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Survivor: One World
Exclusive: Kat Edorsson talks about 'Survivor: One World'
By Reality TV World staff, 05/03/2012
Kat Edorsson, a 22-year-old timeshare representative from Orlando, FL, was eliminated from
: One World's merged Tikiano tribe during Wednesday night's twelfth episode of the CBS reality series' 24th edition.
Kat was voted out of her tribe at the season's twelfth Tribal Council session, the sixth session for the tribe since the merge, after her all-female alliance and potential Final 3 tribemates completely blindsided her and sent her packing.
On Thursday, Kat talked to Reality TV World about her
: One World experience -- including what she called her million-dollar mistake, why she defended her "selfish" castaway picks after winning the Reward Challenge, who she admired in the game to the point where she wants to be just like her, what she said was "the worst thing that ever could've possibly happened" to her while on the show, and why she bluntly referred to the family visit as "death."
Reality TV World: Survivor: One World
More Survivor: One World News
Reality TV World: It seems pretty obvious that you were completely blindsided by your vote-off, but why was that? Even if you thought Kim Spradlin and Alicia Rosa were in your Final 3, you were shown saying you did realize there were four other people that were probably going to be upset with you and you probably hadn't made the best strategic choices following your Reward Challenge win. So why wasn't that possibility still in your mind by the time you got to Tribal Council?
Kat Edorsson:
It wasn't still in my head because I was pretty sure that my alliance was very strong and I believed in Kim. Mind you, you have to trust in somebody when you're out there, and I trusted Kim. Don't get me wrong, my million-dollar decision that I threw away was the fact that I chose Alicia for my family visit.
I'm telling you right now, the family visit is death -- I repeat, is death. You don't want to be the picker -- you don't want to be the chooser. You don't want to be the picker. You want to be able to go on the family reward, not choose who goes on the family reward. Because ultimately, no matter what you decide, your decision is going to be wrong. Someone is going to be upset.
Reality TV World: Regarding that, there's been some speculation that Kim intentionally slowed down at the end of this week's Reward Challenge and kind of let you win -- possibly because she didn't want to be in the same position again that she ended up in last week [when you got upset she didn't pick you]. What are your thoughts on that -- do you think she might have intentionally thrown the challenge at the end and done that type of strategic thinking?
Kat Edorsson:
(Laughs) That speculation would be totally false. Kim and I are very much alike where she's old and I'm not, (laughs) no, I'm kidding. But she -- her and I have heart. We have heart for the game, which means there is not one bone in our body that would ever let each other win just because it was strategically.
Kim doesn't do that. She didn't have to win that one. She didn't have to win the reward. She didn't have to win the Immunity Challenge either. She didn't have to. She does it because that's what she loves to do. I do it because that's what I love to do.
And the fact that I didn't win the Immunity Challenge, even if my head was on the chopping block, I still would have held on just as long as I did when I didn't know my head was on the chopping block. So ultimately, me and her, she would never just give me the Reward Challenge ever. I won that fair and square because I was the best and had good communication with my cousin.
Reality TV World: So if Alicia was your million-dollar decision, who do you think you should've taken on the Reward with you instead to change the outcome of the game for you?
Kat Edorsson:
The whole time -- ultimately, it was a really hard decision. I really hated the fact that I had to make that decision. I was fighting between Alicia and [Chelsea Meissner]. I knew that the girls were really upset because everyone was asking me, "Kat, are you kidding?! Why wouldn't you take [Greg "Tarzan" Smith] and his wife? Why wouldn't you take [Christina Cha] and her sick father?"
Don't get me wrong, everybody saw my true colors on the show. I sympathized with everybody, so I completely understand that it would have been less selfish of me to take Christina and Tarzan. However, they would not have benefited me during the game at all. Honestly, they wouldn't.
I told Christina -- if we told Christina, "Vote this way or sit down and stay," she would sit down and stay. If we told Tarzan, "This is what we're doing," that's what we're doing. They didn't make those decisions on their own, so ultimately, I was always going to pick Kim.
My decision was between [Sabrina Thompson], Chelsea and Alicia. Alicia was the worst choice I could have ever chosen, but I was fighting in between Sabrina and Chelsea the entire time when I was making that decision.
It was like, if I took Sabrina, she would not have anything to complain about, and I guarantee if I took Chelsea, she would not have anything to complain about. And the fact that I didn't, they were sour and they were upset, and they made me look like I was selfish. And the day before that, we were all talking about not being like that. (Laughs)
Reality TV World: One of the main reasons the women seemed to vote you out is because they said they were tied of your "childish" and "selfish" behavior and tendency to blame everything on the fact that you're only 22 -- which came across a little weird to viewers, especially since Alicia is only 25, Chelsea's only 26, and Kim and Christina are also still in their 20's. So could you elaborate on that a little bit and share your thoughts?
Kat Edorsson:
Well, I mean, I'm the cute and sweet and goofy one, and if someone's going to blame something on me, you might as well blame it on Kat. Like, "What is she going to do? She's going to be upset for a second and then she's going to smile the next." So, elaborating on the fact that they were upset that I kept blaming it on that I was 22, I don't know.
I think they just needed something to say, honestly. They needed something to say, because honestly, there had to be a reason of why they were eliminating me that day. And ultimately, in the game, they're playing all for themselves. Sabrina was thinking in her head, "Oh, I probably have to get rid of Kat because her relationship with Kim, I don't like it."
And Chelsea felt the same way and Alicia felt the same way. So ultimately, they're going to have to make up a reason why they have to be negative about me, and the only thing that's negative about me is sometimes I can be immature and sometimes I can be naive. But again, I don't think that should've been disclosed as bad as it was last night, but it was. So I just take it and run with it.
Reality TV World: It surprised some viewers that you thought you had a Final 3 alliance with Kim and that the third member of the alliance was Alicia and not Chelsea, who's been presented on TV as being Kim's closest and most obvious ally out there. So what was your take on Chelsea while you were out there and why didn't you seem to recognize that she was Kim's closest ally? Because all the guys that I've talked to that are on the jury have pretty much said it was obvious Chelsea was Kim's "
right-hand man" of sorts out there.
Kat Edorsson:
Right, and it is kind of baffling because of how we can watch it on television right now and I can see it clear as day, but I didn't see it while we were in the game -- very, very easy. Chelsea -- let's see. Kim, we had our Final 3 since Day 1, like Sabrina said.
I thought ultimately it was going to be a better chance for me to win against Kim and Alicia because no one really cared for Alicia at the time because she was being like -- however she was acting, it was Alicia.
And Kim, I had this idea that Kim played hard enough and everybody was so mad at her, that maybe I could win against her. People would think I was sweet. So I thought that was my better option. I would have never took Chelsea. Chelsea, from Day 1, was always my hero. It's hard for people to understand, but when I first met this woman, I was just completely just thrown back by her.
She was someone that I just could imagine -- I would love to be someone just like her when I'm 26 years old -- or that's who I want to be when I grow up. She was just a very inspirational person, but when it came down to the game, we need it really clear not to speak about Final 3, Final 3. Because last night, Kim made it very clear that her best option was me.
Her best option was me. She believed that 100% percent since Day 2. There was never a change. And the whole time, we talked about getting rid of Chelsea and Sabrina all the time, because we could not win against -- I mean, how could I win against Chelsea? She's amazing. Sabrina is amazing. How could I win against them? Kim was my best chance.
Reality TV World: One of the other reasons the women gave for voting you out was because they felt you had close relationships with the men on the jury. It seems like they picked up on that because of Troy "Troyzan" Robertson tapping you on the shoulder and telling you to "do it." Do you feel that was actually the case? Did you have a close relationship with them or were the women just, again, reaching for things in your opinion?
Kat Edorsson:
That was the worst thing that could ever possibly happen to me. I love Troy. He's a silver fox, but he definitely put death on me. That was not a good strategic plan. Everybody saw that -- all the men, yeah they all loved me.
They all thought I was sweet and genuine and I never hurt anybody's feelings. I just kind of went with the game and played as hard as I could. So ultimately, that was him saying from the inside out, "I believe in you. I want you to win." And everybody would vote with Troy, which would ultimately make me the winner.
Reality TV World: How were you cast on
: One World
? How did you end up on the show?
Kat Edorsson:
I was at the right place at the right time and I've always been looking for a challenge like this, and I couldn't ask for anything better than the most incredible game that I could ever play. So I was kind of looking for adventure.
It was one of those times in my life where I was just like, "I need something. I need to do something. I want to make something -- a huge change." And so, it just happened to fall right into my hands.
(Photo credit CBS)
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'Survivor: One World' winner Kim Spradlin gives birth to a son
'Survivor: One World' winner Kim Spradlin pregnant with first child
Sabrina Thompson: I thought I'd win or lose 'Survivor' by one jury vote
Chelsea Meissner: My biggest mistake was not developing better 'Survivor' relationships
Kim Spradlin: I wanted to share 'Survivor' blindsides responsibility with Chelsea and Sabrina
Derek Hough talks condition and whether he can perform on 'Dancing with the Stars'
Kardashian/Jenner family supports Bruce Jenner on social media but Kris Jenner's sincerity gets attacked
E! announces Bruce Jenner's reality series as a transgender woman will debut July 26
Bruce Jenner reveals which children took his desire to become a woman the hardest and the best
'The Amazing Race' engaged couple Matt Cucolo and Ashley Gordon survive non-elimination leg
Bruce Jenner comes out as a woman for the first time, reveals all details of current gender transition
'The Real Housewives of Beverly Hills' star Kim Richards reportedly enters rehab for alcoholism
Linda Thompson addresses Bruce Jenner's gender transition
Colton Cumbie
Jeff Probst
Kat Edorsson
Monica Culpepper
Lady Antebellum's tour bus goes up in flames
Taylor Swift buys porn-site domains with her name
Lilly Collins reportedly dating Chris Evans
Blake Lively a timeless beauty in 'Age of Adaline' posters
'Cinderella' tops weekend movie box office
Jamie Dornan eats his own abs on 'The Graham Norton Show'
Lorena Rojas, Mexican actress, dies of cancer at 44
'It's My Party' singer Lesley Gore dies at 68
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Beyond Playlist presents Xmas-rated, part six: Surprise Packages
This year, we've wrapped our roundup of holiday albums a bit differently. Instead of delivering it to you in one big lump (like coal), we're parceling out the reviews online, with a blog each weekday through December 24 devoted to recordings in a different category. This year's last installment unwraps "Surprise Packages" -- a slew of oddities that don't fit snugly in other categories. After the jump, check out albums by Jim Jones & Skull Gang; Straight No Chaser; various singing groups associated with Princeton University; Peas, featuring Rachel Leslie; and a pair of Chanukah/Hanukkah tie-ins with different ideas about how to spell the holiday in question. To the dictionary!
Straight No Chaser's Holiday Spirits (Atlantic) is the left-field hit of the holiday season: an a cappella offering from former members of an Indiana University singing group that wound up with a major-label deal after a version of "The 12 Days of Christmas" became a YouTube sensation. However, the fact that this tune cross-pollinates the holiday staple in its title with (oh, my God, no!) Toto's "Africa" should give anyone with an allergy to über-shlock pause. I'm sure that when these guys were little, they were No Chasers. Instead, they were the Chased.
A Princeton Christmas 2008, which can be obtained online at PrincetonChristmas.org, is a much more traditional choral effort, and a solid one. The disc features assorted Princeton area singing groups, ranging from the Princeton Day School Madrigal Singers ("Children, Go Where I Send Thee") to the Princeton University Tigerlillies ("On This Day, Earth Shall Sing"). It should appeal to the reverent members of your clan -- and as a bonus, all proceeds benefit the UN World Food Program. That way, purchasers will be helping people in Africa, not the group that sang "Africa."
"Reverence" isn't the word for Mama Doni's I Love Chanukah!, an EP aimed at children whose faith doesn't really make a big deal out of that whole Santa thing (find it at MamaDoni.com). Seven-year-olds will likely enjoy these novelty ditties, exemplified by a rap satire dubbed "The Funky Gold Menorah" and a modified samba with a Ricky Martin twist titled "La Vida Dreidel," while parents will want to keep their distance if they value their sanity. Oy vey.
Erran Baron Cohen Presents...Songs in the Key of Hanukkah (New Line Records) isn't your grandparents' Hanukkah (or Chanukah) album, either. But if the recording's use of modern musical techniques like computerized beats and even a touch of hip-hop is cheeky, it's also very listenable. I particularly enjoyed the wild "Dreidel" and the Rasta-friendly "Spin It Up," both featuring Jules Brookes, and the emotional "A la Luz de la Vela (In the Light of the Candle)," crooned by Yasmin Levy. This is the rare seasonal album that's worth playing during a different season.
Modernization is also the selling point for A Christmas Chill, by Peas, featuring Rachel Leslie (Orange Table). The concept is holiday staples (like "Joy to the World" and "Auld Lang Syne") rendered in the style of club-friendly chill-out fare. Unfortunately, Leslie's vocals have nary a hint of diva to them, and the beats and grooves are burdened with bland production values that fail to capture the quietly ecstatic atmosphere exuded by the best music in this subgenre. I'd call it forgettable if I could remember anything about it.
In contrast, Jim Jones & Skull Gang's Koch Records release Jim Jones & Skull Gang Present a Tribute to Bad Santa Starring Mike Epps will stick in everyone's mind, for better or worse. How zany is this mix of quasi-sentimentality and hard-core rhyming? The intro follows the couplet "Seems chestnuts don't roast on an open fire/Just niggas that'll open fire" with a shotgun blast. Don't expect consistency: The unexpectedly affecting "Christmas in the Ghetto" follows comic Mike Epps's promise to steal your new sweater. Merry Christmas, bitch! -- Michael Roberts
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Thu., April 30, 8 p.m. -
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subset: commoncrawl


text:
Four South Carolina colleges and universities made the The Princeton Review's list of the 150 Best Value Colleges for 2012: Clemson University, College of Charleston, University of South Carolina in Columbia and Wofford College.
The publication selects schools for the list based on excellent academics, generous financial aid and the cost of attendance.
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The Wow Factor
Are you providing the "Wow Factor" in your club?
—The Professional Speaker SeminarProfessional speaker, author and nightclub expert Chris Lenahan gives some "new school" tactics at EXPO 2012 that club owners can utilize to guarantee customers have a truly memorable "Wow!" experience at their club.
For over 15 years, Chris Lenahan has been providing that "Wow factor" at dozens of nightclubs across the country. Lenahan helped design and direct the openings of several Sin City venues, including Club Utopia, Studio 54, the House of Blues, C2K, Light and Coyote Ugly to name a few.
But what if the city you're located in doesn't have the prestige or "cool factor" of Las Vegas? How can you provide that "Wow factor" in your club, no matter what its size and where it's located? EXPO 2012's "The Wow Factor" seminar, presented by Lenahan will tell club operators of all sizes how creating a memorable customer experience has little to do with money, but has a lot to do with having a staff that pays attention to even the slightest detail.
"You literally have 10 seconds to make an impression on your guests, from their first greeting at your front door, to the point at which they're sat down at their table or seat of choice."
"But it's also about having the proper flow," says Lenahan. "You can't give them that 'hard sell' right away; you have to make sure guests are sat in a location they're comfortable with, and make sure they're able to order their drinks and get settled, before the entertainers start coming over to sell dances. It's just like a restaurant, where you are seated, presented with a menu, order drinks, then food, etc. There has to be a flow to the way your guests are greeted and served."
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Gazette polls
Are you going to get a flu shot this year?
Yes | 58% | 214 votes |
No | 39% | 147 votes |
Undecided | 1% | 7 votes |
Total Votes: 368 |
Note: This is not a scientific poll.
September 21, 2012 6:38 a.m. [ Suggest removal ] |
) says...
Why wouldn't you? |
September 21, 2012 4:21 p.m. [ Suggest removal ] |
) says...
Flu shots DO NOT prevent the flu. "For most people, the flu shot does not prevent illness, but actually does the polar opposite--it weakens your immune system and makes you more predisposed to the illness. The people who actually die after contracting the flu do so because they are already sick and have compromised immune systems, and that certainly doesn't have to include you. In addition to making sure your vitamin D levels are in the optimal range, the following tips are surefire ways to improve the function of your immune system and greatly reduce your chances of getting the flu this winter: * Avoid sugar. -Dr. Mercola Be informed. |
September 21, 2012 10:39 p.m. [ Suggest removal ] |
) says...
...As a consequence of the brain damage, vaccines cause you to seek out advice from some half baked physician from the internet....and we all know everything you read on the internet is gospel truth! right Sparkys_Dad? ....oh sorry, he could not respond in a timely manner,because he is alternately getting plenty of exercise digging up Garlic, getting lots of sleep, and running through town terrified trying to avoid sugar while staying informed. |
September 21, 2012 10:41 p.m. [ Suggest removal ] |
) says...
Something gave Sparkys_Dad brain damage, but it wasn't the flu shot. Thanks for the helpful tips like "support yourself...." , whatever the heck that means. |
September 22, 2012 5:12 a.m. [ Suggest removal ] |
) says...
Laughable comments, Sparkys_Dad! When the internet started years ago, the saying was, be careful about getting your medical information off the internet because how do you know it's not coming from your 16 year old neighbor?... answer... you don't! I think the same holds true for most of these medical websites today. |
September 27, 2012 11:41 a.m. [ Suggest removal ] |
) says...
Hey guys-- Check out this site about flu oddities. Very interesting read for sure. |


text:
Fekkai Full Blown Volume Conditioner
Fekkai Full Blown Volume Conditioner is a sheer conditioner that builds up and plumps out fine, flat strand, and works to protect the hair without the weight.
St. Paul, MN
Jul 18, 2013
Great Detangler!
This conditioner does a great job at detangling. I'm not as convinced on its ability to add volume.
2 out of 2 shoppers thought this review was helpful.


text:
Prices and availability are subject to change from the time and date that you print this page.
4/28/2015 4:10:15 AM. For Customer Service, please call
Outlet Item. Available for Shipping Only.
The Story
This classically proportioned, plump pitcher is ready for service of cold refreshers, from ice water and lemonade to margaritas and sangria. Durable clear glass pitcher stands up to everyday use. An ice lip restrains wayward cubes.
Details and Dimensions
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Acapulco Pitcher is rated out of 5 by 40.
Rated 4 out of 5 by Char from Practical & Easy on the eyes I ordered this pitcher for entertaining and I love it. It's practical and easy on the eyes, the only reason I gave it 4 stars is because the glass is thin, so you have to be really careful, but I appreciate the thinness because it's also light. So happy with this purchase!!
Date published: 2015-04-24
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Date published: 2012-10-25


text:
Oregon Ducks Booed at Arizona
Oregon Ducks are booed and attacked with flying debris as they exit the field after defeating the Arizona Wildcats. You stay classy, UofA!
What do you think of this story?
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text:
The use of culture-independent nucleic acid techniques, such as ribosomal RNA gene cloning library analysis, has unveiled the tremendous microbial diversity that exists in natural environments. In sharp contrast to this great achievement is the current difficulty in cultivating the majority of bacterial species or phylotypes revealed by molecular approaches. Although recent new technologies such as metagenomics and metatranscriptomics can provide more functionality information about the microbial communities, it is still important to develop the capacity to isolate and cultivate individual microbial species or strains in order to gain a better understanding of microbial physiology and to apply isolates for various biotechnological applications.
We have developed a new system to cultivate bacteria in an array of droplets. The key component of the system is the microbe observation and cultivation array (MOCA), which consists of a Petri dish that contains an array of droplets as cultivation chambers. MOCA exploits the dominance of surface tension in small amounts of liquid to spontaneously trap cells in well-defined droplets on hydrophilic patterns. During cultivation, the growth of the bacterial cells across the droplet array can be monitored using an automated microscope, which can produce a real-time record of the growth. When bacterial cells grow to a visible microcolony level in the system, they can be transferred using a micropipette for further cultivation or analysis.
MOCA is a flexible system that is easy to set up, and provides the sensitivity to monitor growth of single bacterial cells. It is a cost-efficient technical platform for bioassay screening and for cultivation and isolation of bacteria from natural environments.
Microbe observation and cultivation array (MOCA); Cultivation; Growth; MicrobiotaBackground
During the past over two decades, the use of culture-independent nucleic acid techniques, represented by ribosomal RNA gene cloning library analysis, has unveiled the tremendous microbial diversity that exists in natural environments [1]. In sharp contrast to this great achievement is the current inability to cultivate the majority of bacterial species or phylotypes revealed by molecular approaches. One of the major difficulties for microbial ecology is that conventional cultivation methods provide access to only a very small fraction of the microbial diversity and more than 99% of naturally occurring microbes are considered 'unculturable' on standard culture media [2]. Although recent new technologies such as metagenomics and metatranscriptomics can provide more functionality information about microbial communities [2,3], it is still important to develop the capacity to isolate and cultivate individual microbial species or strains in order to gain better understanding of microbial physiology and to apply isolates for various biotechnological applications [4].
A new view is emerging among microbial ecologists that the majority of so-called 'unculturable' microbial species simply have not been cultured yet. In line with this view, more research is necessary to enhance the ability to culture microbes, in order to reduce the dependence on indirect and cumbersome metagenomic approaches [5,6]. Recent developments in improving traditional cultivation techniques have shown that some conventionally unculturable species can in fact be grown as pure cultures. Tamaki et al. reported that the use of gellan gum instead of agar as the solidifying agent could greatly improve the cultivability of novel microbes on solid media [7,8]. Kaeberlein et al. designed a diffusion chamber to grow previously uncultivated pure isolates of marine origin [9], and the same strategy was also successfully used in cultivation of groundwater microorganisms [10]. Stevenson et al. achieved similar results with soil microbes by fine-tuning the oxygen concentration and nutrient levels [11]. Knowledge obtained through metatranscriptome analysis has also been used in directed cultivation of bacteria [12]. These examples shown that many microbial species can be cultured as long as the environments are optimized for growth.
Recently, some non-traditional cell-isolation technologies have been introduced to isolate targeted cells for pure culture cultivation. For example, Huber et al. used optical tweezers to track and isolate an extremophilic archaeon from a microbial community in a terrestrial hydrothermal vent field [13]. This method of single-cell manipulation provides a new way to grow pure microbial cultures from single mother cells, but it has the disadvantage that the identification and manipulation of the bacteria is an extremely labor-intensive process. By contrast, a high-throughput isolation method has been presented using encapsulated single bacteria in droplets of gel [14], resulting in the successfully cultivation of pure cultures from marine microorganisms. Oligonucleotide probes were used to identify the species after isolation.
One of the remaining hurdles for bacterial cultivation is that fine-tuning the growth condition for any specific species is a daunting effort, especially with the widely diverse microbiota in the ocean and soil environments. Consequently, a high-throughput platform is urgently needed to perform trials of different cultivation conditions in parallel. Previously, several high-throughput microtiter-plate-based cultivation platforms have been developed for marine and aquatic water column bacteria, and these have contributed greatly to the successful cultivation of previously uncultivated bacteria [15,16]. Most recently, a chip-based version of a Petri dish and diffusion chamber has been developed to address the same purpose [17,18]. Microfluidic 'lab-on-a-chip' (LOC) devices have been used for co-cultivation of various bacterial strains and species [19,20]. These devices are complicated in structure, utility, and fabrication, and are therefore less useful for general microbiologists.
In this study, we developed a parallel cultivation set-up that incorporates streamlined processes and is compatible with downstream genomic analysis. It spontaneously isolates environmental bacteria into miniature incubation chambers from a mixed microbial community. The key component of the system is the microbe observation and cultivation array (MOCA), which uses a Petri dish that contains an array of droplets with an oil covering as cultivation chambers. During cultivation, the growth of bacteria across the droplet array can be monitored using an automated microscope, which can produce a real-time growth record. Compared with conventional cultivation methods, MOCA provides streamlined preparation, parallel cultivation, and real-time observation, and unlike other chip-based platforms [16-19], MOCA does not require complicated engineering techniques or equipment for fabrication. We have found that bacterial growth across the droplet array has a high level of uniformity when the initial cell density is more than 10 cells/μl, and thus MOCA provides a novel platform for bioassay screening. When the cell occupancy in droplets is at the single-cell level, real-time image recording can be used to monitor the growth and morphological development of microcolonies derived from single bacterial cells. The droplet culture developed from a single bacterial cell can be transferred using a micropipette [21] for bulk cultivation or further molecular analysis.
Microbe observation and cultivation array
The MOCA Petri dish is produced using a technique known as microscale plasma activated templating (μPLAT) [22,23]. The μPLAT method uses an inexpensive plasma-treatment process to create hydrophilic patterns on Petri dishes (60 mm diameter; Falcon; Becton Diskinson Labware, Franklin Lakes, NJ, USA); the air plasma makes the originally hydrophobic polystyrene surfaces of the Petri dishes hydrophilic.
Briefly, a μPLAT stencil is made from polydimethylsiloxane (PDMS) film, an inexpensive, soft polymer. The stencil has an array of 4 × 6 holes, each 3.18 mm in diameter, which is adhered to the surface of a Petri dish, (Figure 1a). The assembly is placed in a plasma cleaner (Harrick Plasma, Ithaca, NY, USA), and exposed to air plasma for 1 minute (Figure 1b). The stencil is removed after plasma treatment. Because only the polystyrene surface under the circular openings is exposed to the plasma, these areas become hydrophilic on the hydrophobic background of the plate (Figure 1c). The patterned MOCA Petri dish is then surface-sterilized under UV light for 15 minutes in a PCR cabinet (PCR Workstation; Thermo Fisher Scientific, Hudson, NH, USA). The bacteria and medium mixture is loaded using a pipette into the prepared Petri dish, producing a 4 × 6 array of 1-μl droplets. The borders of the liquid are confined at the hydrophilic/hydrophobic interface, forming discrete droplets defined by the stencil pattern. To prevent evaporation and contamination from the ambient environment, 5 ml mineral oil (M5904; Sigma Chemical Co., St Louis, MO, USA) is loaded to cover the droplet array (Figure 1d).
Figure 1. Scheme of microbe observation and cultivation array (MOCA) droplet preparation. (a) A stencil with an array of holes is adhered to a Petri dish, which is then (b) exposed to air plasma to generate an array of hydrophilic disks. (c) The stencil is then removed, and the medium containing the bacteria is added to the hydrophilic disks, after which (d) the droplet array is covered with mineral oil.
Loading of bacteria in medium and culturing using microbe observation and cultivation array
Luria broth (Difco; Becton, Dickinson and Company, Sparks, MD, USA) was used for both bulk cell and droplet cultivation of the gram-negative bacterium Escherichia coli DH5α and the gram-positive bacterium Bacillus subtilis 168. Marine broth (Difco' Becton, Dickinson and Company) was used for droplet cultivation of marine bacteria from sea water sampled from the deep sea of the northwest Pacific Ocean [24]. Cells were counted using a counting chamber (Hausser Scientific Partnership, Horsham, PA, USA) under a microscope to estimate the bacterial density for both pure cultures and sampled sea water. Four cell concentrations (103, 102, 10, and 1 cell(s)/μl) were used in the experiments. Cell occupancy in the droplets is a Poisson random variable [23]; when 1 cell/μl medium is loaded into the 1 μl droplets, the average cell occupancy is one cell per droplet.
For each individual hydrophilic MOCA spots, 1 μl bacteria-medium mixture was pipetted onto it. The droplets were then covered with 5 ml of mineral oil and the lid placed on the Petri dish. The dishes were kept in the dark at room temperature, and growth was monitored by direct microscopy.
Automated time-series observation
A MOCA dish can be monitored on an automated optical microscope to observe the growth of the loaded bacteria. The MOCA Petri dish was placed on the automated microscope (Eclipse TiE, Nikon Instrument Inc., Japan) with a 4× objective, so the field of view covered an entire droplet (Figure 2). The microscope stage was programmed to travel through all the droplets and to acquire bright-field images of all droplets every 30 minutes for 72 hours. The microscope illumination was turned on only during image acquisition to avoid heating of the dish. After the experiments, the time-lapse micrographs of each droplet were used to estimate bacterial growth using measurement of light transmission; the micrographs appeared darker as bacteria grew. Although an automated microscope was used in this study, other optical platforms (for example, conventional microscopes or even the naked eye when the droplets are saturated with microbes) can also be used for estimation.
Figure 2. Demonstration of automated time-series observation by optical microscopy of bacterial droplet cultivation using microbe observation and cultivation array (MOCA).
Phylogeny analysis of isolated bacteria from single cells
The cultivated isolates developed from single bacterial cells of interest can be transferred using a micropipette from the MOCA chip to other containers for archival and downstream phylogenetic analyses using 16S rRNA sequencing. In this study, bacteria were removed from the droplets using a micropipette and transferred to a 1.5 ml microcentrifuge tube containing 100 μl sterilized water. After being spun in a centrifuge at 12000 g for 1 minute, the supernatant was decanted to remove possible carry-over of the mineral oil, then the bacterial cells were re-suspended in 5 μl sterilized water and transferred to a 0.2 ml PCR tube. The PCR tubes were then put in a PCR thermal cycler (model 9700; Applied Biosystems Inc., Foster City, CA, USA) and heated at 95°C for 5 minutes, followed by chilling on ice to induce cell lysis. Using heat-treated bacterial cells as the template, conventional PCR was carried out to amplify the highly variable V6 region of the small subunit (SSU) rRNA gene. The PCR primer sequences are shown in Table 1[25]. The 20 μl PCR volume contained: 10 μl 2 × PCR Master Mix (Fermentas Life Science Inc., Glenburnie, MD, USA), 2 μl of each primer (4 μmol/l), 2 μl of DNA template and 4 μl of double-distilled water. The conditions for PCR amplification were as follows: 94°C for 5 minutes; 35 cycles of 94°C for 15 seconds, 56°C for 20 seconds, and 72°C for 45 seconds; with a final extension step of 72°C for 10 minutes and soak at 4°C. The expected PCR products were recovered with a gel and extracted (Qiaquick Gel DNA Extraction Kit; Qiagen Inc., Valencia, CA, USA). The purified PCR products were used as templates for DNA sequencing, using a commercial kit (BigDye Terminator Cycle Sequencing Kit, version 3.1; Applied Biosystems Inc.) and a PCR analyzer (model 3700; Applied Biosystems Inc.) using the same PCR primers as before. The resultant DNA sequences were edited manually using Sequence Scanner Software (version 2.0; Applied Biosystems Inc.). Sequences were compared against those in GenBank through the National Center for Biotechnology Information (NCBI) portal and BLAST software (version 2.2.10) [26], and the phylogeny affiliation of each isolates were then inferred.
Table 1. Primers used for PCR
Results and discussion
Bacterial growth in droplets
Because MOCA droplet settings create a different physical environment from that of bulk cultures, this may influence many of the growth factors required by the bacteria [27,28]. Thus, given that the total volume for cultivation is a microliter, bacterial growth within the droplets may have different properties from that in regular cultures. To test if the droplets could inhibit bacterial growth, we initially cultivated E. coli and B. subtilis cells within droplets with a cover of mineral oil and assessed their growth in real time. Using the method described above, a 4 × 6 array of 1 μl droplets for each species were generated for the four cell concentrations (103, 102, 10, 1 cell(s)/μl), using six droplets for each cell concentration. Cell occupancy in the droplets is a Poisson random variable [23]: when loading 1 cell/μl medium into droplets, around 37% of droplets are empty, and 37%, 18%, 6%, and 1.5% of the droplets are occupied by one, two, three, and four cells, respectively. Therefore, the lowest cell concentration in all presented cultivation experiments yielded single-digit bacterial cells in droplets.
Figure 3 shows the growth of these two bacteria, where each curve represents the growth in an individual droplet. We measured light transmission through the acquired micrographs as an indication of cell density; the lower the transmission, the larger the cell population. The transmitted light intensity was normalized with respect to its initial value to reduce the light variation between droplets., therefore, all growth curves started from 1. The colors of the curves in the figure indicate initial cell concentrations. The curves of the individual cell concentration were clustered before reaching the stationary phase, with a few outliers appearing for droplets with low initial cell concentrations. We defined the threshold growth time as the time when the normalized transmission intensity of a droplet intersected 0.99 (Figure 3, red dotted lines). The box plots (Figure 3b,d) show the threshold growth time, which was mostly linear to the individual cell concentrations. Because of random cell occupancy and cellular heterogeneity, the variation was large when bacterial number within the droplets was in single figures.
Figure 3. Real-time growth record of bacteria in microbe observation and cultivation array (MOCA). (a,c) Growth curves of (a) Escherichia coli and (c) Bacillus subtilis with different initial cell concentrations (the lower the transmission, the larger the cell population). Each curve represents the growth in an individual droplet. (b,d) Threshold growth time distribution versus (b) E. coli and (d) B. subtilis cell concentrations. The threshold growth time was defined as the time when the normalized transmission intensity of a droplet intersected 0.99 (Figure 3a and 3c, red dotted line).
The growth curves of both E. coli (Figure 3a) and B. subtilis (Figure 3c) shared similar features. The curves of individual cell concentration were clustered before growth reached the stationary phase. One distinct feature of the B. subtilis growth is that the transmission intensity became brighter after the population peaked. The time-lapse micrographs showed that the number of cells did not significantly change, but the size of cells became smaller, suggesting that the B. subtilis cells started to spore after the nutrition was depleted [29]. Only two of the most diluted droplets contained B. subtilis cells that were able to grow into colonies in this experiment. Similar to the E. coli cultivation result, the threshold growth time was mostly linear to individual cell concentrations (Figure 3d), whereas the lowest concentration had a large variation because only two droplets resulted in growth.
These results show that, when the initial cell density is more than 10 cells/μl, bacterial growth across the droplet array is strongly uniform and reproducible. For droplets with single-cell occupancy, the platform can be used to monitor the growth heterogeneity at the single-cell resolution.
Morphological development of microcolonies within droplets
We found that the droplet setting permitted the growth of both E. coli and B. subtilis. In addition, we also found that the bacterial growth pattern within a droplet was different from that of a regular liquid medium. This was especially evident when cultivating single-digit numbers of bacterial cells within a droplet. Using the time-series micrographs of the 24 droplets with E. coli growth recorded at 0, 8, 16, and 24 hours (Figure 4), we found that each single E. coli cell could develop its own microcolony, and these did not merge with each other until the solution became cloudy. Similar results were seen for B. subtilis (data not shown). In this respect, the growth pattern of both E. coli and B. subtilis within a droplet is more similar to that on a solid agar plate than in a liquid medium. This may due to the use of the oil droplet cultivation platform, in which the physical environment is different from that of routine liquid cultivation. For instance, oil coverage may create a microoxic environment, which may inhibit or reduce the movement of bacteria [30].
Figure 4. Proliferation of Escherichia coli in droplets. The time-series micrographs of 24 droplets at 0, 8, 16, and 24 hours. The dark clusters in the droplets are E. coli colonies. For all four panels, the nominal cell numbers of the five rows are 104, 103, 102, 10, and 1 cell(s) per droplet, respectively.
Environmental bacteria cultivation and isolation using a microbe observation and cultivation array
We further used MOCA to cultivate and isolate environmental marine microbes, which were sampled from the Hydrate Ridge site (80 km off the Oregon coast and 5 m above the 780-m deep seafloor) during a research cruise between 22 July and 5 August 2008 [24]. To avoid loading multiple cells from each droplet, the cell concentration was diluted to 0.25 cells per droplet with marine broth medium. Based on Poisson statistics, the possibility of multicell occupancy with this concentration is around 2% and that of single-cell occupancy is 20%.
Using the automated time-series observation system, we could identify which droplets had bacterial growth and how many microcolonies had been developed within these droplets. Droplets showing single microcolony growth were identified and the cultivated pure microbes recovered directly. For verification of purity, the recovered cultures were subjected to further purification on traditional Petri dishes.
In one experiment, thirty-six droplets were generated, and eight of these were successfully cultivated during a 2-day culture period. Time-lapse micrographs showed that they were all derived from a single bacterium (data not shown). The profiles of the growth curves showed similar trends to those of the previous results of the single-digit bacterial cell growth in a droplet (Figure 3), but the variation was larger, perhaps due to species differences or to the different time requirement for exit from dormancy status (Figure 5). Some fast-growing droplets (for example, those in droplets 8 and 9) started growing within the first 8 hours, whereas others (for example, droplet 21) started as late as the 30th hour. The threshold growth times of these droplets are listed in Table 2.
Figure 5. Growth curve of marine bacterial isolates within the droplets. Each curve represents the growth in an individual droplet. The number in the legend is the droplet number associated with Table 2.
Table 2. Cultivated bacteria, their phylogeny affiliation and corresponding threshold growth times
Single colonies were identified in these droplets and the transferred cells were subjected to colony PCR. After sequencing the obtained PCR products of 400 bp, which represent the highly variable V6 region of the SSU rRNA gene, a phylogeny analysis was conducted based on NCBI database searches. Sequences were deposited in GenBank under accession numbers JQ178347 to JQ178354. The closest species/genus with which each isolated strain was identified are listed in Table 2. Of these identified cultures, the majority (six out of eight cultures) belonged to Pseudoalteromonas spp. (E value = 0), with the remaining two identified as Shewanella sp. (E value = 0) and Colwellia piezophila (E value = 0), respectively. The phylogeny affiliation of recovered isolates is consistent with the source of the microbial community [31-33].
Because MOCA droplets create a different physical environment from that of bulk cultures, , this may influence many of the growth factors required by bacteria. For example, the use of an oil cover reduces gas exchange with the ambient environment, which may favor the growth of microaerobic bacteria, but not strict aerobic bacteria. In addition, putting MOCA into an anaerobic chamber and loading with a bacteria-media mixture under anaerobic conditions can allow an anaerobic environment to be created. We will explore the potential of this technique and its limitations in cultivating novel environmental bacteria in future studies.
We have developed a new system to cultivate bacteria in a droplet array. Our results show that the set-up of droplet does not inhibit the growth of bacteria, as we found that both E. coli and B. subtilis cells could proliferate uniformly across the MOCA. We also found that the bacterial growth pattern within a droplet is more similar to that on a solid than in a liquid medium. Given its flexibility, ease of set-up, and sensitivity to growth of a single bacterial cell, we believe that this droplet platform could provide a cost-efficient way to cultivate and isolate novel bacteria from different environments, and had the potential for use in bioassay screening.
MOCA: Microbe observation and cultivation array; DNA: Deoxyribonucleic acid; rRNA: Ribosomal ribonucleic acid; SSU rRNA: Small subunit ribosomal RNA; PCR: Polymerase chain reaction; μPLAT: Microscale plasma activated templating; PDMS: Polydimethylsiloxane.
Competing interests
The authors declare that they have no competing interests.
Authors' contributions
WG conceived of the study, participated in its design, carried out the phylogenetic studies, and was involved in the interpretation of all data. DN and JN carried out the preparation, cultivation processes, and phylogenetic studies. WZ conceived of the study, participated in its design, and was involved in the interpretation of all data. SC conceived of the study, participated in its design, and analyzed the results. DM conceived of the study. All authors read and approved the final manuscript and were involved in the interpretation of data.
We thank the crew of the R/V Thompson TN221 Research Cruise with chief scientists Dr John Delaney and Dr Deborah Kelley of the University of Washington, and we thank Dr Cody Youngbull at Arizona State University (ASU) for the help with the water sampling, and the staff in the ASU DNA laboratory for providing DNA sequencing service. We also thank ASU for the support of this research. DN and JN appreciate the high-school summer internship program of the Biodesign Institute at ASU.
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Nanoscale patterning using self-assembled polymers for semiconductor applications


text:
A Saskatchewan newspaper recently published a letter sent to them by Guelph MP Frank Valeriote. The letter accuses MP Andrew Scheer of choosing to "make significant cuts to the Canadian Food Inspection Agency by voting in favour of the Conservative omnibus budget bill."
The problem for Valeriote is that this did not happen. Scheer did not vote on this bill. Why? Because he is the Speaker of the House of Commons and only votes in the event of ties. This bill passed handily.
Canada has been fortunate over the years (compared – most notably – to some of our Commonwealth brethren) of benefiting from a continuous series of outstanding, non-partisan house speakers- both Conservative and Liberal. By all accounts Speaker Scheer continues this tradition.
I don't profess to know the motivation for Valeriote to have such a factually incorrect letter published in the Speaker's riding but this is a partisan attack on the integrity of the Speaker himself.
It is very disappointing that Guelph's MP conducts himself in such a manner. I strongly suggest that Mr. Valeriote apologize to Scheer and send a retraction to the newspaper in question.
Marty Burke
Clear -1° C | Weather Forecast


text:
Effects of monomer/liquid crystal compositions on electro-optical properties of polymer-stabilized blue phase liquid crystal |
Optical Materials Express, Vol. 1, Issue 8, pp. 1502-1510 (2011)
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A polymer-stabilized blue phase liquid crystal is one of the candidates for the next-generation display material. In this study, effects of monomer/liquid crystal compositions on electro-optical characteristics of polymer-stabilized blue phase liquid crystals were investigated. By optimizing monomer/liquid crystal compositions, lower operating voltage, reduction in hysteresis and residual birefringence were achieved. However, some technical issues remain to be overcome.
OCIS Codes
(160.3710) Materials : Liquid crystals
(160.5470) Materials : Polymers
(230.2090) Optical devices : Electro-optical devices
ToC Category:
Liquid Crystals
Original Manuscript: October 12, 2011
Revised Manuscript: October 18, 2011
Manuscript Accepted: October 18, 2011
Published: November 4, 2011
Virtual Issues
Liquid Crystal Materials for Photonic Applications (2011) Optical Materials Express
Thet Naing Oo, Tatsuro Mizunuma, Yasutomo Nagano, Hengyi Ma, Yukiko Ogawa, Yasuhiro Haseba, Hiroki Higuchi, Yasushi Okumura, and Hirotsugu Kikuchi, "Effects of monomer/liquid crystal compositions on electro-optical properties of polymer-stabilized blue phase liquid crystal," Opt. Mater. Express 1, 1502-1510 (2011)
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- D. Kubota, T. Ishitani, A. Yamashita, S. Yamagata, Y. Oe, T. Tamura, M. Ikenaga, T. Yamamoto, M. Kato, M. Nakano, R. Hatsumi, Y. Kubota, T. Murakawa, M. Hayakawa, T. Nishi, S. Seo, Y. Hirakata, S. Yamazaki, K. Okazaki, R. Sato, T. Cho, and M. Sakakura, "A new process for manufacture of low voltage, polymer-stabilized blue phase LCDs," SID Int. Symp. Digest Tech. Papers42(1), 125–128 (2011). [CrossRef]
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Categories:NewsVolunteer OpportunitiesEmployee ResourcesPerforming ArtsEventsTell Your Students |
UNI Online is a compilation of notices distributed to faculty and staff. The deadline for submission is noon, the day before publication. UNI Online is distributed on Tuesday and Thursday mornings. Submitted items must be sponsored by a UNI department, program or organization. No commercial or personal announcements are allowed. Announcements must have news, not opinion content. Submit items to: http://www.uni.edu/unionline/ |
Thursday, November 10, 2005NEWSIHSAA FOOTBALL PLAYOFFS - EXPECT TRAFFIC CONGESTION:There will be four Iowa High School Football Playoff games in the UNI-Dome Friday, Nov. 11. This will mean increased traffic on Hudson Road, and parking may be affected. Friday's games include: 10:30 a.m., Regina, Iowa City vs. Dike-New Hartford 1:30 p.m., St. Albert's, Council Bluffs vs. Bishop Garrigan, Algona 4:30 p.m., Cedar Falls vs. Assumption, Davenport 7:30 p.m., Valley, West Des Moines vs. Dowling Catholic, West Des Moines. See the complete playoff schedule at http://www.vpaf.uni.edu/unidome/IHSAAPlayoffs.htm ATTENTION MEMFIS USERS:Due to security concerns, effective Saturday, Nov. 12, we will begin blocking access to the production MEMFIS system from many Internet service providers. We will allow all of the common providers in this area to continue to access our production system, including those that any UNI satellite offices use. For additional details, visit: http://www.uni.edu/unionline/public/412.html UNI VISITOR CENTER:Since the UNI Visitor Center opened in August, most inquiries received relate to parking. The Visitor Center does not sell visitor parking passes. Guests are directed to the visitor lot or campus meters. If departments wish to provide parking permits for campus guests, contact UNI Public Safety. If departments want the Visitor Center to distribute those permits, contact email@example.com or e-mail firstname.lastname@example.com Visitor Center hours are 8 a.m. to 5 p.m., Monday through Friday. WINTER OPERATIONS:The Facilities Services staff will begin switching mechanical systems in all major buildings to winter operations beginning Monday, Nov. 14. Building temperatures could run warmer than normal depending on outside temperatures. Questions regarding how your building will be affected should be directed to the area mechanic assigned to your building. AWARD NOMINATIONS BEING ACCEPTED:Nominations are now being accepted for Administration and Finance Division's new High Five Award for Staff Excellence. Any A&F employee is eligible to receive the award, and any member of the UNI community may submit a nomination. Check your campus mail for more information and nomination form, or go to http://www.vpaf.uni.edu/recognition/ and click on the link for "High Five." For more details, call Robin at 3-3536. INTERNAL SEARCH:The Provost Office invites applications for the position of Director of Academic Assessment. Details and application procedures are posted at http://www.uni.edu/vpaa/ under "Internal Search." All eligible faculty and staff are encouraged to apply. The application deadline is Monday, Nov. 21. ORDER HOLIDAY PIES:Try delicious new pies available by the slice at Prexy's. Orchard Fruit with vanilla crust, Wild Berry Blast with lemon crust and more. Inquire about purchasing whole pies for your holiday gatherings. RATE DINING SERVICES:Give us your opinion of UNI Dining Services this week by completing an online survey at http://www.NACUFScustomersurvey.com Use "124(followed by your six-digit UNI ID number)" as the logon code. You may rate one or more dining venues and you may log on multiple times. VOLUNTEER OPPORTUNITIESFOOD BANK VOLUNTEERS NEEDED:The Northeast Iowa Food Bank is seeking food room/salvage volunteers to flatten sacks, put meals sacks together and sort food. Hours are flexible. Groups and individuals welcome. Commitments can be one-time or long-term. E-mail firstname.lastname@example.com for more information. EMPLOYEE RESOURCESFREE MEMORY SCREENINGS:Stop by Maucker Union's Meditation Room Tuesday, Nov. 15, between 9 a.m. and 5 p.m., to learn about the resources available in the Cedar Valley to help people living with dementia or caring for someone who is. Free, confidential, baseline memory screening will be offered to anyone interested. Contact firstname.lastname@example.com or call 3-7961 for more information. Appointments preferred. SPECIAL DELL COMPUTER PRICING AVAILABLE:New Dell personal computer systems pricing for departments and students/faculty/staff, are available at http://www.uni.edu/its/us/purchase This pricing is due to combined purchase estimates from UNI, ISU and UI. Some configured systems, which can be modified to suit your individual needs, are available along with the entire Dell line. IRISH AND SCOTTISH DANCE LESSONS:Learn Irish and Scottish social dances Sunday, Nov. 13, at 5 p.m., in the Maucker Union Old Central Ballroom. Admission is free. Visit http://www.uni.edu/dance/ceili.htm for more information. PERFORMING ARTSJAZZ CONCERT:On Saturday, Nov. 12, David Binney and the Jazz Quintet will perform in the Russell Hall Auditorium at 8 p.m. OUR TOWN:Theatre UNI will present Thornton Wilder's Pulitzer Prize-winning drama "Our Town," Nov. 11-13 and Nov. 17-20 at the Strayer-Wood Theatre. Tickets are $12 for adults, $8 for students and free for UNI students with UCard. Tickets may be purchased at the Strayer-Wood Theatre box office, 273-6381 or online at: http://www.theatreuni.com OPERA ENSEMBLE:On Monday Nov.14, at 8 p.m., an opera ensemble will showcase scenes from "The Medium" and selections from Russian operatic repertoire, in the Russell Hall Auditorium. Admission is free. HORN CHOIR RECITAL:Thomas Tritle will conduct a brass program Tuesday, Nov. 15, at 8 p.m., in GBPAC Davis Hall. Admission is free. UNITUBA ENSEMBLE:The UNITUBA ensemble will perform, at 8 p.m., Wednesday, Nov. 16, in GBPAC Davis Hall. EVENTSCAPOEIRA CONFERENCE:The Capoeira Conference will take place Nov. 10 through Nov. 13, with a show Saturday, Nov. 12, in the West Gym, from 7 to 9 p.m. Come watch professional dancers, martial artists and acrobats perform this Afro-Brazilian art form along with students from the Midwest. Free admission. Capoeira workshops will be offered at the YWCA in Waterloo all day Friday, Nov. 11, and Saturday, Nov. 12, and also at the CME on Sunday, Nov. 13. For more information, contact firstname.lastname@example.com, 319-290-5503 or visit: http://www.brazilianmartialarts.org PANTHER ATHLETICS:Thursday, Nov. 10, women's basketball vs. Winona State (exhibition) in the West Gym at 7:05 p.m. INTERNATIONAL EDUCATION WEEK:UNI will join universities nationwide to celebrate International Education Week, Nov. 14-18. This week-long celebration includes: language lessons, salsa, African and belly dance demonstrations/lessons, live music performances, Peace Corps presentation, Native American speakers and more. For more information, visit: http://www.uni.edu/studyabroad/news/IEW2005.asp GOLD STAR FAMILIES FOR PEACE:Al Zappala, the father of a fallen Iraq war veteran, will speak on campus at 1 p.m., Friday, Nov. 11, in the Maucker Union Old Central Ballroom. Sponsored by UNI Students for Social Justice and Cedar Valley United for Peace and Justice. For more information, contact Chris Schwartz at firstname.lastname@example.com Free and open to the public. INDIAN NATION DANCE PERFORMANCE:The Haskell Indian Nation University Dance Performers will appear Friday, Nov. 11, from 11 a.m. to 12:30 p.m., in Maucker Union Old Central Ballroom. Dessert and pop provided. The performers will be dressed in traditional dance regalia and will explain, and then demonstrate, their unique inter-tribal dance style. For more information, visit: http://fp.uni.edu/cme/nov.htm EARTH SCIENCE SEMINAR:On Monday, Nov. 14, Professor Marcia Bjornerud from Lawrence University, will present "Words, Maps, Snow, Rock: One Geologist's Unlikely Path to Science and Science Writing," at 4 p.m., in LAT 125. All are welcome. Bjornerud is author of "Reading the Rocks," an assigned reading in 870:031 (Physical Geology). LAST LECTURE SERIES:The final "Last Lecture Series" presentation of the semester will take place Tuesday, Nov. 15, at 7:30 p.m., with James Robinson, Department of Philosophy and Religion, presenting "History, Religion & Humanity: A Summary of 30+ Years of Teaching," in the Seerley Hall Great Reading Room. The series invites faculty to present a lecture on any topic as if it were their last. Free beverages and snacks provided. CIVIC DISCOURSE AND OPPOSING VIEWS:Tuesday, Nov. 15, from 4 to 5:30 p.m, in SEC 252, participants will experience arguing both sides of an issue and then engage in discussion with members of a panel. No preparation required. Talking points will be provided for both sides. The issue for the first session is "Should there be a draft?" Free and open to the public. RSVP to firstname.lastname@example.com (type "opposing views" in subject line). ROY R. BEHRENS LECTURE:UNI professor of art and the CHFA Faculty Excellence award winner, Roy R. Behrens, will present "Teaching as a Subversive Inactivity," at 7:30 p.m., Tuesday, Nov. 15, in the CAC, Room 108. A reception will follow. For more information, contact Ivy Walker or Roy Behrens at email@example.com NATIVE AMERICAN HERITAGE MONTH - KEYNOTE SPEAKER:Elizabeth Cook-Lynn will speak Tuesday, Nov. 15, at 7 p.m., in Lang Hall Auditorium. A reception and book signing will follow. Cook-Lynn is one of the writers of the 20th century Native American Literary Renaissance and is the author of three novellas, short stories, and poetry. Her books include "Why I Can't Read Wallace Stegner and Other Essays: A Tribal Voice" and "The Politics of Hallowed Ground". For more information, on Cook-Lynn and NAHM events visit: http://fp.uni.edu/cme/nov.htm PAT/HISTORY CLUB LECTURE:On Wednesday, Nov. 16, at 7 p.m., in SRL 115, Jeff Kurtz, executive director, Cedar Falls Historical Society, will present "The Cedar Falls Historical Society and the UNI Department of History: A Developing Partnership." The event is free and open to the public. WAL-MART WATCH FORUM:Sweat shop workers from four continents will speak on Wal-mart's labor rights policies Wednesday, Nov. 16, at 7 p.m., in Sabin Hall Room 102. Hear about their battles to win basic rights such as unions and a living wage. For more information, contact Laura Greenwood at email@example.com TELL YOUR STUDENTSCAPSTONE COURSE FOR SPRING:Gretta Berghammer is offering Theatre in Education (490:106: 3 hrs; prerequisite: junior standing) in the spring. This course is available to all majors and satisfies the Liberal Arts Core Capstone requirement. STUDY ABROAD IN SPAIN:AIFS will offer a free round-trip airline ticket for fall 2006 programs at the University of Salamanca and the University of Granada in Spain. Applications must be postmarked by Apr. 1, 2006. For more information, contact: firstname.lastname@example.com or visit Baker Room 59 or phone 3-7078. Online information available at: http://www.uni.edu/studyabroad Find the UNI event you're looking for in the UNI Calendar of Events, a convenient, searchable database located at http://access.uni.edu/acal/Events on the calendar this week at UNI: http://access.uni.edu/acal/bin/callist.cgi?date=this_weekTo submit an item to UNI Online: Can't link to the items listed? Have a question? Visit our FAQ site: UNI Online Archive UNI Online Editor The University of Northern Iowa offers a world-class university education, providing personalized experiences and creating a lifetime of opportunities. |
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Current Events & Hot Topics
Featured Posts
SALT LAKE CITY — A new bill being considered in the Utah State Legislature would let people get guns out of their homes to avoid incidences of gun violence.
Rep. Dixon Pitcher's HB121 creates a "safe harbor" where people can voluntarily surrender their firearms to local police for up to 60 days.
"You can deposit the gun, short term, to get through an emergency," said Pitcher, R-Ogden. "That's what the safe harbor bill does for firearms."
Pitcher said police would register the gun and keep it safe.
"It provides a platform if there's emergencies in a family — let's say there's somebody who feels like they're suicidal or they have someone with a mental illness or a mental dispute or some type of emergency," said Pitcher. "Under the conditions we have right now, this is a good time to get the firearm out of the house."
Pitcher said the bill came out of the aftermath of the Sandy Hook Elementary shooting. The Ogden lawmaker wondered if circumstances could have changed if the shooter's mother had been able to get her guns out of the home, like his bill would do.
"It saves lives," he said.
Clark Aposhian of the Utah Shooting Sports Council said he liked the bill "in concept," but said he had concerns with the bill. He wanted to ensure that no one was surrendering someone else's firearm. Pitcher said his bill would not allow police to accept someone else's gun.
Aposhian said he also had concerns about a provision in the bill that allowed police to keep the firearm if it went unclaimed after a certain period of time.
"Let's see if we can get something in the statute that yes, protects someone who may be temporarily despondent and shouldn't have access to a firearm, but protects their rights as well," he said.
HB121 is expected to have a hearing in the coming weeks
Me too.
I'd just like to see if there are hoops one has to jump through to get the firearm back like proof the emergency is difused or will they just be able to come back and claim their property.
Quoting annas_momma:
I see absolutely nothing wrong with a bill like this, and I'm one of those "crazy gun rights people".


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Born in Salt Lake City on Oct. 13, 1921, Elder Hanks was a son of Stanley Alonzo and Maude Frame Hanks. His father was a prominent municipal judge who died when Elder Hanks was 2. His widowed mother reared six of the seven children to maturity. Elder Hanks was the youngest.
Elder Hanks returned from World War II to earn a law degree at the University of Utah. He and his wife, the former Maxine Christensen, are the parents of five children.
An author and compelling speaker, he also wrote the lyrics to one of the church's hymns, "That Easter Morn," was honored with the Silver Buffalo Award by the Boy Scouts of America and served as president of the Salt Lake Temple from 1982-85.
Asked in 1993 by Dennis Lythgoe of the Deseret News what he thought his epitaph could read, Elder Hanks was hesitant to answer but offered a few possibilities:
"A teacher affects eternity. (It's definitely the most fun I've ever had.)"
"We live on in the lives we have influenced for good."
"Through Christ he early caught a glimpse of what man might be. His generous investment as a teacher produced rich dividends in the lives of others."
"I know that thou canst do everything, and that no thought can be withholden from thee ... but now mine eye seeth thee. Wherefore I abhor myself, and repent in dust and ashes." — Job 42:1-6.
"I would have ended my last general conference address with Job but didn't have the time. I think these verses mean that Job sees that what he did pales beside that of the Savior. If I had anything on my epitaph, I would be happy with these verses from Job."
Funeral services are being planned for Aug. 13.
After receiving emeritus status, Elder Hanks became chairman of the Ouelessebougou Mali-Utah alliance group, which has supported a program of community service for a consortium of villages in Mali, West Africa.
In addition, he chaired the International Enterprise Development Foundation, which assists people in the Philippines and Third World countries in establishing small-business and other economic improvement efforts.
In April 1993, he received an honorary doctorate of Christian service as the main speaker at BYU's graduation.
Elder Hanks had also continued as a public speaker in his later years. For example, in 2002, he gave a talk titled, "I Do Not Do My Work in the Spirit of Benefaction but of Atonement" (a quotation from Albert Schweitzer), at Utah Valley State College in Orem.
He received BYU's David M. Kennedy Public Service Award in 1995. When he received that award, Ray Hillam, Kennedy Center associate and emeritus BYU faculty member. said, "The career of Marion D. Hanks has been a career of service. We cannot recognize all of his accomplishments. They are legion. However, the center wishes to honor Marion D. Hanks for his service in two specific areas: refugee work and rural and free enterprise development."
LDS-oriented Southern Virginia University in Buena Vista has also honored Elder Hanks with its Leader-Servant Award.
Elder Hanks was executive director of the Priesthood Department at the time he received emeritus status. He had also been executive director of the Correlation Department and chairman of the Communications Coding Committee.
As a youth, he won the Utah State Marble Championship, attended West High School and was offered a basketball scholarship to the University of Utah, but declined to serve a church mission.
His mission to the Northern States was cut short by World War II. He served in the Navy where he was group leader of 600 LDS servicemen.
On another assignment, while on an extended tour through the South Pacific, he was the only LDS member aboard a submarine chaser. Appointed acting chaplain by the ship's captain, he conducted weekly services, attracting many of the crew. He achieved the rank of first class petty officer.
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Kids' Sports & Activities
I'm taking my time this morning. I've got to start pet sitting (again..lol) until Sunday when I come home for 1 night and then go right back for 1 night. lol I also have my houses to clean and go to the gym. lol
I might go look around at the mall or something later on this afternoon. Not sure yet. :)
Uggg...we did. That when the las one came out...it was a long ass day! There were about 5 friends that went.
I'm not doing the marathon this time, but we are going to the midnight premier.
Quoting louannwilkins:
Woo Hoo!! Sounds awesome!! Are you an Edward fan or Jacob? :)
Quoting Bob192:
Going to see a Twilight Marathon tomorrow! Pretty excited, almost a whole day to myself!


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Jefferson Robbins of Film Freak Central contemplates Mad Men and concludes, "The only thing left for me to discuss is the hidden star of the show, something designed to go unnoticed unless you squint." The result is this compelling and illuminating exploration of the cinematography.
Posted by Vanderleun at August 13, 2009 1:24 PMFascinating!
Posted by: Obi's Sister at August 13, 2009 3:29 PMit is good to know some people reject the shaky-cam.
Posted by: sTevo at August 13, 2009 6:37 PMThis video confirms that you can learn more by ignoring the minute-by-minute news/info channels and randomly choosing topics and gaining depth in them. Eventually what you learn from an expert in a field, any field really, will be key in understanding some current event or topic. Using this wide and deep method, rather than the frequent and shallow method of mass media, you are less prone to media hysterias of the moment. Three cheers for the internet and podcasts.
Viva Gerard for bringing us such gems.
Posted by: Scott M at August 14, 2009 3:52 AMThat was really great. Thanks for passing this along.
Posted by: Mumblix Grumph at August 14, 2009 4:55 AMyour paintings are sick and racist...JERk!!
Posted by: priscilla nelson at October 14, 2011 12:46 PM


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Crusader: No Remorse Cheats :
Cheats - Ammunition refill
by Unregistered Apr 30, 2005
While playing a game, type "jassica16". If you entered the code correctly, a voice will speak. Then, enter one of the following codes to activate the corresponding cheat function: |
Cheats - Ammunition refill
by Unregistered Apr 26, 2005
Type jassica16 during game play. A voice will |
Crusader: No Remorse Cheats: |


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Google Nexus 7 Tablet Available at SportsFanPlayground.com Starting at $249.99
The Sports Fan Playground (SportsFanPlayground.com) announces that the store has the new Google Nexus tablet available in the 8GB and 16GB versions from $249.99 for the 8GB and $305.99 for the 16GB version, while supplies last.
Detroit, Michigan (PRWEB) April 27, 2013
The Sports Fan Playground announces that the Google Nexus 7 tablet, the popular new device developed by Asus, is available at the Sports Fan Playground online store in 8GB and 16GB models, starting at $249.99.
The tablet features a NVIDIA quad-core Tegra 3 processor, allowing for strong operational power, a 7-inch display, and up to 10 hours of battery life. The store also carries various accessories to complement the features and the style of the Nexus.
The company also announced on Saturday that the newest EA game NCAA Football 13, is available for $39.98 for the Xbox 360 and $37.30 for the Playstation 3. (while supplies last.) The game is the college football game that comes equipped with the Heisman Trophy mode, enhancement from ESPN, and an extensive dynasty mode.
The store also announced that preorders are now being taken on the new NBA 2K13 video game for the Xbox 360 and Playstation 3. The store will also have a special NBA 2K13 Dynasty Edition available for both the 360 and PS3 which will include the video game, a custom NBA 2K13 Spalding basketball, 2K Sports earbuds, and the 2K13 basketball-textured controller skin from Skinit.
For more information, visit SportsFanPlayground.com
On Social Media –
Facebook – Facebook.com/sportsfanplayground
Twitter – Twitter.com/sportsfanplay
Pinterest – Pinterest.com/fanplayground
Google Plus – plus.google.com/115104847008351751780
About SportsFanPlayground.com -
The Sports Fan Playground specializes in apparel and memorabilia from the NFL, NBA, NHL, MLB, International Soccer, NCAA and many more. The store is a leading online e-commerce provider of electronics which range from tablets, PCs and cameras to video games, DVDs, Blu-Ray Discs, appliances, lawn and garden products and more.
The store also carries the top brands in electronics and apparel including Nike, Adidas, Apple, Sony, Samsung, Microsoft, EA Sports, and many more. The company recently began its initial expansion into clothing and jewelry and will continue to expand over the next several months.
For the original version on PRWeb visit: http://www.prweb.com/releases/prwebSportsFanPlayground/GoogleNexus7/prweb9723348.htm