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This original artwork by San Francisco-based artist and designer John Beck McConnico was created to support communities in crisis. 50% of the proceeds from this sale will be donated to the American Red Cross, going towards worthy purposes such as providing meals, water containers, and emergency shelter for victims of disaster. Made with mixed media on an aluminum and wood panel, this striking piece is a tangible acknowledgment of your generosity.
Unframed. Signed and dated by the artist.
90 Days of Art is an online art journal and exhibition for charity. To create the series, artist John Beck McConnico makes an average of one artwork per day for a 90-day period. Each piece captures a precise moment, specific geographical location, and state of being. The unifying theme across all works is the manipulation of context and perception.
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Overeem is injured but Hunt has said he is down for replacing him and is now rallying his twitter followers to call for the fight with dos Santos.
Overeem is injured but Hunt has said he is down for replacing him and is now rallying his twitter followers to call for the fight with dos Santos.
3-2-0 Pro Fighter managed by TFL Sports Agency
BOOM!!!... War Hunt... I think Hunt could 3 round punch smash JDS.
if JDS doesn't have the sniffles this time, he could be ok. but beware of the sniffles bro or you get squash
'Go read a fucking book, you piece of shit.'
Love the fairytale idea of Hunt going on a decent run guess he has earned his stripes over the years would love to see him blast dos Santos and possibly earn a chance to fight Cain odds are against him but we have seen stranger things happen.
If Hunt vs Nelson happens, I never thought I'd say this but Nelson would get laid out.
I can't see any UFC heavyweight beating him on the feet, his chin is crazy and his hands are heavy as fuck.
As much as I hate to say it I think JDS would have hunt on the feet, he's very mobile for a big guy and must have a large reach advantage.
Hunt vs Overeem is the fight I want to see more than any other HW matchup. I wouldn't be suprised to see Overeem take it to the ground pretty damn quickly and he certainly won't be so fckin arrogant as in his last fight.
Nelson should beat Hunt but he will stand and trade as that is the sort of fighter he is, to his detriment. It's gonna be a great fight
Looks like for the second time in as many years #RallyForMarkHunt proves pointless as Dana says the Overeem vs. JDS fight has been moved to the summer.
3-2-0 Pro Fighter managed by TFL Sports Agency

subset: commoncrawl


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Seizing on their personal bond, President Barack Obama and Indian Prime Minister Narendra Modi said Sunday they had made progress on nuclear cooperation and climate change, with Obama declaring a "breakthrough understanding" in efforts to free U.S. investment in nuclear energy development in India.
Supporters of Egypt's ousted Islamist President fought street battles with police in Cairo and other cities on Sunday, the fourth anniversary of the country's 2011 uprising, as clashes left at least 13 people dead and dozens injured. Another two...
Radio and television host Joe Franklin died Saturday of prostate cancer,
It's safe to say that Birdman is officially the frontrunner to win Best Picture at the Oscars next...
Blake Shelton pulled double duty on
This article warms my heart to no end. I don't care that it is merely a blog entry and...
Aging athletes, new skis and a lack of snow are ganging up on the once-edgy sport of snowboarding, which has seen a marked drop in participation over the last decade.
Law enforcement officials found no bombs on two planes at Atlanta's main airport after authorities received what they considered credible threats, FBI spokesman Stephen Emmett said.
Saying his team "followed every rule to the letter," New England Patriots coach Bill Belichick described in detail how his team prepares its footballs on game day and defended his players from chatter that they made it to the Super Bowl by cheating.
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Dakota Jay Yoder, age 10, of Centreville, died Thursday, Dec. 18, 2014, at Bronson Methodist Hospital in Kalamazoo, from injuries received in a four-wheeler accident.
At least these Sony hacks were good for business for someone.�On Monday, USA Network ordered to series�Mr.
When NBC's gruesome psychological horror�Hannibal�returns for Season 3, it'll bring with it a new...
Sturges-Young Auditorium in Sturgis is accepting orders for "reheat and eat" holiday dinners and pie.
Hillary Clinton's circle wants to be quoted yawning at Jeb Bush, a sure sign of concern that he could beat her in 2016.
There are considerations for everyone involved in choosing flooring. Color, style, traffic type, room usage, durability and cost are a few of those considerations. Another is creativity.
Original Post Located at:A Voice Of Reason - by Sherry Petro-SurdelOrginal Author:
The holidays are in full swing, which means there are plenty of parades, marathons, Christmas movies and New Year's Eve specials to check out over the next two weeks. Here's our handy list for what's planned as 2014 comes to an end. All times...
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IP Moulding, a manufacturer of prefinished mouldings for the Building Products Industry, is looking for a 1st shift supervisor to lead the finishing and packaging departments. The position requires a minimum of three years supervisory experience, people handling skills, analytical skills and manufacturing process knowledge.
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IP Moulding
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45W. 750N. Howe, IN
No phone calls please!
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Dexter Axleâ�s Ventline/Dexter Door plant in Bristol, IN, has immediate openings for Experienced Production Workers in a drug free, incentive based work environment.
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902 South Division Street
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Direct Care Staff working with people with disabilities in a sheltered workshop or residential setting. Benefits available.
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Or send resume to firstname.lastname@example.com
Everlast Roofing Inc, a manufacturer of steel roofing products located in Howe, IN is seeking full time class A CDL licensed Truck Drivers.
We have new equipment to Drive and Pull, and all product is no touch freight. (Loaded and unloaded with forklift) Home every weekend.
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send resume to:
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Everlast Roofing Inc
10 Enterprise Court
Lebanon, Pa 17042
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Truck Driver Opening, Full Time Position
Qualified Person should possess:
�CDL Class A
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Downtown, big swimming pool , Free Cont/ Breakfast, Free Internet, Wireless, Free Salsa lessons, Live Music (Guitar),... More Info »
The best value in Merida....great price and a great place...relax,tour around,check out this great city,rest and... More Info »
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The House of Quetzal, even though it was completely renovated, while maintaining the old style typical of colonial... More Info »
Centrally located in the heart of Merida`s historical center, El Jardin is located just 3 short blocks from the main... More Info »
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Windsurfing in FloridaLocated on beautiful St Pete Beach, in Tampa Bay near St. Petersburg, Tampa or Clearwater on the west coast of Florida, we emphasize a fun and safe windsurfing lessons or stand-up-paddle board rentals that will leave you smiling. Please call ahead 727.656.6569 for lessons and rentals.
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In the Spring, we have 3 day Beginning Windsurfing Clinics as well as the Sail Easy Intermediate Windsurfing Clinics Our US Sailing certified Windsurfing Instructors are trained to provide you with top-quality teaching methods and techniques in your Windsurfing Lesson, so you will enjoy it with a smile. Our new Shop address is 4227 Gulf Blvd Important note! Generally we will meet you at the sailing area on the directions page Please call ahead for lessons and rentals: Britt email@example.com come and enjoy Windsurfing with us in the warm shallow water of Tampa Bay, Florida. Teaching Windsurfing and getting people involved in Windsurfing is not part of what we do, it is what we do. We like to think that we do it well. |


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TS1 - phil&teds bebe hs & bebe*
No need to disturb your baby - this car seat adaptor lets you click & cruise. Move your car seat from car to stroller & back again!
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Top Definition
To show off one skills.
Spencer: im a comp sci and social psych major
Chris: well there's no need to brag about it jesus.
Chris: well there's no need to brag about it jesus.
di Chris Gwynne
28 febbraio 2006
6 more definitions
A white girl who has sex with a black man.
"Man, you don't want to get with that bitch after she fucked LaRon, she's a b-rag."
di mrbiggieduet22
05 gennaio 2009
This is a type of tool found only in the town of alpine. They are egotistical ass wipes who spend their time working out, going to church, and telling kids they're "gay". They don't do well in school and the only friends they have, are people who feel bad for them.
Sentence: Why does he have to be so rude about everything, he's acting like a Brag.
di kw1195
24 ottobre 2010
Refers to a small looking bag, that is capeable of holding large objects. Seems to be magic, but it is a bottomless bag.
Can hold many useful objects.
eg- an umbrella + cigarettes + purse + mp3+ make-up + airsoft gun etc....
Can hold many useful objects.
eg- an umbrella + cigarettes + purse + mp3+ make-up + airsoft gun etc....
di YvonneTea
07 febbraio 2010
Verb: to brag is to drink alcohol. Noun: a brag is a drink containing alcohol.
"Let's go out a brag the night away."
"Hey, you want a brag?"
"Hey, you want a brag?"
di ykdrummer91
17 aprile 2010
someone you think to be a real "friend" but will do anything they can to ruin your chances with a lady. a person who cock blocks and hates for no reason, also has deep mental problems. in addition too being very two faced.
i had a thing going with this girl I liked but fucking B-Rag cock blocked me with some lies.
di Cock blocked by B-Rag
02 febbraio 2010
To brag (shag) a person or persons
"I would brag her hard"
"Someones about to get bragged"
"Someones about to get bragged"
di King Ell
27 dicembre 2005


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Vehicle Input
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Airaid Stock Replacement Air FiltersWe manufacture direct replacement filters. Our direct replacement filters have a feature that makes them the most durable in the industry-STAINLESS STEEL. Combine our 304 S.S. mesh with our five layer media, and you have the finest filter in the world! Using durable media and premium urethane to bring you superior filtration, it is no wonder we can offer a lifetime "No Hassle Warranty". We know that our filters will last the life of your vehicle. |


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Emphasizing durability and style, the Sidi Spider Carbon SRS Techno is the perfect choice for high-performance, high mileage cross-country riders or cyclocross racers. Breathable Technomicro upper keeps your feet comfortable, while an integrated rubber toe guard provides a new level of protection and durability. Spider's unique Sole Replacement System (SRS) features replaceable rubber tread, bolted to the shoe's new durable carbon composite sole, which is 40 grams lighter than the previous Dragon sole. The patented system allows easy replacement of worn-out tread, increasing the life of the shoe. The design also provides a slight amount of flex in the toe while retaining excellent stiffness and power transfer. The 2-way caliper buckle offers secure, easy on-bike adjustment with incremental tightening and loosening for the perfect fit. An adjustable soft instep closure system and padded tongue eliminates pressure points for lasting comfort, while the high security Velcro straps provide a secured position on the foot. Standard width.
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While your female companion is on her period, one should pull out just prior to 'Nutting" (See definition for nutting) and 'cum' (See definition for cum) in said companion's mouth.
Aw yeah puta you ready fo dis blood shot biyitch!!!!!
by tastywaves
June 16, 2009
2 more definitions
Top Definition
Veins being visible in the eyes. It's a common side affect of marijuana use.
"Dude, your eyes are super-bloodshot. You had any weed recently?"
by Synthme
May 09, 2010
A bloody ejaculation or cumshot, typically caused by trauma to the testicles such as torsion, surgery, or lighting your crotch on fire an then having someone stomp it out.
Dude, I had a vasectomy three days ago and I'm still putting bloodshot on my wife's face. The clots are kinda gross.
by It really happened to me
August 02, 2010


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Custom Classic Trucks Magazine Tours the Starbird Hall of Fame Museum
Written on January 14, 2004 You're Not Going to Believe This Place!If you have lots of gray hair, before it turns all white, we suggest you see Darryl Starbird's National Rod & Custom Hall of Fame Museum. Have trouble remembering what dinner was last night? You'll very likely remember every famous vehicle in this amazing collection. What ever happened to the wild and crazy, goofy, zany, insane show cars from the '50s and '60s? Most of those show cars never ran, but we remember them anyway, and they now reside in the Starbird Museum.
We recently visited show car heaven, where many of these creations survive and are on display -- a good number restored to their original condition. Some have even evolved to never-before-seen versions. For many of us who have stacks of old car magazines in storage, you'll see these featured vehicles are on the cover of those '50s and '60s periodicals - hundreds of which you'll see hanging on the Starbird Museum's walls.
Darryl Starbird is singularly responsible for this wonderful attraction and could arguably be considered the father of the scratch-built, wild-'n'-wooly show car, especially considering the fact that he built a large percentage of the vehicles in his museum. Well-known for his bubble-topped creations, and later for his premium quality indoor shows, the man has never been far from the world of custom cars. He even has several currently in-process in his shop, and, yes, one of them has a bubble top.
Starbird is one handy man, building the museum, his shop, and his home on his Afton, Oklahoma, estate.
Space doesn't allow one magazine to cover even a small percentage of the Starbird saga, much less describe his incredible museum. Since CCT is a truck magazine, we'll show you a few of the trucks -- well, sorta trucks -- you may visit. Reason figures that if you love old trucks, you'll also love old show cars. Next time you're on the road in Oklahoma, schedule a stop at the museum. It's well worth the drive, and it's quite a trip down memory lane. Be sure to check the website for current hours.


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counter culture. All-in-one dining table, cocktail bar, storage cabinet, work space by Mark Daniel of Slate Design is white-hot with legroom and legions of stash space. Sleek, hi-gloss white lacquer unit free-floats or goes up against the wall with counter-height seating for three: one each side, plus one more at recessed end. Two-sided storage at one end stocks bottles, barware, gadgets, dinnerware, flatware, table linens, cookbooks and magazines. Center cubby has hidden cord management for work or play: charge laptop, phone or tablet; dock MP3 player to spin party playlists. Learn about Mark Daniel on our blog.


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MATLAB and Simulink resources for Arduino, LEGO, and Raspberry Pi
Learn moreOpportunities for recent engineering grads.
Apply TodayNew to MATLAB?
This solution is locked. To view this solution, you need to provide a solution of the same size or smaller.
%% x = [1 2; 3 4]; y_correct = [3 1; 4 2]; assert(isequal(RotateMatrix(x),y_correct))
Error: Assertion failed.


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Summary: Zinc-binding RING-finger
Pfam includes annotations and additional family information from a range of different sources. These sources can be accessed via the tabs below.
This is the Wikipedia entry entitled "Zinc finger". More...
Zinc finger Edit Wikipedia article
A zinc finger is a small protein structural motif that is characterized by the coordination of one or more zinc ions in order to stabilize the fold. Originally coined to describe the finger-like appearance of a hypothesized structure from Xenopus laevis transcription factor IIIA, the zinc finger name has now come to encompass a wide variety of differing protein structures.[1]
Proteins that contain zinc fingers (zinc finger proteins) are classified into several different structural families. Unlike many other clearly defined supersecondary structures such as Greek keys or β hairpins, there are a number of unique types of zinc fingers, each with a unique three-dimensional architecture. A particular zinc finger protein's class is determined by this three-dimensional structure, but it can also be recognized based on the primary structure of the protein or the identity of the ligands coordinating the zinc ion. In spite of the large variety of these proteins, however, the vast majority typically function as interaction modules that bind DNA, RNA, proteins, or other small, useful molecules, and variations in structure serve primarily to alter the binding specificity of a particular protein.
Since their original discovery and the elucidation of their structure, these interaction modules have proven ubiquitous in the biological world. In addition, zinc fingers have become extremely useful in various therapeutic and research capacities. Engineering zinc fingers to have an affinity for a specific sequence is an area of active research, and zinc finger nucleases and zinc finger transcription factors are two of the most important applications of this to be realized to date.
Zinc fingers were first identified in a study of transcription in the African clawed frog, Xenopus laevis. A study of the transcription of a particular RNA sequence revealed that the binding strength of a small transcription factor (transcription factor IIIA; TFIIIA) was due to the presence of zinc-coordinating finger-like structures.[2] Amino acid sequencing of TFIIIA revealed nine tandem sequences of 30 amino acids, including two invariant pairs of cysteine and histidine residues. Extended x-ray absorption fine structure confirmed the identity of the zinc ligands: two cysteines and two histidines.[3] The DNA-binding loop formed by the coordination of these ligands by zinc were thought to resemble fingers, hence the name.[1] More recent work in the characterization of proteins in various organisms has revealed the importance of zinc ions in polypeptide stabilization.[4][5]
Zinc finger (Znf) domains are relatively small protein motifs that contain multiple finger-like protrusions that make tandem contacts with their target molecule. Some of these domains bind zinc, but many do not, instead binding other metals such as iron, or no metal at all. For example, some family members form salt bridges to stabilise the finger-like folds. They were first identified as a DNA-binding motif in transcription factor TFIIIA from Xenopus laevis (African clawed frog), however they are now recognised to bind DNA, RNA, protein, and/or lipid substrates.[6][7][8][9][10] Their binding properties depend on the amino acid sequence of the finger domains and on the linker between fingers, as well as on the higher-order structures and the number of fingers. Znf domains are often found in clusters, where fingers can have different binding specificities. There are many superfamilies of Znf motifs, varying in both sequence and structure. They display considerable versatility in binding modes, even between members of the same class (e.g., some bind DNA, others protein), suggesting that Znf motifs are stable scaffolds that have evolved specialised functions. For example, Znf-containing proteins function in gene transcription, translation, mRNA trafficking, cytoskeleton organization, epithelial development, cell adhesion, protein folding, chromatin remodeling, and zinc sensing, to name but a few.[11] Zinc-binding motifs are stable structures, and they rarely undergo conformational changes upon binding their target.
Initially, the term zinc finger was used solely to describe DNA-binding motif found in Xenopus laevis, however it is now used to refer to any number of structures related by their coordination of a zinc ion. In general, zinc fingers coordinate zinc ions with a combination of cysteine and histidine residues. Originally, the number and order of these residues was used to classify different types of zinc fingers ( e.g., Cys2His2, Cys4, and Cys6). More recently, a more systematic method has been used to classify zinc finger proteins instead. This method classifies zinc finger proteins into "fold groups" based on the overall shape of the protein backbone in the folded domain. The most common "fold groups" of zinc fingers are the Cys2His2-like (the "classic zinc finger"), treble clef, and zinc ribbon.[12]
The following table[12] shows the different structures and their key features:
Zinc finger, C2H2 type | |||||||||
Identifiers | |||||||||
Symbol | zf-C2H2 | ||||||||
Pfam | PF00096 | ||||||||
Pfam clan | CL0361 | ||||||||
InterPro | IPR007087 | ||||||||
The Cys2His2-like fold group is by far the best-characterized class of zinc fingers and are extremely common in mammalian transcription factors. These domains adopt a simple ββα fold and have the amino acid Sequence motif:[13]
This class of zinc fingers can have a variety of functions such as binding RNA and mediating protein-protein interactions, but is best-known for its role in sequence-specific DNA-binding proteins such as Zif268 (Egr1). In such proteins, individual zinc finger domains typically occur as tandem repeats with two, three, or more fingers comprising the DNA-binding domain of the protein. These tandem arrays can bind in the major groove of DNA and are typically spaced at 3-bp intervals. The α-helix of each domain (often called the "recognition helix") can make sequence-specific contacts to DNA bases; residues from a single recognition helix can contact 4 or more bases to yield an overlapping pattern of contacts with adjacent zinc fingers.
Zinc knuckle | |||||||||
Identifiers | |||||||||
Symbol | zf-CCHC | ||||||||
Pfam | PF00098 | ||||||||
InterPro | IPR001878 | ||||||||
This fold group is defined by two short β-strands connected by a turn (zinc knuckle) followed by a short helix or loop and resembles the classical Cys2His2 motif with a large portion of the helix and β-hairpin truncated.
The retroviral nucleocapsid (NC) protein from HIV and other related retroviruses are examples of proteins possessing these motifs. The gag-knuckle zinc finger in the HIV NC protein is the target of a class of drugs known as zinc finger inhibitors.
The treble-clef motif consists of a β-hairpin at the N-terminus and an α-helix at the C-terminus that each contribute two ligands for zinc binding, although a loop and a second β-hairpin of varying length and conformation can be present between the N-terminal β-hairpin and the C-terminal α-helix. These fingers are present in a diverse group of proteins that frequently do not share sequence or functional similarity with each other. The best-characterized proteins containing treble-clef zinc fingers are the nuclear hormone receptors.
Zinc ribbon
TFIIB zinc-binding | |||||||||
Identifiers | |||||||||
Symbol | TF_Zn_Ribbon | ||||||||
Pfam | PF08271 | ||||||||
Pfam clan | Zn_Beta_Ribbon | ||||||||
InterPro | IPR013137 | ||||||||
The zinc ribbon fold is characterised by two beta-hairpins forming two structurally similar zinc-binding sub-sites.
Fungal Zn(2)-Cys(6) binuclear cluster domain | |||||||||
Identifiers | |||||||||
Symbol | Zn_clus | ||||||||
Pfam | PF00172 | ||||||||
InterPro | IPR001138 | ||||||||
CDD | cd00067 | ||||||||
The canonical members of this class contain a binuclear zinc cluster in which two zinc ions are bound by six cysteine residues. These zinc fingers can be found in several transcription factors including the yeast Gal4 protein.
zf-C2HC | |||||||||
solution structure of a cchhc domain of neural zinc finger factor-1
Identifiers | |||||||||
Symbol | zf-C2HC | ||||||||
Pfam | PF01530 | ||||||||
InterPro | IPR002515 | ||||||||
zf-C2HC5 | |||||||||
Identifiers | |||||||||
Symbol | zf-C2HC5 | ||||||||
Pfam | PF06221 | ||||||||
InterPro | IPR009349 | ||||||||
zf-C2HC | |||||||||
solution structure of a cchhc domain of neural zinc finger factor-1
Identifiers | |||||||||
Symbol | zf-C2HC | ||||||||
Pfam | PF01530 | ||||||||
InterPro | IPR002515 | ||||||||
zf-C2HC5 | |||||||||
Identifiers | |||||||||
Symbol | zf-C2HC5 | ||||||||
Pfam | PF06221 | ||||||||
InterPro | IPR009349 | ||||||||
Various protein engineering techniques can be used to alter the DNA-binding specificity of zinc fingers[13] and tandem repeats of such engineered zinc fingers can be used to target desired genomic DNA sequences.[14] Fusing a second protein domain such as a transcriptional activator or repressor to an array of engineered zinc fingers that bind near the promoter of a given gene can be used to alter the transcription of that gene.[14] Fusions between engineered zinc finger arrays and protein domains that cleave or otherwise modify DNA can also be used to target those activities to desired genomic loci.[14] The most common applications for engineered zinc finger arrays include zinc finger transcription factors and zinc finger nucleases, but other applications have also been described. Typical engineered zinc finger arrays have between 3 and 6 individual zinc finger motifs and bind target sites ranging from 9 basepairs to 18 basepairs in length. Arrays with 6 zinc finger motifs are particularly attractive because they bind a target site that is long enough to have a good chance of being unique in a mammalian genome.[15]
Zinc finger nucleases
Engineered zinc finger arrays are often fused to a DNA cleavage domain (usually the cleavage domain of FokI) to generate zinc finger nucleases. Such zinc finger-FokI fusions have become useful reagents for manipulating genomes of many higher organisms including Drosophila melanogaster, Caenorhabditis elegans, tobacco, corn,[16] zebrafish,[17] various types of mammalian cells,[18] and rats.[19] Targeting a double-strand break to a desired genomic locus can be used to introduce frame-shift mutations into the coding sequence of a gene due to the error-prone nature of the non-homologous DNA repair pathway. If a homologous DNA "donor sequence" is also used then the genomic locus can be converted to a defined sequence via the homology directed repair pathway. An ongoing clinical trial is evaluating Zinc finger nucleases that disrupt the CCR5 gene in CD4+ human T-cells as a potential treatment for HIV/AIDS.[20]
Methods of engineering zinc finger arrays
The majority of engineered zinc finger arrays are based on the zinc finger domain of the murine transcription factor Zif268, although some groups have used zinc finger arrays based on the human transcription factor SP1. Zif268 has three individual zinc finger motifs that collectively bind a 9 bp sequence with high affinity.[21] The structure of this protein bound to DNA was solved in 1991[22] and stimulated a great deal of research into engineered zinc finger arrays. In 1994 and 1995, a number of groups used phage display to alter the specificity of a single zinc finger of Zif268.[23][24][25][26] There are two main methods currently used to generate engineered zinc finger arrays, modular assembly, and a bacterial selection system, and there is some debate about which method is best suited for most applications.[27][28]
The most straightforward method to generate new zinc finger arrays is to combine smaller zinc finger "modules" of known specificity. The structure of the zinc finger protein Zif268 bound to DNA described by Pavletich and Pabo in their 1991 publication has been key to much of this work and describes the concept of obtaining fingers for each of the 64 possible base pair triplets and then mixing and matching these fingers to design proteins with any desired sequence specificity.[22] The most common modular assembly process involves combining separate zinc fingers that can each recognize a 3-basepair DNA sequence to generate 3-finger, 4-, 5-, or 6-finger arrays that recognize target sites ranging from 9 basepairs to 18 basepairs in length. Another method uses 2-finger modules to generate zinc finger arrays with up to six individual zinc fingers.[16] The Barbas Laboratory of The Scripps Research Institute used phage display to develop and characterize zinc finger domains that recognize most DNA triplet sequences[29][30][31] while another group isolated and characterized individual fingers from the human genome.[32] A potential drawback with modular assembly in general is that specificities of individual zinc finger can overlap and can depend on the context of the surrounding zinc fingers and DNA. A recent study demonstrated that a high proportion of 3-finger zinc finger arrays generated by modular assembly fail to bind their intended target with sufficient affinity in a bacterial two-hybrid assay and fail to function as zinc finger nucleases, but the success rate was somewhat higher when sites of the form GNNGNNGNN were targeted.[33]
A subsequent study used modular assembly to generate zinc finger nucleases with both 3-finger arrays and 4-finger arrays and observed a much higher success rate with 4-finger arrays.[34] A variant of modular assembly that takes the context of neighboring fingers into account has also been reported and this method tends to yield proteins with improved performance relative to standard modular assembly.[35]
Numerous selection methods have been used to generate zinc finger arrays capable of targeting desired sequences. Initial selection efforts utilized phage display to select proteins that bound a given DNA target from a large pool of partially randomized zinc finger arrays. This technique is difficult to use on more than a single zinc finger at a time, so a multi-step process that generated a completely optimized 3-finger array by adding and optimizing a single zinc finger at a time was developed.[36] More recent efforts have utilized yeast one-hybrid systems, bacterial one-hybrid and two-hybrid systems, and mammalian cells. A promising new method to select novel 3-finger zinc finger arrays utilizes a bacterial two-hybrid system and has been dubbed "OPEN" by its creators.[37] This system combines pre-selected pools of individual zinc fingers that were each selected to bind a given triplet and then utilizes a second round of selection to obtain 3-finger arrays capable of binding a desired 9-bp sequence. This system was developed by the Zinc Finger Consortium as an alternative to commercial sources of engineered zinc finger arrays. It is somewhat difficult to directly compare the binding properties of proteins generated with this method to proteins generated by modular assembly as the specificity profiles of proteins generated by the OPEN method have never been reported.
This entry represents the CysCysHisCys (C2HC) type zinc finger domain found in eukaryotes. Proteins containing these domains include:
- MYST family histone acetyltransferases[38][39]
- Myelin transcription factor Myt1[40]
- Suppressor of tumourigenicity protein 18 (ST18)[41]
See also
- Zinc finger nuclease
- Zinc finger inhibitor
- Zinc Finger Transcription Factor
- Steroid hormone receptor
- DNA-binding protein
- Sequence motif
- Structural motif
- RING finger domain
- B-box zinc finger
- TAL effector
- Transcription Activator-Like Effector Nuclease
- Krüppel associated box
- ^ a b Klug A, Rhodes D (1987). "Zinc fingers: a novel protein fold for nucleic acid recognition". Cold Spring Harb. Symp. Quant. Biol. 52: 473–82. doi:10.1101/sqb.1987.052.01.054. PMID 3135979.
- ^ Miller J, McLachlan AD, Klug A (June 1985). "Repetitive zinc-binding domains in the protein transcription factor IIIA from Xenopus oocytes". EMBO J. 4 (6): 1609–14. PMC 554390. PMID 4040853.
- ^ Klug A (2010). "The discovery of zinc fingers and their applications in gene regulation and genome manipulation". Annu. Rev. Biochem. 79: 213–31. doi:10.1146/annurev-biochem-010909-095056. PMID 20192761.
- ^ Miller Y, Ma B, Nussinov R (May 2010). "Zinc ions promote Alzheimer Abeta aggregation via population shift of polymorphic states". Proc. Natl. Acad. Sci. U.S.A. 107 (21): 9490–5. Bibcode:2010PNAS..107.9490M. doi:10.1073/pnas.0913114107. PMC 2906839. PMID 20448202.
- ^ Low LY, Hernández H, Robinson CV, O'Brien R, Grossmann JG, Ladbury JE, Luisi B (May 2002). "Metal-dependent folding and stability of nuclear hormone receptor DNA-binding domains". J. Mol. Biol. 319 (1): 87–106. doi:10.1016/S0022-2836(02)00236-X. PMID 12051939.
- ^ Klug A (October 1999). "Zinc finger peptides for the regulation of gene expression". J. Mol. Biol. 293 (2): 215–8. doi:10.1006/jmbi.1999.3007. PMID 10529348.
- ^ Hall TM (June 2005). "Multiple modes of RNA recognition by zinc finger proteins". Curr. Opin. Struct. Biol. 15 (3): 367–73. doi:10.1016/j.sbi.2005.04.004. PMID 15963892.
- ^ Brown RS (February 2005). "Zinc finger proteins: getting a grip on RNA". Curr. Opin. Struct. Biol. 15 (1): 94–8. doi:10.1016/j.sbi.2005.01.006. PMID 15718139.
- ^ Gamsjaeger R, Liew CK, Loughlin FE, Crossley M, Mackay JP (February 2007). "Sticky fingers: zinc-fingers as protein-recognition motifs". Trends Biochem. Sci. 32 (2): 63–70. doi:10.1016/j.tibs.2006.12.007. PMID 17210253.
- ^ Matthews JM, Sunde M (December 2002). "Zinc fingers--folds for many occasions". IUBMB Life 54 (6): 351–5. doi:10.1080/15216540216035. PMID 12665246.
- ^ Laity JH, Lee BM, Wright PE (February 2001). "Zinc finger proteins: new insights into structural and functional diversity". Curr. Opin. Struct. Biol. 11 (1): 39–46. doi:10.1016/S0959-440X(00)00167-6. PMID 11179890.
- ^ a b Krishna SS, Majumdar I, Grishin NV (January 2003). "Structural classification of zinc fingers: survey and summary". Nucleic Acids Res. 31 (2): 532–50. doi:10.1093/nar/gkg161. PMC 140525. PMID 12527760.
- ^ a b Pabo CO, Peisach E, Grant RA (2001). "Design Andselection Ofnovelcys2His2Zincfingerproteins". Annual Review of Biochemistry 70: 313–340. doi:10.1146/annurev.biochem.70.1.313. PMID 11395410.
- ^ a b c Jamieson AC, Miller JC, Pabo CO (2003). "Drug discovery with engineered zinc-finger proteins". Nature Reviews Drug Discovery 2 (5): 361–368. doi:10.1038/nrd1087. PMID 12750739.
- ^ Liu Q, Segal DJ, Ghiara JB, Barbas CF (May 1997). "Design of polydactyl zinc-finger proteins for unique addressing within complex genomes". Proc. Natl. Acad. Sci. U.S.A. 94 (11): 5525–30. Bibcode:1997PNAS...94.5525L. doi:10.1073/pnas.94.11.5525. PMC 20811. PMID 9159105.
- ^ a b Shukla VK, Doyon Y, Miller JC, DeKelver RC, Moehle EA, Worden SE, Mitchell JC, Arnold NL, Gopalan S, Meng X, Choi VM, Rock JM, Wu YY, Katibah GE, Zhifang G, McCaskill D, Simpson MA, Blakeslee B, Greenwalt SA, Butler HJ, Hinkley SJ, Zhang L, Rebar EJ, Gregory PD, Urnov FD (May 2009). "Precise genome modification in the crop species Zea mays using zinc-finger nucleases". Nature 459 (7245): 437–41. Bibcode:2009Natur.459..437S. doi:10.1038/nature07992. PMID 19404259.
- ^ Reynolds IJ, Miller RJ (December 1988). "[3H]MK801 binding to the N-methyl-D-aspartate receptor reveals drug interactions with the zinc and magnesium binding sites". J. Pharmacol. Exp. Ther. 247 (3): 1025–31. PMID 2849655.
- ^ Carroll D (November 2008). "Progress and prospects: zinc-finger nucleases as gene therapy agents". Gene Ther. 15 (22): 1463–8. doi:10.1038/gt.2008.145. PMC 2747807. PMID 18784746.
- ^ Geurts AM, Cost GJ, Freyvert Y, Zeitler B, Miller JC, Choi VM, Jenkins SS, Wood A, Cui X, Meng X, Vincent A, Lam S, Michalkiewicz M, Schilling R, Foeckler J, Kalloway S, Weiler H, Ménoret S, Anegon I, Davis GD, Zhang L, Rebar EJ, Gregory PD, Urnov FD, Jacob HJ, Buelow R (July 2009). "Knockout rats via embryo microinjection of zinc-finger nucleases". Science 325 (5939): 433. Bibcode:2009Sci...325..433G. doi:10.1126/science.1172447. PMC 2831805. PMID 19628861.
- ^ Tebas P, Stein D (2009). "Autologous T-Cells Genetically Modified at the CCR5 Gene by Zinc Finger Nucleases SB-728 for HIV". ClinicalTrials.gov.
- ^ Christy B, Nathans D (November 1989). "DNA binding site of the growth factor-inducible protein Zif268". Proc. Natl. Acad. Sci. U.S.A. 86 (22): 8737–41. Bibcode:1989PNAS...86.8737C. doi:10.1073/pnas.86.22.8737. PMC 298363. PMID 2510170.
- ^ a b Pavletich NP, Pabo CO (May 1991). "Zinc finger-DNA recognition: crystal structure of a Zif268-DNA complex at 2.1 A". Science 252 (5007): 809–17. Bibcode:1991Sci...252..809P. doi:10.1126/science.2028256. PMID 2028256.
- ^ Rebar EJ, Pabo CO (February 1994). "Zinc finger phage: affinity selection of fingers with new DNA-binding specificities". Science 263 (5147): 671–3. Bibcode:1994Sci...263..671R. doi:10.1126/science.8303274. PMID 8303274.
- ^ Jamieson AC, Kim SH, Wells JA (May 1994). "In vitro selection of zinc fingers with altered DNA-binding specificity". Biochemistry 33 (19): 5689–95. doi:10.1021/bi00185a004. PMID 8180194.
- ^ Choo Y, Klug A (November 1994). "Toward a code for the interactions of zinc fingers with DNA: selection of randomized fingers displayed on phage". Proc. Natl. Acad. Sci. U.S.A. 91 (23): 11163–7. Bibcode:1994PNAS...9111163C. doi:10.1073/pnas.91.23.11163. PMC 45187. PMID 7972027.
- ^ Wu H, Yang WP, Barbas CF (January 1995). "Building zinc fingers by selection: toward a therapeutic application". Proc. Natl. Acad. Sci. U.S.A. 92 (2): 344–8. Bibcode:1995PNAS...92..344W. doi:10.1073/pnas.92.2.344. PMC 42736. PMID 7831288.
- ^ Kim JS, Lee HJ, Carroll D (February 2010). "Genome editing with modularly assembled zinc-finger nucleases". Nat. Methods 7 (2): 91; author reply 91–2. doi:10.1038/nmeth0210-91a. PMC 2987589. PMID 20111032.
- ^ Joung JK, Voytas DF, Cathomen T (February 2010). "Reply to "Genome editing with modularly assembled zinc-finger nucleases"". Nat. Methods 7 (2): 91–2. doi:10.1038/nmeth0210-91b. PMID 20111031.
- ^ Segal DJ, Dreier B, Beerli RR, Barbas CF (March 1999). "Toward controlling gene expression at will: selection and design of zinc finger domains recognizing each of the 5'-GNN-3' DNA target sequences". Proc. Natl. Acad. Sci. U.S.A. 96 (6): 2758–63. Bibcode:1999PNAS...96.2758S. doi:10.1073/pnas.96.6.2758. PMC 15842. PMID 10077584.
- ^ Dreier B, Fuller RP, Segal DJ, Lund CV, Blancafort P, Huber A, Koksch B, Barbas CF (October 2005). "Development of zinc finger domains for recognition of the 5'-CNN-3' family DNA sequences and their use in the construction of artificial transcription factors". J. Biol. Chem. 280 (42): 35588–97. doi:10.1074/jbc.M506654200. PMID 16107335.
- ^ Dreier B, Beerli RR, Segal DJ, Flippin JD, Barbas CF (August 2001). "Development of zinc finger domains for recognition of the 5'-ANN-3' family of DNA sequences and their use in the construction of artificial transcription factors". J. Biol. Chem. 276 (31): 29466–78. doi:10.1074/jbc.M102604200. PMID 11340073.
- ^ Bae KH, Kwon YD, Shin HC, Hwang MS, Ryu EH, Park KS, Yang HY, Lee DK, Lee Y, Park J, Kwon HS, Kim HW, Yeh BI, Lee HW, Sohn SH, Yoon J, Seol W, Kim JS (March 2003). "Human zinc fingers as building blocks in the construction of artificial transcription factors". Nat. Biotechnol. 21 (3): 275–80. doi:10.1038/nbt796. PMID 12592413.
- ^ Ramirez CL, Foley JE, Wright DA, Müller-Lerch F, Rahman SH, Cornu TI, Winfrey RJ, Sander JD, Fu F, Townsend JA, Cathomen T, Voytas DF, Joung JK (May 2008). "Unexpected failure rates for modular assembly of engineered zinc fingers". Nat. Methods 5 (5): 374–5. doi:10.1038/nmeth0508-374. PMID 18446154.
- ^ Kim HJ, Lee HJ, Kim H, Cho SW, Kim JS (July 2009). "Targeted genome editing in human cells with zinc finger nucleases constructed via modular assembly". Genome Res. 19 (7): 1279–88. doi:10.1101/gr.089417.108. PMC 2704428. PMID 19470664.
- ^ Sander JD, Dahlborg EJ, Goodwin MJ, Cade L, Zhang F, Cifuentes D, Curtin SJ, Blackburn JS, Thibodeau-Beganny S, et al. (2010). "Selection-free zinc-finger-nuclease engineering by context-dependent assembly (CoDA)". Nature Methods 8 (1): 67–69. doi:10.1038/nmeth.1542. PMC 3018472. PMID 21151135.
- ^ Greisman HA, Pabo CO (January 1997). "A general strategy for selecting high-affinity zinc finger proteins for diverse DNA target sites". Science 275 (5300): 657–61. doi:10.1126/science.275.5300.657. PMID 9005850.
- ^ Maeder ML, Thibodeau-Beganny S, Osiak A, Wright DA, Anthony RM, Eichtinger M, Jiang T, Foley JE, Winfrey RJ, Townsend JA, Unger-Wallace E, Sander JD, Müller-Lerch F, Fu F, Pearlberg J, Göbel C, Dassie JP, Pruett-Miller SM, Porteus MH, Sgroi DC, Iafrate AJ, Dobbs D, McCray PB, Cathomen T, Voytas DF, Joung JK (July 2008). "Rapid "open-source" engineering of customized zinc-finger nucleases for highly efficient gene modification". Mol. Cell 31 (2): 294–301. doi:10.1016/j.molcel.2008.06.016. PMC 2535758. PMID 18657511.
- ^ Smith AT, Tucker-Samaras SD, Fairlamb AH, Sullivan WJ (December 2005). "MYST family histone acetyltransferases in the protozoan parasite Toxoplasma gondii". Eukaryotic Cell 4 (12): 2057–65. doi:10.1128/EC.4.12.2057-2065.2005. PMC 1317489. PMID 16339723.
- ^ Akhtar A, Becker PB (February 2001). "The histone H4 acetyltransferase MOF uses a C2HC zinc finger for substrate recognition". EMBO Rep. 2 (2): 113–8. doi:10.1093/embo-reports/kve022. PMC 1083818. PMID 11258702.
- ^ Kim JG, Armstrong RC, v Agoston D, Robinsky A, Wiese C, Nagle J, Hudson LD (October 1997). "Myelin transcription factor 1 (Myt1) of the oligodendrocyte lineage, along with a closely related CCHC zinc finger, is expressed in developing neurons in the mammalian central nervous system". J. Neurosci. Res. 50 (2): 272–90. doi:10.1002/(SICI)1097-4547(19971015)50:2<272::AID-JNR16>3.0.CO;2-A. PMID 9373037.
- ^ Jandrig B, Seitz S, Hinzmann B, Arnold W, Micheel B, Koelble K, Siebert R, Schwartz A, Ruecker K, Schlag PM, Scherneck S, Rosenthal A (December 2004). "ST18 is a breast cancer tumor suppressor gene at human chromosome 8q11.2". Oncogene 23 (57): 9295–302. doi:10.1038/sj.onc.1208131. PMID 15489893.
External links
- C2H2 family at PlantTFDB: Plant Transcription Factor Database
- McDowall J. "Protein of the Month: Zinc Fingers". European Molecular Biology Laboratory - European Bioinformatics Institute (EMBL-EBI). Retrieved 2008-01-13.
- Goodsell DS. "Molecule of the Month: Zinc Fingers". Research Collaboratory for Structural Bioinformatics (RCSB) Protein Data Bank (PDB). Retrieved 2008-01-13.
- The double helix between the zinc finger
- Zinc Finger Tools design and information site
- Human KZNF Gene Catalog
- Zinc finger C2H2-type domain in PROSITE
- Entry for zinc finger class C2H2 in the SMART database
- The Zinc Finger Consortium
- ZiFiT- Zinc Finger Design Tool
- Zinc Finger Consortium Materials from Addgene
- Predicting DNA-binding Specificities for C2H2 Zinc Finger Proteins
This page is based on a Wikipedia article. The text is available under the Creative Commons Attribution/Share-Alike License.
This tab holds the annotation information that is stored in the Pfam database. As we move to using Wikipedia as our main source of annotation, the contents of this tab will be gradually replaced by the Wikipedia tab.
Zinc-binding RING-finger Provide feedback
This RING/U-box type zinc-binding domain is frequently found in the catalytic subunit (irx3) of cellulose synthase. The enzymic class is EC:220.127.116.11, whereby the synthase removes the glucose from UDP-glucose and adds it to the growing cellulose, thereby releasing UDP. The domain-structure is treble-clef like ( PDB:1weo).
Internal database links
SCOOP: | zf-RING_4 |
Similarity to PfamA using HHSearch: | zf-RING_4 |
External database links
Pseudofam: | PF14569 |
SYSTERS: | zf-UDP |
This tab holds annotation information from the InterPro database.
No InterPro data for this Pfam family.
Domain organisation
Below is a listing of the unique domain organisations or architectures in which this domain is found. More...
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Pfam Clan
This family is a member of clan RING (CL0229), which has the following description:
This clan includes the Ring zinc finger domains as well as the U-box domain that appears to have lost the zinc coordinating cysteine residues [1].
The clan contains the following 24 members:
Baculo_RING FANCL_C Prok-RING_1 Prok-RING_2 Prok-RING_4 RINGv Rtf2 U-box zf-Apc11 zf-C3HC4 zf-C3HC4_2 zf-C3HC4_3 zf-C3HC4_4 zf-MIZ zf-Nse zf-rbx1 zf-RING-like zf-RING_2 zf-RING_4 zf-RING_5 zf-RING_6 zf-RING_UBOX zf-UBP zf-UDPAlignments
We store a range of different sequence alignments for families. As well as the seed alignment from which the family is built, we provide the full alignment, generated by searching the sequence database using the family HMM. We also generate alignments using four representative proteomes (RP) sets, the NCBI sequence database, and our metagenomics sequence database. More...
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We make a range of alignments for each Pfam-A family. You can see a description of each above. You can view these alignments in various ways but please note that some types of alignment are never generated while others may not be available for all families, most commonly because the alignments are too large to handle.
Seed (6) |
Full (380) |
Representative proteomes | NCBI (405) |
Meta (0) |
Jalview | ||||||||
PP/heatmap | 1 | |||||||
Pfam viewer |
1Cannot generate PP/Heatmap alignments for seeds; no PP data available
Key: available, not generated, — not available.
Format an alignment
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We make all of our alignments available in Stockholm format. You can download them here as raw, plain text files or as gzip-compressed files.
Seed (6) |
Full (380) |
Representative proteomes | NCBI (405) |
Meta (0) |
Raw Stockholm | ||||||||
Gzipped |
You can also download a FASTA format file containing the full-length sequences for all sequences in the full alignment.
External links
MyHits provides a collection of tools to handle multiple sequence alignments. For example, one can refine a seed alignment (sequence addition or removal, re-alignment or manual edition) and then search databases for remote homologs using HMMER3.
HMM logo
HMM logos is one way of visualising profile HMMs. Logos provide a quick overview of the properties of an HMM in a graphical form. You can see a more detailed description of HMM logos and find out how you can interpret them here. More...
This page displays the phylogenetic tree for this family's seed alignment. We use FastTree to calculate neighbour join trees with a local bootstrap based on 100 resamples (shown next to the tree nodes). FastTree calculates approximately-maximum-likelihood phylogenetic trees from our seed alignment.
Note: You can also download the data file for the tree.
Curation and family details
This section shows the detailed information about the Pfam family. You can see the definitions of many of the terms in this section in the glossary and a fuller explanation of the scoring system that we use in the scores section of the help pages.
This family is new in this Pfam release.
Seed source: | Jackhmmer:A9T9M4 |
Previous IDs: | none |
Type: | Domain |
Author: | Coggill P |
Number in seed: | 6 |
Number in full: | 380 |
Average length of the domain: | 75.90 aa |
Average identity of full alignment: | 53 % |
Average coverage of the sequence by the domain: | 8.56 % |
HMM information
HMM build commands: |
build method: hmmbuild -o /dev/null HMM SEED
search method: hmmsearch -Z 23193494 -E 1000 --cpu 4 HMM pfamseq
Model details: |
Model length: | 80 | ||||||||||||
Family (HMM) version: | 1 | ||||||||||||
Download: | download the raw HMM for this family |
Species distribution
Sunburst controls
ShowThis visualisation provides a simple graphical representation of the distribution of this family across species. You can find the original interactive tree in the adjacent tab. More...
Tree controls
HideThe tree shows the occurrence of this domain across different species. More...
Please note: for large trees this can take some time. While the tree is loading, you can safely switch away from this tab but if you browse away from the family page entirely, the tree will not be loaded.
For those sequences which have a structure in the Protein DataBank, we use the mapping between UniProt, PDB and Pfam coordinate systems from the PDBe group, to allow us to map Pfam domains onto UniProt sequences and three-dimensional protein structures. The table below shows the structures on which the zf-UDP domain has been found. There are 1 instances of this domain found in the PDB. Note that there may be multiple copies of the domain in a single PDB structure, since many structures contain multiple copies of the same protein seqence.
Loading structure mapping...


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Joseph Bentley Digging Fork
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- Polished Stainless Steel Head
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Please note that this item comes with possible health and safety hazards. Refer to the container for full information and always follow the instructions.
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text:
Dark Cherry Back Binding post connectors Dark Cherry (Grille On) Grille off Midrange/tweeter array View larger THIEL CS3.7 Floor-standing speaker (Dark cherry) Item #: 684CS37DC 4 Reviews This item is no longer available. Original Price: $6,999.99 each Shop all Floor-standing Speakers Need help? 1.888.955.6000 Email Chat Ask an expert advisor No pressure, no commission — just lots of good advice from our highly trained staff.
by Chris Egner THIEL's best tower speakers THIEL designed the CS3.7 with one idea in mind — to provide the listener with the most realistic and musical sound reproduction possible. To achieve this goal, THIEL loaded the CS3.7 with cutting-edge technologies while paying careful attention to each and every detail of design and construction. These speakers won the Consumer Electronics Show's 2007 Innovations Design and Engineering Award — THIEL's 22nd such award in their 31-year history. With the CS3.7, THIEL took everything they've learned over the past 31 years to create one of the finest speakers available today. Unsurpassed sound with impressive midrange clarity An aluminum woofer delivers deep, punchy bass. The secret to the CS3.7's incredibly lifelike sound starts with a newly designed midrange driver. The aluminum diaphragm features ridges extending from the center to the outer edge. These ridges add strength to the driver, giving it far greater stiffness than typical diaphragms, or even those made from exotic materials. This design achieves such great stiffness that the midrange continues to operate perfectly all the way up to 20 kHz. The result? An open, transparent sound that includes every nuance of the original source material. Remarkably clear highs A high-output aluminum tweeter produces crisp highs, thanks in part to a unique motor structure that includes a total of five neodymium magnets. The CS3.7 houses the tweeter inside the midrange to maintain the proper time alignment, for unparalleled accuracy and realism. You'll enjoy crystal-clear sound with pinpoint imaging and an exceptionally wide, deep soundstage. Richly detailed, musical bass Bass comes courtesy of an aluminum woofer and passive radiator, each with a die-cast chassis. A motor structure consisting of a short, 3" diameter voice coil sitting inside a large magnet effortlessly drives the woofer. Unlike the voice coils found in most speakers that extend outside the magnet, the deliberately short voice coil always "sees" the optimum magnetic field as it travels back and forth. This eliminates distortion caused by variances in magnetic strength. The passive radiator increases the speaker's output. It all adds up to accurate, musical bass that perfectly complements the treble without being "boomy" or overbearing. The advanced crossover ensures that your music is faithful to the original signal. State-of-the-art crossover The "unsung hero" of any speaker is the crossover — it directs the right frequencies to the right drivers so you don't have a tweeter trying to reproduce bass and a woofer trying to reproduce highs. THIEL meticulously engineered their first-order crossover to achieve tonal neutrality. The advanced crossover ensures that musical information such as amplitude, phase, and time remain faithful to the original signal. An exquisitely crafted cabinet The super-strong cabinet features 15 layers of hardwood, laminated together under high pressure. Curved edges reduce unwanted diffraction while creating non-parallel surfaces to minimize sound-muddying internal resonance. The formed aluminum cabinet top provides enormous strength, virtually eliminating unwanted vibration. Its rounded shape around the midrange eliminates diffraction of energy from the mid and tweeter, and helps minimize resonances within the cabinet. The machined aluminum front baffle is more than 30 times as strong as the usual MDF to provide an incredibly rigid mounting surface for the drivers. The sloping angle of the baffle also positions the voice coils of all three drivers on the same plane. This allows all the frequencies to reach your ears at the same time, giving you the most complete and accurate sound reproduction possible. Got wire? Most speakers don't include any. Learn about choosing and installing speaker wire at the Crutchfield Learning Center. Shop our selection of speaker wire. Product Highlights:recommended power 100-600 wattsfrequency response 33-26,000 Hz (±2dB)4-ohm impedance suited for high-current amplifierssensitivity 90 dB1" aluminum dome tweeter with neodymium magnet structure4-1/2" wave-shaped, aluminum midrange driver10" wave-shaped, aluminum woofer10" passive radiatormachined aluminum front bafflesloped cabinet provides time coherence between woofer and midrange/tweeter arraybinding post connectors12-1/2"W x 45"H x 21"Dwarranty: 10 yearsOur 60-day money-back guaranteesold individually Floor-standing speakers still standing tall Sure, floor-standing tower speakers buck the industry trend toward smaller, visually inconspicuous speakers, but they're hard to beat if great sound is your primary goal. Tower speakers produce powerful sound with excellent bass, making them a great choice for serious music lovers and for folks who want high-impact home theater.
CS3.7 issues Written By David S., Silver Lake Ohio on Monday, July 23, 2012 I helped set up my brothers new CS3.7 speakers. He has a NAD C375BEE amp with the NAD preamp. Initially I was surprised how tinny the sound was and the depth of the sound seemed flat. I understood there was a break-in period, but I had doubts that there would be enough improvement to compensate from what I heard initially. My brother called me after he had about 300 hours on them and I was surprised at the improvement, but it still wasn't up to snuff. We replaced the Monster cables with upgraded cables and then added a Klipsch S-112 subwoofer. The difference from what we heard initially was amazing. We can't push the amp too hard, but at moderate sound levels I don't know when I have heard speakers this full and rich. They are very responsive with no noticeable lag. Orchestral horns are so impressive that hearing them live would be a disappointment. I have a fair amount of studio experience over my 60+ years and these speakers rival any reference speakers I remember.. They are simply magnificent. Just one word of caution, the speakers will will expose weaknesses in your system you never thought you had and they need a few hundred hours to season. Pros: Simply Glorious sounds, accurate and rich. This is a benchmark speaker that will thrill any audiophile. Cons: Requires 300+ hours of break-in to season the sound. They will reveal flaws in your system and you might need an amp and cable upgrade to discover what these speakers are capable of.
Allow for break-in!! Written By Aaron, Charlotte, NC on Wednesday, April 21, 2010 Fresh out of the box, these speakers are almost unbearable. I've read all over about the break-in and how irritating they can be at first, but I had no idea that they were this bad. Definitely bordering on shrill. But alas, FINALLY (I'm guessing 100-150 hrs.) they are really coming into their own and settling down. (I put a CD on loop when I left for work.) That being said, they are now GLORIOUS. And what gives me goosebumps is that I know I have another 50-100 hours to go before they really open up. Coming from a high-end set-up, I forgot how easy and satisfying a simple solid state set-up can be. To echo others, make sure you have the juice to run these. I currently run a 250 watt amp with T-14's cables and even though it sounds fine so far, I can tell the Thiel's can take so much more. (Plans to upgrade both amp and cables in the future.) The only thing I would probably improve on is the bass. While fine for most, bass lovers will be disappointed. I always play Sigur Ros's "Saeglopur" off of the album "Takk" and when the drums kick in is how I judge the bass. Definitely lacking some there. (Plans to add two JL Audio Fathom F112's in the future. I borrowed my brother's Velodyne's and WOW.) While no speaker will ever be perfect, throw in two subs, and these IMO are about as close as you'll ever get.
Initial Review Written By EoFeb2008, San Jose, CA on Sunday, February 10, 2008 Excellent value for music and home theater application. Furniture grade construction, beautifully done. Excellent sensitivity and able to fill a large 25x30 room with ease. For best performance, requires a top notch audiophile amplifier that has at least 150W or more. This review is based on such amplifiers. Disappears and leaves a sound stage that is deeper and wider beyond the speakers. Has excellent imaging when set up properly. Beautiful highs, no harshness. Sweet mid-range and excellent bass articulation. Excellent uniformity from the highs to the low. Very neutral but not cold analytical and has no overt character of its own. If you use mediocre source components or poor cables, these speakers will expose the weaknesses of these components without mercy. Works wonderfully with higher power ARC tube amplifiers. Our speakers have approximately 48+ hours of break-in time as of this writing so their voices are not yet fully open. Will report back after 200+ hours when there is more information.
AWESOME!!!!!!!!!!!! Written By jmccoy, Los Vegas, NV on Tuesday, September 04, 2007 THIS IS THE SPEAKER SET TO BUY. AMAZING AUDIO QUALITY. TOTALLY WORTH THE 6 PAYCHECKS FOR THEM. VERY LOUD CLEAN BASS AND SOUND STAGE. I WISH I COULD FIND A BIGGER AMP TO PUSH THESE BABIES. GET THEM NOW, THESE SPEAKERS YOU WILL FEEL IN YOUR CHEST!!! IN A FEW MONTHS I CAN AFFORD ANOTHER PAIR. YESSSSSSSSSSS.


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I'm sure there was some positive feed back in there
Probably on the lines of more collaboration like at the Desktop Summit (that's what I put so I know it's in there)
PS I'm a KDE User but still filled it in
Phoronix: It's Easy To Guess What Angers GNOME Users
The 2011 GNOME User Survey, an end-user survey that was assembled by independent GNOME users and hosted on Phoronix, began less than 24 hours ago and we're already approaching 2,000 submissions. There's still one month to go, and from these submission so far when simply dumping the comments it amounts to about 148 pages. However, it's not hard to guess what most of these comments are about when it comes to the GNOME desktop...
I'm sure there was some positive feed back in there
Probably on the lines of more collaboration like at the Desktop Summit (that's what I put so I know it's in there)
PS I'm a KDE User but still filled it in
+1 Gnome developers are assholes.
(Sorry for trolling. I am not the troll type. I really not used to call anybody ***hole. I know its rude, very rude. But they deserve it).
And really no explanation can hold up when they remove ability to choose between hibernate and suspend option. Its 0 maintenance. Only being an ***hole can explain that. G-shell plugin that add it back doesn't count.
If they make a dumb-down g-shell plugin that would remove even all options I am fine with that but not the other way around.
And even if they finally accept revival of some of removed options that won't make them any better.
Really switch to KDE. No matter how buggy/slow it is. It is where freedom lies.
Last edited by Maxim Levitsky; 10-18-2011 at 08:00 AM.
Yikes! As someone who really likes gnome shell I hope gnome dev don't listen to the masses. :P
Disclaimer: Happy gnome user here, was a happy KDE user until 1 month ago, then wanted a change
What's to bet that the same people moaning about Gnome 3 and pointing out how great KDE is are the same people who hated on KDE4 when it game out and pointed out how great gnome was.
Haters gotta hate.
If you want to improve anything you might consider providing constructive feedback that provides details and suggestions instead of bitching about everything.
This article and its forum discussion is another excellent example of the degeneration of Phoronix
whats the problem? I mean what really sucks, is that ubuntu does not support gnome-shell properly, they dont include some packages that are in the gnome 3.2 release, and they dont make a kind of good theme for it etc, because they wanna force the users to use the much more hated unity desktop.
Because of that, the user Experience for gnome-shell is worse than it could be. Gnome2.x did also only shine on distries that did support it well and did preinstall a good default theme, therefor ubuntu was famous.
Besides that, gnome-shell needs some love but the ideas and concepts of it are really good and neccessary. gnome 2.x is done if you like it use it there is no need to make it better, it does most stuff like it should.
So users that are crying now should just use older linuxes or install somehow the gnome2.x packages in their distries. Why do all people need to update their linux all 6 months you need that if you wanna see such new ui or if you did buy a new hardware and the drivers for the old linux are bad, but even than there are backports of the new kernels for the older distries. So just use a old distri and wait a few minor-releases before you switch to gnome-shell.


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Dells native to lead state medical association
Dell Rapids native Dr. Daniel J. Heinemann recently became the 132nd president of the South Dakota Medical Association.Now living in Canton, Heinemann was elected to serve as SDMA president during the organization's annual meeting May 31 in Sioux Falls. Outgoing president Robert L. Allison, MD, of Pierre presented the presidential medallion to Heinemann at the SDSMA's Presidential Inauguration Banquet at the Ramkota Hotel."I look forward to the coming year at SDSMA as we face the many challenges to the practice of medicine," Heinemann said.A Dell Rapids St. Mary High School graduate, Heinemann received his medical degree from the University of South Dakota School of Medicine and completed his residency at Sioux Falls Family Medicine. Upon completion of his training, he established a new medical practice in Canton called Canton Family Physicians, where he practiced for 20 years. He currently is a chief medical officer for Sanford Health Network and Sanford Clinic. During his first years at Sanford Health, Heinemann worked in the Office of Health Policy.Heinemann has been a member of the SDSMA since 1983. He has a great deal of experience in the areas of legislative affairs, quality and patient safety. He has been involved in the leadership of organized medicine in South Dakota for several years and is a member of the American Medical Association, the South Dakota Medical Directors Association, the American Academy of Family Physicians and the South Dakota Academy of Family Physicians.Prior to becoming SDSMA president, Heinemann has held offices within the SDSMA's Executive Committee, as well as serving as a Councilor for the Seventh District Medical Society. Heinemann has also volunteered for the SDSMA's advocacy efforts during the legislative sessions by participating in the SDSMA Doctor of the Day program.
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It is important to make sure the home of someone who has dementia is safe for them.
See also:
Wandering can be a serious problem in people who have more advanced dementia. These tips may help prevent wandering:
To prevent harm when someone with dementia does wander:
Inspect the person's house, and remove or reduce hazards for tripping and falling. See also: Preventing falls
Do not leave a person who has advanced dementia alone in the home.
Lower the temperature of the hot water tank. Remove or lock up cleaning products and other items that may be poisonous.
Make sure the kitchen is safe.
Remove, or store in locked areas:
Knopman DS. Alzheimer's disease and other dementias.In: Goldman L, Schafer AI, eds. Cecil Medicine. 24th ed. Philadelphia, Pa: Saunders Elsevier; 2011:chap 409.
Smith DA, Brechtelsbauer DA. Delirium and dementia. In: Rakel RE, ed. Textbook of Family Medicine. 8th ed. Philadelphia, Pa: Saunders Elsevier; 2011:chap 48.
Moore, HD, Algase DL, Powell-Cope G, Appelgarth S, Beattie ER. A framework for managing wandering and preventing elopement. Am J Alzheimers Dis Other Demen. 2009 Jun-Jul;24(3):208-19.
Dave J, Hecht M. Dementia. In: Frontera, WR, Silver JK, Rizzo TD Jr, eds. Essentials of Physical Medicine and Rehabilitation. 2nd ed. Philadelphia, Pa: Saunders Elsevier; 2008:chap 119.
Petersen RC. Mild cognitive impairment. N Engl J Med. 2011;364:2227-2234.
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If you are a commercial property owner, investor, or manager, you tend to have a lot on your plate when it comes to maintenance. Certainly, you want your building or complex to look its best, but at the same time, it is essential to keep in mind the bottom line. See full article.
By Melissa CrawleyMore Content Now
I first fell in love with felt Advent calendars about six years ago when I saw a Christmas tree with 25 superbly decorated pockets at Pottery Barn.
WEEK IN RELIGIONAn evangelical Muslim preacher is being investigated by Pakistani police for allegedly making blasphemous comments about Islam in a viral video that began circulating earlier this month.
It's hard to believe that we are gearing up for the end of 2014. I know Christmas isn't here yet, but the planning for the New Year's Eve Anchor Drop in Festival Square is all I'm thinking about these days. As I...
A Cheboygan man who has spent countless hours volunteering his time to help young hunters learn to participate in the sport safely, has been named the 2014 Hunter Education Volunteer Instructor of the Year by the Michigan Department of...
The Cheboygan County Road Commission has not received good news from the company that is outfitting its new plow trucks for use this winter. Outgoing road commission manager Mike Roper said he had received correspondence from Truck and...
Tuscarora Township's Marina Park Committee recently held a special meeting to open bids for its trailhead project. The committee received two bids for their project — one from Reith Riley Construction, with corporate...
Recommendations recently were made to the Cheboygan County Board of Commissioners by the Board of Appointments and Procedures to fill vacant positions on several county committees and boards. The commissioners chose to approve all of the...
Ruth J. Turi, age 94, of Black Lake (Cheboygan), died on Monday, Dec. 15, 2014 at Hospice House in Cheboygan.
A visual step-by-step that will teach you how to glaze a holiday ham from start to finish.
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Finding A School Has Never Been Easier.
If you're looking for a degree or certificate program, you've come to the right place. Yahoo Education gives you access to an education database that can help you find a school and program that is right for you.
Whether you want to study at a campus near you or online, we've got hundreds of programs to choose from.
Get started now by choosing your area of interest... and good luck with your education!


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wake up wake up sleepy head your already late...................:)
Photo caption 003
Maybe a lady became a dog..............?
I won't let you sleep in my bed!
Oh my god!........I transfered into a DOG from a HUMAN BEING!!!!!!!!!!!!!!!!
this dog is lucky.
Please don't call me dog!!!....
Sleepyhead. :(
We think this dog is cute but i think its body very painful.
your breakfast is ready.
she's a super star of the dog
she's going to meet her boy friend 6 o'clock
I love my bed 2 sleep but the alarm clock always disturb me...damnn...
who put that alarm clock beside me??
so funny
Now it's time to get up from bed.
I love this alarm clock except it's voice(noise actually)... breaks my concentration while I'm investigating on sweet dreams...
Can i sleep a little longer..............................
Curly haired lady with beard !!!!!!!!!!!!!!!!!!
It is a lady or a dog?
doggywood star
i love it so cute
It's time for my beauty sleep.
miss fashioniesta


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Hello, all!
Looking to significantly thin the fleet of subs that I've collected over the years. I need the cash and the space for other projects, unfortunately. The following is a list of all of the boats that I have available for sale along with their prices. I also have quite a few Engel piston tanks as well as a selection of blueprints.
Additional information and photos can be seen on my site at: http://rc-sub.com/projects/viewproject.php5?id=45
Please EMAIL ME. Do not use the board PM system. Email address is "bob(at)rc-sub.com"
Here is the list:
-OTW Type VII: $700
-EFX Disney Nautilus rework: $1,950
-Total Immersions Nautilus $380
-Total Immersions Nautilus: $380
-DeBeor Seaview 58": $500
-DeBoer Seaview Concept 2: $300
-Russian Typhoon 1/150 scale: $300
-Revell Type VII with conversion kit: $100 (2 available)
-Brodeen 48" Nautilus model: $650
-Engel Gato hull kit only: $550
-Stan Sanders 7" Nautilus hull kit: $750
Blueprints: ($40 each including shipping)
-1/96th scale Russian Typhoon (detailed)
-1/96th scale Russian Typhoon (basic)
-1/72nd scale Disney Nautilus prints (4 set) by Lee Seiler
-Engel Nautilus blueprint set
-1/96th scale Akula II
-1/72 scale Los Angeles class
-47.5" Nautilus rivet pattern guide
-1/72 scale Skipjack (Deep Sea Designs)


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Rockies, Padres seek to regroup after series losses
The Colorado Rockies were rolling until they met with last year's World Series champions, the San Francisco Giants. Then they ran into the wall with a thud.
After compiling a five-game winning streak, the Rockies were swept in San Francisco and were outscored 23-8.
Their latest loss was a 10-0 blowout on Wednesday.
"It wasn't a good day for us as a team, but we've got fight in us," starter Jeff Francis said. "We'll shake it off and we'll go try to take it to the Padres on the weekend."
If the Rockies have fight in them, so do the Padres.
San Diego got into a benches-clearing fracas against the Dodgers in the sixth inning on Thursday night. The incident broke out when Padres outfielder Carlos Quentin charged the mound after he was hit by a pitch from Dodgers starter Zack Greinke.
Quentin was ejected from the game, along with Dodgers outfielders Matt Kemp and Jerry Hairston Jr. Quentin's actions did little to rally the Padres, who lost 3-2, but Greinke suffered a fractured left collarbone.
San Diego is last in the National League West at 2-7. The Padres have been hurt by poor starting pitching, with their starters averaging fewer than five innings per start.
"It's still early, but you don't like to see this," manager Bud Black said. "We're going to keep an eye on this. We need to get some length out of our starters."
Tyson Ross, who will start on Friday in the opener of a three-game series at Petco Park, did not make it out of the fifth inning in his season debut against the Rockies on April 6. The right-hander needed 96 pitches to get 12 outs. Ross allowed three runs on only three hits, but was hurt by four walks.
The Rockies swept the Padres during a three-game series in Colorado last weekend. Colorado outscored San Diego 20-6.
Rockies: Rotation rocked after great start
• After starting the season with a 3.02 ERA and a 4-1 record in the team's first six games, the Colorado starters struggled in San Francisco. As a whole, they allowed 11 earned runs in 12 2/3 innings for a 7.82 ERA.
• Jon Garland is scheduled to start for the Rockies on Friday, which will be only his second appearance since June 1, 2011. He threw just 75 pitches in six innings against the Padres on April 6, allowing two runs on five hits.
• It should surprise no one that the Rockies are tied for the NL lead in home runs, especially considering their batter-friendly park in Colorado. It is surprising, however, that only five of those 15 homers have come at home.
Padres: Hundley sees results from swing adjustment
Padres catcher Nick Hundley worked hard during the offseason with hitting coach Phil Plantier to change his swing, and he's gotten positive results early in 2013.
Hundley shortened his swing, and has six hits in his past two games. The catcher tied his career high with four hits on Wednesday. He went 2-for-4 with a double on Thursday.
"Being short [to the ball] has been huge for me," Hundley said of having a more compact swing. "I think that I was getting myself into trouble when my hands get too far away from my body."
While the sample size is still small, Hundley has seven hits over his last 11 at-bats, compared to two hits in his first 15 at-bats. He's hitting .346 with one home run and two RBIs.
Worth noting
• The Padres are 1-5 at home this season, while the Rockies are 2-4 on the road.
• San Diego has allowed 50 runs, the second most in the NL behind Philadelphia (54).
Evan Peaslee is an associate reporter for MLB.com. This story was not subject to the approval of Major League Baseball or its clubs.


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Your Apple Store search request had no matches. Please double check that all words are spelled correctly or try different (more general) keywords. For your assistance, we've provided links to commonly requested information below.


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Diana C.Centennial, COOutstanding QualityLooks like all of the reviews for this Mehaz cuticle pusher are old, so I thought it was time to add a new one. I just received mine yesterday and tried it for the first time. I admit that I hesitated to buy it because of the price, but now I wish I hadn't waited so long. I have never seen a higher-quality implement. The surgical-quality stainless steel is shiny and substantial (one reviewer said it should have been shinier, which I can't understand), and the rounded pusher ends are perfectly polished and completely smooth. It has already made a difference in the look of my cuticles. Don't settle for lower-quality pushers that could cause damage to your nails--spring for the best!posted Jun 20, 2013Link to this post
Mariet Vandenmunckhof-vedderGeorgia's Emerald CityMehaz Cuticle Pusher Is The Most ErgonomicAfter I lost finally the one that I used for decades, aside from some manicure sets over the years; this is IT. It is easy to handle, perfect grip and it does the job perfectly! Nothing more to add. Besides the super service you get from this company; very courteous!!!posted Jun 9, 2008Link to this post
MemuTexasWish This Would Go On SaleI really didnt want to spend this much money on a cuticle pusher but I didnt like getting my nails scratched up like it does when I used my old nail file so I figured why the heck not. It takes a bit more effort but I am happy with this cuticle pusher. Truly does not scratch my nail. I would recommend getting a cuticle remover though to soften up the nail before hand, but other than that I would recommend this product. I just wish it would go on sale so I could get another one as a spare without having to dish out $20.posted Mar 24, 2008Link to this post
KatielynneAstoria, OregonQuality, Quality, QualityIf youre reading this, you probably truly need a tool to efficiently rid your nails of ever-growing cuticles. This tool does what it sets out to do and more easily than youd expect. I was pleasantly surprised with its performance; I grew up using a pusher that wasnt good quality, but used it until it was lost because I didnt know the difference. Dont hesitate to give this tool a chance...youll notice immediately how well it works and its high quality. It makes caring for your nails a pleasure instead of a job!posted Sep 30, 2007Link to this post
JoanBoston, MA.Mehaz Cuticle PusherI just love this Mehaz cuticle pusher. Its stainless steel and feel well balanced in the hand. The pushers are wide enough to make it easy, and they really grab hold and push the cuticle down. Ive had other metal pushers but they were narrow, so would slip and bruse. Ive always had problems with my cuticles and this, combined with the Qticle cream, solved a life-long aggravation!posted Aug 1, 2007Link to this post
SallyCarmel, INExcellent Product!I just received my Mehaz cuticle pusher in the mail and couldnt wait to use it! It works soooo much better than orange sticks or other pushers. Im impressed with the quality of the product and the lifetime guarantee. Even though this is for home use (as opposed to a nail salon) Im glad that it can be sanitized with hot water and soap without rusting. My cuticles look much much better now that Ive used the Mehaz!posted Jan 12, 2007Link to this post
PatriciaIllinoisCuticle ImplementIve always wanted this professional implement and now I have it! I use it daily when I am not wearing nail polish. This, along with a "buffing" treatment has made a noticable difference with my nails.posted Sep 7, 2006Link to this post
CintiaArlington, VABest Cuticle Pusher In The MarketThis is certainly a professional tool. It can be used in nails or toenails easily, great material, perfect shape and the best is that it does not peel off your nail, as most metal cuticle pushers do.posted Dec 9, 2005Link to this post
Kathryn AndersenMehaz Cuticle PusherI was disappointed in the metal used. I had hoped it would be lighter and shinier.posted Jun 21, 2005Link to this post
JoanneMichiganMehaz Cuticle PusherCant say enough about this manufacturer and the high quality. Its the best.posted Feb 18, 2005Link to this post
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Screwdriver supplied. Very nice gesture; it is yours, we have faith in our customers and you have the freedom to do with it what you want.
Cardboard box. Minimalistic. No sea of plastic; we care about the environment.
I will remember this company. However; nVidia... *ouch*. It would be better to ship it with a much more lower power consuming ATi card. Not to mention FLOSS. But given that they invested real world money I do not blame them for supplying a system that realy works *now*. For a lot of people it is all out proprietary Windows with a little Firefox versus almost all out FLOSS with a little bit of proprietary nVidia.


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These are the three important messages that more than a quarter-million students have heard from Joe Cunningham, Al Hrabosky, Andy Benes, Mike Claiborne and Fredbird as part of the Cardinals' School Program. This traveling, cost-free program, brought to you by Fox Sports Midwest, entertains students and reminds them of life's important goals.
The 50-minute program, which includes a video presentation and recorded Cardinals player messages, is designed for grades K-6. The interactive assembly encourages students to participate and gives all kids the chance to win prizes.
The 2014-2015 school year is full. We will accept applications for the 2015-2016 school year.
Once your application has been received, a Cardinals' Community Relations representative will call the principal to see if a visit can be arranged. Thanks again for your interest in the Cardinals!
Note: The program is available to schools within a 75-mile radius of St. Louis, MO.


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Council could scale back $8.25 million budget
A proposed $8.25-million City budget has local officials calling for major changes to the 2014 agenda.The first draft of the 2014 budget calls for a 27 percent increase from the $6.46-million budget the City is operating with this year. The budget includes projects like the $1.9 million utility extension, the $615,000 Garfield Bridge replacement and interest payments for a $1-million loan for Dell Rapids Economic Development Corporation.Although the plan calls for some cuts to some civic groups and contracted county services, Councilor Mark Crisp says there's too much in it, and encouraged his fellow councilors to consider cutting deeper.Crisp said projects like the utility extension and DREDC's development park plans are investments and should be pursued. But the current budget calls for finding increased revenue – whether it's raising utility rates or taxes – and that's unacceptable, he said."There's a lot of things that I look at in here and I think, 'you've got to be kidding me,'" a visibly agitated Crisp said at the council's Sept. 2 meeting. "We're supposed to pass this through, but in the mean time we're having tax increases and we're having three years worth of water rate increases."The people don't appreciate it and the people aren't going to tolerate it," he added. "Every item in this budget needs to be looked at. This budget is a train wreck waiting to happen."The 2014 plan calls for cuts to law enforcement and the Dell Rapids Chamber of Commerce.After the Minnehaha County Sheriff's Department announced it was raising its rates by 3.8 percent, the City Council expressed a desire to reduce services in order to keep costs the same. With Councilor Chad Andrews the lone nay vote, the City Council approved a plan last week to chop six hours per week from the time the sheriff's department must have officers in Dell Rapids. The budget calls for a $5,500 cut to the Dell Rapids Chamber of Commerce, which has been in rebuilding mode since its board dissolved in 2012. The economic group got $13,500 in taxpayer dollars that year and this year, but Crisp and other councilors say Dell Rapids hasn't seen enough results to justify maintaining that level of funding."I personally would like to see the chamber get rebuilt and I would like to see some results from what the chamber is doing," Crisp said. "But we have some tough decisions. Most people don't want their taxes to go up."Councilor Lee Burggraff agreed."I was the president of the chamber for 10 years. At that time, we didn't have any trouble," he said. "We had strong leadership and we had a $50,000 budget and we raised all of it."The little money received from the City, he said, was donated to DREDC.The Council, required to adopt next year's budget no later than Oct. 1, was set to meet Monday for a special meeting to revisit the proposed budget.
Argus Leader
Dell Rapids Public School
Dell Rapids St. Mary's Catholic School
Dell Rapids Chamber of Commerce
City of Dell Rapids


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No decisions on the backside of racetracks are black and white, and many of the most difficult decisions practitioners encounter are out of their control, one veterinarian says.
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A drinking team from the Las Vegas Valley. Originated from the depths of Lake Mead. Known to attend UNLV Rebel Basketball and Football games. Always taking applications. Required initiation is to motorboat gingers or amys.
I'm bored, I wonder what the Motorboaters are doing?
por UNLVrunrebs
11 de Agosto de 2011
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The medium- to large-sized breasts of a woman when clothed by a low cut garment that only extends superiorly as far as the widest point of her bust.
Can predispose the bearer to nipple slip.
Can predispose the bearer to nipple slip.
"So I'm just trying to work, and in she walks with these motor boaters, all cooing and jiggling, and I about f*!cking lost my mind!"
por atajack
05 de Agosto de 2009


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May 6, 2012 7:22 PM
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Considering that the Iron Patriot armor is the only one still intact at the end of IM3 this makes sense. Rhodes will likely be taking Tony's place on the Avengers, at least temporarily. He'll probably be killed or severely injured by Ultron.
Recently USA Today caught up with the Iron Patriot (aka War Machine) Don Cheadle. USA Today spoke about about the recent Golden Globe Nomination and got a great scoop on the Avengers sequel. It appears that Cheadle will be Avengers: Age of Ultron.
"Makes sense that he'd conserve his energy though, since he just wrapped the latest season of his Showtime series House of Lies, has a part to film in the next Avengers movie and then over the next few months will prepare to star in and direct a highly-anticipated Miles Davis biopic" - USA Today (seen here).
This is great news and an interesting twist on an already bloated cast. How much of the Iron Patriot, if it all, will we see in the film? Perhaps he will only be the Cheadle's alter ego, Rhodes?
Avengers: Age of Ultron arrives May 1st, 2015.
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Considering that the Iron Patriot armor is the only one still intact at the end of IM3 this makes sense. Rhodes will likely be taking Tony's place on the Avengers, at least temporarily. He'll probably be killed or severely injured by Ultron.
Ummm don't care for the Patriot, can we call him War Machine again?
redvector, I assumed the same thing at the end of IMI3. I figured that Tony Stark would just be Tony Stark and Rhodes would be The Iron Patriot. Kind of a role reversal.
redvector good point, hadn't really thought of that.
Maybe Ultron will do enough damage to IP that he ends up being the cyborg he is now
War Machine takes IM's spot in The Avengers, Jarvis gets PO'd that Stark destroys all the suits so he creates his own, Ultron kills or captures WM as an act of revenge against Stark, IM rejoins The Avengers to take down Ultron and his sentinels.
Just thinking but in Iron Man 3 the Mark suites could pretty much function by themselves. What if Tony develops some kind of A.I. software and uses the only operational suit available the Mark 2 / War Machine/ Iron Patriot and creates Ultron so being Iron Man would no longer be necessary. Probably not but just a thought.
The only person that belongs in the Iron Patriot armor is Norman Osborn. The only other suit of armor that has the same paint scheme in the iron man canon of characters is Detroit Steel.


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