text:
PoliticsLithuanians vote out austerity government Government's efficient action of tackling financial crisis fails to win over voters weighed down by austerity measures. 15 Oct 2012 06:14 GMT | Politics, Europe, Greece, Ireland, Lithuania Voters usher in new government to be led by labour party 'Darbo Partija', Victor Uspaskich [GALLO/GETTY]Opposition populists and leftists in crisis-worn Lithuania have hinted they are prepared to form a government coalition after an exit poll late on Sunday indicated their parties would take first and second place in the country's parliamentary elections. The exit poll conducted by RAIT/BNS gave the biggest share of the vote, 19.8 per cent, to the Labour Party. And Labour's most likely coalition partner, the centre-left Social Democrats, were second with 17.8 per cent with the prime minister's Homeland Union in third place on 16.7 per cent. The ex-Soviet state, with a population of nealy three million, drastically suffered when the crisis hit four years ago. It was swift to slash spending in response and is now on course to return to economic health - but the belt-tightening failed to win over dispirited voters. Lithuanian voters threw out centre-right Prime Minister Andrius Kubilius, in favour of a coalition of left-leaning opposition parties who promise to alleviate austerity measures, according to the exit poll following Sunday's parliamentary election. The Baltic nations government failed to maintain its seats despite being applauded by big European powers and the International Monetary Fund (IMF) for its disciplined financial planning. Kestutis Girnius, from the Institute for International Relations and Political Science in Vilnius said: "If the IMF was voting then he (the prime minister) would be re-elected," adding, "But the IMF does not live in Lithuania, and they could not live on a Lithuanian salary." The country was one of the most severely hit European Union states by the financial crisis, and one of the quickest to react by instantly implementing austerity. It is a bellwether for the governments of Greece, Spain, Ireland and elsewhere, who are being forced to make similar stinging cuts.
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Amy Adams's Dress at SAG Awards 2014
Really, Does It Get Any Brighter Than Amy Adams?
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Amy Adams didn't borrow from her American Hustle alter ego for tonight's SAG Awards. Instead, it's more likely that the actress borrowed from Old Hollywood by turning out a glamazon look in bright cobalt Antonio Berardi. It's not just the color that wowed us: the asymmetrical cut of the dress showed just enough skin, while a jeweled belt, glittering Cartier earrings, and Salvatore Ferragamo clutch provided the perfect finish. Are you into it?
Vote on all of our SAG Awards polls here!
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Read More Red Carpet PollAward SeasonAntonio BerardiAmy AdamsFashion PollRed CarpetSAG AwardsCelebrity Style
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Benzara 31 in. Vase With Dark Brown Shade And Elegant Details
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Organizers of the Beaches International Jazz Festival say they are shocked after hearing they will receive no provincial funding this year. For the past few years, Ontario has given $75,000 to the annual event through the Celebrate Ontario program.
"I was shocked," executive producer Lido Chilelli tells Newstalk 1010, adding that he received the news in an email.
The funding represents about 20 per cent of the event's budget and without the funding, he says the festival will have to be scaled back this summer.
He says he still hopes the province will change its mind. NDP MPP Michael Prue is fighting for the funding to be renewed.
Prue says he acknowledges that other events compete for the funding as well, although it's not clear why the jazz festival lost out on the program this year.
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Page 1
Use of A U16 snoRNA-containing Ribozyme Library to Identify
Ribozyme Targets in HIV-1
Hoshang J Unwalla1, Haitang Li1, Shi-Yang Li1, Danny Abad1, and John J Rossi1
1 Division of Molecular Biology, Beckman Research Institute of The City of Hope, Duarte, California,
Hammerhead ribozymes have been shown to silence human immunodeficiency virus-1 (HIV-1) gene
expression by site-specific cleavage of viral mRNA. The two major factors that determine whether
ribozymes will be effective for post-transcriptional gene silencing are colocalization of the ribozyme
and the target RNAs, and the choice of an appropriate target site on the mRNA. An effective screening
strategy for potential targets on the viral genome is the use of ribozyme libraries in cell culture.
Capitalizing on previous findings that HIV-1 and ribozymes can be colocalized in the nucleolus, we
created a novel hammerhead ribozyme library by inserting hammerhead ribozymes with fully
randomized stems 1 and 2 into the body of the U16 small nucleolar RNA (snoRNA). Following three
rounds of cotransfection with an HIV-1 proviral DNA harboring the herpes simplex virus thymidine
kinase (HSV-TK) gene, we selected for gancyclovir-resistant cells and identified a ribozyme sequence
that could potentially target both the U5 and gag genes of HIV-1 regions on the HIV-1 genome
through partial homologies with these targets. When the ribozymes were converted to full
complementarity with the targets, they provided potent inhibition of HIV-1 replication in cell culture.
These results provide a novel approach for identifying ribozyme targets in HIV-1.
The nucleolus is a distinctive subnuclear compartment, thought of primarily as the site of
ribosomal RNA biogenesis and ribosome assembly. However, a number of studies have now
shown diverse roles for the nucleolus in mRNA production and even viral replication.1,2 For
example, several mRNAs have been shown to pass through the nucleolus (e.g., c-myc, N myc,
and myoD3) and at least one microRNA localizes to the nucleolus.4 Transcription and
replication of Borna disease virus (a negative strand RNA virus) occurs in the nucleolus5 while
human T-lymphotropic virus env RNAs6 and the human immunodeficiency virus-1 (HIV-1)
immediate-early proteins Tat and Rev have been shown to localize to the nucleolus.7–9 The
fact that these proteins interact with HIV-1 RNA, and the finding that a nucleolar-localized
hammerhead ribozyme provided strong inhibition of HIV-1 (ref. 10) constitute strong evidence
to suggest that HIV-1 RNA traffics through the nucleolus as part of its replication cycle. In
support of this notion, Cantó-Nogués et al. have demonstrated, using electron microscopy, that
HIV-1 transcripts can localize in the nucleolus and in peripheral dense chromatin in peripheral
blood mononuclear cells.11 We have previously shown that efficient inhibition of HIV-1 can
be achieved with nucleolar-localizing ribozymes and decoys for Tat and Rev.10,12,13
Ribozymes are small RNA molecules with distinct catalytic motifs that can be adapted for
trans-target cleavage by modifying the hybridizing arms to bind to target RNAs through
Correspondence: John J. Rossi, Division of Molecular Biology, Beckman Research Institute of The City of Hope, Duarte, California
91010, USA., firstname.lastname@example.com.
NIH Public Access
Author Manuscript
Mol Ther. Author manuscript; available in PMC 2009 November 10.
Published in final edited form as:
Mol Ther. 2008 June ; 16(6): 1113–1119. doi:10.1038/mt.2008.54.
NIH-PA Author Manuscript
NIH-PA Author Manuscript
NIH-PA Author Manuscript
Page 2
Watson–Crick base pairing. In the presence of magnesium, their intrinsic enzymatic activities
cleave the phosphodiester backbone of targeted mRNAs.14 Specifically, any RNA can be
cleaved as long as the ribozyme can pair with the target RNA and the target contains an NUC
triplet where N = A, G, C, or U. This property, combined with the ability of ribozymes to
undergo multiple turnover reactions, makes them attractive agents for modulating gene
expression.14 The two most common forms of ribozymes employed for mRNA cleavage are
the hairpin and the hammerhead motifs. Recently, a number of strategies involving libraries
of RNA-based antivirals have been employed in forward genetic screens for identifying genes
on the basis of their functions. For ribozymes, merely randomizing the hybridizing arms can
generate a library of 4n distinct ribozyme sequences, depending on the length of the hybridizing
arms (n). The application of ribozymes in forward genetic screens involves delivery of these
libraries to target cells, either through transient transfection or transduction, followed by
screening or selection of a particular phenotype. Using this approach, ribozymes that target
several important genes involved in viral and oncogenic pathways have been identified.15–21
Importantly, the library approach selects only for target inhibition, and does not require 100%
matched base pairing to the target.21 In general, therefore, this approach allows only the
identification of ribozymes that achieve the desired phenotype, and not necessarily of
ribozymes with the greatest cleavage activities.
We have taken advantage of a hammerhead ribozyme library approach to identify targets in
the HIV-1 genome that are highly susceptible to ribozyme-mediated downregulation. We have
also taken into consideration our previous finding that a nucleolar-localized ribozyme can
provide potent inhibition of HIV-1 replication in human T lymphocytes.10 The nucleolar
localization is accomplished by insertion of the hammerhead ribozyme into the structure of the
small nucleolar RNA (snoRNA) U16 (Figure 1). The approach we utilized is rapid and can be
carried out with transient transfection of the ribozyme expression units, bypassing the steps of
cloning and packaging the libraries in viral vectors. Moreover, stably transduced libraries often
suffer from a potent disadvantage in that the site of vector integration can determine the level
of expression of the ribozyme or small interfering RNA, oftentimes leading to heterogeneous
levels of ribozyme expression in cell populations. Our approach also takes advantage of a potent
survival assay using the herpes simplex virus thymidine kinase (HSV-TK) gene embedded in
infectious HIV-1 proviral DNA. Ribozymes that inhibit HIV-1 replication or gene expression
will render cells resistant to gancyclovir, while unprotected cells are killed following
phosphorylation of gancyclovir, converting it from a prodrug to a toxic nucleoside.
The use of a ribozyme library embedded in the U16 snoRNA stem provides two major
advantages. First, it provides a mechanism for colocalizing the ribozyme with full-length and
singly spliced viral RNA targets in the nucleolus.10 Second, the snoRNA portion provides a
PCR primer-binding site for retrieving the active ribozyme species from treated cells. We
demonstrate here the selection of three ribozyme sequences from a pool of 4 (ref. 14) U16
ribozyme chimeras that resulted in gancyclovir resistance of treated cells. Of these, one
sequence was isolated from three different colonies, suggesting a selective enrichment of this
ribozyme sequence. Sequence alignment with HIV-1 showed that the ribozyme could
potentially target two sites on the viral genome, at positions 550 and 4870 of HIV-1 NL4-3.
Both these sites were found to be very efficient for ribozyme-mediated inhibition, when tested
with perfectly matched ribozyme sequences. Inhibition of HIV-1 by the selected ribozyme and
its perfectly matched variants was further confirmed in CEM cells stably transduced with these
Unwalla et al. Page 2
Mol Ther. Author manuscript; available in PMC 2009 November 10.
NIH-PA Author Manuscript
NIH-PA Author Manuscript
NIH-PA Author Manuscript
Page 3
Selection of ribozymes that protect 293 cells from HSV-TK killing in the presence of
The ribozyme library was generated as described in the Materials and Methods section. Four
pools of ribozymes were generated for the screens. We used both positive and negative
selection approaches in our quest to isolate ribozymes inhibiting HIV-1 gene expression. The
selection approach used in our study involved cotransfecting HEK293 cells with infectious
proviral DNA pNL-TK (which has the HSV-TK inserted in the nef region of the HIV-1 genome)
and the ribozyme library. Twenty-four hours after transfection, the cells were treated with
gancyclovir and incubated for another 48 hours. The cells were then washed two times with
phosphate-buffered saline to remove dead cells in suspension and the total RNA was extracted
using STAT-60. The rationale for this approach is that, in the absence of any protective
ribozymes, HSV-TK would be expressed, resulting in cell killing and loss of ribozyme library
components in those cells, whereas survivors would contain the effective ribozymes. The
ribozymes were rescued from the total RNA with reverse transcriptase PCR using primers
complementary to the U16 stem. The PCR products containing the amplified ribozymes were
then recloned under the U6 promoter in the pTzU6 + 1 vector. This cycle of cotransfection and
selection was repeated three times. After each cycle, colonies were randomly selected and
sequenced to check for enrichment of particular sequences.
Identification of individual effective ribozymes that inhibit HIV-1 replication
After three rounds of selection, 50 colonies from pool I were randomly selected and sequenced.
As can be seen from Figure 2b, two ribozymes appeared to have been selectively enriched
during the pNL-TK-mediated screening process. Ten ribozymes including the two selectively
enriched ribozymes were directly tested in a pilot cotransfection assay with wild-type proviral
DNA pNL4-3. The U16 snoRNA under the U6 promoter was used as a negative control. The
results of this pilot experiment are depicted in Figure 2a. One of the sequences, isolated from
four different colonies, did not show any inhibition in p24 output, whereas RzC36 showed
good inhibition, as did RzC10, when compared with the U16 control-transfected cells. An
unexpected observation was that the ribozyme sequence C9 (RzC9) gave a higher p24 readout
as compared to the U16-transfected control.
Selected ribozymes inhibit pNL4-3 expression
The three selected ribozymes that showed anti-HIV-1 activity were retested in a cotransfection
assay with HIV-1 pNL4-3 using a higher ratio of ribozymes to HIV-1 vector. As can be seen
from Figure 2c, RzC36 yielded almost 70% inhibition of HIV-1 gene expression while RzC10
was moderately effective. RzC9 continued to show higher p24 levels as compared to the U16
controls. In order to better understand why RzC9 came through the selection process, we
cotransfected HEK293 cells with the selected ribozymes and HIV-1 pNL-TK. Forty-eight
hours after treatment of the cells with gancyclovir, total cells were collected, stained with trypan
blue, and the results expressed as a ratio of live/dead cells. U16-transfected cells were used as
a control. As can be seen from Figure 2d, RzC10-expressing cells had a moderate survival
advantage whereas both RzC9 and RzC36 conferred strong survival advantages. At this point
we have not been able to explain this paradox; however, it is quite possible that RzC9 could
actually be targeting a cellular factor involved in either viral RNA export or sorting of viral
RNA between translation and packaging.
RzC36 targets HIV-1 RNA
Using cDNA microarray experiments we sought to analyze gene expression patterns in
HEK293 cells as a function of HIV-1 pNL4-3 transfection and gene expression, and also
Unwalla et al.Page 3
Mol Ther. Author manuscript; available in PMC 2009 November 10.
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Page 4
investigated whether RzC36 altered this profile. We focused on genes that showed a plus or
minus log2 change of ~1.0. As can be seen in Figure 3, distinct changes in the expression
patterns of several genes are observed during viral replication. These changes could be a direct
or indirect result of viral gene expression in cells. We next compared this pattern with that
observed in cells cotransfected with HIV-1 pNL4-3 and RzC36. If the ribozyme's target site
was on the viral RNA, the patterns of altered gene expression might be expected to resemble
those found in non-HIV-1 pNL4-3-transfected cells. Interestingly, these analyses demonstrated
that all the virus-mediated changes observed with HIV-1 pNL4-3-transfected cells were
reversed in the presence of RzC36. Moreover, the extent of change (multiplying factor) in gene
expression produced by the RzC36-transfected cells was equal and opposite to that produced
by HIV-1 pNL4-3 transfection alone. Apart from the reversal of virus-mediated changes, no
other changes in the pattern of gene expression were observed in cells cotransfected with HIV-1
pNL4-3 and RzC36.
Having observed that RzC36 reversed the pattern of HIV-1-mediated changes in host gene
expression, we next sought to determine the potential ribozyme cleavage sites in the HIV-1
genome. Aligning the substrate recognition sequence of RzC36 with the HIV-1 NL4-3
sequence did not turn up any sites with perfect Watson–Crick base pairing. This is not an
unusual finding, in that Waninger et al. found similar mismatched pairings with their targets,
using a hairpin ribozyme library.21 These results can be attributed to the fact that the assays
used require a phenotypic change and do not necessarily require complete inhibition of the
target. We next devised an approach for target site determination on HIV-1 RNA that would
allow us to consider mismatches, bulges of 1 or 2 nucleotides (nt), and wobble base pairing.
We searched for targets containing an NU(A, C, or U) triplet flanked by at least 5 nt
complementary to helices 1 and 3 of RzC36 with mismatches in between. As can be seen in
Figure 4, we could identify two potential targets for RzC36 on the NL4-3 sequence. One site
was within the HIV-1-LTR in the R/U5 region at position 105. This site is unique, in that it is
very close to a previously reported, highly conserved site in the HIV-1 genome that was also
used by us in our earlier studies with a nucleolar-localized hammerhead ribozyme.10 A number
of studies including our own have shown efficient inhibition of HIV-1 gene expression by
targeting this region, thereby suggesting that the region is highly accessible for ribozyme or
antisense interaction.
The other site lies in the gag/pol open reading frame in the pol region at position 4870. The
U5 site would be present in spliced, un-spliced, and full-length forms of the transcript, whereas
the pol site would be present only in the full-length form. It is quite possible that the full-length
or partially spliced forms of the HIV-1 transcript, which are known to involve rev-mediated
export and thus, possibly, traffic through the nucleolus, could become targets for RzC36 during
this transit.
In order to determine which of the two sites was more highly accessible and therefore the true
target for RzC36, we designed variants of RzC36 that had perfect complementarity to each of
the two sites. Catalytically inactive variants were generated by mutating the C to a G in helix
II (Figure 4a and b). Both these ribozymes and their corresponding catalytic mutants were
tested in transient cotransfection assays with HIV-1 pNL4-3 into HEK293 cells. U16
cotransfected with HIV-1 pNL4-3 was used as a control. As can be seen from Figure 5, both
ribozyme variants produced pronounced inhibition of HIV-1 gene expression. Approximately
95% inhibition of HIV-1 p24 was observed with both the U5 and the Pol variants as compared
to the U16-transfected controls. Although we did observe some inhibition with the catalytically
inactive mutants, the inhibition with active ribozymes was much more pronounced, thereby
suggesting that the major reduction in viral output was caused by ribozyme-mediated catalytic
activity. The partial inhibition by the catalytically inactive variants could have been on account
of an antisense effect, given that these were also tightly colocalized with the HIV-1 targets. In
Unwalla et al.Page 4
Mol Ther. Author manuscript; available in PMC 2009 November 10.
NIH-PA Author Manuscript
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Page 5
order to check whether the decrease in p24 output was indeed caused by a ribozyme-mediated
cleavage rather than by a nonspecific block in viral translation, HEK293 cells were
cotransfected with HIV-1 pNL4-3 and either the Pol or U5 variants or the parent RzC36.
Culture supernatants were collected and analyzed using a B-DNA assay to check for the
presence of viral genomic RNA in the supernatant. As can be seen, nearly complete inhibition
of pNL4-3 RNA levels was observed in cells transfected with RzC36 or its perfectly matched
In order to check the efficiency of these ribozymes in a potential gene therapy setting, the U16
ribozyme cassettes (RzC36, U5 variant, and the Pol variant), were cloned in the lentiviral vector
pHIV-7-GFP and packaged as described earlier.22 CEM cells were transduced with either
vector alone, the perfectly matched ribozyme variants, or the parent RzC36, and challenged
with ~150 ng p24 equivalent of HIV-1 NL4-3 virus. At designated time-points the culture
supernatant was collected and analyzed for p24 levels as an index of viral replication. As can
be seen from Figure 4c, an average ~65–70% inhibition of HIV-1 gene expression was
observed. All three ribozymes showed a comparable level of inhibition, thereby suggesting
that both the U5 and the Pol target sites selected by our approach were efficient targets. It is
therefore quite possible that RzC36 was chosen for its ability to actually target both sites on
the viral RNA. This is not surprising, given that the potent TK-gancyclovir suicide axis would
require considerable knockdown of the TK gene to allow the cells to survive in the presence
of gancyclovir. This would ensure the selection and enrichment of only the most potent
ribozyme molecules from the library.
Anti-HIV-1 therapy using ribozymes has focused mainly on either targeting the viral RNA or
mRNA of cellular genes that may have a role to play in the viral life cycle. In order to achieve
a near complete knockdown of viral gene expression, one needs to take into account several
factors that may influence the cleavage efficiency of these antiviral agents. The most important
considerations are target site accessibility and colocalization of the ribozyme and target.
Although a number of guiding parameters are available to aid the selection of efficient target
sites, most of these parameters are established in vitro and then tested in cells. Most approaches
to ribozyme design begin with choosing an appropriate NU(A, C, or U) target site in the
message of interest. These approaches do not take into account factors that can alter the
efficiency of the ribozymes in an intracellular environment, such as local structural features in
the target mRNA and RNA–protein interactions. By using an in vivo or intracellular library
approach these concerns are minimized. Moreover, by modifying the selection process, one
can enrich for ribozymes that achieve the desired phenotype without necessarily requiring
complete target knockdown.
In this report, we have exploited the potent killing effect of the TK-gancyclovir axis in an
HIV-1 proviral DNA setting. The hammerhead ribozymes were incorporated in the U16
snoRNA stem to localize them to the nucleolus, an intracellular compartment that we found to
be effective for ribozyme targeting of singly spliced and unspliced HIV-1.10 We reasoned that
this approach would result in a large number of ineffective ribozymes being eliminated in the
first round of selection. Indeed, we saw almost ~80% of the cells detached from the surface
during the first round of selection. On the other hand, cells harboring a protective ribozyme
would inhibit expression of TK and survive. The ribozyme molecules from these cells could
then be rescued by reverse transcriptase-PCR of total RNA using primers specific to the U16
stem. After three rounds of selection with this approach, random sampling of the colonies
revealed two sequences that had been enriched during the process. We also randomly tested
other library ribozymes along with these two for their activity against HIV-1. We were
surprised to find one that routinely resulted in a higher p24 output as compared to the U16-
Unwalla et al.Page 5
Mol Ther. Author manuscript; available in PMC 2009 November 10.
NIH-PA Author Manuscript
NIH-PA Author Manuscript
NIH-PA Author Manuscript


text:
Zach Parise had two goals and an assist to help New Jersey tie the series.
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"I takes the devil dogs in this one. Expect the unexpected. "
Play - St. Louis Cardinals w/Lynn vs Fiers.
Edges - Cardinals: Lance Lynn 5-1 MLB career team starts in this park, and 13-5 MLB career team starts during April. Brewers: Michael Fiers 0-4 MLB career teams starts during April, and 0-2 last two team starts in this series.
With Fiers sporting a 6.75 ERA and a 1.74 WHIP this season, we recommend a 1-unit play on St. Louis. Thank you and good luck as always.
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iOS 5 Concept — Dynamic Icons and App Preview (youtube.com)
submitted 3 years ago by janmike34
[–]janmike34[S] 0 points1 point2 points 3 years ago (0 children)
More videos coming soon, leading up to WWDC '11 Keynote on Monday!
[–]j0wy 0 points1 point2 points 3 years ago (0 children)
At least you didn't put the Apple logo at the end.
[–]newbill123 0 points1 point2 points 3 years ago (0 children)
I like the concept if used simply (like the calendar's date being updated).
But I don't think this would be helpful. Every Starbucks, Target, B&N app around would junk up their icons and turn Springboard into a big mess that drains power when running and looks different from when it's not running.
I like the sentiment, but I doubt this is going to be the answer to the problem.
π Rendered by PID 6237 on app-64 at 2015-04-25 02:00:47.913740+00:00 running 058681a country code: US.


text:
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JVGS JVGS is an open source platform game that is not like the others. It is build on simple line structures loaded from svg files, giving the whole game a "sketched" and minimalistic look. Levels can be made with simple xml and lua files.
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Free FingerPrint Imaging Software Fingerprint Imaging Software -- fingerprint pattern classification, minutae detection, Wavelet Scalar Quantization(wsq) compression, ANSI/NIST-ITL 1-2000 reference implementation, baseline and lossless jpeg, image utilities, math and MLP neural net libs
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Sleep#: Save Power. No More Noise! Send computer to sleep after VLC, Windows Media Player, BBC iPlayer...
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EXcompCEL An easy to use Java Desktop Tool to compare Excel Files.
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STDF Solutions To provide free solutions for the manipulation of Standard Test Data Format (STDF) files. Such solutions will first entail reading/writing of STDF files, with later solutions including statistical/visualization tools as demand dictates.
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KiCad library wizard At the moment its simple command line tool to create nice looking schematic libraries in kicad. I hope that it would be usefull for someone. dont need to click anything just write/paste pin names and thats it.
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clic command line interface calculator
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Unix GNU Windows Packages to create a Unix like environment under MS-Windows
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Unreal Speccy high-performance and rich features ZX-Spectrum emulator for WinNT platform
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Total Annihilation: Twilight Total Annihilation: Twilight is a massive extension/expansion of Total Annihilation, painstakingly balancing old units and adding in new ones, and carries on where Absolute Annihilation left off. TA:T has over 500 units and is balanced for multi-player.
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forcedel ForceDel deletes files that are in use by other processes.
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QT Serial Port Driver QT driver class that supports serial port access on windows and linux / posix compliant systems. This system take advantage of QT's event driven archtecture.
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vBitty(VBTT) - vBulletin Torrent Tracker Customizable torrent tracker for vBulletin 3.7.x. & 3.8.x. VBTT 3 uses PHP integrated tracker(smaller sites) or XBT backend style(large sites with SSH access)
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Hamilton, Ohio Golf Courses
Click on any the golf course listings below to explore golf courses in Hamilton, Ohio. Find course information, driving directions, scorecards and Hamilton, Ohio golf instructors.
417 Hamilton New London RdHamilton, Ohio 45013-36540
2505 Eaton RdHamilton, Ohio 45013-97370
4780 Millikin RdHamilton, Ohio 45011-23900
PGA of America Championships
Pete Dye Course
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Westchester Country Club
Rye, N.Y.
Philadelphia Cricket Club
Philadelphia, Pa.
Whistling Straits
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text:
Here's the System Requirements for XP. If it doesn't meet or exceed all of those attributes, don't even try.
AnimeGalleries [dot] Net | AnimeWallpapers [dot] Com | AnimeLyrics [dot] Com | AnimePedia [dot] Com | AnimeGlobe [dot] Com |
Here's the System Requirements for XP. If it doesn't meet or exceed all of those attributes, don't even try.
Ehhh, I dunno if I'll stick around. We'll see.
The List of Hate, My self-indulgent journal-thing.
Last Post: Video Vomit 05/11/11
I saw your post on page 1, and I'm exactly not willing to try something I've never heard of [O_o possibly makes me sound out of date]; unless I research it more than what you've posted about it, I'm not going to try it.Originally Posted by aizmov
Even if it is "easy". What's easy to you may be difficult to others. The 95 will be handed down to my dad; the only thing he'll be doing on it is searching the web, and that occasional download of GEDCOM for family research, and possibly download pictures if he ever learns the concepts of Right-Click>Save As.
Or I guess now you can just drag and drop the pictures or file on your desktop? Huh...I still like the save as feature.
And damn the Processor! 166 Mhz...
Maybe I'll just go into Computer Building for Independent Study instead of Game Programming. I've always wanted a mini-supercomputer, as he says. He built one from scratch several years ago which runs about the same peak as the recent Dells the school bought, but he has to turn his on with an "arcade" red button.
DSL is guilt-free, just burn it to a CD and pop it in. Whenever you've had enough of it, just pop it out and restart. It will leave no trace of it whatsoever. Just like poping a videogame CD in your Playstation, no install is required, and when you're done you just pop it out and BOOM no trace is left.
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FOX Sports Exclusive
Adam Greenberg to bat for Marlins
Watch the unfortunate major-league debut by Adam Greenberg, who was hit by a pitch in the head in his only MLB at-bat.Near the end of a disappointing season, the Miami Marlins are making one of the most magnanimous gestures of this baseball year.
The Marlins said Thursday that they have signed outfielder Adam Greenberg to a one-day, major league contract. Greenberg, hit in the head with a pitch during his only big-league plate appearance seven years ago, will play for the Marlins against the New York Mets Tuesday — the next-to-last day of the season.
''Life's going to throw you curveballs — or fastballs in the back of your head,'' Greenberg said on a conference call Thursday morning. ''I got hit by one of them. And it knocked me down and I could have stayed there. I had a choice ... and I chose to get up and get back in the box.''
Greenberg, then a Chicago Cubs prospect, saw only one pitch during the fateful July 9, 2005, game against the Marlins. He experienced vertigo as a result of the pitch, thrown by reliever Valerio De Los Santos. Greenberg hasn't been back to the major leagues since.
Greenberg, 31, continued his career as a minor leaguer in the Cubs, Los Angeles Dodgers, Kansas City Royals and Los Angeles Angels organizations. He also played independent baseball, most recently for the Bridgeport Bluefish of the Atlantic League from 2008 through 2011. Greenberg also appeared in two games for Team Israel during this month's World Baseball Classic qualifier, walking in his lone trip to the plate.
"I'm extremely proud to extend this opportunity to Adam," Marlins owner Jeffrey Loria said in a news release. "He has earned this chance as his love and passion for the game never diminished, despite his career tragically being cut short. I look forward to seeing Adam step up to the plate and realizing his comeback dream next Tuesday night."
Marlins manager Ozzie Guillen said Greenberg may start.
''I might start him in left field and have him lead off,'' Guillen said before Thursday night's game in Atlanta. ''If he hits a home run, he stays!''
''They're trying to give this kid a dream come true,'' Guillen said. ''Why not give the kid a chance to be what he wants to be?''
Guillen said he has had a couple days to consider the best way to use Greenberg. He thinks it will be better to have Greenberg start than to find the right place in the game to come in as a pinch hitter.
''It's more important to me to win the game,'' Guillen said.
Marlins right-hander Carlos Zambrano was with the Cubs in 2005 and was used as a pinch runner for Greenberg. Zambrano said Thursday he did not remember his role in the game.
''He wasn't a fit for us,'' Cubs manager Dale Sveum said Thursday. ''I wish him the best.''
The Mets' probable starter on Tuesday will be Cy Young candidate R.A. Dickey.
''I know our guys will be on the top step clapping for him when he gets in the batter's box,'' Mets manager Terry Collins said Thursday when asked about Greenberg's story.
Greenberg is one of only two players in baseball history to be hit by a pitch in his first-and-only major league appearance and never take the field. Fred van Dusen endured that fate with Philadelphia in 1955.
Greenberg was the subject of a campaign called ''One At Bat,'' which lobbied teams to give him a second chance, since his first appearance in the majors did not count as an official at-bat, just merely a plate appearance.
''I just really want to make sure everyone understands that this is an amazing thing, for not just me but for a lot of people,'' Greenberg said.
He and Marlins outfielder Justin Ruggiano once were teammates with the Double-A Jacksonville Suns, playing together there in 2006.
''Dude can play,'' Ruggiano said on Twitter.
It is ironic how the Marlins have been involved in just about every aspect of Greenberg's story.
His lone plate appearance for the Cubs came in Miami. When he played earlier this month for Israel's entry in the qualifying round for the World Baseball Classic, he played and trained at the Marlins' complex in Jupiter, Fla. And now his comeback game will be in Miami, albeit a different park than where he faced that fateful pitch seven years ago.
''Going back to the scene of the crime but a different location, I kind of look at it as a new stadium, new start,'' said Greenberg, who drew a walk in his lone plate appearance for Israel in the WBC qualifying games. ''For me, it's just down the street, but it's a new opportunity. It's really cool and special to have the Marlins, of course, recognize all of this. And to have it come full circle with them, it's just so gratifying, rewarding and special.''
Greenberg faced De Los Santos again in 2011, hitting a single off him as a member of the Bridgeport Bluefish in the independent Atlantic League.
The Marlins say Greenberg will donate his one-day salary — a pro-rated share of the minimum contract is $2,623 — to the team's foundation, which will in turn donate to the Sports Legacy Institute, a group that furthers the study, treatment and prevention of the effects of brain trauma in athletes and others.
Baseball diamonds are a girl's best friend. Check out MLB's FOXiest fans and tweet us your photo.Greenberg said he's hopeful of getting a chance to play for some club in spring training next year. He also insisted that this is not a stunt.
''I'm no different or more special than anyone else,'' Greenberg said. ''It just so happened that my story was the Sunday Night Baseball game on ESPN and it was the first pitch I ever saw and I got hit in the back of the head. Tragedy for me, but it's part of the game.''
The Marlins said Greenberg will donate his one-day salary to the Sports Legacy Institute by way of the Marlins Foundation. SLI, the well-known organization founded by Christopher Nowinski and Dr. Robert Cantu, studies the crisis of brain trauma in sports and advocates for more proactive treatment of concussions.
The Associated Press contributed to this report.
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Rosenthal: Tanaka to Yankees restores natural order in baseball
Japanese ace Tanaka agrees to 7-year, $155 million deal with Yankees
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text: What a BINGO Night! Thank you to the PTA and the following teachers who participated in calling BINGO numbers: Mrs.Bresnahan, Mrs. Coyle, Ms. Kurtz, Mrs. Smith, Mrs. Cox, Mrs. Dumas, Mr. Coulon, Mrs. Chase, Ms. Tellier, Ms. Rousseau,Mrs. Demers, Mrs. Shaffer, Ms. Tulley, Mrs. Rivard, and Mrs. Schwalbe! It was a wonderful family night! ~Mrs. Matthews & Mrs. Vitagliano~


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- Hotels
- Austria Hotels
- Vienna Hotels
- Austria Trend Hotel Donauzentrum
You can collect Welcome Rewards nights hereWagramer Strasse 83-85, Vienna, Vienna, 1220, Austria, 0800-886-062 (New Zealand Toll Free)
Austria Trend Hotel Donauzentrum Rooms & Accommodation
Lowest priceNZ$76
Price Match GuaranteePrice Match Guarantee on hotel bookings. If you find a lower price on exactly the same stay elsewhere, we'll match it or you can cancel your booking without a penalty
4-star hotel with bar/lounge, near Danube Tower
- Free WiFi
Main amenities
- 137 guestrooms
- Breakfast available
- Bar/lounge
- Self parking
- 7 meeting rooms
- Airport shuttle
- 24-hour front desk
- Daily housekeeping
- ATM/banking services
- Laundry service
- Conference space
- Multilingual staff
What's around
- In Donaustadt
- Danube Tower (1.6 mi / 2.5 km)
- Riesenrad (2.8 mi / 4.4 km)
- Wiener Prater (3 mi / 4.8 km)
- Millennium City (3 mi / 4.8 km)
- Donau Zentrum (0.1 mi / 0.2 km)
Good3.6 / 5
It was quite disappointing as the room was not up to the mark. Toiletries such as soap,…
Apr 21, 2015General a nice and clean hotel with good price. Staff is friendly but not trained as…
Jan 18, 2015
TripAdvisor Traveller Rating: 3.5
From 138 reviewsTripAdvisor Traveller Rating
Enter dates to check prices
Hotel Info: 0800 456 081
from NZ$76- Executive Room, 2 Single Beds
- Classic Single Room, 1 Single Bed
- Executive Suite, 2 Single Beds
- Classic Double Room
- Business Room, 2 Single Beds
Recommended hotels
At a glance
Key facts
Hotel size
- This hotel has 137 rooms
- This hotel is arranged over 3 floors
- Check-in time starts at 2 PM
- Check-out time is noon
- Express check-in
Required at check in
- Credit card or cash deposit required
- Government-issued photo ID required
Travelling with others
Extra people
- Rollaway beds available, charges may apply*
- Free WiFi in public areas
- Free WiFi and wired Internet access in rooms
Optional extras
Self parking costs EUR 18 per night with in/out privileges
Buffet breakfast is offered for an extra charge of EUR 15 per person (approximately)
Pets are allowed for an extra charge of EUR 12 per pet, per night
Nearby Four Star Vienna hotels
- NH Danube City4 starsExcellent4.1 / 5From
NZ$140NZ$116 - Park Inn by Radisson Uno City Vienna4 starsExcellent4.4 / 5From NZ$140
- Ramada Hotel and Suites Vienna4 starsExcellent4.2 / 5From NZ$84
- Courtyard by Marriott Vienna Messe/Prater4 starsExcellent4.3 / 5From NZ$153
See more Four Star Vienna hotels
In the hotel
Food and drink
- Buffet breakfast daily (surcharge)
- Bar/lounge
Working away
- Number of meeting rooms - 7
- Conference space
- Meeting rooms
- Conference space size (feet) - 4306
- Conference space size (meters) - 400
- 24-hour front desk
- Concierge services
- Tours/ticket assistance
- Limo or Town Car service available
- Dry cleaning/laundry service
- Laundry facilities
- Free newspapers in lobby
- Luggage storage
- Number of buildings/towers - 1
- Elevator/lift
- ATM/banking
- Designated smoking areas
- Disabled parking
- Accessible path of travel
- Braille or raised signage
- In-room accessibility
In the room
Home comforts
- Minibar
- Bathrobes
- Iron/ironing board (on request)
Sleep well
- Hypo-allergenic bedding available
- Pillow menu
- Blackout drapes/curtains
Things to enjoy
- Separate sitting area
Freshen up
- Private bathroom
- Shower/tub combination
- Free toiletries
- Hair dryer
Be entertained
- Cable TV channels
Stay connected
- Desk
- Free newspaper
- Free WiFi
- Daily housekeeping
- In-room safe
Top Vienna hotels
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Nov 12, 2014 - Hotel & Apartments Klimt3 starsGood3.9 / 5From NZ$60
"I liked the Klimt theme (including lift doors) and the rooms were certainly satisfactory. It wasn't as easy to get to by public transport as expected, but possible with a bit of a…"
Sep 22, 2014 - Leonardo Hotel Vienna4 starsExcellent4.1 / 5From NZ$112
"Good friendly reception. Very comfortable king sized bed. Wifi service was not compatible with my iPhone, might have been me, but I don't think so. Excellent position near rail…"
Sep 20, 2014 - Hotel Regina4 starsExcellent4.2 / 5From NZ$119
"Was in Vienna for a conference and Hotel Regina was an excellent base. It was very well connected for transport (just one block to the metro line). Very near some of the scenic…"
Jul 29, 2014
Nearby hotels
Austria Trend Hotel Donauzentrum Rooms & Accommodation's small print
Optional extras
Self parking costs EUR 18 per night with in/out privileges
Buffet breakfast is offered for an extra charge of EUR 15 per person (approximately)
Pets are allowed for an extra charge of EUR 12 per pet, per night
We have included all charges provided to us by this hotel. However, charges can vary, for example, based on length of stay or the room you book.


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Police Ram House to End Hostage Standoff
Phoenix police rammed a house with an armored vehicle, to end a 12-hour hostage standoff. The suspect was found dead of a self-inflicted gunshot wound. (May 22)
advertisement | advertise on newsday
Michael Scotto launches campaign for Nassau DA
Mother's Day: A tribute to those struggling
Rangers drop Game 3 to Capitals
Vigil for NYPD Officer Brian Moore
NFL's Tim Green names East Northport teacher in book
Police: Man killed walking on parkway ramp
Cops: Motorcyclist killed in North Lindenhurst crash
Centereach woman rescued from house fire
Event urging immigration reform draws sparse turnout
Bodies of husband, wife found in Mastic Beach home
Designers talk show house at Mill Neck Manor
Many arrested in NYC protests


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Plus Willie announces release of signature cannabis strain, Willie's Reserve.
GAC Follows Brooks & Dunn
Brooks & Dunn is giving fans a glimpse of their road life in a new GAC special, Brooks & Dunn: Cowboy Town, Aug. 13 at 9 p.m. ET.
The special follows Kix Brooks and Ronnie Dunn to various locations around the country, including a Dallas club and a rocking show in St. Louis. "Over the years, we've done several TV specials," says Kix, "but never actually filmed parts of a 'real show,' and certainly never this raw, in terms of behind the scenes. I think fans will enjoy it." Ronnie adds, "We made sure that when you're watching, you feel transported—like you were in the front row."
More News
Here are our 10 favorite moments from the big party in Dallas.
The 2015 ACM Awards airs live from Texas on Sunday, April 19.
Participates in world's longest truck parade and plays a private show at Gilley's.
Lee Brice, Will Hoge and others perform.
Rhett Akins takes Top Archer in ACM-related event.


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Muse Restaurant and Wine Bar
American · $$$
82 Society St
"Fantastic atmosphere, located in a beautiful historic home with many of the original details. Try the gnocci with gorgonzola and walnuts, it's the best."
Coast Bar and Grill
Seafood · $$$
39 John St
"The tuna entree w soba noodles is dank. Grouper w truffle oil is amazing. Cipionno was not my fave, but everything else was great! Jeremy & Lacey are fantastic servers!"
Mellow Mushroom
Pizza · $$
309 King St
"Do-it-yourself. Ranch base, buffalo chicken, fresh basil, tomato, and jalepenos. I call it the "rainmaker" absolutely delish!"
Make sure your information is up to date. Plus use our free tools to find new customers.
Download Foursquare for your smart phone and start exploring the world around you!


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By Pat Romanski | Article Rating: |
August 17, 2014 07:45 PM EDT | Reads: |
4,323 |
@ThingsExpo billboard is viewed by more than 1.3 million motorists per week on Highway 101, in the heart of Silicon Valley.
The Evolution of the Internet Of Things Session by Chris Gray
The Internet of Things is not new. Historically, smart businesses have used its basic concept of leveraging data to drive better decision making and have capitalized on those insights to realize additional revenue opportunities. So, what has changed to make the Internet of Things one of the hottest topics in tech?
In his session at Internet of @ThingsExpo, Chris Gray, Director, Embedded and Internet of Things, will discuss the underlying factors that are driving the economics of intelligent systems. Discover how hardware commoditization, the ubiquitous nature of connectivity, and the emergence of Big Data and analysis are providing the pull to meet customer expectations of a widely connected, multi-dimensional universe of people, things, and information. Chris will present real-world use cases that demonstrate how to:
- Collapse the information lifecycle and ultimately go from data to decision faster.
- Collect, transmit, transform, and take action on data in areas that have not been possible before.
- Meet critical security requirements as more and more information and data processing occurs outside the data center.
- Capitalize on near field processing to reduce transmission costs and decision time horizons.
Speaker Bio:
At Red Hat, Chris Gray has global responsibility for strategic partner initiatives and worldwide sales within the embedded and Internet of Things market. His extensive experience with hundreds of embedded partners and customers across numerous and diverse industries has given him a unique perspective on this explosive growth area. He has led the global expansion of Red Hat's Embedded and Internet of Things business in over twenty countries and continues to lead Red Hat's mission as the open source leader in embedded and intelligent systems solutions.
Chris has been with Red Hat for over six years, holding leadership positions in multiple global and regional business development and sales roles. Prior to Red Hat, he served as an Officer in the US Navy; an educator at Virginia Military Institute and a military leader aboard nuclear submarines. He graduated with distinction from the US Naval Academy with a degree in Computer Science, earned a Masters in Engineering Management from Old Dominion University, and is a Department of Energy certified nuclear engineer.
Click Here to View Video on YouTube
"The Internet of Things is the next wave of cloud. As these distributed devices become more prolific they can really start to gain a lot more insight and gather more date from the environment," explained Chris Gray, Director of Embedded Intelligence Systems at Red Hat, in this SYS-CON.tv interview at the Internet of @ThingsExpo, held June 10-12, 2014, at the Javits Center in New York City.
Intelligent Systems in Transportation Session by James Kirkland
Intelligent systems may share common architectures, but they can solve a variety of challenges - from increasing accuracy with a rail signaling system to improving safety with an air traffic control system. In the transportation industry, addressing these challenges is particularly critical in order to ensure safety, increase efficiency, and reduce costs.
In his session at @ThingsExpo, James Kirkland, an Enterprise Solutions Architect at Red Hat, will discuss real-world use cases in the transportation industry and how open source technologies can create integrated, interoperable, and reliable systems of thousands of devices. He will also cover:
- Optimized routing and fuel management with a combination of resource location tracking and business rules.
- Telecommunications utilization and availability through multi-path link management.
- Decision-making in the field to decrease reaction time and mis-routing of assets.
- Real time communication to improve safety
- Data analysis to predict equipment failures
James Kirkland is an Enterprise Solutions Architect at Red Hat. He has been working with Unix variants for 14 years. James is Linux LPIC level one certified, an RHCE, and a HP-UX certified System Administrator. He has been working with Linux for nine years, VMware for four years, and hp-ux for ten years. He has been a participant at HP World, Linux World, and numerous internal HP forums. He is the co-author of Linux(R) Troubleshooting for System Administrators and Power Users (ISBN: 0131855158) published by PrenticeHall PTR.
3rd International Internet of @ThingsExpo will take place November 4-6, 2014 at the Santa Clara Convention Center, Santa Clara, California with over 100 major sponsors and exhibitors in 2014 and more than 6,000 delegates from 17 countries.
Internet of @ThingsExpo this week launched the premiere issue of its monthly newsletter which contains highlights of the hottest sessions and speakers from Internet of @ThingsExpo, speaking information as well as sponsorship opportunities.
Download @ThingsExpo Newsletter ▸ Here
Chris Matthieu Named Internet of @ThingsExpo Tech Chair
Internet of @ThingsExpo has announced today that Chris Matthieu has been named tech chair of Internet of @ThingsExpo 2014 Silicon Valley.
The 2nd Global Internet of @ThingsExpo will take place on November 4-6, 2014, at the Santa Clara Convention Center in Santa Clara, CA.
Chris Matthieu has two decades of telecom and web experience. He launched his Teleku cloud communications-as-a-service platform at eComm in 2010, which was acquired by Voxeo. Next he built an open source Node.JS PaaS called Nodester, which was acquired by AppFog. His new startup is Twelephone (http://twelephone.com). Leveraging HTML5 and WebRTC, Twelephone's BHAG (Big Hairy Audacious Goal) is to become the next generation telecom company running in the Web browser.
Tracks and topics for Internet of Internet of @ThingsExpo Silicon Valley will include:
Consumer IoT and Wearables Track
- Smart Appliances
- Wearables
- Smart Cars
- Smartphones 2.0
- Smart Travel
- Personal Fitness
- Health Care
- Personalized Marketing
- Customized Shopping
- Personal Finance
- The Digital Divide
- Mobile Cash & Markets
- Games & the IoT
- The Future of Education
- Virtual Reality
Video: @ThingsExpo power panel discussion at Internet of @ThingsExpo New York
Enterprise IoT Track
- The Business Case for IoT
- Smart Grids
- Smart Cities
- Smart Transportation
- The Smart Home
- Authentication/Security
- Wiring the IoT
- The Internet of Everything
- Digital Transformation of Enterprise IT
- Agriculture
- Transportation
- Manufacturing
- Local & State Government
- Federal Government
IoT Developer and WebRTC Track
- Eclipse Foundation
- Cloud Foundry
- Linux Containers
- Node-Red
- Open Source Hardware
- AJAX and the IoT
- Leveraging SOA
- Multi-Cloud IoT
- Evolving Standards
- WebSockets
- Security & Privacy Protocols
- GPS & Proximity Services
- Bluetooth/RFID/etc.
- Nest Labs
@ThingsExpo billboard is viewed by more than 1.3 million motorists per week on Highway 101, in the heart of Silicon Valley.
Internet of @ThingsExpo 2014 Silicon Valley "show prospectus" has shipped. Sponsorship, exhibit, and keynote opportunities can be obtained from Carmen Gonzalez by email at events (at) sys-con.com, or by phone 201 802-3021.
Early Bird Registration Options
for Internet of @ThingsExpo 2014 Silicon Valley
Internet of @ThingsExpo delegates can pre-register for Internet of @ThingsExpo with Early Bird Savings here.
About SYS-CON Media & Events
SYS-CON Media (www.sys-con.com) has since 1994 been connecting technology companies and customers through a comprehensive content stream at www.sys-con.com - featuring over forty focused subject areas, from Cloud Computing to Web Security - interwoven with market-leading full-scale conferences produced by SYS-CON Events. The company's internationally recognized brands include among others Cloud Expo® (www.CloudComputingExpo.com), Big Data Expo (http://BigDataExpo.net), Virtualization Conference & Expo (www.VirtualizationConference.com), DevOps Summit (http://DevOpsSummit.sys-con.com), Internet of @ThingsExpo (http://www.ThingsExpo.com), SDDC Expo (http://SDDCExpo.sys-con.com), WebRTC Summit (http://WebRTCSummit.net), Government IT Conference & Expo (www.GovITExpo.com), Cloud Computing Bootcamp (www.CloudComputingBootcamp.com), and UlitzerLive! New Media Conference & Expo (http://events.sys-con.com).
Cloud Expo® is a registered trademark of Cloud Expo, Inc.
Published August 17, 2014 Reads 4,323
Copyright © 2014 SYS-CON Media, Inc. — All Rights Reserved.
Syndicated stories and blog feeds, all rights reserved by the author.
More Stories By Pat Romanski
News Desk compiles and publishes breaking news stories, press releases and latest news articles as they happen.
17th Cloud Expo, taking place Nov 3-5, 2015, at the Santa Clara Convention Center in Santa Clara, CA, will feature technical sessions from a rock star conference faculty and the leading industry players in the world.
Cloud computing is now being embraced by a majority of enterprises of all sizes. Yesterday's debate about public vs. private has transformed into the reality of hybrid cloud: a recent survey shows that 74% of enterprises have a hybrid cloud strategy. Meanwhile, 94% of enterprises are using some form of XaaS – software, platform, and infrastructure as a service. May. 6, 2015 04:45 PM EDT Reads: 791 |
By Elizabeth White Enterprise IoT is an exciting and chaotic space with a lot of potential to transform how the enterprise resources are managed.
In his session at @ThingsExpo, Hari Srinivasan, Sr Product Manager at Cisco, will describe the challenges in enabling mass adoption of IoT, and share perspectives and insights on architectures/standards/protocols that are necessary to build a healthy ecosystem and lay the foundation to for a wide variety of exciting IoT use cases in the years to come. May. 6, 2015 04:30 PM EDT Reads: 1,277 |
By Elizabeth White Avnet, Inc. has announced that it ranked No. 4 on the InformationWeek Elite 100 – a list of the top business technology innovators in the U.S. Avnet was recognized for the development of an innovative cloud-based training system that serves as the foundation for Avnet Academy – the company's education and training organization focused on technical training around top IT vendor technologies. The development of this system allowed Avnet to quickly expand its IT-related training capabilities around the world, while creating a new service that Avnet and its IT solution providers can offer to their... May. 6, 2015 02:45 PM EDT Reads: 1,049 |
By Carmen Gonzalez @ThingsExpo has been named the Top 5 Most Influential M2M Brand by Onalytica in the 'Machine to Machine: Top 100 Influencers and Brands.' Onalytica analyzed the online debate on M2M by looking at over 85,000 tweets to provide the most influential individuals and brands that drive the discussion. According to Onalytica the "analysis showed a very engaged community with a lot of interactive tweets. The M2M discussion seems to be more fragmented and driven by some of the major brands present in the M2M space. This really allows some room for influential individuals to create more high value inter... May. 6, 2015 12:15 PM EDT Reads: 5,876 |
By Liz McMillan SYS-CON Events announced today that B2Cloud, a provider of enterprise resource planning software, will exhibit at SYS-CON's 16th International Cloud Expo®, which will take place on June 9-11, 2015, at the Javits Center in New York City, NY.
B2cloud develops the software you need. They have the ideal tools to help you work with your clients. B2Cloud's main solutions include AGIS – ERP, CLOHC, AGIS – Invoice, and IZUM May. 6, 2015 12:00 PM EDT Reads: 4,532 |
By Liz McMillan It's time to put the "Thing" back in IoT. Whether it's drones, robots, self-driving cars, ...
There are multiple incredible examples of the power of IoT nowadays that are shadowed by announcements of yet another twist on statistics, databases, .... Sorry, I meant, Big Data(TM), tiered storage(TM), complex systems(TM), smart nations(TM), ....
In his session at WebRTC Summit, Dr Alex Gouaillard, CTO and Co-Founder of Temasys, will discuss the concrete, cool, examples of IoT already happening today, and how mixing all those different sources of visual and audio input can make your life happier ... May. 6, 2015 11:15 AM EDT Reads: 755 |
By Pat Romanski The WebRTC Summit 2015 New York, to be held June 9-11, 2015, at the Javits Center in New York, NY, announces that its Call for Papers is open. Topics include all aspects of improving IT delivery by eliminating waste through automated business models leveraging cloud technologies. WebRTC Summit is co-located with 16th International Cloud Expo, @ThingsExpo, Big Data Expo, and DevOps Summit. May. 6, 2015 11:15 AM EDT Reads: 3,248 |
By Liz McMillan There is no doubt that Big Data is here and getting bigger every day. Building a Big Data infrastructure today is no easy task. There are an enormous number of choices for database engines and technologies. To make things even more challenging, requirements are getting more sophisticated, and the standard paradigm of supporting historical analytics queries is often just one facet of what is needed. As Big Data growth continues, organizations are demanding real-time access to data, allowing immediate and actionable interpretation of events as they happen. Another aspect concerns how to deliver ... May. 6, 2015 11:00 AM EDT Reads: 5,891 |
By Elizabeth White SYS-CON Events announced today that MangoApps will exhibit at SYS-CON's 16th International Cloud Expo®, which will take place on June 9-11, 2015, at the Javits Center in New York City, NY., and the 17th International Cloud Expo®, which will take place on November 3–5, 2015, at the Santa Clara Convention Center in Santa Clara, CA.
MangoApps provides private all-in-one social intranets allowing workers to securely collaborate from anywhere in the world and from any device. Social, mobile, and easy to use. MangoApps has been named a "Market Leader" by Ovum Research and a "Cool Vendor" by Gartner... May. 6, 2015 11:00 AM EDT Reads: 4,471 |
By Carmen Gonzalez The world's leading Cloud event, Cloud Expo has launched Microservices Journal on the SYS-CON.com portal, featuring over 19,000 original articles, news stories, features, and blog entries.
DevOps Journal is focused on this critical enterprise IT topic in the world of cloud computing.
Microservices Journal offers top articles, news stories, and blog posts from the world's well-known experts and guarantees better exposure for its authors than any other publication.
Follow new article posts on Twitter at @MicroservicesE May. 6, 2015 11:00 AM EDT Reads: 2,996 |
By Roger Strukhoff Containers and microservices have become topics of intense interest throughout the cloud developer and enterprise IT communities.
Accordingly, attendees at the upcoming 16th Cloud Expo at the Javits Center in New York June 9-11 will find fresh new content in a new track called PaaS | Containers & Microservices
Containers are not being considered for the first time by the cloud community, but a current era of re-consideration has pushed them to the top of the cloud agenda. With the launch of Docker's initial release in March of 2013, interest was revved up several notches. Then late last... May. 6, 2015 11:00 AM EDT Reads: 4,025 |
By Liz McMillan WebRTC defines no default signaling protocol, causing fragmentation between WebRTC silos. SIP and XMPP provide possibilities, but come with considerable complexity and are not designed for use in a web environment.
In his session at @ThingsExpo, Matthew Hodgson, technical co-founder of the Matrix.org, discussed how Matrix is a new non-profit Open Source Project that defines both a new HTTP-based standard for VoIP & IM signaling and provides reference implementations. May. 6, 2015 10:45 AM EDT Reads: 4,494 |
By Liz McMillan In his session at WebRTC Summit, Peter Dunkley, Technical Director at Acision, will look at creating interactive communications via the web by adding messaging, file transfer, and group communication (group chat and audio/video conferencing) into the web experience. He will also discuss potential applications of this technology in areas including B2B, B2C, P2P, and gaming.
Peter Dunkley is Technical Director at Acision. He graduated from The University of Edinburgh in 2000 with a BSc (Hons) in Computer Science. After graduation Peter worked on a PSTN switch developing signalling stacks for SS... May. 6, 2015 10:30 AM EDT Reads: 723 |
By Elizabeth White The security devil is always in the details of the attack: the ones you've endured, the ones you prepare yourself to fend off, and the ones that, you fear, will catch you completely unaware and defenseless. The Internet of Things (IoT) is nothing if not an endless proliferation of details. It's the vision of a world in which continuous Internet connectivity and addressability is embedded into a growing range of human artifacts, into the natural world, and even into our smartphones, appliances, and physical persons.
In the IoT vision, every new "thing" - sensor, actuator, data source, data con... May. 6, 2015 10:30 AM EDT Reads: 5,499 |
By Liz McMillan What if, during a snow emergency, an on-the-ground sensor could automatically trigger a relevant emergency notification related to snowfall and road impact. And then, after it's triggered, that notification is delivered intelligently to individuals based on an extensive set of rules designed to alert the most available and capable responders.
This "what if" question about "smart highways" is short-sighted. We are already there, and we are only getting started. While mainstream attention is paid to machine-to-machine communications, new technologies are being developed to make these communica... May. 6, 2015 10:26 AM EDT Reads: 561 |
By Elizabeth White The Internet of Things is not new. Historically, smart businesses have used its basic concept of leveraging data to drive better decision making and have capitalized on those insights to realize additional revenue opportunities. So, what has changed to make the Internet of Things one of the hottest topics in tech?
In his session at @ThingsExpo, Chris Gray, Director, Embedded and Internet of Things, discussed the underlying factors that are driving the economics of intelligent systems. Discover how hardware commoditization, the ubiquitous nature of connectivity, and the emergence of Big Data a... May. 6, 2015 10:00 AM EDT Reads: 5,659 |
By Carmen Gonzalez SYS-CON Media announced today that @WebRTCSummit Blog, the largest WebRTC resource in the world, has been launched.
@WebRTCSummit Blog offers top articles, news stories, and blog posts from the world's well-known experts and guarantees better exposure for its authors than any other publication.
@WebRTCSummit Blog can be bookmarked ▸ Here
@WebRTCSummit conference site can be bookmarked ▸ Here May. 6, 2015 09:45 AM EDT Reads: 3,495 |
By Liz McMillan Scott Jenson leads a project called The Physical Web within the Chrome team at Google. Project members are working to take the scalability and openness of the web and use it to talk to the exponentially exploding range of smart devices. Nearly every company today working on the IoT comes up with the same basic solution: use my server and you'll be fine. But if we really believe there will be trillions of these devices, that just can't scale. We need a system that is open a scalable and by using the URL as a basic building block, we open this up and get the same resilience that the web enjoys. May. 6, 2015 09:45 AM EDT Reads: 6,052 |
By Yeshim Deniz Chuck Piluso will present a study of cloud adoption trends and the power and flexibility of IBM Power and Pureflex cloud solutions.
Speaker Bio: Prior to Secure Infrastructure and Services, Mr. Piluso founded North American Telecommunication Corporation, a facilities-based Competitive Local Exchange Carrier licensed by the Public Service Commission in 10 states, serving as the company's chairman and president from 1997 to 2000.
Between 1990 and 1997, Mr. Piluso served as chairman & founder of International Telecommunications Corporation, a facilities-based international carrier licensed ... May. 6, 2015 09:00 AM EDT Reads: 938 |
By Yeshim Deniz There are lots of challenges in IoT around secure, scalable and business friendly infrastructure for enterprises.
For large corporations, IoT implementations are one of the top priorities of the decade. All industries are seeing a competitive need to sustain by investing in IoT initiatives.
The value addition comes from improved customer service, innovative product and additional revenue streams.
The data from these IP-connected devices can be leveraged for a variety of business applications as well as responsive action controls.
The various architectural building blocks of an IoT ... May. 6, 2015 09:00 AM EDT Reads: 1,195 |


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Ontarians will take transit over beer: Poll
Ontarians like the idea of spending on infrastructure -- even more than beer in grocery stores.
Wynne's approval numbers drop
There's no Wynne in the Liberal's sails.
Time for Ontario Tories to choose their future
Old guard or new guard?
Is Ontario headed for a debt crisis?
Ontario would be a loan shark's dream.
Crane company fined after fire that trapped man
KINGSTON, Ont. -- The employer of a crane operator who was trapped for hours atop a crane above a massive fire has been fined a total of $27,000 for failing to co-operate with the Ministry of Labour's investigation.
10 Ford cars damaged in truck stop blaze
FORT ERIE, Ont. -- Damage is estimated around $500,000 after a transport truck carrying 10 Ford Edges went up in flames at a Fort Erie, Ont., truck stop Friday morning.
Tory MPP attacks Elliott over sex ed
Progressive Conservative MPP Monte McNaughton has has accused Christine Elliott — a frontrunner his party's leadership race — of "quietly cheering on" the controversial new Liberal sex-ed curriculum.
Ont. teacher accused of voyeurism fired
A special education teacher accused of secretly recording women in a school change room has been fired, Postmedia Network has learned.
'Driving drunk woo' woman texts before fatal crash
A Miami woman charged with DUI manslaughter allegedly texted her boyfriend "Drunk driving woo" just minutes before a fatal collision that killed her passenger.


text: I'm not a big fan of Chinese - but maybe it's because I have had much "good" Chinese. Azu is not just good, it is absolutely amazing! Been twice now. Salt and pepper shrimp first time - pineapple and strawberry shrimp this time - and I can not say enough! We've had dessert and sushi - and all... read more


text:
My ethnicity is | Black |
Height | 5'1" or below |
Status | Single |
Seeking | Friends/Long-term |
Body type | Average build |
Have children | No children |
Smoking | Hate smoking |
Drinking | Never drink |
Nickname | Skidmolly, Woman, aged 39 |
Location | Burnaby, British Columbia, Canada |
Looking for a | Man, aged 32 - 38, White/Islander/Latino... (Preferred) |
My ethnicity is | Black |
Height | 5'1" or below |
Status | Single |
Seeking | Friends/Long-term |
Body type | Average build |
Have children | No children |
Smoking | Hate smoking |
Drinking | Never drink |
How would you describe yourself?
I enjoy being a woman - yes I enjoy being affectionate and caring and crying when something touches my heart. You will know my honest nature by seeing me do the things I actually say. I am not a "Spin Doctor", I don't play games when it comes to matters of the heart.
I like to play and watch sports. I like to get down and dirty to fix or build things. I absolutely love music and dancing. I usually listen to music 24/7 but I can appreciate solitude as well. I am not a clubber but I enjoy getting my groove on now and then especially Salsa dancing.
Laughing is awesome!! I love laughing to the point it makes your cry or pee your pants... Yes it can happen!!
I am looking for
It takes the right man to know that he's in the presence of a classy lady. I appreciate chivalry because that's a true sign of a gentleman. So boys need not apply!!
I believe we can create happiness and we can bless others with it as well. I look forward to being inspired by you to try new things, experience new point of views, and most of all, inspire me to be a better person.
If you are a man who is spontaneous, loves people, loves a good prank, seeks after truth and live by its principles then throw in your line... I just may bite!!
About Her
Starsign | Capricorn |
Want children | Want children |
Eye color | Brown |
Hair color | Black |
Religion | Christian (LDS) |
Occupation | Other |
Education | Graduate degree |
Languages | English |
Relocation | I'd relocate anywhere in the world |
Ideally I'd live in a | House in the suburbs/Property in the country/Beach house |
My fashion sense is | Wear the latest trends |
My sense of humor is | Witty - I have a razor-sharp tongue |
When I go to parties | I get around |
On a day off, I enjoy | Something athletic or energetic/A cultural outing such as museum or gallery/Curling up with a good book/Lunch with a friend... |
I attend religious services | Once a week |
When it comes to work | I keep a standard 40-hour work week |