
Obama Administration is a Phyllis Schlafly Nightmare: 'Whatever the Feminists Want, the Feminists Get'
SUBMITTED BY: Brian Tashman, Wednesday 09/05/2012, 5:00pm
Eagle Forum founder Phyllis Schlafly yesterday spoke to the American Family Association's Sandy Rios to denounce the Democratic National Platform's support for reproductive freedom and marriage equality. According to Schlafly, "the feminists completely control the Obama administration" and "whatever the feminists want, the feminists get," including endorsements of abortion rights and same-sex marriage. There is "support of everything the feminists want," Schlafly lamented, "It's a very destructive force in our society." Rios... MORE
SUBMITTED BY: Brian Tashman, Tuesday 09/04/2012, 11:45am
While appearing on televangelist Kenneth Copeland's Believer's Voice of Victory, disgraced pseudo-historian once again used his platform on Copeland's show to deliver an attack on gay rights and women's rights, this time saying that marriage equality and reproductive rights lead to a nation's destruction. "If a nation says, 'hey, we want abortion, we want homosexual marriage,' it is going down," Barton said, "anytime you move away from what God says, you are going down as a nation." Watch: Copeland: I don't care who... MORE
SUBMITTED BY: Brian Tashman, Monday 08/13/2012, 12:00pm
In the 2004 presidential election, President Bush used "dog whistle" politics during a debate with John Kerry by subtly linking the Dred Scott case to Roe v. Wade. This year, Republican vice presidential pick Paul Ryan is more explicit about his views. He supported a bill to outlaw all abortions and some contraceptives, and in 2010 he said that the "the Supreme Court made virtually the identical mistake" in Roe v. Wade that it made in the 1857 Dred Scott case: Yet, identifying who "qualifies" as a human being has historically proved to be more difficult... MORE
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Sault Ste. Marie residents and visitors love to take part in and put on festivals. Check out this section for the latest festival information.
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Tommy Lee Ladewig is the Manager of Ladja Properties Gp, LLC and holds active roles in two companies and inactive roles in two additional companies .
Ladja Properties Gp, LLC filed as a Domestic Limited Liability Company (LLC) in the State of Texas filed on Tuesday, June 22, 2004. This corporation is approximately eleven years old according to documents filed with Texas Secretary of State. Ladja Properties Gp, LLC also lists Ladja Properties, Ltd. as General Partner.
Tommy Lee Ladewig's additional active roles include:
Tommy Lee Ladewig is connected to other officers through these corporate roles. One of the top connections is Ann C. Jacobs who is the Manager for Ladja Services Gp, LLC and has interests in other companies, including Manager of Ladja Properties Gp, LLC .
Ladja Services Gp, LLC also lists Ladja Services, Ltd. as General Partner, in addition to Tommy's role of Manager. This corporation was registered 0 years after Ladja Properties Gp, LLC as a Domestic Limited Liability Company (LLC) in the State of Texas on Tuesday, June 22, 2004.
Key roles for Tommy Lee Ladewig
Ladja Properties Gp, LLC
Manager, Director, Member
Ladja Services Gp, LLC
Manager, Director , Member
Aj Cattle Company, LLC
Manager, Member Mmbr
Ladja Services, Ltd.
Ladja Properties, Ltd.


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I have an obsession. If you look in my closet, you would see an obscene amount of backpacks.
Travel backpacks, fabric backpacks, leather backpacks, convertible styles, solids, and prints. I know it may seem over the top, but if you give it a solid try, it's extremely liberating. I have been wearing them for years, but oddly enough, I never used them in school. I went from the "book bag" of the late 70's and early 80's to "pocketbooks" (as we called them in my house) for the rest of my schooling years. Needless to say, I had very toned arms from carrying those enormous textbooks in my hands!... Continue Reading
On this Valentine's Day, I wanted to reshare my story of love!
I am one lucky girl. Not only was I able to find my Prince Charming, but I have a Fairytale Story to go along with it, filled with beautiful gowns and scary dragons! (Ok, well, maybe the pink dragon wasn't THAT scary- but still!)
Langdon and I met while working at Caesars Palace in Las Vegas. I was a magician's assistant, and he seated people for the shows - while wearing a toga, I might add. We had always seen each other around the theater, but were officially introduce... Continue Reading
As the holidays grow closer, so many of us are reaching for our KitchenAid mixers and putting recipes new and old to test for cookie season! This year the host team is doing something extra fun. Each host will share a cookie recipe and tag another host via his/her social page. This "tag" is social savy way of saying, "hey it's your turn to bake!"
So thanks to my friend Melissa Epps, it is time to dish up a new recipe that I am trying out this year. They are Oatmeal, Cranberry, and Chocolate Chunk Cookies! I saw this recipe on foodnetwork.com and I must say they are delicious!
I... Continue Reading
A couple of years ago, I was waiting in line for coffee, and a woman told me she thought I was stylish.
Me? Stylish? She didn't say that she liked my clothes. Rather, she made a statement about my abilities to pull it all together. I remember how I felt when she told me that. I felt fantastic and sophisticated. I wish that I could take full credit for it, but I can't. It all belongs to Lori Goldstein.
(Here I am pumpkin picking wearing our LOGO Today's Special Value)
Lori is the designer of the LOGO collections and LOGO Littles lines of clothing at QVC. Her unique ability to c... Continue Reading
I just had my 12th wedding anniversary, and I can't believe how much my life (and hair!) has changed since that day. Here are a few memories I thought I would share, from a wedding in Las Vegas, and a honeymoon in Tahiti, complete with a perfect rainbow!
The Big Day!
On the grand staircase
My prince
Our honeymoon in Tahiti
A beautiful rainbow on our honeymoon
- Sharon
Find me on Facebook here: www.facebook.com/SharonFaetsch Continue Reading
Fozzie first became a part of our lives a little over 10 years ago. I was a new show host, and my husband had just relocated here from Las Vegas. We had always talked about getting a dog, and this seemed like the time to do it.
There was no debate about the breed. We had both grown up with poodles, and loved the fact that there was no shedding. So, we checked the paper and found a breeder of red poodles about a half hour away. I grew up with an apricot poodle, and had never heard of the red color before, so I was curious. We drove there expect... Continue Reading
A couple of days ago, it was my daughter's 7th birthday. I can't believe how much my life has changed in the last seven years. From sleepless nights and colic, to first steps and first days of school. Now she is reading books about Laura Ingalls Wilder and Martin Luther King Jr.
I can't imagine what is going to happen next! She wanted to have a party at Build-A-Bear this year, and I was a little apprehensive. We had never had a birthday party for the kids outside of our own house before, and I had no idea what to expect. Luckily, everyth... Continue Reading
My Friday Night Meal this week revolves around fresh flavors. When it comes to fish, I usually want a clean flavor with a delicate but slightly firm texture. One of my favorite types of fish is Australis Barramundi Sea Bass. In my opinion, it is more delicious than most restaurant fish dishes, and there are many ways to prepare it.
My favorite is the baking method, because it's so quick and easy. The hard part is coming up with a good side dish to go with it. My family loves broccoli, but I'm always looking for ways to serve it that don't involve a ... Continue Reading
I don't know about you, but I am almost ready for this winter to be over. When it's really cold, and summer vacation is still months away, I sometimes want to take a vacation through the foods I prepare for my family. My Friday Night Meal this week revolves around a dish called an empanada. Whether you had one last week, or have never even heard of one, I can promise you they are delicious! I first sampled the Authentic Argentinian Empanadas a few months ago, and almost lost my mind. The flavors are rich, and because of the way they are crafted, they are very easy... Continue Reading
When the wind is blowing, and it's chilly outside, I love to settle down with a bowl of something hearty. This is the time of year when I look forward to simple and delicious comfort food. I have a quick and easy comfort food meal the whole family will love, and it's from Laurie's Kitchen! You may already be familiar with their delicious soup mixes, but this recipe is all about chili. It's White Bean and Chicken Chili, and you can make it in about 30 minutes. All you really need is 2 cups of cooked, diced chicken, 4 cups of water, and the soup mix. However, I got ... Continue Reading
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text: Wayne Jones v Gary Anderson Visit Bet365 Save For Later by Live Darts StaffPosted Share: 0 0 0 Get Live Streaming Darts Alerts Watch Wayne Jones v Gary Anderson live for free bet365's live streaming darts service allows you to watch live darts games for free. In order to use the live darts streaming service you need to be a member of Bet365. Once you join you simply need to select Sports > Live Streaming and you'll be given access to all major darts tournaments. How to watch the match on bet365's live darts player To watch the free feed of Wayne Jones v Gary Anderson on 20 November 2010 simply: Go to bet365 Log-in Open the bet365 Live Streaming Player Sit back and watch live darts Visit Bet365 Read our Bet365 review Watch & bet on live darts streaming online at bet365 From Around the Site Dubai Darts Masters 2015 TV coverage and live stream - The essential guide of how to watch from May 28-29 PDC ProTour Order of Merit update - Webster joins provisional World Matchplay field Auckland Darts Masters introduced as World Series schedule confirmed facebook Twitter gPlus Send email Reddit More Reddit Su Link All Live Streaming Darts News Recent Live Streaming Darts Articles Michael van Gerwen profile for the PDC World Darts ChampionshipIt is a sign of the strength of Michael Van Gerwen's current form that a player that has never even reached the quarter finals is considered the second favourite.... Group Seven Glory For CavenJamie Caven beat Paul Nicholson to claim the seventh place in the Winners Group of the Championship League Darts... Championship League Darts live order of play – Wednesday 31 OctoberThere's one final Championship League Darts place remaining in the Winners Group, with Michael van Gerwen and Paul Nicholson among those playing today ... Championship League Darts live order of play – Tuesday 30 OctoberMichael van Gerwen and Paul Nicholson are among the players competing for a place in the Winners Group of the Championship League Darts... Championship League Darts live order of play – Thursday 18 OctoberThe Championship League continues on Thursday, with Terry Jenkins and Dave Chisnall among those players battling for a place in the Winners Group... Live Streaming PDC Darts TV DetailsThe 2012 PDC darts season reaches its business end and there are five unmissable live events to look forward to. ... Michael van Gerwen hits a 9 Dart Finish against Steve Beaton (video)Mighty Mike was on fire in the World Matchplay Darts, with the Dutchman hitting a 9-dart finish en route to beating Beaton... World Matchplay Darts live order of play – Thursday 26 JulyPhil Taylor is back on the oche tonight when he plays Ian White for a quarter final spot in the World Matchplay Darts.... 2012 Word Darts Championship live order of play – Sunday 1 JanuaryThe 2012 Word Darts Championship has reached the semi-final stage, with Andy Hamilton playing Simon Whitlock, while Adrian Lewis plays James Wade... More Stories Recent Articles Van Gerwen scoops Dubai Masters hat-trick to continue desert dominanceMichael van Gerwen clinched a third consecutive Dubai Masters title with a terrific 11-8 victory over Phil Taylor in the final of the PDC World Series event on Friday.... Dubai Darts Masters 2015 live scores and tournament schedule of playBig-time darts returns to the desert this week as the 2015 World Series commences in Dubai, and you can stay in touch with all the latest results and fixtures here.... Dubai Darts Masters Day Two preview and order of play - Top four battle for gloryThe world's top four players face off for the right to be crowned 2015 Dubai Masters Champion as the World Series event draws to a close at the Dubai Tennis Stadium on Friday evening.... Dubai Darts Masters 2015 TV coverage and live stream - The essential guide of how to watch from May 28-29Big-time darts returns to the desert this week as the 2015 World Series commences in Dubai, and you can tune in to every match live.... Taylor ends Dubai drought as Van Gerwen, Lewis and Anderson turn on the styleMichael van Gerwen began his bid for a third consecutive Dubai Masters title with a comeback win over Raymond van Barneveld on Thursday, as Gary Anderson, Phil Taylor and Adrian Lewis also recorded superb victories.... Confident Van Gerwen sets his sights on Dubai Masters hat-trickMichael van Gerwen says he is relishing a return to action at the Dubai Masters this week as he hunts down a third consecutive title in the Middle East.... Dubai Darts Masters Day One preview and order of play - Elite eight begin World Series campaignsThe 2015 Dubai Masters commences on a red hot Thursday evening, with four unmissable Quarter-Final ties kicking off proceedings in the Middle East.... PDC World Cup of Darts 2015 teams and draw - Netherlands to launch title defence against USADutch duo Michael van Gerwen and Raymond van Barneveld will begin the defence of their PDC World Cup title against the USA in June's 32-nation event in Frankfurt.... Anderson hoping to handle the heat on his Dubai Masters debutGary Anderson says his toughest battle at this week's Dubai Darts Masters will be against the searing desert heat as he prepares for his World Series debut.... More Stories Tags Gary Anderson, Wayne Jones


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485 Fifth Ave.,
New York, NY 10017
King, $229-$265; XL View King, $329-$365; Large Loft, $429-$465; Splash Suite, $579-$615; Large Two-Bedroom Suite, $1429-$1465; XL Terrace, starting at $3,229-$3,295
B, D, F, M at 42nd St.-Bryant Park; 7 at Fifth Ave.
$60 for overnight; no in and out
American Express, Discover, MasterCard, Visa
Hyatt's boutique Andaz brand launched this midtown outpost in July 2010, not long after the opening of its Wall Street location. In lieu of a front desk, guests here check in over a drink in the lobby lounge with one of the roving, touch-screen-computer-equipped staff members, who double as concierges. Rooms are located in both the main towerstill bearing the limestone-and-brick façade of the 1916 Rogers Peet Buildingas well as a second connected building, the elevators for which are accessed on the second floor, past a series of airy, glass-walled event and lounge spaces. Designed by Tony Chi, the large, bright guest rooms (some of which feature views of the New York Public Library, and some of which come with landscaped terraces) evoke city lofts with twelve-foot ceilings (even in the bathrooms), unadorned beds and walls, and pale wood tables. Bathrooms, meanwhile, boast plus-size C.O. Bigelow products and a porcelain foot bathalong with a bench and separate knee-high faucetin the glass-enclosed showers. Off the lobby, The Shop bar and restaurant's menu lists the purveyorsfrom local delis to regional farmsof many of the ingredients used in its upscale comfort food.Pros
Complimentary snacks and nonalcoholic drinks from the minibar; large, quiet rooms; free WiFi; central location; foot baths in the showers; 24-hour gym.
No spa; the high-tech TV, Internet, and entertainment system can be tricky, so use it carefully or you'll be charged.
There's never been more lodging here, and the rooms have never looked this good.


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ApplianceFrigidaire Refrigerator
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Age of Appliance5 - 10 years
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Vernier Watches
Women's Oversized Rose Gold Watch
- Color:
- rose gold
Please select an available size.
Please select a size.
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- Round rose gold tone base metal case with rose gold tone dial and chronograph subdials at 3, 6, and 9 o'clock
- Rose gold tone base metal bracelet with jewelry clasp closure
- Japanese quartz movement
- Water resistance tested at 10M
- Case measures 40mm (1.57 inches) wide
- Bracelet measures 22mm (0.86 inches) wide
- 5 year limited manufacturer warranty
Material: Rose gold tone base metal
Brand: Vernier Watches
Origin: Imported


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February 13, 2009
Loving the burrito…
by Ethan Oberman with 0 comments
I have been eating a lot of burritos lately and decided that it is fastly
becoming my favorite food. There is nothing more tangible – wrapping your
hands around the warm tortilla shell packed tightly with rice and beans
(always 'mashed' or refried as they are called in the States) and meat and
beautiful green guacamole and salsa and sour cream and cheese softly melting
There is a special place I go – measuring about 300 square feet. The
majority is devoted to cooking space except a small sliver where patrons come
in, order, and wait. The fire is always hot beneath the grill, there are
always big pots boiling over on the stove, and always hardworking hands
preparing food and tending to various tasks. In all the times I have gone – at
all hours of the day – I remain amazed by the perfectly pressed shirt, neatly
slicked back graying hair, quietly trimmed mustache, and gently balancing
glasses of the gentleman who sits patiently behind the register.
With a warm grin, he oversees the operation – speaking passionately and
lovingly about the food they prepare. In my visits I can recall vividly our
conversations about all matter of things. We spoke once for a half hour about
tortillas, how his are prepared, handmade, and why they are able to better
withstand the heat of the fuel packed tightly within. We spoke about the
origin of the burrito, how it came to be a common food of northern Mexico, and
how it moved northward with the migration of workers during harvest season. We
spoke about 'mashed' beans, how they are prepared, how they are spiced, and
how the consistency is maintained without adding additional lard. We spoke
about family, where he was from, how he ended up in this little storefront on
the western coast of California. From our numerous conversations, it is
apparent that he has a great deal of respect for his life, what he does, and
his journey.
In thinking about all this, I am reminded of something that I hear more
often these days. I hear it mostly echoing over our IRC channel when I ask a
question about this or that – something I often don't know or understand. And
it is not limited to one particular name or instance but all have remarked and
suggested this course of action over time. And it rings: 'look it up on
wikipedia'. Of course I – of all people – understand the power and wonder and
majesty of the Internet. And of course the information garnered from the
gentleman above lives within the hundreds of thousands of indexed and
cross-referenced wikipedia pages. In the end, however, I always prefer the
tangibility of open discourse – it makes everything taste better.


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- 9 months ago Winning. Awesome phone for the best mobile os!!!
- 11 months ago Well, nice sellection of apps, used two of them (Sleep bug and Relax melodies) they were good but right now use this app...
- 11 months ago Great selection, however this game Animal Game For Kids should be on the the list. Nice work WPcentral!!!
- 11 months ago Finally good news from Microsoft!!!It was bloody time to get the propper treatment for the rest of the world!!! http://www...
- 12 months ago My laptop just died, so this will be very good replacement
- 12 months ago Winning for sure :D
- 1 year ago Well you can put this app to the list "Baby Phone" since is simply awesome. Lots of fun for my little daughter!!!www....
- 2 years ago Got the second update and everything seems to work just fine.Nice work Microsoft
- 2 years ago Used the tool and got the update after the 3rd attempt. Everything is ok, however the lock+wallpaper option is not working...
- 2 years ago Awesome!!!
- 2 years ago I will go for Nokia Lumia 920
- Member for
- 2 years 6 months


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1995 Dodge "Neon" Factory hand built race car for the Dodge Challenge series; ACR with VIN #01; raced in "Celebrity" Challenge races; purchased by... More
West Coast Classics are proud to present an extremely rare original southern California owner example of this 1984 Mercedes 280SL roadster with very desirable... More
1955 Jaguar XK-140 Open Two Seater (OTS) with less than 5 hours since a five-year professional rebuild by Vintage Jag Works. This is probably... More
1952 Jaguar XK-120SE, Full frame-off restoration (1995) by Pebble Beach Concours winner; minimal mileage since restoration. New cinammon interior leather (2013) and muffler (2014). Engine... More
1986 Alfa Romeo Spider Veloce Champagne Metallic with Black and Tan Leather Interior 55,000 Original Miles, Never any Rust or Collision New show quality paint, premium cloth... More
This is an X-MOD Left Hand Drive 110 that was part of the British Army's Tithonus Project.A select group of 110s were chosen to... More
There were only 11 Eleanor Mustangs built for the 2000 hit film Gone in 60 Seconds, only 3 of those were drivable, and after... More
A collector's item from the moment they were announced, the 2007 Shelby GT500 has always been a desirable coupe. Of course it only... More
Running car parked in 1988. Poorly Stored outdoors under tarp for the last 5 years. 4 cylinder flathead with 3 speed and overdirve. Missing ... More
This classic 1964 Corvette coupe is a California car which has had a partial older body-on restoration. Exceptional single-stage urethane paintwork in correct Riverside... More
1968 Porsche 912 LWB '68 Porsche 912 Long Wheels Base Coupe with matching numbers chassis#12802682 engine#1282510 in red with black interior. Equipped with a... More
1976 Rolls Royce Silver Shadow LHD '76 Rolls Royce Silver Shadow LHD in old English white over burgundy with burgundy interior with white piping. Equipped... More
1969 Jaguar XKE Roadster '69 Jaguar XKE Roadster with matching numbers in Primrose yellow with biscuit interior and canvas soft top. Equipped with a manual... More
1958 Austin Healey Bug Eye Sprite '58 Austin Healey Bug Eye Sprite shown here in blue with red interior. Comes with the soft top... More
1961 Mercedes Benz 190SL '61 Mercedes Benz 190SL Roadster in white with red interior. Very attractive color combination complimented by a black canvas soft... More


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LORING PARK — You can't see it from street level, but there's something historic happening on the roof of the Minneapolis Convention Center.
Over the past few months, crews have been busy installing and activating 2,613 solar panels atop the building. When the project wraps up at the end of November, it will be the largest solar photovoltaic system in the Upper Midwest, estimated to generate 750,000 killowatt hours of renewable energy per year. That's enough to power 85 houses annually — and enough to offset carbon dioxide emissions given off by 60,587 gallons of gasoline.
The Convention Center's solar array is another green jewel in the crown of a city eager to tout its progressive environmentalism. Minneapolis already has three other facilities powered by solar arrays: the Currie Equipment Facility in North Minneapolis; Fire Station No. 6, a mere block from the Convention Center; and the Royalston Public Works facility.
In praising the Convention Center's array, Mayor R.T. Rybak also cited other Downtown sustainability efforts, like Target Field's green roof.
"The Convention Center is Minneapolis' living room, where we welcome thousands of guests a year," he said. "Now the first message these guests will get is that Minneapolis is a leader in the clean-energy economy."
Meet Minneapolis spokesperson Kristen Montag said that each panel began producing energy immediately for the Convention Center.
"They're being hooked up as they go," she said.
Montag added that, once the project is finished, an instructional kiosk will be installed in the building's lobby, offering readings of how much solar energy is being produced and explaining how the array works.
— Gregory J. Scott
Cash-free parking meters to start appearing Downtown
NORTH LOOP — In a move sure to bring sweet relief to Downtown visitors, the city has begun a three-year project to replace old-fashioned, quarter-gobbling parking meters with new electronic ones that accept credit cards.
Crews installed the first "smart meter" on the block of 2nd Street North and 2nd Avenue North on Nov. 9. By the end of November, 46 pay stations, accommodating a total of 450 parking spots, will be up and running throughout the North Loop. Older meters will stay in service until then.
Each meter serves as a pay station for multiple parking spots, with only one machine per city block. Drivers simply punch in their space number and then pay with either a credit card or cash. The new devices are completely solar-powered and are linked up via the city's Wi-Fi network.
Next year, plans call for the installation of another 200 pay stations, spanning a total of 2,000 parking stations. Another 200 stations will come in 2012.
— Gregory J. Scott
City opens Emergency Operations Training Facility
FRIDLEY — When the Interstate 35W Bridge collapsed in 2007, one of the lessons the city learned was that its Emergency Operations Center, located in the basement of City Hall, was too small to serve as a center for large-scale emergencies.
Flash forward three years, and the city has its replacement: a building with 2,800 more square feet of floor space built on a 12-acre lot in Fridley. The new Emergency Operations Training Facility opened early November.
The facility houses training classrooms for Minneapolis firefighters and metro emergency managers, a strategic information center for the Minneapolis police, a training site for the State of Minnesota Structural Collapse Team and a back-up Emergency Operations Center for the state, Hennepin County and City of St. Paul.
The U.S. Coast Guard will also use the facility as a monitoring location for cameras placed along a segment of the Mississippi River from St. Louis to the Twin Cities.
— Gregory J. Scott
A bonus for your bus ride
Metro Transit launched Ride to Rewards in November, a new rider loyalty program the bus, commuter train and light rail operator says is the first of its kind in the country.
The program is free for all Go-To Card holders age 18 or older, who will earn points redeemable for future fares or gift cards when they sign up for the program. Go-To Cards are rechargeable plastic fare cards that come in a variety of flavors, including the U-Pass and College Pass for area college students and Metropass offered by some Twin Cities businesses to their employees.
One week in, Ride to Rewards already had about 2,000 participating transit users, said Bob Gibbons, director of customer services for Metro Transit.
"That's pretty good because all we did so far was Tweet it and issue a news release," Gibbons said, adding that Metro Transit planned to use e-mail alerts and other means to attract new participants "in increments," so as not to overwhelm ridetorewards.com, the program's new website.
Once enrolled in the program, Ride to Reward participants earn a half-point every time they swipe their cards to pay for a ride on a bus or train, with the exception of transfers. Workday commuters who board once in the morning and once in the evening will earn one point every day.
The conversion of points into Go-To Card credit or gift cards is explained on the program's website. If you do the math, each ride, or half-point, works out to roughly one cent of Ride to Rewards value.
That means the hypothetical workday commuter earning one point a day, five days a week, would require about 23 months to bank the
500 points required to earn $11 in Go-To Card credit. But the program isn't designed to operate on rides alone, Gibbons said.
Points accumulate much faster when Go-To Card holders make purchases at Ride to Rewards' merchant partners with a registered credit or debit card. Every dollar spent with that card at a participating business earns one point or one half-point.
The list of business partners was still fairly short a week after the Ride to Rewards launch — only 13 businesses at 23 individual locations as of Nov. 8 — but was expected to grow, Gibbons said.
Transit Treasures, a Florida-based start-up company, runs Ride to Rewards at no cost to Metro Transit and recruits new merchants into the program.
The "Daily Leaderboard" at ridetorewards.com gives program participants an easy way to gauge their point-collecting abilities against the top earners.
A week after the program launch, several of Ride to Rewards' top rider-spenders already were nearing 300 points.
— Dylan Thomas
Mill District City Apartments now open
MILL DISTRICT — A posh rental development that area real estate agents, city officials and business leaders have been fawning over for months is finally open to tenants.
Michigan-based developer Village Green hosted a ribbon-cutting ceremony on Nov. 10 for its new Mill District City Apartments. The $25 million, 175-unit project is expected to draw 250 new residents to a neighborhood starved for rentals. It also closes up one of the final holes in the burgeoning Mill District, filling a parcel of land at 225 Portland Avenue that languished for years after a previous project stalled during the real estate implosion of 2006–07.
The development is Village Green's third rental project Downtown, following the Eitel Building City Apartments and the Loring Park City Apartments. Accommodating a long unmet demand for Downtown rentals has been Village Green's theme in the run up to this month's opening. The rental occupancy rate for Downtown has hovered above 95 percent nearly all year. But the Mill District apartments represent the first new construction rental development in years.
"The companies here are dynamic, and they're hiring again," said Village Green CEO Jonathan Holtzman. "The renter wants to be Downtown, wants to work for these companies. Everything is here."
Holtzman has argued that the building's boutique amenities — initial plans call for a green courtyard with an indoor/outdoor swimming pool and an al fresco movie theater, an underground heated garage and extended-hour concierge service — will act as a recruiting tool for companies trying to lure young talent here.
In turn, the thinking goes, these young professionals will spend their salaries at area bars and restaurants, easing into a Downtown environment where they might eventually buy a home. And the project is estimated to generate $350,000 annually in real estate taxes. Such a perceived payoff has resulted in big cooperation from the city.
When Holtzman struggled to secure financing, City Council Member Lisa Goodman (7th Ward) helped broker a deal to defer land sale proceeds. The city is deferring about $1.4 million for up to 24 months, and in exchange will receive a promissory note from Village Green.
U.S. Bank ultimately provided a loan, and the entire project is nearing completion just over a year after an October 2009 groundbreaking.
"We are open for business," proclaimed Elliot Jaffe, bank president of the Twin Cities market, touting the development as a ray of light in a still-tough credit market.
The first tenants moved in Oct. 25, according to a Village Green spokesperson. The building is currently 33 percent full. The company's website lists 500-square-foot studios starting at $1,045 per month. Three-bedroom units go for as high as $2,465.
— Gregory J. Scott
City promotes radon testing
Just before Halloween the city gave away free radon testing kits in an event at one of its Downtown administrative buildings.
It was appropriate timing, because the facts on radon can seem a little scary.
The Environmental Protection Agency reports radon-related lung cancer kills 21,000 people on average every year. That makes radon the leading cause of lung cancer among non-smokers and second only to cigarettes in causing cancer among smokers.
Radon, a naturally occurring radioactive gas, is common in the mineral-rich soils of the upper Midwest. Colorless, odorless and tasteless, it seeps up through the ground and can collect in Minnesota's well-insulated cold-climate homes, according to the Minnesota Department of Health.
The state Health Department further reports about one-third of Minnesota's homes have elevated levels of radon that, after years of exposure, could pose a significant risk to residents. The good news is that mitigation can significantly reduce the amount of radon entering a home.
The first step is to buy a radon kit to measure the radon levels in your home. They could hardly be easier to use; typically, they just hang in the basement for a few days before they're mailed in to a testing center.
The testing kit giveaway was a one-day event, but the city is still selling the kits for only $9. They're available for purchase 8 a.m.–4 p.m. Mon.–Wed. and Friday in room 300 of the Minneapolis Development Review building, 250 S. 4th St.
Visit the Minnesota Department of Health's radon page (health.state.mn.us/radon) for more information on testing and mitigation.
— Dylan Thomas
City likely to proceed with controversial waste facility
HOLLAND — The city's chosen site for a controversial hazardous waste facility in Northeast is almost a done deal.
City officials recently signed a purchase agreement to buy land for the facility on University Avenue and 27th Avenue Northeast. As this story was filed, the city was in the middle of a 45-day "due diligence" period before closing.
"I don't expect that there will be anything on the property that would prevent the city from finishing that sale," said Susan Young, director of the city's Solid Waste and Recycling division.
The city and county are teaming up to demolish the old Wheeling-Pittsburgh Steel Corporation campus at 340 27th Ave. NE. In its place they would construct two buildings to serve as drop-off points for household construction debris and hazardous waste like paints and flammable liquids.
As the city prepares to close, a grassroots advocacy group is taking steps to fight the facility. Marie Zellar, a volunteer with Don't Dump on Northeast, said businesses like Gasthof's and Jimmy's Bar are joining the cause, and the group might throw a benefit concert night to raise money for an attorney. An attorney would help them argue that the facility would be unlawful because of improper zoning.
The city's zoning administrator maintains that the site does have proper zoning, and the city attorney's office is currently writing a report to that effect.
The group also contests the "recycling center" label.
"We're going to have a bit of a brawl on that," Zellar said.
The city has a new Northeast support base in its corner, however.
An independent group called Rethink Recycling Northeast! formed in the fall. The organization's goal is to raise awareness of what the government is "actually" proposing, according to its Facebook page.
"We're just generally supportive of the service being proposed here in Northeast — properly disposing of household hazardous waste materials so they don't eventually end up in our air, water and soil," the site stated.
Northeast might be ripe for debate, but the city has already resolved one point of contention — officials decided the facility would never take on overflow waste from the Hennepin Energy Recovery Center, as was originally proposed. Kotke said the emergency use would have been a remote possibility — perhaps occurring once in 20 years — but they decided to eliminate that possibility in response to neighborhood concern.
"We'll have to find some other location for that," he said.
Kotke said an extensive public participation process is still to come.
"We haven't actually designed the facility yet," he said.
The facility would be closed on Sundays and Mondays. A recent traffic study determined that the facility could average more than 600 trips per day, but it would take years to reach that level. According to the study, University Avenue should be able to handle the new traffic without extra delays.
— Michelle Bruch
New executive director for Center for Book Arts
MILL DISTRICT — It took six months and a nationwide search just to hire the guy already doing the job.
After serving as interim executive director of the Minnesota Center for Book Arts (MCBA) for six months, Jeff Rathermel has locked in the position permanently. On Oct. 26, the MCBA board officially granted him the promotion.
Rathermel who holds Masters degrees in both fine arts and public affairs and nonprofit management, has served as MCBA's artistic director since 2004. According to the board's official statement, Rathermel's ascendance is a sign that the organization is "refocusing its mission on the artist"; Rathermel has taught art at the College of Visual Arts and MCAD, and his own work has been collected by Walker Art Center and Yale University.
But the promotion is also a sign that MCBA is happy with its current accomplishments — a tad surprising, given that its board has long complained of the Center's low public profile.
Amongst book artists, the 25-year-old MCBA is believed to be the largest and most comprehensive organization of its type in the country. And it's housed inside Open Book, itself a national rarity as the only facility that combines the literary and book arts community. Indie publisher Milkweed Editions and the Loft Literary Center share the building.
But still, MCBA's renown often comes as a surprise to people. Some believe the low profile is what caused the previous executive director, Dorothy Goldie, to be dismissed earlier this year.
"Marketing is always a problem when you're a nonprofit with limited resources," said Rathermel. "We need to think of ways of targeting audiences more with our offerings. Our constituency is very broad."
Rathermel's strategy is to have MCBA jump into the community event game that has been successful at museums like the Weisman and the Walker. In 2009, he launched the Book Art Biennial, an every-other-year bonanza that culminates in the awarding of the MCBA prize. It's the "first international artist book prize in the world," says Rathermel. In 2009, it went to Veronika Schaepers, a German artist working in Japan. The Biennial will return in July 2011.
"We have programs for emerging artists through the Jerome Foundation, which is great," he said. "But we need to look at post-emerging. How can we support the artists that are experts in the field?"
The other feather in Rathermel's cap is the MCBA Collector's Forum, launched just last month. The Forum is a connoisseur's club, designed to develop and nurture a community of public and private collectors. The Forum hosts a Fine Wine and Fine Books event, as well as a series of lectures tailored to new collectors. The talks focus on what's on view in the MCBA gallery and often highlight a local or visiting artist.
Rathermel confirmed that there will not be a new artistic director. He will continue to direct the artistic vision of the organization in addition to his executive director duties.
— Gregory J. Scott


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3D Iron text effect tutorial in Photoshop. Iron text tutorial has been carried out in Adobe Photoshop CS5. The 3D option in older versions may differ. This tutorial is full of useful tips and tricks for Photoshop users. I will use 3D to create iron text effect.
1. Open a texture image in Photoshop. Width 600 and Height 340.
2. Go to: Image > Adjustment > level
3. Go to: 3D > New 3D Postcard from Layer.
4. Go to: Window > 3D
5. Select 3D Materials.
6. A dialogue box will appear; Name the new material as 'Damage'.
7. Now create your text using white color. The font is 'Cooper Black.'
8. Go to: 3D > Repousse > Text Layer.
Make the following changes:
It should look like this.
9. Select both layers.
10. Go to: 3D > 3D Merge Layers.
As the two layers merge, the text will disappear. But that's alright.
11. Go to: Window > 3D
12. Open the 3D (Mesh) tab.
13. Select your text layer (Damage) from the two options (see below).
14. Choose the 3D Mesh Slide Tool.
15. Slowly drag your cursor across the image vertically. Before you do this, ensure that the selection is on Damage and not the texture area. Your text will reappear. Rearrange it to look like the image below. It must not bulge out too much.
16. Select 3D Light.
17. Select Spot lights, click on create new light and choose New Spot Light.
Make the following changes:
18. In the 3D (Scene) tab, scroll up and select Scene and make the following changes:
This will smoothen the edges of the text.
Final Picture:


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ATM OG Mint 7.75" Skateboard Deck
Item # 240361
--- Spend less for this item ---Buy more and save
Stash Points: 2,995
What is this?This is the number of points you get in The Zumiez Stash for purchasing this item. Stash points are redeemable for exclusive rewards only available to Zumiez Stash members. To redeem your points check out the rewards catalog on thestash.zumiez.com.
Ride in dank style with a white pot leaf graphic print on a mint deck with slight concave for solid pop.
|More Details


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Latest Large Hadron Collider Stories
Scientists working at CERN's Large Hadron Collider are gaining a better understanding of the primordial universe through experimentation involving the use of heavy ions.
After years of theories and speculation, the Higgs boson particle discovery was announced on July 4, or was it? Several caveats swirled around the somewhat anti-climactic announcement, and almost a month later many scientists familiar with the CERN project are still reluctant to definitively say what they found was the so-called 'God Particle'.
Success at the world's largest particle accelerator LHC / Experiments involving scientists from Mainz University show first direct evidence of the Higgs boson
Now that the 'God Particle' has been discovered, is it time to pack up CERN's Large Hadron Collider (LHC) and move on to other projects?
About ten years ago British physicist Stephen Hawking was at a conference in South Korea when he made a small wager with University of Michigan physicist Gordon Kane that the long-theorized Higgs boson particle did not exist, a bet that Kane honored four years later, by sending the world renown physicist a check for $100, acknowledging that there was no Higgs.
Scientists at Europe's CERN research center have revealed the discovery of a new subatomic particle, one that could be the elusive Higgs boson or "God particle."
Before Wednesday's big CERN event, Tevatron scientists have admitted that they are still uncertain about the existence of the God particle.
CERN will present preliminary findings from its search for the Higgs Boson, the so-called 'God particle', on July 4 as an international physics conference gets underway, the Geneva-based organization said on Thursday.
- An uxorious, effeminate, or spiritless man.
- A timorous, cowardly fellow.


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To embed, copy and paste the code into your website or blog:
This week, FinCEN published its semiannual SAR Activity Review, which provides information about the preparation, use, and value of Suspicious Activity Reports (SARs) filed by financial institutions. The report identifies SAR trends, reviews law enforcement cases that demonstrate the importance and value of Bank Secrecy Act (BSA) data to the law enforcement community, and highlights issues related to financial exploitation of older Americans. FinCEN also published an annual companion report, "By the Numbers," which compiles numerical data gathered from SARs filed by financial institutions.
DISCLAIMER: Because of the generality of this update, the information provided herein may not be applicable in all situations and should not be acted upon without specific legal advice based on particular situations.
© BuckleySandler LLP
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Eat, Drink and Be Chary
J.M. Coetzee's new novella, The Lives of Animals, must be some kind of first. Usually when a work of fiction comes to us wrapped inside critical essays like a knife inside cardboard, the work has been published many times and the author is long dead. But here is a novella surrounded, in its first edition, with essays written by prominent academics. A literary critic, a primatologist, a historian of religion and a theoretician of animal rights have all been called in to figure out what Coetzee is up to.
If Coetzee, the South African novelist whose best and best-known book may be Waiting for the Barbarians, were an animal, he would be a fox--quick, aloof and crafty. In 1997 Coetzee was invited by the Princeton University Center for Human Values to give a pair of lectures. Instead of doing so, he presented his audience with a novella about a famous novelist who is invited to speak at a prestigious American college. Rather than discuss literature, the fictional novelist--an Australian woman named Elizabeth Costello--lectures her audience on the importance of animal rights, the moral necessity of vegetarianism. Costello's audience is, as Coetzee's was, a bit surprised. But while Costello is all over the place--here eloquent and astute, there slack in her reasoning and a bit hysterical--Coetzee is nowhere to be found. He sets the scene and retreats from it, and when, over an awkward dinner at the Faculty Club--only three people dare to order the fish--Costello defends her vegetarianism in strong terms and things threaten to turn acrimonious, we have little idea of where Coetzee's own sympathies lie.
For all the metafictional high jinks, the self-reflexive character, of Coetzee's story, there is no postmodern playfulness to it. The few jokes are academic in-jokes. The Lives of Animals partakes of Coetzee's usual clipped and somber moral seriousness, and in that sense, much as Coetzee may have surprised his audience, this book is of a piece with his others. Indeed, animal rights and ethical vegetarianism are natural subjects for him. The debate about them turns on questions of suffering, something to which Coetzee's sensorium is pitched with particular keenness. The narrator of Waiting for the Barbarians tells us what any Coetzee narrator might, that his ear is "tuned to the pitch of human pain." Coetzee's prose is able to register physical pain, and the wrack of moral confusion, so acutely that we must sometimes set his slim books down. The chicken-killing scene in Age of Iron is enough to show us that his sympathy is not confined to human pain; and if the chief problem for animals, when it comes to suffering, is that they cannot ask for mercy, the inarticulateness of Coetzee's damaged Michael K (of Life & Times of Michael K) is enough to show us that animals are not always alone in this.
Elizabeth Costello has, like Coetzee, pricked her ears up, she feels, to a sound that no one else hears. How else could the colossal suffering of animals--"what is being done to animals at this moment in production facilities (I hesitate to call them farms any longer), in abattoirs, in trawlers, in laboratories, all over the world"--fail to evoke a universal outrage? It does not seem to her that the capacity to reason by itself confers rights on creatures, and, in any case, do we not teach experimental subjects the most cracked sort of reasoning? The subject ape is not encouraged to wonder, about his captor, "Why is he starving me?" but to think: "How does one use the crates to reach the bananas?"
From the purity of speculation (Why do men behave like this?) [the ape] is relentlessly propelled toward lower, practical, instrumental reason (How does one use this to get that?).... A carefully plotted psychological regimen conducts him away from ethics and metaphysics toward the humbler reaches of practical reason.
This is one of Elizabeth Costello's most interesting ideas, that experiments on our fellow primates should lead not to either party's enlightenment but to their intellectual, and our moral, corruption. The rest of Costello's arguments are less original. She is often vague and distraught, uttering commonplaces. The fascination of Coetzee's novella is not in its arguments but in how those arguments assort with character--something to which I will return.
The Lives of Animals is not the first place to go in search of the vegetarian's rationale. The case for vegetarianism rests, at any rate, on familiar arguments. Humans have been killing and eating other animals for an immemorially long time, and the opponents of this practice have had the same expanse in which to work out their critique. Kerry Walters and Lisa Portmess's very useful and readable Ethical Vegetarianism anthology consists of short excerpts from the writings of notable exponents of vegetarianism (not all of whom managed to forswear steak) and ends up demonstrating the substantial continuity of vegetarian thought. The nineteenth-century New England reformer William Alcott complains of the inefficiency of raising crops to feed slaughter-animals rather than humans directly, much as Frances Moore Lappé will later do, with more statistical sophistication. Peter Singer (the Princeton bioethicist who responds in the Coetzee volume with a short story of his own) offers a glimpse of the veal-calf pen hardly less vivid and painful than Tolstoy's account of a visit to a slaughterhouse. Plutarch finds that cruelty to animals fosters cruelty to humans; Carol Adams's feminist version of this argument is to suggest that we abstract meat from animals in a way analogous to our abstraction of sex from women. Over many centuries vegetarians have argued that meat-eating is cruel and inefficient; that it does nothing for our own physical health; and that slaughter and incidental torture, or the willful ignorance of the same, accustom us to a brutality not always reserved for brutes. What these arguments have going for them is power, not novelty.