text:
Nationals right-hander Zechry Zinicola has been suspended 50 games following a second violation of the Minor League Drug Prevention and Treatment Program, MLB announced this afternoon.
Zinicola's suspension is not for performance enhancing drugs but rather for a drug of abuse.
The 26-year-old right-hander is coming off a strong season in which he posted a combined 2.01 ERA, five saves and a 44-to-13 strikeout-to-walk ratio at Class AA Harrisburg and Class AAA Syracuse.
A sixth-round pick in the 2006 draft out of Arizona State, Zinicola was initially touted as a fast-track prospect who could reach the majors inRead more »
- Sec 204 Row H Seat 7 - Jan 5, 2012 at 8:41 PM
What a shame. No doubt the drug use contribute to his less than stellar performance.
- Mark'd - Jan 5, 2012 at 8:42 PM
Sad in so many respects. Hope he gets the help he needs.
- Tim the Enhancer - Jan 5, 2012 at 8:42 PM
Dumb doper. Should have stuck with martinis.
- Anonymous - Jan 5, 2012 at 8:43 PM
Bob Carpenter will return to the Mid-Atlantic Sports Network as the play-by-play voice of the Nationals, according a baseball source.Late last year, it appeared Carpenter would not return to the booth after the Nats didn't pick up his option last August, but both sides managed to come to terms recently. Calls to Carpenter were not returned.It will mark Carpenter's seventh season in the broadcast booth for MASN. He is known for the catch phrase, "See you later," whenever a Nationals player hits a home run.F.P. Santangelo will continue to be Carpenter's broadcast partner. Santangelo is the fifth TV partner for Carpenter, who has worked with Tom Paciorek, Don Sutton, Rob Dibble and Ray Knight.
- Tcostant - Jan 5, 2012 at 8:45 PM
Do they gets these guys into treatment? Last think a drug abuser needs if 50 days of free time.
- Anonymous - Jan 5, 2012 at 8:45 PM
Zig Zag is his paper of choice, no doubt.
- Grandstander - Jan 5, 2012 at 8:50 PM
Hopefully this is a wake up call and he can get the help he needs. Drug addiction is a terrible thing to go through.
- lefty1950 - Jan 5, 2012 at 8:51 PM
I guess he hangs out with Trent Williams and Fred Davis.
- Anonymous - Jan 5, 2012 at 8:53 PM
If he is suspended for the first fifty games and it begins when the season begins – does he go to spring training?
- Anonymous - Jan 5, 2012 at 8:57 PM
He is known for the catch phrase, "See you later," whenever a Nationals player hits a home run.That, or a long fly ball that approaches the fence but doesn't quite make it over. He's also known for having a sharp eye for the double play ball, which doesn't always translate into having a sharp eye for the double play. But there's one thing you can say for sure about ol' Bob. He's always one step ahead of the game.
- DC Tom - Jan 5, 2012 at 8:58 PM
Never in the history of baseball has there been a big-league player with the initials "ZZ". I suspect the pressure of this got to him.
- Anonymous - Jan 5, 2012 at 9:04 PM
Nats reportedly in the mix on Eric Chavez. LH bat who can play 3B or maybe 1B. Is Zim or LaRoche not healthy? Odd potential addition after ReRosa, no?dfh21
- bobfromalexandria - Jan 5, 2012 at 9:07 PM
sad sad sad. Now we know why he was on the fast track. He won't play in the Lerners' organization again.
- Pete - Jan 5, 2012 at 9:15 PM
Yes but ZZ Top is excellent. Also, you know who isn't suspended the first 50 games next year? Prince Fielder.
- Sec 3, My Sofa - Jan 5, 2012 at 9:16 PM
It is a shame, but I don't think we know which was first–whatever he was using, or the "unfulfilled potential" he seemed to be starting to come around on, sorta. When was the first offense? Still, too bad. capcha: "reism" — I guess the software doesn't believe in potential.
- FireAndyFeffer - Jan 5, 2012 at 9:25 PM
Three important things here:1) I feel sad for Zac and his family, maybe a trip for the Mexican Winter League (where drugs are cheap and plentiful) was not the best assignment for the Nats to send him on during the offseason.2) Horrible news that BCrap is back in the booth, watching paint dry is more exciting than listening to him and FP and don't get me started on the two radio chuckleheads, the Potomac radio guy is better than they are!3) Great news on interest in Chavez he is a good bat off the bench which is what Davey is looking for to round out the bench.
- natsfan1a - Jan 5, 2012 at 9:26 PM
That's a shame. As others noted, hope the guy gets some help to deal with it (assuming that he wants to deal with it).
- natsfan1a - Jan 5, 2012 at 9:28 PM
Also, I'm fine with Bob and FP (let the flaming commence).
- Feel Wood - Jan 5, 2012 at 9:28 PM
Nats reportedly in the mix on Eric Chavez. LH bat who can play 3B or maybe 1B. Is Zim or LaRoche not healthy? Odd potential addition after ReRosa, no?Remember when Rizzo told Boswell he was busy working on the bench, and then BINGO! he traded for Gio? Now, when all the talk is Fielder to the Nats, Rizzo is again spotted working on the bench.Working on the bench. It's Rizzo's "tell."
- Anonymous - Jan 5, 2012 at 9:29 PM
Who needs the PF Flyer when you have a FP Flyer in hand?
- Jim Webster - Jan 5, 2012 at 9:42 PM
Glad to see Carpenter back. I still have a few pages to fill in my second Carpenter score book.
- SonnyG10 - Jan 5, 2012 at 9:49 PM
I'm sad and unhappy with Zech. I hope he gets the help he needs and gets straightened out.On the Bob Carpenter note, I would be happy to see him back along with FP. In fact, I was hoping for such. They can flame me also, 1a!
- John Denver - Jan 5, 2012 at 9:55 PM
I'd love to pass the pipe with Zeckry, Trent and Fred just like I did with my ole' lady and the Danoffs back in the day.Peace,John Denver
- Young Pitchers - Jan 5, 2012 at 9:59 PM
OT-didn't see this posted yet but Darvish wants a 6/90 contract according to Japanese mediaFWIW.
- Section 222 - Jan 5, 2012 at 9:59 PM
So how come this ZZ news gets a post here, but the piece on Marlon Byrd is only on the CSN site? Seems like that one is much more discussion worthy from the peanut gallery.So I'll discuss it anyway. I'd actually love to see Marlon Byrd back in a Nats uniform. I remember admiring his hustle when he played in RFK. He might not be the best option given his salary, but I'd be delighted if he were our stopgap, and potentially a 4th outfielder once Harper comes up. Also, I think it's by no means a foregone conclusion that Harper will be ready to play full time this year. So Byrd would be a nice option if we end up going most of the year with Werth in RF and Morse in LF. Surely he's an upgrade from Bernadina.Since we're stuck with FP, I don't care much whether it's Carp or someone else doing the play by play. I'll be syncing the radio with the MASN feed as often as I can anyway. Charlie and Dave are miles ahead. At least Carp has toned down the See. You. Later. call and seemed to only do it on no doubter HRs this year. If anyone can find links to false positives, please do post. They are pretty hilarious.
- Anonymous - Jan 5, 2012 at 10:19 PM
Its ok to drink 12 beers, but don't toke on that doobie, we'll suspend you. Its a sham
- Anonymous - Jan 5, 2012 at 10:46 PM
Ok, the guy was not suspended for "drug abuse" he failed a test for the presence of some recreational drug. It's presumptive, even though this is his second offense, to call it drug abuse. Maybe it is, but maybe it is something less loaded than that.
- Mark Zuckerman - Jan 5, 2012 at 10:58 PM
Anon: This is the exact wording from MLB's press release:"The Office of the Commissioner of Baseball announced today that Washington Nationals Minor League right-handed pitcher Zechry Zinicola has received a 50-game suspension after a second violation of the Minor League Drug Prevention and Treatment Program for a drug of abuse."
- Anonymous - Jan 5, 2012 at 11:14 PM
A violation of the MLDPTP "for a drug of abuse" is not the same as being suspended "for drug abuse."
- Anonymous - Jan 5, 2012 at 11:16 PM
Zuck has it right in the body, the headline, which can be less accurate in my mind, no?dfh21
- Anonymous - Jan 5, 2012 at 11:53 PM
Anon @5:19: That's because beer is not illegal, and Augie Busch isn't shoting people in mexico and the US to control his beer turf.
- baseballswami - Jan 5, 2012 at 11:59 PM
It's his second violation? Somebody who is presumably trying to make it in the majors, knows he will be tested, and gets a second violation? This doesn't sound very recreational – this sounds like a problem. I hope he will be offered some help and he will take it.
- skidge - Jan 6, 2012 at 12:11 AM
Not sure if this has been posted here yet: http://washington.nationals.mlb.com/news/article.jsp?ymd=20120105&content_id=26272602&vkey=news_was&c_id=was. Goes to what baseballswami said.
- natsfan1a - Jan 6, 2012 at 12:16 AM
To sec222's point, I always liked Marlon Byrd, too.
- Mark'd - Jan 6, 2012 at 12:27 AM
Let's new post so we can change subjects
- Feel Wood - Jan 6, 2012 at 12:35 AM
That headline on Zuckerman's Byrd article is like when there's a 0.000001% chance of snow and the TV station fills prime time with commercials saying "Could there be snow on the horizon? Tune in at 11." If there was a way I could revoke my page view of that article, I'd do it.
- Wally - Jan 6, 2012 at 12:49 AM
The Rangers traded Chad Tracy to the Rockies.Except, I thought that we signed the guy a few weeks ago. Who did we sign?
- Anonymous - Jan 6, 2012 at 12:53 AM
What ever happened to the days when an MLB pitcher threw a no-hitter on LSD? I understand why basball needs to rigorously control performance enhancing substances, but I do not understand why players should give up their normal right to privacy to play a game. One more intrusion of self-righteous nannies into the personal lives of others. Having said this, I note that there was stupidity on the part of the player because it was not his first offense and he took a chance anyway.
- N. Cognito - Jan 6, 2012 at 1:05 AM
With the initials ZZ, could his nickname be Zig Zag?
- Buzz Killjoy - Jan 6, 2012 at 1:08 AM
A "Drug Of Abuse" could certainly be anything from sugar to heroin… the first that springs to my mind though; is alcohol-C2H5(OH)-, which is certainly the most prevalent and costly to society.
- Just wonderin' - Jan 6, 2012 at 1:38 AM
Why is the following even being posted?http://www.csnwashington.com/blog/nationals-talk/post/Fielder-to-DC-Julie-gets-the-scoop?blockID=625702&feedID=6358Which editor allowed her to use three exclamation points in a four paragraph article?
- NatinBeantown - Jan 6, 2012 at 2:06 AM
This string of comments is the absolute nadir of the offseason. 45 days never felt so far away.
- NatinBeantown - Jan 6, 2012 at 2:08 AM
…and another thing: FireAndyFeffer, you bite your tongue. Charlie and Dave are legitimately one of the best pair of radio announcers in sports. I listened to a ton of MLB radio this year, often to the other team's radio feed, and Nats fans are blessed to have them.
- Anonymous - Jan 6, 2012 at 2:16 AM
##< Yawn >##
- slidell - Jan 6, 2012 at 2:24 AM
Attaboy Beantown. (9:08) My experience too.
- Unkyd - Jan 6, 2012 at 3:59 AM
LuvLuvLuv me some Charlie and Dave!!! And I get the smallest handful of TB games, so I was scared that Dave was going to TV… For Petes sake!! You don't even need ANY commentary on TV!!
- Unkyd - Jan 6, 2012 at 4:01 AM
TV….. TV games…..
- Sec 3, My Sofa - Jan 6, 2012 at 4:29 AM
Its ok to drink 12 beers, but don't toke on that doobie, we'll suspend you.OK, looks like that changes my plans for upgrading those free beers from all those bets I'm holding. capcha: "singr"Well, after 12 beers, sure. Who isn't?
- natsfan1a - Jan 6, 2012 at 11:34 AM
You must not have been reading comments that often since the season ended, Beantown. We can sink way lower than this. ;-)NatinBeantown said… This string of comments is the absolute nadir of the offseason. 45 days never felt so far away. January 5, 2012 9:06 PM
- Buzz Killjoy - Jan 6, 2012 at 1:38 PM
"Zinicola told MLB.com that the positive test result may have come from his use of synthetic marijuana."I got done playing winter ball and I decided to have a little fun," he said. "I tried out this synthetic marijuana. I tried it a few times … and I got drug tested. It's pretty much what it is. I take responsibility for it. There is no excuse." ""synthetic marijuana" the "Bathtub Gin" of a new generation under a new prohibition regime.Chances are it has roughly the same affect as the real thing, hours of effective intoxication yet 30 days of persistence in the body for "testing" purposes.
- NatsNut - Jan 6, 2012 at 2:15 PM
It's kinda sad how relieved I was that it wasn't for PEDs.


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University of California, Berkeley | |||||
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Jepson eFlora: Taxon page
Key to families | Table of families and genera |
Indexes to all accepted names and synonyms: | A | B | C | D | E | F | G | H | I | J | K | L | M | N | O | P | Q | R | S | T | U | V | W | X | Y | Z | |
Annual to perennial herb (to tree). Leaf: cauline or basal, alternate, opposite, or whorled, generally simple and toothed (to pinnately compound); stipules 0 or generally deciduous. Inflorescence: spike, raceme, panicle, or flowers 1 in axils; bracted. Flower: generally bisexual, generally radial, often opening at either dawn or dusk; hypanthium generally prolonged beyond ovary (measured from ovary tip to sepal base); sepals 4(2–7); petals 4(2–7, rarely 0), often fading darker; stamens 2 × or = sepals in number, anthers 2-chambered, opening lengthwise, pollen interconnected by threads; ovary inferior, chambers generally as many as sepals (sometimes becoming 1), placentas axile or parietal, ovules 1–many per chamber, style 1, stigma 4-lobed (or lobes as many as sepals), club-shaped, spheric, or hemispheric. Fruit: capsule, loculicidal (sometimes berry or indehiscent and nut-like). Seed: sometimes winged or hair-tufted.
22 genera, ± 657 species: worldwide, especially western North America; many cultivated (Clarkia, Epilobium, Fuchsia, Oenothera). [Wagner et al. 2007 Syst Bot Monogr 83:1–240] Gaura moved to Oenothera. Fuchsia magellanica Lam. naturalized in northern California. —Scientific Editors: Robert Patterson, Bruce G. Baldwin.
Unabridged references: [Munz 1965 North America Fl II 5:1–278]
Annual < 1.5 m. Stem: prostrate to erect, glabrous, often glaucous, or puberulent (hairs long, spreading). Leaf: pinnately veined; petiole < 4 cm or 0; blade 1–10 cm, linear to elliptic or ovate, entire or shallow-toothed, glabrous or sparsely puberulent. Inflorescence: spike, raceme; bracts leaf-like; axis in bud straight or recurved at tip, in flower ± straight; buds erect or not. Flower: hypanthium obconic to cup-shaped, or long, slender, generally with ring of hairs within; sepals 4, generally fused to tip in bud, reflexed at least at base, staying fused at least at tip, in 4s or 2s, or all coming free; corolla bowl-shaped to rotate, petals 5–60 mm, often lobed or clawed, lavender or pink to dark red, pale yellow, or white, often spotted, flecked, or streaked with red, purple, or white; stamens 8, in 2 like or unlike series, or 4, filaments cylindric to wider distally, subtended by ciliate scales or generally not, anthers attached at base, pollen white or yellow to blue-gray, lavender, or ± red; ovary 4-chambered, glabrous or not, cylindric, fusiform, or wider distally, generally shallowly to deeply 4- or 8-grooved, stigma lobes 4, generally prominent. Fruit: generally capsule, elongate (short, indehiscent, nut-like). Seed: generally many, rarely 1–2, 0.5–2 mm, angled, crested or not, brown, gray, or mottled.Key to Clarkia
± 41 species: western North America, 1 South America. (Captain William Clark, 1770–1838, of Lewis & Clark Expedition) [Lewis & Lewis 1955 Univ Calif Publ Bot 20:241–392] Self-fertile; self-pollinated or outcrossed; on herbarium specimens, curvature of inflorescence axis in bud generally reliable, pollen color generally not.
Stem: decumbent to erect, < 1 m, puberulent. Leaf: petiole < 1 cm; blade 1–6 cm, linear to lanceolate. Inflorescence: open or dense; axis in bud straight; buds erect. Flower: hypanthium 3–10 mm; sepals staying fused in 4s; corolla bowl-shaped, petals obovate to fan-shaped, pink to lavender, rarely white, red spot or marks near middle; stamens 8, anthers alike; stigma exserted beyond anthers.
n=7. Intermediates among subspecies frequent. Other subspecies to British Columbia. [Online Interchange]
Stem: decumbent. Inflorescence: dense; internode generally < subtending flower. Flower: petals 20–35 mm; ovary cylindric, grooves 4.
Open slopes, bluffs on or near coast; < 100 m. North Coast, n Central Coast. Jun–Aug [Online Interchange]
Previous taxon: Clarkia amoena
Next taxon: Clarkia amoena subsp. huntiana
Citation for the whole project: Jepson Flora Project (eds.) 2013. Jepson eFlora, http://ucjeps.berkeley.edu/IJM.html, accessed on May 6 2015
Citation for this treatment: [Author of taxon treatment] 2013. Clarkia, in Jepson Flora Project (eds.) Jepson eFlora, http://ucjeps.berkeley.edu/cgi-bin/get_IJM.pl?tid=49885, accessed on May 6 2015
Copyright © 2014 Regents of the University of California
We encourage links to these pages, but the content may not be downloaded for reposting, repackaging, redistributing, or sale in any form, without written permission from The Jepson Herbarium.
Clarkia amoena subsp. amoena See CalPhotos for additional images © 1995 Saint Mary's College of California |
Geographic subdivisions indicated for the distribution of Clarkia amoena subsp. amoena | Markers link to CCH specimen records. If the markers are obscured, reload the page [or change window size and reload]. Yellow markers indicate records that may provide evidence for eFlora range revision or may have georeferencing or identification issues.
(Note: any qualifiers in the taxon distribution description, such as 'northern', 'southern', 'adjacent' etc., are not reflected in the map above, and in some cases indication of a taxon in a subdivision is based on a single collection or author-verified occurence).
View elevation by latitude chart |
Data provided by the participants of the Consortium of California Herbaria.
View all CCH records
CCH collections by month |

subset: commoncrawl


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Out of Stock
Item # CST0670
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Castelli Iride Seamless Short Sleeve Top $0.00
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Scot B.
Nice base layer!
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This would be ok compression but I have a torn rotator cuff. Fit and finish and general workmanship is very good.
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Nice base layer. Not sure it cools or warms you any better than a Castelli similar product at half the price. Very comfortable and form fitting, which is appreciated.


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While your kid may not be a certified city planner, this block set will at least provide them with the proper training. It's colorfully dyed in water-based inks then hand-sanded for a beautiful look.


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Purchased, and opened a bottle of EC 12 last night as it showed up a lot in the previous four pages of posts.
EXACTLY what I was looking for from you guys! Got a list of another 8 or so to try.
Eight's a good start.
Good luck with that. I had the same plan but now I only purchase on special occasions......... and when the sun is up.
They say some of my stars drink whiskey, but I have found that ones who drink milkshakes don't win many ball games-Casey Stengel
im with you on that.....one a month. something new each time, until i find things that im determined to keep around. then it stays in the shelf stuff as i continue the one a month search. right now i only have two that stay on the shelf......KC120 and OGD 114. once i polish off the remains of the VOB BIB and OGD BIB, then i'll bring in a new one. im thinking of bringing an Ezra in next.........now, should it be the 7/101 or the 12/99?
decisions, decisions....
One case, yes, that's it, just the one.
WTKS (dark top if I can find it)


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Inaugural Rush Select ID camp underway in Casa Grande.
Last Updated on Friday, 07 December 2012 09:57
The purpose of the camp is to help identify and train the players to make the traveling squad for the annual Rush Select event at the Presidents Day Tournament in Phoenix on February 2013. The first training session started at 4:00pm on Friday and the boys were back out there at 7:30pm to play the first 11v11 games under the lights. The Select Staff are able to have all their needs taken care of at the Casa Grande resort, with 8 immaculate fields (6 lighted), hotel and banquet facilities as well as classroom, locker room and meeting rooms.
Saturday saw the boys back out after breakfast for a morning training session, which was followed by watching the LA Galaxy vs. Real Salt lake U18 Academy game before heading in to lunch. The boys had the pleasure of speaking with two former Rush players that are now part of the residency program for Real Salt Lake here at Casa Grande, and hearing about their experience and what it takes to become an Academy Player. The afternoon saw a second training session of the day, and the evening back to 11v11 under the lights and a second classroom session.
Players coming from 18 Rush Clubs are represented in Arizona, all looking to join the event in February. Sunday will see the final day of training and games before they all head back to their home states. For updated information please go to the Facebook/rushselect for photos and updates.


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Click through for our hate-'em-Tatum shots!


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Top Definition
A tool or technique that makes some aspect of one's life easier or more efficient.
Web sites now exist, where lifehacker (followers of the movement) can trade lifehacks - suggestions on how to reduce chaos and make their lives more enjoyable.
Beküldő: JRob
2005. december 6.
4 more definitions
Together the words "life" and "hack" create a phrase that describe any hacks, tips and tricks that get things done quickly by automating, increase productivity and organizing.
I was the most disorganized person ever. A few life hacks later I get more done in a day than I used to get done in a week.
Beküldő: good karma
2006. január 15.
A term the technologically (mostly) illiterate throw around to demonstrate that they understand these newfangled computers. Formerly known as "good ideas."
A: "Did you know that if you take a shampoo bottle, pour some shampoo on your hand, and mush it into your head when you're in the shower, your hair will smell better? Lol, my favorite life hack!"
B: "Shut up."
B: "Shut up."
Beküldő: delroah
2013. november 1.
A science-fiction novel about nanomachines being used to create a zombie outbreak.
The protagonist is a lesbian who falls for a girl who is straight.
Not focused heavily on horror, Lifehack is heavily character driven.
The protagonist is a lesbian who falls for a girl who is straight.
Not focused heavily on horror, Lifehack is heavily character driven.
Lifehack is written by Joseph Picard, and can be found on Amazon, smashwords, ozero.ca, and probably a bunch of other sites.
Beküldő: Ozero
2011. november 2.
Someone who does not know how to live well. A person who never gets the big picture.Someone who dwells on unimportant things.
I was talking to fran about the big questions of life, and all the sudden dick walks up and pops off with,"so are we gunna order some pizza or what?" What a lifehack!
Beküldő: bezel333
2005. december 31.


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Powerful Delivery: Greater Efficiency
Efficient Transfection of Primary Neuronal Cells with TransFectin™ Lipid Reagent TransFectin lipid reagent demonstrates gentle yet effective transfection of primary rat hippocampal neurons.
Efficient tranfections of primary neuronal cells with TransFectin lipid reagent. Primary rat hippocampal neurons were transfected on coverslips in 24-well plates using 1 µg of a GFP expression plasmid and 2 µl TransFectin. At 3 hr after complex addtion, the medium was replaced with 50:50 conditioned:fresh medium. Neurons were fixed and analyzed 48 hr after transfection. Data courtesy of Dr. Janil Kanaani, Diabetes Center, University of California, San Francisco.
Protocol for Transfection of Rat Hippocampal Neurons with TransFectin Lipid Reagent
Constructs used for transfection are: (1) pEGFP-N3 (4.7 kb) from CLONTECH; (2) fusion protein of Glutamic acid decarboxylase 65 and GFP (GAD65-GFP; 6.4 kb). Data courtesy of Dr Jamil Kanaani, Diabetes Center, University of California, San Francisco.
Effective Delivery into CHO and NIH3T3 Cells Using TransFectin Lipid Reagent TransFectin lipid reagent demonstrates high transfection efficiency of CHO and NIH3T3 cells.
CHO and NIH3T3 cultures were transfected with pCMV.SPORT-β-gal using Bio-Rad's TransFectin lipid reagent. Cells were stained with X-gal to determine β-galactosidase activity 24 hr posttransfection. Blue cells indicate successful transfection.
Lower Cytotoxicity: Increased ViabilityTransFectin™ lipid reagent provides exceptional performance with less cytotoxicity than is typically observed in other high-efficiency transfection reagents. Very high efficiencies may be obtained while maintaining the viability of your cells. The combination of high efficiency and lower cytotoxicity results in greater levels of reporter gene expression.
Total protein assays provide an accurate assessment of culture health after transfection. Bradford assays performed on cell cultures 24 hr posttransfection indicate that TransFectin-treated cultures are typically healthier than cultures exposed to other lipid reagents.
Cytotoxicity comparisons based on total protein assays. A, COS-7 and B, HeLa cells were transfected at 90% confluence using TransFectin (orange) and another cationic lipid reagent (blue) in a single 24-well culture vessel. Total protein was determined using the Bradford reagent 24 hr posttransfection. Higher OD values correlate with more protein, allowing an assessment of the relative toxicity of the lipids.
TransFectin™ Lipid Reagent Outperforms in Transfection Efficiency
TransFectin lipid reagent illustrates high transfection efficiency in a broad range of commonly used mammalian cell lines, as it outperforms competing lipids in the data shown. For some cell lines, transfection efficiencies >90% are routinely achieved using TransFectin. In addition, use less reagent per experiment than is needed for many other products, while still achieving excellent performance.
Relative activity in three cell lines. Cells were treated with pCMV.SPORT-β-gal using TransFectin lipid reagent (orange) and three other lipid reagents. Cell cultures were assayed using ONPG 24 hr posttransfection and OD readings were taken to quantitate β-galactosidase expression. The cells transfected with TransFectin clearly illustrate higher transgene activity, thus indicating greater expression and transfection efficiency.
Transfection efficiency comparisons. A variety of cell lines were transfected in 24-well plates with pCMV.SPORT-β-gal using Bio-Rad's TransFectin (orange) or another popular lipid transfection reagent (blue). At 24 hr posttransfection, cells were lysed and stained with X-gal. Efficiency is reported as the percentage of cells transfected (expressing β-galactosidase) in the population.
Effective delivery into CHO cells using TransFectin lipid reagent. A, B, C, CHO cultures after transfection using three commercially available lipid transfection reagents and D, TransFectin. Cells were stained with X-gal to determine β-galactosidase activity 24 hr posttransfection. Blue cells indicate successful transfection.
High Performance at Multiple Confluences The TransFectin™ lipid reagent protocol allows you to achieve high efficiencies across a wide range of culture confluences, while maintaining a high level of consistency and performance. Perform transfections with or without serum-containing medium at a culture confluences between 40% and 90%.
COS-7 (orange) and HeLa (blue) cells were transfected at confluence between 40% and 90% with pCMV.SPORT-β-gal. Cell cultures were assayed using ONPG 24 hr posttransfection to quantitate expression.
Consistency Using a Variety of Vessel Sizes Achieve consistent levels of expression with a variety of cell culture vessels. The graph below shows the amount of expression achieved using different-sized culture vessels.
A comparison of β-galactosidase expression in different-sized culture vessels. HeLa cells were transfected with pCMV.SPORT-β-gal at 70% confluences, in the presence of serum, using TransFectin lipid reagent. Cells were assayed for β-galactosidase activity using ONPG 24 hr posttransfection to quantitate expression. Results show that reporter gene activity is scalable with vessel size when using TransFectin lipid reagent.
Suggested Reagent Quantities for Different Sizes of Plates/Wells
The table below provides approximate reagent requirements for the most popular vessels. For unlisted vessels, simply determine the change in surface area and linearly scale the reagent volume.
Effective Delivery into Suspension Cells
TransFectin™ lipid reagent illustrates efficient delivery into K562 (suspension) cells.
Efficient delivery. K562 cells were plated in 24-well plates at a density of 4 x 105 cells/well. TransFectin: plasmid DNA complexes were formed by combining 1.25 µg (dark orange) or 1.00 ug (light orange) pCMV.SPORT-β-gal with TransFectin lipid reagent. After 20 min, transfection complexes were added to the cells in the presence of serum. At ~24 hr posttransfection, cells were pelleted and lysed, then assayed for β-gal expression.
Protocol for Transfection of Suspension Cells (24-Well Plates)
Protocol for Transfection of Adherent Cells(24-Well Plates)
Optimization Determining the optimum conditions for transfection efficiency is essential for maximizing gene expression and minimizing cellular toxicity. The two most important parameters to optimize transfection efficiency using TransFectin are: amount of TransFectin and concentration of nucleic acid. In general, gene expression will increase, plateau, and then decrease with increasing volumes of TransFectin. This decrease in gene expression correlates with reduced cell viability. As increasing amounts of plasmid are added to the cells, gene expression will increase, then plateau, or even decrease since the amount of DNA used can directly affect toxicity. If toxicity is encountered, try reducing lipid or DNA amounts used in the transfection. The concentration of TransFectin and the amount of plasmid required for maximal expression may vary from one cell line to another. The highest levels of expression can be obtained using amounts of TransFectin and DNA suggested in the table below for given culture vessel sizes.
Other Recommendations for Best Results
Additional Optimization Resources
Optimization of TransFectin Lipid Reagent-Mediated Transfection for Different Cell Types
It is important to optimize transfection conditions not only for each transfection method but also for every cell type. Cell density, duration of transfection, volume of medium during transfection, and ratio of lipid reagent to DNA are key factors for efficiency. In this article we describe the optimization of transfection with TransFectin monitored by a secreted alkaline phosphatase (SEAP) reporter gene assay. We compare two suspension cell types that are considered difficult to transect: bovine peripheral blood mononuclear cells (PBMCs) and bovine B-lymposarcoma BL3.1 cells (Anderson et al. 2004, Fenton et al. 1998). Additionally, we optimize the transfection efficiency for two adherent cell types: primary bovine oviductal cells and a calf pulmonary artery endothelial cell line (CPAE).
The Transfection Protocol Online Library contains protocols obtained from the literature, developed by Bio-Rad scientists, or submitted by scientists like you. browse protocols to view our library and find your starting point.
Bio-Rad Laboratories has helped scientists deliver molecules into cells for over 35 years. Instruments, including the Gene Pulser® series of electroporators, the MicroPulser™ electroporator, and the Helios® gene gun and PDS-1000/He™ biolistic systems, have been used successfully to transfect prokaryotic and eukaryotic cells, including plant and animal cells. These transfection instruments are cited extensively in the literature. Our chemically mediated methods include lipid-based transfection reagents — TransFectin™ lipid reagent for delivery of plasmid DNA and siLentFect™ lipid reagent for siRNA delivery.
TransFectin reagent is a cationic lipid reagent that delivers the highest efficiencies and expression levels over a broad range of cell lines, including some difficult-to-transfect and primary cells, while minimizing damaging cytotoxic effects observed with other high-efficiency general transfection reagents. It provides high transfection efficiencies with lower amounts of lipid than most other lipid reagents, allowing more experiments per vial of reagent and lower costs per transfection.
TransFectin lipid reagent is a mixture of a proprietary cationic compound and a co-lipid DOPE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine). These compounds have been optimized for intracellular delivery of nucleic acids into cultured mammalian cells in the presence of serum at cell densities from 40 to >90%. For most cell lines, high levels of expression can be obtained using concentrations of TransFectin reagent and DNA suggested for given sizes of plates/wells.
Key Features
Other Lipid Reagents
Bio-Rad offers a family of lipid transfection reagents, each of which is optimal for certain applications. TransFectin lipid reagent is a general-purpose lipid that is ideal for plasmid delivery. siLentFect™ lipid reagent is an RNAi-specific lipid that is ideal for siRNA delivery. It can also be used to perform cotransfection with a small reporter-plasmid along with the siRNA molecule.
What is the education discount policy?
For more than 15 years, Bio-Rad has made science education a major priority. To support this effort, the company has implemented a discount policy that allows high school and college teaching laboratories to purchase kits, instruments, reagents, and other equipment at preferred prices.
How do I apply for the education discount?
If you are an educator at the high school or college level, visit our Education Discount Policy page to establish an education account number.
170-3350 0.5 ml, lipid transfection reagent, for a variety of applications including plasmid DNA, siRNA, and shRNA delivery
170-3351 1.0 ml, lipid transfection reagent, for a variety of applications including plasmid DNA, siRNA, and shRNA delivery
170-3352 5 x 1.0 ml, lipid transfection reagent, for a variety of applications including plasmid DNA, siRNA, and shRNA delivery
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Have You Overlooked the Obvious? 12 Ways to Win Major Value with SharePoint Apps
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From the Archive
Ariel Sharon: 1928 - 2014
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January 11, 2014
by Magnum Photographers
Israeli general and statesman Ariel Sharon has passed away at the age of 85.
Considered one of Israel's greatest generals, Sharon joined the Haganah in 1948, fought in the War of Independence and then served as a commander during the 1956 Suez War, Six Day War, and Yom Kippur War.
As Minister of Defense, Sharon oversaw military operations during the 1982 Lebanon War. In September of that year, Christian Phalange militia entered the Sabra and Shatil refugee camps in Beirut and massacred between 800 and 3500 Palestinian civilians while Israeli forces surrounded both. Though his forces were only indirectly involved, Sharon was considered responsible for allowing the killings to occur and resigned in 1983.
Soon after, he returned to politics and served as Minister of Industry, Trade and Labour, Minister of Housing and Construction, Minister of Energy and Water Resources, and Minister of Foreign Affairs.
Sharon was elected Prime Minister in 2001. In 2006, while still in office, he suffered a stroke which forced him to spend his final years in a vegetative state.


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Four additional cases of Hantavirus Pulmonary Syndrome (HPS) stemming from infections that occurred after staying in cabins in Yosemite National Park were reported today, Friday, Aug. 31, according to the California Department of Public Health (CDPH).
Two others have now died of the Hantavirus, three have recovered and one is hospitalized but improving, according to park officials. This brings the total number of people infected with the hantavirus who visited Yosemite National Park to six.

A totally random sampling of what Mammothites are reading, culled from here and there, appearing every now and then.
The June Lake community is alive with ambitious plans for this winter, despite a brief tussle between Mono County Supervisors over how to monitor and allocate the $100,000 the supervisors gave the community as "bridge" money.
At the top of the list are expanded cross-country ski and snowshoe trails, a snow play area, a Holiday Village atmosphere, an ice-skating area and, even, dog sled rides.
Mono County is still in relatively good financial shape compared to most counties in California, but its emergency reserve fund is down sharply since 2008.
On top of that, current anemic property tax revenues mean tight times ahead.
"We are in pretty good shape, but since the housing crisis hit in 2008, we have cut the reserve from $5.8 million to its current number of $1.6 million and I believe it's time to begin rebuilding," said Brian Muir, the county's finance director.
"It's all right that we have done this; that's what the reserve is for. But it is time to rebuild."
Travelers and visitors headed into the Sierra Nevada for the Labor Day weekend are invited to enjoy free downloads of a new mobile phone app for the National Geographic Sierra MapGuide by searching for the "Sierra MapGuide" on your iPhone or Android mobile device.
Sierra Nevada travel tips, restaurants, tourist information and more are now as close as your smartphone thanks to these new apps available for iPhone and Droid devices.
Fire danger in the Eastern Sierra has eased somewhat following an unusually wet August, but that doesn't mean it is time to relax.
According to fire officials, September can be one of the warmest and driest months. If that pattern holds, it could erase much of the gains made in August, when almost three weeks of heavy rain took the sharp edge off the drought.
The Bristlecone Pine Forest Visitor Center celebrates its grand opening tomorrow, following a fire that completely destroyed the popular visitor center several years ago.
The grand opening celebration is sure to be an enjoyable event for visitors and locals alike who have donated to and supported the reconstruction effort, and for those who have been waiting in anticipation for the opening of the new facility.
The celebration will include guest speakers, music, an art exhibit, and a ribbon cutting for the new visitor center.
We'd like to think we live in a fishbowl here—that everyone in California (and beyond) is paying attention to us.
They're not.
We are a small town in one of the most remote, inaccessible areas this side of the Pakistan/Afghan border.
We all signed up for that, for a variety of reasons. But for those who may wish for thoughtful attention from Sacramento or L.A., not to mention Washington, D.C., it is best to get used to how small our fishbowl really is.
The following locations have been stocked with Alpers Trophy Trout from Conway Ranch in time for the Labor Day weekend, beginning with the areas near Bishop and moving north toward Bridgeport.
• Intake II • South Lake • Sabrina • Bishop Creek • Tinnemaha Creek • Taboose Creek • Independence Creek • Lower Twin Bridgeport • Upper Twin Bridgeport • Bridgeport Reservoir • Convict Lake • Grant Lake • Gull Lake • June Lake • Silver Lake • Lundy Lake • Mammoth Lakes Basin—Mary, Mamie, Georg and Twin • Rock Creek • Rock Creek Lake • Little Virginia
One way to cut county expenses is to cut fuel use by the county by 10 percent, says Mono County Supervisor Larry Johnston. He pushed the idea during this week's county budget hearings, hoping to gain some traction.
Although the supervisors did not immediately endorse Johnston's idea, they said the idea merited further discussion.
Mammoth Unified School District is soliciting letters of interest from interested community members and staff to service on the district's Measure S Citizens' Oversight Committee. The purpose of this committee will be to inform the community concerning the expenditure of the Measure S parcel tax revenue. Anyone interested should direct a letter of interest to the district office by the close of business today (Friday).
"What a cute boy!" said the stranger.
"Who, me?!?!?" Fido asked, blushing.
The stranger walked over to Fido, who was blissfully sitting under a table at last weekend's "Mammoth Rocks/A Taste of the Sierra" festival in the Village. He was manning the booth for the paper.
"Are you the dog in the paper all the time?" she said.
"That's me!" Fido said, and he wagged his stump.
"Want to hear a story?" Fido said to the woman.
"Why, yes, Fido," she responded. "I love stories."
Fido wormed his way from beneath the table—not an easy thing to do for a big, red, 80-pound chow mix.
Mammoth's softball teams finish their seasons this week with dizzying rounds of multiple playoffs.
Monday, Aug. 27, was the start of the single-elimination playoffs, with Greyhawk, Originales, Grumpy's Holy Rollers, Austria Hof-Thunderstruck, Guerreros and Los Mayas in the hunt.
As a reward for finishing first in the regular season, XRC won a first-round bye.
On Tuesday and Thursday, the two Co-ed Divisions (14 teams total) began tournament play, and to try to name a favorite would be futile.
The shovel goes into the ground on Tuesday for the long awaited, much anticipated Gateway Monument signs on S.R. 203 coming in and out of town. Elizabeth Tenney, Jim Tenney, Bruce Woodward and et al showed up at the Town Council meeting Wednesday to make sure it was really going to happen. Critics of the thing stayed home in droves, save one. …
For the first time in 12 years, high school football has disappeared from the airwaves.
KSRW (Sierra Wave) sports director Bob Todd said the station dropped the broadcasts "for a number of reasons," but declined to go into specifics.
The decision is a blow to high school sports fans, as well as to the teams themselves, said Huskies Head Coach Marty Thompson.
"It's a huge deal to those people who watch the games, and it was a great tool for us."
It also was a public service that not many communities have ever had a chance to experience.


text:
Top Definition
1) The act of kicking ass.
2) Something everyone should fear.
2) Something everyone should fear.
1) I am experienced in the practice of martial arts, which is not good for anyone reading this example.
2) Again I practice martial arts.
2) Again I practice martial arts.
av Garit The All Mighty
3. februar 2009
1 more definition
Another way of saying that something isn't THAT difficult, keeping in mind that martial arts aren't that easy. Used in a simliar manner as rocket science (when used sarcastically.)
I asked my mom to scan my essay and email it to me, but she found it too complex. I tell her it isn't a martial art, so she hangs up on me and I got an F for that essay.
av umbepo
14. november 2005


text: A longtime landmark in the Indian Beach neighborhood, Jungle Gardens has enthralled generations of visitors with its wild creatures and exotic foliage. But artist John Pirman, who recently moved to the nearby Sapphire Shores neighborhood, says he finds the entrance pavilion as intriguing as what's inside. The futuristic 1960s-style architecture, with those "multiple witches'- hat roof lines tells visitors they are about to experience a very special place," he says.


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Teaching Toolboxes
Reservations for the 2014 - 2015 school year are now open!
Teaching Toolboxes are teaching resource kits, each complete with touchable specimens, reproducible materials, and related books and information. Designed for K-12 students and community groups, our toolboxes not only allow for unique teaching opportunities, but provide subjects that excite students about the natural sciences. Our museum offers a wide variety of topics, which include:
Bats | Bison | Bones |
C.I. Water | Digging Dinosaurs | Field Tools |
Insects | Nanotechnology | Native Utah |
Soils | Traits | Utah's Fossil Past |
Parasites | Plants | (English and Spanish text included) |
*These boxes are available to check out for free and can be reserved for a two-week period. Information about the individual contents of the toolboxes can be found at the bottom of this page.
Submit a Request for a Teaching Toolbox Here
Please note the following toolboxes are unavailable until the 2015-2016 school year :
Utah's Fossil Past
Native Utah
Reservations for the 2015-2016 school year will open May 11, 2015.
Not near the Museum? Reserve a Toolbox from your District Office instead! Click Here for a Listing of Off-Site Locations!
Questions? Please email us or call: 801-585-5237
Please Note:
Teaching Toolbox Reservation Process
- Submit your Teaching Toolbox Request at the link above.
- You will receive an email confirmation of your request from the Museum.
- Once confirmed, you will pick-up your Teaching Toolbox during your designated reservation period at the Rio Tinto Center. Directions to the Rio Tinto Center.
- Toolboxes are available for pick-up and drop-off Saturday - Tuesday from 9:00 am until 5:00 pm.


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Calendar Of Events
Breastfeeding Support Group
Date: Tuesday, October 15, 2013
Time: 10am to 11am
Location: South Clinical Campus
Board Room / 1 [Map]
Room: Board Room (Floor: 1)
Contact name: Bonnie Gagnon
Contact phone: 262-8447
Contact email: email@example.com
Mothers and their babies are invited to attend Albany Medical Center's Breastfeeding Support Group. This group provides breastfeeding mothers the opportunity to meet other moms for support, information and encouragement in a nurturing environment. An Albany Medical Center Lactation Consultant will lead the group. Registration is NOT required, please attend on a walk-in basis. Free parking is available in front of the South Clinical Campus, 25 Hackett Blvd., Albany, NY. PLEASE NOTE: We will not meet if there is inclement weather, school closings or delays. |


text:
Top Definition
A buffoon. An unskilled person. From the McDonald's practice of awarding stars on employees' badges denoting skill or experience level. Thus an employee with no stars is unskilled, which is a joke considering they work at goddam McDonalds. I mean, how hard can it be???
Don't listen to him, he's a Jimmy-no stars!
Gezza T가 작성
2003년 04월 20일 (일)


text:
3rd Generation Intel® Core™ i5-3317U Processor
Ultra responsive. Ultra sleek. Ultrabook™.
Intel® Core™ i5-3317U Processor
The Intel-inspired Ultrabook™ combines power and design, delivering an optimal blend of responsive performance and stunning visuals in the portability and style you want.1 Powered by the Visibly Smart Intel® Core™ processor, the new Ultrabook™ enables smart multitasking for creating, exploring, and enjoying what interests you most.2 Experience the joy of style and performance. Because the only thing more amazing than Intel® technology is what you'll do with it.
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Intel® Core™ i5 Processors Notebook PC Configuration Details
Toggle Open/ClosedIntel® Processor Name |
Intel® Core™2 Duo Processor P8600 |
3rd Generation Intel® Core™ i5-3317U Processor |
Number of |
2/2 |
2/4 |
Processor speed |
2.40 GHz |
Up to 2.60 GHz |
Processor cache |
Unified 3MB L3 |
Platform manufacturer |
OEM system (modified) |
OEM system (modified) |
Chipset |
Mobile Intel® GM45 Express Chipset |
Mobile Intel® UM77 Express Chipset |
Chipset Intel® INF file |
184.108.40.2060 |
126.96.36.1990 |
Platform BIOS |
System memory |
4GB (2x2GB) Micron* DDR3-1066 7-7-7-20 1T |
4GB (2x2GB) Samsung* DDR3-1600 11-11-11-28 1T |
Hard disk |
Hitachi* HTS725032A9A364 320GB 7200RPM |
Hard disk driver |
Intel® Rapid Storage Technology (Intel® RST) Intel® 184.108.40.2066 |
Intel® Mobile Express Chipset SATA RAID Controller Intel® 188.8.131.522 |
Video card |
Intel® GMA 4500 |
Intel® HD4000 Graphics |
Video driver |
184.108.40.2065 |
22.214.171.12461 |
Graphics resolution |
1366 X 768 resolution, 32-bit color |
1366 X 768 resolution, 32-bit color |
Operating system |
Microsoft Windows 7* Ultimate 64 bit SP1, on NTFS |
Microsoft Windows 7* Ultimate 64 bit SP1, on NTFS |
Microsoft DirectX* version |
Microsoft DirectX* 9 |
Microsoft DirectX* 11 |
Power management mode |
High performance |
High performance |
Additional information: 5


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Course Details Course Description About The Instructor |
This course satisfies part of the Communication Skills graduation requirement.
This section is Communication Skills intensive. Communication Skills I-V must be completed before taking this course. Session, Dates: 3 (01/13/2014 - 03/07/2014) Days: R Time: 06:00 - 10:00 pm Location: Moon Campus Room: Hale Center 304 Seats Available: 5 Seats Credits: 3 |
INFS4810 Project Management, the capstone course, utilizes an Information Systems Project Management Taxonomy to provide the student with an integrated theoretical and practical perspective of technology and information systems as process and functional components of an organization. Emphasis is on the roles that people, information, information systems quality, and technology play in the application of the nine Knowledge Areas and five Process Groups that comprise the Common Body of Knowledge for Information Systems Project Management. Particular emphasis is placed in the five Process Groups?initiating, planning, executing, controlling, and closing--- for information systems projects that are needed to provide the various types information systems which provide a competitive advantage in both inter-organizational and international environments and yet serve the needs of both the users and management. As an underlying tenet for the nine Functional Areas, the course emphasizes refining individual communications skills necessary to the field of information systems project planning and management. The student completes Chapter Questions and case studies, practices team problem solving techniques, participates in structured group discussions, conducts research, writes reports, and gives oral presentations.
Lawrence C. Franzi, D.Sci.
Part-Time Faculty
Computer and Information Systems
email@example.com Wheatley Center 142 Profile |


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User Tag List
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- Join Date
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Sindhu hasn't been performing that well in the youth circuit recently, but in my opinion she's the 2nd or 3rd best U19 in the world at the moment (the only one who's definitely better is Ratchanok Inthanon); and you're right, I think she needs to work on stamina, like all Indian players. But her natural tools are brilliant in general; she has all the potential it takes to become a World Champion.
However, having seen them both, I would firmly say that Rituparna Das (1 year younger) is even better. She might be about the same currently, but every year she's improving faster than Sindhu. I'd keep a closer eye on her; I think she's already better than Sindhu.
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let see
in 2010
she lost to nazomi,geng jian,suo di
in 2011
she lost to sun yu and sonia cheah
with ratchanok and tai at the top(with significant world ranking)
china ws who is always strong(3 out of 4 slot in ajc semi final recently)
i would think twice to rank her in U 19,probably 5-8 but definitely not in top 4 i would say
Rituparna Das,any video?
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If Rituparna Das is already better than Pusarla Venkata Sindhu (according to GameGod's Post #53), then Rituparna Das (not Sindhu) would be the one for us to watch.
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Did she really lose that badly in 2010? I don't know where all these results have come from. Where do you find them? In any case, the other players are much older than her (except for Ratchanok) and she is improving reasonably quickly. That said, clearly on this topic you are more knowledgable than me (my only experience is from having watched Sindhu play live quite a few times, but I have also seen Ratchanok).
I don't yet have any videos of Rituparna Das (I couldn't find one on YouTube); in any case, if you wouldn't rate Sindhu in the top 4 in the world you almost certainly won't rate Rituparna Das either, as their levels are pretty similar for my money (IMO Rituparna Das might have a very slight edge, but not a big one).
In my opinion Rituparna Das is better than P. V. Sindhu, but then I've seen Rituparna play very many times and my decision is based purely on her superior speed and slightly greater agility around the court. But at this stage I don't have any international results to back me up yet, because (to my knowledge) Rituparna has not yet played a major international Youth tournament. She's also very inconsistent, she has lost to P. V. Sindhu three times (I watched one of those matches), although she has also won quite a few times. This might be why Gopichand doesn't let her play internationally yet. If you know of any International tournaments she's played in, I would be happy to hear about it. That said, of course I stick by my original statement that Rituparna Das is the one who will eventually be the best.
BTW, has anyone heard of my results from Rohit Yadav (U19 BS)? He's third on the current national rankings, but I don't know of any International results he's got in the last few months.
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asia junior championship 2010 team event,lost to nazomi
asia junior championship 2010 individual event,2nd round,lost to geng jian
world junior championship 2010 individual event,lost to suo di
cant recall who is R Das,so probably she havent come out and play yet
i can only tell if i saw her games
An interesting interview
Here is an interesting interview with Sindhu as well as Gopi Chand.
VOL.34 :: NO.31 :: Aug. 04, 2011
V. V. Subrahmanyam
�I am really enjoying the game now. Apparently, winning important titles makes so much of a difference to your attitude for the next big event,� she says.
�She is a special talent and has to be nurtured very carefully. The best part is she is young and willing to keep improving every day. And what impresses me the most is she never complains even if asked to do the extra hour of training on any given day,� says Gopi Chand.
It is heartening that neither Sindhu nor Gopi feel the job is over. �It is mission half-accomplished. If you want to be a force to be reckoned with at the highest level, two things are imperative � peak fitness levels and the ability to keep improving and adapting to the given conditions,� says Gopi. And, he ensures that the focus is clearly on these two aspects as one of his most gifted trainees is gearing up for the huge challenges.
�I am not in a hurry. I just want to take it event by event. You can always dream big but you should also have your feet on the ground. So, I don't talk big but am just keen to move up the ladder and take things as they come,� says Sindhu...
Does she feel the pressure of being tipped as the next Saina Nehwal? �No, not at all. The simple reason is that I never think on those lines despite she being my role model and a great source of inspiration. In fact, it is an honour and a privilege to train with her at the Academy,� says Sindhu...
Sindhu sweeps title in All-India Juniors
Gold rush for PV Sindhu
Hyderabad | Sunday,Aug,7,2011 IST
Full report: http://news.webindia123.com/news/Art...7/1807749.html
It was gold rush for India s latest sensation P V Sindhu as she won four titles at All India Major Junior Ranking Badminton Tournament that concluded at Filmnagar Cultural Center here today. P V Sindhu, continuing the good run after her recent triumph in Indonesia and Maldieves, almost swept all the four titles she fought. Having won the girls under -17 singles and doubles, Sindhu repeated the same performance in the under -19 girls category. It was a nice feeling to sweep all the titles Sindu said. Except big resistance she encountered in the under-19 semifinal against top seed Saili Rane, it was fantastic run of victories in the entire tournament, she added.
Sindhu looks to improve defence
Posted: Sat Aug 20 2011, 04:08 hrs Lucknow:
Full story: http://www.indianexpress.com/news/si...efence/834616/
After a brief stint at the Uttar Pradesh Badminton Academy, rising India shuttler PV Sindhu is all set to lead the Indian challenge at the Vietnam Open Grand Prix starting Monday.
And Sindhu, who has been playing continuously for the past few months, is happy at getting some quality practice time ahead of the rest of the season.
"Lucknow has been lucky for me because it was here that I was able to win India's first medal at the Badminton Asia Youth Under-19 Championships in July (she won a bronze). Just after that, I won the Indonesia International Challenge. I am working hard and I just hope to do well in the tournament at Vietnam. I will be rejoining the camp at Lucknow after that," she told The Indian Express before leaving on Friday night.
Immediately after the Vietnam Open, Sindhu will proceed for the Commonwealth Youth Games to be held at Isle of Man next month.
The Vietnam Open GP should be a good test of Sindhu's powers of concentration, and patience, and her adaptibility.
She meets Shih Han Hung of Chinese Taipei in round 1. If she wins it, she will probably meet 6th seed Aprilla Yuswandari of Indonesia next. If she manages to win there, she will probably be up against 3rd seed Tai Tzu Ying of Chinese Taipei in the quarter-final round! These promise to be interesting and tough matches for her. Lets see how she fares...
Sindhu v/s Firda at the recent Indonesia International Challenge.
Highlights in slightly speeded-up playback from PBDjarum.
Sindhu continues her impressive form at the Vietnam GP Open 2011. She just knocked off 6th seeded Aprilla Yuswandari 21-18, 21-19.
Next up is a big one for her: she runs into Tai Tzu Ying in the quarter-finals round! It will be an interesting clash of styles, and a test of her composure.
Update: Sindhu lost out to Tai Tzu Ying in 3 games, 11-21 21-11 21-19
No more details at this time, but hopefully, we can get some more info on this game soon...
Notch another one up for "Giraffe" Sindhu!!!
Sindhu has just won the Gold medal at the Commonwealth Youth Games 2011, hosted at the Isle of Man.
Sindhu was apparently in control throughout the tournament; she cruised through all her matches in sraight sets. The only hiccup came in the form of W. Tsai of Canada, who extended Sindhu to 3 games. But that again, might have been the rsult of her havin gto play in the Women's Doubles as well on that day.
Congratulations to Sindhu!
Badminton gold for Sindhu
The Hindu, HYDERABAD, September 12, 2011
P.V. Sindhu of India won the women's singles gold in the Commonwealth Youth Games badminton championship in Scotland on Sunday with a 22-20, 21-8 win over Sonia Cheah of Malaysia in the final, according to information received here.
This is the first major gold at the international level for the Hyderabad shuttler, sponsored by BPCL and Olympic Gold Quest. �I am delighted to win this title. I dedicate this gold to you (parents) and my coach Gopi Sir,� Sindhu told her parents P.V. Ramana and P. Vijaya (both former international volleyballers) � from Scotland.
India's chief national coach Pullela Gopi Chand felt it was a remarkable win by the 16-year-old shuttler.
�This shows her growing maturity in playing big events. No doubt, she is one of the favourites from India to emulate what Saina Nehwal has achieved in the big league,� Gopi said.
She's really good, from the video. Wonder how tall is she?
Elsewhere Sindhu was quoted on her showing at the CYG as saying:
"I am feeling great on winning my first women's gold in an international event. And that it came in the CYG is all the more special, for I put India in the gold medal winners' list," said Sindhu on arrival in Hyderabad.
..."I really enjoyed the CYG competition. Except for the first round match, where I took time to settle down, I was never uncomfortable. Everything fell in place. In fact, what pleased me was the way I attacked, engaged my rivals in long rallies and, more importantly, showed good stamina in Scotland," said Sindhu, who is sponsored by BPCL and Olympic Gold Quest.
..."I am not sure whether I will play in the next CYG because I might just cross the age limit. So even in this context the gold in Scotland is memorable. But right now I am keen on improving my defence in order to be a much better player," said Sindhu.
What next?
"I will be training hard in preparation for the World Championship (under-19) in Chinese Taipei next month. That should be a real big challenge," said Sindhu.
Read the full story here:
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Video: A Dark-n-Stormy Day in Brooklyn
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On the day Sandy hit, reporter Sally Herships went out into the streets of Brooklyn for a last-minute look at consumers' last minute preparations.
Defending yourself against fraud after a disaster
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Disasters leave people vulnerable to more than just damage on their homes. After hurricanes and other crises, fraudsters hit the streets -- and the Internet -- to try to cheat people out of their money. How can you protect yourself?
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A natural disaster like Sandy can be devastating whether you're wealthy or just getting by. But the rich could have an easier, faster recovery.
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Reaction to Libyan attack panned
Surviving Sandy in Brigantine, New Jersey
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There is no normal in New Jersey this week: Evacuation orders prevent some from returning home and Atlantic City's bright lights shine, while stop lights are dark.
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1970 Dodge Challenger - Pro Rodding
Written by on July 1, 2010 By Combining Pro Touring With Street Rodding, The Roadster Shop Has Upped The High-End Muscle Car Ante.At first, the reservation with which Jeremy Gerber speaks about his utterly wicked '70 Challenger seems a bit peculiar. After all, this is a machine encased in artfully sculpted sheetmetal that packs a 730hp all-aluminum Hemi, a Tremec five-speed, humongous brakes, and a trick independent rear suspension. As our conversation continues, it suddenly hits us. That's not a lack of enthusiasm in his voice, but rather an aura of quiet confidence-the kind of confidence that can only come from playing in a different pantheon of competition. Along with his dad and his brother, Jeremy spends his days building high-end street rods at the family business, the Roadster Shop (www.RoadsterShop.com). Although enthusiasts often criticize street rods as nothing more than outrageously expensive manifestations of rich guys' egos, there's no question that they represent the pinnacle of hot rodding craftsmanship. Applying those same skills to a muscle car has created quite a machine, one that exudes the same understated confidence as the crew that put it together.
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From every angle, the street rodding influence is resoundingly evident. Fastidious attention to detail oozes from every nook and cranny, and all the custom contours and crevices of the sheetmetal conspire to serve a single, dedicated purpose. At the nose, the Challenger has been fitted with a custom lower valence, chin spoiler, and grille. The hood started out as a flat reproduction piece, but since it looked way too ordinary, the Roadster Shop cut out the middle and molded in a recessed scoop. Beneath the vent, a custom air snorkel crafted from sheetmetal draws cool air and feeds the carb. Reshaping the rear profile is a custom lower roll pan, a slick center-outlet exhaust, a ducktail rear spoiler, and a tailpanel that narrows the taillights down to two tastefully thin slits. Furthermore, the front and rear bumpers have both been narrowed and flush-fit to the body, and the handles, trim, mirrors, and driprails have gotten a clean shave.
Topping off the visual tweaks are a set of halo-eyed headlights heisted off of a BMW, and a conservative black-on-gray paint scheme. The overall look is both menacing yet subdued, which is exactly what the Roadster Shop had in mind. "Challengers are such sexy-looking cars to begin with, so instead of completely changing the appearance of the car, we just wanted to make it look cleaner, and more modern and refined. The idea was to build upon the lines of the factory design, and turn it up a notch," Jeremy says.
"The car's color combination and graphics are totally out there, and people tell us that it looks like we sprayed clearcoat right on top of primer. The gray is actually some leftover paint we had from the very first roadster that we built. Most two-tone paint schemes have bright colors with lots of contrast. We decided to take a more low-key approach, and left the roof, trunk, and tops of the quarters the same color as the rest of the body."
Not to be overshadowed by the sultry skin, the Challenger's mechanical pieces are equally as impressive. Between the framerails sits a 528ci Indy Cylinder Head crate Hemi that spits out 730 hp and 660 lb-ft of torque. It's based on an Indy aluminum block bored to 4.500 inches, an Eagle 4.150-inch steel crank and rods, and Wiseco 10.25:1 pistons. Matched with a COMP 248/254-at-.050 mechanical roller cam, a Barry Grant 850-cfm carburetor, and Indy 440-cfm cylinder heads and intake manifold, the combo is both heroically nostalgic and potent. "It sounds absolutely wicked. The only appropriate motor for a Mopar is a big, naturally aspirated Hemi," Jeremy says. Moving farther rearward, the massive elephant mates to a Tremec TKO five-speed stick, which dispatches torque back to a Heidts independent rear suspension. The slick setup was originally designed by Heidts for street rods, then later adapted for muscle car applications. It's both simple and effective, and features a Ford 9-inch centersection mounted to the frame with a crossmember, 2-inch halfshafts, tubular lower control arms, aluminum spindles, QA1 coilovers, and inboard-mounted disc brakes.
Proving that its superb crafting skills aren't just limited to shaping sheetmetal, the Roadster Shop put on a veritable fabrication clinic beneath the car as well. Cradling the Hemi is a custom-built tubular K-member, which anchors custom control arms and aluminum spindles. A NASCAR-style splined front sway bar keeps body roll in check, and custom subframe connectors-triangulated at several points-run the length of the frame. Likewise, the exhaust system is more art than mere utilitarian piping. The elegant river of stainless steel tubing starts with custom 21/8-inch long-tube headers, which flow into 4-inch collectors and X-pipe. From there, dual 3-inch intermediate pipes tuck tightly into the trans tunnel before feeding Flowmaster mufflers, negotiating the IRS, hugging the sides of the gas tank, then merging together once again while exiting into the custom roll pan. Since band clamps and saddle clamps are just too ordinary, fancy V-band clamps join the tributaries together. "Even though this car sits very low, the exhaust is tucked in so nicely that nothing hangs less than 5 inches off the ground. This is very important, since driveabilty is a top priority," Jeremy says. "Unfortunately, it was the middle of winter when we finished this car, but we couldn't wait to drive it so we took it out anyways. It was a riot trunkin' it through the streets of Chicago during the middle of a snow storm. I can't wait for some nice summer weather so I can cruise it and run it on the autocross."
The Roadster Shop has proven once again that applying street rod savvy into building a muscle car yields one heck of a street machine. So why would a well-known street rod shop venture into the world of muscle cars? Jeremy says it's an escape from the stresses of building gazillion-dollar roadsters for the sole purpose of winning a trophy. "We started out as a chassis shop building frames for '32 Fords, then transitioned into building complete cars. However, indoor car shows aren't for everyone, so we're now taking on more and more muscle car projects," he says. "When it came time to build our own car, we wanted to build something that was over the top, but still completely streetable, functional, and comfortable. Personally, I have more fun building muscle cars because if you plan on running them hard, they have to be overbuilt and over-engineered to handle the rigors of abuse. As a car builder, I really enjoy that challenge."
For Jeremy and the rest of the Roadster Shop crew, the Challenger isn't their first crack at building a street rodded muscle car. Its LS7-powered '62 Corvette, dubbed C1RS, took home the 2009 Goodguys Street Machine of the Year award, and ripped up many an autocross course along the way. For seasoned street rod veterans like Jeremy, this muscle car stuff must seem way too easy. That's why if you ever start talking to him about his Challenger, you'll probably sound more excited about it than he does. While he most certainly appreciates what he's accomplished with the car, he also hails from a different league, where the typical best-in-show caliber muscle machine wouldn't even garner a second look on the indoor car show circuit. More than anything, that explains why the man is so confident, and why his cars reflect that aura of understated confidence. After all, as impressive as a 450-foot Albert Pujols home run may be, how excited would Pujols get about those long balls if he hit them off of minor league scrubs throwing 85-mph heaters?
Jeremy Gerber • Mundelein, IL | |
Type: | Chrysler 528ci Hemi |
Block: | Indy Cylinder Head aluminum |
bored to 4.500 inches | |
Oiling: | custom Indy dry sump with Moroso pan |
Rotating assembly: | Eagle 4.150-inch steel crank |
and 6.860-inch rods; | |
Wiseco 10.25:1 pistons | |
Cylinder heads: | Indy 285cc aluminum castings |
with 2.25/1.94-inch valves | |
CNC-ported to flow 440 cfm | |
Camshaft: | COMP 248/254-at-.050 |
mechanical roller; | |
.604/.591-inch lift; | |
110-degree LSA | |
Valvetrain: | COMP lifters and timing set; |
Indy 1.6:1 shaft-mount rockers, | |
Induction: | Indy 426-2 single-plane |
intake manifold, | |
Barry Grant 850cfm carburetor | |
Ignition: | MSD billet distributor, |
6AL ignition box, coil, | |
and plug wires | |
Fuel system: | Rock Valley Tank, Aeromotive pump |
Exhaust: | custom 21/8-inch long-tube headers |
and 4-inch X-pipe; | |
dual 3-inch Flowmaster mufflers | |
Cooling: | Edelbrock water pump, |
AutoRad radiator | |
Output: | 730 hp at 6,600 rpm |
and 660 lb-ft at 4,800 rpm | |
Built by: | Indy Cylinder Head (Indianapolis, IN) |
Transmission: | Tremec TKO 600 five-speed, |
Centerforce clutch | |
Rear axle: | Heidts independent rear suspension |
with Ford 9-inch centersection, | |
3.90:1 gears, and aluminum halfshafts | |
Front suspension: | custom tubular K-member |
and control arms; QA1 coilovers | |
Rear suspension: | Heidts IRS assembly |
and tubular control arms; | |
QA1 coilovers | |
Brakes: | Baer 12-inch discs with |
six-piston calipers front and rear | |
Wheels: | Budnik Gasser |
19x9 (6-inch backspace), front; | |
22x12 (6.5-inch backspace), rear | |
Tires: | Pirelli P-Zero |
255/30R19, front; | |
335/25R22, rear |


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Nightlife in Ho Chi Minh City, Vietnam -- Sightseeing
Easy to find a Rooftop Garden Bar for drinks where you can watch the sunset over the busy streets where the flicker of streetlights and neon begin to come to life.
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Work begins on demolition of harbor facilities
by Associated Press Published: November 14,2013
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OCEAN SPRINGS — Demolition has begun on a vacant boat storage shed and a restaurant will be come down next at the Ocean Springs Harbor. The Sun Herald reports that Southern Recycling and Demolition Inc. of D'Iberville has 90 days to do the work. Thomas Hickman, owner of Southern Recycling, said once workers get the […] [...]


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Model 7363 | Condition Factory New |
Bud's Item # 97363 | UPC 047700273631 |
New In Box. Part Number:7363 Buds Item Number: 97363 Remington 30-06 Springfield Special Purpose Synthetic w/Blue Barrel The Model 700 SPS offers the unrivaled out-of-the-box accuracy and high-end performance you've come to expect from America's most popular bolt-action centerfire rifle.The Model 700 SPS features an improved, ergonomically designed synthetic stock for better handling and enhanced overall functionality. The carbon steel barrels are clean without sights and receivers are drilled and tapped for scope mounts. All exterior metalwork features a matte blued finish. Standard amenities also include the revolutionary recoil reducing SuperCell recoil pad, a hinged floor plate magazine, and swivel studs. SPECIFICATIONS: Mfg Item Num: 7363 Category: FIREARMS - RIFLES Action :Bolt Action Caliber :30-06 Springfield Barrel Length :24" Capacity :4 + 1 Trigger :Single Stage Safety :Two Position Length :44 1/2" Weight :7 3/8 lbs Stock :Black Synthetic Finish :Blue
*Please Note!! Many of our pictures are stock photo's provided to us by the manufacturer and do not necessarily represent the actual item being purchased. Please verify this picture accurately reflects the product described by the title and description on this page before you place your order.


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smart for-us concept puts a mega-pickup spin on the fortwo
Article Summary
Much as some people loathe it, I've always kind of liked the Subaru Baja. It has the quirky short-bed car/truck look of the Hummer, but isn't anywhere near as large and nasty as that vehicle. Well, the designers at smart have taken the "little Hummer" idea to the extreme, with the for-us concept. Premiering next month at the North American International Auto Show, the two-seater electric car looks pretty much like a smart fortwo ... with a little cargo bed in back.
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Fees & Graduate Funding
Welcome to the Fees & Graduate Funding homepage. Here you will find information on funding opportunities, scholarships and bursaries, student loans and fees for current students administered by the Student Registry's Fees and Graduate Funding team.
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Welcome to the Town of Leesburg Parks and Recreation Department. We are pleased to provide a variety of programs, facilities, and services to meet your recreation and leisure needs. Of the 16 parks, Ida Lee Park is the crown jewel of the department, featuring a recreation center with 71,000 square feet of aquatic, fitness, and recreation opportunities, as well as an 11-court tennis complex and outdoor aquatic center. Each year we offer over 3,000 recreation programs, which include programs for all ages and we have over 370 acres of parkland for the outdoor adventurer. We are proud to say we are a Two Time National Gold Medal Award Winner for Excellence in the Field of Park and Recreation Management and we take pride in continuing to offer award winning programs and facilities to meet the diverse needs of the Town of Leesburg citizens. Whether you are new to the area, or a lifelong resident, come and see what makes us award winning. Most of all, make time to recreate with us!
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