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Picaud, S., Strocchia, M., Terracciano, S., Lauro, G., Mendez, J., Daniels, D.L., Riccio, R., Bifulco, G., Bruno, I. and Filippakopoulos, P.
Notes: The authors used bioluminescence resonance energy transfer (BRET) to test the ability of a bromodomain 9 ligand to disrupt binding to histone. HEK 293 cells were cotransfected with a histone H3.3-HaloTag® fusion vector and either NanoLuc®-BRD9 bromodomain or NanoLuc®-full-length BRD4 fusion vector. After 24 hours, the transfected cells were trypsinized, diluted in phenol red-free DMEM with or without 10nM of HaloTag® NanoBRET™ 618 Ligand and dispensed into a 96-well plate. One of two potential BRD-disrupting compounds, 7d or 11, was adding to a final concentration of 0.005–33μM, cells were incubated for 18 hours and NanoBRET™ Nano-Glo® Substrate (final concentration 10µM) was added. Fluorescence was measured and a corrected BRET ratio calculated. Cytotoxicity was assessed after the NanoBRET™ assay by incubating the cells with the CellTiter-Glo® Reagent for 30 minutes and measuring luminescence. To examine histone H3.3 localization, HEK 293 cells were transfected with the histone H3.3-HaloTag® fusion vector using FuGENE® HD Transfection Reagent. After 24 hours, cells were labeled with 5μM HaloTag® TMR ligand for 15 minutes before washing with complete medium, incubated for 30 minutes and imaged with a confocal microscope. (4568)
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NanoBRET™ Nano-Glo® Substrate
NanoBRET™ Nano-Glo® Detection System
HaloTag® NanoBRET™ 618 Ligand
FuGENE® HD Transfection Reagent
CellTiter-Glo® Luminescent Cell Viability Assay
HaloTag® TMR Ligand
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Mathews Griner, L.A., Guha. R., Shinn. P., Young, R.M., Keller,J.M., Liu, D., Goldlust, I.S., Yasgar, A., McKnight, C., Boxer, M.B., Duveau, D.Y., Jiang, J.K., Michael, S., Mierzwa, T., Huang, W., Walsh, M.J., Mott, B.T., Patel, P., Leister, W., Maloney, D.J., Leclair, C.A., Rai, G., Jadhav, A., Peyser, B.D., Austin, C.P., Martin, S.E., Simeonov, A., Ferrer, M., Staudt, L.M. and Thomas, C.J.
Notes: To avoid the trial-and-error methodology involved in developing multidrug treatments, the authors designed and tested a platform for high-throughput combination screening to easily discover pairs of small molecules that could then be explored further. Cell lines tested were dispensed at 1,000 cells per well in 5µl of medium into a 1,536-well plate either directly to compound matrix plates or adding 23nl of bortezomib and library compounds to the cells. After a 48-hour incubation, 3µl of CellTiter-Glo® Luminescent Cell Viability Assay reagent was dispensed and luminscence read to assess cell proliferation. (4209)
Patel, P.R., Sun, H., Li, S.Q., Shen, M., Khan, J., Thomas, C.J. and Davis, M.I.
Notes: This article describes how a miniaturized high-throughput biochemical assay for Yes1 kinase was developed and used for screening inhibitors in small molecule libraries. Kinase activity of Yes1 was assessed by dispensing 2µl of Yes1 enzyme in a 1,536-well white multiwell plate and mixing with compounds and substrate for a total kinase reaction volume of 25µl. The amount of ADP was quantitated using the ADP-Glo™ Kinase Assay and normalized to vehicle and minus-enzyme controls. Some of the inhibitors identified in the in vitro assay were tested in a cell-based assay. Two rhabdomyosarcoma cell lines, RD and RH30, were seeded at 4,000 cells/well in a 96-well plate with 100µl of culture medium. After an overnight incubation, 100µl of medium with 0, 0.1, 1, 5, 10, 15 or 20μM of inhibitor (final concentration) was added. After 48 hours, the number of cells was assessed using the CellTiter-Glo® Luminescent Cell Viability Assay. (4354)
ADP-Glo™ Kinase Assay
Sachsenmeier, K.F., Hay, C., Brand, E., Clarke, L., Rosenthal, K., Guillard, S., Rust, S., Minter, R. and Hollingsworth, R.
Notes: The authors of this paper describe the development of a high-throughput assay to screen for antibody inhbitors of 5´-ectonucleotidase activity (converts AMP to adeonsine plus free phosphate). They used the CellTiter-Glo® Cell Viability Assay to screen for activity. The CellTiter-Glo® luciferase reaction is inhibited by AMP; activity of 5´-ectonucleotidase eliminates AMP, thereby relieving the reaction from inhibition, producing light. The authors demonstrate release of the luciferase reaction from inhibition as a result of 5´-ectonucleotidase activity, and that they were able to show inhibition of 5´-ectonucleotidase activity by anti-5´-ectonucleotidase antibodies. (4261)
Qiu, R., Croom, J., Ali, R.A., Ballou, A.L., Smith, C., Ashwell, C.M., Hassan, H.M., Chiang, C.-C. and Koci, M.D.
Notes: The authors of this study investigated the energetic effects of direct fed microbials on 1-day-old broiler chicks. They looked at body weight, feed consumption, whole-body energy expenditure, organ mass, tissue respiration rates and peripheral blood mononuclear cell (PBMC) ATP levels to explore effects on energy metabolism in control-diet chicks versus chicks receiving direct fed microbials. An ATP assay was used because previous attempts to monitor O2 use by PMBCs were inconclusive. PBMC ATP levels were measured using the CellTiter-Glo™ Luminescent Cell Viability Assay in 96-well plates using 10 × 105 cells/well. The authors also looked for differences in ATP depletion in control and experimental chick populations by using an ATP synthase inhibitor and a proton ionophore. In this study, the authors concluded that the PMBC isolated from the direct microbial fed animals consumed more ATP/cell than the controls. (4202)
Thorne, C.A., Lafleur, B., Lewis, M., Hanson, A.J., Jernigan, K.K., Weaver, D.C., Huppert, K.A., Chen, T.W., Wichaidit, C., Cselenyi, C.S., Tahinci, E., Meyers, K.C., Waskow, E., Orton, D., Salic, A., Lee, L.A., Robbins, D.J., Huppert, S.S. and Lee, E.
Notes: The authors used a biochemical assay using Xenopus egg extracts to monitor degradation levels of two Wnt pathway components, Axin and ß-catenin, and identify modulators of the Wnt pathway. ß-catenin and Axin were expressed in vitro as firefly and Renilla luciferase fusion proteins, respectively, using the TNT® SP6 High-Yield Protein Expression System and shown to behave in Xenopus extracts in a similar way to wildtype proteins, which were expressed as radiolabled proteins in rabbit reticulocyte lysate. Degradation of labeled proteins was monitored by SDS polyacrylamide gel electrophoresis and autoradiography, and degradation of luciferase fusion proteins was examined using the Dual-Glo® Luciferase Assay. Using the Xenopus extract-based assay, the authors screened chemical libraries to identify two modulators of the Wnt pathway, then confirmed this inhibition in HEK 293 cells by demonstrating a decrease in ß-catenin expression with increasing concentrations of the inhibitors. This decrease in ß-catenin-Fluc levels was measured using the Steady-Glo™ Luciferase Assay, and luciferase activity was normalized to cell viability, as determined using the CellTiter-Glo® Assay. (4181)
Dual-Glo® Luciferase Assay System
Rabbit Reticulocyte Lysate System, Nuclease Treated
Steady-Glo® Luciferase Assay System
TnT® SP6 High-Yield Wheat Germ Protein Expression System
Moeller, T.A., Shukla, S.J. and Xia, M.
Notes: Here the authors describe development of an HTS cell viability assay protocol for use with cultured cyropreserved human primary hepatocytes. Cryopreserved hepatocytes for culturing were prepared as suspensions and dispensed at 2,000 or 4,000 cells/5µl/well in collagen I-coated 1,536-well plates. Cells were allowed to attach and then 23nl of each test compound was added in a dilution series from 2.8nM to 92µM, and cells incubated for 24 or 40 hours. Five microliters of CellTiter-Glo® Reagent was added and cells were incubated 30 minutes before reading the luminescent output. IC50 values for 12 compounds were determined; a summary of the protocol is provided in Table 1 of the paper. Cultured cryopreserved hepatocytes were assayed for function using the P450-Glo® CYP3A4 assay with the Luciferin-IPA substrate. (4182)
CellTiter-Fluor™ Cell Viability Assay
P450-Glo™ CYP3A4 Assay with Luciferin-IPA
Zielinski, R., Lyakhov, I., Hassan, M., Kuban, M., Shafer-Weaver, K., Gandjbakhche, A. and Capala, J.
Notes: The authors used the CellTiter®-Glo Assay to assess the effect of HER2-Affitoxin on cell viability of gastric carcinoma NCI-N87 cells, which overexpress HER2. The HER2-Affitoxin is a fusion protein of a HER2-specific Affibody and modified Pseudomonas aeruginosa exotoxin A (PE 38). This fusion protein was designed to offer an alternative method for treating tumors that do not respond to trastuzumab or have acquired resistance to current therapies. The CellTiter-Glo® Assay was used to calculate HER2-Affitoxin IC50 values of cells in culture. They describe the mechanism of action as follows: HER2-Affitoxin binds to HER2 protein on the cell surface, is internalized, where it blocks protein synthesis in the cytosol via ADP ribosylation of eEF-2. (4131)
Ikner, A. and Ashkenazi, A.
Notes: The authors of this study set out to describe the mechanism of cell death through which TNF-like weak inducer of apoptosis (TWEAK) exerts its apoptotic effect on certain cancer cells. The used the CellTiter-Glo® Cell Viability Assay and the the Caspase-Glo® 3/7 Assay to investigate cell viability and mechanism of cell death in HSC3 cells treated with TWEAK. They looked at caspase-8 activity in cells treated with TWEAK in the presence or absence of a caspase-8 inhibitor using the Caspase-Glo® 8 Assay. They showed that TWEAK induces caspase-dependent apoptosis in these cells. (4170)
Caspase-Glo® 3/7 Assay
Caspase-Glo® 8 Assay
Miller SC, Huang R, Sakamuru S, Shukla SJ, Attene-Ramos MS, Shinn P, Van Leer D, Leister W, Austin CP, Xia M.
Notes: Dysregulation of the NF-kB pathway has been associated with the formation of a wide variety of tumors and other cancers, as well as diseases, including chronic inflammation and immunodeficiency. Because of the association of constitutive NF-kB regulation and tumors, inhibition of the NF-kB pathway by small molecule antagonists was thought to be a means of reversing or halting the growth and spread of tumors. The authors screened compounds from a database (the NCGC Pharmaceutical Collection or NPC) of small molecule compounds: 52% of the compounds have been approved for human or animal use by the FDA, 22% were drugs approved for use in Europe, and another 25% either drugs approved in other countries or compounds that have been tested in clinical trials. The database served as a source from which to rapidly and efficiently identify already approved drugs that inhibited NF-kB. They used a quantitative high-throughput screening format. To identify small molecules that could inhibit the NF-kB pathway, the compounds were initially screened using a cell-based NF-kB lactamase reporter gene assay, with TNFalpha and MG132 as positive controls. (TNFalpha induced NF-kB coupled beta-lactamase activity, while MG132 blocked TNFalpha induction NF-kB-coupled beta-lactamase activity.) After several rounds of screening, 20 compounds were further studied for their NF-kB inhibition, with NF-kB luc2P HEK293 cells. After a concordance rate of 95% between the luciferase and beta-lactamase tests, compounds were additionally examined for their ability to affect caspase 3/7 activity, for the ability to disrupt the electrochemical gradient across the mitochondrial membrane in relation to cellular apoptosis, as well as tests of the inhibitors on cancer cell viability and affects on LDH release, an indicator of cell necrosis. (4049)
CytoTox-Glo™ Cytotoxicity Assay
GloResponse™ NF-κB-RE-luc2P HEK293 Cell Line
Zhang, L.H., Kamanna, V.S., Ganji, S.H., Xiong, X-M. and Kashyap, M.L.
Notes: The authors investigated the role of pioglitazone on transcriptional regulation of the apoA-I gene and looked at the biological properties of pioglitazone-induced apoA-I-containing high-density lipoprotein particles (HDL). To investigate the biological properties of the HDL particles, the authors treated THP-1 cells with conditioned medium from HepG2 cultures treated or untreated with pioglitazone and looked at adhesion to human aortic endothelial cells (HAEC). During the experiment, HAEC viability and proliferation were monitored using the CellTiter-Glo® Luminescent Cell Viability Assay. Additionally, to determine whether pioglitazone stimulates apoA-I transcription, a luciferase reporter construct was made containing the apoA-I gene promoter. Transfected cells were treated with pioglitazone, and luciferase expression was monitored using the Dual-Luciferase® Reporter Assay System. (4173)
pGL3-Basic Vector
Gupta, P.B., Onder, T.T., Jiang, G., Tao, K., Kuperwasser, C., Weinberg, R.A. and Lander, E.S.
Notes: The authors of this study describe a proof-of-concept screen to use mammary epithelial cells that have been induced to undergo an epithelial to mesenchymal transition (EMT) as model cells to identify agents that may be selectively toxic against "epithelial cancer stem cells" (CSCs). They induced the transformed breast cancer cell line HMLER to undergo a mesenchymal transition using shRNA directed against the E-cadherin gene. They characterized the responsiveness of these transitioned cells to common cytotoxic agents using the CellTiter® 96 AQueous Cytoxicity Assay and compared the response to that of HMLER cells containing a control shRNA. They showed that the HMLER cells induced to undergo EMT behaved more like CSCs. The researchers then performed a proof-of-concept high-throughput screen to identify compounds that targeted the HMLER cells induced to undergo EMT, using the CellTiter-Glo® Assay to assess cell viability. (4006)
CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay
Niles, A.L., Moravec, R.A. and Riss, T.L.
Notes: The authors review the use of in vitro cytotoxicity testing in drug discovery to characterize the toxic potential of new chemical entities (nce) at the earliest stages of profiling. DOI: 10.2174/1875397300903010033 (4000)
Apo-ONE® Homogeneous Caspase-3/7 Assay
Apo-ONE® Homogeneous Caspase-3/7 Buffer
CellTiter-Blue® Cell Viability Assay
MultiTox-Fluor Multiplex Cytotoxicity Assay
MultiTox-Glo Multiplex Cytotoxicity Assay
Wu, Y., Connors, D., Barber, L., Jayachandra, S., Hanumegowda, U.M. and Adams, S.P.
Notes: The authors describe a multiplexed in vitro assay to detect nephrotoxicity and gain information about mechanism of cell death in HK-2 (human kidney-2) cells. The multiplexed assay involved an LDH assay to detect necrosis, a caspase-3/7 assay to detect apoptosis, a reazurin assay to assess metabolic state, and a DNA dye staining assay to monitor nuclear morphology. (4002)
CytoTox-ONE™ Homogeneous Membrane Integrity Assay
CytoTox-ONE™ Homogeneous Membrane Integrity Assay, HTP
Zheng, Y., Ritzenthaler, J.D., Sun, X., Roman, J. and Han, S.
Notes: In this paper, the role of prostaglandin E2 (PGE2) stimulation of integrin-linked kinase (ILK) in human lung carcinoma was explored. Mutations of Sp1 and NF-κB cis-acting elements in an ILK promoter-pGL3-Basic Vector construct were created using the GeneEditor™ in vitro Site-Directed Mutagenesis System. The mutations were confirmed via sequencing. Human non–small cell lung carcinoma (NSCLC) cells were plated at a density of 5 × 105 cells per well in six-well plates and transfected with 2µg of ILK promoter reporter vectors with or without 0.2µg of the phRL-TK Renilla Luciferase Reporter Vector. After 24 hours, the transfected cells were exposed to PGE2 and the cells lysed for assessment using the Dual-Luciferase® Reporter Assay System. NSCLC cells were transfected with inactive (ILK-S343A) and superactive ILK (ILK-S343D) cDNA, incubated for 24 hours, treated with or without exogenous PGE2 or with an Sp1 inhibitor for 2 hours. The numbers of viable cells were measured using the CellTiter-Glo® Luminescent Cell Viability Assay. (4026)
Cali, J.J., Niles, A., Valley, M.P., O'Brien, M.A., Riss, T.L., and Shultz, J.
Notes: The authors of this review article highlight the use of bioluminescence as a readout for high-throughput ADME/Tox assays. They discuss three strategies for designing bioluminescent assays, using either luciferase, ATP or luciferin substrates as the limiting reagents for a luciferase-catalyzed reaction. Reporter gene assays limit the production of luciferase by tying it to a promoter or DNA regulatory region of interest. Such assays can be used to study genes that are regulated by drugs and other xenobiotics. Bioluminescent assays in which ATP is the limiting reagent of the luciferase reaction can be designed to monitor cell viability or the activity kinases. Bioluminescent assays in which the substrate is limiting can be designed so that the activity of a particular enzyme results in the production of a luciferin substrate that can, in turn, be acted upon by luciferase. (3926)
Beta-Glo® Assay System
Calpain-Glo™ Protease Assay
Caspase-Glo® 2 Assay
Caspase-Glo® 6 Assay
Caspase-Glo® 9 Assay
GloResponse™ CRE-luc2P HEK293 Cell Line
GloResponse™ NFAT-RE-luc2P HEK293 Cell Line
GSH-Glo™ Glutathione Assay
Kinase-Glo® Luminescent Kinase Assay
Kinase-Glo® Max Luminescent Kinase Assay
Kinase-Glo® Plus Luminescent Kinase Assay
Luciferase Assay Reagent
Luciferase Assay System
MAO-Glo™ Assay
P450-Glo™ CYP1A1 Assay
P450-Glo™ CYP1A2 Assay
P450-Glo™ CYP1A2 Screening System
P450-Glo™ CYP1B1 Assay
P450-Glo™ CYP2C19 Assay
P450-Glo™ CYP2C8 Assay
P450-Glo™ CYP2C9 Assay
P450-Glo™ CYP2D6 Assay
P450-Glo™ CYP3A4 Assay
P450-Glo™ CYP3A4 Assay (Luciferin-PFBE)Cell-Based/Biochemical Assay
P450-Glo™ CYP3A4 Assay (Luciferin-PPXE)DMSO-Tolerant Assay
P450-Glo™ CYP3A7 Assay
Proteasome-Glo™ 3-Substrate System
Proteasome-Glo™ Caspase-Like Assay
Proteasome-Glo™ Chymotrypsin-Like Assay
Proteasome-Glo™ Chymotrypsin-Like Cell-Based Assay
Proteasome-Glo™ Trypsin-Like Assay
Lin, H., Lee, E., Hestir, K., Leo, C., Huang, M., Bosch, E., Halenbeck, R., Wu, G., Zhou, A., Behrens, D., Hollenboguh, D., Linnemann, T., Qin, M., Wong, J., Chu, K., Doberstein, S.K. and Williams, L.T.
Notes: The authors of this study created a cDNA library representative of the extracellular proteome (secreted proteins and the extracellular domains of transmembrane proteins). Each cDNA was individually transfected into 293T cells. Medium from the cDNA of each transfection was used in a suite of cell-based assays. The CellTiter-Glo® Assay was used to screen for secreted factors from the cell lines expressing the cDNA that affected viability of twelve cell lines: human primary B cells, human primary T cells, human primary NK cells, human primary monocytes, A549 cells, Colo205 cells, U-118 cells, MDA-MB231 cells, PC3 cells, PANC1 cells, human primary skeletal muscle progenitor cells, and rat primary oligodendrocyte precursor cells. (3935)
Hahn, C.K., Ross, K.N., Warrington, I.M., Mazitschek, R., Kanegai, C.M., Wright, R.D., Kung, A.L., Golub, T.R. and Stegmaier, K.
Notes: The authors designed a high-throughput gene-expression screen to identify compounds that induce a neuroblastoma gene signature in BE(2)-C cells. The screen used Valproic Acid (VPA), an inhibitor of histone deacetylase, as an enhancer. The top hit from the screen was all-trans retinoic acid (ATRA). The authors proposed that ATRA + VPA would reduce cell viability and might induce apoptosis by inducing genes associated with terminal differentiation. They used the CellTiter-Glo® Luminescent Cell Viability Assay in a 96-well format to assess the effects of ATRA and a variety of inhibitors of histone deacetylase on BE(2)-C cell viability. (3931)
Liu, Y., Kern, J.T., Walker, J.R., Johnson, J.A., Schultz, P.G., and Luesch, H.
Notes: These authors screened a library of 15,000 expression cDNAs in neuroblastoma IMR-32 cells searching for genes that activate the antioxidant response element, ARE. ARE is a cis-acting enhancer element found in the 5´ flanking region of many genes that are involved in protection from oxidative stress. The library was screened using a luciferase reporter construct under the control of an ARE-containing promoter. Luminescence, indicating the presence of cDNA-activating ARE, was measured using the Bright-Glo™ Luciferase Assay System. cDNA clones showing reproducible activation were selected for further analysis. The authors tested the effect of over expression of these ARE activators on the ability to resist oxidative stress. IMR-32 cells expressing the various cDNAs were exposed to hydrogen peroxide or rotenone, and the effect on cell viability was measured using the CellTiter-Glo® Assay. Cells overexpressing the ARE-activators were more resistant to oxidative stress than controls. (3629)
Bright-Glo™ Luciferase Assay System
Prichard, M.N., Daily, S.L., Jefferson, G.M., Perry, A.L. and Kern, E.R.
Notes: The authors developed an assay to evaluate antiviral compounds for Epstein-Barr virus (EBV) infections. EBV DNA was isolated using the Wizard® SV 96 Genomic DNA Purification System, and 5µl was used for real-time PCR to quantify the viral DNA. Cytotoxicity of the antiviral compounds was assessed using treated, uninfected Akata cells compared to those with no treatment or infection. After three days when the viral DNA was harvested, cell viability was measured using the CellTiter-Glo® Luminescent Cell Viability Assay. (3761)
Wizard® SV 96 Genomic DNA Purification System
Lynch, R.A., Etchin, J., Battle, T.E. and Frank, D.A.
Notes: STAT1 is a transcription factor that is involved in a variety of cellular processes and behaves like a tumor suppressor in many ways. It is associated with apoptosis, inhibition of cyclin-dependent kinases, and it may mediate the antitumor effects of IFN-γ. The authors of this study designed a bioluminescent reporter assay to identify small molecules that enhance STAT1-dependent gene expression. The bioluminescent assay was performed in 384-well plates using both stably and transiently transfected cell lines. The screening assay was robust, producing a Z´factor value of 0.92, and it identified three compounds that specifically enhanced STAT1-dependent transcriptional activity. Firefly luciferase activity in stable cell lines was assessed using the Bright-Glo® Luciferase Assay System; luciferase activity in the transiently transfected cells was monitored using the Dual-Luciferase® Assay System. Viability of cells in culture was monitored using the CellTiter-Glo® Assay. (3732)
Dual-Luciferase® Reporter Assay System
pRL-TK Vector
Chen, E.I., Hewel, J., Kreuger, J.S., Tiraby, C., Weber, M.R., Kralli, A., Becker, K., Yates, J.R., and Felding-Habermann, B.
Notes: This study investigated protein expression profiles in tumors from breast cancer brain metastases. Circulating tumor cells were isolated from a patient with stage IV breast cancer and injected into SCID mice. Tumor cells were then recovered from brain and bone lesions that subsequently developed in these mice. The protein expression profiles of the parent cell line were then compared with those from brain and bone tumors. More than 300 proteins that were up- or down-regulated in the brain tumor cells were identified. Classification of these proteins by function revealed that the majority were associated with cellular metabolism. Sixty-three differentially expressed proteins, including mainly cellular redox-active proteins and proteins involved in glucose and fatty acid oxidation, were selected for further study. Based on the expression data, a metabolic profile of brain metastatic cells was generated. Up-regulation of genes involved in oxidative energy metabolism as indicated by the proteomic analysis was confirmed by quantitative real-time PCR analysis. Consistent with the observation of increased glycolysis and oxidative phosphorylation, the authors also found that levels of cellular ATP were increased in cells from brain metastases. The CellTiter-Glo® Luminescent Cell Viability Assay was used to measure ATP levels in the primary, bone, and brain-derived tumor cells. The authors suggest that adaptation of the tumor cell energy metabolism is a key development in breast cancer brain metastasis. (3613)
Fan, F. and Wood, K.V.
Notes: The authors of this paper review bioluminescent assay technologies, discussing HTS reporter, cell-based and luciferase biosensor assays. They divide luminescent assays into three basic categories: assays that measure ATP concentration (cell viability and kinase assays), assays that measure changes in luciferase levels (reporter assays, GPCR assays), and assays that measure changes in luciferin levels (protease [including caspase], P450 and MAO assays). (3737)
BacTiter-Glo™ Microbial Cell Viability Assay
cAMP-Glo™ Assay
Dual-Luciferase® Reporter 1000 Assay System
Dual-Luciferase® Reporter Assay System 10-Pack
P450-Glo™ CYP2C19 Screening System
P450-Glo™ CYP2C9 Screening System
P450-Glo™ CYP2D6 Screening System
P450-Glo™ CYP3A4 Screening System
P450-Glo™ CYP3A4 Screening System (Luciferin-PPXE)DMSO-Tolerant Assay
Renilla Luciferase Assay System
Severson, W.E., Shindo, N., Sosa, M., Fletcher, T., White, L., Ananthan, S. and Jonsson, C.B.
Notes: The authors of this paper describe the validation of a bioluminescent assay using the CellTiter-Glo® Reagent to identify novel compounds that inhibit the cytopathic effect of SARS CoV. In this study, three cell viability assay reagents were evaluated: MTS, neutral red, and CellTiter-Glo® Reagent. The CellTiter-Glo®-based assay was chosen because it did not require washing or medium removal; it involved minimal pipetting steps and had a short incubation time, which reduced time in the BSL3 containment facility. The assay was used to screen 100,000 compounds and identified several compounds that inhibited CPE while having a minimal effect on cell viability. (3736)
Galkin, A.V., Melnick, J.S., Kim, S., Hood, T.L., Li, N., Li, L., Xia, G., Steensma, R., Chopiuk, G., Jiang, J., Wan, Y., Ding, P., Liu, Y., Sun, F., Schultz, P.G., Gray, N.S. and Warmuth, M.
Notes: NVP-TAE684 was identified as an inhibitor of the constitutive anaplastic lymphoma kinase (ALK) activity associated with the NPM-ALK fusion. NPM-ALK fusion protein is created by translocation event characteristic of anaplastic large-cell lymophomas. NVP-TAE684 was screened against a panel of 35 Ba/F3 cell lines expressing a variety of tyrosine kinases constitutively activated by fusion to TEL. The CellTiter-Glo® Luminescent Cell Viability Assay was used to detect any decrease in viability as a result of treatment with NVP-TAE684. The inhibitory effect of NVP-TAE684 was specific for ALK-associated cell proliferation. In a secondary screen to determine potency, TAE684 was screened against two human anaplastic large-cell lymphoma cell lines. Inhibition of proliferation correlated with dosage and was assessed using the Bright-Glo® Luciferase Assay System. (3738)
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Debugging, the demanding process of finding and fixing programming errors, is fundamental to successful software development. But even many experienced programmers find debugging a challenge.
The Art of Debugging with GDB, DDD, and Eclipse from No Starch Press teaches readers how to effectively use the three most popular open source debugging tools: GDB, DDD, and Eclipse.
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Great Bend High School embarks on Western Athletic Conference-opening play tonight, when the Panthers travel out west to take on Garden City.
CONCORDIA - Cloud County Community College withstood every challenge Barton's women made at them Wednesday night at Arley A. Bryant Gymnasium.
CONCORDIA - The Kansas Jayhawk Community College Conference Western Division opener between Cloud County Community College and Barton was a matter of survival.
McPHERSON - The Great Bend High School girls' basketball team had its moments while taking on Class 5A No. 7-ranked McPherson Tuesday night at The Roundhouse.
McPHERSON - The inside-outside combination featuring 6-foot-6 post player Christian Ulsaker and point guard Jack Pyle makes Class 5A top-ranked McPherson a tough matchup.
McPHERSON - Great Bend High School girls' head basketball coach Jason Tatkenhorst genuinely believes his team made some positive gains during practices over the Christmas holidays.
McPHERSON - The prospect of playing in The Roundhouse at McPherson High School is always an interesting sidelight.
MANHATTAN - Kansas State forwards Wally Judge and Jamar Samuels have been a steadying influence while teammates Jacob Pullen and Curtis Kelly have been sidelined by the NCAA for committing minor infractions.
The buzz surrounding the controversial unsportsmanlike penalty at the end of the inaugural Pinstripe Bowl was a focus of the national sports scene on Friday.
MANHATTAN - At least for one night, Kansas State could forget about the controversy surrounding the suspensions of seniors Jacob Pullen and Curtis Kelly.
MANHATTAN - In the aftermath of an 80-64 win over Missouri-Kansas City Thursday night at Bramlage Coliseum, Kansas State head basketball coach Frank Martin broke his silence about the suspensions of seniors Jacob Pullen and Curtis Kelly.
More details have emerged regarding the suspensions of Kansas State's Jacob Pullen and Curtis Kelly.
KANSAS CITY, Mo. - Guard Oscar Bellfield chuckled with UNLV teammate Tre'Von Willis during the postgame conference with the media.
KANSAS CITY, Mo. - In his college basketball debut on Saturday at storied Allen Fieldhouse, Kansas guard Josh Selby showed that he can make an immediate impact.


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Andre Iguodala and the Nuggets were beaten by the Warriors during the playoffs, but now he joins Stephen Curry in Oakland. / Kyle Terada, USA TODAY Sports
The Golden State Warriors landed free agent small forward Andre Iguodala on a four-year, $48 million deal, two people with knowledge of the agreement told USA TODAY Sports.
In order to clear the necessary cap space for the move, the Warriors agreed on a trade that will send Richard Jefferson, Andris Biedrins and Brandon Rush to the Jazz. Golden State gets guard Kevin Murphy while giving the Jazz first-round picks in 2014 and 2017 as well as two second-round picks. The people spoke on the condition of anonymity because the deal had not yet been announced.
Once Iguodala showed a serious interest in joining the Warriors, they attempted to engage his former team, the Denver Nuggets, in a possible sign-and-trade deal but were rebuffed. That left them with only the option of clearing space to bring him to town.
Yet while they aggressively pursued the salary relief that comes with unloading the combined $24 million in expiring contracts from Biedrins, Jefferson and Rush, the trade discussions were widely assumed to have been a way to land free agent center Dwight Howard. But Howard elected to join the Houston Rockets on Friday.
Copyright 2015
Read the original story: Warriors land Andre Iguodala after trade with Jazz


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Stop aging in its tracks: Beauty tips to try today
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RC Nightmare Community Blog
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Wow! You can win this (famous) Traxxas Ken Block Edition. The give away ends on the last day of June here in 2011, and you have to pay nothing at all to enter!
Win This Exact RC:
Here are the rules:
We are running a point total for all our fans on youtube. There are a few ways to earn an entry, there is NO limit to the number of entries you can gain, and the more points you pile up the better chance you have to WIN!.
All You have to do is refer people to subscribe to our youtube channel. You get one entry for everyone you refer, if they subscribe to both our channels RC NightMare and Friend Of RC you will score two points.
Then once they sign up, you will score a point for each person they refer.
Now if you are someone who has been referred you need only let us know by commenting below WHO referred you and you will get a free entry. Now that you are referred you can start sending people and getting points as well.
We will be posting the ongoing results below!
Traxxas Spartan Maiden Voyage-No OverHeat
Yes Kiddies! We have finally taken our brand new Traxxas Spartan out for its first run. If only I wasn't such an idiot and I waited until now to buy it instead of rushing out in February to score it and then wait 3 months lol. Many people have been hating on the Traxxas Spartan for overheating and I am here to say we had no issues of the type yesterday. We were only running 5 Cell Lipo's but we ran for a good amount of time and let me tell you this, The Traxxas Spartan RC boat is every bit as much fun as you might think it to be. In terms of filming we will have to get a little more creative as who wants to watch 3 minutes of us zooming back and forth but as you know RC NightMare has got something up our sleeves to make it looks amazing.
I was afraid first of all that the boat would be hard to handle and I might flip it, then have to swim my fat butt out in the cold water to go get it, however that just didn't happen. I will say the turning radius left something to be desired but that is likely a big contributing factor to the fact I did not flip it, had I had the ability to turn more sharply I am sure I would of and I am sure I would of been more likely to roll the thing.
That said, we have another run scheduled for later this week, and hopefully we can test out 6S power for you guys to confirm our Rudder fix worked in terms of cooling, I have also been kicking around the idea of just going ahead and adding another cooling line to the Traxxas Spartan just for good measure and might have Brandon help me out with that before the next run.
That said the Traxxas Spartan RC Boat was super fun, super fast and worth the wait for sure. As we compile the footage for a running video, here are some pictures Cory took yesterday.


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Avocado Pro™ (C7687)
Avocado Pro™ opens, pits, slices, scoops and mashes! This 5-in-1 tool lets you prepare avocadoes as a garnish, for a salad or guacamole. Use the serrated edge to open the avocado and to remove the pit. Then drag the slicer through the meat for even wedges. Use the opposite end to mash. It's the easy way to enjoy prepare this healthy food! 9 ½" L. Top rack dishwasher safe.
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"Star Wars: Episode VII" director J.J. Abrams tweets a photo about the Apple Watch, but fans are more interested in the background image, which looks a lot like the inside of the Death Star.
The director offers a peek at the X-wing fighter from the set of "Star Wars Episode VII" to promote the Force for Change campaign that raises money for UNICEF.
In a pleading note, or perhaps a coy marketing device, the director asks that people stop leaking photos, especially of the Millennium Falcon, from the "Star Wars: Episode VII" set.
The Sincerely Truman ad agency offers up four simple rules for J.J. Abrams to follow to make "Star Wars" special once again.
Robert Sallin, producer of "Star Trek II: The Wrath of Khan," shares his experience working on the film, looks at the future of "Trek," and dishes on whether that was Ricardo Montalban's real chest.
The social network is live-streaming a conversation with the director, along with original Mr. Sulu George Takei, Friday evening, by way of its Facebook Live site.
Many fans think a "Star Wars" film just wouldn't feel right without John Williams' signature symphonic sound. Director J.J. Abrams seems to agree.
Captain Kirk weighs in on J.J. Abrams taking on the new "Star Wars" movie, and he doesn't sound too happy about it.
The Hollywood Reporter details Lucasfilm boss Kathleen Kennedy's cajoling of the "Star Trek" director and drops a bomb: The next "Star Wars" may not be ready by 2015.
After speculation leaked out of Hollywood yesterday, it's official: One man will be in charge of both "Star Trek" and "Star Wars" at the same time.


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(Thanks to the Jingle Cats)
More Jingle Cats here.
Friday, 21 December 2007
1 comment:
Merry Christmas!
That video is probably going to give me nightmares, though! - Friday, 21 December 2007 at 10:19:00 GMT
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Passionate about IP! Since June 2003 the IPKat weblog has covered copyright, patent, trade mark, info-tech and privacy/confidentiality issues from a mainly UK and European perspective. The team is David Brophy, Birgit Clark, Merpel, Jeremy Phillips, Eleonora Rosati, Darren Smyth, Annsley Merelle Ward and Neil J. Wilkof. You're welcome to read, post comments and participate in our community. You can email the Kats here
Merry Christmas!
That video is probably going to give me nightmares, though!
* Recommended by the European Patent Office as reading material for candidates for the European Qualifying Examinations 2013
* Listed as a "Top Legal Blog" in The Times Online, March 2011
* One of only two non-US weblogs listed in the 2010 ABA Journal Blawg 100
* Court Reporter Top Copyright Blog award winner, November 2010
* Number 1 in the 2010 Top Copyright Blog list compiled by the Copyright Litigation Blog, July 2010
* Selected by United States Library of Congress for inclusion in its historic collections of Internet materials related to Legal Blawgs 2010
* Top Patent Blog poll 2009: 3rd out of 50 in the "Favourite Patent Blog" poll and 2nd out of 50 in the "Most-read" poll
* ComputerWeekly IT Law and Governance Blog of the Year 20 August 2008
* Best of the Blogs, Times Online, 21 August 2008
* Listed as one of Managing Intellectual Property magazine's Fifty Most Influential People of 2005, 2011, 2013 and 2014


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Dr. Stjerna Takes New Call at PLTS
Kirsi Stjerna to serve as First Lutheran, Los Angeles/Southwest California Synod Professor at PLTS read more
Tenor and Guitar in Concert
Tenor Jeffrey Fahnestock and Guitarist James Hontz Warm the House for Music, Gettysburg! read more
For Vigorous and Prophetic Action on Social and Racial Injustice
New MDivForward curriculum begins in 2015 read more
MDivForward: God's call. Your path.
New MDivForward curriculum begins in 2015 read more


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Posted on 6/13/2014 by Janis Fox
Want to write a book? Already written one? How can you take it to the next level and get a book actually published by a major publisher?
Former young adult book editor Sarah Jae-Jones will explain the process from an insider's perspective at Teen Central (at the Central Library). She'll discuss what it takes to get a book noticed and published by the major publishing companies. The focus is on young adult novels, but she will also share generalized information.
Saturday, June 28th at 1 p.m.
For more information, please call Teen Central at 703-3080. This program is free and open to the public, both teens and adults.
3185 Buchanan Street Winston-Salem, NC 27127 (336) 703-2980 (336) 771-4724 FAX
1:00 - 5:00 Sunday 10:00 - 9:00 Monday 10:00 - 9:00 Tuesday 10:00 - 9:00 Wednesday 10:00 - 6:00 Thursday 10:00 - 6:00 Friday 10:00 - 5:00 Saturday Hours for All Locations Holiday Closings


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I had a question regarding San Marzano tomatoes and the DOP designation.Some have posted that the La Bella brand of San Marzano should be avoided. While I understand they are not marked DOP, does anyone know whether these are from the San Marzano region of Italy. I have used this brand several times and they have been outstanding - they are sweet, they tomatoes have all been high quality and fully ripened, and you could eat them straight out of the can they are so good. The Pennsylvania Macaroni Company carries them also, so I thought that gave them some credibility. I will be traveling to New Jersey in July and planned to visit the distributor listed on the web.ThanksTom
I will be going to New Jersey in July to visit my parents, they live about 15 minutes away from the La Bella San Marzano importer, I can go over and knock on their door - apparently they sell out of a garage adjacent to their house from what someone posted


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A heart attack is a medical emergency. Call 911 or your local emergency number if you think you or someone else is having a heart attack.
The average person waits 3 hours before seeking help for symptoms of a heart attack. Many heart attack patients die before they reach a hospital. The sooner the person gets to the emergency room, the better the chance of survival. Prompt medical treatment reduces the amount of heart damage.
This article discusses what to do if you think someone may be having a heart attack.
For more information on the condition itself, see: Heart attack.
First aid - heart attack; First aid - cardiopulmonary arrest; First aid - cardiac arrest
A heart attack occurs when the blood flow that carries oxygen to the heart is blocked. The heart muscle becomes starved for oxygen and begins to die. See: Heart attack
Symptoms of a heart attack can vary from person to person. They may be mild or severe. Women, the elderly, and people with diabetes are more likely to have subtle or unusual symptoms.
Symptoms in adults may include:
Call 911 or your local emergency number immediately if the person:
Adults should take steps to control heart disease risk factors whenever possible.
Hollander JE. Acute coronary syndromes. Acute myocardial infarction and unstable angina. In: Tintinalli JE, Kelen GD, Stapczynski JS, Ma OJ, Cline DM, eds. Emergency Medicine: A Comprehensive Study Guide. 6th ed. New York, NY: McGraw-Hill; 2004:chap 50.
Anderson JL, Adams CD, Antman EM, Bridges CR, Califf RM, Casey DE Jr., et al. ACC/AHA 2007 guidelines for the management of patients with unstable angina/non-ST-elevation myocardial infarction: a report of the American College of Cardiology/American Heart Association Task Force on Practice Guidelines (Writing Committee to Revise the 2002 Guidelines for the Management of Patients with Unstable Angina/Non-ST-Elevation Myocardial Infarction) developed in collaboration with the American College of Emergency Physicians, the Society for Cardiovascular Angiography and Interventions, and the Society of Thoracic Surgeons endorsed by the American Association of Cardiovascular and Pulmonary Rehabilitation and the Society for Academic Emergency Medicine. J Am Coll Cardiol. 2007;50:e1-e157.
Brady WJ, Harrigan RA, Chan TC. Acute coronary syndrome. In: Marx JA, Hockberger RS, Walls RM, et al, eds. Rosen's Emergency Medicine: Concepts and Clinical Practice. 7th ed. Philadelphia, Pa: Mosby Elsevier; 2009:chap 76.
In: Marx JA, Hockberger RS, Walls RM, et al, eds. Rosen's Emergency Medicine: Concepts and Clinical Practice. 7th ed. Philadelphia, Pa: Mosby Elsevier; 2009.
A.D.A.M., Inc. is accredited by URAC, also known as the American Accreditation HealthCare Commission (www.urac.org). URAC's accreditation program is an independent audit to verify that A.D.A.M. follows rigorous standards of quality and accountability. A.D.A.M. is among the first to achieve this important distinction for online health information and services. Learn more about A.D.A.M.'s editorial policy, editorial process and privacy policy. A.D.A.M. is also a founding member of Hi-Ethics and subscribes to the principles of the Health on the Net Foundation (www.hon.ch) The information provided herein should not be used during any medical emergency or for the diagnosis or treatment of any medical condition. A licensed medical professional should be consulted for diagnosis and treatment of any and all medical conditions. Call 911 for all medical emergencies. Links to other sites are provided for information only -- they do not constitute endorsements of those other sites. © 1997- 2013 A.D.A.M., Inc. Any duplication or distribution of the information contained herein is strictly prohibited.
This page was last updated: May 20, 2014


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Product Details
A clean-cut standard that goes with just about anything, the polo has been a men's wardrobe staple for years. We constructed ours from supersoft cotton to handle the nine-to-five and the five-to-nine with ease and gave it a twist by accenting it with repp-stripe detailing at the interior neck and vent linings. Online only.


text: This is most I've read about the storyline for this movie yet. Gotta say, sounds kind of lame. I thought the first one was more RedBox than Theaterworthy and this one looks the same, or more probably, worse. Even for the tone of these movies, I'm betting Carrey goes over the top. I think they waited too long between sequels too, the main actors are 3 years older which is a lifetime in the evolution of a teenager. I know I post here a lot saying most new movies look questionable or worse, so maybe I'm a cynic or a pessimist, but for what it's worth, I usually hope that I'm wrong. Usually; if Battleship had been good, I would have been pissed.


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Having been a Marine for a little over three years, and having already served in Iraq, Marshall Bowen has been assigned back to Iraq. But, Bowen will not be returning to Iraq as a rifleman, "a basic grunt in the Marine Corps," but as a Corporal in the 3rd Battalion, 1st Marine Division, Scout Sniper Platoon. Bowen, son of Ky and Barb Bowen of Philip, originally joined the military because, "I wanted to see something different, to see what else is out there." While in the Marines, he has seen areas of Africa and Europe, especially Spain.
The Philip Volleyball Tournament on Saturday, September 3, included 10 teams. After a long day of play, 9:00 a.m. to 9:00 p.m., Philip earned fourth place. Two pools of competition determined the top four teams. Bennett County taking the championship by defeating Belle Fourche 25-20, 19-25, 21-25. The consolation match determined third place when Lead defeated Philip 18-25, 25-11, 25-18. The two finals matches had Bennett County defeating Philip 28-26, 25-5; and Belle Fourche defeating Lead 25-22, 25-16.Pool AJC/Mid. over Kadoka 25-17, 15-25, 25-20.Bennett Co.
The Philip volleyball team defeated both the Lyman County and the Stanley County teams during the Philip three-way meet on Thursday, September 8. The Scotties needed to play only four games to win three out of five to triumph over Lyman County – 25-21, 25-22,17-25, 25-23. The Lady Scotties made even quicker work of Stanley County, winning three games in a row – 25-21, 25-20, 25-18.Philip vs. Lyman Co. Serving: 88 of 92 (4 aces). Leaders: Slovek - 20 of 20 (1 ace); Holman - 13 of 13 (1 ace); Fitch - 17 of 19 (1 ace). Receiving: 45 of 53.
On Saturday, September 10, the Scotties raced for the second time in three days, this time on the Kadoka Airport. The meet's nickname of "Cactus Central" was present in conversations afterward concerning runners having to pull cactus spines out of their shoes during and after the race. This and the weather did not stop the 140 runners from Kadoka and 10 neighboring schools from competing. Holly Iwan was Philip's single elementary runner out of 50 in her division's race.
On Thursday, September 8, the Philip Scottie cross country team raced on the Hillsview Golf Course in the Pierre Invitational Meet. The flat, winding course was run by 148 athletes representing 15 schools in four divisions. With the temperature in triple digits, and the humidity high with the nearness of the Missouri River, the race proved challenging for the Scotties. The junior varsity girls, Emily Schofield and Jennifer Stangle, continued marked improvement. The 4,000 meter race ended with Schofield improving by 2:07 with a time of 22:25 and a place-winning 13th spot.
The Jones County/Midland Rebels' second football game of the season was an admirably close one. On Friday, September 9, they lost to the Faith Longhorns 24-29. Five minutes into the first quarter, a Longhorn drive was completed for a touchdown when Garret Fordyce ran in the four remaining yards. The extra point attempt failed. With only a minute left in the same quarter, Ryan Palmer ran seven yards to score a Longhorn second touchdown.
In a strictly running offense and an aggressive defense, the Scotties rushed for 48 points and held the Lyman County Raiders to a zero score. The away game on Friday, September 9, ended 48-0 and gave the Scotties their second win in as many games. This is the first year as head coach for Keven Morehart. Last year he was on staff as a Lyman County Raider. The Philip team is extremely deep in quick and agile offensive runners, thus a passing game is not the main game plan. The first touchdown was run in by Bo Slovek, and Casey Cuny ran in the two point conversion.
Eileen June Brooks was born in Cottonwood, SD, in 1929. She was the daughter of Roger J. and Rena Peterson. She married Melvin D. Brooks of Philip, SD, December 27, 1944. Together they had three children, Coleen Calero, of Dana Point, CA, Roxanne Blum, Henderson, NV, and Brian Brooks, of Laguna Niguel, CA., and one granddaughter. Melvin preceded her in death in 1970. She was a resident of Palm Springs, CA, for over 30 years.
Darrel Malone, Jr., also known as "Paddy" to his family and friends, passed away recently in a Denver, CO, area nursing home, where he had been a resident for several years. Memorial services will be held Friday, Sept. 15, at Ft. Logan, CO, with burial in the Ft. Logan cemetery. Paddy's father, Darrel Malone, Sr., passed away a few years ago and his mother, "Red" Malone, is residing in a nursing home in Yuma, AZ. The Malones and Lytles are former Philip residents and owned and operated the Midway Cafe for many yeras. Paddy graduated from Philip High School.
Joseph James McNeese, 54, Belle Fourche, died Saturday, September 3, 2005, at his home in Belle fourche following a battle with cancer. his wife and children were at his side. Joe was born May 15, 1951, in Fargo, ND, to Thomas James McNeese and Angela (Cossette) McNeese. Joe grew up mostly in Fargo, graduating from Fargo Shanlee, and he attended NDSU. Working in the construction industry, he married Lori Hess in Lemmon, SD. His work with Tri-State Construction brought them to Belle Fourche. Two sons, Brandon and Quillen were born to this union.
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Tea will be served at 11:45 AM.
Cancer Biology and Genetics Program
Graham Walker, PhD
American Cancer Society Professor
Massachusetts Institute of Technology
Cambridge, MA
This program is for the research community.
Memorial Sloan Kettering Cancer Center
Zuckerman Research Center
417 East 68th Street
Room ZRC-105
New York, NY 10065


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This product has additional required/recommended options. To configure, add to your cart.
Wall Plate Required
Lutron DVLV-600P Diva 450 Watt 120 Volt Single Pole Electronic Low Voltage Preset Dimmer with Locator Light
Single pole 120 volt 450 watt Electronic low voltage preset dimmer with nightlight. The Diva paddle switch automatically returns to your favorite pre-selected light level.
A simple and elegant solution designed to match your existing designer style switches and accessories. Easily turn the lights on and off with the paddle switch.
Lutron DVLV-600P Features:
- Matches existing designer-style switches
- Features a large paddle switch and a small slider
- Single-pole model
- Matching Claro® gloss or Satin Colors® wall plates available
- Power failure memory
- Standard RFI suppression
- UL and CSA Listed
- Rated at 120 VAC, 60 Hz
Lutron DVLV-600P Specifications:
- Single-Pole
- Voltage: 120
- Wattage: 450
The story of Lutron began in a makeshift lab in a New York City home in 1959 where Joel Spira emerged with a radical new innovation in home lighting: the solid-state rotary dimmer. In almost 50 years of innovation, Lutron has invented hundreds of lighting control devices and systems, and expanded their product offering from 2 products to 15,000. Lutron dimmers not only set the perfect mood in a home, they also reduce energy consumption and extend lamp life. Taken as a whole, Lutron light controls have reduced electrical use by 9.2 billion kWh, reducing their customers' electric bills by $1 billion annually. The company has advanced the technology of lighting control while focusing on exceptional quality and design. Since the beginning, the company has maintained exceptional service, offering 24-hour technical support for its products, and a friendly customer service department.
Additional Lutron Links
This Lutron item can also be referenced by the following color/finish specific model #:
- Lutron DVLV-600P-AL
Almond - Lutron DVSCLV-600P-BI
Biscuit - Lutron DVLV-600P-BL
Black - Lutron DVSCLV-600P-BG
Bluestone - Lutron DVLV-600P-BR
Brown - Lutron DVSCLV-600P-DS
Desert Stone - Lutron DVSCLV-600P-ES
Eggshell - Lutron DVSCLV-600P-GS
Goldstone - Lutron DVLV-600P-GR
Gray - Lutron DVSCLV-600P-GB
Greenbriar - Lutron DVSCLV-600P-HT
Hot - Lutron DVLV-600P-IV
Ivory - Lutron DVLV-600P-LA
Light Almond - Lutron DVSCLV-600P-LS
Limestone - Lutron DVSCLV-600P-MR
Merlot - Lutron DVSCLV-600P-MN
Midnight - Lutron DVSCLV-600P-MS
Mocha Stone - Lutron DVSCLV-600P-PD
Palladium - Lutron DVSCLV-600P-PL
Plum - Lutron DVSCLV-600P-SG
Sea Glass - Lutron DVSCLV-600P-SI
Sienna - Lutron DVSCLV-600P-SW
Snow - Lutron DVSCLV-600P-ST
Stone - Lutron DVSCLV-600P-TP
Taupe - Lutron DVSCLV-600P-TC
Terracotta - Lutron DVSCLV-600P-TQ
Turquoise - Lutron DVLV-600P-WH
*Discontinued model or finish
More Than Just a Store - Your Reasons to Shop
Discover the best online deals for the DVLV-600P and everything else for your home from the leading online home improvement store.
We're committed to providing you with the best assortment of Lutron products with over 400 warehouses across the country.
Our knowledgeable product specialists work to bring you the best prices on every product and provide unmatched customer service. Your satisfaction is our highest priority!
Manufacturer Resources
Installation Resource Center
Whether you are installing the product yourself or hiring a professional, our installation Resource Center has everything you need to get your project done. Visit the Installation Resource Center to get started.


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Welcome to the official Roloff Family Website
Matt, Amy, Jeremy, Zachary, Molly and Jacob are best known for TLC's family-oriented reality show, Little People, Big World and LPBW: Wedding Farm.
We strive to keep this site warm and welcoming for our long-time fans and first-time visitors. Be sure to check out our entire site and come back often for frequent updates.


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Radio Control Airplane
This is an uncovered assembled Radio Control Airplane called a 60 size Carl Goldberg Tiger 2.
The Ryobi 1/4 pad sander with 220 and 600 grit sand paper was used for the final sanding of the balsa wood frame before covering. While the sanding could be done by hand, I found the palm size vibrating finish sander to do a quick and easy job of smoothing the framing befor the covering process. My policy is never use a hand tool when you can use a power tool! Thanks Ryobi for the great power tools.


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LAKE TAHOE - Lake Tahoe could become the new host of the Winter X Games.ESPN, which owns the X Games franchise, announced qualified host sites on Thursday, and Lake Tahoe was listed among the eligible destinations for 2015-17. Finalists will be determined by April 2013 and host cities will be announced by the end of 2013. The X Games bid is sponsored by the Lake Tahoe Sports Organizing Committee, a new entity created to bring marquee events to the area and generate overnight stays while promoting Lake Tahoe to a global audience. Committee partners include the Lake Tahoe Visitors Authority, North Lake Tahoe Chamber/Convention & Visitors Bureau/Resort Association, Heavenly Mountain Resort and Squaw Valley, among others. "Hosting an event of this caliber would provide significant economic impact for the destination," said Carol Chaplin, executive director of the Lake Tahoe Visitors Authority. "The demographics of the X Games align perfectly with attractions and appeal of Lake Tahoe and its combination of outdoor recreation, spectacular natural beauty and 24-hour lifestyle." According to ESPN, past X Games winter events have yielded substantial results for host cities. Aspen estimates that the X Games generates up to $10 million in identifiable benefits. The event, historically held in January or February, has attracted 20,000 to 25,000 spectators per day. The broadcast distribution alone for the X Games Aspen 2012 reached 232 million homes in 192 countries and territories. "The X Games is a unique opportunity to showcase winter sports in Lake Tahoe to a targeted domestic and international audience," said Andy Chapman, chief marketing officer of the North Lake Tahoe Chamber/Convention & Visitors Bureau/Resort Association. "It's a fantastic chance to tell the world about our phenomenal snow conditions, prestigious training grounds and the many and varied resorts that make up Lake Tahoe."The inaugural event was in 1995 and includes extreme sports (hence the name) ranging from Snowboarder-X (Boarder Cross), Slopestyle, Superpipe, Big Air/Best Trick, Snocross and Freestyle Snowmobiling. Competitions would be conducted at Squaw Valley and Heavenly."We are proud to be associated with Vail Resorts and Heavenly along with our regional partners to work to bring the X Games to Lake Tahoe," said President and CEO Andy Wirth of Squaw Valley and Alpine Meadows. "This is truly a partnership of high impact players from the region with a great deal of experience planning and staging world-class events. Over the last few decades, we've watched the X Games evolve. At its founding, the X Games revolutionized the landscape of winter sports and it continues to evolve and remain the single most impactful, admired and esteemed winter events, period. We sincerely hope to be a part of it the X Games' incredible evolution."
- Across the Universe: We shouldn't limit the ability to choose
- Nepal earthquake: Tahoe-led trekking group was on Mount Everest when everything 'happened so quickly'
- $500 million Lake Tahoe ski resort redevelopment won't break ground in '15
- Hundreds evacuated after MontBleu fire
- Tahoe native, FDNY firefighter heads to Nepal to aid earthquake survivors


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Sandra Dress
Out of Stock
A twist on the bodice centers a soft pleat on this figure-flattering empire waist dress in our sensually soft stretch knit. The moderate V-neck is bra-friendly and offers coverage that makes this dress a likely candidate for the office.
- Double-layered knit bodice for soft support.
- Shirring on the shoulders drapes fabric on the bodice.
- Approximate length: 37.5".
- 95% Polyester, 5% Spandex. Machine wash, cold; tumble dry low. Imported.
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Lake Hill Winery
Lake Hill Winery was built, not only to be a winery, but to be a gathering place and have the ability to hold a wide variety of functions. Besides the wine production, there is a tasting room bar, a small gift shop, and two banquet rooms. The first is the barrel room, which holds 100 people. This room is typically booked for smaller events such as reunions, rehearsal dinners, showers, company parties, birthday parties, etc. The other banquet room is the main hall. With two levels, capacity for over 400 people, a 160" TV/projector, and two bars, this room is capable of accommodating almost any event.
Diamond Dave's Mexican Restaurant
Casual Mexican-American dining for the whole family. Enjoy one of the 14 flavors of margaritas.
The Wright Place
The Wright Place offers home cooked meals, banquet facilities for up to 130 guests, breakfast served all day along with fresh pies daily. BBQ Ribs and fresh cut ribeye steaks on Fridays.
Wood Inn
The historic Wood Inn has been in operation under the same name since 1891. Now the main attraction besides the great food is the famous "wood chips." They are also known for great burgers, tenderloins and steaks. Located just off the NW corner of the square in Carthage.
Double Deuce
Whether you stop by after work to join in on the banter around the "u" shaped bar or come for breakfast, lunch or dinner you are sure to enjoy your time at the Double Deuce located in Carthage. Serving home cooked meals daily with affordable prices and a wide variety of menu options to please any appetite.
Roseville Lanes Diner
Family-owned restaurant and bowling alley with a banquet facility available.
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Deliver some holiday cheer to your family and friends with the help of our stylish holiday photo cards. Our unique and modern designs are sure to be a perfect fit with your Holiday Photo. Add a personal touch to it is easy as each design can be customized with own colors, photos and personal greetings. Our new holiday photo cards are sure to bring you and your family a little closer to your loved ones from afar.
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Unique and Customizable designs
This holiday photo cards are one of a kind for our friends and family. The photos we used work so well with the design and we can't wait to mail these out over the weekend, I know everyone will like them! I will enthusiastically recommend Love vs Design to anyone I know. I can't say enough good things about how easy they made this process!
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The design fit perfectly!
There were so many awesome designs it was hard to choose just one! But with the help of the Love vs Design customer service team, they answered questions fast, literally right away. We were able to find the perfect holiday card to fit our Modern family photo style with great quality. I can't wait to send these keepsakes to our family and friends!!!
This cozy Holiday card has all the elements you need to spread warmth and good cheer this holiday season, from snow flakes and deer, to owls, feathers and birds! The kraft background color gives it a whimsical and rustic look. A designer will create a personalized proof of your Winter Animals holiday card design within 3 business days for approval before printing. 3 free digital PDF proofs are included with each order.
Size: 5" x 7" | 127mm x 178mm
Design by: Love vs Design
Photo by: Studio Castillero
Photo requirements and tips
Customization options: You have the option to change the design colors and holiday card wording "Happy Holidays" into any seasonal greeting you like. Turn this card into a family christmas card, hanukkah card, newlywed holiday card, baby's first christmas card, new year cards, corporate holiday card, etc.
Details: animals, dear, birds, owl, kraft, feathers, snow flakes
Customization questions? Click here for |
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Posted on 09/01/2012 9:19:37 AM PDT by Whenifhow
Today, President Obama will sign an Executive Order directing key federal departments to expand suicide prevention strategies and take steps to meet the current and future demand for mental health and substance abuse treatment services for veterans, service members, and their families.
Ensuring that all veterans, Active, Guard, and Reserve service members and their families receive the support they deserve is a top priority for the Obama Administration. Since September 11, 2001, more than two million service members have deployed to Iraq or Afghanistan with unprecedented duration and frequency. Long deployments and intense combat conditions require optimal support for the emotional and mental health needs of our service members and their families. The Obama Administration has consistently expanded efforts to ensure our troops, veterans and their families receive the benefits they have earned and deserve, including providing timely mental health service. The Executive Order signed today builds on these efforts.
President Obamas Executive Order
The Executive Order signed by President Obama:
Strengthens suicide prevention efforts across the Force and in the veteran community:
The Executive Order directs the VA to increase the VA veteran crisis line capacity by 50% by the end of the year. Under the Executive Order, VA will ensure that any veteran identifying him or herself as being in crisis connects with a mental health professional or trained mental health worker within 24 hours or less. VA will work with the Department of Defense to develop and implement a national 12 month suicide prevention campaign focused on connecting veterans to mental health services.
Enhances access to mental health care by building partnerships between VA and community providers:
In service areas where VA has faced challenges in hiring and placing mental health service providers and continues to have unfilled vacancies or long wait times, the Executive Order Directs the Department of Veterans Affairs to work with the Department of Health and Human Services (HHS) to establish at least 15 pilot sites. In pilot sites, VA will contract with community health centers, community mental health clinics, community substance abuse treatment facilities and other HHS grantees and community resources to help reduce VA mental health waiting lists. Under the Executive Order, HHS and VA will develop a plan for a rural mental health recruitment initiative to promote opportunities for VA and rural communities to share mental health providers when demand is insufficient for either to support a full-time provider.
Increases the number of VA mental health providers serving our veterans:
Under the Executive Order, VA will hire 800 peer-to-peer support counselors to empower veterans to support other veterans and help ensure that their mental health care and overall service needs are met. VA has launched an effort to hire 1,600 new mental health professionals to serve veterans. The Executive Order directs VA to use its pay-setting authorities, loan repayment and scholarships, partnerships with health care workforce training programs, and collaborative arrangements with community-based providers to recruit, hire, and place 1,600 mental health professionals by June, 2013. Since, 2009, the VA has expanded its mental health programs, hiring more than 3,500 mental health professionals since 2009.
Promotes mental health research and development of more effective treatment methodologies:
The Executive Order directs the Department of Defense, the Department of Veterans Affairs, the Department of Health and Human Services and the Department of Education to develop a National Research Action Plan that will include strategies to improve early diagnosis and treatment effectiveness for TBI and PTSD. The Executive Order further directs the Department of Defense and Department of Health and Human Services to conduct a comprehensive mental health study with an emphasis on PTSD, TBI, and related injuries to develop better prevention, diagnosis, and treatment options.
Launch a government-wide collaborative effort to address these issues through a Military and Veterans Mental Health Interagency Task Force:
The Executive Order establishes an Interagency Task Force, including the Department of Defense, Department of Veterans Affairs, Department of Health and Human Services, Department of Education, the Domestic Policy Council, National Security Staff, the Office of Management and Budget, the Office of Science and Technology Policy, and the Office of National Drug Control Policy, which will make recommendations to the President on additional strategies to improve mental health and substance abuse treatment services for veterans, service members, and their families.
Supporting our Military, Veterans, and their Families The President has taken key steps to protect and strengthen the health of our military, veterans and their families here at home. Many of these initiatives are supported by agencies across the federal government and collaborative partnerships with states and communities.
Health Care
For the first time ever, 135 medical schools have committed to exchanging leading research on PTSD and TBI and will also train future physicians to better understand veteran health needs. More than 150 state and national nursing organizations and over 650 nursing schools have committed to ensure our nations 3 million nurses are prepared to meet the unique health needs of veterans and their families by educating the current and future nurses of America to have a better understanding of PTSD and TBI. President Obama signed the caregivers and Veterans Omnibus Health Services Act of 2010, into law which helps our most seriously injured post-9/11 veterans and their family caregivers with a monthly stipend; access to health insurance; mental health services and counseling; and comprehensive VA caregiver training and respite care. The Department of Labor has proposed new regulations for the Family and Medical Leave Act (FMLA) to support military families and caregivers. This rule would implement statutory changes to the FMLA, expanding leave to family members caring for veterans who have suffered a serious injury or illness. In July 2010, the VA published a historic change to its rules, streamlining the process and paperwork needed by combat veterans to pursue a claim for disability pay for post-traumatic stress disorder (PTSD). The VA expanded its workforce by over 2,600 people to handle applications for disability pay. The VA is also using technology and new approaches to help veterans get their benefits by accepting online applications for initial disability benefits, initiating an innovation competition, launching pilot initiatives, and investing over $128 million in a paperless Veterans Benefits Management System. The administration is utilizing partnerships to reduce the stigma associated with seeking treatment for behavioral health issues. Make the Connection, a campaign launched by the Department of Veterans Affairs, is creating ways for veterans and their family members to connect with the experiences of other veterans and access the information and resources to help these families confront the challenges of transitioning from service to daily civilian life.
Licensing and Credentials
Nearly 35 percent of military spouses in the labor force require licenses or certification for their profession. Many military spouses hold occupational licenses and routinely move across state lines, causing licensing requirements to disproportionately affect the military spouse population. The First Lady and Dr. Biden encouraged all 50 governors to pass legislation by 2014 to reduce the financial and administrative strains that 100,000 military spouses incur from trying to get their state licenses or certification credentials to transfer from state to state as they move. Mrs. Obama and Dr. Biden encouraged governors to take Action in February 2012 when only 11 states had legislation on the books. 26 states now have measures in place to support military spouses and the initiative is on-track to meet the 2014 goal.
The Department of Defense has awarded $180 million in grants to support military-connected public school districts. These grants support improved academic programs for military children. More than 400,000 students from military families across all grade levels are impacted by these grant projects. The Department of Defense has awarded approximately $25 million to military-connected Local Education Agencies (LEAs) this summer to focus on increasing student achievement and easing transitions through research-based academic and support programs. The Department of Defense, in collaboration with the Council of State Governments (CSG) National Center for Interstate Compacts developed the Interstate Compact on Educational Opportunity for Military Children (the Compact) to address the educational transition issues of children of military families. The Compact covers transition issues including class placement, records transfer, immunization requirements, course placement, graduation requirements, exit testing, and extra-curricular opportunities. States adopt the Compact through legislation, and as a result, join the Military Interstate Childrens Compact Commission (MIC3). To date, 39 states have approved the Compact and these states are home to 89 percent of school age children whose active duty parents are assigned to military installations in the United States. We will continue to work with leaders to encourage the 11 remaining states approve the Compact and become members of MIC3. VA eased the Post-9/11 GI Bill application process within the eBenefits portal, including transferability to spouses or children for service members with over six years of service. Servicemembers can now apply on-line to transfer the benefits of their Post-9/11 GI Bill to eligible beneficiaries.
On top of the historic settlements completed by the Federal government and 49 state Attorneys General, major mortgage servicers will be providing relief to thousands of service member and veteran households. A review will be conducted of every service member household foreclosed upon since 2006. Those wrongly foreclosed upon will be compensated equal to a minimum of lost equity, plus interest and a refund for money lost because they were wrongfully denied the opportunity to reduce their mortgage payments. Additionally, these organizations will pay $10 million into a VA fund that guarantees loans on favorable terms for veterans. The Administration is working to end veteran homelessness through leveraging broad support at Federal, State, and local levels in both the public and private sectors. Working with over 4,000 community agencies, the VA and HUD have successfully placed more than 37,000 veterans in permanent housing with dedicated case managers and access to high-quality VA health care since 2009. To ensure we reach out to our homeless veterans, the VA created a National Registry for Homeless Veterans and established a National Homeless Hotline. Veteran homelessness was reduced by nearly 12 percent between January 2010 and January 2011. In 2011, VA helped save 72,391 Veteran and military borrowers with VA-guaranteed loans from foreclosure, a 10% increase from the prior year. VA has helped nearly 59,000 borrowers avoid foreclosure so far in 2012. The home loan guaranty program helps Veterans and their families purchase homes, often with no down payment required. The program expects to guaranty the 20 millionth loan in early November 2012. Using their Interest Rate Reduction Refinancing Loan, also known as the Streamline Refinance, VA refinances existing VA loans into new loans with lower interest rates, or adjustable rate mortgages (ARMs) into fixed rate mortgages. In 2011, this program saved an average of $202 per month in individual payment reductions and 1.42% in interest rates. This equates to saving military and veterans $24 million a month and $293 million per year.
Financial Readiness
The Department of the Treasurys Office of Financial Education and Financial Access has helped military families identify predatory lending practices. The Consumer Financial Protection Bureau (CFPB) established an office of service member affairs to ensure that the CFPB addresses the financial challenges that confront military families and strengthens protections against abusive financial practices.
Source: White House Press Release
Full title: President Obama Signs Executive Order to Improve Access to Mental Health Services for Veterans, Service Members, and Military Families
Well that's that! He just wrapped up the military vote!!~sarc
Oh, this will help vets like the Facebook poster. /s
What it really does is to allow all the Feds to track vets online, to track them at home, at work and to pick them up for psych evals at any given time, especially if they seem to be gaining traction in sending messages of dissent against the Obama regime.
Yeah, Veterans' mental health is SO IMPORTANT to Obama, that he concocts this EO 2 months out from election day.
What a phony creep.
Touche'. It means that all of them who have weapons at home will be disarmed by authorities.
The VA already has lots of mental support available right now. When calling to the local VA clinic, their options menu asks "if you are having thoughts of harming yourself right now, dial _ ".They realize that suicide is a HUGE issue.
Typical Obama pandering crap, hoping to "sequester" more dissidents under the guise of "helping" them.
Communist SOB. Straight out of the Mao handbook.
As a Vet, whatever the motivation, improvement in VA abilities to provide much needed services is not a bad thing.
To clarify. If a vet goes to a VA facility, he'll be asked at his initial intake if he has weapons at home and he'll be asked if he has now, or ever had, suicidal thoughts. Those who answer "yes" will be flagged for further evaluation. I don't know what the thresh-hold is requiring VA personnel to signal authorities to go and remove the vet's personal weapons from his possession.
He's rounding up vets right now under phony anonymous fear statements, now he's just allocating money to expand that ability.
Beware of commies bearing gifts.
"Soviet psychiatric hospitals known as "psikhushkas" were used by the authorities as prisons in order to isolate hundreds or thousands of political prisoners from the rest of society, discredit their ideas, and break them physically and mentally."
Yes, this is similar to what the Soviets did to political dissidents.
Not "unexpected" like everything else these days. Barry and the 'RATS think all military people and their families are crazy and need psychiatric help.
POTUS Obama is not going to 'give' anything to or for the military. This is just legal traction to be to arrest and institutionalize a disident without trial and with limited legal recourse.
Obama would be only too happy to see every military veteran commit suicide. He would even be will to assist them. His fear is that they are potentially a rela threat to his obtaining total power like Chauvez, et al.
Getting rid of this entire sub-standard organization,the VA that is,which seems to be completely corrupt not to mention how invasive of Veterans privacy they've become would be a great step for this country.
Otherwise replace it with something else and launch an investigation in the people who are working there.They are doing an enormous disservice towards the taxpayer and the people who've served this country.
Otherwise you will never replace a decent living environment,which this country doesn't seem able to provide in the post-Bush years,with a shrink.No matter who or what he works for.
""a top priority for the Obama Administration.""
I don't know how he keeps track of all his TOP PRIORITIES!!
I think getting to his desk and picking up a pen is top priority to him.
We all know what his REAL top priority is!!!
He's the one who needs access to mental health services.
There is a hook in that. When one applies for mental health services, one loses their Second Amendment rights.
Now they have federal authorization to create more cases like Mr. Raub, hauled off to a mental institution for posting anti-government comments on Facebook. No legal recourse until his mother was put in touch with the Rutherford Institute.
This is all BS. I work for a veterans group.
This is all going to support the bloated govt... in the name of veterans. The money goes to agencies and the contractors NOT the veterans.
Obama cut GI bill and combat compensation in 2009 and 2010...this is money that goes directly to vets and not a govt agency.
Please dont let this go on unchallenged.
Apparently they are satisfied with the test results.
As a veteran, NO Way! I recommend all veterans and active duty do NOT have anything to do with this. The left is using it as an excuse to take away our rights to own firearms. Piss off government!
Thank you for the inside view. I've already seen this in action.
The words "Executive Order" mean loss of freedom in our republic.
The current president apparently thinks he is a king, or chief in some thirdworld rathole.
The VA has been pounding the ground sending out questionaires asking whether Vets need more mental health services in the area. They really don't but I think it has been a way to round up funding and grants.
Last year a woman came to our town and proposed a study to discover the extent of the drug use in the community.
She wanted a 30K grant.
Instead the town needed two cops to police the area of drug dealing in the town, and push the creeps right out of town.
That is what the town to the south did.
Not what our town did.
We gotta keep it moving and not let them get away with it.
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text: Beat the Heat! That's a phrase with a double meaning now in Dallas. What a ride these playoffs have been. From such low expectations this season after the debacle of 2006 and playoff failure after failure from the loss to 8th seed Golden State the following season to the San Antonio loss in 2010.


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New book chronicles woman's life struggles and victories
UPDATED 4:25 PM EDT Mar 11, 2013'Cry Purple' is a new book that chronicles a woman's struggles as a prostitute and addict who reconnects with her family in Kansas City, all without her sight.
The views expressed are not those of this site, this station or its affiliated companies. By posting your comments you agree to accept our terms of use.blog comments powered by Disqus


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Algebra teacher Brielle Dana, 27, will go for the gold in Israel this summer at the 19th World Maccabiah Games, an international competition held every four years that brings together top Jewish athletes from across the globe.
Dana of North Bethesda is one of 7,000 people from 50 countries who will compete in the games this summer. She was chosen to represent Team USA in the triathlon for women ages 25 to 29. The event includes a nearly milelong swim, 25-mile bike ride, and 6.2-mile run.
"I have dedicated a lot of my time and energy to the sport," she said. "I would love nothing more than to do a triathlon in Israel, and to be among other Jewish people who are doing the same thing that I am."
To qualify for the Maccabiah Games, Dana submitted a detailed spreadsheet of her times for all her races to the International Maccabi Committee, which organizes the competition.
She did six triathlons this past season, finishing first out of 22 people in her age group in her last triathlon competition, the Giant Acorn International at Lake Anna State Park in Virginia. She scored sixth overall with a finish time of 2:25:28.
Dana is in the best shape of her life, but it is her positivity that has most impressed her boyfriend of nearly three years, Marc Lizmi of North Bethesda. When the two first met at Tommy Joe's in Bethesda, Dana had not yet discovered her love of triathlons. He said the sport has given her an energy she brings with her to work and all of her relationships.
"When she's dominating the competition she does it with such grace and such poise, you forget that she was competing in the first place — you think, she's just doing something she loves to do," Lizmi said. "The training in itself is so intense, I think most people would be overwhelmed. It's torture, right?"
When she is not training young minds at Winston Churchill High School, she can be found at the gym, following a strict regimen of diet and exercise she admits many would find grueling.
Her day begins at 5 a.m., when she gets up to swim at the Kennedy Shriver Aquatic Center in Rockville, or lift weights at Washington Sports Club in Bethesda. She also lifts weights after work four days per week, goes to spin class three days per week, and runs at least three days per week.
"It's addicting," she said. "I just have this constant drive to push myself physically, mentally. Every race I have been able to drop my times."
Her meals are restricted mostly to fruit, chicken, brown rice and vegetables, a diet set by her coach to keep her body gym-ready. But she said she still takes time to treat herself.
"Every weekend my boyfriend and I, after my training session, I get a big ol' bagel," she said.
A Rockville native, Dana graduated in 2003 from Wootten High School, where she competed in field hockey and track. She also was on the swim team at Rockshire Pool in Rockville. In college at the University of Maryland, College Park, she started long-distance running, and took up long-distance cycling after college.
After finishing a couple of 100-mile bike rides known as century rides, Dana was ready for a new challenge.
"I thought, "What if I put them all together, and I started training for a triathlon," she said.
Her first triathlon was in September 2011, and she has loved it ever since.
Her current challenge is to raise $6,000 by February so she can afford to go to Israel in July for the games, where she must pay for travel expenses, uniforms, room and board. So far she has raised nearly $1,700 thanks to donations from friends, family, and a fundraiser at her gym lead by her spin class instructor.
"Whatever it takes I will get there, even if I have to go into debt to do it," she said. "I want to get there no matter what. This is a once-in-a-lifetime opportunity that I wouldn't want to pass up."
To donate, go to http://bit.ly/SfSniL.


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Modeling Thermal Stresses of Copper Interconnects in 3D IC Structures
Bentz, D.N., Zhang, J., Bloomfield, M., Lu, J-Q., Gutmann, R.J., Cale, T.S.Rensselaer Polytechnic Institute
One of the key issues in developing higher density microelectronics devices is the impact of the stresses induced by thermal expansion mismatches of the materials used.
We have examined, using FEMLAB, the stresses due to interwafer copper interconnects embedded in multilayer structures created by bonding two wafers using an organic low-k dielectric glue, benzocylcobutene (BCB). This work indicates that there are reasons to be concerned about stress induced failures, in both the Cu vias and in thinned Si wafers, due to differences in coefficient of thermal expansion (CTE) values for relevant materials.
As experimental stress data on 3D ICs are not readily available, we validate our simulation approach using experimental data from two types of planar systems.
After validating our simulation approach using experimental data from planar systems, we applied it to the 3D IC system [1]. The system of particular interest is that of Cu inter-wafer vias through a seven -layer 3D IC stack terminated with a Cu pad.
Depending upon the pitch and size of the Cu vias, for a given BCB thickness Cu failure is a concern. Conversely, when Cu pitches are small and/or Cu vias are large enough, failure of the thinned Si wafer is possible. We suggest using a collar, made from a low modulus dielectric to minimize this risk.
This topic is continued in id: 1347.


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Course Catalog
PSYC3650 - Psychology Of Gender
Summer 2014 This course examines the topic of gender from a psychological perspective - the behaviors and attitudes that relate to (but are not entirely congruent with) biological sex. This course explores gender as a psychological construct and a situational variable that influences our experiences in a number of contexts. We will examine the various methods used to study gender and evaluate research associated with specific gender-related issues. This course will emphasize variability within sex and gender categories, including issues associated with culture, race, gender identity and sexual orientation. Prerequisite: PSYC1010 3 Credits |
Schedule Book for All Active and Available Future Terms, Course starting with PSYC3650
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LEGAL GUEST BLOG -- CHARLES SCHAEFER How binding are letters of intent?By CHARLES SCHAEFER, ESQ. 4:30 am, October 12, 2012
In last week's blog posting, we discussed how to prepare your business and dress up your bottom line in anticipation of a sale or merger. This week, we take a step farther down the bumpy road of mergers, acquisitions and divestitures to evaluate the pros and cons of one of the most important documents in the initial phase of "courtship" – the letter of intent.
PRINTED FROM: http://www.crainscleveland.com/article/20121012/BLOGS05/310189997/-1/NEWS04&template=printart |