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March 31, 2014 | Mushroom Cultivation, Mushrooms | 5 comments | Author: Kirsten Bradley
As if growing your own mushrooms wasn't excellent enough, there's also a way you can further increase their Vitamin D content… simply by putting them in sunlight.
And not increase that Vitamin D by a little bit, either. We're talking a bigtime boost in nutrition here.
This technique applies at least to shiitake mushrooms (our favourite) and button mushrooms, but may also apply to other types of mushrooms too.
But shiitakes and button (field) mushrooms are the ones that have been most studied on this front, so let's go with what we know.
We first heard about this technique via the fabulous Paul Stamets of Fungi Perfecti.
And to us, it just goes to show yet another level of amazing that the world of mushrooms has up it's sleeve (or gills, I suppose).
So – the summary is this:
When exposed to sunlight for two days, shiitake and button mushrooms inherently increase their Vitamin D content.
And it's not a small increase. Their Vitamin D content soars. To 460 times that of a non-sun-exposed mushrooms.
The best bit? It's not only fresh mushrooms that seem to take on this increase in Vitamin D. Dried mushrooms can also increase rapidly in Vitamin D if previously not exposed to sunlight.
– According to Paul Stamets, 2 days of sunlight exposure is the sweet spot. After that period of time, the Vitamin D decreases from it's highest levels, possibly due to over exposure to sunlight.
– Sliced mushrooms gain more Vitamin D in this process than whole mushrooms, due to increased surface area exposure.
– As said above, you can do this with pre-dried mushrooms as well to increase their Vitamin D content (although I couldn't find any info on the exact results of this, only that the levels increased)
– Placing the shiitakes under a UV light for the same period will have the same effect, but sunlight is footprint-free.
– Apparently a small handful of these (10g) four times a week is equivalent to all the Vitamin D a vegan needs to thrive, so take note, meatless folk!
– Vitamin D is one of those vitamins you can take too much of if you try hard enough, so don't eat kilos of these every day. Not that we think you would.
We went with mostly whole mushrooms, partly because i'm so in love with the idea of having jars of whole dried home grown shiitakes in our kitchen.
For the rest of the drying process, we used a few methods.
Mostly we dried them whole (little ones) and sliced (big and wonky ones) in our excalibur food dehydrator which continues to be an awesome tool for preserving the harvest.
We also dried a bunch of sliced shiitakes in the bottom of the woodstove, because it was lit one night. Free heat energy is not to be sniffed at, I figure.
A great stash of home-grown, organic sustenance for winter and beyond.
And now, with enhanced Vitamin D!
Growing organic shiitakes is one of the many indoor and outdoor cultivation techniques covered in our awesome 2-day Gourmet Mushroom Cultivation courses, which happen in Sydney every 3 months.
So you can enhance then store and the Vit. D levels aren't damaged with storage? I find it interesting that I don't even like mushrooms but I have been totally fascinated by them lately. I have even been considering if I could sell them even though I wouldn't eat them! LOL
I read that… gills up if I remember and it's true of all mushrooms.
Reblogged this on permintic health & wellness.
so the two days dose – do you even need it in a field grown mushrrom? and is this greatlyinfluenced by latitude? We don't get a lot of UVB in Tassie after april
[…] week, Kirsten has another post about shiitake cultivation and increasing the vitamin D content of the mushrooms by putting them in the sun. People yes…but mushrooms! Who'd a thunk […]


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We will delivery to all cities in
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subset: commoncrawl


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Drama series for four- to six-year-olds about the parallel lives of parents at work and their children, who stay with warm-hearted minder Granny Murray.
The children are going to a pirate party but when Jordan, whose party it is, turns up at the hospital with a broken arm, Dr Juno makes sure that he can still dress up as a pirate - even in a cast!
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I've been posting a little here and there for a couple of weeks but I wanted to wait until I had a picture of my truck to share for a "proper introduction".
I've owned two pickup trucks before and my last vehicle was a leased (thankfully) H3. I've been wanting a pickup again since I started driving the H3 and I've had my heart set on a Tacoma for the last 3 years. I FINALLY got my Taco on June 23!!!
With the exception of the removal of the front mud flaps, the truck is completely stock for now. But at some point I plan on upgrading tires/wheels/suspension and a few other things.
Any ways, I live up in the Seattle area. Any other fellow NW Taco owners out there?
And a couple of pictures of my truck (I'm sure it's nothing you haven't seen before):
BTW, this is a great site!


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The Library
Regularity effects and the phonological deficit model of reading disabilities : a meta-analytic review
Metsala, Jamie L., Stanovich, Keith E., 1950- and Brown, G. D. A. (Gordon D. A.) (1998) Regularity effects and the phonological deficit model of reading disabilities : a meta-analytic review. Journal of Educational Psychology, Vol.90 (No.2). pp. 279-293. ISSN 0022-0663
Full text not available from this repository.
Official URL: http://dx.doi.org/10.1037/0022-06220.127.116.119
This article presents a meta-analysis of spelling-to-sound regularity effects in individuals with reading disabilities and reading-level comparison groups. The phonological-deficit model of reading disabilities, coupled with the classic dual-route model of word recognition, has led to two predictions: (a) a specific deficit in the pseudoword reading of those with reading disabilities and (b) an absent or reduced regularity effect for those with reading disabilities relative to reading-level controls. Previous reviews confirm the first prediction. The present meta-analysis tested the second prediction. There was a clear effect of word regularity for individuals with reading disabilities, the magnitude of which did not differ from the word regularity effect for reading-level controls. The authors explore how the contradictory support for these 2 predictions is inconsistent with classic dual-route models of word reading and how connectionist models are consistent with the empirical findings on reading disability.
Item Type: | Journal Item |
Subjects: | B Philosophy. Psychology. Religion > BF Psychology L Education > LB Theory and practice of education P Language and Literature > P Philology. Linguistics |
Divisions: | Faculty of Science > Psychology |
Library of Congress Subject Headings (LCSH): | Reading disability, Phonetics, Cognition, Meta-analysis |
Journal or Publication Title: | Journal of Educational Psychology |
Publisher: | American Psychological Association |
Official Date: | June 1998 |
Volume: | Vol.90 |
Number: | No.2 |
Number of Pages: | 15 |
Page Range: | pp. 279-293 |
Identification Number: | 10.1037/0022-06184.108.40.2069 |
Status: | Peer Reviewed |
Publication Status: | Published |
Funder: | National Reading Research Center (U.S.) (NRRC), United States. Office of Educational Research and Improvement (OERI), Natural Sciences and Engineering Research Council of Canada (NSERC) |
Grant number: | 117A20007 (NRRC) |
URI: | http://wrap.warwick.ac.uk/id/eprint/15635 |
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Yet SC has much cheaper gas than NJ, and no toll roads. All states have a heavy gas tax, and the feds take an even larger chunk. I used to work in the oil and gas industry.
Toll roads are BS. NJ, VA, and PA all have many toll roads, and these roads seem to be maintained just as poorly as the non toll roads. Then there are the bridge tolls (Delaware to NJ = rape).
In regards to paying $8-$12 per day to use the beach, I think that is asinine. I don't think the beach communities should get any more assistance from the state or feds if they are charging for use. Why should I have to see my taxes go toward their maintenance, when I still have to pay to use it? The largest cost of beach maintenance is restoration. The only reason there is restoration, is because wealthy people built houses right up to the water.
As if I needed more reasons to dislike NJ...


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03-23-2014, 06:24 PM | #1 (permalink) |
Junior Member
Join Date: Mar 2014
Location: Okinawa Japan
Posts: 26
Please Help orange pee
Hey everyone so i'm a new GSD mama and just having a neurotic time with my Phoenix. So When I got her she had Diarrhea for 1 week and took her to the vet twice second time they found Coccidia parasite. Now that she's all treated for that and having normal poops. I noticed last night that she has a very pale orange pee. It's not every pee maybe 2 orange one yellow. This morning i'm calling the Vet again he probably hates me. But in the mean time I am wondering if anyone has any insight. I have water always available and this morning she also puked cat poop so i'm wondering if that has anything to do with it. She's eating and acting her normal self.
She's eating Science Diet I tried giving her Blue Buffalo but I think it's too rich for her because she gets sick after eating it. |
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03-23-2014, 06:33 PM | #2 (permalink) |
Join Date: May 2000
Location: Old Lyme, CT USA
Posts: 21,144
orange could indicate some blood in the urine which may equate to a uti..I'd get a sample, (of the one with orange), and have it tested...
Diane Danger Danger vom Kleinen Hain aka Masi Tranquillo's Kizzy "Angel" Jakoda's Bewitchen Sami CD OA OAJ OAC NGC OJC RS-O GS-N JS-O TT HIC CGC "Angel" Steinwald's Four x Four CGC HIC TT "Angel" Harmonyhill's Hy Jynx NA NAJ NAC NJC RS-N JS-N HIC "Angel" Jakoda's Jagged Edge |
03-23-2014, 07:19 PM | #5 (permalink) |
Master Member
Join Date: Feb 2013
Location: Southern Indiana
Posts: 787
I totally agree that testing for a UTI is a good idea.
My GSD is the only animal I've ever owned that I have had to encourage to drink water, even though he always has access. It is like it is an afterthought for him. "Oh, yeah, now that you mention it, I guess I am really thirsty"! But often he is so eager to go and do things, he'll pass the bowl right up until he's reminded. So sometimes his urine has an orangish tint to it because he is somewhat dehydrated. It smells pretty strong as well. Hopefully, that is what is going on with yours as well, but it wouldn't hurt to check things out to be safe. Sent from Petguide.com Free App |
03-23-2014, 08:08 PM | #6 (permalink) |
Junior Member
Join Date: Mar 2014
Location: Okinawa Japan
Posts: 26
She is on her last day of the parasite meds she has been on it for 7 days. We are working on drinking and eating I dont' know if its the bowls but she will only eat if I put the food on my hand and I have to play with the water for her to be interested.
03-23-2014, 08:54 PM | #9 (permalink) | |
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Location: Boca Raton, Florida
Posts: 1,039
ouch, can you get the name of the drug? The doctor has to know that. Than again, if your calling the doc tomorrow you can just tell them whats going on. I would still get the name and google the side effects if possible. |
03-23-2014, 08:57 PM | #10 (permalink) |
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What kind of bowls are you using?
Misty- Samoyed Mix, Tannor- Golden Retriever CGC Robyn- GSD CGC, TC, Midnite-GSD CGC,TC, Brennan-Golden Retriever CGC, Batman-Husky/Greyhound , Apollo-GSD |
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Buchsbaum, Vicki Mabel (1968) Behavioral and Physiological Responses to Light by the Sea Anemone Anthopleura elegantissima as Related to its Algal Endosymbionts. PhD Thesis, Stanford University, 123pp.
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The endosymbiosis of algae with invertebrates may be viewed with at least two major orientations. On the one hand, one may focus on the plant and animal as essentially separate organisms living together, as the word symbiosis states. The products which are exchanged between the plant and animal and the effects of the association on either partner are then of particular interest. On the other hand, one may consider the partnership as an entity, and attempt to investigate the physiology, behavior, etc. of the symbiotic association, observing what differences may appear between the "plant-animal" and analogous non-symbiotic organisms. It is the second approach which I have tried to take in this thesis. I have concentrated on some effects of light on symbiotic and aposymbiotic sea anemones of the species Anthopleura elegantissima, particularly with respect to pigmentation and several types of behavior.
Item Type: | Thesis | ||||
Title: | Behavioral and Physiological Responses to Light by the Sea Anemone Anthopleura elegantissima as Related to its Algal Endosymbionts | ||||
Personal Creator/Author: |
Number of Pages: | 123 | ||||
Date: | August 1968 | ||||
Department: | Department of Biological Sciences | ||||
Institution: | Stanford University | ||||
Additional Information: | The dissertation contains 5 pages of color photographs. These are included in the dissertation, but those are B&W and at relatively low resolution. They were then re-scanned in color. A separate file with color jpg versions of the images is here. | ||||
Subjects: | Biology | ||||
Item ID: | 2570 | ||||
Depositing User: | Joe Wible | ||||
Date Deposited: | 18 Aug 2009 23:37 | ||||
Last Modified: | 29 Sep 2011 18:54 | ||||
URI: | http://aquaticcommons.org/id/eprint/2570 |
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Updated: 05/25/2014 11:16 PM
Created: 05/25/2014 9:46 PM WHEC.com
Fire officials say a grease fire erupted at a popular restaurant in Spencerport on Sunday afternoon.
Officials say a fire started at Slayton Place after an employee left a burner on when closing up the diner.
Firefighters say smoke was coming out of the vents and the roof, but they were able to put the fire out quickly.
No one was hurt.
News10NBC/WHEC-TV welcomes your opinion and interaction on our website, but please remember to keep it clean. If you're advertising for another business, being profane, vulgar or slanderous your post will be deleted. By commenting on this website, you agree that anything you post may be used, along with your name and profile picture, in accordance with our Privacy Policy and the license you have granted pursuant to our Terms of Use. Comments are not pre-screened before they posted.


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The surname HEMMENWAY is not included in the US Census Bureau's ranking of surnames with 100 or more people. Since fewer than 100 people with this surname were included in the 2000 Census, it is relatively uncommon.
Source: "Genealogy Data: Frequently Occurring Surnames from Census 2000", US Census Bureau.
Histopolis currently has 1 grave(s) with the surname Hemmenway.
Search the Histopols Grave Index for the surname Hemmenway.
The surname Hemmenway has a Soundex code of H550. The following 172 surname(s) may sound similar to Hemmenway since they share the same Soundex code.


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JUPITER, FL, November 28, 2012 Scientists from the Florida campus of The Scripps Research Institute (TSRI) have uncovered a group of what have been considered relatively minor regulators in the body that band together to suppress the spread of cancer from its primary site.
The discovery offers a fresh batch of possible therapeutic targets as well as new diagnostic tools with the potential to predict and inhibit the spread of cancer (metastasis) in patients suffering from the disease.
The research, published recently in The Journal of Biological Chemistry, was conducted by TSRI Professor Donald G. Phinney, a nationally recognized authority in the study of adult bone marrow-derived stem cells, and a postdoctoral fellow in his laboratory, Christopher L. Haga.
In the new study, the scientists found that a cluster of seven microRNAs (miRNA) function cooperatively to repress a process known as epithelial-to-mesenchymal transition (EMT). While EMT is part of the normal biology of cell development in some parts of the body, the process has recently been implicated in two dangerous aspects of tumor growthtumor metastasis and the growth of drug-resistant cancer stem cells.
MicroRNAs are tiny fragments of RNA found in all mammalian cells. They bind to messenger RNAs, a process that generally results in gene silencing. This cluster of miRNAs, located in a genetic region known as DLK1-DIO3, suppresses a specific signaling network in human cancers that primarily affect glands such as breast cancer.
"These results establish the DLKI-DIO3 miRNA cluster as a critical checkpoint regulating tumor growth and metastasis," said Phinney. "Our data shows that when this cluster is silenced, it accelerates tumorogenesis and proliferation by inducing EMT."
Silencing the DLK1-DIO3 genetic region is an early event for tumors, Phinney said, pointing out that micro-metastasis can be detected even in the early stages of breast ca
Contact: Eric Sauter firstname.lastname@example.com 267-337-3859 Scripps Research Institute Source:Eurekalert |


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VIEWS: 5 PAGES: 22 CATEGORY: Chemistry POSTED ON: 8/13/2010
FIELDThe field of the present invention is biological and chemical techniques for detecting nucleic acid molecules. In particular, a method for detecting nucleic acid impurities which may be part single-stranded, part double-stranded, in a mixture.BACKGROUNDTests for impurities and contaminants in biochemical products are critical in the development and validation of the purification process as well as in final product testing, where the test results provide on going assurance that the bioprocessremains under control. The consistency of the safety, potency, efficacy, and purity of an injectable product is ultimately the responsibility of the manufacturer and forms the basis of regulatory evaluation. Once a product is developed and approved, itis assumed that the appropriate bioprocess is in place to assure a consistent product.The development and manufacture of drugs and biologicals have long been regulated, particularly for normally administered agents. Recent advances in measurement and purification technology have heightened awareness of potential safety hazardsallowing for more stringent purity standards using new techniques.Therapeutic biopharmaceuticals and in vitro diagnostics made by recombinant nucleic acid and monoclonal antibody based processes are typically proteins synthesized in and harvested from cultures of genetically modified cells. Thus, the startingmaterial for the purification of each biopharmaceutical is complex, heterogeneous and potentially unsafe. Potentially dangerous impurities and contaminants include host cell molecules such as DNA. Development of appropriate analytical tools for processvalidation and quality control requires reliable assays for such impurities and contaminants.Concerns about contaminating DNA as a health risk have been around since the 1950's. The primary concern with contaminating DNA is that it may contain an oncogene, or cause an oncogene to be activated, or cause a tumor inhibitory gene to beturned off.Durin
United States Patent: 5824557 ( 1 of 1 ) United States Patent 5,824,557 Burke , et al. October 20, 1998 Method for detecting and quantitating nucleic acid impurities in biochemical preparations Abstract A homogeneous fluorescence-based nucleic acid detection and quantitation system is provided to measure nucleic acid in protein solutions. The process relies on the intercalation of a non-fluorescent dye into a double-stranded nucleic acid helix or single-stranded nucleic acid. The dye fluoresces after intercalation and the intensity is a direct measurement of the amount of nucleic acid present in the sample. Inventors: Burke; Thomas J. (Madison, WI), Bolger; Randall E. (Oregon, WI), Lenoch; Francis J. (Madison, WI) Assignee: PanVera Corporation (Madison, WI) Appl. No.: 08/626,520 Filed: April 2, 1996 Current U.S. Class: 436/94 ; 435/6 Current International Class: C12Q 1/68 (20060101); C12Q 001/68 (); G01N 033/48 () Field of Search: 436/94 435/6,810 References Cited [Referenced By] U.S. Patent Documents 4681859 July 1987 Kramer 4751190 June 1988 Chiapetta et al. 4902630 February 1990 Bennett et al. 4963658 October 1990 Kung et al. 5393657 February 1995 Letwin et al. Other References Goldman et al., Clin. Chem. 37(9), 1523-1525 (1991). . Briggs et al., Anal. Chem. 63, 850-859 (1991).. Primary Examiner: Horlick; Kenneth R. Attorney, Agent or Firm: Johnson; Mark K. Claims What is claimed is: 1. A process for detecting and quantitating an amount of a nucleic acid in a protein solution, comprising: a) measuring at least one light-emitting characteristic of a plurality of test solutions, each having a different known amount of nucleic acid and each having a nucleic acid detecting label; b) forming a curve based on the light-emitting characteristic measurements of step a); c) adding the label to a sample of the protein solution having a detergent and measuring at least one light-emitting characteristic from the sample; and, d) comparing the light-emitting characteristic measurements of step c) with the curve and determining the amount of nucleic acid in the sample. 2. The process of claim 1 wherein the label is a light-emitting compound. 3. The process of claim 2 wherein the light-emitting compound intercalates with nucleic acid and emits light more strongly when intercalating with double-stranded nucleic acid and less strongly when intercalating with single-stranded nucleic acid. 4. The process of claim 3 wherein the light-emitting compound is a fluorescence-emitting dye which is selected from the group consisting of PicoGreen.TM., acridine orange, YOYO I, PanVera Dye and ethidium bromide. 5. The process of claim 1 wherein the light-emitting characteristic is selected from the group consisting of intensity and polarization. 6. The process of claim 1 wherein the detergent is selected from the group consisting of SDS, alky sulfates, bile acids and quaternary ammonium detergents. 7. The process of claim 1 wherein the nucleic acid is selected from the group consisting of DNA and RNA. 8. The process of claim 1 further comprising detecting and quantitating an amount of a nucleic acid in a protein solution for monitoring nucleic acid removal of a recombinant protein manufacture. 9. A process for monitoring nucleic acid removal during recombinant protein manufacture, comprising: a) adding a detergent to a sample of protein solution in the presence of a purified assay buffer; b) adding a dye that strongly emits fluorescence when intercalating with double-stranded DNA; c) measuring an intensity from the dye; d) comparing the intensity from step c) with a curve for obtaining a quantity of DNA present in the sample. 10. The process of claim 9 wherein the detergent is selected from the group consisting of SDS, alky sulfates, bile acids and quaternary ammonium detergents. 11. The process of claim 1 further comprising the assay buffer having an amount of inherent fluorescence and DNA material that is negligible when obtaining the quantity of DNA present in the sample. 12. A kit for detecting and quantitating an amount of nucleic acid present in a protein sample, comprising: a) a receptacle containing a chemical label for detecting and quantitating an amount of nucleic acid present in a sample; b) a receptacle containing a purified assay buffer; c) a receptacle containing a nucleic acid standard for forming a curve; and, d) a receptacle containing a detergent for preventing nucleic acid binding to protein. 13. The kit of claim 12 further comprising a receptacle containing a known amount of double-stranded nucleic acid covalently bound to a polarizationly readable label. Description FIELD The field of the present invention is biological and chemical techniques for detecting nucleic acid molecules. In particular, a method for detecting nucleic acid impurities which may be part single-stranded, part double-stranded, in a mixture. BACKGROUND Tests for impurities and contaminants in biochemical products are critical in the development and validation of the purification process as well as in final product testing, where the test results provide on going assurance that the bioprocess remains under control. The consistency of the safety, potency, efficacy, and purity of an injectable product is ultimately the responsibility of the manufacturer and forms the basis of regulatory evaluation. Once a product is developed and approved, it is assumed that the appropriate bioprocess is in place to assure a consistent product. The development and manufacture of drugs and biologicals have long been regulated, particularly for normally administered agents. Recent advances in measurement and purification technology have heightened awareness of potential safety hazards allowing for more stringent purity standards using new techniques. Therapeutic biopharmaceuticals and in vitro diagnostics made by recombinant nucleic acid and monoclonal antibody based processes are typically proteins synthesized in and harvested from cultures of genetically modified cells. Thus, the starting material for the purification of each biopharmaceutical is complex, heterogeneous and potentially unsafe. Potentially dangerous impurities and contaminants include host cell molecules such as DNA. Development of appropriate analytical tools for process validation and quality control requires reliable assays for such impurities and contaminants. Concerns about contaminating DNA as a health risk have been around since the 1950's. The primary concern with contaminating DNA is that it may contain an oncogene, or cause an oncogene to be activated, or cause a tumor inhibitory gene to be turned off. During the 1980's, a new class of therapeutic drugs was developed and introduced to the market. These drugs are genetically recombinant human peptides or proteins and are produced by transferring human DNA to a laboratory recombinant protein expression system. These systems could be bacteria, yeast, insect cells, mammalian cell lines, or even human cell lines. The DNA that is transferred contains the genetic information which instructs the new host cell to synthesize the protein encoded by the DNA. For example, a segment of DNA could be transferred from humans to an insect cell which instructs that cell host to make the human estrogen receptor protein, or protein kinase C, or apolipoprotein E3. Before the emergence of biotechnology, it was very unusual to purify and then detect impurities in pharmaceuticals at low parts per billion levels. Today, the FDA recommends tests for detecting contaminating DNA in the final product. The FDA tends to promote analytical goals that challenge the state-of-the-art for bioanalytical measurements. Currently, the Food and Drug Administration (FDA) has established guidelines for acceptable levels of DNA in recombinant therapeutics. Each dose must contain less that 100 picograms of total DNA which is to include DNA impurities from the host cell as well as DNA contaminants which may have been introduced during the expression and purification processes. Exceptions to these guidelines are treated on a case by case basis. Pharmaceutical companies typically establish guidelines of 10 pg total DNA/dose to exceed the FDA guidelines. DNA concentration is measured both during the purification process and at final bulk release of the purified product. In the early stages of the purification process the DNA concentrations may be several mgs/ml but will be reduced greater than a million fold throughout purification. For example, a dose of 1 mg of protein, it is necessary to detect 10 pg of DNA. On a mass basis, there is 100,000,000 times more protein than DNA. (1 mg, 1 .mu.g, 1 ng, 10 pg). A problem is the measurement of trace impurities and contaminants in the presence of a relatively massive amount of product protein. DNA probe hybridization is an established methodology which is based upon annealing of labeled DNA probe to complementary sequences of contaminating DNA. Yet, traditional hybridization assays may be time consuming (e.g. 24-28 hour film development of 32P-labeled probe) and labor intensive (e.g. reagent handling and waste disposal). Hybridization assays only detect contaminating DNA which is complementary to the probe that is used. Other methods of quantitation include ultraviolet spectrophotometry techniques, protein binding methods and DNA amplification. Current methods to quantitate DNA during recombinant therapeutics manufacture include UV spectrophotometry, DNA hybridization, protein and antibody binding methods, and to a limited extent, DNA amplification. Each of these has both advantages and drawbacks in quantitating DNA. Some are rapid but lack sensitivity while others have the sensitivity but are slow and expensive. Improvements in assay performance in the areas of sensitivity, precision, dynamic range, and reproducibility have allowed for improved detection and quantitation of DNA impurities. For example, fluorescent dyes have been used to detect and quantitate DNA for decades. Many improvements have been made in the process including dyes which have higher quantum yields and therefore fluoresce more brightly. Cellular extracts typically emit more fluorescence in the ultraviolet range and less as the wavelength increases out to the red region of the spectrum. Therefore, fluorescent dyes which emit in the red spectrum tend to have less interference from naturally fluorescing cell components. Some of the newer dyes for detecting DNA only emit significant fluorescence when bound to DNA. These dyes have an advantage in that they can be added in excess to a DNA solution and not limit the sensitivity of the assay. It is known in the field of this invention that fluorescent dyes have potential utility in detecting DNA in recombinant therapeutic drugs. However, the dye alone is not sufficient to meet FDA guidelines in detecting DNA impurities. A prior art example of a DNA quantitation reagent is described in materials and information supplied by Molecular Probes, Inc., Eugene, Oreg. A detection method that had been described in the early 1900's is fluorescence polarization. Fluorescence polarization assay techniques are based on the principle that a fluorescently labeled compound will emit fluorescence when excited by plane polarized light, having a degree of polarization inversely related to its rate of rotation. If the labeled molecule remains stationary throughout the excited state it will emit light in the same polarized plane; if it rotates while excited, the light emitted is in a different plane. Specifically, when a large labeled molecule is excited by plane polarized light, the emitted light remains highly polarized because the fluorophore is constrained (by its size) from rotating between light absorption and fluorescent light emission. When a smaller molecule is excited by plane polarized light, its rotation is much faster than the large molecule and the emitted light is more depolarized. Prior art examples which utilize fluorescence polarization techniques are: U.S. Pat. No. 4,681,859 granted to Kramer, describing an immunoassay for detecting the presence of large molecular weight aqueous ligands; U.S. Pat. No. 4,751,190 granted to Chiapetta et al. and U.S. Pat. No. 4,902,630 granted to Bennett et al. describing fluorescence polarization techniques involving immunoassays with proteins. SUMMARY The present invention is a simple, sensitive and rapid nucleic acid detection and quantitation method that is capable of measuring nucleic acids under a wide range of conditions. The method is a fluorescence based assay to monitor nucleic acid removal, in process, during recombinant protein manufacture. The assay utilizes two standard curves which are optimized for the nucleic acid/dye ratios. A kit is also described which can be used to measure nucleic acid in recombinant therapeutic drugs. It consists of a fluorescent dye, a highly purified buffer, a nucleic acid standard, instructions for generating standard curves over two concentration ranges, and instructions for evaluating data generated using the kit. It may contain buffer for sample preparation, if necessary. The nucleic acid samples are diluted in a dilution buffer and the assay buffer and dye are added to the sample. Fluorescence is then measured and based on the standard curve that is generated, the concentration of nucleic acid is determined. A process for detecting and quantitating an amount of a nucleic acid in a protein solution, comprising: measuring at least one light-emitting characteristic of a plurality of test solutions, each having a different known amount of nucleic acid and each having a nucleic acid detecting label; forming a curve based on the light-emitting characteristic measurements of step a); adding the label to a sample of the protein solution and measuring at least one light-emitting characteristic from the sample; and, comparing the light-emitting characteristic measurements of step c) with the curve and determining the amount of nucleic acid in the sample. A process for monitoring nucleic acid removal during recombinant protein manufacture, comprising: adding a detergent to a sample of protein solution in the presence of a purified assay buffer; adding a dye that strongly emits fluorescence when intercalating with double-stranded DNA; measuring an intensity from the dye; comparing the intensity from step c) with a curve for obtaining a quantity of DNA present in the sample. A kit for detecting and quantitating an amount of nucleic acid present in a protein sample, comprising: a receptacle containing a chemical label for detecting and quantitating and amount of nucleic acid present in a sample; a receptacle containing a purified assay buffer; a receptacle containing a nucleic acid standard for forming a curve; and, a receptacle containing a detergent for preventing nucleic acid binding to protein. Further features and advantages of the invention will be apparent from the following detailed description when taken in conjunction with the accompanying drawings. DESCRIPTION OF THE DRAWINGS FIG. 1 illustrates a high standard curve for quantitating DNA. FIG. 2 illustrates a low standard curve for quantitating DNA. FIG. 3a illustrates the high range standard curve data analysis. FIG. 3b illustrates the low range standard curve data analysis. FIG. 3c illustrates the high range standard curve data summaries. FIG. 3d illustrates low range standard curve data summaries. FIG. 3e illustrates the statistical correlation R values for individual trials of the high standard curves, n=5. FIG. 3f illustrates the statistical correlation R values for individual trials of the low standard curves, n=5. FIG. 4 illustrates nuclease digestion of DNA standard in the presence of proteins. FIG. 5 illustrates the specificity of the present invention for various nucleic acids. FIG. 6 illustrates the effect of NaCl on fluorescent dye used in the present invention. FIG. 7 illustrates the effect of various substances on the DNA detection and quantitation method of the present invention. FIG. 8 illustrates detection of three DNA levels in three BSA concentrations using a standard spiked sample. FIG. 9 illustrates measuring the pH effect on DNA detection by using both fluorescent dye intercalation and fluorescence polarization. FIG. 10 illustrates the effect of SDS on fluorescence intensity emitted by the intercalating dye testing 3 cellular substances. DETAILED DESCRIPTION OF THE INVENTION The present invention is a method and kit for detecting and quantitating nucleic acid molecules. The term "nucleic acid", "polynucleotide", "oligonucleotide" are defined as multiple nucleotides attached in the form of a single or double stranded polynucleotide that can be natural; or derived synthetically, enzymatically, and by cloning methods. The use of the terms RNA and DNA may be used interchangeably in this description and include either double- or single-stranded nucleic acids. However, the preferred embodiments refer to any form of DNA. The method and kit include the use of a light-emitting compound which is used as a label. The present invention utilizes one or more of different light-emitting characteristics for measurement. The preferred characteristics include fluorescence and polarization techniques. In a preferred embodiment, an intercalating dye is used that binds double stranded DNA and then becomes fluorescent. Fluorescence emitting compounds include any compound having appropriate fluorescence characteristics for use with the invention. One can determine whether or not a particular fluorescence emitting compound is suitable for the present invention by comparing the candidate compound with those compounds illustrated in the Examples. If the candidate compound performs a required function such that a successful detection can be obtained, similar to the compounds used in this application, the compound is suitable for use with this invention. Potential fluorescent labels for use in the invention include, for example, fluorescent dyes: acridine orange, YOYO I and PicoGreen.TM. (Molecular Probes, Inc.), PanVera Dye (PanVera Corp., Madison, Wis.), ethidium bromide (Sigma); each used according to manufacturer's specifications, especially regarding excitation and emission wavelengths. The method is performed at room temperature, in the preferred embodiment. Reagents are warmed to room temperature. Standard Curves A curve, as used in the present invention, is at least two values obtained from measurements of light-emitting characteristics, to which a line may be plotted and used for comparison. A standard curve is generated by measuring increasing amounts of a DNA standard. The DNA standard is a known DNA, which is serially diluted into specific concentrations. A fluorescent dye is added with a light-emission characteristic which is greatly amplified when bound to double-stranded DNA. A light intensity, which in the preferred embodiment is the fluorescence intensity, value is reported at each DNA concentration. Then, a best fit line is constructed by linear regression and used to determine the DNA concentration in unknown samples. In a preferred embodiment, label nine borosilicate (PanVera Corporation, Madison, Wis.) test tubes as 1-7, 1 dye blank, and 1 buffer blank. The dye blank will contain dye solution but no DNA. It is used in the standard curve to quantitate the dye with no DNA present. The buffer blank contains no DNA or dye. It is used to blank out the background intensity in the assay buffer. 2 ml of assay buffer is added to tube 1 and 1 ml is added into all other tubes. The assay buffer is a highly purified solution containing 50 mM Tris-HCl at pH 8.0. "Highly purified" means that both the inherent DNA and fluorescence are reduced to a very low level, such that they are negligible when detecting and quantitating the amount of nucleic acid in a protein solution. The word "negligible" means the highest number one can quantitate and still meet the Limit of Detection requirements of the FDA. We obtain the buffer by, first, purchasing the most highly purified buffer that we can find with several different manufacturing lots to check. We make up a solution and check a concentrated stock such that later dilution will provide an even more pure buffer solution. We then take the buffer and add a fluorescent dye such as PicoGreen.TM. and measure the fluorescence. The specification is that we measure the background fluorescence of the buffer alone. Typically this around 1000 rfu. We then add the fluorescent dye and read the intensity. If the buffer is clean the total intensity must be less than two fold over background, which would be 2000 rfu in this case. If the buffer has DNA in it, then the intensity goes up over 2000 rfu and it does not meet specifications. The buffer should have DNA in an amount less than the lowest detection limit of the low curve, about 10 pg/ml of DNA contamination, for the lot of material to be acceptable. To remove both the inherent fluorescence and the DNA, the buffer stock is passed over an ion exchange chromatography media such as Pharmacia Q Sepharose resin (or equivalent). This resin binds negatively charged molecules such as DNA very tightly. The DNA and typically other contaminants are bound to the column and the purified buffer flows through. Some fluorescent contaminants are removed by chromatography over a cation exchange resin such as Pharmacia S Sepharose resin (or equivalent). For large molecules that contain background fluorescence, we have used membrane dialysis to allow the small fluorescent molecules to diffuse through the membrane and the large molecules were retained. We would use this method if the DNA had a lot of background fluorescence. We have used this technique to reduce the background fluorescence of BSA. Lastly, we can chemically treat protein samples to reduce fluorescence. For the BGG, we use an acetylation reaction which not only reduces fluorescence but reduces the amount of contaminating nuclease and protease activity. A suitable buffer must also have the attributes of low ionic strength along with a K.sub.a within an optimum pH range. Serial dilutions of DNA standard (20 ng/.mu.l) are made by adding 20 .mu.l of the standard to tube 1, vortexing for several seconds, then removing 1 ml from tube 1 and adding it to tube 2. Continue the serial dilution 5 more times by removing 1 ml from one tube, adding it to the next, and mixing thoroughly for a total of 7 standard tubes containing 200, 100, 50, 25, 12.5, 6.25, and 3.13 ng of DNA respectively. Discard 1 ml from tube 7. All tubes should have a volume of 1 ml. Record the fluorescence intensity value for the buffer blank for subtraction from the values obtained from each of tubes 1-7, later. Add 30 .mu.l of the dye solution to the dye blank tube and vortex for several seconds. Record the fluorescence intensity of the tube. Similarly, add 30 .mu.l of dye solution to tubes 1-7, vortex each time and measure the fluorescence intensity of each tube. Subtract the buffer blank intensity measurement from the intensity measurements of tubes 1-7. Construct a standard curve using linear regression (an example is illustrated in FIG. 1). To meet the acceptable FDA limits, we utilize 2 curves with different amounts of dye added. By doing this, we obtain FDA standard limit of detection (LOD) and limit of quantitation (LOQ) values in the picogram range that have relative standard deviation values of less than 5%. In another preferred embodiment, construct a low standard curve by making serial dilutions of the DNA standard as follows: Label eleven borosilicate tubes, 1-9, dye blank, and buffer blank. The dye blank will contain dye solution but no DNA. It is used in the standard curve to quantitate dye with no DNA present. The buffer blank contains no DNA or dye. It is used to blank out the background intensity, as described earlier. As before, dispense 2 ml buffer into tube 1 and 1 ml buffer into all other tubes. Tubes 1-9 are used to construct a standard curve. Add 2.5 .mu.l DNA standard (20 ng/.mu.l) to tube 1, vortexing for several seconds, then remove 1 ml from tube 1 and add it to tube 2. Continue the serial dilution 7 more times by removing 1 ml from one tube, adding it to the next, and mixing thoroughly for a total of 9 standard tubes containing 25, 12.5, 6.25, 3.13, 1.56, 0.781, 0.390, 0.195 and 0.098 ng of DNA respectively. Discard 1 ml from tube 9. All tubes should have a volume of 1 ml. Measure the fluorescence intensity of each tube 1-9 and record the results. Then record the fluorescence intensity value for the buffer blank for subtraction from the values obtained from each of tubes 1-9, later. Add 10 .mu.l of the dye solution to the dye blank tube and vortex for several seconds. Record the fluorescence intensity of the tube. Similarly, add 10 .mu.l of dye solution to tubes 1-9, vortex each time and measure the fluorescence intensity of each tube. Subtract the buffer blank intensity measurement from the intensity measurements of tubes 1-9. Construct a standard curve using linear regression (an example is illustrated in FIG. 2). Preparation of Test Samples The proper dilution for each sample will have to be determined empirically. A "sample" refers to any substance to be tested containing or presumed to contain nucleic acid. All samples should be diluted to a final volume of 1 ml in assay buffer. Measure the fluorescence intensity background from a diluted sample. Then, add 30 .mu.l of dye solution to the sample tube for comparison to the high standard curve, or 10 .mu.l of dye solution for comparison to the low standard curve, remembering to vortex after adding the dye solution. Measure the fluorescence intensity and subtract the background detected earlier. The subtraction step is performed automatically when using a Beacon.RTM. Analyzer from PanVera Corporation, Madison, Wis. Quantitation Use the recorded sample intensity values and convert them to ng/ml using the slope and Y intercept of the best fit line formed by linear regression performed earlier from the standard curves. For example, [Intensity in sample-Y intercept]/Slope of the best fit line=DNA concentration in ng/ml. Be sure to account for the dilution of the sample. For example, if 2 .mu.l of sample was added to 1 ml of assay buffer, there is a 500 fold dilution. Fluorescence Polarization purification can denature DNA. These include urea treatment and elevated pH. Furthermore, PicoGreen.TM. (Molecular Probes, Inc., Eugene, Oreg.), a preferred DNA intercalating dye(label), does not intercalate into single-stranded DNA very well and, therefore, does not aid in efficient detection of single-stranded DNA. According to the present invention, fluorescently labeled double-stranded oligonucleotides provide a method to determine whether conditions leave DNA in a single-strand or double-strand form. Therefore, using a combination of FP with a fluorescent oligonucleotide and PicoGreen.TM., one can determine if DNA is partly denatured. Fluorescence polarization is related to the time it takes a fluorescence labeled molecule to rotate through an angle of approximately 68.5 degrees: designated the correlation time. Correlation time is related to viscosity, absolute temperature and molecular volume. If viscosity and temperature are held constant, correlation time, and therefore, polarization, are directly proportional to the molecular volume. Changes in molecular volume may be due to molecular binding, dissociation, synthesis, degradation, or conformational changes of the fluorescence labeled molecule. Accordingly, when plane polarized light is passed through a solution containing a relatively high molecular weight fluorescence labeled compound, the degree of polarization of the emitted light will, in general, be greater than when plane polarized light is passed through a solution containing a relatively low molecular weight fluorescence labeled compound. The term "Fluorescence polarization" (P) is defined as: ______________________________________ Parallel Intensity - Perpendicular Intensity Parallel Intensity + Perpendicular Intensity ______________________________________ Parallel Intensity is the intensity of the emission light parallel to the excitation light plane and Perpendicular Intensity is the intensity of the emission light perpendicular to the excitation light plane. Since P is a ratio of light intensities, it is a dimension-less number and has a maximum value of 0.5 for fluorescein. In a preferred embodiment, fluorescence polarization (the term anisotropy is closely related to polarization and is interchangeable by a simple algebraic relationship) is used to confirm that a DNA molecule is actually double-stranded rather than single-stranded. For example, if a sample is treated at pH 11 and it reads that there are 200 ng of DNA present using PicoGreen.TM., there may be 200 ng of double-stranded DNA, or 400 ng of DNA that is 50% double-stranded and 50% that is single-stranded. Each case would indicate similar intensities because of the binding nature of the dye. However, one can use fluorescence intensity along with polarization techniques together to determine which is correct. The fluorescence polarization methods used herein are explained fully in a patent application filed before the United States Commissioner of Patents and Trademarks, entitled: Method And Kit For Detecting Nucleic Acid Cleavage Utilizing A Covalently Attached Fluorescent Tag, Assignee: PanVera Corporation, Ser. No. 08/353,079 incorporated herein by reference. In the reference, fluorescein is attached to an end of one of the strands of the double-stranded oligonucleotide. If the DNA is double-stranded, it has a high molecular weight and therefore has a high polarization value. If the DNA is denatured and becomes single-stranded, it has a lower molecular weight and the polarization value is low. If the DNA is half single-stranded and half double-stranded, then the polarization should be intermediate between the high and low polarization values of the double-stranded and single-stranded DNA. One can look not only at the two states of the DNA but can look at all the intermediate states of `strandedness` as well. This works because none of the methods used to denature DNA, such as base treatment, urea, or heat remove the covalently attached fluorescent tag from the oligonucleotide. These same treatments would very likely keep the PicoGreen.TM. from binding the DNA. It should be clearly pointed out that in the present invention there are two different fluorescent labels. In fluorescence polarization, the oligonucleotide is covalently labeled so that the fluorescent tag does not detach with the chemicals changes that we investigated. The PicoGreen.TM. (and all the other intercalating dyes) bind to DNA through electrostatic and hydrophobic forces. They are typically weaker forces and can be more easily disrupted by the chemicals used herein. To compare fluorescence polarization and an intercalating label determination (PicoGreen.TM., in the preferred embodiment), duplicate tubes are used. For the fluorescence polarization use a double-stranded covalently labeled oligonucleotide and titrate the pH over the listed range. If the DNA is coming apart, one detects a shift down in polarization. This indicates under which conditions the DNA is single or double or a mixture of both. For the other set of tubes, use Mbo I digested phi-X 174 DNA that is unlabeled. Add the PicoGreen.TM. the same range of pH samples that was used for the other DNA. As the pH is raised, one begins to observe the fluorescent signal from the PicoGreen.TM. go down. It is not known whether the diminished signal is from the DNA coming apart or whether the PicoGreen.TM. is not functional at the higher pH. The fluorescence polarization tells us that the DNA is coming apart and that is why the PicoGreen.TM. is not working. It is important to point out that in one set of tubes the fluorescence comes form a covalent tag and in the other set it is an intercalating dye. This is also a necessary test to determine if a protein purification method is denaturing the DNA. For example, the DNA could be spiked into a protein buffer containing 8M urea. This could denature the DNA so that it is not detectable by PicoGreen.TM. when it is diluted out of the urea. The fluorescence polarization method will indicate whether the DNA is single- or double-stranded and whether or not one should expect the PicoGreen.TM. to work. Detergent In another preferred embodiment, a detergent is used to enhance the quantitation method. It is known that nucleic acid has a tendency to bind to proteins. Therefore, in a protein solution that is to be tested for amounts of nucleic acid impurities, the protein could interfere with DNA measurements by competing with a dye for binding. However, the present invention provides an answer to this problem by introducing a detergent to the sample solution, which prevents DNA from binding to proteins. By interfering with the chemicals forces which bind the protein to the DNA, such as electrostatic, hydrophobic, and hydrophilic attractions. The proteins coat the outside of the DNA and prevent the PicoGreen.TM. from intercalating into the DNA double helix. In the preferred embodiment, when the samples are pretreated with SDS, an ionic detergent, the method has significantly improved performance. SDS is one example of a detergent that is useful with the present invention. However, many detergents will perform well and may be substituted for SDS in the method. A suitable detergent will be able to prevent nucleic acid binding to proteins. Particularly suitable detergents are: 1) a class called alky sulfates including LDS; 2) a class called the bile acids which includes the cholate detergents such as sodium deoxycholate; 3) a class called the quaternary ammonium class including a detergent called CTAB. If one wishes to determine whether or not a particular detergent is suitable for the present invention, one can test whether the detergent has: 1) the ability to prevent nucleic acid binding to proteins; 2) does not have inherent fluorescence that interferes with the intercalating dye fluorescence; 3) separates proteins or other contaminants from DNA and 4) does not interfere with the intercalating dye either in high concentration or when diluted out. A candidate detergent can be tested for appropriate characteristics by exposing it to a solution of proteins in the presence of nucleic acid, inserting a dye, and measuring the results. The results should be compared to results from a solution without inserting the candidate to determine its usefulness. If the candidate performs by preventing nucleic acid binding to the proteins, it is considered suitable to the present invention. Quantitation Kit A kit is formed according to the process and methods provided. In the preferred embodiment, the kit contains the following: a receptacle containing an assay buffer of 50 mM Tris-HCl, pH 8.0; a receptacle containing PanVera Dye; a receptacle containing DNA standard of 20 ng/.mu.l of .phi.X174 RF1 DNA in assay buffer. In another preferred embodiment, the items listed, plus one or more of the following: a receptacle containing SDS detergent; a receptacle containing a known amount of double-stranded DNA covalently bound to a polarization-readable label; pipettes, test tubes, test tube racks, vortexer; and, instructions for use. By the term "instructions for use," it is meant a tangible expression describing the reagent concentration for at least one assay method, parameters such as the relative amount of reagent and sample to be admixed, maintenance time periods for reagent/sample admixtures, temperature, buffer conditions and the like. Definitions from FDA's Validation of Compendial Methods (incorporated herein by reference) Precision Precision is a measure of assay reproducibility. Measure (evaluate) the variation in homogeneous samples by performing several independent analysis and determining mean and relative standard deviation. A minimum of six replicates with a relative standard deviation (RSD) and not more than (NMT) 2% is acceptable for most methods. Accuracy Accuracy measures the compatibility of the assay value with a true or absolute value or standard. For example, demonstrate the ability of the assay to reviver a know amount of analyte and express the result as a percentage. Replicates of 6 and recoveries or accuracy's of 60-100% are acceptable for concentration below 100 ppb; recoveries of 80-100% are acceptable for concentrations above 100 ppb. Limit of Detection Limits of detection specify the lowest concentration of a sample that can be detected within the method (not necessarily the lowest amount that can be quantitated). When the assay is routinely performed test samples should contain 2-3 time the minimum amount capable of detection. Limit of Quantitation Limits of quantitation specify the lowest concentration of a sample that can be quantified with an acceptable degree of precision. For instrument methods determine the standard deviation (SD) of a series of blanks, and multiply the SD by 10 for an estimate of the limit of quantitation. Confirm this with samples prepared with this concentration. Specificity Related to selectivity and interference. Specificity is a measure of the assay's sensitivity to impurities, related chemical compounds and degradation products of product excipients. They measure the degree of interference. Linearity and Range Linearity demonstrates that the method can elicit results that are mathematically related to the concentration of the analyte in the sample. Linearity is usually demonstrated over a defined range of analyte concentration. The slope of the regression line and its variance (R) provide a mathematical measure of linearity; the y intercept is a measure of potential assay bias. Range is validated by demonstrating accuracy and precision at it extremes. If the assay is not linear (log/log or log/linear) demonstrate these relationships as well. Correlation If the assay replaces an existing assay or is used to support another assay which measures a directly related characteristic, it may be appropriate to evaluate the correlation between assays. Such an evaluation requires that identical samples be tested several times by both methods. Ruggedness (Robustness) The ruggedness of an assay is a measure of its reproducibility in the face of variations such as analysts, different instrumentation, lots of reagents or elapsed assay times. To test ruggedness, compare the reproducibility of the sassy when challenged under extreme conditions with the precision of the assay under normal conditions. For example, the evaluate the ruggedness of the assay when performed by different technicians, have four analysts perform the assay per day for three days with identical samples and evaluate precision. EXAMPLES A preferred embodiment uses a proprietary dye PanVera Dye, .phi.x174 RF1 DNA from the phage phi X 174 as a standard, and Tris-HCl buffer. As the dye intercalates into the DNA helix, it emits fluorescence (530 nm) directly proportional to the amount of DNA present. This method was tested in the presence of several matrix components and proteins to see if they induce or diminish dye fluorescence. This method was compared to a spike and recovery experiment for detecting DNA spiked into protein solutions. All fluorescence measurements were performed using a Beacon.RTM. Analyzer or Beacon 2000 Analyzer(PanVera Corporation) with bandpass filters at 485 nm excitation and 530 nm emission. Fluorescence was reported in relative fluorescence units (rfu) and is dependent on the photomultiplier tube (PMT) voltage setting. The PMT voltage was kept constant for each individual experiment but may have been altered between experiments. Therefore, sample intensities can be compared within an experiment but it is not valid to compare them between experiments. This variation in PMT voltage allowed for a larger dynamic range of the instrument in measuring fluorescence intensity. It is important the DNA standard also be made up in a buffer which has very low fluorescence background. If there is light coming from the buffer containing the DNA, it will give artificially high numbers. The water that goes into making up these buffers is highly purified through 4 cartridges and then sterile filtered to remove bacteria. Bacteria can be a source of DNA contamination. The SDS that is used also has to be of low fluorescence background and low DNA content. Standard Curve Analysis The fluorescent dye solution was added to DNA in Tris-HCl buffer, mixed, and the fluorescence intensity was measured. The two blanks defined in the assay were the buffer blank (Tris-HCl buffer, 50 mM, pH 8.0) and the sample blank (buffer blank with fluorescent dye containing no DNA). The sample blank intensity was used to establish the sensitivity limits in the assay. DNA quantitation was performed using two standard curves (FIGS. 1 and 2), each optimized for dye/DNA concentration. The high standard curve was linear from 3.13 ng to 200 ng DNA with a correlation coefficient R value of 1.0000 and an LOQ (limit of quantitation) of 0.033 ng DNA. The low standard curve was linear from 0.098 ng to 25 ng DNA with a typical R value of 1.0000 and an LOQ of 0.024 ng DNA. A correlation coefficient of 1.0000 is typical for these standard curves. The LOQ was calculated by adding the mean intensity of the sample blank and 10 times the standard deviation of the sample blank. For example, for the high-standard curve, the mean blank intensity (n=5) was 2,240 rfu with a standard deviation of 42 rfu. The LOQ intensity was 2,240+(10.times.42)=2,680 rfu. On the standard curve, this extrapolated to DNA concentration of 0.43 ng/ml. Similarly, the LOD was calculated using the mean blank intensity plus three standard deviations. To demonstrate the precision in the standard curves, five low-range standard curves and five high-range standard curves were generated using the DNA method. The mean, standard deviation and relative standard deviation (RSD) from the five high-range and five low-range trials are shown in FIGS. 3a and 3b. NA Standard was serially diluted in 8 glass tubes from 150 to 1.17 ng in 1 ml of Assay Buffer. A separate dilution series was performed in Assay Buffer containing 0, 20, 40, 80, 100, 150, and 200 mM NaCl. 30 .mu.l of the fluorescent dye was added to each tube and the fluorescence intensity in each tube was measured. The graph is a plot of fluorescence intensity versus the concentration of the DNA Standard at the various NaCl concentrations. The best fit line generated using the method of least squares is also shown for each standard curve. This data shows the trend that increasing NaCl concentration decreases the sensitivity of the assay. The slope, Y-intercept, and correlation coefficient for the "best fit" line using the method of least squares are also shown in FIGS. 3c and d. The statistical correlation R values for individual trials of the high and low standard curves, n=5 are shown in FIGS. 3e and f, respectively. The RSD values for each DNA sample measurement were .ltoreq.2.0%, while the high and low sample blanks have RSD values of 2.0% and 15.7%, respectively. A standard curve was developed for single-stranded DNA using the circular phage M13mp18. Five standard curves were generated with 9 DNA concentrations from 390 pg/ml to 100 ng/ml. The relative standard deviations were less than 3.2% and the mean R value was 0.9999. Nuclease Degradation Nuclease digestion of the DNA reduced dye intercalation and therefore caused a proportional decrease in fluorescence intensity. The DNA concentration (100 ng/ml) was measured alone or in 100 .mu.g/ml bovine serum albumin (BSA) or 15 .mu.g/ml bovine gamma globulin (BGG) at 10 minutes, 1 hour, 4 hours and 72 hours. Three duplicate tubes were treated the same except that bovine deoxyribonuclease I (DNase I) and phosphodiesterase I (PDE I) were added to digest the DNA into short oligonucleotides and mononucleotides. The sample blank intensity was subtracted from each of the reported sample intensities. The fluorescence intensity in the 100 ng .phi.x174 DNA solution was reduced 99% after nuclease treatment for 72 hours (499,000-6,200 rfu) (shown in FIG. 4). The intensities of the BSA and BGG solutions were 6,200 rfu and 49,770 rfu higher than the sample blank intensity, 9,800 rfu. The fluorescence intensity due to the spiked DNA decreased more than 50% when treated with the nuclease mixture. In a separate control experiment to show that the decrease in fluorescent signal was due to deoxyribonuclease degradation and not dye/protein interactions, ribonuclease A and ribonuclease T1 were added to DNA samples. While the DNase I and PDE enzymes digested the DNA to near completion and reduced the fluorescence intensity significantly, the RNase had little effect on the DNA degradation and fluorescence intensity (less than 5% change in intensity relative to the control). Specificity Various nucleic acids were tested using the quantitation method described to determine the specificity of the assay (illustrated in FIG. 5). In these experiments, 100 ng of each nucleic acid or nucleotide sample was quantitated using the fluorescent assay and the intensity was compared to the intensity from 100 ng of the .phi.X174 standard DNA. The nucleic acids tested were of various sizes, RNA or DNA, single-stranded or double-stranded, circular, linear, or superhelical. Material was tested from bacterial, viral, and mammalian sources. Purified nucleic acids were quantitated first by ultraviolet absorbance at 260 nm. The fluorescent intensity of lambda phage, E. coli, human, and a synthetic 25 basepair(bp) oligonucleotide were 126%, 103%, and 120% respectively. .phi.X174 DNA (5386 bp) was digested with the restriction enzyme Hae III to produce 11 fragments with an average size of 490 bp and a range of 73 bp to 1353 bp. The fluorescence intensity of the 100 ng of digested DNA was 97% of the undigested control DNA. Single-stranded DNA samples ,including oligonucleotide homopolymers, were also tested for specificity in the present invention (FIG. 5). The intensity of the single-stranded oligonucleotides ranged from 0.3 to 19% of the control. An M13mp18 sample emitted at 24% of intensity for an equivalent amount of double-stranded standard. The standard curve for single stranded DNA showed that the sensitivity was lower but the linearity of assay was equal (R=0.9999). An 1,800 base synthetic RNA transcript showed <1% relative to an equal mass of control DNA. Matrix Effects on Sensitivity and Linearity The sensitivity of the DNA quantitation assay to common matrix components such as salts, detergents, buffers, solvents, and denaturants was tested. Standard curves were generated in the presence of several different concentrations of each component to determine the effect on the assay. The NaCl matrix experiment was representative of how the other experiments were performed (FIGS. 6 and 7). Seven DNA standard curves ranging from 780 pg to 50 ng were generated in the presence of 0, 20, 40, 80, 100, 150, and 200 mM NaCl. The slope of the standard curve decreased 90% with the addition of 200 mM NaCl. However, the linearity of the assay does not change (R=0.9999). The same type of experiments were performed with magnesium chloride, acetonitrile, urea, SDS, pH, NP-40, and Tris-HCl. Effect Of Various Potentially Inhibitory Compounds Standard .phi.X174 DNA was serially diluted from 25 to 0.78 ng in 1 ml volumes of Assay Buffer. The dilution series was repeated with the addition of a range of concentrations of various potentially inhibitory compounds. 10 .mu.l of the fluorescent dye was added to each tube and the fluorescence was measured on a Beacon.RTM. Analyzer. A best fit line using the method of least squares was generated for each dilution series and reported in the tables below. The slope for each dilution series containing the added compounds was compared to the slope for the dilution series containing no added compounds by reporting the slope as a percent of the control slope. The R value (correlation coefficient) is also reported for each series in order to demonstrate that although the presence of some matrix components may reduce the sensitivity of the assay, the linearity does not change significantly. The data is summarized in FIG.7. Correlation With Spike and Recovery Method The present invention was used to detect three DNA levels in three BSA concentrations (4,000 .mu.g/ml and 800 .mu.g/ml and 40 .mu.g/ml) and measured. The samples were measured directly using our method. The results are shown in FIG. 8. BSA was diluted fivefold in assay buffer of the present invention. A fluorescent dye was added to the buffered protein solution and the intensity was measured and identified as the DNA detected relative to the control (FIG. 8). The intensity could actually be due to either DNA that was an impurity or contaminant in the protein or interactions between the protein and the dye which caused it to emit fluorescence. Standard DNA (1 ng) was then spiked into each sample and the intensity was measured again. The amount of DNA recovered in the assay was determined from a standard curve and expressed as a percent of the spiked DNA in the control. The 1 ng DNA spiked into the 4000 .mu.g BSA sample represents a 250 ppb concentration. Procedures, Materials and Results Experiments were performed in which the dye was added to purified BSA or BGG spiked with standard DNA. The observed fluorescence was from the dye intercalating into the DNA helix since the nuclease digestion of the DNA to mononucleotides reduced the fluorescence greater than 95%. It is likely that during and after protein purification, DNA could be partially denatured and/or degraded. The DNA standard in the assay is a well characterized supercoiled, circular, double-stranded molecule which is 44.8% GC. We found that for long cellular and viral DNA samples, equivalent amounts of DNA emitted fluorescence values within 20% of the standard. For a short (25 bp) oligonucleotide, the fluorescence was reduced to 25% of the control DNA. The total intensity of 100 ng of the Hae III digested .phi.174 DNA was 97% of the undigested DNA showing that as long DNA fragments are shortened during purification, they are detected with very similar efficiency. The standard curve range for the fluorescence system is homogenous and takes minutes to quantitate DNA whereas other systems take hours. The present invention is designed to perform directly in protein solutions, avoiding costly sample preparation methods. Fluorescence Polarization Make a triple buffer--500 ml: 10 ml of 1M Tris; 10 ml of 1M phosphate; 100 ml of 1M carbonate, 0.5M NaCl; H.sub.2 O to 500 ml, then adjust pH to about 5.0; add buffer to 10, 50 ml beakers and adjust the pH in each to: 12.0, 11.6, 11.2, 10.8, 10.4 10.0, 9.5, 9.0, 8.5, 8.0 respectively; add 20 .mu.l of fluorescein end-labeled, double-stranded oligonucleotide to each tube and record the polarization level on a Beacon.RTM. Analyzer. PicoGreen.TM. determination of DNA: add 11 .mu.l Mbo I phi-X 174 DNA at 1.8 ng/.mu.l to 190 .mu.l of each of 10 tubes at the different pH values; Mbo I digested phiX 174 DNA to 190 .mu.l of duplicate 10 tubes containing the buffers adjusted to different pH. (Note that there are duplicates and the fluorescence from the first set comes from covalent tag and the fluorescence from the second set come from the intercalating PicoGreen.TM.) let sit for 20 minutes; add 10 .mu.l PicoGreen.TM. to 10 tubes of 1 ml buffer; add 10 .mu.l of each DNA sample and record fluorescence intensity, immediately; prepare low curve graph (shown in FIG. 9). The results show the correlation between the intensity and polarization. Plot pH value versus intensity of the PicoGreen.TM. and plot pH versus polarization value for the fluorescently tagged oligonucleotide. The polarization indicates that the DNA comes apart in the range that the PicoGreen.TM. is no longer intercalating and fluorescing. Treatment With SDS Treat a DNA/protein sample with a high concentration of SDS and then dilute out the SDS and use PicoGreen.TM. to measure the DNA. We used human recombinant estrogen receptor expressed in insect cell culture using baculovirus. We had three pools of material: whole cells, a nuclear fraction, and a cytoplasmic fraction (extracts). We used SDS and heat to determine if pretreatment gave better results. Prepare the buffer: 10 ml of 50 mM Tris-HC1, pH 8.0; ______________________________________ 10% SDS H.sub.2 O ______________________________________ Crude whole cells 1. 100 .mu.l 100 .mu.l 800 .mu.l 2. 100 .mu.l 900 .mu.l 3. 100 .mu.l 100 .mu.l 800 .mu.l 4. 100 .mu.l 900 .mu.l Cytosol 5. 100 .mu.l 100 .mu.l 800 .mu.l 6. 100 .mu.l 900 .mu.l 7. 100 .mu.l 100 .mu.l 800 .mu.l 8. 100 .mu.l 900 .mu.l Nuclear extract 9. 100 .mu.l 100 .mu.l 800 .mu.l 10. 100 .mu.l 900 .mu.l 11. 100 .mu.l 100 .mu.l 800 .mu.l 12. 100 .mu.l 900 .mu.l ______________________________________ heat tubes 1,2,5,6,9,10 to 650.degree. C., for about 20 minutes; the rest of the tubes remain at room temperature; dilute all tubes 1:100 in assay buffer 10 .mu.l to 1 ml into tubes 13-24; add 100 .mu.l of each to 900 .mu.l buffer and 30 .mu.l PicoGreen.TM. and record fluorescence intensity on high standard curve as shown previously; results are shown in FIG. 10. In an example of using fluorescence polarization to monitor detergents interrupting protein DNA interactions, we measure the polarization of the labeled double-stranded oligonucleotide. We then add the DNA binding protein and see the polarization value go up because the DNA/protein complex has a higher molecular weight. If we added SDS to this complex, it interacts with the protein and keeps it from binding to the DNA. Therefore, the polarization goes back down. This polarization experiment shows directly that we can disrupt protein/DNA binding with detergents. This is our rational for pre-treating samples with SDS. The detergent disrupts the protein DNA interactions and frees the DNA up to bind to the PicoGreen.TM.. We have to make sure that the SDS is at high enough concentration to disrupt protein/DNA binding but is then diluted out far enough so that it does not interfere significantly with the PicoGreen.TM. binding. Additional Information In a kit: for superior DNA removal it is desirable to autoclave pipettes, even disposable pipettes, eliminating significant DNA contamination. the limit of quantitation must not be greater that 0.43 ng/ml for the high standard curve. the limit of quantitation must not greater that 0.028 ng/ml for the low standard curve. the relative standard deviation values for the standard curve sample must be less than 3% to 5%. the process must detect single-stranded DNA with secondary structure by giving a standard curve with R value>0.999. the process must detect DNA in the presence of matrix components so that the linearity of the standard curve is>0.999 e.g. NaCl, acetonitrile, SDS etc. The significance of the results of potentially inhibitory substance is that even thought the LOD and LOQ are lower, the linearity (R value) is still>0.999. Sources of Materials In the experiments and methods: lyophilized bovine pancreatic DNase I, human DNA and E. coli DNA were from Sigma, St. Louis, Mo.; phosphodiesterase I from Crot. Adamanteus was from Pharmacia, Milwaukee, Wis.; .phi.X174 RFI double stranded DNA,M13mp18 single-stranded DNA, and RNA transcript, Tris-HCl, MgCl.sub.2, NaCl, and SDS were from PanVera Corporation, Madison, Wis.; Lambda DNA was from Promega Corp., Madison, Wis.; synthetic oligonucleotides were from Eppendorf, Madison, Wis.; deoxynucleotides were from TaKaRa Shuzo, Japan; NP-40 was from Pierce, Rockford, Ill.; the urea and acetonitrile were from Fisher Scientific, Fair Lawn, N.J. The foregoing is considered as illustrative only of the principles of the invention. Further, since numerous modifications and changes will readily occur to those skilled in the art, it is not desired to limit the invention to the exact construction and operation shown and described. Therefore, all suitable modifications and equivalents fall within the scope of the invention. * * * * *


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The race starts with the act of love. Tens of millions of spermatozoa rush ahead on a hunting trip that may eventually lead to a fateful encounter and a promise of new life.
Only one can win. The rest will end their lives in a matter of days. In the marathon race to life there are no consolation prizes. Only one winner will merge with the ovum.
The sperm move forward by wiggling their tails through the dangerous obstacle track, the acidity in the vagina, the cervix, and the uterus.
The obstacles claim many lives. The slow and weak give up. Others go off track, lose direction, or get trapped. The road separates into two fallopian tubes, but there is an ovum waiting in only one of them.
Not only stamina and talent count, this journey also requires a lot of luck.
Strong currents prevent the sperm from continuing forward. The follicles on the uterine wall capture many and hold them back.
Finally, at the end of the tunnel, like a mysterious star – the ovum. The sperm attack is merciless. A sperm cell carries a mysterious load: each can create a unique human being unlike any other in the world.
Now there are only about 100 sperm left competing for the ovum, but only one will make it through the ovum's protective layer. In their final efforts, one suddenly manages to penetrate. It is the winner.
After the ovum is pierced, it hardens and becomes impermeable. For the rest of the sperm, the journey has ended in failure. For the winner, it is the beginning. It sheds its now unnecessary tail and load, and is free.
The father's genetic material merges with the mother's. A new and unique cell is created: the fertilized ovum. At this moment, almost all of
the future baby's features are determined and fetal development begins.


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Cabaret Series Tackles the Lost Songs of Broadway: 1970s
* Additional fees apply. No coupon or promo codes necessary to enjoy the displayed discount price.
The last date listed for Lost Songs of Broadway: 1970s was Saturday June 1, 2013 / 9:00pm.
Currently at Signature Theatre
Cabaret: Kander & Ebb's Dark, Decadent Musical Hit
- Full Price:
- $76.00 - $99.00
- Our Price:
- $56.00 - $89.00
Step out of the modern world and back in time to the divinely decadent Kit Kat Klub of pre-war… More
Venue Details
Reviews & Ratings
Featured review from Mark Anthony Newman
Red Velvetview more less of this reviewGreat way to spend a leisurely summer evening in Arlington. Great performances and I enjoyed the fact the tunes weren't the same tired old chesnuts we hear over and over again. The direction was great, the performers outstanding, the pianist was amazing...I'm ready for the next one!
star this review starred report as inappropriateThese cabarets are always good shows -- only an hour long, but worth it -- and you can bring a drink in with you.
Quotes & Highlights
Watch the trailer for Lost Songs of Broadway.
About the Ticket Supplier: Signature Theatre
Signature Theatre is a Tony Award®-winning, non-profit professional theater company with a mission to produce contemporary musicals and plays, reinvent classic musicals, develop new work, and reach its community through engaging educational and outreach opportunities.


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Union Contract With AT&T Nears End
"Put the American back into American Telephone & Telegraph."
That was the message that Mary Lou Schaffer, President of Communication Workers of America (CWA) Local 13550, along with about 100 members and supporters, were saying as they protested outside the AT&T building in downtown Pittsburgh this morning.
The existing labor contract for 40,000 employees nationwide, including 250 at Legacy T Core call centers in Pittsburgh, expires at midnight April 7.
According to Schaffer, the main stumbling block in contract negotiations is AT&T's outsourcing of jobs to places such as Slovakia, Mexico, India, and the Philippines.
"You know, at one time we had over one thousand people in this building, not too long ago — and now we're down to eighty," Schaffer said.
The negotiations have been ongoing since February and, according to the union, little progress has been made. Schaffer says they want to continue to bargain, but it appears the company wants to "squeeze out" more profits.
"For a company such as AT&T, who made over $20 billion in profit over the last 2 years, you would expect them to step up and provide good jobs to change the economic recovery and move the country forward; and part of that would be keeping jobs in America," Schaffer said.
Schaffer said union members across the country have overwhelmingly authorized a strike, but no walkout is planned at this time.
AT&T did not returns requests for comment.


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Model #670330 UL Approved Security Drawer
Shuresafe's Model #670330 is a UL approved Level 3 bullet resistant stainless steel security drawer for transferring items to and from the operator without opening doors. This unit features a built-in speaker, which attaches to an existing intercom system. Model #992217 is an extended version of Model #670330 that is designed for installation in up to 8-inch walls.


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CONTENTSFind us on iTunes!Main Website:SPORTSBaseballBoys' BasketballGirls' BasketballCross CountryFootballBoys' GolfGirls' GolfBoys' SoccerGirls' SoccerGirls' SoftballBoys' TennisGirls' TennisTrack & FieldGirls' VolleyballWrestlingOTHERMEDIAHeadlinesLive BroadcastsArchived BroadcastsVideo ClipsPhoto GalleriesAnnouncementsCalendar/ScheduleiCal CalendarTournamentsContent PagesDocumentsForumsPollsScoreboardContact UsRSS FeedIHIGH LINKS |
Home » Football NewsLouisville Vs. Rutgers, This Years Big East Impact GameJuly 4, 2012 By Jon Hancock of KentuckySports.co According to ESPN's Andrea Adelson, the Louisville vs. Rutgers game in November 29th will be the biggest impact game for the Big East conference. For the complete article go HERE. Excpert: Given what is returning to both teams, it is easy to see why this game should have a major impact. Louisville has 15 starters returning, including up-and-coming quarterback Teddy Bridgewater. There is depth at running back and receiver; the offensive line should be markedly improved. Defensively, Louisville has one of the best secondaries returning, along with two of its starting linebackers.
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Overcome The Barrier (OCTB) is a U.S.-based language training company. Since 2008, we have provided language training to companies and individuals around the globe. Our dedication to service, transparency and objectivity set us apart from our competitors.
Skype and Google Hangouts have become global leaders in videoconferencing. Both services offer fast, secure and stable platforms for effective videoconferencing. Once you are logged on, your instructor will initiate a video call with you at the scheduled time.
OCTB instructors are trained to conduct the lessons according to your needs. For example, if you are a computer programmer, an instructor may propose discussions on big data, computer security, and other technology-related topics. Additionally, you may discuss other topics that interest you. The main objective of this conversation is for you to use the target language in a practical way.
During the conversation segments of the lessons, your instructor will transcribe everything you say in a Google Spreadsheet.
The corrections allow you to see your mistakes and thus learn to avoid them in the future. If you internalize these corrections, you will make progress much faster than you would using traditional language learning methods.
The OCTB method is effective for two reasons. Firstly, the OCTB method creates an authentic speaking context for all language learners. In other words, you will begin using the language you want to learn from the first day, regardless of your level. By creating a real context in which you can use the target language, the OCTB method simulates real life situations that you would experience if you lived in an area where the target language is spoken.
Secondly, as you speak our instructors type every word you say, and following the conversation they go through every sentence and make corrections to your speech. As they correct your grammar mistakes, you not only hear the corrections, but you also see the corrections in real time.
The corrections process targets both visual and auditory learners. According to one study, 65 percent of people are visual learners, and additional research shows that both visual and auditory learning styles are dominant. These figures are important because they explain why individuals who study with OCTB make progress so much faster than those who study for years using traditional formats of instruction.


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This meta category should only contain other categories. Files should either be in the relevant subcategory or in the parent category. |
English: This is a category of airports by name. All categories about specific airports or airfields (civil or military, current or defunct) should be included as direct subcategories.


text:
Schools in Abingdon, VA
About Abingdon Schools
- There are 9 K-12 schools in Abingdon, VA, including 8 public schools and 1 private schools. Abingdon public schools belong to two districts, A. Linwood Holton Gov School District and Washington County Public Schools School District.
- There are 4 Abingdon elementary schools, 2 Abingdon middle schools, 4 Abingdon high schools and 13 Abingdon preschool schools.
Sponsored Link
Abingdon School Districts
- A. Linwood Holton Gov School (1 school)
- Washington County Public Schools (17 schools)
Contact Education.com with questions or feedback about SchoolFinder.
Please note, if you wish to speak to someone at the school, you must contact the school directly.


text:
Package: libembryo0 (0.9.9.061-2)
Links for libembryo0
Debian Resources:
Download Source Package embryo:
- Debian Pkg-e Team (QA Page, Mail Archive)
- Albin Tonnerre (QA Page)
- Jan Lübbe (QA Page)
- Xavier Oswald (QA Page)
External Resources:
- Homepage [www.enlightenment.org]
Similar packages:
SMALL-based abstract machine (AMX) bytecode interpreter
Embryo is primarily a shared library that gives you an API to load and control interpreted programs compiled into an abstract machine bytecode that it understands. This abstract (or virtual) machine is similar to a real machine with a CPU, but it is emulated in software. The architecture is simple and is the same as the abstract machine (AMX) in the SMALL language as it is based on exactly the same code. Embryo has modified the code for the AMX extensively and has made it smaller and more portable. It is VERY small. The total size of the virtual machine code AND header files is less than 2500 lines of code. It includes the floating point library support by default as well. This makes it one of the smallest interpreters around, and thus makes is very efficient to use in code.
See also http://www.compuphase.com/small.htm for details on SMALL.
Other Packages Related to libembryo0
- dep: libc0.1 (>= 2.3)
- Embedded GNU C Library: Shared libraries
also a virtual package provided by libc0.1-udeb
- sug: libembryo-bin
- SMALL compiler creating Embryo bytecode
Download libembryo0
Architecture | Package Size | Installed Size | Files |
kfreebsd-i386 | 19.5 kB | 60.0 kB | [list of files] |


text: Apple closes at record high $276.57 by Philip Elmer-DeWitt @FortuneMagazine September 16, 2010, 8:21 PM EDT E-mail Tweet Facebook Google Plus Linkedin Share icons Climbs $6.35 for the day, passing a record $274.07 set last June With the iPad set to go on sale the next morning in China and five South American countries, Apple AAPL shares rose $6.35 (2.35%) Thursday to close at a record $276.57. The stock is still a couple dollars shy of the record intraday high of $279.01 set on June 21, 2010. [Follow Philip Elmer-DeWitt on Twitter @philiped]


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The Renaissance men
Renaissance Technologies is a hedge fund firm headquartered in Long Island, N.Y., with additional offices in New York City and London. The firm, which uses complex mathematical theories and statistical methods in its investment decisions and strategies, has approximately $15 billion in assets under management and employs 275 people. Renaissance was founded in 1982 by James Simons, a former Cold War code breaker and MIT and Harvard math professor who now serves as the firm's non-executive chairman (he retired as chairman of the firm in 2010). Renaissance is currently led by co-CEOs Peter Brown and Robert Mercer -- who previously worked together at IBM and even shared an apartment in New York City when they started at IBM. Brown has a PhD in computer science from Carnegie Mellon University, and Mercer has a PhD in computer science from the University of Illinois. According to The Wall Street Journal, Brown is politically liberal and has been known to ride a unicycle around the office, while Mercer is politically conservative and keeps a model toy train in his basement.
600 Route 25A33rd Floor East Setauket, NY 11733 www.renfund.com
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We asked for your photos of the 2014 Bank of America Chicago Marathon and here... See Full Gallery »
A numerical roundup of what makes the Bank of America Chicago Marathon so specia... See Full Gallery »
You've trained hard all year and fulfilled your goal of running the 2014 Bank of... See Full Gallery »
The 2013 Bank of America Chicago Marathon generated more than $253 million worth of economic activity for the area, race officials announced Thursday. Read »
Can you beat these celebrities' Chicago Marathon times? See Full Gallery »
With only 45,000 spots available, marathon lottery applicants may need a back-up plan to get into the race. Read »


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English and Scientific names: |
Eurasian Wigeon (Anas penelope)
Number of individuals: |
1 adult male, alternate plumage |
Locality: |
Lafourche |
Specific Locality: |
Port Fourchon area:
large pond/impoundment north of |
Date(s) when observed: |
9 January 1990 NOTE: I submitted details on this
sighting to the LBRC in early 1990 but did not have a long form to record
them on [I was then in graduate school in Austin, TX and only returning to
La. on occasional weekends]. Because of the absence of the official
form, I assume, the record was not reviewed. See attached file for
copies of those records, acknowledgement of their receipt by LBRC Secretary,
and 2 photos. I am grateful to the LBRC for their belated review of my
report now. |
Time(s) of day when observed: |
Approximately 11:15 am until 11:55 am |
Reporting observer and address: |
Gay Gomez |
Other observers
accompanying reporter who also identified the bird(s): |
(My father, Russell Gomez, also saw the bird
but did not identify it; he is not a birder.)None on the day of my initial
sighting, but see below. |
Other observers who
independently identified the bird(s) |
OTE: After returning to the |
Light conditions (position
of bird in relation to shade and to direction and amount of light): |
The late morning light shining on the bird was
strong, for the sun on this clear, cool, early January day was behind me as I
looked north toward the pond. Temperatures were in the mid-60s, so heat
waves were minimal. |
Optical equipment: |
Bushnell 7 to 21 x 40 zoom binoculars, 21
power used, condition of binoculars excellent |
Distance to bird(s): |
Approximately 200 meters |
Duration of observation: |
40 minutes |
Habitat: |
Open water of large pond/impoundment with
marshy edges, shallow enough for dabbling ducks to feed |
Behavior of bird: |
The bird was swimming slowly amid a large
flock of American Wigeon. It occasionally
tipped to feed (dabbled: tail up, head under the water), just as did the
American Wigeon. The bird moved around during
the time I observed it (approx. 40 minutes) so that I was able to view it
from several angles. |
Description: |
Additional details of these observations are
recorded below in the "Description" section and are taken directly
from my field notes. |
Voice: |
Not noted |
Similar species: |
The bird was not a Redhead, for it had a pale
peach forehead and crown identical in extent (though not in exact color) to
that of the American Wigeon with which it was
swimming and feeding. The white flank patch and light breast of the
bird I observed were also not characteristic of Redhead plumage. The
bird also fed in the same manner as the American Wigeon:
by dabbling, not diving or completely submerging. |
Photographs or tape
recordings obtained? |
2 photographs are attached. They are
scanned from 2 slides taken by Gwen Smalley on 14 January 1990 and sent to
Gay Gomez shortly thereafter, for documentation purposes; I am grateful to
Gwen for her thoughtfulness. (View the slides in full-screen/slide show
mode, and you'll see the Eurasian Wigeon near the
center foreground in each.) |
Previous experience with
this species: |
This was my first sighting of a Eurasian Wigeon. |
Identification aids: |
I used the National Geographic Field Guide to
the Birds of North America during and after the observation. |
This description is written from: |
The description is taken from notes made
during and immediately after the observation. I have quoted from the
notes in the "Description" section above and have extracted and
paraphrased some of the information to conform to the questions and format of
this form. |
Are you positive of your
identification? If not, explain: |
Yes, I am positive of my identification. |
Date and time: |
25 March 2010, 4:00 pm |


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Chris sent in his homemade controller for an old APC power distribution unit. This is a great way to recycle some old electronics.
I found some 'flaky' APC AP9217 PDUs in the trash and grabbed a few to play with. These have 8 power plugs that can be individually toggled which is infinitely useful for cycling the power on anything. As well as documenting how I modified this device this page is meant to serve as a general hardware hacking HOW-TO since it is a pretty easy to follow example. [via]
DIY Power distribution - [Link]
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text:
Search Result
Search Tags: National Bike to Work Week
Bicycling being embraced in Hagerstown, Md.
The western Maryland city of Hagerstown is embracing bicycling in a big way.
Tags: Antietam Velo Club , Tour of Washington County , Barbara Ingram School for the Arts , Hagerstown
Tuesday - 05/17/2011, 09:40am EDT


text:
Did you know?
- Over half of Internet users get at least 1 phishing email per day.
- Over 100 billion spam emails are sent each day (as of 2011).
- According to Consumer Reports, the cost of phishing is nearly $500 million per year in the United States alone.
- What is Phishing? "The process where a targeted individual is contacted by email or telephone by someone posing as a legitimate institution to lure the individual into providing sensitive information such as banking information, credit card details, and passwords..."
- Phishing and Identity Theft: "You may think that a person can't do a whole lot with your bank username and password, but you would be wrong. In reality, it is a very slippery slope. All too often, that slope culminates in full-blown identity theft..."
- How to Prevent Phishing: "A lot of phishing emails claim to come from legitimate sources or popular websites. The emails often ask the user to enter bank details or other personal information. There are also phishing scam websites which appear exactly like the original websites..."
- Other Resources: History of Phishing, Report Phishing, Phishing Techniques, Phishing Scams, Anti-Phishing Software.


text:
Media Home | Contact Us |
PRESS CONTACT: Sonja Olsen, Ph.D. CDC, National Center for Infectious Diseases (404) 639–2206 |
Group A rotavirus, which is the most common cause of diarrhea in children, can also cause outbreaks of gastroenteritis in healthy adults.
PRESS CONTACT: Dixie Griffin CDC, National Center for Infectious Diseases (404) 639–3628 |
Childhood lead poisoning in young children still remains a problem in some local areas.
PRESS CONTACT: Sharunda Buchanan, M.S., Ph.D. CDC, National Center for Environmental Health (404) 639–1781 |
CDC Home | Search | Health Topics A-Z This page last reviewed Friday, December 22, 2000 Centers for Disease Control and Prevention |


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Today In Music News: Weezy Has New Album, Hard L.A. Fest Gets Canceled And Jay-Z Drops A Mixtape
Categories: DFW Music News
Your daily dose of national music news for Tuesday, July 13, 2010...
- Despite being locked up, Weezy is dropping an EP this September. I guess it's a good thing to have tons of material recorded and stocked away in case of the occasional prison sentence...
- The Hard L.A. electronic music festival has been canceled due to security concerns.
- Jay-Z knows how to give fans a deal: He and DJ Green Lantern released the Creative Control mixtape for only $2.99.
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