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Golden State Warriors
Golden State Warriors Tickets
The Bay Area is ready for another season of exciting basketball with
Golden State Warriors Tickets
! After all, where else can you find the best basketball players in the world, dazzling the crowd with no-look passes, spectacular slam dunks, and three-point daggers. This is the NBA at its finest and you'll want to grab your Warriors tickets as they battle Pacific Division rivals Lakers, Suns, Kings, and Clippers. Do the Golden State Warriors have the talent to bring a title back to Oracle Arena? There's only one way to find out. Razorgator has the best selection of Golden State Warriors Tickets for all home and away games. Check out the Warriors schedule and purchase your warrior tickets today.
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May 13
NBA Western Conference Semifinals: Grizzlies at Warriors - HG 3 - Game 5
Oracle Arena
May 17
NBA Western Conference Semifinals: Grizzlies at Warriors - HG 4 - Game 7 (If Nec.)
Oracle Arena
May 19
NBA Western Conference Finals: TBD at Warriors - HG 1 (If Nec. - Date TBD)
Oracle Arena
May 21
NBA Western Conference Finals: TBD at Warriors - HG 2 (If Nec. - Date TBD)
Oracle Arena
May 28
NBA Western Conference Finals: TBD at Warriors - HG 3 (If Nec. - Date TBD)
Oracle Arena
Jun 01
NBA Western Conference Finals: TBD at Warriors - HG 4 (If Nec. - Date TBD)
Oracle Arena
Jun 04
NBA Finals: TBD at Warriors - HG 1 (If Nec. - Date TBD)
Oracle Arena
Jun 07
NBA Finals: TBD at Warriors - HG 2 (If Nec. - Date TBD)
Oracle Arena
Jun 14
NBA Finals: TBD at Warriors - HG 3 (If Nec. - Date TBD)
Oracle Arena
Jun 18
NBA Finals: TBD at Warriors - HG 4 (If Nec. - Date TBD)
Oracle Arena
Golden State Warriors 2014-2015 News and Updates
5/5/15 - After dispatching the young upstart Pelicans in the first round of the NBA playoffs, Stephen Curry and his Golden State Warriors advanced to Round 2 to face the Grizzlies. Golden State made quick work of New Orleans with a four-game sweep, and seem to have maintained their momentum with a 101-86 victory over Memphis in Game 1 of their Western Conference Semifinals matchup. To no one's surprise, Curry led the way with 22 points and seven assists, and Klay Thompson added 18 and six to help propel the Warriors to a 1-0 series lead. Golden State will look to defend their home floor again on Tuesday night as they host the Grizz in Game 2 of the second round. Tickets for Oracle Arena are available from $137 but they won't last long!
4/30/15 - The Golden State Warriors continued their winning streak all the way to the end of the playoffs' opening round. It is no surprise that the top team in the league were able to beat out the New Orleans Pelicans, who secured their spot in the postseason on the final day of the season. Remaining undefeated is, however, still an impressive feat, only two other teams also pulled it off. The Warriors' opponents in the conference semifinals has also been determined: the Memphis Grizzlies. While they are still only the number five seed, the Grizzlies will put up a much tougher fight than the Pelicans. Will the Warriors be able to remain undefeated? Find out at the first game of the second round, on May 3th.
… more
4/22/15 - The Golden State Warriors just finished up the second playoff game, and they came away with two victories. No one expects less from the team leading the entire NBA league. Despite their spot on the top, they did only win the opener by seven points, however, which is less than many expected. While they scored less overall, they held a stronger lead in their second game. For a team that only managed to secure a spot in the postseason on the final day of the regular season, the New Orleans Pelicans are putting up a very impressive fight. Fans will have to wait and see how the Warriors fare once they get on the Pelicans' home court. Most suspect they won't have any trouble taking two more victories, but they cannot underestimate New Orleans.
4/16/15 - As everyone suspected, the Golden State Warriors were able to continue their winning streak up to the final game of the season. Being the number one seed for the Western Conference and the top team in the entire league, the Warriors have a very realistic chance to take the championship. First up, they will be taking on one of the lowest ranked teams, the New Orleans Pelicans, who have more than double the losses as the Warriors. While the Pelicans were able to just barely qualify for the playoffs, the Golden State cannot let their guard down. They will be taking on the Pelicans on their home court, so you will have an opportunity to see the playoff opener live. Tickets are still available starting at $134.
4/6/15 - With the wire approaching, the Golden State Warriors fell flat on the road against the defending NBA champions from San Antonio, 107-92. The loss snapped a 12-game winning streak for the Warriors, and was just their second loss in the last 14 games; still, Stephen Curry hit for 24 points.
3/30/15 - The streaking (it feels like we've used that adjective all season for them) Golden State Warriors have officially clinched the top spot in the Western Conference, and are well on their way to earning home-court advantage throughout the playoffs. The Warriors, who have won nine straight in their last 10 games, hold a 10 game lead over second place Houston.
3/23/15 - Well, the Western Conference has its first playoff team, and it's no surprise that it's the Golden State Warriors, who have been dominant since the start of the season. The Warriors currently hold the best record in the league (56-13, for an .812 winning percentage), and are 7 ½ games clear of the Southwest Division leaders, Memphis.
3/16/15 - With a win against the Lakers Monday, March 16, the Golden State Warriors continue their winning season with a record of 53-13, which puts them ranked first in the Pacific Division. With 26 points from Klay Thompson and 19 points and nine assists by Stephen Curry, the Warriors secured a position in the playoffs, with help from Oklahoma City's loss to Dallas. However, Thompson will be out for the next week to 10 days after spraining his ankle during the Lakers game. This season marks the third in a row that the team will head to the playoffs, but the team is not satisfied. They are striving to finish the season with the NBA's best record. The next step will be a victory over the Atlanta Hawks on March 18. Tickets remain on sale, starting at $33.
3/9/15 - According to John Hollinger's power rankings formula for ESPN, the Golden State Warriors are tops in the league in the probability that they will win the league title, at a healthy 38.6% chance, which is more than double the next highest team, Cleveland in the East. Still, championships are never won on paper, and there's still a lot of hardwood between now and June.
2/23/15 - Percentage-wise, the Golden State Warriors have resumed their place at the top of the entire league. The Warriors, who lost their last time out, have lost just three times in the last 10 games, and their 43-10 record puts them at a winning percentage of .811, 25 percentage points ahead of Atlanta, the surprise best team in the East.
2/18/15 - The Golden State Warriors kick off the two-month, post-All Star stretch drive at home against San Antonio this weekend, before hitting the road for a six-game Eastern swing. As expect, Steph Curry outdueled his Splash Brother teammate Klay Thompson en route to a Three-Point Contest victory.
2/9/15 - Still the best team in the Western Conference, the Golden State Warriors have won three of four in the month of February. After falling on the road to Eastern Conference leader Atlanta (despite Klay Thompson's game high 29 points), the Warriors bounced back with a solid thumping of New York.
2/2/15 - The Golden State Warriors have cooled off a big from the scorching-hot start they got to the season. Still, the Warriors are 7-3 in their last 10 games, and hold a solid 5 ½ game lead over the L.A. Clippers at the moment.
1/26/15 - Golden State Warriors guard Stephen Curry was not only named as a starter to the All-Star Game for the Western Conference, he led all players in voting. Curry, who is leading the Warriors to one of the best seasons in the team's history so far, outgained Cleveland's LeBron James for top vote-getter this season.
1/19/15 - After falling last weekend on a visit to Oklahoma City – a loss which snapped a six game winning streak – the Golden State Warriors picked right back up again. The Warriors prevailed in their next two games, collecting wins over Houston and Denver.
12/22/14 - After having their 16-game winning streak snapped last week by Memphis, the Golden State Warriors set themselves back into winning just two days later. The Warriors hung a five-point victory on visiting Oklahoma City, 114-109, to get back into victory mode.
12/15/14 - Meanwhile, in the Bay Area and elsewhere, the Golden State Warriors just keep on winning. The Warriors haven't lost since November 11, when San Antonio upended them, 113-100. In fact, that was the second of the two losses the Warriors have incurred, the other being two days earlier at Phoenix.
12/8/14 - Can anybody stop the Golden State Warriors? At this point, it's looking mighty difficult; the Warriors are in the midst of a 12-game winning streak, the longest streak in either conference at the moment, and are outscoring their opponents by almost 11 points per game on average.
12/1/14 - Talk about a team on a hot streak. That's the Golden State Warriors, who continue to be one of the top teams in the league; the Warriors have a nine-game win streak in progress that is currently the league's current longest.
11/25/14 - With a five game winning streak – tied with Toronto for the second-longest in the league, behind Portland) the Golden State Warriors are starting to separate themselves from the pack in the Pacific Division. They're 2 ½ games ahead of the second-place tie of Sacramento and the L.A. Clippers, and their .857 winning percentage is also second leaguewide, behind Memphis and Toronto.
11/17/14 - The Golden State Warriors overcame a 44-point outburst from Kobe Bryant en route to a solid 136-115 weekend victory over the struggling L.A. Lakers. Stephen Curry dropped 30 points and 15 assists on the Lakers, while Andrew Bogut collected 10 rebounds.
11/10/14 - The lack of Splash Brother Klay Thompson – out with a sprained hand – may or may not have been a deciding factor in the Golden State Warriors' first loss of the season, to division rivals Phoenix. This came just after the Warriors dealt Houston their own initial loss this year.
11/3/14 - There's three unbeaten teams in the Western Conference…and one of them is the Golden State Warriors. The boys from NorCal are on a roll, as evidenced by Klay Thompson's 41-point outburst against a weak Los Angeles Lakers squad over the weekend.
8/18/14 – The 2014-2015 Golden State Warriors schedule was unleashed last Wednesday and, with last year's playoff team left intact, much anticipation is building on the 82-game slate. How will this high-scoring team fare with a new coaching staff? The Warriors open up their season on the road with a game against the Sacramento Kings on Oct. 29. Rookie head coach Steven Kerr's big debut home opener is set on Nov. 1 against the Los Angeles Lakers at Oracle Arena. Recall the blood-sweat-and-tears Game 7 of Round 1? Buy Golden State Warriors tickets now and be in the stands for the rematch everyone is itching to see. On Nov. 5, the Warriors play their second home game against the visiting Clippers, while Christmas Day will see the Steph Curry-led squad down in Los Angeles, for another game against Blake Griffin and company.
For the second year in a row, the Golden State Warriors earned the No. 6 seed when it came time for postseason. In round one's Battle of California, the Clippers, who proved undaunted by the media frenzy regarding their owner, dispatched the Warriors after a white-knuckle Game 7. Golden State grappled with their fair share of drama off the court last year as well. Coach Mike Jackson's hot seat reached a boiling point when assistant coaches Scalabrine (reassigned to a D-Leage position) and Erman were dismissed. Despite all of this, the Warriors were fire on the court. Stephen Curry further established himself as a superstar in the NBA, while Klay Thompson stunned all with his shooting capabilities; Draymond Green proved to be a top forward prospect and Andrew Bogut played a lengthy season as defensive powerhouse.
Can Golden State surpass last year's early exit with less distraction from their front office? The Warriors are revived for the 2014-2015 season, touting new head coach Steven Kerr, acquiring Shaun Livingston as backup point guard, as well as former Warrior Brandon Rush, who will ramp up the bench. The franchise was left with zero draft picks in the offseason and, as such, are returning a familiar squad. Are you ready for more action from the Splash Brothers and company? Buy your Golden State Warriors tickets now and be in the stands when this improved team hits the courts in 2015!
2013-2014 Season
5/2/14 - Lost in all the off-court hoo-haw over the Los Angeles Clippers the last several days is the fact that they are being challenged by a very good team – the Golden State Warriors. Though the Warriors took advantage of home atmosphere and a clearly distracted opponent in Game 4 this past weekend, Golden State found themselves on the short end in Game 5, falling by 113-103 and down 3-2 in the series. While sharpshooters Steph Curry and Klay Thompson have been as-advertised all series, one veteran name which has seen more ink than it has in a while, center Jermaine O'Neal, fell off a bit in Game 5, scoring just eight points and pulling down five rebounds. Golden State reserves scored just 19 points, while O'Neal was the only reserve to make a presence felt under the glass.
4/17/14 - Bad luck for Golden State. Before their last game, a 130-120 comeback win over Minnesota, center Andrew Bogut was pulled from competition due to a broken rib. The San Francisco Chronicle reported that Bugut initially suffered the injury in a loss to Denver, then had it made worse in the fourth quarter of an overtime loss to Portland. The break is apparently very close to Bogut's lung, and playing further would risk puncturing the lung, Bogut told the Chronicle. Even without their big man, the Warriors still had enough weapons to handle Minnesota; Stephen Curry lit up the T-Wolves for 32 points, 15 assists, and five rebounds, while David Lee had 25 points and nine boards, and Klay Thompson put up 20 points, as did reserve Draymond Green. Expect to see more of veteran Jermaine O'Neal in the middle for the Warriors; O'Neal collected 10 points, four rebounds and a steal against Minnesota.
4/10/14 - One would think with the Golden State Warriors being on the brink of clinching a playoff spot in the highly competitive Western Conference, that all would be sunshine and roses around Oracle Arena. Not according to the San Francisco Chronicle, who recently wondered in print whether Warriors owner Joe Lacob might be running out of patience with head coach Mark Jackson. The Warriors have lost one assistant coach and demoted a second; and even though the squad is on the doorstep of its first 50 win season in 20 years (currently at 48 wins), several key players including Andrew Bogut, David Lee and Andre Iguodala are less than completely healthy as the NBA Playoffs approach rapidly.
4/2/14 - His stroke is featured in the latest ESPN the Magazine, how he is reinventing the art of shooting the basketball. So it was no surprise – or April Fool's gag – that the Golden State Warriors' Stephen Curry dropped a game-winning 20-footer with just over two seconds left in overtime to give the Warriors a clutch 122-120 win in Dallas. Curry, with 23 points and 10 assists, was one of three Warriors who broke the 20-point plateau; Klay Thompson led Golden State's scoring with 27 points for the evening, while veteran center Jermaine O'Neal combined 20 points with eight rebounds, including the key defensive board that led to Curry's gamer. Jordan Crawford provided 19 points off the bench, while Andre Iguodala chipped in 16 points and eight rebounds.
3/26/14 - After winning three straight, two at home, the Golden State Warriors fell to conference leading San Antonio, 99-90, last weekend, also at Oracle Arena. The Warriors were unable to use a 54-40 advantage in rebounding to their benefit; the Warriors' battle of the boards was led by Andrew Bogut's 17; Bogut also had four blocked shots, while his offensive output was negligible at just two points at 1-of-7 shooting from the field. Stephen Curry led the Warriors in scoring with 20, while backcourt mate Klay Thompson added 16. Forward David Lee is hoping a strained hamstring will allow him to take the court this weekend when next the Warriors play, against Memphis.
3/19/14 - After losing unexpectedly at home to Cleveland, the Golden State Warriors pulled back together, earning a tough 113-112 overtime win on the road in Portland, then coming back to Oracle Arena to easily handle Orlando, 103-89. Even though the Warriors were without a pair of key players in Andre Iguodala and Andrew Bogut, starters Stephen Curry (23 points), David Lee and Klay Thompson (20 points each) took care of business for Golden State. Lee was tops on the squad with 10 rebounds while Draymond Green collected nine. Iguodala is expected to be out for another few games, suffering from tendinitis in the right knee.
3/13/14 - Winners of their last five straight, the Golden State Warriors Are solidifying themselves as a playoff team. After finishing an Eastern road trip with a pair of wins (at Indiana and Boston), the Warriors have knocked off Atlanta, Phoenix, and Dallas; and only Phoenix came close (113-107), but the Warriors used a 16-0 third quarter run which put them up by 15. Stephen Curry finally got a little rest for his sore quadriceps muscle in his leg after scoring 19 points in 30 minutes against Phoenix; he played just 22 minutes in the most recent win over Dallas. Jordan Crawford, with 19 points off the bench, led Warriors scorers, while frontcourt starters David Lee (11) and Andrew Bogut (10) led in rebounding.
3/6/14 - Golden State bolstered their resume as a legitimate title contender on March 4, becoming the only Western team to knock off the East's top two teams, Indiana and Miami, on the road. The Warriors slipped past the Pacers at Bankers Life Fieldhouse, 98-96, on Klay Thompson's 12-footer with less than a second on the clock. Thompson's 25 points were a team high for the Warriors; Stephen Curry contributed 19 points, eight rebounds and six assists in the winning effort as well. Center Andrew Bogut's offensive contribution was minimal – three points – but Bogut recorded three blocked shots and six boards.
2/27/14 - Golden State started a long road trip through the Eastern Conference on the right foot, defeating the Detroit Pistons 104-96 at the Palace of Auburn Hills. Stephen Curry naturally led the Warriors in scoring, collecting 19 points along with Klay Thompson. Big man Jermaine O'Neal collected 16 points and 10 boards along with three blocked shots as an anchor for the Warriors' defense. Speaking of O'Neal, the San Francisco Chronicle newspaper recently noted that O'Neal called a team meeting shortly after the All Star break, and credited his speaking at that meeting with breaking the Warriors "out of their inconsistent plod through the end of January and into February."
2/21/14 - Golden State guard Stephen Curry had a bit of a disappointing time at the NBA's All Star Weekend last weekend. Curry failed to advance out of the first round of the three point shooting contest, ultimately tying Kyrie Irving from Cleveland and Minnesota's Kevin Love. As a starting guard for the West in Sunday's All Star Game, Curry scored 12 points but dished out 11 assists in the loss. However, back in action for the Warriors, Curry lit up the Miami Heat for 29 points and seven assists in a one-point loss to the defending champs.
2/7/14 - If you ask the Golden State Warriors sharpshooter Stephen Curry, the Warriors still have room for improvement. "I feel like we are underachieving right now," Curry told the
Oakland Tribune
in the aftermath of Golden State's 102-87 win over the Chicago Bulls on February 6. "I feel we can only control what's ahead of us." Ahead for the Warriors? Visits from Philadelphia and Miami before the All Star break; then a trip to Sacramento, followed by home tilts with Houston and Brooklyn.
1/31/14 - Is there another NorCal vs. SoCal sports rivalry developing? When it comes to the Golden State Warriors and the Los Angeles Clippers, the answer is clearly yes – just ask Warriors coach Mark Jackson, who told CSN Bay Area, "(Y)ou're jockeying for playoff position in the same division, it makes it exciting." When the two squads met in Oakland on Christmas night, multiple ejections ensued; but not so much on January 30, when Golden State handed their rivals a 19-point loss, never trailing at any time in the game.
1/10/14 – The Road Warriors run may be over as the Nets topped Golden State Wednesday, snapping the team's 10-game win streak and stripping them of an NBA record, but Stephen Curry and co. remain one of the hottest teams in the league. Game-winning buckets, a 70 point second half, and an improved defense are creating havoc for competitors. The next road game for the GSW is a must-see when they travel to Oklahoma City to take on the Thunder. Two of the league's most explosive and exciting teams will take the hardwood for the third time this season following two last-second buzzer beaters that have the Western Conference foes tied 1-1. The last time OKC came to "Roaracle", Andre Iguodala hit a fading jumper as the buzzer sounded to give the Warriors the win. 2014 Golden State Warriors tickets on the road are averaging $199, $91 less than a ticket at Oracle Arena.
Warriors fans held their breath when Stephen Curry left the court last night walking gingerly. Said to have sat out the fourth quarter due to a bruised left foot and a sore right knee, the star guard is expected back shortly. Klay Thompson led the way in Curry's absence and the Warriors are now off to a 4-1 start on the season. The most indemand tickets at Oracle Arena this year, are games played against back-to-back NBA Champion,
Miami Heat
(2/12), Los Angeles Lakers (12/21), and
Brooklyn Nets
(2/22). Christmas Day in Oakland vs. Clippers is the fourth highest priced Golden State Warriors ticket this year. Compared to last season,
Warriors tickets are up 60%
, averaging $147 but remain $21 under the league average. On Tuesday the team announced that they would cap season-ticket sales at 14,500 and start a waiting list.
With one of the most exciting young teams in the NBA, the Warriors look to build on last season's unexpected second-round playoff run. The addition of veteran free agent Andre Iguodala adds playmaking and toughness to an already talented lineup featuring sharpshooter Stephen Curry, who broke the single-season record for 3-pointers last season. Second-year coach Mark Jackson and the team open their season on 10/30 at Oracle Arena against the Lakers. The schedule also features a
Christmas Day
matchup at home against the
Los Angeles Clippers
2012-2013 Season
2/20/14 - It's the world turned upside down. Traditional Western powers like the Los Angeles Lakers and Phoenix Suns are struggling for success, while longtime cellar-dwellers like the Golden State Warriors are reaching out for success and winning traditions, and so far, the Warriors are doing it mostly right. Even though they're currently in the midst of a losing streak, the Warriors are hanging in there in the thick of the playoff chase; they're getting the job done; sandwiched in between their division rivals in Los Angeles, even though they have a barely negative difference in points per game scored vs. points per game allowed.
Point guard Stephen Curry is not only getting the ball to his teammates — leading the team in assists per game — but he is also leading in scoring as well. Forward David Lee and shooting guard Klay Thompson are also valuable contributors to the Warrior cause, along with Harrison Barnes and Festus Ezeli. Jarrett Jack and Carl Landry provide valuable minutes off the bench when the situation warrants. Fiery coach Mark Jackson — a former
all-star point guard himself — needs to get his players working harder to improve their scoring defense if the Warriors are to have any postseason success at
Oracle Arena
this year.
Warriors tickets average $111 at Oracle Arena; the young team's success has made it one of the ten most watched home teams in the NBA this season. Cheap Warriors basketball tickets start at under $5 — one of the best NBA tickets deals in 2013. Surprisingly, Golden State's one of the most popular road teams too, with an average road game tickets price of $111. What are the best games on the 2013 Warriors schedule? Upcoming Golden State Warriors home games include matchups with the
San Antonio Spurs
Houston Rockets
New York Knicks
Chicago Bulls
(3/15), and
LA Lakers


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Protecting God's Children:
No child or adult should ever experience abuse by anyone, especially a church minister. We all have the right to be treated with dignity and to expect our church to reflect the goodness of God in all of its dealings.
We hope that this information is helpful to all in our communities who are working to prevent and respond to childhood sexual abuse. We have tried to be as clear as possible about our assistance to victims, reporting procedures, and education and prevention efforts. Please let us know if you need further assistance or information by calling or emailing our victim assistance coordinator, Ms. Martine Siegel, at 502/636-1044; email@example.com or the Chancery at 502/585-3291.
We must heed God's call to care for the most vulnerable among us and to continue our efforts toward wholeness and health in dealing with sexual abuse.
The Sexual Abuse Policies of the Archdiocese of Louisville |
Victim Assistance | |
Reporting Sexual Abuse | |
Sexual Abuse Review Board | |
Community Resources | |
Investigative Procedures | |
Prevention and Education/Code of Conduct for Church Personnel | |
Communication Policies | |
Definition of Terms | |
Restoring Trust Booklet-2013 Revision (PDF Format): In English In Spanish | |
Safe Environment Training |
Description | |
Training schedule | |
Resources for Parents |
Message to Parents | |
What To Do If Your Child Is a Victim of Sexual Exploitation | |
Reporting to Authorities | |
Center for Women and Families | |
National Center for Missing & Exploited Children | |
Local and National Resources | |
Related Resources/School and Religious Education Curriculum Materials | |
U.S. Catholic Bishops' "Charter for the Protection of Children and Young People" |
Click here to view the Charter | |
Archdiocese of Louisville Updates |
Click here to view updates issued from July 2002 through the present |

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President & CEO
Balboa Park's Museums & Science Centers span diverse interests, from fossils to photons, we have it all!
Balboa Park's Performing Arts organizations offer a wide variety of symphony performances, organ concerts, musicals, comedies and theatre productions!
Balboa Park's Cultural & Visitor Centers provide enriching artistic and cultural experiences for the people of San Diego and beyond!
Become a supporter today! Click here for more information.
The Balboa Park Cultural Partnership serves as the collaborative body and collective voice for 28 arts, science, and cultural institutions in Balboa Park. Together we achieve greater effectiveness, innovation, and excellence and contribute to the vitality and sustainability of Balboa Park. Our collective 500 trustees, 7,000 volunteers, and 3,500 staff serve more than 6.2 million visitors annually.
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text: Tempe, AZ – Gumby is celebrating the 60th birthday of his creation in style! Once a little green slab of clay, international icon, Gumby, came to life in 1955, through the creative genius of Art Clokey. Arizona State University's Gallery 100 is hosting "Gumby, Behind the Scenes: Celebrating 60 Great Years," an exhibit of Gumby memorabilia from Clokey Productions, to kick off the festivities. The show features real clay puppets used for animation and impressive full-scale » Read more


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Commercial Printing in Weston |
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Helpful Definition for: Commercial Printing
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Constructing a Vector for Over Expressing Nitrobenzene Dioxygenase in E. coli Paul Davis 5-13-11 Abstract: Due to extensive use of nitroaromatic compounds in the production of dyes, pesticides, explosives, and their harmful effects on the environment, studying the enzyme mechanisms of bacteria capable of utilizing these compounds as nutrient sources have become a recent field of development. One example, the Comamonas sp. strain JS765, grows on nitrobenzene as its sole carbon, nitrogen and energy source. This bacterium contains a nitrobenzene dioxygenase enzyme system that incorporates O2 into the nitrobenzene ring to form catechol and nitrite. In this project, the genes that code for the dioxygenase enzyme were cloned and then transformed into an expression vector for over-expression in E. coli NEB 5α and E. coli BL21 (DE3). This over-expression system will facilitate purification of the normal enzyme and of mutant forms for mechanistic studies. Introduction: Nitroaromatic compounds are commonly used as industrial feedstocks for the production of pesticides, dyes, and explosives.1 When these compounds enter the environment through improper storage and disposal techniques, they present a hazard to living organisms. One solution for properly digesting these compounds before or after they have been introduced to the environment has been the use of aerobic bacteria that are capable of degrading these compounds. Due to the resistance of aromatic compounds, such as nitrobenzene, the digestion of these compounds through the use of bacteria has been found to be specific to each bacterium rather than any one bacterium being able to digest a range of aromatic compounds. One such bacterium that is capable of digesting a nitroaromatic ring is the Comamonas sp. strain JS765. This bacterium digests nitrobenzene through a multicomponent dioxygense1, and it is the physical mechanism of this molecular degradation that is of interest in this study. The enzyme responsible for this degradation is nitrobenzene dioxygenase (NBDO), which is in the Rieske nonheme iron dioxygenase family. Rieske dioxygenases are a target of significant research for two main reasons; first, they incorporate two chiral centers adjacent to one another without producing stereochemical mixtures, and secondly they catalyze the first reaction in a pathway by which microbes break down aromatic compounds so that they can re-enter the global carbon cycle.2 The reaction by which NBDO breaks down nitrobenzene can be seen in Figure 1, which shows that NBDO Figure 1: Enzymatic reaction of NBDO, depicting the characteristic cis-dihydroxilation reaction of a Rieske dioxygenase.3 utilizes an O2 molecule from the atmosphere to oxidize the aromatic ring in a manner such that they are incorporated onto the same side of the ring. Also, an important environmental feature of this reaction is that it is converting nitrobenzene, an environmentally hazardous chemical, to catechol, which is activated by virtue of having two adjacent hydroxyl groups on the ring. The benzene ring of catechol is then oxidatively cleaved by catechol dioxygenases, and the products are subsequently degraded to common metabolites, such as intermediates of the citric acid cycle.2 When looking at the gene sequence that codes for the overall nitrobenzene dioxygenase system (NBDOS), it is composed of five particular sequences; nbzAa, Orf2, nbzAb, nbzAc, and nbzAd1. However, NBDOS is only composed of three proteins; NBDR (for reductase), NBDF (ferredoxin), NBDO (for oxygenase). The nbzAa gene codes for NBDR, an iron- sulfur flavoprotein reductase (NBDR), and the nbzAb codes for ferredoxinNBZ, a Reiske ferredoxin (NBDF). While both of these proteins are necessary for the NBDOS to function in vivo, it was the nbzAc and nbzAd genes that were of interest to this study. They code for oxygenaseNBZα, and oxygenaseNBZβ respectively, which are the α and β subunits of the NBDO enzyme. While it is unknown exactly how the NBDOS catalyzes the conversion of nitrobenzene to the corresponding cis-diol, a basic understanding has been established from other studies of Rieske dioxygenases. Two electrons are passed from the iron-sulfur flavoprotein reductase, which acts as an electron redox switch, to the Rieske ferredoxin which contains a iron-cluster capable of being reduced by the electrons.4 These electrons are then passed to NBDO, where O2 binds to the mononuclear iron center and becomes reduced to a (hydro)peroxo intermediate once nitrobenzene is introduced to the reaction. Two hypotheses have been put forward to describe how the oxygen-oxygen bond is cleaved so that the oxygen atoms can be inserted into nitrobenzene, as seen in Figure 2. One proposal is that the reaction proceeds through a concerted process where the (hydro)peroxo intermediate reacts directly with nitro benzene5, while the other is a two step process that forms a very reactive Fe(V) intermediate.6,7 Figure 2: Proposed reaction mechanisms for cis-dihydroxylation of nitrobenzene.3 In order to determine which of the two proposed pathways is correct, it is necessary observe the intermediates that are being formed during the reaction. There is one report5 of the detection of a peroxo-iron(III) intermediate by Mössbauer spectroscopy when a related Rieske dioxygenase was reacted with hydrogen peroxide and its substrate, but thus far neither this species nor the Fe(V) intermediate has not been detected during the reaction with O2 and aromatic substrate. We hope to improve the likelihood of observing a reactive intermediate through the use of mutant enzymes and/or alternate substrates designed to maintain the normal rate of formation but slow the rate at which the intermediate decays. One way in which this might be done would be to mutate the asparagine 258 residue of the oxygenase subunit.8 In a study by Parales et. al, a series of mutations were discussed, and it is the mutation from asparagine to valine at residue 258 CatecholNO2-Fe2+NO2Fe3+Fe2+SSO2Fe3+NO2Fe3+Fe3+SSOOH1Fe5+NO2Fe3+Fe3+SSOOH2Fe3+NO2Fe3+Fe3+SSOOHH22 e-(from NBDF)+ NitrobenzeneCatecholNO2-Nitrobenzenethat is of particular interest given our goal.8 The group used a starting reactant of 4-nitrotoluene and monitored the product that was made. In this case, the untreated protein produced 4-methylcatechol 95% of the time, as opposed to the mutant which produced 4-nitrobenzylalcohol 99% of the time (Figure 3).8 This difference in product distribution Figure3: Different products based on the 258 residue.8 indicates that the asparagine in the 258 position must have a significant role in the orientation of the incoming reactant, probably by way of hydrogen bonding to the nitro group of the substrate, because valine is incapable of such bonding (orientation of nitrobenzene in active site is displayed in Figure 4). It seems likely that the steps of the mechanism that generate the activated oxygen species in this mutant, such as the binding 4-5 991008060402004-MethylcatecholNBDON258V4MC9514NBA 99100806040200of substrates and the reduction of O2, would remain unchanged relative to the native enzyme. However, because the substrate is presented to the intermediate in a different orientation, it seems likely that the rate of reaction of the substrate with the activated oxygen intermediate will be affected, facilitating the observation of this intermediate through spectroscopic techniques. Figure 4: Orientation of nitrobenzene in the active site of NBDO.4 The goal of this project is to accomplish the initial preparations for carrying out large-scale productions of native or mutant proteins by cloning the nbzAc, and nbzAd genes into an expression vector and transforming them into E. coli (New England Biolabs, Inc. Cat. No. C2987I) NEB 5α and E. coli BL21 (DE3) for the over production of the α and β NBDO dioxygenase subunits. E. coli was selected because it is readily N258 Nitrobenzene 3.06 Å 4.9 Å 4.4 Å available, inexpensive, easier to grow in the laboratory than Comamonas sp. strain JS765, commonly used for molecular biology applications, and does not express its own native NBDO system. Materials and Methods: The Comamonas sp. strain JS765 used was grown in either Difco Nutrient agar and Difco Nutrient broth, or BLK agar and BLK broth. However, the conditions used for the BLK agar and broths were determined by the concentration of the nitrogen and carbon source nitrobenzene. BLK is a minimal media that lacks a carbon and nitrogen source,16 and in this study succinate was added to this media as the primary carbon source and nitrobenzene was used as the sole nitrogen source and secondary carbon source. The conditions used for bacterial growth was a nitrobenzene concentration of 0.750 mM for the broths, and the placement of a pipette tip filled with 0.750 mM nitrobenzene solution on the cap of a BLK agar plate. Comamonas was incubated at 30° C, and the liquid cultures were shaken at 250 rpm. The genomic DNA was obtained by using the Gram-positive bacteria purification protocol from the DNeasy® Blood & Tissue Isolation Kit (Qiagen) because Comamonas sp. Strain JS765 is classified as a gram-positive bacterium in fresh cultures. The kit included a lysis buffer and proteinase K for cell lysis and protein degradation respectively, and it utilized a DNeasy Mini spin column and collection tubes for washing and collecting the desired DNA. Each sample was collected by eluting the DNA with buffer twice into the same collection tube to ensure maximal yield. Elution of the sample was then verified by gel electrophoresis using a 1% agarose gel with ethidium bromide as the staining agent. Gels were visualized using a UV broad-spectrum light and DNA concentrations were determined by UV-spectroscopy prior to loading the gels. Following genomic isolation, the Polymerase Chain Reaction (PCR) was used to amplify the desired α and β subunits of the NBDOS. PCR reactions contained1.1 μL of both forward and reverse primers (10 μM stock), 100 μg of sample DNA, 45 μL of Platinum PCR Supermix, and sufficient sterile deionzied water so that the total reaction volume was 55 μL. Forward and reverse primers for amplifying the nbzAc and nbzAd genes were designed according to the following criteria: each primer contains approximately 20 nt that are complimentary to the desired sections of the NDBO genes, their overall length was minimal to reduce the chances of binding to themselves but sufficient to be specific to only our area of interest, and that their Tm values were within 5°C of each other. As for the actual primers designed, the forward primer was a 38 bp long strand including the Sgf I restriction site with a total melt temperature of 64°C and a melt temperature of 53°C for the homologous region, and the reverse primer was a 33 bp long strand including the Pme I restriction site with a total melt temperature of 63°C and a melt temperature of 54°C for the homologous region. For the specific primer sequence see Table 3. The reaction conditions were then set to a hot start of 3.5 minutes at 94°C, 5 Table 3: Information on the forward and reverse primers used for PCR amplification and engineering of the Sgf I and Pme I restriction sites onto the NBDO genes. Length (bp) Full Melt Temp Homologous Region Melt Temp Forward Primer 38 64°C 53°C Reverse Primer 33 63°C 54°C Forward Primer Sequence 5'- TTA TGC GAT CGC CAT GAG TTA CCA AAA CTT AGT GAG TG – 3' Reverse Primer Sequence 5 CGT AGT TTA AAC CAG GAA GAC CAA CAG GTT GTG 3 Table 4: Color code guide for the primer sequence in Table 3. cycles of: 94°C for 30 seconds, 48°C for 30 seconds, and 72°C of 2 minutes, then 25 cycles of 94°C for 30 seconds, 54°C for 30 seconds, and 72°C or 2 minutes, and final cool period of 72°C for 10 minutes and 4°C until removed from the thermocycler. The PCR product was then analyzed by gel electrophoresis. The PCR product was purified using the Promega Flexi® Vector Systems Kit, and then digested using the same kit. This kit utilized three key features; the rare restriction sites of Pme I and Sgf I, the lethal barnase gene, and the kanamycin resistance gene.11 The restriction cite tagging was done by engineering one PCR primer with the reverse primer with the Pme I sequence and the forward primer with the Sgf I. The presence of a the lethal barnase gene was essential because not all of the plasmids had inserted genomic DNA, so this barnase gene was located between the two restriction cites and if one of these plasmids made it to a growth plate they would naturally die off. Finally, the kanamycin gene was utilized once the plasmids were inserted into the cells, because if a cell took up one of the transformed plasmids they were able to survive on kanamycin laced growth media. The digestion process then required the purified PCR product and the Promega Flexi® vector to be digested separately using 4 μL of a 5x Color What it is Yellow Start codon of alpha subunit (2528 -30 bp) Green SgfI restriction sequence Dark Blue 4 extra bp Purple Codon before/upstream of stop codon for the beta subunit (4465-67 bp) Light Blue PmeI restriction sequence Red 4 extra bpFlexi® Digest Buffer, 2 μL of the Flexi® enzyme blend (Sgf I and Pme I) and up to 200 ng of the purified PCR sample and the Promega Flexi® vector. The vector chosen for the transformations of both E. coli strands was the pF1K T7 Flexi® Vector (seen in Figure 5). This vector was chosen in combination with E. coli (New England Biolabs, Inc. Cat. Figure 5: The restriction map of the pF1K T7 Flexi® Vector.10 The Sgf 1 is located at100 bp, Pme 1 is located at 499, the T7 promoter is located at 21 bp and the lacO is located at 39. No. C2987I) NEB 5α because it is a commonly used cell line for subcloning due to its lack of nucleases commonly found in other strains that would destroy the recombinant DNA the project is trying to generate, and E. coli BL21 (DE3) was chosen because it is able to produce the T7 RNA polymerase necessary for transcribing the NBDO gene located in the plasmid vector for later expression studies. The T7 RNA polymerase is a viral polymerase responsible for transcribing the inserted α and β NBDO genes, which will be inserted immediately downstream from a T7 promoter on the vector, and it is not found naturally in any prokaryotic cell lines.9 What this means is that E. coli BL21 (DE3) is a line of E. coli that was previously genetically engineered to incorporate the gene responsible for the production of the T7 RNA polymerase. Also, this means that along with the α and β NBDO genes being present on the vector there must be a T7 promoter and transcription regulatory genes. Gene expression is regulated in the T7 Flexi® Vector by the lac operon. When the lactose analog IPTG is present, the lac operator is not repressed and transcription of the gene proceeds rapidly due to the abilities and tendencies of the viral T7 promoter and T7 RNA polymerase.9 Since the maximum volume of the digest blend was 20 μL and the PCR product was not of high enough concentration to max out the 500 ng limit of the digest, all samples ran used a full 12 μL of purified PCR product to maximize the plasmid concentration (12 μL was the maximum volume aloud for in the reaction after the volumes required for the buffer and restriction enzymes were added). The digestion required a 30 minute incubation in a 37°C water bath for both samples and a second incubation of the vector in a 65°C water bath for 20 minutes. The PCR product and digested vector were then purified separately using a Wizard® SV Minicolumn and multiple rounds of washing by the according to the standard protocol described in the Promega Flexi® Vector technical manual. Finally, the purified, digested PCR product was ligated to the purified, digested vector. The ligation reaction consisted of 10 μL of 2x Flexi® Vector Buffer, 5 μL of digested vector, 4 μL of digested PCR product, and 1 μL of T4 DNA ligase followed by a hour long incubation at room temperature. Competent E. coli (New England Biolabs, Inc. Cat. No. C2987I) NEB 5α cells were transformed with the products of the ligation reaction according to the High Efficiency Transformation protocol from New England Biolabs (protocol CC2987). In this transformation procedure, 50 μL of cells were mixed with 5 μL (up to 100μg) of the ligated plasmid. The mixture was incubated for 30 minutes in an ice-water bath, 30 seconds at 42°C and then again on ice for 5 minutes. 950 μL of LB broth was then added and the mixture was incubated at 37°C for 1 hour in a shaker at 250 rpm. Cells were then plated onto Difco Nutrient agar plates containing the antibiotic kanamycin (50 μg/mL) to inhibit the growth of any E. coli cells that were not successfully transformed with the plasmid. Plasmids from kanamycin-resistant transformants were isolated using the Quantum Prep Plasmid Mini Prep Kit (BioRad). Cells were grown in LB broth containing kanamycin (50 μg/mL) and harvested by centrifugation at 14,000xg. The cells were resuspended in the Cell Resuspension solution, then lysed using a lysis solution, and a Neutralization Solution to denature any proteins and stabilize the plasmid. The sample was then washed with buffer and collected by centrifugation and loaded onto a Wizard® SV Minicolumn. The sample bound to the column was washed with wash buffer and then eluted out in H2O. The identification of proper transformation and isolation was then verified by gel electrophoresis of the product. Following the isolation of the plasmid, the plasmid was transformed into E. coli BL21 (DE3). The transformation reaction contained 50 μL of cells and 100 ng of plasmid DNA. Incubations of 30 minutes on ice, a 10 second heat shock at 42°C, and then 5 minutes on ice again. 950 μL of SOC was then added and the solution was mixed at 37°C for 1 hour in a shaker at 250 rpm. After this incubation, the cells were plated out onto LB plates containing kanamycin and incubated overnight at 37°C. Results: The Comamonas sp. strain JS765 bacteria was found to grow best in a solution of BLK with a concentration of nitrobenzene at 0.750 mM. A series of tests were done in effort to find the most effective growing conditions, and the results of these tests can be seen in the Table 1. Table 1 Nitrobenzene (mM) : Nitrobenzene concentration dependence of Comamonas growth measured by UV-vis spectroscopy. Abs (600 nm) 0 0.26 0.250 0.44 0.500 0.67 0.750 0.80 1.000 0.67 1.250 0.59 1.500 0.21 The integrity of the genomic DNA isolated from Comamonas sp. strain JS765 using the Qiagen DNeasy® Blood and Tissue Isolation Kit was verified by agarose gel electrophoresis. The concentration of the genomic DNA was measured by UV-visible spectrometry. The spectrophotometric results showed a range on concentrations from 16.59 ng/μL to 45 ng/μL, and the results can be seen in Table 2. The band located in Table 2 Abs (260 nm) : Absorbencies of collected genomic DNA of Comanomas sp. Strain JS765 as measured by UV spectroscopy. Abs (280 nm) ng/μLLane 4 of the gel indicates the genomic DNA due to its incredibly large size estimated at approximately 5 Mbp and can be see on Figure 6. Figure 6: Gel of genomic DNA following the DNeasy Blood & Tissue Kit After the genome was isolated, PCR was carried out under the conditions defined in the Materials and Methods section. The products of the PCR were analyzed by gel electrophoresis. The expected size of the PCR amplified product was a 1965 bp strand. However, as seen in Figure 7, the resulting strand was 2320 bp. This difference 0.3318 0.1652 16.59 0.4441 0.2122 22.21 0.6050 0.3125 30.25 0.3994 0.1732 19.97 0.90 0.55 45. 0.48 0.24 24.Figure 7: Gel of the amplified PCR product. in size was deemed to be an acceptable deviation from the expected size, and the project was continued using these strands. Following the PCR amplification, the product was digested using Sgf I and Pme I and ligated to the pF1K T7 Flexi Vector as described in the Materials and Methods section. This ligated product was then analyzed by gel electrophoresis, as seen by Figure 8. The expected size of the ligated plasmid was a circular plasmid of 5069 bp, which is the sum of the 1965 bp PCR product and the 3104 bp digested vector. Figure 8 shows that, when the plasmid construct was digested with Eco RI, a single band was observed at 6699 bp. And as seen in Figure 8, there is no such band at 5069 bp. However, the gel Figure 8: Gel of the ligated vector/NBDO gene plasmid. Lanes 1 and 4 are uncut plasmids, while lanes 3 and 5 are samples of the plasmid cut with EcoR1 and are thus linear. does suggest successful ligation because lanes 1 and Lane 4 contains the undigested circular ligated plasmid. Since the multiple bands seen in lane 4 all collapsed into one band in Lanes 3, it appeared as though the desired plasmid construct had been produced. As with the PCR results, even though the resulting product was larger than expected, the study proceeded to the next step, namely transformation of E. coli NEB 5α. This decision was made because the product would be sequenced later on in the study, and this later sequencing data is ultimately what is needed for confirming or rejecting that the gene was successfully cloned. The transformation of the E. coli NEB 5α with the plasmid DNA construct generated kanamycin-resistant clones. Kanamycin resistance is conferred by the Flexi Vector. In addition, since transformants carrying the original pF1K T7 Flexi Vector are not viable due to the lethal barnase gene, presumably all of the transformants carry foreign DNA. Figure 9 shows a negative control, in which untransformed NEB-5α cells were plated on nutrient agar plates containing kanamycin. As seen there is no growth evident on the plate indicating that the kanamycin successfully kills all bacteria that do not contain the kanamycin gene, such as the one found in the vector. In contrast, Figure 10 shows the results of the transformation experiment. Colonies indicate successful transformation because it must contain the kanamycin resistance gene found in the inserted plasmid. Also, it suggests that the plasmid contains the NBDO genes because the barnase gene, which would kill the bacteria, is absent from the inserted plasmid because the cells were able to grow and form colonies. Figure 9: Picture of the negative control. Figure 10: Picture of the transformed E. coli NEB 5α cells. Sequencing was performed on the plasmids collected from three colonies of the transformed E. coli NEB 5α cells (Northwoods DNA Inc, Bemidji, MN). The resulting sequencing indicated that the NBDO gene was contained within the plasmid of more than one of the clones. Two of the clones showed improper copying of additional sequences, however, one clone showed proper sequencing except for a single amino acid, which is not found in or near the active site. So from these sequencing results, it was concluded that the NBDO was properly ligated into the vector, and that it was done so with the proper sequence. After verifying that the ligated sequence was correct, the plasmid was isolated from the E. coli NEB 5α cell line and transformed into the E. coli BL21 (DE3) cell line. Again, Difco nutrient agar was used with kanamycin added. And as seen in Figure 11, transformants were successfully grown. Figure 11: Picture of the transformed E. coli BL 21 (DE3) cells. Conclusion The overarching goal of this study is to determine the reaction mechanism of the Rieske dioxygenase enzyme found in the bacteria Comamonas sp. strain JS 765, and while this was not determined in this specific project, the first step necessary for this goal was successfully performed. As seen by the sequencing results and the successful growth of both the transformed E. coli (New England Biolabs, Inc. Cat. No. C2987I) NEB 5α and the E. coli BL21 (DE3) cell lines on kanamycin containing Difco Nutrient agar plates, it can said that the desired α and β subunits of the NBDOS were successfully cloned and are suitable for further use in the amplification and over production of the desired native Rieske enzyme and of the mutated Rieske enzyme. It is important to note that while the gene was cloned into these cell lines, it may be necessary to use a mutagenesis kit to change the cell line containing the single-nucleotide polymorphism(SNP) back to the original nucleotide because this SNP changed the nucleotide from an adenosine to a guanine. Ultimately, it was not determined in this project if the change in amino acid substantially effected the function of the enzyme even though it was determined to be located away from the active site of the enzyme, but because the sequencing error was only a single change in nucleotide the cloning was determined to be a success and moving on towards mutating the enzyme for further study is going to be the next step for the project as a whole. What this next step in the project would include after the mutation would be growing both the native protein and the mutated protein and comparing the rate at which O2 is removed from the environment. This rate of O2 loss would indicate the rate at which the enzyme was working because the enzyme uses atmospheric O2 for the oxygen involved in the cis-dihydroxylation. And if it is found that the mutant is cable of utilizing the atmospheric O2 and its enzymatic rate is slower than that of the native protein, then it may be possible to capture any intermediate reactants, possibly the postulated Fe(V) intermediate. So while this study was unable to reach the point of studying the actual enzymatic mechanisms of the enzyme at hand, it was able to accomplish the first step of the process by successfully cloning the NBDO genes into both the E. coli (New England Biolabs, Inc. Cat. No. C2987I) NEB 5α and the E. coli BL21 (DE3) cell lines. Cloning the genes into these two cell lines accomplished two major advantages. The first being it is much easier to grow mass quantities of E. coli than Comamonas. The second being the E. coli BL21 (DE3) cell line is a line of E. coli designed for expressing proteins in higher than normal amounts, so when it is necessary for over-expressing the enzyme of interest it will be possible to produce more protein per cell than if it were to be left in the initial Comamonas cell line. References 1Lessner, D. J.; Johnson, G. R. American Society for Microbiology. 2002, 68, 634-641. 2Gibson, D.T., Parales, R.E. Opinion in Biotech. 2000, 11, 236-243. 3Minke, J.; Neibergall, M. Senior Thesis. Bethel University: St. Paul, MN 55112. 4Wackett, L. P. Enzyme and Microbial Technology. 2002, 31, 577-587. 5Wolfe, M. D.; Parales, J. V.; Gibson, D. T.; Lipscomb, J. D. J. Biol. Chem. 2001, 276, 1945-1953. 6Lee, S.K.; Nesheim, J.C.; Lipscomb, J.D. J. Biol. Chem.1993, 268, 21569-21577. 7Sinnecker, S.; Svensen, N.; Barr, E.W.; Ye, S.; Bollinger, J.M.; Neese, F.; Krebs, C. J. Am. Chem. Soc.2007, 129, 6168-6179. 8Ju, K. Parales, R.E. App. and Env. Microbio. 2006, 72, 1817-1824. 9http://www.bio.davidson.edu/Courses/Molbio/MolStudents/spring2003/Causey/pET.html 10http://www.lablife.org/p?a=vdb_view&id=g2.jDDeCIxtXK1IkpcKY.uoLytykos- 11Promega Flexi® Vector Systems Technical Manual 12Parales, R. E.; Huang, R.; Yu, C. L.; Parales, J. V.; Lee, F. K. N.; Lessner, D. J.; Ivkovic-Jensen, M. M.; Liu, W.; Riemann, R.; Ramaswamy, R.; Gibson, D. T. Applied and Environmental Microbiology 2005, 71, 3806-3814. 13Bruhn, C.; Lenke, H.; Knackmuss, H. J. Applied and Environmental Microbiology 1987, 53, 208-210. 14Nishino, S. F.; Spain, J. C. Applied and Environmental Microbiology 1993, 59, 2520- 2525. 15Nishino, S. F.; Spain, J. C. Applied and Environmental Microbiology 1995, 61, 2308- 2313. 16Bruhn, C.; Lenke, H.; Knackmuss H.-J. Applied and Environmental Microbiology 1987, 53, 208-210.
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Oregon man thrashes local children in treehouse-building contest
Article Summary
Everyone loves a treehouse - they seem to inspire a universal feeling of childlike wonder, and done right they really tickle the old 'living in harmony with nature' glands too. We've covered some beauties over the years here at Gizmag, but this one has to be the grand-daddy of them all. The work of architect Robert Harvey Oshatz, the Wilkinson Residence makes use of a steeply sloped block to put the house's main level right up in the tree canopy. Stunning from every angle, it uses curves and waves to echo the owner's love of the natural landscape with a slightly musical theme.
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Offering a fast and easy way to tension and secure smaller ropes and accessory cord, the Nite Ize Figure 9 Carabiner eliminates the frustration of tying, untying and adjusting knots around camp and on the trail.
Click here to visit the official Nite Ize website. (Note: you will be leaving Backcountry Edge.)


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Photo by Mark Zuckerman / NATS INSIDER
Yunesky Maya makes his 2011 debut today against the Padres.
When we last saw Yunesky Maya in Washington, the results were pretty unspectacular. In five September starts, the right-hander went 0-3 with a 5.88 ERA and made it through the sixth inning only once. But after a strong showing in the Dominican Winter League and then another strong showing this spring, the 29-year-old Cuban seemed to suggest he has turned a corner.
We'll find out today whether that's true. Maya has been recalled from Class AAA Syracuse, where he went 1-4 with a 3.79 ERA in nine starts, to pitch this series finale against the Padres. Of course, the way the Nats' lineup is producing right now, even a brilliant performance from Maya may not be enough.
Couple of lineup changes from yesterday. Roger Bernadina is back in the leadoff spot and in center field. Wilson Ramos is back behind the plate. Alex Cora is at second base, with Danny Espinosa getting a day off. You'll also notice Jerry Hairston is at third base, even though he was suspended one game by MLB for his actions Friday night with umpire Ed Hickox. Hairston (who is celebrating his 35th birthday) has appealed the suspension, so he's allowed to keep playing until his appeal is heard.
Updates to come…
PADRES at NATIONALS
Where: Nationals Park
Gametime: 1:35 p.m.
TV: MASN, Ch. 50
Radio: WJFK (106.7 FM), WFED (1500 AM), XM 185
Weather: Partly cloudy, 85 degrees, Wind 10 mph out to LF
CF Roger Bernadina
SS Ian Desmond
RF Jayson Werth
LF Laynce Nix
- Stranded_in_Philly - May 29, 2011 at 4:18 PM
Nice matchup of AAA lineups today.
- Carl in 309 - May 29, 2011 at 4:36 PM
Here's hoping Maya takes advantage of his opportunity; and a great day for a little hitting break-out (why one would expect that is a different matter).I do sense some anxiety/edginess around the team, and among its fans, but I found the game on Friday evening (which I watched at the ballpark) was encouraging for the crispness of the play. Especially considering we have no Z-man at 3rd, and MM was fielding throws at 1st. Always preferring to see the glass half full (even when it's arguably barely a quarter filled), reading Mark Z.'s early note on Maya's call-up that the Nats are the last team to reach for a 6th starter was a part of what gives me greater satisfaction in watching this team–even at it's maddeningly most troubling (e.g., blowing a big lead when on the road recently). Lastly, a couple of readers wondered if the Nats are being "squeezed" by the umpiring crews and disrespected by the MLB front office (I suspect they are) in commenting on the 1-game suspension for Jerry Hairston. Much as I admire the work of umpires in general, I am not dazzled by consistency or fairness. But Hairston was aggressive–that was the whole point of his tirade. Like Riggleman's on Bernadina's bunt hit/out.
- Anonymous - May 29, 2011 at 4:40 PM
"Its Riggleman more than Rizzo for the 25-man. Rizzo, Clark, Kline, etc. more than Riggleman for the 40-man and down into the minors."How do you know?What are your sources?Boz, (Thomas Boswell) for the 450th time, who has more insider connections to the Nationals and Orioles than probably any other beat writer period. If you are tired of broken records anon then stop asking. Need additional references?Here:Ben Goessling said: I think Boz has a keen sense of what's going on with the team. STOP ASKING and I'll stop beating you over the head with Boz's quote. Which inexorably proves my point, like it or not.
- Anonymous - May 29, 2011 at 4:44 PM
And just to be clear Boz doesn't blame Rizzo or Riggleman for the roster. He points upward … as he said, one major league executive was a bit miffed that the Nats salary level has remained the same for over 5 years. Under their market level. It is my contention that Werth was also pointing upward. Not entirely but mostly in that direction. Ted Lerner was regaling players with how much he wanted to win for old DC during spring training. Time for the rubber to meet the road. Way past time.
- slade - May 29, 2011 at 4:52 PM
Hard to believe we did not have a better option than Maya….disappointing
- Neal - May 29, 2011 at 5:04 PM
I'd have set them up this way: Bernie, Hairston, Nix, Morse, Werth, Ramos, Cora, Desi and Maya……but that's just me.
- phil dunn - May 29, 2011 at 5:07 PM
I was going to the game today but cancelled my plans as soon as I saw that Maya was pitching. Only the Nats would promote a pitcher who was 1-4 at Syracuse. That speaks volumes about the quality of their minor league pitching prospects. Just about every highly touted minor league pitcher has turned out to be a complete dud. By the way, anyone know how much money Rizzo blew on Maya? I seem to recall it was a fairly significant amount.
- Anonymous - May 29, 2011 at 5:13 PM
prediction–Moseley shuts out Nats on two hits and Riggleman and Eckstein are fired after the game. Rizzo has to know by now that Werth won't stop pouting until Riggleman is gone. Big shake up is needed!!
- Anonymous - May 29, 2011 at 5:33 PM
Nothing like rooting against the home team and campaigning for people to be fired. What a nice way to start the Memorial Day weekend.
- Anonymous - May 29, 2011 at 6:01 PM
So much for the predicted shutout.
- Anonymous8 - May 29, 2011 at 6:04 PM
Mark Zuckerman writes…Some serious "SmartBall" (TM) in the bottom of the first gives the Nationals a 2-0 lead. Roger Bernadina reached on an error by first baseman Brad Hawpe, then stole second. Ian Desmond beat out a perfect bunt down the third-base line. Jayson Werth then perfectly placed a hit-and-run single past the second baseman's position to score the day's first run and advance Desmond to third.It has been weeks since we have seen "small ball" aka "smart ball" which is what won so many games early in the season. It demoralizes the opponents and pumps up the Nats dugout.Certainly "big inning" baseball is great for good offensive teams which the Nats clearly aren't right now.
- Anonymous8 - May 29, 2011 at 6:07 PM
If Hairston gets a "hit" on his bouncer to 3rd, how doesn't Bernadina get a "hit" on his smash to first in the 1st?
- Anonymous - May 29, 2011 at 6:49 PM
Forget the shutout. This might prove something I've been thinking … once they promote Brad Peacock the Syracuse Chiefs might just have a better starting rotation than the Washington Nationals. That's Rizzo's strength, the big club might look pathetic but the minors are dealing compared with just 2 years ago.Its good news. Pitching bright enough to require sun glasses.If you're looking for offensive help, that's likely there too but its likely a ways off … around 2+ years I'd imagine.
- JaneB - May 29, 2011 at 7:16 PM
Tell me why why why they bring in Slaten when he is so bad with inherited runners? Why?
- sm13 - May 29, 2011 at 7:20 PM
Gee, Maya's act looks a bit familiar — 3 or so decent innings and then he blows up and can't make it past the 5th. Doesn't seem like his time in AAA has changed a thing. I hope we give someone else — Craig Stammen, hopefully — a chance to fill in for Gorzo in Arizona.
- JaneB - May 29, 2011 at 7:30 PM
What sm13 said. We've seen enough.
- Big Cat - May 29, 2011 at 7:36 PM
Maya was promoted because of the money we blew on him. Next to come up will be Detwiler, because he was a #1 draft pick. He should still be in Potomac. Check his stats down there. He got hammered. He never earned a promotion, he was given one
- NattyNation - May 29, 2011 at 8:14 PM
WHHHHYYYY is berni swinging?? just do your job. that is right back on riggs. where is the discipline? what are we doing?
- NG - May 29, 2011 at 8:16 PM
Can MLB actually increase Hairston's suspension when they hear his appeal? Like, say, 110 games?
- Unkyd - May 29, 2011 at 8:21 PM
Ohhhhhh, Desi….. wait on it….. sigh…
- FS - May 29, 2011 at 8:33 PM
I do not trust our batters at all from now on except Zimm when he comes back. A runner at 2nd and I would not trust anyone to get him home. Whoever the batter, just straight up ask them to bunt the runner over to third.We can not really wait for a 3-run homer with these lineups.
- JaneB - May 29, 2011 at 8:35 PM
I'm turning into JayB instead of JaneB. They need to get rid of Stairs so theybRe never again tempted to pinch hit him again. And send down Slaten while they're at it. Shhesh. Am I grumpy!
- Manassas Nats Fan - May 29, 2011 at 9:08 PM
I have come to the conclusion that none of the front office, coaching staff or players studied much history.Winston Churchill siad, "He who doesn't study and learn from history is bound to repeat history."Well lets see Doug Slatin can't stop inherited runners from scoring, so h is brought in to pitch with men on and some score.The sacaraffice buntoday worked very well including 2 hits. So when Bernie fouls one off it is taken off.Storin gives up a run three straight games now.But worst of all the Hitting with RISP just terrible.A;sp when Hairston was picked off why he just stand there and be tagged. Take off for second, and maybe the runner can come home. What was that all about?Tomorrow is a new day, and I say beat the Phillies 2 out of 3. They need to pitch, hit, field and run the bases with no mistakes.
- Jeeves - May 29, 2011 at 9:10 PM
Oh boy, another first round pick coming up. Good grief, I've become a negative poster. There is no hope. Heck even Jane B has shown tendencies in the same direction. If Natsfan1 goes there, well…
- FS - May 29, 2011 at 9:12 PM
Use of Stairs again and again is inexplicable. Does he have some material against Riggleman and Rizzo that he is threatening to make public on his unconditional release? Slaten has been sucking since day one too.Rizzo needs to admit his mistakes and attempt to correct them now.Also the talk of 'this-team-being-better-than-standings-indicate' does not seem to hold any truth to it anymore. Very frustrated with this team.
- Anonymous - May 29, 2011 at 9:28 PM
The Nats better keep giving away "baby bats" because, at the rate they are playing, there won't be 5,000 fans in the stands without a giveaway. Word Up!
- Eugene in Oregon - May 29, 2011 at 9:38 PM
Ugly, ugly, ugly. I was considering a quick trip down to SF to see the series with the Giants, but I just don't think it would be worth it…Mark – One request – when next you have an opportunity to ask Rizzo or Riggleman, please get an on-the-record statement as to why they are keeping Stairs around.
- Anonymous - May 29, 2011 at 9:54 PM
Hey Eugene. Go down to SF! The Nats will need a friendly face in the crowd and you will probably get on TV here (wear your Nats RED!). And, hey, are you Eugene from Eugene? My bro lives in Corvallis! Go Beavers!
- Get Some Players - May 29, 2011 at 10:00 PM
Josh Willingham just hit a 3r homer off the O's best pitcher (Britton). The Lerners apparently couldn't afford him. Nice job Rizzo/Lerner.
- Anonymous - May 29, 2011 at 10:10 PM
One thing is for sure … if you want to return Maya to AAA you had better send Desmond along with him. And Stairs and Cora were signed to minor league contracts … its a shame they can't be assigned there as well.
- Grandstander - May 29, 2011 at 10:13 PM
So, considering the type of baseball this team is playing, how many of you brave souls are going to trek to Citizens Bank Park South tomorrow?I know I'm not going to ruin my Memorial Day.I will lay off my Matt Stairs hate because that ball was actually hit pretty well. But Hairston… My oh my, Jerry, I hope you get an angry call from your father tonight. That was some serious ugly baseball today.Mark, I don't even think you need to write different headlines for your gamers anymore. Just keep using squandered opportunities. It seems to hold true every day.
- Anonymous - May 29, 2011 at 10:13 PM
With 3 games against the Phillies and the Nats looking like a good way to up their win totals … things could get decidedly worst.Small ball is not "smart ball" Giggleman, its losing ball
- Anonymous - May 29, 2011 at 10:21 PM
Get to say it twice to correct my error ;)With 3 games against the Phillies and the Nats looking like a good way to up their win totals … things could get decidedly worse.Small ball is not "smart ball" Giggleman, its losing ball!
- Rabbit - May 29, 2011 at 11:32 PM
@Grandstander…….It looks like my day will be ruined. I'm going to the game because the tickets were a gift. Obviously from people who hate me! At least I'll see the second best team is baseball. Halladay will have a great chance at another no-hitter. I figure we will need at least seven runs to win against the Phillies. A good bet would be that Phillies will score more runs than we get hits. Oh well, at least when I get home I can jump in the pool.
- Manassas Nats Fan - May 30, 2011 at 3:23 AM
I feel a win coming tomorrow (heck I feel that every day though)
- Anonymous - May 30, 2011 at 10:44 AM
Milone or Meyers deserved the callup more than Maya. But at Lerner Field, getting top prospects up before the Super 2 deadline isn't likely to happen, even if costs the team some games.


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Audrina Patridge Biography
Audrina Patridge stars on The Hills. Maybe you recognize Audrina Patridge from that show ... or from those hot nude pics that surfaced...
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- Born
- Birthplace
- Yorba Linda, California
- Full Name
- Audrina Cathleen Patridge
Audrina Patridge Quotes
[The role is] giving me the chance to show people [what I can do]. This is why I moved to L.A.
Audrina Patridge [on snagging a movie role]
- Permalink: giving me the chance to show people . This is why I moved to L...
- Added:
Lauren Conrad [on a random Les Deux club-goer]: He thinks I like him.
Audrina Patridge: He thinks you like him?
Lauren Conrad: Seriously, I never wanted to stab myself in the eye more.
- Permalink: He thinks I like him. He thinks you like him? Seriously, I ...
- Added:


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I am just tickled with how beautiful this skin looks on my galaxy note. It was quite easy to put on and no doubt from the looks of it will be very durable. I will be ordering one in a photo of my new grand child.. What a great way for grandma to show him off!! Wonderful product and you can count on me being back. Not only that, but it arrived ahead of schedule and when sending things to Alaska that's just short of a miracle !bonnie Caarol, United States


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Special tax district OK'd for County Fair Food Store
The Dell Rapids City Council approved a special tax district for County Fair Food Stores at its March 18 meeting, paving the way for a new store at the corner of 10th Street and Highway 77.County Fair's proposal for a tax increment finance district includes diverting about $689,000 of property taxes from the new store and the development of the nine-acres of property toward the cost of infrastructure improvements in the area.Site preparations include installing retaining ponds, water and sewer, lot entrances and sidewalks surrounding the property as well as grading work.An additional $250,000 was included in the plan to allow the city to upgrade its drainage system along the east side of Highway 77 from 10th Street to Eighth Street.The proposal had unanimous support from the council.John Clarke, owner of County Fair Food Stores, said he's pleased with the council's support of the project and hopes to get started with construction by late spring or early summer, with hopes of having the new $2.88 million, 30,000 square foot store open and operating by year's end. Throughout the TIF proposal process, which received approval from the planning and zoning board March 12, Clarke has made the case that establishing the TIF district is in the city's long-term interest.Clarke told the council the new, bigger store will add 15 to 20 more jobs to his Dell Rapids payroll as well as a wider variety of services and products.On top of more jobs at his store, Clarke said the city would see a significant increase in sales tax and liquor sales tax revenue, estimated to be as much as $59,500 each year above what is garnered from store sales now.Clarke expects the city to also see one-time revenue increases from equipment purchases during the construction of the project, which he estimates to be around $36,000.Clarke's plans also call for a 3,500-square-foot out lot building and a 20,000 square-foot retail office on the property to come a few years later.In all, Clarke expects about $5.3 million worth of construction on the lot.
Argus Leader
Dell Rapids Public School
Dell Rapids St. Mary's Catholic School
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I definatly spured more comments then a lot of othere (and serious) news have
For those that haven't noticed, all Phoronix news is not written very seriously... if there's a Linux event/news that's also funny, entertaining, weird, or interesting, it also sometimes gets mentioned - especially if it's going to lead into an interesting forum discussion. Note that this article is titled "A New Open-Source License..." and begins with "Some user, after having..." I certainly don't think Maximal will be overtaking the GPL anytime soon
Michael Larabel
I definatly spured more comments then a lot of othere (and serious) news have
Sounds like a product of, or inspired by the Linux Action Show.
"Negative in the freedom dimension" started it off.
Defiantly not, I'm a bit dyslexic though.
What a great idea to come up with a new license. I can do it too!
DtRT License.
This source code is released under the DtRT license. To comply with this license, you must Do the Right Thing with the source code.
If there is anything but querulants here, have a look at my thinking here, where I actually detail some of the happenings around this. I wrote this licence after realizing RMS associated idolaterous religion, and unreasonable thinking (open source is not open source etc) with this.
Please also see my standard reply to retards at the end.
Well, the legal issue is a bit of a catch 22. Because if something ends up in court, it basically comes down to "But these are the terms which you have read and agreed to."
But now, we have the situation that for a lisence agreement to stand up in court it has to be so complicated that no one can understand the agreement that "they agreed too". But, if you make it simpler and shorter, like this lisense, people might actually read AND understand it. But, then it won't hold up in court.
Personally, I think that it should become an international law, that each and every single lisense agreement has a short layman's explanation at the top of what they're basically agreeing too. That way, ordinary users know what they are agreeing too, and if it does turn into a law-suit the laywers can throw the specific detailed clauses at each other to their heart's content.
What should be so difficult to understand about open vs closed source? What country is this?


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Mammoth's new interim town manager has agreed in principle to a $158,000 annual salary, according to town documents.
Daniel C. Holler, who is to begin work on Tuesday, Oct. 1, is expected to agree to the terms one day later when the Town Council approves the contract at its regular meeting.
Holler, 52, arrives from Grass Valley, where he was city administrator.
Holler's contract is $25,000 a year less than the salary given to outgoing Town Manager Marianna Marysheva-Martinez. That works out to be about 24 percent less.
For the first time ever, the Town of Mammoth Lakes has entered into negotiations regarding the possible purchase of a portion of Sam's Woodsite.
The property, situated at 5699 Minaret Road, between Meridian Boulevard and Main Street, is the site of some of Mammoth's most popular summer events, including the wildly successful annual Festival of Beers and Bluesapalooza.
The whole Woodsite totals eight acres of woodland that borders on the Sierra Star Golf Course.
The strength of any community during a disaster is in its people, a fact recognized by professional first responders across the country who rely on teams of trained community members for everything from disaster field reports to traffic control.
Mammoth Lakes is no different and beginning next week, a new round of Community Emergency Response Team (CERT) training kicks off, with an introduction to the training on Thursday, Oct. 3 at the Best Western Conference Room from 6-7:30 p.m.
Three all-day Saturday sessions will follow, beginning on Oct. 19.
In a move that ought to make local food growers egg-static, Mammoth is forging ahead with creating a policy for raising and keeping chickens.
"The existing zoning code provides no guidance with regard to the raising of chickens for egg-laying purposes within the town limits," wrote associate planner Jessica Morriss of the town staff.
The first dusting of snow for the Eastern Sierra came right on schedule last weekend, heralding the arrival of the Autumnal Equinox.
Mammoth Mountain Ski Area took on a few inches of snow, golden aspens turned a deeper gold, flowers shriveled on their stalks and gardeners across the county rushed to cover their cucumbers and late-ripening squash as temperatures dipped into the mid-twenties by Thursday morning.
It is, however, probably a good idea not to get too excited about it all.
The new Mammoth Lakes interim town manager is Dan Holler, formerly city administrator for Grass Valley, Calif., a Gold Country Sierra community near Nevada City.
In light of Marianna Marysheva-Martinez's resignation this past week and the rumors leading up to her decision, we've witnessed the reactions of our passionate Mammoth citizens, not just to the fact of her resignation, but also to how the whole thing happened.
To put it bluntly, the reactions tell us, not very well.
Momentum is not something for which anyone can plan. It's either there, or running backward.
Right now, Mammoth has lost its "Big Mo," and is settling into an uncomfortable ennui.
We hope it doesn't last long, but the signs are not good.
This was supposed to be the summer that was going to build on last year's "Best Summer Ever," but it did not happen quite the way anyone could have predicted.
For the second straight weekend, the California High Patrol this past week used aircraft to nab speeders on U.S. 395, this time in a stretch between Lee Vining and June Lake.
According to a news release issued on Monday, Sept. 23, the CHP said it busted 13 speeders and made 16 stops in a 2-hour, 30-minute air operation the previous day.
Officers and pilots in the air coordinated with officers and units on the ground, according to spokesperson Officer Anne Morin, the public information officer of the California Highway Patrol, Bridgeport Area.
To hear coach John Morris tell it, the Mammoth Huskies volleyball team is fired up and ready to go.
Following a third-place finish last weekendŌĆÖs Cal City Tournament, the Huskies begin High Desert League play on Friday, Sept. 27 against Desert at 4 p.m. and Desert Christian on Saturday at noon.
Both matches will be in the MHS gym.
The team, Morris said, is on a nice roll, following its fourth-place at the Mammoth Invitational on Sept. 13-14 with a third-place finish at Cal City on Sept. 20-21.
The Mammoth Lakes Chamber of Commerce has taken a firm stand on raising the minimum wage by $2 over the next two years.
It doesnŌĆÖt like it.
In a strongly worded statement that went to its members on Monday, Sept. 23, the Mammoth Chamber urged opposition to a law that, if signed into law by Gov. Jerry Brown, would raise the minimum wage from $8 an hour to $10 an hour over the next 18 months.
ŌĆ£The Mammoth Lakes Chamber of Commerce supports a fair wage in California and supports incremental increases related to the Consumer Price Index (CPI),ŌĆØ the Chamber said in a news release.
The Mammoth Huskies learned a thing or two about the art of a quick turnaround Friday night against the Desert Scorpions at Edwards Air Force Base.
Cruising along comfortably after two quarters and with a possible road victory within sight, the Huskies came out of the locker room at halftime and were stung so quickly it was hard for them to know what bit them.
The final was 55-27, but in the third quarter, Desert overwhelmed Mammoth repeatedly, putting up 30 points in less than 12 minutes and putting the game out of reach.
It was a hot, still morning and the smell of summer was adrift on the hazy golden air—pine sap; sage; the deep green scents of manzanita and ceanothus; the lavender aroma of lupine gone to seed.
Even the bees, spiraling through the waning fireweed along the trail, seemed lazy: somnolent, barely able to keep their heavy, furred bodies alight.
When the hot, dry summer winds that sweep through the Mono Basin with great regularity encounter a spark of fire, a wildfire can spread across dozens of acres of sagebrush and bitterbrush in a few minutes.
That's exactly what Mono City residents have worried about for years, a worry made more pronounced by the fact that there is only one good road going in and out of the community.
Mammoth's mountain bikers, fresh on the heels of a long season, already are preparing for 2014.
On Thursday, Sept. 26 at 6 p.m. at Giovanni's, Fat Bike Mammoth will conduct a meeting to discuss mountain biking opportunities in Mono and Inyo Counties.
Specifically, according to Fat Bike leader Alan Jacoby, the group will discuss "becoming a more formal group" and working with the Forest Service and Bureau of Land Management "to increase and improve our opportunities.


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Lite Source Parvati Wide 20" H Table Lamp with Drum Shade
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24 Issues of Better Homes and Gardens
Included with purchase ($10 value).
Lite Source Parvati Wide 20" H Table Lamp with Drum Shade
Weights & Dimensions
Product Type: Standard | Style: Modern |
Fixture Material: Metal | Number of Lights: 1 |
Bulb Type: Fluorescent | Light Direction: Multiple Directions |
Wattage per Bulb: 60 Watts (W) | Bulbs Included: No |
Shade Included: Yes
| Built-in Outlet: No |
Adjustable Height: No | Night Light: No |
Swing Arm: No | Switch Type: On/Off Switch |
Lifestage: Adult | Gender: Unisex |
Commercial Use: Yes | Eco-Friendly: No |
Product Care: Do not use strong liquid cleaners | Country of Manufacture: China |
Assembly Required: Yes
| Additional Parts Required: No |
Product Warranty: 1 year |
cUL Listed: Yes | UL Listed: Yes |
Energy Star Compliant: No | Title 24 Compliant: Yes |
About the Manufacturer
To put it plainly and simply, Lite Source is a quality manufacturer of a vast selection of both beautiful and affordable interior lamps, not to mention a small number of other household items. From unique floor lamps and contemporary table lamps, to standard desk lamps, any Lite Source purchase will be a long-lasting addition to your existing décor, and a highly functional source of light!Lite Source manufactures a beautiful selection of high quality accent lamps, ceiling lighting, wall lighting, exterior lighting and home accessories. From novelty lamps in unique styles to standard desk and floor lamps, a purchase from Lite Source will be a long-lasting addition to any decor and for any application.
Lite Source Parvati Wide 20" H Table Lamp with Drum Shade
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Tell us what you think about this product. It will help us provide you with better products in the future and help your fellow shoppers make better purchases.
Write the first review Reference
Parvati Wide 20" H Table Lamp with Drum Shade is also sometimes listed under the following manufacturer numbers: LS-21270AB/AMB


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Wreaths, Inspirational Gifts, Fine Art, Figurines, Home Décor and more
Our 2015 Spring Wreaths are now available! Offering a wide variety of high quality home decor products from Darby Creek Trading including floral wreaths, inspirational gifts , decorative wooden ducks, figurines, and outdoor art. Leading our line are our made-to-order wreaths including outdoor wreaths, indoor wreaths, dried wreaths, preserved wreaths, silk wreaths , silk flower arrangements, and tabletop floral. Our selection of wreaths is the largest dried, preserved, and artificial wreath selection available. Each season we introduce a new collection of Christmas wreaths , spring wreaths , heart wreaths , and fall wreaths . We continue to expand our line of inspirational prayer plaques and angel figurines. In addition, we have many inspirational gift items including Irish blessings, Serenity Prayer plaques, and Footprints in the Sand poems. Our large selection of customizable memorial plaques and stones are always very popular. Be sure to inquire about our customized inspirational prayers and customized floral wreaths and arrangements.
Our weatherproof outdoor art prints as well as original oil canvas artwork tapestries are among the finest available. Don't forget to take a look at our hand carved wood ducks and other wood carvings. Our bronze figurines continue to grace the homes of many satisfied customers. With more than 2,000 home decor and gift items, we're sure you will find that special gift for a loved one or your home. Our decorative vases are high design art that can be used with floral arrangements or as a stand-alone piece of art. We are your home decor source for all of your accent pieces. Call about our custom inspirational & prayer plaques for a wedding or special spiritual occasion. We offer custom weather proof outdoor art for artists, interior designers, architects and photographers. With access to over 200,000 fine art prints we can find what you are looking for. Darby Creek has the largest selection of custom made dried and preserved wreaths available so you are certain to find that special wreath for your home. Be sure to inquire about customization of your inspirational prayers, memorial pieces, floral accents, and outdoor artwork. When it comes to home decor, Darby Creek's products are unsurpassed in quality and design. We carry the finest lines of home decor from the leading galleries like Genesis Fine Arts, Fine Art Tapestries, and Weatherprint Outdoor Art.


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All our Performance Mufflers are made from the highest quality polished stainless steel. Designed and constructed to maximize your vehicle's power with this straight through concept. Full T-304 Austenintic Stainless Steel Construction. Hi-Power Muffler incorporate a race inspired angled mounted stainless steel muffler and piping design.
*Welding is required for installation. Not BOLT-ON product.
Mufflers | |
Material | Stainless Steel |
Color/Finish | Stainless Steel/Black |
Inlet Size | 2.5 inches |
Tip Size | 4 inches |
Tip Finish Angle | Slant Cut, Burnt Look |
Removable Silencer | Included, Black |
Copyright © 2012 AIT Racing, All Rights Reserved.
All trademarks are the sole property of their respective companies. All prices and specifications are subject to change without notice.
AITRacing.com is not responsible for typographical errors. All typographical errors are subject to correction.


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This fun scrappy quilt is quick to put together and great for beginners. Use lots of different colors to keep it bright and fun…or just a few colors to keep it calm and simple.
Finished Size: 36" x 41"
1. You'll need to cut 27 color strips 2" x width of the fabric (at least 36") and
2 white strips 1 1/2" x width of the fabric (at least 42").
2. Lay the color strips out in the order you want them.
3. Using a 1/4" seam allowance, sew the strips into pairs first…then sew the pairs all together. Alternate the sewing direction when sewing the strips/pairs together, and don't pull the strips…this will help prevent stretching and keep the strips straight.
4. Press the seams open. This takes a bit more time but the nice straight seams are worth it!
5. Fold the length of the quilt top in half and align it carefully on the cutting mat. Trim the edges straight until the quilt top is 35" wide.
6. With the quilt still on the cutting table, make a cut 8" in from the right side. Make another cut 5" in from the last cut.
7. Sew a white strip onto each side of the 5" strip. Press towards the white strips and trim the ends.
8. Rotate the strip 180 degrees, and sew the strip back in between the larger pieces to make the quilt top. Press towards the white strips.
Fast, fun, and CUTE!


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