text:
Devices and systems related to surgical fasteners and more specifically to surgical fasteners suitable for use in both open procedures, and minimally or less invasive procedures where the operative site is remote from the surgeon.
1. A surgical fastener for deployment through a device, the fastener comprising:
a first anchor member;
a second anchor member;
a connecting portion separating the first and second anchor members; and
where at least the first anchor member and the second anchor member each are expandable from a first state to a second state where the second state is of a larger size than the first state.
2. The surgical fastener of
3. The surgical fastener of
4. The surgical fastener of
5. The surgical fastener of
6. The surgical fastener of
7. The surgical fastener of
8. The surgical fastener of
9. The surgical fastener of
10. The surgical fastener of
11. The surgical fastener of
12. The surgical fastener of
13. The surgical fastener of
14. The surgical fastener of
15. The surgical fastener of
16. The surgical fastener of
17. The surgical fastener of
18. The surgical fastener of
19. The surgical fastener of
20. The surgical fastener of
21. The surgical fastener of
22. The surgical fastener of
23. The surgical fastener of
24. The surgical fastener of
25. The surgical fastener of
26. The surgical fastener of
27. The surgical fastener of
28. A surgical fastener for deployment through a device, the fastener comprising:
a first means for anchoring the fastener;
a second means for anchoring the fastener and;
a connecting portion separating the first and second means for anchoring.
29. The surgical fastener of
30. The surgical fastener of
31. The surgical fastener of
32. The surgical fastener of
33. The surgical fastener of
34. The surgical fastener of
35. The surgical fastener of
The present invention relates to surgical fasteners and more specifically to surgical fasteners suitable for use in both open procedures, and minimally or less invasive procedures where the operative or surgical site is not directly accessible by the surgeon.
Surgical fasteners are known to be an alternative to traditional suturing techniques for procedures involving tissue closure, connection, or repair. One undesirable aspect of manual suturing is that the suturing process adds time to the overall surgical process. Moreover, manual suturing often requires that the operative area is readily accessible so that the medical practitioner can manipulate the suture and associated needle through both sides of the tissue, connection or repair site. Presently, surgical fasteners are known to provide a means to close an open surgical incision or wound, hold together pieces of soft tissue, attach devices to tissue, or repair torn tissue in orthopedic/musculoskeletal applications. Such surgical fasteners are often used where there is adequate access to the operative area, or for invasive, open procedures.
Due to the inherent risks and complexities of invasive surgical procedures, there is an increasing need for the ability to perform surgical procedures in a minimally invasive manner. In most cases, the recuperative time and lowered expense of a minimally invasive procedure makes it a far more desirable option to an alternative comparable invasive/open surgical procedure. The use of surgical fasteners in minimally invasive procedures may be desirable to increase the speed and efficiency of the procedure. Such fasteners may also open the possibility of performing a minimally invasive procedure for what was previously limited to an open surgical procedure.
In addition, suturing techniques requires considerable skill and dexterity especially when tying knots in the suture or otherwise manipulating the suture. The ability of a medical practitioner to manipulate a suture as well as knot the ends of the suture are further complicated when the site is not directly accessible to the practitioner. In such cases, even if the complexity of suturing does not prevent the procedure from being completed in a minimally invasive manner, the length of the procedure is likely to increase.
Conventional fasteners do not easily lend themselves for use in minimally invasive surgical procedure. As one example, the complexity of the known fastener-delivery devices requires devices with large profiles and limited flexibility further thereby limiting the potential for such devices to access remote locations. Conventional surgical fasteners, especially, �I-shaped� or �H-shaped� fasteners are unsuitable for remote procedures due to their complex deployment mechanisms and inability to navigate tortuous pathways using access devices commonly used for minimally invasive procedures (e.g., catheters, introducer devices, scope-type devices such as endoscopes, bronchoscopes, colonoscopies, etc.). Examples of such fasteners and devices are discussed in U.S. Pat. No. 4,006,747 to Kronenthal et al., U.S. Pat. No. 4,235,238 to Ogiu et al., U.S. Pat. No. 4,669,473 to Richards et al., U.S. Pat. No. 5,941,439 to Kammerer et al,. U.S. Pat. No. 6,039,753 to Meislin, and U.S. Patent Publications US2003/0097148 to Valimaa et al, U.S. 2003/0187465 to Bailly et al. Each of the foregoing patents and/or patent applications is hereby incorporated in their entirety by reference.
The present invention includes a surgical fastener for deployment through a device (such as tubular device, a needle, cannula, catheter, etc.), where the fastener comprises a first anchor member, a second anchor member, and a connecting portion separating the first and second anchor members, where at least the first anchor member and the second anchor member each are expandable from a first state to a second state where the second state is of a larger size than the first state, where the larger size may be achieved by an increase in displacement (e.g., volume, profile, configuration, etc.) of a portion of the fastener or the entire fastener. For example, as it assumes the second state, the anchor may change in shape or conform to a profile that is of a larger size than the profile of the first state. Alternatively, or in combination, the increase in volume may be achieved by relaxing a previous state of compression of the fastener portion. In the latter case, the fastener portion may comprise a resilient material that is compressible, and/or the fastener portion may be hollow, or have a cavity, such that the outer perimeter of the anchor portion may be folded into the cavity to assume the first state, or compress the cavity to conform to a smaller state.
The surgical fastener may also comprise a first means for anchoring the fastener, a second means for anchoring the fastener and a connecting portion separating the first and second means for anchoring. Where the means for anchoring may be any of the anchor portions described herein.
The invention also includes a surgical fastening system comprising, a tubular member having a proximal and distal end and a lumen extending therebetween, the tubular member being sufficiently flexible to navigate tortuous anatomical passages within a human body, a distal portion located at the distal end of the tubular member, the distal portion having a distal tip being configured to pierce tissue, the distal portion having a lumen extending between the tubular member lumen and an opening in the distal portion, at least one surgical fastener slidably located entirely inside the tubular member lumen, where the surgical fastener comprises a first anchor member, a second anchor member, and a connecting portion separating the first and second anchor members, and an advancing member slidably located within the tubular member lumen such that advancement causes a distal portion of the advancing member to advance the surgical fastener through the tubular member. The system of the present invention may be directed to the desired site using a catheter-guidewire configuration, shaped catheter, a steerable catheter, a scope-type of device (e.g., such as endoscopes, gastroscope, colonoscope, bronchoscope, or any type of scope used to access sites within the body.)
It should be noted that alternate variations of the present invention include fastening system of the present invention used with conventional fasteners and/or fasteners of as described herein.
The present invention is useful in many surgical procedures requiring fastening systems, including but not limited to, procedures for fastening or repairing tissue or attachment of implant materials to tissue. The present invention is suitable for, but not limited to, use in the heart, stomach, gastro-intestinal tract, etc. While the faster and fastening system may be used in open procedures, the devices and systems may also be used in minimally invasive procedures where the operative site is remotely accessed using minimally invasive techniques including catheterization and/or endoscopic or similar means.
The inventive devices are especially suited for advancement via a minimally invasive technique by providing features which improve the ability of the surgeon to deploy the fastener with accuracy and effectuate a proper seal with the fastener. The minimally invasive technique also may allow for visual inspection of the placement of the anchor.
This application incorporates by reference an application filed Mar. 11, 2004 entitled �SURGICAL FASTENING SYSTEM� having application number ______.
The following illustrations are provided as variations of the present invention. It should be understood that there are many combinations of the present invention and that figures illustrating all variations of the invention would be numerous. Therefore, the invention is intended to include combinations of aspects and features of the illustrated embodiments, or combinations of the specific embodiments themselves.
In use, the fastener 100 may be delivered to the operative site when the portions of the fastener 100 are in the first state. Upon deployment, selected portions of the fastener 100 assume the larger second state. This configuration allows for delivery of the fastener 100 through an opening in the medium where the opening is smaller than a diameter (or other similar dimension) of the fastener 100 portions after deployment. In some variations of the invention, construction of the fastener 100 allows only a portion (e.g., a single anchor, both anchors, the connecting portion, or a combination thereof) to expand into the second state. In other variations, the entire fastener 100 may be constructed to assume the second state upon deployment. It is contemplated, that the various portions of the fastener 100 may expand differently as required for the particular application (e.g., one or more portions expand at a different rate, a different size, etc.)
Expansion of the fastener 100 from the first to the second state may be accomplished a variety of ways. For example, the fastener 100 may be constructed of a shape or material that allows compression of the fastener portion, either by application of a compressive force or application of a vacuum, etc. Alternatively, or in combination, the fastener 100 may include a material that swells or expands given the addition of a fluid (e.g., natural body fluids or fluids introduced during the surgical procedure.)
Examples of these materials include biodegradeable and non-biodegradeable polymers, elastomers, shape-memory alloys, other alloys, etc.) For example, carbonate copolymer, polyether ester copolymer, albumin, gelatin, starch, cellulose, dextrans, polysaccharides, fibrinogen, poly (D,L lactide), poly (D,L-lactide-co-glycolide), poly (glycolide), poly (hydroxybutyrate), poly (alkylcarbonate) and poly (orthoesters), EVA copolymers, silicone rubber and poly (methylmethacrylate). Particularly preferred polymeric carriers include poly (ethylene-vinyl acetate), poly (D,L-lactic acid) oligomers and polymers, poly (L-lactic acid) oligomers and polymers, poly (glycolic acid), copolymers of lactic acid and glycolic acid, poly (caprolactone), poly (valerolactone), polyanhydrides, copolymers of poly (caprolactone) or poly (lactic acid) with polyethylene glycol, PET, PETE, and blends thereof.
In any case, the second state of the fastener comprises a larger profile or configuration as compared to the first state. As stated above, this permits securing of the anchoring portions 102 104 about the medium and/or securing of the connecting portion 106 within the medium. Another advantage of the invention is that the opening in the medium created during deployment of the fastener 100 may be smaller than would otherwise be possible if the fastener did not expand into the second state upon or after deployment.
It is also contemplated that the fastener may incorporate a variety of additives, coatings, adjuncts, etc. For example, the fastener (or only portions of the fastener) may include a lubricious coating to improve advancement of the fastener in the delivery system. The fastener may include non-proliferative drugs, thrombogenic additives, non-thrombogenic additives, non-inflammatory medicines, additives to induce fibrosis for wound closure, anti-platelet, anti-coagulent, growth factors, gene-transductors, cell matrix, glue, cement, protein, hydrophilic, hydrophobic, lipidphillic, lipidphobic, or combinations where appropriate.
The invention also contemplates that the anchor portions described herein may be configured/suited for attachment of external devices/implants/objects/etc. For example, one possible use of the inventive fastener is placing the fastener in the wall of an organ, then attaching an implant to the organ's wall by attaching the implant to the anchor portions of the fastener.
It is contemplated that various methods known in the field may be employed to advance/retract the advancing member. For example, the advancing member may simply push the fastener using a linear or rotary type drive system. Alternatively, the advancing member may be an auger type system that advances the fastener with the assistance of rotatable vanes within the tubular member. A pneumatic, hydraulic, or fluid filled actuation may also be used to advance/retract the advancing member. The advancing member 156 may be a guidewire or other similar type device that is able to deploy the fastener 100 at the operative site. Although not illustrated, the fastener 100 may be removably attached to the advancing member 156 to improve accuracy in deployment of the fastener 100. In some variations of the invention, the fastener 100 is configured relative to the lumen 154 so that friction retains the fastener 100 within the lumen 154 until deployment of the fastener 100. In such cases, the wall surface and/or diameter of the lumen 154 may be selected to increase the sliding resistance of the fastener 100. In any case, the system will be configured so that upon deployment of the first anchor the fastener 100 will release from the device rather than pulling out of the tissue.
The system 150 also includes a distal portion 158 located at the distal end of the system 150. The distal portion 158 has a distal tip 160 configured to pierce tissue and has an opening 162 through which the fastener 100 exits the device. In some variations of the invention, the distal tip 160 is configured to prevent �coring� of the tissue to minimize the size of any opening created during deployment of the fastener. Instead, the tip 160 configuration has a sharpened area and a taper proximal to the sharpened area so that the tip 160 makes a small puncture and then dilates the opening in the tissue.
Although the fastener of the present invention may be delivered through any tubular device such as a cannula, a catheter, polymeric tubing, etc., the fastener may be part of a fastening system that permits deployment of the fastener in remote parts of the body through a variety of minimally invasive procedures. In such cases, the system may include a steerable catheter, or the system may be guided to the site via a separate catheter, a separate steerable catheter, a endoscope-type device, pre-shaped catheter, etc.
As discussed herein, upon deployment of the fastener 100 from the system 150, the fastener 100 portions shall increase in size from the first state to a second state where the larger size may be achieved by an increase in volume and/or profile a portion of the fastener or the entire fastener. The invention contemplates that the fastening delivery system 150 may be used to constrain the fastener 100 into the first state, via a compression mechanism. Alternatively, or in combination, the fastener 100 or portions of the fastener may be configured to increase in size given the application of a fluid. The fluid may comprise naturally occurring bodily fluid or fluid delivered by the fastening system or even fluid delivered via a separate device.
As discussed above, one of the functions of the inventive tissue fastener is to retain two pieces of tissue together, retain an implant to the tissue, or close an opening in tissue. The feature of the inventive fastener 100 relating to expansion of the anchor portions 102 104 permits placement of the fastener 100 using an opening in the tissue that is smaller would otherwise be required. Moreover, fasteners of the present invention may also be configured such that the central portion 106 expands into a second state as well. In such variations, expansion of the center portion may allow for expedited healing of the opening in tissue, or for closure and sealing of the opening in the tissue. In additional variations of the invention, the central portion 106 may be configured from a material that allows stretching of the center portion 106 during deployment. As shown in
In the illustrated variation, the opening 162 is located in a side wall of the distal portion 158. However, as discussed herein, the opening may be at the distal tip 160. It is contemplated that the tubular member 152 will have sufficient column strength to allow for penetration of tissue via advancement of the system 150. Accordingly, the tubular member 152 may be constructed of a material that provides sufficient flexibility and column strength. Alternatively, the tubular member 152 may include a reinforcing member 166, such as a coil, braid, or fiber reinforcement. Furthermore, as discussed above, the system 150 includes an advancing member 156 that permits advancement and/or deployment of the fastener 100. To improve advancement and deployment of the fastener, the tubular member and/or advancing member may be selected from materials that minimize the friction between the two members. Alternatively, or in combination, these items may include a lubricious coating to minimize friction. Although not illustrated, the system 150 may include an additional fluid delivery means, such as a fluid source where delivery of the fluid occurs via the lumen of the device, an additional fluid lumen, a separate catheter-type device for delivery of the fluid, etc.
Referenced by
Original Image


text:
Dewi Bridges
From Wikipedia, the free encyclopedia
Dewi Morris Bridges was the Bishop of Swansea and Brecon from 1988[1] until 1998.
Bridges was born in Beaufort, Blaenau Gwent on 18 November 1933.[2] He was educated at the St David's College, Lampeter and Corpus Christi College, Cambridge, and was ordained after a period of study at Westcott House, Cambridge in 1957.[3]
He held curacies at Rhymney and Chepstow (1960–63) after which he was Vicar of St James', Tredegar. From 1965 to 1969 he was a Lecturer at Summerfield College of Education, Kidderminster, and then Vicar of Kempsey. Later he was Rural Dean of Narberth and then Archdeacon of St David's, before his elevation to the Episcopate in 1988.[4]
- ^ City & County of Swansea
- ^ Who's Who 2008: London, A & C Black, 2008 ISBN 978-0-7136-8555-8
- ^ Crockford's Clerical Directory1975-76 Lambeth, Church House, 1975 ISBN 0-19-200008-X
- ^ Diocese of Swansea
|Church in Wales titles
|Preceded by
Benjamin Noel Young Vaughan
|Bishop of Swansea and Brecon
|Succeeded by
Anthony Edward Pierce
|This article about a United Kingdom bishop is a stub. You can help Wikipedia by expanding it.

subset: commoncrawl


text:
Business Assistance
Lakewood and Pierce County offer many opportunities for entrepreneurs or small businesses interested in starting or expanding operations. Economic Development can provide you with basic demographic and economic information about Lakewood and Pierce County. We also work closely with the Economic Development Board for Tacoma-Pierce County on business retention, expansion, and recruitment activities.
The Lakewood Chamber of Commerce provides 500 community businesses numerous resources including promotion, lobbying at the state and local levels and networking opportunities. They maintain a Community Events Calendar and can be reached at (253) 582-9400.
- WA State Department of Commerce
- Pierce County Financing Program
- U.S. Small Business Administration (SBA) offers programs that specifically support the needs of small business, such as loan assistance, equity financing, surety bonding, and financing to assist with acquisition, operation or expansion.
- South Sound Washington Business Center (SSWBC) has been working to take businesses from start-up to success to significance. Let them work with you to grow an idea into a business or take it to the next level. SSWBC is part of Community Capital Development, a non-profit community development organization serving small business owners and provides the following services:
- Washington Small Business Development Centers (SBDC) promotes economic vitality by providing advice, training and research to entrepreneurs and existing businesses statewide.
International Advantages and Regional Partners
- Strategic location midway between Asia and Europe.
- Puget Sound provides all-weather ports for ocean-going ships at the cities of Seattle, Tacoma, and Olympia.
- More than $40 billion worth of goods travel through the Port of Tacoma and Port of Seattle every year. Closer to Asia than any other North American seaports, the ports of the Pacific Northwest are premier gateways for products to and from North America.
There are many organizations that promote and support international trade and improved international relations. Some of the key ones are listed below. Extensive additional listings may be found at the Trade Development Alliance of Greater Seattle website.
- Export.gov
- Port of Tacoma Washington Council on International Trade (WCIT)
- United States Trade and Development Agency
- Washington State China Relations Council
- World Affairs Council (WAC)


text:
'If it is not good for us, it is not good for the Jews in the Negev'
Haredi parties intent on entering coalition by start of Passover
Ya'alon: You don't have to be in intelligence to know that Iran is lying
Negotiating parties split on Likud push to increase number of ministers
First-ever effort between hospital and taxi company to ensure newborns are taken home safely
Jerusalem Municipality shuts down 'filthy, rodent-infested' butcher shop in Mahane Yehuda market
*New Worlds: The brain's 3D compass*
*IQ is not enough – develop your EQ, too*
Eco Wave Power to establish its first Chinese power station
Watch: Technion students recreate Passover story with robots
Noble Energy walks away from Ruth C exploration license
Cyber Chief: 8% of ministry budgets should go to cyber security
Play it once again, Sam
Anti-Semitic fans pressure Hungarian rock band to cancel Israel concert
Concert review: IPO
Eytan's Fox's 'Cupcakes' opening in New York
Local hoops rocked by sudden death of Hap TA manager Shelef
Bruised yellow-and-blue looks ahead after Real blow
Israeli figure skater Bychenko places in world championships
Soccer: Israel trounced by Wales in key qualifier
Court convicts Olmert in Talansky retrial
Israeli rescue team to assist at Germanwings crash site
Legislators, environmentalists gather for launch of Knesset solar rooftop field
Top Hamas engineer, tactician convicted in Beersheba court
Exclusive: In exchange for freed tax funds, PA won't pursue Israel over settlements at ICC
Egyptian court convicts man and woman of spying for Israel
Abbas: Israeli-Palestinian relationship must change
US 'disappointed UN rights council continues to single out Israel'
Jewish Agency, IFCJ spar over Ukrainian immigration
Why are these South Africans wearing kippot?
Will or won't 'Borat' play Freddie Mercury in Queen biopic?
Limmud FSU marks Auschwitz liberation with emotional exhibition
Toulouse rabbi told to remove kippah at voting station
Analysis: Israel rooting for the Saudis
US says talks with Iran will go down to the wire
Egypt lists as terrorists top Muslim Brotherhood leader, 17 others from group
Officials: Iran indicating willingness to accept fewer centrifuges, ship out enriched uranium
Watch: Armed Houthi women threaten war against US, Saudi Arabia, Israel
Reverse course
Fundamentally Freund: Happy Land of Israel Day!
Detoxifying US-Israel relations
Letters to the editor
Right or wrong: Anti-Semitism or emasculation?
The blood libel Haggada
The turning point
Travel tech start-ups
On wings of carbon
Veterans: On target
Fusing modern and traditional in the kitchen
Book Review: Perceptive Haggada
Hot off the Arab press
Let's cooperate
Shabbat screenings
Passover perspective
On the field and in court
Happily Eva After: Times, they are a-interestin'
Music and the mamaloshen
What's the catch?
Kayaking for gold on the Kinneret
Why Tunisia?
Palestine – words matter but their meaning matters more
A nuclear deal with Iran – would it stick?
When words matter and when they don't
'מְלָגוֹת 'צוּק אֵיתָן
עוֹלִים חֲדָשִים
כּוּלָם רוֹצִים לִחְיוֹת
יִשְׂרָאֵל בְּמַבָּט פָּנוֹרָמִי
Getting a young start
A culinary festival starring the women next door
Global girl power
Apartheid South Africa: Israel edition
Sa'ar says national unity government is 'still on the table'
Israeli parties prepare legislative demands for Netanyahu's new coalition
With full term possible, Netanyahu may outlast Ben-Gurion (and Obama)
Arab sector turnout for recent elections reached 63.5%, polling data shows
Joubran: Polls have to include parties that don't pass threshold
Pope Francis pays tribute to Christians persecuted by ISIS during Palm Sunday mass
PHOTOS: Exercising the 'Right to Movement' at the Palestine Marathon
It's hard to be a Christian in Israel, but not because of prejudice
Unconvincing ISIS video shows Assyrian captive converting to Islam
'Those who hate Jews hate Christians': A closer look at US Presidential hopeful Ted Cruz
Green Leadership Conference in Hamalachim-Shahariya Forest
Scenic Lookout in Memory of Lt-Gen. Amnon Lipkin Shahak
Following in the Footsteps of Meir Har-Zion
Celebrating the 100th Birthday Anniversary of Sculptor Ahiam Shoshany
French Students Get to Know the Real Israel
Serbian PM Plants a Tree in the Grove of Nations
Sharing and Learning - German Forestry Students Intern with KKL-JNF
Israel and Finland Connect through the Environment
New Bio-filters Purify Surface Runoff Water for Reuse
Future Tour Guides Discover the Negev with KKL-JNF
The Shamir Drilling Project
Graduation Ceremony for Rosh HaAyin's Volunteer Firefighters
From Sunny Israel to Cold Canada
Walking for Water with KKL France in the Mountains of Eilat
JNF Ontario Community Mission Tours the Western Negev
Celebrating 50 Years of German-Israeli Diplomatic Relations
The Negev Conference on Agricultural Research and Development
Arava Open Day: Food Security for Israel and the Entire World
Living Green in Israel: the Contest and the Winners
Addressing Climate Change Conference
From Think Tank to Environmental Consultant
Award Winning Designer Launches Career as Wedding Dressmaker
Outdoor Fitness Israel: An Innovative Fitness Program Developed By UK Olim
The Burstein Brothers: 3 Oleh Siblings in the IDF
5 Tips to Land a Job in Israel
Starting Your Own Business in Israel: Real Life Examples
5 Strategies for Finding Your Professional Place in Israel
PetBNB:An Olah's Unusual Canine Startup
36 Hours in Southern Israel
Food Shopping In Israel
How Women Can Have it All in Israel
The Eisenberg's Long Journey Home
Why Retirees are Thriving in Israel
Thanks to Aliyah video
6 Frequently Asked Questions about Israeli Healthcare
Starting Over in Israel
VIDEO: Are you ready for the South?
Sovev Tel Aviv, Israel's Largest Biking Event
Running Through Color
Formula 1 Racecars Zip Through Jerusalem
Make yourself at home… in Israel
NBN Announces Bonei Zion Prize Recipients for 2015
NefeshB'Nefesh Provides Services to Bnot Sherut
How Doctors Are Impacting Israel's South
Over 1,000 IDF 'lone soldiers' attend personal errand day in Tel Aviv
03.30.2015 | 10 Nisan, 5775
Cooking Class: Lump it, love it
In disguise
In the Grain: Complementary and necessary
Independent fishing
Holiday In: Skewers from around the world
In Season: On the side
A truly festive 'Shulhan Orech'
Matza do about nothing
Cooking Class: Shaily's Balkan beat
New Traditions: An Innovative Passover Seder
Green eats
Cooking Class: Cooking with beer
06/27/2012 13:42
(photo credit:Courtesy)
Grilling fish can bring terror to the most seasoned cook. The thought of delicate fillets being scraped off of grill grates in sad little shreds or of watching gorgeous fish falling between the grates, only to be consumed by the fire instead of your family, is enough to make grill-veterans preheat their ovens. I have three stress-free and fool proof methods of getting a gentle smoke and char on your favorite fish.The first method is to coat and re-coat the grill grates with oil. This method builds up a non-stick coating on your grill allowing you to get a delicious char on your fish.The second method uses aromatic lemons, green onions and herbs to provide a bed for the fish. The fish will develop tons of flavor from the smoky grill and the fragrant lemons and herbs.Sturdy and thick cut tuna can take direct heat from the grill. Unlike a flaky fish, tuna is not likely to fall apart on the grill. All of the grilling methods can be used for a stove top grill as well as an outdoor grill.Grilled Salmon with Thai Basil SauceFor the sauce:½ cup fresh cilantro leaves½ cup fresh mint leaves½ cup Thai basil leaves or any fresh basil¼ cup fresh squeezed lime juice1 tablespoon grated fresh ginger2 medium garlic cloves1 small spicy chili pepper, optional¼ cup coconut milkSalt and pepper1. Add all of the ingredients to a blender or food processor and process until a sauce consistency.2. Gently warm the sauce in a pan over medium heat. Adjust seasoning with salt and pepper.For the fish:4 six-ounce skinless salmon fillets¼ cup oil2 limes cut in halfSalt and pepper1. Preheat the grill to medium. Be sure the grill is clean and free from any debris.2. Dry the salmon fillets with towels. The fish must be completely dry or it will stick.3. Rub the grill with an oil soaked towel. Close the lid and allow the oil to get really hot. 4. Oil the grill again and close the lid. This process coats the grill grates with oil and keeps the fish from sticking. 5. Finally, brush the fish with oil and season with salt and pepper and place on the grill "presentation side" down (the non-skin side is the "prettier" side and will face up on the plate). Brush the lime halves with oil and place on the grill. Grill the fish fillets for five minutes on one side. Do not attempt to turn the fish until the fish has caramelized and formed a crust. You only need to turn the fish once. The more you "play" with it, the more it will likely stick and fall apart.6. Turn the fish over, brush the grilled side with some of the sauce and continue grilling for another minute for medium fish.7. Serve the fish with additional sauce and grilled limes.Grilled Halibut Mustard-Caper GlazeHalibut is a lean fish and I prefer to grill it on top of aromatic citrus and herbs. While you can put it directly on to an oiled grill, halibut can quickly dry out and over cook. The bed of citrus and herbs protect the delicate fish and keep it moist. The mild fish pairs perfectly with the mustard-caper glaze and picks up flavor from the citrus and herbs. For the sauce:2 tablespoons butter or olive oil for parve preparation2 medium shallots, minced1/3 cup dry white wine¼ cup Dijon style mustard2 tablespoons fresh squeezed lemon juice1 tablespoon sugar2 tablespoons capers2 tablespoons chives, cut into ½ inch segmentsSalt and pepper1. Place a small sauce pan, lightly coated with olive oil, over medium heat. Add the shallots and cook until the shallots are translucent and soft (about five minutes).2. Add the remaining ingredients, except for the chives, and cook until the mixture has reduced to a thick glaze.3. Remove from the heat and stir in the chives. For the fish:2 lemons cut into thin slices4 scallions, cut in half lengthwiseSeveral sprigs of fresh thyme, tarragon and parsleyOlive oil4 six-ounce halibut fillets, skin-offSalt and pepper1. Preheat grill to medium. 2. Brush the grill with olive oil. Arrange the lemons, scallions and herbs on the grill to form a bed for the fish.3. Pat the fish dry and season with salt and pepper. Lightly brush the fish with olive oil.4. Place the fish, "presentation side" down (the non-skin side is the "prettier" side and will face up on the plate) onto the bed of lemons and herbs. Grill the fish for five minutes. Brush the fish with the glaze.5. Turn the fish over and brush with the glaze onto the "presentation side". Grill the fish for another five minutes until cooked through. Brush one more time on the presentation side to build up the glaze.6. Serve the fish with grilled lemons and favorite vegetables.Grilled Tuna with Tomato-Balsamic RelishI ask my fish monger to cut my tuna into 1 ½ inch thick fillets, or better yet, whenever possible, I buy a big chunk of the gorgeous fish and cut the fillets myself. I like my tuna rare inside with a deep crusty char on the outside. Tuna's big flavor can stand up to a sweet and sour tomato-balsamic relish. I like to serve this hearty fish dish with grilled eggplant and zucchini.For the sauce:extra virgin olive oil for sautéing and for the relish1 small red onion, sliced thinly6 garlic cloves, minced4 large tomatoes, seeded and diced (sometimes I use heirloom or yellow tomatoes)¼ cup balsamic vinegar2 tablespoons sugar½ cup basil leaves, torn Sat and pepper1. Place a medium sauce pan, lightly coated with olive oil, over medium high heat. Add the onion and sauté until the onion is caramelized and dark brown (about seven minutes).2. Decrease the heat and add the garlic and tomatoes. Cook, stirring occasionally until the tomatoes let out their juices and begin to reduce (about 15 minutes). 3. Add the balsamic and sugar and stir to combine. Continue cooking for another five minutes. Remove from heat and fold in the basil leaves. Adjust seasoning with salt and pepper.4. Drizzle two tablespoons of extra virgin olive oil into the relish.For the fish:4 six-ounce tuna fillets, cut 1 ½ inch thickOlive oilSalt and pepper1. Preheat the grill to medium high. 2. Pat the fish dry and salt and pepper to taste. Lightly brush the fish with olive oil.3. Place the fish onto the grill and grill for two minutes. Turn the fish and continue cooking for another two minutes for rare tuna.4. Place the fillets on a platter and mound the relish on top of each fillet.Chef Laura Frankel is Executive Chef for Spertus Kosher Catering and author of Jewish Cooking for All Seasons, and Jewish Slow Cooker Recipes.
Follow @JPost_Lifestyle
Your comment must be approved by a moderator before being published on JPost.com.
Disqus users can post comments automatically.
Comments must adhere to our Talkback policy. If you believe that a comment has breached the Talkback policy, please press the flag icon to bring it to the attention of our moderation team.
Sign up for our newsletter
Please insert a valid email address
By signing up to The Jpost mailing list, I hereby


text:
Simply Divine Bed and Breakfast
No availability found through our partners. Please contact the business directly or check some of our recommended alternatives.
- Continental Breakfast
- Swimming pool
- Restaurant
- Free High-Speed Internet
- Free parking
- Pets allowed
Photos of Simply Divine Bed and Breakfast
Travelers also recommend:
TripAdvisor Reviews Simply Divine Bed and Breakfast Dunn
Travel Blogs from Dunn
Really a Road Trip Now!
... course led us to assigning each other Scooby Doo characters. Wasn't difficult, I bet you can all guess who got who lol. Since Hayley was out of action I drove us out of Washington, D.C. in spite of the sat nav being a bit ****, and onto the i95 where we stayed all day today and will continue on tomorrow! A single stop at Denny's for lunch and one stop for gas and in total I covered 316 miles today until ...
Clemmons Forest Hike
... area near Beddingfield Creek where a large number of ferns were growing in the floodplain of the creek. The Demonstration Trail makes a double loop and at the far end, we got on the Watershed Extension Loop that runs along the northern perimeter of the forest. There was some nice fall color along this area as we hiked along creeks and through powerline clearings. Much of the best fall color to be seen was along these clearings ...
Stopping in Raleigh, NC to visit Aunt Irma
... empty lot, then drive to Jeep to Aunt Irms. Aunt Irma looks good, says she feels good but hates all the tubes! We talked about Grandkids, great-grandkids, football and Enno and Gui. They are in Vero Beach and Gui isn't feeling too well. Her bad is very painful but she was determined to come to the states because her Grandkids were coming to visit. I know Aunt Irma would love to see them again. Her assisted living facility is lovely - even if she is ...
Road Trip!
... asphalt to finally arrive safely to a home cooked meal at Mom and Dad Hall's house in North Carolina.
A few highlights:
- Our highest ever night camping at 12,000ft at the base of four majestic 14ers in Alma, CO.
- The trip up highway 82 from Aspen to Leadville ...
Manassas to Fayetteville via Bull Run
... main battle lines staying between the guns, keeping to the main parts of Henry House. But at 96 degrees Fahrenheit, we did not stay out in the open for long.
We eventually set off for the drive to Fayetteville and soon needed Petrol, sorry Gas. At the same time found James a Taco Bell so he could eat. The rest of us opted for a Burger King later on after refuelling the car with $60 dollars for a full tank (�37). This ...


text:
EDWARD SCARDIGLI had a passion for helping people, and he got plenty of chances as a Philadelphia firefighter.
For most of his 26-year career, he was assigned to Engine 16 in West Philadelphia, one of the city's busiest companies.
He managed to emerge unscathed from two of Philadelphia's most horrendous fires more than three decades ago, the Gulf Refinery in 1975, in which eight firefighters were killed, and the ARCO refinery fire in which then-Mayor Frank Rizzo was injured.


text:
3-4 Down Lineman
View Single Post
All Pro
Join Date: Nov 2004
Age: 26
Posts: 934
Rep Power:
3-4 Down Lineman
Since plenty have been wondering who would fit in our Defense as a 3-4 DE I decided to do a little research.
One of the greats, Richard Seymour of the Patriots is 6-6 and wieghs in @ 305. Anthony Weaver of Baltimore is 6-3 and 296 and Kimo Von Oelhoffen of Pittsburgh is 6-3 @ 297. Those are all good 3-4 DE's in successful defenses.
Our current Defensive Ends in our system are Robaire (Who I acutally like) who list at 6-4, 310 lbs. and Gary Walker 6-2, 305 lbs. Neither one of them has noticeable speed and i think that may be part of why we can't get a good pass rush even though it is mostly the linebackers job in the 3-4 Defense to get to the QB.
Marcus Spears is 6-4 @ 298 and as previously said has room to add weight if needed. Shaun Cody is also 6-4 and 295 lbs. I would like to see us get either of these guys (Spears particulary, because of his speed). I also like the way Spears played in the Senior Bowl today. From what I saw he had alot of patience and good awareness. He also stuck with a play and never gave up.
One more thing, Mel Kiper said today that scouts have taken more notice of him and he could be moving down to about the Top 15 in the draft.
"Get ya hands off me!!!"-DROB
View Public Profile
Find More Posts by AndreJ


text:
VIEWS: 24 PAGES: 2 CATEGORY: Childrens Literature POSTED ON: 12/5/2009
Author Shanti A
Author: Shanti A. Parikh (Assistant Professor of Anthropology, Washington University, USA) Email Address: firstname.lastname@example.com Title: Private Acts, Public Shame: Reading Youth Love Letters as National Romance in Uganda The urgency of HIV/AIDS has necessitated that once private matters of sexuality become part of public discourse. The AIDS epidemic is just one in a series of sexual realities that in recent years has transitioned the sexual from private to public. This shift, largely motivated by the public health sectors and the accompanying rationalizations of medico- moral rhetorics, is accessed and reworked in the daily lives of individuals. This talk examines youth love letters from rural Eastern Uganda as representative of ways that sexual relations transition from private interactions to public scrutiny. Love letters have become standard courtship convention among sexually curious youth in Uganda. Highly stylized and carefully crafted letters clandestinely pass through go-betweens before finding the anxiously awaiting recipient. This is illustrative of how secrecy and sneaking as central realities in youth relationships. While amatory epistles are written to convey intimate emotions between two young lovers and firmly request confidentiality, they implicitly demonstrate an awareness of a larger audience. This audience typically includes from peers who judge their romantic prowess based on popular culture notions of modernity to adults who seek to intervene and delimit youth access to sexual liaisons. The proliferation of sex talk in Uganda generated by public health campaigns and popular culture provides youth in Uganda with condensed codes for negotiating sexual relationships and for managing the relationship's public face. My central question regarding public discourses is not how the circulation of sex talk is used to enforce morality or create new ideas about sexual relationships. Rather, I am interested in how love letters are homemade responses to local, national, and global flows of sexual imagery, romance, and shame that circulate with increasing ease around Uganda. An analysis of over 250 love letters and 2 ½ years of ethnographic research reveals that young males and females traverse the pubic in very different ways. Boys are highly performative in their letters and use emotional display to establish relationships and initiate sex. Girls, conversely, use letters to maintain and negotiate the terms of the relationship, often by expressing disappointment over the actions of their love interest. To understand these differences is to gain access to the way class, gender and age converge around the access to and management of public reputation. Implicit in any discussion of the way youth mediate intimacy and their public reputation is the notion of shame, which motivates the need for secrecy. Because of HIV/AIDS and other reproductive concerns there are heightened stakes for secrecy around youth sexual exploration and a widened gap between authoritative adults and sexually maturing young people. The entire process of love letters—from the conditions under which they are written, to the way in which they are circulated to their response from their intended recipient and the community at large— provides an ideal window into of the dialectic between regulation and romance of youth sexuality.
Pages to are hidden for
"Author Shanti A"Please download to view full document


text:
I updated my previously rejected item and thinking about resubmitting. Any suggestions?http://akingn.sidmad.com/cloussi/index.html
Helpful Information
- Please read our community guidelines. Self promotion and discussion of piracy is not allowed.
- Open a support ticket if you would like specific help with your account, deposits or purchases.
- Item Support by authors is optional and may vary. Please see the Support tab on each item page.
Most of all, enjoy your time here. Thank you for being a valued Envato community member.


text:
- Home
- News
- People
- Events
- Jobs
- Resources
- Store
- Subscribe NowLimited Time Offer
Memorial Hospital buys 100 acres in Shiloh
- Lisa R. Brown
Less than six months after BJC HealthCare closed on its purchase of 112 acres in Shiloh, Ill., Belleville's Memorial Hospital snapped up 100 acres next door.
Memorial Hospital is buying 100 acres at Frank Scott Parkway and Cross Street for an undisclosed amount. With BJC's recent nearby land purchase at about $192,000 an acre, the price tag could reach as high as $20 million for Memorial.
Memorial Hospital is keeping quiet about its expansion plans. "As this area's health-care leader, we are aware of the need to expand services in the communities we serve -- acquiring this property allows us to analyze the community need and plan accordingly," Memorial Hospital spokeswoman Anne Thomure said.
Memorial's purchase includes the O'Fallon Sportsman's Club at 1450 Cross St., which is located on 22 acres. The club's parcel comprises the largest piece in the redevelopment area. The club will relocate to property on Scott-Troy Road near Hagemann Road next year.
The remaining 78 acres are being sold by six property owners. Four of the six sales have closed, and two more sales, totaling 7 acres, are expected to close in June.
Terry Johnson and Dan Johnson of Johnson Properties Inc. represented both Memorial Hospital and the sellers in the transactions. "The Sportsman's Club has title to their new property," Terry Johnson said. Citing a confidentiality clause, he declined to comment about the hospital's plans for the site. "We're excited about the sale. We've spent a year working on it."
Memorial Hospital, located at 4500 Memorial Drive in Belleville, is owned by Protestant Memorial Medical Center Inc., a not-for profit corporation based in Belleville. The community hospital has 2,200 employees and more than 300 physicians. Memorial, which also has a 108-bed skilled nursing facility on its campus, has the busiest emergency room in the Metro East with 50,000 annual visits. The hospital had $220 million in revenue in 2007.
Memorial Hospital has been in growth mode since Mark Turner, former chief operating officer at Memorial, was named president in August 2006. The hospital is currently in the midst of a $4.5 million expansion and renovation of its ER to increase its capacity to 60,000 patients annually.
Turner told the Business Journal in April 2008 that the hospital is closely monitoring growth and population patterns. "In our market, several factors are contributing to the growth -- certain parts of our market are growing and the convenience of the hospital location has a lot to do with it," he said in the April interview. "And as access to care changes, more and more patients will come to the hospital if they are unable to find access to care with physicians in the community."
Paul Wunderlich, former chief executive of St. Luke's Hospital in Chesterfield who now has a health-care consulting firm, Health Care Advisory Panel LLC, said a sizable land purchase by a hospital indicates the hospital will ultimately build on the site. "If you're a hospital, the only reason you buy land is to invest in patient facilities," he said. "It could be anything from acute care facilities, to ambulatory care services or medical office space."
Land immediately west of Memorial Hospital's newly purchased property was acquired by an affiliate of BJC HealthCare earlier this year. The BJC affiliate, Progress East, bought a 112-acre parcel of undeveloped ground in Shiloh in January for $21.6 million. BJC's property fronts I-64 and is located between the interstate and Frank Scott Parkway, immediately east of Dierbergs Green Mount Crossing.
BJC has not announced its plans for the property but spokeswoman June Fowler said the population growth in the area prompted the decision to buy land in Shiloh. The St. Louis-based BJC HealthCare system has 13 hospitals, including Alton Memorial Hospital in Alton. BJC had net revenue of $2.9 billion last year. "If you look at trends in O'Fallon and Shiloh, with building permits and approved developments, it's obvious it's a real growth corridor," Fowler said. "The highway access is excellent and the anticipated growth at Scott Air Force Base, this is an area that's going to see a need for additional health-care services in the future."
Shiloh is among the fastest-growing communities in the Metro East. According to U.S. Census Bureau estimates released in June 2006, Shiloh's population grew 38.4 percent from 2000 to 2006, from 7,643 residents in 2000 to 10,575 residents in mid-2006.
Memorial Hospital's 100-acre property fronting I-64 is located near the planned extension of Frank Scott Parkway, which will link the roadway east of Cross Street to Scott Air Force Base. The airbase employs more than 14,000 active duty military, Air National Guard members, military reservists and civilians.
"The population to the east is growing," Thomure said. "Acquiring property in that area only serves to help Memorial meet community need and demand for Memorial's high-quality services well into the future."
Development epicenter
Memorial Hospital's land grab puts it in the center of the booming I-64 corridor with several large-scale commercial developments recently completed or in the pipeline.
O'Fallon, Mo.-based McEagle Properties' $400 million Three Springs at Shiloh project at Green Mount Road and Frank Scott Parkway is located next to both BJC and Memorial Hospital's parcels.
Three Springs at Shiloh, planned for several phases on 194 acres, will have a high density senior living component on 10 acres within the development, in addition to 468,651 square feet of retail space; 38,400 square feet of restaurant space; and 432,175 square feet of office space.
If you are commenting using a Facebook account, your profile information may be displayed with your comment depending on your privacy settings. By leaving the 'Post to Facebook' box selected, your comment will be published to your Facebook profile in addition to the space below.
Most Popular
- Most popular
- Slideshows
- Emailed
- Mobile
- Jimmy Sansone launches clothing line aimed at Midwesterners
- Jury sides with children in fight for Robert Kaplan's millions
- What the Arcade Building apartments will look like
- World Health Organization: Monsanto's weed killer "probably" causes
- Graybar acquires Georgia-based automation company
- Where Missouri ranks as a place to retire
- St. Anthony's to end affiliation with Cardinal Glennon
- Wash U doctors reach development agreement for Alzheimer's treatments
- 5 things you don't need to know but might want to
- Bizspace Property Spotlight: Do you see the sign? Crushed Red did
Sponsored by
People on the Move
SouthSide Early Childhood Center
SmithAmundsen LLC
Sara Investment Real Estate
Scheffel Boyle
Coldwell Banker Gundaker
Casey & Devoti, P.C.
CEO/President Kentucky Derby Museum | Louisville, KY
Program Director, Finance Lindenwood University | Saint Charles, MO
Real Estate Marketing Coordinator Anonymous | Saint Louis, MO
Program Director, Accounting Lindenwood University | Saint Charles, MO
Analyst or Associate, Asset Management Gramercy Property Trust | Saint Louis, MO
Featured Property
$93 per sqft
For Sale
$93 per sqft
For Sale


text: LinkedIn Recruiting Jobs 103 were found based on your criteria Post a job. It's free! Newest Jobs First Oldest Jobs First Most Employer Hours Least Employer Hours Most Dollars Paid Least Dollars Paid Highest Feedback Score Lowest Feedback Score 1 2 3 4 5 … › » Virtual Talent Sourcer - Technical/IT Fixed-Price – Est. Budget: $100.00 Posted 7 hours, 37 minutes ago Our recruiting consulting firm is seeking a part time sourcer to join our team. Are you looking to work in an early stage startup where you can rapidly advance your career and develop new skills? Are you creative in your research methods, and experienced in sourcing for hard to find top technical talent? Can you cope with rapidly changing requirements? RESPONSIBILITIES: Sourcing passive candidates through complex internet searches. While LinkedIn will be a key resource, we will also want you ... data-mining google-searching internet-research linkedin-recruiting technical-recruiter IT Recruitment Prospect for the state of Alabama Fixed-Price – Est. Budget: $600.00 Posted 10 hours, 34 minutes ago We are searching for talented and proactive freelancers who would like to participate in an exciting IT recruitment prospect for the state of Alabama. The goal of this project is to prospect technical people (engineers and project managers) who would like to work in Birmingham. The scope of the location to find candidates can be wider: Georgia, Atlanta and Louisiana. The profiles are very generic and are focused on 7 main technologies and 4 levels of seniority. Levels: - Java - .NET ... linkedin-recruiting market-research recruiting technical-recruiter Technical Sourcer/Recruiter Hourly – 1 to 3 months – 30+ hrs/week – Posted 1 day, 3 hours ago As we continue to solve the problem of web drudgery associated with online password management, it is crucial to have a world-class engineering team engaged in the process. Dashlane is growing rapidly, and we are looking for someone with hustle to help us grow our engineering team based in Paris and source/recruit the best engineers across Europe. +Create robust pipelines and have a knack for scanning the tech-ecosystem across Europe, +You are well versed with the latest technical hacks ... data-entry email-handling english english-grammar internet-research lead-generation linkedin-recruiting recruiting sales-writing writing Find names of decision makers in Californian engineering companies. Fixed-Price – Est. Budget: $100.00 Posted 1 day, 7 hours ago 1) I will give you a list of criteria for the type of engineering company you are looking for. 2) You will use google, facebook, linkdin, maps, yellowbook and whatever else you can, to find the names of the Managers and HR people in these companies. Anyone that may be involved in the hiring process of a specific department. 3) You will create a spreadsheet with the columns: Name, City, Company Name, Position, Company address, Contact Details (Whenever they are ... internet-research linkedin-recruiting Database Researcher Fixed-Price – Est. Budget: $50.00 Posted 1 day, 11 hours ago About Fixtrade LTD Fixtrade is a Sales Outsourcing company specialising in ICT (Information communications technology) for a wide range of high budget market sectors such as Financial, Telecom, Government and large enterprise. Fixtrade is a UK based company offering global services. Our team is growing rapidly and is based in the UK and in South Africa, with contractors in Europe, Asia and South Africa. Fixtrade's customers are predominantly based in Europe, Africa, Asia and the Middle East and the ... data-entry google-docs internet-research linkedin-recruiting microsoft-excel research Create a linkedIN profile Hourly – Less than 1 week – Less than 10 hrs/week – Posted March 25 2015 I am seeking someone who is knowledgable about Linkedin and is able to create a profile based on a resume. Integral knowledge of Linkedin is essential. The successful applicant will be able to work independently with no orientation to the Linkedin site and is able to return the work within 48 hours. Spelling, grammar and punctuation should be impeccable. linkedin-api linkedin-recruiting social-networking-development IT Recruiter / Talent Acquisition Specialist Hourly – 3 to 6 months – Less than 10 hrs/week – Posted March 25 2015 We are a 30+ people technology company in Atlanta, GA, looking to have more control over our talent acquisition process - and we would like you to help! We've hired 5-6 people every year in the past 4 and plan to do more in the future. We are looking for an experienced recruiter to work with passive talent. We will do most of the sourcing (although if you can source we would definitely consider your candidates too). Your responsibilities would ... hr-benefits human-resource-management linkedin-recruiting recruiting RECRUITER NEEDED IMMEDIATELY Fixed-Price – Est. Budget: $250.00 Posted March 25 2015 I need a recruiter to help me recruit Loan Officers for a mortgage company. YOU NEED TO HAVE COMPLETE CONTROL OF THE ENGLISH LANGUAGE FOR THIS POSITION. NEUTRAL ACCENTS ONLY I will need you to sort through the emails of the applicants and find the most qualified people. You'll then speak with them and schedule a time for the Loan Officer to speak with me and then I'll schedule them to come down to our office for an ... linkedin-recruiting recruiting Linkedin Expert Needed Hourly – 3 to 6 months – Less than 10 hrs/week – Posted March 25 2015 Hello, I would like to hire someone to manage my LinkedIn connections. My company sells laundry machinery to hotels, Laundromats , nursing homes, hospitals etc. Below, you will find several "types of people" that I would like to Link with. I am looking for someone that has experience in using LinkedIn, has experience in connecting with new connections on LinkedIn and can help market my company through LinkedIn. These are examples of people I would like to Connect with to give ... direct-marketing email-marketing facebook-marketing linkedin-recruiting trade-marketing Resume Rewriten Hourly – Less than 1 week – Less than 10 hrs/week – Posted March 25 2015 I am in need of a professional in writing and editing resumes to update mine. I would prefer someone with experience writing IT resumes If you are available please let me know. Also if you have a portfolio of any resume you have completed please send it to me. content-writing cover-letter-writing linkedin-recruiting recruiting resume-writing


text:
Dr. Glenn A. Mackay, DO
Infectious Disease
- 7255 Renner Rd
Shawnee, KS 66217
- 2 Locations
- (913) 962-6600
Dr. Glenn A. Mackay graduated from the Medical University of South Carolina College of Medicine in 1995. He works in Shawnee, KS and 1 other location and specializes in Infectious Disease. Dr. Mackay is affiliated with Olathe Medical Center, Overland Park Regional Medical Center, Providence Medical Center and Shawnee Mission Medical Center Inc. He speaks English and Spanish.
Gender: Male
Languages Spoken: English, Spanish
Infectious Disease
Medical School: Medical University of South Carolina College of Medicine; Graduated 1995
*This physician has multiple office locations. See Office Information for details.


text:
Current work programme
The annual work programme describes and summarises the main objectives, priorities and deliverables of the EBA in the forthcoming year. It is based on the tasks specified in the Regulation and in the relevant EU banking sector legislation.
Highlights of the EBA's tasks in 2015
In 2015, the EBA's activities will focus on three core areas: regulation, oversight and consumer protection.
In the regulatory policy area, the EBA will continue to play a central role in the development of the Single Rulebook in banking, which will contribute to the achievement of a level playing field for financial institutions, as well as to raise the quality of financial regulation in the EU and the overall functioning of the EU Single Market. The EBA's regulatory work stems from the European Commission's legislation on capital requirements (the CRDIV/CRR package) and the recovery and resolution framework (the Bank Recovery and Resolution Directive and the revision of the Deposit-Guarantee Schemes Directive). In 2015 we will focus in particular on the recovery and resolution framework, the prudential areas of liquidity and leverage, along with other regulatory tasks such as the EBA's contribution to the legislative processes.
The EBA's oversight activities will focus on identifying, analysing and addressing key risks in the EU banking sector. The EBA will continue to monitor capital levels and banks' capital plans to strengthen their capital position further. The EBA's work in the colleges of supervisors will continue to ensure effective European cross-border supervision and will include, where appropriate, binding mediation.
Finally, in the area of consumer protection, the EBA is fully committed to promote transparency, simplicity and fairness in the market for consumer financial products or services across the single market. The EBA will work towards fulfilling its mandates as laid down under the Mortgage Credit Directive, the proposed review of the Payment Services Directive, the Payment Accounts Directive and the Markets in Financial Instruments Regulation.
In addition, the EBA will continue to undertake independent impact assessment, perform peer reviews and ensure internal and external policy coordination between the EBA's core functions and external stakeholders. The EBA's legal team will provide legal review of the EBA's policy proposals, as well as assistance in the EBA's peer review work. The EBA staff will continue to play a critical role in supporting the EBA's core functions.
A detailed list of the EBA's tasks is presented in the Annex.


text:
cucumber question
I grew Japanese Climbing Cucumbers from SSE last year. I saved some seeds and planned on growing them again this year. But I am also thinking about pickling a few which I have never done.
So, should I also grow another type for pickling, or find one type that is okay both for salads and pickling? I am assuming that the Japanese Climbing isn't good for pickling. I had wanted to save seeds again this coming year, but wouldn't be able to if I grow 2 different types, right?
Any suggestions? I am in Wisconsin.
What kind of pickling do you plan to do?
I grow Poona Kheera last year and it has a permanent place from now on in my garden. It is a dual purpose cucumber, I made pickle relish with them and it turned out very good. The Poona Kheera did very well in my 4b/5a zone and has some disease resistance. I loved the flavor as it was never bitter. Note the color turns some people off, but it stopped people in the alley from stealing them.
"I had wanted to save seeds again this coming year, but wouldn't be able to if I grow 2 different types, right?" Yes, you can, IF you take measures to prevent the two types cross pollinating. That would be to bag some blooms before they open (some of both male and female ~ cover with lightweight paper bags or make some reusable drawstring ones out of floating row cover ~ make sure to seal the openings well when you put them on the blooms), take the bags off to hand pollinate once they open, then bag them immediately afterwards to keep bees out. Alternatively, you could "cage" them ~ simply cover the entire plant with something to exclude pollinators. This can be as simple as floating row cover (make sure to seal the edges well) or a bottomless box made of some sort of frame (wood or pvc pipe) and covered with window screening or floating row cover. Either way you do it, just make sure to save seeds only from blooms that have not been uncovered at all when open. And mark the ones you've hand pollinated ~ it's a real bummer when you don't do that (says the voice of experience ;).


text:
Was £25.00
Now £12.50
Now £7.50
70% Off
Save a total of £17.50
Grape floral short sleeved top
Item No.0570108133
This grape top from The Collection is printed with a floral design and fastens with buttons along the centre front. It has a v neck, short sleeves and an elasticated hem.
This item is available for
UK Home Delivery Free (UK orders over £30) or £3.99
Delivered within 4 working days
Next or Named Day Delivery
7 days a week between 8am - 6pm
Monday to Friday - order by 7pm - £4.99
Saturday & Sunday - order by 6pm - £4.99
Next or Named Evening Delivery
7 days a week between 6pm - 10pm
Monday to Sunday - order by 10pm - £6.99
Collect from store the next day - FREE
Order before 7pm for next day collection after 12pm midday
Show participating stores
Full Delivery details
- Button fastening
- Spare button supplied
- 100% viscose
- Machine washable
- Model wears: size 10
- Model Height: 175cm, 5'9", Bust: 32B, Waist: 24" , Hips: 34"


text:
Cut strawberries back to 3', can I plant pole beans?
I read that if I trimeed my strawberries back to about an 1" above crown ( I did 3"), it would increase my yield next yr. Can I now plant pole beans there? I figured since I am planting seeds, i wouldnt have to disturb the strawberry plants since I wont be digging. Any ideas?
If strawberries are June bearing yes.
If everbearing no, don't cut them back.
Depending on spacing of strawberries you can plant the beans in there, too. The plants need water & nutrients to set their berries for next year, so as long as you can provide what they both need, why not?
Be sure the trellis set up and when picking you replace any runners that get pulled up or sunken down.
I've grown garlic & onions between 2 rows of strawberries successfully.
I overplant my June bearing strawberries with butternut squash. Initially starting the squash in pots, I transplant the squash into the strawberry bed around the middle of June. Last year I had a great crop of both strawberries and squash in the same garden space!
Thanks a lot for the info. I just needed some confirmation this would work. My strawberry bed is on the end of my potager. I had planed on planting directly in with the strawberries, but since the strawberries had begun to run under the fence, so I tilled just outside this fence end, and ammended the soil. now i will go ahead and the let spill under the fence into this widened area. Now I am going to put the pole beans on the trellis I put over this end of the fence, but plant the pole beaNS at the edge of the berries on the outisde, then someething else at the feet. maybe more bush beans.
I grow butternut squash in our strawberry bed. They do really well and the strawberries seem to like the shade in July and August!


text:
The "Virtual File System" in Linux
The main data item in any Unix-like system is the "file", and a unique path name identifies each file within a running system. Every file appears like any other file in the way it is accessed and modified: the same system calls and the same user commands apply to every file. This applies independently of both the physical medium that holds information and the way information is laid out on the medium. Abstraction from the physical storage of information is accomplished by dispatching data transfer to different device drivers. Abstraction from the information layout is obtained in Linux through the VFS implementation.
Linux looks at its file system in the same way Unix does—adopting the concepts of super block, inode, directory and file. The tree of files accessible at any time is determined by how the different parts are assembled, each part being a partition of the hard drive or other physical storage device that is "mounted" to the system.
While the reader is assumed to be well acquainted with the concept of mounting a file system, I'll detail the concepts of super block, inode, directory and file.
The super block owes its name to its heritage, from when the first data block of a disk or partition was used to hold meta information about the partition itself. The super block is now detached from the concept of data block, but it still contains information about each mounted file system. The actual data structure in Linux is called struct super_block and holds various housekeeping information, like mount flags, mount time and device block size. The 2.0 kernel keeps a static array of such structures to handle up to 64 mounted file systems.
An inode is associated with each file. Such an "index node" holds all the information about a named file except its name and its actual data. The owner, group, permissions and access times for a file are stored in its inode, as well as the size of the data it holds, the number of links and other information. The idea of detaching file information from file name and data is what allows the implementation of hard-links—and the use of "dot" and "dot-dot" notations for directories without any need to treat them specially. An inode is described in the kernel by a struct inode.
The directory is a file that associates inodes to file names. The kernel has no special data structure to represent a directory, which is treated like a normal file in most situations. Functions specific to each file system type are used to read and modify the contents of a directory independently of the actual layout of its data.
The file itself is associated with an inode. Usually files are data areas, but they can also be directories, devices, fifos (first-in-first-out) or sockets. An "open file" is described in the Linux kernel by a struct file item; the structure holds a pointer to the inode representing the file. file structures are created by system calls like open, pipe and socket, and are shared by father and child across fork.
While the previous list describes the theoretical organization of information, an operating system must be able to deal with different ways to layout information on disk. While it is theoretically possible to look for an optimum layout of information on disks and use it for every disk partition, most computer users need to access all of their hard drives without reformatting, to mount NFS volumes across the network, and to sometimes even access those funny CD-ROMs and floppy disks whose file names can't exceed 8+3 characters.
The problem of handling different data formats in a transparent way has been addressed by making super blocks, inodes and files into "objects"; an object declares a set of operations that must be used to deal with it. The kernel won't be stuck into big switch statements to be able to access the different physical layouts of data, and new file system types can be added and removed at run time.
The entire VFS idea, therefore, is implemented around sets of operations to act on the objects. Each object includes a structure declaring its own operations, and most operations receive a pointer to the "self" object as the first argument, thus allowing modification of the object itself.
In practice, a super block structure encloses a field struct super_operations *s_op, an inode encloses struct inode_operations *i_op and a file encloses struct file_operations *f_op.
All the data handling and buffering performed by the Linux kernel is independent of the actual format of the stored data. Every communication with the storage medium passes through one of the operations structures. The file system type, then, is the software module which is in charge of mapping the operations to the actual storage mechanism—either a block device, a network connection (NFS) or virtually any other means of storing and retrieving data. These modules can either be linked to the kernel being booted or compiled as loadable modules.
The current implementation of Linux allows use of loadable modules for all file system types but root (the root file system must be mounted before loading a module from it). Actually, the initrd machinery allows loading of a module before mounting the root file system, but this technique is usually exploited only on installation floppies.
In this article I use the phrase "file system module" to refer either to a loadable module or a file system decoder linked to the kernel.
This is in summary how all file handling happens for any given file system type, and is depicted in Figure 1:
struct file_system_type is a structure that declares only its own name and a read_super function. At mount time, the function is passed information about the storage medium being mounted and is asked to fill a super block structure, as well as loading the inode of the root directory of the file system as sb->s_mounted (where sb is the super-block just filled). The additional field requires_dev is used by the file system type to state whether it will access a block device: for example, the NFS and proc types don't require a device, while ext2 and iso9660 do. After the super block is filled, struct file_system_type is not used any more; only the super block just filled will hold a pointer to it in order to be able to give back status information to the user (/proc/mounts is an example of such information). The structure is shown in Listing 1.
The super_operations structure is used by the kernel to read and write inodes, write super block information back to disk and collect statistics (to deal with the statfs and fstatfs system calls). When a file system is eventually unmounted, the put_super operation is called—in standard kernel wording "get" means "allocate and fill", "read" means "fill" and "put" means "release". The super_operations declared by each file system type are shown in Listing 2.
After a memory copy of the inode has been created, the kernel will act on it using its own operations. struct inode_operations is the second set of operations declared by file system modules, and is listed below; they deal mainly with the directory tree. Directory-handling operations are part of the inode operations because the implementation of a dir_operations structure would bring in extra conditionals in file system access. Instead, inode operations that only make sense for directories will do their own error checking. The first field of the inode operations defines the file operations for regular files. If the inode is a fifo, a socket or a device-specific file operation will be used. Inode operations appear in Listing 3; note the definition of rename was changed in release 2.0.1.
The file_operations, finally, specify how data in the actual file is handled: the operations implement the low-level details of read, write, lseek and the other data-handling system calls. Since the same file_operations structure is used to act on devices, it also includes some fields that only make sense for character or block devices. It's interesting to note that the structure shown here is the structure declared in the 2.0 kernels, while 2.1 changed the prototypes of read, write and lseek to allow a wider range of file offsets. The file operations (as of 2.0) are shown in Listing 4.
great info, but having problems to build a module
I found the information about /proc because I am looking to interact with some hardware with GPL release code. Great introduction, thanks.
The only problem I have is that in my test module code, when I build it, proc_register_dynamic is not found.
I am clueless, working on MIPS based system, kernel version 2.4.19.
It's a wireless device being sold by Linksys.
- What continuous engineering is
- How to continuously improve complex product designs
- How to anticipate and respond to markets and clients
- How to get the most out of your engineering resources
Sponsored by IBM
Learn to:
- Define service virtualization
- Select the most beneficial services to virtualize
- Improve your traditional approach to testing
- Deliver higher-quality software faster
Sponsored by IBM
Trending Topics
Webinar: 8 Signs You're Beyond Cron
11am CDT, April 29th
Join Linux Journal and Pat Cameron, Director of Automation Technology at HelpSystems, as they discuss the eight primary advantages of moving beyond cron job scheduling. In this webinar, you'll learn about integrating cron with an enterprise scheduler.Join us!
|Not So Dynamic Updates
|Mar 30, 2015
|Users, Permissions and Multitenant Sites
|Mar 26, 2015
|New Products
|Mar 24, 2015
|Flexible Access Control with Squid Proxy
|Mar 23, 2015
|Solving ODEs on Linux
|Mar 19, 2015
|DevOps: Everything You Need to Know
|Mar 19, 2015
- New Products
- Users, Permissions and Multitenant Sites
- Flexible Access Control with Squid Proxy
- Security in Three Ds: Detect, Decide and Deny
- Not So Dynamic Updates
- High-Availability Storage with HA-LVM
- Tighten Up SSH
- DevOps: Everything You Need to Know
- Solving ODEs on Linux
- Non-Linux FOSS: MenuMeters


text:
Lindsey Vuolo…UH YUMP!!!
One of my all time favorite Playboy Playmates WOW!!!!!!
I'd luv to give her a big hug, so we can bump boobies together… Double-D's baby!
Wolfendale's salutes Lindsey
I like your reply and I'm assuming much like Miss Vuolo that you are STACKED!
Dennis Rog
Who's that blond hottie in the second picture?
Simply gorgeous! And NOTHING less than totally HOT!!!
You're in point of fact a excellent webmaster. The website loading speed is amazing. It sort of feels that you're doing any distinctive trick. In addition, The contents are masterpiece. you've done a great activity on this subject!


text:
I'm having a bit of a nightmare getting a crontab/cronjob to run an Artisan command.
I have another Artisan command running via cronjob no problems but this second command won't run.
Firstly, when I do 'crontab -e' and edit the file to contain:
0 0 * * * /usr/local/bin/php /home/purple/public_html/artisan feeds:send
The cronjob doesn't run at all.
If I go to cPanel and add the cronjob there, it runs but I receive the following error:
open(public/downloads/feeds/events.csv): failed to open stream: No such file or directory
The thing is the file exists and the directories have the correct permissions. If I run the command when logged in via SSH as root or the user purple (php artisan feeds:send) the command runs flawlessly and completes its tasks no problem.
If in cPanel, I edit the cronjob to use:
0 0 * * * php /home/purple/public_html/artisan feeds:send
I receive the following error:
There are no commands defined in the "feeds" namespace.
The funny thing is that my other command is registered in the crontab file and works and has no reference in cPanel at all.
Any help would be much appreciated. Just for brevity I have included the command and model that the command uses.
Feed.php Model:
DataFeedController.php Controller:
SendFeeds.php Command:
FeedInterface.php Interface:
As you can see there is a GetRates command, which works.


text:
1. Using a food processor blend the sweet potatoes, banana, egg yolk, salt and cream until smooth or blender.
2. Into a bowl pour and fold in the egg white with a metal spoon.
3. Onto a greased baking sheet add spoonfuls of the mixture and let it bake in a preheated 500 F/250 C oven for 6-8 minutes.
4. Serve sprinkled with a little sugar and grated nutmeg.


text:
#50 of 60
henryk greeting cards, gorecki greeting cards, oil greeting cards, on greeting cards, canvas greeting cards, construktors greeting cards, of greeting cards, time greeting cards, surrealism greeting cards, fantasy greeting cards, sun greeting cards
,, Construktors of Time,,;painting oil on canvas;50/70 cm.1992 y.What is time?By definition,a physical quantity for the order of events.According to some theories is the ilusion of size and imaginary.To be completed by all and always shortage.Waiting-stretches like rubber.Difficult to manage feature reality!Who is responsible for this?For peace of mind I painted ,,Constructors of Time,,...Of course,I know-black holes,the Bing-Bang...no painting!It is so interesting and you can choose who is to blame for our problems in time...Personally,I do not know! --DISTINCTIONS IN GROUPS: -23.02.15-Created by my Hands; -15.02.15-Geek Art; -26.08.14-Imagination; -10.04..14-The Light Painters; -2.11.13-Artist Best Five Artwork; -2.11.13-Visual...
I was born in 1947 in Poland.Currently i am retired,but in the past worked in a variety of occupations,rather far from art.First year career he was associated with military aviation as a mechanic Flight.Despite the passage of time still walk with your in the clouds.I liked the change and I was curious about the world.I worked in a coal mine,a car factory,in the forest,in the wood and gas station.Also as a longshoreman,builder,restorer bridges etc.Constant in my life turned out to be a passion for fusing reality with the help of various types of media.After the children s crayons stage in the 60-s I discovered for myself the technique oil.This fascination does not pass.Officially did not recaive any science in the field of painting.I am a...
Our return policy is very simple:
If you're not happy with a purchase that you made on FineArtAmerica.com, for any reason, you can return it to us within 30 days of the order date. As soon as it arrives, we'll issue a full refund for the entire purchase price. Please note - Fine Art America does not reimburse the outgoing or return shipping charges unless the return is due to a defect in quality.
Fine Art America sells thousands of pieces of artwork each month - all with a 100% money-back guarantee.
If you've hesitated to purchase artwork online in the past, why not give us a try? You have nothing to lose!
We take great pride in the fact that artists have chosen Fine Art America to fulfill their orders, and we look forward to helping you select your next piece!
Customer Service: 877-807-5901
(Call Any Time - 24 Hours Per Day)
All greeting cards ship from our production facility within two business days of your order.
Beverly Hills, CA (United States)
Sign up for our newsletter for exclusive deals, discount codes, and more:
Unsubscribe at any time.
Server Status OK
Copyright © 2015 FineArtAmerica.com - All Rights Reserved


text:
Participation resources for local authorities
On this page you will find resources to support your public awareness work on encouraging voter registration and providing information on elections.
These resources – for use by electoral administrators and communication teams – include advertising templates, press releases and practical materials to encourage public participation in the electoral process.
If you have any queries, please email firstname.lastname@example.com.
Promoting voter registration
Here you will find general resources for promoting voter registration. These include templates for posters, information leaflets and guidance on how to register to vote.
UK general election 2015
You can't vote unless you've registered
You can use the resources below to help get people registered to vote in time for the UK general election on 7 May.
Poster / press
- 'You can't vote unless you've registered' poster – English (PDF)
- 'You can't vote unless you've registered' poster – Welsh bilingual (PDF)
- 'You can't vote unless you've registered' poster – Northern Ireland (PDF)
Website banners
Click here to view other resources for promoting voter registration
National Voter Registration Day - 5 February
These resources were developed for National Voter Registration Day on 5 Feb 2015 but you are welcome to amend them so that they can be used in the run-up to the UK general election.
- Template NVRD press release for local authorities in England (DOC)
- Template NVRD press release for local authorities in Scotland(DOC)
- Template NVRD press release for local authorities in Wales (DOC)
- Template NVRD press release for local authorities in Wales - Welsh version (DOC)
- NVRD suggested blog - English and Welsh (DOC)
- Social media – Facebook and Twitter – posts for NVRD in English and Welsh
Providing voter information
Here you will find general resources for providing information on voting and election processes.
Click here to view resources for providing voter information
Voter materials guidance – Making your mark
Here you will find best practice guidance and example materials for using when designing voter materials, including ballot papers, guidance notices in polling stations and polling booths, postal voting materials and bilingual materials.
Click here to view the full list of Making your mark resources
Full guidance
- Making your mark: design guidance for electoral administrators (PDF)
- Making your mark: design guidance for government policy-makers (PDF)
- Making your mark: project summary (PDF)
Guidance by section
Example materials
Postal voting quick start guides
Postal voting statements
|Example postal voting statement - GB English A4 (PDF)
|Example postal voting statement - GB bilingual A4 (PDF)
|Example declaration of identity Northern Ireland - English A4 (PDF)
Order hard copies and download images
Order hard copies
You can order hard copies of our service voters registration pack completely free. Phone our orderline on 0845 8500 501, or order materials by email.
Download images
We also have a selection of images that you can use, which are available on our flickr site.


text:
Intel Quad-Core Price and Performance Showdown 115
from the horses-for-courses dept.
We've improved Slashdot's video section; now you can view our video interviews, product close-ups and site visits with all the usual Slashdot options to comment, share, etc. No more walled garden! It's a work in progress -- we hope you'll check it out (Learn more about the recent updates).
What is the responsiveness of the system under load? Openssl speed? bonnie++?
Nobody in their right mind should get an i7. A 30% performance-per-clock increase over the Core 2 series is not worth doubling the cost of the CPU and motherboard. DDR3 is also more expensive than DDR2. On top of that, Intel are getting into the power-sucking height of the Pentium 4s again; the Core i7s have a TDP of 130 watts. For any desktop use - including highest-end game machines - anything another other than a Core 2 Duo is just a waste of money.
i've got:
amd 64 x2 at 25W (BE-2350)
690G motherboard with onboard graphics (ASUS M2A-VM HDMI)
$165 total in dec 2007
and it runs fanless fine (tho i do have the fan hooked up idling and thermally controlled most of the time). doesn't look like they're still selling the BE-2350, and not sure if there's a current equivalent or if you can accomplish the same thing by underclocking.
% APL is a natural extension of assembler language programming; ...and is best for educational purposes. -- A. Perlis


text:
NANOShorts Entertainment is $10,000 Winner of PaintCare Video Contest
When Civilian set out to hire professional comedy sketch gro
We are Civilian. We're glad you could join us. Civilian is an independent agency working exclusively with clients who seek to strengthen communities. We know that sounds a little odd coming from someone in the marketing, advertising, and communications industry. But it's who we are, and it's the work we're really good at. Not sure if that category includes you? Well, if what you're trying to accomplish matters, if what you're working toward is difficult to quantify but easy to observe, or if your mission centers on improving the lives of people, we're likely the partner for you. In fact, if you're simply not sure… give us a shout. Let's create some meaningful change.
Harrison Boyd
Manager, Marketing Services
San Diego Padres
When Civilian set out to hire professional comedy sketch gro
Have you ever wondered what will happen to your social media
PaintCare, the innovative organization behind paint-recyclin


text:
Already a Member?
Log In
By clicking "Shop Now", you accept our
Terms of Use
Privacy Policy
Forgot Password?
Not a Member?
Join Now
Login with Facebook
Please enter your email address below and we will send you an email to confirm your password.
Thank you. If a matching email exists we will send you an email in a few minutes with instructions on how to reset your password.
Don't forget to check your spam folder if you cannot find the email. For further assistance please contact us at
Common Good
This bergamot hand and dish soap comes in two plastic bottles. Plant based, biodegradable and blended with 100-percent pure essential oil. No animal-derived ingredients or animal testing.
© 2015 One Kings Lane®, All rights reserved.
Terms of Use
Tastemaker Tag Sales™ is a trademark of One Kings Lane Inc.
One Kings Lane and the elephant logo are registered trademarks of One Kings Lane Inc.


text:
The GQ Bar in Dubai's JW Marriott is a fantastic place to meet friends for cocktails and conversation. It is also a dining pleasure with a sexy ambiance, passionate service, and scrumptious dishes with presentations that are nothing less than art.
Ziggy's is one of the most successful restaurants in Cappadocia -- a visit will show you why
Restauranteur Muti's creative energies drive him to develop new twists on authentic Anatolian cuisine
Keep it Sweet, Delicous and Organic with Nocciolata
There are certain restaurants that are such American and global classics that they're emblazoned on our consciousness. We think we know them inside and out. But stand back: the Hard Rock Cafe is reinventing itself beyond our wildest expectations
coconut oil is an inexpensive and fabulous remedy for dry hair and skin!
The World Cup is about to kickoff and while you probably aren't going to make it to Brazil, you can enjoy a little bit of nearly every participating country with a visit to Las Vegas. The Las Vegas strip can take you to Spain, England, France, Australia, Thailand and more
As someone who is both a vegetarian and gluten-free,I was very curious when I was sent information about an "Idiot's Guides as easy as it gets! - The Mediterranean Diet Cookbook" and wanted to try some recipes
The Country Legends perform at Uncle Darrow's annual Crawfish Boil on Saturday, June 14th from 2-5pm and all night (7-10 pm) at Dannys Venice on Wednesday, June 18th.
Are you wondering what to do for Father's Day, June 15th? Here are a few suggestions
Wine country does it again. Days of Wine and Lavender. June 28,2014. Event is held from 12 to 4 p.m. at 6097 Bennett Valley Road, Santa Rosa, California 95404.
Napa and the fine goods it is known for took center stage at BottleRock '14
Located in the heart of downtown Napa lies an extraordinary culinary experience, 1313 Main. Giving you the freshest ingredients and is the creme de la creme of innovative yet distinctive dining
A steakhouse, when it comes down to it is judged by the steak it serves. The first cut of that steak and then the first bite, if you are a steak aficionado that will define your entire experience. Chianina in Long Beach has such confidence in its steak that they don't even have steak knives. Such a perfect cut of beef, a regular knife will more than do and that first bite, well, it will leave you speechless
The Greenhouse Cafe came into view right next to the Mortenson Bakery. This cafe is actually inside of a real greenhouse, which has been converted into a restaurant
Best Food & Beverage gifts for Father's Day 2014


text:
Population Genetics Lab 2 BINOMIAL PROBABILITY & HARDY-WEINBERG EQUILIBRIUM Last Week : Sample Point Methods: Example: Use the Sample Point Method to find the probability of getting exactly two heads in three tosses of a balanced coin. 1. The sample space of this experiment is: Outcome Toss 1 Toss 2 Toss 3 Shorthand Probabilities 1 Head Head Head HHH 1/8 2 Head Head Tail HHT 1/8 3 Head Tail Head HTH 1/8 4 Tail Head Head THH 1/8 5 Tail Tail Head TTH 1/8 6 Tail Head Tail THT 1/8 7 Head Tail Tail HTT 1/8 8 Tail Tail Tail TTT 1/8 2. Assuming that the coin is fair, each of these 8 outcomes has a probability of 1/8. 3. The probability of getting two heads is the sum of the probabilities of outcomes 2, 3, and 4 (HHT, HTH, and THH), or 1/8 + 1/8 + 1/8 = 3/8 = 0.375. Sample- point method : Example: Find the probability of getting exactly 10 heads in 30 tosses of a balanced coin. Total # of sample points = 230 = 1,073,741,824 Need a way of accounting for all the possibilities Example: In drawing 3 M&Ms from an unlimited M&M bowl that is always 60% red and 40% green, what is the P(2 green)? If one green M&M is just as good as another… Binomial Probability Distribution Where, n = Total # of trials. y = Total # of successes. s = probability of getting success in a single trial. f = probability of getting failure in a single trial (f = 1-s). Assumptions of Binomial Distribution 1. # of trials are independent, finite, and conducted under the same conditions. 2. There are only two types of outcome.(Ex. success and failure). 3. Outcomes are mutually exclusive and independent. 4. Probability of getting a success in a single trial remains constant throughout all the trials. 5. Probability of getting a failure in a single trial remains constant throughout all the trials. 6. # of success are finite and a non-negative integer (0,n) Properties of Binomial Distribution Mean or expected # of successes in n trials, E(y) = ns Variance of y, V(y) = nsf Standard deviation of y, σ (y) = (nsf)1/2 Example: Find the probability of getting exactly 10 heads in 30 tosses of a balanced coin. Solution: We know, n = 30 y = 10 s = 0.5 f = 0.5 Example: Find the expected # of heads in 30 tosses of a balanced coin. Also calculate variance. Solution: E(Y) = ns = 30*0.5 = 15 V(Y) = nsf = 30*0.5*0.5 = 7.5 Problem 1 (10 minutes)(2 points) An allozyme locus has three alleles, A1,A2, and A3 with frequencies 0.947, 0.033, and 0.020, respectively. If we sample 30 diploid individuals, what is the probability of: •Not finding any copies of A2? •Finding at least one copy of A2? •GRADUATE STUDENTS ONLY: Finding fewer than 2 copies of A2? Example: How many diploid individuals should be sampled to detect at least one copy of allele A2 from Problem 1 with probability of at least 0.95? Solutions: Thus, to detect at least one copy of allele A2 with probability of 0.95, one would need to sample at least 90 alleles (i.e., at least 45 diploid individuals). Problem 2 (15 minutes)(2 points) The frequency of red-green color-blindness is 0.07 for men and 0.005 for women. Cities are considering implementing new traffic lights that can be distinguished by people with this disability. However, the investment is large, so President Obama has asked you to calculate the minimum size of a city that would have a probability of 0.9 of having at least 10 men who are red-green color-blind. Assume equal population sizes for men and women. Full credit (2 pts): Solve for at least 1 man +1 Bonus: Set up the equation for at least 10 men +3 Bonus: Solve for at least 10 men Estimation of allele frequency for Co-dominant locus Where, p = Frequency of allele A1 q = Frequency of Allele A2 N11 = # of individuals with genotype A1A1 N12 = # of individuals with genotype A1A2 N22 = # of individuals with genotype A2A2 N = total # of diploid individuals =N11+N12+N22 Estimation of Standard Error Where, p = Frequency of allele A1 q = Frequency of Allele A2 SEp = Standard error for frequency of allele A1 SEq = Standard error for frequency of allele A2 N = total # of diploid individuals =N11+N12+N22 Standard Deviation v. Standard Error We expect ~68% of the data to fall within 1 standard deviation of the mean. Standard error is the standard deviation of the mean. We expect sample means to fall within 1 standard error of the true mean ~68% of the time. Example: What are the allele frequencies of alleles A1 and A2, if the following genotypes have been observed in a sample of 50 diploid individuals? Genotype Count A1A1 17 A1A2 23 A2A2 10 Solution: N11 = 17, N12 = 23, and N22 = 10 q = 1 – p = 0.43 Problem 3 (10 minutes) (2 pts) Estimate the allele frequencies (include their respective standard errors) for alleles A1, A2, and A3 if the following genotypes have been observed in a sample of 200 individuals Genotype Count A1A1 19 A2A2 17 A3A3 14 A1A2 52 A1A3 57 A2A3 41 Problem 4 (Time 10 min.)(2 pts) Tay Sachs disease is an autosomal recessive genetic disorder causing the death of nerve cells in the brain due to the steady accumulation of gangliosides. Extensive genotyping has determined that approximately 1 in 30 of the 5 million Ashkenazi Jews within the United States is a carrier. a) Assuming HWE and Mendelian inheritance of the disease, what is the frequency of the recessive allele in this population? b) What is the SE of this estimate? (Assume 1,000 people were sampled) c) How many affected children would you expect to be born in this population? d) What are the assumptions of these estimates? Hypothesis Testing Hypothesis: Tentative statement for a scientific problem, that can be tested by further investigations. 1.Null Hypothesis(Ho): There is no significant difference in observed and expected values. 2.Alternate Hypothesis(H1): There is a significant difference in observed and expected values. Example: Ho = Fertilized and unfertilized crops have equal yields H1 = Fertilized and unfertilized crops do not have equal yields Remember: In final conclusion after the experiment ,we either – "Reject H0 in favor of H1" Or "Fail to reject H0", Type I error: Error due to rejection of a null hypothesis, when it is actually true (False positive). Level of significance(LOS) (α) : Maximum probability allowed for committing "type I error". At 5 % LOS (α=0.05), we accept that if we were to repeat the experiment many times, we would falsely reject the null hypothesis 5% of the time. P- value: Probability of committing type I error If P-value is smaller than a particular value of α, then result is significant at that level of significance Testing departure from HWE In a randomly mating population, allele and genotype frequencies remain constant from generation to generation. Ho= There is no significant difference between observed and expected genotype frequencies (i.e. Population is in HWE) H1= There is a significant difference between observed and expected genotype frequencies (i.e. Population is not in HWE) HWE Assumptions 1. Random mating 2. No selection a. Equal numbers of offspring per parent b. All progeny equally fit 3. No mutation 4. Single, very large population 5. No migration χ2 - test Where, Example: A population of Mountain Laurel at Cooper's Rock State Forest has the following observed genotype counts: Genotype Observed number A1A1 5000 A1A2 3000 A2A2 2000 Is this population in Hardy-Weinberg equilibrium ? Genotype Expected frequency Expected number under HWE under HWE A1A1 p2 = 0.652 = 0.4225 0.4225 10000 = 4225 A1A2 2pq = 0.455 0.455 10000 = 4550 A2A2 q2 = 0.1225 0.1225 10000 = 1225 Genotype Obs. #(O) Exp. #(E) (O-E) (O-E)^2 (O-E)^2/E A1A1 5000 4225 775 600625 142.1598 A1A2 3000 4550 -1550 2402500 528.022 A2A2 2000 1225 775 600625 490.3061 χ2 1160.488 The critical value (Table value) of χ2 at 1 df and at α=0.05 is approx. 3.84. Conclusion: Because the calculated value of χ2 (1160.49) is greater than the critical value (3.84), we reject the null hypothesis and accept the alternative (Not in HWE). Problem 5 (Time 10 min) (2 pts) Based on the observed genotype counts in problem 3, test whether the population that had been sampled is in HWE. What are some possible explanations for the observed results? Genotype Count A1A1 19 A2A2 17 A3A3 14 A1A2 52 A1A3 57 A2A3 41
Pages to are hidden for
"Slide 1 - West Virginia Universi"Please download to view full document