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Genetic Counseling Center - Federal Way, WA
Federal Way Geneticist, Ph.D. Doctors for Genetic Counseling
Type of Physician: Geneticist, Ph.D.
What is a Geneticist, Ph.D.?
A certification by the Board of Medical Genetics; practitioners work in association with a medical specialist, are affiliated with a clinical genetics program, or serve as a consultant to medical and dental specialists.
Specialty: Genetics: Medical (Ph.D.)
Common Name:
Medical Geneticist Doctors in Federal Way *
Group Health Oncology Clinic
Center On Human Devel & Disabil
Center On Human Devel & Disabil
University Of Washington GEN
Center On Human Devel & Disabil
Center On Human Devel & Disabil
Center On Human Devel & Disabil
University Of Washington GEN
Seattle Childrens Hospital Gen
Seattle Childrens Hospital Gen
Seattle Childrens Hospital Gen
Longevity Medical Clinic
Federal Way, Washington
Genetic Counseling
What are genetic professionals and what do they do?
Genetics professionals are health care professionals with specialized degrees and experience in medical genetics and counseling. Genetics professionals include geneticists, genetic ...
Other Related Genetic Counseling Articles
Emergency Contact for Federal Way
Nearby Federal Way Hospitals *
Saint Francis Hospital
Auburn Regional Medical Center
Mary Bridge Children's Hospital
Tacoma General Hospital
St Joseph Medical Center
Allenmore Hospital
Good Samaritan Hospital
Madigan Army Medical Center
VA Puget Sound Healthcare
Highline Medical Center
Valley Medical Center
Highline Specialty Center
Regional Hospital
St Clare Hospital
Schick Shadel Hospital
Western State Hospital
St Anthony Hospital
Saint Elizabeth Hospital
VA Puget Sound Healthcare Seatt
Harborview Medical Center
Swedish Medical Ctr Cherry Hill
Swedish Medical Center
Virginia Mason Medical Center
Group Health Central
Overlake Hospital Medical Ctr
Harrison Medical Center
University Washington Med Ctr
Seattle Children's Hospital
Kitsap Mental Health Services
Swedish Medical Center Ballard
Bremerton Naval Hospital
Kindred Hospital Seattle
Northwest Hospital
Evergreen Health
Fairfax Hospital
Harrison Silverdale Hospital
Lakeside Milam Recovery Center
Providence St Peter Hospital
Snoqualmie Valley Hospital
St Peter Chemical Dependency
Capital Medical Center
Swedish Edmonds Hospital
*Provider Directory Terms of Use:
The WebMD 'Provider Directory' is provided by WebMD for use by the general public as a quick reference of information about Providers. The Provider Directory is not intended as a tool for verifying the credentials, qualifications, or abilities of any Provider contained therein. Inclusion in the Provider Directory does not imply recommendation or endorsement nor does omission in the Provider Directory imply WebMD disapproval.


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Kbr CompanyGurgaon, Haryana
Products & Services
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Company Profile
KBR's India office, located in the vibrant city of Gurgaon, Delhi, has been an important asset to KBR since its opening in 2008. Although KBR has operated in India since 1974 delivering key grassroots and revamp projects in ammonia, refining and petrochemicals, the India office is home to technical sales and a growing global engineering center.
We specialize in process development and technology licensing of energy-efficient and cost-effective process ...view all
Fact Sheet
|Year of Establishment
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More than 1000 People
More than Rs. 500 Crore ( or More than US$ 100 Mn Approx.)
Contact UsKbr Company Jaspal Singh09921420948
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Send your enquiry to this supplier

subset: commoncrawl


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Welcome to SANTA MONICA SUVS,
purveyors of finest pre-owned land rovers
At our dealership in Santa Monica, our main goal is to keep you satisfied with your pre-owned car for years to come. Because we know you have amazing taste, we know you will appreciate our selection of high-quality vehicles. We are so confident that you will fall in love with each model in , whether it's the land rover range rover-sport-discovery-defender90 and more, Our staff is so proud to serve you and the Los Angeles area since 1989 , and are eager to assist you in every step of the car buying process. Don't hesitate to contact us by phone oe email with all your questions.
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UFC Q&As aren't usually the place to discuss serious issues, but one curious fan made things interesting this past week.Filed under: Featured Videos, News, UFC, Videos read news >>
Alistair Overeem is a busy, busy man, so to say he's waiting for the phone to ring wouldn't exactly be fair. That said, he is looking forward to receiving his next assignment from the UFC, and he's h read news >>
UFC heavyweight Alistair Overeem delves into the reasons he believes Mirko Cro Cop won't request a rematch against him. read news >>
ADELAIDE, Australia – Ever wonder how good Alistair Overeem is in the kitchen? Well, wonder no more. Filed under: Featured Videos, News, UFC, Videos read news >>
Prior to the UFC Fight Night 65: Miocic vs. Hunt official weigh-in on Friday in Australia, Alistair Overeem, Jessica Eye and Michael Chiesa fielded questions from fans. read news >>
UFC heavyweight Alistair Overeem, along with Jessica Eye and Michael Chiesa, will be the featured guests for the UFC Fight Night 65 Q&A on Friday night. The video will begin above at 8:30 p.m. ET. read news >>
Prior to the UFC Fight Night 65 weigh-in on Friday in Adelaide, Australia, Alistair Overeem, Jessica Eye and Michael Chiesa will field questions from the fans down under. read news >>
Three-for-one special! Ultimate Fighting Championship (UFC) will host a special fighter Q&A later this evening (Fri., May 8, 2015) at 7:30 p.m. ET to kick off the UFC Fight Night 65 weigh ins inside read news >>
Episode No. 6 of The MMA Road Show with John Morgan podcast, featuring Tom Wright, Alistair Overeem, Jessica Eye and Michael Chiesa, is now available for streaming and download.Filed under: News, UFC read news >>
"Cigano" also says "The Reem" is a much better talker than he is fighter. Alistair Overeem has made it a point to prove to mixed martial arts (MMA) fans -- and Ultimate Fighting Championship (UFC) Pr read news >>
Injured Junior dos Santos responded to Alistair Overeem's recent comments about wanting to fight him. read news >>
Alistair Overeem was matched up twice against Junior dos Santos in the UFC, but had to pull out both times for different reasons. "The Reem" is once again calling out the former champion, and the Bra read news >>
Check out the Mayweather vs. Pacquiao picks from UFC and combat sports pros including Rumble Johnson, Alistair Overeem, Renzo Gracie, and many more. It's Thursday, April 30, and with 2 days away, the read news >>
Check out BloodyElbow.com's photos from Alistair Overeem's recent workout in Manila read news >>
In part two of Alistair Overeem's interview with BloodyElbow.com, he spoke about rooting for Conor McGregor, Manny Pacquiao vs. Floyd Mayweather, the Reebok deal, and much more. In the second part of read news >>


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"Thanks to the support of Coca-Cola, this promotion marks the first time we've had anywhere near 35,000 7-Eleven stores participating in one event at the same time," says executive vice president of 7-Eleven. Big things happen when you combine the world's most iconic brand and the world's largest convenience retailer with the most widely viewed
7-Eleven and Coca-Cola Celebrate 2014 FIFA World Cup
Grab & Go Hummus and Multi-grain Pita Chip Packs
Kronos Foods Inc., a manufacturer of Mediterranean food products, has announced the introduction of its Grab & Go Hummus and Multi-grain Pita Chip Packs for convenience stores and foodservice locations with on-the-go options nationwide. Available in classic and chipotle flavors, the Mediterranean-inspired hummus combines nine grams of protein and six grams of fiber per serving
Wawa Celebrates Hoagie Day
Features five-ton Hoagie Salute and 10,000 pounds of Hoagie goodness. As part of its fifth year as title sponsor of Wawa Welcome America!, Wawa will be hosting its most patriotic and meaningful Hoagie Day to date featuring the building and serving of a five-ton hoagie. Wawa's 2014 Hoagie Day celebration marks the 22nd year since
Smoker Friendly Acquires Havana Manor Cigar Shop and Club
Venue expected to be a great addition for cigar events. The Cigarette Store Corp. and Smoker Friendly have purchased Longmont, Colo.-based Havana Manor Cigar Shop and Club. Mike Landau, founder of Havana Manor Cigar Shop and Club, made the decision to sell to the Gallagher Family, who have been long-time friends. He plans to step
Enmark Raises $2,000 for Girls on the Run of Coastal Georgia
Funds to be used for scholarship opportunities. Matching proceeds raised from Paula Kreissler's "Five Strategies for Busy Families to Stay Healthy," the second of five lectures in the Enmark Encourage Health Education Series 2014, Enmark Stations raised $2000 for Girls on the Run of Coastal Georgia, a local Savannah nonprofit. The nonprofit raised an additional
Eby-Brown Welcomes New Vice President of Strategic Insights
Jim Hachtel, with 32 years of convenience retailing experience, joins Eby-Brown. Eby-Brown Co. LLC welcomes former BP/ ampm Senior Category Manager, Jim Hachtel, as vice president of strategic insights. In this role, Hachtel will focus on Eby-Brown's SmartProcess. "I've always been passionate about the convenience channel of trade, and helping our great retailers grow their
Service Information For MacDougall Announced
Services have been announced to celebrate the life of Nice N Easy Grocery Shoppes Founder John MacDougall, who passed away recently at the age of 73. The following services for John's Celebration of Life have been arranged for Friday, July 25: Mass at 10 a.m. (limited room) Immaculate Conception Church 400 Salt Springs Street Fayetteville,
Data Capture Solutions Recommends CipherLab For Convenient Store Ordering
Let's put the word "Convenient" back into owning a convenient store! With technology at your finger-tips that's also easy on your budget Data Capture Solutions can change the way you do business. Since 1992 Data Capture Solutions has provided innovative solutions, bar code scanners, handheld devices, wireless technologies, software and services to more than 1,500
Minimum Wage Trade Off
Tony Huppert, CEO, Team Oil Inc. I have said over and over, politicians' raising the minimum wage to gain votes is now coming to haunt those responsible. Headlines are reading "The New Economy: Unemployment rate falls, but teens get left behind." As economists will insist, raising the minimum wage doesn't affect the numbers substantially. It
Studies Show E-Cigarettes Help Smokers Quit
While detractors may declare otherwise, science is showing that e-cigs are a safer alternative to traditional tobacco products. By Gregory Conley, Research Fellow, Heartland Institute For years, advocates for smoke-free alternatives, such as electronic cigarettes and other e-vapor products, have known that these products are effective at helping smokers quit or dramatically reduce their cigarette
Crime Prevention 101
Clear visuals, strict till limits and great customer service are keys to deterring convenience store robberies. Marilyn Odesser-Torpey, Associate Editor Robbery occurs less often than some other crimes, but the potential for loss, injury and death is much greater during a single robbery incident, according to the convenience store security guidelines for owners and managers
Why Customers Choose E-Cigarettes
Electronic cigarette sales are surging. CSD partnered with Study Hall Research to go inside the minds of e-cigarette consumers to learn what motivates their tobacco purchases. By John Lofstock, Editor and Josh Tahan, Study Hall Research The prevalence of electronic cigarette usage amongst the general population is growing and analysts are predicting the category now
Meeting the Challenge
Aggressive pricing and eliminating product outages are two keys to maintaining cigarette sales. By Howard Riell, Associate Editor Even as the buzz of e-cigarettes continues to grow, traditional cigarettes remain an essential product for the convenience store industry. However with increasing pressures from government and regulators, cigarette sales continue to decline with seemingly no let
E-Cigarette Sales Are Smoking
Despite proposed FDA rules and looming tax hikes, e-cigarettes remain a breath of fresh air for c-store sales. By David Bennett, Senior Editor While the debate on electronic cigarettes is heating up among state and federal officials, convenience stores are pressing forward with broader product offerings, driving e-cigarette sales ever higher. Just ask Mike Gancedo,
Parsing Data, Events to Understand Trend in U.S. Gasoline Demand
By Brian Milne, Energy Editor for Schneider Electric. The gasoline futures market on the New York Mercantile Exchange dropped back a nickel from a fresh 11-month high of $3.1520 gallon reached June 23, but remain over $3 gallon while the U.S. retail price average at $3.704 gallon will continue to advance into July as pass
FDA Offers Training Video On Tobacco Sales
Video to help retailers with vending machines or self-service displays comply with laws. FDA has released a new compliance training video for tobacco retailers pertaining to impersonal modes of tobacco sales, such as vending machines or self-service displays. The video explains the restrictions associated with impersonal modes of sale of regulated tobacco products and will
Amazon Takes On Takeout Food Delivery
Restaurant food is set to be available through Amazon in select markets. Amazon is rolling out a food takeout service, a direct competitor to GrubHub, Seamless and DeliveryHero, Tech Crunch reported. The service initially will be part of Amazon Local, the company's Groupon-style service and app that offers people daily deals, coupons and discounts from
Ari Haseotes Named CEO of Cumberland Gulf Group
Haseotes takes helm of multi-billion dollar company established 75 years ago by his grandparents. The Cumberland Gulf Group, which includes Cumberland Farms, the highly successful convenience store chain, and Gulf Oil L.P., one of the nation's fastest growing branded marketers of energy products, announced that Ari Haseotes has been named CEO. Haseotes, grandson of founders
Paychex Brings On-the-Go Accounting To Small Businesses
Launches Accounting Online iPhone App. Paychex Inc., a provider of payroll, human resource, insurance, and benefits outsourcing solutions for small- to medium-sized businesses, announced the launch of its Paychex Accounting Online mobile application for iPhone. The Universal iOS app allows users to access their Paychex Accounting Online account from their iPad, iPhone, and iPod Touch
Roller Grill Still Real
Made-to-order may be the current darling of a growing number of c-stores, but a well-run roller grill program is still central to successful fresh foodservice. By Marilyn Odesser-Torpey, Associate Editor They smell it. They see it. They want it. For most convenience stores, the roller grill is the customers' first introduction to the store's fresh
Parker's Drive-Through Open For Business
New Statesboro, Ga. store opens and in celebration, Parker's offers a 10 cents per gallon discount on all fuel types across Statesboro. Parker's new store in Statesboro, Ga. at 1588 Brampton Ave. opened, June 25, marking the company's 36th store in the Georgia and South Carolina area. The modern convenience and fuel store features Parker's
QuikTrip Opens 700th C-Store
Plans to roll out QT Kitchens to most stores across the country. Tulsa, Okla.-based QuikTrip Corp. opened its 700th store on Thursday, June 26, in St. Louis, the Tulsa World reported. The milestone marks the fastest expansion in the history of the convenience chain, which has opened 100 stores in the last two years alone.
Murphy USA Opens E15/E85 Station
Plans to offer E15/E85 at seven stations by summer's end. Murphy USA is now offering E15 and E85 at its Indianola, Iowa location. The location marks the first of seven Murphy USA locations in Iowa set to begin offering higher ethanol blends. By the end of summer, Murphy USA plans to roll out E15 and
BP Brings Olympic and Paralympic Spirit to Chicago
Sponsors ITU World Triathlon Race. BP is a main sponsor and the official energy partner of this weekend's International Triathlon Union (ITU) World Triathlon Chicago, furthering its long-running association with U.S. Olympic and Paralympic sports. "As a proud partner of the United States Olympic Committee, BP is honored to sponsor ITU World Triathlon Chicago, where
Jiffy Mart Wins Business Breakthrough Award
Jiffy Mart was recently honored by the Maryland Chamber of Commerce for a Business Breakthrough Award. For the second year in a row, multiple Carroll County, Md. businesses were nominated for and won the Business Breakthrough Award, sponsored by the Maryland Chamber of Commerce. Convenience store chain Jiffy Mart and Tevis Motor Fuel won in


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Pickens' plan: run heavy trucks with natural gas
Tom Jackson
| March 10, 2009 |
Regardless of what you think about oilman T. Boone Pickens, he's spot on when he says we should run our heavy diesel trucks on compressed natural gas. The only drawback to such a plan is you need to build an infrastructure to do so, but that couldn't be any more challenging than the infrastructure we're soon going to have to develop for SCR urea supplies.
After that, CNG is all gravy: much less pollution, lower carbon footprint and best of all for the economy, the money stays in country. The United States, as some have said, is the Saudia Arabia of natural gas.
From our partners
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More From: Construction News


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Biotech stocks are absolutely on fire
You have to take risks in order to succeed
Join the NASDAQ Community today and get free, instant access to portfolios, stock ratings, real-time alerts, and more!
LSI Industries Inc. (
) will begin trading ex-dividend on August 29, 2013. A cash
dividend payment of $0.06 per share is scheduled to be paid on
September 10, 2013. Shareholders who purchased LYTS stock prior to
the ex-dividend date are eligible for the cash dividend payment. At
the current stock price of $6.95, the dividend yield is 3.45%.
The previous trading day's last sale of LYTS was $6.95,
representing a -20.11% decrease from the 52 week high of $8.70 and
a 13.93% increase over the 52 week low of $6.10.
LYTS is a part of the Consumer Durables sector, which includes
companies such as Acuity Brands Inc (
) and AZZ Incorporated (
For more information on the declaration, record and payment
dates, visit the
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These boards feature a pulled in nose and a little extra rocker to give you that surfboard feeling but still maintaing good glide.
The back of the boards feature a pulled-in outline with increased V. Once on the wave face, you just shift your weight slightly back and you will experience fast rail to rail response and agility resulting in tighter turns while maintaining speed. This gives the advanced SUP surfer some vertical turns, a feature rarely found in SUP surf boards.
The whole surf range comes with a FCS plug in the nose to attach your Go Pro camera. All SURF boards come with a Thruster setup. They also come with a mast foot insert to attach a windsurf rig and are great lightwind wave boards.
Construction Technology
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8'6″ x 29″
The level of SUP Riders in the waves pushes for more radical shapes. We extended the Surf Line with an additional more radical size. Not just shorter but also only 29″ wide. The perfect toy for the advanced wave ripper who is looking for a board that has no limits.
8'10″ x 30″
This is a proven shape, new graphics and constructions. This is a shop favorite!
For the advanced SUP surfer who likes to go big. Ultimate control and snappy turns will push your wave riding to higher grounds.
9'2″ x 30″
This new shape replaces the 9'3″ and comes with the same, more radical outline like the rest of the surf line. The more pulled in nose does not limit the rider in radical bottom turns. In combination with the fast scoop rocker line the board develops lots of drive on the wave face. Thin smooth rails slice effortless through the water surface and provide incredible grip. Like the rest of the line this shape features a concave nose section running into a flat V which increases towards the tail. This in combination with the accelerating tail kick gives the board an incredible loose ride and makes it a very versatile wave toy.
9'6″ x 31″
Another proven shape, new graphics and constructions.
A slightly wider and longer version of the 9'2″. Bigger, advanced SUP surfers who are looking for that short board feeling will love it. Wide in the middle for stability combined with a narrow tail, guarantees maximum control and lots of fun for big guys in big surf.
The Surf line is more aimed at bigger waves while the Surf Wide Bodies work great in smaller waves and beach breaks. Depending on the size both concepts work for intermediates to experts.
Additional Information
|JP Australia
|Free Shipping


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Conn Brown may be delayed
Aransas Pass, state can't agree on land appraisal values
ARANSAS PASS - Development of Conn Brown Harbor hinges on a land swap between the city and the state, but the parcels of land appear to be of significantly different values. That difference may slow development.
The city voted in February to allow Villanueva Investment Group Inc. to develop the harbor after the company presented a $250-million plan for a marina, a luxury hotel, restaurants, convention center and condos. Any development hinges on the city owning all the harbor land, which they would then sell or lease to VIG.
While neither the city nor the state's General Land Office will reveal the appraisal values of the parcels of land in question, citing ongoing negotiations, both indicated a difference in values with the state's land much higher.
In July 2005, when legislation passed allowing for the swap, early estimates from the land office put the value of its land at $4 million and the city's land at $578,000. "The disparity in property values is the subject of the negotiation," said City Manager Scott Thompson.
Thompson would not comment about the negotiations or whether the city would add more property to its offer or supplement it with cash.
"What is the bottom line? That's what we're trying to figure out," he said. "When it's all said and done, we're going to come to the public and say this is the situation."
He said recently reported figures putting the city land at nearly $1 million and the state's land at about $15 million represent a starting place.
"But things are continuously changing," Thompson said.
City officials said both pieces of land are about 90 acres. The land office property is in the middle of the harbor, and the city's land is farther south on the Intracoastal Waterway near Ingleside in the Aransas Pass extra-territorial jurisdiction. But the General Land Office map puts the city's trade land at 65 acres and lists its land as 53 acres in the harbor (and 14 acres submerged); and 27 in the LWR property; and six at the airstrip.
"The land office and the city both agree that if that area is developed everybody will be able to make money," said Jim Suydam, spokesman with the land office.
But the state isn't looking to give land to the city or lose money in the swap.
"The land office is all about making money for the permanent school fund," Suydam said.
The residents of Aransas Pass have been at this point before. A $20 million plan for restaurants, boat slips and retail shops was approved in May 2004, but dropped seven months later in December. Council members said then negotiations regarding a section of state-owned land in the harbor, among other things, stalled the project.
Ongoing negotiations now indicate the project is still in the works, but neither side will say if this delays the development timeline.
The two sides continue to meet weekly, and Thompson said all sides are working together for a positive outcome, development of the harbor.
The first phase of development is scheduled for completion by spring 2008.
Thompson said all options, including a long-term lease by the city from the land office, would be considered.
Contact Beth Wilson at 886-3748
Subscribe to the Caller-Times
Need Help? Call us at 1-800-566-8098.
Monday-Friday: 5am-8pm / Saturday: 7am-12pm / Sunday: 6am-1pm


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Sonny Bill Williams to play for the Roosters in 2014 and has rejected a return to rugby union
- From: The Daily Telegraph
- October 11, 2013
SYDNEY Roosters have won the battle for the services of code-hopping superstar Sonny Bill Williams in 2014.
News broke when the official All Blacks twitter account tweeted that he had rejected the offer to return to play for the Chiefs in the 2014 Super Rugby competition.
The Sydney Roosters shortly after sent out a statement that Williams had agreed to remain with the premiers for next season. Sources close to the Williams camp said it still remained his dream to return to rugby union and play in the 2015 World Cup.
We are very pleased to OFFICIALLY announce that @SonnyBWilliams has pledged his commitment to the Roosters for 2014 - http://t.co/U5n1pXxsqt— Sydney Roosters (@sydneyroosters) October 11, 2013
"On the eve of his departure for the Rugby League World Cup, the Sydney Roosters are pleased to announce that Sonny Bill Williams has pledged his commitment to the Roosters for season 2014," the statement said.
Have your say
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Comments on this story
Joel Posted at 3:15 PM October 11, 2013
BOOOOYAAAA! back to back baby!!!!
Comment 1 of 20
Rooster Booster of Wild Wild West Posted at 3:19 PM October 11, 2013
Great News for every Rooster fan and the whole Rugby League world. This is like when Michael Jordan came back to the Chicago Bulls and they went on to win 3 more NBA titles. A dynasty is born!!!
Comment 2 of 20
Wllhamm of Darwin Posted at 3:25 PM October 11, 2013
Yeah right. Until his signature is on that contract in February nobody will believe a word of it.
Comment 3 of 20
GP of Brisbane Posted at 3:33 PM October 11, 2013
Oh how forgetful we are when sports villains are in demand. This is the 'walk-out King'......even if the chooks at the Roosters had a signature on a contract, SBW will do what SBW wants, when he wants and to whom he wants. A man's word is worthless if not backed up by actions!
Comment 4 of 20
Mal Posted at 3:41 PM October 11, 2013
It's good to see that he is staying with the Roosters.
Comment 5 of 20
Jacques de Pom Posted at 3:45 PM October 11, 2013
Nothing to do about the game ...all about the $$$$ in his pocket . He is totally overpaid and over rated . Its all about massaging SBW's ego and marketing the SWB brand . He is as only as good as the players around him and his coaches; he didnt stop because of the "adoring fans" , no , it was the money. He and his "advisors" just played NRL off against NZRU ; he just wanted more money , Dont be fooled by the rhetoric .Wise up , Roosters you have 10 other players on your books who could have done what he did last Sunday all you are getting is a bulked up narcistic money grabber who fits the wider PR image for the NRL , its sponsors and enhanced television rights . What has our game become ?
Comment 6 of 20
Nick Posted at 3:49 PM October 11, 2013
even as a rooster supporter I would have to be worried about the salary cap implications of this deal
Comment 7 of 20
Casper of Nirvana Posted at 3:50 PM October 11, 2013
So what, anybody would think this bloke is god's gift to the game. It only matters to the Roosters and the media. He didn't rank in the Dally Ms yet the media would want you to believe he's something out of the ordinary. Does this mean we're going to get this media love child rammed down our throats again? I HOPE NOT!
Comment 8 of 20
chris Posted at 3:51 PM October 11, 2013
i hope schubert is watching for the 'old brown paper bags' trick. no way they keep this side together after winning the GF. look at Manly who have had to shed players the last 5 years and the roosters keeping this side intact?? seriously
Comment 9 of 20
Jonathan Posted at 3:52 PM October 11, 2013
its amazing the power uncle nick has over at the nrl
Comment 10 of 20
Dave of Canberra Posted at 4:13 PM October 11, 2013
Back-to-back baby!
Comment 11 of 20
Same rules for all? of Newcastle Posted at 4:16 PM October 11, 2013
No doubt Mr Schubert will scrutinise this contract as closely as he does all Roosters contracts - i.e. the tick and flick. Amazed that he won't sign until Feb 2014 - lots of scope for payments outside the salary cap....
Comment 12 of 20
Robert of Mooloolaba Posted at 4:20 PM October 11, 2013
Ooh! Rugby can have him, now the NRL is stuck with him for another year. I'm over him.
Comment 13 of 20
Brett of Perth Posted at 4:24 PM October 11, 2013
Very surprised to hear he'll be back in the NRL next year! I thought it was a 1yr deal and he'd be out of here for sure, but good to hear he'll play on. No doubt next year will be his last, at least until after the RU world cup, but that's ok I get it. Although I'm not a Roosters fan, it's good for the game. About time we had some good news in the NRL.
Comment 14 of 20
Walter the sane of Wattle Grove Posted at 4:34 PM October 11, 2013
Once the Tricolours get in your blood, the pride and passion is hard to walk away from! What a sad disappointment it would be for SBW to go from this year's glory to Rugby YAWNion... Bring on 2014, Go you mighty Roosters!!
Comment 15 of 20
Hokey Pokey McDokey of Toowokey Posted at 4:36 PM October 11, 2013
Fantastic News. The impact he has had on his team mates this year with his level of professionalism will only be magnified with another 12 months. The roosters will set a new bench-mark for the other NRL teams to follow in teams of what is required from professional footballers. A season free of binge drinking and commitment to doing what it takes to win a premiership. A couple of teams will follow and the rest will get the drift soon enough. Then we will see each game in the NRL lift to the standard of last weekends GF.
Comment 16 of 20
RG of Qld Posted at 4:54 PM October 11, 2013
Stay in League. Union is a truly a world game and there are a host of stars around the world in the game. League needs to keep all the "stars" it can.
Comment 17 of 20
Kevin Ross of Sydney Posted at 5:05 PM October 11, 2013
Thank god he's not coming back to rugby union. Overrated.
Comment 18 of 20
nim Posted at 5:06 PM October 11, 2013
what is he looking up in the sky??? money falling
Comment 19 of 20
Rossco of Sydney Posted at 5:27 PM October 11, 2013
I'm a fan of both codes and he's great in both, but prob best to stay in League in my opinion - the AB's have a queue of people who can replace him and he is better suited to League and probably suits his profile more. What else can you say about him - he has the world at his feet, no bloke could blame the guy for being in a state of indecision ! Good luck to him
Comment 20 of 20


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Which rivalry is greater? Fedal or Djokodal?
View Single Post
Join Date: May 2011
Posts: 9,342
Originally Posted by
Pretty much. As far as Fed is concerned:
He has won 33% of all the matches he has played with Rafa(10-20).
He has won 55% of all the matches he has played with Djoko(16-13).
He has won 45% of all the matches he has played with Murray(9-11).
I like all combinations of the top 4 except Djoko-Murray. Nadal has his lefty factor and Fed has wicked versatility so you know that they bring something special to the table whereas Djoko and Andy have games that are all too similar.
As for which rivalry is greater, I still say Fedal because of historical importance. Djokodal is starting to gain ground though:
Fedal have met 10 times in slams, 8 times in finals.
Djokodal have met 10 times in slams, 5 times in finals.
Fedal have met 15 times in MS, 10 of those being finals.
Djokodal have met 20 times in MS, 10 of those being finals.
yeah Murray vs Djokovic is pretty boring, god help us because that's looking the next rivalry...
really weird to think that Djokovic and Federer have met in slams more than Djokodal or Fedal, yet only one final compared to 5 and 8
How to clarky - work out most or only realistic outcome, claim the opposite
Federer, Djokovic, Delpo fan (also like Nalbandian, Dimitrov, Tsonga)
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Just 400 metres from Cape Town International Airport and a 15-minute drive to the city's main attractions, Hotel Verde offers you a carbon-neutral stay, along with luxurious accommodation and conferencing facilities.
Cape Town Guide
Hotel Verde welcomes all our guests to Cape Town!
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Concise Analysis of the International and Chinese Ion-Exchange Resin Industry
DUBLIN, Mar. 24, 2014 /PRNewswire/ –
Research and Markets
) has announced the addition of the
"Concise Analysis of the International and Chinese Ion-Exchange Resin Industry"
report to their offering.
(Logo: http://photos.prnewswire.com/prnh/20130307/600769)
Concise Analysis of the International and Chinese Ion-Exchange Resin Industry is a professional and in-depth research report on the global ion-exchange resin industry with a focus on the Chinese situation and data forecast for the period 2013-2018. The report first provides basic information on the ion-exchange resin industry including definitions, classification, applications, industry chain structure and industry overview. A comparison is carried out between the international and Chinese domestic market. A production technology overview is also provided covering sections such as production line, origin, R&D analysis, equipment and raw materials.
Ion-exchange resin industry policy, news and development trends are analyzed and the manufacturing process and cost structure delineated. Productions, supply, sales, demand, market status and forecast analysis is offered for the period 2009-2014 providing comprehensive data.
Key Topics Covered:
Chapter One Ion-exchange Resin Overview
Chapter Two Ion-exchange Resin China Market Analysis
Chapter Three Ion-exchange Resin Production Technology Overview
Chapter Four Ion-exchange Resin Policy and Industry News
Chapter Five Ion-exchange Resin Manufacturing Process and Cost Structure
Chapter Six 2009-2014 Global and China Ion-exchange Resin Productions Supply Sales Demand Market Status and Forecast Analysis
Chapter Seven Global and China Ion-exchange Resin Key Manufacturers Analysis
Chapter Eight 2009-2014 Global and China Cation Anion Ion-exchange Resin Productions Supply Sales Demand Market Status and Forecast Analysis
Chapter Nine Up and Down Stream Industry Analysis and Influence
Chapter Ten Ion-exchange Resin Marketing Channels Analysis
Chapter Eleven 2014-2018 Ion-exchange Resin Industry Development Trend
Chapter Eleven Ion-exchange Resin New Project Investment Feasibility Analysis
Chapter Thirteen Ion-exchange Resin Industry Research Conclusions
Companies Mentioned:
-- DONGYANG MINGZHU -- Dow Chemical -- Finex Oy -- Hebi Juxing -- Jiangsu Linhai Resin -- Jiangsu Success -- KaiRui Chemical -- Langfang Shengquan -- Lanxess -- Mitsubishi Chemical -- Pure Resin -- Purolite -- ResinTech -- SUNRESIN -- Samyang -- Sanxing Resin -- Shanghai Huazhen -- Shanghai Resin -- Suqing Group (former Jiangyin Organic Chemical Plant) -- Suzhou Bojie -- THE CHEMICAL PLANT OF NANKAI UNIVERSITY -- TIANGANG -- WANDONG -- XIAN DIANLI -- Zhejiang Zhengguang -- Zhengda Lanxing -- Zibo Dongda Chem
For more information visit http://www.researchandmarkets.com/research/mdzsm9/global_and
Media Contact:
Laura Wood , +353-1-481-1716, email@example.com
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Sheridan Creek Equestrian Ctr
50 - 100 downloadsAdd this app to your listsTweets por @Appszoom
Sheridan Creek Equestrian Center is an exceptional full service boarding, teaching and learning facility owned and directed by Kristen Roberson. Our goal at Sheridan Creek Equestrian Center is to provide exceptional quality care for our equine partners. With our mobile app you can schedule lessons, sign up for learning clinics, horse shows and summer camps and much more...
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SALT LAKE CITY (AP) — Mo Williams had just missed from 3-point range with 9.9 seconds left and the game tied against Western Conference nemesis San Antonio.
Williams' 3 at buzzer lifts Jazz over Spurs 99-96
Instead of getting grief from his coach, Williams got another green light.
"Coach told me don't worry about it. You'll make the next one," Williams said.
He did.
Williams hit a 3-pointer at the buzzer to lift the Utah Jazz to a 99-96 victory Wednesday night that snapped the Spurs' five-game winning streak.
"It showed how much my teammates believe in me, showed how much the coaches believe in me," Williams said.
Now Utah players believe in their team, having knocked off the Lakers in Los Angeles on Sunday and a Spurs team that swept them out of the playoffs last season. It was the first time since 1997 that the Jazz beat both in back-to-back games.
"We're on a mission," Williams said. "We want to be good. We don't know our ceiling yet."
Paul Millsap led Utah with 24 points and 12 rebounds, Al Jefferson scored 21 and reserve Gordon Hayward added 19.
The Jazz trailed by eight with a little more than 4 minutes remaining. Williams' winning shot was his only 3-pointer of the night and he finished with eight points on 3-for-9 shooting.
Spurs coach Gregg Popovich said Williams was able to get off the final shot because of a "huge" defensive error by Danny Green when the Jazz inbounded the ball with 6.7 seconds left.
"You got to make him drive with that amount of time," Popovich said. "That's why we put a bigger guy on Mo at the end so he couldn't get a shot off. You don't step off Mo Williams. What do you think he's going to do with two seconds on the clock? Instead of getting into him, he backed off. Huge defensive error."
After draining the shot, Williams looked up to make sure there was no time left then took off running toward the other end of the court only to be mobbed by teammates.
"It was amazing," said Millsap, who grabbed the key offensive rebound after Williams' first miss. "It was a big win for us, a big win for our fans, a big win for our whole organization."
Tim Duncan and Tony Parker each scored 22 points to lead the Spurs, who swept the Jazz out of the playoffs in the first round and had won 11 of the last 12 meetings.
Green's 19-footer gave the Spurs a 96-94 lead and Millsap tied it with 40 seconds left.
While Duncan and Parker have been key for the Spurs, who entered Wednesday with an 11-2 road record, neither could make the shot at the end. Both missed long outside shots in the final 75 seconds as the Jazz improved to 9-1 at home.
"It was disappointing down the stretch," said Duncan, who also had 21 rebounds and six blocks. "Individually I made too many mistakes. I missed a jumper on the baseline I thought was down. Al (Jefferson) made a great play on the steal and layup. Just too many mistakes down the stretch that we couldn't recover from."
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text: BooknewsContains graphical methods and principles for visualizing data in science and technology. Emphasizes the basic ideas, methods, and principles for creating readable graphs through an understanding of human visual and graphical perception, with a small section on computer graphics. Covers graphical methods, such as logarithms, visual reference grids, and statistical variation, and issues in graphical perception, including superposed curves, color encoding, and graphing along a common scale. Annotation c. Book News, Inc., Portland, OR (booknews.com)


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BERWICK — Not only did Joe Zielinski build the foundry that turned out more than 15,000 Stuart tanks during WW II, he built the tanks too.
The 90-year-old former welder at the American Car and Foundry Co. turned out Sunday to see his work as a 1942 model came to town by way of Oregon, where it's part of a private collection of military vehicles.
The tank drove in the Columbia County bi-centennial parade in Bloomsburg on Saturday and was on display at the Test Track Riverfront Park in Berwick, where the vehicles were driven before being shipped out for use in battle.
Zielinski, of Nescopeck Township, tapped a wooden walking stick against the track assembly of the drab-green tank with serial number 10103. He used to weld parts of the assembly and pulled a July 18, 1942 ACF pay stub from his pocket.
He made nearly $106.95 for a 40-hour work week back then.
"That's more money than my two brothers were bringing home at the same time," Zielinski said.
They were in the service and he was at home working. As the last boy on the farm he wasn't drafted and the farm was overproducing, as well.
He finished high school and was working construction on the foundry. "I think I was making 28 cents on hour," he said.
When a welding school started up at night at Nescopeck High School, he attended, and after five or six sessions was ready for work at the foundry.
"The instructor said, 'Tomorrow you report to the ACF,' " Zielinski said.
He spent nearly two years welding axles and wheels and found a mark to show he worked on the tank.
"When you made a wheel you had a stencil," he said, pointing his stick to a mark on the side of a steel box above one set of tracks.
Zielinski would have been the type of employee Frederick Shepperly could have used. The 91-year-old former Marine Corps captain from Berwick who was in the Pacific Theater during WWII ended up in the service and supply department for the First Division.
"Our object was to salvage tanks after each island invasion. I enlisted almost 100 guys from Berwick, Pennsylvania, who worked at ACF and had talents as spare parts people, as mechanics, electricians and they went overseas with me," Shepperly said.
Approximately 500 tanks were issued to the divisions and between 200 and 300 were left after the invasions.
"We repaired what we could and the rest went to salvage," Shepperly said.
The last time he was this close to one of the tanks was 70 years ago.
Like many of the people who came to see a piece of local history, Shepperly longed for having one on display in its hometown.
"My greatest ambition would be if they could get one of these tanks for Berwick," he said.
Columbia County Commissioner David Kovach said he and others are working on it.
"We've been looking since 2004. We've found several. They want $200,000 for all the ones that we've found," Kovach said.
He thanked Steve Greenberg, the owner of the tank, for making it available and bringing back so many memories.
The tanks are available, and one sold recently in Ohio for $180,000, said Greenberg, 55, of Wilsonville, Ore.
The collector said he was amazed at the appreciation expressed by the crowds at the parade and the display.
Greenberg said he especially happy for Shepperly.
"It's just so neat that he had an opportunity to be in this tank 70 years later," Greenberg said.
Before loading it on a flatbed trailer for a return to the Pacific Northwest, Greenberg drove the tank around the test track and fired three blank rounds from its 37 mm gun. A bugler sounded taps afterward.


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Actinobacillus pleuropneumoniae is an economically important animal pathogen that causes contagious pleuropneumonia in pigs. Currently, the molecular evolutionary trajectories for this pathogenic bacterium remain to require a better elucidation under the help of comparative genomics data. For this reason, we employed a comparative phylogenomic approach to obtain a comprehensive understanding of roles of natural selective pressure and homologous recombination during adaptation of this pathogen to its swine host.
In this study, 12 A. pleuropneumoniae genomes were used to carry out a phylogenomic analyses. We identified 1,587 orthologous core genes as an initial data set for the estimation of genetic recombination and positive selection. Based on the analyses of four recombination tests, 23% of the core genome of A. pleuropneumoniae showed strong signals for intragenic homologous recombination. Furthermore, the selection analyses indicated that 57 genes were undergoing significant positive selection. Extensive function properties underlying these positively selected genes demonstrated that genes coding for products relevant to bacterial surface structures and pathogenesis are prone to natural selective pressure, presumably due to their potential roles in the avoidance of the porcine immune system.
Overall, substantial genetic evidence was shown to indicate that recombination and positive selection indeed play a crucial role in the adaptive evolution of A. pleuropneumoniae. The genome-wide profile of positively selected genes and/or amino acid residues will provide valuable targets for further research into the mechanisms of immune evasion and host-pathogen interactions for this serious swine pathogen.
In the evolutionary history of many microorganisms, positive selection and homologous recombination are two indispensable driving forces for adaptation to new niches. Both of them contribute to the genetic variations that might influence the population diversification and adaptation of pathogenic microorganisms [1,2]. Recent studies on the genome-wide evolutionary dynamics have highlighted the important roles of selection and recombination in the molecular evolution of bacterial pathogens, including Escherichia coli [1], Listeria monocytogenes [3], Salmonella spp. [4], Streptococcus spp. [5], and Campylobacter spp. [6]. These analyses have revealed that a certain number of protein-coding genes subject to natural selection pressure are usually involved in the dynamical interactions between host and pathogen, especially in the immune and defense-associated functions [1]. Diversifying selection operating on these genes may be caused by pathogen-host co-evolutionary arms race [7,8].
In the present study, dN/dS-based methods were applied to detect evidence of genome-wide positive Darwinian selection. Estimating the ratio (ω) of the rate of nonsynonymous nucleotide substitutions (dN) to that of synonymous substitutions (dS) is a powerful approach for measuring selective pressure on the protein-coding level: ω = 1, < 1, > 1 indicate neutral evolution, purifying (negative) selection, and positive (adaptive) selection, respectively [9,10]. The codon models further developed by Nielsen and Yang allow variation in ω among sites [11], which have an extensive capability to find evidence for adaptive evolution in most functional genes where only a small fraction of amino acid sites are subject to strong positive selective pressure [12]. Thus far this approach has been widely used for genome-wide selection analyses in pathogenic viruses, bacteria, and eukaryotes [9,13]. A substantial number of genes encoding highly variable antigens are identified to undergo adaptive selection particularly on some functional sites for evasion of host immunity [1,4,14].
Actinobacillus pleuropneumoniae, a Gram-negative coccobacillus belonging to the Actinobacillus genus of Pasteurellaceae family, is a strictly swine pathogen and colonizes in the upper respiratory tract of porcine [15]. This pathogen has caused an economically severe disease characterized by pulmonary lesions, pleuritis, and pericarditis in pigs [16]. According to the differences in capsular polysaccharides, A. pleuropneumoniae has been divided into 15 serovars [17]. The recent comparative genomics studies through both high-throughput approaches of genome sequencing and microarray have depicted the compositions of the pan-genome and confirmed the contribution of genes loss or gain to the diversity in virulence and serovar of A. pleuropneumoniae [18,19]. However, besides large genetic variations resulting from DNA acquisition and genome reduction, small sequence differences occurring in the conserved genes, including point mutations, insertions/deletions (indels), and intragenic recombination, may also play a crucial part in the alteration of antibiotic resistance, pathogenicity and immunogenicity [20,21]. But to date, no research pays enough attention to the linkages between genetic alterations and putative functional roles in intraspecies conserved genes of A. pleuropneumoniae at the whole genome level.
In order to further trace evolutionary trajectories on the core genome of pathogenic bacterium A. pleuropneumoniae, we employed a genome-wide analyses approach to investigate the effects of natural selection and homologous recombination operating on the coding genes. Our analyses focused on the evolutionary characterizations of core genome genes that are shared by 12 A. pleuropneumoniae genomes. Many genes were shown to be under strong positive selective pressure and primarily associated with the fitness and immunogenic properties of this swine pathogen.
Genome dataset and alignment
Twelve genome sequences of A. pleuropneumoniae were retrieved from NCBI Genome database (http://www.ncbi.nlm.nih.gov/genome webcite). The sequences included 3 complete genomes and 9 draft genome assemblies (see details in Table 1). Orthologous gene content information and annotation with COG functional classification have been defined in our recent work and used here [19]. To increase accuracy and power of selection analyses, an ortholog set was excluded if it satisfied any of the following criteria: the length of any gene lower than 80% of the maximum length, more than one gene from each genome or less than four sequences. Protein-coding sequences longer than 50 codons were used in this study. Subsequently, the orthologous protein sequences were aligned using a progressive method implemented in T-Coffee v8.93 [22].
Table 1. Genome sequences of A. pleuropneumoniae used in this study
Frameshift mutations (indels of a number of nucleotides not divisible by three) can lead to high nonsynonymous substitution rates, resulting in more false positive results when positive selection was estimated based on dN/dS ratios [5]. To avoid incorrect indels in the codon alignments, multiple sequence alignments were initially performed with amino acid sequences from each gene cluster, followed by conversion to the corresponding codon alignments using custom Perl scripts. The coding sequences located at the beginning or end of the contigs appeared to be more prone to frameshift sequencing errors. Therefore, we further assessed the quality for each alignment through obtaining the following information: overall identity, and identity in the first 30 nt and last 30 nt per alignment. The codon alignment sequences that contain frameshift mutations were checked and edited manually in the software MEGA4 [23] if identity is low.
Calculation of dN, dS, codon bias, nucleotide diversity and informative sites
According to the method as defined by Nei and Gojobori [24], the number of synonymous nucleotide substitutions per synonymous site (dS) and the number of nonsynonymous nucleotide substitutions per nonsynonymous site (dN) were estimated for the resulting gene alignments using the program SNAP [25]. Gene-by-gene number of informative sites and genetic diversity were obtained from the output of the PhiPack program [26]. The analyses for the codon usage variation was performed by computing the effective number of codons (Nc), which is a general measure of bias from equal codon usage in a gene. The Nc value ranges from 20 for the strongest bias (where only one codon is used for each amino acid) to 61 for no bias [27,28]. The calculation of Nc were implemented in the program CodonW 1.4 (http://codonw.sourceforge.net/ webcite).
Detection of recombination events
Since recombining fragments among aligned codon sequences have a profound effect on the detection of the positively selective evidence [29], we first tested for recombination signals between sequences in the alignment of orthologous genes. Four statistical procedures GENECONV [30], pairwise homoplasy index (PHI) [26], maximum χ2 [31] and neighbor similarity score (NSS) [32] were applied to discover the homologous recombination signals. Besides GENECONV version 1.81, the other three methods were implemented in the PhiPack package [26]. For the analyses of GENECONV, the parameter g-scale was set to 1, which allows mismatches within a recombining fragment. The p-values for inner fragments using 10,000 random permutations were used to indicate the significance of putative recombinant regions. For maximum χ2, a fixed window-size of 2/3 the number of polymorphic sites was used. For PHI, the window size was set to 100 nucleotides. Simulated p-values were estimated based on 1,000 permutations for PHI, maximum χ2 and NSS.
Detection of Selection
Maximum likelihood (ML) phylogenetic trees were reconstructed for each gene in the dataset of the core genome genes using the PhyML program [33]. A general time-reversible (GTR) model of nucleotide substitution with the ML estimates for gamma distributed rate heterogeneity of four categories (Г4) and a proportion of invariable sites were used in all tree reconstruction methods. The resulting topologies of ML trees were applied to the subsequent selection analyses.
To detect selective pressure acting on each coding gene, the rates of synonymous and nonsynonymous substitutions were estimated site-by-site using the codeml program from the PAML 4.2b package [34]. According to the topology of the resulting ML tree per gene alignment, two site-specific models that allow variable nonsynonymous (dN) and synonymous (dS) rate ratios (ω = dN/dS) among codons were applied to analyze our data set: M1a (NearlyNeutral) and M2a (PositiveSelection). Null hypothesis model M1a was nested with alternative selection model M2a. The latter model adds an extra site class for a fraction of positively selected amino acid sites with ω > 1; whereas models M1a only allows site classes with ω varying between 0 and 1 [10,35]. A likelihood ratio test (LRT) was carried out to infer the occurrence of sites subject to positive selective pressure through comparing M1a against M2a. Three replicates were run with codeml and the maximum likelihood values for each model were used in the LRT. The LRT statistic (twice the log-likelihood difference between the null and the alternative models) was compared with the χ2 distribution with two degrees of freedom. The Bayes empirical Bayes approach was employed to identify positively selected sites under the likelihood framework [36].
Mapping of positively selected sites to structure models of proteins
The web server PSORTb v3.0 was used to predict bacterial protein subcellular localization [37]. Integral beta-barrel outer membrane proteins were predicted by BOMP [38]. The three dimensional structure model of the protein encoded by the gene that showed evidence for positive Darwinian selection was modeled using the Phyre server [39]. The sites subject to positive selective pressure were mapped onto the structure and visualized by PyMol (http://www.pymol.org/ webcite).
Statistical analyses
Multiple testing correction was performed to control for Type I errors according to the approach presented by Benjamini & Hochberg [40]. For all genes tested for recombination and positive selection, q-values were calculated from p-values using the R package qvalue with the proportion of true null hypothesis set to 1 (π0 = 1) [41]. A false discovery rate (FDR) of 10% was used for the recombination analyses. As the tests used for detecting positive selection was conservative [42], an FDR of 20% was set.
The non-parametric Mann-Whitney U-test was employed to determine the significance level for the differences among the selected continuous variables (i.e., dN, dS, codon bias and nucleotide diversity) between a given COG functional categories and all other categories. Binomial test was used to estimate association between each COG category and evolutionary forces (i.e. positive selection and/or homologous recombination); Bonferroni corrections for multiple comparisons were performed according to the number of one-sided tests. The significance level was set to 5% in this study. All statistic analyses were carried out using Perl scripts and R 2.11.1 [43].
Properties of orthologous genes in 12 A. pleuropneumoniae genomes
In our recent work [19], 2,531 orthologous genes and 772 strain-specific genes have been identified in the pan-genome of 12 A. pleuropneumoniae strains using BlastClust. The above data set was used to further decode phylogenomic characterizations of pathogenic A. pleuropneumoniae. The evidence for homologous recombination and natural selection pressure whether operate on the conserved coding genes was estimated at the present study. After manually editing the aligned gene sequences and removing the low quality ones, a data set of sequence alignments of 1,960 orthologs was selected out, 81% (n = 1,587) of which were core genes that are present one copy per genome and the remaining (n = 373) were distributed genes present in at least four genomes.
The codon bias for each orthologous gene was measured by the effective number of codons (Nc value) calculated by CodonW [28]. The reduction in Nc indicates strong bias that significantly correlates with high gene expressivity [44]. A. pleuropneumoniae genes in the COG functional categories "Energy production and conversion", "Translation", "Amino acid transport and metabolism", "Nucleotide transport and metabolism", and "Carbohydrate transport and metabolism" were evident to have higher codon usage bias (P < 0.001, P < 0.001, P = 0.003, P = 0.001, and P < 0.001, respectively; one-tailed U-test) compared with genes in other COG categories. As is well known, genes bearing stronger codon bias are likely to be highly expressed and have housekeeping features [3,45]. So, high codon bias of genes present in the five COG categories is likely to elucidate the necessity of relevant coding products for implementing fundamental life cycle and essential physiological activities of A. pleuropneumoniae.
A. pleuropneumoniae genes in the functional categories "Replication, recombination and repair" and "Amino acid transport and metabolism" represented a tendency to have higher rates of synonymous (dS) nucleotide substitutions (P = 0.006 and P < 0.001, respectively; one-tailed U-test) in comparison with genes in other role categories (Table 2). On the other hand, genes in the functional categories "Replication, recombination and repair", "Amino acid transport and metabolism", "Coenzyme transport and metabolism" and "General functional prediction" showed a tendency to have higher rates of nonsynonymous (dN) substitutions (P = 0.012, P = 0.001, P = 0.007, and P = 0.007, respectively; one-tailed U-test) in comparison with genes in other COG categories (Table 2). Positive correlation was observed between dS and dN values for each COG category of A. pleuropneumoniae genes, indicating that natural selection might uniformly act on synonymous and nonsynonymous sites per gene. In addition, it was worth noting that the average dS and dN values were significantly lower (P = 0.001 and P < 0.001, respectively; one-tailed U-test) for genes in the COGs "Translation" than for genes in other COG categories. It has been suggested that genes involved in the translation machinery, e.g. ribosomal proteins and tRNA synthetases, usually evolved slowly with low dS and dN, probably due to structural and functional constraints required by the fundamental cell life cycle [20,46,47].
Table 2. The rates of synonymous (dS) and nonsynonymous (dN) nucleotide substitutions among different functional categories for A. pleuropneumoniae genes
A substantial number of genes showing evidence for recombination in the core genome of A. pleuropneumoniae
Among the 1,587 orthologous core genes, 2% (29 genes) had no occurrence of nucleotide substitutions and thus were not further investigated for evidence of homologous recombination. Furthermore, among the remaining genes, 197 gene alignments that contain few informative sites less than two could not be analyzed with programs in PhiPack and were removed from the ortholog sets. Finally, 86% of total core genes were selected to conduct the subsequent recombination analyses through four approaches. The evolutionarily conserved core genes (n = 226) were summarized (Additional file 1) and the biological functions carried out by their coding products may be essential for the survival of A. pleuropneumoniae. Notably, conserved genes were significantly enriched in the COG category "Translation" with a low Bonferroni corrected p-value (P < 0.001; Binomial test); this result was consistent with low dS and dN values mentioned before. These translation-associated protein-coding genes are generally involved in the fundamental cellular activity and thus hardly have any changes at the amino acid level as a result of functional constraints. Overall, among 12 A. pleuropneumoniae genomes, 822 orthologous core genes (52% of all 1,587 core genome genes) were identified to have significant evidence for recombination (FDR < 10%) that was detected by at least one of the four tests (Additional file 2). A total of 493, 675, 659 and 559 orthologs were identified to have recombination signals using GENECONV, Maximum χ2, NSS and PHI, respectively. Additionally, a total of 149, 148, 160, and 365 orthologs exhibiting recombination signals were identified by using one, two, three, and all four recombination tests, respectively.
Additional file 1. Highly conserved genes in the core genome of A. pleuropneumoniae. Detailed information for individual gene alignment is provided, including nucleotide diversity, informative sites, and codon bias.
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This file can be viewed with: Microsoft Excel Viewer
Additional file 2. Detailed information on test of recombination. A. pleuropneumoniae genes showing evidence for recombination detected by at least one method (FDR < 10%).
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This file can be viewed with: Microsoft Excel Viewer
It is worth noting that 23% of all core genes, which were selected as recombinants by all four methods for testing recombination, have more informative sites (P < 0.001; one-sided U-test) and higher nucleotide diversity (P < 0.001; one-sided U-test). For all core genome genes, association between COG categories and the number of genes with recombining fragments was estimated (Figure 1). Core genes that exhibit evidence for recombination were significantly overrepresented in three COG categories "Replication, recombination and repair", "Amino acid transport and metabolism", and "Inorganic ion transport and metabolism" (uncorrected P = 0.007, P < 0.001, and P = 0.029, respectively; one-sided Binomial test). However, after Bonferroni correction, only the association for the COG "Amino acid transport and metabolism" was significant (Bonferroni corrected P = 0.004).
Figure 1. Genes with evidence of recombination are enriched in three COG functional categories. The abscissa represents different COG functional categories. The ordinate represents the proportion of genes in each COG category. Bars in dark gray stand for proportions of genes (n = 365) with evidence for recombination (FDR < 10%). Bars in white stand for proportions of all core genes (n = 1,587) of A. pleuropneumoniae used in this study. Asterisks mark certain COG categories that significantly enriched with recombining genes (P < 0.05, Binomial test). The COG categories are coded as follows: J, translation; K, transcription; L, DNA replication, recombination and repair; D, cell division and chromosome partitioning; V, defense mechanisms; T, signal transduction; M, cell wall/membrane biogenesis; U, intracellular trafficking, secretion and vesicular transport; O, posttranslational modification, protein turnover and chaperones; C, energy production and conversion; G, carbohydrate transport and metabolism; E, amino acid transport and metabolism; F, nucleotide transport and metabolism; H, coenzyme metabolism; I, lipid metabolism; P, inorganic ion transport and metabolism; Q, secondary metabolites biosynthesis, transport and catabolism; R, general functional prediction only; S, function-unassigned conserved proteins; -, unknown proteins not in the COG collection.
Evidence for 57 A. pleuropneumoniae core genes subject to positive selection
The analyses of positive selection implemented in PAML was carried out for 1,587 core genome genes of A. pleuropneumoniae (in our initial experiment we included all 1,960 orthologous genes). Based on the LRT statistic for comparing the null model M1a and the selection model M2a with the distribution and corrections for multiple testing (FDR < 20%), a total of 57 genes were identified to be under strong positive selected pressure (Table 3; Additional file 3). Genes in the COG category "General function prediction only" were significantly enriched (P = 0.004; one-sided Binomial test). Except for four positively selected genes in the COG category "cell wall/membrane biogenesis", many genes with homologues in other COG categories or without homologues in the COG collection were also predicted to encode proteins localized on surface/membrane and simultaneously subject to positive selective pressure, e.g. gntT, cysW, apaA, pcaK, aphA, pqiB and ytfN.
Table 3. Genes that show evidence for positive Darwinian selection
Additional file 3. Alignments for positively selective genes. Compressed file containing all alignments for genes under positive selection (FASTA format).
Format: RAR Size: 55KB Download file
Notably, there was no obvious discrepancy for the values of dS between genes under positive selection and the remaining genes; whereas the dN values together with the number of informative sites and genetic diversity were significantly higher in the positively selected genes (P < 0.001, P < 0.001, P = 0.023; one-sided U-test). No association between positive selection and COG categories was observed, as the number of positively selected genes is low in each role category.
Among 57 positively selected genes, 24 genes also showed significant evidence for homologous recombination detected by all four recombination tests. Furthermore, 41 genes under positive selection pressure showed evidence for recombination identified by at least one test. It indicates that positive selection should be associated with intragenic recombination, as recombination can lead to phylogenic incongruence and highly false positives when selective pressure on protein coding sequences was estimated [3,29].
Gene acquisitions and losses that contribute to the virulence and serotypic diversification of A. pleuropneumoniae have been depicted in detail [18,19], but our understanding on small genetic variations caused by positive selection and homologous recombination, which also factually influence the evolutionary trajectories of protein coding genes, has not been well considered for this swine pathogen so far. In this report, we chose 12 genomes of A. pleuropneumoniae to study the evolutionary driving forces acting on the core genome of this animal pathogen using a comparative phylogenomic approach.
Intragenic recombination and positive selection both play a key role in the evolution of A. pleuropneumoniae pan-genome
Tests for intragenic homologous recombination and positive selection were performed with 1,587 orthologous genes present in the core genomes of twelve strains of A. pleuropneumoniae. Overall, our results indicated that about a quarter of the genes in A. pleuropneumoniae core genome showed significant evidence for intragenic recombination. In comparison, core-genome recombination was also evident in both species of the genus Streptococcus, as 18% and 37% of the core genome for S. agalactiae and S. pyogenes, respectively, showed evidence for homologous recombination [5]. Notably, in A. pleuropneumoniae, two COG categories "Replication, recombination and repair" and "Amino acid transport and metabolism", which both presented high values of dS and dN, were favored by intragenic recombination.
On the other hand, 57 A. pleuropneumoniae genes, accounting for approximately 3.6% of the core genome, were identified to be undergoing positive selection. Another similar study on the identification of genes under positive selection in E. coli reported that 0.7% of 3,505 genes found in six E. coli genomes showed evidence for positive selection and no evidence for recombination [1]. Like other pathogenic bacteria, a substantial number of positively selected genes in A. pleuropneumoniae encode protein products involved in the biogenesis and structural components of bacterial cell wall and/or outer membrane. These genes are likely to be associated with co-evolutionary arms races between pathogenic microorganisms and hosts. To further decipher the roles of evolutionary pressure operating on the core genome of A. pleuropneumoniae, we analyzed the functional properties of the positively selected genes and potentially important residues subject to positive selection.
Genes subject to positive selection in A. pleuropneumoniae
We found that many protein products encoded by the positively selected genes were exposed on the cell surface or involved in structural constituents of bacterial cell wall. Some of these proteins have been reported to be important virulence factors associated with bacterial adherence, colonization and persistence. Therefore, it suggests that the genes under diversifying selection may dynamically interact with the host immune and defense systems.
The beta barrel porins are pore proteins that allow the passive diffusion of small, hydrophilic, or changed molecules across Gram-negative bacterial outer membranes [48]. The pore proteins have been believed to be crucial for not only dynamic interactions with the host immune system, but bacterial pathogenesis as well [1,49]. An outer membrane protein OmpP2, which was predicted to be beta barrel porin, showed strong evidence for positive selection with a low q-value (Table 3). The results of the Bayes empirical Bayes (BEB) analyses showed that A. pleuropneumoniae OmpP2 amino acid residues 306, 317, and 320 were subject to intense positive selective pressure (Figure 2). The three residues all located on a predicted extracellular loop in the C-terminus, perhaps associated with potential antigenic epitope. In addition, OmpP2 has been experimentally confirmed to be essential for in vivo survival of A. pleuropneumoniae by signature-tagged mutagenesis and also an immunogenic surface antigen by the immunoproteomic approach [50,51]. In our initial selection analyses using a set of 1,960 genes, gene fepA present in 11 A. pleuropneumoniae genomes encodes a beta barrel porin (Figure 2) and was also identified with evidence for positive selection (data not shown). FepA of A. pleuropneumoniae shared a common TonB-dependent receptor plug domain (PF07715) with E. coli outer membrane protein FepA that is a receptor for ferric enterobactin and for colicins B and D [52]. FepA of A. pleuropneumoniae has already been reported to exhibit immunogenic activity [53]. The adaptive changes in both porins might be beneficial for A. pleuropneumoniae to escape from the host immune systems and attack of phages, antibiotics, and colicins.
Figure 2. Three-dimensional structural models of beta barrel porins OmpC and FepA. Orange spheres stand for amino acid sites that are subject to strong positive selection (posterior probability > 95%).
Bacterial surface polysaccharides, which are often involved in adherence and colonization, may be directly exposed to the host immune pressure. Three A. pleuropneumoniae genes (hcsA, hcsB, and wecF) participated in biogenesis of surface polysaccharides showed significant evidence for positive selection. The products of selected genes hcsA and hcsB code for capsule polysaccharide modification proteins that share 63% and 64% identity with Haemophilus influenzae HcsA and HcsB, respectively, which facilitate transport of capsular polysaccharide across outer membrane and are essential for bacterial virulence [54]. Besides, the positively selected gene wecF codes for a 4-alpha-L-fucosyltransferase and is located at a wec locus which has highly conserved colinearity in all A. pleuropneumoniae genomes. The products of wecF together with other wec genes exhibit high similarity to the E. coli K12 homologues that are involved in the assembly of a cell surface glycolipid [55].
The other gene apaA encoding an antigenic membrane lipoprotein that could provide cross-protection against heterologous A. pleuropneumoniae serovars [56], was also under strong positive selection (q-value = 0.062). The above analyses strongly demonstrated that the positively selected genes involved in the biosynthesis and structural composition of cell surface/wall have undergone adaptive functional changes, perhaps allowing bacterial pathogens to escape recognition by the host immune system and phages. Such phenomena have already been proposed by the previous studies of natural selection on the E. coli genome [1,14].
The proteases of A. pleuropneumoniae have been reviewed to be one of important virulence factors and contribute to pathogenesis [57]. Overall, 4 protease genes (i.e., ptrA, lonH, sppA and tldD) showed significant evidence for positive selection. The precise function of these protease genes identified here, to our knowledge, was not well understood for this pathogen. However, proteolytic enzymes are pivotal to the secretion processes of Gram-negative pathogens and several of them have been described as attractive drug targets in other pathogens, e.g. ClpP [58] and Lon [59].
Our findings indicated that intragenic homologous recombination and positive Darwinian selection, unsurprisingly, indeed play crucial roles in the evolution of pathogenic A. pleuropneumoniae. In genes with extensive functional classification we found genes involved in the formation of cell surface/membrane are favored by the positive selective pressure. The adaptive changes in these positively selected genes and/or residues likely attribute to dynamic interaction caused by the host immune and defense systems. Of course, the diversifying selective forces of genes encoding metabolic functions may be also advantage for improving bacterial fitness in response to a variety of environmental signals. More experimental works are required for verifying the functions of these adaptive genes in future. Overall, the genetic evidence of positive selection will provide promising targets for further researches in the mechanisms of immune evasion and the host-pathogen interaction in A. pleuropneumoniae.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
ZX carried out the data collection, data analyses, wrote the manuscript. ZX and RZ participated in its design and revised the manuscript. RZ and HC supervised and coordinated the project. All authors read, edited and approved the final manuscript.
This work was supported by the National Natural Science Foundation of China (30901075).
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text:
Suse 8.2 cannot compile Kernel Module because of wrong version of gcc???
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Suse 8.2 cannot compile Kernel Module because of wrong version of gcc???
I have been running version 4496 for some time now without trouble. Yesterday I used the update utility and my kernel got updated. The update told me that I would probably need to re-install the NVIDIA driver. No Problem I thought.....
Here is the output from the failed install log...
nvidia-installer log file '/var/log/nvidia-installer.log'
creation time: Thu Aug 14 14:16:44 2003
option status:
license pre-accepted : false
update : false
force update : false
expert : false
uninstall : false
driver info : false
no precompiled interface: false
no ncurses color : false
query latest driver ver : false
OpenGL header files : false
no questions : false
silent : false
XFree86 install prefix : /usr/X11R6
OpenGL install prefix : /usr
Installer install prefix: /usr
kernel include path : (not specified)
kernel install path : (not specified)
proc mount point : /proc
ui : (not specified)
tmpdir : /tmp
ftp site :
Using: nvidia-installer ncurses user interface
-> License accepted.
-> No precompiled kernel interface was found to match your kernel; would you li
ke the installer to attempt to download a kernel interface for your kernel f
rom the NVIDIA ftp site (
)? (Answer: No)
-> No precompiled kernel interface was found to match your kernel; this means
that the installer will need to compile a new kernel interface.
-> Kernel include path: '/lib/modules/2.4.20-64GB-SMP/build/include'
-> Cleaning kernel module build directory.
executing: 'cd ./usr/src/nv; make clean'...
rm -f nv.o os-agp.o os-interface.o os-registry.o nv-linux.o nv_compiler.h *
.d NVdriver nvidia.o
-> Building kernel module:
executing: 'cd ./usr/src/nv; make nvidia.o SYSINCLUDE=/lib/modules/2.4.20-64
You appear to be compiling the NVIDIA kernel module with
a compiler different from the one that was used to compile
the running kernel. This may be perfectly fine, but there
are cases where this can lead to unexpected behaviour and
system crashes.
If you know what you are doing and want to override this
check, you can do so by setting IGNORE_CC_MISMATCH.
In any other case, set the CC environment variable to the
name of the compiler that was used to compile the kernel.
[1;31m*** Failed cc sanity check. Bailing out! ***
[0mmake: *** [gcc-check] Error 1
-> Error.
ERROR: Unable to build the NVIDIA kernel module.
ERROR: Installation has failed. Please see the file
'/var/log/nvidia-installer.log' for details. You may find suggestions
on fixing installation problems in the README available on the Linux
driver download page at
I ran the following to find out which version of gcc was used to compile the kernel...
cat /proc/version
Linux version 2.4.20-64GB-SMP (root@SMP_X86.suse.de) (gcc version 3.3 20030226 (prerelease) (SuSE Linux)) #1 SMP Wed Aug 6 18:30:02 UTC 2003
and the following for gcc...
gcc -v
Reading specs from /usr/lib/gcc-lib/i486-suse-linux/3.3/specs
Configured with: ../configure --enable-threads=posix --prefix=/usr --with-local-prefix=/usr/local --infodir=/usr/share/info --mandir=/usr/share/man --libdir=/usr/lib --enable-languages=c,c++,f77,objc,java,ada --disable-checking --enable-libgcj --with-gxx-include-dir=/usr/include/g++ --with-slibdir=/lib --with-system-zlib --enable-shared --enable-__cxa_atexit i486-suse-linux
Thread model: posix
gcc version 3.3 20030226 (prerelease) (SuSE Linux)
seems the same version to me... any thoughts???/
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A lot of people were wondering why the Times endorsed Christine Quinn and went out of its way to publish unflattering stories about her competitors... Well it turns out that they were making a short documentary about Quinn's campaign the whole time! Shouldn't that have been disclosed a hell of a lot earlier?
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Sussegado in Portuguese means laid back or chilled out and that is exactly how you feel as you make your way past the lawns at the Park Hotel to the beach. The Shack does not need much of a makeover as the sounds of the waves crashing and the feeling of cool sand at your feet provide the perfect ambience to try out Goan delicacies. To pamper the senses further, two guitarists from Goa play classical folk tunes.
Goa is today a potpourri of cultures and the food available also reflects this mix. However, no trip to Goa is complete without tasting its traditional food which is a mix of local Konkani and Marathi flavours with a strong Portuguese influence.
It is these traditional flavours that Chef Carmo Peter and Chef J. Saldanha form Goa will lay out for gourmands in the city to relish.
As you settle down with a refreshing drink in hand and the delicious seafood starters arrive you will have to keep reminding yourself that you are in Vizag and not on holiday in Goa. If you have been to the beautiful State before, then dishes like Lamb Vindalho, Chicken Xacuti, Chicken Cafreal and Bebinca are sure to make you relive your holiday.
For those sampling it for the first time it is a culinary cruise that combines local-Goan ingredients with its rich Portuguese heritage.
Chef Saldanha, who has conducted many food festivals across the country and abroad, says although there is a Mediterranean influence in the cuisine they do not use ingredients like olive oil which is used in Portuguese food.
For lovers of seafood the festival is a must visit. Fish curry and prawn curry Goan style, kingfish, and spicy and sour shark will be in offer. The buffet will be cyclical and will be repeated every four days.
For vegetarians dishes like Alami Sukem (mushroom chilli fry), Xengo tempered (drumsticks curry) Beringelas Picante (spicy aubergines), and Goan dal fry will be among the dishes on offer.
For desert delicacies like bathika, dodol and bebinca will be laid out. The staff at the restaurant and the chefs know the cuisine very well and offer detailed explanations about every dish.
You not only get to taste the cuisine you get educated about it as well. As you leave the restaurant you are sure to be humming the popular folk song 'Ya ya maya ya' all the way home.
For dinner reservations and details contact Abhijeet D'Souza at 9966721388. The buffet starts at 8 p.m and is on till March 24.
Keywords: Goan cuisine, food festival


text:
"I didn't want to tell anyone," he said. "Like most guys, I was skeptical that it was true. I came from a family of three and she of four, so I so no reason why we would a problem conceiving."
Ceizyk said it was true men "don't understand their own biology," and many of the doctors "looked at it like it was a non-issue and something to just overcome."
"My sperm count was a million -- I thought that was plenty," he said. "Why did I need a $1,000 process? It was ignorance. Forty million [sperm count] is the bare minimum and I was way under."
Ceizyk wrote a memoir about the couple's $70,000 ordeal, "Almost a Father," and writes a blog with the same name.
In six years, they had 11 embryo transfers, five IVFs, six infertility doctors, three miscarriages and two cross-country trips to a specialty hospital in New Jersey. They finally conceived their now-11-year-old daughter in an intracytoplasmic sperm injection procedure, extracting sperm that was injected into one of his wife's eggs.
Attending support groups early on, there were no other men and "in some cases they weren't allowed," he said.
The couple started their own Resolve support group in Tucson.
"It saved me," he said. "It was normalizing and to have the commonality of experience with other men.
"It ended up being a team effort and, by starting to actually learn the [medical] terminology and participate in conversations, we got much closer," he added. "It ended up being a team effort.
"My wife is my true hero," he said. "She was the one who gave me the courage to evolve into a team player in our parenthood pursuit, and we have run a couples support group helping other couples find a way to develop a new form of intimacy in their baby-making pursuits, even when they include a staff of doctors and medical protocols."


text:
Complete the 3 forms below prior to requesting letters of recommendation from Teachers and Counselors. Remember you need to give the Teacher(s)/Counselor who will be writing your letter of recommendation a minimum 10 school days advance notice for your application to be completed on time.
College applications can be a confusing task, so here are a couple tools to help you clarify the process:
College > College Applications >


text:
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Various thoughts and adventures, including but not limited to Linux, assorted bits of hardware new and old, and occasionally Windows XP/Vista/7.
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