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Series: Saturday Science Links (21 entries)
The biggest science stories of the week are reviewed.
The Resource Finder has powerful filtering features — check out the tutorial to learn more.
The biggest science stories of the week are reviewed.
From geology to biology to research into the origin of life itself, the power of water shows the faithfulness and creativity of our Creator.
In the early 1700s, Isaac Newton and Gottfried Wilhelm Leibniz famously disagreed about whether God periodically "corrected" the orbits of the planets. Why their disagreement matters for today's debates about God and evolution.
A common challenge to evolutionary theory is that while life does indeed change over time (what is known as microevolution), no one has ever seen one species evolve into another species (macroevolution).
Evangelicals are skeptical of space exploration—but can pastors change this from the pulpit?
From the archives: A common argument leveled against the theory of evolution is that scientists have not been able to produce transitional fossils that show the change of one species into another. In this podcast, we address a common misconception about what transitional fossils actually are.
The former chair of the Harvard University physics department muses on how faith gives meaning to his scientific work.
The story of the Genesis Rock reminds us of how biblical and scientific accounts, despite their different purviews and purposes, remain inexorably linked in our understanding of origins.
Are black holes in trouble? Could life have come from space? What do we know about the religious lives of Indian scientists? Find the answers to these questions and more.
This week's news features volcanoes, skeletons, and stars, as well as a thought-provoking new book from InterVarsity press.
From science and religion blogs: quantum uncertainty and God, the remarkable fact that we have come to understand our place in the created order, and the role of theology in making wise choices about the use of technology.
Written by BioLogos Fellow of Biology Dennis Venema, this series of posts is intended as a basic introduction to the science of evolution for non-specialists.
News about the Ebola outbreak and initiatives in genomic medicine lead our news roundup this week, with a collection of research and learning opportunities in biology and at the science/faith border to follow.
We don't have all the answers and never will. And when God says that he is revealed in his creation, I think that means we need to take care of what we have and understand where we came from. The more I understand how things work, the bigger God gets. When he was just a magician pulling things out of a hat, that doesn't even compare to how I see him now!
God could have created a fertile planet miraculously in an instant, but when we look carefully at the physical evidence, it appears that the earth has a longer history as part of the universe.
God could have created a fertile planet miraculously in an instant, but when we look carefully at the physical evidence, it appears that the earth has a longer history as part of the universe.
In this series, James Kidder provides an intriguing study on transitional fossils and the evolutionary history of modern humans. He begins by discussing the fossil record, explaining how new forms are classified. He then explains the physically distinguishing trait of humankind—bipedalism. From the discovery of Ardipithecus, the earliest known hominin, to the australopithecines, the most prolific hominin, Kidder focuses on the discovery, the anatomy, and the interpretation of these ancestral remains.
Geologist Keith Miller examines the "Cambrian Explosion", a period of rapid evolutionary diversification approximately 575 million years ago, and whether it poses a challenge to evolutionary theory. From the June 2014 issue of Perspectives on Science and Christian Faith from the American Scientific Affiliation.
When read in this way, the Genesis stories may be seen as one of the greatest-ever revolutions in human understanding. Genesis used the literary forms of the day to introduce its readers to a rational God who cares for people – and whose faithfulness made science possible. Genesis is expressly about theology, not science.


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Jewelry for Children Bracelets
This tender, loving collection for children offers fine gold chains that are precious heartfelt tokens to mark life's milestones.
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subset: commoncrawl


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I have 9.1-PRERELASE and I've had it since before Thursday:
hiryu@hostname:~$ uname -a
FreeBSD hostname 9.1-PRERELEASE FreeBSD 9.1-PRERELEASE #0: Sat Aug 18 17:16:25 PDT 2012 root@hostname:/usr/obj/usr/src/sys/HOSTNAME amd64
hiryu@hostname:~$ uptime
9:57AM up 7 days, 15:54, 3 users, load averages: 0.02, 0.03, 0.00
hiryu@hostname:~$ date
Sun Aug 26 09:59:54 PDT 2012
I also verified I have the latest source for 9.1 today. Why is it 9.1-PRERELEASE instead of RC1? I don't know... But maybe the cvsup mirror I'm using is lagging behind? But that would be a few days behind. Maybe I'll ask on IRC.
Note: I run FreeBSD on my home server (as you see here, and since it's not so mission critical, I can upgrade it to a PRELEASE without real cause for concern) and my server at the colo (which is still 8.3, I'm waiting for 9.1 before going to the 9.x series there). Otherwise I run Kubuntu as the desktop OS on my desktop, my laptop, and the family computer.


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The annual San Francisco Submarine Regatta will be held at the San Francisco Casting Club ponds on October 14th & 15th 2006. We will again have exclusive use of the far western pond. More information and a map will be posted in a few weeks.
Edited By anderboat on 1142866327


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Beyonce unflinchingly parses through fact and fiction about her high-profile pregnancy in "Life Is but a Dream," her new HBO documentary that debuts later this month. As Us Weekly revealed on Thursday, the 31-year-old opens up candidly in the film about the miscarriage she and husband Jay-Z endured before the successful January 2012 birth of daughter Blue Ivy.
The singer also puts to rest, once and for all, persistent speculation that she used a surrogate to carry Blue Ivy. Conspiracy theorists harshly claimed that Beyonce even sported a false baby bump in the nine months leading up to her baby's birth.
PHOTOS: Bey's pregnancy with Blue Ivy
"To think that I would be that vain," the Grammy winner retorts in the film. "I respect mothers and women so much. To be able to experience bringing a child into this world, if you're lucky and fortunate enough to experience that, I would never ever take that for granted."
She says of carrying a child: "It's the most powerful thing you can do in your life -- and especially after losing a child, the pain and trauma from that just makes it mean that much more. ... A child, you don't play around with that."
Calling her miscarriage the "saddest thing I've ever been through," she says becoming a mother to Blue became a truly life-defining moment. "My baby was born out of a conflict in my life and that struggle had to be settled. I wouldn't have gone through the pain if I hadn't gone down the path," she surmises. "There's something so relieving about life taking over you like that. You're playing a part in a much bigger show, and that's what life is, it's the greatest show on Earth. ... I felt like God was giving me a chance to assist in a miracle."
Beyonce plays the Super Bowl halftime show in New Orleans this Sunday. Singing the National Anthem live Thursday at a press conference for the event, she also acknowledged that she used a prerecorded track while singing the same song. "I did not have time to rehearse with the orchestra. It was a live television show. ... Due to the weather, due to the delay, due to no proper sound check... I did not feel comfortable singing live."
Still, this Sunday, she "absolutely" will be singing live, she assured reporters.
Check out the famous outfits Destiny's Child sported
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Margarita Maven
See how Sarah Michelle Gellar, Cindy Crawford and more stars celebrated Cinco de Mayo on today's Celebs Gone Social.
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'Kiss Me Quick'
Nathan Sykes addresses the One Direction feud and dishes on Ariana Grande and his new single "Kiss Me Quick."
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Princess Diary
In case you missed it, here's everything you need to know about Princess Charlotte in two minutes.
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Diva Double Date
Beyonce and Alicia Keys double date with their famous hubbies on today's Celebs Gone Social.
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Blondes Rule?
Do blondes or brunettes have more fun? Reese Witherspoon and Sofia Vergara dish at the Los Angeles premiere of "Hot Pursuit."
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Fashion Offenders
Fergie, Sarah Jessica Parker, and Heidi Klum all get an F on this week's Fashion Offenders.
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The Evolved User Experience
In today's fast-moving business market, a new product can fail as quickly as it was launched if, by the time it reaches the market, it's either less useful and engaging, or isn't innovative enough. At the core of the problem is the inability for companies to break away from more traditional approaches to product design and development process. This often means they design complex features that are dependent on a single big launch, but consequentially companies can't predict and design for future changes in user behavior trends.
Today, social technologies have made it less challenging for UX teams to involve users in the full project lifecycle, from initial phase of co-ideation, to ongoing iterations and testing during the design and development phases. In an environment where there is an ongoing relationship with the user that encourages flow of fresh ideas and feelings, changes in user behavior become more predictable compared to an environment where there's a very limited day-to-day relationship with the user.
Involving users in the product development cycle is of course not a new concept; it has been practiced by user-centered design (UCD) teams for a long time. The difference today is that social technologies enable ongoing user interaction with the product in real time, allowing product designers to vet ideas with users, and learn from users' concerns and firsthand experiences.
Social technologies offer opportunities for UX teams to lead the way in creating revolutionary concepts for product design and development as well as new and innovative ways of involving users in the product lifecycle.
The Next Frontier for UX Teams?
Today's users are empowered by technology as never before; technology has significantly changed the way users consume media, do business, and share information. Users are not anymore passive consumers of brands and user experiences—they're beginning to take ownership of them.
Technology has also changed the way product developers perceive the value of user insights. Product development teams are now getting a better understanding of the need to incorporate user feedbacks in the development cycles. They're getting increasingly involved either as active participants in or as observers of user research, participatory design, or even iterative user testing sessions conducted over crowd-sourced platforms. They're observing more closely how users express their feelings, desires, and needs about the product in a compelling way.
In a very real sense, access to real-time user information by product teams is helping create empathy towards users as never before and this in turn is encouraging teams to fully engage in their work with the belief that it will enhance and enrich users' experiences.
All this is very helpful if UX teams lead the process of identifying user audiences, collecting data, and extracting insights from the data. The enthusiasm of product teams for social technology platforms and the associated research methods and tools should not lead them to believe they don't need the help of UX team. Rather, it should pave ways for a more efficient collaboration with UX teams who not only see the value of actionable user insights but who also are experts in capturing and communicating the most relevant qualities of target audiences related to products and business strategies.
In the past, UX teams have had difficulty getting product teams to allow them to incorporate insights into the development process. But today, social technologies can allow UX teams to quickly and iteratively inject fresh ideas throughout the development cycle, enabling them to move faster to match the more agile product development cycles.
UX teams can have real impact on overall product strategy, but they must quickly adapt the new challenges and opportunities inherent in changing user behavior trends and evolving technologies in order to help provide a complete, consistent, and shared understanding of the user. They need to embrace the new technologies through which users are interacting with their brand, but still keep their focus on understanding user behaviors, and not necessarily the technology itself.
Users behave differently on social media platforms; they use these platforms in a radically different way than more traditional forums. They see social platforms as more integral parts of their lives. If UX teams learn to adopt these technologies in their work they will allow them to get insights about users in ways never would have been possible using traditional methods.
In most cases, UX teams will need to collaborate with other departments that already have access to, and an established process for disseminating, user insights from social media platforms.
Generating Insights From Social Media Platforms
Depending on where in the project cycle they are, UX teams have the appropriate platforms and technologies at their disposal. Online community sites can be used to start ambitious user research projects in which ethnographic methodologies are applied to learn about users. Similarly, social platforms can be used to identify the most loyal audience groups, for co-ideation and iterative design process. Ideally, to take full advantage of the opportunities that social technologies offer, the involvement of users in the product design and development cycles should be ongoing.
Despite the near-limitless opportunities social technologies can offer and the breakneck pace at which their capabilities are evolving, there are some challenges that can limit teams from using them effectively. Determining whether social technologies are being used by the target users or are even accessible to them is the first step in using social technologies as part of a product's development. Similarly, the limits and maturity of an organization's capability of understanding the value of insights found through social technologies is also an issue.
It can be an uphill battle for UX teams to inject these insights into the product development cycle when they're need to move at the pace that's required by the product team or when the product team is not bought in into the value of the insights for the user experience. To face down these challenges, UX teams need to evangelize the value of user insights to enhance the user experience and the business' success.
UX teams need to think beyond traditional user research methods and adopt new social technologies to take advantage of the opportunities that are provided by the technology in terms of understanding user behavior and leveraging them for user participation in the project cycles. In addition, these technologies allow researchers to be more agile and to iterate design ideas based on real user feedback.
In todays digital market the cost of not knowing your users better and not involving them in the product design and development process can be very high. Users are generous in providing positive feedback, but they also are ready to criticize the brand and product if they deem it appropriate.
The opinions expressed here are the author's own and not those of the American Cancer Society.
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Hello Alexander,
I enjoyed your article and agree with you on most points. The challenge we've run into is using social media to communicate and listen to your target audience without disclosing sensitive IP information that your competitors could review. Any thoughts?
Sounds logical to engage users in product development lifecycle, but there's a seismic difference between extracting useful insights from user behaviour and involving users in design. As Henry Ford said "If I had asked people what they wanted, they would have said faster horses."
You're exactly right Alexander. The profession needs to expand its methods into the social space.
We've recently completed a project here in the UK where we used Facebook as a platform for research to drive out user requirements for a mobile app for a big insurance brand.
Facebook have recently published a case study on their site:
For me this is just the tip of the iceberg in terms of using social media as a platform for customer collaboration and insight gathering.
Thanks for the article Alexander!
(Sorry for the appearance of shameless self-promotion.)
I wrote a paper a few years ago applying Jean Piaget's constructivist learning theory to usability. It maps directly to this topic:
"Few would argue that the technologies of the 1980s versus now could not be more different. Similarly, users do not interact with computers the same way they did in the 1980s. They've learned and adapted. More specifically, they've applied what they learned about interacting with 1980's systems and built on that knowledge to interact with 1981's systems and so on."
"This type of experiential learning evokes the constructivist theory, which states in part that "there is no single valid methodology." Not surprisingly, an understanding of usability is closely tied to the manner in which people learn, and if one subscribes to constructivism, there is no single valid methodology for learning. Jean Piaget is widely credited with the formalization of constructivism as a learning theory. He theorized that through the processes of accommodation and assimilation, individuals construct new knowledge from their experiences and build on them."
"Piaget's constructivist learning theory maps well
with the concept of usability. In a nutshell, Piaget believed that when individuals learn, they augment an existing framework of knowledge by adding newly learned experiences without altering the existing framework. In the context of usability, users adapt and build their knowledge of interacting with digital systems. Look at Apple's iPhone, for example. It represented a revolution in the way people interact with devices. In actuality, the iPhone's designers made a radical departure from the way people act with hardware, but most of the iPhone's software features are built on usability principles found in other applications already in wide use. The iPhone is a revolutionary design that merges an existing framework of usability with a consumer's ability to learn new interactions without altering the existing framework."
Nice points Alexander, Appreciate the read. I completely agree the user is smarter than ever and getting smarter everyday. More research need be done during the process, but the hardest part is convincing stakeholders with tight budgets the benefit in ROI and efficiency in the long run.
Hopefully soon I will be able to spend more time researching and less time convincing!


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Republicans: Seven elected officials to switch to GOP
by Associated Press Published: May 30,2012
Tags: elected officials, elections, parties, party affiliation, political parties, politicans, Politics, public officials, voters, votes, Voting
AROUND MISSISSIPPI — The Mississippi Republican Party says at least seven local elected officials are switching to the GOP. State party spokesman Brett Kittredge says in response to a question from The Associated Press that the officials are from Leake, Jones and Newton counties. Party chairman Joe Nosef will announce the names of the new […] [...]


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Yum! Great online tool for creating custom-designed M&M's. Anyone up for some red UX Magazine ones?
Alex is CEO of Sideshow , an award winning creative agency. You can read his blog here.
Nice articles, but I am not creplica handbags replica bagslear about the point you mentioned about how to distinguish fake and real .


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Thursday, February 21, 2013
Prison Education Project Informational Meeting
Are you interested in tutoring incarcerated youth?
You have the opportunity to volunteer with the Prison Education Project (PEP), which is expanding educational opportunities for inmates in the California institutions for men, women, and youth.
Oxy students would volunteer at Barry J. Nordoff Juvenile Hall in Sylmar, CA once per week (90-minute sessions) for seven weeks.
Want to learn more? Come to an informational meeting with Renford Reese, Cal Poly Pomona Professor and the founder/director of PEP.
More information is available at http://www.
Thursday, February 21, 2013
Food Safety Class
Each club event where food is served must have a member in attendance who has completed Campus Dining's food safety class.
Students who took the class within the last three academic years may continue to serve in this capacity and are not required to take it again.
The class will provide guidelines for purchasing, preparing and serving food safely for club events, based on USDA recommendations for volunteers, and the California Food Code. Oxy's Catering Policy will also be explained. This is NOT a food safety certification class (such as ServSafe®).
RSVP: To register for the class, please send an e-mail to firstname.lastname@example.com, with the subject line: Food Safety Class. You will receive an e-mail confirmation back by Monday 2/18 if you are enrolled. RSVP is required.
Friday, February 22, 2013
125th Anniversary Speaker: Doug McAdam '73
"The Civic Impact of Youth Volunteerism: The Curious Contrast Between Freedom Summer and Teach for America."
Dr. McAdam is a Professor of Sociology and the Director of Urban Studies at Stanford University. The author or editor of 19 books, McAdam was awarded the 2010 Lifetime Achievement Award by the Center for the Study of Social Movements. In this licture at his alma mater, McAdam will contras his research on two student-led social justice campaigns: Freedom Summer, an effort in 1964 to reguster African American voters in Mississippi, and Teach for America, an ongoinjg program aimed at enhancing education in under-resourced urban and rural public schools.
Friday, February 22, 2013
OSL Taste Off
The OSL's monthly taste off event is starting off its new year with a showcase on hot wings from the local area!
The OSL's monthly taste off event is starting off its new year with a showcase on hot wings from the local area! Come try local spins on hot wings, and vote for the best one to be served at OSL's pub night! This is a great way to get some free, good food during the week that also shows off local neighborhood food establishments.
Friday, February 22, 2013
Clothing and Baked Goods Sale
The best part? Everything is just one dollar!
The second best part? All proceeds go to support your B.A. friends on Oxy's Women's Rugby team!
This Friday is going to be an epic day; donated clothing from your swaged -out classmates is going to be sold this Friday, along with yummy baked goods.
Also, if you're closet is still looking a little full you can still donate clothing. There are still collection boxes located in the common room of the residence halls. All you have to do is put unwanted clothing in it; we'll do the rest!
Friday, February 22, 2013
Aflac Jobs Info Session
Learn about exciting opportunities with the Aflac Corporation
Members of Aflac will be on campus to speak directly with students about job opportunities in the company. This is a great opportunity to network and get advice on what hiring managers will be looking for.
Friday, February 22, 2013
Zhao Wenshu on Environmental Literature in China
You are invited to attend a lecture by Dr. Zhao Wenshu, Professor and Chair of the English Department at Nanjing University in China. His talk is entitled "Teaching and Studying Environmental Literature in China."
Prof. Zhao is currently a Fulbright Scholar at the University of Illinois. He has published extensively on Chinese transnationalism, modern China, and environmental studies, including Harmony and Variation: Changing Cultural Orientations in Chinese American Literature (Nankai University Press, 2009) and "China's Peaceful Rise: A Cultural Alternative" (Boundary 2, 2006). He would also like to use the opportunity to meet with Oxy faculty/students to discuss possibilities for joint teaching/research in environmental studies in Nanjing/China.


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Cozens, C., Pinheirom V.B., Vaismanm, A., Woodgate, R., and Holligerm, P.
Notes: This paper describes a potential "specificity checkpoint" for nucleotide incorporation by DNA polymerases. Using Tgo DNA polymerase from Thermococcus gorgonarius as a model system, the authors identified a single key residue (E664) that influenced the ability to incorporate rNTPs. Mutation of E664 to lysine (K) enabled RNA polymerization in the context of another mutation (the steric-gate mutation (Y409G)). The paper describes characterization of the mutant phenotype. As part of the study, luciferase mRNA was generated from the luciferase control DNA using the mutant polymerase. Synthesis and in vitro translation of the synthesized RNA was also evaluated using ssDNA templates, New England Biolabs PURExpress™ protein synthesis kit, and the FluoroTect™ GreenLys in vitro translation labeling system. (4248)
Expand Full Notes »
Luciferase T7 Control DNA
FluoroTect™ GreenLys in vitro Translation Labeling System
Golan-Mashiach, M., Grunspan, M., Emmanuel, R., Gibbs-Bar, L., Dikstein, R., and Shapiro, E.
Notes: Specific neuronal connectivity is thought to be based on the expression of the protocadherins (Pcdh)--a family of membrane adhesion proteins. Each neuron expresses only a specific subset of the Pcdh genes. The authors of this paper used a bioinformatics approach to identify conserved, gene-specific regions upstream of the Pcdh genes. They showed that this specific sequence element (SSE) is involved in transcription regulation together with a conserved sequence element (CSE), and identified a potential interacting protein partner. To demonstrate promoter activity, the SSE-CSE region was cloned upstream of the luciferase gene in the pGL3 Basic Vector and its effect on luciferase expression was evaluated. The authors then isolated protein complexes that bound the SSE-CSE region and characterized the interacting proteins by mass spectrometry. The CCTC binding-factor (CTCF) was identified as a key molecule that binds and activates Pcdh promoters. As part of the study, human CTCF and the CTCF-binding domain were expressed in the TNT® T7 Quick Coupled Transcription/Translation System. The in vitro expressed proteins were fluorescently labeled using the FluoroTect™ GreenLys System and were used in EMSA to confirm interaction with the SSE-CSE region. (4249)
TnT® T7 Quick Coupled Transcription/Translation System
TnT® T7 Quick Coupled Transcription/Translation System, Trial Size
pGL3-Basic Vector
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Merkulova, M., Hurtado-Lorenzo, A., Hosokawa, H., Zhuang, Z., Brown, D., Ausiello, D.A., and Marshansky, V.
Notes: This study characterized interactions between the proton-pumping membrane complex V-ATPase, the Arf nucleotide binding site opener ARNO, and aldolase. The authors used a combination of protein-protein interaction techniques to identify downstream effectors of ARNO and V-ATPase signaling, and identified aldolase as a specific interaction partner of ARNO that could be involved in intracellular trafficking and cytoskeletal modulation. As part of the study, the FluoroTect™ GreenLys in vitro Translation Labeling System was used to fluorescently label recombinant proteins during in vitro translation reactions. The labeled proteins were used in pull-down experiments. (4250)
Agard, N.J., Maltby, D., and Wells, J.A.
Notes: These authors characterized inflammatory caspase substrates using an enzymatic enrichment method for caspase 1,-4 and -5 cleaved proteins and mass-spectrometry. To confirm that caspase-1 cleaved the putative substrates, some substrates were expressed and fluorescently labeled using the TNT® T7 Transcription/Translation System and the FluorTect™ GreenLys System. The proteins were then treated with recombinant caspase-1, and the progression of the reaction tracked via SDS-PAGE. The authors also used the CytoTox™ ONE Homogeneous Membrane Integrity Assay to track membrane permeabilization in relation to caspase cleavage and IL-1B release. (4252)
TnT® T7 Coupled Reticulocyte Lysate System
TnT® T7 Coupled Reticulocyte Lysate System, Trial Size
CytoTox-ONE™ Homogeneous Membrane Integrity Assay
CytoTox-ONE™ Homogeneous Membrane Integrity Assay, HTP
Hurst, R., Hook, B., Slater, M.R., Hatrnett, J., Storts, D.R., and Nath, N.
Notes: These authors compared 6 different detection strategies for protein-protein interactions on protein arrays. They expressed HaloTag® labeled bait proteins in a cell-free expression system, and captured these bait proteins onto coated glass slides using the HaloLink™ Array System. They then compared detection strategies using prey proteins labeled as follows: 1)35S methionine, 2) fluorescence (BODIPY-FL) and 3) biotin labeling of lysine residues using modified Lys tRNA, 4) chemical labeling after expression, 5) HaloTag® fusion, and 6) N-terminal FLAG tag. The authors evaluated signal:background ratios, adaptability to high-throughput screening, and ease of use. (3999)
pF1A T7 Flexi® Vector
HaloLink™ Array (TnT® SP6 Wheat Germ) Two Slide System
HaloTag® TMR Ligand
HaloTag® Biotin Ligand
TnT® SP6 Quick Coupled Transcription/Translation System, Trial Size
Transcend™ tRNA
Shibuya, N., and Nakashima, N.
Notes: The Plautia stali virus contains two open reading frames and includes a 5´ internal ribosome entry site (IRES) and an intergenic IRES region. These authors showed that the 5´ IRES was functional and initiated translation in insect cell lysate, but not in rabbit reticulocyte lysate or wheat germ extract. The efficiency of translation mediated by the 5´ IRES region was tested with and without cap analogue using various firefly and Renilla luciferase reporter constructs. They also used deletion mutants to identify the specific regions required for translation initiation. (3942)
pSP-luc+NF Fusion Vector
Talmud, P.J., Palmen, J., Putt, W., Lins, L., and Humphries, S.E.
Notes: The authors investigated common variants of the APOA5 gene that have been associated with differences in plasma triglyceride (TG) levels. PCR fragments containing either the –1131T --> C promoter variant or containing both the –1131T --> C and –3G --> A promoter variants were cloned into the pGEM®-T Vector System. The fragments were subsequently cloned into the pGL3 Basic Vector and transiently transfected into Huh7 and HepG2 cells along with the luciferase control vector, pRL-TK. The cells were lysed 48 hours after transfection and Luciferase activity was measured with the Dual-Luciferase® Reporter Assay System. The function of the 1891T --> C variant in the 3´ UTR was tested the same way; with the exception that site-directed mutagenesis was performed to introduce the T --> C at position 1891 before the fragment was cloned into the pGL3 Basic Vector. The functionality of the Kozak sequence –3A --> G variant was determined by cloning cDNAs into the pGEM®-7Zf Vector. Transcription/translation experiments were performed using the TNT® Quick Coupled Transcription/Translation System and the proteins were labeled using the FluorTect™ GreenLys System. In addition, a primer extension inhibition assay was performed using capped mRNAs generated with the Riboprobe® System –T7 and the Ribo m7G Cap Analog. Ribosome binding reactions were performed using the Rabbit Reticulocyte Lysate System, Nuclease Treated. (3460)
Dual-Luciferase® Reporter Assay System
Dual-Luciferase® Reporter Assay System 10-Pack
pGEM®-7Zf(–) Vector
pGEM®-7Zf(+) Vector
pGEM®-T Vector System I
pGEM®-T Vector System II
Rabbit Reticulocyte Lysate System, Nuclease Treated
Ribo m7G Cap Analog
Riboprobe® System-T7
Nakajima, D., Saito, K., Yamakawa, H., Kikuno, R.F., Nakayama, M., Ohara, R., Okazaki, N., Koga, H., Nagase, T. and Ohara, O.
Notes: To prepare expression-ready cDNA clones of 1589 putative full-length ORFs (from human and mouse genes) with an average size of 2.8 kb, a linear trap vector was created. Generated by PCR, this linear plasmid contained gene-specific sequences to allow homologous recombination. In addition, 5–10µg of a plasmid containing the same gene-specific sequence and the linear trap vector were purified using the Wizard® SV 96 PCR Clean-Up System. The purified DNA was resuspended in water and transformed into E. coli cells. The plasmid purified after the recombination was transferred to a Gateway expression vector and 100–200ng expressed in the TNT® T7 Quick Coupled Reticulocyte Lysate System with 0.2µl of FluoroTect™ GreenLys tRNA. The proteins expressed were resolved using SDS-PAGE. (3439)
Wizard® SV 96 PCR Clean-Up System
Suzuki, T., Kasahara, M., Yoshioka, H., Morohashi, K. and Umesono, K.
Notes: In this study, the authors investigated the interaction between the orphan receptors Dax-1 and Ad4bp/SF-1. Specific interaction was detected when the two proteins were used in both yeast and mammalian two-hybrid systems. To further characterize this interaction, an in vitro pull-down assay was performed. Dax-1 protein or a dax-1 mutant lacking an LXXLL motif were transcribed and translated in vitro using the TNT® T7 Quick System and Fluorotect™ GreenLys BODIPY labeled tRNA. The fluorescently labeled protein interacted with a Ad4BP/SF-1-maltose binding protein (MBP) fusion protein immobilized to an amylose resin. After elution and SDS-PAGE, the fluorescently labeled protein was detected using a Hitachi FMBIO® II Fluorescence Imaging System. The interaction between Dax-1 and Ad4BP/SF-1 was dependent on the LXXLL motif. (3237)
Doi N., Takashima H., Kinjo M., Sakata K., Kawahashi Y., Oishi Y., Oyama R., Miyamoto-Sato E., Sawasaki T., Endo Y. and Yanagawa H.
Notes: Template DNAs encoding c-Fos (118–211 amino acids) and c-Jun (216–318 amino acids) were generated by PCR, purified and then in vitro transcribed using the RiboMAX™ Large Scale RNA Production System. Following transcription, the RNA was purified and then translated in a wheat germ extract system supplemented with FluoroTect GreenLys tRNA. The fluorescently labeled proteins generated by the translation reaction were run on a 16.5% Tricine-SDS–PAGE and analyzed with a Molecular Imager FX. Labeling efficiency was calculated by measuring total protein via T7-antibody and determining the amount of fluorescently-labeled protein by fluorescence correlation spectroscopy (FCS). (3084)
RiboMAX™ Large Scale RNA Production System-SP6
RiboMAX™ Large Scale RNA Production System-T7
Iborra, F.J., Jackson, D.A. and Cook, P.R.
Notes: To understand if protein translation occurs in the nucleus of eukaryotic cells, the researchers in this study labeled proteins in vivo by bathing permeablized cells in a translation mix containing radiochemical, biotin or fluorescently tagged amino acids. The study found that all three types of label gave similar results. To fluorescently tag proteins, cells were grown, then permeablized, and bathed in a mix of buffer, creatine phosphokinase, phosphocreatine, GTP, tRNA, aminoacyl-tRNA synthetase, MgCl2, protease inhibitors, a 1:10 dilution of Fluorotect Greenlys BODIPY labeled lysine-tRNA complex and 50μM amino acid mixture minus lysine. The study revealed that, contrary to previous beliefs, some proteins are transcribed and translated within the nucleus in a coupled manner. (2744)
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text:
02.06.2013 /
KTM factory rider and multiple Dakar winner Marc Coma continued to occupy a top position on Sunday in Stage Two of the Sardinia Rally. At the end of the second timed special he was positioned between leader Alessandro Botturi of Italy and his old rival Cyril Despres in third. The three top riders are only separated by less than a minute.
The course took riders from Aborea to Chia on the Italian island over a distance of 292 km with the first timed special of 60 km and the second of 54 km. Sunday's stage was a marathon stage so riders were not allowed any mechanical assistance during or after the stage. This played a role in how they faced the ride, Marc Coma said.
"The first stage was very difficult with lots of navigation so I was happy to be in the lead at the end of it." Coma commented. "Then the second stage was faster but I wanted to ride carefully and take good care of the bike because it was the marathon stage."
Coma said he was satisfied with the outcome of the day's ride where the weather continued to improve and riders did not have the same muddy conditions as the previous day. "I feel very good and I am very happy with the bike. Tomorrow I need to take care of the tires, which we already used today, but this will be the same for everyone." The Sardinia Rally is a somewhat different format to other rallies and Coma rides the KTM 450 EXC Enduro machine instead of his normal KTM 450 Rally bike.
The two other KTM factory riders contesting the rally are Ruben Faria of Portugal and Jakub Przygonski of Poland. They are currently seventh and thirteenth.
Stage Three takes riders from Chia to Arborea over a distance of 293 km, which Coma expects to be faster because the ground is drying out as the weather improves. There are again two timed specials, the first of 115 km and the second of 55 km.
Results Day Two Sardinia Rally
Overall aggregate results after Sunday's two timed specials
1. Alessandro Botturi (I), Yamaha, 4:48.05 h
2. Marc Coma (E), KTM, 4:48.34
3. Cyril Despres (F), Yamaha, 4:49.03
4. Fabio Ceci (I), Yamaha, 4:52.13
5. Paolo Goncalves (P), Honda, 4:55.19
6. Joan Pedrero (E), Beta, 4:55.51
7. Ruben Faria (P), KTM, 4:56.17
8. Jordii Villadoms (E), GasGas, 4:56.19
9. Andreas Mancini (I), Beta, 4:58.54
10. Helder Rodrigues (P), Honda, 5:01.27
Other KTM
13. Jakub Przygonski (PL), KTM, 5:07.17