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Castle of Illusion Starring Mickey Mouse
By Feral Interactive Ltd
Open the Mac App Store to buy and download apps.
One of the "Best Games of 2014" in the "Mac App Store Best of 2014"!
Leap into a magical Disney adventure!
Before you buy, expand this description to check the system requirements below.
When Minnie Mouse is kidnapped by the jealous witch Mizrabel, it's up to Mickey to rescue her from a hexed castle in this fairytale platforming adventure. Bounce, jump, swim and swing through fifteen captivating levels, each filled with tricky tasks and imaginative enemies.
Overcome the outrageous obstacles Mizrabel has scattered throughout her magical stronghold as you play through a spellbinding adventure where sparkling graphics, charming environments and deft animations evoke the enchanting spirit of classic Disney.
Key features:
• Experience an ingenious re-imagining of the classic 2D platforming of SEGA's original Castle of Illusion Starring Mickey Mouse, with up-to-date graphics, surprising new levels and seamless transitions into full 3D gameplay.
• Bounce across treetops, scale castle walls, dive into flooded dungeons and navigate whirlpools in enormous teacups in your heroic quest to rescue Minnie!
• Launch projectiles and jumping attacks to see off Mizrabel's minions, including floating ghosts, mischievous bookworms and an army of toy soldiers!
• Brave the castle's most powerful guardians – including a grumpy old oak tree, a crazy Jack-in-the-box and a hungry dragon made out of candy – in outrageous boss battles.
• A hidden treasure! Castle of Illusion Starring Mickey Mouse is a legendary game for an astonishing price.
Important information regarding Castle of Illusion:
• Please note that we cannot reply to comments or questions left in Mac App Store reviews. If you require assistance, please contact our support team at email@example.com
• Minimum System Requirements: Processor: 1.8GHz, RAM: 4GB, Graphics: 256MB, Free Space: 900MB.
• The following graphics cards are not supported: ATI X1xxx series, ATI HD2xxx series, Intel HD3000, NVIDIA 1xxx series, NVIDIA 3xxx series, NVIDIA 7xxx series, NVIDIA 8xxx series and NVIDIA 9xxx series.
• This game is not currently supported on volumes formatted as Mac OS Extended (Case Sensitive).
• You can find out more about the game on the Castle of Illusion mini-site at feralinteractive.com/castleofillusion
© SEGA. © 1990, 2013-2014 Disney. SEGA and the SEGA logo are either registered trademarks or trademarks of SEGA Corporation. Developed for and published on the Mac by Feral Interactive Ltd. Mac and the Mac logo are trademarks of Apple Inc. registered in the U.S. and other countries. Feral and the Feral logo are trademarks of Feral Interactive Ltd. All other trademarks and copyrights are the property of their respective owners. All rights reserved.
Customer Reviews
Perfect, but . . .
I don't have any gaming systems, so I don't really know what's on the market in terms of 3D games, but this absolutely wonderful. As a kid I loved 2D sidescrolling games (athough I did not play the original of this game). I never made the transition to 3D. This game was great in that the 2D action (animated in 3D) was amazing as were the 3D action parts.
There were some moments in this game where I had these tinges of childhood—the most magical parts of childhood. It wasn't that I had seen anything in this game in childhood, it was just that the game was magically produced with something you can't force. I see kids shows on TV today and they don't have this magic. This brought me back to what I remember the wonder of cartoons felt like as a child.
As for my title (Perfect, but), my main complaint is that the developers built this amazing beautiful world but that it's now over! I would love to see more levels added, even if they don't create brand new worlds. I love the milkshake world (within the Library world). Make ten more levels of that!
I went back through and got all 800 diamonds to see if that added any features, but it did not. I look forward to more games like this and maybe more levels from this game!
I haven't played a game as Mickey mouse since MICKEY'S MAGIC WAND game on the game boy! The music is cool, the game is challenging and fun! I am only on the second stage but I'm enjoying it! The story is great and (from a scriptwriter's point of view) the villian's name is awesome! The only reason I gave it a 4/5 is because the intro movie could be a little better. It's done with still frames and would have so much more fun to watch as an acctual video/movie! But that's my only complaint! I LOVE THIS GAME! And I got it the day it came out! So worth $15. It's fun for kids, Disney lovers (like me) and adults who love playing video games!
Currently won't run at 5K on 5K iMacs
I tried to start the game twice at 5K resolution, but it crashes each time. I guess the game isn't ready to run at that resolution on the new 5K iMac, but the option is offered in the video settings. Developers please fix this, I would really like to be able to run it on my new 5K iMac.
Edit: BTW, it will run at 4K though and it looks glorious! I fought the first boss and it just looks so beautiful on the 5K screen. If you have a 5K iMac, buy the game anyway and run it at 4K for now.
- $14.99
- Category: Games
- Released: Jul 24, 2014
- Version: 1.0
- Size: 765 MB
- Languages: English, French, German, Italian, Japanese, Spanish
- Seller: Feral Interactive Ltd
- © SEGA. © 1990, 2013-2014 Disney. SEGA and the SEGA logo are either registered trademarks or trademarks of SEGA Corporation. Developed for and published on the Mac by Feral Interactive Ltd.
Compatibility: OS X 10.8.5 or later


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Diplomatic Area 317 P.O. Box 5831 Manama, Bahrain
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Hotel Email: firstname.lastname@example.com
Bahrain International Airport (BAH)
- Distance from hotel: 3.11 MI/ 5 KM East l
- From the airport take the first exit towards the airport street and follow the sign to Sheikh Hamad bridge, cross the bridge turn left to the diplomatic area. The hotel is located to the left hand side.
- Car Parking Available
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- The hotel has newly built multi storied car park. Ample free parking space available for in-house guests and guests using hotel facilities and the Conference Centre.
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- Bahrain Conference Centre- Main Conference Hall
- Boardroom
- Board Room with natural lighting
- Meeting Room
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Meeting Room
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- Harvesters Pub and Terrace - Bar and Lounge
- Harvesters Pub & Terrace - In door bar
- Breakfast buffet - La Mosaique all day dining restaurant
- In-Room Dining
- La Mosaique - All day dining International restaurant
- Waves Seafood Restaurant - Seafood market
- Waves Seafood fine dining restaurant
- Waves Seafood Fine dining restaurant - Bar area
- Spices Indian Restaurant - Open for Business lunch
- Tracks Sports Bar - Pool table
- National Theatre of Bahrain - 3rd largest theatre in Middle East
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New Sportsbook.ag customers: Make your 1st bet, get your 2nd bet free, 100%, winnings paid in cash.Join Now
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JACKSON, Miss. (AP) - Christian Williams turned in a season-high 27 points as Jackson State downed Southwestern Athletic Conference-rival Arkansas-Pine Bluff 82-67 Monday night.
The Tigers (4-14, 3-6) shot 52.8 percent (28 of 53) from the field and knocked down 9 of 22 3-point attempts while winning their third consecutive contest.
Terrell Kennedy paced the Golden Lions (8-13, 7-2) with a career-high 31 points, including an 11-of-14 showing at the foul line. Kennedy hit 10 of 20 from the field, but the rest of Arkansas-Pine Bluff's starters made just 36.7 percent (11 of 30).
Jackson State had 17 assists to the Golden Lions' three and recorded 10 steals, including four by Nelson.
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But who are the real haters?
Religious Left Targets Tea Party "Hate"
John McCain: I was Misled on Bank Bailout – Arizona Republic
Under growing pressure from conservatives and "tea party" activists, Sen. John McCain of Arizona is having to defend his record of supporting the government's massive bailout of the financial system. In response to criticism from opponents seeking to defeat him in the Aug. 24 Republican primary, the four-term senator says he was misled by then-Treasury […]
Sarah Palin's Detractors and Double Standards
David Horowitz and a Guided Tour of the "Ron Paul Revolutionary" Mind
John Fund: Who Rules the Tea Party Empire? – WSJ.com
The Tea Party convention held this past weekend in Nashville wasn't the biggest such gathering (600 people) or the most representative (the ticket price was more than $500) but it did give the national media a pretty good angle on the views and direction of this disparate movement. First, it's clear the group has no […]


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Jurors begin deliberating charges against Boston Marathon bomber
Amanda Knox's murder conviction upheld on appeal
An appeals court in Florence has upheld the guilty verdict against U.S. student Amanda Knox and her ex-boyfriend for the 2007 murder of her British roommate.
Bradley Manning to be sentenced Wednesday at 10 a.m.
A military judge says she'll sentence Army Pfc. Bradley Manning on Wednesday morning in his court-martial at Fort Meade, near Baltimore. He faces up to 90 years in prison for 20 offenses, including six violations of the Espionage Act.
Bradley Manning court martial deliberations
A military judge continues deliberating in the court-martial of an Army private charged with aiding the enemy for giving U.S. secrets to WikiLeaks.
George Zimmerman trial live video: Jury continues deliberationsComment
The jury in George Zimmerman's murder trial continued its second day of deliberations Saturday, weighing whether the neighborhood watch volunteer committed a crime almost a year and a half ago when he fatally shot Trayvon Martin.
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Mutagenesis plays an essential role in molecular biology and biochemistry. It has also been used in enzymology and protein science to generate proteins which are more tractable for biophysical techniques. The ability to quickly and specifically mutate a residue(s) in protein is important for mechanistic and functional studies. Although many site-directed mutagenesis methods have been developed, a simple, quick and multi-applicable method is still desirable.
We have developed a site-directed plasmid mutagenesis protocol that preserved the simple one step procedure of the QuikChange™ site-directed mutagenesis but enhanced its efficiency and extended its capability for multi-site mutagenesis. This modified protocol used a new primer design that promoted primer-template annealing by eliminating primer dimerization and also permitted the newly synthesized DNA to be used as the template in subsequent amplification cycles. These two factors we believe are the main reasons for the enhanced amplification efficiency and for its applications in multi-site mutagenesis.
Our modified protocol significantly increased the efficiency of single mutation and also allowed facile large single insertions, deletions/truncations and multiple mutations in a single experiment, an option incompatible with the standard QuikChange™. Furthermore the new protocol required significantly less parental DNA which facilitated the DpnI digestion after the PCR amplification and enhanced the overall efficiency and reliability. Using our protocol, we generated single site, multiple single-site mutations and a combined insertion/deletion mutations. The results demonstrated that this new protocol imposed no additional reagent costs (beyond basic QuikChange™) but increased the overall success rates.
Site-directed mutagenesis is the cornerstone of modern molecular biology allowing exquisite control of protein sequence. This technique is essential in functional study, genetic engineering, biochemistry and protein engineering. The last category is particularly diverse, including the humanization of antibodies, introduction of new catalytic activities and creation of proteins more suited to biophysical (predominantly structural) characterization. A number of strategies have been developed [1-12] with the QuikChange™ Site-Directed Mutagenesis System developed by Stratagene (La Jolla, CA) probably the most favored. QuikChange™ works by using a pair of complementary primers with a mutation. In a round of PCR cycles these primers anneal to the template DNA, replicating the plasmid DNA with the mutation. The mutant DNA product has a strand break (nick) (Figure 1A). The resulting DNA pool (mutant and parental) is then treated with DpnI to destroy the parental methylated DNA from the newly synthesized unmethylated mutant DNA and transformed into E. coli cells where the nick is ligated by host repair enzymes. The process while extremely useful and simple does have some limitations [13]. As the primers completely overlap, self annealing is quite favorable and care in primer design is required to avoid self pairing competing with template annealing. As the newly synthesized DNA is "nicked", it cannot be used as a template for subsequent amplification in contrast to "normal" PCR (Figure 1A). This constraint leads to a lower PCR amplification efficiency. Although increasing the amount of parental template DNA can help to alleviate this problem it can introduce other complications. As originally developed QuikChange™ cannot introduce multiple mutations, a modified version of the kit (QuikChange™ Multi Site-Directed Mutagenesis kit) has been released and some other adaptations have been reported [14-19]. However, these procedures are either multistep and/or require phosphorylation of oligos and/or enzymatic ligation steps. Addition of short (around 8 base) non-overlapping ends to the primers has been reported and this modification simplified primer design [13]. Deletion or insertion mutations however remain beyond the scope of basic QuikChange™.
Figure 1. Schematic presentations of mutagenesis PCR amplification processes. A) Using the primers designed as recommended in the QuickChange™ protocol. PCR extension fails when primers annealed to newly synthesised "nicked" DNA. B) Using the new primer design to generate single-site mutation, deletion or insertion. C) Using the new primer design to generate double mutations, deletions or insertions. The gray cycles represent the parental plasmid DNA, the cycles of dash lines represent the DNA amplified using the parental DNA as templates while the cycles of gapped dash line are the DNA amplified using the newly-synthesized DNA as templates. Arrows indicate the numbered primers; Triangles indicate the location of the mutations/deletions/insertions; Short bars indicate the "nicks" in the newly-synthesized DNA molecules.
In our structural proteomics lab, site-directed mutagenesis, truncation and deletion mutagenesis are routine methods used by variety of personnel with different expertise in molecular biology. Although most problems with QuikChange™ can be overcome with modification or refinement, we encountered problems in mutation of low GC sequences from cloned Sulfolobus islandicus rudivirus (SIRV) CAG38830 and CAG38833 [20] which we were unable to solve. These problems led us to consider a modification to QuikChange™ that would preserve its simplicity but enhance its efficiency and applicability. Our modified method uses primers containing extended non-overlapping sequences at the 3' end (significantly larger than suggested in [13]) and primer-primer complementary sequences at the 5' end (Figure 2). We used this modified method to make various mutations, including insertions (18 residues) and deletions (25 residues) in a cloned vraR gene of methicillin resistant Staphylococcus aureus (MRSA) [21]. We have also used four primers to create multiple-site mutations, deletions and insertions. This modified procedure has proven to be highly efficient.
Figure 2. Schematic diagram of the primer design for site-directed mutagenesis. Primer designs are shown for site-directed mutation (A), deletion (B) and insertion (C). Triangles, DEL and INS indicate the locations of the mutations, deletion and insertion respectively in the primer sequences.
Primer design
Our new primer design scheme minimized the primer-primer dimerisation and enabled the primers to use the PCR products as the template. The schematic presentation of our new primer design is shown in Figure 2. Each primer pair contains non-overlapping sequences at their 3' end and primer-primer complementary (overlapping) sequences at the 5' end. The non-overlapping sequences are larger (significantly larger than suggested in [13]) than the complementary sequences to make the melting temperature of non-overlapping sequences (Tm no) 5 to 10°C higher than the melting temperature of primer-primer complementary sequences (Tm pp). The mutation sites can be placed either in the complementary region or non-overlapping region. Using the primers designed by our new scheme, the newly synthesized PCR products can be used as the templates in the subsequent PCR amplification cycles, which significantly increased the PCR efficiency and required less template DNA while it is unfeasible in the QuickChange™ or the modification reported previously [13].
Single-site mutation and deletion
Using the primer pair of 3026L/M, 3051L/M, 3327L/M, 3056L/M, FabH2111C/A, FabH2111C/S (Table 1), six single-site mutagenesis reactions were carried out to substitute residues Leu26, Leu51 in CAG38830, Leu27 and Leu56 in CAG38833 by methionines and Cys111 in FabH2 by an alanine and a serine respectively. As controls one primer pair of 3026L/M designed as recommended in the QuikChange™ manual and another designed as described in [13] were tested. Agarose gel electrophoresis showed the synthesis of the full-length plasmid DNA (Figure 3A). DNA quantification showed that amplifications of 3026L/M, 3051L/M and FabH2111C/S produced approximately 1 μg of the synthesized plasmid DNA respectively in each reaction while amplifications of 3327L/M, 3056L/M and FabH2111C/A produced approximately 500 ng respectively. In comparison with the DNA produced using the primer pairs designed by our new scheme the control PCR reactions produced 35 ng for 3026L/M(13) and about 5 ng for 3026L/M(QC). Transformation of E. coli cells with these DNA resulted in a large number of colonies (Figure 3B) indicating a high PCR amplification efficiency. DNA sequencing showed that in each mutagenesis reaction all four transformants contained the desired mutations (Figure 3B). Under these conditions neither control experiment gave colonies which harbored the desired mutants despite five attempts.
Figure 3. PCR amplification for single-site mutagenesis. A) Agarose gel electrophoresis of the PCR reactions indicating the amplification efficiency. The names of the mutants are shown on the top of each lane. B) Transformation and mutation efficiency of each reaction for 3026L/M, 3051L/M, 3327L/M, 3356L/M, FabH2111C/A, FabH2111C/S. Labels for the generated mutants are detailed in the text. 3026L/M(13) and 3026L/M(QC) are control PCR reactions using primer pairs designed as [13] and QuickChange™ protocol respectively.
Table 1. Primers used for mutagenesis
Using the primer pairs of VRARN3, VRARC5 and VRARDHIS (Table 1), we successfully generated a vraR clone with three residues deleted from its N-terminus, a clone with five residues deleted from its C-terminus and a clone with its His tag removed from the N-terminus. PCR amplifications of these mutants again showed high amplification efficiency (Figure 4A). Transformation of E. coli cells with these PCR products produced more than 100 colonies (Figure 4B). Sequencing the plasmid DNA showed that in each reaction all four isolated recombinants contained the desired deletions.
Figure 4. PCR amplification for single-site deletions. A) Agarose gel electrophoresis of the PCR reactions indicating the amplification efficiency. The names of the mutants are shown on the top of each lane. B) Transformation and mutation efficiency for VraRNHISD, VraRC5D and VraRN3D, cloned vraR genes with its N-terminal His tag removal, five residues from the C-terminus and three residues from the N-terminus deleted respectively.
Multiple-site mutations, deletions and insertions
Using two primer pairs 3026L/M and 3051L/M, 3327L/M and 3056L/M (Table 1) double mutant 3026L/M-51L/M of gene CAG38830 and 3327L/M-56L/M of gene CAG38833 were engineered. Agarose gel electrophoresis of the amplified DNA and the colonies produced after transformation are shown in Figure 5. Although partial elongated plasmid DNA fragments were produced in the PCR amplifications, a large number of recombinants demonstrated that our mutagenesis protocol was effective for engineering double mutations. To test the efficiency for multiple-site deletion/insertion in a single step, mutagenesis was carried out using two primer pairs VRARN3 and VRARC5 to remove three residues at the N-terminus and five residues at C-terminus of a clone vraR gene in pDESVRAR for maximum likelihood of crystallisation. A similar experiment with two primer pairs VRARDHIS and VRARIHIS was set up to remove the N-terminal TEV protease cleavable His-tag and insert a C-terminal His-tag in a single reaction. Analysis of the PCR products is shown in Figure 5A. In addition to the full-length PCR products, some partial PCR fragments accumulated in these PCR reactions. These partial amplified DNA fragments were generated with a forward primer and a reverse primer of the downstream primer pair (schematically presented in Figure 1C, primer 1 and primer 4, primer 3 and primer 2) misbridging the "nicks" during the amplification. These partial DNA fragments share some overlap sequences within their primer pairs and could anneal via primer-primer overlapping sequences or act as megaprimers [6] annealed to the template and elongated into the full length plasmid DNA in the subsequent amplification cycles or similar to that described [7]. The synthesis of the full length PCR products for the double-site mutations (3026L/M-51L/M and 3327L/M-56L/M) was more efficient than the multiple-site deletions (Figure 5A). Nevertheless full-length products were produced and transformation of the E. coli cells with these products produced viable transformants. DNA sequencing showed that three of four transformants contained the desired mutations.
Figure 5. PCR amplification for multiple-site mutagenesis. A) Agarose gel electrophoresis of the PCR reactions indicating the amplification efficiency. The names of the mutants are shown on the top of each lane. B) Transformation and mutation efficiency for 3026L/M-51L/M, both Leu26 and Leu51 in CAG38830 substituted by methionines, 3327L/M-56L/M, both Leu27 and Leu56 in CAG38833 substituted by methionines, VraRDNH/ICH, a cloned vraR gene with its N-terminal His tag removed and C-terminal His tag inserted and VraRDN3/DC5, a cloned vraR gene with three residues from the N-terminus and five from the C-terminus deleted. Arrows indicate the partial PCR amplification products.
QuickChange™ site-directed mutagenesis has transformed the ability of labs to carry out site-directed mutagenesis. However its relatively low PCR amplification efficiency can lead to problems and the primer design needs care. Adding additional template alleviates this but can decrease the success rate for obtaining mutants as it leads to a increase of the amount of hemimethylated DNA molecules (newly synthesized strand combined with parental strand) which is resistant to DpnI digestion [22]. Incomplete digestion results in recovery of non-mutated DNA. Modified primer design which destabilizes primer pairs has been proposed [13] to simplify the procedure. However, this and the original method can only use the parental DNA as template in the amplification cycles. This is because the primers either do not bridge or are too short to efficiently bridge the "nick" in the newly synthesized strands. By significantly increasing the non-overlapping region the primers should be long enough to bridge the "nick" and bind to the newly synthesized DNA and thus use it as template (Figure 1B, C) in the same way as normal PCR. We therefore designed primers which contain non-overlapping sequence and primer-primer complementary (overlapping) with a Tm no 5 to 10°C higher than the Tm pp so that the non-overlapping sequences can bridge the "nick" and bind to the newly synthesized DNA efficiently. These primer lengths are within the normal range suggested for QuikChange™ and therefore do not impose any additional costs to the laboratory. The PCR amplification using these primers showed high efficiency and as a result it required less than half the parental template and fewer amplification cycles. The decreased amount of parental DNA has the significant added benefit that decreases the amount of methylated and hemimethylated DNA which needs to be destroyed by DpnI, reducing the potential to recover the parental DNA.
The modified primer design as with that proposed earlier [13] eliminates the problems associated with primer pair self-annealing, and Tm values can be designed as these for conventional PCR [23]. Moreover the restriction upon primer length is also lifted which enhances the utility of the technique. The removal of the primer length limitation allows adjacent multiple mutations to be made in a single step with a pair of mutagenesis primers without any limitation. We have successfully generated double mutations, double deletion and N-terminal deletion and C-terminal insertion mutants simultaneously in a single experiment, demonstrating that this modified method is efficient for multiple site-directed mutagenesis. PCR amplification for multiple-site mutagenesis produced partial DNA fragments with the forward primer in a primer pair (primer 1, Figure 1C) and the reverse primer of the downstream primer pair (primer 4, Figure 1C). These partial elongated DNA fragments annealed each other with their overlap sequences and extended to the full-length plasmid DNA in the subsequent PCR cycles [24] or functioned as the megaprimers in the subsequent cycles as described [11]. There is no distance restriction of the mutation sites. However a long single pair primer should be used for the adjacent multiple mutations. In our experiments the double mutations (3026L/M-51L/M and 3327L/M-56L/M) spanned around 80 base pairs produced more full-length plasmid suggesting that the short partial PCR products could act as megaprimers in the subsequent amplification cycles more efficiently in comparison with the longer PCR fragments (VraRDNH/ICH and VraRDN3/DC5). An extra a few cycles using Tm pp-5 as the annealing temperature can increase the synthesis of the full-length plasmid and placing the mutation sites within the primer-primer overlap sequence can increase the mutation efficiency. Although Tm pp and Tm no of the primer pairs can be variable, a Tm no volume of the primer pairs 5°C to 10°C higher than Tm pp is required for an efficient PCR amplification. Under our PCR conditions, no plasmid concatemers were detected.
Our results demonstrated that the modified protocol is a high efficient method for single site mutagenesis and can be extended to multiple site-directed insertion deletion mutagenesis protocol without any extra steps such as ligation or phosphorylation.
As a result of this research, we have modified the site-directed mutagenesis protocol which increased the efficiency for single- and multiple-site mutations and also enabled facile large single insertions and deletions/truncations in a single experiment, an option incompatible with the standard QuikChange™ protocol or with the protocols reported previously [8,12,13,17,19,25-27]. This single-step protocol utilized a new primer designing scheme and required significantly less parental DNA which facilitated its digestion after the PCR and enhanced the overall efficiency and reliability.
Six single-site mutations and three deletion mutants were generated using this modified protocol. The PCR amplifications with the primers designed by the new scheme revealed high amplification efficiency and required less parental DNA and PCR cycles. Sequence analysis the plasmid DNA revealed that in each mutagenesis reaction all four transformants contained the desired mutations or deletions. Four double-site mutations and two double-site deletions or deletion/insertion were generated using this method. A large number of recombinants demonstrated that our mutagenesis protocol was effective for engineering double mutations, deletions and insertions. Despite the fact that partial elongation products were produced and the syntheses of the full-length plasmid DNA variable, transformation of the resulting products into E. coli cells produced viable transformants. Three of four sequenced transformants contained the desired mutations.
Our new protocol has been used successfully to generate single and multiple-site mutations, deletions, insertion and combined insertion/deletions. The resulted mutants have been successfully used to express the proteins for structure determination (data not shown). The results demonstrated that this new protocol imposed no additional reagent costs but increased the overall success rates.
Plasmid pDESTSIRV30, pDESTSIRV33 expressing the SIRV proteins (CAG38830 and CAG38833), pDESTAVRA expressing MRSA vraR protein (CAG40961) and pDESTFaBH2 expressing Pseudomonas aeruginosa FaBH2 protein (AAG06721)[28] were constructed using a modified Gateway technology with an N-terminal TEV protease cleavable His tag [29]. All the plasmids were propagated in DH5α E. coli cells (Stratagene, La Jolla) and plasmids were prepared using Qiagen miniprep kits (Qiagen, Germany). Pfu DNA polymerase, DpnI restriction enzyme are provided with QuikChange™ kit purchased from Stratagene, additional Pfu DNA polymerase was purchased from Promega when required. All the primers were synthesized by Eurogentec and simply purified by SePOP desalting. The melting temperature was calculated as Tm = 81.5 + 16.6(log([K+]/(1+0.7 [K+])) + 0.41(% [G+C]) – 500/(probe length in base) – 1.0(%mismatch) [30]. The Tm pp and Tm no were calculated for each primer. All primers and their Tm no and Tm pp are detailed in Table 1. PCR cycling was carried out using a Px2 thermal cycler (Thermo Electro Cooperation).
For single-site mutation, deletion or insertion, the PCR reaction of 50 μl contained 2–10 ng of template, 1 μM primer pair, 200 μM dNTPs and 3 units of Pfu DNA polymerase. The PCR cycles were initiated at 95°C for 5 minutes to denature the template DNA, followed by 12 amplification cycles. Each amplification cycle consisted of 95°C for 1 minute, Tm no -5°C for 1 minute and 72°C for 10 minutes or 15 minutes according to the length of the template constructs (about 500 bp per minute for Pfu DNA polymerase). The PCR cycles were finished with an annealing step at Tm pp-5 for 1 minute and an extension step at 72°C for 30 minutes. The PCR products were treated with 5 units of DpnI at 37°C for 2 hours and then 10 μl of each PCR reactions was analyzed by agarose gel electrophoresis. The full-length plasmid DNA was quantified by band density analysis against the 1636-bp band (equal to 10% of the mass applied to the gel) of the DNA ladders. An aliquot of 2 μl above PCR products, the PCR products generated using QuickChange™ or generated as described in [13] was transformed respectively into E. coli DH5α competent cells by heat shock. The transformed cells were spread on a Luria-Bertani (LB) plate containing antibiotics and incubated at 37°C over night. The number of colonies was counted and used as an indirect indication of PCR amplification efficiency. Four colonies from each plate were grown and the plasmid DNA was isolated. To verify the mutations, 500 ng of plasmid DNA was mixed with 50 pmole of T7 sequencing primer in a volume of 15 μl. DNA sequencing was carried out using the Sequencing Service, University of Dundee. For multiple site-directed mutations, deletions and insertions, the PCR was carried out in 50 μl of reaction containing 10 ng of template, 1 μM of each of the two primer pairs, 200 μM dNTPs and 3 units of Pfu DNA polymerase. The PCR cycles, DNA quantification, transformation and mutation verification were essentially the same as described above.
Authors' contributions
HL designed the experiments, carried out the practical work and drafted the manuscript. JHN was involved in the research discussion and helped to finalise the manuscript. All authors read and approved the final manuscript.
This work was carried out at Scottish Structural Proteomics Facility, which is funded by BBSRC, Scottish Funding Council (SFC) and the University of St. Andrews.
Kunkel TA: Rapid and efficient site-specific mutagenesis without phenotypic selection.
Proc Natl Acad Sci USA 1985, 82:488-492. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Cormack B: Introduction of a point mutation by sequential PCR steps.
Ishii TM, Zerr P, Xia XM, Bond CT, Maylie J, Adelman JP: Site-directed mutagenesis.
Methods Enzymol 1998, 293:53-71. PubMed Abstract
Ling MM, Robinson BH: Approaches to DNA mutagenesis: an overview.
Anal Biochem 1997, 254:157-178. PubMed Abstract | Publisher Full Text
Vandeyar MA, Weiner MP, Hutton CJ, Batt CA: A simple and rapid method for the selection of oligodeoxynucleotide-directed mutants.
Gene 1988, 65:129-133. PubMed Abstract | Publisher Full Text
Sarkar G, Sommer SS: The "megaprimer" method of site-directed mutagenesis.
Biotechniques 1990, 8:404-407. PubMed Abstract
Klock HE, Koesema EJ, Knuth MW, Lesley SA: Combining the polymerase incomplete primer extension method for cloning and mutagenesis with microscreening to accelerate structural genomics efforts.
Proteins 2008, 71:982-994. PubMed Abstract | Publisher Full Text
Chapnik N, Sherman H, Froy O: A one-tube site-directed mutagenesis method using PCR and primer extension.
Anal Biochem 2008, 372:255-257. PubMed Abstract | Publisher Full Text
Kumar R, Rajagopal K: Single-step overlap-primer-walk polymerase chain reaction for multiple mutagenesis without overlap extension.
Anal Biochem 2008, 377:105-107. PubMed Abstract | Publisher Full Text
Li X, Qiu Y, Shen Y, Ding C, Liu P, Zhou J, Ma Z: Splicing together different regions of a gene by modified polymerase chain reaction-based site-directed mutagenesis.
Anal Biochem 2008, 373:398-400. PubMed Abstract | Publisher Full Text
Tseng WC, Lin JW, Wei TY, Fang TY: A novel megaprimed and ligase-free, PCR-based, site-directed mutagenesis method.
Anal Biochem 2008, 375:376-378. PubMed Abstract | Publisher Full Text
Li J, Li C, Xiao W, Yuan D, Wan G, Ma L: Site-directed mutagenesis by combination of homologous recombination and DpnI digestion of the plasmid template in Escherichia coli.
Anal Biochem 2008, 373:389-391. PubMed Abstract | Publisher Full Text
Zheng L, Baumann U, Reymond JL: An efficient one-step site-directed and site-saturation mutagenesis protocol.
Nucleic Acids Res 2004, 32:e115. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Mikaelian I, Sergeant A: A general and fast method to generate multiple site directed mutations.
Nucleic Acids Res 1992, 20:376. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Jensen PH, Weilguny D: Combination primer polymerase chain reaction for multi-site mutagenesis of close proximity sites.
J Biomol Tech 2005, 16:336-340. PubMed Abstract | PubMed Central Full Text
Young L, Dong Q: TAMS technology for simple and efficient in vitro site-directed mutagenesis and mutant screening.
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Biotechniques 2000, 28:196-198. PubMed Abstract
Wang W, Malcolm BA: Two-stage PCR protocol allowing introduction of multiple mutations, deletions and insertions using QuikChange Site-Directed Mutagenesis.
Biotechniques 1999, 26:680-682. PubMed Abstract
Post MA: Site directed mutagenesis by ligation-during-amplification: Change-IT Multiple Site Directed Mutagenesis Kit.
Kessler A, Brinkman AB, Oost J, Prangishvili D: Transcription of the rod-shaped viruses SIRV1 and SIRV2 of the hyperthermophilic archaeon sulfolobus.
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J Mol Biol 1977, 115:525-538. PubMed Abstract | Publisher Full Text
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Nucleic Acids Res 1989, 17:8543-8551. PubMed Abstract | Publisher Full Text | PubMed Central Full Text
Aiyar A, Xiang Y, Leis J: Site-directed mutagenesis using overlap extension PCR.
Methods Mol Biol 1996, 57:177-191. PubMed Abstract
Hansson MD, Rzeznicka K, Rosenback M, Hansson M, Sirijovski N: PCR-mediated deletion of plasmid DNA.
Anal Biochem 2008, 375:373-375. PubMed Abstract | Publisher Full Text
Ho SN, Hunt HD, Horton RM, Pullen JK, Pease LR: Site-directed mutagenesis by overlap extension using the polymerase chain reaction.
Gene 1989, 77:51-59. PubMed Abstract | Publisher Full Text
Seyfang A, Jin JH: Multiple site-directed mutagenesis of more than 10 sites simultaneously and in a single round.
Anal Biochem 2004, 324:285-291. PubMed Abstract | Publisher Full Text
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Wetmur JG: DNA probes: applications of the principles of nucleic acid hybridization.
Crit Rev Biochem Mol Biol 1991, 26:227-259. PubMed Abstract


text:
Chop't CEO wants to take brand national
Having turned his trendy chain of New York-based salad restaurants into a success, Chop't CEO Nick Marsh is determined to go national. The trick will be convincing middle America that...
or email firstname.lastname@example.com
Published: July 27, 2014 | 5:18am
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text:
Hemorrhoids! How do you know?
If you use a mirror, you should be able to see a swelling where the hemorrhoid is. Due to constipation though, you could have just caused some inner bleeding which should clear up soon. If you can't see a hemorrhoid and the bleeding doesn't stop in the next day or two (or gets worse), call your doctor.
Sounds like your bowels are just stressed. You might try taking magnesium powder on top of whatever fiber or other products you use to stay regular. It is a huge aid in digestion and circulation; and helps to prevent and heal hemorrhoids.
Bleeding is from an internal hemorroid. But they say that it can be something else. Meaning the blood could be from another medical problem. So if your scared get it checked out or ask your ob.
Caitlyn 9/01/10
Aiden 12/12/14
Cautiously expecting our little bean 2-21-13.
Mine was irritated right around the beginning of this pregnancy...it bled every time I had a bowel movement, but other than that, it didn't really hurt or anything. I didn't even bring it up, but apparantly it was bad enough that my doctor mentioned it during my pap a couple weeks later. There was a decent amount of blood (enough to wipe several times and still have blood and then still leak into my undies) but it finally went away about 2 months into my pregnancy. It hasn't been an issue anymore this time around and my doctor guessed it was probably just leftover from last pregnancy and to find some OTC medications if it was bothering me at all.
Proud Mommy To My 3 Kiddos! 2 Boys, 1 Girl.
Try and take mineral oil. It has no flavor and it works for constipation.
If you feel around down around your bumhole and you feel things which feel like little (uninflated water balloon sized) bags of squishiness then you have a hemorroid. I would just assume in your case that the giant BMs are tearing you a little. I had that problem from the age of about 6 years old, but I didn't have hemorroids until after I gave birth to #1. The went away. Then they came back when i was preggo with #2. They went down afterwards and almost went away. When I had #3 they came back and brought friends. I bet I have close to a dozen back there. They've never shrunk and they explode and bleed like crazy every so often. Enjoy motherhood!
Crystal wife to Ryan 6-12-1999
Mommy to B 9/01/02, L 5/19/05, C 4/13/07, S 2/17/13,
Q 3/16/14 and G 5/5/15


text:
the holiday and travel guide to the UK
Greenock Tourist Information
> Newark Castle
Visit Their Website
Newark Castle
Post Code : PA14 5NH
Telephone : 01475 741858
Admission Charge
Stunning Jacobean castle on the banks of the river Clyde.
The beautiful stone castle was built in the 15th century with major additions in the 16th century. The fine stonework of this remarkably complete building includes some wonderful turrets and gables and a large and unusual dovecot. The settling is also of interest, amongst the Clydebank industrial landscape and shipyards that once completely hid it from view.
Other Tourist Attractions Near Newark Castle
Geilston Garden - Dumbarton (2.24 miles)
Finlaystone Country Estate - Greenock (2.33 miles)
McLean Museum and Art Gallery - Greenock (3.62 miles)
Scottish Maritime Museum - Dumbarton (4.35 miles)
Dumbarton Castle - Dumbarton (4.46 miles)
Greenock Cut Visitor Centre - Inverkip (5.04 miles)
The Waverley - Helensburgh (5.18 miles)
Loch Lomond Outlet Centre - Alexandria (5.39 miles)
Maid of the Loch - Balloch (5.95 miles)
Loch Lomand Shores - Loch Lomond (5.97 miles)
All distances are straight-line distances.
How to get to the Newark Castle
Location Map of Newark Castle
Nearest Railway Station
Opening Times
1 Apr-30 Sept: Daily 09:30-17:30
Have You Visited the Newark Castle ?
Add Your Quick Review or Tip on Visiting the Newark Castle
Hotels near Newark Castle
Premier Inn Greenock - Greenock
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>> See More Accommodation near Newark Castle
Featured Budget Hotel
Premier Inn Greenock
The Point, 1/3 James Watt Dock, James Watt Way, Greenock
More Details and Book Online
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Little Arches - Lochwinnoch
Sleeps 2
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text:
The 3D-quality
View Single Post
May 10 2013, 02:39 AM
Location: Victoria, Australia
Re: The 3D-quality
I'm not a fan of 3D in movies at all, but it was done really well here. I saw Ghost Rider 2 in 3D (not by choice) and it was awful - maybe a bit of extra depth to a few scenes, but virtually no difference to seeing it normally.
There was a huge difference in this, and it looked bloody spectacular while not constantly throwing things in your face. Just little things like ash floating around looked great, and the first warp effect was simply beautiful.
The only bit which bugged me was a bar scene where there were some really blurry objects at the 'front', which was distracting.
Kim: I'm detecting some weird technobabble.
Janeway: A possibly dangerous anomaly that we know nothing about?
Kim: Yeah. I suppose we should steer clear.
Janeway: Ha! Good one, Ensign. Take us in, Mr. Paris.
View Public Profile
Send a private message to donners22
Find More Posts by donners22


text:
ODT Online
ODT /directory
Blackadder star Sir Tony Robinson has a cunning plan
for Dunedin.
The English actor, who rose to prominence with his portrayal
of Baldrick in the British comedy Blackadder, will
visit Dunedin later this month to film a new series for the
History Channel.
The show - titled Tony Robinson's Tour of Duty - will
have Sir Tony exploring how war affected New Zealanders and
Australians and visiting towns in the two countries to meet
the relatives and friends of those who fought in battle.
Sir Tony will travel to Dunedin and while in the city will
host a community event at Toitu Otago Settlers Museum on
March 30 from 12.30pm-5.30pm.
Members of the public are welcome to attend.
A name, residential address, and (preferably residential) telephone number is required from readers who comment on ODT Online. These details will not be visible to site visitors.
Login or Register
to add your business to the ODT/directory. Quick link to
more roadtests & reviews
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Opinion? Have your say - Login or Register
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event or notice
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Bookmark ODT
Make ODT my
Otago Daily Times print and digital edition, The
Star - Dunedin, The News - Central Otago, The
Ensign - Gore, Southland Express -
Invercargill, The Courier - Timaru, Queenstown Times - Queenstown, Mountain Scene -
Queenstown, Extra! schools
publication, Property
Times South Canterbury


text:
Robert Childs, senior director, National Defense University's iCollege
Click to hear the interview
Jump to main content.
Monday - 4/26/2010, 4:44pm EDT
Click to hear the interview
Cybersecurity Update - Tune in weekdays at 30 minutes past the hour for the latest cybersecurity news on The Federal Drive with Tom Temin and Jane Norris (6-10 a.m.) and The Daily Debrief with Chris Dorobek and Amy Morris (3-7 p.m.). Listen live at FederalNewsRadio.com or on the radio at 1500 and 820 AM in the Washington, D.C. metro area.


text:
Gum Disease Center - Grand Island, NE
WebMD Physician Directory
Grand Island, Nebraska
Gum Disease
What is gum disease (gingivitis)?
Gum disease, or gingivitis, is inflammation of the tissues surrounding and supporting the teeth and is most commonly a result of poor dental hygiene. Gingivitis is a very common condition and varies wide...
Other Related Gum Disease Articles
Emergency Contact for Grand Island
Nearby Grand Island Hospitals *
Saint Francis Medical Center
VA Medical Center
Memorial Hospital
Howard County Medical Center
Litzenberg Memorial County Hosp
Mary Lanning Memorial Hospital
Hasting Regional Center
Henderson Healthcare Services
*Provider Directory Terms of Use:
The WebMD 'Provider Directory' is provided by WebMD for use by the general public as a quick reference of information about Providers. The Provider Directory is not intended as a tool for verifying the credentials, qualifications, or abilities of any Provider contained therein. Inclusion in the Provider Directory does not imply recommendation or endorsement nor does omission in the Provider Directory imply WebMD disapproval.


text:
TheSpread is Pleased to Welcome GTBets as Our Newest Sportsbook Sponsor!
Limited Time Offer - Free $20 Bet Just for Signing Up! Don't Miss Out on Free Money, This Offer Will Expire Soon Click Here to Get Your Free $20 Bet
- Index
- » Football Discussion
- » Thursday Football Service Plays
Thursday Football Service Plays
Thursday Football Service Plays
Lou Diamond.
NO Saints and ARI Cardinals
Take Over
With coaches tipping their hands, some Pre-Seaon games can be just as strong, if not stronger, than regular season games. Find out why I have 3 easy NFL winners for today with my SPOT-ON analysis! Check it out! Both teams are beefing up their offenses and have some quality pre-season qb's that should give us opportunities to score all 4 quarters. Look for a high scoring affair here tonight.
Re: Thursday Football Service Plays
Baltimore at New England
Pick: Baltimore +5
It is going to be difficult psychologically for bettors to look at a New England game and resist them as just a mere 5 point favorite at home. What we must do however is keep this in perspective. This is an exhibition game! The logo on the helmets and regular season success and failure do not come into play here. But the oddsmakers are forced by the hand of the betting public to consider a few extra points. Even with that said over 60% have already pulled the trigger for the Pats in this one. The Pats opened the season in 2007 scoring 30+ points in their first eight regular-season games. A great start, right? Consider however they did not reach 30 in a single exhibition game. Brady threw seven passes in the opener last season. The result was 10 total points scored and a Patriot loss. Their's no doubt this offense will turn it on come September, but the preseason is a different animal all together. The Patriots have been an elite team for years,but over the last three exhibitionseasons they have been favored by 4 or more points three times and have not covered a single one. In contrast, we have the Baltimore Ravens with a first year coach in John Harbaugh. What does that mean? First year coaches are new for a reason - their teams were bad last season - bad enough to fire their head man. New coaches come in for one reason - to change things. They want to win from the start, more than entrenched coaches, and especially more than coaches of teams so good that have nothing to prove to anyone (i.e. the Pats). These first-year coaches want to change the culture of losing and as such, they put more of an emphasis on winning in the preseason. Does Harbaugh want to win here? Yes! Does Bellichick? He could really care less. The Patriots have dropped two of their last three ehibition home openers outright. We will back the more motivated Ravens here to keep this one close, or even steal one.
Re: Thursday Football Service Plays
FAO Sports
Kansas City and Chicago,
Take Chicago
This is more of a play against K.C. then one for Chicago.Kansas City is 0-3 ATS and SU, the first game of the preseason. They have lost the first game of the preseason by an average of 8.33 PPG.Chicago is 1-1-1 in the first game of the preseason in the last 3 years.
Take Chicago
Re: Thursday Football Service Plays
Paul Bovi
NO Saints and ARI Cardinals
Take Under
It will be Tyler Palko, Mark Brunell and Travis Lulay getting most of the snaps for the Saints while coach Whisenhunt looks for his inaugural preseason win after last years.5? 0-4, though several were close. The Saints are sitting the bulk of the offensive talent inclding Deuce, Devery and Marques and will pay more close attention to the defensive side here as that has been their Achilles heel. Zona in the desert gets it 16-13 Under
Re: Thursday Football Service Plays
CLEVELAND BROWNS (-3) VS. New York Jets: The Jets are the play here as the QB question surrounding Gang Green will force coach Eric Mangini to play both Chad Pennington and Kellen Clemens for an extended amount of time here. Cleveland will play Brady Quinn a bunch and this will represent his first extended action as his rookie season was spent holding the clipboard. Jets are also 15-5 ATS in their last 20 preseason openers. THE PICK: New York Jets (+3)
NEW ENGLAND PATRIOTS (-5) VS. Baltimore Ravens: The Ravens will start Kyle Boller at QB which is never a good thing even in preseason. The Pats should come out fast as this is their first action since losing Super Bowl 42 in shocking fashion. Pats are also 32-19 ATS in their last 51 preseason openers when giving less than 5.5. THE PICK: New England Patriots (-5)
CHICAGO BEARS (-3) VS. Kansas City Chiefs: The Bears should beat up on the Chiefs here as KC breaks in a host of rookies on both sides of the ball and still have QB Brodie Croyle finding his way. Bears are looking for who will step up as QB and so Rex Grossman and Kyle Orton will look to open some eyes with their showing. THE PICK: Chicago Bears (-3)
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- » Thursday Football Service Plays
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text:
UNR's Raggio Building Closed After Water Line Break
Updated: 10/14/2014 - The Raggio building on the University of Nevada, Reno campus is closed for classes until at least the rest of the week.
Updated: 10/14/2014 - The Raggio building on the University of Nevada, Reno campus is closed for classes until at least the rest of the week.
Posted: 10/14/2014 - A 28-year-old man who was hit and killed while in a crosswalk has been identified.
Posted: 10/14/2014 - Reno police have identified the woman who was hit and killed on Peckham Lane earlier this month.
Updated: 10/14/2014 - The National Transportation Safety Board is still investigating the crash of a single-engine Cessna 170 and an unidentified experimental aircraft in midair above a dry lake bed.
Posted: 10/14/2014 - Reno police have arrested one person in connection with a stabbing Tuesday morning, but are still looking for the attacker in another.
Updated: 10/14/2014 - A northern Nevada teen is using her incredible talent to raise money for local charities.
Posted: 10/14/2014 - For many, fall is a special time of year for football and colorful leaves, but for people with allergies, the season can be miserable.
Updated: 10/13/2014 - The rise in cyber crime has business and government scrambling to catch up. In the process, jobs are opening up in cyber security.
Posted: 10/13/2014 - A defense lawyer says a 24-year-old Reno man accused of killing and dismembering several dogs will go forward with a preliminary hearing if a plea deal can't be worked out with Washoe County prosecutors.
Posted: 10/13/2014 - The Regional Transportation Commission of Washoe County has completed a corrective maintenance project on Plumas Street. The street is now fully open.
Updated: 10/13/2014 - A 71-year-old man was killed after his vehicle struck a tree and rolled over Friday morning.
Posted: 10/11/2014 - The day has come. Zombies have taken over Virginia City. Nearly 1,000 people ran for their lives to escape the haunted Comstock Sunday afternoon. Could you survive?
Posted: 10/11/2014 - A state panel has granted tax breaks to Amazon.com for its new distribution center in Reno.
Posted: 10/11/2014 - Firefighters gained the upper hand overnight on a wildfire along Northern California's Interstate 80 east of Sacramento that threatened 1,000 homes.
Updated: 10/11/2014 - NV Energy is reporting that power has been restored to most customers.
Posted: 10/10/2014 - Friends and family said goodbye to 100-year-old Carson City veteran James Sorrentino Friday.
Updated: 10/15/2014 - The third annual Red Shoe Gala helps hundreds of families who stay at the Ronald McDonald house while their kids are in the hospital.
Updated: 10/10/2014 - An initial frenzied filing for Nevada's first same-sex marriage licenses followed by quickie late-night ceremonies is giving way to business almost as usual in the self-proclaimed wedding capital of the world.
Updated: 10/10/2014 - We are not expecting a flood right now but get ready; the big one is coming.
Posted: 10/10/2014 - The Nevada Department of Transportation is announcing that a project to repave and enhance the Mt. Rose Highway will complete one full season ahead of schedule.
Updated: 10/10/2014 - A 16-year-old Gardnerville girl who went missing a week ago has been found, according to the Douglas County Sheriff's Office
Updated: 10/10/2014 - A Carson City man goes all out decorating his home for Halloween and offers tips for building your own haunted house.
Posted: 10/10/2014 - Sparks police are asking for help finding two people who took a purse from a woman.
Updated: 10/10/2014 - It was the time to get all gussied up at the north shore Thursday night for the 2014 Veterans Ball honoring World War II and Korean War Veterans. The ball was hosted by the Incline Village and Crystal Bay Veterans Club.
Updated: 10/10/2014 - The Reno City Planning Commission voted unanimously to expand the border of the University of Nevada Regional Center Plan Thursday night.


text:
Up late so you'll probably skip class
Life is a test so before the night pass
Get right, get right
from J. Cole – Work Out Lyrics on Genius
Continuing on about his college girls who stay up late with Cole when he's visiting. Cole employs some of his clever wordplay with "test," "pass," and "get right," all tying in with the college theme. Get right can mean get high or drunk or just getting in the right mood.
To help improve the quality of the lyrics, visit J. Cole – Work Out Lyrics and leave a suggestion at the bottom of the page


text:
National Sports
Lockout getting expensive...KHL games to air in statesNEW YORK (AP) - The financial losses are starting to pile up as a result of the NHL lockout.
And yesterday the league made that public.
In speaking to reporters after talks between the NHL and the players association finished up for the day, Deputy Commissioner Bill Daly estimated that the league has lost $100 million in revenues from the canceled preseason.
There are no further talks scheduled, increasing speculation that regular-season games could be lost, with an announcement sometime this week. The season was slated to begin Oct. 11.
Meanwhile, the Russian Kontinental Hockey League says it will broadcast its games in the United States on ESPN3 during the NHL lockout.
The Russian-based league announced on its website Tuesday that it plans to air five games in the next week on ESPN's the online streaming service. But an ESPN spokesman says it's not a done deal.
See other NationalSports news:Michael Sam Signs Two-Year Deal with Montreal Alouettes
NHL Playoffs: Ducks Edge Blackhawks, Take 2-1 Series Lead
FIFA Women's World Cup: Complete Schedule
Hendricks' 5-hitter, Bryant's 2-run homer carry Cubs, 3-0
Big days for deGrom, Duda lead Mets over Cardinals 5-0


text:
Ashton Kutcher Infuriates Mila Kunis!
Ashton Kutcher has been infuriating Mila Kunis with their first child, Wyatt.
Ashton has never gotten the joy of having his own baby before and only helped out with his ex-wife's grown children. Now that he's a new father for the first time, he's been non-stop with the cameras!
Can you really blame him?
Sources close to the situation have revealed that Mila is fed up with Ashton recording every possible moment in Wyatt's life. A source said, "Ashton wants to document every second of her life, and Mila's at wit's end. She told him, 'Honey, you don't have to record every single burp – it's getting ridiculous!'"
The spy added, "But Ashton's SO crazy about his first child, he just can't stop himself. And it's kind of sweet."
Now if he would only share some of these lovely baby pictures with the rest of the world, that would be great.
Source, Photo Credit: Fame/Flynet
Filed under Ashton Kutcher, Mila Kunis | No Comments


text:
The Leadership Essentials program hosts information activities, including happy hours and networking events, leading up to the fall application deadline.
Read more about Leadership Essentials and meet the class of 2014.
There are no upcoming events at this time.
There are many more ways to learn more about the program. To speak to a Loyola representative, contact Lori Fuchs, program manager at firstname.lastname@example.com or call 410-617-7639. Through Ms. Fuchs you may arrange to speak to an alum to learn about their experience and the impact the program has had on their career.
Information Sessions
Info Session and Class Test Drive — Columbia. Tues., June 2
Graduate Business Programs Info Session — Timonium. Sat., June 6
Master of Accounting Virtual Session — Online. Wed., June 10
Emerging Leaders MBA Virtual Session — Online. Thurs., June 11
All Programs Virtual Session — Online. Tues., June 23
Meet the Program Managers — Columbia. Wed., July 15
Info Session and Class Test Drive — Timonium. Thurs. July 23
>> more information sessions


text:
The King of Fighters XIII
Saiki has some of the best themes in the whole series
Fate, diabolosis, the man who rules time...they're all epic
Agreed. Has a good ambiance.
Tame a Badboy or Arashi no Saxophone 5 are my favorites.
"You interfered with a well-laid plan."
"Yeah, well you can stick you well-laid plan up your well-laid a**"
Yeah those two are actually my other favorites too
One thing I'm having trouble deciding is which version of "fate" I like better
the original is more epic, dramatic, and sinister. The arranged version is more of a groove and has a cocky swag feel to itl
One of the most beautiful songs ever recorded
Chizuru Kagura
Greatest Shows of All Time.
Avatar: The Last Airbender, SWAT Kats, Sailor Moon, Tetsuwan Atom 2K3, Transformers, Spiderfriends
TheArcade posted...
Chizuru Kagura
You mean "Fairy", and its remixes/arranged tracks.
"The Alternate Truth is yet to be Found" - XBL/PSN/YT/TW: NeoStrayCat (=^_^=)
From: TheArcade | Posted: 1/9/2013 2:15:18 PM | #005
Chizuru Kagura
Fairy is pretty good, yeah
That, Saiki's themes, and the themes oorochi and the orochi temas are the best for me
One of the most beautiful songs ever recorded


text:
Milani Brow Fix Brow Kit
146 reviews
90% would repurchase
Package Quality: 3.9
Price: $$
Package Quality: 3.9
Price: $$
Not tested on animals
on 8/18/2014 2:46:00 PM
Age: 19-24
Skin: Combination, Fair, Not Sure
Hair: Brunette, Other, Other
Eyes: Brown
This brow powder is so good, really natural-looking, good pigmentation.
The highlight shade is quite pigmented and beautiful as well.
Its so cheap, and comes with little applicators which was certainly a nice thought.
on 8/15/2014 8:21:00 AM
Age: 19-24
Skin: Normal, Fair, Not Sure
Hair: Brunette, Curly, Other
Eyes: Blue
I love this product and I have re-purchased it.. It is very natural looking and stays all day. This is the only drug store brow product that I like.
on 8/14/2014 9:05:00 PM
Age: 44-55
Skin: Normal, Fair, Not Sure
Hair: Blond, Other, Other
Eyes: Blue
Excellent brow kit, especially for the price. This is definitely my most successful drugstore makeup purchase.
Your success will probably vary according to whether the colors happen to suit you. All I can say is, after trying this I'm glad I didn't invest in anything more expensive! I used the lighter shade until I colored my hair and now I use the darker one. The powder glides right onto the surface of my brow hairs and then stays all day! It's great.
The highlighter is too frosty for me to use up by the brow, but I use it to brighten the inner corners of my eyes.
on 8/13/2014 3:29:00 PM
Age: 56 & Over
Skin: Combination, Medium, Neutral
Hair: Other, Other, Other
Eyes: Hazel
So happy to see the positive reviews here! I'll just echo them. Brow powder is much more natural and long lasting on me than pencil. Nice to have two shades, and I like he highlighter as well. Great buy!
on 8/11/2014 11:35:00 AM
Age: 25-29
Skin: Combination, Fair-Medium, Not Sure
Hair: Brunette, Straight, Fine
Eyes: Brown
Best natural eyebrow "powder" ever! Unless you like to spend a ton of money in Anastasia products, which would be pointless, considering I have Anastasias Pomade, and I haven't reached for it since buying Milani's Brow Fix. Now, if you like bold, sharply lined, super duper dramatic brows, this may not be the best option. However, this gives an absolutely natural looking brow, and the highlight compliments wonderfully. My brows are naturally thin and unfortunately sparse due to my hypothyroidism, so this product does amazing things to my brows. I highly recommend, and will continue to purchase!
on 8/7/2014 4:37:00 PM
Age: 18 & Under
Skin: Combination, Fair-Medium, Not Sure
Hair: Brunette, Other, Other
Eyes: Hazel
I don't normally fill in my brows, I hadn't found a product that made them look better than they originally were until I found this. My eyebrows are pretty dark but I use the shade "Light" to fill them in, and it works wonderfully. I can use this as an eyeshadow as well: The Hi-Lite shade works to brighten up the inner corner of
Your eyes, the Light shade to fill in eyebrows and use in crease, and the darker shade to use on the top lash line and bottom lash line. It has perfect colors, it's well pigmented, and lasts all day. I will be repurchasing.
on 7/30/2014 11:18:00 PM
Age: 18 & Under
Skin: Oily, Fair-Medium, Not Sure
Hair: Blond, Other, Other
Eyes: Blue
LOVE THIS! My brows are fairly light, but I can mix the colors to achieve the perfect shade (color 01 Light) Also looks very natural! A must have!
on 7/25/2014 1:23:00 AM
Age: 25-29
Skin: Combination, Fair-Medium, Not Sure
Hair: Brown, Straight, Fine
Eyes: Brown
I had tried some of anastasia stuff before but I just dont like how harsh it is. THIS product on the other hand is so lovely esp when using moisturizer right before hand. I wish they made that little brush a LIFE size one! I highly recommend it ! I dont know why but milani seems to know how to make BROW STUFF RIGHT!
on 7/20/2014 3:00:00 AM
Age: 19-24
Skin: Normal, Tan, Not Sure
Hair: Brunette, Other, Other
Eyes: Brown
I LOVE IT !! really easy to apply and looks natural on my eyebrow. I use the darkest one and it blend well with my eyebrows.
on 7/13/2014 9:19:00 AM
Age: 25-29
Skin: Very Dry, Medium Brown, Not Sure
Hair: Brown, Other, Other
Eyes: Brown
By no means am I a brow expert, but I really love this kit. It makes my brows look natural and well-defined, and it is easy to use. Before I bought this kit, my brows never looked very well done, I had the ELF kit, and I also had tried brown shadow. I love mixing the two colors, and I definitely use the highlighter to define under my brows. I tend to use this product ink combination with the Milani pencil with the spoolie on the end and I get great, natural looking results. I've read the comments about the red pigmentation in the kit, but I have reddish tones in my hair so it works perfectly for me.
Popular Brows Products
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text:
Geographic Range
Honduras south to Peru, Bolivia and Paraguay.
- Biogeographic Regions
- neotropical
Specimens have been collected along stream beds and other moist areas, but can also be found in dryer areas. Animals were found equally in open and forested habitat.
- Terrestrial Biomes
- forest
- rainforest
Physical Description
P. hastatus is one of the larger American bats with a body length of 100 to 130mm and a wing span of 455 mm. Its coloration is dark brown; the ventral surface is paler with an orange tinge. It has a well developed nose leaf, widely separate ears, and short tail, and males have a well developed throat sac. The lower lip has a "V" shaped grove with numerous protuberances.
- Other Physical Features
- endothermic
- bilateral symmetry
Phyllostomus hastatus may be monoestrus (one reproductive bout per year) or polyestrus (multiple reproductive bouts in a year); the pattern may be geographically dependent. Lactating females have been found throughout the year. They generally have one offspring at a time. Gestation about 4 months.
- Key Reproductive Features
- gonochoric/gonochoristic/dioecious (sexes separate)
- sexual
- Average lifespan
Status: captivity
- 18 years
- AnAge
- Average lifespan
Roosts in colonies of 10 to 100 individuals. Roosts have been found in hollow trees, termite nests, caves, and thatched roofs. Males may defend a group of females within a colony, forming temporary harems of up to 30 females per male.
- Key Behaviors
- motile
Communication and Perception
Food Habits
This species is omnivorous, eating primarily vertebrates, flowers and pollen. Radio tracking studies found that individuals will travel up to 5km to feeding sites each night.
Economic Importance for Humans: Positive
Food habits involve the removal of pest insects and also aid in pollination of some crops.
Economic Importance for Humans: Negative
Eat some fruits (bananas) that humans would consume.
Conservation Status
No special status, but habitat destruction may be a concern.
- IUCN Red List
Least Concern
More information
Anna Bess Sorin (author), Biology Dept., University of Memphis.
- Neotropical
living in the southern part of the New World. In other words, Central and South America.
- bilateral symmetry
having body symmetry such that the animal can be divided in one plane into two mirror-image halves. Animals with bilateral symmetry have dorsal and ventral sides, as well as anterior and posterior ends. Synapomorphy of the Bilateria.
- chemical
uses smells or other chemicals to communicate
- endothermic
animals that use metabolically generated heat to regulate body temperature independently of ambient temperature. Endothermy is a synapomorphy of the Mammalia, although it may have arisen in a (now extinct) synapsid ancestor; the fossil record does not distinguish these possibilities. Convergent in birds.
- forest
forest biomes are dominated by trees, otherwise forest biomes can vary widely in amount of precipitation and seasonality.
- motile
having the capacity to move from one place to another.
- native range
the area in which the animal is naturally found, the region in which it is endemic.
- rainforest
rainforests, both temperate and tropical, are dominated by trees often forming a closed canopy with little light reaching the ground. Epiphytes and climbing plants are also abundant. Precipitation is typically not limiting, but may be somewhat seasonal.
- sexual
reproduction that includes combining the genetic contribution of two individuals, a male and a female
- tactile
uses touch to communicate
Redford, K.H. and J.F. Eisenberg. 1992. Mammals of the Neotropics. University of Chicago Press: Chicago.
Nowak, R.M. and J.L.Paradiso. 1983. Walker's Mammals of the World 4th Ed. Johns Hopkins University Press: Baltimore.


text:
I was thinking this morning about how we tend to think of one another in terms of categories: he's rich, she's poor; she's pretty, he's ugly; she's smart, he's dumb; he's black, she's white. We typecast people. That's how we know how to deal with them. We respond one way to someone if they're rich and another way to someone if they're poor. We approach different people according to the socio-economic, cultural, or racial category we've placed them in. Of course, this dehumanizes people as it fails to grasp that all of us are "fearfully and wonderfully made."
This is why I love the story of Jesus calming the storm in Matthew 8:27. After this miraculous event, his amazed disciples say to one another, "What manner of man is this that even the wind and sea obey him?" They had no category for Jesus. He was beyond typecasting. He transcends every barrier that separates us from one another—and he intends for his body, the church, to follow suit.


text:
ODT Online
ODT /directory
An appeal by Dotcom's lawyers, arguing for access to all the
FBI investigation files before his extradition hearing, was
dismissed by the Supreme Court at Wellington today.
But the Mega millionaire said he will not be defeated.
Following the decision Dotcom posted a message to his 354,000
followers on Twitter.
"Being defeated is often a temporary condition. Giving up is
what makes it permanent," he said.
The defiant message came shortly after a sad-faced emoticon,
tweeted immediately after the ruling was announced.
Dotcom, Finn Batato, Mathias Ortmann and Bram van der Kolk
are defending charges of mass copyright infringement, online
piracy, and money laundering.
Today's judgment comes after the Court of Appeal reversed a
decision by the District Court to allow Dotcom the documents.
The District Court decision had been upheld by the High
Court, however Dotcom's lawyers were granted leave to seek an
appeal in the Supreme Court after the Court of Appeal
reversed the decision.
"The District Court was wrong to order disclosure of the
documents concerned," Justice John McGrath said today.
"The appeal has been dismissed and costs have been reserved."
The District Court had "no power" to order the US to disclose
information, the Supreme Court ruled, saying no argument had
been put forward to "suggest that exceptional circumstances
exist that would warrant a formal request" for further
information from the US authorities.
"There is no indication of a lack of candour on the part of
the United States, or any other abuse of process, and the
appellants have not pointed to any reason why, without the
requested information, they will be unable to meaningfully
challenge the case against them," the ruling said.
The Supreme Court said Dotcom and his team would have greater
knowledge of how Megaupload worked than the FBI.
"Whereas the United States relies on expert analysis and
inference to identify the business structure and operations
of Megaupload, the appellants have first-hand knowledge of
Megaupload's purpose and business model.
"It cannot be said that the appellants will, without more
information, be deprived of the opportunity to properly
contest the case against them, as presented in the record of
the case."
The defence already had access to much of the information
gathered as evidence by the US, the court said.
That includes extracts from a large number of emails, data
from Megaupload servers, a network analysis of how the
websites operated and an analysis of all the financial
The proposed testimony of investigators who went undercover
as users of the Megaupload websites, and a number of experts
in copyright ownership, were also included in the evidence,
Justice McGrath said.
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text:
PLYMOUTH, Minn., April 12, 2013 /PRNewswire/ -- The Mosaic Company (NYSE: MOS) has been recognized on Corporate Responsibility Magazine's 14th annual 100 Best Corporate Citizens List, known as the world's top corporate responsibility ranking based on publicly available information. This is the fourth year in a row that Mosaic has been named to the list.
Mosaic ranks No. 52 on the list—in recognition of its conservation and environmental stewardship efforts, as well as its philanthropy and transparency. Notable accomplishments include:
- Mosaic has decreased its groundwater use at its Central Florida operations by 50 percent over the last 20 years.
- Mosaic produces approximately 6.9 million gigajoules of clean electricity through waste heat capture—to satisfy 47 percent of company-wide electrical demand.
- Mosaic is a signatory to the United Nations Global Compact, affirming its deep commitment to operate according to universally accepted principles in the areas of human rights, labor, environment and anticorruption.
Mosaic also was noted for its philanthropy and the investments of The Mosaic Company Foundation—including The Mosaic Villages Project—which is moving 300,000 smallholder farmers out of poverty in ten countries on three continents. Watch videos about how The Mosaic Villages Project farmers are improving their livelihoods by growing more food through sustainable intensification and agronomic practices.
"Without a doubt, Mosaic's greatest strength is its workforce. Our more than 8,000 global employees translate Mosaic's values of integrity, excellence, sustainability and connectivity into action every day," said Jim Prokopanko, President and CEO, The Mosaic Company. "It's because of their dedication to excellence that our company stands out—to customers, shareholders and the global business community. We're grateful for their achievements and this 100 Best Corporate Citizens List recognition."Mosaic's ranking is based on more than 298 data points in seven categories, including environment, climate change, human rights, philanthropy, employee relations, financial performance and governance. The 100 Best Corporate Citizens List's open and transparent methodology is governed by a Methodology Committee of the Corporate Responsibility Officers Association.


text:
The £112.6 million fund produced a total return of 29.9% last year on a net asset value basis versus 21% for the FTSE All-World Index.
The trust was helped by a strong position in the Japanese stock market which has long been a barren hunting ground for asset managers but had a good 2013.
Trust chairman Teddy Tulloch said: "Our heavy weighting in Japanese shares was a major factor in the investment performance.
"The Japanese equity market suffered in the aftermath of the tsunami in early 2011. However, our investment manager's conviction about the exceptional value available in many Japanese shares was finally rewarded in 2013."
Dr Nairn increased his weighting by cutting investment in the UK and the rest of Asia in early 2013.
Among his best performers was electronics company Panasonic which in 2012 was forced to take large impairments after being hit by the strong yen. In 2013 its share price rose 124% in local currency terms.
Dr Nairn said: "We consider that it is still not yet expensive."
The trust was also aided by relatively big positions in the US and continental European companies.
Mr Tulloch said: "In the second half of the year, we started to take some profits in Japan as a number of share prices had risen to levels that no longer represented good value.
"The money raised was reinvested in continental Europe where many shares still offered value."
By the year-end 30.9% of the fund was invested in continental European firms and 30.2% in Japan.
Among those holdings cut back or sold were retailer Seven and I and technology company Fujitsu.
Mr Tulloch said: "After such good gains in share prices in the developed markets, many shares are looking expensive and so are vulnerable to profit taking. However, we believe it is still possible to identify shares that represent good long-term value."


text:
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- Wrangler® Camry Short
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60LNW Wrangler® Lennon Short
Price: $19.99
Comfortable and loaded with pocket space, they're ready for any adventure.
Designed with two side cargo pockets with flap closure, one added pocket for extra storage, and a hidden cell phone pocket.
- Styling: Cargo
- Fit: Regular
- Length: Hits at knee
- Front Closure Type: Zipper fly with button closure
- Back Pocket Closure: Two back pockets with no closure
- Fabric: 100% Cotton
- Care: Machine Wash. For further care instructions, see label inside garment.
Wrangler® Lennon Short 5 5
These shorts are awesome! I've been wearing these shorts for a few years and have never been dissatisfied. They fit properly, wear well and are perfect in every way. I usually buy them from a large discount retailer but they do not have stock due to the winter season. My purchase directly from Wrangler.com couldn't have been any easier. November 19, 2013
Classy and Casual I'm only in my 30's but I try not to dress like a teenager during the summer months. I've been wearing Wrangler cargo shorts for several years now. They let me wear something casual and stylish and still appear mature. The shorts have nice clean lines; no bulky over-sized pockets, wrinkled pockets flaps, fading or fraying, or flaring legs holes. You can wear t-shirts with them and use it as a utility short or wear a nice polo or button down and dress them up. They are comfy, last forever, and don't wrinkle 5 seconds after you put them on. I like the new slightly larger hidden phone pocket that fits an iPhone. I've paid twice as much for shorts like this in the past. Thankfully I found these at Target years ago. August 20, 2013
Good Cargo Shorts I like Wrangler cargo pants and shorts because the cargo pockets have easy to use Velcro closures; you don't have to fiddle with snaps or buttons. These are well-made and comfortable in hot weather. July 12, 2013
Different colors, different sizes I bought a pair in black size 34. It fits great. I bought a pair in putty size 34 and it is too tight. July 12, 2013
The only shorts I like Awesome shorts. It's a shame the stores run out in mid-July. July 3, 2013
1-5 of 5
(2 Questions : 3 Answers)
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Has expert answer
Has staff answer
2 answers
What is the inseam length?
Age:Â Over 65
Gender:Â Male
1 year, 2 months ago
West Coast, almost
0out of 0found this question helpful.
Was this helpful?helpful0unhelpful0http://reviews.wrangler.com/answers/7135/product/60LNW/question/1782460/undohelpfulness.djs?format=embeddedhtml&sessionparams=__BVSESSIONPARAMS__&return=__RETURN__&innerreturn=http%3A%2F%2Freviews.wrangler.com%2Fanswers%2F7135%2Fproduct%2F60LNW%2Fquestions.djs%3Fformat%3Dembeddedhtml%26scrolltotop%3Dtrue%26expandquestion%3D1782460&user=__USERID__&authsourcetype=__AUTHTYPE__&submissionparams=__BVSUBMISSIONPARAMETERS__&submissionurl=__BVSUBMISSIONURL__
Answer:ÂThe inseam of this short is approximately 10"
1 year, 2 months ago
0out of 0found this answer helpful.
Was this helpful?helpful0unhelpful0http://reviews.wrangler.com/answers/7135/product/60LNW/answer/1696407/undohelpfulness.djs?format=embeddedhtml&sessionparams=__BVSESSIONPARAMS__&return=__RETURN__&innerreturn=http%3A%2F%2Freviews.wrangler.com%2Fanswers%2F7135%2Fproduct%2F60LNW%2Fquestions.djs%3Fformat%3Dembeddedhtml%26scrolltotop%3Dtrue%26expandquestion%3D1782460%26expandanswer%3D1696407&user=__USERID__&authsourcetype=__AUTHTYPE__&submissionparams=__BVSUBMISSIONPARAMETERS__&submissionurl=__BVSUBMISSIONURL__
Answer:Âabout 12" gos to the knee
Age:Â Over 65
Gender:Â Male
8 months ago
Washington State
0out of 0found this answer helpful.
Was this helpful?helpful0unhelpful0http://reviews.wrangler.com/answers/7135/product/60LNW/answer/1934928/undohelpfulness.djs?format=embeddedhtml&sessionparams=__BVSESSIONPARAMS__&return=__RETURN__&innerreturn=http%3A%2F%2Freviews.wrangler.com%2Fanswers%2F7135%2Fproduct%2F60LNW%2Fquestions.djs%3Fformat%3Dembeddedhtml%26scrolltotop%3Dtrue%26expandquestion%3D1782460%26expandanswer%3D1934928&user=__USERID__&authsourcetype=__AUTHTYPE__&submissionparams=__BVSUBMISSIONPARAMETERS__&submissionurl=__BVSUBMISSIONURL__
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1 answer
Lennon Cargo Shorts Availability?
when are you going to get more "Lennon Cargo Shorts" in stock? Specifically the khaki size 44.
Age:Â Over 65
Gender:Â Male
1 year, 5 months ago
0out of 0found this question helpful.
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Answer:ÂUnfortunately, we do not currently offer the Lennon Cargo short in a 44" waist in either dark putty or camel. However, we do have the military khaki version of the Lennon Cargo short in a 44" waist currently in stock. This is a darker khaki color than either the dark putty or the camel, but may be what you're looking for. If you have any further questions about this short, please don't hesitate to reach out to our consumer relations team at 888-784-8571 or through this page (https://www.wrangler.com/store/WRG_...) and a team member can assist you.
1 year, 5 months ago
0out of 0found this answer helpful.
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1 of 1
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text:
Ox and Bull Trading Co.
One-Piece Onyx and Polished Silver Cufflinks
- Color:
- black
Please select an available size.
Please select a size.
Share This Item: Email
Description: Plated base metal circular cufflinks
- Genuine onyx inlay
- Fixed backing
- Diameter: .625"
Material: Plated Base Metal, Onyx
Care: Spot clean
Brand: Ox and Bull Trading Co.
Origin: Imported


text:
STARmeterTop 5000
Down 56 this week
Dustin Clare
A rising Australian actor, Dustin Clare is originally a native of New South Wales, Australia. Dustin's road to acting began during his student years at the Western Australia Academy of Performing Arts. He graduated with a degree in Dramatic Arts in 2004. His first steps into acting began in 2003 with a film short, before graduating to more ... See full bio »
Born:in Australia
More at IMDbPro » Contact Info: View agent and company
Represent Dustin Clare? Add or change photos


text: Event: 10th Annual Corn Maze at Lollie Saturday, September 27, 2014 - Saturday, November 01, 2014 Central Region Get Directions Calculate Trip City: Mayflower Region: Central Building / Point of Interest: Schaefers Farm Address: 861 Lollie Road Mayflower, AR 72106 Contact: Pat Schaefers Phone: 501-269-7903 Email: firstname.lastname@example.com Website: www.cornmazeatlollie.com Event Details: Two mazes, a scenic 30-45 minutes hayride, concession food, port-a-potties. Corn Maze Express train ride, large corn box for play, hay tunnels, playground equipment, picnic tables, kiddie toy rides (wagons, trikes, cars etc.) hay mounds, sand play area, and decorated picture sites. Admission: Adults $7, 60+ $6, kids 3-12 $5, 2 and under free, also special group rates Accessible


text:
Another coffee time debate.
I wanted to take a group to a zoo nearby but some people complained that it would be endorsing cruelty to animals .
What do you think? Personally I think if they save species, promote kindness and allow people to see different animals then they're a good thing but I can see the contrary view


text:
Club Drugs Center - Kokomo, IN
WebMD Physician Directory
Kokomo, Indiana
Club Drugs
How Are Club Drugs Abused?
Other Related Club Drugs Articles
Emergency Contact for Kokomo
Nearby Kokomo Hospitals *
St Joseph Hospital
Howard Regional West Campus
Community Howard Regional Hlth
IU Health Tipton Hospital
Dukes Memorial Hospital
St Vincent Mercy Hospital
Logansport State Hospital
Four-County Counseling Center
Logansport Memorial Hospital
Grant-Blackford Mental Health
Marion General Hospital
St Vincent Frankfort Hospital
Veteran Affairs Medical Center
Wabash County Hospital
Riverview Hospital
Witham Memorial Hospital
Community Hospital Of Anderson
IU Health White Memorial Hosp
St Vincent Carmel Hospital
*Provider Directory Terms of Use:
The WebMD 'Provider Directory' is provided by WebMD for use by the general public as a quick reference of information about Providers. The Provider Directory is not intended as a tool for verifying the credentials, qualifications, or abilities of any Provider contained therein. Inclusion in the Provider Directory does not imply recommendation or endorsement nor does omission in the Provider Directory imply WebMD disapproval.


text:
Weavers Way Co-op is a member-owned, cooperative grocery store with locations in the West Mt. Airy and Chestnut Hill neighborhoods of Philadelphia. We offer a friendly shopping environment and reasonably priced, quality products that are local, sustainable, organic, fairly traded and healthful.
Co-op News
Join your fellow Co-op owners for business and pleasure and to bid farewell to GM Glenn Bergman at the Weavers Way Spring General Membership Meeting at Morris Arboretum.
Petapalooza, Weavers Way's fifth annual festival and photo contest celebrating dogs, cats, chickens, pocket pets and the people who love them, takes place Saturday, June 6, on Carpenter Lane outside the Mt. Airy store.
With a little help from the homesteading pros, this can be the summer you turn both of your thumbs green! From volunteer days where you can learn techniques firsthand from the Weavers Way farmers, to workshops that will remake your kitchen into a DIY hub, Weavers Way will have you sprouting into a master of the domestic arts this season.
Join us Saturday, June 13, for our second annual Hoedown! It's an evening of locally sourced, farm-fresh food, live music and outdoor games. Kick off the summer amid the 2-acre Weavers Way Farm on the grounds of Awbury Arboretum.


text:
Best Power Play New York 2005 - SHELDON SILVER
Years of work by hundreds of people vanished in an instant when State Assembly Speaker and LES legislator SHELDON SILVER killed off the Jets stadium and the NYC 2012 Olympic bid with a non-wave of his hand. Isn't it just terrible how Albany concentrates so much power in the hands of so few people?
Best Bill Designed to Have Women Simultaneously Drop Their Pants >> << Best Unnecessary but Totally Rad Use of the City's Money


text:
Everyone in the Congress, from ordinary workers to party general secretaries, is baying for party vice-president Rahul Gandhi's blood.
They want Sonia Gandhi to take charge and stop "radical reforms" initiated by her son Rahul, who they believe is the main culprit behind the party's debacle. But nobody, it seems, has the guts to take a stand against him publicly. So, the ultimate catchword is: Congress cannot let go dynastic rule.
A spate of resignations is expected at the Congress Working Committee (CWC), the party's highest decision making body, meeting scheduled on Monday. Both mother and son might offer to step down, taking moral responsibility for the unprecedented defeat. But that will be rejected by the CWC in one voice. And to complete this drama, some 40 members of the extended CWC has sent in their resignation letters, hoping they will be asked to continue.
"We understand there was an anti-incumbency factor... people were fed up with seeing our faces daily. But we had never imagined a defeat of such magnitude," a party functionary told dna.
While veteran leaders are annoyed with the way the campaign was put together, younger leaders feel the way the UPA functioned was largely to blame. Another worrying factor is the drastic drop in its vote share. In 1977, the party polled 34.50% votes. In 1999, the party got only 114 seats; but it still had 28.30% votes.
This elections, Congress' voting share has plummeted to 20%.
The younger generation believes the government should have taken the Anna Hazare-Arvind Kejriwal movement seriously. About the various scams, they feel the government should have brought culprits to the book. These would have gone a long way in rebuilding peoples faith in the Congress.
Seniors say the buck stops at Rahul Gandhi. For, he was not only a reluctant leader but he also had deep disdain for seniors and everything that had been put in place over the years. "Instead of reviving social contacts and energising workers at the ground level, Rahul focused too much on systemic reforms within the party headquarters," a leader at the AICC headquarters said.
But his idea had hardly any takers. Party workers believed it was rhetoric and a non-practical idea in the Indian context. Had he focused on people welfare, anti-poverty measures and price rise, it would have been much better.
"Everybody saw the fate of primaries. In the two Delhi seats where he forced primaries — Delhi north-east and New Delhi — both candidates JP Agarwal and Ajay Makan came third in the elections and lost by over 3 lakh votes," the leader said. Instead of banking on experienced advisors and hardcore politicians, Rahul relied heavily on classy foreign-educated boys and girls who sat in air-conditioned rooms and analysed ground realities.
Also, a major blunder was Sonia Gandhi withdrawing herself form the scene at a crucial time and initially refusing to accept Modi as a challenge. Some even gloated that he would consolidate secular votes in favour of the Congress. Poor communication management was another issue.
Rahul's close aides thought the more Modi communalised the environment, the better the chances of Muslims and others supporting the Congress.
When internal surveys predicted a rout, messages went to the government to fix Modi in the Snoopgate case. But Manmohan Singh shot it down.
The knives are out for CMs of Congress-led states as they did not help fund the party coffers. "A chief minister of a southern state was asked to contribute some Rs450 crore for election campaign. But he sent a paltry Rs27 crore," a leader said.
And then there was the election management committee, which didn't meet at all. Everything, from coining party slogans to drafting political messages, was left to the war room boys. Master strategist Jairam Ramesh picked up an advertising agency, whose slogans and video clips just could not stand up to the BJP onslaught. Ramesh, along with Sam Pitroda and Deep Kaul, son of former diplomat TN Kaul, called the shots in Rahul's war room, instead of handing things over to political veterans.


text:
A lot has been published and broadcast about Internet users' "right to be forgotten," long before and since last week's ruling by a high court in Europe (see the BBC), including the important points that… Scratching the surface. Suing a search engine to take down links to offending content doesn't mean the content itself gets […]
Tag Archives | Right to Be Forgotten
Remember: The 'right to be forgotten' is shared
By Anne Collier on May 21, 2014 in Commentaries - Staff, Featured, NetFamilyNews
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