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ABC News' David Chalian Reports:
As Congress settles back into Washington for another work period and with the likelihood of a bloody battle of Supreme Court nominee Sonia Sotomayor seeming remote, health care activists are preparing for their issue to move to the center ring of politics.A coalition of liberal and progressive groups announced today plans to spend "at least $82 million this year on working to support President Obama's plan and get a guarantee of quality affordable health care for all passed this year and in this Congress," according to Richard Kirsch, national campaign manager for Health Care for America Now (HCAN).The group's campaign plan calls for spending most of those resources on grassroots organizing and paid advertising including television ads. Mr. Kirsch boasts of having more than 140 organizers on the ground in 40 states at current count."With what can't compete inside with the armies of lobbyists, we can compete where it really matters — at the grassroots," said Kirsch.The coalition's initial efforts are focused on pressuring Congress to produce a health care reform bill that includes the availability of a public insurance plan that will compete with the various private plans in the market place. Opponents of the public option claim it will be near impossible for private insurers to compete with a government plan driving many of those private insurers out of business. There was little discussion at the campaign launch today about how to pay for reforming the system.Former DNC Chairman Howard Dean once again said that a health care reform plan without a public option would be a waste of money and not the offer the kind of fundamental reform proponents believe is required. "The idea that the public option is going to somehow wipe out private insurance is historically incorrect," Dean said. "Will there be changes in the insurance market? Yes, that's the purpose of having the public option. Insurance companies will have to compete." Dean said in answering his own rhetorical question. Dr. Dean also said he believes a sea change has occurred in American politics that allows for this moment to be the time when fundamental health care reform can occur."More people who are under the age of 35 years old cast a ballot in [the 2008 presidential race] than people who are over 65. That has never happened in my lifetime," said Dean. "We have seen a sea change in this country. A complete change. A generation which is center-left. They happen to be conservative probably about money, but in terms of social values and social programs and the kind of agenda on the environment, and for health care, and for equal rights for every single American, this is a center-left nation."The proponents for a public insurance option as part of health care reform will not have the battlefield to themselves, of course.Over the weekend, the Rick Scott funded "Conservatives for Patients' Rights" launched a 30-minute infomercial aimed at debunking the public option by highlighting stories of long waiting times patients experienced in the Canadian and British government sponsored systems."Anything that seems too good to be true like national health service truly is," Scott says in the infomercial.
Health Care Warriors Take to the Battlefield
ABC News' David Chalian Reports:


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Newton News
Local news for Newton, NJ continually updated from thousands of sources on the web.
County residents can ski for free today
Tuesday Mar 24 | New Jersey Herald | Comment?
Food Assistance Program available in April at Project Self-Sufficiency in Newton
Monday Mar 23 | The Jersey Journal | Comment?
Hackettstown man charged with DWI
Monday Mar 23 | New Jersey Herald | Comment?
Ready to move?: Everything you need to know about Lafayette Township
Sunday Mar 22 | NJ.com | Comment?
Investigation continuing into fatal crash in Wantage
Sunday Mar 22 | New Jersey Herald | Comment?
The Email Address Debacle: Did Hillary Do Something Wrong?
politics 228
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topix 247
46 Shocking Photos Of Stars Without Makeup
Fire at Newton church extinguished
Saturday Mar 21 | New Jersey Herald | Comment?
Court date postponed in Sparta ice slick case
Friday Mar 20 | New Jersey Herald | Comment?
Parade steps off Saturday in Newton
Ginnie's House promotes awareness of child abuse
Official: 2 New Jersey boys taken by father found in Florida
Thursday Mar 19 | KNUS-AM Aurora | Comment?
Jim Brickman bringing Platinum Tour to Newton
Landing woman charged with DWI
Tuesday Mar 17 | New Jersey Herald | Comment?
The Three Puppeteers
Monday Mar 16 | New Jersey Herald | Comment?
'Top 100' guitarist Gary Hoey to play at Newton Theater
Monday Mar 16 | The Advertiser News... | Comment?
Branchville driver accused of hitting pedestrian in Newton
Monday Mar 16 | New Jersey Herald | Comment?
NAMI presents 'Coping with Depression' program, April 2, in Newton
Sunday Mar 15 | The Jersey Journal | 1
Updated: Tue Mar 24, 2015 10:29 pm
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OP Junior Member
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I've just published the first beta of a new word puzzle game called Wordismic and I'm looking for testers. People who like word games are obviously especially welcome.
This video gives an idea of the game in action:
To get the app you need to be a member of the XDA Android App Testers Google+ Community and join as a tester in the Play Store:
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8 Items Found
- Dom De l'Eveche Bourgogne Blanc 2011 Product #29283 (In Stock)
This is white Burgundy in its most pure and unassuming form - and at a very unassuming price, too! The Joussier family has only owned Domaine de l'Eveche for 30 years, but they've already established themselves as one of the most talented, quality-oriented, estates in the Cote Chalonnaise. Their Bourgogne Blanc is 100% Chardonnay from limestone rich vineyards and vines 15-30 years old. The ripe, carefully selected, fruit ferments and ages entirely in tank to preserve the fresh fruit flavors and avoid masking these fine sites' classic minerality.
You'll find aromas and flavors of fresh peaches, nectarine, green pear and apple at the wine's core with accents of citrus and fruit blossom. With air, pretty notes of warm spice and hazelnut peak out and snappy acids help drive mineral, pear, nut, and citrus flavors through the long finish. The balance here is perfect and the structure pristine, so you can drink this on the warmer side to really let the flavors shine with no loss of refreshment or vigor. Delicious, but limited.
- Dom Michel Sarrazin Cremant De Bourgogne NV Product #29289 (In Stock)
The Sarrazin brothers craft their Cremant from 80% Pinot Noir and 20% Chardonnay harvested early (to preserve fizz-friendly acidity) from 30 year-old vines just outside the village of Givry. They blend the tank-fermented base wines and then perform secondary fermentation and aging on the lees in bottle (just like in Champagne). Once the wine has picked up the distinctive notes of toast, bread dough, and grilled nut only Methode Champignois can deliver, the bottles are riddled, disgorged, and topped up with a very modest 6 grams/liter dosage – enough to round out the acids but still leave the wine crisp and dry on the finish.
The folks at locally-based International Wine Review said of the previous release, "it is richly flavored and a very attractive alternative to non-vintage Champagne." The aromas are luxurious: rich scents of toasted nut, brioche and bread dough over golden apple fruit. The texture is creamy and luxurious, too, with generous flavors of peach, pear, apple, toasted hazelnut and tangy butter. A red cherry streak peaks out on the back and continues on through the buttered nut and white fruit flavors of the long, fresh, finish. Better than most $40 Champagnes and fine value at the $25 regular price.
- Dom Cordier Vire Clesse Vielles Vignes 2012 Product #29347 (In Stock)
The Vire Clesse appellation was created in 1999 in recognition that this limestone-rich stretch of vineyards was of higher quality than most Macon sites, if not quite up to the level of Pouilly Fuisse. By law, all Vire Clesse wines must be bone dry, giving the best examples (like this one) an almost Chablis-like cut and vibrancy.
This beauty opens with a wonderful saline nose of crushed rock, crisp apple, stone fruit and fresh mint. The palate is sophisticated, luscious, and quite complex, with layered flavors of apple, apricot, and pineapple fruit framed by more mineral and a touch of lees. Finishes pure, clean, and long with salty caramel, fresh fruit, and white flower notes and a mouthwatering burst of fresh pineapple with a touch of chalk left behind. Utterly delicious and compelling now and for the next 2-3 years.
"The 2012 Viré Clessé Vieilles Vignes has a well-defined, precise bouquet with fragrant white peach and persimmon notes that remain focused and taut. The palate is well-defined and crisp with brisk acidity and very fine tension to the slightly austere finish that deserves a few months in bottle. This is well-crafted and might well repay just a few months in bottle if you choose to do so. Drink 2014-2019."Wine Advocate 89 points
- Langlois Pere Et Fils Cassis Nv Product #28099 (In Stock)
The first thing you'll notice about this delicious creme de cassis is its almost neon color, a gorgeous, glass-staining magenta. Made from super-ripe cassis berries grown on the Langlois estate, this is sweet, rich and lucious, to be sure, but also has an intriguing undercurrent of something leafy and herbal that keeps it from becoming cloying. If you've never had a real creme de cassis, try adding a few drops to sparkling wine for a Kir Royale you won't forget!
- Langlois Pere Et Fils Mure Nv Product #28100 (In Stock)
Wild blackberries harvested from around the city of Nevers in Burgundy make this unctuous liqueur, traditionally drunk with Bourgogne Rouge for a sort of brunette sister to the Kir called a Berrichon. After the berries are harvested, they are frozen to break down their cell walls, making for more concentrated flavors. Combining the sweetness of ripe fruit with more earthy, musky aromas than the more common creme de cassis from the same region, this is unusual and delicious.
- Dom Michel Sarrazin Mercurey La Perriere 2012 Product #28815 (In Stock)
A winner of a red Burgundy that delivers the perfect blend of luscious Pinot Noir fruit, toasty oak, and authentic Burgundy earthy minerality. The Sarrazin brothers grow this in the fine La Perrriere vineyard, just next to the 1er Cru Le Clos L'Eveque, prick it ripe, and give it a luxurious stay in 75% new French oak. In 2012, it's wonderfully rich and concentrated, but never rough or rugged and has absorbed the wood beautifully. Aromas and flavors of sweet red cherry, red plum, and raspberry with accents of crushed violet and smoke. The fruit is nicely sweet and fresh - more than enough for any Willamette Valley Pinot drinker - but with a touch of Burgundy's own iron ore and warm stone minerality. You'll love how the fruit gets more sweet and fresh with air without losing the focus of the firm finish. A classy but easy-to-drink Burgundy that shines at table or for a crowd.
"The 2012 Mercurey La Perriere Village displays perfumed red cherry and plum aromas. On the palate it has excellent depth of flavor and layered flavors with a firm tannic finish. This wine should provide drinking pleasure until 2019 or longer." i-Wine Review 90+ points
- Dom Cordier Pouilly Fuisse Vielles Vignes 2010 Product #29346 (In Stock)
A fine Pouilly Fuisse should be rich, generous, and just a touch exotic - and this fantastic 2010 from old vines delivers all of that and more. The aromas are just classic: ripe pineapple, fresh peach, creamy butter, and sweet mint all burst from the glass. The palate is rich and buttery with generous flavors of apricot, passion fruit, citrus, grilled pineapple and creme fresh. The flavors completely coat your palate but avoid heaviness as tangy pineapple, passion fruit, lemon zest, mint and crushed stone flavors linger over the geneours, mouthwatering, finish. Still a baby with at least 5-6 years of cellar development in front of it, but hard to resist for the rich fruit right now.
"Sweet Spot Outstanding. The big, rich and powerful medium-bodied flavors exude a palate staining dry extract that pushes the otherwise firm acid spine to the background but not so much that the complex and impressively long finish loses its focus. This should be terrific and offer an extended cellaring potential if desired yet the abundance of sap will allow this to be enjoyed young."Allen Meadows 90-93 points
"Full-bodied with sunny warmth, this golden wine has a pale earthiness touched by some exotic notes of kumquat and mango. Seashell minerality integrates the alcohol into the fruit. The flavors last, ready to serve with roast sea bass."Wine & Spirits 90 points
"A broad, powerful white, evoking fennel, grapefruit, pineapple and apricot flavors tinged by oak spice. Well-structured and long, with a mineral aftertaste. Drink now through 2018."Wine Spectator 91 points
- Thibault Liger-belair Nuits St George La Charmotte 2012 Product #29164 (In Stock)
"An intensely floral character adds breadth to the very fresh, cool and restrained aromas of various red berries, earth and humus. Here too there is a lovely mouth feel to the detailed and energetic middle weight flavors that possess excellent length and almost no rusticity."Allen Meadows 88-91 points


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Samuel Dalembert Pictures
SAN ANTONIO, TX - APRIL 30: Tiago Splitter #22 of the San Antonio Spurs shoots against Samuel Dalembert #1 of the Dallas Mavericks in Game Five of the Western Conference Quarterfinals during the 2014 NBA Playoffs at the AT&T Center on April 30, 2014 in San Antonio, Texas. NOTE TO USER: User expressly acknowledges and agrees that, by downloading and/or using this photograph, user is consenting to the terms and conditions of the Getty Images License Agreement. (Photo by Chris Covatta/Getty Images)


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St. Cloud rides fast start past Huskies
Will Craig blasted a pair of home runs, and the St. Cloud Rox blasted the Duluth Huskies 10-3 in a Northwoods League game Saturday in St. Cloud.
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Cancer Health Center
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- New Childhood Leukemia Drug Approved
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- Childhood Cancer May Raise Physical Challenges
- Childhood Cancer Up in Europe Since the 1970s
- Childhood Cancer and Psychiatric Problems
- Engine Exhaust Linked to Childhood Cancers
- Childhood Leukemia Increases Risk of More Cancer
- Childhood Cancer: Health Risks Linger
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Canstar Community News - ONLINE EDITION
A royal victory
Congratulations to the River East Royals bantam A1 hockey team, winners of the Stonewall Bantam Tournament on Dec. 16. After facing teams from Gimli, Beausejour and Stonewall, the Royals squared off against Fort Garry Red in the gold medal game. Justin Turman scored with 39 seconds remaining after a great pass from Evan Paulley to break a 1-1 tie, giving the Royals the victory.
Front row (from left): Ian Regier, Ryan Kowalchuk, Nolan Yakimowski, Taylor Engel, Bryson Rochelle, Zach Fincaryk, Riley Schmidtke, Zac Cardno.
Second row: Evan Paulley, Justin Turman, Brett Brydges, Keiran Desbois, Colin Piche, Stefan Kushka, Ryan Shaski.
Back row: Coaches Rick Bellemore, Scott Cardno, Dylan Bellmore.
Missing: Cooper Kolotylo.
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@TimesWPG: The Garden City Aces struck gold twice on Sunday. winnipegfreepress.com/our-communitie… #cbn 3:28 pm on Mar 27
@TimesWPG: Four northwest Winnipeggers up for @YWinnipeg Women of Distinction Awards winnipegfreepress.com/our-communitie… #cbn 3:27 pm on Mar 27
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@TimesWPG: Giles twins led Sisler to a provincial basketball crown earlier this week. winnipegfreepress.com/our-communitie… #cbn 3:23 pm on Mar 27
@canstareditor: RT @CanstarHeadline: New homes to be built next to Blumberg Sports Complex in RM of Headingley. winnipegfreepress.com/our-communitie… #cbn 2:09 pm on Mar 26
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This jacket is nice and toasty on the inside and keeps out the cold during my winter training runs. I wouldn't imagine wearing this while it's anything more than a drizzle outside because there's no hood but it's great at blocking the wind and keeping me warm.
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(January 20, 2009: Davison, MI 48423) In Tuesday's non-league outing, the Flint Southwestern Academy Knights (Flint, MI) basketball squad beat the host Davison Cardinals (Davison, MI), by a margin of 67-49.
The Knights (6-3) will now prepare for their outing against Northern (Flint, MI). The Vikings go into the Saginaw Valley outing with a 4-5 record. In their last outing, Northern was topped by Carman-Ainsworth (Flint, MI), 69-67, in a non-league outing.
The Cardinals now own a 4-4 record. They take the field next when they travel to Carman-Ainsworth for a Big Nine outing on Friday, January 23. Davison will encounter a Cavaliers squad coming off a 69-67 non-league win over Northern (Flint, MI). The Cavaliers record now stands at 4-3.
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Jonathan's Archive
James Franco in Joe's Jeans on Flaunt Magazine
James Franco covers Flaunt Magazine with two covers (this is the suitable for work one). He wore a head-to-toe Joe's Jeans look. He wore The Shirt and a pair of the Classic Fit in Heche for the cover.
James is such a mellow guy. I think that may be why the fashion mags love him so much. He was named the face of Gucci's made to tailor line and has been a muse for Frida Giannini for quite some time. Thinking about it, James seems to be quite a busy person. He seems to balance school, acting, and modeling work very well. He's either hiding stress really well or has none. You can click here to purchase these jeans at Joe's jeans for $158.
Robert Pattinson in J Brand
Robert Pattinson was caught during his arrival at the Roissy Charles-de-Gaulle Airport in Paris, France. He wore a pair of J Brand Kane jeans in Boone. Seeing these from the front, I think they fit Robert really well. They fit, but have some bagginess to them.
How many of you readers are excited to see the new Twilight movie in November? It seems like a lot will be happening in this one. The trailer just seems so suspenseful this time around. You can click here to purchase men's J Brand Jeans at Singer22.com.
Ashlee Simpson in James Jeans
Ashlee Simpson was caught with her family arriving to LAX to catch a flight out to New York. She wore a pair of James Twiggy Leggings in Clean Black. The Simpson clan are taking a trip to New York to do some work on the new tween collection for Jessica's Jessica Simpson Collection. What are your thoughts of having Ashlee head the younger line for Jessica?
That Veda Supreme jacket has to be one of the sickest motorcycle jackets that I have seen. When I first saw these pictures, that was the first thing that caught my attention. It is just so amazing. The perfect color combination on it and it goes so well with her whole look. You can click here to purchase these jeans at Singer22 for $114.
Khloe Kardashian in Current/Elliott
Khloe Kardashian not in Rag & Bone? This is the first time we have seen her in another brand in a while. She was caught going to dinner with husband Lamar Odom at Nobu Restaurant in Hollywood, California. She wore a pair of the Current/Elliott Ankle Skinny Leather pants in Fig. Thanks to her stylist Monica for confirming who they are by.
I fell for these pants ever since I first saw them. They are a great new take on leather pants. The color is so cool. I have to say that I am liking colored leather more and more, especially brown, red, and purple tones. They are bold enough to stand out, but not out stage the fact that they are leather. It adds a bit more cool to the already cool that leather has. You can click here to purchase these pants at Singer22 for $855.


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Electrical and electrochemical end-column detectors are provided in which the sensing microelectrode is placed at the outlet of the separation capillary. The novel design minimizes the electrical interference caused by the applied high voltage during CZE separations, and provides for detectors that are easy to construct, sensitive, reliable, and easy to operate.
1. A capillary electrophoresis apparatus comprising:
a separation capillary having an outlet end through which an electrophoretically separated component moves;
a reservoir containing a buffer, the outlet end of the separation capillary being submerged in the buffer;
a sensing electrode, positioned adjacent to and outside the outlet end of the separation capillary in the buffer, that responds to the component as it flows out of the separation capillary; and
a reference electrode positioned in the buffer;
means for passing a current between the electrodes; and
means for measuring change in an electrical parameter between the two electrodes in response to the component.
2. An apparatus as defined in claim 1 wherein the distance between the sensing electrode and the outlet is sufficiently small so that as the component flows out of the separation capillary the component is not substantially diluted by buffer.
3. An apparatus as defined in claim 1 further comprising a second capillary into which the sensing electrode is inserted, wherein said second capillary is positioned adjacent to and outside the outlet end of the separation capillary to define an eluent gap through which the component flows.
4. The apparatus of claim 3, wherein the eluent gap is in the range of about 1 to 5 microns in dimensions.
5. The apparatus of claim 1, wherein the sensing electrode is placed at a distance in the range of about 1 to 5 microns from the outlet end.
6. An apparatus as defined in claim 1 further comprising means for establishing an eluent gap through which the component flows.
7. An apparatus as defined in claim 1 said reference electrode being a grounding electrode wherein said parameter is the electrical resistance or conductance of the component.
8. An apparatus as defined in claim 3, wherein said parameter is the current passing between the electrodes.
9. An apparatus as defined in claim 1 wherein the distance between the sensing electrode and the outlet end is sufficiently small so that as the component flows out of the separation capillary, the component comes in substantially continuous contact with the sensing electrode.
10. An apparatus as defined in claim 9, wherein the reference electrode and the sensing electrode form an electrode pair for detecting the electrochemical reactions at the sensing electrode.
11. An apparatus as defined in claim 1, wherein the reference electrode and the sensing electrode form an electrode pair for detecting the electrochemical reactions at the sensing electrode.
12. An electrical detection method useful in capillary electrophoresis comprising the steps of:
positioning the outlet end of a separation capillary into a buffer reservoir;
positioning a sensing electrode at a sufficiently small distance outside the outlet end of the separation capillary so that as a separated component flows out of the separation capillary, the component is not substantially diluted by the buffer;
placing a grounding electrode in the buffer;
establishing a current between the sensing electrode and grounding electrode;
establishing an eluent gap through which the component flows; and
measuring the change in electrical resistance or conductance between the electrodes.
13. The method of claim 11, further comprising applying an electrical potential between two points along the separation capillary, one point being the outlet end, for electrophoretic separation.
14. An electrochemical detection method useful in capillary electrophoresis comprising the steps of:
positioning the outlet end of a separation capillary into a buffer reservoir;
positioning a sensing electrode at a sufficiently small distance outside the outlet end so that as a separated component flows out of the separation capillary, the component comes in substantially continuous contact with the sensing electrode;
placing a reference electrode in the buffer;
applying a current between the sensing electrode and reference electrode; and
measuring the change in current between the electrodes in response to the component.
15. The method of claim 14, further comprising applying an electrical potential between two points along the separation capillary, one point being the outlet end, for electrophoretic separation.
16. The apparatus of claim 1, further comprising means for applying electrical potential along the capillary between first and second points for causing electrophoretic separation, wherein the first point is at the outlet end.
17. The apparatus of claim 16, wherein said electrical potential applying means includes:
a first electrode electrically coupled to the second point of the capillary; and
a second electrode electrically coupled to the outlet end.
18. The apparatus of claim 17, wherein the second electrode is also the reference electrode, said reference electrode being substantially at ground potential with respect to the sensing electrode.
19. The apparatus of claim 17, wherein the second electrode is different from the reference electrode.
The government has rights in this invention, pursuant to NSF Contract No. CHE-8657193.
The invention relates generally to capillary electrokinetic devices and in particular to an improved system for detecting electrokinetically separated species with electrical or electrochemical measurement.
Zone electrophoresis in capillaries has become an important technique in the repertoire of liquid-phase separations. See Jorgenson et al., Science 222 (1983) 266-272; Gordon et al., Science 242 (1988), 224-228; Ewing et al., Anal Chem. 61 (1989), 292A-303A; Wallingford et al., Advances in Chromatography 29 (1989), 1-76; and Kuhr, Anal. Chem., 62 (1990), 403R-414R. Capillary electrophoresis has been used for separations of small and large molecules and comprises several subtechniques including capillary zone electrophoresis (CZE), capillary gel electrophoresis, micellar electrokinetic capillary electrophoresis, and capillary isoelectric focusing. CZE employs extremely high potential fields, typically 300 V/cm, resulting in highly efficient separations of ionic solutes.
A major aspect of CZE in need of new development is detection; specifically, there is a critical need for detectors capable of responding to the small quantity of sample component in the effective detection volume. Detection schemes developed to date include direct and indirect UV absorption (Hjerten, J. Chromatogr., 347 (1985) 191-198 and Hjerten et al. J. Chromatogr., 403 (1987), 47-61), fluorescence (Jorgenson et al., Anal. Chem., 53 (1981), 1298-1302 and Kuhr et al., Anal. Chem., 60 (1988), 2642-2644)), and radioisotope (Pentoney et al., Anal. Chem., 61 (1989), 1642-1647) as well as mass spectrometric (Smith et al., Anal. Chem., 60 (1988), 436-441; Lee et al., Biomed. Environ. Mass Spectrom., 18 (1989), 844-850; Moseley et al., Chromatogr., 480 (1989), 197-210; Caprioli et al., J. Chromatogr., 480 (1989), 247-258)), and electrometric (Mikkers et al., J. Chromatogr., 169 (1979), 11- 20; Huang et al., Anal. Chem., 59 (1987), 2747-2749; and Wallingford et al., Anal. Chem., 59 (1987), 1762-1766)) detectors.
Electrical detectors can be distinguished from electrochemical detectors. Electrochemical detectors involve electric effects due to chemical changes which occur when a particle or species enters the detection zone. Electrical detectors respond to changes in the conductance of current or changes of resistance which result when particles or species enter the detection zone. With electrical detectors no chemical reaction is necessarily associated or required. Existing electrical and electrochemical detectors for CZE use elaborate on-column and post-column detection schemes to prevent the high separation potentials used from interfering with the detection process. One scheme involves construction of 40-μm-diameter holes in the capillary using a laser. Thereafter, small platinum wire electrodes are placed in these holes to carry out on-column conductivity detection. It was demonstrated that the exact placement of these electrodes on opposite sides of the capillary is critical to minimize background noise associated with the high potential field used for separation. (Huang et al., Anal. Chem., 59 (1987), 2747-2749). In U.S. patent. application Ser. No. 443,059, filed Nov. 28, 1989, by Zare et al., on-column conductivity detectors were disclosed wherein on-column sensing electrodes are located contiguous with the exit of the separation microcolumn. The electrodes are covered on their side surfaces with electrical insulators so that conductivity is measured between their ends alone. Another scheme involves covering a crack in the capillary with a porous glass capillary (Wallingford et al., Anal. Chem., 59 (1987), 1762-1766)) to provide off-column amperometric detection.
Although good results have been obtained with current detection systems, they are unsatisfactory in that the structures involved are difficult to fabricate, expensive and, often times, unreliable. This has limited the routine application of both modes of electrochemical detection in CZE. See Ewing et al., Anal. Chem. 61 (1989), 292A-303A; Kuhr, Anal. Chem., 62 (1990), 403R-414R; Huang et al., Anal. Chem., 61 (1989), 766-770; Huang et al., J. Chromatogr., 425 (1988), 385-390; Huang et al., J. Chromatogr., 480 (1989), 285-288; Wallingford et al., Anal. Chem., 60 (1988), 1972-1975; Wallingford et al., Anal. Chem., 60 (1988), 258-263; Wallingford et al., J. Chromatogr., 441 (1988), 299-309; and Wallingford et al., Anal Chem., 61 (1989) 98-100.
It is an object of the present invention to provide reliable and relatively inexpensive off-column electrical and electrochemical detectors capable of responding to the small quantity of sample components in the effective detection volume in CZE.
It is another object of the present invention to provide end-column conductivity and amperometric detectors that eliminate detection problems associated with the high voltage drops across the capillary in CZE separations.
These and other objects are achieved by the present invention which provides a new design for CZE conductimetric and amperometric detectors in which a sensing microelectrode is placed at the outlet of the separation capillary. These "end-column detectors" are easy to construct. They do not suffer from electrical interference caused by the applied high voltage during the CZE separation. Moreover, end-column detectors demonstrate sensitivities approaching those of previous on-column conductivity and post-column amperometric detectors (Ewing et al., Anal. Chem. 61 (1989), 292A-303A; Huang et al., Anal. Chem., 59 (1987), 2747-2749; and Huang et al., Anal. Chem., 61 (1989), 766-770) with only a small sacrifice in resolution. Under typical operating conditions the use of an end-column CZE conductivity detector is found to cause extra zone broadening only about 25% larger than that associated with an on-column CZE conductivity detector. End-column amperometry provides detection limits as low as 56 amol with an efficiency of 143,000 theoretical plates for catechol.
FIG. 1 is a schematic drawing of a CZE separation device with an end-column conductivity detector.
FIG. 2 is a cross-sectional view of the plastic jacket assembly.
FIG. 3 is an enlarged view of the end-column sensing microelectrode.
FIG. 4 is a schematic drawing of a CZE separation device with an end-column amperometric detector.
FIG. 5 is a schematic drawing of an end-column amperometric detector.
FIG. 6 is an electropherogram obtained with an end-column conductivity detector.
FIG. 7 is an electropherogram obtained with end-column amperometric detection.
FIG. 8 is an electropherogram obtained with end-column amperometric detection.
FIG. 1 shows a CZE separation device with an end-column conductivity detector. The separation capillary 100 is a 80 μm i.d., 354 o.d. fused silica microcolumn with a length of 60 cm (Polymicro Technologies, Phoenix, Ariz.). A reversible high voltage power supply 110 (Model R50B, Hipotronics, Inc., Brewster, N.Y.) provides a variable voltage of 0-30 kV with the outlet of the separation capillary at ground potential.
The capillary is liquid-filled with a support electrolyte and terminates near a sensing microelectrode inside plastic jacket 200. The jacket is approximately 25 mm long and has a diameter of approximately 6.4 mm. Inlet reservoir 120 and outlet reservoir 130 contain support electrolyte as well, so that the liquid-filled capillary 100 creates a continuous liquid and electrical connection between them. As will be further described below, the electrolyte exiting the capillary flows into the outlet reservoir by way of a hole 290 in the plastic jacket 200. The jacket is inserted part-way into the outlet reservoir through one of the reservoir walls and is held in position thereby. An effective electrokinetic voltage is supplied from power supply 110 through conductors 140 and 150 and grounding electrode 170 and electrode 160. A sensing microelectrode (described below) is situated at the outlet of the capillary 100 inside plastic jacket 200. The output of the end-column conductivity detector which varies as a function of the material in the electrolyte, is passed through lead 270 to conductivity meter 180, and is recorded. The conductivity measurement is made between the sensing microelectrode and the grounding electrode 170.
FIG. 2 is a cross-sectional view of the plastic jacket assembly. Lead 270 is positioned inside the 1 cm long fused silica capillary 220 (150 μm i.d. and 355 μm o.d., Polymicro Technologies) that extends midway into the plastic jacket. Similarly, separation capillary 100 extends midway into the plastic jacket from the other direction. Epoxy 210 and Teflon� washer 240 provide support for the capillary 220. Similarly, epoxy 250 and Teflon� washer 260 provide support for separation capillary 100. Around its middle section, the plastic jacket defines channel 280 into which capillary 220 and separation capillary 100 are positioned. The channel is sealed at its ends by the Teflon� washers 240 and 260. A hole 290 on one side of the plastic jacket lines up with the sensing microelectrode so that fluid flows off the separation capillary 100 and into the reservoir 130.
FIG. 3 is an enlarged view of the sensing microelectrode 300, which is made of a 50 μm diameter platinum wire (California Fine Wire Co., Grover City, Calif.). The microelectrode is centered in capillary 220, and held in place by epoxy 310 (Torrseal, Varian Corp., Lexington, Mass.). The surface of the microelectrode facing the outlet of separation capillary 100 is sanded flat.
The end of capillary 220 facing the separation capillary is positioned inside a short but somewhat larger support fused silica capillary 320 (approximately 355 μm i.d.), which resembles a ring or collar structure. The outer surface at the end of capillary 220 is sealed with epoxy to the inner surface of one end of capillary 320. The other end of capillary 320 extends about 1-2 mm over the outlet end of the separation capillary 100, which is nearly butted against the sensing microelectrode 300. This leaves a small path or eluent gap 330, about 1-2 mm long, with a wall separation between separation capillary 100 and capillary 220 of approximately 1-5 μm, and preferably of approximately 1-2 μm, for the eluent to exit into the outlet reservoir 130 via hole 290. The eluent gap can be defined generally as a narrow channel that serves as the detection zone for electrical detectors. The dimensions of the eluent gap depend on, among other things, the amount of sample injected into the separation capillary for analysis. With the inventive conductivity detector, the resistance of each component zone or band is measured as each band flows through the eluent gap. If the gap length is too long, more than one band will be present in the eluent gap at a given time thereby interfering with the measurement. Increasing the wall separation distance reduces the gap length, but has the concomitant adverse effect of creating additional dead volume.
FIG. 4 is a schematic diagram of the CZE system used for amperometric measurements. The separation capillary 400 is a fused-silica capillary 5 μm i.d./140 μm o.d., Polymicro Technologies (Phoenix, Ariz.). The capillary (50-70 cm in length) is positioned in plastic vessel 410 through a bore defined by stainless steel fitting 420 (shown with size exaggerated) that is epoxied to one side of the vessel. The vessel functions as the electrochemical cell. The fitting also serves as the cathode for electrophoresis. The high voltage DC power supply 430 provides the electric field for electrophoresis. Electrode 440 situated in buffer reservoir 470 is connected to the power source by conductor 450. Similarly, stell fitting 420 is connected to the power source by conductor 460. The end of the microelectrode 530 (described below) of the detector 480 is manipulated through a slot cut into the opostie side of plastic vessel 410 and up against the end of the separtion capillary 400 with a micromanipulator (Newport, Model 422) while viewed under a microscope.
FIG. 5 is a schematic top view ofthe ampoteric detector. In constructing the detector, a single carbon fiber, 10 μm diameter, Amoco Performance Products, Greenville, S.C., is aspirated into glass capillary 500. The capillary was pulled around the fiber 530 with a vertical microelectrode puller. The fiber 530 serves as the microelectrode. Under an optical microscope, a drop of epoxy 540 is applied to the area where the fiber entered the glass capillary. After curing, the fiber is cut with a scalpel to an exposed length of 0.1-1 mm. The open end of the glas capillary is filled with mercury, a segment of nichrome wire 590 placed into it, and sealed with a drop of DUCO cement.
The carbon fiber detector is cemented onto a microscope slide 510 so that the end containing the exposed fiber protruded from the edge of the slide. The entire detector assembly is then placed onto a micromanipulator 520, Oriel Cor., Stratford, Conn. The tip of the carbon fiber is aligned with the bore of the separation capillary 400. The separation between the tip of the carbon fiber and the separation capillary is approximately 1-5 μm. The carbon fiber electrode 530 and outlet of the separation capillary 400 are submerged in the buffer solution of electrochemical cell 410 wherein a reference electrode 550 is also positioned. Reference electrode 550 is conencted to potentiostat 570 by lead 560; nichrome wire 590 is connected to the potentiostat by lead 580.
Conductivity Detection. For conductivity measurements, the end-column conductivity detector was placed directly at the outlet of the CZE capillary (as shown in FIG. 3). Samples were introduced by gravity at the cathodic or the anodic end of the capillary by raising the inlet a known height (7-12 cm) with respect to the outlet for a fixed period of time (5-10 s).
Amperometric Detection. For amperometric measurements, the cell was filled with 0.1M KCl as supporting electrolyte. Detection was performed in a 2-electrode configuration with a sodium saturated calomel reference electrode (SSCE). Electrochemical detection was carried out at 0.8 V SSCE. The low currents measured required that the detection end of the system be housed in a Faraday cage in order to minimize the effects of external noise sources. Injection was by electromigration
Chemicals. All chemicals were from Sigma Chemical Corp. (St. Louis, Mo.) and were used without further purification. Conductivity detection runs were carried out with a buffer solution consisting of 20 mM morpholinoethanesulfonic acid (MES)/histidine at pH 6.0 to which a cationic surfactant, tetradecyltrimethylammonium bromide (TTAB), is added (1 mM final concentration). Water used to prepare solutions is freshly deionized and distilled with a water purifier (Model LD-2A coupled with a Mega-Pure Automatic Distiller, Corning Glassworks, N.Y.).
Amperometric detection runs were also carried out with MES, but these buffers were adjusted to the desired pH by addition of solid NaOH. Catecholamine stock solutions were prepared as 0.01 M solutions in 0.1M perchloric acid and diluted to the desired concentration with operating buffer.
End-Column Conductivity Detection. FIG. 6 shows an electropherogram obtained when a mixture containing six different carboxylic acids at 5�10-5 M each is injected onto the CZE setup illustrated in FIGS. 1-3. The acids were: formate (peak 1), acetate (peak 2), propanoate (peak 3), butanoate (peak 4), pentanoate (peak 5), and hexanoate (peak 6). The CZE is operated at 20 kV and 8.8 μA. The buffer is MES/HIS (20 mM each) at pH 6 with 1 mM TTAB. Injection was by gravity.
The sensitivity and the resolution are quite similar to what has been observed previously (see FIG. 1 of Huang et al., Anal. Chem., 61 (1989), 766-770). Because the TTAB in the buffer reverses the electroosmotic flow direction, the polarity of the power supply was also reversed so that the most mobile anion arrives first at the detector.
This end-column conductivity detector has more dead volume than an on-column conductivity detector. This additional dead volume estimated to be about 5-6 nL arises from the eluent gap 330 between capillaries 100, 220, and 320 as shown in FIG. 3. To investigate the effect of this dead volume on the separation efficiency, the following experiment was carried out. Benzoate anion (C6 H5 COO-) was injected at 1�10-5 M and the peak profile was recorded using on-column UV absorption and end-column conductivity detection. The UV absorption detector was 9 cm upstream from the end-column conductivity detector, so that the additional zone broadening in traveling this distance is minimal (Huang et al., J. Chromatogr., 480 (1984), 95-110). It was found that the peak from the end-column conductivity detector is 23% broader than that for the UV absorption detector, at an injection volume of 23 nL. This extra zone broadening caused by the additional dead volume of the end-column conductivity detector compares well with the estimated magnitude of the extra broadening.
For typical injection volumes (20 nL), and for a 80-μm-i.d. capillary, this extra zone broadening is less than 25%, which is modest compared to the advantage of such a simple construction. Moreover, the end-column conductivity detector can be readily fitted on the outlet of any capillary electrophoresis system, suggesting its use as a simple universal second detector for CZE separations. Furthermore, CZE employing the inventive conductivity detectors can yield quantitative measurements on an absolute basis when an internal standard is used.
End-Column Amperometric Detection. As described above, the CZE system for amperometric detection comprises essentially a fused-silica capillary and a carbon fiber microelectrode. With the inventive end-column amperometric detector, there is no need to isolate the sensing electrode from the high electric field needed for electrophoresis because the voltage drop outside the bore of the separation capillary is negligible. The microelectrode is aligned with the bore of the capillary and positioned up against but not into the capillary, thereby creating a thin layer cell at the capillary outlet. Because the diameter of the microelectrode in this embodiment is about twice the internal diameter of the capillary, good oxidation efficiency is obtained. Detector sensitivity is further increased by approximately one order of magnitude when a cylindrical carbon fiber electrode is used, as shown in FIG. 5, relative to a disk-shaped electrode. Apparently the eluent from the capillary forms a sheath-like flow around the electrode, providing more efficient oxidation of solutes at the detector. The separation conditions for the electropherograms in FIGS. 7 and 8 were: separation potential, 20 kV; electrochemical detector potential, 0.7 V; injection, 20 kV for 5 s.
The electropherogram in FIG. 7 shows the separation of equimolar (1�10-5 M) concentrations of dopamine (peak 7), isoproterenol (peak 8), catechol (peak 9), and 3,4-dihydroxyphenylacetic acid (peak 10) obtained with a 5 μm i.d. capillary that was 56.6 cm long. It is clear that cations, neutrals, and anions are readily separated using this technique.
FIG. 8 shows an electropherogram obtained in a separate study of the linearity of the detection system. An equimolar (5�10-6) mixture of dopamine (peak 11), isoproterenol (peak 12), and catechol (peak 13) was injected onto a 5 μm i.d., 56.6 cm long capillary. The peaks correspond to 960 amol dopamine, 870 amol isoproterenol, and 560 amol catechol. No attempt was made to optimize these separations.
Standard calibration curves for test solutes were computed. In one set of experiments, detection of catechol was examined for total injection amounts ranging from 1.13 fmol to 0.113 pmol (10-5 to 10-3 M). Linear regression analysis provided values for the slope, and correlation coefficient of 7.7�10-3 and 0.991, respectively.
In another set of experiments, detection of dopamine, isoproterenol, and catechol was examined for average total injection amounts ranging from 80 amol to 4.0 fmol (5.0�10 to 2.5�10-5 M). Linear regression analysis provided correlation coefficients of 0.998 for DA, 0.999 for IP, and 0.993 for CAT. The detection limits computed for these compounds are 64 amol for DA, 70 amol for IP, and 56 amol for CAT (S/N=2, peak-to-peak noise evaluated over 10 peak widths). These values were calculated based on the lowest amount injected, which was 5�10-7 M (80 amol).
It is to be understood that while the invention has been described above in conjunction with preferred specific embodiments, the description and examples are intended to illustrate and not limit the scope of the invention, which is defined by the scope of the appended claims.
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Albuquerque, N.M. Julia Phillips, director of Nuclear Weapons Science and Technology Programs at Sandia National Laboratories, and Sandia researcher Neville Moody have been named Fellows in the Materials Research Society (MRS).
The title of Fellow honors MRS members for their research accomplishments and contributions to materials research worldwide. The maximum number of new Fellow appointments each year is limited to 0.2 percent of the society's current membership. Twenty-nine Fellows were named this year.
Phillips was honored "in recognition of research accomplishments in thin-film heteroepitaxy, national/international leadership in materials science and contributions to help shape the MRS organization," in a paragraph released by the Society.
Moody was similarly mentioned "for outstanding research characterizing the deformation and fracture of materials, for service shaping the quality of professional society activities, and for mentoring generations of students, professors and researchers."
Phillips joined Sandia as a manager of a materials science organization in 1995. Between 2001 and early 2010 she served as director of Sandia's Physical, Chemical and Nano Sciences Center. The center, with an annual budget of nearly $50 million, performs fundamental research and technology development supporting the breadth of Sandia's national security mission in nanoscience and nanotechnology, compound semiconductors, radiation effects and remote sensing. Areas of particular emphasis that emerged during her tenure include the science and technology of solid-state lighting (including a DOE Energy Frontier Research Center focused on inorganic solid-state lighting), nanoscience (including the DOE Center for Integrated Nanotechnologies), and quantum computing. Her concurrent duties at Sandia included overall leadership of the Laboratory's Office of Science program (nearly $90 million in FY2009), service on the board of directors for th
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Hey Everyone! It's Friday and I'm excited for the weekend! It's looking like it will be a beautiful one too, although a little on the chilly side. It's supposed to be in the mid 50s to mid 60s which is not necessarily that warm but it'll be sunny. For the "in between" temperatures we get(…)
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Rihanna Drops $12 Million On Pacific Palisades Mansion
December 18, 2012 10:37 AM
Rihanna is the proud owner of an 11,000 square-foot mansion in the Pacific Palisades.
The 24-year-old "Umbrella" songstress, whose been promoting her 7th studio album Unapologetic, bought the gorgeous pad for $12 million.
RiRi's digs boasts 7 bedrooms, 9 bathrooms, top range kitchen, dining room with a 14-foot ceiling, media rooms, outdoor BBQ and bar area, swimming pool, a 6,000 square-foot back garden, a sun deck, 4-car garage, a hot tub and stunning panoramic views of the city.
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South Korea rescue helicopter and fishing boats try to rescue passengers from a passenger ship in water off the southern coast in South Korea, Wednesday, April 16, 2014. The South Korean passenger ship carrying more than 470 people, including many high school students, is sinking off the country's southern coast Wednesday after sending a distress call, officials said. There are no immediate reports of causalities. (AP Photo/Yonhap) KOREA OUT
Photo by Associated Press
Photo by Associated Press
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Original article: Fears rise for missing in SKorea ferry sinking
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A solemn crowd gathered to honor 43 people who lost their lives a year ago in the deadliest landslide in U.S. history.
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Take a look at the best photos of the week from around the world.
Tuesday in photos
St. Patrick's Day, March 17, 2015.
Monday in photos
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Week in photos
Photos from around the world this week.
Friday in photos
The second of three Friday the 13ths in 2015; Friday, March 13, 2015.
Thursday in photos
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I'm a musician, writer, comedian, collage artist, pod caster, information junky. I like to have as much fun as I can doing things that further my path. I like to drink and talk about funny but intelligent things. I like to read silly fiction or super serious nonfiction. I'm a sucker for conspiracy theories, too. I obsessively research things like the band Queen or sci fi writers perhaps.
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My self-summary
Write a little about yourself. Just a paragraph will do.
What I'm doing with my life
Don't overthink this one; tell us what you're doing day-to-day.
Crazy podcast, writing a sci fi novel, making collages for friends
I always have a million projects going. Hopefully I'll see a completion before I'm dead.
I always have a million projects going. Hopefully I'll see a completion before I'm dead.
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Go on, brag a little (or a lot). We won't judge.
Making people laugh. Keeping things out of the ordinary (in a good way). Providing warmth for my friends. Making people think.
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That I look pissed off. Well, that I'm witty, too.
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Favorite authors are Harvey Pekar, Charles Bukowski, Richard Braughtigan, Hunter S Thompson. Favorite artists are Robert Crumb, Charles Burns, Jackson Pollock. I like biker movies, situational comedies like The Graduate, Man on the Moon. Brazil is one of my favorites, too. I've been watching a lot of documentaries lately. I like cerebral electronic music, dream pop, synth pop, The Jesus and Mary Chain, Radiohead, Electric Wizard. I like to eat at quirky diners as far as food goes. Clockwork Orange. Spiritualized. Buffalo 66 is my favorite movie.
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Think outside the box. Sometimes the little things can say a lot.
Stimulation, alleviation with laughter, my cat, Christmas lights in the bathroom, movies, snap shirts.
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Global warming, lunch, or your next vacation… it's all fair game.
How to improve myself, why people do the things they do, how to relax my brain, behind the scenes of anything
How obnoxious it is that my okc personality is really 'drug friendly'. I rarely do drugs anymore. Why would they put that on a profile, Jesus! I might as well write on here that I'm a drug addict. The dates will pour in then. I'm sure.
How obnoxious it is that my okc personality is really 'drug friendly'. I rarely do drugs anymore. Why would they put that on a profile, Jesus! I might as well write on here that I'm a drug addict. The dates will pour in then. I'm sure.
On a typical Friday night I am
Netflix and takeout, or getting your party on — how do you let loose?
Having a movie night with friends, drinking, looking up whatever subject is stuck in my head, past life regression and meditation (for real)
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I can be kinda shy and I like to look up classic wrestling matches on youtube.
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Norton Commons is a New Urbanist development that straddles the Jefferson/Oldham county line.
Forget Birnam Wood coming to Dunsinane—come to Inverness
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"The Christmas Wish," a picture book by a Minneapolis couple, is flying off the shelves – much as photo studio magic has their daughter, Anja, flying with reindeer.
Updated: December 24, 2013, - 11:22 AM
Poet Joyce Sidman, with a new national honor, explores in her latest book how words can cast a spell, and why we should bestow blessings on things that make us glad.
Updated: December 17, 2013, - 02:57 PM
From iconic recipes to innovative ideas, these half-dozen baking books offer a range of choices for the bakers on your...
Updated: December 11, 2013, - 03:32 PM
Art and function come together in the "Sota" cast-iron skillet created by Milwaukee artist Alisa Toninato at her FeLion Studios.
Updated: December 11, 2013, - 03:23 PM
"Peephole driving" is the death-defying belief that peering through a Frisbee-sized porthole in your windshield enables you to see well enough to drive in winter.
Updated: December 10, 2013, - 08:29 AM
A glimpse into our universe, and many others, is inspiring, humbling, chilly – and a privilege.
Updated: November 30, 2013, - 03:49 PM
When it comes to scholarship applications, you have one chance to present your best self. Some tips from one who...
Updated: November 26, 2013, - 01:42 PM
For 35 years, Bruce Bjerva has provided the lines within which thousands of kids have flexed their creative muscles.
Updated: November 25, 2013, - 03:13 PM
The Minneapolis Kennel Club Dog Show will celebrate its 80th year and its future – in the form of kids and their pups.
Updated: November 19, 2013, - 08:11 AM
Leftover Thanksgiving turkey needn't be an afterthought. In a creamy pot pie, bolstered with root vegetables, it might actually taste better than the original feast.
Updated: November 13, 2013, - 02:29 PM


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I believe this product is only available as a Public Beta for which I am a tester. Despite my very limited use of beta-products, the OS X betas after the retail release have been very stable. I have only had 2-3 issues for which I filed RADARs on and those issues were fixed in a […]
Following a short beta testing period, Apple's iCloud.com website has been updated to make its iWork suite of apps accessible to all users, even those without an Apple device. As of today, anyone can sign up for an Apple ID to access Pages, Numbers, and Keynote for free. Show More Summary
A couple of weeks ago, we noticed Apple's beta iCloud site was allowing anyone to sign up for the company's productivity suite on the Web – no Apple device required. If you tried to sign up via the standard iCloud site, however, you'd be presented with an error. Show More Summary
Apple is now letting anyone create a free Apple ID on the public iCloud website and use Pages, Keynote, and Numbers for free. This comes just two weeks after the initiative was rolled out on the iCloud beta website. To get started, users can visit iCloud.com and create an Apple ID. Show More Summary
Apple on Thursday removed the beta tag from its Apple ID creation tool, granting anyone, on any platform access to access to the iWork for iCloud Web-based productivity suite.
Apple moved its web-based versions of its iWork office suite, including Pages, Numbers, and Keynote out of iCloud's beta site and onto iCloud.com on Thursday. The web versions are free, and…
Apple has added the web-based iWork, which works for anyone with an Apple ID for free on most modern browsers, to the main iCloud.com website. The previously beta-only iCloud offering makes Apple's productivity suite accessible to anyone...Show More Summary
Apple has opened up the web-based version of iWork to everyone—even if you don't own an Apple device. Sign up through the iCloud beta [via TechCrunch] Read more...
If you've ever been curious about Apple's alternative to Office Online and Google Docs, but don't own an Apple product, you may be in luck. The company is now allowing anyone to sign up for a beta version of iWork for iCloud – no Apple device required. Show More Summary
If you've been interested in trying out Apple's iWork suite of productivity apps for yourself, but don't have an Apple device to try them on, great news: anyone can now create an Apple ID and sign into the iCloud Beta … Read more ›
Add another option to the freely available online productivity software suites: Anyone can sign up for and use Apple's iWork for iCloud, even if they don't have a Mac or iOS…
Apple has opened up its iWork for iCloud beta, allowing any user to create a new Apple ID and use the service, regardless of whether they are an iOS or Mac user or not. Although the feature is only available on the beta iCloud site,Show More Summary
Apple has taken another step toward turning iWork into a service anyone can take advantage of, similar to Google's web-based productivity suite. The company made its iWork for iCloud apps available to anyone, regardless of whether or not they have any Apple hardware, so long as they have or sign up for an Apple ID. Show More Summary
Apple on Thursday made Pages, Numbers and Keynote accessible to users without an Apple device through iCloud Beta. Starting last night, anyone can sign up for an Apple ID to access the trio of iWork for iCloud beta apps for free. Each...Show More Summary
Good news for people wanting to use Apple's productivity software: starting tonight, anyone, with or without a Mac or iOS device, will be able to create an Apple ID and sign in to the iCloud beta website to start using Pages, Numbers, and Keynote for free. Show More Summary
Our long national iCloud photo-syncing nightmare is almost over: Apple's put out some new information about Photos for Mac, OS X Yosemite's official successor to iPhoto, and released an early private beta inside OS X 10.10.3 for developers. Show More Summary
Apple's first beta of the new Photos application for OS X showcases a sleek, uncluttered interface that will instantly feel natural to anyone who has used the modern Photos app for iPhone and iPad, bringing welcome features and strong iCloud integration to the desktop.
Apple is rolling out the first pre-release seed of OS X 10.10.3 to testers and developers today including the all-new Photos app with support for iCloud Photo Library beta. The new Photos app replaces iPhoto while Aperture is no longer being developed either. We'll update coverage with our hands-on with the new release.Filed under: AAPL […]
Apple today released OS X Yosemite 10.10.2, marking the second update the operating system has received since its October release. Testing for OS X 10.10.2 began in late November, with Apple seeding six betas to developers before releasing...Show More Summary


text:
directed by Christine Jeffs. UK, 2003, 110 min, original version with Italian subtitles
Sylvia Plath, one of America's greatest contemporary writers and the wife of celebrated poet Ted Hughes, devoted her genius to translating an awareness of the pain of living into disturbing prose, right up to her tragic suicide at the age of only thirty-one.
To tie in with the Matisse. Arabesque exhibition
18 March > 13 May 2015
Documentaries on Matisse. To tie in with the Matisse. Arabesque exhibition
19 > 29 March 2015
notes of Cinema History
28 October 2014 > 19 May 2015


text:
- Meet John Doe: Businesses, politicians and gov't should follow junk email laws
- Entertainment abound for this week's First Friday
- State Roundup: Special election dates set
- Test drive: the 2015 Ford F-150
- Fracking never on a path to sustainability
- Indiana boxes itself into legal corner
- TRRT April 1-7 | Online Edition
- Guest Commentary: the Rockford Apartment Association
- State Roundup: NIU employee improperly reimbursed $30K
- State Roundup: Governor signs budget fix bills
Community news and notes: week of Jan. 13-19, 2010
Ryan Spiess, of Rockford, joined Century 21 Country North as a sales associate. He will specialize in residential property sales in the Rockford/Winnebago area. Veronica Ortega returned as a sales associate to the office. Investment Realtor Rick Davis and his licensed assistant, Dawn James, also joined the Rockford office. … Beloit Regional Hospice awarded the 2009 Spirit of Caring Award to Kim Beals, the owner of Brill Jewelers in Beloit, Wis. Beals has donated a variety of items for the diamond giveaway for each of Beloit Regional Hospice's signature event, Doves and Diamonds: Celebrate Hospice. His commitment to the organization has also touched many through his advocacy, donations of jewelry to support other fund-raising efforts for the organization, and being interviewed for "A Hospice Moment" radio program aired on WGEZ in 2009. The Spirit of Caring Award is given annually to an individual, organization or business that has demonstrated dedication to strengthening the hospice concept in the stateline community in an effort to improve quality of life. … Asad Jamal, M.D., recently joined OSF Medical Group and has begun seeing new patients at the new OSF Medical Group-Cherry Valley. Dr. Jamal is a family medicine physician and earned his medical degree at Kazakh State Medical University in India. He completed his residency at Loyola/Cook County/ Provident Family Practice in Chicago. Dr. Jamal most recently worked at Fayette County Hospital and Long-Term Care in central Illinois.
Send your "Community news and notes" to The Rock River Times, ATTN: People In Our Times, 128 N. Church St., Rockford, IL 61101; e-mail firstname.lastname@example.com; call (815) 964-9767; or fax (815) 964-9825.
From the Jan. 13-19, 2010 issue.


text:
Calif. considers a plea for plug-in hybrids
Valeo's self-driving systems learn from military drones
Buick Enclave upgrade gets Tuscan treatment
Leases to get more expensive, ALG says
AutoNation, analytics company Zubie join to monitor cars' service needs
Dealer groups charge CFPB is unresponsive
UAW troops air demands at convention rather than cast blame
With Volvo's help, Geely goes upscale
If dealers don't weigh in, they can't gripe
AutoNews Now: Rewards of being Ford CEO
First Shift: Mercedes planning a pickup
Scion iA spy photos
April 7 at 2pm ET: Tap Into Text With Auto Shoppers and Make More Connections
April 14 at 2pm ET: The Road to Your Growth: Why U.S. Hispanic Auto Consumers are a Must-Win Target
April 22 at 2pm ET: Is Your F&I Experience Being Left Behind?
2015 Automotive News PACE Awards
2015 Automotive News Marketing Seminar - Los Angeles
Automotive News Europe Rising Stars 2015
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General Motors spent $131 million to retool a plant in Bowling Green, Ky., to assemble the redesigned 2014 Chevrolet Corvette Stingray, which features a new frame, body and suspension.
Workers inspect the 2014 Chevrolet Corvette Stingray at GM's assembly plant in Bowling Green, Ky.


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free (new!) EP on Soundcloud
gig, chatroom, radio (twice) – a good night
Normally I hate and fear February, but this one's already shaping up to be an exceptionally good month. All in one night, did a gig:
Then had In The Evil Empire played on MuseBoat Radio (on TJ's Muse Bridge… old-fashioned, but I still get a kick out of hearing my stuff introduced by an American voice)… and went into the site chatroom during the programme, which was an interesting new experience – and fun, if nail-biting… and then found out the track was also being played on Source FM the same night, on a prog called Random Jukebox.
Phew. Actually this was last night, so am still a little jangled. Hope this post made sense – if not, apply for a refund to the usual address. Buz buz. xx
Sunday night: live radio & chatroom action
So this is happening tomorrow night after the gig, thanks to Museboat Radio - I quote:
Join us on Sunday, Febr. 8th, 2015 in TJ´s Muse Bridge LISTEN & CHAT show introducing new artists and new songs to the MuseBoat Radio family at 10pm London ~ 11pm Berlin ~ 5pm New York ~ 2pm Las Vegas ~ 7pm Rio de Janeiro
You can login to the chatroom with your Facebook / Twitter / Google+ / Yahoo / LinkedIn or others without registration and promote your music amongst our listeners
Here is our show playlist
AMPIA VISTA – Nowhere To Run
ANNA ZED – Jump
Day Old Hate – Tides
Luis Drayton – Princess Despair
MARK S PROJECT – Dirty Little Mouth
Murky Red – Wild Flower
Paul Robert Thomas Song Lyricist – Just Us Two
RavenHeart – Wannabe Playboy
Technomancer FEAT ANGST POP – Electronic Warfare
The Open Feel – Retrofire
COLD SNAP – Carnival
EMMETT NORTH JR – Heart Of Stone
EMPIRE OF FOOLS – Devil Inside
FAREWELL DAVIDSON – Fixate
IVEZA AND ELEMENTS – Ignore the sign
JOE FERRERI – Sometimes
MATT FINUCANE – In The Evil Empire
NORDIC´S YELL – Nocturnal Frenzy
OZEYLAA – Rainfall
SOUL HOWE – Between the lines
playing live Sun 8 Feb – come, of yr own free will…
Looks fun. Free gig, promoted by the very talented Jacob Szulecki - and I say that not as a suck-up, but because I genuinely have no idea how he plays the stuff he does on guitar. Anyway, he's assembled some meaty talent for this event – come see & hear.
Also, I've got new songs to play! That's always a buzz – and if I get a buzz, you get a buzz. Reasonable, innit.
big in Japan?
Just heard that In The Evil Empire is scheduled for heavy rotation on a Japanese radio station.
That's Nagoya Radio - it's a bilingual site, and you'll be able to hear my single on it rather a lot, hopefully. Exciting stuff!


text: Find this wine Find this wine Valpolicella Ripasso 2008 Weich und kraeftig zugleich. Ganz ok... Pin It Tweet 3 years ago by Fbolte More posts Ripasso 2009 Valpolicella Classico Superiore 750 Ml Wine club winner 2 months ago by S C. Recchia Le Muraie Valpolicella Ripasso Bottle Great - rated 90/91 very smooth enjoyed with red sauce and pasta- Dec 2014 2 months ago by Tedjunk12 Rocca Sveva Ripasso Valpolicella Superiore 2009 Delicious 4 months ago by Mbarone N. Campolieti Valpolicella Ripasso Great valpolicella...Tuscan tavern n Plymouth ma $30...campolieti 6 months ago by Pauloncape Valpolicella Ripasso Zeni Aug. 23 2014 Annapolis, dinner at Maria's with the Mannings. 7 months ago by Pburtoft


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Archived Story
High-energy 'Footloose' on stage at M State in Fergus FallsPublished 11:26am Thursday, October 31, 2013 Updated 1:31pm Thursday, October 31, 2013
Time to kick off those Sunday shoes and cut back to the '80s with the "Footloose" musical put on by the M State-Fergus Falls theater and music departments.
Or you could listen to the advice of the director Stefanie Gerhardson.
"Get your flash dance on," she said.
"Footloose" will take audience members back to the 1980s, from the prom dresses to the music. It's a musical that hasn't been done at M State-Fergus Falls before, and it's one that Gerhardson always wanted to direct. It opens this weekend.
"It's just a fun musical that I know audiences like, and I know students like," she said. "It's a really high-energy, fun play."
The musical is very similar to the original "Footloose" movie from 1984 starring Kevin Bacon. It tells the story of a Chicago teenager who moves to a small town and challenges its ban on dancing and rock music, which were outlawed following a tragic accident.
"He kind of organizes the kids to repeal this law and bring dancing back," Gerhardson said.
Audience members will leave feeling good and entertained, Gerhardson said.
"It also has kind of a good message about tolerance and acceptance and how we process anger and grief," she said.
The musical is co-directed by Gerhardson and Teresa Ashworth, who have directed together for 20 years.
The cast features MyKenzey Watchorn, of Frazee, as the rebellious teen Ren McCormack, and Lexi Wedll, of Fergus Falls, as the girl he falls for, Ariel Moore. Cast members also include Spencer Imsande and Jackie Imsande, of New York Mills; Zach Anderson and Callie Thiel, of Wendell; Jacob Bauer, of Graceville; Sean Schneider, of Sauk Centre; Ben Walkup, Bradley Muckey and Kyle Rust, of Fergus Falls; Austin Begley, of Underwood; Eric Ronning, of Miltona; Josh Wilson, of Monticello; Amanda Collins, of Dent; Kristen Johnson, of Elbow Lake; Ashley Forsman, of Murdock; Rachel Moe, of Kensington; Samantha Bottko and Erin Bottko, of Battle Lake; and Keirsten Markgraf, of Pelican Rapids.
Performances will be at 7:30 p.m. Nov. 1, 2, 7, 8 and 9, and at 2 p.m. Nov. 3 in the Waage Theatre on the M State-Fergus Falls campus. Tickets are $12 in advance and $15 at the door for adults, and $7 in advance and $8 at the door for students. Tickets can be purchased at the M State Box Office, by calling 736-1600 or online at www.mstatetickets.com


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210A West 22nd Street
Huntington Station, New York, 11743
Commute to Downtown Huntington Station
Some errands can be accomplished on foot.
Score Details
210A West 22nd Street has a Walk Score of 55 out of 100. This location is Somewhat Walkable so some errands can be accomplished on foot.
This location is in Huntington Station. Nearby parks include Oakwood Park and Koster Park.
Nearby schools include Walt Whitman High School, Henry L Stimson Middle School and Oakwood Primary Center.
The closest grocery stores are King Kullen, N a Food Mart and Divine Brine Foods Inc..
Nearby coffee shops include Weekdays Cafe, Station Coffee Shop and Hungtinton Coffee Shop & Grocery. There are 14 restaurants within a 15 minute walk including BURGER KING®, McDonald's and Golden Coach Diner Restaurant.
Explore how far you can travel by car, bus, bike and foot from 210A West 22nd Street.
Rail lines:
Port Jefferson
1.3 mi
Bus lines:
n79 Hicksville - Walt Whitman Mall
0.3 mi
Car shares:
View all Huntington Station apartments on a map.
Popular apartment searches include pool, fireplace and hardwood floor. View all Huntington Station apartments and rentals.
This location is in the city of Huntington Station, NY. Huntington Station has an average Walk Score of 49 and has 33,029 residents.Learn More About Huntington Station


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The star--most famous for his role as a Los Angeles medical examiner in the long running series--exchanged nuptials with his partner of 20 years, Peggy Crosby, at the Little Brown Church in Studio City, California on Saturday.
Crosby is the ex-wife of Bing Crosby's son Philip. Klugman's first wife Brett Somers died last year. They married in 1953 and separated in 1974, but never divorced.
COPYRIGHT 2008 WORLD ENTERTAINMENT NEWS NETWORK LTD. All Global Rights Reserved.


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Even though we ARE Nikon lovers,we are NOT affiliated with Nikon Corp. in any way.
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I've found several GPS units that are compatible with my camera, but I'm not finding great information about which one is the best. They all claim they're the best. Can anyone recommend one to this newbie and tell me why?
No company will claims their products are worse than competitor I'm a D90 user and have same problems as you. D90 and D3100 have same ports so I assume the GPS units which can work with D3100 will work with my D90. I'm struggling between Solmeta geotagger Pro and foolgraphy bluetooth GPS. Solmeta Pro is the only one camera GPS I found with LCD,internal battery,electronic compass(provide heading information), memory card and has "indoor lock" functions, all of these features attract me. The price is under $250, I think it worths. The foolgraphy stands for its bluetooth, cableless looks cute and smarter, however it doesn't include all the functions that Solmeta has. So bluetooth or wired GPS, I need to take more time to make a decision.
You definitely sound like you're in the same boat as me. I don't so much care about the cost, but I only want to spend it once. I don't wanna drop $300 on one only to figure out that another one is what I SHOULD have gotten. Hate that!The foolgraphy does sound good.
I don't own one, but check out EasyTagger as well as the Solmeta. Jeez, this is about the third time I've posted on this....there should be a special section on the top of the forums to talk about GPS units.
$250 is not a small money. And what is the use of lcd screen? While taking photos, I want all my camera gadgets as simple as it can be. What is the best? In my understanding, is the lock-on time and accuracy and also be simple, saving me all my effort to concentrate on capturing the beauty of the nature.I read a lot good comments on Easytagger and Solmeta, and also Dawntech and Phottix, so my understanding is they are all good, just choose the most cost effective one.
What do you mean as best?Longest battery life?Fastest satellite acquisition?Most accurate?Most features?Note that accuracy is controlled by the controller of the GPS satellites, usually a country's military service. Weather and terrain can also affect the accuracy. Steel buildings, granite mountains, and dense vegetation can also affect the radio signals from the satellites.Almost all have the same internal circuitry.Many handheld GPS units track more data than the camera specific units.Do you want your GPS to do more then tag your images?I use the Garmin GPSMap 60CSx for land and water navigation as well as for tagging images.You might also want to read the manual for the GPS you are interested in and see what data it collects and what data your camera can record. For example, Direction or heading can only be detected by many GPS units only if they are level to the horizon and orientated in same direction as the camera lens.
GeorgeMy Nikonian Galleries
Well there is very little information about hooking up your D3100 with GPS devices - and after much research I thought I would share the method that I went with.First off, I opted to not use any of the "wired" methods as I think its inconvenient to do so for many reasons (I don't like taking up my hot shoe with things other than a flash, I hate holding a camera with wires hanging everywhere, etc..) In addition, most of the "wired" options are outrageously priced. And most of the "wired" units are powered by your camera's battery and thus are a nasty little power drain. I went with the bluetooth option - A bluetooth adapter + bluetooth GPS receiver. This option will assure you MUCH less battery drain because the GPS receiver has its own power source. You'll only be powering the adapter which uses very little of your batteryThis is how I did it:I bought a bluetooth adapter that plugs into the GPS connection slot - it was called the AK-N90 and I bought it for $39.99 @ http://www.semsons.com/akblgpsadfor.html - the device is very small and it is still easy to handle the camera with it plugged in - personally, I hardly notice it. As stated above, the power drain is extremely small in that you would have to try very hard to notice the extra power drain on the battery.Then I bought the GlobalSat BT-368i Bluetooth GPS Receiver for $24.00 @ http://www.amazon.com/gp/product/B0018YZ836/ref=oh_details_o00_s00_i00 This GPS receiver is TINY. Haven't had any issues with it - battery lasts all day for me thus far. Just put it in your front pocket or something and move along. Total cost for this option: $64.00 - Thus far it has worked perfectly all the time. Got a trip to Italy coming up, I'll let everyone know how it holds up over there. Either way, as it stands now, I would highly recommend this option. This option should also work well on the D90.


text: Greg Sestero Greg Sestero is a French-American actor, producer, and writer. He costarred in the cult phenomenon The Room. Become a Fan × BECOME A FAN Get updates about Greg Sestero and recommended reads from Simon & Schuster. Plus, get a FREE eBook when you sign up! Email Address Free eBook available to NEW subscribers only. Offer redeemable at Bookshout.com. Code expires 3/31/15. See full terms and conditions. By clicking SIGN UP, I acknowledge that I have read and agreed to the Privacy Policy and the Terms of Use. Books by this Author The Disaster Artist By Greg Sestero and Tom Bissell The Disaster Artist Special Signed Edition By Greg Sestero and Tom Bissell Video THE DISASTER ARTIST: My Life Inside THE ROOM, the Greatest Bad Movie Ever Made Greg Sestero, co-star and key player in the making of THE ROOM, tells the behind-the-scenes story. Link: http://videos.simonandschuster.com:80/THE-DISASTER-ARTIST-My-Life-Inside-THE-ROOM-the/2682958345001 Get a FREE eBook when you join our mailing list. Plus, receive updates about Greg Sestero,recommended reads and more from Simon & Schuster. Email Address Free eBook available to NEW subscribers only. Offer redeemable at Bookshout.com. Code expires 3/31/15.See full terms and conditions and this month's choices. Greg Sestero in the News