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May 28, 2015
At Behind the Bench we've highlighted a few applications using Sanger Sequencing, including checking health supplements with the Consortium for the Barcode of Life project, applying Short Tandem Repeat fragment analysis by capillary electrophoresis to cheetah conservation efforts and easily verifying variants discovered using Ion AmpliSeq™ technology. Here we'll give a broad overview of common Sanger sequencing by capillary electrophoresis applications.
May 26, 2015
Recently the NIH Roadmap Epigenomics Consortium published a set of 24 papers, the
primary one examining over a hundred reference epigenomes from a broad range of cell lines and tissues. What is meant by epigenomics?
What are epigenomic factors?
Epigenomic factors include DNA methylation, histone modification (both methylation and acetylation), and non-coding RNA expression, the three of which all affect gene expression.
May 22, 2015
Go Digital PCR: Pt 5 – Rare Mutations is the fifth segment in our six-part series introducing you to digital PCR (dPCR) and how it is capable of detecting and quantifying rare mutations for low-prevalence targets in cancer research samples.
Rare mutation detection is a powerful tool in cancer research
In oncologic research, rare mutation detection among a large population of wild type cells poses a significant challenge. The accumulation of mutations in oncogenes or tumor suppressor genes is an important aspect of tumor formation.
May 18, 2015
How does the Italian National Police use QuantiFiler™ Trio*?
We caught up with Stefano Vernarecci from the Forensics Genetics Laboratory of Rome at the Madrid Human Identification Solutions Conference (HIDS) to learn how does the Italian National Police use QuantiFiler™ Trio in their casework. Stefano was keen to share the value of the Degradation Index feature in the Trio that can help save time, money & DNA while processing challenging casework samples.
May 15, 2015
Very good databases for reference samples & highly sensitive Y-STR chemistries such as Applied Biosystem YFiler® Plus could help close many sexual assault cases.
Dr. Lutz Roewer explains that we must avoid closing a case just because we do not see anything on the autosomal analysis since a dual workflow, using both autosomal and Y-chromosome analysis can help provide full information.
May 14, 2015
Reading about research is one thing, but nothing beats seeing it for yourself in person. So when we had the opportunity to with meet the people behind the Cheetah Conservation Fund (CCF) and learn more about how sequencing is impacting their conservation efforts, we jumped at it – and how often do you get a chance to see a real Cheetah up-close?
It's no secret that the cheetah has suffered tragic setbacks at the hands of human-wildlife conflict, and according to CCF, their population declined by 90% in the 20th century.
May 12, 2015
Very good databases for reference samples & highly sensitive Y-STR chemistries such as Applied Biosystem YFiler® Plus could help close many sexual assault cases.
Dr. Lutz Roewer explains that we must avoid closing a case just because we do not see anything on the autosomal analysis since a dual workflow, using both autosomal and Y-chromosome analysis can help provide full information.
May 8, 2015
Go Digital PCR: Pt 4 – NGS Libraries is the fourth segment in our six-part series introducing you to digital PCR (dPCR) and now, how it enables highly precise next-generation sequencing (NGS) library quantification.
Simple, accurate, and affordable NGS library quantification with dPCR
dPCR is a simple and accurate technique for quantifying NGS libraries that helps avoid the potential miscalculation of library concentrations that may occur when using other techniques, such as...
May 5, 2015
At the Association for Biomolecular Resource Facilities conference in Saint Louis Missouri USA, Mike Lelivelt described the Thermo Fisher TM Cloud analysis capability with not only real-time PCR data but also Sanger Capillary Electrophoresis sequencing data.
Mike Lelivelt (Thermo Fisher Scientific, San Francisco California USA) shared a talk entitled "Integrating the Analysis of Nucleic Acid Data across Multiple Platforms on the Cloud". We caught up with Mike to discuss his presentation and what the Thermo Fisher TM Cloud has to offer.
May 5, 2015
Part 3 of our series on Sanger Sequencing by Capillary Electrophoresis is about primer design. Primer design for Sanger sequencing is a very straightforward process with online tools such as the Primer Designer™ Tool which offers >300,000 primer pairs targeting the human Exome as well as the OligoPerfect™ Designer But what about primer design for more specialized Sanger CE applications, such as for human identification?
I interviewed Julio Mulero, a senior staff scientist with Thermo Fisher Scientific whose group develops highly-multiplexed products for human identification, such as the new GlobalFiler® STR kits that amplifies 24 loci simultaneously.
Here's a roundup of all of our posts and interviews from the recent American Association for Cancer Research conference held from April 18-22, 2015, in Philadelphia, PA USA. With over 19,000 researchers, physicians and interested stakeholders, this event had record attendance.
American Assoc. for Cancer Research Conference: what to look forward to.
April 29, 2015
PowerUp™ SYBR® Green Master Mix is a pre-formulated, optimized 2X Master mix for real-time PCR workflows. Some of the key features of PowerUp include exceptional specificity with dual hot-start mechanism, universal instrument compatibility, and compatibility with standard or fast cycling for results in less than 50 minutes.
In this video, Karen Li describes the dual hot-start mechanism and more.
April 27, 2015
Did you know that after the New York State attorney's office ordered four major retailers of herbal supplements to pull store-branded supplements from their shelves, they are now demanding ingredient and quality control information from the manufacturers of these products?
The tests for these products used DNA sequencing technology, but first some background is in order.
April 24, 2015
Bodour Salhia (Translational Genomics Research Institute, Phoenix AZ) shares her work in the search for potential biomarkers in cell-free DNA methylation signatures.
She presented a poster entitled "Whole genome bisulfite sequencing from plasma of individuals with metastatic breast cancer identifies putative biomarkers". Hear her describe her work in her own words.
April 24, 2015
Allen Mo (University of Connecticut) is studying small abnormal growths in the digestive tract called aberrant crypt foci (ACF), and is interested in particular genes up- or down-regulated in these structures and the surrounding stromal tissue.
He describes this work and why he uses not only the ArcturusTM Laser Capture Microdissection System, but also the Ion AmpliSeq™ RNA technology for efficient and economical targeted RNA-Seq.
AACR: Why the Oncomine Focus Assay?
Here Michaeline Bunting (Thermo Fisher Scientific) describes what is unique about the new OncomineTM Focus Assay product, and how attendees at the American Association for Cancer Research conference in Philadelphia have responded to it.
AACR Poster: The Oncomine Comprehensive Assay
April 24, 2015
Here in this video Dr. Jin Li of MolecularMD (Cambridge MA) shares his experience with both the OncomineTM Comprehensive Assay and his use and familiarity with the Ion AmpliSeq™ Technology for the past several years.
At the American Association for Cancer Research annual meeting in Philadelphia PA, Dr. Li presented a poster titled "Oncomine Cancer Panel: simultaneous detection of clinically relevant hotspot mutations, CNVs and gene fusions in solid tumors".
Behind QuantStudio 3 and 5 Instrument Development
April 24, 2015
In the world of optics and electronics, technology development means better quality for less money, and this is true when developing real-time PCR instrumentation such as the QuantStudioTM 3 and 5 Real-Time PCR Systems. We interviewed two Product Managers from Thermo Fisher Scientific.
Roland Tan and Levente Egry are both Senior Product Managers, and have been heavily involved in the development of the QuantStudioTM 3 and 5 Real-Time PCR instrument since its inception.
AACR | What's new in IVD platforms?
We caught up with Meri Bozzini (Thermo Fisher Scientific) about the latest about IVD instruments for genetic analysis from Thermo Fisher, and she describes in this video interview how NGS is of increasing importance in the clinical lab and how we are making progress with our IVD instrumentation to make it more accessible.
AACR Poster | Cell-free DNA targeted sequencing
Circulating cell-free DNA sequencing in cancer research is an area of great interest, which we've written about before. Dr. Dalia Dhingra (Thermo Fisher Scientific) shares the highlights of a poster she presented at the American Association for Cancer Research conference held in Philadelphia PA.
AACR | TaqMan™ Gene Fusion Assays as NGS data validation tool?
April 22, 2015
Similar to other Taqman™ Gene Espression Assays, the Taqman™ Gene Fusison Assay consists of an unlabeled PCR primer and a flourescent probe, but the Taqman™ Gene Fusion Assays are designed to span the fusion breakpoint in order to ensure specificity when detecting a particular fusion transcript. In this video Karen Li (Thermo Fisher Scientific) discusses the features and specifics of the new Taqman™ Gene Fusion Assays, as well as its capabilities as a tool to orthoganally validate data obtained through next-gen sequencing.
AACR Poster | Multiplexed control material for NGS
April 21, 2015
Kara Norman (Thermo Fisher Scientific) discusses the need for a complex multiplexed control material for next-generation laboratories and how important it is for quality control of sequencing data.
Her poster, entitled "A highly multiplexed control material to satisfy the complex needs of targeted next generation sequencing (NGS) oncology assays" has some interesting findings from different laboratories – as you might expect, some unexpected variability can come as a surprise.
AACR Poster | A cancer hotspot gene panel from 60 cells
Nicolo Manaresi is CSO of Silicon Biosystems SpA and presented a poster where their system could isolate specific disaggregated cells from an FFPE sample, then use the Ion AmpliSeq™ Cancer Hotspot Panel V2 to characterize samples with as few as 60 cells.
AACR | Is Cell-free DNA and CTCs an Either/Or Proposition?
April 21, 2015
In this interview André de Fusco and Dr. Paul Dempsey (CEO and CSO of Cynvenio Biosystems Inc.) share their perspective on the need to simultaneously isolate cfDNA and circulating tumor cells (CTCs) from the same sample.
AACR Poster | Verifying NGS with Sanger sequencing
April 21, 2015
When using an Ion AmpliSeq Panel, Dr. Edgar Schreiber (Thermo Fisher Scientific) shares how Sanger sequencing can easily be performed afterwards to verify the finding.
His poster, entitled 'Direct Sanger CE Sequencing of Individual AmpliSeq™ Cancer Panel Targets from Limited Amount of Input DNA using the Original Ion AmpliSeq Pre-Amplification Library', uses a very small fraction of the original pre-amplification library as the starting material for the Sanger sequencing reaction. Hear Edgar describe it in his own words.
AACR Poster | Using Ion Proton™ Sequencer to Study Osteosarcoma
April 21, 2015
Shintaro Iwata (Chiba Cancer Center Research Institute, Chiba Japan) presented a poster entitled "Targeted next-generation resequencing in pediatric osteosarcoma" using the Ion AmpliSeq™ Comprehensive Cancer Panel and the Ion Proton™ System.
In this interview he discusses his findings using this panel for this rare type of cancer research samples.
AACR | cfDNA & CTC by the LiquidBiopsy Platform
April 21, 2015
The American Association for Cancer Research exhibit hall debuted the new LiquidBiopsy™ Platform, and in this video Vidya Venkatesh (Thermo Fisher Scientific) shares the advantages of this system.
From collection and stabilization to excellent recovery and downstream analysis this system isolates both cell-free DNA (cfDNA) and circulating tumor cells (CTCs) from a single whole-blood sample.
AACR Poster | Rare mutations with QuantStudio 3D
April 21, 2015
Marion Laig (Thermo Fisher Scientific) describes her poster, "TaqMan Rare Mutation Assays for QuantStudio 3D Digital PCR System" in this video interview, combining 60 TaqMan SNP Genotyping research assays, with tested performance as low as 0.1% mutant allele frequency. She presented this poster at the American Association for Cancer Research meeting held in Philadelphia PA.
AACR Poster | Detecting CNV without normal controls
April 20, 2015
Jan Budczies (Charité University, Berlin Germany) presented a poster titled "Detection of copy number variations in beast cancer research samples using targeted sequencing without normal tissue controls" at the recent American Association for Cancer Research meeting in Philadelphia, PA.
In this video interview he highlights his approach to CNV sequencing data using the Ion AmpliSeq™ Cancer Hotspot V2 and the Ion PGM™ System.
A Sneak Peek at the Newest Real-Time PCR System | AACR
We caught up with Levente Egry, Sr. Product Manager (Thermo Fisher Scientific) at AACR to get the first look at the new QuantStudio 3 and 5 Real-Time PCR Systems. He gave us the inside scoop on new features, the role of Thermo Fisher Cloud and what excited him during the development process.
AACR Poster: Rare and novel FFPE alleles using LCM & NGS
A major theme at this year's American Association for Cancer Research meeting has been tumor heterogeneity, and Stephen Jackson (Thermo Fisher Scientific) presents a poster entitled "Identification of rare and novel alleles in FFPE tumor samples using laser capture microdissection (LCM) and Ion AmpliSeq™ sequencing technologies"
American Assoc. for Cancer Research - What To Look Forward To
April 14, 2015
Every year the American Association for Cancer Research scientific conference grows. Last year the attendance was over 16,000, and will be likely larger this year. Here's a select preview of the presentations and themes I'm looking forward to see.
Saturday, April 18 2015
Using STR genotyping for cheetah conservation
In this latest episode of #LabChat, Dr. Anne Schmidt-Küntzel discusses the efforts of the Cheetah Conservation Fund in Otjiwarongo, Namibia that uses microsatellite sequencing analysis to help preserve the genetic diversity of the existing cheetah population. About one-third of all surviving cheetahs live in Namibia.
Go Digital PCR Part 3: Copy Number Variation
Detecting and quantifying small percent copy number differences with a high degree of precision
In this edition we'll look at how dPCR is enabling researchers' abilities to achieve precise copy number variation (CNV) analysis.
A range of methods for CNV detection is currently available, including fluorescent in situ hybridization (FISH), comparative genomic hybridization (CGH), array comparative genomic hybridization (aCGH), real-time PCR (qPCR), next-generation sequencing (NGS), and dPCR.
ChIP-seq analysis of protein–DNA interactions on the Ion Proton™ System
Recent publication highlights usefulness of the Ion Proton System™ for ChIP-Seq applications with low input material
Epigenetic researcher and Scientific Director at National Jewish Hospital, Dr. Brian P. O'Connor recently teamed with the Thermo Fisher Informatics Applications Group to test Chromatin immunoprecipitation sequencing (ChIP-seq) analysis of protein–DNA interactions on the Ion Proton™ System.
Fragment Analysis by CE | Sanger Series Part 2
April 3, 2015
Introducing Fragment Analysis and how it can be used for a variety of applications formerly requiring manual gels and autoradiography.
Measuring DNA lengths
In Part 1, we took a look at using Sanger dideoxy chemistry to terminate elongating DNA strands, which are then separated via capillary electrophoresis (CE) in order to obtain single-base resolution and discrimination between single-base changes in DNA length.
Innovations & Perspectives: Human Identification Solutions Conf., Madrid
April 1, 2015
Recently a two-day Human Identification Solutions Conference was held in Madrid Spain, sponsored by Thermo Fisher Scientific. Here are a few themes, quotations and observations made from presentations covering a wide range of topics.
Public awareness affect forensic genetics
One theme that arose more than once was the importance of public engagement and how perception of forensics affect the entire field. Prof. Dr. Peter Schnieder (Institute of Legal Medicine, Cologne Germany) called it the 'CSI Effect: where everything is perfect, just like on TV'.
Increasing the sensitivity of low-level mutations using COLD-PCR
March 30, 2015
The lower limit of sensitivity using next-generation sequencing is generally accepted to be 5%. The reason for this detection limit has to do with the error rate of the platform, rather than as a function of base coverage read depth.
A graphical illustration of this sensitivity limit using the SOLiD®
Sequencing System is still available online, as an interactive flash-based graphic. The Variant Frequency in Hetergeneous Sample is a horizontal slider, and top 'Coverage Required for Detection of Variants at 05% Frequency in a Hetergenous Sample' reads 99% accuracy, 450x coverage.
Go Digital PCR Pt. 2 – QuantStudio 3D
March 27, 2015
How to deliver absolutely attainable digital PCR, simply and affordably
This is the second in a six part series to introduce you to digital PCR (dPCR). In this edition, we'll take a look at how QuantStudio® 3D dPCR from Life Technologies offers simplicity, affordability, and most importantly, absolute quantification attainability.
QuantStudio® 3D Digital PCR System helps expand the use of dPCR
AGBT 2015 Conference Post Roundup
The annual Advances in Genome Biology and Technology (AGBT) conference recently concluded in Marco Island, Florida USA. Here's a summary of all of our posts and interviews.
Blog Posts
Here's what we're looking forward to at AGBT highlights several presentations and activities at this conference.
Shotgun Metagenomics | AGBT 2015
March 25, 2015
At the Advances in Genome Biology and Technology, there were a wide variety of applications from the scientific posters that illustrate the flexibility of sequencing to solve a variety of problems. MRI Global is a not-for-profit contract research organization, and Dr. Jonathan Jacobs, (or as he is known on Twitter as @bioinformer) research is centered on air sample for biosurveillance and how pathogens may impact public health using the Ion Proton™ Sequencing System.
Using QuantStudio® 3D for CYP2D6 polymorphism genotyping
March 23, 2015
QuantStudioTM 12K Flex pharmacogenomics extended to allele-specific copy number with digital PCR
The rapidly-growing field of pharmacogenomics (PGx) is the study of genetic variation as it relates to drug response, determined by variation in drug metabolism enzyme and transporter (DMET) genes. The significance of PGx will increase as personalized medicine becomes more widely adopted.
A flexible workflow from sample preparation through
Sanger Sequencing by CE 1: Foundations
In 1951, Fred Sanger first determined the sequence of two proteins (the bovine insulin A and B forms), showing that they were distinct molecules, and for this he was awarded a Nobel Prize in Chemistry in 1958. In 1965, he lost the race to be the first to sequence a tRNA molecule. In 1977, he introduced the "di-deoxy" chain-termination method, which earned him a second Nobel Prize in Chemistry in 1980 (which he shared with Paul Berg and Walter Gilbert, of the Maxam-Gilbert chemical-based method of sequencing ).
Ion Torrent Technologies Update - AGBT 2015
March 18, 2015
Alan Williams of Thermo Fisher Scientific shares highlights from his Advances in Genome Biology and Technology workshop, reviewing data from the Ion Hi-Q™ chemistry with its improved accuracy on both the Ion PGM™ and Ion Proton™ platforms with Ion Chef™ automation.
He also reviews long-read (400 base-pair) results on the Ion PI™ Chip, as well as new data from the upcoming Ion PII™ Chip, to be released later this year.
Analyzing 700+ Germ Cell Seminoma microRNAs | #LabChat
Using a panel of 740 microRNAs to research testicular germ-cell seminomas, Dr. Martin Rijlaarsdam of Erasmus University Medical Center in the Netherlands describes in this LabChat their characterization of unique biomarkers.
Go Digital PCR: Pt 1 – Next-Gen Quantification
March 13, 2015
Go Digital PCR: Pt 1 – Next-Gen Quantification begins our six-part series introducing you to digital PCR (dPCR), what makes it next-gen technology, and how it compares to real-time quantitative PCR (qPCR) and traditional PCR.
What Is Digital PCR?
To date, qPCR has been a powerful and sensitive gene analysis techniques used for a broad range of applications. As the name suggests, qPCR measures PCR amplification as it occurs, unlike traditional PCR, which collects results after the reaction is complete, making it impossible to determine the starting concentration of nucleic acid.
AGBT: Single-cell neurogenetics
March 11, 2015
We profiled 'Taking the Sequencer to where the samples are – the open ocean' about the University of Florida – Saint Augustine's work, highlighting Dr. Leonid Moroz and his RNA-Seq work on ctenophores (an organism that shows remarkable neuroregenerative properties).
At the Advances in Genome Biology and Technology conference we interviewed a close collaborator of his, Dr. Andrea Kohn also at the University of Florida, who gave a presentation entitled "Single-Cell Methylome Profiling: Integration of a Transcriptome and Methylome From Individual Identified Neurons".
AGBT: 25,000-plex PCR - Finding the Genetic Heroes
Bobby Sebra of the Icahn School of Medicine at the Mount Sinai University Hospital discusses the development of a 25,000-plex Ion AmpliSeq™ Panel as part of the Resilience Project, at the recent Advances in Genome Biology and Technology conference.
We've talked about the Resilience Project before on Behind the Bench, and Dr. Sebra talks about the technical underpinnings and performance of their large AmpliSeq™ Panel.
March 5, 2015
David Guttery of the University of Leicester UK spoke with us at the Advances in Genome Biology and Technology meeting, and shared his experience in developing an Ion AmpliSeq™ Panel for targeted sequencing of tumor-related genes for cancer research.
AGBT: What is a Liquid Biopsy?
At the Advances for Genome Biology and Technology, Dr. Jason Johnson of Thermo Fisher Scientific presents a new capability to examine circulating free DNA (cfDNA), circulating tumor cells (CTC) and germline DNA variants from the same sample.
And from there he discusses Ion AmpliSeq™ panel analysis with sensitive minor allele frequency detection.
AGBT: Sequencing Over 100 Exomes per Day
Claritas Genomics CEO Dr. Patrice Milos shares the highlights of her presentation along with feedback about the new Ion Hi-Q™ chemistry in this interview from the Advances in Genome Biology and Technology. Their laboratory automation and flexible sequencing service as an Ion Torrent™ Certified Service Provider offer fast turnaround time with the ability to scale from smaller to larger whole-exome sequencing projects.
AGBT: CTC's and Allele Frequency - How Low Can You Go?
March 3, 2015
Currently there is a lot of interest in circulating tumor cells (CTC's) as a rich source of biomarkers for the early cancer detection. For these enrichment methods, the detection of cancerous cells is clouded by the presence of many normal cells. Thus there is a need for sensitive somatic mutation detection.
AGBT: 12 hour Aneuploid Detection
March 3, 2015
Adam Harris of Thermo Fisher Scientific R&D shared highlights of his poster called "Rapid Detection of Aneuploidy from Multiplexed Single Cell Samples" he presented at the Advances in Genome Biology and Technology Conference.
Research using complete microbial de novo genome assembly makes advances, thanks to NGS
March 2, 2015
Mate-pair libraries for microbial whole genome sequencing now available
Earlier this year, a new microbe dubbed a "methanogen" was reported in the now-thawing permafrost of northern Sweden as part of a study. This microbe appears to play a significant role in methane release due to global warming. Around the same time, a Russian team's study revealed new insights into the genetic changes undergone by Mycobacterium tuberculosis, which may help researchers understand how the tuberculosis-causing microorganism can achieve drug resistance.
AGBT: Customizing Your Ion Reporter™ Software
Our online analysis tool, Ion Reporter™ Software, now offers more flexibility for the power-user while maintaining an easy-to-use interface. Dr. Simon Cowley from Thermo Fisher Scientific presents the highlights of a talk where he discusses how to Create your informatics - customize variant calling parameters, Ion Reporter™ workflows, and command-line, open source informatics pipeline"
How low a variant frequency can you detect?
Dr. Simon Cawley, Thermo Fisher Scientific, describes at Advances in Genome Biology and Technology improvements to Applied Biosystems Sanger Capillary sequencing, Applied Biosystems digital PCR, and the combination of Ion AmpliSeq™ Panels with Ion Reporter™ software.
AGBT: Ion AmpliSeq Transcriptome Poster
At the Advances in Genome Biology and Technology conference Dr. Kelli Bramlett presented a poster entitled "Global gene expression profiles from breast tumor samples using the Ion AmpliSeq™ Transcriptome Human Gene Expression Kit".
In this video interview she discusses differential gene correlations to two different orthogonal gene expression measurements: a panel of over seven hundred TaqMan® genes, and whole-transcriptome RNA-Seq.
During the Advances in Genome Biology and Technology Conference Dr. Mark Andersen of Thermo Fisher R&D shared highlights of his presentation entitled "You want to put what where? Ion AmpliSeq™ technology, automation, any sample". If you want to see what else the Ion Chef™ instrument can do, take a look!
AGBT: The Avalanche of NGS Data
At the Advances in Genome Biology and Technology Conference Dr. Adam Harris of Thermo Fisher Scientific R&D shared highlights of a presentation called "An avalanche of data: long reads and a speedy workflow with isothermal amplification". How much time does this process save in template preparation? Watch and find out!
AGBT: Ion AmpliSeq™ Designer for easy multiplexing
Francisco Hernandez-Guzman, Product Manager, shares the latest improvements to the online Ion AmpliSeq™ Designer tool for efficient targeted next-generation sequencing.
AGBT: Update on Ion Reporter™ v.4.4 with Carl Dowds
Here at Advances in Genome Biology and Technology there was a software bioinformatics demonstration session; we spoke with Dr. Carl Dowds Sr. Product Manager of Informatics Applications about the latest version of Ion Reporter™ Software and what it offers.
How can you analyze a single cell?
February 24, 2015
A novel technology to automate single-cell and cell-free DNA analysis
There is continued, growing interest in the analysis of single cells. For example, in 2009, Thermo Fisher Scientific's scientists collaborated with a group at the Wellcome Trust Cancer Research Institute (Cambridge, UK) for whole-transcriptome RNA-Seq from a single cell (for more information about this work an Application Note is available.)
The Unexplored Diploid Landscape
February 20, 2015
Revealing gene and protein diplotypes through multiple haplotype-resolved human genomes
Studying genetic variation generally lays aside consideration for variants as they line up along a particular parental allele. While we accept that the human genome is diploid, in practice having two different forms of each allele is taken as individual homozygous or heterozygous single nucleotide variants at a given base position, rather than a string of variants lined up along distinct haploid genes.
Targeted Sequencing or Whole Exome Sequencing? The Debate Continues
February 18, 2015
Five years ago, Dr. Richard Gibbs of the Baylor College of Medicine gave a memorable plenary presentation at the annual Advances in Genome Biology and Technology conference in Marco Island, Florida."
Entitled "Genome Sequencing to Health and Biological Insight", in his introduction he illustrated the seesaw battle between whole-genome or whole-exome sequencing approaches to a targeted sequencing approach by showing a drawing of two aluminum cans of popular cola soft-drinks, as a cartoon with boxing gloves. Illustrating this question of approaches as a battle, he said that while he favored the whole-genome / whole-exome sequencing approach he didn't see any end to this struggle for the foreseeable future.
9 Sequencing Moments That Mattered In 2014
February 17, 2015
From clinical research, agricultural biotechnology, metagenomics, and technical improvements, here's a brief video roundup from yourteam at Behind the Bench of the major next-generation sequencing "moments that mattered" in 2014.
Here's What We're Looking Forward To At AGBT
February 17, 2015
AGBT is a "recognized cornerstone for the genomics research community" and is the meeting where new technologies in development report their progress. I will be there reporting the latest news and here's what's on my agenda while at Marco Island.
The Winners of the Ion Torrent Transcriptome Profiling Grant Program
February 17, 2015
What would you do with free transcriptome sequencing? We asked that question in November 2014 during the Ion Torrent™ Transcriptome Profiling Grant Program. And over 200 researchers world-wide answered by submitting grant proposals for up to 24 samples of free sequencing using the Ion AmpliSeq™ Transcriptome Human Gene Expression Kit, with sequencing services provided by our Certified Service Providers. Here are the winners...
While testicular cancer is relatively rare (about 8,500 cases in the US annually) it accounts for the most common solid tumor type in men aged 15 to 35. The vast majority (95%) are germ cell tumors, of which about half are called seminomas. These are usually slow growing and when discovered early are often treatable. The other half are called nonseminomas, are often more aggressive and can metastasize to other locations in the body.
A few months ago 16S metagenomics was highlighted on this blog, where researcher's 16S custom amplicons can be easily analyzed with a new capability within Ion Reporter. Useful resources, such as the Metagenomics Applications Group in the Ion Community and the Ion 16S Software Steps for Custom Primers are discussed in that prior post.
February 6, 2015
A few weeks ago we highlighted human identification using next-generation sequencing. Dr. Kayser is asking an interesting forensic research question: what about determining the identity of unknown perpetrators, by obtaining externally identifyable phenotypes?
An example of his prior work in 2011 described the use of six SNPs for identifying blue or ********* color. (This paper used an assay called SNaPshot® Multiplex System and capillary electrophoresis.)
February 4, 2015
Silicon Biosystems is a new company based in Italy that is developing a novel platform for solving the problem of heterogeneity of cells from tissues of many types. (For example, it is well-established that cancer is comprised of at least six cell types.) Their system uses microfabricated electrodes on a silicon chip married to a fluidic system, where a set of 9 electrodes form a di-electrophoretic 'cage' that enables single-cell trapping (as well as manipulation). These chips are called a DEPArray.
February 2, 2015
In case you missed any of our coverage of the recent Plant and Animal Genome conference (San Diego, California USA) here's a roundup of all of our posts and interviews.
2015 Plant and Animal Genome Conference XXIII Conference in San Diego, California USA.
Blog posts:
Developing virus-resistant and herbicide-resistant cassava for Africa.
Sharing highlights of two presentations from a session on Cassava, an important food crop in Africa.
January 30, 2015
Nitrogen availability is one of the major limiting factors to crop growth. In the developed world where the use of nitrogen fertilizers are ubiquitous, their over-use causes nitrification of the environment and damaging algal blooms, in addition to atmospheric pollution. In the developing world, little access to fertilizers by smallholder farmers limits crop production, much more than the availability of water.
January 28, 2015
At the Plant and Animal Genome conference (held annually in San Diego California), many specialized sessions are devoted to specific crops and livestock. For example, on one afternoon there were 11 concurrent sessions, among which were Coffee Genomics, Curcurbit (otherwise known as pumpkins, squashes and gourds), and Domestication Genomics.
January 27, 2015
#LabChat interview with Dr. Mitch Garcia of Cytolumina
Circulating Tumor Cells (CTC) have rightly attracted a lot of interest in the field of cancer research, and in this interview Dr. Mitch Garcia discusses work that comes out of Dr. Hsian-Rong Tseng's group at the University of California, Los Angeles.
January 26, 2015
5% of Colorectal Cancer has an inherited source
Colorectal cancer is the third most-common cancer worldwide, with over 130,000
new cases in the United States in 2014 alone. About 5% of these cases are inherited in an autosomal dominant fashion, and the two major types of these inherited cases are called familial adenomatous polyposis and hereditary non-polyposis colorectal cancer (HNPCC).
A king's remains discovered in a car park
King Richard III had a short but notable reign (1483-1485), starting with the death of his brother, his 12-year old nephew Edward V declared an illegitimate heir to the throne, then Richard III ascending to the throne. His tumultuous reign ended when he was killed in battle by the forces of Henry VII. At the age of 32, he was the last of English Royalty to die in battle, and the only king whose remains were not known.
January 22, 2015
Kristian Ridley from Thermo Fisher Scientific shares what is new about genetic analysis from the exhibit floor of the recent Plant and Animal Genome Conference, along with observations about which products Thermo Fisher customers are interested in.
January 22, 2015
Speaking at the Plant and Animal Genome Conference, Dr. Jason Boone discusses Floragenics' work on a variety of animal genomes in varied locales worldwide, as a genomics service provider. They have used genetics to track the pygmy elephant for conservation efforts, and also discusses the use of Ion AmpliSeq™ to perform rapid genotyping-by-sequencing with barley, and the cross-platform accuracy they've experimentally determined.
January 21, 2015
Don't be paralyzed by too many choices for custom gene expression and genotyping assays
Having freedom to choose is a great thing. Having too many choices to choose from can be paralyzing. We have all been at a restaurant where the extensive menu meant that not only did it take a long time to make up your mind on what to have for dinner, but also it generated some concern that the optimal choice was not made. And then once the meal arrives for everyone at the table, this concern can likely turn into regret.
GeneSeek a genetic service provider, has been characterizing traits for agricultural biotechnology companies since 1998, with many capabilities across the genetic analysis spectrum including genotyping and SNP microarray technologies.
January 20, 2015
Cassava is a vitally important food crop worldwide. Otherwise known as manioc; in North America a well-known derivative form is tapioca. Native to northern Brazil, it spread throughout the South American continent and was carried by the Spanish and Portuguese explorers to the West Indies, then to the continents of Africa and Asia. Cultivated worldwide, an estimated 800 million people depend on it (500 million in Africa) as a main source of calories in their diet (up to 60%).
January 20, 2015
Dr. Li Song from the University of Missouri uses the QuantStudio® 3D Digital PCR System to speed up their characterization of transgenic soybeans.
January 19, 2015
Formalin Fixed, Paraffin-Embedded (FFPE) samples are challenging to work with. Dr. Nicole Pfarr discusses her experience with Ion AmpliSeq™ Panels (they currently use eight routinely, both standard and custom ones) on the Ion PGM™ System.
At the recent Plant and Animal Genome Conference, Monsanto computational biologist Dr. Lex Flagel discusses their work on the Western Corn Rootworm and the use of genotyping-by-sequencing to understand the genetic mechanism to Bt toxin resistance.
The International Rice Research Institute, located in Los Baños, Philippines, aims to reduce poverty and hunger, improve the health of rice farmers and consumers, and ensure environmental sustainability of rice farming.
January 16, 2015
James Schnable from the University of Nebraska-Lincoln has developed a technology called 'tunable Genotyping by Sequencing' (tGBS) that provides much better read-depth compared to conventional genotyping-by-sequencing approaches.
Read more...
January 13, 2015
Is the EagleTaq Universal Master Mix equivalent?
We are living in a remarkable, connected age – where all the world's information is just a click away. It is hard to imagine that as few as twenty years ago to look up a publication abstract it meant going to an academic library and pull out the ISI Abstracts
reference volume(s). It seems quaint now, as looking up abstracts of journal articles are just a few clicks away.
You may be familiar with the Ion AmpliSeq™ Panels for highly multiplexed, single-tube PCR for next-generation sequencing target selection for human or mouse genes. You also may be familiar with the online AmpliSeq.com tool for making custom designs, as well as accessing gene lists and target coordinates for the standard research panels that are offered.
But did you realize that there are now several additional reference genomes to design a custom research panel from?
January 9, 2015
Dan Tonge has been working with the Ion Torrent™ platform for some time, and currently is working with uncultured fungi at very low concentrations. He discusses his approach toward how these amplicons are designed, and how they tested and challenged their ability to distinguish between closely related fungal species.
January 7, 2015
A huge neuroscience project is just getting started: will you be a part of it?
When you think of the largest government undertakings that had the largest impact in the modern era, what comes to mind? (By 'impact' you can consider the economic, social, or scientific ramifications excluding war, which of course is a separate question.)
First (and most recent) is the Apollo Moon Project. According to historians it was driven more by global politics rather than scientific discovery, cost some $24B USD in 1973 dollars (equivalent to $170B USD in 2005) but as far as a large, ambitious government projects that made great progress as a society it is difficult to argue against.
January 6, 2015
A few talks and a workshop of interest at PAG XXIII January 10-14, 2015
The Plant and Animal Genome Conference calls itself 'the largest Ag-Genomics meeting in the world', and there is no reason to contest that. Do you work with arthropods like psyllids? There's something for you. Aquaculture? There's a day-long program devoted to it. Cacao? A whole afternoon set aside, now of particular importance given recent publicity around the growing worldwide chocolate deficit.
January 2, 2015
We've all grown accustomed to turning to YouTube to answer questions ranging from child care to yoga tips, or even how to deep fry a turkey. Back in 2012, we launched the Ask TaqMan video series with the belief that researchers are hungry for knowledge on a topic that's very near and dear to our hearts: qPCR.
As it turns out, A LOT of people are in need of qPCR help, and in 2014 alone, the Ask TaqMan videos were viewed over 120,000 times! So what were those burning qPCR questions in 2014? Let's take a look at the top 5 for the year…
December 29, 2014
Joel Dudley of the Icahn School of Medicine, The Mount Sinai Hospital (New York New York USA) discusses big data in the age of genomics. As a data scientist involved with the Resilience Project and its data-driven approach toward new discoveries with major research implications.
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text:
An Open-Label, Multi-Center Phase II Study of the BRAF Inhibitor RO5185426 in Patients with Metastatic or Unresectable Papillary Thyroid Cancer (PTC) Positive for the BRAF V600 Mutation and Resistant to Radioactive Iodine (NO25530B)
Metastatic Thyroid Cancer | Unresectable Papillary Thyroid Cancer
What is the purpose of this trial?This research study designed to look at whether the drug vemurafenib (also called Zelboraf or RO5185426) can help stop the growth and spread of cancer cells in patients with papillary thyroid cancer (PTC) which has spread to other parts of the body (is metastatic), cannot be removed by surgery (is unresectable), and was or has become resistant to radioactive iodine treatments. The study will also collect additional information about the safety of Vemurafenib Vemurafenib is approved by the U.S. Food and Drug Administration (FDA) for the treatment of late stage melanoma whose tumors show a gene mutation called BRAF V600. It is not approved for treatment of PTC so in this study the use of Vemurafenib is investigational, meaning that it is not approved for routine treatment for PTC and can only be used in PTC in research studies such as this one. Secondary purposes of the study include • whether vemurafenib may be of benefit to patients previously treated with sorafenib who have PTC • pharmacokinetics of vemurafenib (to see how long the study drug stays in your body and how well your body can get rid of the drug) • if treatment with vemurafenib will have an effect on proteins in your blood, tumor or skin that will provide information on how your tumor responds to treatment. These proteins are called 'biomarkers.' You must allow testing of your samples for biomarkers for at least 5 years in order to participate in this study. Exploring biomarkers are an important part of this study because we are trying to identify what the right treatment is for different types of patients. • evaluate the Roche Companion Diagnostics (coDx) cobas® 4800 BRAF V6000 Mutation Test (to detect the BRAF gene mutation) from tumor samples. The 4800 BRAF is FDA approved for detecting the BRAF mutation in metastatic melanoma but is investigational in this study
Participation Guidelines
- Age:
- 18 Years and older
- Gender:
- Both
Click here for detailed information about who can participate in this trial.
- Sponsor:
- Hoffmann-La Roche
- Dates:
- December 2011
- Last Updated:
- Study HIC#:
- 1111009271

subset: commoncrawl


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What time should we get out there on sunday to pay!
What time should we get out there on sunday to pay!
I plan to be there around 8 - 830 and take registration.
After a rainy night the weather looks good this morning, see you at Iroquois!
Where we have strong emotions, we're liable to fool ourselves. Carl Sagan
Who won?
Acts 4:12
There is salvation in no one else, for there is no other name under heaven given to people by which we must be saved.
There are currently 1 users browsing this thread. (0 members and 1 guests)


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IP Registration and Revalidation for Linux/Unix
1. Go to https://ipreg.uthsc.edu and read the Terms of Service and click the 'I Agree' button.
2. Log in with your NetID and NetID password. What is my NetID?
3. Fill out the information on the form. You must fill out all the information to continue. When finished filling out the form, click on the 'Update' button to continue.
4. Click on the Exit button to finish the process. Should you run into any problems accessing the network after clicking on the Exit button, restart the computer. Should your problem persist, call the Helpdesk at 448-2222


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Four first half tries got the Highlanders their second win of the season.
The Highlanders played their best half of rugby to beat the Blues in Dunedin. After leading 29 – 7 at half time, the Blues made a slight comeback but still lost 38 – 28.
Ben Smith was the star for the Highlanders, his name being mentioned in almost every phase, scoring two tries and being involved in the other. He was an ever present in a Highlanders backline that ran everything and forced the Blues to make more than 100 tackles in the first half alone. Smith opened the scoring before Tamati Ellison doubled the lead. Smith scored his third and the bonus point try was wrapped up by Hosea Gear. The attacking prowess shown was what everybody expected at the start of the season.
The Blues scored their first try on the half time hooter, to at least show something for being on the field for the first forty. Rene Ranger and Steven Luatua gave the Blues hope early in the second half, reducing the deficit. Colin Slade had other ideas though, kicking a few more penalties, along with a kick from Hayden Parker, to keep the Blues at bay.
A late defensive effort from the Highlanders ensure that the Blues were only playing for a bonus point and not a victory. That bonus point for the fourth try was achieved a few minutes after the hooter, Luke Braid awarded for his efforts. The bonus point kept the Blues' hopes of reaching the play-offs alive, but with a tour to South Africa still to happen, this might be the end of the road.
The Highlanders are sure to take the confidence from this match to win a few more and prevent themselves from finishing as wooden spoonists on the overall log.
Highlanders: 38 (29)
Tries: Ben Smith (2), Tamati Ellison, Hosea Gear
Conversions: Colin Slade (3)
Penalties: Colin Slade (3), Hayden Parker (1)
Blues: 28 (7)
Tries: Waisake Naholo, Rene Ranger, Steven Luatua, Luke Braid
Conversions: Baden Kerr (4)
By Richard Ferguson


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All is Dust and DNA
Jim Stump explores the meaning of the Lenten season from the perspective of evolutionary creation.
The Resource Finder has powerful filtering features — check out the tutorial to learn more.
Jim Stump explores the meaning of the Lenten season from the perspective of evolutionary creation.
From 2012: Many secular and many evangelical voices agree on one 'truism'—that if you are an orthodox Christian with a high view of the authority of the Bible, you cannot believe in evolution in any form at all. New Atheist authors such as Richard Dawkins and creationist writers such as Ken Ham seem to have arrived at consensus on this.
New BioLogos content editor Brad Kramer reviews Chapter 1 of "Surprised by Scripture" by NT Wright, and sees Wright's work as a signpost for the future of the science/faith debate.
For Augustine, the literal sense of scripture is not a scientific narrative of physical reality, but a liturgical and poetic narrative of God's awesome power at the dawn of history. This is a vital thread in the story of how to read Genesis.
Everyone is so worried about success and getting this or that honorable diploma—the people here are smart and understand many complex things perfectly—but it's a long distance from the head to the heart.
Christ is the reason the natural sciences work: he is the source of the laws of nature, and he contains and underpins the natural sciences. Christ is the pattern for all created things, while the Spirit – his presence in the created world – breathes the divine fire into the equations.
We now take for granted an understanding of the Christian story that was largely worked out by Paul and later theologians. Even though the Gospels were composed after Paul's letters, they were concerned to tell the story itself in all its strangeness as it had been preserved by the first generation of Christians. And what we find in the stories themselves is the shock and wonder and surprise that the resurrection caused.
Four Views on the Historical Adam shows that evangelical Christians are far from agreeing on the particulars of Adam and Eve. But this book also makes clear that there is strong agreement among evangelical Christians on the fundamentals of the Christian faith. We agree that all Scripture is inspired by God and authoritative, even while disagreeing on the interpretation of some passages.
The key point of continuity between redemption and creation is the idea that the future can be different from the past, i.e., the key is eschatology. More abstractly put, God does new things.
All of you who do science, all of you who teach or research or you're involved in engineering or medicine or education, or biology or chemistry or physics—you are doing a noble thing. You are thinking God's thoughts after him. … You are obeying God's command given way back in Genesis to exercise dominion, to learn about, to be curious and discover and steward the earth.
The Psalmist is saying that when we walk outside and look up, the heavens are telling us two things about God: they tell us about his glory, and they tell us about what his hands can do.
Some people who brook no "god of the gaps" arguments anywhere else look to these three moments as more reasonable places to insert God into natural processes: God spoke matter/energy into existence, God made life out of lifeless matter, and God breathed a soul into human beings.
We need to hear stories from others who have wrestled with evolution and Christian faith. What arguments made them change their views on science? How did they hold fast to their relationship with God? The essays in this series will eventually comprise a book, provisionally titled, "Evolving: Evangelicals Reflect on Evolution."
The idea that the story we know is only the very beginning raises a new question in place of Feynman's objection that Christianity is provincial. Is it presumptuous to claim that in such a grand universe, possibly with intelligent life arising in many places, the redemption and transformation of the entire cosmos starts here, on our pale blue dot?
Over the next few months, with permission from Yale University Press, BioLogos will offer edited versions of chapters from John Polkinghorne's best books, Belief in God in an Age of Science and Theology in the Context of Science, in order to help readers delve more deeply into some of his most important ideas.
So what then does Resurrection mean? For Benedict it represents a new dimension of reality breaking through into human experience. It is not a violation of the old; it is the manifestation of something new.
There's a word beneath the water, and the Bow River belongs to God. Have you been listening?
The God who created the cosmos is the God who came to us as a child in Bethlehem.


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Saturday, April 28, 2012
Reader Ideas Week of 4/29/2012
27 comments:
- Marcita said...
What a nice shetch. This is my card.
- Sun Apr 29, 09:28:00 AM CDT
- Yvonne B. said...
I really like this sketch--you can do a lot with it and I took it in a little bit of a different direction for my card! Thank you!
- Sun Apr 29, 12:08:00 PM CDT
- Message Keeper said...
So many ideas for this sketch! Hope I can do more cards using this sketch when my time permits!
- Mon Apr 30, 03:23:00 AM CDT
- Heidi H said...
This sketch has so many possibilities! Loved it!
- Mon Apr 30, 05:34:00 PM CDT
- Teresa Zuehls said...
Love this sketch! It was easy to use.
- Mon Apr 30, 08:18:00 PM CDT
- Adriana Benitez said...
Great sketch! Had to make my panels a little wonky after dropping an inked stamp, but hey - it worked like this, too! lol
- Tue May 01, 08:40:00 AM CDT
- Borqna said...
Love this sketch!
Thank you!
- Tue May 01, 12:55:00 PM CDT
- Emily said...
This my first time participating in a cps sketch challenge. I hope to join in on many more!
- Tue May 01, 05:10:00 PM CDT
- Vickie O'Dell said...
I get so much inspiration from the sketches and layouts. Thanks for the creativity booster!
- Wed May 02, 12:46:00 AM CDT
- CraftyRia said...
I love sketches and I love Skipping Stones Designs! I especially love when they both go together. I hope you enjoy the card I made for this challenge.
- Wed May 02, 08:53:00 AM CDT
- Caroline said...
I really like this sketch and I had tons of ideas to play with it. I finally chose to rotate it...
- Wed May 02, 08:47:00 PM CDT
- Michele said...
Gosh... your sketch was the answer to my mind block!! Thank you!! I went a little crazy with it. :-)
- Thu May 03, 01:43:00 AM CDT
- Rachael said...
Lovely. I love the paneling in this sketch.
- Thu May 03, 02:10:00 PM CDT
- Angie said...
I enjoyed playing with this sketch!!!
- Thu May 03, 02:41:00 PM CDT
- Marianne said...
This is just my second time playing along at CPS-Card Sketches. This sketch really inspired me :) Lots of fun!
- Thu May 03, 02:43:00 PM CDT
- Charlotte said...
Hi! I have just found you here in Blogland. And I am so glad I did. I so enjoyed making my card for the challenge. And I especially enjoyed viewing all the wonderful cards others have made.
- Thu May 03, 05:57:00 PM CDT
- Paperesse said...
A very nice sketch and a nice way to use up left over papers.
- Thu May 03, 07:11:00 PM CDT
- Marion said...
Thanks for the great sketch!
- Thu May 03, 10:05:00 PM CDT
- Colien said...
Thanks for a nice challenge. Love this sketch and hope to use it more after this.
- Fri May 04, 02:47:00 AM CDT
- Mae said...
YAY, I am finally able to link up and be apart of the challenge again. I've missed out these past few weeks & glad to be back!
- Fri May 04, 03:28:00 PM CDT
- Mae said...
YAY, I am finally able to link up and be apart of the challenge again. I've missed out these past few weeks & glad to be back!
- Fri May 04, 03:28:00 PM CDT
- Kim said...
Just found your blog! Thanks for the challenge :)
- Fri May 04, 04:24:00 PM CDT
- Dorothy S said...
Thank you for another fun sketch, and the variety of inspiration by DT members.
- Fri May 04, 10:39:00 PM CDT
- Cynthia said...
I was stumped at first, but I really like what I ended up creating! Thanks for the inspiration!
- Fri May 04, 10:49:00 PM CDT
- Laura said...
i had a lot of fun with this sketch! Thank YOu!
- Fri May 04, 11:03:00 PM CDT
- Kimberly said...
Love the sketch! Just linked up my card. Thanks for the great inspiration!
- Sat May 05, 06:07:00 PM CDT
- ☼Carolina Artesanías☼ said...
pretty cards by CPS-263 sketch
This is my card:
from Chile
Carolina ArteScrap
- Fri May 25, 08:05:00 AM CDT


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Hours & Admissions
Get information on our hours, contact phone numbers and admission prices for all ages, plus teachers, military, Art After 5, and 3-Day passes.Hours & Admissions
Getting Here
Follow these directions to come by car, bus, bike, or plane. Everything you need to know to make getting here and parking simple.You're on Your Way!
Tour Options
Add to your enjoyment and enrich your experience. Find out about the various docent led and self-guided tours at the Museum of Art, Circus Museum and Ca' d'Zan.Select Your Tour Now
Group Visits
Learn about the special docent led and self-guided group tours available for K-12, college students, and special interest groups. Make the Ringling a shared experience.Group Visits
Plan Your Visit
Plan ahead to make the most of your visit. Get all the information you need about everything from strollers and shopping to accessibility and suggested itineraries.Start Planning Your Visit
Look over our menu of delicious dining options: Treviso's mix of classic cuisines, the Banyan Café's family-friendly fare, and the Museum Cafés coffee house vibe.Dining at The Ringling


text:
From the bottom of our hearts, we want to thank you. A few weeks ago, we told you about the hay emergency facing American horse caregivers this year. We told you that persistent drought has put hay in dangerously short supply, and that if we don't pull together and do something, horses will die.
We asked you to help us feed horses by contributing to our Hay Bale-Out initiative, and we were overwhelmed by the generosity of those who answered our call.
Here's what happened next.
We told equine rescue groups the good news: The ASPCA has your back, and we can provide hay to help get your horses through the winter. We asked them to tell us what they needed.
Since then, equine groups have been flooding us with requests.
We're deep in the process of working closely with these groups to meet their hay needs, and our equine rescue friends tell us this assistance couldn't come at a better time.
"This year is setting up to become a 'perfect storm' for equine neglect, as we are already seeing a huge increase in calls and skinny horses," Gail Vacca of the Illinois Equine Humane Center told us. "Thank goodness many will find relief due to the efforts and support of the ASPCA."
We're so grateful for your help feeding horses this year. If you haven't yet given to this critical effort, it's not too late! Every little bit counts, and it all goes directly to feeding horses.
Watch ASPCA.org for updates on this life-saving project, and if you haven't already, consider being a part of it.
2 situations I have come across in the past year. One is a 4 year old Australian Shepard that is obese left in the backyard with food and water often for a week by himself, while the owners go on vacations. He has access to an indoor room and is not chained. Is this abuse or neglect?
Another is an acquantanse telling me that she got a puppy and somehow the puppy got thrown out of the window and ended up breaking his back. She said she took the puppy to the Vetrinarian and he refused to euthenize the dog because surgery would've taken care of the situation. She decided that she did not wish to spend the money and took the puppy and dropped it off at the animal shelter. She asked me not to mention the puppy to her 7 year old boy. And she recently mentioned that she had to emotional problems last year, which dated back to when this situation took place. Please advise me on what we need to do if anything can be done. Thank you


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Griquas got the win but Southern Kings Director of Rugby Alan Solomons got questions answered in his team's final Super Rugby pre-season outing.
Griquas scored late to win 30-27 and Solomons told Keo.co.za on the balance of performance the right team got the winning result.
'Pre-season matches are about more than the result but every player and team wants a winning one,' he said. 'We did not get enough ball to enforce our way of play and all credit to the effort of Griquas. They dominated the lineout and denied us a platform. It is hard to play any game without ball from set piece and if the boys didn't know that they certainly will now.'
The Kings historic Super Rugby debut is Solomons' focus and he said bigger than the result in Port Elizabeth was that captain Luke Watson and lock Steven Sykes got through the match in what was their first appearance after lengthy injury lay-offs.
'We gained from those objectives in that the players returning from injury got the necessary hit out, but in any sport the objective is to win. That we did not get and we did not deserve a winning result. There has never been any illusion about the challenge in Super Rugby proper but we will be in a position to field a match squad close to full strength. That wasn't looking possible a few weeks ago.
'It is a credit to our medical and conditioning staff and to the desire of the players.'
Solomons said he was thankful for the commitment of Griquas and how important the match would be in the mental preparation for the historic season opener in a week.
'Griquas approached it like it was a Super Rugby match and it was the physical and mental preparation you can't replicate at training. We've got lots to work with in the next week. But take nothing away from Griquas. They came here to win and they did so.'
The Kings play the Force in Port Elizabeth to start their Super Rugby history.
By Mark Keohane


text:
| David Bailey
J. Michael Cochran, Chairman
Brent Hardwick
Angela Lindstrom (4:04)
Rosa Nowels
John Olmsted
Patrick Robeson, Vice-Chairman
| Robert Hodson, Director of Engineering
Mark Miller, City Traffic Engineer
Don Hoppe, Assistant City Engineer
Dave Langstaff, Traffic Engineering Analyst
Teri Carlson, Senior Traffic Engineering Aide
Lieutenant Cave, Police Department
Sergeant Matson, Police Department
Susan McGraw, Recording Secretary
Chairman Cochran called the meeting to order at 4:00 p.m.
Vice Chairman Robeson made a motion to accept the Minutes of October 6, 2003, with the following corrections:
Page 4, paragraph 3, line 1 should read ". . . doesn't think a no parking regulation will help, and will . . ."
Page 8, line 1 should real "Lieutenant Stanko commented. . ."
Commissioner Hardwick seconded the motion, which passed unanimously.
Traffic Engineer Miller introduced Don Hoppe, Assistant City Engineer, who will be replacing Robert Hodson, Director of Engineering; and Lt. Doug Cave who will replace Lt. Stanko at these meetings.
Mr. Miller presented staff's report. These recommendations are consistent with the Grace Ministries' street striping as required in its development plan.
Commissioner Olmsted bicycled on Valencia Drive this weekend and wondered if there will be bicycle striping as done on Berkeley. Mr. Miller stated that it has been a consideration, and staff will be reviewing this in the near future
Commissioner Bailey questioned the proposed no stopping'. Mr. Miller advised this restriction would not allow parents to drop off their children.
No one was present to speak to this item, so there was no public comment.
Vice Chairman Robeson made a motion to accept staff's recommendations. Commissioner Hardwick made a second, which was unanimously approved.
Mr. Miller presented staff's recommendation. During the planning/striping stage for a proposed car rental business on the south side of Commonwealth, it was discovered that the crosswalk at this location does not meet the established criteria for the installation of a marked crosswalk. Marked crosswalks tend to have a higher rate of accidents than unmarked as the pedestrian tends to have a false sense of security. The vehicle code now addresses notification to the public to remove existing crosswalks. A sign will be posted indicating that the crosswalk will be removed within 30 days.
Vice Chairman Robeson asked if there were any parking issues for the airport. He was concerned for the airport restaurant and inquired if local businesses were notified. Mr. Langstaff advised that notices went to all businesses with no responses received. Mr. Miller advised there are no identified parking issues at the airport.
Commissioner Bailey asked about the legality of crossing midblock at this location without a marked crosswalk. Mr. Miller explained that unless posted otherwise, crossing here would not be considered jaywalking.
Commissioner Olmsted commented that it is a long walk from Magnolia to the restaurant. He inquired who pays to remove the crosswalk. Mr. Miller advised that the City is responsible and this location will be combined with other sandblasting projects.
Commissioner Lindstrom wanted to know who would be liable if there is no marked crosswalk and is there a difference between liability and responsibility. Mr. Miller could not respond without legal interpretation, but questions would be raised if the crosswalk was warranted.
Commissioner Nowels asked if there is any idea why the crosswalk is in that location. Mr. Hoppe stated that possibly there was more business on the south side. Most of the present businesses are located to the west of the marked crosswalk except for an aviation business.
There was no public comment.
Commissioner Olmsted suggested deferring the motion for a month and notifying restaurant business.
Chairman Cochran expressed concern that there is an issue of liability if the crosswalk can't be supported by current warrants.
Commissioner Bailey stated if it is still a legal crosswalk, crossing will be safer. He supported staff's recommendation.
Commissioner Hardwick made a motion to accept staff's recommendation. Commissioner Bailey seconded the motion which passed with Vice Chairman Robeson and Commissioner Lindstrom opposed.
Chairman Cochran opened the meeting for public comment.
Sam White, Ray's Flying Club, asked about the process to place an item on the Transportation & Circulation Commission agenda. He was advised to contact Engineering department staff.
There being no further business, the Transportation & Circulation Commission was adjourned to December 1, 2003.


text:
Best prep performances for the week Dec. 24-29
Glacier Peak | Boys basketball
The Grizzlies guard scored 17 points in a win over Coeur d'Alene as GP won the Coeur d'Alene Inn-Vitational over the weekend. Vader also scored 13 points in a win over Post Falls (Idaho) and 15 in a win over Chief Sealth to earn all-tournament team honors.
Counting the votes: Oak Harbor's Drew Washington won the fan vote. Vader received votes from Herald prep editor Aaron Swaney and Herald prep writer David Krueger. Jackson's Dan Kingma received votes from Herald writers Aaron Lommers and Rich Myhre. Vader won based on finishing higher in the fan voting.
Alyssa Reuble
Meadowdale | Girls basketball
The Mavericks wing averaged more than 10 points per game to earn Most Valuable Player honors as Meadowdale went 3-0 to win the Surf 'N Slam Tournament in San Diego this past week.
Counting the votes: Glacier Peak's Nicole Fausey won the fan vote. Swaney and Krueger voted for Reuble. Lommers voted for Callie Harwood and Myhre voted for Beth Carlson.
Brandon Johnson
Lake Stevens | Wrestling
The Vikings 285-pounder won all six of his matches at The Clash XI to help Lake Stevens go 3-3 at the prestigious wrestling tournament in Rochester, Minn.
Like our high school sports page on Facebook, follow @HeraldNetPreps on Twitter and look for updates on our Prep Zone blog.Story tags » • Arlington High School • Edmonds-Woodway High School • Lynnwood High School • Meadowdale High School • Everett High School • Lake Stevens High School • Snohomish High School • Stanwood High School • Oak Harbor High School • Archbishop Thomas J. Murphy High School • High School Basketball • Jackson High School • Glacier Peak High School
- Prep Zone: Vote for Girls Basketball Player of the Week | Holiday Tournament Edition 12/31/12
- Prep Zone: Vote for Boys Basketball Player of the Week | Holiday Tournament edition 12/31/12
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text:
Michele Wehrwein Albion
On the Bookshelf
Behind the pink tallis: thoughts on Jewish womanhood from Thomas Edison to Gwyneth Paltrow
Comedy Legend Anne Meara Dies6:53 PM —
Logged 36 appearances on The Ed Sullivan Show with husband Jerry Stiller
Bibi Tabs Dore Gold as Foreign Ministry Chief5:29 PM —
Netanyahu tightens his grip
'Jeopardy!' Champ Flubs Jewish-Themed Final Question1:40 PM —
Trebek: 'Whenever people to go temple… they sing Kinky Boots!'
An Interview With the Man Who Made Tevye Sing12:33 PM —
Sheldon Harnick celebrates 50 years of 'Fiddler on the Roof'
Who Was Jon Stewart's Final Guest in 1995? David Letterman10:58 AM —
Letterman: 'Sometimes what you love the most provides you with the most pain'
Obama: Denying Israel's Right To Exist as a Jewish Homeland Is Anti-Semitic6:13 PM —
The president draws a line in the sand in his latest interview
Tablet's Poetic Memorial Day Playlist4:56 PM —
For all those who've died far from their families, and far from peace
How To Protect the Conversion Process3:02 PM —
My friend was recorded in a mikveh by a rabbi. Let's ensure the end of this abuse of power.
Less Dairy, More Davening12:37 PM —
How I observe Shavuot despite my life-threatening allergy
Obama: Denying Israel's Right To Exist as a Jewish Homeland Is Anti-SemiticBy Yair Rosenberg — The president draws a line in the sand in his latest interview
'Jeopardy!' Champ Flubs Jewish-Themed Final QuestionBy Jonathan Zalman — Trebek: 'Whenever people to go temple... they sing Kinky Boots!'
What My Nanny Left MeBy Ross Kenneth Urken — How did a Jewish boy from New Jersey end up speaking with a Jamaican accent? It's an enduring inheritance from a woman who raised me.
No Brisket, No Chopped Liver: Recipes for a Meat-Free Jewish Kitchen
Cruelty & Perversity: Postprandial Reflections on the PEN Protesters
The grim satire of the 'Charlie Hebdo' controversy, in context
Léon Blum: Prime Minister, Socialist, Zionist, Tireless Champion, Proud Jew
How To Protect the Conversion Process
The Chaplain and the Survivors
The Art of Fixing the Failures of Plastic Surgery
Twitter: tabletmag
- "White American Jews *need* to see colour." On Jews and race in America: http://t.co/dR0PlkcMoH
Blum's Day
Sociologist Pierre Birnbaum says it's time Léon Blum—French Socialist, Zionist, wartime hero, and prime minister—got his due
A Lullaby for Auschwitz
On a new album, Italian singer Shulamit offers modern interpretations of haunting songs left by women in the Holocaust
I Was a Teenage Stowaway
Victor Rodack dreamed of visiting Israel. In 1967, he found an unusual way to make his dream a reality.


text:
Rather, the senior middle linebacker is more focused on helping the Bulldogs win the turnover battle, something he said will help
"My individual goal, I'm not as concerned about tackles, but I am about interceptions, forced fumbles and fumble recoveries," Schoenecker said. "Turnovers can change the outlook on a game and that's my goal is to win more games."
The Bulldogs certainly won a lot last year, going 7-4 and 4-1 in the Coastal Plains Conference and advancing to the NCHSAA 1-AA playoffs.
Schoenecker said he had about "65 to 70 tackles" last season, his third year on varsity.
But entering this season, the 17-year-old Schoenecker wants to be one of the defensive leaders.
While he said his position allows him to show his "playmaking ability" because he can "stuff the hole," the 6-foot-1, 189-pound Schoenecker feels he has more responsibility this year on the defensive side.
"I will be calling the plays along with Seth Schoonover," Schoenecker said. "I've called defenses before. I was calling plays when I first started playing football and through rec ball and all the way up through middle school. I've shared the responsibility with Seth, and so I'm really used to it by now."
But there's a difference between calling out the plays in the defensive huddle and being in command as a senior leader. Schoenecker knows he will be looked at as one of the key figures on defense.
"It's definitely an increased responsibility," he said. "As a rising senior, they expect leadership out of you."
And Schoenecker has been doing his part as the Bulldogs prepare for the first official day of practice Aug. 1 and for the season opener Aug. 23 at Spring Creek.
He knows that he can't take the summer off and that the effort he makes during team workouts, practices and in passing leagues will pay off in the end.
"You always have to give everything you have. You have to take workouts and attack them," Schoenecker said. "If you take a day off, your opponents are gaining ground on you."
But there doesn't seem to be anyone who is gaining ground on Schoenecker,
"He just does it," Swaney said. "He is a performer."
Schoenecker certainly performed well in a pair of combines.
Last month, he participated in the Blue-Grey New England Super Combine at the New England Patriots' training complex in
There, he ran a 4.84 in the 40-yard dash, had a broad jump of 9 feet, 4 inches and a 32-inch vertical jump.
Schoenecker was invited to the combine after an impressive showing in the Blue-Grey North Carolina Combine in
"They (N.C. combine organizers) sent out invitations to players and then they chose me to go to New England based on how I performed in
Swaney was not surprised Schoenecker did well.
"We have always felt Bradley was very athletic," he said. "He tests out really well. He's gotten stronger and he's a very intelligent kid. I can't say that I was blown away … because he just does well."
And because Schoenecker has done well this summer, he expects to have a good season.
"You have to have confidence in yourself," Schoenecker said. "Knowing that I performed as well as other people from across the country, my confidence is pretty high."


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Knee Bursitis Center - Roanoke, VA
Roanoke Rheumatologist Doctors for Knee Bursitis
Type of Physician: Rheumatologist
What is a Rheumatologist?
A subspecialty certification by the Board of Internal Medicine; practitioners treat diseases of the joints, muscles, bones, and tendons. The rheumatologist diagnoses and treats arthritis, back pain, muscle strains, common athletic injuries, etc. The rheumatologist may work closely with other specialists such as orthopaedic surgeons and physical therapists.
Specialty: Rheumatology
Common Name:
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Knee Bursitis
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*Provider Directory Terms of Use:
The WebMD 'Provider Directory' is provided by WebMD for use by the general public as a quick reference of information about Providers. The Provider Directory is not intended as a tool for verifying the credentials, qualifications, or abilities of any Provider contained therein. Inclusion in the Provider Directory does not imply recommendation or endorsement nor does omission in the Provider Directory imply WebMD disapproval.


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Ashbrook 6, Highland Tech 3 — Freddie Wells, Josh Rath, Danny Cullum and Blake Ballard won their singles' matches. Daniel Nelli-Rath and Cullum-Sam Pearce won their doubles' matches. Ashbrook (11-1, 5-0) hosts Hickory (ranked fifth in N.C.) on Wednesday.
Varsity baseball
Gaston Christian 15, Piedmont Charter 2 — Tanner Queen (4-for-4, two doubles, four RBI), Landon Guy (2-for-3, two doubles, three RBI) and Strat Guy 1-3 (3 RBI) led Gaston Christian on Monday. Pres Guy, Brandon Conner, Allen Bunton and Blake Cobb also had each had a hit along with a RBI each. Winning pitcher Brandon Conner with Scout Monteith, Pres Guy, and Matt Denham combined for a no hitter. Gaston Christian (5-4, 7-7) host Covenant Day today.
Hunter Huss 10, Highland Tech 3 — Andrew Jordan had seven strikeouts and a walk in seven innings in the Huskies' win. Austin Enloe (1-for-1, three RBI), Connor Anderson (2-for-4, run), Jordan (2-for-3, four runs, four stolen bases) and Zack Splawn (2-for-4, two RBI) led the Huss offense. Guffey (two hits) and Johnny Beard had two hits each. Bronson Short got the loss. Hunter Huss hosts South Point today.
Cherryville 8, East Rutherford 4 — Spencer Stamey (2-for-4, two-run HR), Jonathan Carpenter (2-for-3) and Cameron Fitch (2-for-3, two RBI) led the Ironmen. Alston Mosteller (three strikeouts in four innings) picked up the win. Cherryville (9-10, 6-3) hosts Lincolnton tonight.
Bessemer City 13, Community School of Davidson 2 (5 inn.) — Austin Nixon (2-2) went four innings while allowing two hits for the win and Stephen Clark (1-for-3, double, RBI, run) stood out for BC.
Shelby 8, Forestview 7 (eight inn.)
JV baseball
East Gaston 8, Lake Norman Charter 5 – Nash Jamison (2-for-4, run, stolen base), Christian Huggins (2-for-4, RBI, run, two doubles) and Hunter Mitchem (1-for-4, run, three RBI) led the Warriors. Jamison (two earned runs) picked up the complete-game win with nine stikeouts. East Gaston plays today at Olympic.
Varsity softball
North Gaston 5, Shelby 4 — Haley Hagans picked up the win while Kaycee Bradley (2-for-4) and Chelsea Thompson (2-for-4) led the Wildcat offense. North Gaston plays at Ashbrook today.
Schedule change
Kings Mountain High has made changes to its athletic schedule for the week. The Mountaineers' home baseball games against East Rutherford have been moved up to Wednesday, starting with JV at 4:30 p.m. Kings Mountain's home softball and baseball games against Hunter Huss have been pushed up to Thursday. Baseball is a JV/varsity doubleheader beginning at 4:30 p.m., while softball is at 6 p.m.
Varsity soccer
South Point 3, Hunter Huss 0 — Janet Widehouse had two assists with Hannah Borkowski, Grace Russell and Gabriella Porter scoring goals. Ashley Mull added an assist. Logging the shutout in goal was Constantina Hugg. South Point (8-3-1, 6-1-1) hosts Ashbrook on
Highland Tech 5, North Lincoln 1 -- Kaci McDaniel (three goals), Rachel Terrio (goal, two assists), Monica Rice (goal, assist) scored while Addison Collins and Conner Daniels each had an assist in the win. Jacki Ciokajlo, Katelyn Ford, Karen Fowler, Emily Lopez, Rebecca Rudisill and Conner Daniels stood out on defense. The Rams play at West Lincoln (11-4) on Wednesday.
JV girls soccer
South Point 4, Hunter Huss 0 — Jenna Sarvis (two goals, one assist) and Cora Bridgeman (two goals, one assist) led the offense while Victoria Dalton and Julia Decker shared the shutout in goal for South Point (4-1-1), which hosts Ashbrook on Wednesday.
Middle school baseball
Holbrook 14, Southwest 2 (5 inn.) — Caleb Rhyne, Lito Mantecon and Austin Williamson had two hits and the Lions rolled to a win. Williamson got the win with ten strikeouts. Ryan Rimmer and Hilton led the Roadrunners with one hit and one run each. Holbrook (2-2) hosts York Chester today and visits Friday next Monday; Southwest (1-2) visits Friday on Thursday.
Friday 15, York Chester 0 (4 inn.) — Brae Wooten (three hits) led the team's offense with two hits each from Bradley Propst, Brandon Kang, Alex Owenby and Dillian Parks. Eric Smith picked up the win with seven strikeouts. Justin Jenkins led YC with one hit. Friday (5-0) hosts Southwest on Thursday; York Chester (0-3) visits Chavis on Thursday.
Stanley 7, Grier 3 — Caleb Hager (3-for-3) and Josh Benfield (2-for-2) led the Blue Devils to back Tanner McMinn in the pitching win. Devin Carn (1-for-4) led the Knights. Grier (0-4) hosts Mount Holly (0-4) on Thursday; Stanley (3-1) visits Cramerton on Thursday.
Chavis 15, Bessemer City 1 (6 inn.) — Noah Eaker picked up the win and was 3-for-4 with two doubles. Kameron Reynolds (3-for-3) and Austin Treadway (2-for-3) also stood out for the Wolverines. Chavis (4-0) plays at Southwest on Monday; Bessemer City (3-2) hosts Belmont on Thursday.
Belmont 6, Cramerton 4 — Mason Secrest, Nick Farmer and Grant Tilley combined for the win on the mound. Secrest (3-for-4) stood out on offense while Ethan Broome and Abe Robinson had two hits each. Cramerton (1-2) hosts Stanley on Thursday; Belmont (2-1) visits Bessemer City on Thursday.
Middle school girls soccer
Holbrook 1, Southwest 0 — Savannah Bourgeois scored the goal with an assist from Cheyenne Johnson. Aubrey Marston, Kristin Hunter, and Niyanna Randall led the offense. Holbrook (3-1) visits Friday next Monday; Southwest (0-3) visits Friday on Thursday.
Cramerton 4, Belmont 0 — Ashley Beaty had three goals for Cramerton, with Audrey Emerson scoring the other goal and tallying two assists. Grace Goff and Taylor Johnson each had an assist. Victoria Pelonero, Alexis Altizer and Calee Eisenhuth led the Cramerton defense, while Courtney Conner had the shutout in goal. Cramerton (3-0) hosts Stanley on Thursday. Belmont (1-2) visits Bessemer City on Thursday.
Grier 3, Stanley 2 — Kasen Lindsay and Aeanna Camp scored for Grier, which also benefitted from an own goal by Stanley. Grier (1-3) hosts Belmont on April 23. Thursday's game against Mount Holly was postponed. Rebecca Gomez and Deaja Smith scored for the Blue Devils. Stanley (0-4) visits Cramerton on Thursday.
Friday 3, York Chester 0 — Liz Luksa, Kaitlyn Towery and Katelyn Cochrane scored for the Cavs. Bristol McKee had the shutout in goal. Friday (3-2) hosts Southwest on Thursday; York Chester (2-1) visits Chavis on Thursday.
Chavis 1, Bessemer City 0 — Lauren Gantt scored the lone goal and Courtney Hay had the shutout in goal in the win. Chavis (3-1) hosts Southwest on Monday and York Chester on Tuesday; Bessemer City (1-4) hosts Belmont on Thursday.
Gaston Christian 3, First Assembly 0 — Favour Spears had two goals for Gaston Christian, with Alexis Bean added a goal. Jillien Zukaitis was in goal for the shutout. Gaston Christian (5-4-3) hosts Covenant Day today.
Middle school softball
Gaston Christian 13, First Assembly Concord 2 — Anna Crenshaw (3-for-4) and had four strikeouts in her win on Tuesday. Laramie House (3-for-4, 3-run HR, five RBIs) led the team's offense while Brittany Kernea, Kendal Withers and Jordyn Burrell were all 3-for-4 and had an RBI each for the Eagles.
Chris Barrett of Bessemer City had a hole in one at Catawba Creek in Gastonia on Sunday. Dawson Adams and Nathan Bradley witnessed the ace.


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LEXINGTON, Ky.—Miami University's football team dropped a 41-7 decision at the University of Kentucky in Commonwealth Stadium on Saturday afternoon. The Wildcats improved to 1-1 on the season, while the RedHawks moved to 0-2.
Kentucky quickly jumped out to a 17-0 lead. The Wildcats scored their first touchdown just under two minutes into the game off a Jalen Whitlow 9-yard run. With Kentucky threatening with first down on the 4-yard line, Miami staged a strong red-zone stand and forced the Wildcats' Joe Mansour to kick a 22-yard field goal at the 7:58 mark of the first quarter. Kentucky took a 17-0 lead off a 48-yard touchdown pass from Maxwell Smith to Jonathan George at 5:54.
Miami put its first points on the board when senior linebacker Collin Boucher forced a fumble on a punt return at the Kentucky 6-yard line. Senior linebacker Chris Wade scooped up the ball and plowed into the endzone at the 3:20 mark of the opening quarter. Sophomore Kaleb Patterson's extra point put the RedHawks within 10 points, 17-7.
Just over a minute later, Kentucky's Smith hit Javess Blue on an 88-yard touchdown reception. Mansour's PAT extended the Wildcat lead to 24-7 with 1:58 to go in the first quarter. UK tacked on one more touchdown in the first half, scoring on a Raymond Sanders 5-yard run at 10:10 in the second quarter. Mansour's extra point handed the Wildcats a 31-7 lead, which they carried into halftime.
After a scoreless third quarter, Kentucky added 10 points in the fourth quarter off a 21-yard field goal by Mansour at the 14:15 mark and a 56-yard touchdown pass from Smith to Jeff Badet at 11:53 to push its lead to 41-7.
Senior defensive back Dayonne Nunley led all players with eight tackles, including a game-high seven solo stops.
Miami is idle next weekend before hosting Cincinnati on Saturday, Sept. 21 in the 118th meeting in the Battle for the Victory Bell. Game time will be announced next week. Check MURedHawks.com for the time update.
Notes vs. Kentucky
* Chris Wade returned a fumble for a six-yard touchdown in the first quarter, the first touchdown of his career. The last RedHawk to score via fumble recovery was Craig Mester versus Akron in 2007.
*Fred McRae had a career-long 32–yard punt return late in the second quarter. His previous-high was an 14-yard return last week at Marshall.


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Choose the contemporary Holiday Inn Derby Riverlights hotel for its city centre location and proximity to the Peak District
Holiday Inn Derby Riverlights is situated next to the Intu Derby shopping centre in the heart of the city. Derby bus station is next door and we're a 10 minute walk from Derby Midlands rail station. Flights touch down at East Midlands Airport, 14 miles away. You'll feel relaxed when you step off the bustling street and into our sleek Lobby, with its 4
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Holiday Inn Derby Riverlights is situated next to the Intu Derby shopping centre in the heart of the city. Derby bus station is next door and we're a 10 minute walk from Derby Midlands rail station. Flights touch down at East Midlands Airport, 14 miles away. You'll feel relaxed when you step off the bustling street and into our sleek Lobby, with its 4 storey glass atrium.
We are within 2 miles of your meetings at Rolls Royce and the University of Derby, and a 10 minute drive from Toyota's offices. You can host gatherings and events for up to 120 people in our 4 bright and modern meeting rooms, all of which are air conditioned and have wireless Internet.
Our location in Derby's city centre gives you excellent shopping, dining and entertainment options. You can take a refreshing walk in the countryside after a 30 minute drive to the rolling Peak District National Park. Back at Holiday Inn Derby Riverlights, work out in the MiniGym or unwind in your stylish Guest room.
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GRAHAM – With a lot of need and a lot of existing ministries looking for support, starting a new one is challenging.
Volunteers with DreamAlign are determined they won't fail. The stakes are too high, they said, with so many unmet needs. People need help with food, clothing, school supplies, resources for job searches, and other necessities.
In addition to those physical needs, volunteer Misty London said, sometimes people who are struggling "just need to be loved on."
"It's been an ongoing process of helping families,"
The ministry is expanding in stages, with a full-scale clothing pantry now in place. There are clothes for men, women, teenagers, children and babies.
DreamAlign is collecting clothes people can wear to job interviews. It plans to assist adults with job search and GED efforts while tutoring students.
It helps with emergency food requests and plans to begin a food pantry to offer more regular help.
Volunteers chose Graham, in part, because they thought the city and its residents would benefit from having a ministry there. DreamAlign's office is at
The ministry is working to get the word out that it wants to help. It recently took a flat of spiral notebooks the short haul down the road to
The ministry won't be limited to Graham, however.
"Anyone in
After a lot of work, DreamAlign got its non-profit status last year.
DreamAlign has reached out through events such as a hot dog and candy "No tricks, just treats," event at Halloween and a Thanksgiving food giveaway.
To get involved in Dream Align, or if you need help, call 1-888-559-3373 or visit www.dreamalign.com. At least for now, people needing help are asked to make an appointment. The ministry's building is not always open.
Volunteers hope to share their faith while helping with material needs.
"We're here to stay,"


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The Boston Globe
After 64 years in the heating oil business, Brown's Oil and Air Conditioning of Needham faced a choice as it lost customers to cheaper natural gas: close or sell. Vin Brown, the second-generation owner, chose to sell, merging his company with the larger MacFarlane Energy in Dedham."Financially, it just became a necessity, " Brown said. "Right now natural gas is really doing a number on the oil business."Brown's story is the story of the Massachusetts heating oil industry, which has been shrinking in recent years as fuel prices jumped and natural gas prices plunged. Today, less than one-third of Massachusetts homes — 31.8 percent — heat with fuel oil, down from about 40 percent a decade ago, according to the census.Natural gas heats more than half the state's homes, up from about
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The Eclipse AVN 2210p Honored as Consumer Electronics Innovator At CES 2007
Jan 11 2007
The Eclipse AVN 2210p Honored as Consumer Electronics Innovator At CES 2007
~ The Eclipse AVN 2210p Wins 2007 International CES Innovations Design and Engineering Award ~
Fujitsu-TEN Eclipse AVN 2210p, in collaboration with TomTom, a leading navigation solutions provider, was awarded a 2007 International CES Innovations Design and Engineering Award in the Mobile/Vehicle Electronics Navigation, Safety, and Security, for its custom design plus mobile and entertainment capabilities.
The prestigious Innovations Design and Engineering Awards gives consumer technology manufacturers and developers an opportunity to have the newest products judged by a preeminent panel of independent industrial designers, engineers and members of the trade press.
The Eclipse AVN 2210p featuring TomTom technology, integrates seamlessly with an installed stereo system, and also pops out for fully-functional walking-around duty. Additionally, the AVN2210 has a direct iPod input that will allow control through the TomTom's 3.5-inch color touchscreen. The Eclipse AVN 2210p is the world's first portable navigation device which can be embedded into vehicles through the dashboard or the radio. The unit will be available in the summer 2007.
The TomTom GO and ONE lines of personal portable navigation devices are designed for travel lovers. Whether it's a short drive to the local Dunkin Donuts or a family road trip to Yosemite Park, the TomTom can easily supply a detailed itinerary outlining fast routes, even avoiding toll roads. As the personal navigation market grows and oil prices rise, consumers are looking for "easy to use" devices that can smartly and efficiently direct them to their destination.
TomTom portable navigation devices are available nationwide at retailers including Best Buy, Wal-Mart, Circuit City and RadioShack.
For information or to request a demo unit, contact Sheikina Liverpool at (212) 885-0567 or email@example.com.


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The first major NYC revival of William Inge's powerful mother-son drama Natural Affection is now off-Broadway. Star Alec Beard explains why audiences are in for a surprise when they see TACT's new production of this lesser-known play.
Grace McLean offers some insight into the "gross" and "cute" insects at the center of the off-Broadway musical Bed Bugs!!!October 3, 2014
Almost Home star Jonny Orsini talks about the show's authenticity in portraying a Marine returning to the Bronx in 1965.October 2, 2014
Money Grubbin' Whores star Adam Mucci on the heart, foul language and emotional center of the new off-Broadway play about marriage, divorce and communication.October 2, 2014
Nick Blaemire chats with Broadway.com about the ambitious new musical Found, which tells the story of a man finding his connection to the world through discarded notes and flyers.September 26, 2014
Stage fave Kate Shindle on the absurd universe of the Icelandic tale Revoltion in the Elbow, off-Broadway's only show set in an elbow. Yes, an elbow.August 29, 2014
Qurrat Ann Kadwani brings her Indian parents, her friends and many more to life in her new one-woman show They Call Me Q. Find out why audiences see their own families, as well as themselves, in the 13 characters she plays.August 1, 2014
Between Riverside and Crazy's Liza Colon-Zayas on the hope, humor and New York City realness in Stephen Adly Guirgis' new play.July 22, 2014
Sara Gettelfinger of Atomic discusses what it's like telling the story of the birth of the atomic bomb in this exciting new off-Broadway rock musical.July 16, 2014
Forbidden Broadway's Marcus Stevens on this off-Broadway institution that pokes fun at all things Broadway.June 12, 2014
The Mystery of Irma Vep's Arnie Burton and Robert Sella on the mayhem that ensues at the Lucille Lortel Theatre, where they play all the characters in this hilarious comedy.May 2, 2014
Satchmo at the Waldorf's John Douglas Thompson on the man behind the smile in this one-man Louis Armstrong bio-play.April 18, 2014
Elle McLemore, Jessica Keenan Wynn and Alice Lee of Heathers The Musical on bringing this '80s cult classic to life on stage.April 15, 2014


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Last Updated: Apr 10, 2015
Airplane mode disables your phone's ability to send or receive any type of wireless transmission.
If airplane mode is turned on, your Samsung Galaxy Note® 3 can't connect to the Sprint network.
Note: You can re-enable Wi-Fi and connect to a Wi-Fi network while Airplane mode is enabled.


text: A consultative workshop on the Philippines CBNRM stocktaking exercise was held in Manila in late June of 2011. Over 50 people, representing different stakeholder groups, attended. The morning session included a presentation and discussion of the stocktaking results (both the presentation and discussion are captured in the proceedings). In the afternoon, participants split into 3 working groups to consider (1) technical capacity, (2) policy and institutional mechanisms, and (3) sustainable financing. The principal findings and working group discussions are captured in the proceedings.


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GARETH DUNCAN looks at the Saffas who impressed in Europe over the weekend.
Prop Brian Mujati delivered a strong performance in Northampton Saints' 24-13 win over London Wasps on Saturday. The former Stormers tighthead conceded a penalty in an early scrum, but formed part of a dominating Saints front row thereafter. Mujati was especially powerful in the loose and at the breakdown, and scored a crucial try in the 52 minute by running through flyhalf Nicky Robinson to seal victory.
Saffa-laden Saracens picked up their seventh consecutive win with a 23-10 triumph over Sale Sharks. There were six South Africans in the starting line-up (prop Deon Carstens, hooker Schalk Brits, lock Mouritz Botha, No 8 Ersnt Joubert, scrumhalf Neil de Kock and centre Brad Barritt) while SA-born England prop Matt Stevens came off the bench to score a try.
In the French Top 14, Racing Metro No 8 Jacques Cronje was impressive in a tight 18-12 win over Brive. Cronje toiled through the whole 80 minutes in the match that finished as a kicking contest. Toulouse eighthman Shaun Sowerby had a similar influence in his team's 21-17 win over Perpignan, which saw Kiwi flyhalf Luke McAlister slot six penalties.
In the Pro12 (the former Celtic League), hooker Richardt Strauss and reserve prop Heinke van der Merwe's Leinster enjoyed a 24-19 derby victory over prop Wian du Preez's Munster.


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Enjoy every episode of John Adams.
David McCullough's Pulitzer-prize winning book comes to the screen as a seven-part mini-series.About the Series
Purchase your copy now at the HBO Shop®.The Complete Mini-Series on DVD
George Washington, Thomas Jefferson, Benjamin Franklin -- history comes alive with the characters of John Adams.Cast & Crew
Download the teacher's and student's guides to John Adams now.Teaching John Adams
Get David McCullough's Pultizer-prize winning biography.John Adams: The Book
Enjoy every episode of John Adams. Free with your HBO subscription through participating television providers. It's HBO. Anywhere.®Its John Adams. Anywhere.
Sign up now to get breaking news, fresh content and exclusive deals delivered to your inbox.Drama Series Newsletter


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GasBuddy Blog
Del. G. Manoli Loupassi from Virginia is on a mission to end the practice of Virginia's Department of Transportation assessing a monthly fee on your E-ZPass.VADOT started charging a monthly fee of 50 cents per month on regular transponders and $1 for Flex transponders last July. VADOT insists the fee is necessary to cover administration of the E-ZPass program, which allows users with transponders to use an open road tolling system. Other states also charge monthly fees that vary from 75 cents a month in Ohio to as much as $1.50 per month in Maryland. Indiana, New Jersey, and New York charge $1/mo. Pennsylvania assesses $3 per year.The number of states that charge monthly fees for transponders is limited, and seven states...
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