text:
Sharenator.com Pics Cars :))) Fiat Panda 100hp 5BMPH065D 400
It's your #0 visit here. It's about time to stop lurking!
Sharenator.com is all about sharing, voting, competing and simply having fun. Who's the best at posting the coolest stuff? Join now and benefit from all of sharenator's features available only to members. Be the Sharenator!


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In mid-March, the first 13 episodes of "Iron Brides" will be carried on cable TV from NH's Lakes Region, south to Cape Cod. Part reality TV, part game show and part primer for our brides-to-be, "Iron Brides" is fresh, new and a whole lot of fun.
The brides will be competing for a dream wedding photography and videography package worth $9,000 and other prizes as offered by the show's sponsors. Contestants were pampered with massages, worked with life coaches and personal trainers and were tested physically to better prepare and test them for the competition.
SNHU student Amy Dole and alumna Nicole Corvini are Season 1 contestants. They took a few minutes to discuss why they tried out for "Iron Brides."

subset: commoncrawl


text:
Welcome to Redwood Endodontics. We look forward to working with you and your dentist in providing you with the best care for your endodontic needs. Our staff is friendly and works hard to make your visit enjoyable and efficient. Our office is located in the center of the Salt Lake valley and is a modern, state of the art facility utilizing all of the latest technology. We combine this technology with our training and years of experience to ensure that you will receive the best care possible.
6287 Redwood Road | Suite 203 | Taylorsville, UT 84123 PH: 801.266.7393 FX: 801.266.0212
© Copyright 2006 Redwood Endodontics. All Rights Reserved


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The GSX was offered as a high performance package of the GS. |
1970 Buick GS 455
Many Buick performance fans consider 1970 as the greatest year for Buicks 455 engine as it was the most powerful engine Buick built. General Motors lifted it's corporate ban of engines larger than 400 cubic inches in an intermediate body, and Buick quickly responded by adding the brand new 455 cubic inch engine into its restyled GS. The 455 boosted more displacement, bigger valves, and a hotter cam and included standard cold air induction through functional hood scoops. The 455's was rated at 350hp and a pavement pounding 510lb-ft of torque. The Stage 1 package, was even more with a hotter cam, bigger valves, and a revised carburetor. Buick rated it at 360hp, but most testers believed it was more than 400hp.
1970 Buick GSX 455 Stage I
A rare and scarce Muscle Car that is definitely a top dog in the Muscle Car Market. The GSX Stage 1 is a hot car with a Rear Spoiler, Unique Stripes, Hurst Shifter, Heavy-Duty Suspension, Tach, Front Disc Brakes and a functional hood with scoops. Only 678 1970 GSX's were built with two color options. 178 GSX's were built in Apollo White and 491 were built in Saturn Yellow. Original Purchase price was about $4478.00.
1970 Buick GS 455 Production
Model Number | Body/Style Number | Body Type & Seating | Factory Price | Shipping Weight | Total Production |
GSX Sub-series of the Gran Sport 455 | |||||
44600 | 37 | 2 dr Hardtop Coupe - 6P | $3,283 | 3,562 lbs | 8,732 |
44600 | 67 | 2 dr Convertible - 6P | $3,469 | 3,619 lbs | 1,416 |
Note: Only 678 total (convertible and hardtop) GS 455's were produced with the GSX option package


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Sets out requirements for the sampling from moving streams and stationary situations, including stopped-belt sampling, to provide gross samples for sample preparation. The procedures recommended may not be applicable in cases of extreme segregation, e.g. very wet ore due to its sticky nature, or very dry ore due to generation of dust.
Edition: 1 (Monolingual) ICS: 73.060.40 Status: Published Stage: 90.60 (2014-03-19) TC/SC: ISO/TC 129 Number of Pages: 26 -
No revision information available
No corrigenda or amendments available


text:
UT Arlington | find people | maps | submit an event
"Magnetic Capsule Robots for Gastrointestinal Endoscopy and Abdominal Surgery" Pietro Valdastri Dept. of Mechanical Engineering, Vanderbilt University Abstract: The talk will move from capsule robots for gastrointestinal endoscopy toward a new generation of surgical robots and devices, having a relevant reduction in invasiveness as the main driver for innovation. Wireless capsule endoscopy has already been extremely helpful for the diagnosis of diseases in the small intestine. Specific wireless capsule endoscopes have been proposed for colon inspection, but have never reached the diagnostic accuracy of standard colonoscopy.
Office of University Communications(for inquiries on individual events, please contact the event sponsor)


text:
I am sure this has already been discussed, but I could not find a thread on it. Does anyone know how big the rear ballast tank is on the '03 Mobius LS?
I was reading the boat edition of Skiing magazine and the Mobius LS they tested had a ballast size of 750lbs. I assuming that meant 400lbs in the rear and 350lbs in the ski locker??? Does anyone know for sure?
Results 1 to 2 of 2
Thread: Size of stock ballast
02-19-2006, 03:19 PM #1Member
- Join Date
- May 2005
- Posts
- 62
Size of stock ballast
Replying to Topic 'Size of stock ballast'
The rear tank on a mob ls is 350 lbs. Front bag is 400 lbs.
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text:
Commercial and Domestic work undertaken, HPS is an established business in Portsmouth we are known for our cleanliness, reliability and skill. At HPS we understand the need for our customers to see that we keep their properties clean and tidy throughout our works and clear them of any debris on completion.
All of our surveys and reports are free of charge.
We have more than 20 years experience within the trade.
We are approved contractors by Antel Ltd and Wall fast ltd. We take great care in our work and supply Guarantees of our own and Insurance backed Guarantees available upon request.
Cellar Tanking/Membrane systems, Damp Proof courses, waterproofing,penetrating Damp,condensation control all undertaken.


text:
Faculty of Science |
HOME: Table of content Selected Protocols |
Different Disruption Methods |
Apoptosis Inhibitors Cell Division/Cell Cycle/Cell Adhesion Inhibitors Lipid Signal Inhibitors Neurobiology/Neurodegeneration Inhibitors Nitric Oxid/Oxidative Stress Inhibitors Phosphorilation/Dephosphorilation |
Endotoxin removal Cell Penetration |
Cyclodextrins Antifoams |
Dialysis / Desalting columns |
Preparation of Cell Lysates / Different Disruption Methods
GE-HEALTHCARE Protein Sample Preparation Handbook (pdf)
Overview of protein sample preparation
Sample collection, stabilization, and protein extraction
Increasing detectability of targeted proteins
Ensuring compatibility in protein sample preparation workflows
MICROFLUIDICS Choosing The Best Cell
Disruption Method for Research and Pharmaceutical Production (pdf)
A comprehensive selection
of sample preparation tools for three main areas of protein
research: expression proteomics, functional
proteomics, and structural
proteomics (pdf)
BugBuster® Protein Extraction Reagents.
Extraction of active protein from E. coli without sonication
BugBuster (primary amine-free)
Protein Extraction Reagent. Is a special formulation
of BugBuster designed for applications where primary amines
would interfere if present in the protein extract, such as protein
immobilization or cross-linking.
ProteoExtract® Complete Bacterial
Proteome Extraction Kit (C-PEK) is designed for fast and
easy extraction of total proteins from bacteria without
the need for sonication or
precipitation. (pdf-II)
ProteoExtract® Partial Bacterial
Proteome Extraction Kit (P-PEK) provides efficient and
standardized extraction of cellular proteins into four distinct
partial proteomes. The most soluble proteins are released by
mechanical disruption in extraction reagent 1, while extraction reagent
2 releases proteins
intermediate solubility. Reagent 3 provides efficient extraction of
membrane proteins.
SDS buffer solubilizes proteins still insoluble in reagent 3. (pdf-II)
YeastBuster™ Protein Extraction
Reagent. Efficient extraction of protein from yeast
and plants without mechanical disruption and enzymatic lysis
NucBuster™ Protein Extraction
Kit provides an alternative to the time-intensive and cumbersome
traditional methods for preparing nuclear
extracts from mammalian cells.
The entire procedure yields ready-to-use nuclear extract
within 30 minutes from start to finish (pdf-II)
CytoBuster™ Protein Extraction
Reagent is a proprietary formulation of detergents optimized
for efficient extraction of soluble proteins from
mammalian and insect cells. (pdf-II)
PhosphoSafe™ Extraction Reagent
efficiently extracts cytosolic proteins from mammalian
and insect cells while preserving their phosphorylation state.
This reagent contains the same
formula as CytoBuster™ Protein Extraction Reagent (1),
but also includes four phosphatase inhibitors: sodium
fluoride, sodium vanadate, b-glycerophosphate, and
sodium pyrophosphate. (pdf-II)
ProteoExtract® Complete Proteome
Extraction Kits (C-PEKs) are designed for fast and easy extraction
of total proteins from yeast and mammalian
cells and tissues, without the
need for sonication or precipitation. (pdf-II)
ProteoExtract® Partial Proteome
Extraction Kits (P-PEKs) provide efficient and standardized
extraction of yeast and mammalian cellular
proteins into four distinct partial proteomes.
The most soluble proteins are released by mechanical disruption in
extraction reagent 1,
while extraction reagent 2 releases proteins of
intermediate solubility. Reagent 3 provides efficient extraction of
membrane proteins. SDS
buffer solubilizes proteins still insoluble in
reagent 3. (pdf-II)
ProteoExtract® Subcellular Proteome
Extraction Kit (S-PEK) is designed for fast and reproducible
extraction of subcellular proteomes from
mammalian tissue and adherent and suspension-grown
cells. The stepwise extraction delivers four distinct protein fractions
from one sample: (pdf-II)
• Cytosolic protein fraction
• Membrane/organelle protein fraction
• Nuclear protein fraction
• Cytoskeletal protein fraction
The ProteoExtract® Native Membrane
Protein Extraction Kit (M-PEK) is designed for the isolation
of native membrane proteins from mammalian
cells and tissue. The membrane
protein fraction is separated in less than two hours
without the need for ultracentrifugation or incubation of
samples at elevated temperatures. (pdf-II)
PopCulture® Reagent is a detergent-based
concentrate that can be added directly to cultures of
E. coli to effectively extract proteins without the
need for cell harvest. The entire culturing,
extraction, and purification process can be performed in the original
culture tube or multiwell plate. (pdf-II)
RoboPop™ Solubility Screening Kit
is designed for protein expression–level and solubility screening
in a 96-well format and includes all reagents
and plates necessary to perform parallel processing
of 96 bacterial cultures. The kit contains PopCulture® Reagent and
Lysonase™ Bioprocessing
Reagent for efficient extraction of recombinant proteins from E. coli
directly from the culture medium without cell harvest, mechanical
or extract clarification.
Ni-NTA His•Bind® and GST•Bind™ Purification Kits
are designed for high-throughput (HT) purification of soluble
His•Tag® and
GST•Tag™ fusion proteins directly from E. coli cultures. (pdf-II)
Insect PopCulture® Reagent is
a detergent-based lysis reagent specifically formulated for
total insect cell culture extraction without the need for
centrifugation. (pdf-II)
RoboPop™ Ni-NTA His•Bind® Purification Kit
Filtration-based, 96-well format purification directly from transfected
cultures of insect
cells (pdf-II)
BugBaster Bacterial
Protein Extraction (pdf)
Lysonase Bioprocessing Reagent
culture Reagent. PopCulture® Reagent is used for extraction of
proteins from liquid cultures of E. coli without harvesting the
Addition of
0.1 culture volume of PopCulture directly to cells in medium, grown at
any scale, efficiently extracts proteins while retaining their
Protein Extraction Reagents Application Guide. Gentle, efficient,
non-mechanical extraction of soluble proteins from bacteria,
mammalian and
insect cells: BugBuster®, YeastBuster™, and
CytoBuster™ Protein Extraction Reagents. (pdf)
PIERCE B-Per Bacterial Protein
Extraction Reagent (pdf)
QIAGEN: Qproteome™ Bacterial Protein Preparation Handbook. For
preparation of soluble proteins from bacterial cell cultures
QIAGEN: Qproteome Mammalian Protein Prep Kit provides gentle
but efficient detergent-based lysis of mammalian cells to
deliver a total protein
suitable for any downstream application. (pdf)
QIAGEN: Qproteome Cell Compartment Handbook. For fast and
easy subcellular fractionation of intact eukaryotic cells. By
sequential addition
of different
extraction buffers to a cell pellet, proteins in the different cellular
compartments can be selectively isolated: cytosolic proteins
membranes and compartmentalized organelles, such as nuclei,
mitochondria, endoplasmic reticulum (ER), and cytoskeletal
proteins. (pdf)
QIAGEN: Qproteome™ Plasma Membrane Protein Handbook. For fractionation
of plasma membrane proteins from adherent cell culture
` samples. The
kit use a ligand specific for molecules on the plasma membrane
vesicles that allow their elution under native conditions
ligand–vesicle complexes are precipitated using magnetic beads
that bind to the ligand. (pdf)
ROCHE Complete Lysis
Kits: Rapid Protein Extraction and Simultaneous Protection
Against Proteases. The
complete Lysis-B (2x), -M, and -Y
Kits contain
an efficient and gentle Lysis Reagent that consists of non-ionic
detergents that enable efficient and gentle lysis of bacterial
and insect
cells, and the complete Protease Inhibitor Cocktail Tablets. The kits
are also available as EDTA-free version. (pdf)
ROCHE Complete
Lysis-B (2×) Reagent set for highly efficient protein extraction from
bacteria and insect cells by rapid lysis and concurrent
of extracted
proteins against a multitude of proteases (pdf)
ROCHE Complete
Lysis-M. Reagent set for highly efficient protein extraction
from mammalian cells by rapid lysis and simultaneous protection
proteins against a multitude of proteases (pdf)
ROCHE Complete
Lysis-Y. Reagent set for highly efficient protein
extraction from yeast cells by rapid lysis
and concurrent protection of extracted
against a multitude of proteases (pdf)
Protein Extraction (mini-scale) Sonication
Protein Screen (DDM lysis) - Small affinity binding
FACILITY of THE HEBREW UNIVERSITY Sonication
of bacterial samples
The Cytosol/Mitochondria Fractionation Kit is designed for extraction
of cellular proteomes from mammalian cells and deliver
two distinct proteins fractions: a mitochondrial
fraction and a cytosolic fraction. The procedure is easy to perform
and does not rely upon
ultracentrifugation. Protein fractions are suitable
for many downstream applications including 1D and 2D gel electrophoresis,
and apoptotic and signal transduction-related
translocation studies. (pdf)
FOCUSTM SubCell kit enables the fast and easy enrichment
of nuclear, mitochondrial, membrane and cytosolic fractions
from animal cells. The mitochondrial fraction
can be subsequently separated into heavy and light fractions by gradient
centrifugation. An additional
step is included to minimize contaminations of
the nuclear fraction by cytoplasmic elements. The majority of mitochondria,
isolated with this
kit, contain intact inner and outer membranes.
The kit is suitable for cultured animal cells and can be adapted for
animal tissues. (pdf-I) (pdf-II)
A comprehensive selection
of sample preparation tools for three main areas of protein
research: expression proteomics, functional
proteomics, and structural
proteomics (pdf)
The NucBuster™ Protein Extraction Kit
provides an alternative to the time-intensive and cumbersome
traditional methods for preparing nuclear
extracts from mammalian cells. The entire procedure
yields ready-to-use nuclear extract within 30 minutes from start to
finish (pdf-II)
ProteoExtract® Subcellular Proteome Extraction Kit (S-PEK) is
designed for fast and reproducible extraction of subcellular proteomes
mammalian tissue and adherent and suspension-grown
cells. The stepwise extraction delivers four distinct protein fractions
from one sample:
• Cytosolic protein fraction
• Membrane/organelle protein fraction
• Nuclear protein fraction
• Cytoskeletal protein
fraction (pdf-II)
The ProteoExtract® Native Membrane
Protein Extraction Kit (M-PEK) is designed for the isolation
of native membrane proteins from mammalian
cells and tissue. The membrane protein fraction is
separated in less than two hours without the need for
ultracentrifugation or incubation of
samples at elevated temperatures.
PIERCE Lysosome Enrichment Kit for Tissue
and Cultured Cells enables isolation and enrichment for intact
lysosomes from crude cell and tissue
extracts. The kit
provides sufficient reagents for preparing 25 extracts and uses
OptiPrep™ Cell Separation Media for the density-based
separation of
lysosomes from contaminating cell structures. The isolated lysosomes
may be
used for a number of downstream applications,
2D/MS for proteomics research, electron microscopy, disease profiling
and gene expression, signal transduction, and interaction
localization. (pdf)
PIERCE Mitochondria Isolation Kit for Tissue (pdf)
Offers two methods for isolation of intact mitochondria from both
soft and hard tissues.
first method uses a unique reagent-based procedure that enables
simultaneous multi-sample processing. The second method relies on
traditional Dounce homogenization for tissue
disruption and subsequent isolation of the organelle. Both procedures
rely on
centrifugation to separate the intact mitochondria using a bench-top
microcentrifuge and are completed in less than 60 minutes.
PIERCE Nuclei Enrichment Kit for Tissue enables isolation of an
enriched sample containing intact nuclei from 400-500 mg
of either hard or soft
tissue. The kit
contains OptiPrep™ Cell Separation Media and optimized buffers
along with detailed instructions for the enrichment of nuclei
with minimal
contamination from golgi bodies and endoplasmic reticulum. The isolated
intact nuclear sample contains all the nuclear proteins,
material and other intact nuclear structures, allowing downstream
applications such as enzymatic assays, electron microscopy, Western
blotting and
2D/MS analysis. (pdf)
Peroxisome Enrichment Kit for Tissue enables
isolation of an enriched peroxisome sample from hard or soft
tissue such as rat liver, kidney
heart tissue. The kit provides sufficient reagents for preparing 25
extracts and uses OptiPrep™ Cell Separation Media for the
separation of
peroxisomes with minimal
contamination from golgi bodies and endoplasmic reticulum. (pdf)
PIERCE Subcellular
Protein Fractionation Kit enables stepwise separation and preparation
of cytoplasmic, membrane, nuclear soluble, chromatin-bound
cytoskeletal protein extracts from mammalian cultured cells or tissue.
The first reagent added to a cell pellet causes selective cell membrane
releasing soluble cytoplasmic contents. The second reagent dissolves
plasma, mitochondria and ER/golgi membranes but does
not solubilize
nuclear membranes. After recovering the intact nuclei by
centrifugation, a third reagent yields the soluble nuclear extract. A
extraction with micrococcal nuclease is performed to release
chromatin-bound nuclear proteins. The recovered insoluble pellet is then
extracted with
the final reagent to isolate cytoskeletal proteins. (pdf)
QIAGEN: Qproteome™ Plasma Membrane Protein Handbook. For fractionation
of plasma membrane proteins from adherent cell culture
samples. The
kit use a ligand specific for molecules on the plasma membrane
vesicles that allow their elution under native conditions
ligand–vesicle complexes are precipitated using magnetic beads
that bind to the ligand. (pdf)
QIAGEN: Qproteome™ Mitochondria Isolation
Handbook. For purification of mitochondria from eukaryotic cells. (pdf)
QIAGEN: Qproteome™
Nuclear Protein Handbook. For isolation of nuclear and nucleic-acid binding
proteins from eukaryotic cell lysates
QIAGEN: Qproteome Cell Compartment Handbook.
For fast and easy subcellular fractionation of intact eukaryotic
cells. By sequential addition
of different
extraction buffers to a cell pellet, proteins in the different cellular
compartments can be selectively isolated: cytosolic proteins
membranes and compartmentalized organelles, such as nuclei,
mitochondria, endoplasmic reticulum (ER), and cytoskeletal
Choice of
Healthcare Recommended buffers for
Anion Exchange Chromatography
Healthcare Recommended
buffers for Cation Exchange Chromatography
Healthcare Recommended Volatile buffers
systems for Ion Exchange Chromatography
AMRESCO Buffers Technical
Bulletin (pdf)
CALBIOCHEM Buffers - A guide for the preparation and
use of buffers in biological systems (pdf-II) (pdf)
Table of detergents and
buffers (pdf)
SIGMA Useful
pH Ranges of Selected Biological Buffers (pdf)
Calbiochem Inhibitor SourceBook (pdf)
Division/Cell Cycle/Cell Adhesion Inhibitors
Calbiochem Inhibitor SourceBook (pdf)
Signal Inhibitors
Calbiochem Inhibitor SourceBook (pdf)
Calbiochem Inhibitor SourceBook (pdf)
Oxid/Oxidative Stress Inhibitors
Calbiochem Inhibitor SourceBook (pdf)
Calbiochem Inhibitor SourceBook (pdf)
PhosphataseArrest™ II is a phosphatase inhibitor cocktail consisting
of 5 phosphatase inhibitors that target acid, alkaline and
phosphatases. (pdf)
Phosphatase Inhibitor Cocktail
Sets (pdf)
PIERCE: Halt™ Protease and Phosphatase
Inhibitor Cocktail (pdf)
Halt™ Phosphatase Inhibitor Cocktail.
Protects phosphoproteins from serine/threonine phosphatases
as well as protein tyrosine
phosphatases (PTPs).
The cocktail contains a mixture of four inhibitors of broad
specificity, including sodium fluoride, sodium orthovanadate,
sodium pyrophosphate and
β-glycerophosphate. (pdf)
PROTEINKINASE A comprehensive source of kinase signaling activators,
substrates, inhibitors and kinase-related products.
ROCHE PhosSToP
Phosphatase Inhbitor Cocktail Tablets to inhibit a broad range of phosphatases. (pdf)
Protease Inhibitors
CALBIOCHEM Calbiochem Inhibitor SourceBook (pdf)
CALBIOCHEM Protease Inhibitor (pdf)
CALBIOCHEM Protease
Inhibitor (pdf)
G-BIOSCIENCES A handbook & selection guide for inhibitors of protease & phosphatases (pdf)
NOVAGEN Protease Inhibitor Cocktail Sets
(with or without EDTA) for bacterial, fungal and
yeast cell extracts (pdf)
PIERCE: Halt™ Protease and
Phosphatase Inhibitor Cocktail (pdf)
Protease Inhibitor Guide (pdf-I)
Universal Protease
Substrate (pdf)
Protease Inhibitors (pdf)
SIGMA Protease
and Phosphatase Inhibitor Cocktails (pdf)
CALBIOCHEM Calbiochem Inhibitor SourceBook (pdf)
DNA removal
Benzonase Nuclease - Removal of Nucleic
Acids (pdf)
: Lysonase
Bioprocessing Reagent (pdf)
Removal of Nucleic Acids - Different
of DNA from lysates of E. coli cells
and French bean tissue prior to electrophoresis.
Endotoxin removal
Endotoxin Clearance
with Triton X-114 – Selected protocols from the literature
BIORAD Affi-Prep®
Polymyxin Matrix - Removal of endotoxin molecules from biological
and aqueous solutions by
chromatography (pdf)
EndoBind-R™ is a DADPA-agaroseconjugated S1 peptide
affinity chromatography column. It has been used to remove
endotoxin from water, buffers, and cell culture
media. Under optimized conditions, it may also be used to produce
protein solutions with high recovery. Additionally,
the S1 peptide is highly resistant to a wide range of pH's and
ionic strengths
making it suitable for many applications in which
traditional chromatography methods, such as ion exchangers, affinity
and immunoaffinity matrices are not applicable (pdf)
CAP CODE Pyrochrome® Chromogenic Endotoxin Testing. Can achieve a maximum sensitivity of 0.001 EU/mL (pdf-I) (pdf-II)
Cellufine® ET clean for endotoxin removal. The
Cellufine ETclean is poly(ε-lysine) immobilized Cellufine®
(cellulose spherical
beads). The beads bind and remove endotoxin from your
solution. The poly(ε-lysine) is a microbial poly(amino acid)
consist of 30-35 lysine residues produced by
Streptomyces albulus. Operating Guidelines: CIP for endotoxin free
Alkali solution and require time for endotoxin free.
(1) To reduce endotoxin from a sample solution
containing acidic protein with pI 4.0-6.5, you can use Cellufine ET
clean-S beads
with a small pore size at pH 5-7 and NaCl concentration of
(2) To
reduce endotoxin from a sample solution containing neutral or basic
protein with pI 7.0-10.5, you can use Cellufine
clean-L beads with a large pore size at pH 7-9 and NaCl concentration
of 0.1-0.4 M
The ToxinEraserTM Endotoxin Removal Resin utilizes immobilized
polymixin B to bind and remove pyrogens from solution.
The polymixins are a family of antibiotics that contain a
cationic cyclopeptide with a fatty acid chain. Polymixin B neutralizes
biological activity of endotoxins by binding to the lipid A
portion of bacterial lipopolysaccharide. (pdf)
GENSCRIPT ToxinSensorTM Chromogenic LAL Endotoxin Assay Kit. A quantitative In Vitro
end-point endotoxin test. The method utilizes
a modified
Limulus Amebocyte Lysate and a synthetic color producing substrate to
detect endotoxin chromogenically. The kit has a
endotoxin detection limit of 0.005 EU/ml and a measurable concentration
range of 0.005 to 1 EU/ml. (pdf)
HYGLOS EndoLISA® new method for efficient and reliable determination of endotoxin (LPS) levels (alternative to LAL). Use a
lipopolysaccharide (LPS) specific phage-derived
protein. Measurement range: 0.05 - 500 EU/ml. pH range: 4 -
interference in high salt conditions e.g. NaCl, GdnHCl (pdf-I) (pdf-II)
HYGLOS Endo
Trap Endotoxin Removal System (pdf-1) (pdf-2)
EndoTrap® blue and EndoTrap® red. EndoTrap is an affinity
matrix for the efficient removal of bacterial endotoxins
from solutions.
(pdf-I) (pdf-II) (pdf-III)
Application protocol for
a FPLC/HPLC automayed system (pdf)
HYGLOS Endotoxin removal methods - advantages & disadvantages (attachment)
MILLIPORE Matrex
Cellufine Sulfate - For concentration, purification
and depyrogenation of Virus, Viral/Microbial Antigen,
binding proteins (pdf)
Endotoxin Removal Kit. Designed for
the rapid removal of endotoxins from purified DNA. (pdf)
(pdf Mini)
Detoxi-Gel Endotoxin Removing gel: immobilized polymixin B
Pierce® High-Capacity Endotoxin Removal Resin. Porous
cellulose beads that have been surface modified with covalently
ε-poly-L-lysine (pdf)
PIERCE: The Thermo Scientific Pierce LAL Chromogenic Endotoxin Quantitation Kit is an
efficient, quantitative endpoint assay for the detection
gram-negative bacterial endotoxins. Bacterial endotoxin catalyzes the
activation of a proenzyme in the modified Limulus Amebocyte Lysate
(LAL). The
activated proenzyme then catalyzes the splitting of p-Nitroaniline (pNA) from the
colorless substrate, Ac-Ile-Glu-Ala-Arg-pNA;
the activation
rate is proportional to the sample endotoxin concentration. After
stopping the reaction, the released pNA is photometrically
measured at
405-410nm. The correlation between absorbance and endotoxin
concentration is linear in the 0.1-1.0EU/mL range. The developed
intensity is proportional to the amount of endotoxin present in the
sample and can be calculated using a standard curve. (pdf)
Pall Protein Sample
Preparation and Analysis Application Manual: 2.6 Endotoxin Removal
VIVASCIENCE Removal
of endotoxin from monoclonal antibodies
using Vivapure™ centrifugal ion exchange
membrane devices (pdf)
Y. Aida and M. Pabst Removal of endotoxin
from protein solutions by phase separation using Triton X-114
Journal of lmmunological Methods,
(1990) 191-195 (pdf)
Anspach, Friedrich Birger Review: Endotoxin removal by affinity sorbents J. Biochem. Biophys. Methods 49 (2001) 665–681 (pdf)
T. Zimmerman et al. Simultaneous metal chelate affinity purification and endotoxin clearance of recombinant antibody fragments
Journal of Immunological Methods 314 (2006)
67–73 (pdf)
Z. Chen et al. Efficient
production of recombinant IL-21 proteins for
pre-clinical studies by a two-step dilution refolding method
Immunopharmacol (2013), http://dx.doi.org/10.1016/j.intimp.2013.02.017
How to purify a membrane protein-Technical bulletin
ANATRACE Structure of Detergents (pdf)
ANATRACE Detergent Properties List (pdf)
ANATRACE Detergents and their
use in membrane protein science. Handbook that introduce the researcher
to the physical and chemical
properties of detergents and describe how these
properties relate to detergent function. (pdf) (pdf-II)
ANATRACE Amphipol A8-35 Amphipathic surfactant for maintaining solubilized membrane proteins in
detergent-free solutions. Serve as
stabilizers of membrane proteins in aqueous
solutions. Amphipols can substitute out the detergents used to extract
membrane proteins,
keeping them
soluble in detergent-free aqueous solution while stabilizing them
Applications: Stabilization of native membrane protein structure. Facilitating membrane protein folding /refolding. NMR, etc (pdf)
ANATRACE Neopentyl
Glycol (NG) class detergents are revolutionary new amphiphiles which
have already shown great utility in membrane
protein studies. NG class detergents are a more
effective detergent for extracting and solubilizing/ stabilizing
proteins, and are particularly
beneficial in crystallization due to his
revolutionary new architecture that allows the molecule to pack densely
when forming a micelle,
producing exceptionally low critical micelle
concentrations and extreme water solubility (pdf)
Nature Methods: "Maltose–neopentyl glycol (MNG) amphiphiles for solubilization,
stabilization and crystallization of membrane proteins"
by groups from
Jean-Luc Popot, Brian Kobilka, and Samuel H. Gellman labs (pdf)
ANATRACE Application References (pdf)
CALBIOCHEM Biological Detergents. Guide for
solubilization of membrane proteins and selecting tools for
detergent removal (pdf)
CALBIOCHEM A guide to the properties
and uses of detergents in Biology and Biochemistry
CALBIOCHEM Table
of detergents and buffers (pdf-I) (pdf-II)
G-BIOSCIENCES A Handbook & Selection Guide to Detergents & Detergent Removal . Dye solubilization assay for CMC determination (pdf)
G-BIOSCIENCES Optimizer blueBALLS™. A simple product and method to determine the optimal detergent concentration. Glass balls
that have been
coated with a hydrophobic blue dye that has the same properties as
membrane proteins. The solubilization of the dye
only occurs
when micelles have been formed. (pdf)
SDS removing buffer designed for removing
bound-SDS from protein sample using ProteoConD kit.
Healthcare Membrane
Protein Purification Kit. Efficient detergent screening of
histidine-tagged membrane proteins. Small aliquots of cell
membranes are solubilized in different detergents
followed by rapid purification using His Mag Sepharose™ Ni. The
purification step
is performed directly after
solubilization using the same detergent. Analysis and evaluation can be
performed by a number of methods
such as Western blot, gel
filtration, or light scattering. (pdf)
JENA BIOSCIENCE JB Screen Detergents cover all detergents
that have been successfully used for the crystallization
of membrane proteins.
(pdf-1) (pdf-2) (pdf-3)
Detergent Removal with Amicon®
Microcon® and Centricon® Centrifugal Devices (pdf)
Removal of different detergents after one spin
ProteoSpin™ Detergent Clean-Up Kit. Provides
a fast and simple procedure for the removal of SDS, Triton® X-100
and other detergents
from acidic or
basic protein samples. It is designed to remove all types of detergents
protein solutions either in their free form or bound form,
as when
complexed with
the protein. Purification is based on spin column chromatography
using Norgen's proprietary resin as the separation
matrix. (pdf Micro) (pdf Maxi)
PALL SDR HyperD®
Detergent Removal and Solvent removal (e.g. Tri-n-Butyl Phosphate
– or TnBP). Stable in acid, polar organic
and oxidizing
solutions. (pdf-I) (pdf-II)
PALL Protein Sample Preparation
and Analysis Application Manual:
2.3 Detergent Removal (pdf2)
PIERCE: Extracti-Gel D Detergent Removing gel (pdf)
PIERCE: Remove detergent from protein samples (pdf)
of Removal Methods. General Detergent Properties. Appropriate
Methods of Removal for Specific Detergents
Detergents. Detergent Properties and Applications. Detergent Types and Selection.
Detergent Selection Table. BioUltra Detergents
(pdf - see pg 13)
Cyclodextrins (pdf
- see pg 32)
Antifoams (pdf
- see pg 33)
Detergent Removal (pdf - see pg 35
SIGMA Detergents Properties and Applications (pdf)
Table of detergents (pdf)
Mohanty A. et.al. (2003) Inhibition of tobacco etch virus protease activity by detergents. Protein Expression and Purification 27: 109–114 (pdf)
Cell Penetration
ABBIOTEC Lipodin-ProTM is a
protein transfection reagent, for transporting biologically active peptides
and proteins directly into living cells.
Is a lipid-based formulation that
is added to the protein of interest minutes before cell delivery. Within few
hours, protein activity can be observed
in living or fixed cells. Ideal for monitoring
kinetics of biological
activities in established cell lines and primary cells, including
neurons. No chemical
ligation or crosslinking. Serum
compatible, no cytotoxicity and biodegradable. Up to 95% efficiency in less than 5 hours. (pdf)
ABBIOTEC Lipodin-AbTM is a reagent optimized for Antibody delivery into living cells. A lipid-based formulation
that is added to the antibody
solution minutes before cell delivery. Within few hours, antibody staining can be
observed in living or fixed cells. Ideal for staining
intracellular proteins
without fixation in established cell lines and
cells, including neurons. No need for cell fixation protocol.
Serum compatible, no cytotoxicity and
biodegradable. Deliver functionally active antibody
within hours. Up to
95% efficiency. (pdf)
ACTIVE MOTIVE Chariot™ is a revolutionary delivery reagent that quickly and efficiently
transports biologically active proteins, peptides and antibodies
directly into
cells. The typical delivery efficiency is 60-95%. Less than two hours after
delivery, live cells can be assayed to determine the effects of the
material, without the need for fixing. This makes Chariot an ideal tool for
functional studies, including delivering inhibitory proteins, labeling
organelles, screening peptide libraries and studying
protein half-lives & transient complementation. (pdf)
JENA BIOSCIENCE "Protein
Transduction Domains" (PTDs) or "Cell penetrating
peptides" (CPPs) used to internalize peptides, proteins,
oligonucleotides, nucleic acids,
modified nucleic acids like PNAs or morpholino oligonucleotides and
plasmids. CPPs were successfully
tested on a large number of
different cells including mammalian cells and plant cells, cell
cultures and primary cells; intracellular
delivery into nerve
cells and for penetration of the blood-brain barrier. (pdf-I) (pdf-II)
JBS-Proteoducin: Cocktail developed for internalization of
peptides and proteins into living cells
JBS-Nucleoducin: Internalization of mono- and olgonucleotides into live
The amphiphilic CPPs
MPG , MPG and CAD-2 are designed for formation of non-covalent
complexes with peptides, proteins,
plasmids and nucleic acids and their mimics.
They strongly differs in their polarities.
Penetratin and
HIV-Tat are classical CPPs that are widely used for forming conjugates
or fusion proteins
Concentration / Ultrafiltration / Dialysis / Desalting Columns
concentration by ultrafiltration and buffer solubility screen
AmiKa Corp Produce
a unique range of products for microdialysis of small (10 to 5000 microlitres)
sample volumes (proteins, peptides, nucleic acids
and other
biomolecules), electroelution, electrodialysis,
electro-concentration, Coomassie Blue removal, and
quantitative protein determination
BioToolomics: SuperSpinTM Desaltor (pdf)
Healthcare Selecting. Hollow
fiber. Cartriges and Systems (pdf)
Healthcare Membrane separations:
range of options (pdf)
Healthcare PD-10 Desalting Columns,
PD MidiTrap G-25, PD MiniTrap G-25, PD SpinTrap G-25, and PD MultiTrap
single-use columns and
96-well filter plates for a wide range of applications including
desalting, buffer exchange, and the
removal of
low-molecular weight compounds. Centrifugation protocol with all
gravity columns. (pdf)
Healthcare PD 10 Desalting columns
Healthcare PD SpinTrap G-25 (pdf)
Healthcare Vivaspin 500, 2, 6 and 20. Vivaspin Concentrators
are disposable ultrafiltration devices for the concentration of biological
samples (pdf)
G-BIOSCIENCES The Column PROTEIN-
Concentrate Kit has been specifically developed for concentration
of those proteins that cannot be
either by precipitation or other techniques. The kit is based on a
proprietary Protein Binding Resin that binds and immobilizes
any protein in
low salt buffer between pH 2-12 (capacity ~ 0.5mg protein/ml Protein
Binding Resin). The immobilized protein is spin-eluted
in a small
volume of specifically formulated elution buffer, giving several fold
effective concentration. This kit is suitable for concentration of
a total of 4mg
protein in either single or multiple procedures. (pdf)
G-BIOSCIENCES The OrgoSol PROTEIN Concentration™ kit is based
on precipitation of protein in a proprietary combination of organic
solvents. The kit has been specifically developed
for efficient precipitation of protein solution with a
minimal disruption to the protein
structure and thus maintaining biological activity
of most proteins. This kit is therefore suitable for concentration of
protein solutions
where maintaining biological activity is essential.
G-BIOSCIENCES Tube-O-DIALYZER™ (pdf)
GEBA Maxi
GeBAflex-tube Gel Extraction and Dialysis Kit (3 ml) Handbook.
Applications: Extraction of proteins, RNA, DNA or
from polyacrylamide, agrose
or any gel matrix in any running buffer; dialysis or buffer exchange of
volumes between 0.1-3 ml;
and preparation of protein samples for
MALDI-MS. (pdf-I)
MERCK D-Tube™ Dialyzers D-Tube™ Mini, Midi, Maxi and Mega (pdf pg 29-30)
Amicon® Ultra Centrifugal
Filters (pdf pg 31-42)
Protein Purification, Concentration and Dialysis (pdf) (pdf-II)
MILLIPORE Stirred Ultrafiltration Cells Models
8003, 8010, 8050, 8200, 8400. User Guide. Millipore stirred ultrafiltration
cells are designed for
concentration or purification of macromolecular solutions in volumes
from 3 to 400 mL (pdf)
MILLIPORE Solvent Resistant Stirred Cells
for Ultrafiltration and Filtration Applications
MILLIPORE Model 2000 High-performance Ultrafiltration
Cell. The 2000 series of internally stirred ultrafiltration
cells are designed for rapid
concentration or purification of macromolecular solutions
in volumes up to 2000 ml. (pdf)
MILLIPORE - AMICON ( Ultrafiltration)
Centrifuge Filter Devices (pdf)
Detergent Removal with Amicon® Microcon® and Centricon®
Centrifugal Devices (pdf)
Percentage Removal of different detergents after one
Membrane-based Tangential Flow Filtration (TFF). Membrane-based Tangential
Flow Filtration (TFF) unit operations are used
for clarifying, concentrating,
and purifying proteins. This technical brief is a practical
to protein processing using tangential
flow filtration. It is intended
to help scientists and engineers achieve their protein processing
objectives by discussing how the choice
of key components and operating
parameters will affect process performance. (pdf)
MILLIPORE Ultrafiltration
Membranes for Macromolecule Processing . This guide provides an
introduction to Millipore's family of Ultrafiltration
Membranes and an overview of the
key criteria and product characteristics to consider when selecting an
UF membrane (pdf)
NORGEN ProteoSpin™
CBED (Concentration, Buffer Exchange and Desalting) Kit. The
simultaneous removal of salts while concentrating a dilute
protein solution makes the kit a convenient method
for preparing proteins before running many downstream applications such
as SDS-PAGE
and isoelectric focusing. Purification is based on
spin column chromatography using Norgen's proprietary resin as
the separation matrix. These
large columns are frequently used to prepare protein
samples for structural analysis where larger amounts of protein are
needed, such as
X-ray crystallography, NMR spectroscopy and other
applications. The ProteoSpin™ CBED Maxi Kit comes with solutions
for concentrating
and desalting both acidic and basic proteins. (pdf for Micro)
for Maxi)
NOVAGEN D-Tube™ Dialyzers and D-Tube Electroelution Kit . Easy-to-handle dialyzers in a capped centrifuge
tube format with dialysis membrane
windows for buffer exchange and removal of solutes.
The disposable, single-use tubes require no syringes, centrifuge, or
laborious steps
to manipulate small sample
volumes. Available with molecular weight cut-offs from 3.5 to 14
kDa and are designed with three volume
capacities: mini (10–250 ml), midi
(50–800 ml), and maxi (100–3000 ml). The membrane is
ultra-clean, EDTA-treated regenerated
cellulose, sulfur- and heavy metal-free. (pdf)
NOVAGEN D-Tube96
Dialyzer. The membrane is ultra clean,
EDTA-treated regenerated cellulose, sulfur- and heavy metal-free
and available in
molecular weight cutoffs of 6-8 kDa or 12-14 kDa.
The D-Tube Dialyzers Mini have a capacity of 10-250 μl and are ideal
for small
samples. (pdf)
Pall Protein Sample Preparation and
Analysis Application Manual:
2.4 Concentration, Desalting, and Buffer Exchange (pdf2)
4.4 Buffer Exchange, Desalting, and Concentration
6.2 Optional
Pre-Treatment to Improve Recovery of Samples
from Ultrafiltration Spin Filters (pdf6)
6.4 General Filtration Procedures for AcroPrep™ and AcroWell™
Filter Plates (pdf6)
Pall Centrifugal Devices for Ultrafiltration & Microfiltration. A full range of precise, high-performance products
to rapidly process volumes from
50 µL to
60 mL.
Trisacryl® GF05 M, LS Size Exclusion Chromatography Sorbents. Designed
for desalting and for the separation of small molecules.
40-80 μm
for M grade & 80-160 μm for LS grade for fractionation at high
flow with low pressure, designed for industrial scale. (pdf)
Pall Products
for Sterile Filtration, Clarification and Separation in the Laboratory.
Technologies for Complex Lab Bioprocessing (pdf)
PIERCE iCON™ Concentrator 7 ml/9K,
7 ml/20K, 20mlml/9K and 20mlml/20K
. Disposable ultrafiltration centrifugal devices for concentration
and diafiltration/buffer exchange of biological
samples such as enzymes, antibodies or DNA. (pdf-I)
PIERCE Protein desalting Spin Columns
PIERCE Zeba™ 96-well Desalt Spin Plates contain a resin
that offers high-performance desalting and protein recovery characteristics
sample processing. Sample volumes from 20 to 100 μl can be processed
with >
95% retention of salts and other small
molecules (< 1,000 Da) and exceptional protein recovery. (pdf)
PIERCE Zeba™ Micro Desalt Spin Columns (pdf)
High-Performance Dialysis and Desalting Brochure: Slide-A-Lyzer® MINI Dialysis
Units; iCON™ Protein Concentrators;
Dialysis Tubing and
Desalting Columns (pdf-I) (pdf-II)
PIERCE Dyalisis Cassettes (pdf)
PIERCE MINI Dyalisis Unit (pdf)
Centrifugal Units - Centrisart Units. Spiral Flow
Filtration Cells (10-100ml)
Float-A-Lyzer® • DispoDialyzer® • Micro DispoDialyzer®
Dialysis Membranes with
Different Molecular
Cut-offs (pdf) (pdf-II)
Tangential flow membrane for Biomedical Separation
THE NEST GROUP Ultra-filtration, Dialysis, Spin
Columns or 96 Well Plates for Salt Removal - Buffer Exchange
Salt Removal - Buffer Exchange MicroDialysis,
Equilibrium Dialysis, and Electroelution. Single-Sided Biodialyzer,
Biodialyzer, Disposable
Dialyzers, Equilibrium Dialysis and Membranes and Electroelution (pdf)
Equilibrium dialysis (pdf)
DispoDIALYZER™- Samples from 25μl to 300μl. Range of
membrane MWCO's available: 1K, 2K, 5K, 10K,
and 25K Daltons (pdf)
Micro DispoDialyzer™ - Samples from
5μl to 100μl and Macro Fast DispoDIALYZER™ - Samples from
1ml to 10ml
(pdf )
VIVASCIENCE Ultrafiltration Products Catalogue
(pdf-I) (pdf-II)
Vivapure® C18 Micro spin columns.
Innovative membrane-based devices for concentration, purification
and desalting of peptides
to analysis by mass spectrometry. (pdf-I) (pdf-II) (pdf-III)
of Peptides with Vivaspin 500 Ultrafiltration
Devices (pdf)
HOME: Table of content Selected Protocols |
Dr. Mario Lebendiker
The Protein Purification Facility
The Wolfson Centre for Applied
Structural Biology, The
Hebrew University of Jerusalem
Tel: 972-2-6586920
Copyright ©, 1997, The Hebrew University of Jerusalem. All Rights Reserved |


text:
National Agricultural Library Thesaurus
The NAL Thesaurus is an online vocabulary tool of agricultural terms in English and Spanish and is cooperatively produced by the National Agricultural Library, USDA and the... -
Open Library
About One web page for every book ever published. It's a lofty, but achievable, goal. To build it, we need hundreds of millions of book records, a brand new database... -
Library of Congress Subject Headings
LCSH has been actively maintained since 1898 to catalog materials held at the Library of Congress. By virtue of cooperative cataloging other libraries around the United States...


text:
A reality check from Standard & Poor's
– Neil Collins is a Reuters columnist. The views expressed are his own –
Standard & Poor's could have chosen a better day to kick the British economy, by placing the UK onto "negative outlook", the usual precursor to a downgrade of S&P's rating of an issuer's debt.
The move came minutes before the Debt Management Office closed its massive auction of 5 billion pounds of 2014 stock, and minutes after the release of figures showing the Public Sector Net Borrowing Requirement leaping to 8.5 billion pounds in April, a sum which not long ago would have been considered high for a whole year.
Economist Howard Archer at Global Insight immediately called the figure "dire, starting the new fiscal year off as it is highly likely to continue."
S&P, meanwhile, now fears that the net general government debt burden "could approach 100 percent of GDP and remain near that level in the medium term."
It's hard to describe the UK public finances as anything other than a disaster area. The forecasts made in last month's Budget looked optimistic within days, and even these require the DMO to borrow 220 billion pounds this financial year, or almost a billion pounds every working day.
Yet while the DMO soaks up cash, the Bank of England is desperately creating it. Its "quantitative easing" programme has been in full swing this week, buying in 1.326 billion pounds of a stock which looks very like the one that the DMO was issuing just one day later.
The experts will tell you that because the life of the new stock is not quite five years, it falls outside the Bank's five to 25-year target zone for QE, and is therefore qualitatively different. This is pure mumbo-jumbo. Essentially what is happening is one arm of the government is creating money for another arm of the government to borrow.
The traders can hardly believe their luck, selling expensively to the Bank and buying cheaply from the DMO.
This waste of taxpayers' money would be bad enough, but the real damage is the false sense of security this round-tripping produces. Britain is in real danger of falling into a debt trap, where the cost of borrowing spirals up with the amount the government has to raise.
As the rating agency's reality check concludes: "A government debt burden [of nearly 100 percent of GDP] if sustained, would in S&P's view be incompatible with an AAA rating."
Loss of that rating would lead to a higher cost of government borrowing, damaging the chances of avoiding the trap.
Were the Labour administration not in total funk, it might seize on this report to admit that its spending plans are not sustainable. Capital projects, like the NHS IT scheme, ID cards, Crossrail, aircraft carriers, the Eurofighter and much else will have to go, and the next government will have to impose real cuts in the core spending of education, health and welfare.
S&P's warning shot shows that the phony war is over, and the real pain lies ahead.


text:
Values Learned in the 90s: Bravery (Part 1 of 4)
Through life, we experience many things that shape us to become the people we are today. Preparation to become the adults we are starts in our youth. The 1990s was a pivotal point in my life, and experiencing those years in my youth set me up to become the person I am today.
Many of life's curveballs force us to be braver than we think we are, and however you choose to look at it, being a kid in the 90's definitely helped a little.
The game of "Perfection", a.k.a. a ticking time bomb waiting to send you into cardiac arrest, definitely tested this value firsthand. The bright yellow pieces were to be placed in the proper pegs as time wasted away. If the game exploded before you were done, not only did you lose, but your parents would run downstairs asking what happened because of how loud you screamed.
You had to be a tough cookie in order to finish the puzzle. When you finished it before the game exploded, it didn't even seem that scary when it popped! Bravery set into your little kid veins and you could conquer anything.
You also had to be brave if you were the owner of a Furby in the 90's. I owned one of these little fuzzballs of terror, mostly because I was naïve and didn't know that even if you take the batteries out and shove it in the closet, it would still urge you to "come play" at 2:00 in the morning. True story.
Their re-emergence in recent years has been frightening. The Y2K kids clearly don't know of the misery they caused. Now with electronic eyes, it's more than clear to me that the Furby's comeback is because they are CIA agents receiving information through the kids of today.
I am clearly strong enough now, brave enough now, and ready to tell you, Readers, that Furby scarred me for life through his shenanigans in my childhood.
Thank you, Furby and "Perfection," for creating strong and brave adults through your terrifying reign in the 1990s. There is no doubt that now, through any life experiences coupled with these two things, we are all braver than a little toaster on a vacuum cleaner.
J'na Jefferson
Latest posts by J'na Jefferson (see all)
- Feature: An Open Letter to Winter - March 5, 2014
- Values Learned in the 90s: Acceptance of Change (Part 4 of 4) - February 20, 2014
- Values Learned In The 90′s: Leadership (Part 3 of 4) - February 6, 2014


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Cowichan Valley Caps' captain Mikael Jung tries to control the puck on the doorstep of the Vernon Vipers' net guarded by Austin Smith in BCHL play Saturday night in Duncan.
Vernon Vipers fall 1-0 to Grizzlies
Published: January 21, 2013 11:00 AMUpdated: January 21, 2013 11:43 AM
Myles Fitzgerald fired his 22nd goal of the year midway through the third period as the Victoria Grizzlies clipped the Vernon Vipers 1-0 in B.C. Hockey League action Sunday afternoon before 1,100 fans at Bear Mountain Arena.
The Vipers, who toppled the Cowichan Valley Capitals 3-1 Saturday night in Duncan, were outshot 38-23 by the Grizz, first overall in the league at 28-9-0-4. The Vipers dipped to 14-20-0-7, six points back of the fourth and final playoff spot in the Interior.
Michael Stiliadis (15-9) earned the win, while Austin Smith (10-21) took the loss. Vernon had three powerplay chances and Victoria had six.
On Saturday night, in front of 725 fans at the Island Savings Centre, Colton Sparrow (5th), Dexter Dancs (5th) and Mason Blacklock (11th) supplied the offence as the Vipers stuffed the league's last-place team.
Jamie Waddington replied for the Caps (9-25-1-3). Danny Todosychuk (4-5) registered 31 saves for the win, while Connor LaCouvee (3-7-1) blocked 28 saves in the loss. Both teams unleashed 31 shots.
Meanwhile. Vernon's Alex Gillies clicked at 2:24 of overtime as the fourth-place Salmon Arm SilverBacks edged the Trail Smoke Eaters 4-3 Saturday night before 1,000 fans at Cominco Arena. Gillies, who has 14 goals, tied the game late in the third period to force OT.
Please see Wednesday's sports section for full details.


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High School Insider
With all state championships having been run, this is the highest ranking ever for a Cincinnati boys team, Russo said. The Colerain girls finished third in 1999.
The final Harrier season rankings will be posted after the Foot Locker national championships Dec.8.
LaSalle's Allen Bader won his second consecutive Division I state title Nov.3. Last weekend Bader finished second in the Mid-East Meet of Champions, an all-star race for seniors held in Kettering, Ohio.
LaSalle was ranked No.1 nationally earlier this season but finished second to Hudson in the Ohio Division I state meet.
HOOP SCOOP: The Enquirer's annual boys basketball preview will run Friday, the opening day of the season.
FOOTBALL STARS: The OHSAA released its final list of regular-season football statistical leaders, with all divisions grouped together. Williamsburg's Jason Bainum led the state in rushing (3,248 yards) and scoring (284 points on 43 TDs, 13 two-point conversions).
Wyoming's P.J. Pope (194 points) and Mount Healthy's B.J. Flowers (192) ranked Nos.3-4 in scoring.
North College Hill's Donnie Johnson was No.3 in rushing (2,227 yards), with Flowers at No.5 (2,187 yards) and Reading's DeShawn Wynn at No.9 (1,917).
McNicholas' Bryan Cupito was No.3 in passing yards (2,973) and Princeton's Mike Daniels ranked No.9 (2,360).
Princeton's Darren Barnett was No.3 in pass receptions (81).
COACH HONORED: Williamsburg coach Ed Stewart has been awarded the Ohio High School Volleyball Coaches Association Lifetime Achievement Award.
Stewart, volleyball coach at Williamsburg for 23 years, has a record of 431-97 and has been named Southern Buckeye Conference coach of the year 14 times.
COACHES NEEDED:Walnut Hills seeks a head football coach. Apply via mail only with cover letter, resume and three letters of recommendation to: Principal, Walnut Hills High School, 3520 Victory Parkway, Cincinnati, OH 45207. No faxes, e-mails or phone calls accepted. Deadline is 5 p.m. Friday.
Walnut Hills also needs a varsity boys track coach for the spring season. Call AD Matt Szyndler at 569-5540.
Turpin seeks a head football coach. Send resume to AD Tony Hemmelgarn at Turpin HS, 2650 Bartels Road, Cincinnati, OH 45244.
Contact Tom Groeschen at 768-8450; fax: 768-8550; e-mail: firstname.lastname@example.com.
Dillon key to Battle of Ohio Browns favored for first time Balanced offense worked the first time Who's got the edge? Players to watch Bengals-Browns by the numbers NFL power ratings Today's NFL predictions St. X rolls into state finals DAUGHERTY: St. X keeps our hopes up Dream lives on for St. X brothers Ticket sales fall short Sollmann brothers recall playoffs Ohio football playoffs Ohio playoffs roundup CovCath ousted in state semifinal 17-14 Kentucky football playoffs Indiana football playoffs Last-second TD pass puts Bearcats in bowl UC lineman embraces Russian heritage, but not food UC 74, UNLV 61 Little sheds goggles, sees the light UC women 65, Middle Tennessee 63 UC women lose in soccer Sweet 16 Missouri 72, Xavier 60 Missouri star shows he's the real deal Xavier women lose again Larkin recovery on track Reds Q&A with John Fay


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Female Hippy Costume (ref: 69783)
Reviews (0) |- You'll bring some much needed flower power to your next 60s themed party in this kaleidoscopic costume.
- Includes a multicoloured dress printed with flowers and peace symbols, and a matching headband.
- Excludes peace necklace, earrings and boots.
- The dress features dramatic bell sleeves and a v-neck front to create a classic hippie appearance.
- Available in - Small: Dress size 10 / Medium: Dress size 12 / Large: Dress size 14 / Extra Large: Dress size 16


text:
Route Grade Votings
Total votes cast 16
Adjacent Routes The thin grey wall give some technical climbing between welcome rest positions. A separate anchor from Sapfo, but no clip gate. USER COMMENTS
...mmmm 7a is to much, is an hard 6c+...very technical
7a may be justified? thin and technical through the crux. regardless of grade its superb.
The hardest 7 a I did in Kalymnos. Fierce to on-sight |


text:
Campbell Hill Additions and Corrections
[ Post an Addition or Correction ]
Alpinist | Accessible Hours | |
Voted 9/10 | Campbell Hill is now open on Saturdays all year round from 7am - 7pm. Call (937) 599-3010 in advance to make arrangements for access on Sundays. | |
Posted Jul 13, 2007 9:14 am |
hgrapid | Thank you | |
Hasn't voted | Thanks for the update. Added. | |
Posted Jul 13, 2007 4:06 pm |
Redwic | There are two gates... | |
Hasn't voted | A small person-only gate is found to the left of the main (drivable) gate. It is open to the public more often than not, regardless if the main gate is closed/ locked. If arriving outside of the "normal" operating hours, climbers should call the school's number provided on this website (937-599-3010) in advance, to verify the person-only gate will still be open. | |
Posted Jul 7, 2008 12:09 pm |
hgrapid | Re: There are two gates... | |
Hasn't voted | How is this different or add to what I already note on the website? Am I missing something in the Red Tape section that would confuse people? | |
Posted Jul 7, 2008 4:14 pm |
Redwic | Re: There are two gates... | |
Hasn't voted | It's worth mentioning there are actually two gates, one specifically for walk-ins only, so that a highpointer doesn't get upset or worried if the main (drivable) gate is closed. In example, I called in advance of this past weekend. The main gate was supposed to be open on Saturday, but wasn't. Nobody was around. At least when I called/ double-checked in advance, the school just happened to also mention the walk-in only gate. This HP Summitpost page did not reference it, and the walk-in gate is not immediately noticeable. I think the walk-in only gate (to the left of the main gate) should be mentioned, just in case of a situation like what I had (where things were supposed to be a certain way but ended up differently). | |
Posted Jul 7, 2008 8:56 pm |
hgrapid | Re: There are two gates... | |
Hasn't voted | Okay, thanks | |
Posted Jul 7, 2008 9:36 pm |
sm0421 | Time and parking | |
Hasn't voted | It's about 86 miles from camp hill to Hoosier, I had a plane to catch and i was driving extremely fast and passing any car i can on country road. I drove almost 80 when i can. It took me an hour and half to get to Hoosier. So i think it should take a regular person about 1 hour 40 minutes to 2 hours, instead of the 'a little over an hour ' on the page. Also the parking area is after you see the big career center sign, turn right into the school campus. | |
Posted Jan 20, 2011 5:58 pm |
sm0421 | thanks! | |
Hasn't voted | hgrapid, Thank you for the updates on the Campbell Hill page, I spent so much time reading this page, thanks for the effort maintaining it for future highpointers... Warm Regards, Alex | |
Posted Jan 21, 2011 8:52 pm |


text:
• Banning the procedure at publicly funded facilities is among several issues being discussed as budget amendments.
COLUMBUS, Ohio (AP) — Abortions in Ohio would be banned at most publicly funded hospitals, clinics and other facilities under a proposal Senate Republicans are considering putting in the state budget, a Senate GOP official told The Associated Press on Monday.
The official, who had direct knowledge of the changes being discussed, requested anonymity because the revisions were still a part of negotiations.
State senators also are weighing a separate amendment to restrict public employees' insurance from covering abortions, the official said. Not included would be those public employees of the state's two chartered counties and chartered municipalities, which account for roughly two-thirds of the cities in the state.
Abortions in the case of rape or incest or saving the life of the mother would be exempted from both proposals, the official said.
The revisions come as the Republican-led Senate Finance Committee is scheduled today to review additional amendments to the $55.7 billion, two-year spending blueprint and is likely vote on the measure.
Other changes being discussed would require the General Assembly to sign off on any private vendor that would take over the day-to-day management of the Ohio Lottery Commission. Senators also are considering stripping the bill of an idea to open up the state's parks to oil and gas drilling, though separate legislation on that issue is making its way through the Legislature.
Amendments offered by the governor's office would remove Gov. John Kasich as the chairman and director of a new semi-private economic development panel and make other adjustments to the entity.
Kasich, a first-term Republican, would still have the power to pick the nine members of the JobsOhio board.
Two Democratic state lawmakers and the liberal policy group ProgressOhio have filed a lawsuit against JobsOhio, claiming the job-creation entity violates seven sections of the Ohio Constitution. The lawsuit, which is before the Ohio Supreme Court, says it's unconstitutional for Kasich to take a second position of authority for the state while serving as governor.
The changes are not in response to the lawsuit, Kasich spokesman Rob Nichols said Monday.
"We're committed to JobsOhio's independence, transparency and accountability and by working with the General Assembly, we've been able to craft revisions that improve it on all of those fronts," he said in a statement.
Brian Rothenberg, executive director of ProgressOhio, says the revisions validate the group's concerns with JobsOhio.
"While the governor's office and the General Assembly have not addressed all of the constitutional issues we argue in our case, the actions they have taken prove that we were correct in our original findings," Rothenberg said in a statement.
Republicans in the Ohio Senate also plan to introduce a state budget amendment that would cut lawmakers' base salaries by 5 percent.
The state constitution prohibits a legislator's salary from being changed during his or her term, so the cut could not take effect until the start of the next General Assembly. In the meantime, lawmakers could take the pay cut voluntarily.
If passed, the cut would be the first major change in lawmakers' pay since 2008, which marked the end of a phased-in pay raise that began in 2002.
That increase has left the base salary for a senator at just under $60,600, an amount that doesn't include thousands of dollars lawmakers can earn for serving in leadership roles in the chamber and its various committees.
A vote by the full Senate could come as soon as Wednesday. The House passed its version of the budget last month.
Lawmakers in both chambers would have to work out any differences between the two budget plans in what's called a conference committee. The deadline for them to pass the state spending plan is June 30.


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Otaki Lightweight Pullover
A Fusion of Science and Fashion-Forward Style
Canadian-based Sunice is fast becoming one of the leading manufacturers of golf outerwear in the United States and as such was just a few short years back selected by the LPGA to design, manufacture and provide the waterproof outerwear that was worn by the 2011 and 2013 U.S. Solheim Cup teams. Their performance-enhancing golf and ski outerwear uses advanced technologies, fabrics and manufacturing methods to deliver Best-In-Class outerwear with complete freedom of movement.
The Sunice Sport Layering collection is a culmination of all Sunice styles that speak to lifestyle layering pieces and the Otaki lightweight pullover is one of their most popular and proven new releases. With its combination of science and style in a soft shell, and with lightweight layering properties the Otaki pullover is sure to set you in style, and in motion, on the course or off and looking great.
- Highly functional lightweight pullover
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- 78% Polyester/22% Spandex
- Style: 5613
GolfDiscount.com is proud to use FedEx as its primary shipping provider. All packages shipped via FedEx include shipping insurance as part of the quoted price. Packages are protected 100% until the merchandise arrives and is signed for by you. We offer FedEx Ground Shipping to the Contiguous 48 states. All FedEx shipment times are quoted in business days, as neither FedEx Next-Day service nor FedEx 2-Day service ship or deliver on weekends or holidays.
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text:
by Britta White, University Of Richmond
This WebQuest is designed to reinforce the contents of the Virginia Department of Education's Standard of Learning 3.5 Number and Number Sense - Fractions.
There has been several thefts in Whole Town and Part City. The two of you have been assigned as the detectives on the case of The Mysterious Fraction Thieves. You'll have two days to solve the crime.


text:
Society kicked on me, hit on me til I was sick and exhausted
from Crooked I (Ft. Slaughterhouse) – Monsters In My Head Lyrics on Genius
"They" refer to society and/or music industry. He never fully released an album since he obtained his first record deal in 1995.
— 2000: Untouchable (un-released)
– 2002: Say Hi To The Bad Guy (un-released)
– 2010: Hood Star *
Now that he's with Shady Records, things are looking up though.
*Hood Star is a compilation of unreleased tracks.
To help improve the quality of the lyrics, visit Crooked I (Ft. Slaughterhouse) – Monsters In My Head Lyrics and leave a suggestion at the bottom of the page


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- Northern New York Newspapers
- Watertown
- Ogdensburg
- Massena-Potsdam
- Lowville
- Carthage
- Malone
- NNY Business
- NNY Living
- NNY Ads
ASSAULT - MASSENA - Village police charged Christopher H. Pettitt, 24, of 88 Bayley Road, Massena, with third-degree assault fat 9:45 p.m. Wednesday following an incident that took place at his residence. He reportedly bit Sandra Moerlin on the lip during a domestic dispute. He was arraigned by Massena Village Justice Eric J. Gustafson and sent to the St. Lawrence County Correctional Facility with bail set at $1,000 cash or bond.
Ms. Moerlin, in her statement to police, said Pettit had several drinks and was upset that his daughters friend was at their residence. Chris got upset with me, saying I never listen to what he has to think. Chris pushed me and wouldnt let me leave the bedroom, she noted in her statement to police.
She said her 12-year-old daughter then intervened, and Petitt started yelling at her. Ms. Moerlin said she got between Pettit and her daughter and told her boyfriend to leave the residence. Chris wouldnt leave. Then Chris grabbed both my arms and bit my lip, she told police.
Ms. Moerlin was taken to Massena Memorial Hospital for treatment of her injury.
The domestic incident report indicated Pettit had admitted consuming six to seven Twisted Ice Teas before the incident. The report indicated Ms. Moerlin had left a blood trail in her bedroom and the upstairs hallway from her injury.


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very helpful
The fact that it was right off the highway.
there were no elevators. The camera was not working to the entrance of the motel and someones car got broken into. not enough breakfast for people to eat and they only put out a little at a time then they just completely ran out, no roll away bed
The location was perfect for the thingsmwe needed to do and it was right off the interstate. Staff was also firendly.
Room service didn't come by at all during our stay so the bed was not made and towels weren't replenished. Also had to ask for more towels because they only gave us two of each. Door key also had to be replaced 3x because iof bad keys.
It was convenient to my gathering.
The pool was not in service. Was told that it would be available next year. When we arrived there were 'workers' parked in the guest parking spots. Breakfast left a lot to be desired.
Here's why:
We're not the seller At KAYAK we don't set prices, so it's not possible for us to guarantee what other companies are selling.
We aggregate tonnes of data for you Our service lets you quickly and easily compare results from hundreds of travel sites at once. In this sense, KAYAK is a search engine and we don't guarantee prices we find.
Why aren't prices accurate 100% of the time? Prices on airline seats, hotel rooms and car hires can change frequently. Several people may also be trying to buy the same travel option simultaneously. As a result, you may find on occasion that certain prices are no longer available.
More questions or if you have a bad price to report to us Please send us an email and someone from our team will get back to you promptly.


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recipe reviews for:
"Perfect for chocolate lovers! Serve this dense and fudgy cake dusted with confectioners' sugar, glazed with ganache, or with whipped cream or ice cream."
My Profile | Cooks I Like Reviews | Photos | Recipes | Menus | Blog
Cooking Level: Expert
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A simple tortilla topped with Mexican fillings gives you a burst of spicy flavor.
Flan, churros, tres leches cake? We have these and so many more. It's fiesta time!
Delicious recipes, party ideas, and cooking tips! Get a year of Allrecipes magazine for $7.99!
See how to make an easy, fudgy, gluten-free chocolate cake.
This dense chocolate cake is perfect for those who avoid flour or gluten.
This quick-and-easy chocolate cake is so moist and rich.


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The President of the United States in the name of The Congress takes pleasure in presenting the Medal of Honor to
Carter, Ty Michael. Rank and organization: Staff Sergeant, U.S. Army, Troop B, 3d Squadron, 61st Cavalry Regiment, 4th Infantry Division. Place and Date: Combat Outpost Keating, Kamdesh District, Nuristan Province, Afghanistan, 3 October 2009. Entered Service at: Antioch, California. Born: SEptember 1980, Spokane, Washington Citation: For acts of gallantry and intrepidity at the risk of his life above and beyond the call of duty while serving as a Scout with Bravo Troop, 3d Squadron, 61st Cavalry Regiment, 4th Brigade Combat Team, 4th Infantry Division, during combat operations against an armed enemy in Kamdesh District, Nuristan Province, Afghanistan on 3 October 2009. On that morning, Staff Sergeant Carter and his comrades awakened to an attack of an estimated 300 enemy fighters occupying the high ground on all four sides of Combat Outpost KEATING, employing concentrated fire from recoilless rifles, rocket propelled grenades, anti-aircraft machine guns, mortars and small arms fire. Staff Sergeant Carter reinforced a forward battle position, ran twice through a 100 meter gauntlet of enemy fire to re-supply ammunition and voluntarily remained there to defend the isolated position. Armed with only an M4 carbine rifle, Staff Sergeant Carter placed accurate, deadly fire on the enemy, beating back the assault force and preventing the position from being overrun, over the course of several hours. With complete disregard for his own safety and in spite of his own wounds, he ran through a hail of enemy rocket propelled grenade and machine gun fire to rescue a critically wounded comrade who had been pinned down in an exposed position. Staff Sergeant Carter rendered life extending first aid and carried the Soldier to cover. On his own initiative, Staff Sergeant Carter again maneuvered through enemy fire to check on a fallen Soldier and recovered the squad's radio, which allowed them to coordinate their evacuation with fellow Soldiers. With teammates providing covering fire, Staff Sergeant Carter assisted in moving the wounded Soldier 100 meters through withering enemy fire to the aid station and before returning to the fight. Staff Sergeant Carter's heroic actions and tactical skill were critical to the defense of Combat Outpost Keating, preventing the enemy from capturing the position and saving the lives of his fellow Soldiers. Staff Sergeant Carter's extraordinary heroism and selflessness above and beyond the call of duty are in keeping with the highest traditions of military service and reflect great credit upon himself, Bravo Troop, 3d Squadron, 61st Cavalry Regiment, 4th Brigade Combat Team, 4th Infantry Division and the United States Army.
Citation Courtesy of www.HomeOfHeroes.com
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text:
We are headed North this time
About this blog
Pat, Sarah and I are off on a two week tour of the North East. If you want to follow along I will try to pass on my observations of our trip every day or so. I will also be posting some pictures if you are more of a visual arts person.
Any way the blog is here for your enjoyment or to practice the use of your delete button.
Trip Start
Jul 16, 2011
Trip End
Jul 31, 2011


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Noise and nuisance
This cross-cutting theme is now closed for comments.
You can read comments made since the start of the Red Tape Challenge in April 2011 below.
You can also still submit comments to the Red Tape Challenge inbox by clicking here.
These regulations aim to limit the impacts from noise, other nuisances such as litter and dog fouling; and reduce the effects of noise from roads, railways and airports. In some cases the regulations include provisions to issue fixed penalty notices, and require authorities to conduct reasonable investigations.
You can find all 25 regulations that relate to noise and nuisance below to the left.
Tell us what you think should happen to these regulations and why, being specific where possible:
- Should we scrap them altogether?
- Could their purpose be achieved in a non-regulatory way (eg through a voluntary code?) How?
- Could they be reformed, simplified or merged? How?
- Can we reduce their bureaucracy through better implementation? How?
- Can we make their enforcement less burdensome? How?
- Should they be left as they are?
For example parts of the Control of Pollution Act 1974 are largely unused, perhaps you think they can be scrapped.
121 responses to Noise and nuisance
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Ken Patrick said on September 21, 2011 at 7:27 pm
It is time to do something about the noise levels from motorcycles. The blatant/misuse of track only 'cans' and big bore exhausts on cars is getting way out of hand. Sundays where I live are accompanied by the all-day chorus of screaming motorcycle exhausts, boom of big bore exhausts and the boom chukka boom of the max'd out bass of a cars ICE system.
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Nick Marks said on September 21, 2011 at 12:17 pm
On a purely legal basis, looking at the Regulations under consideration, they fall into two categories: EU Regulations and UK Regulations. Given that the EU Regulations are part of the responsibility of the UK government to implement as part of its responsibilities as a member state, it would appear that we could not opt out of EU regulations (even if we wanted to) without leaving the EU. I believe that many of the UK regulations also derive from this source.
With respect to noise, removing the duty of a local authority to investigate under S79 & 80 would be unpopular with the public given the levels of domestic noise complaints. As for S101 & 2 which introduced insects and light as statutory nuisance issues, this was done following public pressure as there was no previous remedy in law for these nuisances.
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Liam Meanwell said on September 19, 2011 at 8:48 pm
I would give the powers to deal with noise complaints back to the police instead of a local council. The council aren't much help at 2 in the morning when you are being kept up and my council (Waverley borough council) haven't followed up on any out-of-hours complaints.
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Kyle Marriott said on September 19, 2011 at 1:45 pm
Clear measurement based legislative definition regarding limits, rather than the current subjective definition.
Tighter controls over property development near to pre-existing venues to prevent long established businesses being forced to close through no fault of their own.
Currently many venues in and around the north west have been put into difficult situations due to new build flats cropping up within a few hundred metres of their doors, seemingly built with absolutely no regard for sound proofing or prevention of noise bleed.
Also I second the comments regarding a specific qualification being required for noise abatement officials to operate. I myself have dealt with officers on multiple occasions who do not even have a meter on their person, and refused to work with my staff's own on-site metering equipment to reach a happy medium that would allow the event to continue whilst minimising disturbance to locals.
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Mike Sealey said on September 19, 2011 at 1:22 pm
I have lost significant work because of noise abatement orders imposed upon music venues, through complaints from residents who moved in AFTER the premises began operating as a live music venue.
Would I be offered the same sympathy if I moved in next door to Gatwick airport and complained of noise?
I believe noise abatement orders should be more transparrent. I believe the complainer must publicly state the disturbance, allowing the cause of the problem to offer a solution. I also believe that in the case of music or entertainment venues, anyone who wishes to make a complaint should only be allowed to do so if they have been living in the area for longer than the cause of the disturbance has been operating. Finally, anyone who wishes to work on behalf of the council should have a qualification recognised among industry professionals. I have dealt with an employee of the council working in Environmental Health who was not aware of the existance of multiple scales of weighting on audio-measurements. Such school-boy errors are not acceptable for someone who has the power to shut down businesses.
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June Rovai said on August 9, 2011 at 9:22 am
Legislation on noise nuisance could be simplified but at the same time made more stringetn and easier to enforce. We constantly hear what is being done to alleviate/deal with noise nuisance in social housing but nothing is heard about trying to improve the life of those in privately owned property who suffer from constant noise nuisance from neighbours. Enforcement appears to be almost impossible especially when there is often a lack of will on the part of local Environmental Health Officers.


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The Center for Internships and Career Placement provides a variety of career development and job search services for Regis College alumni. These services include career counseling and assessment, assistance with resumes and cover letters, tools you can use to conduct an effective job search as well as access to other alumni and graduate school advising. Learn more.
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text:
Stratford University has selected EthicsPoint, Inc. to provide a simple, secure way of anonymously and confidentially reporting activities that may involve unethical or otherwise inappropriate conduct in violation of University policies.
Stratford University considers employee concerns very important and desires your cooperation in resolving such issues. This hotline service does not replace existing reporting methods on campus. Employees are encouraged to bring any concerns to their supervisors, Human Resources, or other campus entities as appropriate. EthicsPoint merely provides another outlet for reporting ethics issues, particularly when the reporter's anonymity is essential.
Stratford University asserts that reports submitted via EthicsPoint will be handled promptly and discretely. No retaliatory action will be taken against anyone for reporting or inquiring in good faith about potential breaches of Stratford University's policies, or seeking guidance on how to handle such breaches. Reports submitted through EthicsPoint will be given careful attention with the objective of correcting the situation being reported, if facts can be substantiated. Employees are expected to use good judgment in the use of this reporting system.
You may use either of the following two methods to submit a report:
After you complete your report you will be assigned a unique code called a "report key." Write down your report key in a safe place. After 5-10 business days, use your report key and password to check your report for feedback or questions.
Do not use this site to report events presenting an immediate threat. Reports received through this service will not receive an immediate response. If you have an emergency, please contact Stratford University's Safety and Security Office or the local authorities.


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TAUNTON, Mass. – The South Eastern Economic Development (SEED) Corporation recently made a micro loan to Newport Potters Guild to enable the small business to start a pottery and clay art gallery and create four new jobs.
By PBN Staff | 6/16/09 |
Dennis M. Hanno has been working in his new capacity as president of Wheaton College in Norton for nearly three months, and will be inaugurated Oct. 17. He will then begin charting the school's future under his tenure. Profiled in his college's quarterly as devoted to hard work and leadership, he has inherited the school's liberal arts approach to higher education and some common challenges.
By Patricia Daddona |
City gamer Catherine Brunzos is the "Minecraft Hunger Games" World Champion after taking part in a weekend International Games Day event at Providence Community Library.
By Lori Stabile |
(Updated Feb. 24) Rhode Island's teacher preparation policies are among the strongest in the country, earning the Ocean State an overall grade of B+, according to the National Council on Teacher Quality.
By PBN Staff
Providence attorney Matthew Parker is familiar with taking depositions, but a new program that debuted in mid-November gave him a crash course in deposition techniques that he'd been eager to try.
By Patricia Daddona |
Dr. Deborah Riebe, professor and chair of the kinesiology dept. at the University of Rhode Island, has been nominated as to act as the senior editor for the 10th edition of the "American College of Sports Medicine's Guidelines for Exercise and Prescription." The book is considered an industry standard and it is used as a reference by clinical exercise, public health and other health care professionals for treating both healthy individuals, as well as those with a chronic disease or condition. The handbook is also used as a supplementary textbook and as a resource for professionals preparing for the ACSM exam. She holds a B.S. in physical education from Springfield College, an M.S. in exercise science from the University of Rhode Island and a Ph.D. in exercise physiology from the University of Connecticut.
As health care changes hit the workplace, nurse managers and paramedics have the opportunity to obtain more academic credentials and training here in Rhode Island this year and in the near future.
By Patricia Daddona |
Johnson & Wales University's Ray McCue, an assistant professor, has been named best culinary educator in the Northeast.
By Patricia Daddona |
Rhode Island College's R.N. to B.S.N. program, which allows registered nurses to earn bachelor of science in nursing degrees, is the 10th best in the country.
By PBN Staff
With help from a Verizon Innovate Learning Grant, sixth- and seventh-graders at The Learning Community in Central Falls will learn to build their own robots and create software applications.
By Lori Stabile |


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Battle of Mygeeto (Galactic Civil War)
this wiki
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The Battle of Mygeeto was a battle during the Galactic Civil War.
After a massive Rebel uprising on the planet Mygeeto, a fleet of Imperial warships, personally commanded by Darth Vader, prepared to re-capture the world.[2]
The battleEdit
The battle itself was split into three sections. The first phase began with the deployment of multiple TIE fighters squadrons to perform reconnaissance runs and combat incoming Rebel X-wings.[2]
The second phase moved the aerial battle into the Mygeeto asteroid field, where Rebel scouts had been discovered. The Rebels conducted a counter-offensive, which saw a climatic battle between the main Imperial fleet and the remnant Rebel fleet.[2]
After hours of ship-to-ship fighting, the Empire emerged victorious, which allowed a clear attack run and ground assault on the planet. The Imperial fleet's TIE Bombers proceeded to bombard strategically important targets, including ion cannons, shield generators and the main spaceport. Meanwhile, Lord Vader led an assault with AT-ST units, which led to Mygeeto's quick re-capture.[2]
- Star Wars: Imperial Ace (First appearance)
Notes and referencesEdit
- ↑ The inclusion of TIE/In interceptors, TIE Defenders, and A-wings in the game implies it is likely set after the Battle of Hoth, since these craft were not introduced into service until after that battle.
- ↑ 2.00 2.01 2.02 2.03 2.04 2.05 2.06 2.07 2.08 2.09 2.10 2.11 2.12 2.13 2.14 2.15 2.16 2.17 2.18 2.19 2.20 2.21 2.22 2.23 2.24 2.25 2.26 2.27 2.28 2.29 2.30 2.31 2.32 2.33 2.34 Star Wars: Imperial Ace
- ↑ The Essential Atlas


text:
Applications for Christmas assistance must be made in person at the sheriff's office.
The following documents will be required to apply:
1. Photo I.D. of parents or guardians
2. Social Security cards
3.Paycheck or unemployment check pay stub for both parents
4.SSI, Disability, or food stamp case numbers or letters
5.Child support, alimony should be documented
6.Proof of Marshall County residency
7.Rental contract
8.Legal guardianship papers
9.Birth date of each child
Children 15 and older are not eligible for assistance.
Monetary donations and toys will be gratefully accepted at the office from 8 a.m. to 4:30 p.m. Monday through Friday.


text:
1-11-18 Shitennoji, Osaka, Japan 543-0051Map
+81 6 6771 0066
Neighborhood: Tennoji Area
Shitenno-ji Temple is one of the oldest temples in Japan. The original buildings were constructed over 1,400 years ago at the behest of Prince Shotoku. The temple is especially known for the arrangement of its buildings in a straight north-south line - an arrangement that has come to be known as 'Shitenno-ji-style temple layout'. The outstanding symmetrical balance of Shitenno-ji's architecture has been greatly admired over the centuries. The temple was destroyed several times in fires caused by lightning and war, but has always been rebuilt.
Write a Review
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- Open Hours: Tuesday to Sunday from 08:30 AM to 04:00 PM
- Type:
Attractions & Landmarks, Religious
- Nearest Train: Shitennojimae-yuhigaoka Station
Hotel Metro The 21
2.0 mi
Shin-Osaka Sunny Stone Hotel
2.0 mi
Hotel Sunlife
2.0 mi
Hotel Nikko Osaka
2.0 mi
Dai-ichi Osaka Hotel
2.0 mi
See All Osaka Hotels »
Tennoji Park
0.5 mi
Tennoji Zoo
0.5 mi
Tsutenkaku Tower
0.6 mi
0.6 mi
Municipal Museum of Fine Art
0.6 mi
See All Osaka Things To Do »
See All Osaka Restaurants »
See All Osaka Nightlife »


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Regions :: India/Pakistan Bayer CropScience and Food Company Reitzel to Expand Collaboration in India12:02 AM MST | February 14, 2011 | Deepti Ramesh Bayer CropScience says it plans to expand its collaboration with food company Reitzel (Aigle, Switzerland) on the sustainable production of high-quality gherkins to all contract farmers of Reitzel India. Both companies have been working together in a food chain partnership project since 2009. In this partnership, Bayer CropScience offers Reitzel and Indian farmers crop protection solutions and technical expertise. Nunhems, Bayer CropScience's vegetable seed business, provides Ajax, a high-yielding hybrid gherkin variety. Reitzel's agri-technicians... This information is only available to Chemical Week subscribers. Forgot your user ID or password?
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text:
Just Call Me The King
Hey! DDP and boxesNovember 21st 2011 1:55 pm[ Leave A Comment | 2 people already have ]
Furries, Leave A Comment | 2 people already have You tell them, King Henry! Have a safe move!!! conga ratz two ewe buddy on yur dia ree a cheese mint !!! pretty soon yur toys will be back outta de boxes then ewe can play with both !!! haza safe mewve :) |
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§ Mr. MorganTo ask the Secretary of State for Energy when he expects to make a decision on the next stage of funding for the Mersey barrage and its associated design and study programme.
§ Mr. MoynihanThe current phase of the studies is due for completion in July. A decision on work will depend on the assessment of the results of the current phase.
§ Mr. MorganTo ask the Secretary of State for Energy what representations he has received concerning his decision-making timetable and other aspects of public funding for the Mersey barrage.
§ Mr. MoynihanMy right hon. Friend and I have received a number of representations on a variety of aspects of work on the proposed Mersey barrage system.


text: I know exactly what you mean. I'm feeling less like a pig now that my stomach is stretched because all the eating is happening at meal times instead of trying to feed all day to get the calories in. I also realize that now that I don't feel like I'm going to burst from the food it doesn't seem like I'm pigging out, I'm more able to see how it's still not an unusual looking amount of food, just more than I was used to. People must have thought I was just eating small bits in front of them and pigging out later!