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MANF and CDNF are evolutionarily conserved neurotrophic factors that specifically support dopaminergic neurons. To date, the receptors and signalling pathways of this novel MANF/CDNF family have remained unknown. Independent studies have showed upregulation of MANF by unfolded protein response (UPR). To enlighten the role of MANF in multicellular organism development we carried out a microarray-based analysis of the transcriptional changes induced by the loss and overexpression of Drosophila Manf.
The most dramatic change of expression was observed with genes coding membrane transport proteins and genes related to metabolism. When evaluating in parallel the ultrastructural data and transcriptome changes of maternal/zygotic and only zygotic Manf mutants, the endoplasmic reticulum (ER) stress and membrane traffic alterations were evident. In Drosophila Manf mutants the expression of several genes involved in Parkinson's disease (PD) was altered as well.
We conclude that besides a neurotrophic factor, Manf is an important cellular survival factor needed to overcome the UPR especially in tissues with high secretory function. In the absence of Manf, the expression of genes involved in membrane transport, particularly exocytosis and endosomal recycling pathway was altered. In neurodegenerative diseases, such as PD, correct protein folding and proteasome function as well as neurotransmitter synthesis and uptake are crucial for the survival of neurons. The degeneration of dopaminergic neurons is the hallmark for PD and our work provides a clue on the mechanisms by which the novel neurotrophic factor MANF protects these neurons.
Recently characterised MANF (Mesencephalic Astrocyte-derived Neurotrophic Factor) and CDNF (Cerebral Dopamine Neurotrophic Factor) form an independent family of neurotrophic factors [1]. MANF was identified from a conditioned media of cultured mesencephalic astrocytes in a search for secreted factors supporting dopamine (DA) neurons [2]. Specific loss of DA neurons is the characteristic feature of Parkinson's disease (PD). Therefore factors that maintain and support DA neurons are attractive candidates for the treatment of PD. MANF was shown to support the survival of cultured primary DA neurons but to have no effect on cultured GABAergic or serotonergic neurons [2]. Subsequently, mammalian MANF and its paralog CDNF were shown to prevent the loss of DA neurons in mouse 6-OHDA PD model [3,4]. Contrary to other vertebrate neurotrophic factors the MANF/CDNF family is evolutionarily well conserved among multicellular organisms including the fruit fly, Drosophila melanogaster [2,3,5]. Importantly, the protective role of MANF for DA neurons is also conserved [5]. Apparently both mammals and invertebrates share the same signalling partners as the lack of Drosophila Manf can be substituted by human MANF [5]. However, the interaction partners or how these proteins act at the molecular level are still elusive. It is important to understand the mechanisms of how these MANF/CDNF family proteins work at molecular level before the potential therapeutic applications.
Recent studies have shown the protective role of mammalian MANF (also known as ARMET) to be more general than restricted to the nervous system. MANF is upregulated by UPR in several mammalian cell lines [6-8] and by ischemia induced UPR in the heart and brain [8-10]. ER is the central regulator of protein folding and quality control and it has to adapt its capacity to the specific need of a particular cell type. Conditions challenging the function of the ER, like an increase of newly synthesized unfolded proteins in its lumen, lead to UPR [11]. In eukaryotes, the three canonical branches of UPR are mediated by ER membrane-associated sensor proteins. In stress-free, functional ER the intralumenal domains of these sensor proteins are bound to a chaperone BiP/GRP78 (Binding immunoglobulin protein/Glucose-regulated protein 78) and maintained inactive [12,13]. The UPR intersect with a variety of inflammatory and stress signalling pathways and networks activated by oxidative stress, all of which can influence cell metabolism. ER stress and UPR have also been implicated in the pathogenesis of several neurodegenerative diseases because of their characteristic accumulation of specific misfolded proteins [14]. Data from PD patients reveal that in DA neurons of substantia nigra the UPR is activated [15]. Recently, Drosophila has been used progressively to model human neurodegenerative diseases and UPR [16-18].
We have previously generated and characterized Drosophila Manf mutants. The zygotic null mutants (ManfΔ96) survive up to 2nd instar larvae due to the high maternal contribution. Mutants lacking both maternal and zygotic Manf (ManfmzΔ96) are late embryonic lethal and never hatch [5]. The embryonic lack of maternal Manf gene products and the lethality is rescued by paternal Manf gene expression. Both ManfmzΔ96 and ManfΔ96 mutants share nervous system defects, particularly dopamine neurites are altered and degenerate. Ectopic overexpression of Manf reveals no evident abnormalities in the embryonic or larval nervous system development or in the adult flies (data not shown).
Here we compare the mRNA expression profiles of ManfmzΔ96 mutant embryos, ManfΔ96 mutant larvae, paternally rescued maternal mutant embryos ManfmΔ96/+, and Manf ubiquitously overexpressing larvae to the wild type animals of exactly the same stages.
Results and discussion
The most prevalent changes in gene expression occur in Manf mutants that lack the maternal contribution of Manf
For microarray gene expression analysis we used two developmental stages in combination with three separate genotypes. The age of embryos and larvae were selected according to the lifespan of the Manf mutants. ManfmzΔ96 mutants fail in tracheal air filling and never hatch. Mutant ManfmzΔ96 embryos were picked during the late stage of 17 (21-22 hours after egg laying, AEL) just before hatching when the trachea of wild type embryos fill with air. Mutant ManfΔ96 larvae with maternal contribution survive to approximately 75 hours AEL and were collected as first instars 29-50 hours AEL when maternal loads of Manf gene products have decreased. Three biologically independent sets of samples were used for microarray analysis. The expression profiles of all transgenic and mutant animals were compared to the wild type of the corresponding developmental stage. The numerical overview of statistically significant differences (P < 0.01) showed the most prominent changes in gene expression of ManfmzΔ96 mutants (about 10% of genes differentially regulated). The smallest changes took place in the case of paternal rescue (less than 0.5% of genes differentially regulated) (Table 1). Among the differentially regulated genes, approximately half were up- or downregulated in different Manf conditions. Altogether we validated 40 genes of the microarray results. Genes were selected by several criteria such as differential expression or similar regulation in both mutants or otherwise high representation in the whole dataset. As a result, 61.5% of validated genes in different genetic conditions were independently confirmed by qPCR (Tables 2 and 3, Additional file 1: Table S1).
Table 1. Overview of microarray experiment
Table 2. qPCR validation of results and microarray data obtained from stage 17 embryos
Table 3. qPCR validation of results and microarray data obtained from 29-50 hr larvae
Additional file 1. Table of all obtained qPCR results. The Excel file contains two separate sheets, one for the data obtained from stage 17 embryos and the other for the data obtained from 29-50 hr AEL larvae.
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Membrane transporters and metabolic genes are downregulated in ManfmzΔ96 mutants
Development of maternal and zygotic mutant ManfmzΔ96 proceeds until stage 16 with no differences to wild type embryos, but 21 hours AEL the cuticle and the nervous system defects become evident [5]. In comparison to wild type embryos of the same age, in ManfmzΔ96 mutants 1191 genes were found to be downregulated. These genes were grouped into 105 functional clusters (Additional file 2), among which the most significantly enriched clusters were related to membrane transporters (25 genes) and transmembrane proteins (146 genes) (Table 4). There were several enriched clusters referring to different metabolic processes such as amine, amino acid and carboxylic acid catabolic processes (11 genes), DNA metabolic processes (26 genes), and genes related to pyrimidine metabolism (15 genes).
Additional file 2. DAVID functional annotation clustering analysis of downregulated genes in embryonic ManfmzΔ96 mutants. An Excel file; 1191 genes were downregulated and grouped into 105 functional clusters.
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Table 4. GO clustering analysis of downregulated genes in ManfmzΔ96 mutants
Among the downregulated genes in ManfmzΔ96 mutants, the gene ontology (GO) term of mitochondria-related transcripts was highly enriched (28 genes). Mitochondria are the respiratory machinery of the cell responsible for oxidation processes and participate in maintaining cellular homeostasis. The lack of Manf causes downregulation of several components in all mitochondrial compartments: the lumen as well as the inner and outer membranes.
Stress and defence response related genes are induced in ManfmzΔ96 mutants
In ManfmzΔ96 mutant embryos, 1243 genes were upregulated in comparison to wild type embryos of the same stage (Additional file 3). The most prominent group of significantly enriched GO terms was immune and defense response (69 genes), followed by the groups related to proteolysis, hydrolases and peptidases (197 genes) (Table 5). The upregulated gene set was also enriched in genes related to actin cytoskeleton organization and actin filament-based process (28 genes). Moreover, genes involved in cell death (28 genes) were prominently enriched as well.
Additional file 3. DAVID functional annotation clustering analysis of upregulated genes in embryonic ManfmzΔ96 mutants. An Excel file; 1243 genes were upregulated and grouped into 230 functional clusters.
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Table 5. GO clustering analysis of upregulated genes in ManfmzΔ96 mutants
Enzymes for dopamine synthesis are upregulated despite of low dopamine levels
Extremely low dopamine levels are detected in ManfmzΔ96 embryos [5]. Nonetheless, we observed significant upregulation of transcripts of the dopamine producing enzymes tyrosine hydroxylase (TH) and DOPA decarboxylase (Ddc) (Table 2). Punch; encoding a GTP cyclohydrolase, an enzyme for tetrahydrobiopterin (a cofactor for TH) synthesis was also upregulated in ManfmzΔ96 embryos (Table 2). There could be several explanations for these alterations. Tyrosine, the essential amino acid for dopamine synthesis, is transported into the cell from hemolymph. In Manf mutants several amino acid membrane transporters were downregulated. The lack of substrate, tyrosine, together with low amounts of the end product, dopamine, could lead to the upregulation of the genes coding for the enzymes in dopamine synthesis pathway and their cofactors.
Genes involved in nucleic acid metabolism and protein folding are downregulated in larval ManfΔ96 mutants
Larval ManfΔ96 mutants with maternally contributed Manf gene products never reach 3rd instar stage and rarely survive up to 75 hours AEL at 25°C. Initially, ManfΔ96 mutant larvae hatch and feed normally. Whereas wild type larvae grow rapidly, the mutant larvae remain smaller and start to wander away from food, become sluggish and stop moving, still responding to touch and usually die during the first larval molt [5]. Because of the temporal differences in survival between individual ManfΔ96 mutant larvae from 1st to 2nd instar, for microarray analysis we collected larvae 29-50 hours AEL.
When comparing the expression profile of larval ManfΔ96 mutants to the wild type larvae, almost half the number of genes (690) was significantly downregulated as compared to the rate in ManfmzΔ96 embryos resulting in 140 functional clusters (Additional file 4). The most enriched GO terms fell into clusters related to intracellular organelle lumen and nucleic acid metabolic processes (Table 6). The cellular activities such as DNA replication, RNA processing and splicing were enriched among downregulated genes. The 5th highly enriched cluster consisted of GO terms such as ER related genes (24 genes), proline and arginine metabolism (9 genes), and oxioreductases (9 genes). Mitotic cell cycle, chromosomal segregation, and mitotic spindle organization were also clustered as significantly enriched. These changes could be linked to UPR, as one of the outcomes of UPR is general and unspecific downregulation of novel protein synthesis, at the same time activating the protein synthesis for chaperones and genes enhancing the protein folding to release the unfolded protein load in ER.
Additional file 4. DAVID functional annotation clustering analysis of downregulated genes in larval ManfΔ96 mutants. An Excel file; 690 genes were downregulated and grouped into 140 functional clusters.
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Table 6. GO clustering analysis of downregulated genes in ManfΔ96 larval mutants
Sugar metabolism, hydrolases, and ER related oxidation reduction genes are induced in ManfΔ96 larvae
In ManfΔ96 larval mutants, 682 genes showed upregulation in comparison to the wild type larvae. The most enriched functional clusters included GO terms like sugar metabolism and glucosidases, glycosyl hydrolases (18 genes), and hydrolases and carboxylesterases (23 genes), followed by cluster of monooxygenases, Cytochrome P450, iron, vesicular fraction, oxidation reduction and endoplasmic reticulum (49 genes) (Additional file 5). Chitin and polysaccharide metabolism was also among the highly enriched GO terms (40 genes). The 5th ranked cluster of GO terms was immune and defence response (19 genes), which was the most highly enriched cluster in ManfmzΔ96 mutant embryos.
Additional file 5. DAVID functional annotation clustering analysis of upregulated genes in larval ManfΔ96 mutants. An Excel file; 682 genes were upregulated and grouped into 122 functional clusters.
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Genes related to RNA metabolism, ATP binding, and DNA replication are downregulated in both Manf mutants
Next, we looked for functional terms among the 208 commonly downregulated genes in both Manf mutants (Additional file 6). There was 30% of overlap in gene sets between the ManfΔ96 and ManfmzΔ96 mutants. Among the downregulated overlapping genes, the enrichment of GO terms fell into RNA metabolism and ribosome biogenesis (19 genes). Around 10% of all known ATP binding genes were downregulated (28 genes) together with 14 genes of the purine and pyrimidine metabolism. Additionally, the genes coding sugar transporters and the genes involved in transmembrane transport (7 genes) highly represented among downregulated genes in ManfmzΔ96 mutant embryos, were repressed in zygotic mutant ManfΔ96 as well. GO terms related to DNA replication (15 genes) were also enriched among the commonly downregulated genes in both Manf mutants.
Additional file 6. DAVID functional annotation clustering analysis of common downregulated genes in ManfmzΔ96 and ManfΔ96 mutants. An Excel file; 208 genes were downregulated in both Manf mutants and grouped into 47 functional clusters.
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Altogether, the ManfmzΔ96 mutant embryos lacking both maternal and zygotic Manf showed twice more drastic decline from wild type transcriptome than ManfΔ96 larval mutants, whose maternal transcripts gradually diminish. Beside behavioural and growth phenotype, ManfΔ96 larvae show degeneration of dopaminergic neurites in ventral nerve cord [5]. We found three genes downregulated in both mutants that are involved in neurite development: Abelson tyrosine kinase (Abl), Guanine nucleotide exchange factor GEF64C (Gef64C) and the transcription factor longitudinals lacking (lola).
A third of all upregulated genes (229) were induced in both mutants (Additional file 7). Immune and defense response was the most enriched functional cluster (29 genes) along with the group consisting of monooxygenases, oxidoreductases, vesicular fraction, endoplasmic reticulum, Cytochrome P450 and lipid metabolic process (21 genes). Controversially, disabled (Dab, substrate of Abl), was upregulated among the 10 genes involved in neuronal development e.g. transcription factor Krüppel (Kr), negative regulator of growth shrub (shrb), insulin receptor (InR), and Drosophila extracellular-signal-regulated kinase (ERK) rolled (rl).
Additional file 7. DAVID functional annotation clustering analysis of common upregulated genes in ManfmzΔ96 and ManfΔ96 mutants. An Excel file; 229 genes were upregulated in both Manf mutants and grouped into 45 functional clusters.
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Genes related to UPR were upregulated in Manf mutants
Previous in vitro studies using tunicamycin, the inhibitor of glycosylation, to induce ER stress in mammalian cell lines have shown in UPR the upregulation of MANF [6,7]. In rat neonatal cardiomyocytes in response to UPR MANF is secreted to promote cellular survival [8]. ER stress and one of the consequences, UPR, has been mainly studied in yeast and mammalian cells. In Drosophila, there are several recent studies where UPR has been addressed [16,19]. Manf has been shown to be upregulated after feeding tunicamycin to adult fruit flies indicating the involvement of Manf in chemically induced UPR in Drosophila [20].
To find out the intracellular localisation of Manf in Drosophila, we used larval 2nd instar garland cells. Garland cells are nephrocytes with high rate of endocytosis and express several neuronal and exocytosis markers e.g. prospero (pros, mammalian Prox-1 homolog), SNARE binding protein Ras opposite (Rop) facilitating neurotransmitter secretion, and Syntaxin 1A (Syx1A, a t-SNARE) [21,22]. These cells have the most abundant expression of Manf starting from embryogenesis [5]. In the garland cells, Manf was localised around the nucleus, partially overlapping with ER-targeted marker (Figure 1A-C).
Figure 1. Manf localises intracellularly partially to ER and endosomal compartment. A-C - The confocal micrographs of 2nd instar larval garland cells stained for α-Manf (magenta) showing Manf expression around the nuclei (A) overlapping partially with ER-EYFP marker (green), DAPI (blue) was used to stain nuclei (A-C). D - Western blot analysis shows two fold increased amount of phosphorylated elF2α in ManfmzΔ96 embryos in comparison to wild type w1118 embryos. Decreasing amounts of samples were loaded to obtain the optimal result for quantification; the triangles represent the direction of decrease in loading. α-tubulin (α-tub) was used as a loading control. E-G - The confocal micrographs of Schneider-2 cells transfected with Manf cDNA construct and stained with Lysotracker (green) and α-DmManf show almost no colocalisation (less than 0.3%). H-M - The confocal micrographs of the wild type 3rd instar larval fat body expressing GFP-tagged UAS-constructs (green) driven by fat body specific ppl-GAL4 and stained for α-DmManf (magenta); nuclear stain DAPI (blue) was used. In H-J Manf localises close to clathrin coated vesicles marker GFP-clathrin light chain (Clc). In K-M Manf shows partial colocalisation with late endosomal compartment marker Rab7. N-S - In the salivary gland cells of 3rd instar larvae Manf (magenta) localises close to the basal cell borders and colocalises partially with early endosomal marker Rab5 (green) (N-P) and the recycling endosomal pathway marker Rab11 (green) (Q-S). Close arrows mark the cell borders and the open arrows mark the areas of colocalisation; all images consist of single laser confocal section. Scale bars: in A-C 2 μm, 4 μm in H-J, 5 μm in E-G and K-S.
Next, we tested the hypothesis that the metabolic changes in Manf mutant could be the result of severe ER stress caused by altered expression of ER related genes. Drosophila genes homologous to several ER stress pathway have been identified. Out of 30 genes involved in ER and protein processing in the KEGG database, 24 have one or more homologues in fruit flies (Figure 2, Table 7). Of these UPR related Drosophila genes, 30% showed altered gene expression in our microarray experiment. Altogether, 29 genes involved in ER and protein processing show statistically significant expression changes. The gene CG10420 is an annotated gene with unknown function in Drosophila. Its human homologue nucleotide exchange factor SIL1 (S. cerevisiae ER chaperone homologue) is a BiP binding protein. In humans, several mutations in SIL1 gene disrupting the protein cause the Marinesco-Sjögren syndrome (MSS), an autosomal recessive cerebellar ataxia complicated by cataracts, developmental delay and myopathy [23]. We validated CG10420 by qPCR as downregulated by Manf overexpression and upregulated when Manf is abolished in Drosophila embryos and larvae. It has been shown by immunoprecipitation studies that mammalian MANF binds to BiP [24]. Thus it is possible that Manf and CG10420 compete in binding to BiP together with unfolded proteins. As the ectopic overexpression of Manf has no effect on fruit fly viability or nervous system development (data not shown), the diminished transcript level for CG10420 is not comparable to the total lack of this gene product in the MSS patients. According to our qPCR validated microarray results several other genes implicated in UPR were downregulated in larvae overexpressing Manf, such as pancreatic eIF-2α kinase (PERK), Heat shock protein 83 (Hsp83), Ubiquilin (Ubqn), and septin interacting protein 3 (sip3). In embryonic ManfmzΔ96 mutants all above mentioned genes were significantly upregulated as well as considerable number of other ER chaperone genes (Table 7). Furthermore, when evaluating the ultrastructural changes in ManfmzΔ96 mutants, we noticed that the ER was swollen and dilated in epidermal cells, indicating severe disturbances of ER structure (Figure 3A-C). In ManfmzΔ96 mutant embryos the extent of phosphorylated eukaryotic initiation factor eIF2α was more than two fold upregulated when compared to the wild type (Figure 1D) indicating the presence of UPR in these Manf mutants. The phosphorylation of eIF2α by PERK is a hallmark for UPR, resulting in reversible blockage of translation and downregulation of the protein load to the ER [25]. In Drosophila there are two kinases, PERK and Gcn2, shown to be able to phosphorylate eIF2α [26,27]. The expression of Gcn2 is high only during early stages of embryogenesis [27]. Thus PERK is a potential candidate kinase behind eIF2α phosphorylation at the end of embryogenesis. Interestingly, our microarray data showed that in ManfmzΔ96 mutants the transcription of PERK was [25] upregulated and the genes involved in different metabolic processes such as amino acid, DNA and pyrimidine metabolism were downregulated indicating overall inhibition of translation. So it is probable that the UPR PERK pathway is activated in ManfmzΔ96 mutants. The second UPR sensor, IRE1, activates two separate downstream branches. One of the branches leads to the activation of Jun kinase and death pathway [28]. According to the microarray results, Drosophila Jun kinase kinase hemipterous showed significant upregulation in both Manf mutants.
Figure 2. Protein processing in endoplasmic reticulum is altered in Manf mutants. An online coloured KEGG pathway scheme showing altered gene expression in red (upregulation), blue (downregulation), or purple (altered gene expression, differences between the two mutants) boxes. The unaltered known Drosophila homologues to identified components from other organisms are presented in green-filled boxes. The complete list of altered genes is summarised in Table 7. Notice the upregulation of genes encoding BiP/GRP78 chaperone binding proteins and components in ER leading to terminally misfolded protein degradation pathway. Out of the three branches of ER stress, IRE1 pathway leading to cell death shows upregulation in both mutants as PERK pathway is upregulated in maternal and zygotic Manf mutants.
Table 7. List of genes with altered expression according to microarray analysis related to KEGG pathway of protein processing in ER
Figure 3. Manf mutants show severe defects in vesicular traffic in the cells with high secretion or endocytosis rate. A-C - In the epidermal cells of stage 17 ManfmzΔ96 mutant embryos compared to wild type (w), ER is rounded and swollen (open arrow), there are multiple vesicles stuck close to plasma membrane (thick arrow), and the cuticle (cu) is severely disorganized. Cuticular layers are indicated by a line. nu = nucleus D-E - High pressure freezing TEM images of 1st-2nd instar larvae show no difference in the layering of the cuticle between wild type and ManfΔ96 mutant. The cellular membranes are weakly stained by this technique. Notice the unattached first instar cuticle in ManfΔ96 mutant (cu' in E). F-G The comparison of wild type and ManfΔ96 mutant garland cells shows excessive accumulation and enlargement of α-vesicles (α) and dilated ER. The labyrinth channels and slit membranes are similar between wild type and ManfmzΔ96 mutant (white open arrows). H-I Secretory cells of gastric caeca in ManfmzΔ96 mutant show accumulation of vesicles full of debris (white arrow heads) never found in wild type. Scale bars: in A-E 500 nm, 1 μm in F and G, 2 μm in H and I.
Conclusively, the absolute lack of Manf results in severe ER stress and upregulation of many genes involved in UPR finally leading to the cell death. When maternal Manf stores are gradually decreased in ManfΔ96 larvae, there are only few genes upregulated that are related to ER: CG10420, ubiquitin protein ligase lin19, heat shock protein cognate 1 and ubiquitin-conjugating enzyme CG5823. As our data come mostly from gene expression analyses, further biochemical experiments are needed to identify the exact role of Manf in UPR.
Lack of Manf results in downregulation of several genes in exocytosis pathway
Ultrastructural study of ManfmzΔ96 mutants revealed overload of vesicles next to the apical part of epidermal cells and reduced microvillae thought to enhance the capacity of the secretion of these cells (Figure 3C). This result, together with the severe defects observed in the cuticle secretion and organisation (Figure 3B, C), suggested a possible involvement of the genes of the exocytosis pathway. Indeed, the expression of several genes related to exocytosis and SNARE transport were altered in different Manf conditions (Table 8 Additional file 8). In Manf mutants, several genes implied in exocytosis and vesicle transport from Golgi complex to the plasma membrane were downregulated (Syx1A, Syx6, SNAP29), whereas the ER residing syntaxins - Stx17 and Stx18 - were upregulated. This supports an inhibition of secretion from Golgi complex to the plasma membrane as seen in ManfmzΔ96 mutant epidermal cells in vesicle accumulation close to the apical area (Figure 3C).
Additional file 8. Exocytosis and SNARE complex is altered in Manf mutants. A pdf file; an online coloured KEGG pathway showing altered gene expression in either red (upregulation), blue (downregulation), or in purple (altered gene expression) boxes. The unaltered known Drosophila homologues to identified components from other organisms are presented in green-filled boxes. The complete list of altered genes is summarised in Table 8.
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Table 8. List of genes with altered expression according to microarray analysis related to KEGG pathway of exocytosis and SNARE complexes
Expression of genes involved in cuticle development were altered in ManfmzΔ96 mutants
We have previously shown that ManfmzΔ96 embryos have disorganized cuticle [5]. At the end of embryogenesis from stage 16 onward, the cuticle components are secreted by epithelial cells and stored in regular layers, and subsequently the cuticular proteins are crosslinked by dopamine-derived quinones [29,30]. Among the downregulated genes in ManfmzΔ96 embryos, there were 14 genes coding the structural components of the insect cuticle. At the same time, several other genes responsible for cuticle development were upregulated, such as the genes encoding enzymes involved in chitin synthesis, krotzkopf verkehrt (kkv, chitin synthase-1) [31,32], knickkopf (knk, N-glycosylated membrane-bound extracellular protein involved in chitin microfibril formation) [33], and Syx1A (responsible for cuticle component secretion). Additionally, several genes involved in epithelial development and morphogenesis were upregulated and significantly enriched among the GO terms (35 genes) (Table 5 and Additional file 3).
We used transmission electron microscopy (TEM) analysis in ManfmzΔ96 mutants at the embryonic stage 17 to investigate the epithelial cells responsible for cuticle secretion. Indeed, these cells showed morphologically abnormal ER and accumulation of vesicles in the apical part (Figure 3A-C). It is possible that the enhanced endocytosis and disturbed exocytosis, together with misbalance in cuticular components, lead to disorganised and disrupted cuticle in ManfmzΔ96 mutant embryos. In larval ManfΔ96 mutant with gradually fading maternal contribution, the cuticle showed no disruption and the chitin layers were deposited and organised normally (Figure 3D, E). Instead there were problems in shedding the old cuticle and often the 1st instar cuticle remained attached (Figure 3E). This implies that the maternal loading of Manf gene products in larval ManfΔ96 mutants was sufficient to overcome defects in early cuticle development, secretion and layering, but insufficient to complete the first molt.
Large vesicles filled with electron dense debris are accumulated in ManfmzΔ96 mutant
To investigate the routes of membrane trafficking we evaluated genes involved in endocytosis. Of all Drosophila homologues known to be involved in endocytosis, 47% showed significant expression changes in our microarray experiment (Figure 4, Table 9). Genes coding for components of multivesicular body formation were especially altered. Several transmembrane receptors of growth factors were downregulated in Manf mutants and upregulated when Manf was overexpressed. PDGF- and VEGF-receptor related Pvr was upregulated in larvae in both lack and overexpression of Manf. Cbl, an E3 ubiquitin ligase and negative regulator of tyrosine kinase receptor signalling, was downregulated in mutant larvae and upregulated under Manf overexpression conditions. Two different members of endosomal recycling pathway, PAR family members and Rab-protein 11 (Rab11) were upregulated in mutants. PAR transcripts were upregulated by Manf overexpression as well.
Figure 4. Changes in gene expression related to endocytosis in Manf mutants. An online coloured KEGG pathway showing altered gene expression in red (upregulation), blue (downregulation), or purple (altered gene expression, differences between the two mutants) boxes. The unaltered components of the pathway with Drosophila homologues are coloured in green. White boxes represent the pathway members identified from other organisms with an unknown Drosophila homologue. The complete list of altered genes is summarised in Table 9. Notice the abundant expression changes in genes encoding proteins localised close to the plasma membrane and receptor tyrosine kinase internalisation pathway components. The changes of expression were highest among genes which products localise to multivesicular body and late endosomal compartments. Two branches of recycling pathway show upregulation of gene expression of key components PAR3, PAR6, and Rab11.
Table 9. List of genes with altered expression according to microarray analysis related to endocytosis KEGG pathway
To visualise Manf expression at subcellular level we used 3rd instar larval salivary gland cells that are the largest ones found in Drosophila. In the basal part, there was partial colocalisation of Manf expression with GFP-Rab11 (Figure 1Q-S) as well as with early endosomal marker GFP-Rab5 (Figure 1N-P). In larval fat body large cells with high secretory capacity GFP-clathrin light chain (Clc), a marker for clathrin coated vesicles, colocalised with Manf in some structures (Figure 1H-J). Manf localised close to GFP-Rab7, an important player in trafficking between the early and late endosomes and lysosomes, showing weak colocalisation (Figure 1L-N). Thus Manf localises to the endosomal structures with markers Clc, Rab5, Rab7, and Rab11; but probably does not share the same protein complexes with them.
Ultrastructural analysis of ManfmzΔ96 mutant stage 17 embryos revealed that the cells of secretory tissues such as gastric caeca, contain huge vesicles filled with cellular debris resembling multivesicular bodies and autophagosomes (Figure 3I). These structures were clearly missing in wild type embryos of the same age (Figure 3H). It is possible that these vesicles contain the misfolded proteins to be degraded or, alternatively, that the autophagy pathway is activated. The accumulation of vesicles full of debris to be degraded could be also due to the blockage in endosomal trafficking or lysosomal degradation.
Lysosomal genes are downregulated in Manf mutants
Because we detected in secretory cells the accumulation of multivesicular body like structures, is it possible that the lysosomal digestion mechanism was altered. Our microarray analysis revealed transcriptional change in 45% of lysosome related genes present in the KEGG database. Many of them were downregulated in ManfmzΔ96 embryos and some in ManfΔ96 larvae (Table 10; Additional file 9). The ATPase V-type H+ transporting subunit that maintains acidic environment in lysosomes showed downregulation in both mutants but was upregulated in Manf overexpressing larvae. The expression of other lysosomal membrane proteins and several lysosomal hydrolases was also altered.
Table 10. List of genes with altered expression according to microarray analysis related to KEGG lysosome pathway
Additional file 9. Lysosomal degradation is altered in Manf mutants. A pdf file; an online coloured KEGG pathway showing altered gene expression in either red (upregulation), blue (downregulation), or in purple (altered gene expression) boxes. The unaltered known Drosophila homologues to identified components from other organisms are presented in green-filled boxes. The complete list of altered genes is summarised in Table 10.
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At the subcellular level, Manf colocalises partially with ER-targeted marker and very poorly if not at all with the lysosomal compartment (Figure 1F-H). Nonetheless, it is possible that the lack of Manf modifies the fusion of lysosomes with multivesicular body-like structures by some still unidentified mechanism.
Paternal rescue of the ManfmzΔ96 mutant embryos leads to reduction in the amount of differentially expressed genes
In Drosophila, substantial bulk of transcribed mRNAs and translated proteins necessary for early embryonic patterning and development are maternally contributed to the oocyte at high levels. These mRNAs and proteins are stored and used gradually during the embryogenesis and in some cases through the whole larval development. When the embryonic lack of maternal Manf was rescued by paternal wild type gene expression, the transcriptome changes were evident but restricted to a smaller number of genes than changes caused by the complete lack of Manf. As many as 98 genes were significantly upregulated by paternal rescue resulting in 18 functionally enriched GO term clusters (Additional file 10). We obtained GO terms related to response to stimulus and neurological process (8 genes), consisting of genes like transcription factor pros, pumilio (pum; encoding a mRNA binding protein involved in nervous system development), pastrel (pst; with unknown molecular function involved in memory and learning), Rop, rl, and small optic lobes (sol; calpain family peptidase). Transcripts of several genes coding membrane proteins also showed enrichment. Among them, we observed many genes coding ion-binding proteins (19%) such as klumpfuss (klu) and odd skipped (odd; DNA binding Zn-finger proteins important for embryonic nervous system development). Other enriched GO term clusters were membrane (7 genes) together with cell division, cell cycle and cytoplasm (9 genes).
Additional file 10. DAVID functional annotation clustering analysis of upregulated genes in ManfmzΔ96 mutant paternal rescue. An Excel file; 98 genes were upregulated when maternal lack of Manf gene products was rescued paternally and grouped into 18 functional clusters.
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Only 34 genes were downregulated by paternal rescue giving 4 functional GO term clusters (Additional file 11).
Additional file 11. DAVID functional annotation clustering analysis of downregulated genes in ManfmzΔ96 mutant paternal rescue. An Excel file; only 34 genes were downregulated when maternal lack of Manf gene products was rescued paternally and grouped into 4 functional clusters.
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Ubiquitous overexpression of Manf results in transcriptional activation and upregulation of genes involved in cell cycle and cell death
We used enhancer trap line 69B-GAL4 to overexpress Manf, which we obtained as a commonly known GAL4 line with an epidermal expression pattern. After careful mapping the expression pattern of 69B-GAL4, we detected broad GAL4 expression in other tissues than epidermis - in central nervous system (non-glial), imaginal discs (wing and eye-antennal disc), garland cells, ring gland, but neither in fat body nor in gastric caeca. Ectopic expression of Manf under 69B-GAL4 rescues completely ManfΔ96 mutant lethality and the rescued adults are viable and fertile if maintained as a stock [5].
When comparing the gene expression profiles between Manf overexpressing and wild type larvae we found 614 genes upregulated that could be grouped in 102 functional GO term clusters (Additional file 12). This gene set showed enrichment in processes related to regulation of gene expression, protein localisation and transport, and cell cycle (e.g. kokopelli, an uncharacterized cyclin involved in stem cell maintenance and Retinoblastoma-family protein, the human Rb homolog). Genes involved in regulation of cell death were also upregulated (e.g. CG7188, a putative Bax inhibitor, rl, and klu). According to the previous study in HeLa cells, knockdown of MANF increased cell proliferation and susceptibility to ER stress induced cell death [7]. Our results support the involvement of Manf in regulation of cell cycle and cell death offering several candidate genes for further studies.
Additional file 12. DAVID functional annotation clustering analysis of upregulated genes in Manf ectopic overexpression by 69B-GAL4. An Excel file; 614 genes were upregulated and grouped into 102 functional clusters.
Format: XLSX Size: 125KB Download file
Manf overexpression in larvae caused downregulation of 340 genes annotated in 78 functional clusters (Additional file 13). The most prominent group consisted of GO terms such as membrane, plasma membrane, signal peptide, glycoprotein, disulfide bond, glycosylation site: N-linked (GlcNAc), integral to membrane, and transmembrane (77 genes). The majority of processes related to these GO terms take place in the ER such as cleavage of the signal peptide and disulfide bond formation. The main arthropod cuticular component chitin is composed of polymerised GlcNAc residues. Another prominent group was ion binding and metal binding (70 genes). Axon guidance, cell projection organization, neuron development, axonal defasciculation, cell motion, cell recognition (20 genes) were also enriched in line with our previous results implicating the role of Manf in neuritogenesis [5].
Additional file 13. DAVID functional annotation clustering analysis of downregulated genes in Manf ectopic overexpression by 69B-GAL4. An Excel file; 340 genes were downregulated and grouped into 78 functional clusters.
Format: XLSX Size: 157KB Download file
When comparing the upregulated genes in both paternally rescued embryos and in Manf overexpressing larvae, the common represented GO term clusters were ion binding (14 genes), membrane fraction (7 genes), oxidation reduction (8 genes) and cell cycle (5 genes) (Additional file 14). All together there were 57 annotated genes commonly upregulated by Manf, among these well known genes like Cbl, diaphanous (dia, formin, essential for actin-mediated events involving membrane invagination), Kinesin-like protein at 68D (Klp68D), rl, and Rop. Among the downregulated genes in both paternal rescue and Manf overexpression, there were only 6 genes in common e.g. CG34384, a diacylglycerol kinase involved in phosphoinositol signalling and glycerolipid metabolism. Conclusively, in Manf overexpression the typical growth factor signalling mediators rl and Cbl were upregulated. The links upstream of these mediators and downstream of secreted Manf still remain missing. The second cluster of genes was directly linked to membrane modifications and transport. Interestingly, the intracellular protein modification processes in ER were also enhanced by Manf overexpression. So presumably Manf has a dual role - one intracellularily in the ER, and the other extracellularly after being secreted.
Additional file 14. DAVID functional annotation clustering analysis of common upregulated genes in ManfmzΔ96 paternally rescued embryos and in Manf ectopically overexpressing larvae. An Excel file; 57 genes were upregulated in both Manf mutants and grouped into 9 functional clusters, 14 GO terms remained unclustered.
Format: XLSX Size: 19KB Download file
Differentially regulated genes in larval ManfΔ96 mutant in comparison to Manf overexpression
Next, we searched for genes showing downregulation in ManfΔ96 larval mutant and were upregulated in Manf overexpression condition, and vice versa. Altogether 89 probes on the microarray showed this opposite regulation resulting in 62 annotated Drosophila genes (Additional file 15). In this group, there were genes responsible for locomotory behaviour (3 genes), genes involved in oxidation reduction and metal binding (10 genes), in cell division (4 genes), genes coding membrane proteins (7 genes), and genes involved in insulin signalling (2 genes). Because of the diversity of GO terms and low number of genes the functional annotation clustering did not give statistically significant results.
Additional file 15. Complete list and DAVID functional annotation clustering analysis of genes downregulated in ManfΔ96 mutants and upregulated in Manf ectopic overexpression and vice versa. An Excel file; 46 genes were downregulated in larval ManfΔ96 mutants and upregulated in Manf ectopic overexpression, vice versa 16 genes were upregulated in ManfΔ96 mutants and downregulated in Manf ectopic overexpression. In the first sheet complete list of genes together with fold change logarithmic (FClog) results is presented. In the second sheet results of DAVID functional annotation clustering analysis with 9 clusters is shown.
Format: XLSX Size: 31KB Download file
UPR and Parkinson's disease
Among the known genes involved in PD, 32 are conserved between mammals and Drosophila, and 44% of these were differentially expressed in our microarray assay (Figure 5, Table 11). Importantly, several genes from dopamine uptake (Dopamine transporter, DAT) and synthesis (ple coding for TH, Ddc, and punch) were differentially expressed (Table 2, Additional File 1). The expression of many genes involved in mitochondria and ubiquitin proteasome pathways were also altered. Increasing evidence indicates that organelle stress is a key event in neurodegeneration [14]. UPR is an adaptive process aiming to restore the cell homeostasis under ER stress conditions and to re-establish the properly folded protein synthesis. Under irreversible ER damage UPR initiates cell death pathway to eliminate damaged cells. Manf could be responsible for the recovery and survival pathway in UPR. In this scenario, the lack of Manf especially in the secretory cells with high rates of protein synthesis, including neurons with intensive neurotransmission, might result in shift from ER stress to UPR towards cell death. However, the mechanism how Manf dispatches this function is still unclear. Recently the structural homology between the Manf C-terminal folding and SAP domain of Ku70 has been demonstrated [34]. SAP domain of Ku70 is known to inhibit Bax-induced apoptosis [35] and in vitro experiments with mammalian cultured neurons showed that Manf rescues the NGF-deprivation induced cell death as efficiently as Ku70 itself [34]. On the other hand we showed that the overexpression of Manf resulted in upregulation of genes involved in oxidation reduction. The DA neurons are known to be very sensitive to oxidative stress because dopamine metabolites are highly oxidative compounds [36]. Therefore the upregulation of genes responsible for oxidation reduction could be already protective for DA neurons. Besides reactive dopamine metabolites, the mitochondrial dysfunction has been implicated in the neurodegeneration occurring in PD. We found that, in ManfmzΔ96 mutants with degenerating DA neurites, the nuclear genes coding for mitochondrial proteins were significantly enriched among the downregulated genes.
Figure 5. Gene expression alterations in Manf mutants with a linkage to Parkinson's disease. A human disease KEGG pathway coloured with Drosophila homologues showing altered gene expression in Manf mutants - filled boxes with red represent genes that are upregulated, with blue downregulated expression or with purple differently altered gene expression between two Manf mutants. The unaltered components in the pathway are coloured green. Note that α-synuclein (SNCA) has no homologue in Drosophila. The complete list of altered genes is summarised in Table 11 with some additional genes not present in KEGG pathway but have been associated with PD elsewhere. The expression of genes with a role in dopamine uptake, intracellular transport, and synthesis is altered. Mitochondrial oxidative pathway complex I encoding genes show upregulation. The other mitochondrial proteins encoded by nuclear genes involved in PD such as Htra2 and DJ-1 expression is downregulated in Manf mutants. Some members of ubiquitin pathway are altered as well.
Table 11. List of genes with altered expression according to microarray analysis related to KEGG pathway of Parkinson's disease
We have studied the gene expression changes of two slightly different Manf mutants in Drosophila. Surprisingly, the expression profiles of embryonic lethal ManfmzΔ96 and larval lethal ManfΔ96 mutants were quite diverse. It might be due to the dissimilar roles of Manf gene during embryonic and larval stages or it indicates the difference between the absolute lack of Manf versus gradual diminishing of maternally contributed stores of Manf gene products. Our microarray analysis followed by functional annotation clustering revealed statistically significant enrichment related to metabolism and membrane transport and transporters. The observed changes in membrane traffic were supported by ultrastructural studies of ManfmzΔ96 mutant. More than 40% of known Drosophila genes related to ER and UPR showed altered expression levels in Manf mutants. We found changed expression of several genes known to be associated with processes altered in PD such as oxidative phosphorylation, mitochondrial function, dopamine metabolism and uptake, and protein ubiquitinaton. The lack of Manf in ManfmzΔ96 mutant embryos resulted in massive upregulation of stress, defense, and immune response related genes as well as genes involved in proteolysis and cell death. Overexpression of Manf resulted in upregulation of genes involved in oxidation reduction, an important process to protect DA neurons from oxidative stress. Thus, our results support the previously reported findings in mammalian cells that upregulation of Manf is important in UPR and could be protective for the cell. It is also evident that ER stress leads to UPR and cell death in the absence of Manf. These effects were less drastic when Manf was gradually abolished in ManfΔ96 mutant larvae and related to several metabolic processes and downregulation of genes involved in replication, transcription and splicing. Still, stress and defense related genes were enriched among the upregulated genes of both Manf mutants.
Fly strains
Drosophila melanogaster adults were maintained at 25°C on malt and wholemeal flour based standard food, for egg laying apple juice agar plates with yeast paste were used. Wild type flies were w-, all genotypes used were of w- background: hs70Flp/+;; FRT82BManfΔ96/ManfΔ96, ManfΔ96/TM3 Sb Ser twi > GFP. For ectopic overexpression 69B-GAL4 [37] and UAS-DmManf133 (3rd chromosome insertion) were used. As an ER marker ER-targeted sqh-EYFP flies were used. UAS-GFP-rab5, UAS-GFP-rab11, UAS-chc-GFP, and UAS-GFP-rab7 were obtained from C. Samakovlis and ppl > GAL4 from V. Hietakangas. Other fly lines were obtained from Bloomington Drosophila Stock Center or generated by us [5].
RNA isolation
For RNA extraction, embryos were collected from apple juice plates, washed with embryowash, dechorionated by standard bleach treatment and washed thoroughly. Both embryos and larvae were separated and picked by phenotype and staged under microscope onto fresh apple juice plates, collected into Eppendorf tubes and snap frozen. Total RNA was extracted with Qiagene RNAeasy extraction Kit (Qiagene) according to manufacturer's recommendations, treated with RNase-free DNase (Promega) 15 min at 37°C and purified by RNA clean-up kit (Macherey-Nagel). RNA was quantified using a NanoDrop-1000 spectrophotometer and RNA quality was monitored by the Agilent 2100 Bioanalyzer (Agilent Technologies).
Microarray experiments
For microarray experiment three biologically independent samples for each genotype were used. Diluted spike controls (Agilent) were added to 1 μg of total RNA samples and in vitro transcribed and labeled with Amino Allyl MessageAmp™ II aRNA Amplification Kit (Ambion/Applied Biosystems). The dyes used were cyanine 3 (Cy3), cyanine 5 (Cy5) or Alexa 488, as previously described [38]. Dye incorporation and received aaRNA yield were monitored by the NanoDrop ND-1000 Spectrophotometer.
5 μg of each differentially labelled aaRNA was fragmented at 60°C for 30 min in a reaction volume of 55 μl containing Agilent fragmentation buffer and 2x Agilent blocking agent following the manufacturer's instructions. On completion of the fragmentation reaction, 55 μl of 2x Agilent hybridization buffer was added to the fragmentation mixture and hybridized to Agilent's fruit fly Microarray Kit 4x44k, P/N G2519F (Agilent Microarray Design ID 018972) for 17 hours at 65°C in a rotating Agilent hybridization oven. After hybridization, microarrays were washed 1 min at room temperature with GE Wash Buffer 1 (Agilent) and 1 min with 37°C GE Wash buffer 2 (Agilent), then dried immediately by brief centrifugation. The slides were then scanned by Axon 4200AL scanner.
DNA microarray analysis
The microarrays images were segmented and the median intensity of each spot was estimated by the software GenePixPro® 6.0 (Axon). The data were then imported into R software http://cran.r-project.org/ webcite and preprocessed by the BioConductor package Limma [39]. Linear model followed by moderated t-test was utilized for finding the differentially expressed genes (P-value < 0.01 after Benjamini and Hochberg correction) between ManfΔ96, ManfmzΔ96, ManfmΔ96/+, 69B > Manf133 and w-. Lists of significant genes were screened by the DAVID 6.7 annotation tools [40,41] in order to find over-represented biological themes. Default DAVID parameters were used. To identify the pathways altered, the online tool available from Kanehisa laboratories, KEGG Mapper was used [42]. All microarray data are MIAME compliant and available at the NCBI GEO database (ID GSE28820).
Quantitative PCR
For qPCR, independent biological samples were used for RNA extraction. 4 μg of total RNA was reverse transcribed with MMLV reverse transcriptase (Promega) according to manufacturer's instructions using oligo(dT) primers. The primers were designed with the help of Universal ProbeLibrary Assay Design System (Roche Applied Science) and are listed in Additional file 16. qPCR was performed with Lightcycler 480 real-time PCR system (Roche Diagnostics) with the help of pipeting robot Robotics4 (Corbett Robotics) on 384-well plates using Lightcycler 480 SYBR Green I Master complemented with 5 pmol of primers and cDNA corresponding to 40 ng of total RNA used in reverse transcription. Three replicates for each reaction were included in the PCR runs. Results were analysed with Lightcycler software version 18.104.22.168.
Additional file 16. List of all designed primers used for qPCR.
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Transmission electron microscopy and immunohistochemistry
The embryos for TEM were treated as previously described [43]. The whole larvae were subjected to high pressure freezing to visualise the cuticle layers as described earlier [44]. The primary antibodies used were rabbit phospho-eIF2α (Ser51) antibody (1:1000, Cell Signaling Technology), mouse α-DmTubulin (1:1000, Sigma) and rabbit α-DmManf (1:1000) [5]. Immunohistochemistry and imaging were performed as previously described [5]. To visualise the lysosomes, Lysotracker Red DND99 (Invitrogen) was used. The red colour of Alexa568 dye was changed to magenta in order to help colour blind people to distinguish it in the combinations with green.
Western blotting
For Western blotting about 100 embryos of stage 17 were collected, genotyped, and homogenised in 10 mM HEPES, 1 mM EDTA, 0.25 M sucrose homogenising buffer, pH = 7.3 in the presence of protease inhibitor cocktail (Complete Mini, Roche). The concentration of proteins was measured with Bio Rad protein assay DC reagents. The equal amounts of total protein were mixed with 3 × Laemmli loading buffer and boiled at 99°C for five minutes. Up to 6 μg of total protein were loaded per lane to SDS-acrylamide gel. Western blotting was further proceeded according to the standard manufacturer's instructions (Amersham Biosciences). For the quantification of Western blotting results ImageJ analysis software was used. Quantification was based on area measurements and intensity calculations in comparison with the anti-tubulin loading control.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
MP, TIH, DG, and PA designed the research, MP performed the laboratory experiments, DG scanned the slides and made the analysis and comparisons of the raw scanned microarray data, MP with the help of RL completed the annotation, and MP made functional annotation clustering analysis, all other laboratory experiments and wrote the draft manuscript. All authors read, made corrections and approved the manuscript.
We thank Mart Saarma, Päivi Lindholm, Osamu Shimmi, Christos Samakovlis and Ville Hietakangas for reagents and fly stocks, Eeva-Marja Turkki for the technical assistance, Jouni Kvist, Panu Somervuo, Petri Törönen, and Ilya Plyusnin for the comments and help with microarray analysis, Mikko Airavaara for the help with Western blot quantifications, Juha Partanen and Johan Peränen for critical comments during the writing of the manuscript. This work was supported by the Academy of Finland Neuroscience Program, the Michael J. Fox Foundation, the Finnish Parkinson Foundation and the University of Helsinki research funds.
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Swing Keel or Fixed Wing Keel? Catalina 22 or 25?
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Swing Keel or Fixed Wing Keel? Catalina 22 or 25?
Hello all,
I'm brand new to the board, and to sailing. For some reason, the sailing bug has bit (though to be honest, I have wanted to do this since I was a teenager). I am beginning to look into a small pocket cruiser type of boat to sail on inland lakes, and/or Sandusky Bay/Lake Eire. I live in the Cincinnati area.
I have been looking at the Catalina 22 and Catalina 25 more and more. I have eliminated the MacGregor 26X, and the Hunter water ballasted boats from my list of possibles. What I am really looking for is a trailerable boat that can be launched and retrieved relatively easily. What would be better, a swing keel, or a fixed wing keel for ease of trailering (launching and retrieving)? Both the C22 and C25 have a swing keel option, or a wing keel option. Or, are they both equally trailerable?
Next question: Which is the better boat for day sailing and short multi-day trips, the C22 or C25 (given the trailering requirement above)?
Thanks for your help.
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Surfline Surf Forecasts
Pleasure Point
Cheap money is gone …did you miss the boat or can you recover?
We are done with low interest rates. Did you know that ALL CASH real estate purchases are up in the last year to 50% in some sectors of the bay area real estate market? Read why here
Some of the perks of owning your own home
What you might now have realized
Can you afford to own a home?
I'm not talking about mortgage payment here or PITI/ principal, interest taxes and insurance. Closest article that I could find was from the Bureau of Statistics (they're safe) and needs to be extrapolated to 2011 costs. What they don't talk about is that home ownership keeps you home more so gas prices of going places, […]
3.94% for 30 year mortgage!
Lowest rates in 30 years Read the story and see the retrospective Look for homes now:
Why buy a retirement home when you're working?
Deciding to buy a retirement home while you are still working is a decision that is based on a long term projection of your future income and plans. Here are some of the reasons to buy your final retirement home now. 1. You will more easily be able to qualify for the loan while you […]
Should I Buy or Sell in 2013?
Make wise decisions regarding your most important investment: Your home. Follow these basic 5 rules if you are thinking of buying or selling: Create a clear financial picture of your ability to buy or continue to live in your home based on income and cost of living increases. "Carpe Diem:" If your dream home is […]
Kale and Lentil Soup
This hearty soup uses kale, a wonderful winter vegetable with a tremendous vitamin content. Guaranteed to keep your family warm and fueled. Ingredients 2 Tbs olive oil 1.heat oil:cook onions and garlic till translucent 2 yellow onions 4 garlic cloves 1 pound mild Italian sausage (optional) 2. Brown over medium heat in a skillet and […]
Community: An Allegory
Living together in a neighborhood defines security, comfort and ultimately health and happiness. Realtors should be here to help you find your community. Keiki is a 6'4″ gorilla with a bad temper. Sounds like a lot of people these days after the first wave of the recession. Smart people, like gorillas, found sanctuaries in their […]


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Science News Archive - April 07, 2011
Global Heating Element Provider Acquires Open Coil Heating Element Technology Franklin, WI (PRWEB) April 6, 2011 Zoppas Industries, a global leading manufacturer of heating element technologies, today announced the acquisition of Nova Industries, a leading manufacturer of open coil heating elements.
Gary Egelhoff (Big K Energy-Field Operations Manager) and Jerry Higgins (Big K energy-Completion Consultant) have taken up temporary residency on the Big K Energy 320 acre lease in Scurry-Mitchell Counties.
A new form of video self-modeling therapy for children with autism is being introduced by way of an app called Ther-Ad for Autism (Therapeutic Advertisements for Autism), helping to recognize April as National Autism Awareness Month. Wilkes Barre, PA (PRWEB) April 6, 2011 A new form of video self-modeling therapy for children with autism is being introduced by way of an app called Ther-Ad for Autism (Therapeutic Advertisements for Autism), helping to recognize April as National Autism Awareness Month. The new app was developed by a team at Data Makes the Difference and mHealth researcher Dr.
April is National Donate Life Month. Philadelphia, PA (Vocus/PRWEB) April 06, 2011 Did you know there are currently 1,988 kids in the United States on the transplant wait list, in need of a new organ to survive? Carol O'Brien and her son, Riley, know this startling reality all too well.
Many companies are creating new and highly interactive websites with phenomenal visual appeal. However, overlooking the importance of content management and migration can lead to delays in the project launch and increase costs significantly.
Innovative new press release service mixes old school PR writing with leading edge social media tactics to deliver better media coverage and visibility for companies and organizations. Arlington, VA (PRWEB) April 6, 2011 A new and innovative press release writing and distribution service optimized for the Internet that will give businesses and organizations an advantage by delivering better media coverage and visibility was launched today. The new service, 21stcenturypressrelease.com, is designed to help companies and organizations reach the media and customers more effectively by writing and distributing news releases that couple old fashion professional PR writing techniques with modern social media tactics that is essential for success. "A press release is no longer just a press release,†Ken Jaques, president of Global Communications Strategies and founder of 21stcenturypressrelease.com said.
Taxonomy provides a jump start for companies who are looking to leverage the taxonomy and managed keyword capabilities in Microsoft SharePoint 2010 Denver, CO (PRWEB) April 6, 2011 WAND, Inc.
Colo@ launches new website. Provides detailed information on facilities and services for customers and potential customers. Atlanta, GA (PRWEB) April 6, 2011 Colo@ (http://www.coloat.com), a world leader in colocation services, today announced its new website.
Online tutorial gives researchers and scientists a place to learn about the many biology resources available to them.
European Sperm Bank USA now offering a chance to discuss donor options via Skype. Seattle, WA (PRWEB) April 6, 2011 European Sperm Bank USA (ESB-USA), the leading U.S.
- A young chicken: also used as a pet name for children.


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Boat and marine steering wheels and controls. Gander Mountain has a large selection of replacement boat steering wheels and controls. We carry boat steering wheels, boat steering knobs, boat steering cables, boat controls and control cables. When having to replace your boat steering or boat controls, Gander Mountain carries the boat steering and control products you need to get the job done!
SeaStar Solutions No Feedback Twin Cable Rack-and-Pinion Steering...
SeaStar Solutions model: SS152
Available lengths: 12 - 16
4 turns lock-to-lock
Uflex Single Lever Top-Mount Control
Operates both throttle and shift
Built-in safety features
Made from marine grade aluminum
SeaStar Solutions No Feedback Single Cable Rack-And-Pinion ...
SeaStar Solutions model: SS151
Available lengths: 10 - 20
4 turns lock-to-lock
SeaStar Solutions Hydraulic Steering Fluid
Use with SeaStar and BayStar Steering Systems
SeaStar Solutions Safe-T Quick-Connect Rotary Steering System, ...
SeaStar Solutions model: SS137
SeaStar Solutions The Rack Back-Mount Rack-and-Pinion Steering ...
SeaStar Solutions model: SS141
Available lengths: 9 - 18
4 turns lock-to-lock
Standard steering system
For use with power-assisted O/B, Sterndrives, I/B
Panther Electro Steer Wireless Remote
Features a 50 range
Operates on 12V battery
SeaStar Solutions Viper Steering Wheel
UV-resistant, non-corroding marine grade materials
Fits standard 3/4" tapered shafts
14" dia.
SeaStar Solutions Ace Steering Wheel
Rugged, stylish design.
13" diameter.
Fits standard 3/4" tapered shafts.
Schmitt Destroyer Steering Wheel With Polished Grip, 13.5" ...
Heavy-gauge stainless steel
Cast aluminum hub
T-H Marine Zippered Rigging Sleeve 48"
New! SeaStar Pro Hydraulic Steering Kit Without Hose
Precise steering control
Best for single-engine outboard boats
Easy to install
Meets ABYC and NMMA requirements
SeaStar Hydraulic Steering Kit With Hose
Easily installed steering system
Fits most single/twin outboards
Smooth and efficient control
3/4" steering wheel shaft
Hose with 1,000 PSI capacity
New! SeaStar Hydraulic Steering Kit Without Hose
Easily installed steering system
Fits most single/twin outboards
Smooth and efficient control
3/4" steering wheel shaft
SeaStar Pro Hydraulic Steering Kit With Hose
Precise steering control
Best for single-engine outboard boats
Easy to install
Meets ABYC and NMMA requirements
Detmar Daytona Steering Wheel
Hard grip with center cap
13" diameter
SeaStar Solutions NFB w/Tilt Helm Rotary Steering System, SS157
SeaStar Solutions model: SS157
4.2 Tilt Cable Steering Kit
Available in 11 - 20 lengths
UFlex HYTECH 1.0 Hydraulic Steering Kit, Up To 150 HP
Easy and affordable steering package
For boats with less than 150 HP
Compatible with most 2 and 4-stroke engines
Includes 40 tubing
UFlex Side-Mount Control with Trim Switch, chrome
Power trim switch located in handle grip
Pull-out handle disengagees shift
Brake tension release system outside adjustable
Positive lock-in neutral
Exceeds EN ISO 11547 standards
SeaStar Front-Mount Hydraulic Cylinder - HC5358 Cylinder
Direct replacement cylinders
For most original equipment hydraulic systems
Fits all applications listed below


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StartUpHire is the place to find Production Database Administrator jobs. Review the list of Production Database Administrator career opportunities to the left. These Production Database Administrator jobs represent some of the most rewarding careers available.
You can refine your job search for Production Database Administrator career opportunities by adding a job title, company name, or keyword to the search box above … or click the "Advanced Search" link for more options.
Don't see the employment opportunity you're looking for? Check back soon for more Production Database Administrator jobs, or sign up for an email alert (link below) to receive periodic updates on new Production Database Administrator jobs.
The Senior Database Administrator is responsible for participating in building, operating, and maintaining a heterogeneous datastore environment in the Technical Operations group. The data environment consists primarily of MySQL, PostgreSQL, Vertica and A5/24/2015 New York, NY Save This Job
Overview: The position for a Sr. Database Administrator (DBA) / Architect combines the efforts of design, implementation and administration of ORACLE, MS SQL and MySQL database environments. This position will report to the VP of Infrastructure Engineering and will be focused on5/17/2015 Austin, TX Save This Job
PERFORMANCE PROFILE: The Senior Database Administrator will concentrate on designing, implementing, managing and supporting production and QA database systems including Oracle, MySQL and SQL Server. Candidates for this position should enjoy: Working closely with Boingo teams4/27/2015 Los Angeles, CA Save This Job
Engineering Boston, MA, United States Zipcar, the world?s largest car-sharing company, is looking to add new talent to our team. Today, Zipcar provides hundreds of thousands of people with a viable alternative to traditional car ownership, saving them money, time and5/13/2015 Boston, MA Save This Job
OG-NET, Inc. is seeking a Production Application Administrator to join its team! As a Production Application Administrator, you will be responsible for administrating high availability systems in a fast paced environment. This includes installing, configuring, testing, and4/25/2015 Red Bank, NJ Save This Job
Job Tracking ID: 85373-190526 Location: Birmingham, AL Job Type: Full-Time/Regular Date Updated: April 21, 2015 Job Description: The Junior Database Administrator?s role is to design, install, monitor, maintain, and performance tune production databases while ensuring5/19/2015 Birmingham, AL Save This Job
Summary: We are looking for a Lead Database Administrator with extensive project management experience who has experience with various technologies including Oracle RAC, GoldenGate, OBIEE and overall database design and architecture. Key responsibilities: Database design,4/25/2015 San Jose, CA Save This Job
About Bullhorn We build applications that put the world to work. Headquartered in Boston with offices in London, Sydney, St. Louis, and Vancouver, Bullhorn is the global leader in recruiting software. Our love of technology, appreciation for customers, and respect for each other5/4/2015 Boston, MA Save This Job
Requisition Number15-0059 Post Date4/23/2015 TitleDatabase Administrator (A07) CityLouisville StateKY DescriptionSummary Plans computerized databases, including base definition, structure, documentation, long-range requirements, operational guidelines and protection. Ensures5/26/2015 Louisville, UT Save This Job
Category: Domestic Minimum Experience: 0-2 years Required Education: Bachelors Degree Job Description In this role, the DBA will work closely with members of the development team as well as the database teams responsible for integrating our AGI software products with Oracle4/18/2015 Exton, PA Save This Job
This position is a part of Technology Operations department providing database administration and support for our production and development databases to ensure a highly scalable and reliable database environment for web-based applications. RESPONSIBILITIES: As of part of the4/11/2015 Emeryville, CA Save This Job
PowerReviews delivers software that more than 1,000 brands and retailers utilize to collect, display, and syndicate reviews on more than 5,000 websites. An essential resource for consumers as they search and shop online and in-store, reviews drive traffic, increase sales, and5/8/2015 Chicago, IL Save This Job
Job Description As a member of the DBA team, you will be responsible for configuring, managing, maintaining and monitoring SQL Servers in our Production environment. Our DBAs will also assist in supporting our SQL Servers in multiple Development environments. Database security,4/28/2015 San Clemente, CA Save This Job
JOB PURPOSE SUMMARY As a member of the IT Operations DBA team, this individual will be responsible for configuring, managing, maintaining and monitoring SQL Servers in our Production environment. This individual will also assist in supporting our SQL Servers in multiple4/28/2015 Draper, UT Save This Job
You are a database administrator with strong foundations supporting SQL Server in a 24/7 production environment. You are looking for an opportunity to delve deeper into and expand your knowledge of heterogeneous database environments. Webroot is seeking to provide a database3/30/2015 Broomfield, CO Save This Job
Job ID 2014-2239 Location US-NC-Morrisville Category Information Technology Type Regular Full-Time More information about this job: Overview: SciQuest is the largest publicly-held provider of Software-as-a-Service (SaaS) procure-to-pay technology. We?re on a Quest to develop3/24/2015 Morrisville, NC Save This Job
Job ID 2015-2359 Location US-PA-Pittsburgh Category Information Technology Type Regular Full-Time More information about this job: Overview: SciQuest is the largest publicly-held provider of Software-as-a-Service (SaaS) procure-to-pay technology. We?re on a Quest to develop4/23/2015 Pittsburgh, PA Save This Job
Who We Are and What We Are Looking For? Your Big Three Ways You?ll Help Small Businesses Succeed: Improve Production Operations database infrastructure to drive platform strategy Improve database performance to increase product NPS Enable confident monthly releases through3/24/2015 Chandler, AZ Save This Job
Austin, TX Development About us: Revionics is a proven leader in End-to-End Merchandise Optimization solutions. Over 40,000+ retail locations around the world optimize with Revionics across 18M+ products and 2.2B+ sku/store combinations are modeled weekly. Revionics empowers5/18/2015 Austin, TX Save This Job
This is a Senior MSSQL Server Database Administrator role largely responsible for providing operational database services to the organization. Some of the primary responsibilities of this role include: owning, tracking and resolving database related incidents and requests,5/26/2015 San Luis Obispo, CA Save This Job


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Former Liberal Democrat leader Charles Kennedy dies aged 55
Video: Former Liberal Democrat leader Charles Kennedy has died aged 55 at his home, his family said.
09 May 2014
In newspapers over the past decade a two-word phrase, usually accompanied by a sigh, has emerged as an explanation for continual and controversial changes — "digital disruption".
22 January 2014
Savoy Theatre: 22 January – 7 February 2014
21 January 2014
David Blaine is back, but there will be no more starvation stunts or ice baths. He tells Jane Mulkerrins about working his magic on celebrities and why being a father means he now has some new tricks up his sleeve
23 December 2013
Thousands of Christmas presents are being delivered to sick, orphaned and disadvantaged young Londoners by an army of volunteers in reflective jackets and sunglasses.
17 December 2013
An 'alien' creature filmed swimming in Bristol harbour has been revealed as a hoax by television producers who created it for a new series about illusions.
06 December 2013
Sir John Major has criticised the "truly shocking" dominance of the upper echelons of power in Britain by the privately educated and affluent middle class, it was reported.
11 November 2013
Arsenal have produced some magic in the early part of the season to take them to the Premier League table summit, but the club's players were treated to something out of the ordinary when magician Drummond Money-Coutts made a visit to the Gunners' training ground.
15 October 2013
It's amazing the transformation that can be made with a touch of makeup and new clothes.
08 October 2013
28 September 2013


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Reorganizing Kitchen Cabinets and Drawers
Real Simple made over one woman's cluttered kitchen.
Before the Makeover
Beth Jorgensen really tries to be organized. The 44-year-old mother of two from Fargo, North Dakota, decided to reorganize all her packed kitchen drawers, with mixed results. She opted to store the family's drinking glasses and stemware in an adjacent cabinet―on a lazy Susan. A disaster waiting to happen with every spin.
And there were more problems. Despite Beth's organizational attempts, each cabinet overflowed with items grouped with no rhyme and little reason. Antique china and glass serving bowls were stacked on high shelves―another opportunity for accidents. Then there was the clutter: spice containers tucked into every spare inch, special-occasion candlesticks crammed into prime real estate, not to mention the food-container drawer, where anything and everything jockeyed for space with the hand-mixer and the lidless cottage-cheese tubs.


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Green Mustaches
School Color Moustache Green
In Stock - 1 business day processing+shipping
Your Price: $1.29 each
Minimum Order Quantity: 1
Green Mustaches are a hilarious way to show your school pride!
Our self-adhesive Green Mustaches look like a classic twirl mustache. Each Green Mustache is made of felt and measures 5 1/2 inches long x 2 3/8 inches high. Sold individually. Have your friends join you and you can all wear a Green Mustache to your next game.
Please allow ample time for delivery. Our ship date for this product is noted in your shopping cart. Many items are shipped separately, so please allow extra time for these items to arrive. Shipping charges are based on the value of the merchandise and not the number of shipments. For additional shipping information, contact our Customer Service Department at (firstname.lastname@example.com or 1-800-314-8794).
Detailed shipping information
Detailed shipping information
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Questions & Answers
Do you have questions about this product?(earn points for answers!)
get answers from real customers and in-house experts with AnswerBox.
1 Question | 1 Answer
Displaying question 1
Q:Do these have a sticky back?Asked on 9/14/2014 by Renee
1 answer
A:yes they doAnswered on 9/14/2014 by csr
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You can now invite anyone to meet at your favorite Starbucks.
Limited-time feature
30 year old woman | Jacksonville, FL, USA | Seeking men 23-34 within 50 miles
As honest as a photobooth
I'm the girl next door with an edge. I don't mind getting dirty when it calls for it. I have a fun loving sarcastic sense of humor. My honesty scares me. I don't wear my heart on my sleeve. I am PROUD of my Hawaiian roots! I volunteer as often as I can. Dance is my first love! I take photos to capture moments and perspectives not everyone takes the time to notice. My alcohol tolerance is higher than ALL girls and MOST guys I know, thanks to my brothers. This does not mean I'm a party girl it means I was taught not to be a lush, taken advantage of, or one to be babysitted.There is almost always a hair band on my right wrist. Road trips are MY FAVORITE!! I founded "Estrogen Night" and "Fat Kid Day." I am looking to be someone's someone not someone's sometimes. I want to cuddle and watch a movie, take a roadtrip for no reason, fall asleep to someones heartbeat, run my fingers through his hair until he falls asleep and not care that he's snoring through the best part of the movie. I want to make out and not just blah blah blah here's a kiss goodnight. I want his hand on the small of my back pulling me closer into him. I want to put my hands gently on the side of his face as our lips meet to return the endearing gesture. I want to drink beer with someone and eat an insane amount of barbecue while yelling at the tv screen because the ref made a bad call. An when I talk about my brackets he knows its basketball. Hell I want to sit in pj's and bulsh*t about absolutely nothing and laugh until our cheeks hurt cause we are just that random. I'm not your average girl I have morals and values that I cherish. I love to get dressed up but you can give me a tshirt, baseball cap, torn jeans and flip flops anyday.I believe in Southern Hospitality...opening the door, yes mam, no sir. I am a Romantic at Heart, Optomisticly Minded, and a Realist by Nature. You've read this far don't hesitate to send me a message :).
Camping , Coffee and conversation , Dining out , Movies/Videos , Museums and art , Music and concerts , Nightclubs/Dancing , Performing arts , Playing sports , Travel/Sightseeing , Volunteering , Watching sports , Wine tasting
Aerobics, Bowling, Cycling, Dancing, Football, Other types of exercise, Running, Skiing, Swimming, VolleyBall, Walking / Hiking, Yoga
I exercise 3-4 times per week.
I have Dogs
I like Horses
Middle of the Road
Z{{Cta}} {{#if HasMoreInfo}} ?{{CtaSecondary}} {{/if}}
|What she is looking for
|5'7" (170cms)
|5'10" (177cms) to 6'6" (198cms)
|Body type:
|Athletic and toned
|Slender, About average, Athletic and toned
|No preference
|Light brown
|No preference
|No Way
|No Way, Occasionally, Yes, but trying to quit
|Social Drinker
|No preference
|Artistic / Creative / Performance
|No preference
I am a photographer and I teach dance
|I'll tell you later
|No preference
|Never Married
|Never Married
|Have kids:
|Wants kids:
|Pacific Islander, White / Caucasian, Other
|Latino / Hispanic, Native American, Pacific Islander, White / Caucasian
|I'll tell you later
|Christian / Catholic, Christian / LDS, Christian / Protestant, Spiritual but not religious, Christian / Other
|English, Other
|No preference
|Some college
|Some college, Associates degree, Bachelors degree, PhD / Post Doctoral


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Originally written on MyTeamRivals.com | Last updated 11/15/14
Against the Padres Saturday night, Roy Halladay put forth another great effort. Through 7 innings, he allowed only 2 runs while working in and out of trouble. This was the Halladay we are all used to seeing. Unfortunately, the Phillies offense was also the one we are all used to seeing recently. John Mayberry Jr. and Freddy Galvis teamed up in the 7th to score the Phillies only run. Shane Victorino had 3 hits and a walk, but little to show for it as he kept getting stranded on base. The Phillies loaded the bases a number of times in this game and got nothing out of it. Hunter Pence was one of the worst offenders; he struck out once and popped up when there was only 1 out in the inning and a sac fly would have scored a run. Ty Wigginton was just as bad. He came up to bat twice with the bases loaded; he flew out once and grounded out once. Pence, who hit .314 last year, has been awful in 2012. When asked about it, Charlie Manuel told reporters, "Now, what we do about it, I don't know....
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The Town & Country Inn consists of two buildings: south (Town) and north (Country). Learn more about the features and amenities of both buildings.
Breakfast room 'hostess' very helpful and friendly. Very knowledgeable about the hotel. J.
This room, located on the north side of 200th street, places you right across from the city park, our annual antique mall, Depot Pizza, RV plaza and the Depot Junction C-Store.
Ph (435) 586.9900
(800) 493.0062
fax (435) 586.1664
© 2002-2013 Best Western International, Inc. All rights reserved.
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