text:
|Insomnia affects up to 40 percent of American adults and for women going through menopause, a drop in hormones, especially estrogen, can exacerbate sleeplessness, according to hormone expert Marla Ahlgrimm. Ahlgrimm explains that one of the first symptoms of the decline in estrogen is sleep disturbance. Estrogen affects the metabolic processes in the brain and promotes deep sleep as well as the so-called REM periods in which we dream and process experiences.
The National Sleep Foundation has found that approximately 61% of menopausal women have sleep problems. But these problems can occur 10 years in advance of menopause, when a woman is in her early 40's, explains Marla Ahlgrimm.


text:
Racecar Driving Experience
Charlotte Motor Speedway • Concord, NC
Select Dates in Dates in February, March, June, July, November, and December
What you get
- Three Laps with a Pro Driver
- Or, Take the Wheel For a Six-Lap Driving Experience
- Choice of Dates in February, March, June, July, November, and December
Racecar pajamas might excite your 5-year-old nephew, but the grown-ups on your gift list will appreciate slipping into the real thing with a deal from The Racing Experience: either a three-lap ride-along package, or a six-lap qualifier driving package at Charlotte Motor Speedway.
After a driver's meeting, some basic instruction, and a few warm-up laps, the driving package will have you racing at thrilling high speeds in an authentic, race-proven stock car with other student drivers. Passing is allowed from the drop of the green flag, along with in-car radio instruction from your spotter. If you're not quite ready to take the wheel, the ride-along package packs plenty of punch for the passenger, as the driver maneuvers past competitors and takes sweeping turns that'll make you feel young, wild, and free.
Choose from the following offers:
• $64 ($129 value) for a three-lap ride-along package
• $199 ($399 value) for a six-lap qualifier driving package
The Racing Experience's Website
This was awesome! I would go back everyday if I could!"
LivingSocial Member
Fine printREFUND POLICY: This event is nonrefundable. No full or partial refunds are given to customers who miss an event. In the event of a non-weather-related cancellation, your voucher will be fully refunded. • Limit 4 per purchaser • Each voucher is good for 1 person • Must be 18+ with valid driver's license • Must be within 5' - 6'4" with a waist of 52" or smaller • Reservations required and subject to availability • The The deal is valid for any 2014 date based on availability, reservations are on a first come first served basis, dates are subject to change • Merchant cancellation/re-scheduling policy applies. A rescheduling fee equal to up to one half the voucher value may be applied within 30 days of your event. For weather-related cancelations, you will have 1 calendar year to reschedule; voucher subject to forfeiture • Optional $1,000 max property damage financial liability insurance available to purchase from merchant before driving for $60 • Merchant terms apply: www.racewithrusty.com/terms • Entire value must be used in a single visit
Racecar Driving Experience
times for
More upcoming events
Thursday Apr 23 through Thursday Apr 30
Charlotte, NC
2 Tickets to Show at Actor's Theatre of Charlotte
Actor's Theatre of Charlotte
use by Nov 29
Super Speedway Track or Dirt Driving Experience
Charlotte Motor Speedway | Carolina Speedway
use by Oct 1
Paint Nite Painting Event at Local Pub or Restaurant
Saturday Apr 11 through Sunday Apr 12
Concord, NC
Entry Package to Charlotte Sprint Spartan Race
Porter Farms
Sunday Apr 12
Raleigh, NC
Rock 'n' Roll Raleigh 1/2 Marathon or Marathon
West Davie Street and South Salisbury Street
use by 2016
Concord, NC
Mario Andretti Racing Experience
Charlotte Motor Speedway
Saturday May 30
Greenville, SC
2 Tickets to the Hey Look! Music Festival
Thomas Creek Brewery
use by Jun 27
Charlotte, NC
First Time Brewers Course
Seven Jars Products
use by Jul 14
Charlotte, NC
Kid Rock with Foreigner on July 14
PNC Music Pavilion
Friday May 15
Charlotte, NC
Ticket to "The Sweet Spot: Make It Rain Edition"
The Chop Shop NoDa
Saturday Apr 11
Charlotte, NC
2 Tickets to the 3rd Annual Zombie Bar Crawl
Tilt on Trade
use by 2016
Concord, NC
NASCAR Racing Ride-Along or Driving Experience
Charlotte Motor Speedway
use by Jul 7
Charlotte, NC
Imagine Dragons with Metric on July 7
Time Warner Cable Arena
use by Jun 13
Charlotte, NC
Lana Del Rey Concert on June 13
PNC Music Pavilion
use by Dec 5
Charlotte, NC
Billy Joel Concert on December 5
Time Warner Cable Arena
use by Jun 17
Charlotte, NC
Hozier with Dawes on June 17
Uptown Amphitheatre at NC Music Factory
use by Sep 9
Charlotte, NC
5 Seconds of Summer Concert on September 9
PNC Music Pavilion
use by Jun 6
Charlotte, NC
Barry Manilow with Dave Koz on June 6
Time Warner Cable Arena
use by Jul 19
Charlotte, NC
Shania Twain with Gavin DeGraw on July 19
Time Warner Cable Arena
use by Jul 18
Charlotte, NC
Sam Smith Concert on July 18
Time Warner Cable Arena
use by Aug 29
Charlotte, NC
Country Megaticket: Darius Rucker, Brothers Osborne, and More
Verizon Wireless Amphitheatre
use by Aug 7
Charlotte, NC
Lady Antebellum Concert on August 7
PNC Music Pavilion
use by Jun 11
Charlotte, NC
Kenny Chesney with Chase Rice
Time Warner Cable Arena
use by May 31
Charlotte, NC
Earth, Wind & Fire Concert on May 31
Uptown Amphitheatre at NC Music Factory
use by Jul 19
Charlotte, NC
Fall Out Boy with Wiz Khalifa and Hoodie Allen on July 19
PNC Music Pavilion
use by Jul 15
Charlotte, NC
CONCACAF Gold Cup on July 15
Bank of America Stadium
use by Aug 28
Charlotte, NC
Nickelback with Lifehouse on August 28
PNC Music Pavilion
use by Aug 4
Charlotte, NC
Nicki Minaj with Meek Mill and Tinashe on August 4
PNC Music Pavilion
use by Jun 4
Charlotte, NC
Zac Brown Band Concert on June 4
PNC Music Pavilion
use by 2016
3-Course Murder Mystery Dinner for 1 or 2
Saturday Aug 29
Black Mountain, NC
Entry Package for Asheville Spartan Super
Grove Stone & Sand Company
use by Nov 7
Rock Hill, SC
Entry to Insane Inflatable 5K
Winthrop University
use by Jun 8
Charlotte, NC
Taylor Swift with Vance Joy on June 8
Time Warner Cable Arena
Nearby deals
Triangle Pest Control
One Year of Pest Control Services
Five Pilates or CrossFit Classes
Charlotte Natural Healing
One, Four, or Eight i-Lipo Ultra Sessions
Dolce Ristorante
$40 to Spend on Italian Cuisine
Lake Norman Hypnotherapy and Coaching Center
One or Two Hypnosis Sessions
Secure Turf
Landscaping Consultation
Life 2 Luxury
1- or 2-Hour Photo Package; or Wedding Photography
Machu Picchu Restaurant
$20 to Spend on Peruvian Cuisine and More

subset: commoncrawl


text:
A child or other individual for whom a parent, relative, or other person may claim a personal exemption tax deduction. Under the Affordable Care Act, individuals may be able to claim a premium tax credit to help cover the cost of coverage for themselves and their dependents.
Get ready for the next Open Enrollment period. Sign up for email and text updates to get deadline reminders and other important information.
Now that you've signed up for updates from the Health Insurance Marketplace, we'll send you tips and reminders to help you get covered.
February 15 was the last day to enroll in or change a health plan for 2015. But you may still be able to get covered.


text:
Agreement was reached earlier today between US and Russia on Syria's disarmament with Syria approval. This extremely significant agreement should have positive influence on next week stock market. This should contribute to NOK farther pps growth continuation. Bullish sign. So stay bullish !
What a defeat for shorts. Terrible news for all those, who bet on war. Looks like another very busy week in my Vise to Squeeze Shop, if I decide to open it. (-;
...disregard ut, it's only ineedastoch fury because I blew his two days old new alias and reminded him that about 2-3 weeks ago he made announcement on MB about unloading his entire position in Nokia. Now u can better understand his frustration, desperation even madness. Poor INS, all I can do to help is to have him come to my Vise shop on Monday morning for a good squeeze to get an instant relief. I promised him to use on him a brand new hydraulic press. Only if I decide to open my Vshop on Monday, not sure yet.
Above message is example #2 where he abbreviates the letters INS for aka Ineedastock. Once again, that screen name is not reference anywhere else in thread an was introduced by screen user name zutko118. There is nothing in the thread that would require him to state that screen name except for the purpose of harrassment. This is being submitted as a reference after Zutko 118 threatened another screen name but referenced Ineedastock in his threat. This reply is for records only noted on 09/15/2013 at 15:10 hours.
Hope so. Im very very long here.... Quite worried about the fiscal debate though with equities right near the highs. wont take much to cause a selloff. but the fundamentals for euro area telecom seem much improved for 2014 and it apeears that story is gaining traction. Lot of potential upside to go for Nok, Alu.
cal, NOK is a safe haven thanks to the deal. In my estimation, now for sure very undervalued. Don't think there's a better long stock to be in to weather any downturn. In fact, I think when the financial media is spooking everyone about the upcoming reasons to fear a downturn that the market will actually do the exact opposite. As to risk tolerance, everyone has their own measure and will need to be self-referential in the consideration to buy or sell.
Sentiment: Strong Buy


text:
Mexico On The Square
Underneath its red awning, Mexico On The Square's large front window bears the words Tacos, Burritos, and Tortas in thick block letters. Inside, the menu shows much of the same: classic Mexican dishes with a foundation of thick, housemade tortillas that envelop savory fillings, such as a carnitas sope's deep-fried pork, refried beans, and pico de gallo. The menu also includes burritos, tortas, and picaditas packed with savory steak, shredded chicken, or veggies. American favorites such as burgers and hoagies offer more familiar bites, and in the morning, omelets and egg platters provide pleasant wake-up calls that are the culinary equivalent of a rooster's soulful saxophone licks.
"They should clean the place up a little, because it's kind of off-putting. But the food is great."
Rittenhouse Square
1511 Pine St.
Philadelphia, Pennsylvania 19102
215-732-1907Get Directions


text:
Wheelchair Accessible Vehicles & Cars
Wheelchair accessible vehicles can make a huge difference to your lifestyle. Literally thousands of disabled people have benefited from the chance to have their own wheelchair vehicle, offering new freedom and independence to individuals and their families.
Wheelchair Accessible Vehicles for Sale – UK & Ireland
Allied Mobility is the leading manufacturer and supplier of wheelchair accessible vehicles for the UK and Ireland, supplying direct to more than 3,000 customers every year. Disabled people, of course, each have different different needs, which is why we have developed a wide range of mobility adaptations and wheelchair accessible vehicles for sale and hire.
Working closely with leading manufacturers including Peugeot, Citroen, Volkswagen, Fiat and Ford, our engineering design team aims to create new and innovative ways to make wheelchair accessible transport as easy as possible. With large production volumes we're also able to deliver top-quality wheelchair accessible cars at highly competitive prices – with Allied Mobility, owning your own wheelchair accessible vehicle doesn't have to cost the earth.
Wheelchair Vehicles – Home Demonstrations
It is so important to make sure that wheelchair accessible vehicle conversions contain the right options to suit your needs. That's why Allied Mobility has a network of Mobility Consultants offering home demonstrations of our wheelchair vehicles throughout the UK and Ireland.
Wherever you live, we'll talk to you to discuss your needs then bring the wheelchair access vehicles that interest you most to your home, so that you can be sure you've made the right choice before you buy.
Wheelchair Adapted Vehicles – Safety and Quality
Our wheelchair accessible vehicles are all designed, tested and manufactured to meet stringent quality and safety standards. Each of our wheelchair adapted vehicles has been designed with accessibility in mind. From lowered floors and used-friendly wheelchair restraints to innovative, lightweight access ramps, buying from Allied Mobility means you can enjoy the reassurance of dealing with the UK's leading specialist in wheelchair accessible vehicle conversions.
Wheelchair Accessible Vehicles – Customer Recommendations
Don't just take our word for it! Click customer stories here to find out more about some of the thousands of people who enjoy a wheelchair accessible vehicle from Allied Mobility.


text:
President Obama to travel to Kenya in July
Obama will travel to the Global Entrepreneurship Summit, the White House announced.
Yelp CEO rips Indiana 'religious freedom' law
He believes these laws "set a terrible precedent" that will harm states' economies.
Starbucks wants its baristas to talk about race in America
"We at Starbucks should be willing to discuss these issues in America," Schultz said.
Ex-energy official's $1.7 million gig draws fire
Critics in Congress question Dan Poneman's new role as CEO of Centrus, a company with a history of relying on favors from Washington.
Jeb Bush selling off private business stakes
A Bush spokesman said he is "reviewing his private sector commitments as he contemplates a run."
Fraudsters target pot stocks
Publicly traded cannabis businesses are a new vehicle for scammers.
The woman behind the Fortune 50
Fortune's senior editor at large fell into her specialty three decades ago.
Buffett stands by Burger King deal
The billionaire investor says the purchase of Canada's Tim Horton's is not a tax dodge.
Employer insurance levels steady
It's only year one of Obamacare, and the findings don't quell the debate about businesses.
Apple event charges up D.C. Twitter
Pundits in the Beltway react to the new devices released today by the tech giant.
Amid worries, Apple unveils products
Experts say there are real questions about how information regarding your personal health is stored.
Chick-fil-A founder dies at 93
His religious views helped win Cathy and his family loyal following from conservative customers.
Google gets into game of drones
They invest in unmanned aerial vehicles and join the likes of Amazon and Facebook.
Plouffe's campaign: Steer to victory
He will likely play a role in transforming Uber.
W.H. meets big biz on immigration
Representatives for high-tech, agriculture and construction firms put forward a range of reforms.
Paul hits Obamas on minimum wage
The Kentucky Republican says the president supports limiting innovative companies.
Microsoft to cut up to 18,000 jobs
The news sends shares up 3 percent in premarket trading.
Women make gains in Maryland board rooms
Network 2000 Inc.'s current goal for Maryland is to have 20 percent of board seats filled by women by the year 2020.
Ex-Im's fate uncertain on Hill
The move throws into further jeopardy a program seen as key to American businesses.
Obama meet hits Dem talking points
The president aims to address policies that alleviate problems faced by working families.


text:
Black Saddle: Season 1, Episode 10
Client: Steele (21 Mar. 1959)
TV Episode | | Western
(awaiting 5 votes)
Reviews: 1 user
Clay agrees to take on the case of Bill Steele, one of a trio being held in jail by Marshal Scott on robbery charges.
Director:Francis D. Lyon
Writers:Hal Hudson (creator), John McGreevey (creator), 1 more credit »
Stars:Peter Breck, Russell Johnson, Anna-Lisa | See full cast and crew »


text:
Mx2 (Mission Minded) San Francisco, is a trip that Aftershock middle school students take during the summer. It was a great trip and God did great thing in and through these students.
Loading more stuff…
Hmm…it looks like things are taking a while to load. Try again?
TM + © 2015 Vimeo, LLC. All rights reserved.
Made with k in NYC.
Your content preferences apply to any Vimeo videos you view. Learn more about content preferences in our FAQ.


text:
We use cookies to optimise your experience on Travelzoo. By using our website, you are agreeing to allow this. For more information, see our Privacy & Cookie Policy »
Not valid on Saturdays, holidays or holiday weekends. Appointments are required; subject to availability. Limit 1 voucher per person; additional may be purchased as gifts. An automatic 18% gratuity charge will be added to the full value of the treatments.See all terms
M – F, 9am – 9pm ET
|Enjoyed Most
|Other Comments
The massage was incredible. I had Michael, and he was very knowledgeable and gave me tips for lower back pain and all my aches and pains. The facial was also very good.
This is a family hotel. So there were lots of screaming, splashing, diving-into-the-shallow-end kids playing in the pool the whole time we were there. Their parents were nearby, but I would hardly say they were supervising. Mainly because it took the father 35 minutes to realize his "princess" of a daughter dragged a patio chair into the pool and was playing on it. The pool area could really benefit from more hotel management.
|Mar 23, 2015 Stacey CA, United States
Was able to use the cabanas at the pool at no additional cost. Also liked having the choice to use the coed relaxation room or ladies only relaxation room.
Went with a girlfriend and the time just flew by. Very relaxing.
|Mar 20, 2015 Norma CA, United States
The massage was great! The steam room and the hot tea was enjoyable as well.
|Mar 11, 2015 Jan California, United States
Massage, access to pool, hot tub, and steam room. Enjoyed the lounge areas as well.
|Mar 10, 2015 Michelle CA, United States
Great service
|Mar 05, 2015 Margaret CA, United States
We are now transferring you...
We are now directing you to {0} where you can purchase today's Travelzoo Local Deal directly.
You've got deals!
Get the best travel and local deals delivered to your inbox for FREE!
This little detail makes a big difference in showing you deals that work for you.
Check your inbox. Confirm your email and we'll get you off and running.
Welcome back!


text:
Saracens will play Gloucester at Allianz Park on Saturday 11th October, kick off 15h00.
Season Tickets:
Season tickets are available from £250 Adults (£20.83 per month 0% Direct Debit) or £100 for Under 24s (£8.33 per month) or £165 for Over 65s (£13.75 per month). For more information call Supporter Services on 0203 675 7200.
Ticket Information and Match Day Prices:
Tickets can be purchased using the interactive map below, by clicking on the best available tab, or by calling our ticket office on 0844 847 1876.
Share this link with friends!
Event is no longer in event list


text:
Community Calendar
View by subject• Auditions
• Author Visits
• Book Clubs
• Careers
• Civic Meetings
• Class Reunions
• Classes
• Clubs
• Concerts
• Events
• Exhibits
• Film
• Fundraisers
• Health
• Holiday
• Kids & Teens
• Lectures/Workshops
• Live Music
• Miscellaneous
• On Stage
• Political Notes
• Recreation
• Scholarships
• Seniors
• Spiritual
• Sports
• Support Groups
• Volunteering
EventsNo Foolin' The Arts Mean Business
Randy Cohen, Vice President of Research, Americans for the Arts, will speak on the economic impact of the arts on communities and tourism at 6 p.m. on Wednesday, April 1 at the Bankhead Theater in Livermore. A reception with refreshments will be at 5 p.m. Contact 846-8910 or firstname.lastname@example.com. Bankhead Theater, 2400 First St., Livermore.
EventsGirls Night Out Networking Trade Show
GNON and Berkshire Hathaway Real Estate will host their 10th Annual Trade Show from 5-7 p.m. on Wednesday, April 1 in downtown Pleasanton. All Bay Area women are welcome. Shop with over 14 vendors, get raffle prizes and eat great food. Cost is $10 for members, $15 for non-members. RSVP and prepay required. Call 487-4748 or go to http://www.gnon.org/rsvp.html. , 4725 First St., Ste 150, Pleasanton.
ExhibitsFirefighter Homage and Mt. Diablo 2014 Vistas
Oil painter Vincent Liu exhibits a series of works depicting firefighters. Concurrently, watercolor artist Robin Purcell of Danville exhibits a collection of paintings featuring Mt. Diablo after the 2014 wildfires. Exhibit runs Feb. 25-April 4 at the Firehouse Arts Center. Contact the gallery director at 931-4849 or firstname.lastname@example.com. Firehouse Arts Center, 4444 Railroad Ave., Pleasanton.
SportsSoccer Training for Women
This fun class is designed to build your skill set and increase overall fitness. Classes are held from 6:30-7:30 p.m. on Fridays at Ken Mercer Sports Park, beginning April 10. Call 931-3437 or go to www.pleasantonfun.com. Ken Mercer Sports Park, 5800 Parkside Drive, Pleasanton.
VolunteeringOn-Call Volunteers Needed
The Pleasanton Senior Center is seeking on-call volunteers to work the Center's Front Receptionist Desk and VIP Travel Desk. Duties include answering phones, scheduling appointments, fielding information and referral questions. Shifts and times vary. For more information, call 931-5369. Pleasanton Senior Center, 5353 Sunol Blvd., Pleasanton.
SeniorsDessert Dance
Dance the night away from 7-9 p.m. on Friday, April 10 at the Pleasanton Senior Center. Enjoy dancing or listening to all of your ballroom favorites. Light refreshments will be served. Tickets are $7 for residents, $8 for non-residents. Register at the Senior Center Front Desk or pay at the door. Pleasanton Senior Center, 5353 Sunol Blvd., Pleasanton.
Exhibits'Order and Chaos'
The group of 7 South Bay artists known as "The Abstract 7" joins sculptural artist Melissa Woodburn in an interesting new exhibition with a wide variety of media, open March 7-April 11 at the Harrington Gallery at the Firehouse Arts Center. A free opening reception with the artists will be 7-9 p.m. on Wednesday, March 11. Call 931-4855. Harrington Art Gallery, Firehouse Arts Center, 4444 Railroad Ave., Pleasanton.
SeniorsChallenging Relationships with Adult Children
The Senior Support Program of the Tri-Valley presents an eight week discussion group covering living with adult children, distant or disengaged relationships, overly dependent adult children, and more, from 3-4:30 p.m. Wednesdays starting March 4 at the Pleasanton Senior Center. Call 931-7925 to register or for information. Pleasanton Senior Center, 5353 Sunol Blvd., Pleasanton.
FundraisersSleep Train's Shoe Drive for Foster Kids
Sleep Train is hosting its Shoe Drive to ensure foster kids have properly fitting, comfortable shoes and to support foster parents, now through April 26. Foster kids often arrive in new homes with just the clothes on their backs, and while foster families receive funding, it may not be enough. Call (800) 378-2337 or go to http://www.sleeptrain.com/. Sleep Train Mattress Centers, 6050 Johnson Drive, Ste C, Pleasanton.
EventsLivermore-Pleasanton Fire Department Community Emergency Response Team (CERT) Training
The Livermore-Pleasanton Fire Department will offer Community Emergency Response Team (CERT) Training from 6-9:30 p.m. on Monday evenings from March 30-April 27, and from 9 a.m.-4 p.m. on Saturday, April 18. Learn about basic disaster preparedness and beyond. Registration required, space limited. Contact 454-2361 or email@example.com. , , Pleasanton.
ExhibitsPleasanton Art League Imagination Expressed
The Pleasanton Art League's exhibit "Imagination Expressed 2015" runs now-May 3 at Museum on Main, 603 Main St. Come and see the latest creations from the vibrant art community of Pleasanton and the Valley. Contact email@example.com. Museum on Main, 603 Main St., Pleasanton.
ExhibitsMushrooms: Keys to the Kingdom Fungi Exhibit
"Mushrooms: Keys to the Kingdom Fungi" explores the lives and environmental roles of fungi featuring photographs by John Whittlesey and Jennifer Jewell, and will be at Alviso Adobe Community Park from March 22-May 17. This exhibit focuses on the ecology of mushroom-producing fungi. Call 931-3479 or go to www.pleasantonadobe.com. Alviso Adobe Community Park, 3465 Old Foothill Road, Pleasanton.
Support GroupsHappiness and Better Relationships
Learn how to have more fulfilling relationships with your partner, spouse and children; how to be more effective at work; and how to replace anger with peace and confidence. From 6:30-8:30 p.m. Wednesdays at Unity of Tri-Valley Office, 7567 Amador Valley Blvd. #120, Dublin. Cost is $5. Check online schedule at www.meetup.com/Tri-Valley-Real-Love-Group/ before coming. Unity of Tri-Valley Office, 7567 Amador Valley Blvd. #120, Dublin.
ClubsPleasanton Mothers Club
The mission of the Pleasanton Mothers Club is to provide a social, supportive, and educational outlet for moms and their families in the local community. They offer a variety of activities, children's playgroups, special interest groups, and more. For information visit pleasantonmothersclub.org. Contact email@example.com.
Support GroupsPleasanton Military Families Support Group
Formed in 2003 this group provides support and comfort to the Pleasanton families whose loved ones are deployed in the combat zones of Afghanistan and Iraq. The group has monthly meetings and other events such as "pack outs" of comfort and care items for deployed members of the armed forces. The group also sponsors the Yellow Streamer program on Main Street where streamers are displayed with the name, rank and branch of service of Pleasanton military personnel. Learn more at www.pleasantonmilitaryfamilies.org.
Civic MeetingsHuman Services Commission
The Human Services Commission meets at 7 p.m. on the first Wednesday of the month at City Council Chamber, 200 Old Bernal Ave. City Council Chamber, 200 Old Bernal Ave., Pleasanton.
ClassesFree Counseling Job Search Skills and Resume Writing
Get a free consultation with an experienced Employment Recruiter on select Wednesday evenings, Thursday afternoons and Saturday afternoons for a half or full hour at the Pleasanton Library. Receive help with resume writing, employment web sites, search for a job on the web, and get help with online applications. Make a 20-minute appointment at the Reference Desk by calling 931-3400, ext. 4. Pleasanton Library, 400 Old Bernal Ave., Pleasanton.
ClubsSenior Mens Social Club
Sons In Retirement (SIR) is a social club for senior men. In addition to friendships, members can participate in interest groups like golf, bridge, travel, photography, etc. We meet for lunch first Thursday of the month at San Ramon Golf Club. Open to retirees in the Tri-Valley. Contact Rich Osborne at 785-3549 or firstname.lastname@example.com. Go to branch34.sirinc2.org. San Ramon Golf Club, 9430 Fircrest Road, San Ramon.
SeniorsPeddler Shoppe at the Senior Center
The Peddler Shoppe in the lobby of the Pleasanton Senior Center, 5353 Sunol Blvd., offers the handmade wares of talented local senior artisans. It's a great place to buy gifts. The Shoppe is staffed by volunteers and is open to the public 10 a.m.-4 p.m. Monday through Friday. Go to www.pleasantonpeddlershoppe.com. Pleasanton Senior Center, 5353 Sunol Blvd., Pleasanton.
SeniorsDublin Senior Center Foundation Meeting
The Dublin Senior Center Foundation meets at 9 a.m. on the first Wednesday of every month, at the Dublin Senior Center, 7600 Amador Valley Blvd. Call 556-4511. Dublin Senior Center, 7600 Amador Valley Blvd., Dublin.
ClubsPleasanton Newcomers Club
This club is a great way for new and established residents to make new friends. It meets for coffee on the first Wednesday of every month and for lunch on the second Wednesday of every month. The group has activities like hiking, walking, Bunco and more. Visit www.pleasantonnewcomers.com. Contact email@example.com or 215-8405.
SeniorsComputer Classes for Seniors
Pleasanton Public Library hosts Computer Classes for Seniors including Beginning Internet on the first Wednesday and Thursday of every month; Beginning E-mail on the second Wednesday and Thursday of every month; Open Practice on the third Wednesday and Thursday of every month; Advanced E-mail on the fourth Wednesday and Thursday of every month, at the Adult Computer Area in the library, 400 Old Bernal Ave. Computer classes are designed for mature adults. Registration is required; call 931-3400. Pleasanton Library, 400 Old Bernal Ave., Pleasanton.
MiscellaneousFriends of the Library Online Book Sale
Did you know you could buy books from the Friends of the Library at Amazon.com? The Friends have a year round magazine and paperback book sale in the library and have two major book sales a year. To buy books, visit www.amazon.com/shops/ptwnfriends or call Nancy Bering at 462-4368.
SeniorsDublin Senior Center Dance Classes
Seniors are invited to a Beginning Latin Line Date from 1-2:15 p.m. Tuesdays; cost is $12 for four classes per month or $15 for five classes per month. Beginning line dance from 10:25-11:25 a.m. Thursdays, cost is $1.25 per class; beginning-intermediate line dancing from 10:15-11:15 a.m., Tuesdays, cost is $2 drop-in, or from 10:30 a.m.-noon Saturdays, cost is $3 drop-in; easy and intermediate line dance from 2-4 p.m. Thursdays, cost is $3 per class; intermediate line dance from 10:45-11:45 a.m. Fridays, cost is $1.25 per class; advanced line dancing from 12:30-2:30 p.m. Mondays, cost is $3 per class; or social line dance from 10:15-11:15 a.m. Tuesdays, cost is $2 for drop-in or $6 for four classes per month or $7.50 for five classes per month; all at the Dublin Senior Center, 7600 Amador Valley Blvd. Call 556-4511. Dublin Senior Center, 7600 Amador Valley Blvd., Dublin.
SeniorsDublin Senior Center Programs
Dublin Senior Center offers different programs and activities including "ESL" which helps seniors practice their English conversational skills, a Reading Group that meets monthly to discuss new books and a Needle Arts Group that enjoys quilting, sewing and knitting. Fees vary for each activity. For a complete list of activities, contact 556-4511 or firstname.lastname@example.com. Dublin Senior Center, 7600 Amador Valley Blvd., Dublin.
SeniorsDublin Senior Center Mini-Library
The Dublin Senior Center Mini-Library is open 9 a.m.-4 p.m., Monday-Friday at the senior center, 7600 Amador Valley Blvd., Dublin. The library gladly accepts donations of like-new used books published in the last five years, puzzles, magazines within three months of distribution, and videotapes. Unused books are donated to Friends of the Dublin Library. Bring donations to the office for processing. Call 556-4511. Dublin Senior Center, 7600 Amador Valley Blvd., Dublin.
EventsFarmers Market
Visit the Pleasanton Farmers Market from 9 a.m.-1 p.m. every Saturday, on East Angela Street between Main and First streets. The Farmers Market is open every Saturday, year-round, rain or shine, to provide the season's freshest fruits and vegetables, sold by the very farmers that planted, nurtured and harvested the crop. Downtown Pleasanton, E. Angela St., Pleasanton.


text:
Paula M Vertino
Emory University, Atlanta, Georgia, United States
Are you Paula M Vertino?
Claim your profile
Total impact
Available from: Scott Newman
Integrated Analysis of Whole-Genome Paired-End and Mate-Pair Sequencing Data for Identifying Genomic Structural Variations in Multiple Myeloma
Rendong Yang
Li Chen
Scott Newman
Khanjan Gandhi
Gregory Doho
Carlos S Moreno
, Paula M Vertino,
Leon Bernal-Mizarchi
Sagar Lonial
Lawrence H Boise
Michael Rossi
Jeanne Kowalski
Zhaohui S Qin
[Show abstract]
[Hide abstract]
We present a pipeline to perform integrative analysis of mate-pair (MP) and paired-end (PE) genomic DNA sequencing data. Our pipeline detects structural variations (SVs) by taking aligned sequencing read pairs as input and classifying these reads into properly paired and discordantly paired categories based on their orientation and inferred insert sizes. Recurrent SV was identified from the discordant read pairs. Our pipeline takes into account genomic annotation and genome repetitive element information to increase detection specificity. Application of our pipeline to whole-genome MP and PE sequencing data from three multiple myeloma cell lines (KMS11, MM.1S, and RPMI8226) recovered known SVs, such as heterozygous TRAF3 deletion, as well as a novel experimentally validated SPI1 - ZNF287 inter-chromosomal rearrangement in the RPMI8226 cell line.
Cancer informatics 09/2014; 13(Suppl 2):49-53. DOI:10.4137/CIN.S13783
Available from: Seth A. Brodie
Aberrant Promoter Methylation of Caveolin-1 Is Associated with Favorable Response to Taxane-Platinum Combination Chemotherapy in Advanced NSCLC
Seth A Brodie
Courtney Lombardo
Ge Li
Jeanne Kowalski
Khanjan Gandhi
Shaojin You
Fadlo R Khuri
Adam Marcus
, Paula M Vertino,
Johann C Brandes
[Show abstract]
[Hide abstract]
Aberrant promoter DNA methylation can serve as a predictive biomarker for improved clinical responses to certain chemotherapeutics. One of the major advantages of methylation biomarkers is the ease of detection and clinical application. In order to identify methylation biomarkers predictive of a response to a taxane-platinum based chemotherapy regimen in advanced NSCLC we performed an unbiased methylation analysis of 1,536 CpG dinucleotides in cancer-associated gene loci and correlated results with clinical outcomes.
PLoS ONE 09/2014; 9(9):e107124. DOI:10.1371/journal.pone.0107124
· 3.53 Impact Factor
Available from: Yanni Lin
CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences
Yanni Lin
Thomas J Cradick
Matthew T Brown
Harshavardhan Deshmukh
Piyush Ranjan
Neha Sarode
Brian M Wile
, Paula M Vertino,
Frank J Stewart
Gang Bao
[Show abstract]
[Hide abstract]
CRISPR/Cas9 systems are a versatile tool for genome editing due to the highly efficient targeting of DNA sequences complementary to their RNA guide strands. However, it has been shown that RNA-guided Cas9 nuclease cleaves genomic DNA sequences containing mismatches to the guide strand. A better understanding of the CRISPR/Cas9 specificity is needed to minimize off-target cleavage in large mammalian genomes. Here we show that genomic sites could be cleaved by CRISPR/Cas9 systems when DNA sequences contain insertions ('DNA bulge') or deletions ('RNA bulge') compared to the RNA guide strand, and Cas9 nickases used for paired nicking can also tolerate bulges in one of the guide strands. Variants of single-guide RNAs (sgRNAs) for four endogenous loci were used as model systems, and their cleavage activities were quantified at different positions with 1- to 5-bp bulges. We further investigated 114 putative genomic off-target loci of 27 different sgRNAs and confirmed 15 off-target sites, each harboring a single-base bulge and one to three mismatches to the guide strand. Our results strongly indicate the need to perform comprehensive off-target analysis related to DNA and sgRNA bulges in addition to base mismatches, and suggest specific guidelines for reducing potential off-target cleavage.
Nucleic Acids Research 05/2014; 42(11). DOI:10.1093/nar/gku402
· 8.81 Impact Factor
Epigenetic Changes Associated with Inflammation in Breast Cancer Patients Treated with Chemotherapy
Alicia K. Smith
Karen N. Conneely
Thaddeus W.W. Pace
Donna Mister
Jennifer C. Felger
Varun Kilaru
Mary J. Akel
, Paula M. Vertino,
Andrew H. Miller
Mylin A. Torres
[Show abstract]
[Hide abstract]
Inflammation has been associated with fatigue during and after various types of breast cancer treatments. We examined whether prior chemotherapy was associated with DNA methylation patterns that could explain persisting inflammation and/or fatigue in women treated for breast cancer. Prior to breast radiation therapy, DNA was extracted from peripheral blood mononuclear cells (PBMCs) of 61 Stage 0-IIIA breast cancer patients who had received partial mastectomy with or without chemotherapy. DNA methylation was assessed at >485,000 CpG sites across the genome along with fatigue and plasma inflammatory markers previously associated with fatigue. Compared to non-chemotherapy-treated women, women who had received chemotherapy exhibited significantly decreased methylation at eight CpG sites (p<1.03x10-7) including four in exon 11 of transmembrane protein 49 (TMEM49), which demonstrated the largest decreases in methylation. Lower methylation at each identified CpG site was associated with increased plasma soluble tumor necrosis factor receptor 2 (sTNFR2) and interleukin (IL)-6 and mediated the relationship between chemotherapy and increases in these inflammatory biomarkers adjusting for multiple clinical and treatment characteristics. sTNFR2, but not CpG methylation status, was correlated with fatigue. Six months after breast radiation therapy, DNA methylation, inflammatory biomarkers and fatigue assessments were repeated in a subset of subjects (N=39). Reduced methylation in 4 of the 8 identified CpG sites was still observed in chemotherapy versus non-chemotherapy-treated patients, albeit with some decay indicating the dynamic and potentially reversible nature of the changes. Reduced methylation in these 4 CpG sites also continued to correlate with either increased sTNFR2 or IL-6, but not fatigue. In conclusion, prior chemotherapy treatment was associated with decreased methylation of CpG sites in DNA from PBMCs of breast cancer patients, which correlated with increased inflammatory markers prior to and 6 months after radiation therapy. Persisting epigenetic changes secondary to chemotherapy may be one factor that contributes to inflammation and its consequences including cancer-related fatigue in vulnerable breast cancer patients.
Brain Behavior and Immunity 05/2014; DOI:10.1016/j.bbi.2014.02.010
· 6.13 Impact Factor
Dual role of PR-SET7-mediated H4K20me1 in the local regulation of RNA polymerase II promoter-proximal pausing.
Priya Kapoor-Vazirani
, Paula M Vertino
[Show abstract]
[Hide abstract]
RNA polymerase II (Pol II) promoter-proximal pausing plays a critical role in post-initiation transcriptional regulation at many metazoan genes. We recently showed that histone H4 lysine 16 acetylation (H4K16Ac), mediated by the MSL complex, facilitates the release of paused Pol II. In contrast, H4 lysine 20 trimethylation (H4K20me3), mediated by SUV420H2, enforces Pol II pausing by inhibiting MSL recruitment. However, how H4K16Ac and H4K20me3 are locally regulated in Pol II pausing remains unclear. Here, we demonstrate that PR-SET7/SETD8, which monomethylates histone H4 lysine 20 (H4K20me1), controls H4K16Ac and H4K20me3, and in so doing regulates Pol II pausing dynamics. PR-SET7-mediated H4K20me1 is necessary for the recruitment of the MSL complex, subsequent H4K16Ac and release of Pol II into active elongation. Although dispensable for SUV420H2 recruitment, PR-SET7-mediated H4K20me1 is required for H4K20me3. Whereas depletion of SUV420H2 is sufficient to deplete H4K20me3 and relieve an H4K20me3-induced pause, pausing is maintained in the absence of PR-SET7 despite H4K20me3 depletion due to an inability to recruit the MSL complex in the absence of H4K20me1. These findings highlight the requirement for PR-SET7 and H4K20me1 in establishing both the H4K16Ac and H4K20me3 marks and points to a dual role in the local regulation of Pol II pausing.
Journal of Biological Chemistry 01/2014; DOI:10.1074/jbc.M113.520783
· 4.60 Impact Factor
Class I HDACs Are Mediators of Smoke Carcinogen-Induced Stabilization of DNMT1 and Serve as Promising Targets for Chemoprevention of Lung Cancer
Seth A Brodie
Ge Li
Adam El-Kommos
Hyunseok Kang
Suresh S Ramalingam
Madhusmita Behera
Khanjan Gandhi
Jeanne Kowalski
Gabriel L Sica
Fadlo R Khuri
, Paula M Vertino,
Johann C Brandes
[Show abstract]
[Hide abstract]
DNA methylation is an early event in bronchial carcinogenesis and increased DNA methyltransferase (DNMT)1 protein expression is a crucial step in the oncogenic transformation of epithelia. Here, we investigate the role of class I histone deacetylases (HDACs) 1-3 in the stabilization of DNMT1 protein and as a potential therapeutic target for lung cancer chemoprevention. Long-term exposure of immortalized bronchial epithelial cells (HBEC-3KT) to low doses of tobacco-related carcinogens led to oncogenic transformation, increased HDAC expression, cell cycle independent increased DNMT1 stability and DNA hypermethylation. Overexpression of HDACs was associated with increased DNMT1 stability and knockdown of HDACs reduced DNMT1 protein levels and induced DNMT1 acetylation. This suggests a causal relationship among increased class I HDACs levels, upregulation of DNMT1 protein, and subsequent promoter hypermethylation. Targeting of class I HDACs with valproic acid (VPA) was associated with reduced HDAC expression and a profound reduction of DNMT1 protein level. Treatment of transformed bronchial epithelial cells with VPA resulted in reduced colony formation, demethylation of the aberrantly methylated SFRP2 promoter and de-repression of SFRP2 transcription. These data suggest that inhibition of HDAC activity may reverse or prevent carcinogen induced transformation. Finally, immunohistochemistry on human lung cancer specimens revealed a significant increase in DNMT1, HDAC1, HDAC2, and HDAC3 expression, supporting our hypotheses that class I HDACs are mediators of DNMT1 stability.
Cancer Prevention Research 01/2014; 7(3). DOI:10.1158/1940-6207.CAPR-13-0254
· 4.89 Impact Factor
Available from: Adam Marcus
Withania somnifera Root Extract Inhibits Mammary Cancer Metastasis and Epithelial to Mesenchymal Transition.
Zhen Yang
Anapatricia Garcia
Songli Xu
Doris R Powell
, Paula M Vertino,
Shivendra Singh
Adam I Marcus
[Show abstract]
[Hide abstract]
Though clinicians can predict which patients are at risk for developing metastases, traditional therapies often prove ineffective and metastatic disease is the primary cause of cancer patient death; therefore, there is a need to develop anti-metastatic therapies that can be administered over long durations to specifically inhibit the motility of cancer cells. Withaniasomnifera root extracts (WRE) have anti-proliferative activity and the active component, Withaferin A, inhibits the pro-metastatic protein, vimentin. Vimentin is an intermediate filament protein and is part of the epithelial to mesenchymal transition (EMT) program to promote metastasis. Here, we determined whether WRE standardized to Withaferin A (sWRE) possesses anti-metastatic activity and whether it inhibits cancer motility via inhibition of vimentin and the EMT program. Several formulations of sWRE were created to enrich for Withaferin A and a stock solution of sWRE in EtOH could recover over 90% of the Withaferin A found in the original extract powder. This sWRE formulation inhibited breast cancer cell motility and invasion at concentrations less than 1µM while having negligible cytotoxicity at this dose. sWRE treatment disrupted vimentin morphology in cell lines, confirming its vimentin inhibitory activity. To determine if sWRE inhibited EMT, TGF-β was used to induce EMT in MCF10A human mammary epithelial cells. In this case, sWRE prevented EMT induction and inhibited 3-D spheroid invasion. These studies were taken into a human xenograft and mouse mammary carcinoma model. In both models, sWRE and Withaferin A showed dose-dependent inhibition of tumor growth and metastatic lung nodule formation with minimal systemic toxicity. Taken together, these data support the hypothesis that low concentrations of sWRE inhibit cancer metastasis potentially through EMT inhibition. Moreover, these doses of sWRE have nearly no toxicity in normal mouse organs, suggesting the potential for clinical use of orally administered WRE capsules.
PLoS ONE 09/2013; 8(9):e75069. DOI:10.1371/journal.pone.0075069
· 3.53 Impact Factor
Available from: Adam Marcus
Dysregulation of mTOR activity through LKB1 inactivation
Wei Zhou
Adam I Marcus
, Paula M. Vertino
[Show abstract]
[Hide abstract]
Mammalian target of Rapamycin (mTOR) is aberrant activated in many cancer types, and two Rapamycin derivatives are currently approved by FDA for the treatment of renal cell carcinoma. Mechanistically, mTOR is hyperactivated in human cancers either due to the genetic activation of its upstream activating signaling pathways or the genetic inactivation of its negative regulators. The tumor suppressor liver kinase B1 (LKB1; also known as serine/threonine kinase 11, STK11) involves in cell polarity, cell detachment and adhesion, tumor metastasis, and energetic stress response. One of LKB1's key roles is to negatively regulate mTOR complex 1 (mTORC1) activity. This review summarizes the molecular basis of this negative interaction, and recent research progress in this area.
Ai zheng = Aizheng = Chinese journal of cancer 05/2013; 32(8). DOI:10.5732/cjc.013.10086
Available from: europepmc.org
A heterozygous IDH1R132H/WT mutation induces genome-wide alterations in DNA methylation.
Christopher G Duncan
Benjamin G Barwick
Genglin Jin
Carlo Rago
Priya Kapoor-Vazirani
Doris R Powell
Jen Tsan Chi
Darell D Bigner
, Paula M Vertino,
Hai Yan
[Show abstract]
[Hide abstract]
Monoallelic point mutations of the NADP(+)-dependent isocitrate dehydrogenases IDH1 and IDH2 occur frequently in gliomas, acute myeloid leukemias, and chondromas and display robust association with specific DNA hypermethylation signatures. Here we show that heterozygous expression of the IDH1(R132H) allele is sufficient to induce the genome-wide alterations in DNA methylation characteristic of these tumors. Using a gene targeting approach, we knocked-in a single copy of the most frequently observed IDH1 mutation, R132H, into a human cancer cell line and profiled changes in DNA methylation at over 27,000 CpG dinucleotides relative to wild-type parental cells. We find that IDH1(R132H/WT) mutation induces widespread alterations in DNA methylation, including hypermethylation of 2,010 and hypomethylation of 842 CpG loci. We demonstrate that many of these alterations are consistent with those observed in IDH1-mutant and G-CIMP+ primary gliomas and can segregate IDH wild-type and mutated tumors, as well as those exhibiting the G-CIMP phenotype in unsupervised analysis of two primary glioma cohorts. Further, we show that the direction of IDH1(R132H/WT)-mediated DNA methylation change is largely dependent upon pre-existing DNA methylation levels, resulting in depletion of moderately methylated loci. Additionally, whereas the levels of multiple histone H3 and H4 methylation modifications were globally increased, consistent with broad inhibition of histone demethylation, hypermethylation at H3K9 in particular accompanied locus-specific DNA hypermethylation at several genes downregulated in IDH1(R132H/WT) knock-in cells. These data provide insight on epigenetic alterations induced by IDH1 mutations and support a causal role for IDH1(R132H/WT) mutants in driving epigenetic instability in human cancer cells.
Genome Research 08/2012; DOI:10.1101/gr.132738.111
· 13.85 Impact Factor
R loops: lassoing DNA methylation at CpGi.
Paula M Vertino,
Paul A Wade
[Show abstract]
[Hide abstract]
In this issue of Molecular Cell, Ginno et al. (2012) describe unusual sequence features at promoter CpG islands that can lead to formation of persistent RNA-DNA hybrids (R loops), which are proposed to prevent genomic DNA methylation.
Molecular cell 03/2012; 45(6):708-9. DOI:10.1016/j.molcel.2012.03.014
· 14.46 Impact Factor
Available from: Yanqi Chang
Recognition and potential mechanisms for replication and erasure of cytosine hydroxymethylation.
Hideharu Hashimoto
Yiwei Liu
Anup K Upadhyay
Yanqi Chang
Shelley B Howerton
, Paula M Vertino,
Xing Zhang
Xiaodong Cheng
[Show abstract]
[Hide abstract]
Cytosine residues in mammalian DNA occur in at least three forms, cytosine (C), 5-methylcytosine (M; 5mC) and 5-hydroxymethylcytosine (H; 5hmC). During semi-conservative DNA replication, hemi-methylated (M/C) and hemi-hydroxymethylated (H/C) CpG dinucleotides are transiently generated, where only the parental strand is modified and the daughter strand contains native cytosine. Here, we explore the role of DNA methyltransferases (DNMT) and ten eleven translocation (Tet) proteins in perpetuating these states after replication, and the molecular basis of their recognition by methyl-CpG-binding domain (MBD) proteins. Using recombinant proteins and modified double-stranded deoxyoligonucleotides, we show that DNMT1 prefers a hemi-methylated (M/C) substrate (by a factor of >60) over hemi-hydroxymethylated (H/C) and unmodified (C/C) sites, whereas both DNMT3A and DNMT3B have approximately equal activity on all three substrates (C/C, M/C and H/C). Binding of MBD proteins to methylated DNA inhibited Tet1 activity, suggesting that MBD binding may also play a role in regulating the levels of 5hmC. All five MBD proteins generally have reduced binding affinity for 5hmC relative to 5mC in the fully modified context (H/M versus M/M), though their relative abilities to distinguish the two varied considerably. We further show that the deamination product of 5hmC could be excised by thymine DNA glycosylase and MBD4 glycosylases regardless of context.
Nucleic Acids Research 02/2012; 40(11):4841-9. DOI:10.1093/nar/gks155
· 8.81 Impact Factor
Available from: R. Donald Harvey
Withaferin A inhibits breast cancer invasion and metastasis at sub-cytotoxic doses by inducing vimentin disassembly and serine 56 phosphorylation
Jose T Thaiparambil
Laura Bender
Thota Ganesh
Erik Kline
Pritty Patel
Yuan Liu
Mourad Tighiouart
, Paula M Vertino,
R Donald Harvey
Anapatricia Garcia
Adam I Marcus
[Show abstract]
[Hide abstract]
Withaferin A (WFA) is purified from the plant Withania somnifera and inhibits the vimentin cytoskeleton. Vimentin overexpression in cancer correlates with metastatic disease, induction of epithelial to mesenchymal transition and reduced patient survival. As vimentin functions in cell motility, we wanted to test the hypothesis that WFA inhibits cancer metastasis by disrupting vimentin function. These data showed that WFA had weak cytotoxic and apoptotic activity at concentrations less than or equal to 500 nM, but retained potent anti-invasive activity at these low doses. Imaging of breast cancer cell lines revealed that WFA induces perinuclear vimentin accumulation followed by rapid vimentin depolymerization. A concomitant induction of vimentin ser56 phosphorylation was observed, which is consistent with vimentin disassembly. Structure activity relationships were established using a set of chemically modified WFA analogs and showed that the predicted vimentin-binding region of WFA is necessary to induce vimentin ser56 phosphorylation and for its anti-invasive activity. Pharmacokinetic studies in mice revealed that WFA reaches peak concentrations up to 2 μM in plasma with a half-life of 1.36 hr following a single 4 mg/kg dose. In a breast cancer metastasis mouse model, WFA showed dose-dependent inhibition of metastatic lung nodules and induced vimentin ser56 phosphorylation, with minimal toxicity to lung tissue. Based upon these studies, we conclude that WFA is a potent breast cancer anti-metastatic agent and the anti-metastatic activity of WFA is, at least in part, mediated through its effects on vimentin and vimentin ser56 phosphorylation.
International Journal of Cancer 12/2011; 129(11):2744-55. DOI:10.1002/ijc.25938
· 6.20 Impact Factor
Available from: Erwin G Van Meir
Overexpression of MBD2 in Glioblastoma Maintains Epigenetic Silencing and Inhibits the Antiangiogenic Function of the Tumor Suppressor Gene BAI1
Dan Zhu
Stephen B Hunter
, Paula M Vertino,
Erwin G Van Meir
[Show abstract]
[Hide abstract]
Brain angiogenesis inhibitor 1 (BAI1) is a putative G protein-coupled receptor with potent antiangiogenic and antitumorigenic properties that is mutated in certain cancers. BAI1 is expressed in normal human brain, but it is frequently silenced in glioblastoma multiforme. In this study, we show that this silencing event is regulated by overexpression of methyl-CpG-binding domain protein 2 (MBD2), a key mediator of epigenetic gene regulation, which binds to the hypermethylated BAI1 gene promoter. In glioma cells, treatment with the DNA demethylating agent 5-aza-2'-deoxycytidine (5-Aza-dC) was sufficient to reactivate BAI1 expression. Chromatin immunoprecipitation showed that MBD2 was enriched at the promoter of silenced BAI1 in glioma cells and that MBD2 binding was released by 5-Aza-dC treatment. RNA interference-mediated knockdown of MBD2 expression led to reactivation of BAI1 gene expression and restoration of BAI1 functional activity, as indicated by increased antiangiogenic activity in vitro and in vivo. Taken together, our results suggest that MBD2 overexpression during gliomagenesis may drive tumor growth by suppressing the antiangiogenic activity of a key tumor suppressor. These findings have therapeutic implications because inhibiting MBD2 could offer a strategy to reactivate BAI1 and suppress glioma pathobiology.
Cancer Research 07/2011; 71(17):5859-70. DOI:10.1158/0008-5472.CAN-11-1157
· 9.28 Impact Factor
Available from: ncbi.nlm.nih.gov
SUV420H2-mediated H4K20 trimethylation enforces RNA polymerase II promoter-proximal pausing by blocking hMOF-dependent H4K16 acetylation.
Priya Kapoor-Vazirani
Jacob D Kagey
, Paula M Vertino
[Show abstract]
[Hide abstract]
Many human genes exhibit evidence of initiated RNA polymerase II (Pol II) at their promoters, despite a lack of significant full-length transcript. Such genes exhibit promoter-proximal "pausing," wherein initiated Pol II accumulates just downstream of the transcription start site due to a rate-limiting step mediating the transition to elongation. The mechanisms that regulate the escape of Pol II from pausing and the relationship to chromatin structure remain incompletely understood. Recently, we showed that CpG island hypermethylation and epigenetic silencing of TMS1/ASC in human breast cancers are accompanied by a local shift from histone H4 lysine 16 acetylation (H4K16Ac) to H4 lysine 20 trimethylation (H4K20me3). Here, we show that hMOF-mediated H4K16Ac and SUV420H2-mediated H4K20me3 play opposing roles in the regulation of Pol II pausing. We found that H4K16Ac promoted the release of Pol II from pausing through the recruitment of BRD4 and pTEFb. Aberrant methylation of CpG island DNA blocked Pol II recruitment to gene promoters. Whereas the inhibition of DNA methylation allowed for the reassociation and initiation of Pol II at the TMS1 promoter, Pol II remained paused in the presence of H4K20me3. Combined inhibition of H4K20me3 and DNA methylation resulted in the rerecruitment of hMOF and subsequent H4K16Ac, release of Pol II into active elongation, and synergistic reactivation of TMS1 expression. Marking by H4K20me3 was not restricted to TMS1 but also occurred at other genes independently of DNA methylation, where it similarly imposed a block to Pol II promoter escape through a mechanism that involved the local inhibition of H4K16Ac. These data indicate that H4K20me3 invokes gene repression by antagonizing hMOF-mediated H4K16Ac and suggest that overcoming Pol II pausing might be a rate-limiting step in achieving tumor suppressor gene reactivation in cancer therapy.
Molecular and Cellular Biology 02/2011; 31(8):1594-609. DOI:10.1128/MCB.00524-10
· 5.04 Impact Factor
Available from: ncbi.nlm.nih.gov
Molecular coupling of DNA methylation and histone methylation.
Hideharu Hashimoto
, Paula M Vertino,
Xiaodong Cheng
[Show abstract]
[Hide abstract]
The combinatorial pattern of DNA and histone modifications constitutes an epigenetic 'code' that shapes gene-expression patterns by enabling or restricting the transcriptional potential of genomic domains. DNA methylation is associated with histone modifications, particularly the absence of histone H3 lysine 4 methylation (H3K4me0) and the presence of H3K9 methylation. This article focuses on three protein domains (ATRX-Dnmt3-Dnmt3L [ADD], Cys-X-X-Cys [CXXC] and the methyl-CpG-binding domain [MBD]) and the functional implications of domain architecture in the mechanisms linking histone methylation and DNA methylation in mammalian cells. The DNA methyltransferase DNMT3a and its accessory protein Dnmt 3L contain a H3K4me0-interacting ADD domain that links the DNA methylation reaction with unmodified H3K4. The H3K4 methyltransferase MLL1 contains a CpG-interacting CXXC domain that may couple the H3K4 methylation reaction to unmethylated DNA. Another H3K4 methyltransferase, SET1, although lacking an intrinsic CXXC domain, interacts directly with an accessory protein CFP1 that contains the same domain. The H3K9 methyltransferase SETDB1 contains a putative MBD that potentially links the H3K4 methylation reaction to methylated DNA or may do so through the interaction with the MBD containing protein MBD1. Finally, we consider the domain structure of the DNA methyltransferase DNMT1, its accessory protein UHRF1 and their associated proteins, and propose a mechanism by which DNA methylation and histone methylation may be coordinately maintained through mitotic cell division, allowing for the transmission of parental DNA and for the histone methylation patterns to be copied to newly replicated chromatin.
Epigenomics 10/2010; 2(5):657-69. DOI:10.2217/epi.10.44
· 2.43 Impact Factor
Available from: ncbi.nlm.nih.gov
Long-term stability of demethylation after transient exposure to 5-aza-2'-deoxycytidine correlates with sustained RNA polymerase II occupancy.
Jacob D Kagey
Priya Kapoor-Vazirani
Michael T McCabe
Doris R Powell
, Paula M Vertino
[Show abstract]
[Hide abstract]
DNA methyltransferase inhibitors are currently the standard of care for myelodysplastic syndrome and are in clinical trials for leukemias and solid tumors. However, the molecular basis underlying their activity remains poorly understood. Here, we studied the induction and long-term stability of gene reactivation at three methylated tumor suppressor loci in response to the DNA methyltransferase inhibitor 5-aza-2'-deoxycytidine (5-azaCdR) in human breast cancer cells. At the TMS1/ASC locus, treatment with 5-azaCdR resulted in partial DNA demethylation, the reengagement of RNA polymerase II (Pol II), and a shift from a repressive chromatin profile marked with H3K9me2 and H4K20me3 to an active profile enriched in H3ac and H3K4me2. Using a single-molecule approach coupling chromatin immunoprecipitation with bisulfite sequencing, we show that H3ac, H3K4me2, and Pol II selectively associated with the demethylated alleles, whereas H3K9me2 preferentially marked alleles resistant to demethylation. H4K20me3 was unaffected by DNA demethylation and associated with both unmethylated and methylated alleles. After drug removal, TMS1 underwent partial remethylation, yet a subset of alleles remained stably demethylated for over 3 months. These alleles remained selectively associated with H3K4me2, H3ac, and Pol II and correlated with a sustained low level of gene expression. TMS1 alleles reacquired H3K9me2 over time, and those alleles that became remethylated retained H3ac. In contrast, CDH1 and ESR1 were remethylated and completely silenced within approximately 1 week of drug removal, and failed to maintain stably unmethylated alleles. Our data suggest that the ability to maintain Pol II occupancy is a critical factor in the long-term stability of drug-induced CpG island demethylation.
Molecular Cancer Research 07/2010; 8(7):1048-59. DOI:10.1158/1541-7786.MCR-10-0189
· 4.35 Impact Factor
Available from: Ryan E. Mills
Natural mutagenesis of human genomes by endogenous retrotransposons.
Rebecca C Iskow
Michael T McCabe
Ryan E Mills
Spencer Torene
W Stephen Pittard
Andrew F Neuwald
Erwin G Van Meir
, Paula M Vertino,
Scott E Devine
[Show abstract]
[Hide abstract]
Two abundant classes of mobile elements, namely Alu and L1 elements, continue to generate new retrotransposon insertions in human genomes. Estimates suggest that these elements have generated millions of new germline insertions in individual human genomes worldwide. Unfortunately, current technologies are not capable of detecting most of these young insertions, and the true extent of germline mutagenesis by endogenous human retrotransposons has been difficult to examine. Here, we describe technologies for detecting these young retrotransposon insertions and demonstrate that such insertions indeed are abundant in human populations. We also found that new somatic L1 insertions occur at high frequencies in human lung cancer genomes. Genome-wide analysis suggests that altered DNA methylation may be responsible for the high levels of L1 mobilization observed in these tumors. Our data indicate that transposon-mediated mutagenesis is extensive in human genomes and is likely to have a major impact on human biology and diseases.
Cell 06/2010; 141(7):1253-61. DOI:10.1016/j.cell.2010.05.020
· 31.96 Impact Factor
Available from: Rana Bayakly
Age/Race Differences in HER2 Testing and in Incidence Rates for Breast Cancer Triple Subtypes A Population-Based Study and First Report
Mary Jo Lund
Ebonee N Butler
Brionna Y Hair
Kevin C Ward
Judy H Andrews
Gabriella Oprea-Ilies
A Rana Bayakly
Ruth M O'Regan
, Paula M Vertino,
J William Eley
[Show abstract]
[Hide abstract]
Although US year 2000 guidelines recommended characterizing breast cancers by human epidermal growth factor receptor 2 (HER2), national cancer registries do not collect HER2, rendering a population-based understanding of HER2 and clinical "triple subtypes" (estrogen receptor [ER] / progesterone receptor [PR] / HER2) largely unknown. We document the population-based prevalence of HER2 testing / status, triple subtypes and present the first report of subtype incidence rates. Medical records were searched for HER2 on 1842 metropolitan Atlanta females diagnosed with breast cancer during 2003-2004. HER2 testing/status and triple subtypes were analyzed by age, race/ethnicity, tumor factors, socioeconomic status, and treatment. Age-adjusted incidence rates were calculated. Over 90% of cases received HER2 testing: 12.6% were positive, 71.7% negative, and 15.7% unknown. HER2 testing compliance was significantly better for women who were younger, of Caucasian or African-American descent, or diagnosed with early stage disease. Incidence rates (per 100,000) were 21.1 for HER2+ tumors and 27.8 for triple-negative tumors, the latter differing by race (36.3 and 19.4 for black and white women, respectively). HER2 recommendations are not uniformly adhered to. Incidence rates for breast cancer triple subtypes differ by age/race. As biologic knowledge is translated into the clinical setting eg, HER2 as a biomarker, it will be incumbent upon national cancer registries to report this information. Incidence rates cautiously extrapolate to an annual burden of 3000 and 17,000 HER2+ tumors for black and white women, respectively, and triple-negative tumors among 5000 and 16,000 respectively. Testing, rate, and burden variations warrant population-based in-depth exploration and clinical translation.
Cancer 03/2010; 116(11):2549-59. DOI:10.1002/cncr.25016
· 4.90 Impact Factor
Available from: Wei Zhou
Homozygous deletion of the STK11/LKB1 locus and the generation of novel fusion transcripts in cervical cancer cells
Michael T McCabe
Doris R Powell
Wei Zhou
, Paula M Vertino
[Show abstract]
[Hide abstract]
The STK11/LKB1 gene encodes a ubiquitously expressed serine/threonine kinase that is mutated in multiple sporadic cancers including non-small cell lung carcinomas, pancreatic cancers, and melanomas. LKB1 plays a role in multiple cellular functions including cell growth, cell cycle progression, metabolism, cell polarity, and migration. To date, only a limited number of studies have assessed the status of LKB1 in cervical cancers. Herein, we investigate DNA methylation, DNA mutation, and transcription at the LKB1 locus in cervical cancer cell lines. We identified homozygous deletions of 25-85kb in the HeLa and SiHa cell lines. Deletion breakpoint analysis in HeLa cells revealed that the deletion resulted from an Alu-recombination-mediated deletion (ARMD) and generated a novel LKB1 fusion transcript driven by an uncharacterized CpG island promoter located approximately 11kb upstream of LKB1. Although the homozygous deletion in SiHa cells removes the entire LKB1 gene and portions of the neighboring genes SBNO2 and c19orf26, this deletion also generates a fusion transcript driven by the c19orf26 promoter and composed of both c19orf26 and SBNO2 sequences. Further analyses of public gene expression and mutation databases suggest that LKB1 and its neighboring genes are frequently dysregulated in primary cervical cancers. Thus, homozygous deletions affecting LKB1 in cervical cancers may generate multiple fusion transcripts involving LKB1, SBNO2, and c19orf26.
Cancer genetics and cytogenetics 03/2010; 197(2):130-41. DOI:10.1016/j.cancergencyto.2009.11.017
· 1.54 Impact Factor
Available from: Johann C Brandes
Cancer DNA Methylation: Molecular Mechanisms and Clinical Implications
Michael T McCabe
Johann C Brandes
, Paula M Vertino
[Show abstract]
[Hide abstract]
DNA methylation plays a crucial role in the regulation of gene expression and chromatin organization within normal eukaryotic cells. In cancer, however, global patterns of DNA methylation are altered with global hypomethylation of repeat-rich intergenic regions and hypermethylation of a subset of CpG-dense gene-associated regions (CpG islands). Extensive research has revealed the cellular machinery that catalyzes DNA methylation, as well as several large protein complexes that mediate the transcriptional repression of hypermethylated genes. However, research is only just beginning to uncover the molecular mechanisms underlying the origins of cancer-specific DNA methylation. Herein, we present several recent advances regarding these mechanisms and discuss the relationship between histone modifications (i.e., H3K4me2/3, H4K16Ac, H3K9me2/3, H3K27me3, H4K20me3), chromatin-modifying enzymes (G9a, EZH2, hMOF, SUV4-20H), and aberrant DNA methylation. Additionally, the role played by inflammation, DNA damage, and miRNAs in the etiology of aberrant DNA methylation is considered. Finally, we discuss the clinical implications of aberrant DNA methylation and the utility of methylated biomarkers in cancer diagnosis and management.
Clinical Cancer Research 07/2009; 15(12):3927-37. DOI:10.1158/1078-0432.CCR-08-2784
· 8.19 Impact Factor
Publication Stats
Total Impact Points
Top co-authors
View all
Wei Zhou
Emory University
Remove suggestion
Michael T McCabe
GlaxoSmithKline plc.
Remove suggestion
Dipali Sharma
Johns Hopkins Medicine
Remove suggestion
Adam Marcus
Emory University
Remove suggestion
Jean-Pierre Issa
Temple University
Remove suggestion
Top Journals
Journal of Biological Chemistry
Cancer Research
Cancer Research
Proceedings of the National Academy of Scienc...
Lung Cancer
Emory University
• Department of Radiation Oncology
• Winship Cancer Institute
Atlanta, Georgia, United States
Georgia Institute of Technology
Atlanta, Georgia, United States
Columbia University
Department of Genetics and Development
New York City, NY, United States
Johns Hopkins University
Baltimore, Maryland, United States
Developmental Biology
Cancer Research
Cell Biology
Molecular Biology
Clinical Trials
Browse more researchers
© 2008‐2015 researchgate.net. All rights reserved.
About us
Contact us
Sign up
Already a member?
Log in
Log in
Forgot password?
Keep me logged in
Log in with Linkedin
Log in with Facebook
Sign Up »
ResearchGate is the professional network for scientists and researchers. Join today, it's free!


text:
Florida Horsemen Oppose Furosemide Ban
Furosemide (Lasix) is diuretic that has long been abused by both humans and horses to mask other illegal drug use. ~ HfH From: The Blood-Horse In response to a statement from 25 prominent horsemen calling for a ban on the race-day use of furosemide (Salix, commonly called Lasix), the Florida Horsemen's Benevolent and Protective Association Full Article
New Connecticut Law Declares Horses Not Naturally Vicious
When the Connecticut Supreme Court ruled that horses were naturally vicious, the large population of horse owners there (and Connecticut does love it horses) rallied for justice on behalf ...Full Article
Protest Being Organized – Protect the Last of the West's Wild Horses
For those of you who live close or can travel to Riverton, Wyoming later this month – here is the address of the BLM Meeting: Little Theater (SC 109), ...Full Article
U.S. millionaire eager to protect agriculture
Protect agriculture?? Forrest Lucas makes it sound like the cattle industry is dying. The majority of cattle ranchers are not poor dirt farmers trying to get by for another ...Full Article
Dozens Of Horses Mysteriously Sick With Undetermined Condition (Video)
From: Los Angeles CBS PHELAN (CBSLA.com) — A mysterious condition has left dozens of horses sick and owners searching for answers the high desert. KCAL9's Tom Wait met with ...Full Article
Video – Gus Warr of the BLM explains Blawn Wash Round Up
The audio is not very good on this video – you may need to turn your speakers up all the way or put on ear phones. Gus Warr of ...Full Article
BLM To Re-educate Native Americans On How To Live With Nature
Keep in mind folks that this article is very purposefully tongue-in-cheek. Point made none-the-less. ~ HfH From: Personal Liberty Native Americans, infamous throughout human history for their notoriously scornful ...Full Article
BLM ships 1,770 wild horses out … without notifying the public
From: The PPJ Gazette By: Debbie Coffey BLM ships 1,770 wild horses out of Teterville Long Term Holding Pasture in Kansas (without notifying the public) Over the course of ...Full Article
Symbols of pioneer spirit they may be, but wild mustangs …
An excellent article if you are looking for basic information on the history of the wild horse preservation movement. Especially how the Free Roaming Wild Horse and Burro Act ...Full Article
BLM, local law enforcement tensions near breaking point in the West
If you remember, this all started back in March when the BLM restricted access to public land to remove Cliven Bundy's cattle which were illegally on the land. Bundy ...Full Article


text:
Lost? Site Map
39 recipes in
Christmas Treats
Cookbook by
I called this Christmas Treats because at Christmas time I bake these wonderful treats and give them as gifts. You can certainly bake them for your own family. I have personally tried all of these recipes and would not hesitate to include any of them in the "goodie" boxes I give away at Christmas.
Sort by: Newest | Rating | With Photo | Time to Make | Alphabetical
17 Reviews | By Mirj
Recipe #23933
Recipe #15059
Recipe #193285
Recipe #63375
Recipe #14914
Looking For More?
Free Weekly Newsletter


text:
Address:3208 N Loop 250 W Suite 900Midland, TX 79707
Hours of Operation:10:00 AM - 6:00 PM Sunday 8:00 AM - 10:00 PM Monday-Friday 8:00 AM - 6:00 PM Saturday
(432) 550-3689
In the Midland Marketplace at North Loop 250 West and Wadley Avenue in front of Kohl's
Need to find the perfect place for customized, affordable and
convenient Midland facials and massages? It's right in your own
backyard at Midland Massage Envy Spa.
With customized massages and Murad® Healthy Skin facials, you
can help relieve stress, decrease anxiety, increase energy and
improve your skin texture, tone and appearance. Our professional
massage therapists and estheticians will work with you to customize
your session just for you.
Always have a million things to do before the end of the day? No
problem. Consider Massage Envy Spa your Midland spa. Our
convenient late night and weekend hours make it easy to fit a
session into your busy schedule. Start your healthier lifestyle by
visiting your neighborhood Massage Envy Spa today for the best
Midland facials and massages around.
We are looking for Massage Therapists and Estheticians that are passionate about people. Apply today at www.MassageEnvyCareers.com
We are now hiring for the following positions:
Sign up to receive timely Massage Envy Spa Midland news and promotions.
Explore the benefits to unlocking a more healthy, energetic, and
stress free lifestyle.
Learn about all of the benefits
of massage therapy
Professionally educated massage therapists who meet all state
and local licensing requirements.


text:
SheKnows Media ‐ Food
© Copyright 2003-2015 SheKnows, LLC.
All Rights Reserved.
by Michele Borboa
Serves 10
Vegan granola bread Vegan banana bread Vegan recipes
You've got meal! Get daily recipe inspiration.
The menu button now contains all of the sections of our site.
And you'll see personalized content just for you whenever you click the My Feed .
SheKnows is making some changes!


text:
ESPN announced television network designations and start times for the 2014 SEC/Big 12 Challenge Presented by Sonic on Tuesday, where it was determined South Carolina's Dec. 6 contest versus Oklahoma State in Columbia will tip at Noon ET, and will be televised on ESPNU.
Click here for the original story.
Welcome to SECTalk.com
|Welcome to SECTalk.com -- The Home of 6 Straight National Titles!
You are currently accessing our site as a guest which means you can't access all of our features such as social groups, sports betting, and many more. By joining our free community you will have access to all of these great features as well as to participating in our forums, contacting other members, and much more. Registration only takes a minute and SECTalk.com is absolutely free, so please join today!
If you have any problems registering or signing in, please contact us.
Latest Topics
Latest News
Top Bettors
Top High Fived
Tip Time Set For SEC/Big 12 Challenge Matchup Versus Oklahoma State
Started by GAMECOCK_FAN, Aug 12 2014 02:05 PM
Similar Topics
|Started By
|Last Post Info
Pinned 2015 NCAA Basketball Bracket Challenge
Started by GAMECOCK_FAN, 27 Feb 2015 In: SEC Sports → SEC Basketball Talk
- Hot 126 replies
- 3,283 views
hard money lenders oklahoma
Started by Waltermery, 25 Mar 2015 In: The West → LSU Sports → LSU News
- 0 replies
- 70 views
Tuesday Ticket Deal of the Week Baseball vs. Kennesaw State
Started by TheRealBrave, 24 Mar 2015 In: The East → Georgia Sports → Georgia News
- 0 replies
- 46 views
state farm loan calculator
Started by Waltermery, 23 Mar 2015 In: The West → LSU Sports → LSU News
- 0 replies
- 56 views
Baseball vs. No. 20 Miss. State -- Postgame Notes -- 3/22/15
Started by UKat, 22 Mar 2015 In: The East → Kentucky Sports → Kentucky News
- 0 replies
- 88 views
0 user(s) are reading this topic
0 members, 0 guests, 0 anonymous users


text:
- Gaming Guide
PS4 Remote Play is officially available for the Sony Xperia Z3 series
Sony's goal to bring the Playstation 4's Remote Play to its Xperia Z3 and Z2 family of phones and tablets is a step closer to fruition as Remote Play is now available for the Xperia Z3 series.
Playstation 4 firmware 2.0 is here, adds Share Play and a host of other features
Sony's latest firmware update brings the Sony Playstation 4 console to version 2.0, adding numerous new features including the much hyped Share Play. More details inside.
Roccat and Alienware announce exclusive partnership
Alienware and Roccat have announced an exclusive collaboration. More details on the partnership and what it all boils down to after the jump.
Want super fast storage? The OCZ RevoDrive 350 Series is finally here
OCZ's new RevoDrive 350 PCIe-based SSDs, which offers transfer speeds of up to 1.8GB/s, is finally here and is available in 240GB, 480GB and 960GB capacities. We have the pricing details here.
Rob Riggle to play Frank West in Dead Rising movie
Legendary Pictures will be releasing a Dead Rising movie, with Comedian Rob Riggle to play Frank West. More details about the movie after the jump.
More Sony devices to get PS4 Remote Play capabilities?
A Sony leak reveals new Remote Play compatible gadgets. Which products in the Xperia line will be getting it?
New Google Nexus Player announced
The market for set-top boxes is heating up! Google throws its hat in the ring with the new Google Nexus Player
ASUS announces three new ROG gaming notebooks - the ROG G751, G771 and G551
ASUS has just announced three new ROG gaming notebooks, which features Intel's recently refreshed Haswell processors as well as NVIDIA's new mobile GPUs based on the Maxwell architecture.
Samsung begins mass production of world's first 3-bit 3D V-NAND flash memory
Samsung has just announced that it has began mass production of the world's first 3-bit 3D V-NAND flash memory, with new drives sporting this memory expected to be out either later this year or earlier next year.
ECS announces mini-ITX Z97I-Drone motherboard
ECS has unveiled its mini-ITX Z97I-Drone motherboard to ride on the popularity of small form factor gaming rigs.
Aorus X7 Pro slim gaming notebook turbo boosted with dual GeForce GTX 970M GPUs
The sleekest SLI gaming notebook gets a massive performance boost with dual GeForce GTX 970M GPUs in SLI and makes surround gaming a possibility. Meet the Gigabyte Aorus X7 Pro.
Fujitsu launches new Extreme Series SSDs
The latest player to enter the local SSD market is Fujitsu, who has just announced its new Extreme Series SSD, which will be powered by an LSI SandForce controller.
Samsung is pulling out of the European notebook market
No one is unaffected by the declining PC market, not even giants Samsung. And according to reports, the company is said to be exiting the European notebook market.
Raidon's new InTank iR2770 internal RAID module lets you put two drives in RAID 0 or 1 easily
The Raidon InTank iR2770 is an internal RAID storage module that lets users easily configure two 2.5-inch form factor drives in either RAID 0 or 1. What's more it fits into a single 3.5-inch drive bay.
Explore the world of 'Destiny' with Google Street View
Bungie's new franchise, Destiny, doesn't come out till next week, but thanks to Google, you can tour the game world now with Google Street View.
SanDisk introduces new Ultra II SSD using TLC NAND
Flash memory giants SanDisk has just introduced the new Ultra II SSD, featuring new X3 NAND flash and nCache 2.0 technologies for better endurance and performance.
SingTel announces new Unlimited Fiber Plan, promises no traffic throttling
Web junkies rejoice, SingTel has just announced a brand new high-end fiber plan that promises uncapped downlink and uplink speeds and we have the screenshots to prove it.
Amazon buys Twitch for $970 million
Following earlier rumors that Google was prepared to pay US$1 billion for Twitch, Amazon has apparently swooped in and picked up the livestreaming video network for a mere US$970 million.
OCZ has a new entry-level SSD called the ARC 100
OCZ has just launched its new ARC 100 SSD, which is powered by its in-house developed Barefoot 3 controller and is targeted at entry-level and budget-conscious users.
The ASUS RT-AC87U AC-2400 class router will go on sale next week at Comex 2014
The ASUS RT-AC87U is the first Wave 2 802.11ac to hit our shores and it will be officially launched to the public at COMEX 2014 next week.


text:
User:Abrahamic Faiths
Hello and welcome to my profile. My username is Abrahamic Faiths, my real name will not be disclosed. I am 21 years old, and I am a resident of Scottsdale, Arizona, I have Asperger syndrome but that does not affect my editing and I will never try to use that as an excuse for anything. I will not go in too deep about my personal life, but I will discuss my Wikipedia life; I first learned of Wikipedia when I was 12 and I have always used it when ever I had a question. Now that I am older I have decided I want to contribute to this wonderful website. I am fascinated by different Languages, different Religions and different Cultures, I will be editing mostly in those areas. I am a very open-minded person, and I respect other peoples opinions, I believe all humans belong to one family and dispite our differences, we can all find a way to get along. I am getting better at editing Wikipedia as time moves on, but I am still learning, I really do try my best to be neutral with my edits, but being human I make mistakes every now and then, feel free to contact me if you have any questions or complaints about any of my edits, I will respond and do my best to correct the problem.


text:
After taking two weeks off for the holidays and a delay yesterday because of a missing juror, Testimony in the trial of a man accused of killing his former girlfriend inside of her Wichita Falls home nearly three years ago started back up this morning in a Denton County courtroom.
Russell Leath is sentenced to 12 years in prison. He'll get 172 days credit for time already served.


text:
Town, schools answer county complaint
By its own calculations, the Town of Tunica is now owed nearly a half million dollars in gaming tax revenue from Tunica County, money the county is withholding, it says, pending the resolution of a claim filed last October.
Funds that go to the Tunica County School District are also being withheld by the county, according to a counterclaim filed by TCSD against Tunica County.
Now, the claims and counterclaims may be one step closer to a resolution. Both the Town of Tunica and the Tunica County School District have asked the court to enjoin Tunica County to release the funds being held and cease withholding the gaming tax revenues in the future.
Gaming agent killed at Robinsonville office
In an apparent accidental shooting, John Ballard Gorman, 45, of Madison County was shot last Wednesday, January 21, and died on the scene at Mississippi Gaming Commission offices near Robinsonville.
Gorman, the father of two, had been with the agency since its inception and had just been promoted to Director of Investigations the day before the incident.
Meeting with the State Superintendent
State Superintendent of Education Dr. Carey Wright (left) and her staff meet with members of the Tunica County Committee for Educational Improvement last Friday, January 23, in Jackson.
NWCC offers test assistance for job seekers, adult students
Too few people in Tunica County are utilizing a valuable resource available to them now at no cost, and ABE/GED Transition Coordinator at Northwest Mississippi Community College Beth Little wants to get the word out.
Adult Basic Education classes are a path to higher education, a better job, and knowledge to help parents partner in their children's schooling.
"Our ABE program offers so much more than just a GED," Little told Tunica Rotary members last week, "And it's at no cost to the public."
More Articles...
Page 10 of 268
Latest News
Manhunt ends with arrest A manhunt that started...
Cookin with The Columns: Bring home the bacon! These bacon rollups are...
Debate introduces District 3 supervisor candidates to voters race first up The candidate debate...
New business opens downtown Delta Mini Mall owner...
Aldermen set new water, sewer rates; hearing planned The Board of Aldermen...
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8


text:
24 CFR 570.609 - Use of debarred, suspended or ineligible contractors or subrecipients.
The requirements set forth in 24 CFR part 5 apply to this program.
[61 FR 5209, Feb. 9, 1996]
Title 24 published on 2014-04-01
no entries appear in the Federal Register after this date.
Title 24 published on 2014-04-01
The following are ALL rules, proposed rules, and notices (chronologically) published in the Federal Register relating to 24 CFR 570 after this date.


text:
Instant "Members Only" Coupon Codes Emailed NOW When You Sign Up for FREE Membership!
You are already an existing user.
Do you want to opt back in?
Please check your email inbox for a confirmation message.
Just click the link to complete your subscription and receive your coupon codes.
Popular Destinations
- Mexico
- Caribbean
- Las Vegas
- Orlando
- Hawaii
- Florida
- New York City
- Costa Rica
- New Orleans
- San Francisco
The Parisienne Hotel
With a stay at The Parisienne Hotel in Blackpool (North Shore), you'll be minutes from Blackpool Central Library and close to Blackpool Pleasure Beach. This hotel is within close proximity of North Pier and North Pier Theatre. Read More About The Parisienne Hotel
The Parisienne Hotel Deals & Specials
- Free Breakfast
- Lounge/Bar
(+) = Additional fees may apply for the use of these amenities.
(*) = Free, some restrictions may apply.


text:
Honor roll at Tunica Middle includes 50 students
Tunica Middle School Principal Dr. Bacardi Harris announces the honor roll for the third nine weeks of the 2013/14 school year. Students with All A's are included on the principal's list. on the honor roll have A's and B's.
Latest News
Manhunt ends with arrest A manhunt that started...
Cookin with The Columns: Bring home the bacon! These bacon rollups are...
Debate introduces District 3 supervisor candidates to voters race first up The candidate debate...
New business opens downtown Delta Mini Mall owner...
Aldermen set new water, sewer rates; hearing planned The Board of Aldermen...
- 1
- 2
- 3
- 4
- 5
- 6
- 7
- 8


text:
Jobs Companies & Reviews Companies Salaries Interviews
Hiring? Post a Job
Linux Administrator Interview Questions
Sort: Popular Date
Sort: Popular Date
Did you mean companies matching "Linux Administrator"? See Companies
Mar 16, 2011
"What happens from when a server first has power applied until there is an on-screen login prompt?"
Mar 16, 2011
"What happens from when one types "simplyhired.com" into a web browser until the page is displayed?"
Oct 26, 2013
"Questions on the first step were in depth and reasonable for a Senior Linux admin. The entire linux LAMP stack is covered.
Nov 6, 2014
"I would not say it is one specific question but the technical interview is pretty in depth."
41–50 of 125 Interview Questions


text:
Find an Internship or Volunteer in Hewlett, New York ( NY )
If you're looking for internships or volunteer work in Hewlett, New York or even to get a summer job, you've come to the right place! With over 30,000 around the county, chances are that GrooveJob.com has the right job for you and wants to be your only source for finding internships and volunteer positions in Hewlett, New York.
We've got plenty of intern and volunteer work in Hewlett, New York. Check out the list of employers below who have positions in Hewlett. Remember to check back with Groovejob.com on a regular basis. We add new internships and volunteer jobs listings to our database every week, so you'll find new opportunities every time you check back with us.
|Browse by Employer (Click On Logos)
|Click for Intern or Volunteer at SEAWORLD ADVENTURE PARK
|Click for Intern or Volunteer at INROADS
Jobs by Title
PSEGLI Summer Intern - T&D
Req# 17445BR Title PSEGLI Summer Intern - T&D Location Hewlett - NY Job Function Transmission and Distribution Job Description...
Hewlett, NY
Sports Running Club - Summer Internship - Graphic Design Intern
Job# 453279736 Club has developed a robust internship program to provide college students (undergraduate and graduate levels) with...
New York, NY
MBA Treasury Intern
Impact As a Treasury Intern you will have the opportunity to work on projects across Treasury Operations, Global Treasury Planning, and...
Stamford, CT
Treasury Intern
Impact As a Treasury Intern you will have the opportunity to work on projects across Treasury Operations, Global Treasury Planning, and...
Stamford, CT
Risk Management Intern
Impact As an Assistant Risk Management Intern Analyst you will work with the other members of the Risk Management staff to finalize the...
Stamford, CT
Audit Intern
Impact As an internal audit intern , you'll work closely with team members to analyze various aspects of our company's operations to test...
Stamford, CT
MBA Finance Intern
Impact As an intern at Pitney Bowes you will work directly for and with leaders of our Global organization. You will help engage and...
Stamford, CT
MBA Finance Intern
Impact As an intern at Pitney Bowes you will work directly for and with leaders of our Global organization. You will help engage and...
Stamford, CT
Branch Manager Trainee Intern
General Responsibilities: Jump-start your career as an Intern with the #1 car rental company in the world! Hertz offers a fun, energetic,...
Lynbrook, NY
Production Intern - Banana Republic
GENERAL SUMMARY: The Intern for Production role is an entry level pre-manufacturing function, responsible for facilitating the approval...
New York, NY
Digital Product Management Associate Internship
…passion for technology. Were not your average IT shop, and this isnt your average internship . In your 10 weeks with GapTech (starting...
New York, NY
Retail Management Intern
Title: Retail Management Intern Function: Sales Department: Store Reports To: Department Manager Direct Reports: Sales Advisor(s) (dotted...
New York, NY
IT Intern
We are seeking a summer intern in our NYC office, this will be…will be a 6-8 week paid program. IT STUDENT INTERN Under the general...
New York, NY
CFS Accounting Internship Program
Attention all college students, CFS has a summer internship for all Accounting and Finance majors in the New York City area! Duties of...
New York, NY
KeyBank Real Estate Capital UG Intern - Summer 2015 - NYC
…rotational analysts, and advanced degree individuals as follows: - As a KeyBank intern , you will gain hands-on experience working on...
New York, NY
Marketing Internship
*Work with the marketing department with updating project information and other data entry *Assist in organizational and*Provide Graphic...
New York, NY
Print Production Intern , Summer
Job Posting Title: Print Production Intern , Summer 2015 Job Description: Print Production interns perform quality control for all printed...
New York, NY
Summer 2015 Veteran Internship Program
Ref#: 21623 Date published: CBS Business Unit: CBS Corporate Job Type: Paid Intern Job Schedule: Full-Time Job Location: New York, NY, US...
New York, NY
Summer 2015 Circulation Internship
…telling us: How you would increase subscriptions for the Wall Street Journal? Click here to watch our summer internship program in...
New York, NY
Youth Sports Organization - Summer Internship - Business Development & Strateg
Job# 453279735 The project will include opportunities for the intern to exercise autonomy in developing and implementing a plan for...
New York, NY
|<< First < Previous 1 2 Next > Last >>


text:
Proposed taro ban 'detrimental' –Central Bank of Samoa
APIA, Samoa --- Any ban placed on Samoan talo would be detrimental to our limited export base, the Central Bank of Samoa says.
This warning comes in the wake of a proposed ban being looked at by American Samoa against Samoan talo, in a bid to protect their local growers.
"Any ban to current commodities exported is detrimental to our limited export base," said CBS Chief Manager of the Monetary Stability Group, Benjamin Pereira, in an email.
"Based on taro export data that CBS collects, American Samoa has receipted on an annual average, from 2000 to 2012, just above SAT$900,000.00 (US$383,000).
Taro export are on 2000-2012 averages equivalent to 2.2 per cent of total annual exports, he said.
"American Samoa is currently our biggest market for taro export however, since the improvement in domestic supply of taro and the beginning of exporting again to New Zealand in 2010, the New Zealand market has significantly gained its market share.
He described this as "encouraging," adding, "Efforts to improve exports of other commodities are also encouraging."
Meanwhile Tautua Samoa leader, Palusalue Fa'apo II said the proposed ban could pave the way to more export bans.
Last week it was reported that American Samoa is working on a proposed ban of Samoan taro with their Attorney General.
This happened just two weeks after Samoa's Small Business Enterprise Centre and American Samoa's Chamber of Commerce signed a Memorandum of Understanding to develop each nation's economy.
According to the SBEC the Trade Fair was aimed at showcasing small businesses to the Samoan public as well as seeking trade opportunities to supply local retailers, hotels and restaurants and opportunities to export to American Samoa.
Palusalue said it saddened him to read this proposal in the newspaper, as the very reason for the Fair was to strengthen trade between the two Samoas.
"But now this news has come up it is really sad for our taro exporters," he said.
"Who knows their next move might be stopping the vegetables and so forth.
"It is fine for the Government to address the issue when they meet in their high level talks with the leaders of American Samoa, it is all they are doing.
"They said they want to work together but the way things are going it is not working together they are competing with each other."
He said with so much going on in the world of trade – with free trade talks between the Pacific, Australia and New Zealand and high level talks with the World Trade Organisation, the proposed taro ban would only add a burden to farmers.
"If you go to the market now, it is overflowing with taro we need exports and we need the exports to American Samoa to help our farmers here," he said.
"The taro is not enough in terms of exporting them.
"I believe through these talks the government should address the issue."
He said despite American Samoa wanting to protect their local growers, he believes the quality of their taro is not up to the same standard as Samoa.
« View more Latest News stories
...or view more articles related to these topics:
...or try these related articles:
Latest edition
Latest news
- Wed 25 Feb - Fiji: Private sector says EU fisheries report inaccurate
- Wed 25 Feb - Tonga: World Bank Regional Vice President to meet Tongan Prime Minister today
- Wed 25 Feb - Fiji: Fiji disciplinary forces support UN Convention Against Torture
- Tue 24 Feb - Tonga: Princess Tu'ipelehake fights for Fatai residence in Court
- Tue 24 Feb - Tuvalu: Tuvalu and Kiribati push for legal agreement on local crews for purse seine vessels in PNA waters
- Tue 24 Feb - Fiji: Fiji continues to expand foreign relations
- Tue 24 Feb - Tuvalu: Tuvaluans residing in Fiji need to extend their visas if they want to stay longer
- Tue 24 Feb - Tuvalu: Peak tide affects Tuvaluan communities living in coastal and low-lying areas
- Tue 24 Feb - France: Agriculture must change
- Tue 24 Feb - Australia: Pacific connected: A regional approach to development challenges facing island nations


text:
In music's worst draughts, there sometimes seems like no hope for "spicing things up." Instead, I find myself needing to throw things all the way back. These are songs that I feel like I've listened to over and over again, but still haven't gotten sick of. Hopefully you won't be sick of them either. 1. […]
Tag Archives | The Cure
WPGU @ Lollapalooza 2013: Preview
By Boswell Hutson on July 31, 2013 in Music, Music News Comments { 1 }
For the past 5 years, Lollapalooza has been a staple of my summer schedule. I will never forget my dad walking me down to the gates when I was 15, somehow allowing me to enter this lawless and crowded mass of humanity all by myself, before I even had my driver's license. I had grown […]
Monday Mixer
By Boswell Hutson on April 16, 2012 in Music, Music News Comments { 1 }
Listen: Clear Eyes – Nico Segal & Vic Mensa "Clear Eyes" – Nico Segal & Vic Mensa – The trumpet player and rapper from Chicago's Kids These Days put together an extremely chill song for kicking back when times get tough for the upcoming finals. Check out Segal's "Illasoul" mixtape here for more. Listen: Welcome Home, Son […]
February 16, 2015 - 8:02 am Black Sun Death Cab for Cutie
February 16, 2015 - 7:57 am Zero Smashing Pumpkins Mellon Collie and the Infinite Sadness
February 16, 2015 - 7:52 am Collegiate Peaks Grandkids
February 16, 2015 - 7:49 am I Wanna Get Better Bleachers
February 16, 2015 - 7:45 am Come Original 311 Soundsystem


text:
Forney Industries 1/8" Acid Core Solder Wire 40% Tin / 60% Lead 1/4lb 38103$6.99
General purpose solder for sweat fittings, sheet metal joints, gutter work and other big jobs. Works well on galvanized sheet, copper, steel, nickel plate, brass and tin plate. Not for electrical connections.Learn More
Silent Strike Aluminum Thumb Loop Folding Knife 60 01 2210092$6.99
Product Features:
- Aluminum Handle
- Stainless Steel
- Folding Design
- Lock Back
- Thumb Loop for Opening
- Belt/Pocket Clip
- Handle- 4-1/2 inches long by .25 inches thick
- Blade- 3-1/2 inches long
Pro Grade 1/2" Drive Increaser 3/4" Drive 14003$6.99
This lets 1/2" Drive Ratchets accept 3/4 Drive socketsLearn More
Hobart Clear Face Shield Replacement 770579$6.99
- Clear replacement lens
- Fits 770118
- For cutting and grinding
Goldblatt Tools 8 inch Stainless Steel Taping Knife G05638$6.99
The Goldblatt Pro-Grip series taping knives are simply the best drywall finishing knives on the market. Developed using a panel of professional finishers, these knives offer the flex and feel that will satisfy even the most demanding users. Perfectly balanced feel with ultra-flex blades to ensure perfect feathering and finishing. Crafted with a unique tapered ergo-grip and rugged hammer-end. Available in 6-Inch, 8-Inch,10-Inch,12-Inch, and 14-Inch sizes.
- Pro-Grip tapered handle reduces fatigue
- Convenient hammer-end
- Rust resistant stainless steel blade
- 1-Inch aluminum spine maintains blade straightness
Learn More
Silent Strike Pakkawood Handle Folding Knife 60 01 1210017$6.99
Product Features:
- Stainless Steel
- Pakkawood Inlay Handle
- Liner Lock
- Folding Design
- Thumb Stud for Opening
- Handle- 4 inches long by 1/2 inches thick
- Blade- 3 inches long
Stack-On, 13" Handy Storage Box (Red/Gray), GRM-13$6.99
Product Features
- Riveted nickel-plated catch
- 7-compartment removable tote tray including 4 "easy scoop" compartments for small parts.
- Fully lockable with padlock eye.
- Available in Red.
- Dimensions: 13" W (33cm), 6.875" D (17cm), 6.125" H (16cm)
Hobart Pencil Scribe 770072$6.99
Convenient pen-style scribe with tungsten carbide tip clips onto shirt pocket.Learn More
Hobart Straight Head Chipping Hammer 770066$6.99
Our all-steel, heavy-duty chipping hammers feature hardened heads for long life. The spring handle absorbs shock of contact while cleaning and chipping welding debris. Heads have a chisel on one side and a point on the other to get into tight spots. Straight head with shock-resistant spring-steel handle with hanging loop.Learn More
Hobart Large File-Type Tip Cleaner 770085$6.99
Stainless steel wire files for cleaning the gas orifices of cutting and brazing tips. Twelve files from 49 to 75, plus a tip file in aluminum case.Learn More