text:
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BMB reports
*Corresponding author. Hee Gu Lee, Ph.D. or Jong-Wan Kim, M.D.,
Ph.D. Tel: 82-42-860-4182, 82-41-550-6662; Fax: 82-42-860-4593,
82-41-555-7155; E-mail: email@example.com or email@example.com
#These authors contributed equally to this work.
Received 4 July 2008, Accepted 7 July 2008
Keywords: Akt, Mac-2 BP , NGF, PI3K
Induction of Mac-2BP by nerve growth factor is regulated by the
PI3K/Akt/NF-κB-dependent pathway in the HEK293 cell line
Yuk Pheel Park1, Seung-Chul Choi1, Bo-Yeon Kim1, Jong-Tae Kim1, Eun Young Song1, Seong Ho Kang2, Do-Young Yoon3,
Sang-Gi Paik4, Kwang Dong Kim5, Jong Wan Kim6,*,# & Hee Gu Lee1,*,#
1Stem Cell Research Center, KRIBB, Daejeon, 2Department of Chemistry and Basic Science Research Institute, Chonbuk National
University, Jeonju, 3Department of Bioscience and Biotechnology, Konkuk University, Seoul, 4Department of Biology, Chungnam National
University, Daejeon, 5Division of Applied Life Science, Gyeongsang National University, Jinju, 6Department of Laboratory Medicine,
Dankook University College of Medicine, Cheonan, Korea
Mac-2BP is a ligand of the galectin family that has been suggested
to affect tumor proliferation and metastasis formation. We as-
sessed Mac-2BP expression at the transcriptional and transla-
tional levels to evaluate nerve growth factor (NGF)-induced
Mac-2BP expression. A time kinetic analysis using reverse tran-
scription-polymerase chain reaction showed that NGF-induced
Mac-2BP transcript levels were 4-5 times higher than in controls.
Mac-2BP enzyme-linked immunosorbent assay and immuno-
fluorescence staining showed a 2-3-fold increase in intracellular
and secreted Mac-2BP as a result of NGF stimulation. This in-
crease was regulated by Akt activation and NF-κ κB binding. p65
and p50-NF-κ κB are major transcriptional factors in the Mac-2BP
promoter region, and were shown to be regulated in accordance
with the Akt activation states. Collectively, these results suggest
that NGF induces Mac-2BP expression via the PI3K/Akt/NF-κ κB
pathway. [BMB reports 2008; 41(11): 784-789]
Mac-2BP is a large oligomeric glycoprotein that has been identi-
fied in the fluid from human breast cancer cells (1,2). Supranor-
mal serum levels of Mac-2BP have been found in patients with
breast, lung, pancreatic, and gastric tumors, Non-Hodgkin's
lymphoma, asthma, and human immunodeficiency virus in-
fection (2-9). The reported evidence implicates Mac-2BP as a di-
agnostic and/or prognostic marker of poor outcome in several
tumor types. Mac-2BP, like other members of its family, is in-
volved in the host response to tumors and infections (10).
Moreover, several reports have shown that the protein may
function in cell adhesion processes. In particular, it has been
demonstrated that Mac-2BP, as a ligand of galectin-1, -3, and -7,
may promote homotypic cell-to-cell contacts (11,12), or may
regulate cell adhesion by binding to cellular matrix proteins in-
cluding β1-integrin, collagens, and fibronectins (13). Therefore,
Mac-2BP may favor the establishment of new tumor colonies
via enhancement of adhesive interactions between tumor cells
and the extracellular matrix (14,15).
Neurotrophins are a family of structurally related secretory
proteins that are involved in the survival, development, and
death of specific populations of neuronal and non-neuronal
cells (16). Nerve growth factor (NGF) is the best-characterized
member of this family. NGF interacts with two classes of mem-
brane receptors: the TrkA proto-oncogene product p140TrkA,
which exhibits intrinsic tyrosine kinase activity, and a secon-
dary receptor, p75NTR, which is a member of the tumor ne-
crosis factor (TNF) receptor family (17).
In the current study, we have determined that NGF induces
Mac-2BP expression at the transcriptional level, and is depend-
ent on an Akt/NF-κB-dependent pathway.
Nerve growth factor induces Mac-2BP expression
To evaluate the role of NGF in Mac-2BP expression, we uti-
lized reverse transcription-polymerase chain reaction (RT-PCR).
NGF was applied to serum-starved HEK293 cells; Mac-2BP
transcripts peaked within 6 h and then slowly diminished over
time. Mac-2BP transcripts were increased steadily as the result
of NGF treatment (Fig. 1A). Mac-2BP ELISA showed a 2-3-fold
increase in the secreted Mac-2BP in culture supernatants of
HEK293 or SNU-638 cell line treated with NGF (Fig. 1B). An
increase of Mac-2BP was verified via immunofluorescence
staining with an anti-Mac-2BP monoclonal antibody. The in-
crease in Mac-2BP was verified by immunofluorescence stain-
ing with an anti-Mac-2BP monoclonal antibody. We inhibited
glycosylation by pretreatment with 10 μg/ml tunicamycin for
30 min prior to NGF treatment, and staining of Mac-2BP. As
Mac-2BP secretion was prohibited by the inhibition of glyco-
sylation, intracellular Mac-2BP was more readily detectable in
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Yuk Pheel Park, et al.
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Fig. 1. Mac-2BP expression induced by NGF. (A) After the cells
were incubated with NGF for 6-24 h, cell morphology was assessed
under a microscope (left panel). Total RNAs were prepared from the
stimulated cells, and RT-PCR was conducted with Mac-2BP or
GAPDH primers. (B) The cells treated for 24 h with NGF were
lysed, and Mac-2BP ELISA was conducted with the protein extracts
that had been prepared. (C) HEK293 cells were serum-starved for
18 h followed by 24 h of NGF and/or tunicamycin treatment. For
immunofluorescence analysis, the cells were fixed and stained with
Mac-2BP monoclonal antibody and cellular localization and ex-
pression of Mac-2BP were observed with a confocal microscope.
Fig. 2. Regulation of Mac-2BP expression by NGF at the transcrip-
tional level. (A) Cells were pre-treated with 2 μg/ml of CHX for 40
min prior to NGF treatment. After an additional 24 h of incubation,
the cells were lysed and Mac-2BP ELISA was conducted using cyto-
solic soluble proteins. (B) The -2377 bp of the human Mac-2BP pro-
moter region was constructed and transfected into HEK293 cells using
Lipofectamine reagent, and then subjected to NGF treatment for 24
h. The cells were lysed, and the relative luciferase activities expressed
as the ratio of the pGL3 reporter activity to that of the pRL control
plasmid. Values are expressed as means ± SD of three independent
measurements. (C) Nuclear extracts were prepared from the HEK293
cells stimulated with 50 ng/ml of NGF for 24 hr, and the DNA binding
activity of NF-κB was determined with a consensus dsDNA oligomer
probe via an EMSA assay. The arrow indicates NF-κB complex.
these cells than in the NGF-treated cells (Fig. 1C).
NGF-induced Mac-2BP expression is regulated at the
transcriptional level
To determine whether the increase in Mac-2BP was derived from
novel protein synthesis, we induced the inhibition of protein syn-
thesis by a 40 min pre-incubation with cycloheximide prior to the
NGF treatment. NGF-induced upregulation of Mac-2BP was de-
creased to the levels seen in the untreated control cells (Fig. 2A).
Moreover, in an effort to assess the regulation steps of Mac-2BP
expression, we cloned -2377 bp of the Mac-2BP promoter flank-
ing regions within the pGL3 basic luciferase reporter vector sys-
tem (pGL3-Mac Pro-2377), and transiently introduced it into
HEK293 cells. The relative luciferase activities of the Mac-2BP
promoter were clearly increased by NGF (Fig. 2B)
To test whether NF-κB might be involved in the expression
of Mac-2BP, serum-starved HEK293 was treated for 24 h with
NGF, and the nuclear extracts were prepared. The DNA bind-
ing activity of NF-κB was clearly enhanced to a greater degree
than was observed with the control extracts. This indicates that
NGF increases the expression of Mac-2BP at the transcriptional
level via NF-κB activation (Fig. 2C).
NGF-induced Mac-2BP expression is dependent on the
PI3K/Akt/NF-κ κB pathway
To evaluate the signal transduction of Mac-2BP expression by
NGF, we assessed the activation of MAP kinase or Akt. As
shown in Fig. 3A, phosphorylated-p140TrkA tyrosine kinase re-
ceptor was detected within 1 h, and the results of time kinetic
analysis showed that phospho-p44/42 MAP kinase (Thr202/
Tyr204) and phospho-Akt (Ser 473) were detected between 3 h
and 6 h of incubation with NGF (Fig. 3B). To evaluate the rela-
tionships between Akt, NF-κB, and Mac-2BP expression, we
pre-incubated HEK293 cells with PI3 kinase inhibitors prior to
NGF treatment. Mac-2BP expression was analyzed at the tran-
scriptional and translational levels using RT-PCR and Mac-2BP
enzyme-linked immunosorbent assay (ELISA) (Figs. 3C-E) in-
dicating that the inhibition of PI3 kinase induced a reduction
in the Mac-2BP transcripts and its protein via the inhibition of
Akt, although this did not completely suppress the expression.
These results indicate that the expression of Mac-2BP as a con-
sequence of NGF signaling is dependent on Akt activation.
DNA binding activity of NF-κ κB is crucial for Mac-2BP
There are two putative binding sites of NF-κB on the promoter
region of Mac-2BP (NCBI number; U91729). To characterize
the Mac-2BP promoter region, we conducted a competition as-
say using cold probes and a supershift analysis with anti-p50 or
p65 antibodies. The results of the competition assay showed
that the NGF-treated cells exhibited profound binding of the
consensus or upstream and downstream NF-κB probes as com-
pared to the untreated control cells. A downstream NF-κB site
also evidenced stronger binding than the upstream NF-κB site
(Fig. 4A).
To investigate the effects of PI3K inhibitors on NF-κB activa-
tion, we evaluated the localization of the NF-κB subunits. As
shown in Fig. 4B, p65 and the phosphorylated form of p65
were polarized in the nuclear region. The NGF signal also in-
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Regulation of Mac-2BP expression
Yuk Pheel Park, et al.
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Fig. 3. Critical role of NGF signaling via a PI3K/Akt-dependent path-
way in Mac-2BP expression. (A) After NGF treatment for the indicated
amounts of time, the HEK293 cells were lysed and soluble cytosolic
proteins were immunoblotted with phospho-p140TrkA antibody. (B)
HEK293 cells were harvested at the indicated time points and the cy-
tosolic extracts were prepared. Total proteins of p42/44 MAP kinase,
Akt, or phosphorylated forms of these proteins were measured using
Western blot analysis. (C) Starved HEK293 cells were pre-treated with
PI3K inhibitors for 30 min prior to NGF treatment. RT-PCR was con-
ducted with Mac-2BP-specific primer. The relative values of the PCR
products were calculated using a densitometer (MAC-2BP/GAPDH).
(D) PI3K inhibited cells were lysed and the total Akt or phosphory-
lated Akt was determined using Western blotting. (E) Mac-2BP ex-
pression was assessed by Mac-2BP ELISA.
Fig. 4. Critical effect of NF-κB binding on Mac-2BP expression. (A)
EMSAs were conducted in order to assess NF-κB binding activity us-
ing commercial consensus or upstream (u) and downstream (d) puta-
tive NF-κB sequences of the Mac-2BP promoter. Competition assays
were conducted with a molar ratio of 20X or 40X cold probes. (B)
Starved HEK293 cells were pre-treated with PI3K inhibitors for 30
min, and were incubated with NGF for 24 h. The cells were pre-
pared as nuclear and cytosolic proteins. Western blotting was con-
ducted with the indicated antibodies.
creased the activation of the p65 NF-κB subunit, whereas it
was suppressed by LY294,002. Wortmannin increased phos-
phorylated p65 or p50 levels in the nuclear regions of the cells
indicating that wortmannin may regulate NF-κB activation oc-
curring via other pathways. Taken together, the results indicate
that NGF may increase Mac-2BP expression by the induction
of NF-κB binding on its promoter region, and is also depend-
ent on the PI3K/Akt/NF-κB pathway.
We have recently determined that Mac-2BP may prove useful
as a diagnostic and prognostic marker for gastric cancers (8). In
the current study, to evaluate the molecular basis of Mac-2BP
expression in HEK293, we assessed the effects of NGF on
Mac-2BP expression and dissected the signal pathway involv-
ing the binding of NGF to its receptor.
As shown in Fig. 1, NGF induced Mac-2BP expression in hu-
man embryonic kidney 293 cells. The Mac-2BP transcript level
achieved a peak at 6 h and then decreased slightly until 24 h.
NGF-induced Mac-2BP was also detected in a quantitative
Mac-2BP ELISA assay (Fig. 1B). NGF was shown to play roles in
the induction and secretion of Mac-2BP, and immunofluorescence
staining with Mac-2BP monoclonal antibody evidenced the up-
regulation of Mac-2BP as a result of NGF treatment. Because
Mac-2BP is known to be a highly glycosylated protein, we pre-
treated samples with 10 μg/ml tunicamycin for the easy detection
of Mac-2BP. Therefore, the sample containing NGF plus tunica-
mycin allowed for easier observation of Mac-2BP as compared to
the negative or NGF-treated samples (Fig. 1C).
We also demonstrated that the induction of Mac-2BP result-
ing from NGF treatment is regulated at the transcriptional level,
as shown in Fig. 2. Experiments in protein synthesis inhibition
and reporter assays demonstrated the regulation of Mac-2BP
expression upon transcription by NGF, and the DNA binding
activity of NF-κB was shown to be involved in the transcription
of Mac-2BP. To evaluate the signal cascades of Mac-2BP ex-
pression, we analyzed the activated states of the p140TrkA re-
ceptor, p42/44 MAP kinase, and Akt (Fig. 3). The NGF signal
induced an increase in the phosphorylated form of p140TrkA
within 1 h of NGF stimulation, and the activation of p42/44
MAP kinase and Akt were also increased as a consequence of
NGF treatment. This result also suggests that Mac-2BP is sub-
ject to control by NGF as a result of PI3K/Akt/NF-κB signaling.
In neuronal systems, the binding of NGF to p140TrkA or p75NTR
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Regulation of Mac-2BP expression
Yuk Pheel Park, et al.
BMB reports
activates several signaling pathways, including the phosphoi-
nositide-3-kinase (PI3K) and mitogen-activated protein kinase
pathways. This also results in the activation of several transcrip-
tional factors (18, 19) including NF-κB (20). NGF activates
NF-κB and AP-1 in sympathetic neurons, and NGF-inducible
NF-κB is required for neuronal survival (21).
We report here that two NF-κB binding sites on the Mac-2BP
promoter region are critical DNA-bound components for the
expression of Mac-2BP, and that the activity is regulated by the
PI3K/Akt pathway. The Mac-2BP transcripts or its secreted
products were regulated via the inhibition of PI3K inhibitors in-
cluding wortmannin and LY294,002, and its regulation was po-
tentially involved in NF-κB transactivation (Figs. 2C, 3C-E). The
identified sequence begins at the upstream position -1622/
-1611 and the downstream position -1580/-1589. NF-κB p50
usually binds to both positions containing the consensus se-
quence and promoter binding sites; however, p65-NF-κB dis-
played unique binding on the upstream site. Collectively, these
results suggest that p65-NF-κB binding is critical for Mac-2BP
expression, because p50-NF-κB lacks a transactivation domain.
The regulation of NF-κB activity by NGF and by Akt remains
a matter of some controversy, and the discrepancies among pre-
vious studies may be related in part to cell type specificity.
NGF-induced dissociation of the cytosolic p65/IκBα complex
via phosphorylation of IκBα at a tyrosine residue 42 has been
described (22). This mechanism results in nuclear translocation
of p65-NF-κB without significant degradation of IκBα. In addi-
tion, other cytokine signaling studies have shown that the tran-
scriptional activity of NF-κB is regulated independently of IκB.
For example, inhibition of IL-1 stimulated PI3K activity by pre-
treatment with LY294,002 or wortmannin also causes a dramatic
loss of NF-κB-dependent gene expression (23). Despite the dra-
matic decrease in NF-κB-induced gene expression, LY294,002
and wortmannin have no effects on interleukin-1 (IL-1) -stimulated
degradation of IκBα or the nuclear translocation or DNA bind-
ing of NF-κB itself in HepG2 cells (23).
Consistent with the evidence provided in the aforementioned
studies, our results also indicate that Mac-2BP is indeed regu-
lated via Akt expression or activation, and that NF-κB trans-
activation potential is crucial to the PI3/Akt-dependent pathway.
However, the nuclear localization of p65 is not inconsistent with
our prediction that NF-κB p65 and p50 proteins are localized at
the nuclear region even in unstimulated cells. In addition, wort-
mannin-pretreated cells, unlike LY294,002, evidenced higher
levels of nuclear phospho-p65-NF-κB than observed in the
NGF-treated cells (Fig. 4B). At this time, we do not have a clear
explanation for the differences in our results. However, these
findings reminded us that wortmannin could regulate NF-κB acti-
vation or transactivation occurring via multiple pathways. Our
results are similar to other reports that inhibitors of phophati-
dylcholine-specific phospholipase C and protein kinase C block
IL-1 and TNF-α-induced, NF-κB-dependent gene expression
without affecting cytokine-induced IκB degradation or the nu-
clear translocation or DNA binding of NF-κB (23,24).
Although the precise mechanism by which the regulation of
NF-κB by Mac-2BP occurs remains to be determined, the re-
sults of our study help to elucidate the manner in which NGF
and other signaling molecules regulate Mac-2BP. In summary,
we have uncovered new evidence indicating that Mac-2BP is
regulated by NGF at the transcriptional level, and that this oc-
curs in a PI3/Akt/NF-κB-dependent fashion.
Cell culture and treatment
HEK293 cells were cultured in MEM-alpha (Gibco-BRL, Grand
Island, NY) with 10% heat-inactivated fetal bovine serum (FBS;
Hyclone, Road Logan, Utah) and antibiotics in a humidified 5%
CO2 incubator at 37oC. Signaling blockers were administered
including 100 nM wortmannin and 10 μM LY294, 002 (Sigma-
Aldrich, St, Louis, MO) for 30 min, followed by stimulation with
50 ng/ml of recombinant human β-NGF (R&D Systems,
Minneapolis, MN) for the indicated amounts of time.
Anti-Mac-2BP was purchased from Alexis (San Diego, CA); an-
ti-p50 and total p42/p44 MAP kinase were obtained from
Upstate (Charlottesville, VA); anti-total Akt and phospho-Akt
(Ser 473), phospho-p42/p44 MAP kinase (Thr202/Tyr204), and
total p65 and phopho-p65 were purchased from Cell Signaling
Technologies (Danvers, MA); anti-histone H3 was obtained
from Santa Cruz Biotechnology (Santa Cruz, CA); anti-heat
shock protein 70 was obtained from Stressgen Biotechnologies
(Ann Arbor, MI).
Total RNA of the cells was isolated using TRI Reagent
(Molecular Research Center, Cincinnati, OH) using the manu-
facturer's instructions. The first-strand cDNAs were synthe-
sized with a ProSTART First-Strand RT-PCR kit ST (Stratagene,
La Jolla, CA). The following primer pair for PCR was used:
Mac-2BP sense; 5'-ACACGGTCATCCTGACTGC-3', Mac-2BP
antisense; 5'-ACAGGGACAGGTTGAACTGC-3'.
Mac-2BP ELISA
The cells were suspended in a cell lysis buffer (Cell Signaling
Technology, Beverly, MA) containing 1 mM PMSF and in-
cubated for 40 min on ice. The quantities of Mac-2BP in the cyto-
sol of the cells were measured via ELISA using an s90k/Mac-2BP
ELISA kit (Bender Med Systems GmbH, Vienna, Austria), in ac-
cordance with the instructions provided by the manufacturer.
Western blot analysis
Proteins were separated by 12-15% sodium dodecyl sulfate-
polyacrylamide gel electrophoresis (SDS-PAGE) and were trans-
ferred to Hybond-P PVDF membranes (Amersham Biosciences,
Buckinghamshire, UK). The nuclear and cytosolic fractions were
separated using a commercially available kit according to the
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Regulation of Mac-2BP expression
Yuk Pheel Park, et al.
BMB reports
protocol of the manufacturer (Pierce Biotechnology, Rockford,
IL). The membranes were blocked with 5% non-fat dry milk in
TBST (50 mM Tris-HCl, pH7.6, 150 mM NaCl, 0.1% Tween-20),
and were incubated for 2 h with the appropriate primary and
horseradish peroxidase (HRP)-conjugated secondary antibodies
at room temperature. After extensive washing, the protein
bands were visualized with an ImmobilonTM Western Chemilu-
miscent HRP substrate (Millipore, Billerica, MA).
Immunofluorescence confocal microscopy
Two million HEK293 cells were grown on 3-glycopropyl trime-
thoxysilane (Sigma-Aldrich) coated coverslips under starvation
conditions for 18 h. NGF (50 ng/ml) and NGF plus tunicamycin
(10 μg/ml) were administered for 24 h. The cells were then
washed with PBS, fixed, and permeabilized in BD Cytofix
/CytopermTM (BD Biosciences Pharmingen, San Diego, CA) for
20 min. After washing, the cells were blocked for 10 min in 1%
bovine serum albumin (BSA)/PBS at room temperature, washed,
and stained overnight with an anti-Mac-2BP monoclonal anti-
body (Alexis, San Diego, CA) at 4oC, followed by incubation
with fluorescein isothiocyanate (FITC)-conjugated anti- mouse
IgG and 4',6-diamidino-2-phenylindole (DAPI; Calbiochem,
San Diego, CA) staining. Cell-containing coverslips were
mounted onto glass slides using Dakocytomation mounting me-
dium (Produktionsvej 42, Glostrup, Denmark) and visualized
with a LSM510META Zeiss confocal microscope (Carl Zeiss,
Jena, Germany) at a magnification of 40X. The confocal images
were captured by the Zeiss LSM Image Browser program.
Luciferase reporter assay
The -2377 bp DNA fragment (nucleotide positions -2377 to
+61) including the 5' flanking region was inserted into the
pGL3-basic luciferase vector (Promega, Madison, WI). The in-
serted sequences were verified via DNA sequence analysis
(Genotech, Daejeon, Korea). For transient transfection, the
cells were plated at a density of 5 × 105 cells /12 wells on day
1. One day later, 0.5 μg of pGL3-Mac pro-2377 vector and 0.2
μg of Renillar vector as an internal control were co-transfected
with Lipofectamine PlusTM Reagent (Invitrogen). After 3 h of in-
cubation, the transfection media was changed with 1% fetal
bovine serum (FBS)/MEM-α, and the next day, NGF was ad-
ministered for an additional 24 h. The cells were lysed and a
luciferase assay was conducted using the Dual-LuciferaseR re-
porter assay system, in accordance with the instructions pro-
vided by the manufacturer (Promega). Luciferase activity was
determined using a Microlumat Plus luminometer (Berthold
Technologies, Bad Wildbad, Germany) by measuring light
emission for 10 s.
Preparation of nuclear extracts and electro mobility shift assay
Nuclear extract (5-7 μg) of nuclear extracts were incubated
with 32P-labeled probe in binding buffer (100 mM KCl, 30 mM
HEPES, 1.5 mM MgCl2, 0.3 mM EDTA, 10% glycerol, 1 mM
DTT, 1 mM PMSF, and protease inhibitor cocktail) in the pres-
ence or absence of competitor or antibodies for 20 min at room
temperature. The mixtures were then run on 6% polyacrylamide
gels in 0.5 ×TBE, and were dried and analyzed via autoradio-
graphy. The following probes that had been constructed through
the annealing of single-stranded oligonucleotides were utilized
for the EMSAs: upstream NF-κB site (NF-κBu -1622/-1611)
sense '-GGCCTCTGGGTTTTCCATTT-3', antisense, 5'-GGAGA
CCCAAAAGGTAAAAT-3'; downstream NF-κB site (NF-κBd-
1580/-1589) sense, 5'-GGATGGCTTAGGACTTTCCC-3', anti-
sense, 5'-TACCGAATCCTGAAAGGGCC-3'. The NF-κB con-
sensus oligonucleotides were purchased from Promega.
This work was supported by FG08-12-01 of the 21C Frontier
Functional Human Genome Project from the Ministry of
Science & Technology in the Republic of Korea, and by the
KRIBB Research Initiative Program.
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16. Lewin, G.R. and Barde, Y.A. (1996) Physiology of the
neurotrophins. Annu. Rev. Neurosci. 19, 289-317.
17. Friedman, W.J. and Greene, L.A. (1999) Neurotrophin sig-
naling via Trks and p75. Exp. Cell Res. 253, 131-142.
18. Ginty, D.D., Bonni, A. and Greenberg, M.E. (1994) Nerve
growth factor activates a Ras-dependent protein kinase that
stimulates c-fos transcription via phosphorylation of CREB.
Cell 77, 713-725.
19. Riccio, A., Pierchala, B.A., Ciarallo, C.L. and Ginty, D.D.
(1997) An NGF-TrkA-mediated retrograde signal to tran-
scription factor CREB in sympathetic neurons. Science 277,
20. Carter, B.D, Kaltschmidt, C., Kaltschmidt, B., Offenhauser,
N., Bohm-Matthaei, R., Baeuerle, P.A. and Barde, Y.A.
(1996) Selective activation of NF-kappa B by nerve growth
factor through the neurotrophin receptor p75. Science
272, 542-545.
21. Maggirwar, S.B., Sarmiere, P.D., Dewhurst, S. and
Freeman, R.S. (1998) Nerve growth factor-dependent acti-
vation of NF-kappaB contributes to survival of sympathetic
neurons. J. Neurosci. 18, 10356-10365.
22. Bui, N.T., Livolsi, A., Peyron, J.F. and Prehn, J.H. (2001)
Activation of nuclear factor-kB and bcl-x survival gene ex-
pression by nerve growth factor requires tyrosine phos-
phorylation of IkBa. J. Cell Biol. 152, 753-764.
23. Sizemore, N., Leung, S. and Stark, G.R. (1999) Activation of
phophatidylinositol 3-kinase in response to interleukin-1
leads to phophorylation and activation of the NF-κB p65/
RelA subunit. Mol. Cell Biol. 19, 4798-4805.
24. Bergmann, M., Hart, L., Lindsay, M., Barnes, P.J. and Newton,
R. (1998) IκBα degradation and nuclear factor-B DNA bind-
ing are insufficient for interleukin-1β and tumor necrosis fac-
tor-α-induced B-dependent transcription. J. Biol. Chem.
273, 6607-6610.


text:
The Comedy In Fifty Shades of Grey
By Amy Nicholson
Wild Tales Lays Bare Everyone's Awfulness
By Stephanie Zacharek
Approaching the Elephant Is a Bracing Education Doc
By Calum Marsh
By Stephanie Zacharek
By Inkoo Kang
The Results of Our 2014 Film Critics' Poll
By Voice Film Critics
By Alan Scherstuhl
The Blue Room (La Chambre bleue)
By Alan Scherstuhl
In time-honored concert-film structure, the backstage serves not just as backstory, but a purported inside track to the performers' just-like-us humanitya convention cemented as early as D.A. Pennebaker's Don't Look Back. This careful conveyance of realness continues in Block Party, now informed by the gimmicky logic of talk shows and reality TV. The film opens in Dayton, three days before the concert, where erstwhile resident Chappelle distributes golden block-party tickets to surprised Ohioans: the clerk at his grocery store, a local barber, and various folks on the city's downtown sidewalks. The mostly black Central State University Marching Band erupts in cheers when granted their invite to New York; a middle-aged white lady wonders if she should buy a thong for the event. The stunt is as old as Ed McMahon, but Chappelle's conversational ease makes his stint as "wee Willy Wonka" pleasantly unhokey. It also showcases his talents as unifier: After all, he's just about the only comedian who could yell "pussy hole" in publicas he does just to show one Daytonite that it's OK to swear in a movieand still garner Midwestern grandmas as fans. The rural locale isn't let off the hook as mere idyllic counterpoint to gritty New York. Two clean-cut lads tell how one got tossed the N-word on the golf course from an angry white male; astonished, they do nothing, not wanting anything to interfere with their getting on the bus to Bed-Stuy for Dave's party.
The event itselfevidently planned from concept to execution as much for Ellen Kuras's artful camerawork as the neighborhood folkstakes place on an unassuming stretch of Brooklyn street that includes a day care center once attended by Biggie Smalls. The show provides a locals-heavy lineup handpicked by Chappelle (many alums of his TV show), among them Mos Def, Kanye West, Talib Kweli, Dead Prez, and a reunited Fugees. Erykah Badu appears in a vast Afro cloud that would put Angela Davis to shame. Surprises include not just Big Daddy Kane but Black Panther legacy Fred Hampton Jr., who entreats the crowd to raise their hands in the air for more than just the music. So while the mostly black and Latino Brooklyn audience may be demographically pre-planned, it is also an act of momentary utopia; as Ahmir "?uestlove" Thompson of the Roots remarks to Chappelle backstage, both performers share the frequent experience of playing to audiences that don't look like them. Unfiltered observations like these give a critical edge to what otherwise would simply be a well-crafted concert doc shot during one gently sundowning autumn day.
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subset: commoncrawl


text:
Privacy and Cookies
Shannon airport has appointed Patrick Edmond as its group strategy director and managing director of its International Aviation Services Centre.
Edmond, who founded aviation consultancy e2consult, will be responsible for developing the cluster of aviation-related businesses around the Irish airport. He will also direct Shannon's overall commercial strategy.
On 1 January, the airport was separated from the Dublin Airport Authority group, as a precursor to a merger with regional agency Shannon Development.


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How would i go about counting the number of rows that match two variables?
I have a table called: users
and fields called: username & referral
I have another table called: comments
and fields called: *comment_username*
This is the situation, I need to fetch the number of referrals with at least 10 comments (rows in the comments' table) that a specific user has referred.
So i was thinking the code should be something like this crude outline.
$username = 'bob';
$validrefferalcount = 0;
function validreferrals($username){
$referreduser = SQL select * from users where referral='$username';
foreach ($referreduser) {
$numberofcomments = SQL count * from comments where comment_username ='$referreduser';
if ($numberofcomments >= 10){
$validreferralcount = $validreferralcount + 1;
return $validreferralcount;
I apologize for the bad syntax, etc...
Thanks for reading.


text:
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Home ::: Massachusetts ::: Wareham
Population: No data availableSeems like we could not load the Census Data for Wareham, MA (What should have appeared here). Sometimes this happens when we can not properly match a city or hamlet with 2010 census data. Please go back and click on another area. The error has been logged and we will look into the issue.
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Below is a list of the types of businesses in the City of Wareham for which we have Yellow Pages business listings. If you do not see your industry within the list below, adding your business will automatically create it.
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The town of Wareham is located in the county of Plymouth. It covers a total area of 120.0 sq. km. Industries related to aviation, arts and crafts, finance, automotive, construction, commercial printing, information technology, pharmaceuticals, freight forwarding, health and real estate. Commercial spaces available in the town is utilized by garden centers, hardware stores, health clubs and fast food restaurants.


text:
Extreme-ultraviolet lithography systems will not be capable of producing semiconductors with 14-nanometer feature in mass volume by 2014, says Luc Van den hove, CEO of the Imec research consortium. The industry may need to resort to the more expensive method of triple patterning to turn out 14nm chips in volume, he adds.
View Full Article in:Electronics Weekly (U.K.)
Published in Brief:


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11:36 am
Thu June 19, 2014
Early In-Person Absentee Voting Underway
There's been a steady stream of early absentee voters at the Tulsa County Election Board and the Hardesty Library satellite location today. Early voting in advance of next Tuesday's primary is allowed at both sites today, tomorrow, and Saturday, according to Board Secretary Patty Bryant.
The Election Board is at 555 North Denver Avenue, Hardesty Library is on 93rd Street just east of Memorial Drive. Hours at both locations are until 6pm today, 8am to 6pm tomorrow, and 9am until 2pm Saturday.


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Casey Veggies - The Boy
Artist: Casey Veggies
Producer: Hit-Boy, Rey Reel, Haze Banga
Album: None (Promo Single)
Avg Rating: 2.7 ( 7 votes )
Hip-hop heads from coast to coast, beautiful women everywhere… just about everyone seems to "love The Boy." By "the boy," of course, I mean Casey Veggies, who celebrates his sheer animal magnetism on his latest promo single. Hit-Boy, Rey Reel and Haze Banga crowd around the boards on this whip-friendly selection, creating a crisp synth/percussion beat perfect for a Bay Area slapper. Veggies comes with bars to match, delivering his braggadocious bars in a flow that both laid-back and relentlessly on-point. The Boy isn't part of a larger set but, if it leaves you craving more, why not take a(nother) listen to Fresh Veggies, his recent collaborative tape with Rockie Fresh?
Casey Veggies DownloadsStreet BangerClub
Written by richard on Jul 11, 2014
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text:
SIZE .3 oz
Sorry, this product is no longer available for purchase.
Victoriana Nail Lacquer is a textured twinkling vintage pale blue nail lacquer. Things reminiscent of the Victorian era, such as corsets & top hats. 3 Free.
FREE STANDARD Shipping within 2-7 business days within the contiguous U.S. on orders of $75.00 or more.
Most orders are shipped 24-48 hours within receipt of order. Please note that due to the speed of our ordering system, cancellations are not always possible. We ship Monday through Friday. Orders placed on Saturday and Sunday will be shipped the following Monday or Tuesday. We do not ship on major holidays. Orders placed on major holidays will be shipped the next business day.
All orders shipped complete, when all merchandise is in-stock; no partial orders shipped. You will not be charged until your order is shipped.
Please note that any item containing hazardous material, such as nail polish, hair spray, or perfume, is restricted by the U.S. Department of Transportation to ground-only shipping methods.
We are not responsible for orders damaged or missing in transit via a mail forwarder. Please call (866)525-4933 for further assistance.
APO/FBO Addresses: b-glowing proudly ships to our military.
Standard: 2 to 7 business days: $5.95 (For orders over $75, we offer FREE standard shipping)
2-3 Business Day Shipping: + $11.00
1-2 Business Day Shipping: + $20.00
You can confirm delivery of a shipment at www.usps.com using the delivery confirmation number emailed to you at the time of shipping. UPS provides tracking for your orders at www.ups.com.
We share butter LONDON's obsession with color, unbridled creativity, and self-expression—it gives beauty real strength. With shades created for the catwalk alongside the world's top fashion designers, they put you in the front row every day.
butter LONDON has always been fiercely dedicated to bringing you high-fashion color without compromise, so they never add formaldehyde, toluene, DBP, phthalates or parabens to any of their products.
We love the brand's posh sophistication and always use it for high-fashion beauty. You'll love rocking it, no matter your style.
Gorgeous! This makes me happy, and it so far hasn't chipped after 4 days of wear. I love this polish. It's my first of the brand, and I am now officially obsessed! It paints so easily, there is almost no clean-up, and it stays shiny and "freshly painted"-looking for days afterwards. LOVE.
Lovely color, a beautiful blue
This color is incredible! I generally don't go for anything outside of red or pink and fell in love with this right away!


text:
sterling silver 'Hazel' link earrings
designer's notes
With exquisite, handcrafted designs and covetable stones, Rotenier jewelry exudes true elegance in its purest form!
color: Silver Fine jewelry Sterling silver settings Linked loop design Post backs Measures approximately ⅝'' long x ⅜'' wide 14 Day Return Policy; returns must be received within 14 days. No returns will be accepted on international orders. See FAQ for more details. Sterling Silver; U.S.A; style# 323828401


text:
What about those of us who have already purchased all fed ships?
I was wondering what about those of us who have already purchased all ships (in my case everything) the c-store has to offer, we won't be able to get the type 8 shuttle pet. Is there any way to get the pet if you already have purchased the ships? I would appreciate any dev feedback, thanks.
Wishstone says that you can't get it then.
Wow, that is surprising, I get the shaft for being a loyal customer. Way to go customer service and marketing. Thanks.
I understand Floridaguy, I have also bought 5 copies of the game to get all the preorder bonuses, etc along with a lifer subscription. I am a Star Trek collector in RL and I have approximately 500 items. This game and the c-store is an extension of my collecting and I like to have everything that the game has to offer, even if it is just shuttle pet. I certainly hope someone at Cryptic pays attention to this matter. I have been a fan of Trek for over 30 years(I'm 36) and have been collecting for 25 years. Not once since launch have I been unhappy with Cryptic or STO, until now. Just a week or so from that 500 day vet status, very disappointing.
Feel sorry for ya.
I'm not surprised but I would hope they rectify this situation.
( its stuff like this that drove me to no longer purchasing Cryptic stuff)
I can't help but wonder, when this decision within Cryptic was made, did nobody within the team even consider that there would be players who had already purchased all ships, etc from the c-store? I thought about this scenario and I have only had one marketing class in college, now why didn't those within marketing who have (I'm assuming and I'm hoping) marketing degrees reach this same conclusion, that it would honk people off. Especially those people who have dedicated so much of their hard earned money towards this game in support of Cryptic.
Well who knows. Maybe Cryptic will make another way to get it for you :)
|All times are GMT -7. The time now is 10:38 AM.


text:
You are a fan of crusader 20th ? Come to discover the best forums crusader 20th on the internet and share your experience with a community fan of crusader 20th . You can also build a forum crusader 20th and create your online discussion.
A strategic card game taking place in a hand-crafted world, Crusader's Empathy focuses on bringing out the most in the cards.
free, forum, crusader's, empathy, card, strategic, game, canarothian, shadewalker, feyfolk, human


text:
5 bed detached house for sale
£1,250,000 Offers in Excess of
Offers in Excess of £1,250,000
5 bedroom detached house for sale Park Row, Frampton Cotterell, BRISTOLSituated in arguably the most sought after road in Frampton Cotterell this Detached executive home set within a plot circa 1.25 acres. Internally the property comprises on the ground floor Cloakroom, Lounge, Dining Room, Conservatory, Kitchen/Breakfast Room, Sun Room and Utility Room on the first... More details ›
Added on 20/02/2015 by A J Homes , Winterbourne. Call: 01454 560022 Local call rate
5 bedroom country house for sale Mays Hill, Frampton Cotterell, South GloucestershireDating back to the 17th Century, this detached Grade II listed farmhouse was later extended in Victorian times and makes a spacious, elegant family home. Located in an idyllic hamlet setting, it looks over southerly facing walled gardens which lead onto a small paddock/orchard. Approached via a s... More details ›
5 bedroom semi-detached house for sale Perrinpit Road, Frampton Cotterell, BRISTOL, South GloucestershireLocated in the ever sought-after village of Frampton Cotterell is this extended five bedroom semi detached cottage. Set on approximately 1/3 acre, it has the additional benefit of planning consent to convert an outbuilding/barn into a three bedroom detached dwelling (PT14/0636/F). Intern... More details ›
5 bedroom detached house for sale Church Road, Frampton Cotterell, Bristol BS36 2ALBeautifully presented five bedroom detached house, decorated to a high standard in a contemporary style the property comprises a 26´1 x 13´11 sitting room with a fireplace suitable for open fires, dining room, cloakroom, fitted kitchen with solid maple fronted units and integrated appli... More details ›
Added on 23/02/2015 by MacKendrick Norcott, Winterbourne. Call: 01454 560029 Local call rate
4 bedroom detached house for sale 13 Court Road, Frampton Cotterell, Bristol* Rarely Available * Detached Character Cottage * Four Reception Rooms * Four Bedrooms * Conservatory * Commercial Workshop * Utility Room * Off Street Parking More details ›
Added on 10/04/2015 by A J Homes , Winterbourne. Call: 01454 560022 Local call rate
4 bedroom detached house for sale Park Lane, Frampton Cotterell, BRISTOL* Deceptively Spacious * Four Double Bedrooms * Open Plan Dining/Family Room * Study * Utility Room * Beautiful Views to Front & Rear * 90' Rear Garden * Must Be Viewed * Off Street Parking for Several Cars * Ridings Academy Catchment More details ›
Added on 09/04/2015 by A J Homes , Winterbourne. Call: 01454 560022 Local call rate
5 bedroom detached house for sale Beesmoor Road, Frampton Cotterell, Bristol**EXECUTIVE HOME**VILLAGE LOCATION **THREE EN-SUITES** This Executive five/six bedroom family home is located in the heart Frampton Cotterell. It briefly comprises; Lounge, kitchen/dining room, utility room, study, cloakroom, five/six bedrooms, three en-suites, family bathroom, gardens and garage... More details ›
£549,000 Guide Price
Guide Price £549,000
5 bedroom detached house for sale Walter Road, Frampton Cotterell, Bristol, Gloucestershire, BS36A wonderfully presented 5 bedroom executive family home. Newly built just over 2 years ago, this bright and airy home is offered in immaculate condition. Quietly set back, the property can be accessed at both the front, and the rear via the garden which leads directly to the double garage. More details ›
4 bedroom cottage for sale Ryecroft Road, Frampton Cotterell, Bristol BS36 2HPAn exceptional four double bedroom detached cottage that retains original features combined with contemporary living accommodation situated in a sought after location close to local amenities. The original living room has a wood burning stove and oak floor. Moving through to the entrance vestibu... More details ›
Added on 23/03/2015 by MacKendrick Norcott, Winterbourne. Call: 01454 560029 Local call rate
£530,000 Offers in Excess of
Offers in Excess of £530,000
4 bedroom detached house for sale School Road, Frampton Cotterell, BristolGARDENERS DELIGHT! This FOUR DOUBLE bedroom DETACHED family home offers STUNNING LANDSCAPED rear gardens, ENSUITE to master, DOUBLE GARAGE and plentiful OFF ROAD PARKING. More details ›
Thank you.
Your information has been sent to 0 out of 8 agents who have property to sell in the area you specified.


text:
Duracell Doesn't Understand Wireless Charging
Under no circumstances should you need or want to buy one of these things.
Under no circumstances should you need or want to buy one of these things.
The magnetic wireless chargers are also available for older iPhones and last year's Samsung flagships.
Charging cables? Who needs 'em.
Two slick new gadgets and a Lumia-centric software update could make Windows Phone an even more attractive option.
Apple's patent details how it could use near field magnetic resonance (NFMR) to send small amounts of electricity from a computer, laptop or tablet in a metre-wide radius.
A rat's nest of cables at the back of a desk is truly the sign of the gadget-obsessed. But Intel plans to end all that, and soon: it wants your desk—hell, your entire digital life—to be completely wireless by 2016.
There are way, way too many wireless charging standards, which is part of the reason that the technology is struggling to take off. Now, though, Broadcom has developed a chip that could allow your phone to use any of them. This could change things completely.


text:
Aug 13
Neutrino Causing Nuclear Reactions?
I've been a fan of Richard Feynman in that he is one of the few mainstream scientists that actually insisted that theories be consistent with the data, but also because he seemed so capable of thinking a problem through. Recently, … Read More
Posted in Uncategorized Leave a comment


text:
Stock Vector Illustration:
vector pear tree and hand barrow with basket of pears
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- Copyright: beta757
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text:
Days Inn Hannibal
Days Inn Hannibal
Hotel Guest Rating: 6.7/10
4070 Market Street
Hannibal, MO 63401
Welcome to the Days Inn Hannibal
Offering an outdoor pool, complimentary breakfast, Wi-Fi and parking, Days Inn Hannibal also provides Priceline guests an easy location for coming and going to area attractions.
Hotel Features
The low-rise, 63-room property has a seasonal outdoor pool to enjoy in the sunny weather and 24-hour guest laundry facilities to make packing a little easier. All accommodations feature free Wi-Fi and coffeemakers. Many also include mini-fridges and microwaves. There is a free continental breakfast served every morning with an additional hot breakfast bar for plenty of choices. Parking is free of charge.
Hotel Location
Days Inn is located off Route 61, three miles from Hannibal's historic downtown. There are several restaurants about one mile down the road from the hotel. The birthplace of the Unsinkable Molly Brown and Mark Twain's Home and Museum are three miles away, situated close to other Twain sites such as the riverboat cruises and Mark Twain Cave. Rockcliffe Mansion is about five minutes by car. It's three-and-a-half miles to Hannibal Regional Airport.
Guest Favorites
Priceline guests appreciate Days Inn Hannibal's "friendly staff," "clean and comfortable" rooms and "convenient location" to Mark Twain historical sites. Best of all, they also find it "affordable."
Days Inn Hannibal Amenities
- 24-hour front desk
- Accessible facilities
- Accessible parking
- Adjoining rooms
- Air conditioning
- Alarm clock
- Balcony/Lanai/Terrace
- Bus parking
- Coffee/Tea maker
- Coin operated laundry
- Complimentary continental breakfast
- Complimentary in-room coffee or tea
- Cribs
- Desk with lamp
- Free local calls
- High speed internet access
- High speed internet access for laptop in public areas
- High speed internet connection
- Hot breakfast
- Ice machine
- Ironing board
- Jacuzzi
- Meeting rooms
- Microwave
- Outdoor pool
- Remote control television
- Rollaway bed
- Security
- Truck parking
- Wakeup service
- Wheel chair access
- Wireless internet connection in public areas
Hotel Guest Reviews of the Days Inn HannibalOur hotel ratings and reviews are from real hotel guests. Priceline.com ensures that its reviews are guests-only by proactively sending hotel surveys to customers after they've completed their hotel stays. In the post-stay surveys, priceline.com hotel customers are asked a number of questions about their stay and the hotel. On a scale of 1-10 with 10 being the best, the survey asks guests to rate their overall experience and specifically rate the hotel's cleanliness, staff and location. Priceline.com does not allow site visitors to post unsolicited hotel reviews.
September 26, 2014
Good deal for the money
September 4, 2014
Family Traveling with Teens
Nice room at good price. I'd stay again.
August 15, 2014
Senior Travelers
This guest did not leave comments about this hotel.
July 19, 2014
Family Traveling with Teens
The breakfast was served in a very small room with very few seats with a sign stating food could not be taken out. Didn't matter, however as most of the food was gone when we arrived 45 minutes prior to the stated ending time.
July 4, 2014
We thought it was pretty good value for the price. The free breakfast way exceeded our expectations.
June 25, 2014
The hotel was adequate and a good value.
May 24, 2014
May 14, 2014
Family Traveling with Teens
It provided the basic necessities and a hot breakfast in the morning.
April 1, 2014
The room met my needs and expectations for the price that we paid. The staff was courteous and very helpful. Though the hotel is close to the highway, we did not hear any traffic noise.
January 21, 2014
Business Traveler
This guest did not leave comments about this hotel.
(1 - 10 of 32 reviews)
- Holiday Inn Express Hotel & Suites Hannibal (0.1mi)
- Hannibal Inn Hannibal from $52 (0.2mi)
- Super 8 Hannibal from $59 (1.2mi)
- Econo Lodge Hannibal from $69 (2.0mi)
- Quality Inn & Suites Hannibal from $104 (2.6mi)
Most Popular Guest-Rated Hotels in Hannibal
Most Popular Business-Friendly Hotels in Hannibal
Most Popular Family-Friendly Hotels in Hannibal
- Best Western Plus On The River
- Quality Inn & Suites Hannibal
- Holiday Inn Express Hotel & Suites Hannibal


text:
President Bush:"Americans understand that the price of crude oil is going up but what they don't want and will not accept is manipulation of the market and neither will I."
Bush is calling on the federal trade commission to investigate the possibility of unfair price manipulation. The president also ordered a temporary halt to oil deposits destined for the nation's strategic petroleum reserve. However, critics of Bush's plan to halt oil deposits claim the move will NOT affect gas prices.
Another White House proposal includes revoking $2 billion in tax breaks for oil companies. The tax breaks are part of an omnibus energy bill signed into law by the President last August.
On Capitol Hill, Senate Republicans are suggesting a $100 fuel-cost rebate for millions of taxpayers. Democrats countered that the $100 gas rebate proposal is coupled with a renewed call for oil drilling in the Alaskan Wildlife Refuge, a provision that likely will sink the bill. Instead of gas rebates, Democrats offered their own plan to suspend the 18.4 cent federal gasoline tax for two months.
Yet another plan drafted by Democratic Sen. Byron Dorgan of North Dakota is calling for a windfall profits tax on oil companies. Dorgan's plan comes at a time when the Exxon Mobil Corp. is posting 1st quarter profits of $8.4 billion -- the 5th largest quarterly profit for a public company in history. While Exxon executives claim the company has no control over rising prices, some politicians in Washington disagree.
Sen. Robert Menendez,(D) New Jersey: "Last year the big oil companies hiked gas prices and blamed an act of god. But it's crystal clear that the current spike in gas prices is at least partly due to an act of greed."


text: Quoting myself from an year ago: (19 October 2004) -------------------------------------------------------- Hi all, Linux TCP/IP protocol stack is well built but it lacks a bit in performance. To give you an idea, my box has a 10MBit connection to the internet, and if I try to transfer a file to a little lagged but high bandwidth host (50ms / 100MBit) this lack of optimization will begin showing. Even if my link will permit to transfer from this host at about 900-1000KB/s I will get only 600-650KB/s. Even using windows tuned to broadband connection I will get 900KB/s. This seems to be related to the tcp window scaling, and even if i try to tune the kernel related parameters (receive/send buffer) logging the traffic the tcp window seems to be left too small for this transfers. Web100 (www.web100.org) is a project that has solved this issue creating a patch that will tune every tcp/ip connection to get the best performance. I hope this patch will be inserted in the fedora stock kernels. Regards, Angelo ----------------------------------------------------- In a year not a single reply.. i'm still hoping to get this in Any idea, comment, reason to disagree, something ? Regards, Angelo


text:
Chicagoland Entrepreneurial Center
Watch breaking news videos and read news updates about Chicagoland Entrepreneurial Center on Crain's Chicago Business
William Blair makes VC, startup play
banking firm recently hired Jim O'Connor Jr., a venture and private equity investor and chairman of the Chicagoland Entrepreneurial Center, which operates 1871. The 48-year-old joined William Blair late last year after leaving the Chicago
Matt Maloney gets tied up in a fashion faux pas
The co-founder of GrubHub wore a tie (gasp!) at the techy Momentum Awards party.
Tech community steps out to honor Segals at Momentum Awards
successful entrepreneurs often make rookie mistakes. While being honored for their accomplishments by the Chicagoland Entrepreneurial Center, Carole and Gordon Segal, founders of retailer Crate & Barrel, surprised the crowd with a couple of
How Howard Tullman is shaking up 1871
home to nearly 250 entrepreneurs and start-ups. It's staffed by about a dozen people and run by the Chicagoland Entrepreneurial Center. The idea behind its founding was to give Chicago's start-up tech scene a focal point, and to address
Ryan, Ferro to donate $4 million to launch Northwestern incubator
university." In addition to being Northwestern trustees, Messrs. Ferro and Ryan have been active with the Chicagoland Entrepreneurial Center, which played a critical early role in helping Chicago's startup tech community get rolling over the
This week in tech: 3 events worth checking out
founder Adrian Holovaty and entrepreneur and investor Rishi Shah are getting out their crystal balls for the Chicagoland Entrepreneurial Center's annual startup forecast Thursday morning at Chase Auditorium. Also on the ticket is Greg Goff, chief
1871 gets down to business
Kudos to the Chicagoland Entrepreneurial Center for picking a businessman to run its startup incubator, 1871. Notice I said "businessman," not "entrepreneur
Howard Tullman takes over 1871
job and the accompanying lead role at the Chicagoland Entrepreneurial Center with the backing of both Mayor Rahm Emanuel ..... Mr. Tullman became a candidate after the Chicagoland Entrepreneurial Center, the not-for-profit that runs 1871
If social media can fuel murders in Chicago, it can help end them, too
must change. I'm calling for just one or two smart people to form a social-justice startup at the Chicagoland Entrepreneurial Center's 1871. Surely there is one person of the 2.7 million who live in Chicago who can create an app that
These sharks feed in a tent at Millennium Park
How do tech entrepreneurs and investors kill time between dinner and dessert? Evaluate startups. The Chicagoland Entrepreneurial Center added its own version of "Shark Tank" to its annual Momentum Awards last night, with three startups
CEC now hopes for new boss by Thanksgiving
The Chicagoland Entrepreneurial Center is still looking for a replacement for Kevin Willer. The nonprofit group, which is a resource for startups and is perhaps best
How not to network
Networking isn't merely tolerated at the Chicagoland Entrepreneurial Center's annual Momentum Awards dinner. It's built into the schedule. The evening begins with an hour of networking that, for entrepreneurs
How to turn more startups into growth companies
Bow Truss Coffee; Jeff Malehorn, CEO of World Business Chicago; Jim O'Connor, co-founder of the Chicagoland Entrepreneurial Center and managing director of investment firm MVC Capital; and Howard Tullman, founder of Tribeca Flashpoint
Startup Psych 101: Embrace risk – and don't fear failure
information technology investment firm. He helped create the Illinois Venture Capital Association and the Chicagoland Entrepreneurial Center, and he was a key backer of 1871. He's widely seen as a cheerleader for Chicago's entrepreneurs, but he tries to keep
Why aren't more women in Chicago tech?
technical founders. (Starter League is an option here, but a pricey one. It would be interesting to see the Chicagoland Entrepreneurial Center provide scholarships for female business owners interested in learning technical entrepreneurship.) We


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New Berlin voters choose between five candidates for mayor in the Feb. 19 primary.
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- Boon to Muskego downtown planning expected to come soon
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- Memorial Day to be honored in Muskego, New Berlin
- Muskego man charged for shooting threat (1)
- In Brief: Church, football and Moorland Road


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Thank you for helping to make the opening of the Silver Line a success. Nearly 220,000 trips were taken to…Continue Reading →
A family waves their Silver Line grand opening pennants and commemorative SmarTrip cards while riding one of the Silver Line…Continue Reading →
Until the Silver Line opens at noon on Saturday, July 26, Fairfax Connector bus service will be available to all…Continue Reading →
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The introduction of the Silver Line represents a significant service increase for Metro. Once the line opens, Metro will run…Continue Reading →
Fare machines and SmarTrip card dispensers are installed at the new McLean Station. In the final days before the Silver…Continue Reading →
Looking out from Greensboro Station, the Silver Line is the newest partner in the business community. Visit a SmartBenefits® seminar…Continue Reading →
Metro plans to release a new SmarTrip card on July 26th to commemorate the opening of the Silver Line. The…Continue Reading →
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Building bright Mediterranean flavors, one tweak at a time
On a menu full of appealing pork- and beef-centric dishes at the Rumpus Room downtown, it was the octopus salad that leaped out.
Such clean, nimble flavors - this was an irresistible dish, light and bright on the palate. True, the meat dishes have clear, big flavors, too; nothing is muddled on this menu. But that salad, with its herb and citrus note and contrasting textures, is compelling. »Read Full Article(1)
Twice the wine dinner: c.1880 doubles presentations, pairings on Dec. 30
Journal Sentinel files »Read Full Blog Post
That Facebook thing: 10 books that stayed with me
My friend Dave Roidt tagged me on a Facebook thing that's going around: 'list 10 books that have stayed with me in some way." For you Dave, I will comply.
Actually, I'm happy to do it. I enjoy reading these things when other people do them. I like to find out what people actually read for pleasure and inspiration, as opposed to what books they feel obliged to serve up in a canon-making exercise. »Read Full Blog Post
Top of the Charts: Eminem bounds to No. 1
After last week's viral anomaly that raised Miley Cyrus' "Wrecking Ball" back to number one on the back of a YouTube comedian's parody video, things are back to normal on the Billboard Hot 100. After steadily climbing in recent weeks, Eminem finds himself on top of the charts. See the video for his big hit song below, then jump back in time to see the biggest hits this week in 2005, 1995, 1985 and 1975.
No. 1 hit Dec. 15, 2013 »Read Full Blog Post
On Tap Today: Sleighriders at Shank and holiday lights
The annual Sleighriders show at Shank Hall kicks off the holiday season with good music and does it for a good cause. It's at 7 p.m. tonight.
Or cruise the area to see some spectacular holiday light displays. They'll make you forget it's Monday. »Read Full Blog Post


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By LARRY CHAMBERS, Staff
INDEPENDENCE — A Galax man was indicted on five charges in connection with a fire that damaged Pine Crest Apartments in the city.
A Grayson County Circuit Court grand jury last week indicted Phillip Allen Holdaway, 34, on two counts of arson, two counts of statutory burglary and one count of felonious damage to property.
By LARRY CHAMBERS and DON RICHESON, Staff
INDEPENDENCE — Members of the Grayson Citizens for Better Schools staged a peaceful demonstration Monday morning in front of the Grayson County School Board office in Independence, calling for changes in leadership and protesting recent budget cuts and teacher firings.
Several motorists honked their horns as they passed by the group, which held signs referencing school leaders they'd like to see replaced.
HILLSVILLE — To avoid the strain of higher water and sewer costs for town citizens, Hillsville officials are discussing options with the Carroll County Public Service Authority (PSA) to bring additional wastewater through the town's plant in order to maximize use and prevent a potential price hike.
Travis Jackson, a consultant for the town, along with Mayor Greg Crowder and Town Manager Retta Jackson, approached the Carroll PSA at its April 13 meeting, and requested specifically that the town treat wastewater flow from Interstate 77's busy Exit 14.
Clean it up
I am a citizen that lives on Tower Road and it is nothing but a trash pile, especially the end coming toward the four-way stop off Country Club Lane. It seems like people just want to throw their trash out all over the road. It looks awful and terrible and needs to be cleaned up. I would like to live in a nice, clean area. Please, if there is anything you can do about this, please help.
INDEPENDENCE — Michael Hash has announced that he will seek the Republican nomination for the Providence District seat on the Grayson County Board of Supervisors.
"I understand the needs of our community," said Hash in a news release. "I am always looking for ways to serve my community and I am very excited about the opportunity to serve the residents of my districts."
John Brewer, who holds the Providence seat now, has said he will not seek re-election. Instead, he is running for a school board seat.
These profiles of vendors at the Independence Farmers Market were submitted by market manager Michelle Pridgen.
INDEPENDENCE — If you have been to the Independence Farmers Market in Independence Town Park, you may have noticed a booth with a large "Grayson LandCare" banner behind it, potted plants in front, and a very friendly woman in between. Kathy Cole, newly-elected president of Grayson LandCare Inc., has set up a fund-raising booth for the past three years.
ABINGDON — Robert Donald Morgan III is scheduled for a preliminary hearing on May 13 in federal court, after being charged with one count of bank fraud, according to police reports.
INDEPENDENCE — A 2012 incident in Grayson County schools still causes concern for many parents, and a letter obtained by a citizens' group shows those concerns were shared by the sheriff's office, as well.
The situation involved a Grayson County High School student who allegedly made a "homicidal threat" to another student and was reportedly seen with a knife at school, according to a letter from Sheriff Richard Vaughan to Grayson Schools Superintendent Kevin Chalfant on Nov. 8, 2012.
RICHMOND – Dr. Dean E. Sprinkle of Statesville, N.C., will become the next president at Wytheville Community College, announced Glenn DuBois, chancellor of Virginia's Community Colleges. Sprinkle will begin his new post July 1, replacing retiring president Charlie White.
"Dr Sprinkle will be a tremendous fit for Wytheville Community College," DuBois said in making the appointment. "He has built an impressive career of academic leadership and I'm looking forward to seeing the next chapter of that unfold here in Virginia."
You hear a lot this time of year about the stress students are being put through as they take state tests; about the anxiety and sleepless nights as they worry about their future.
This year, in Grayson County, the teachers and staff are the ones concerned about their fates as the school system tries to find a way to balance a budget while faced with a funding shortfall.
Copyright © 2015
The Galax Gazette is your source for local news, sports, events and information in Galax, Grayson, Carroll, and surrounding areas.


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Wagner, W.M., P.G. Wright, and H.E. Stokinger. 1964. Inhalation of toxicology of oil mists. I. Chronic effects of white mineral oil. Am. Ind. Hyg. Assoc. J. 25:158-168.
Waldron, H.A. 1975. Proceedings: The carcinogenicity of oil mist. Br. J. Cancer 32(2):256-257.
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17 September 2012 17:19 [Source: ICIS news]
HOUSTON (ICIS)--INEOS Nitriles' Green Lake acrylonitrile (ACN) plant is back in operation and producing at its planned rate, the company said on Monday.
The plant near Port Lavaca, Texas, was struck by lightning on 4 September and lost power. Plans had called for the plant to return to operation by 11 September.
The plant has a capacity of 545,000 tonnes/year of ACN, according to INEOS Nitriles.
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Officials confess to lack of correct information on hotels and bars
Licence renewal process runs into trouble
High-level committee put in place to study issue
HYDERABAD: The Greater Hyderabad Municipal Corporation (GHMC) is yet to have complete data on the trade licences issued to hotels, restaurants, lodges, bars and others in the 12 municipalities even as its policy prevalent in the erstwhile MCH area comes under the High Court scanner.
Officials confessed that correct information on hotels and bars was not available since trade licence records have not been computerised in the suburbs. For the core area itself, GHMC has been grappling with the issue ever since the court had ordered it to act tough against hotels, bars and others not providing parking space and functioning from residential properties. After identifying 20-odd trades which cause traffic and sanitation problems, it had suspended automatic renewal of licences and had asked the businessmen to furnish no objection certificates from the police and town planning wing. In the meantime, about 20 per cent of establishments had already got their licences renewed.
The health and sanitation wing had put 6,700 others in abeyance. Licence renewal was to be applied for in the head office and report from the assistant medical and health officer concerned was to survey and report. But this ran into trouble when the head office had cleared close to 1,000 applications under controversial circumstances sans a survey report.
GHMC's top boss C.V.S.K. Sarma put an end to all the processes and put in place a high level committee headed by the Additional Commissioner (Planning & Projects) K. Dhananjaya Reddy and consisting of Additional Commissioner (Health & Sanitation) R. Ram Mohana Rao, Chief Veterinary Officer P. Venkateswara Reddy, Chief Medical and Health Officer (CMOH), DCP (Traffic) to study the issue.
The panel is to submit a report after looking into the parking space, building usage and whether the business was under the bulk garbage system for all the 20 trades. Already, GHMC had issued closure notices to over 3,000 hotels and others for functioning illegally from buildings meant for residential purposes.
Task cut out
Considering the trouble it was going into renewing trade licences under stringent norms for the core area, GHMC has its task cut out when it looks beyond and into the suburbs which are witnessing no less growth.


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BOSTON ( MainStreet) -- "Green" technology can turn the hot weather baking much of America this summer from a drag on your home's air-conditioning bill to a source of free solar energy -- if you know what improvements to make.
Sachs estimates homeowners can recoup the costs of carefully chosen energy improvements over five years -- and enjoy a much more comfortable indoor climate in the meantime.
"By bringing your home's energy systems under control, you'll get rid of drafts, kill a lot of equipment noise and eliminate rooms that are too hot or too cold," he says. "That's worth a lot right there."Here's a look at five high-tech moves the expert believes every homeowner should consider this summer to maximize their property's energy efficiency: Cost figures are estimates for a typical four-bedroom home and were provided by Complete Home Evaluation Services, a Maine energy-efficiency consultancy. Get a home energy audit
Cost: $200 to $750
Sachs recommends getting a "Home Energy Rating System" review or other comprehensive audit of your property's fuel efficiency before even thinking about installing a solar panel or new air conditioner. "Instead of just blindly throwing money at energy efficiency, you want to invest in a consultant with the right tools and get the right 'road map' of what to do," he says. Home-energy audits typically take around a half-day and involve checking your home's heating and cooling systems, as well as using high-tech infrared heat detectors and negative-air-pressure machines to find gaps in your property's insulation. Audits typically cost a few hundred dollars, but your local utility or state energy commission will sometimes offer them for free or at a reduced cost. Fix your home's "envelope"
Cost: $4,000 to $5,500
Once you've done an energy audit, you should fix any gaps the review has uncovered in your home's "envelope" -- the insulation that separates heated or cooled living spaces from unheated attics or the outdoors.


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Thread: Muttonhead's Newborn
July 8th, 2009 #1
Muttonhead's Newborn
Here is the pencil drawing for my entry.
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July 8th, 2009 #2
This is an awesome illustration. I love all of the details you put into your pencils. I'm just having a little bit of trouble understanding the connection to "newborn". Is this a freshly created monster?
July 8th, 2009 #3
yay i like that! cool monster
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July 8th, 2009 #4
holy cow,that is some insane sketching there, killer pic will be waiting eagerly for the next stage
July 8th, 2009 #5Registered User
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wow this can even be final the draftsmanship is so good! looking forward
July 8th, 2009 #6Registered User
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wow incredible penciling really really nice.
I dont know how it connects to newborn either, but who cares, this is incredible.
July 8th, 2009 #7
the architecture looks awesome, nice detail. I love the creature as well, the tentacles seem a bit too short, but it makes sense being a newborn creature. This is my fav image so far, good work..
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cracking piece of drawing!
July 8th, 2009 #9
I love that creature and composition.
This is phenomenal, congratulations on winning!
Hee hee.
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July 8th, 2009 #10
That is really beautiful pencil work.Nice.Very Very exceptional pencil skills.
My crit thoughts so far:
1. the creature looks as tho he has flopped down onto this building, yet the bricks/pieces flying up by his head don't look like they used to belong where his head is now, they kind of look like they are random pieces that are flying up and out from his head. i think maybe move them down,change their angles, or move them forward to the right.. if you get what i mean, so they look like they were pushed from their spot by his face ( if that was what you were meaning)
2. i dont know if you want the building to look like it is leaning from his weight, but it does.Which is really good, i think. However, some of the front where it is still rather straight should be popping off,cracking,creaking, at the face of the building, from the strain, don't you think?
"Steering perception? i am.
Inviting contradiction? i am."
July 8th, 2009 #11
Far out dude! very cool pencils. I cannot wait to see this thing popping out with some values either in grayscale or colour.
Keep the child alive...
Dare to Believe Sb
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July 9th, 2009 #12
July 9th, 2009 #13
Muttonhead, that's incredible pencil work. Strong Composition!
July 9th, 2009 #14
Wonderful work Muttonhead. Superb pencil control, with great drawing underneath. It has the WOW factor for me.
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July 9th, 2009 #15
Nickillus - Thanks a bunch!
Artboy - Thanks! I owe a debt of gratitude to the inventor of the .3 lead pencil
Justice Von Brandt - Danke Schon
comixnut - Color is planned, indeed. What I am wrestling with now is whether to ink this up with pen, then do color, or just go straight to color with the pencil.
riotorange - Thanks man. The idea was that he actually appeared there, or maybe even grew from a small thing into a big thing in that place, so he therefore displaced parts of the building as he grew. I love your ideas about the strain on the building, and I will try to incorporate those.
Cryptcrawler - Haha, well there are a lot of great entries so far, so I have my work cut out for me, but thanks for the vote of confidence! I just liked the concept for this contest and was inspired!
pearlyking - Many thanks!
ROBOT_TANK - Thanks man!
Bhrandon - Thanks. See my answer to FaylneVarger below.
NoriRoll - Thank you! Yeah, I love the look of pencil drawings, especially those of Beksinski, Alan Lee and John Howe, they seem like finished pieces to me too!
AhChu - Thank you!
R a n d i s - Thanks!
FalyneVarger - OK, so the idea is that (and I realize this is perhaps too subtle ATM, I have a plan for making it clearer) the little dudes on the walkway (which are hard to see at this DPI), have summoned their God, which they think is going to look like the little statue guy (note the multiple eyes and arms), but instead of a kindly looking ocotopus-man, they get this, which is their God's true form. I figured it is "newly born unto the Earth", which maybe is a stretch, but I wanted to do something other than an actual baby. He is new to THIS world, which is a birth of sorts, no?
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July 9th, 2009 #16
looks great mang! love the detail! cant wait to see the finish!
July 9th, 2009 #17
goddamn... i should just give up now. that is just fucking killer.
you've got my vote already man.
July 9th, 2009 #18"Steering perception? i am.
Inviting contradiction? i am."
July 9th, 2009 #19
I am crazy about this drawing. So many wonderful details. Everytime I come back to look at this I see something new. I love the little figures on the stairs that are somehow attacking/helping the monster.
I have to say the really amazing part with this is the fact that the statue and the monster are mirror images of each other. Not sure if it was made after this people the statue is of and something went wrong but it really has a nice twist to it and would love to hear your story behind it ^^
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July 10th, 2009 #20
I'm always impressed by your linework but this IMO is your best to date. It almost doesn't need colour but I'm sure you'll do a cracking job. One thing, you say that he grew in the building - I think it would be better if he had just fallen straight down from the heavens in response to a prayer. You could have some of the little guys gathered round the statue, going Oh shit, what have we done!? I just think the idea of that thing suddenly falling from the sky is pretty funny, but that's just me
BTW - This would make a great cover!
July 10th, 2009 #21
The draftsmanship is just unbelievable. I seriously cannot critique this.
July 10th, 2009 #22Registered User
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oh holy fuck.
July 12th, 2009 #23
SmokenMirrors - Thanks!
DirtSyndicate - Thank you!
riotorange - thanks man, I have a few ideas of how I could make the image a little clearer on what is going on. Thanks!
LillyCat - Yep, the statue is deliberate. It represents what the followers of this God THINK their God looks like, but in fact, the reality is much more horrifying.... not to mention way bigger. I am flattered you like the piece!
Baron - Yeah, that would perhaps be funnier/make more sense.... lemme think about it. You are too kind sir. Thanks a bunch!
ninjacat - Thank you!
Harris-ejaz - Well, that's putting it mildly I suppose! Thanks!
OK, here is an update. Quick underlay of colors... lemme know what you think of the color scheme:
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July 12th, 2009 #24
Lovely work. Would love to see the castle/palace in a complementary colour, like a greenish tone... Not sure that is possible to do proper but I think it would "pop" the painting..
July 12th, 2009 #25
goddamn... wow man. this is gonna be KILLER
i cant wait to see it finished! great color palette man. i love the light coming up from the cracks in the ground, brilliant.
July 12th, 2009 #26
July 12th, 2009 #27
This is great work. A real inner vision informs everything you do - the surface style and colour are wonderful, but what really makes your stuff stand out is that inner vision thing.
I can't wait to see this on the Finally Finished section. Wonderful work.
From Gegarin's point of view
July 12th, 2009 #28
Great drawing man.
Now you have a tuff thing to do on the color, so that you dont just overpaint all of the nice drawing.
Looking forward to see more.
If the color becomes as great as the first picture than its devinately one of my favos.
July 12th, 2009 #29__________________________
July 12th, 2009 #30Registered User
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This is just fantastic. I didn't get it at first but having read your explanation I love the concept as much as the drawing. You might be able to really send your point home if you color the statue more or less the same as the monster (even if it's faded and worn looking).
I'm inspired.


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The Comedy In Fifty Shades of Grey
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The Oath | The second installment in a planned trilogy on American policy post–9/11—which follows her strong 2006 ND/NF nonfiction entry on Iraq, My Country, My Country—Laura Poitras's extensively researched, intelligently structured doc centers on Abu Jandal, a taxi driver in Yemen who was once Osama bin Laden's bodyguard. As the intensely magnetic Jandal describes his time in Al Qaeda, the spectral presence of his brother-in-law, Salim Hamdan, OBL's former driver, then still locked away in Gitmo after being the first detainee tried before a military tribunal, constantly looms over Poitras's quietly forceful indictment of the "War on Terror."
The Red Chapel | A (purposefully?) insufferable Danish journalist, Mads Brügger attempts a Borat-like stunt in Pyongyang when he convinces the North Korean government of the cultural value in hosting the fart-filled slapstick revue of Danish-Korean performing duo Simon and Jacob. Under 24-hour surveillance in a police state, Brügger's attempts to "expose the very core of evil that is North Korea" are impossible to achieve, of course; the real draw of this unclassifiable doc is his two charges' increasingly conflicted feelings—about Brügger, his deceptions, and their faux-"homecoming." Or, as Simon responds with Scandinavian calm when his director pushes them too far: "The fondue pot has boiled over."
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Mulyani WaghrayTwitter Statistics
@villagourmet - Tracking since September 21, 2009
AdleenaAzmy okay i m like fuckin scared i told my mom bro about it and she said tomorrow i will go and meet that teaxhe
Salvador, Brazil
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Daily Sales Up to 70% OFF Limited-time deals start every day at 12 pm ET.
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(Previously My Favorites) My Feed
My Wayfair
Shop looks from your favorite magazines
All Season Luxurious Cotton Blanket
by Simple Luxury
Skipjack Ocean Crest Cotton Throw Blanket
by Southern Tide
Oasis Cotton Throw
by C & F Enterprises
Polka Dot Stripe Cotton Throw
5 stars
Cute throw. I wanted to use this as a spring decoration. The colors are just as advertised. Pros: Well made. Bright Eastery colors. Nice size for a throw or to wrap up a baby. Cons: None.- Mindy in Hooper, UT
Peach Couture Signature Cashmere Throw
5 stars
Gave this throw to my sister for her 50th birthday. She loved it!- Leigh in Dunwoody, GA
Jewel Cotton Throw Blanket
5 stars
I believe my headline states it all - high quality, very well made and beautiful- Darlene in Florida
Betsy Woven Throw Blanket
4 stars
I bought this in red and it looks great with black furniture- Jana in Amarillo, TX
Cori Dantini Sweetness And Light Polyester Fleece Throw Blanket
5 stars
I matched this with red and yellow color skeins and gray furniture- Jana in Amarillo, TX
Oh So Soft Super Soft Throw Blanket
5 stars
This is truly one of the softest blankets I've ever felt in my life! It washes wonderfully with very low lint! I snuggle with it almost every night !- AMY in Largo, FL
Grand Hotel All-Natural 100-Percent Cotton Basket Woven Blanket
5 stars
Could be the best blanket l have ever bought light but wram- Joseph in Hoover, AL
Scotch Chenille Viscose Throw Blanket
5 stars
I used this throw many times this past winter to fight the chill. Comfortable and attractive.- David in Coatesville, PA
Mink Polyester Fleece Blanket
5 stars
The item was very soft and also liked the pattern. Pros: The quality and design was nice.- James in Las Vegas, NV
Ibiza Cotton Throw Blanket
5 stars
This throw is beautiful. The colors make you feel very clean and fresh! It will look so lovely on my new couch I will be ordering in off-white. It is of excellent quality. The weight of it is not- Mom in Weed, CA
Triple Rib Electric Heated Warming Blanket
5 stars
Machine Washable...... great for home with pets. Blanket is not warm to the touch but rather heats in spots that it touches....- Tiffany in Grand Rapids, MI
Canyon Decorative Woven Acrylic / Polyester Throw
5 stars
I love this throw to curl up on the couch and have a quick nap. It's big enough and feels so good.- Linda in Cottonwood, AZ
Checkered Polyester Fleece Throw Blanket
5 stars
Longer than the usual throws and very well made. Washes beautifully.- Donna in Solvang, CA
Mink Greek Key Textured Sherpa Throw Blanket
5 stars
This throw is so comfortable and warm enough for cold weather. Can't beat the price too! Highly recommended!- Fenny in San Jose, CA
Microlight Plush Throw Blanket
5 stars
This throw blanket turned out great! It is exactly as it is described online and the quality is good! I would not have any issues recommending it to others!- L in NY
Down Alternative Blanket with 3M Scotchgard
5 stars
Perfect year round weight. Color and fabric did not disappoint. I would recommend this blanket highly. Pros: Light weight but wae- Marnee in Punta Gorda, FL
All Season Down Alternative Reversible Blanket
5 stars
Summers in the south are hot and humid. This satin-like coverlet is awesome to sleep under on those nights when one just can't get comfortable. The coolness of the satin on the skin makes one drift- CAROL in ROLAND, AR
Rose Perfume Spring Rose Cotton Throw Blanket
5 stars
Great for a sea in my room , great quality. I am happy- Amy in Miami, FL
Flannel Polyester Throw Blanket
5 stars
Bought one for my daughter and for my son. They both love how plush the blankets feel!- Lucinda in Delaware, OH
Coastal Throw Blanket
5 stars
Great blanket to cuddle up with and read a good book!- Jamen in Old Hickory, TN


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September 14 - 16, 2015
Monday/Tuesday September 14, 15: 7:30am - 5:00pm
Wednesday, September 16: 7:30am - 4:00pm
Lunches provided daily
Course Content (subject to change):
Day 1
Tools of the Trade - Basic Physics and Instrumentation: Keith Fujioka, BS, RVT, FSVU
Neuroanatomy Basics: Aaron Stayman, MD
Vessel Identification: Fujioka
Transcranial Doppler Technique: Fujioka
Transcranial Imaging Technique: Colleen Douville, BA, RVT
Hemodynamics/Waveform Morphology: Douville
Demonstration - Transcranial Doppler: Fujioka
Demonstration - Transcranial Imaging: Douville
Guided Hands-on Session - Transcranial Doppler and Imaging
Day 2
Collateralization Effects of Carotid Disease : Fujioka
Recognizing Cerebral Emboli: Krislynn Barnhart, BS, RVT
TCD Emboli Detection: Clinical Significance: Stayman
Monitoring with TCD - Carotid Stents: Paul P Huang, MD, MSc, FACC, FSCAI, RPVI
Subclavian Steal and Intracranial Effects: Fujioka
Mechanical Compression of the Vertebral Arteries: Fujioka
Subarachnoid Hemorrhage & Vasospasm: Pathology & Pathophysiology, David Newell, MD
Functional Testing - The CO2 Challenge (with demonstration): Douville
Guided Hands-on Session - Transcranial Doppler & Imaging
Hands-on: Monitoring headgear set-up
Computer Learning Stations - Hemodynamic Software
Day 3
Intracranial Stenosis & Occlusion: Acute Stroke: Douville
Utilization of TCD in Sickle Cell Disease: Douville
TCD/I in the Detection of Vasospasm after SAH: Douville
TCD Monitoring: Carotid Endarterectomy: Douville
TCD/I in Raised Intracranial Pressure & Cerebral Circulatory Arrest: Arthur Lam, MD, FRCPC
PFO Anatomy, Pathology & Treatment Strategy: Huang
PFO TCD Protocol and Interpretation Criteria: Meryl Harley, BA, RVT
Demonstration - PFO Testing: Joshua Renz, BS, RVT & Harley
Guided Hands-on Session - Transcranial Doppler & Imaging
Computer Learning Stations - Hemodynamic Software
TCD Case Study Poster Presentations
Discount rate for booking may be available, but not determined at this time.
Visit our website at http://www.silvercloud.com/seattlebroadway
How to make a Reservation Via Phone:
Call the hotel directly at (206) 325-1400 or Toll Free at (800) 590-1801
** If you need to book guest rooms outside of the
group event dates, please contact the hotel directly.**