text:
Page 1
Large-scale fine mapping of the HNF1B locus and
prostate cancer risk
Sonja I.Berndt1,∗, JoshuaSampson1, MeredithYeager1,2, Kevin B.Jacobs3, ZhaomingWang1,2,
Amy Hutchinson1,2, Charles Chung1,2, Nick Orr1, Sholom Wacholder1, Nilanjan Chatterjee1,
Kai Yu1, Peter Kraft4, Heather Spencer Feigelson5, Michael J. Thun6, W. Ryan Diver6,
Demetrius Albanes1, Jarmo Virtamo7, Stephanie Weinstein1, Fredrick R. Schumacher8,
Geraldine Cancel-Tassin9, Olivier Cussenot9, Antoine Valeri9, Gerald L. Andriole10,
E. David Crawford11, Christopher Haiman8, Brian Henderson8, Laurence Kolonel12, Loic Le
Marchand12, Afshan Siddiq13, Elio Riboli13, Ruth C. Travis14, Rudolf Kaaks15, William Isaacs16,
Sarah Isaacs16, Kathleen E. Wiley16, Henrik Gronberg17, Fredrik Wiklund17, Pa ¨r Stattin18,
JianfengXu19, S. LillyZheng19, JielinSun19, Lars J.Vatten20, KristianHveem20, IngerNjølstad21,
Daniela S. Gerhard22, Margaret Tucker1, Richard B. Hayes23, Robert N. Hoover1,
Joseph F. Fraumeni Jr1, David J. Hunter24, Gilles Thomas1and Stephen J. Chanock1
1Division of Cancer Epidemiology and Genetics, Department of Health and Human Services, National Cancer
Institute, National Institutes of Health, Bethesda, MD, USA,2Core Genotyping Facility, Advanced Technology
Program, SAIC-Frederick Inc., NCI-Frederick, Frederick, MD, USA,3Bioinformed Consulting Services, Gaithersburg,
MD, USA,4Program in Molecular and Genetic Epidemiology, Department of Epidemiology, Harvard School of Public
Health, Boston, MA, USA,5Institute for Health Research, Kaiser Permanente, Denver, CO, USA,
6Department of Epidemiology and Surveillance Research, American Cancer Society, Atlanta, GA, USA,7Department
of Chronic Disease Prevention, National Institute for Health and Welfare, Helsinki, Finland,8Department of Preventive
Medicine, Keck School of Medicine, Los Angeles, CA, USA,9Centre de Recherche pour les Pathologies Prostatiques,
Ho ˆpital Tenon, Assistance Publique-Ho ˆpitaux de Paris, 75970 Paris, France,10Division of Urologic Surgery,
Washington University School of Medicine, St Louis, MO, USA,11Department of Surgery, University of Colorado at
Denver and Health Sciences Center, Denver, CO, USA,12Epidemiology Program, Cancer Research Center,
University of Hawaii, Honolulu, HI, USA,13Division of Epidemiology, Public Health and Primary Care, Imperial College
London, London, UK,14Cancer Epidemiology Unit, Nuffield Department of Clinical Medicine, University of Oxford,
Oxford, UK,15Division of Cancer Epidemiology, German Cancer Research Centre (DKFZ), Heidelberg, Germany,
16Department of Urology, Johns Hopkins Medical Institutions, Baltimore, MD, USA,17Department of Medical
Epidemiology and Biostatistics, CLINTEC, Karolinska Institute, Stockholm, Sweden,18Department of Surgical and
Perioperative Sciences, Urology and Andrology, Umea ˚ University, Umea ˚, Sweden,19Center for Cancer Genomics,
Wake Forest University School of Medicine, Winston-Salem, NC, USA,20Department of Public Health and General
Practice, Norwegian University of Science and Technology, Trondheim, Norway,21Institute of Community Medicine,
University of Tromsø, Tromsø, Norway,22Office of Cancer Genomics, Department of Health and Human Services,
National Cancer Institute, National Institutes of Health, Bethesda, MD, USA,23Department of Environmental Medicine,
New York University Medical Center, New York, NY, USA,24Channing Laboratory, Brigham and Women's Hospital,
Harvard Medical School, Boston, MA, USA
Received February 1, 2011; Revised April 14, 2011; Accepted May 9, 2011
∗To whom correspondence should be addressed at: Sonja Berndt, Division of Cancer Epidemiology and Genetics, National Cancer Institute,
6120 Executive Blvd, EPS 8116, MSC 7240, Bethesda, MD 20892-7240. Tel: +1 3015947898; Fax: +1 3014021819; Email: firstname.lastname@example.com
Published by Oxford University Press 2011.
Human Molecular Genetics, 2011, Vol. 20, No. 16
Advance Access published on May 16, 2011
Page 2
Previous genome-wide association studies have identified two independent variants in HNF1B as suscepti-
bility loci for prostate cancer risk. To fine-map common genetic variation in this region, we genotyped
79 single nucleotide polymorphisms (SNPs) in the 17q12 region harboring HNF1B in 10 272 prostate
cancer cases and 9123 controls of European ancestry from 10 case–control studies as part of the Cancer
Genetic Markers of Susceptibility (CGEMS) initiative. Ten SNPs were significantly related to prostate
cancer risk at a genome-wide significance level of P < 5 3 1028with the most significant association with
rs4430796 (P 5 1.62 3 10224). However, risk within this first locus was not entirely explained by rs4430796.
Although modestly correlated (r25 0.64), rs7405696 was also associated with risk (P 5 9.35 3 10223) even
after adjustment for rs4430769 (P 5 0.007). As expected, rs11649743 was related to prostate cancer risk
(P 5 3.54 3 1028); however, the association within this second locus was stronger for rs4794758
(P 5 4.95 3 10210), which explained all of the risk observed with rs11649743 when both SNPs were included
in the same model (P 5 0.32 for rs11649743; P 5 0.002 for rs4794758). Sequential conditional analyses indi-
cated that five SNPs (rs4430796, rs7405696, rs4794758, rs1016990 and rs3094509) together comprise the
best model for risk in this region. This study demonstrates a complex relationship between variants in the
HNF1B region and prostate cancer risk. Further studies are needed to investigate the biological basis of
the association of variants in 17q12 with prostate cancer.
Of all cancers, prostate cancer is one of the most heritable with
genetic factors estimated to account for 42% of the risk (1).
Genome-wide association studies (GWAS) have been highly
successful in discovering susceptibility loci for prostate
cancer and at least 30 loci have been identified to date
(2–15). One of the earliest loci to be discovered for prostate
cancer was a variant, rs4430796, in HNF1B at chromosome
17q12 in men of European background (6). In a subsequent
GWAS in Japanese men, the same locus was identified (15).
A second independent variant, rs11649743, located at chromo-
some 17q12 and separated by a recombination hotspot from
the first variant, was subsequently found to be associated
with risk (10). The HNF1B locus as well as two other prostate
cancer susceptibility loci, chromosome 7p15.2 (JAZF1) and
chromosome 2p21 (THADA), have also been shown to be
associated with diabetes risk (6,16). Although epidemiologic
studies have shown that diabetes is inversely associated with
prostate cancer (17), variants in HNF1B and JAZF1 do not
explain the association between diabetes and prostate cancer
The strongest variants associated with prostate cancer risk at
chromosome 17q12 localize to a region that harbors HNF1B, a
gene that encodes a POU homeodomain-containing transcrip-
tion factor. POU transcription factors help regulate the devel-
opment of neuroendocrine organs, and HNF1B has been
shown to play a regulatory role in nephron and pancreas devel-
opment (19,20). Rare mutations in HNF1B have been associ-
ated with maturity-onset diabetes of the young type 5
(MODY5), kidney disorders, pancreatic atrophy, and genital
malformations (21,22). Although the biological mechanism
by which HNF1B may affect prostate cancer risk has not
been elucidated, differential expression of HNF1B has been
associated with prostate cancer recurrence (23).
To further characterize genetic variation in the HNF1B
region and the risk of prostate cancer, we conducted a
large-scale fine mapping study using tag single nucleotide
polymorphisms (SNPs) based on HapMap data in 10 272
prostate cancer cases and 9123 controls of European ancestry
from 10 case–control studies as part of the Cancer Genetic
Markers of Susceptibility (CGEMS) initiative. A total of 79
SNPs in the HNF1B region that were genotyped and passed
quality control criteria were analyzed in this study.
We analyzed 79 SNPs located in a 249 kb region surrounding
HNF1B (chromosome 17: 33,010,707–33,259,778) in 10 272
prostate cancer cases and 9123 controls from 10 studies (Sup-
plementary Material, Table S1). Ten SNPs were significantly
associated with prostate cancer risk below the threshold of
genome-wide significance (P , 5 × 1028); the most signifi-
cant association observed was the previously identified SNP
rs4430796 (P ¼ 1.62 × 10224) (Table 1, Fig. 1A). Eight of
the 10 significant SNPs were located in the first HNF1B
region associated with prostate cancer, and all eight were
highly correlated in controls with D′≥ 0.6 and pairwise r2
values between 0.13 and 0.94. However, risk within this first
locus was not entirely explained by rs4430796. Although mod-
estly correlated (r2¼ 0.64), rs7405696 was also associated
with prostate cancer risk (P ¼ 9.35 × 10223). After condition-
ing on rs4430796, rs7405696 retained an association with risk
(P ¼ 0.007). None of the other SNPs in region 1 remained
associated with risk after adjustment for rs4430796 (Sup-
plementary Material, Table S2).
Two SNPs located in the second identified region by Sun
et al. (10) were also associated with the prostate cancer risk
at a significance level of 5 × 1028. As expected, the pre-
viously identified SNP in the second region, rs11649743,
was associated with prostate cancer risk (P ¼ 3.54 × 1028);
however, the association within this second locus was stronger
for rs4794758 (P ¼ 4.95 × 10210). The two SNPs were corre-
lated in controls (r2¼ 0.61), but when rs11649743 and
rs4794758 were included in the same model, only rs4794758
remained associated with risk (P ¼ 0.002 for rs4794758;
P ¼ 0.32 for rs11649743). Interestingly, it accounted for the
risk associated with rs11649743.
Human Molecular Genetics, 2011, Vol. 20, No. 163323
Page 3
To examine the interdependence of the signals observed on
chromosome 17q12, we first conducted a set of sequential con-
ditional analyses, conditioning on the most significant SNP
from the unconditional analysis and each conditional analysis
sequentially until no SNPs remain nominally associated with
risk (P , 0.05) (Supplementary Material, Table S2). After
conditioning on the most significant SNP (rs4430796 in
region 1), six SNPs remained nominally associated with risk
(P , 0.05) with the most significant SNP being rs4794758
in region 2 (Fig. 1B, Supplementary Material, Table S2).
Although rs4430796 and rs4794758 were separated by a
modest recombination hotspot, there was some correlation
between them (r2¼ 0.04) and consequently the P-value for
rs4794758 was attenuated after conditioning on rs4430796
(P ¼ 3.45 × 1025). After conditioning on both rs4430796
and rs4794758, four SNPs were nominally associated with
risk (P , 0.05) with the most significant SNP being
rs1016990 (P ¼ 0.009). This SNP was only nominally associ-
ated with risk in the unconditional model (P ¼ 0.0002) and not
significantly associated with risk after conditioning on
rs4430796 only. Further sequential conditional analyses
yielded rs7405696 (P ¼ 0.01) as the most significant SNP
after conditioning on rs4430796, rs4794758 and rs1016990,
followed by rs3094509 (P ¼ 0.02) after conditioning on
rs4430796, rs4794758, rs1016990 and rs74056969. No other
SNPs remained nominally associated with prostate cancer
risk after conditioning on these five SNPs, suggesting that
these SNPs(i.e. rs4430796,
rs74056969 and rs3094509) capture the risk in this region.
To ascertain whether the same SNPs were identified using
other statistical approaches, we performed forward stepwise
regression adding SNPs below a significance level of 0.05.
This method resulted in the inclusion of four SNPs:
rs4430796, rs4794758, rs1016990 and rs74056969 in the
model. Using lasso, five SNPs (rs4430796, rs2005705,
rs7405696, rs4794758 and rs11649743) entered the model at
a lambda .0.01. A comparison of the models selected by
these three methods using Akaike information criterion
(AIC) indicated that the SNPs using the sequential conditional
analysis method yielded the best model (Table 2). The sequen-
tial conditional model was also found to be a better model than
the model containing the most significant SNP from region 1
and region 2 (AIC: 25351.114 versus 25364.42 for the sequen-
tial and two SNP models, respectively). We imputed the SNPs
from this region available from the 1000 Genomes Project and
conducted a sequential conditional analysis with the imputed
data. The results were quite similar with at least four of the
five SNPs either being the same SNP or a highly correlated
SNP (r2. 0.95) as observed in our sequential conditional
analysis with directly genotyped SNPs (Supplementary
Material, Table S3).
Our sequential conditional model included three SNPs from
region 1 (rs4430796, rs74056969, rs1016990) and two SNPs
from region 2 (rs4794758, rs3094509). A haplotype analysis
of these five SNPs revealed that the most significant haplotype
associated with risk carried the protective allele at rs4430796,
rs74056969 and rs4794758 (P ¼ 2.78 × 1028) (Supplemen-
tary Material, Table S4). When the combined risk of all five
SNPs was examined, a dose–response was observed with
increasing number of risk variants (Ptrend¼ 1.94 × 10226,
Table 1. SNPs in the HNF1B region that were associated with prostate cancer at genome-wide significance levels (P , 5.0 × 1028)
Base pair position
Minor allele
Other allele
Minor allele
frequency: cases
Minor allele
frequency: controls
No. of
No. of
OR (95% CI)a
Heterogeneity P
0.81 (0.77–0.84)
1.62 × 10224
0.81 (0.78–0.85)
2.54 × 10223
1.23 (1.18–1.28)
9.35 × 10223
0.84 (0.80–0.87)
1.22 × 10216
0.84 (0.80–0.87)
1.57 × 10216
0.84 (0.80–0.88)
1.39 × 10215
1.19 (1.14–1.24)
4.45 × 10215
0.86 (0.82–0.90)
4.95 × 10210
0.89 (0.85–0.92)
1.21 × 10208
0.86 (0.82–0.91)
3.54 × 10208
aOdds ratio per minor allele.
3324Human Molecular Genetics, 2011, Vol. 20, No. 16
Page 4
Table 3). Men with eight or more risk alleles had a 1.88-fold
increased risk of prostate cancer compared with men with
zeroto tworisk alleles
P ¼ 4.29 × 1029). In comparison, when only the most signifi-
cant SNP from region 1 (rs4430796) and region 2 (rs4794758)
were examined, men with four or more risk alleles had a
1.69-fold risk compared with men with no risk alleles (95%
10225. The P-value for the number of risk alleles for the
three other SNPs from the sequential model modeled as a con-
tinuous variable was 1.63 × 1025. The variance explained by
Ptrend¼ 1.80 ×
the five SNPs was 0.9% compared with 0.7% for the two best
SNPs from region 1 and region 2.
Finally, we conducted stratified analyses by family history
of prostate cancer and did observe a qualitative interaction
for rs2107131 (Pinteraction¼ 4.29 × 1025) that remained stat-
istically significant after adjustment for multiple testing
(Padj¼ 0.003) (Supplementary Material, Table S5). Among
men with a family history of prostate cancer, the T allele at
rs2107131 was associated with a reduced risk of prostate
cancer (OR ¼ 0.85; 95% CI: 0.74–0.97), whereas among
men without a family history, it was associated with an
Figure 1. Prostate cancer risk associated with SNPs in the HNF1B region: (A) results from the unconditional analysis; (B) results after conditioning on
rs4430796. The diamond indicates the most statistically significant SNP in the region.
Human Molecular Genetics, 2011, Vol. 20, No. 16 3325
Page 5
increased risk of prostate cancer (OR ¼ 1.13; 95% CI: 1.08–
1.19). Stratified analyses were also performed for prostate
cancer aggressiveness (Gleason , 7 and Stage A/B versus
Gleason ≥ 7 or Stage C/D); however, there were no significant
differences beyond what would be expected by chance (Sup-
plementary Material, Table S6). We did not observe any sig-
nificant heterogeneity between studies beyond what would
be expected by chance (Supplementary Material, Table S1),
except for rs1058166 (Pheterogeneity¼ 0.0002).
Our fine mapping study of a region on 17q12 associated with
prostate cancer confirmed the previously established signals
(6,7,10) and found evidence that additional variants contribute
to the risk of prostate cancer. Although rs4430796 was the
most significant SNP associated with risk, rs7405696 was
also significant at a genome-wide significance level and
explained part of the risk associated with the first HNF1B
locus, suggesting a more complex genetic architecture for
common variants in this region. Since this study used SNP
markers, further work is needed to investigate the biological
basis of the association with common variants in 17q12,
which may regulate HNF1B, or perhaps another gene in pros-
tate cancer. It is plausible that multiple variants are directly
associated with prostate cancer susceptibility.
In the second HNF1B locus, we found that rs4794758 was
more strongly associated with risk than the previously ident-
ified variant, rs11649743. When both variants were included
in the same model, rs4794758 explained all of the risk associ-
ated with rs11649743, indicating that this variant more aptly
captured the risk attributable to this locus. Although the first
and second HNF1B loci are separated by recombination
hotspot (10), the two loci are not completely independent
and the risk associated with rs4794758 was attenuated after
conditioning on the most significant SNP in the first locus,
In our study, the best model for prostate cancer risk in the
HNF1B region included five SNPs (rs4430796, rs7405696,
rs4794758, rs1016990 and rs3094509). Although three of
these SNPs reached genome-wide significance in uncondi-
tional models, rs1016990 was only nominally associated
with risk (P ¼ 0.0002) and rs3094509 was not associated
with risk (P ¼ 0.80) in unconditional models. Together,
Table 2. Final prostate cancer risk models for HNF1B SNPs from the sequential conditional, stepwise regression and lasso analysesa
Sequential conditional model
Forward stepwise regression
Lasso model
Two SNP model
Base pair position
Minor allele
Other allele
ORb(95% CI)
ORb(95% CI)
ORb(95% CI)
ORb(95% CI)
0.87 (0.80–0.93)
0.86 (0.80–0.92)
0.93 (0.83–1.04)
0.82 (0.79–0.86)
0.95 (0.86–1.04)
1.11 (1.03–1.18)
1.09 (1.02–1.17)
1.10 (1.02–1.18)
0.88 (0.84–0.93)
0.89 (0.85–0.94)
0.94 (0.87–1.02)
0.91 (0.86–0.95)
0.94 (0.87–1.03)
1.07 (1.01–1.12)
1.07 (1.02–1.13)
1.06 (1.01–1.10)
aFor comparison, all analyses were restricted to subjects with complete genotyping for these SNPs, so that the total number of subjects included in each model was the same.
bOdds ratio per minor allele.
Table 3. Risk of prostate cancer associated with the count of HNF1B risk alle-
No. of risk
No. of
No. of
1.10 (0.93–1.31)
1.29 (1.10–1.52)
1.33 (1.13–1.55)
1.66 (1.42–1.95)
1.71 (1.44–2.03)
1.88 (1.52–2.33)
aIncludes the five SNPs identified in the sequential conditional analysis
(rs4430796, rs7405696, rs4794758, rs1016990 and rs3094509).
3326 Human Molecular Genetics, 2011, Vol. 20, No. 16
Page 6
these five variants provided a better model for the data than
other combinations, suggesting that these SNPs together
capture more of the risk associated with this region. Further-
more, haplotype analysis revealed that risk was not explained
by a single haplotype, suggesting a role for multiple variants
or combinations of variants. Imputation using data from the
1000 Genomes Project yielded similar results; however,
additional variants discovered in future next generation
sequencing may also contribute to risk.
Interestingly, we observed a significant interaction between
a SNP located in the second HNF1B locus, rs2107131, and
family history of prostate cancer. Family history was only cap-
tured at baseline for participants and could have changed over
time, and the number of subjects with a positive family history
of prostate cancer was limited (N ¼ 2182 men), so this finding
should be interpreted with caution. However, it is possible that
the interaction reflects haplotype segregation with uncommon
disease-causing alleles associated with familial cases and
common disease-causing alleles associated with sporadic
The biological mechanism by which 17q12 variants may
alter the regulation or splicing of a plausible candidate gene,
such as HNF1B, is not clear. Down-regulation of HNF1B
expression has been associated with renal cell cancer pro-
gression (24), and differential expression of HNF1B has
beenassociatedwith prostate
HNF1B is a transcription factor that encodes three isoforms
in humans. Isoforms HNF1B(A) and HNF1B(B) appear to be
transcriptional activators, whereas isoform HNF1B(C) is a
transcriptional repressor (25). Differences in isoform-specific
HNF1B expression have also been observed between normal
and malignant prostate tissue, with prostate tumors displaying
greater isoform HNF1B(B) expression but less isoform
HNF1B(C) expression than normal prostate tissue (26). It is
possible that variants in HNF1B contribute to the altered the
expression of HNF1B isoforms in prostate cancer. It is also
plausible that variants in this region of 17q12 could also influ-
ence the regulation or expression of other genes at a distance.
In conclusion, this large-scale fine mapping study revealed
that the association between variants in the 17q12 region
and prostate cancer risk is more complex than earlier studies
have indicated. Additional sequencing and functional studies
are needed to pinpoint the variants in this region that are
directly associated with prostate cancer risk and the biological
mechanisms involved.
cancer recurrence(23).
Study subjects
As described previously (11), prostate cancer cases and con-
trols of European ancestry were drawn from 10 studies in
the USA and Europe: Prostate, Lung, Colorectal, and
Ovarian (PLCO) Cancer Screening Trial (972 cases/927 con-
trols); Alpha-Tocopherol, Beta-Carotene Cancer Prevention
Study (ATBC) (906 cases/868 controls); American Cancer
Society Cancer Prevention Study II (CPSII) (1643 cases/
1640 controls);Health Professionals
(HPFS) (595 cases/589 controls); CeRePP French Prostate
Follow-up Study
(998cases/952 controls);
Multiethnic Cohort Study (MEC) (676 cases/682 controls);
European Prospective Investigation into Cancer and Nutrition
(EPIC) (682 cases/990 controls); Cohort of Norway (CONOR)
(606 cases/662 controls); Cancer of the Prostate in Sweden
(CAPS) (2213 cases/1362 controls); and a hospital-based
case–control from the Johns Hopkins Hospital (JHH) (990
cases/451 controls). A total of 10 272 prostate cancer cases
and 9123 controls were available for this study. Aggressive
prostate cancer was defined at Gleason score ≥7 or stage C/
D. Among the cases with information on disease aggressive-
ness, 4824 cases were defined as aggressive and 4337 were
non-aggressive (Gleason score ,7 and stage A/B). Family
history of prostate cancer was obtained by self-report from
the participants and available for 8 of the 10 studies. A posi-
tive family history of prostate cancer was defined as a first
degree relative with prostate cancer. Each study obtained an
informed consent from study participants and approval from
their respective institutional review boards for this study.
SNP selection
A total of 88 SNPs within a 249 071 bp region encompassing
HNF1B were selected for fine mapping. The SNPs were
chosen based on the 0.2 cM HapMap recombination region
flanking the most significant SNP (rs4430796) in the HNF1B
locus from the second stage of the CGEMS GWAS (7). The
entire region was tagged to capture SNPs with a minor allele
frequency ≥5% at a D′. 0.6 based on the HapMap CEU
population (Build 26). All three SNPs with a P , 1023from
the second stage of CGEMS in this region (i.e. rs4430796,
rs7501939 and rs11649743) were selected as obligated SNPs
to be included. The final tag SNPs were selected if they
were found to be correlated with an r2of ≥0.8 in the
HapMap CEU, YRI or JPT + CHB populations with the obli-
gated SNPs. The tag SNP selection was performed using
theGLUsoftware package
glu-genetics/). The chosen SNPs were primarily located in
first (chromosome 17: 33.161–33.205 Mb, NCBI Build 36)
and second (chromosome 17: 33.116–33.161 Mb, NCBI
Build 36) HNF1B regions identified to be associated with
prostate cancer risk; however, other SNPs outside these
regions were also included.
All SNPs for this study were genotyped on a custom Illumina
iSelect assay panel as part of the third stage of CGEMS as
described previously (11). In brief, a total of 6652 SNPs,
including 1400 SNPs to monitor population stratification,
were attempted in 22 057 samples, including quality control
duplicates. Duplicate samples yielded a 99.97% concordance
rate. Subjects with ,90% completion (n ¼ 1350), missing
covariate data or sparse group (n ¼ 104), likely an intra-study
duplicate based on genotype concordance ≥99% (n ¼ 18), or
non-European ancestry defined by ,0.80 European admixture
as estimated using STRUCTURE (27) (n ¼ 372) were
excluded, leaving 10 272 cases and 9123 controls for analysis.
SNPs with ,80% completion within a study were removed
from analysis for that study. SNPs that failed to provide gen-
otypes for more than six studies (n ¼ 1), had a minor allele
Human Molecular Genetics, 2011, Vol. 20, No. 163327
Page 7
count ≤10 (n ¼ 7) or had genotypes that were inconsistent
with Hardy–Weinberg proportions
(P , 0.001) (n ¼ 1) were excluded from analysis, leaving 79
SNPs for analysis.
To explore the possibility that other variants in the region
could be related to risk, we imputed the 249 071 bp region
encompassing our genotyped SNPs using IMPUTE2 and the
1000 Genomes Project data (June 2010 release). A total of
653 SNPs were imputed from this region, but only SNPs
with a quality score (i.e. info) .0.40 were considered for
analysis (N ¼ 353). All imputed SNPs were analyzed using
SNPTEST v.2.2.0 accounting for imputation uncertainty.
Statistical analysis
Principal components analysis was conducted using EIGEN-
STRAT (28) with 1399 SNPs that were genotyped, passed
quality control criteria and selected for population stratifica-
tion; these SNPs were chosen because they had minimal cor-
relation (r2, 0.004) (29). The Wilcoxon rank test was used
to test the association between the top five eigenvectors and
case–control status. Four eigenvectors displayed a significant
or borderline significant association with prostate cancer
(P , 0.08) and were included in the analysis. The association
between each SNP and prostate cancer risk was estimated
using logistic regression adjusting for age (,50, 50–59,
60–69, 70–79, ≥80), study (including country for EPIC)
and significant principal components. Stratified analyses
were conducted to examine differences by disease aggressive-
ness, family history and study. Heterogeneity between aggres-
sive and non-aggressive disease was assessed in a case-only
analysis using logistic regression. Heterogeneity between
studies and risk modification by family history was assessed
using a likelihood ratio test comparing the model with and
without the cross-product term(s). P-values for interactions
tests were adjusted for the false discovery rate (30).
To explore the interdependence of the associations
observed, three separate approaches were taken: (i) sequential
conditional analyses, (ii) stepwise regression, and (iii) lasso
(31). Sequential conditional analyses were conducted by
including the most significant SNP in the unconditional logis-
tic regression model followed by sequential inclusion of the
most significant SNP in each conditional model and examining
the association with each of the remaining SNPs indepen-
dently. Forward stepwise regression was performed using the
SNPs that reached a nominal significance level in the uncondi-
tional model. SNPs were sequentially included in the model
based on a minimal P , 0.05. Lasso was conducted in R
using all SNPs without missing data and an unweighted
penalty function.
Linkage disequilibrium measures (D′and r2) were esti-
mated in the controls using Haploview. Haplotypes were
estimated using an expectation-maximization algorithm and
analyzed using the generalized linear regression model
implemented in HaploStats (32). Unless otherwise indicated,
all analyses were conducted using PLINK or STATA.
Supplementary Material is available at HMG online.
This study was supported by the Intramural Research Program
of the Division of Cancer Epidemiology and Genetics,
National Cancer Institute, National Institutes of Health
(NIH). The content of this publication does not necessarily
reflect the views or policies of the Department of Health and
Human Services nor does mention of trade names, commercial
products or organization indicate endorsement by the US Gov-
ernment. The authors thank Drs Christine Berg and Philip
Prorok, Division of Cancer Prevention, NCI, the screening
center investigators and staff of the PLCO Cancer Screening
Trial, Mr Thomas Riley and staff at Information Management
Services, Inc., and Ms Barbara O'Brien and staff at Westat,
Inc. for their contributions to the PLCO. Finally, we are grate-
ful to the study participants for donating their time and making
this study possible.
Conflict of Interest statement. None declared.
This study was supported by the Intramural Research Program
of the Division of Cancer Epidemiology and Genetics,
National Cancer Institute, National Institutes of Health and
in part by National Cancer Institute, National Institutes of
Health(CA129684to J.X.)
and contractnumber
1. Lichtenstein, P., Holm, N.V., Verkasalo, P.K., Iliadou, A., Kaprio, J.,
Koskenvuo, M., Pukkala, E., Skytthe, A. and Hemminki, K. (2000)
Environmental and heritable factors in the causation of cancer—analyses
of cohorts of twins from Sweden, Denmark, and Finland. N. Engl. J. Med.,
343, 78–85.
2. Amundadottir, L.T., Sulem, P., Gudmundsson, J., Helgason, A., Baker, A.,
Agnarsson, B.A., Sigurdsson, A., Benediktsdottir, K.R., Cazier, J.B.,
Sainz, J. et al. (2006) A common variant associated with prostate cancer
in European and African populations. Nat. Genet., 38, 652–658.
3. Yeager, M., Orr, N., Hayes, R.B., Jacobs, K.B., Kraft, P., Wacholder, S.,
Minichiello, M.J., Fearnhead, P., Yu, K., Chatterjee, N. et al. (2007)
Genome-wide association study of prostate cancer identifies a second risk
locus at 8q24. Nat. Genet., 39, 645–649.
4. Gudmundsson, J., Sulem, P., Manolescu, A., Amundadottir, L.T.,
Gudbjartsson, D., Helgason, A., Rafnar, T., Bergthorsson, J.T.,
Agnarsson, B.A., Baker, A. et al. (2007) Genome-wide association study
identifies a second prostate cancer susceptibility variant at 8q24. Nat.
Genet., 39, 631–637.
5. Haiman, C.A., Patterson, N., Freedman, M.L., Myers, S.R., Pike, M.C.,
Waliszewska, A., Neubauer, J., Tandon, A., Schirmer, C., McDonald, G.J.
et al. (2007) Multiple regions within 8q24 independently affect risk for
prostate cancer. Nat. Genet., 39, 638–644.
6. Gudmundsson, J., Sulem, P., Steinthorsdottir, V., Bergthorsson, J.T.,
Thorleifsson, G., Manolescu, A., Rafnar, T., Gudbjartsson, D., Agnarsson,
B.A., Baker, A. et al. (2007) Two variants on chromosome 17 confer
prostate cancer risk, and the one in TCF2 protects against type 2 diabetes.
Nat. Genet., 39, 977–983.
7. Thomas, G., Jacobs, K.B., Yeager, M., Kraft, P., Wacholder, S., Orr, N.,
Yu, K., Chatterjee, N., Welch, R., Hutchinson, A. et al. (2008) Multiple
3328 Human Molecular Genetics, 2011, Vol. 20, No. 16
Page 8
loci identified in a genome-wide association study of prostate cancer. Nat.
Genet., 40, 310–315.
8. Eeles, R.A., Kote-Jarai, Z., Giles, G.G., Olama, A.A., Guy, M.,
Jugurnauth, S.K., Mulholland, S., Leongamornlert, D.A., Edwards, S.M.,
Morrison, J. et al. (2008) Multiple newly identified loci associated with
prostate cancer susceptibility. Nat. Genet., 40, 316–321.
9. Gudmundsson, J., Sulem, P., Rafnar, T., Bergthorsson, J.T., Manolescu,
A., Gudbjartsson, D., Agnarsson, B.A., Sigurdsson, A., Benediktsdottir,
K.R., Blondal, T. et al. (2008) Common sequence variants on 2p15 and
Xp11.22 confer susceptibility to prostate cancer. Nat. Genet., 40,
10. Sun, J., Zheng, S.L., Wiklund, F., Isaacs, S.D., Purcell, L.D., Gao, Z., Hsu,
F.C., Kim, S.T., Liu, W., Zhu, Y. et al. (2008) Evidence for two
independent prostate cancer risk-associated loci in the HNF1B gene at
17q12. Nat. Genet., 40, 1153–1155.
11. Yeager, M., Chatterjee, N., Ciampa, J., Jacobs, K.B., Gonzalez-Bosquet,
J., Hayes, R.B., Kraft, P., Wacholder, S., Orr, N., Berndt, S. et al. (2009)
Identification of a new prostate cancer susceptibility locus on
chromosome 8q24. Nat. Genet., 41, 1055–1057.
12. Al Olama, A.A., Kote-Jarai, Z., Giles, G.G., Guy, M., Morrison, J., Severi,
G., Leongamornlert, D.A., Tymrakiewicz, M., Jhavar, S., Saunders, E.
et al. (2009) Multiple loci on 8q24 associated with prostate cancer
susceptibility. Nat. Genet., 41, 1058–1060.
13. Eeles, R.A., Kote-Jarai, Z., Al Olama, A.A., Giles, G.G., Guy, M., Severi,
G., Muir, K., Hopper, J.L., Henderson, B.E., Haiman, C.A. et al. (2009)
Identification of seven new prostate cancer susceptibility loci through a
genome-wide association study. Nat. Genet., 41, 1116–1121.
14. Gudmundsson, J., Sulem, P., Gudbjartsson, D.F., Blondal, T., Gylfason,
A., Agnarsson, B.A., Benediktsdottir, K.R., Magnusdottir, D.N.,
Orlygsdottir, G., Jakobsdottir, M. et al. (2009) Genome-wide association
and replication studies identify four variants associated with prostate
cancer susceptibility. Nat. Genet., 41, 1122–1126.
15. Takata, R., Akamatsu, S., Kubo, M., Takahashi, A., Hosono, N.,
Kawaguchi, T., Tsunoda, T., Inazawa, J., Kamatani, N., Ogawa, O. et al.
(2010) Genome-wide association study identifies five new susceptibility
loci for prostate cancer in the Japanese population. Nat. Genet., 42,
16. Zeggini, E., Scott, L.J., Saxena, R., Voight, B.F., Marchini, J.L., Hu, T.,
de Bakker, P.I., Abecasis, G.R., Almgren, P., Andersen, G. et al. (2008)
Meta-analysis of genome-wide association data and large-scale replication
identifies additional susceptibility loci for type 2 diabetes. Nat. Genet., 40,
17. Kasper, J.S. and Giovannucci, E. (2006) A meta-analysis of diabetes
mellitus and the risk of prostate cancer. Cancer Epidemiol. Biomarkers
Prev., 15, 2056–2062.
18. Stevens, V.L., Ahn, J., Sun, J., Jacobs, E.J., Moore, S.C., Patel, A.V.,
Berndt, S.I., Albanes, D. and Hayes, R.B. (2010) HNF1B and JAZF1
genes, diabetes, and prostate cancer risk. Prostate, 70, 601–607.
19. Kato, N. and Motoyama, T. (2009) Hepatocyte nuclear
factor-1beta(HNF-1beta) in human urogenital organs: its expression and
role in embryogenesis and tumorigenesis. Histol. Histopathol., 24,
20. Maestro, M.A., Cardalda, C., Boj, S.F., Luco, R.F., Servitja, J.M. and
Ferrer, J. (2007) Distinct roles of HNF1beta, HNF1alpha, and HNF4alpha
in regulating pancreas development, beta-cell function and growth.
Endocr. Dev., 12, 33–45.
21. Bellanne-Chantelot, C., Chauveau, D., Gautier, J.F., Dubois-Laforgue, D.,
Clauin, S., Beaufils, S., Wilhelm, J.M., Boitard, C., Noel, L.H., Velho, G.
and Timsit, J. (2004) Clinical spectrum associated with hepatocyte nuclear
factor-1beta mutations. Ann. Intern. Med., 140, 510–517.
22. Edghill, E.L., Bingham, C., Ellard, S. and Hattersley, A.T. (2006)
Mutations in hepatocyte nuclear factor-1beta and their related phenotypes.
J. Med. Genet., 43, 84–90.
23. Glinsky, G.V., Glinskii, A.B., Stephenson, A.J., Hoffman, R.M. and
Gerald, W.L. (2004) Gene expression profiling predicts clinical outcome
of prostate cancer. J. Clin. Invest., 113, 913–923.
24. Buchner, A., Castro, M., Hennig, A., Popp, T., Assmann, G., Stief, C.G.
and Zimmermann, W. (2010) Downregulation of HNF-1B in renal cell
carcinoma is associated with tumor progression and poor prognosis.
Urology, 76, 507–511.
25. Bach, I. and Yaniv, M. (1993) More potent transcriptional activators or a
transdominant inhibitor of the HNF1 homeoprotein family are generated
by alternative RNA processing. EMBO J., 12, 4229–4242.
26. Harries, L.W., Perry, J.R., McCullagh, P. and Crundwell, M. (2010)
Alterations in LMTK2, MSMB and HNF1B gene expression are
associated with the development of prostate cancer. BMC Cancer,
10, 315.
27. Pritchard, J.K., Stephens, M. and Donnelly, P. (2000) Inference of
population structure using multilocus genotype data. Genetics, 155,
28. Price, A.L., Patterson, N.J., Plenge, R.M., Weinblatt, M.E., Shadick, N.A.
and Reich, D. (2006) Principal components analysis corrects for
stratification in genome-wide association studies. Nat. Genet., 38,
29. Yu, K., Wang, Z., Li, Q., Wacholder, S., Hunter, D.J., Hoover, R.N.,
Chanock, S. and Thomas, G. (2008) Population substructure and control
selection in genome-wide association studies. PLoS ONE, 3, e2551.
30. Benjamini, Y. and Hochberg, Y. (1995) Controlling the false discovery
rate: a practical and powerful approach to multiple testing. J. R. Statist.
Soc. B, 57, 289–300.
31. Tibshirani, R. (1996) Regression shrinkage and selection via lasso. J. R.
Stat. Soc. B, 58, 267–288.
32. Schaid, D.J., Rowland, C.M., Tines, D.E., Jacobson, R.M. and Poland,
G.A. (2002) Score tests for association between traits and haplotypes
when linkage phase is ambiguous. Am. J. Hum. Genet., 70, 425–434.
Human Molecular Genetics, 2011, Vol. 20, No. 163329


text:
Estelle @EstelleDarlings
Tweet: "#tbt of life lol ok so I started as a rapper. This is when I was an Independent mistake artist on… http://instagr.am/p/WSF1pKrXpD/"
Check out this great pic of a young Estelle with Talib Kweli and Mos Def when she was just a rapper on her own indie label in London. (Photo: D Dipasupil/Getty Images for BET)
Instagram Photos of the Week: Dej Loaf Shows Her Sexy Side03/27/2015
Tweets of the Week: 50 Cent Praises Patti LaBelle's Dance03/27/2015
Class Act: Musicians in the Classroom03/27/2015
Eazy-E's Influence on Hip Hop03/26/2015
The Rundown: Earl Sweatshirt, I Don't Like S**t03/25/2015
Music Stars We Lost to Overdoses03/25/2015
Things We Learned From Action Bronson on The Breakfast Club03/24/2015
Hit 'Em Up: Music Stars Provoked to Punch, Allegedly03/24/2015
Music Videos Based on Movies03/24/2015
The Rundown: Kendrick Lamar, To Pimp a Butterfly03/23/2015
The Rundown: Action Bronson, Mr. Wonderful03/23/2015
Bail Amounts That'll Knock You Out03/23/2015
Stage Struggles: Strange Fan Encounters at Shows03/23/2015
Best Instagram Photos From SXSW03/22/2015
Stop the Presses: When Music Stars Clash With the Media03/22/2015
John Legend's One Night for ONE DROP03/21/2015
Risk-Taking Albums With Kendrick Lamar's Restless Sound03/21/2015
Out and About: SXSW Music Edition03/21/2015
Instagram Photos of the Week: Beyoncé Styles On The 'Gram03/20/2015
Breaking Tradition: Albums That Didn't Drop on a Tuesday03/20/2015
Tweets of the Week: Drake Blasts New Concert Doc, Homecoming03/20/2015
Wildest Petitions Aimed at Music Stars03/19/2015
When Artists Fight Over a Group Name03/19/2015
Music Stars and Lawsuits03/19/2015
The Evolution of Erykah Badu03/18/2015
10 Classic R&B Mixtapes You Definitely Need03/18/2015
Nicki Minaj Comes in First: A Look at Her Milestones03/18/2015
Prison Song: MCs Who've Released Albums From Jail03/18/2015
In My Lifetime: Music Biopics03/18/2015
Twitter Reacts To Young Thug's Carter 603/18/2015
20 Blackest Lines From Kendrick Lamar's To Pimp a Butterfly03/17/2015
10 Things You Should Know About Rapsody03/17/2015
Music Stars React to Kendrick Lamar To Pimp a Butterfly03/16/2015
7 Reasons the 'Blurred Lines' Verdict Should Spook03/13/2015
Tweets of the Week: The O.M.G. Girlz Call It Quits03/13/2015
Instagram Photos of the Week: Drake's Dreams in Dubai03/13/2015

subset: commoncrawl


text:
Select Language
My Bag0
My Favorites0
Estimated Delivery : Apr 04 - Apr 06
Estimated Delivery :
BB Dakota is an eclectic shake up of the modern girl. We believe that fashion is about self-expression and taking risks, but more importantly, it?s about having fun. We design for the rule-breakers, the dreamers? BB Dakota supports the moment.
If an item you would like to purchase is out of stock, you can place a Special Order Inquiry. REVOLVE will then check the availability and obtain an approximate arrival date for the item, if available. Typically, it takes up to 3-4 weeks to verify this information.
Placing a Special Order Inquiry is absolutely free and there is no obligation to follow through with a purchase.
Sort by body type:
It was a little shorter than I had expected it to be in my mind. This is all on me because it does look super short in the photo. I was planning to wear with a belt like the photo, but it's too short. Love it though! There is a removable slip layer to keep it from being see-through, so the quality is great for the price!


text:
Shop now and get Free Value Shipping on most orders over $49 to the contiguous 48 states, DC and to all U.S. Military APO/FPO/DPO addresses.
We want to ensure that making a return is as easy and hassle-free as possible! If for any reason you decide that your purchase just isn't for you, simply return the item within 30 days of receipt and we'll cover the cost of return shipping. Learn more about our Return Policy.
With the Outdoor Edge Cutlery SwingBabe Knife, simply push the lock button and the SwingBabe changes from a drop-point skinner to the ultimate gutting blade to open game like a zipper. This innovative Outdoor Edge Cutlery SwingBabe Folding Knife has a blade that cuts under the skin eliminating the chance of cutting hair or internal organs. The Outdoor Edge Cutlery SwingBabe Knife for Her comes with a hot-pink Kraton handle, so there's no mistaking who's knife this is.
Connect with OpticsPlanet
Become our fan, follow us, & subscribe for the latest updates and deals
Subscribe to our email list
Sign up and receive $5 Off and get exclusive access to promotions, sales
events, pre-order sales & more!
Contact Information
Toll-Free: 800-504-5897
Intl.: 847-513-6201
Fax: 847-919-3003
Email: firstname.lastname@example.com
Hours of Operation
7AM-8PM CST Mon-Fri9AM-5PM CST Sat-Sun
Our Guarantees


text:
Good Words for Halifax Harbor Marina, AICW Statute Mile 831
Halifax Harbor Marina is in Daytona Beach on the western shores of the ICW/Halifax River, south of unlighted daybeacon #39A.
I suggest you stay at the Halifax Harbor Marina, I lived there for two years and the people and services were great, and you could not beat the rates, for liveaboards, I would still be there if work did not keep me where I am now.
Sean Hickey
We just stayed there last week & it is a great place.
Cheryl Jones
Click Here To View the Cruisers' Net's Eastern Florida Marina Directory Listing For Halifax Harbor Marina
Click Here To Open A Chart View Window, Zoomed To the Location of Halifax Harbor Marina


text:
Narrow your search
RSA Conference follows other associations in writing new dress codes for its exhibitors. And that means no more tube tops, minidresses or bodysuits for women -- or men -- staffing their booths.
Google tries hard to maintain a sense of humor through surprises tucked throughout its Web properties. Here are all the best Easter eggs you need to check out.
We found 27 awesome Google Easter eggs so you don't have to.
Launch Center Pro may have removed Foursquare from its supported app list, but there are ways to still get it to work; it just takes a little extra effort.
We take a close-up look at the Vector smartwatch, which boasts 30-day battery life, smart functions and a cool metal design.
The latest Motorola Moto E proves you can make an affordable phone that's fun, too.
Astronaut Terry Virts has been on the ISS since December, taking video. Luckily for us, he likes to share.
The first-ever sedan from the Scion brand is technically a Mazda.
Cadillac defies conventional wisdom for its new flagship luxury sedan, offering not eight, but six cylinders under the hood.
Pricing not available
With the introduction of the new Macbook, the first notebook that charges via a Type-C USB (or USB-C) port, the new USB port standard is going to take off.


text:
Room for one more?
OK DH thinks I have too many and certainly do not need any
more plumies. I say there is always room for more.
What do you think?
Actually I just needed an excuse to practice posting pics
here. I'm still pretty clumbsy and slow with this photo sharing stuff.
Dave is right...I am sso jealous. I also agree that they are still small but even at that, you live in SOCAl Zone 10! Why not have more? Until it gets to the point where you cannot walk around to enjoy them anymore, I say BUY MORE!!
Now I have a problem! At last count I am at 55 and thanks to Sunseeker, I just planted about 8 seeds. I also have a 'Scentsational' cutting on it's way, and I am buying 3 or 4 more. That will put me around 60, and I live in Zone 6 NY state! They are going to have to be crammed in my basement, and spare bedrooms!


text:
no clip install stainless steel kitchen sink
To make this brief, I am trying to replace my kitchen sink. Laminate coutertops, hole is right size but there is no overlap of countertop to attach sink clips.
There is 1/2" thick base cabinet wood walls on either side so I was thinking of routing or chiseling little notches to fit the sink clips and give them something to bite into.
Then I thought an easier way would be to go ahead and silicone the sink in place and then put a 5 gallon bucket of water in each bowl to provide weight and let it sit for 12-24 hours.
Any ideas??
I used the 'glue it with caulk' technique about 15 years ago and have had no problems. Removing the sink, however, would probably end up with its edges being bent up beyond reuse.
When trying the notch technique, I found it difficult to impossible to work in the narrow space available to square up the clips.


text:
Canstar Community News - ONLINE EDITION
Miles Macdonell goes all out for newcomers
Miles Macdonell Collegiate has been going the extra step to welcome new students into the school the past two years.
For 2014-15, the East Kildonan school is kicking it up a notch.
For the third installment of the R U BUCK ENUF? event on Sept. 11, the school is inviting The Amazing Race Canada participants Nicole and Cormac Foster, the latter a 2012 grad of the school, to help out. As well, Energy 106 hosts Jordan Knight and Chris Reid will be on-site broadcasting.
Organizing teacher Colin McDonald brought the idea from his former school, John Henderson Junior High School, where incoming students participated in an "intramural all-nighter."
McDonald modified the idea to make it a cross between the Survivor and The Amazing Race reality television game shows in order to get pupils to explore their new surroundings.
"When I made the jump to high school, I said 'Why not add something like this for the Grade 10s, because it's very intimidating,'" he said. "I came up with the idea of doing something fun where we get them exposed to the whole school.
"It's really grown; it's a really positive thing this year."
McDonald explained the teams will include about 10 to 12 members apiece, and he hopes to have about half the incoming Grade 10s participate, as it is not a mandatory event.
Registration is $10 per student and includes a T-shirt, pizza dinner, and other goodies.
McDonald said he is hoping to spread the concept, as he invited a staff member and student council member from some other River East Transcona School Division schools to participate.
Organizer Andrew Hector, who will be entering Grade 12 this fall, recalled his participation in the inaugural event. He said since there are several feeder schools that funnel students to Miles Macdonell, he appreciated the opportunity to meet those he didn't know previously.
"There's a physical and a mental part to it, so you have to interact as one unit to get to your next task," Hector said.
Fellow organizer Brae Brandt, also entering Grade 12, said she was still learning about the school when she helped out with last year's event, discovering the school's foods room. She added the difference in the community immediately afterward is tangible.
"You notice the buzz the day after," Brandt said. "In the tunnel, everyone's talking, everyone's excited, and it's a whole different atmosphere."
The Fosters, meanwhile, are thrilled to be able to use their newfound celebrity in positive ways, including passing on a message of never giving up. Though Cormac didn't get to participate in the event in previous years, he understands its importance.
"You start to develop a connection with the school and be proud to be a Buckeye," said Cormac, who attended the school for Grades 11 and 12.
Twitter: @HeraldWPG
Fact Check
Have you found an error, or know of something we've missed in one of our stories?
Please use the form below and let us know.
Having problems with the form?Contact Us Directly
More The Herald
You can comment on most stories on winnipegfreepress.com. You can also agree or disagree with other comments. All you need to do is be a Winnipeg Free Press print or e-edition subscriber to join the conversation and give your feedback.
You can comment on most stories on winnipegfreepress.com. You can also agree or disagree with other comments. All you need to do is be a Winnipeg Free Press print or e-edition subscriber to join the conversation and give your feedback.
Have Your Say
New to commenting? Check out our Frequently Asked Questions.
The Winnipeg Free Press does not necessarily endorse any of the views posted. By submitting your comment, you agree to our Terms and Conditions. These terms were revised effective April 16, 2010.
- Back to Top
- Return to The Herald
More The Herald
(1 of 9 articles for this week)
Help locals make a frackin' movie03/30/2015 2:25 PM 0
This Just In
@CanstarHeadline: Headingley residents nominated for YMCA-YWCA Women of Distinction awards. winnipegfreepress.com/our-communitie… #cbn 12:13 pm on Mar 30
Ads by Google


text:
Valkyrie Rising
From the critics
Community Activity
CommentAdd a Comment
This book was interesting because of the mythology and very girly power, which kept me reading. It was too drawn out, however, and the ending left me unsatisfied.
Couldn't get past the first 70 pages or so. *Yawn*
it wasnt too bad of a book. very interesting. it has you wanting a sequel by the way it ends
AgeAdd Age Suitability
There are no ages for this title yet.
SummaryAdd a Summary
There are no summaries for this title yet.
NoticesAdd a Notice
There are no notices for this title yet.
QuotesAdd a Quote
There are no quotes for this title yet.


text:
motion + interaction: little rock media production & development consulting.
We're fueled by the power of visuals and communication. Canticle crafts digital content & strategies for NGOs, not-for-profits, and corporate clients. Increase your marketing return on investment through creating a story with Canticle.
Animation, editing, motion graphics, TV spots. We've filmed in 22 countries, spent 20-hour jaunts in After Effects, ridden motorbikes through Uganda, taken cameras to the center of Kibera & driven 3000km through India.
We like spartan code & pixel-perfect visuals, everything from a database backend to a mobile app to a bootstrapped website. Python, C#, C++, PHP, JavaScript, HTML5, and CSS are all languages we speak.
Liberian ebola units in hazmat suits. Beaches in Costa Rica. On a ship in the British Channel. In a middle-America cubicle with animated financial graphs. For an updated web portfolio, shoot us an email (we've got an awesome spread of smooth-scrolling media-infused parallax sites).
We're located in Little Rock, but the first commercial airline flight was in 1914. One hundred and one years later, it's 2015. Give us a shout. We'll fly to you.
Normally installed into the corner of (any) coffeeshop. Nestled snugly in Little Rock. Airport groupie & spreadsheet addict. Enjoys eating bad food, from India to Liberia to Costa Rica. Can actually swap out a transmission.After Effects Premiere Pro HTML5 CSS3 PBR Coffee Nikon Soviet Russia BMW
Nerds aren't always introverts. Currently located in the middle of Dallas-Fort Worth sprawl. Open source proponent. Backend developer & technical lead, probably why he cannot sing. Visit him at ashtonkinslow.com..NET PHP C# JavaScript White Russians Paintball Mobile Dev C++ SQL
Probably the nicest guy you've met. Graphic designer based just outside of Atlanta. Rockstar bassist who's toured the United States melting the faces off thousands of alt rock fans. Take a look at his portfolio: johnpackerdesign.com.Photoshop Illustrator InDesign Electric Bass Eagles Art Comics Drawing Design
Sometime editor, sound op, camera swinger. Adjunct communication instructor in Searcy, Arkansas. Proud owner of a professional deep fryer. One might say he's driven towards the pendulum.Nikon Premiere Pro Umberto Eco Academia USSR Video Editing Deep Frying Theories


text:
Display Ads
Dir. of Advertisers
Davis at the Legislature: Rain, brand fees, state aid to schools
Getting a handle on the federal budget
More opinion
Registration open for UNL ranch practicum
Governor appoints new District 4 representative to Nebraska Wheat Board
More Ag News
Click Here for theHOTTEST SHEETS!
These marriage licenses were filed with the Lincoln County Clerk Sept. 9-13.
Andrew Thomas Feeney, 26, North Platte and Courtney Marie Simants, 24, North Platte.
Glenn William Davis, 52, North Platte and Jolene Lenore Domingus, 46, North Platte.
David Keith Maier, 23, North Platte and Alexandra May Franken, 19, North Platte.
Kendal Allen Sybrant, 25, Brady NE and Brandi Lee Berumen, 25, Brady NE.
Travis Lee Potter, 34, North Platte and Fawn Lee Williams, 18, North Platte.
Flatrock Publishing, Inc.
1300 E 4th St., Suite F
North Platte, NE 69101


text:
Online signup is fast and easy!
Select the camp that most interests you and you'll be on your way in no time. Upon registration, you'll receive an instant confirmation and any additional information or instructions for the camp you've selected.
NTIS Taskforce talk about USA Baseball ....
USA Baseball NTIS Ohio Valley Camps Frequently Asked Questions:
What are the dates for the Ohio Valley Regional Championship?
Ohio Valley Regional Championship Event Dates are set!
The dates are set per age group as follows:
What are the rules for bat specifications at NTIS events?
For a complete listing of the bat specifications on all USA Baseball events including the NTIS Regional Selection Process please click here.
What is USA Baseball?
Since 1978, USA Baseball has been the national governing body for the sport of baseball. It represents the sport in the United States as a member of the U.S. Olympic Committee (USOC) and internationally as a member federation of the International Baseball Federation (IBAF).
What is the current international age determination date?
The current international age determination date states your birthday must fall on January 1 or later of a specified year to be eligible for particular age groups. The age a player reaches during the calendar year of the international competition will serve as his competition age.
Official Cancellation Policy for all USA Baseball NTIS events
Cancellations up to 8 days prior to the start of the event will receive a 50% refund of the registration fee. No exceptions will be made. The participating player or team will receive their refund within 30 days of the time of cancellation.
If my son attends a local tryout when do we find out if he made the next round or finals in the region
Check with the event coordinator who hosted the tryout, as they should issue you a code to register for the Final tryouts if you advanced. If you don't hear something within 14 days after the event call your Regional NTIS director at (610) 842-8333, but please wait until AFTER the 14 day period to contact this number. It is possible that you will receive the information via e-mail. To be informed ask at your tryout the method in which they will let you know if you advanced.
If my son makes Team Ohio Valley, does he play with them the whole year or does he only play with them for the USA Baseball NTIS event in August or September
Players who make Team Ohio Valley only play with Team Ohio Valley for the NTIS REGIONAL Event. The program is designed to make minimal impact on the players other commitments.
What teams will be playing in the Ohio Valley Regional Championships
There might be multiple teams of players per age group from the Ohio Valley Region with 18 players per team at the Final Championships. All players will have been selected through the area and state tryouts.
Are there any additional fees if my son is selected to travel to Cary, NC with Team Ohio Valley
There is a fee for each player that advances to the Regional Championship and a Team Ohio Valley Uniform Fee which is paid online through the USA Baseball and is $250.00 per player. All players will then need to book rooms and cover their travel to Cary, NC through the Ohio Valley team room blocks. Do NOT Call Hotels in Advance.
What will players wear when they travel to Cary, NC if selected to Team Ohio Valley
Players will receive a cap and jerseys when they check in for Team OHIO Valley in North Carolina. All Players will need to supply pants for the Final event, USA Baseball will determine what color pants Ohio Valley will have to wear for the event.
Does my son need to attend a Regional Tryouts or Final Regional Event to advance to the Ohio Valley Regional Championships to be named to Team Ohio Valley
This answer is YES…… All players must attend a tryout in their area or state or regional. There will be teams at each age group selected from the tryouts to participate in the NTIS Championship in Cary, NC.
Will scouts be attending the Ohio Valley Regional Championship?
Yes. Last year many college recruiters attended the Ohio Valley Regional Championship. This year we will contact over 100+ College Coaches and inform them of this prestigious talent of players.


text:
Our Recent Posts
Neuroanthropology on Facebook – Shorter Posts, More Fun!
- A short piece, but linked to the original article and a relevant interview: the...
- Christian Jarrett is no longer going to write Brain Watch for Wired, but he's go...
- Turns out that the critics of the massive European Human Brain Project to simula...
- A History of Unreason http://www.thelancet.com/journals/lancet/article/PIIS0140-...
Recent Comments
- Adult learning across cultures - Neuroanthropol... on Adult learning across cultures
- daniel.lende on Culture like Relativity
- The Dilemma with Cultural Competence | Anthropology-News on Cultural Competency in Anthropological Perspective
- John McCreery on Culture like Relativity
- Around the Web Digest: Week of March 1 | Savage Minds on Culture like Relativity
PLOS Blogosphere
Contact usDo you have a suggestion or want to submit a report? Fill out this form.
PLOS Blogs Network
- Absolutely Maybe
- All Models Are Wrong
- At the Interface
- CitizenSci
- DNA Science Blog
- Integrative Paleontologists
- Mind the Brain
- MIT SciWrite
- Neuroanthropology
- NeuroTribes
- Obesity Panacea
- On Science Blogs
- Public Health
- Sci-Ed
- The Gleaming Retort
- The Guest Blog
- The Official PLOS Blog
- The Student Blog
- Translational Global Health


text:
Tags electro, chillwave, beats, chillout, downtempo
Genres electronic, hip hop, indie, chillout, downtempo (beta)
(flag as incorrect)
Artists Onra & Quetzal (beta)
April 24, 2012
Keep Shelly In Athens, Beach House, James Blake
I love it. :)
Innocent Erotisism by palmreader
hip hop, chillwave, anything you want
Let Me Sleep by palmreader
electro pop, etherial, montreal, shoegaze/dream pop, music you didn't know
no hope kids by palmreader
noise pop, shoegaze, indie, music you didn't know
O U I J A by palmreader
Austra, Tearist, Blue Hawaii
Creepy Stuff.
witch, dark, lonesome, drone, electro


text:
Home • USB / Firewire • USB 2.0 Cables • Part Number 30U2-21000
USB OTG Adapter, OTG USB Micro B Male to USB Type A Female, USB On The Go
Product Details
- Warranty: Lifetime
- Connection: USB Micro OTG Male Plug
- Connection: USB Female Plug
- Color: Black
- Weight: 0.08 lbs
Part Number - 30U2-21000
Contact us about availability
Price: $3.16
Why Buy From Us?
- Lifetime Warranty
- RoHS Compliant
- 30 Day No Hassle Returns
- No Handling Fees
- Free Lifetime Tech Support
- Dedicated Sales Team
- Corporate Terms Available
- SSL Encrypted Shopping
USB On The Go Adapter, OTG USB Micro B Male to USB Type A Female, USB OTGThis adapter can be used with many micro B phones and tablets, basically adding a USB port. OTG stands for On-the-Go, meaning this plug is ultra-portable and can be used anywhere. This device will allow the tablet or phone to hook up to a mouse, keyboard, flash drive or an external hard-drive by a standard USB connection. Some of the more popular phones that are compatible with this adapter include: Google Nexus 5, Samsung Galaxy and the Note. Micro B male on one end and USB type A female on the other. The cable length is 4.5 inches and comes in black.
Have an opinion about this product?
Login to Write a review and share it!
Login to Write a review and share it!


text:
According to ESPN.com, the Navajo Nation has officially opposed the Washington Redskins name.
The council's committee of the whole voted 9-2 Thursday on the measure sponsored by lawmaker Joshua Lavar Butler. He says the word can have negative psychological effects on American Indians.
The statement of opposition also applies to what Butler says are disparaging references to American Indians in other professional sports franchises.
It does not apply to college or high school mascots. The mascot for at least one high school on the Navajo Nation is the Redskins.
The final authority for the measure rests with the committee of the whole.
The president of the Navajo Code Talkers Association had urged lawmakers not to approve the measure. Peter MacDonald says the NFL team's name honors American Indians.
On Friday, a United Nations human rights expert said the name of the team was a "hurtful reminder" of the mistreatment of Native Americans, but stopped short of joining in calls for the team's owner to change the name.
It looks like the foundation Redskins owner Dan Snyder created in an effort to quite down the negativeattention his franchise has received per it's name, isn't working.


text:
April 14, 2011 | Categories: dogs | Tags: chihuahua, dolorespark, jackrussell, mix | Leave a comment
Enter your email address to subscribe to this blog and receive notifications of new posts by email.
Join 6 other followers
Create a free website or blog at WordPress.com. | The Modularity Lite Theme.
Get every new post delivered to your Inbox.


text:
Shoot Cupid's arrows to grab gold and other treasures out of the sky.
Use your claw to mine for gold in your quest to build a fortune.
Trap the enemy ghosts by building walls and collecting power-ups.
Free the treacherous landscape of complex obstacles to save lives.
© 2015 GameFools.com. All rights reserved.


text:
- Mike Koozmin/The S.F. Examiner
- Different approach? Mineta San Jose International Airport, below, gives screeners more time to check bags, SFO screeners say.
Screeners working at Covenant Aviation Security say Mineta San Jose International Airport and other airports still employ the "manual slicing" function that has been disabled on SFO's X-ray screening machines.
Private security,
public concerns
A five-part series
- Employees say security unsafe
- Screeners complained to TSA
- Whistle-blowers fear reprisals
- Privatization poster child
- Is public security any better?
- Staff levels central to dispute
- Past Covenant Controversies
- Disabling a costly system
- Are other airports safer?
- Should the system be reworked?
- High-tech and high costs
They say San Jose also assigns more screeners to each machine and grants them more time to examine bags — 45 seconds compared to just 15 to 20 at SFO. But six Covenant screeners alleged that the number of screeners assigned to each machine at SFO often falls short of what the TSA requires.
"I won't fly out of SFO if I can fly out of San Jose," one screener said.
Transportation Security Administration spokesman Nico Melendez said his agency doesn't release details about its screening protocol or required machine-to-screener ratio and would not confirm whether San Jose operates differently. He said comparing machines and staffing levels at different airports is like "comparing apples and oranges," since each airport has a different configuration.
However, identifying such flaws was the purpose of a number of external audits conducted for the TSA by Battelle, a research firm with experience in security. The TSA audits all its airports as a matter of policy, screeners said.
Following one such inspection of SFO's security system, a Covenant whistle-blower recalled a Battelle auditor saying, "If I were the one to decide, I would shut down this airport."
The TSA declined to release copies of Battelle's reports. Melendez said such reports are kept secret lest they "draw a road map for those that may want to do us harm."


text:
Danish Karokhel
Article Images
MOSCOW (Pajhwok): Russia's Federal Service for the Control of Narcotics has voiced serious concern over the growth of drugs in Afghanistan, saying Kabul and the international community have failed to deal with the challenge.At a meeting with Afghan... read more
KABUL (Pajhwok): Three Independent Election Commission (IEC) workers were attacked with knives by supporters of presidential runners Dr. Abdullah Abdullah on Tuesday, an official said.The injured staffers were evacuated in an ambulance to a Kabul... read more
KABUL (Pajhwok): President Hamid Karzai said on Tuesday there were no signs of mayhem after the presidential runoff election, urging both the frontrunners to maintain friendly approach to each other during their campaigns.The president, who met... read more
KABUL (Pajhwok): On the eve of presidential and provincial council elections, the government on Friday suspended the Short Message Service (SMS) of leading mobile phone companies operating in the country.The Afghanistan Telecommunication Regulatory... read more
KABUL (PAN): President Hamid Karzai on Tuesday appointed senior career diplomat Ershad Ahmadi as deputy minister of foreign affairs for political affairs, replacing Jawed Ludin. Prior to the latest appointment, Ahmadi served as director-general of... read more
BRUSSELS (PAN): Fifty NATO and ISAF nations have promised to continue supporting the Afghanistan National Security Forces (ANSF) well beyond the 2014 withdrawal of foreign troops, the Afghan defence minister said on Friday.At the end of a two-day... read more
BRUSSELS (PAN): The International Security Assistance Force (ISAF) mission in post-2014 Afghanistan would be much smaller in size, NATO said on Friday, renewing its long-term commitment to the war-torn country. "Today, we have reinforced our... read more
BRUSSELS (PAN): Defence Ministers from 50 nations contributing to the ISAF and representatives of the European Union and the United Nations are holding an important meeting at NATO headquarters to discuss the long-term international commitment to... read more
BRUSSELS (PAN): The NATO chief on Thursday called for allies to undo defence spending cutbacks to keep the western alliance strong enough to deal with present and future challenges.Current plans for slashing the strength of the 352,000-strong Afghan... read more
BRUSSELS (PAN): With the current situation in Afghanistan on its omnibus agenda, a two-day meeting of NATO defence ministers began in this Belgian capital on Thursday.Afghan Defence Minister Gen. Bismillah Mohammadi is among the participants,... read more
BRUSSELS (PAN): The NATO secretary-general on Wednesday billed the alliance's ongoing mission in Afghanistan as a success, ruling out a security vacuum in the country after the withdrawal of foreign troops. "We will not leave behind a security... read more
BRUSSELS (PAN): Afghanistan will be an important topic to be addressed at an upcoming meeting of Ministers of Defence with ISAF contributing nations, at NATO HQ/Brussels, the western alliance said on Wednesday.That meeting will start at 09:45 on... read more
BRUSSELS/WASHINGTON (PAN): General John Allen, who served as commander of US and NATO forces in Afghanistan until early this month, has decided to retire from the military to address health issues within his family, President Barack Obama said on... read more
BRUSSELS (PAN): The United States would not specify the number of its troops staying in Afghanistan in a security deal, but the two sides would reach a broad understanding on the issue, an American diplomat said on Tuesday.However, the Bilateral... read more
NEW DELHI (PAN): Sincere international support to Afghanistan -- not meddling in its internal affairs -- is in best the interest of all countries, India's Vice-President Mohammad Hamid Ansari said on Tuesday.At a meeting with visiting Afghan... read more
NEW DELHI (PAN): India supports a homegrown solution to the 11-year-old Afghan conflict and a peace deal among stakeholders without any compromise on human rights and the constitution, Foreign Minister Salman Khurshid said on Friday.At a meeting... read more
KABUL (PAN): President Hamid Karzai on Saturday asked the media to play a proactive role in strengthening the nation by giving the people confidence in the country's future."Afghan TV channels are screening anti-Afghanistan programmes. I hope they... read more
KABUL (PAN): Kabul on Monday reacted strongly to US Special Envoy Marc Grossman's remarks that the Durand Line is an internationally recognised border between Afghanistan and Pakistan.On his arrival in Kabul from Islamabad, where he held talks with... read more
KABUL (PAN): A house was partially damaged in an explosion in the third Parwan neighbourhood of the central Kabul on Friday morning, an official said.Initial investigations showed that a water boiler exploded on the third floor of the house, police... read more
TOKYO (PAN): Before returning home, President Hamid Karzai held separate meetings with the emperor of Japan and three members of the Afghanistan-Japan Parliamentary Friendship Association in Tokyo on Tuesday.Presidential spokesman Aimal Faizi told... read more
TOKYO (PAN): A day after the Tokyo Conference, Japan on Monday promised continuing its assistance to Afghanistan during the transformation decade (2015-2025).Prime Minister Yoshihiko Noda held out the assurance during a meeting with President Hamid... read more
TOKYO (PAN): President Hamid Karzai on Monday received an honorary doctorate from the Nippon Sports Science University (NSSU) at a ceremony in Tokyo for his services to rebuilding his war-torn country.National anthems of Afghanistan and Japan were... read more
TOKYO (PAN): President Hamid Karzai on Monday said that comprehensive and frank discussions were underway between Afghanistan and Pakistan on how to bring peace and stability to the neighbouring nations.Karzai, who attended a key international... read more
TOKYO (PAN): President Hamid Karzai on Monday urged the international fraternity to extend greater help to his administration in dealing with pervasive administrative corruption in his impoverished country.A day after donors pledged $16... read more
TOKYO (PAN): A day-long international conference on Afghanistan's future and reconstruction concluded on Sunday in Tokyo, Japan's capital, with participating nations pledging more than $16 billion in conditional aid to the war-torn country over the... read more
TOKYO (PAN): Japanese Prime Minister Yoshihiko Noda said on Sunday Afghanistan was a war-devastated country that could not resolve its domestic problems without international assistance, promising Tokyo would continue to assist Kabul beyond 2014,... read more
TOKYO (PAN): Afghan civil society representatives, who met US Foreign Secretary Hillary Clinton on the sidelines of the Tokyo Conference, on Sunday called for trilateral monitoring of the aid pledged at the international gathering.A spokesman for... read more
TOKYO (PAN): A trilateral meeting among senior diplomats from Afghanistan, Pakistani and the United States was held on the periphery of a 70-nation conference on aid to Afghanistan in Tokyo on Sunday.The three foreign ministers, Special US... read more
TOKYO (PAN): A decade of sacrifices of cash and blood would be lost if Afghanistan did not receive continued international aid after the withdrawal of foreign troops in 2014, the UN secretary-general warned on Sunday. Ban Ki-moon told participants... read more
TOKYO (PAN): The United States Secretary of State Hillary Clinton said the Obama administration will seek Congress's approval to sustain its annual funding to help Afghanistan until 2017.Speaking at the Tokyo Conference on Sunday, Clinton told donor... read more
TOKYO (PAN): The Tokyo conference kicked off in Japan on Sunday with international donors trying to raising billion of dollars as civilian aid to sustain Afghanistan slow-paced economy.Top officials from some 70 countries are gathering in... read more
TOKYO (PAN): On the eve of a major international donors' conference, important meetings on Afghanistan took place in the Japanese capital on Saturday.A UNHCR symposium on "A Solutions Strategy for Afghan Refugees: Linking Humanitarian Action to... read more
KABUL (PAN): Seeking billions of dollars in civilian aid, President Hamid Karzai on Saturday left for Japan at the head of a high-level delegation to attend the International Tokyo Conference scheduled for July 8.Representatives from nearly 70... read more
KABUL/ISLAMABAD (PAN): Ending days of uncertainty, NATO has invited Pakistan to the Chicago summit, slated for May 20-21, a spokesman for the alliance senior civilian representative in Afghanistan said on Tuesday.Dominic Medley confirmed to Pajhwok... read more
KABUL (PAN): The Afghan government should spearhead the ongoing reconciliation process in the country, the United Nations special envoy for Afghanistan said on Wednesday.At his maiden news conference as head of the United Nations Assistance Mission... read more
BERLIN (PAN): President Hamid Karzai on Tuesday conceded that Pakistan could play a crucial role in the success of his government's peace campaign, particularly in terms of persuading the Taliban to come to the negotiating table.Afghanistan and... read more
BONN (PAN): In return for continued international assistance, Afghanistan on Monday promised to intensify efforts at combating corruption, promoting the rule of law and upholding women's rights.In a communiqué issued at the end of the day-long Bonn... read more
BONN (PAN): India's External Affairs Minister SM Krishna on Monday pledged to help Afghanistan combat terrorism and rebuild the war-ravaged nation..Speaking at a crucial conference on Afghanistan's future in Bonn, Krishna said: "India is willing to... read more
BONN (PAN): Kazakhstan on Monday promised it would continue to promote restoration of peace in Afghanistan, with an emphasis on social and educational programmes and projects."Humanitarian assistance is regularly provided in the form of food and... read more
BONN (PAN): Two women carrying a banner with slogans against the foreign military presence in Afghanistan briefly disrupted the Bonn Conference in Germany on Monday.The women entered made their way to the second-floor press gallery soon after US... read more
BONN (PAN): A female civil society representative, Seli Ghafari, has criticised the justice and judicial system in Afghanistan as corrupt and fragile.Addressing a gathering discussing women rights and peace in Afghanistan in the German city of Bonn... read more
BONN (PAN): President Hamid Karzai on Monday promised his government would press on with efforts, briefly stalled by the High Peace Council head Prof. Burhanuddin Rabbani's assassination, at pursuing peace with the Taliban.For the reconciliation... read more
BONN (PAN): The second Bonn Conference on Afghanistan's future, being attended by representatives from more than 115 countries and international organisations, was opened by the German foreign minister on Monday.The Afghan-led gathering began with a... read more
BONN (PAN): The unity of the international community in supporting Afghanistan over the long haul was essential for the country's stability and preserving the gains made over the past decade, UN Secretary-General Ban Ki-moon said on Sunday.At a... read more
BONN (PAN): The second Bonn Conference on Afghanistan's future, being attended by representatives from more than 115 countries and international organisations, is set to begin in Germany on Monday.Global political, economic and military assistance... read more
BONN (PAN): Representatives of the Change and Hope Coalition, the main Afghan opposition grouping, on Sunday met delegates on the eve the Bonn Conference on Afghanistan's future direction.Former deputy minister of foreign affairs, Mahmud Saiqal,... read more
BONN (PAN): Afghans living in Europe, although not optimistic about the outcome of this week's international conference in Germany on Afghanistan's future, have urged the summit participants to help remove hurdles hindering the ongoing peace process... read more
BONN (PAN): Thousands of people are expected to take to the streets in Bonn, once the capital city of Germany, on Saturday against a key international summit on Afghanistan's future.The demonstration would be staged in the afternoon, local time, by... read more
BONN (PAN): Three representatives of the Change and Hope Coalition, the main opposition grouping, have arrived in Germany ahead of the upcoming Bonn Conference on Afghanistan's future direction.Former minister of foreign affairs, Mahmud Saiqal,... read more
BONN (PAN): The Bonn Conference, penciled in for Dec. 5, will mobilise the global fraternity to support Afghanistan beyond 2014, when all foreign troops are scheduled to pull out of the country, the German foreign minister said on Friday."We seek a... read more
BONN(PAN): German and Afghan journalists' organisations have called for delegates of the 2nd Bonn Conference, scheduled for Dec. 5, to pay proper heed to the development ofAfghanistan's nascent media sector.The call came at a two-day seminar... read more
KABUL (PAN): A non-governmental organisation (NGO) is trying to conduct women's rights awareness programmes through religious leaders and distribution of booklets on the subject in Afghanistan.A two-year pilot programme, featuring 20 prayer leaders... read more
NEW YORK (PAN): The New Galleries from the Art of Arab countries, Turkey, Iran, Central and South Asia and Afghanistan were displayed on Monday in the Metropolitan Museum of Art in New York.The 15 new galleries, including five historical objects... read more
KABUL(PAN): Nine crewmembers were believed to have been killed after a cargo plane crashed into mountains east of Kabul on Tuesday, an official said. The aircraft, under contractual agreement with foreign troops, was en rout to Kabul airport from... read more


text:
It's not the ritz- but you know what? It's clean, the staff are helpful, the wifi is good, the rooms were really lovely and quaint, the shower was AMAZING (always important for me), also it's only a 15 min easy walk into the heart if bologna. You get what you pay for- but in this one you won't be disappointed.... More
- Reservation Options:
- TripAdvisor is proud to partner with Expedia, Booking.com, Venere, Hotels.com, Travelocity, Priceline and Odigeo so you can book your Blumen Hotel reservations with confidence. We help millions of travelers each month to find the perfect hotel for both vacation and business trips, always with the best discounts and special offers.
- Also Known As:
- Blumen Hotel Bologna


text:
Ruby On Rails Showdown with Java Spring/Hibernate 555
from the battle-of-the-overhead dept.
We've improved Slashdot's video section; now you can view our video interviews, product close-ups and site visits with all the usual Slashdot options to comment, share, etc. No more walled garden! It's a work in progress -- we hope you'll check it out (Learn more about the recent updates).
doesn't optimized usually mean faster?
I'd give you an answer, but I haven't been able to fully test out my optimized Gentoo box yet -- it's still compiling.
Just watch, it's impossible to have an intelligent discussion between the two groups.
Is that so, Mr. Poopyhead?
It is the quality rather than the quantity that matters. - Lucius Annaeus Seneca (4 B.C. - A.D. 65)


text:
Everything tagged
Latest from The Spokesman-Review
Rep. Doc Hastings leaving Congress; enviros say 'good riddance'
CONSERVATION — Some conservationists didn't hide their happiness to hear that Rep. Doc Hastings, R-Wash., is retiring after 20 years in Congress, an unexpected announcement that drew both cheers and jeers Thursday in the nation's capital.
As chairman of the House Natural Resources Committee, he's been a barrier to many efforts championed by environmentalists, especially those who objected to his persistent moves to open more public lands to development and to change laws dealing with endangered species, among other things.
"It's really good riddance," said Athan Manuel, director of the lands protection program for the Sierra Club.
Click "continue reading" for the full story from the McClatchy Washington Bureau.
Holmquist Newby may run for Congress
OLYMPIA — State Sen. Janea Holmquist Newbry said she has formed a committee to explore whether to run for the congressional seat opening up in Central Washington's 4th District.
Holmquist Newbry, R-Moses Lake, said she plans to visit "with key leaders throughout the district… to assess the viability of my candidacy."
U.S. Rep. Doc Hastings announced Thursday morning that he would not seek re-election to the seat he has held for 20 years. Holmquist Newbry said shortly afterwards she had not made a decision on whether to run "at this time."
Friday she said she has formed an exploratory committee with several key advisers, including Yakima Mayor Micah Cawley. Under federal election law, a person can receive and spend money before becoming an official candidate by forming an exploratory committee.
First elected to the House in 2000, Holmquist Newbry was elected to the Senate in 2006, where she now serves as the chairwoman of the Commerce and Labor Committee. She recently removed her name from the list of senators on the website for the Majority Coalition Caucus, telling the Seattle Times she was protesting decisions by some of the more moderate members of the caucus.
The 4th is a solidly Republican district that routinely gave Hastings at least 60 percent of the vote in his re-election campaigns. Also considering the race is rancher Clint Didier, an unsuccessful candidate for U.S. Senate and state lands commissioner, who may make an announcement on Monday.
Columbia River Treaty hearing set in Pasco Monday
RIVERS — Discussions on revising the Columbia River Treaty are picking up, as the U.S. House Natural Resources Committee plans a field hearing Monday, Dec. 9, in Pasco to learn about regional impacts of the treaty with Canada.
Changes in the treaty could have profound impacts on hydropower management and fishing.
Rep. Doc Hastings, R-Wash., chairman of the committee, has concerns about the upcoming renegotiation of the treaty and the United States' draft recommendations for possible changes.
He scheduled the hearing for 9 a.m. in the Pasco City Council Chambers, 525 N. Third Ave.
Read on for more from the Associated Press:
Is Doc Hastings eying House Oversight chair?
Politico's Rachel Bade reports this morning U.S. Rep. Doc Hastings, R-Pasco, may be considering a slot as chairman of the House of Representative Oversight Committee, to be vacated by Rep. Darrell Issa, R-Calif., at the end of next year.
"Call this Boehner ally and personal friend the wild card," Bade writes, introducing the nine-term House Republican who was the last to defeat current Washington Gov. Jay Inslee in a Congressional race when he wrangled the District 4 seat from Inslee, the incumbent, in 1994.
To read the rest of this item, or to comment, continue inside the blog.
Hastings introduces permanent sales tax deduction bill
Another volley has been launched at the temporary status of the sales tax deduction on federal income tax returns. Once again, it comes from a Washington lawmaker.
Rep. Doc Hastings, R-Pasco, filed a bill with Rep. Jeff Miller, R-Fla., Tuesday that would make permanent a sales tax deduction written into the federal tax code. Current law, solidified in last year's fiscal cliff deal, extends the deduction through next year only. Meanwhile, taxpayers enjoy the income tax deduction on a permanent basis.
Like others before him, including Sen. Maria Cantwell, D-Wash., who authored similar legislation across Capitol Hill in January, Hastings argued the issue is one of fairness for states that collect no income tax.
"Residents of states that do not collect income taxes, like my home state of Washington, should be allowed to continue to deduct their state sales taxes from their federal income tax obligations each year without relying on short term extensions of the law," Hastings said in a prepared statement Tuesday announcing the bill.
Hastings wants public to know costs of saving salmon
RIVERS — The House Natural Resources Committee held a hearing in Pasco toda on "save our dams" legislation introduced by Congressman Doc Hastings.
The Tri-City Herald reports the legislation would prohibit federal money from being used to remove hydropower dams without specific authorization by Congress.
The bill also would:
- Prevent federal grants from being used by groups suing the federal government to breach dams.
- Make it clear that hydropower is a renewable energy source, just like wind and solar power.
- Tell consumers about how much laws to protect fish and wildlife add to their power bills.
Shouldn't the bill also assure the public knows the value of fish and wildlife lost to power production, and the associated health issues, and the cost to subsidize the barging industry and the federal cost to replace the dams and deal with the silt issues that will come to a head in the next few decades?
There's a cost to everything. To peg fish and wildlife as the villain is disingenuous.
Rep. Hastings targets Endangered Species Act
ENDANGERED SPECIES — Rep. Dod Hastings, R-WA, says he's ready to rewrite the Endangered Species Act, according to this McClatchy report.
Did you know members of Congress can be jerks?
It's always entertainting the way certain politicians talk about environmental issues. This weird exchange in Congress is no different. The scene is a committee hearing on drilling in the Arctic National Wildlife Refuge. Rep. Don Young (R-Alaska), a notorious drilling advocate and Rep. Doc Hastings (R-WA), a huge proponent of anti-environmental legislation, battling with historian Douglas Brinkley.
YOUNG: I will tell you if you ever want to see an exercise in futility, it's this hearing. That side's already made up its mind. This side has already made up its mind. And I call it garbage, Dr. Rice, that comes from the mouth…
BRINKLEY: Dr. Brinkley. Rice is a university. I know you went to Yuba College and you couldn't graduate.
YOUNG: I'll call you anything I want to call you when you sit in that chair … You just be quiet.
BRINKLEY: You don't own me. I pay your salary.
YOUNG: I'll tell you right now …
BRINKLEY: I work for the private sector. You work for …
HASTINGS: Mr. Brinkley, do you want to continue to sit in this panel?
BRINKLEY: Yes.
HASTINGS: OK. Then please follow the rules.
Yikes. Of course, this goes into the Congressional Record forever which is pretty awesome. But after the jump is the most telling part from Rep. Young.
GOP plan would open more public land to off-road vehicles
PUBLIC LANDS — Congressman Doc Hastings, R-WA, the chairman of the House Natural Resources Committee, is promoting a Republican plan that would remove restrictions on motorized access to 43 million acres of public land nationwide.
A tip of the hat to the Backcountry Hunters and Anglers organization for trekking to Washington, D.C., to let Congress know that's a lame idea.
Read the story from McClatchy Newspapers.
Rep. Hastings vows to block breaching Snake River dams
RIVERS — Speaking to the Pasco-Kennewick Rotary Club on Wednesday, Congressman Doc Hastings, R-WA, said he'll use his position as chairman of the House Natural Resources Committee to block any bills related to breaching lower Snake River dams.
Hastings said salmon runs are recovering under current management practices and dam breaching is the last resort.
The Tri-City Herald reports Hastings as saying he's concerned that tearing down any Snake River dam puts every other dam at risk.
Environmentalists favor removing dams to restore Snake River salmon runs.
WA congresspersons to Pentagon: Keep tanker timeline
Five members of Washington's congressional delegation joined an effort to keep the Pentagon from delaying its selection the builder of the next air refueling tanker by asking Defense Secretary Robert Gates not to extend the timeline for making that choice.
Without mentioning either company by name, they are supporting the Boeing Co., and trying to close out rival Airbus.
Republican Reps. Cathy McMorris Rodgers and Doc Hastings, and Democrats Jay Inslee, Adam Smith and Rick Larsen are among 16 members of the House urging Gates not to vary from the 75-day selection deadline announced in February. Their stated reasons include eight years of delay already in replacing the KC-135s, and the additional costs to taxpayers.
The KC-135, which was designed by Boeing in the 1950s and built through the early 1960s, is the backbone of the U.S. Air Force tanker fleet and the plane flown by the 92nd Air Refueling Wing and the Washington Air National Guard's 141st Air Refueling Wing, both based at Fairchild Air Force Base.
Finding a replacement for part of the KC-135 fleet started some nine years ago, and has been marked by fraud, collusion, political bickering and pandering, mistakes and missteps. Meanwhile, the 135s keep flying in two war zones and for a variety of other military missions around the globe.
The process to select a new tanker is a subject of intense interest, the members of Congress wrote, but "the need for new tankers is long overdue."
A consortium that included Northrop Grumman and EADS, the manufacturer of Airbus, beat out Boeing for an estimated $40 billion contract in early 2008. That award was thrown out a few months later after Boeing protested and the Government Accountability Office found problems with the selection process. New specifications were announced in February and Boeing notified the Air Force eight days later it would enter a new bid, again using a version of its 767 jetliner.
Four days later, Northrop Grumman said it wasn't entering the competition. In late March, however, EADS said it would submit a proposal if the deadline was extended and "there is a fair chance to win."


text:
Fortunata Kasege, Houston, Texas, diagnosed in 1997
The best one was the first time when I told my story in public. I remember it.
After my father died, it seemed like the end of the world. But I decided to go out and share my story. I decided to talk about awareness and maybe somebody will be touched by this, so it isn't all tragedy.
I got this invitation to go to Kentucky. They had a fundraiser gala for World AIDS Day last year. That was my first time to go up there and tell my story in public. I remember after I finished, everybody stood up and they remained standing there for a few minutes. They were clapping constantly.
I remember looking, and wondering, what is so special about this thing? I was overwhelmed; they had a standing ovation for me -- that was probably the only one that I can remember that was an over-the-top reaction. I thought, "Here I am, telling my business, right in front of people. I don't know how they're going to react." The outcome was remarkable. They were coming after that to talk to me and thank me for sharing my story and encouraging me.
The pastor from the community said, "People here, they're very uptight, and very conservative thinking about this disease. They have their way of thinking about the people who have this disease. You put a new face on it, and thank you. We want you to come back and speak to our church."
No comments have been made.
More Slideshows Like This
|Am I Too Worried About Getting HIV?
|An HIV Doctor Tells You if Serodiscordant Couples Need to Use Condoms
|Tips From Experts on HIV and Stomach Woes
|Inflammation Persists Despite Very Early HIV Treatment
|Spotlight on HIV Stigma & Discrimination
|When Words Work Against Us: The Language of HIV Stigma
|An HIV Doctor Tells You if Serodiscordant Couples Need to Use Condoms
|In MSM, PrEP Stops HIV, Not Other STIs. Does It Matter?
|When Words Work Against Us: The Language of HIV Stigma
|Tips From Experts on HIV and Stomach Woes
|Aging GRACIE-fully
|How Transgender People Are Fighting Stigma, Demanding HIV Justice
HIV Tools You Can Use