text:
Front Back Remote Photos (9) Video View larger Pioneer DEH-4400HD CD receiver Item #: 1304400HD 152 Reviews This item is no longer available. Original Price: $99.99 Shop all Car Stereos Need help? 1.888.955.6000 Email Chat Ask an expert advisor No pressure, no commission — just lots of good advice from our highly trained staff.
by Ken Nail If you want the best in radio sound quality, tap into the high-fidelity sound of digital HD Radio™ with Pioneer's DEH-4400HD CD receiver. Its built-in tuner lets you experience FM reception that approaches CD quality, along with the alternate programming choices offered by many stations. And this receiver really looks as good as it sounds, with a sharp dot-matrix LCD display and selectable color choices for the display and buttons. Radio's not the only thing that'll sound better It's no stretch to say that the sound of Pioneer receivers is legendary. This receiver's conservatively rated MOSFET amplifier uses special transistors to ensure strong full-range output that stands with any car stereo on the market — you'll hear solid lows and crisp highs from all your music sources. And you'll like the flexibility that the 3-band equalizer offers for dialing in just the right sound for your car and your taste. More music choices If you don't listen to the radio all the time, you'll appreciate the range of options this receiver offers. Plug an iPod® or iPhone® into the front-panel USB input and you'll be able to use your receiver's controls to zip through your songs and playlists. With a 1-amp output, the USB port will keep your device fully charged even through periods of heavy usage. You'll also find an auxiliary input, so you can plug in Dock & Play satellite radios, game consoles, and other devices. And, if you like CDs, this stereo will handle all your CDs, as well as discs or thumb drives you've loaded with MP3 and WMA files. Want to keep your steering wheel coan adapter (not included) ntrols? In most vehicles you can use an adapter (not included) to connect your vehicle's steering wheel controls to this Pioneer stereo. Connect a few wires, then program the adapter for your particular car and the Pioneer, and you'll retain the convenience of your steering wheel controls. Check the "Accessories" tab for compatible adapters. Note: If you're going to use your iPod with this receiver, be sure to check "Details" for specific iPod model compatibility with this receiver or its optional iPod adapter. Product Highlights:General features:CD receiver with AM/FM tunerbuilt-in MOSFET amplifier (14 watts RMS CEA-2006/50 peak x 4 channels)built-in HD Radio tunerplays CDs, CD-Rs, and CD-RWs, including discs loaded with MP3 and WMA files (except rights-protected files)detachable face with dot matrix LCD display, plus customizable display and key colorsremote controlAudio features:Advanced Sound Retriever (ASR) for improved sound quality from compressed music filesEasy EQ 3-band equalizer with six preset tone settings3-level loudnesslow-pass filter on preamp outputssubwoofer level controlExpandability:built in iPod control no adapter necessary, but adding Pioneer's optional connecting cable lets you leave your iPod cable at homeApp Mode offers limited control of music apps through iPhone connectioninputs: front panel auxiliary and USB inputsoutputs: 4-channel preamp outputs (2-volt front, rear/subwoofer)Other info:compatible with most factory steering wheel audio controls (adapter required)CEA-2006 compliantwarranty: 1 yearOur 60-day money-back guarantee Why replace your factory radio? Two good reasons - sound quality and cool features. A new car stereo offers power that's superior to most factory radios, along with more sophisticated tone controls. And most car stereos offer built-in or optional features that add safety and fun to life in your car, including inputs for your iPod or MP3 player, Bluetooth connectivity for hands-free cell phone calls, satellite radio or HD Radio reception, and connections for building an audio system in your car.
Not happy!! Written By NoiD, Powell WY on Saturday, March 15, 2014 Crutchfield Customer Review (What's this?) "Coolness Factor" Build Quality Ease of Use Sound Quality Value This is hard to use. If I want to play my mp3's by gender, I better pull over to do it safely. Takes too much concentration to do while driving. Speakers that were matched to play with this clip at 1/2 volume. CD player skips over railroad tracks, old Pioneer didn't. Pros: Easy to install. Cons: Purchased with (4) MTX TN502 5.25" Coaxial Speakers (5-35W). They start to clip at 1/2 volume. CD player skips. Hard to use.
Great Sounding Nissan Replacement Stereo Written By David R., Montgomery,AL on Wednesday, September 25, 2013 Crutchfield Customer Review (What's this?) "Coolness Factor" Build Quality Ease of Use Sound Quality Value Installed stereo in Nissan Quest Van.Sounds Great even with the Factory speakers. With the supplied Wiring Harnesses installation was Pretty easy. Also the HD is Great! Pros: Great Sounding Replacement at a great price. Cons: Not really any Cons to Mention.
DEH-4400HD very good buy Written By louisR, Hammond,IN on Tuesday, September 24, 2013 Crutchfield Customer Review (What's this?) "Coolness Factor" Build Quality Ease of Use Sound Quality Value DEH-4400HD 99 Dodge Dakota easy install all that came with it,matched. purchased pioneer speakers TS-675M 6.5 3 way all around sounds real good Pros: very good head unit ,not hard to figure out how to set it up,use remote makes things easy Cons: is hard to see ,but will tint rear window soon had to hold remote close to unit unlike my other pioneer from outside
excellent buy for Your money Written By Rays, Michigan on Friday, September 20, 2013 Crutchfield Customer Review (What's this?) "Coolness Factor" Build Quality Ease of Use Sound Quality Value I have had Pioneer decks before and this lives up to the standards i have come to expect from Pioneer. Pros: top notch sound quality and sound for your money, and a seperate sub control Cons: none that i have found
Great sound Written By SJT, Gloucester ri on Monday, September 02, 2013 Crutchfield Customer Review (What's this?) "Coolness Factor" Build Quality Ease of Use Sound Quality Value With this radio I can hear things I never heard before with my stock unit . Also great power Pros: Power, sound, easy hookup Cons: None as far as I'm concerned
Pioneer always Rockz Written By Siva, Maple Shade, NJ on Monday, September 02, 2013 Crutchfield Customer Review (What's this?) "Coolness Factor" Build Quality Ease of Use Sound Quality Value Pioneer rocks and the audio quality is the best whatever other brand you name it. Pros: Quality is always the best. Cons: Price is little high and I know its all about the quality but bringing the products a little lower would make you help more profit
Excellent radio for a good price! Written By Anonymous, Merrick, NY on Tuesday, August 27, 2013 Crutchfield Customer Review (What's this?) "Coolness Factor" Build Quality Ease of Use Sound Quality Value This was very easy to install with the harness that was provided for my type of car. I would def recommend this site for all audio equipment...


text:
Open Monday through Friday 8:30 am–5 pmSaturday 10 am – 4 pm & Sunday 1 pm – 4 pm
Asian-inspired gifts, beautiful scarves, jewelry and a selection of Pearl S. Buck's books are just a few of the items available. The International Gift Shop is managed and lovingly maintained by the Pearl S. Buck Volunteer Association. Members of Pearl S. Buck International receive a 10% discount on all items. Become a member today.

subset: commoncrawl


text:
Dec 9, 2012 9:17 AM
Venom 14 u
Like (0)
Fast growing team looking for 2 to 3 players due to injury. Venom is out of Northwest Indiana and is a well jelled team with no drama!! We are looking for a starting catcher,infielder, and a outfielder. Interested, give me a call.... My name is Dave phone # is 219-808-6959 you wont be disappointed!!!
What positions are you still looking to feel.
Online Golf Tee Times with Last Minute Tee Time Deals & Discounts
Cool Running is the complete online resource for runners.
Find your favorite campground from the best campgrounds listing!


text:
Product Information
Object | Take apart and put back together. |
Difficulty | Level 9 - Gruelling |
Brand | Pelikan |
Types | Yavuz Demirhan, European Wood Puzzles |
Dimensions | 7.8 cm x 5.1 x 5.1 cm / 3.1 in x 2 in x 2 in |
Packaging | Cardboard Box |
Product Description
This puzzle was crafted in Europe with absolutely fantastic quality.
Each of the puzzles of Pelikan design are unparalleled in their class!
Every piece of the puzzle is precisely cut and very well made; you cannot find its equal anywhere else.
The diversity in wood makes each puzzle a unique masterpiece.
The Burrito is a 5 piece burr cube designed by Turkish designer Yavuz Demirhan.
Customer Reviews |
Write your own review!
1 out of 5 stars
Disclaimer: These reviews are written by our customers. Puzzle Master makes every attempt to keep these posts informative and relevant. If you find a post objectionable or inappropriate, please let us know by sending an email to firstname.lastname@example.com
On Thursday January 23rd Stephen Pitt-Francis wrote,
I've just received a bunch of five puzzles, the others are good but this one is a real let down. I got it as it was a '9' but I dont know why its a 9 as its painfully easy! You need no thought its so obvious. Spend your money on something else on here!


text:
Top Definition
Weapon or gun that shoots plastic or small metal pallets.It is usually used by kids or teens trying to look tough. Most of them look like real guns so it can be used for bluffing.
If we are in trouble at the party, draw out the bb gun.
додав piebeeallin
27 Січень 2008
4 more definitions
someting that if not used under parental supervision can result in lots of hurtness
ouch the damn bb gun hurt me again
додав lalalaicanthearyou
14 Вересень 2003
bb gun: A type of gun that shoots bbs and/or pellets. It is not a firearm but it can be as fast as 1000 FPS. bb guns are commonly used for competition and target shooting. Lots of teenagers think they look cool by shooting it because some look like real guns. They use it to shoot windows and other peoples property just to be ass holes. If you get one threat it like a real gun. Be safe.
My brother and i both got bb guns to target shoot.
додав sniper21
11 Червень 2010
A device resembling a traditional firearm, that when fired is indicative that the operator is victorious in his chosen endeavor. For it's inverse please see Apple Sauce.
We won! We Won! We shot that BB Gun!
додав DJ Felony
7 Березень 2009
The name of the back seat on the passenger's side.
If it's a coupe, it's the trunk. If someone already called shotgun, you're fucked.
If it's a coupe, it's the trunk. If someone already called shotgun, you're fucked.
James: I call shotgun!
Nick: I call pistol!
Tom: Dammit, I call bb gun.
Nick: I call pistol!
Tom: Dammit, I call bb gun.
додав Eric Cardona
12 Жовтень 2014


text:
save up to 50%*
$10 off your next purchase of $50 or more. Register Now
Columbus Day Sale - 20% Off All Sweaters* Shop Now
$ 395.00
These cuff links are set in sterling silver and crafted by hand in Birmingham, home of England's world-famous jewelry corner. The unique, vibrant color is magnificently deepened by a technique of vitreous enameling, which hard fires colored glass for a beautifully resilient finish. Sterling silver base. Together these offer substantial weight along with a timeless sense of style. Made in England.
Unfortunately, we are unable to fulfill your requested quantity at this time. The remaining items will be shipped as soon as they are available.


text:
Jim Riggleman says that, as of now, Jason Marquis will make his next scheduled start. The Nationals manager, though, couched that declaration pretty strongly, suggesting several times this morning that Marquis' rotation spot is not at all secure.
"If he's progressing and getting more action on the ball, getting the ball down more, getting more groundballs, then certainly we'll see that things are moving in the right direction," Riggleman said. "But we can't just let him be out there and get his brains beat out, either."
Marquis, who allowed five runs in four innings last night and actually lowered his ERA to 14.33, is on pace to start Saturday in Philadelphia. But it wouldn't be difficult for the Natioanls to make a change. Tomorrow's day off provides some leeway. The expected arrival very soon of both Jordan Zimmermann and Yunesky Maya also provides a couple of starting alternatives.
Zimmermann starts today for Class AAA Syracuse against Indianapolis. Maya, who tossed three hitless innings in his Gulf Coast League debut Friday, will probably make one more start in Viera before advancing to a higher-level affiliate.
If Marquis (who, of course, is on a two-year, $15 million contract) was removed from the rotation, he'd almost certainly remain on the major-league roster, possibly in the bullpen. Rosters expand in 16Read more »
- Todd Boss - Aug 15, 2010 at 5:38 PM
What does it matter, really, at this point in the season if Marquis does go out and get shelled each night. He's obviously trying to work his way back to shape. We're in last place, playing for draft position at this point. If his losses get us the 6th pick versus the (current) 9th pick then so be it. I don't mean to sound defeatist but, in a season where we're obviously looking to see who gives us the best chance to win in 2011, shouldn't we be actually throwing Marquis over and over to see where he stands? Yeah I want to see Maya and JZimmermann but I also want to know what I should do with my most expensive pitcher next year.
- Doc - Aug 15, 2010 at 5:40 PM
"We made a commitment to Jason….". It seems that with Riggleman it's more about what the Nats can do for Marquis, not vise-versa.
- Grandstander - Aug 15, 2010 at 5:57 PM
I think he said that more as a, "we're stuck with this asshat for another year so we've got to at least try and get him to be able to do something for us" rather than a "we promised we would start him" kind of thing.
- Anonymous - Aug 15, 2010 at 7:25 PM
"Well, I'm a woman. I can change my mind," Riggleman joked.Alert all the idiots who just got done having their panties in a twist over what Dibble said that it's time for them to put on a new pair.
- Dryw - Aug 15, 2010 at 9:30 PM
Yeah, I was thinking that Rigg's comment was a lot closer to actually being sexist….and as a card-carrying woman, I will openly admit to being completely NOT offended by either comment.
- phil dunn - Aug 15, 2010 at 9:32 PM
My advice, don't buy a ticket for a game Marquis is starting unless you want to see a game that is guaranteed to be over by the second inning.
- Suz - Aug 15, 2010 at 10:16 PM
As a female full STH, I am in full support of Riggs getting in touch with his feminine side if doing so will deliver us from watching Marquis start another game until after No. 21 has well and truly figured out what the heck is wrong. Sausage making and Marquis's rehabbing are two processes best not done in public.
- JaneB - Aug 15, 2010 at 10:21 PM
Todd, I'm with you.
- natsfan1a - Aug 16, 2010 at 1:57 AM
Oh, geez. Now Riggs will have to issue an apology, too. Oh, the humanity.(And, seriously. I'm not offended by that one, either. ;-))
- Sec3MySofa - Aug 16, 2010 at 3:30 AM
If the Cubs, who aren't going anywhere interesting this year, either, can send Zambrano to the pen, and then to Iowa, I think we shouldn't need to watch Marquis's career twist in the breeze like this, here.
- natsfan1a - Aug 16, 2010 at 1:01 PM
One more thought on the offensive front (again, from a female point of view), while I was not offended by the comments of Dibble or Riggleman, I'm not crazy about the use of a term for a feminine hygiene apparatus as a pejorative statement.* After all, use of said apparatus is based on the idea that a naturally self-cleansing female body part is dirty and in need of rinsing out.My humble suggestion, if you're really all that worried about gender stereotyping, maybe y'all should use a more gender-neutral term, such as – oh, I don't know – enema bag, when you feel the need to hurl insults at someone with whom you disagree. Just a friendly suggestion. ;-)—*Usage example: An anti-Dibble blog link that currently appears on the right side of this page.
- Anonymous - Aug 16, 2010 at 3:52 PM
"*Usage example: An anti-Dibble blog link that currently appears on the right side of this page."Yeah, you do have to wonder why Zuckerman gives prime sidebar space to someone like Needham who has a history of making offensive posts in his blog. (Witness the one that got him kicked off NBCWashington.com last year.) I'm pretty sure that neither the WaPo or MASN would allow the d-bag word to even appear in a comment to their blogs, but here Zuckerman is with it right there on the front page thanks to his link to Needham.


text:
Brittany's singing with her 2 Igglybuff(I don't know the spelling of their names), well it will ace a high score for attraction to the mob of adult men. The song Brock sang in the stage, "Brock's Paradise", well, it's a nice one, but he repeated it again and again and again tiringly, therefore it looked boring to the audience. I might be the next one to learn the lyrics of that song.......... (Sorry, TR. Your singing of your motto doesn't look nice to the audience.)
If you're interested in dance songs, and you're running itunes (much safer than limewire or those russian sites, and it supports the artists who make the music)
check this link -- the "euro club hits" series:
This series the "Euro Club Hits" I think has some real winners on it.....
it was a good episode but the singing was awfull, it's not my taste so that was a downer for me, still it's a nice episode so i'll give it a 8/10.
Reminder from August 09, 2010- Name my future Reshiram "Griffin"...
My Current HeartGold Team:
If you want, look at Yu-Gi-Oh! C,Pokiman, and Naruto C, but they haven't exactly been updated recently...
STILL REJECTING PMs. SORRY. (No offense to anyone.)
Didn't have much use for the girl, but I almost laughed myself sick at Brock's song. Who didn't have their minds try to go places they really didn't need to when he started singing about his "happy place"?
I know! I was pleasantly surprised to hear her singing voice ^_^
Ehh... sorta funny, but not the highlight for me.Wobbufett strutted it's stuff which had gotten rare. It's still funny that this patient Pokemon hold all of TR's wins against the twerps since "Princess vs. Princess".
Brock's Paradise is one of my favorite songs for that very reason Not to mention his ridiculous getup just makes it that much funnier."Brock's Paradise" (the dubbed version) finally appeared and it was funny that it's only song that he knows and nearly passes out. His voice is so bad it only adds to the comedy.
All in all, a decent episode, I would say.
lord freeza i really need to use the space skype
I loved all of the singing in this episode. I thought Brittany was a great singer. I thought that Brock's singing was okay. However, his outfit amused me. I thought that the Igglybuff were just adorable and I loved seeing them dance in the background. I also liked how the narrator fell asleep by Jigglypuff's song along with everyone else.
I noticed a mistake that when Misty sends out her Corsola, she throws a regular Poke Ball instead of a Lure Ball
I <3 May.
I do not do competitive battling and I really do not have any desire to. I also do not go out of my way seeking for shinies because I never really understood why they were so great anyways. All I truely enjoy pokemon contests, breeding, and in-game battling.
I think grass pokemon are the greatest ever and I take great pride in the ones I have raised. I also think that Chikorita is the most adorable pokemon ever!
If you would like to know me send me a message. I'm not shy(:
I remember this episode from 6 years ago. It was cool. Brittany was awesome in this episode and I really enjoyed her songs (Though I couldn't admit it back then).
Well this was a decent episode.I liked Brittanys singing,and whole concept of two igglybuff being stars.They were debuted in different way than other pokemon during Johto,and it was also good seeing good old Jigglybuff again and his dropouts of jealousy.
Another good thing was definitely Brock entertaining audience with his famous "Brocks Paradise" song,while Ash,Misty and Brittany went to save Igglybuffs from Team Rocket.Though negative thing was that he sang only one song all the time,which resulted with audience being fed up.
All in all while its not one of my favorites,this episode had its moments.
Episode great! Her singings great and her Igglybuffs are great! Brock was really funny in the jap ending! Its called "Brock's Paradise"
Im giving credit to Pokesho
We Are The Champions!
Good filler.
The Igglybuffs were cute. and i loved Brittany, cool songs.
Wobbuffet and his trainer.(credit: Serebii.net)
The rocket song was cool, too bad that they never used it again after this.
Not a too bad episode. Loved the way Jigglypuff hit the two Igglybuff twins and then pretended she was helping them That scene actually makes this episode good lol
Watching these episodes in order btw so that's why I have posted in aloty of threads
Boy, Japanese singers seem way better than english singers after listening to this episode. Igglybuff are cute but I wonder how come they don't put people to sleep unlike Jigglypuff does.
My ideal battle ever!
This episode was ok... It was cool seeing Igglybuff for the 1st time. The Igglybuff were really cool! I didn't like how jealous Jigglypuff was acting towards them. The song Brock performed was not fun to listen to, I didn't like it...
Not a bad episode. Cool to see Igglybuff for the firsty time.
Pokemon HeartGold team:
I really like this episode quite a lot. The Igglybuff were cute, and Jigglypuff made an awesome appearance. And let's not forget that Brock was great as well! And Ash used Noctowl, which was also nice, it's even rare if Noctowl pops holes in balloons, it's so rarely used... Anyways, 8/10.
Author's Profile
Aquatic Passion (multi-chaptered fic)
Misty, The Mysterious Mermaid (oneshot)
Together Forever (drabble collection)
Love, South Park Style (oneshot)
Working On A Dream (multi-chaptered fic)
Merry Christmas (oneshot)
Seaking Catching Day (oneshot)
Awareness (multi-chaptered fic)
Nice episode overall, I liked it.
I just wish TR used their song more than twice. :[
Mexican Brock ftw!!!! That is probably the most hilarious thing I remember from Pokémon and I was ecstatic when I saw they did that again in Battle Frontier. It was also nice to see Jigglypuff again too. This episode can be basically summed up as an hilarious episode with lots of singing
This is one of the better Jigglypuff episodes. It's pre-evolved form, Igglybuff, is pretty cute too.
Ceci n'est pas une signature
Though I die, La Resistance lives on


text:
Several MLSers talk about their favorite player which happens to hold a special place in our hearts, Brian McBride.
Now that the month of April is over who is your player of the month?
Choose between rookie Aaron Schoenfeld working his way into the starting lineup, veteran Eddie Gaven for his two goals against Houston, Josh Williams making his first two MLS starts, Chad Marshall anchoring the backline and Danny O'Rourke coming back from injury to stabalize the midfield.
FREE Showing of the Jay Demerit Story at Capital University on Friday, April 27th at 7:00pm.
Rise and Shine follows the inspirational journey of Jay DeMerit from the 9th division of English football to the World Cup. It is a terrific story and one that every player & fan will love! ALSO..... Jay will be in town with the Whitecaps to play the Crew the next night and if his travel plans allow he may stop by the screening.
To RSVP your FREE tickets please go to the following link and click the Reserve ticket link in the middle of our page:
The Crew make their first appearance in 2012 on national TV this Saturday on the new NBC Sports channel. The Black & Gold travel to Philadelphia to take on the Union Saturday at 3:30pm.
Click here to find NBC Sports on your local provider.
Official Crew Pub Tour stop this week at BBR in the Arena District. Learn More
FOX Sports Ohio color analyst and long time Crew player Duncan Oughton previews the matchup between the Crew and the New York Red Bulls. Hear his take on the matchup and how the Crew can stop the Red Bulls attack.
Eric Gehrig reflects on his Man of the Match honors in Toronto:


text:
As good as it gets! Our flagship collection delivers the ultimate in music and home theater performance with stunning aesthetics.
Looking for archived products?
Click here
Paradigm accessories are meant to enhance the award-winning music and home theater experience delivered by Paradigm speakers.
For use with: PV-60R, CS-60R, CS-60R-SM, AMS-150RX, AMS-150R-SM, AMS-150RX-SM, SA-15R, SA-15R-SM,...


text:
The court upheld a ruling from the lower U.S. District Court for Alaska. The appeals court said Shell had shown "a likelihood of success on the merits of its claim that Greenpeace USA would commit tortuous or illegal acts against Shell's arctic drilling operations in the absence of an injunction," reports energy news site Rigzone.
Shell charged that Greenpeace activists used illegal means to interfere with their arctic drilling campaign.
Greenpeace campaigners in 2010 boarded Shell's rig Harvey Explorer in the Gulf of Mexico. It was slated for later arctic work. In February 2012, the group employed similar tactics with the Noble Explorer drillship as it prepared to leave New Zealand for Alaskan waters.
Shell's drilling program in the Beaufort and Chukchi seas last year was hampered by equipment failures and weather-related delays. Its drillship Kulluk struck ground off the coast of Alaska in January.
U.S. Greenpeace Executive Director Phil Radford said Shell's decision to delay work offshore Alaska amounts to an admission that those echoing concerns about oil and natural gas exploration in arctic waters were right.
There was no public response Thursday from Greenpeace on the court ruling.


text:
Several new lightning fires but firefighters are keeping them small
According to a statement released by the Plumas National Forest there is lots of smoke in the air but just a handful of very small fires resulted from the last lightning storms moving through the Plumas National Forest on Wednesday night and early Thursday morning (August 21-22). The majority of the fires were on the eastern portion of the forest.
With the exception of the Chance fire above Frenchman Lake (3.6 acres and holding), the remaining fires are controlled or contained. The majority of those were 1/10 - 1/4 acre or smaller. The attached Beckwourth Ranger District map shows the latest fires and other lightning starts, also contained/controlled from earlier in the week (Bagley, Creek, Old House & Mallard).
Several other small fires similar in size to those above were picked up on the Mt. Hough and Feather River Ranger Districts. All are contained, controlled or close to it.
The fires remaining active but also partially contained include the Johnson, Ridge, and Heights in the Mt. Hough Complex. More specific information about them may be found on inciweb.org; look for Plumas Lightning on the dropdown menu.
Most of the smoke, particularly on the eastern part of the forest, is traveling up from the Rim fire in the southern Sierra.
More fires may crop up as the moisture from the thunderstorms dissipates.


text:
Bank holidays, free museums, the footy, street markets, smart sarcasm, Sunday roasts, real ale pubs, pop-ups, curry, tea and cake, the NHS, minding the gap
The weather, high rents, rising tube fares, queues, commute times, slow walkers, the NHS, politicians and bankers, people who stand on the left
Explore 48 London neighborhoods. Which are right for you?
Find a neighborhood


text:
Tips on Buying Lamp Shades
It's easy to spruce up old lighting fixtures with the right lamp shades for your home. Whether you need to refresh your table lamps or make reading lamps more inviting, you'll probably need a bit of information before you buy lamp shades that will work with your lamps and your decor. Read on for tips on buying lamp shades.


text:
I have been lazy the last couple of years. I have a couple of projects to work on but family and other obligations have taken me away. No More!!!
1) Fix cracked turtleback of my 32nd P Type XXIII.
2) Work on Argonaut Jr. model. So far hull is cut out and portholes are done. Still need frames etc.
How about you???!!!!


text:
Skip to main content
If you are required to submit a claim form for pharmacy benefits, your pharmacist can provide you with the National Drug Code that you should include on the claim form.
Contribute an Insurance LetterSubmit a successful insurance letter to our template letters collection and help other PHers overcome insurance challenges!
Browse our collection and learn more about submitting a letter.
Stay informed about current research, events, and more.
Sign up


text:
Focused on the highest standards of technical Magento Development
We keep a tight focus on our core speciality rather than being a jack of all trades full service agency
Our unique selling point is our high level of focus on the technical aspects of building, updating and fixing a web site. This comes under the general heading of "web development".
In addition to this it's worth mentioning that we have a deep understanding and make regular use of the Git version control system and are also happy to use Subversion, Mercurial or any other system that your project might already be using. Version control is an important part of keeping control of a software development project. Often the first thing to do when diagnosing any kind of code issue is to simply look at the git logs.
Our clients include a range of e-commerce business, digital agencies and more who trust us to handle or supplement their technical development requirements
Checkout Down, Disaster Recovery or Critical Bug Fix - We can Help, Today
We will thoroughly examine your project or website for Security, Performance and Code Quality issues and Provide you with Recommended Solutions
There are all kinds of good reasons to get your project thoroughly audited by an impartial third party. Our audit service will provide you with a list of recommended improvements and will highlight any issues and concerns that we find.
Open or Closed Book PHP Development Outsourcing and Support to Assist you with your Client Projects
We are a small team of UK based developers, here to help you.
Based in the heart of West Yorkshire in the UK, Edmonds Commerce are a small team of skilled developers focusing on PHP and e-commerce. We work together as a team and are carefully managed and nurtured by Nolwenn, our Managing Director.
Talk to us about your New Project, Support or Consultation and Audit requirements


text:
Book your room at Fairfield Inn & Suites by Marriott Charlotte Uptown
Room, 1 King Bed (Guest)
Max Occupancy: 3 guests (up to 2 children, 2 infants)Room, 2 Queen Beds, Balcony (Guest)
Max Occupancy: 4 guests (up to 3 children, 3 infants)Room, 1 King Bed, Balcony (Guest)
Max Occupancy: 3 guests (up to 2 children, 2 infants)Studio King
Max Occupancy: 4 guests (up to 3 children, 3 infants)Room, 2 Queen Beds (Guest)
Max Occupancy: 4 guests (up to 3 children, 3 infants)Family-friendly Charlotte hotel in Charlotte Center City, near Time Warner Cable Arena
Fairfield Inn & Suites by Marriott Charlotte Uptown is centrally located in Charlotte, walking distance from Marshall Park and close to Afro-American Cultural Center.
This family-friendly hotel is within close proximity of Actor's Theatre of Charlotte and American Legion Memorial Stadium.
Make yourself at home in one of the 193 air-conditioned rooms featuring microwaves. Rooms have private balconies. Complimentary wired and wireless Internet access is available. Conveniences include coffee/tea makers and irons/ironing boards, as well as phones with free local calls.
Enjoy a range of recreational amenities, including an outdoor pool, a fitness center, and a seasonal outdoor pool. This hotel also features complimentary wireless Internet access, gift shops/newsstands, and complimentary use of a nearby fitness facility. Guests can catch a ride on the complimentary shuttle, which operates within 3 mi.
Grab a bite to eat at the hotel's restaurant, where you can take in a pool view, or stay in and take advantage of room service (during limited hours). Quench your thirst with your favorite drink at a bar/lounge.
Featured amenities include a business center, a computer station, and express check-out. Planning an event in Charlotte? This hotel has 10000 square feet (900 square meters) of space consisting of a conference center, conference space, and meeting rooms. Self parking (subject to charges) is available onsite.
Information missing or incorrect? Tell us!Renovations & Closures
- Bar/lounge
- Dining venue
- Fitness facilities
- Swimming pool
Hotel Amenities
Hotel Amenities
In addition to an outdoor pool, Fairfield Inn & Suites by Marriott Charlotte Uptown provides a fitness center and a seasonal outdoor pool. A bar/lounge is on site where guests can unwind with a drink. A complimentary breakfast is offered each morning. A computer station is located on site and high-speed wireless Internet access is complimentary.
Business-related amenities at this 3-star property consist of a business center and meeting rooms. Event facilities measuring 10000 square feet (900 square meters) include a conference center and conference space. This family-friendly hotel also offers gift shops/newsstands, coffee/tea in the lobby, and complimentary newspapers in the lobby. A complimentary area shuttle within 3 mi is available for guests. Onsite parking is available (surcharge).
Fairfield Inn & Suites by Marriott Charlotte Uptown is a smoke-free property.
- 24-hour front desk
- Total number of rooms - 193
- Number of floors - 11
- ATM/banking
- Bar/lounge
- Business center
- Coffee/tea in lobby
- Free newspapers in lobby
- Free breakfast
- Conference space
- Dry cleaning/laundry service
- Express check-out
- Fitness facilities
- Gift shops or newsstand
- Free WiFi
- Computer station
- Outdoor pool
- Laundry facilities
- Meeting rooms
- Porter/bellhop
- Luggage storage
- Safe-deposit box at front desk
- Free area shuttle
- Conference space size (feet) - 10000
- Conference space size (meters) - 900
- Conference center
- Self parking (surcharge)
- Smoke-free property
- Number of buildings/towers - 1
- Outdoor seasonal pool
- Free use of nearby fitness center
Available in all rooms: Free WiFi , Free wired high-speed Internet
Available in some public areas: Free WiFi
Self parking (surcharge)
Room Amenities
- Balcony
- Air conditioning
- Climate control
- Phone
- Free local calls
- Coffee/tea maker
- Microwave
- Daily housekeeping
- Hair dryer
- Iron/ironing board
- Television
- Cribs/infant beds available
- Free WiFi
- Free wired high-speed Internet
- Room service (limited hours)
- Down comforter
- Individually furnished
Where to Eat
A complimentary hot/cold buffet breakfast is served each morning between 7 AM and 10 AM.
Q Tavern - Overlooking the pool, this pub serves lunch and dinner. Open daily.
Room service (during limited hours) is available.
Nearby Things to Do
An outdoor pool and a seasonal outdoor pool are on site. Other recreational amenities include a fitness center.
To make requests for specific accessibility needs, complete the "Special or Accessibility Requests" section of "Trip Preferences" when you book your hotel.
- Accessible bathroom
- Accessible path of travel
- Braille or raised signage
- Accessible parking
- In-room accessibility
- Roll-in shower
Hotel Policies
Check-in time starts at 3 PM
Check-out time is noon
Payment types
You need to know
Extra-person charges may apply and vary depending on hotel policy.
Government-issued photo identification and a credit card or cash deposit are required at check-in for incidental charges.
Special requests are subject to availability upon check-in and may incur additional charges. Special requests cannot be guaranteed.
- Self parking fee: USD 15 per day


text:
Kernel and DRM was written by David Airlie.
Don't knot who wrote DRI but it was probably Keith Packard cuz hes awesome!
GEM was written by Intel. Then someone wrote TTM.
Wayland was written by Kristian Høgsberg.
Gallium3D was written by Tungsten Graphics who got bought by VMware.
Xorg was written by Keith Packard.
So what the fuck did AMD do all this time?
Marek Olsak had a large part in getting the r300g/r600g driver working. Alex Deucher helps with radeon kernel code. Tom Stellard has been making good progress with OpenCL. The most disappointing thing (to me) is that Christian Konig's work on video acceleration has seemingly been held up by legal issues.
You should read more Phoronix articles and comment on less of them.
true but AMD only promised documentation and basic proof of concept code, they never promised they will develop the driver itself.
even so they have hired some good devs from the community to help, example the initial SI mesa patch among other stuff.
and obviously AMD provide support for the devs in case they have questions or doubts about internals of the GPU ASM API.
i agree in the fact they could help a bit more but is still way better than what nvidia do tho
* as i understand it(maybe im wrong) even when cairo in theory is not part of the stack per se it has received many patches in these years since wayland/weston use it heavily for the testbed and example code among other stuff
* you get me wrong here im not referering to an specific driver/GPU, im talking about theorical graphic stack capabilities and in this case linux new graphic stack is getting very close in features to win7 and in some cases is fairly superior
* well for my desktop PC user r600g is perfectly fine and 100% usable and well having an 1200W PSU is not like few watts more are going to ruin my day, aka
-- kwin with gl working uber
-- xonotic at ultra setting for clear the mind works peachy and fast enough at 60ish FPS
-- firefox/chromium webgl and render accel works very good
-- mplayer/ffmpeg MT and my phenom II X4 965 handle 1080p 60 5.1 like a champ with Xv, so im not desperated for UVD(i expect my AMD FX 6200 to improve it more after make world with gcc 4.7)
-- blender works peachy for my hobbists designs
-- Lineage2 Tauti/AION in wine 1.5.6 + r600g is peachy here too. i could use some more FPS in sieges but the engine in lineage2 sucks ass even on win7
so im happy with r600g in my specific use case scenario, so for now "it sucks" depending on your needs
hmm ok... informative comments in this thread... about the video-capabilities... yes it woudl be great to have some uvd like feature... I have here 2 zacate pcs... one htpc/server and one notebook... on both I get not always fluent results with 720p x264 video files... so that sucks a bit...
to redhat I am very glad that they do good job here especialy with the amd drivers... but fedora has some issues... I even wrote a bug report... so I cant use it ^^ it seems a response to such a bug report takes long... so maybe off-topic but maye a amd guy is here ^^
its a known bug btw, but seems to interest nobody at all for a long time...strange that its distribution specific thought that that should be happen very seldom because all use the same upstream...
but I should maybe debug it by myself, just trying other kernels other alsa versions... but I am not shure that I migrate form ubuntu to fedora anyway ^^ so I have no big interest in invest so much time ^^...
ok but that gets to off-topic...
is there any very ruff prognosis when some kind of video acceleration for the radeon driver could hit mesa or gallium3d?
I would be happy about that... then maybe better powermanagement but even that is not that important... because that kind of semiworks ^^ and have not very big issues with that... I dont cant think anything thats so important than this feature.... yes some guys need more 3d speed or better opencl support but at least the first of it would be way harder to get the driver to nearly the same speed as the closed ones fast... but uvd like feature would make so much people happy...


text:
Little Car Show provides big value
UPDATED 7:21 AM MDT Aug 16, 2012A little car show gives auto enthusiasts the chance to see unique cars for free.
The views expressed are not those of this site, this station or its affiliated companies. By posting your comments you agree to accept our terms of use.blog comments powered by Disqus


text: MAC Refined Golden Bronzing Powder ($24.00 for 0.35 oz.) is described as a "finely spun golden with soft pearl finish." It's a medium-dark tan brown with warm undertones–a little red, a little yellow–and gold sparkle. This is the darkest/deepest bronzer of the launch, so for more than just a warm tint on deeper complexions, this would be your best bet (though you might consider other shades within the permanent range). This is like a darker version of MAC Soft Sand. Urban Decay Toasted is more red-toned.


text:
Play Bray welcomes Katie home Colman O'Sullivan reports from Bray as the town welcomed home Katie Taylor
Play Official Team Ireland Homecoming Eileen Whelan and George Hamilton present live coverage of the official Irish Olympic Team homecoming in Dublin city centre
Play Interview: Ireland's Olympic Medallists Marty Morrissey speaks to Ireland's five Olympic medallists as they arrive in Dublin airport
Play John Joe Nevin Interview Silver medallist John Joe Nevin tells RTÉ's Caitríona Perry that he enjoyed the whole experience of London 012
Play Bronze Boys home in Belfast Dave Kelly catches up with Paddy Barnes and Michael Conlan as they arrive home in Belfast
Play Darragh on Cian O'Connor Darragh Maloney says the Irish people will be able to see Cian O'Connor in action all over the country
Play Ireland homecoming: Report Joe Mag Raollaigh reports from Dublin airport as the Irish team arrive home
Play "It's great to be home" - Billy Walsh Billy Walsh tells Marty Morrissey that the Olympics were physically and mentally demanding and it is great to be home
Play Team Ireland return home Eileen Dunne is joined by Mick Dowling, Tom Freyne, Michael Carruth and Des Cahill to welcome home the Irish Olympic team.
Play Ireland Homecoming: Press Conference Watch the press conference to welcome home team Ireland at Dublin airport
Play Natalya's ninth an "incredible achievement" Hugh Forde tells Darragh Maloney that Natalya Coyle's ninth place finish in the Modern Pentathlon is an incredible achievement
Play Billy and the Bronze Boys Paddy Barnes, Michael Conlan and Billy Walsh join Darragh Maloney to reflect on the boxing success at the London Olympics
Play The Agonies and the Ecstasies! A look back at the highs and lows of the London Olympics 2012 from an Irish perspective as heard on RTÉ Radio 1.
Play Interview: Walsh and Conlan Billy Walsh and Michael Conlan join Con Murphy in the wake of Michael medal ceremony
Play Games Review: Stephen Martin Stephen Martin, Chief Executive of the OCI, gives Damien O'Meara his opinion on how the games have gone for Ireland
Play "Boxers getting it right" - Taoiseach Taoiseach Enda Kenny tells Roy Willoughby that the boxers have got it right in terms of strategy, planning and access to technical expertise
Play "Difficult" marathon for Kenneally Ireland's Mark Kenneally, who finished in 57th place, says he found the last few kilometers of the marathon very difficult.
Play Kenneally hoping for better in Rio Mark Kenneally tells Jacqui Hurley that he hopes to be in much better position to put in a better performance in Rio 2016


text:
HEAT oven to 350°F.
PLACE chicken, top sides down, on work surface; spread with mustard. Top with ham and cheese. Roll up tightly, starting at one short end of each.
DIP chicken in egg product, then roll in bread crumbs until evenly coated on all sides. Place, seam sides down, in 13x9-inch baking dish sprayed with cooking spray.
BAKE 40 to 45 min. or until chicken is done (165°F), covering after 25 min.


text:
What's New:
Complete Business Management
With integrated CRM, projects, billing, sales & marketing, reporting and collaboration, 1CRM covers all the bases to ensure that you only need one software solution to manage your entire business.
One System - Many Industries
Whether you work in manufacturing, high tech, retail, or real estate, 1CRM is the custom-fit solution you've been looking for. Adapting to any industry with its uniquely flexible design, 1CRM saves time and helps you do a better job.
Work from Anywhere
Securely access your data anytime, anywhere, and from any Windows, Mac, iOS, Android or Windows Phone device. Bring remote teams together effectively.
1CRM is an open & customization friendly CRM that you can customize to suit your business needs. Modify your screen and print layouts, menu system, dropdown options, and user interface theme & colours. Create multiple personalized dashboards and a custom mobile experience! Take your CRM to the next level with your own custom modules, drag 'n drop custom print layouts, and 18 types of custom fields!PERSONALIZED CRM
1CRM features CRM solutions for every budget. From the free Startup Edition download to the advanced Enterprise Edition, there are no forced upsells and always clear pricing.
Don't need many users? No problem! With either On-Premise Software or the 1CRM Cloud Service, you can start with just 1 user and add more as needed. Wary of long term commitments? Use 1CRM Cloud month to month! Just getting started? Use 1CRM Cloud Startup Edition for only $8/user/month!
Try 1CRM for 30 Days – no credit card required


text:
Subscribe Now eEdition Login How to Advertise Customer Service Contact Us
dellrapidsinfo.com Weather Jobs Cars Homes Apartments Shopping Classifieds Calendar
Pastor's Column: Mouth guard
(Psalm 141:3)You've probably seen athletes wearing a mouth guard while they play their sport. It keeps them from injuring their mouth, breaking teeth, or biting through their tongue while in the heat of competition. The mouth guard can save someone a lot of pain. I especially like it when a player wears a mouth guard that's a golden yellow color because it makes him look like he has "corn teeth". That looks pretty cool, in my opinion. Maybe his Mom isn't really in favor of such a mouth guard since it looks like her son hasn't had very good tooth-brushing habits, which would reflect negatively on Mom. Did you know that the Bible talks about using a mouth guard? In Psalm 141:3 we read: "Set a guard over my mouth, O'Lord; keep watch over the door of my lips." David compares our mouth to an open door or gate, and of course the guard would be someone to watch what comes out of that gate. Posting a guard over the mouth gate could definitely save us plenty of pain. Our 30th President, Calvin Coolidge was a man of very few words. During a time when people thought that prosperity was here to stay, Coolidge was very fiscally conservative, and also vocally conservative. People admired his thriftiness with money and words, even though they had no intention of following Coolidge's example. One of Coolidge's famous quotes was: "I never had to take back what I didn't say." It sounds like he and David understood what the LORD wants all of us to practice; keep a guard over our lips. Once we've said something, it's impossible to unsay it. We can't take it back, try as we might; we can be forgiven, but the words are out there, for better or for worse. Of course, there are also times when we should speak up; pray for God's wisdom in knowing when to speak and when to keep silent. Either way, it will save us a lot of pain, just like a mouth guard.
Argus Leader
Dell Rapids Public School
Dell Rapids St. Mary's Catholic School
Dell Rapids Chamber of Commerce
City of Dell Rapids


text:
Font size A A A
Enter up to 3 words to generate multiple domain name combinations. Tick the box for the words you want the thesaurus to use. Good words to try are first, quality, great etc because they generate a lot of good results.
Use thesaurus
The domain name thesaurus is potentially one of the best tools for somebody looking for a domain name.
If you have a fixed business name, you could add a word in front or after your business name describing your business and select thesaurus on this word.
If you do not have a fixed business name, then this should show many different combinations of available domain names.
Domain Name Thesaurus We hope the 'Domain Name Thesaurus' tool helps. If you have any suggestions, please contact us
We hope the 'Domain Name Thesaurus' tool helps. If you have any suggestions, please contact us
We're hoping soon to bring some pages on ecommerce, email, web hosting, dedicated servers, virtual servers, cloud servers, website analytics, merchant accounts, web design services, affiliate programs, ssl certificates, domain auctions, domain parking, logo design


text:
Valley Yarns
FILTERValley Yarns knitting/crochet patterns and weaving drafts are for personal use only and may not be copied. Any commercial use, including selling patterns and/or drafts and items made from them is prohibited.
Commonly used abbreviations in Valley Yarns patterns can be found here.
Webs Discount
- 20% Off
- On highlighted items totaling $60 or more!
- 25% Off
- On highlighted items totaling $120 or more!


text:
Would usb to serial work too?
I'm not sure how it is on r600g, but I remember reading that it was working too. Not all GL3 stuff works, obviously.
I'll be glad when Texture Compression, etc are enabled by default.
But until then these little posts about performance:
a. Are always subject to debate by the people who have enabled the hidden tweaks the rest of the userbase has not a single clue about. Which leads me to suspect one of us needs to go to Ubuntu Brainstorm and add a proposal to have the tweaks enabled by default. Texture compression, odds and ends, filesystem modifiers
b. Should actually be run from fluxbox so that limited resources are used and we see the raw results.
c. I'm actually very happy running LTS copies of the operating system with one exception. 8.04 / 8.10 were the prime for my laptop in terms of raw performance. Although I'm neglecting the I915 driver. Ubuntu 11.04 is the prime for that chipset. See you can't have your cake and eat it too.
d. Distributions need a build system that incorporates these performance exceptions by compiling mismatches in terms of New Xorg with Old userbase. New userbase with Old Xorg. Puppy is about the only distribution I've read about who does this.
But who am I, what do I know of such things. I'm but a sentinel watching the progress of slime sliding down a dirt hill.
Fluxbox is getting compositing this gsoc, you can't then say it brings the best performance
Does anyone play Quake Live with the radeon driver? It's about the only game I play that is a "must work". I don't own an ATI card so I can't test it myself.


text:
There are no user reviews for this movie. Be the first to review this movie!
Be the first to rate this movie!
Tom Cruise & The Ultimate Director Co-Pilots What's Tom Cruise's key to Hollywood success? Over the course of 30+ years, the industry's top gun has played for an A-list roster of filmmakers that's unmatched in showbiz history. Take a look.


text:
To find a doctor, call 800-667-5356 or click below:
Find a Doctor
Request an Appointment
En Español (Spanish Version)
Definition | Reasons for Procedure | Possible Complications | What to Expect | Getting Autopsy Results
An autopsy is a surgical procedure to examine the body and its internal organs after death.
An autopsy is not done after every death. An autopsy may be done at the request of the family or doctor. Reasons for autopsy include:
Autopsies may not be able to provide exact answers on cause of death.
Before an autopsy, there must be positive identification of the body. An autopsy permit must be signed by the legal next-of-kin. The body is transported to the morgue and held in a refrigeration unit until the autopsy.
Autopsies follow this general procedure:
Copyright © Nucleus Medical Media, Inc.
The body is sewn back together after the autopsy is complete. Procedures vary regarding organ replacement. Dissected organs may be returned to the body or incinerated. If the organs are not returned to the body, the mortician will put filler in the body cavity to retain the body's shape.
Tissue samples may be sent to a lab for analysis. Results are available within a few weeks. A final autopsy report is usually completed in 30 to 60 days.
The autopsy typically takes 2-4 hours, depending on the reason and level of complexity.
Contact your local Medical Examiner's office on how to request an autopsy report.


text:
Don't Have an Account? Join the IHG® Rewards Club
Redeem when you want, for what you want.
Reward Nights When redeeming points for a Reward Night, you may only book one room at a time. Once you complete your Reward Night reservation you will be given the option to duplicate this reservation.
* Requires affiliation in the respective organization.
** Best Available Rate Search does not include affiliation rates.
To book more than 9 rooms for one stay or request a quote, please use our Easy Meetings tool, call 1-800-MEETING (in United States, Mexico and Canada) or contact a Global Sales Office.
Located off Interstate 75, exit 228, 15 minutes south of Atlanta Hartsfield - Jackson International Airport, the Stockbridge Holiday Inn Hotel & Suites is one of the premier hotels on the south side of Atlanta. Whether you are an executive always on the run, or just stopping over on your journey to another destination, our staff is here to provide you with an unprecedented level of service.
Our new Holiday Inn Hotel & View More
Our new Holiday Inn Hotel & Suites Stockbridge - Atlanta has a 5-story, spectacular atrium lobby offering many areas for casual gatherings. Our On the Roxx Bistro Restaurant offers carefree dining just off the Atrium. If you're not in the mood to go out for dinner, room service is available until 10 pm. Or, you may want to stop by On the Roxx Lounge, our Atrium Lobby Bar for cocktails and to catch up on what's happening with the latest news in sports. Or you can simply relax in one of our well-appointed 2-room suites, complete with two flat screen TVs.
If a special event or corporate meeting is in your plans, our conference facility will accommodate up to 40 people, and a Boardroom seats up to 12 people. Additionally, in-house catering is available, with a wide array of menu choices.
The next time your travel plans call for a hotel in the Atlanta metro area or Stockbridge, GA, give us a call. We promise to provide you with the best in Southern hospitality at our Stockbridge, GA hotel!
Quality Excellence Award
Second only to the Torchbearer Awards, The Quality Excellence Awards are created to honor hotels who have maintained high standards in product quality, hotel operation and customer satisfaction as measured by our guests


text:
18" Adult School Stack Chair - 5 Pack - Various Colors
by ECR4Kids
Item #: 248161
Model #:
Price includes shipping
- $ 165 98 165.98
Choose a color:
Please select a color
- Shipping
- Price includes shipping
- Enter ZIP Code for shipping options
Delivery estimates, taxes, and fees are based on ZIP Code. Club Pickup orders are based on your club's current price and item availability on the day of payment.
About this item
These innovative 18" school stack chairs feature scratch-resistant chrome legs. Choose from seven available colors.
The molded seats with vented backs have reinforced ribbing in back and under seat for strength. These chairs also feature 14 gauge tubular steel legs and steel lower back support with full MIG welded frames. No hardware penetrates the seat side shell, eliminating clothing snags and injury.
- Chrome-plated steel legs can be sanitized, will not chip or flake and are scratch resistant, unlike painted legs
- Steel tubular legs are stronger and offer greater stability than many competitors' tapered legs
- One piece molded seat with vented back and reinforced ribbing underneath provides comfort and strength
- Vented chair back allows air to circulate to the body
- No hardware penetrates the seat side shell, eliminating clothing snags and injury
- Full MIG welded frame for sturdiness, as opposed to weaker spot welding
- Inter-stack with most other manufacturers' stack chairs
- Color scheme blends with classroom furniture made by many other manufacturers
- Meets or exceeds ASTM F963 safety standards
Assembled Country
Component Country
USA And Imported


text:
We're here to help you explore your options and find the right school for your child. To get started with the school research process, check out the resources below to learn more about how to choose a school and the schools available in your area.
Imagining your ideal school »Let others know what you think about your school
Live in San Francisco? We did the homework to help you find the very best school for your child.
Learn More »In an exclusive adaptation from her new book, "The Smartest Kids in the World," Amanda Ripley encapsulates her three years studying high-performing schools around the globe into a few powerful guidelines.
Learn More »Summer planning for kids is tricky: how's a parent to find top-notch camps and classes sight unseen? These expert tips help separate the best summer programs from the rest.
Learn More »Our mission is to help millions of parents get a great education for their kids. GreatSchools.org is an independent nonprofit and the leading national source of school information for families.
GreatSchools.org 1999 Harrison Street, Suite 1100, Oakland, CA 94612
©1998-2015 GreatSchools.org All Rights Reserved. GreatSchools is a 501(c)(3) not-for-profit organization | Ad Choices


text:
SKU: memphis_memories_shoot_collection_dvd
DVD-R Version
Please verify you have a compatible player before purchasing our DVD-R titles. We are not responsible for the compatibility of your player to our DVD-R titles. Click here to check: (DVD-R Compatibility Chart)
A special edition of Shoot Interviews featuring Memphis wrestling stars! This collection includes 5 discs with complete interviews of such greats as Jerry "The King" Lawler, "Superstar" Bill Dundee, Lance Russell, Gypsy Joe, and Ronnie P. Gossett!
Relive their great histories and hear their great stories from their careers in pro wrestling. This is a tremendous deal to pick up FIVE great shoot interviews for one low price!
BONUS OFFER:You're getting the Memphis Shoot Collection, why not add some Classic Memphis Wrestling to go with it? Add any of the Classic Memphis DVDs for $12.99 each or get all three and save big! Add all the newly released Classic Memphis Wrestling DVDs (Vol. 1, 2 & 3), all THREEFOR ONLY $30!!! You save 23% off the regular price!!!
Classic Memphis Wrestling bonus offer includes:


text:
Noisebridge Distilled
Contents |
Noisebrige Distilled
This is about people who want to learn the art of building, maintaining, and/or operating a still
Sean is planning on teaching a hands-on class on distilling basics, but needs to decide a time! Vote for your favorite below. You can vote more than once. Total time for the entire class will probably be about 3-4 hours or so since physics is a slow mistress. A basic outline of the class is as follows:
- Overview of what distilling is, and why it works
- Things you can distill, and what you can expect out of the other side
- Intro to the still, and what all the crazy valves do
- Post- and pre- processing operations, and why your really do need to do them (a.k.a. Brita filters are useful)
- And of course...hands on use of the still, and a chance to try the fruits of your labor!
Wednesday, January 5th @ 7PM
- Namez
Tuesday, January 14th @ 7PM
- Namez
Wednesday, January 15th @ 7PM
- Namez
The Equipment
For the class, a 20L custom designed glass still will be used since its a great teaching aid (you can actually see what is happening!) It can handle both reflux and pot distillation modes with just a couple of minor adjustments (if you don't know what that means, but want to find out, you should come to the class!). However, I'll cover how to "roll your own" out of a variety of different materials.
Owning and operation of a still is legal in California as given by part 23367 of the ABC code. Full unabridged (read: annoying) text of the 2010 ABC code can be found here: [1]
Note: Still laws vary state to state, so check to make sure you aren't breaking any laws!


text:
This collection of stories are set after the wedding of Ron and Hermione and feature the Potters, and the Weasleys as two (happily?) married couples.
Parent Series: None
Categories: Remains of the Day > Eternity
Characters: Ginny Weasley, Harry Potter, Hermione Granger, Ron Weasley
Genres: Action/Adventure, Angst, Comedy, Drama, Fluff, General, Mystery, Romance
Time Period: Post-War
Warnings: None
Challenges: None
Challenges: None
Open: Closed [Report This]


text:
Drugs A - Z
Antitussive with Expectorants Oral syrup
It is used to treat cough and congestion
Generic Name: dextromethorphan/guaifenesin/phenylephrine
Brand Names:
Execof Er, Robitussin Pediatric Cough & Cold Cf, Duravent Dm, Tusso-Xr, Gfn 1200/Dm 20/Pe 40, Dexcon-Pe, Tussex, Robitussin Children'S Cough & Cold Cf, Neotuss Improved, Dacex-Pe, See more brands »
What is this medicine?
DEXTROMETHORPHAN, GUAIFENESIN, and PHENYLEPHRINE (dex troe meth OR fan, gwye FEN e sin, fen il EF rin) is a combination of a cough suppressant, an expectorant, and a decongestant. It is used to treat cough and congestion. This medicine will not treat an infection.What should I tell my health care provider before I take this medicine?
They need to know if you have any of these conditions:- diabetes
- enlarged prostrate
- glaucoma
- heart disease
- high blood pressure
- if you have taken an MAOI like Carbex, Eldepryl, Marplan, Nardil, or Parnate in last 14 days
- lung or breathing disease, like asthma
- thyroid disease
- an unusual or allergic reaction to dextromethorphan, guaifenesin, phenylephrine, other medicines, foods, dyes, or preservatives
- pregnant or trying to get pregnant
- breast-feeding
How should I use this medicine?
Take this medicine by mouth with a full glass of water. Follow the directions on the prescription label. Take with food or milk. Use a specially marked spoon or container to measure your dose. Household spoons are not accurate. Take your medicine at regular intervals. Do not take it more often than directed.Talk to your pediatrician regarding the use of this medicine in children. While this drug may be prescribed for children as young as 4 years old for selected conditions, precautions do apply.
Patients over 60 years old may have a stronger reaction and need a smaller dose.
What if I miss a dose?
If you miss a dose, take it as soon as you can. If it is almost time for your next dose, take only that dose. Do not take double or extra doses.What may interact with this medicine?
Do not take this medicine with any of the following medications:- ergot alkaloids like dihydroergotamine
- MAOIs like Carbex, Eldepryl, Marplan, Nardil, and Parnate
- stimulant medicines for attention disorders, weight loss, or to stay awake
This medicine may also interact with the following medications:
- alcohol
- barbiturates like phenobarbital
- certain heart medicines like metoprolol, digoxin, and methyldopa
- diuretics
- doxazosin
- medicines for depression, anxiety, or psychotic disturbances
- medicines for sleep
- medicines to treat blood pressure
- muscle relaxers
- other medicines for cold, cough, or allergy
- some medicines used for sleep during surgery
- tamsulosin
What should I watch for while using this medicine?
Talk to your doctor if your cough lasts for more than 1 week. If you have a high fever, skin rash, lasting headache, or sore throat, see your doctor.Drink several glasses of water each day. This will help loosen mucus.
You may get drowsy or dizzy. Do not drive, use machinery, or do anything that needs mental alertness until you know how this medicine affects you. Do not stand or sit up quickly, especially if you are an older patient. This reduces the risk of dizzy or fainting spells. Alcohol may interfere with the effect of this medicine. Avoid alcoholic drinks.
What side effects may I notice from receiving this medicine?
Side effects that you should report to your doctor or health care professional as soon as possible:- allergic reactions like skin rash, itching or hives, swelling of the face, lips, or tongue
- anxiety, irritable, or excited
- breathing problems
- confusion
- fast, irregular heartbeat
- seizures
- tremor
- unusually weak or tired
Side effects that usually do not require medical attention (report to your doctor or health care professional if they continue or are bothersome):
- diarrhea
- dizziness
- headache
- nausea, vomiting
- stomach upset
- tiredness
- trouble sleeping
The Healthline Site, its content, such as text, graphics, images, search results,
and other material contained on the Healthline Site ("Content"), its services, and any information or material
posted on the Healthline Site by third parties are provided for informational purposes only. None of the
foregoing is a substitute for professional medical advice, examination, diagnosis, or treatment. Always seek
the advice of a physician or other qualified healthcare provider with any questions you may have regarding a
medical condition. Never disregard professional medical advice or delay in seeking it because of something you
have read on the Healthline Site. If you think you may have a medical emergency, call your doctor or 911 immediately.
Please read the Terms of Service
for more information regarding use of the Healthline Site.
Copyright © 2005 - 2015 Healthline Networks, Inc. All rights reserved for Healthline. Healthline is for informational purposes and should not be considered medical advice, diagnosis or treatment recommendations. Terms of Use | Privacy Policy


text:
Related Regions: Liechtenstein, Triesenberg
2.9 2 Resort Reviews Write New Review
Malbun - Steg FL
Login to Facebook Connect to post a commentPlease limit responses to 250 characters.
View All
Malbun makes special efforts for children. More
Mobile | E-mail | Facebook | Twitter | Google+
Click to collapse
Search for resorts in:
Click the map to add a state to your resort search
Click to add Colorado


text:
Don't die on us, buddy.
Don't die on us, buddy.
"It's not enough that I succeed, others should fail."
Sponsored Links |
BTW, there was a great quote during the Knicks' post game show.
"Carmelo owns more of the Nets than Jay-Z". LOL
Last edited by MetsFanatic19; 12-19-2012 at 11:05 PM.
Sponsored Links |
How ****ing fantastic. My school is gonna be on lockdown because of an alleged shooting plan.
"It's not enough that I succeed, others should fail."
No I mean a shooting is supposed to happen tomorrow.
Ah this is kind of funny.
"It's not enough that I succeed, others should fail."
Good thing I'm watching Elf in Trig class and Finding Nemo in Marine Studies . Something to make the day go faster.
Just got back from my basketball game. Probably the most exciting one I've played in my entire life.
Goes back and forth the entire game. Overall, we played very poorly and should've killed them, but we've never seen a 1-3-1 zone before and really **** the bed.
So we're down by 7 with 1 minute left in the 4th. We go down, hit a 3. 45-41. We run a full court zone press - get a steal and an easy layup. 45-43. With 10 seconds left, their player misses both free throws and after the time out, we get it inside to our big man (around 6'5" with red hair, lol). With 1 second left, he gets fouled and time expires. He hits both free throws and we go to over time.
Same thing in over time, back and forth. This time, we're down by 4 with 30 seconds. We go down, hit a huge three after what should've been a reaching in foul. We get the ball back down by 1 with 6 seconds left and get it inside again. Once again, he gets fouled and time expires.
He hits both free throws again and we win in overtime. It was insane. Last year, we went 0-14 in league play. This year, we're 2-1 (should be 3-0) and only lost by 2 to the best team in the league (we were up by 10 at half and we didn't get a call for us in the 2nd half).
Winning really feels good.
Sponsored Links |


text:
Language: English Continue
This country code will remain if no action is taken to change it.
Our website uses functional cookies that do not collect any personal information or track your browsing activity. When you select your country, you agree that we can place these functional cookies on your device.
Sort By: Descending Journal Year Ascending Journal Year
Inoue, H., Hayashi, N., Matsushita, A., Xinqiong, L., Nakayama, A., Sugano, S., Jiang, C.J. and Takatsuji, H.
Notes: To understand the mechanism of Panicle blast 1 (Pb1) gene-mediated resistance to rice blast, a rice fungal disease, researchers investigated Pb1 interacted with a transcription factor involved in resistance, WRKY45 that is regulated by the ubiquitin system. To study how these proteins interacted, inner rice leaf sheaths were bombarded with gold particles coated with 0.5 µg of effector plasmid, 0.3 µg of NanoLuc® luciferase reporter and 0.1 µg of reference Renilla luciferase. After incubating overnight at 28°C, samples were ground in liquid nitrogen and reporter activities assayed using the Dual-Glo® Luciferase Reporter Assay System and Nano-Glo® Luciferase Assay System. The Renilla luciferase gene was also split into an N-terminal construct and C-terminal construct, expressed in rice protoplasts and assayed for reconstituted Renilla luciferase activity. Expression was normalized to firefly luciferase. (4510)
Expand Full Notes »
Nano-Glo® Luciferase Assay
Dual-Glo® Luciferase Assay System
pGL4.70[hRluc] Vector
Expand Products »
Siesser, P.F., Motolese, M., Walker, M.P., Goldfarb, D., Gewain, K., Yan, F., Kulikauskas, R.M., Chien, A.J., Wordeman, L. and Major, M.B.
Notes: To better understand what roles FAM123A may play in signaling, cell behavior and human disease, HT1080 sarcoma cells were plated on MatTek dishes coated with 5mg/ml fibronectin before transfection with Venus (a yellow fluorescent protein)-tagged FAM123A or Venus-WTX, another member of the FAM123 gene family, using FuGENE® HD Transfection Reagent. The cells were imaged the next day for low-resolution analysis. For a higher magnification, dynamic analysis, HT1080 cells were plated onto Delta T dishes coated with 5mg/ml fibronectin and transfected with EGFP-FAM123A and FuGENE® HD Transfection Reagent. The next day, cell images were captured every 10 seconds. Examining the effect of silencing FAM123A, a reporter assay used the pGL4.34[luc2P/SRFRE/Hygro] Vector cotransfected with a CMV-Renilla coreporter and other effector plasmids or siRNA, and luciferase levels assessed using the Dual-Glo® Luciferase Assay System. (4246)
FuGENE® HD Transfection Reagent
pGL4.34[luc2P/SRF-RE/Hygro] Vector
Keyaerts, M., Remory, I., Caveliers, V., Breckpot, K., Bos, T.J., Poelaert, J., Bossuyt, A., and Lahoutte, T.
Notes: These authors investigated the effects of various anesthetics on bioluminescence imaging with firefly luciferase. They observed decreases in luminescence with volatile anaethetics, and found increased luciferase expression with injectable anaethetics in intact cells, but not in cell lysates in vitro. They concluded that the decreases in luciferase activity observed with volatile anaesthesia were due to hemodynamic effects, and not due to a direct inhibitory effect on luciferase enzyme itself. The apparent enhancement of luciferase activity with certain injectable anaesthetics appeared to be due to cytotoxic effects that resulted in increased permeablity to luciferase, as the same enhancement was not observed in cell lysates. D-Luciferin was used for in vivo imaging experiments. The pGL4.10 vector (encoding firefly luciferase), Luciferase Assay Reagent, and the GloMax® 96 Microplate Luminometer were used for in vitro assays using cell lysates. (4190)
Luciferase Assay Reagent
GloMax® 96 Microplate Luminometer
pGL4.10[luc2] Vector
VivoGlo™ Luciferin, In Vivo Grade
Cock-Rada, A.M., Medjkane, S., Janski, N., Yousfi, N., Perichon, M., Chaussepied, M., Chluba, J., Langsley, G., and Weitzman, J.B.
Notes: These authors investigated the role of matrix metalloproteinase (MMP-9) in a reversible model of cancer that is initiated by infection with intracellular Theileria parasites. They found that gene induction by parasite infection was associated with trimethylation of histone H3K4 (H3K4me3) at the MMP-9 promoter. The H3K4 methyltransferase SMYD3 was the only histone methyltransferase upregulated upon infection. They therefore investigated the role of SMYD3 overexpression on MMP-9 expression and cell migration, identifying SMYD3 as an important new regulator of MMP-9 transcription. During the study they used the GloMax® Multi Luminometer to measure luminescence and absorbance in reporter and cell viability assays. They also used the Dual-Luciferase® Reporter Assay to measure SMYD3 activity in cells transfected with a SMYD3 reporter, and the pGL4-hRluc/TK plasmid for normalization of the experimental reporter activity. GoTaq® DNA polymerase was used in semi-quantitative RT-PCR. (4189)
Dual-Luciferase® Reporter Assay System 10-Pack
pGL4.74[hRluc/TK] Vector
GloMax®-Multi Luminescence Module
GoTaq® DNA Polymerase
Xie, Y., Rosser, J.M., Thompson, T.L., Boeke, J.D., and Wenfeng, A.
Notes: This paper describes a rapid dual-luciferase-based assay for L1 retrotransposition that is amenable to high-throughput screening. A firefly luciferase vector in which the luciferase gene was disrupted by an antisense intron was constructed by introducing a 900-bp fragment of the human γ-globin intron into pGL4.13. This Fluc gene, interrupted by an antisense intron, gives only minimal luciferase expression unless the luciferase gene is restored by a retrotransposition event. The authors also tested a similar retrotransposition reporter using the pGL4.73 Renilla luciferase vector, but found that the firefly construct gave much higher signals. They therefore used the firefly luciferase retrotransposition reporter, a Renilla luciferase normalization control and the Dual-Luciferase® Assay to characterize profiles of retrotransposition by various human and mouse L1 elements, and to measure the kinetics of L1 retrotransposition in cultured cells. The GloMax® Multi Luminometer was used to quantify luciferase activity. (4205)
Dual-Luciferase® Reporter Assay System
GloMax®-Multi+ Detection System Base Instrument with Shaking
GloMax®-Multi+ Luminescence Module
GloMax®-Multi Base Instrument
pGL4.13[luc2/SV40] Vector
pGL4.73[hRluc/SV40] Vector
Linker, R.A., Lee, D-H., Ryan, S., van Dam, A.M., Conrad, R., Bista, P., Zeng, W., Hronowsky, X., Buko, A., Chollate, S., Ellrichmann, G., Brück, Dawson, K., Goelz, S., Wiese, S., Scannevin, R.H., Lukashev, M. and Gold, R.
Notes: The authors of this paper investigated the molecular mechanisms through which fumaric acid esters affect the progression and pathology of multiple sclerosis. The authors looked at the activation of the Nrf2 transcriptional pathway which plays a role in defense against oxidative stress. A stable reporter cell line expressing eight copies of the gluthione-S-transferase 2 antioxidative response elements cloned upstream of the luciferase complementary DNA in a pGL4.26 vector. Reporter cells were stimulated with dimethylfumarate for 24 hours and luciferase activity measured using the Luciferase Assay System. A strong dose-dependent induction of antioxidative response elements was observed. Next, the authors looked at the effects of fumaric acid esters on the inhibitor of Nrf2, Keap 1. C-terminal, V5-tagged Keap1 protein was transfected into 293 cells. Forty-eight hours post transfection, cells were treated with dimethyl fumarate. Samples were lysed, Keap1-V5 was immunoprecipitated and gel purified. The protein was digested with trypsin in the presence of ProteaseMAX™ Surfactant, Trypsin Enhancer. Peptide pools were analyzed using liquid chromatography-tandem mass spectrometry. The authors were able to show that the Keap1 protein was modified in response to treatment. (4215)
ProteaseMAX™ Surfactant, Trypsin Enhancer
Luciferase Assay System
pGL4.26[luc2/minP/Hygro] Vector
Sedlák, D. Paguio, A. and Bartůněk, P.
Notes: pGL4.35 and pGL4.36 reporter vectors were used. (4264)
pGL4.35[luc2P/9XGAL4UAS/Hygro] Vector
pGL4.36[luc2P/MMTV/Hygro] Vector
Paguio, P., Stecha, P., Wood, K.V. and Fan. F
Notes: pGL4.15[luc2P /Hygro] or pGL4.14 [luc2/Hygro] were used. (4265)
pGL4.14[luc2/Hygro] Vector
pGL4.15[luc2P/Hygro] Vector
Ghisolfi, L., Calastretti, A., Franzi, S., Canti, G., Donnini, M., Capaccioli, S., Nicolin, A. and Bevilacqua, A.
Notes: To examine post-transcriptional regulation of B cell lymphoma (bcl)-2 mRNA in human cell lines, the bcl-2 open reading frame was cloned into the pCI-neo Mammalian Expression Vector with a FLAG tag. This construct was transfected into U2OS human osteosarcoma cells, lysed, immunoprecipitated on Anti-FLAG-coated beads and analyzed by SDS-PAGE. A 260bp fragment containing the human c-myc 3´UTR adenine-uridine(AU)-rich element (ARE) was cloned into the pGL4.71 [hRlucP] Vector above to produce the pGL4.71PmARE plasmid. For transient expression, SK-N-BE and HEK293 cells in 96-well plates were cotransfected with 200ng of the pGL4.71 constructs (carrying c-myc or bcl-2 ARE) and 200ng of the pGL3-Control Vector. Luciferase expression was assessed using the Dual-Glo® Luciferase Reporter Assay System. HEK293 cells were also stably cotransfected with the same pGL4.71 constructs and a vector carrying G418 resistance. Renilla luciferase expression was assessed and clones chosen with similar expression levels. (4071)
pCI-neo Mammalian Expression Vector
pGL4.71[hRlucP] Vector
Nagase, T., Yamakawa, H., Tadokoro, S., Nakajima, D., Inoue, S., Yamaguchi, K., Itokawa, Y., Kikuno, R.F., Koga, H. and Ohara, O.
Notes: These authors used the Flexi® Vector System to prepare ORF clones encoding 1929 human genes and to transfer a subset of these clones to various expression vectors for further analysis. They created HaloTag® fusion proteins and examined expression of these proteins in vitro and in COS7 and HEK293 cells. They also performed comparisons between the Flexi® System and Gateway® cloning system, specifically examining the effects of flanking sequences on protein expression in in vitro translation systems and confirming that the cellular localization of the HaloTag® fusion proteins was consistent with results obtained using GFP-fusions. (3800)
Anti-HaloTag® pAb
Flexi® System, Entry/Transfer
Flexi® System, Transfer
GloMax® 96 Microplate Luminometer w/Dual Injectors
Monster Green® Fluorescent Protein phMGFP Vector
pF1K T7 Flexi® Vector
T7 RiboMAX™ Express Large Scale RNA Production System
TnT® SP6 High-Yield Wheat Germ Protein Expression System
Wizard® SV 96 PCR Clean-Up System
Belov, G.A., Altan-Bonnet, N., Kovtunovych, G., Jackson, C.L., Lippincott-Schwartz, J. and Ehrenfeld, E.
Notes: In this study, the Renilla luciferase gene from the phRL-CMV Vector was incorporated into polioviral RNA in place of a structural protein-coding region. Renilla luciferase activity was then monitored as a convenient indicator of viral replication. Specifically, these authors tested whether the guanine nucleotide exchange factors GBF1 and BIG2 could rescue brefeldin A (BFA)-induced inhibition of polioviral replication in HeLa cells. Cells were transfected with plasmids encoding either GBF1 or BIG2, together with the firefly-luciferase-encoding pGL4.13 Vector, which was used as a control to monitor transfection efficiency. Eighteen hours post-transfection, the cells were transfected with polioviral replicon RNA containing the Renilla luciferase gene. One hour later, normal growth medium containing EnduRen® Live Cell Substrate and either BFA or solvent alone, was added and Renilla luciferase activity was monitored at hourly intervals for 7 hours. Cells were then lysed and firefly luciferase activity was measured to assess transfection efficiency using the Dual-Glo® Luciferase Assay System System. In the presence of BFA, GBF1 was able to partially rescue viral replication. In the presence of BIG2 and BFA, no viral replication occurred. (3562)
EnduRen™ Live Cell Substrate
Tanaka, M., Chock, P.B., and Stadtman, E.R.
Notes: Oxidative damage has been associated with a range of age-related neurological conditions. In this study, the effect of mRNA oxidation was investigated. A direct correlation was observed between the extent of oxidation and the frequency of translation errors. The authors excised the firefly luciferase (luc2) gene from the pGL4.14 Vector, attached a FLAG tag to the 5´ terminus and a Myc tag to the 3´ terminus, and subcloned the gene into a pGEM-4Z Vector that had been modified to append a poly(A) sequence. The construct was transfected into HEK293 cells, which were then cultured in the presence of an oxidizing agent. The occurrence of truncated protein fragments and short peptides increased in the presence of the oxidizing agent in a concentration-dependent manner. The effects of oxidation of mRNA were also investigated in in vitro translation experiments using mRNA treated with an iron-ascorbate mixture and hydrogen peroxide. Translation in vitro was performed using rabbit reticulocyte lysate supplemented with protease inhibitors. The translation products were detected using anti-FLAG and anti-c-Myc antibodies. (3630)
pGEM®-4Z Vector
Rabbit Reticulocyte Lysate System, Nuclease Treated
Campbell, D.B., Sufcliffe, J.S., Ebert, P.J., Militerni, R., Bravaccio, C., Trillo, S., Elia, M., Schneider, C., Melmed, R., Sacco, R., Persico, A.M. and Levitt, P.
Notes: In this family-based study of 1,231 autism cases, a genetic association of a common C allele in the promoter of the MET gene and autism was identified. Initial sequencing of the MET genes from 86 individuals with autism was used to identify several candidate variants in the MET promoter and 3´ untranslated region. Family-based analyses were then performed to determine whether an association could be demonstrated between any of these variants and autism. A G/C variant 20bp 5´ of the MET transcription start site was found to be overrepresented among individuals with autism, particularly among families where more than on child was affected. Once the candidate variant was identified, the effect of the G/C change on transcription of the MET gene was investigated in a reporter assay. Two 762 bp fragments of the MET promoter region, differing only in the G/C nucleotide, were cloned into pGL4.10 firefly luciferase reporter vectors. These vectors were then transfected into mouse neural cell lines, and luciferase production was monitored using the Dual-Glo™ Assay. The construct containing the C allele produced less than half of the luciferase activity of construct containing the G allele. (3579)
Budhram-Mahadeo, V.S., Bowen, S., Lee, S., Perez-Sanchez, C., Ensor, E., Morris, P.J. and Latchman, D.S.
Notes: Previously, the POU domain of Brn-3a was shown to interact with p53 and increase cell survival. In this article, the authors explored the possibility that Brn-3b, which shares a POU domain 95% identical to Brn-3a, may interact with p53 and affect its role in apoptosis. To test the protein:protein interaction, GST-Brn-3b fusion protein was bound to glutathione Sepharose beads and incubated with 35S-methionine labeled full-length or truncated p53, prepared using the TNT® T7 rabbit reticulocyte lysate. The luciferase control included in the kit was used as the noninteracting protein control. After washing, the bound proteins were resolved by 12% SDS-PAGE, and the bands examined by radiography. To examine the effect of Brn-3b on two p53-regulated genes, Bax and p21cip1/waf1, ND7 cells were transiently transfected with empty vector, Brn-3b, Brn-3a or p53, or Brn-3 with p53. The reporter gene cotransfected was under the control of wildtype Bax, wildtype p21cip1/waf1, or mutant Bax. A control vector (Renilla luciferase driven by the thymidine kinase promoter) was used for normalization. Forty-eight hours posttransfection, the cells were harvested and reporter levels assessed using the Dual-Luciferase® Reporter Assay System. (3597)
TnT® T7 Quick Coupled Transcription/Translation System, Trial Size
pRL-TK Vector
TnT® T7 Coupled Reticulocyte Lysate System
TnT® T7 Coupled Reticulocyte Lysate System, Trial Size
TnT® T7 Quick Coupled Transcription/Translation System
Dual-Luciferase® Reporter 1000 Assay System
Novak, A.J., Grote, D.M., Ziesmer, S.C., Kline, M.P., Manske, M.K., Slager, S., Witzig, T.E., Shanafelt, T., Call, T.G., Kay, N.E., Jelinek, D.F., Cerhan, J.R., Gross, J.A., Harder, B., Dillon, S.R. and Ansell, S.M.
Notes: To test the significance of the C or T polymorphism at position -871 of the serum B-lymphocyte stimulator (BLyS) promoter region, the BLyS promoter was amplified, digested with Kpn I and cloned into the pGL3-Enhancer Vector. HL60 cells were then transiently transfected by electroporation using 10µg of the pGL3-BLyS-promoter constructs and 40ng of the pGL4.75[hRluc/CMV] Vector, which expresses Renilla luciferase. After 48 hours, expression of the reporter genes was assessed using the Dual-Luciferase® Reporter Assay System. (3343)
pGL3-Enhancer Vector
pGL4.75[hRluc/CMV] Vector
Liau, S-S, Jazag, A. and Whang, E.
Notes: The HMGA1 proteins are known to play an important role in transcriptional regulation of human gene expression, and are overexpressed in several malignancies. This study investigated the role of HMGA1 in cellular invasiveness and metastasis of pancreatic adenocarcinoma. Overexpression of HMGA1 in the human pancreatic cell line MiaPaCa2 resulted in increased invasiveness in a Matrigel assay, and shRNA silencing of HMGA1 expression resulted in reduced invasiveness and reduced the activity of matrix metalloproteinase-9 (MMP-9), an important mediator of malignant cell invasiveness. The authors then used a reporter assay to investigate the effect of changes in HMGA1 expression on MMP-9 activity. Vectors either overexpressing HMGA1, expressing an shRNA directed against HMGA1, or expressing a control shRNA, were cotransfected into MiaPaCa2 cells along with a pGL4.12 firefly luciferase reporter vector construct containing the MMP-9 promoter upstream of the luciferase gene. MMP-9 promoter activity was significantly decreased upon HMGA1 silencing. The authors then showed that HMGA1 silencing resulted in decreased invasiveness and reduced tumor growth and MMP-9 activity in an in vivo model. (3578)
pGL4.12[luc2CP] Vector
Vinther, J., Hedegaard, M.M., Gardner, P.P., Andersen, J.S. and Arctander, P.
Notes: These authors used stable-isotope labeling of amino acids in culture (SILAC), a method that allows quantitation of relative protein abundance between populations, to investigate the effect of the microRNA miRNA-1 on the HeLa cell proteome. HeLa cells grown in the presence of labeled arginine and lysine were transfected with miRNA-1, and the labeled proteins compared to those from control cells treated with the transfection reagent alone. A set of 16 proteins repressed by miRNA-1 was identified. The 3´ UTR's from 11 of the miRNA-1-regulated genes were tested in a reporter assay, and 6 were shown to repress expression in an miRNA-1-dependent fashion. For the reporter assays, the HSV TK promoter was amplified from the pRL-TK Vector and subcloned into the pGL4.12(luc2CP) Vector, creating the pGL4.12-TK vector. The 3´ UTR regions from suspected target genes were then amplified and subcloned into the pGL4.12-TK construct. The various pGL4.12-TK constructs (0.9µg) were then co-transfected along with pRL-TK (0.1µg) and miRNA-1 (50pmol) into HeLa cells (80,000 cells/well in 12-well plates). Twenty-two hours post-transfection, firefly and Renilla luciferase activities were measured using the Dual-Luciferase® Reporter Assay System. (3561)
Hu, X., Z., Lipsky, R.H., Zhu, G., Akhtar, L.A., Taubman, J., Greenberg, B.D., Xu, K., Arnold, P.D., Richter, M.A., Kennedy, J.L., Murphy, D.L., and Goldman, D.
Notes: Three alleles were identified in the promoter of the serotonin transporter, HTTLPR, based on the number of a specific repeat sequence and a single nucleotide polymorphism found in the repeat sequence. Sequences from these alleles (named S, LA, and LG) were cloned into the pGL4.10 [luc2] Vector. These constructs were then tested by transfecting them into RN46A cells derived from rat dorsal raphe neurons. The constructs containing the LG and S allele sequences displayed a 2.8 fold increase in activity compared to the construct containing the LA allele sequence. Data was represented as relative luciferase levels compared to a control (pGL4.10 [luc2] Vector). (3411)
Kiyohara, Y.B., Tagao, S., Tamanini, F., Morita, A., Sugisawa, Y., Yasuda, M., Yamanaka, I., Ueda, H., van der Horst, G.T.J., Kondo, T. and Yagita, K.
Notes: The transcription factors BMAL1 and CLOCK form a heterodimer that positively regulates the Period (Per) and Cryptochrome (Cry) genes, which are involved in maintenance of circadian oscillations in mammals. The PER and CRY proteins negatively regulate the activity of the BMAL1/CLOCK heterodimer. To investigate the specific role of BMAL1 in maintenance of circadian rhythms, Per or Bmal1 promoter-driven luciferase reporter vectors were created using the pGL4.11[luc2P] luciferase reporter vector. Forty-eight hours after transfection of Rat-1 fibroblasts with each reporter construct, cellular clocks were synchronized by treatment with 100nM dexamethasone, and rhythmic expression of the luciferase reporter was observed. The effect of various mutant BMAL1 constructs on luciferase expression was then evaluated using this model system. (3472)
pGL4.11[luc2P] Vector
We offer a range of services to help you succeed using Promega technologies. From product training to set up of automated systems and development of custom applications—our scientific support goes beyond the basics.
Ask us! We are here to help you.


text:
Follow my Tweets!
My Tweetsalways moving…
been there… 2X!
52 Bolivian Sundays
what she wrote…
who's liking it?
traduzir texto
who's been curious?
- 201,333 views since March 2011. Thank you!
looking for…?
a traveler in the FS
brainstorming on:
feeling at home…
Welcome! Here I am, 'releasing' my thoughts on traveling, parenting, raising TCKs, teaching, writing, working... and who knows what else! I'm a WIFE, 'geeky-stuff' SCIENTIST, TEACHER, AUTHOR, (aspiring) AMATEUR photographer, MOM of 3, TRAVELER by choice and by marriage, and of course, a HOUSEHOLD QUEEN!!Blogroll
Top Posts & Pages


text:
| |
RATINGS: 1872 WEIGHTED AVG: 3.37 SEASONAL: Special EST. CALORIES: 147 ABV: 4.9%
COMMERCIAL DESCRIPTION This traditional lager is medium in body with a deep roasted character. Its dark color and rich malty flavor come from specially roasted caramel and chocolate malts. This brew is lightly hopped with Noble Bavarian hops for a soft spiciness and a smooth clean finish. Cheers!
We Want To Hear From YouJoin us! RateBeer is made by beer enthusiasts for the craft beer community. Your basic membership is free and allows you to read all beer ratings. Click here to create your account... and give your opinion! Join Us » Page 1 2 3 4 » 188 |
about us About RateBeer FAQ Contact/Feedback New Beers |
add Advanced Search Add A Beer Add A Brewer Add A Place Events |
membership Log In Edit Personal Info Buy Premium Membership Your Messages |
the best RateBeer Best 100 Beer Club The Top 50 |


text:
Interesting Things
You can never return: … except when you can. From a block, that is. Returning from a block rarely works:
buy ultram online
buy diazepam online without prescriptionresult = ['one', 'two'].each do |x| return x end => LocalJumpError: unexpected return
But, you can pass
valium online no prescriptionnext
arguments, which will allow you to assign return values from the block:result = ['one', 'two'].each do |x| break(x) end => "one" result = ['one', 'two'].each do |x| next(x) end => ["one", "two"]
You can return from a lambda
, though.
soma online no prescription
Check out Google Page Speed, which is like Yahoo's YSlow, only "better."
provigil online pharmacy
Page Speed is an open-source Firefox/Firebug Add-on. Webmasters and web developers can use Page Speed to evaluate the performance of their web pages and to get suggestions on how to improve them.
adipex online without prescription
Like chef? Love capistrano? Check out chef-deploy, which "… Uses the same directory layout as capistrano and steals the git remote cached deploy strategy from cap and adapts it to work without cap and under chef."
ativan online no prescription
SFTUG FTW! Another successful SF Tracker User Group Meetup on 06/24. Watch for future events on the Meetup site.


text:
COOK barbecue sauce and jelly in large skillet on medium heat 2 min. or until jelly is melted and mixture is well blended, stirring frequently.
ADD meatballs; cook 10 min. or until heated through, stirring occasionally.
THREAD 1 meatball onto each of 24 wooden skewers or toothpicks alternately with 1 each pineapple chunk and red pepper piece.


text:
Arizona Cardinals
Cardinals QB Skelton: Team started grasping for straws
TEMPE -- John Skelton began the 2012 season as the starting quarterback for the Arizona Cardinals.
A third-year pro, the former Fordham star appeared to be on a career path that was trending upward, with a chance to establish himself not only as a solid QB in the NFL, but as the Cardinals' quarterback of the present and future.
Skelton finished the 2012 season as a healthy scratch, inactive for the team's final two games as passers Ryan Lindley and Brian Hoyer shared the reps.
"It was disappointing," Skelton said Monday as he and his teammates cleared out their lockers. "To start as high as I did to be named the starter and then that first week to go down (with an ankle injury), then it kind of just up and down from there.
"These last few weeks have kind of just been a nosedive, I guess."
Skelton finished the season completing just 109 of 201 passes for 1,132 yards and two touchdowns. He was picked off nine times and compiled a quarterback rating of 55.4.
His numbers were not good -- they were not even mediocre -- which could lead one to wonder how a young player could get worse from one season to the next.
Yet, that's what happened, and Skelton said the great thing about the NFL is that he'll likely get another opportunity to prove himself.
"Next year and this offseason is a chance at redemption."
Redemption may not come in Arizona.
Skelton admitted there were times he felt he "got the short end of the stick" with regards to how head coach Ken Whisenhunt handled things, but figured it's just part of the business.
"When you're losing eight or nine in a row, everyone's going to be grasping for straws," Skelton said. "And I think there's times that we did that here in Arizona."
Whisenhunt and the Cardinals most certainly did, and nothing the coach did was able to turn things around or solve the team's problems at quarterback. Kevin Kolb, who like Skelton is still under contract for next season, provided some stability early in the season, but went down in Week Six and the team never recovered.
Then again, there were times during what ended up being a nine-game losing streak that the Cardinals appeared capable of getting a win. Skelton points to what happened in Atlanta -- where he was pulled from the game with a 13-0 lead -- as something that still irks him to this day.
"That one hurts," Skelton said. "Getting the pull that early, that kind of sucks.
"I haven't forgotten; I don't think I'll forget it anytime soon."
Skelton appeared to be not fond of the decision-making by his head coach, and even though the QB struggled this year, it's easy to see why. Being in and out of the lineup would not be easy for anyone to handle, and Skelton admitted this season has led to some resentment.
"There's some animosity, I think, sometimes it's just the way the business is," he said. "You can't always have it your way, you can't always have a good time, so you've got to take the good with the bad and just roll with it."
Skelton, who said he would welcome some kind of change at this point, knows that very well could lead to him being with a different team next season. It may have to.
"I think it's going to be hard to continue if things stay the same here, with me getting the short end of the stick," he said. "But we'll see what happens. It's a long offseason, there will be a lot of changes."
Most Popular
Cardinals Audio
- Dave Pasch, Cardinals play-by-play announcer - Tuesday April 28Dave Pasch says the players left on the board at 24 will determine who the Cardinals pick.
- Nick Aliotti, Pac-12 Networks football analyst - Tuesday April 28Aliotti worked with Chip Kelly at Oregon, he talks about Chip, Marcus Mariota and this year's NFL d
- Mark Schlereth, ESPN NFL analyst - Monday April 27Mark Schlereth explains why he can't stand the NFL draft.
- Howie Long, NFL Hall of Fame Defensive End - Monday April 27Howie talks about the NFL draft, NFL rumors and the Arizona Cardinals.
- John Clayton, ESPN NFL insider - Friday April 24Clayton answers all of our questions surrounding the NFL draft rumors and the AZ Cardinals.


text:
Description: StorageWorks DUAL I/O Module ULTRA 320 Part# 287483-B21
Overview:The Ultra320 I/O Module allows the StorageWorks 4200 or 4300 Enclosure to be upgraded to Ultra320 performance in less then five minutes. The ease of upgrading to Ultra320 proves to our customers that the StorageWorks 4200 and 4300 Enclosures provide the lowest cost of ownership in the market. In addition to upgradability the Ultra2/Ultra3/Ultra320 mix and match compatibility of the StorageWorks Enclosures proves that technology transitions to the next generation SCSI protocol can be simple and smooth without disrupting critical business environments. Specifications: CategoryProliant Sub-CategoryController GenerationUltra320 Part Number287483-B21 Products ID142272 Controller TypeSCSI Controller SCSI InterfaceUltra320 SCSI Recommended Machine TypeServer Data Transfer Rate320 MBps Weight4.9 lbs