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Apollo Hotel
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How has this hotel rated in the past?
- Continental Breakfast
- Room service
- Restaurant
- Bar/lounge
Photos of Apollo Hotel
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TravelPod Member ReviewsApollo Hotel Athens
Welllllll, I would have not given it the 3 star rating it has otherwise it was a great hotel, nice people, but not much more than a hostel with your own 3x5 bathroom and 8x10 room. Very clean though. Side step into shower and to sit on toilet. Free breakfast (I hope so) It looks like what is not eaten is saved for next day and selection table is like at home you set out plates and bowls so guests can help themselves. Its free so again what can you say. WiFi is free and works great from your room.
This review is the subjective opinion of a TravelPod member and not of TravelPod.com.
Historical Traveler Reviews Apollo Hotel Athens
Das Apollo war großartig, sauber, nettes Personal
Mir hat der Aufenthalt im Apollo Hotel in Athen sehr gut gefallen. Es liegt in der Nähe der U-Bahnstation Metaxagorio. Die Zimmer waren sauber und mit Fernseher, Klimaanlage, großartigen Betten, Kühlschrank und Safe ausgestattet.
Das Frühstück war großartig. Auf dem Dach des Gebäudes gab es eine Terrasse mit einer Bar. Es war dort oben sehr schön und romantisch, man konnte auf ganz Athen blicken und die Akropolis bei Nacht ist einfach nur super.
Das gesamte Personal war sehr freundlich und das Internet sehr günstig.
Ich war mit meiner Mutter für 9 Tage hier und es gab nie einen Grund, sich über etwas zu beschweren.
Draußen war es über 45 Grad Celsius heiß, die Klimaanlage war also ein Muss.
Ich würde definitiv wieder in dieses Hotel gehen :).
Apollo Hotel: quanto rumore!!!!
Non tornerei più all'Hotel Apollo perchè le stanze al primo e al se condo piano ( questa è stata la nostra esperienza) erano esposte al traffico notturno di Atene. In pratica era esattamente come dormire sulla strada:tutta notte frenate, motori, clacson!!!!!!
Detto questo ( e non è di poco conto, visto che riposare in viaggio è importante....) l'hotel è molto pulito, il personale gentile ( imperdibile la signora del turno del giorno, deliziosamente speciale) e la colazione accettabile, anche se appena nei limiti.
Gli arredi e i bagni sono un capitolo un po' a parte. Un po' vecchi e antiquati, però puliti.
La fermata della metro è vicina, però per andare in centro occorre comunque cambiare, ad es. a Omonia. e c'è un treno ogni 10 minuti, quindi si perde un po' di tempo nei trasporti.
Ho alloggiato diverse volte ad Atene: se si riesce a trovare hotel più centrali, da cui raggiungere il centro a piedi, o almeno sulla linea blu, che porta sempre in centro e anche all'aeroporto ( molto strategica) si guadagna tempo. Ma vanno prenotati con mesi di anticipo....
Beau,bon et pas cher
chambre tres propre et petite douche comme partout en grece (j'ai fais 6 hotels un peu partout dans les iles) .Les lits sont propres et confortables.Femme de chambre tres tres tres ordonnée.Le premier soir apres 15 heures de vol et d'attente dans des aeroports.je ne fais que vider la valise n'importe comment pour prendre des vetements et le lendemain, je rentre et tout est tres bien placé (on aurais dit ma mere).
Le petit déjeuner etait bien surtout la salle toute vitrée. Le metro est juste a coté et 3 stations pour arriver a l'acropole.
Le quartier de l'autre coté de la rue laisse a désiré mais derriere l'hotel c'est tres bien. Épicerie au coin de la rue.Notre chambre au 5ieme etait avec balcon et pas trop bruyante puisque haute. La dame a la reception etait tres gentille.
Je recommande cet hotel a tous car le rapport qualité/prix est tres bien et pour 3 stations de metro ca ne vaut pas la peine de payer plus pour etre a Plaka.
The Apollo was great. Clean, nice staff.
I loved staying at the Apollo Hotel, in Athens. It was very close to the Metaxagorio Underground station. It had clean, nice rooms with TV, A/C, great beds, refrigerator, safe (included). The breakfast was great, the top of the building was a patio with a bar that is so romantic and gorgeous, you can see all of Athens and the Acropolis at night is amazing. All of the staff is very friendly and there is internet that is cheap. Me and my Mom stayed there 9 days and never were dissatisfied at all. The outside temp. was 110 degrees (or 40-45), so the A/C was necessary. I would definitely stay there again. : )
Nice located
We're a young gay couple and stayed at Apollo Hotel for 2 nights, before going to Mykonos.
The Hotel was very nice, and very well located. About 1 or 2 minutes from the Metro.
We had a room with a great view to the Acropole. There's some noise outside all night, but, tired of walking as we were, we fall asleep as we touch the bed!
We think that the breakfast was nice! A little bit of everything for every tastes.
The personal was very friendly and helpful.
A nice hotel to stay.
Good place to stay if you're watching your money
The hotel staff is very friendly and accomodating. The rooms are small (the smallest shower in the world!) but clean. Wifi if you go to the lobby. Make sure to ask for a room in the back. The ones in the front are extremely noisy. A good deal for the money. The location is nothing special but if you're looking for something under $100, this works very well.
Great Hotel!
Great Hotel. Ideally located close to metro and within walking distance from Omonia.
Rooms were clean and tidy and the housekeepers were amazing each day, arranging things perfectly.
Only downside is that the Hotel is on busy street, so people may find noise hard to sleep thru. I had no problem.
Try the Greek taverna behind the Hotel opposite...lovely food at a cheap price.
Great hotel!!!!
We just returned from Athens, and had a great experience with this hotel! The reception staff was amazingly helpful, especially the day receptionist. We had a problem with a tour group that we had booked a day trip with, and the receptionist very kindly dealt with the situation with the Greek only speaking staff of the tour company. Rooms were clean, and had a great view of the Acropolis. Better service than some 5 star resorts I have stayed in.
Great hotel!
Excellent view of the Acropolis from our room. Close to metro, train, supermarket, laundromat and kiosks. Internet available in lobby (with charge). Very friendly and helpful staff - Dimitrios was wonderful, with plently of tips and advice. Can get a bit noisy as the rooms overlook a main route for morning commuters.
Complain about this hotel
Living in Hotel Apollo is the worst experience during our holiday in Greece. We fell very disappointed with Hotel Apollo. Details is described as follows:
We booked a double room in hotel.de and expected it would provide a wonderful night at our last night in Greece, since the introduction said there was balcony and also we could have a good view over Acropolis in the Roof Garden. I should say it is not the case at all. No balcony, no any wonderful view, the worst is its awful service. Everything came from one of the staff of this hotel. He showed his impoliteness to us from the first beginning. When we came into the hotel and want to check in at 6 pm on September 6, 2006. He used a strain of interogation to ask our personal information. He even teased us when we asked him about whether it was free of the baggage deposit in the hotel. Maybe we didn't understand him properly, then he answer us rudely:"We are very rich. We don't need your money". It was just the beginning. At that evening, his impoliteness didn't draw any back. About 10 pm, we told him that we need a bottle of boiled water. He said:"it is an problem. No one in the kitchen now." I just asked him whether he can help us to deal with it. He refused us firmly. He even said:"This is Europe. We don't provide you any boiled water. No european asks boiled water in the hotel. Only the people from Asia like China, Hong Kong, Taiwan...". (almost the original words from him). At that moment, we were really irritated with his words and his attitude to the customer. This is the so-called "service" in this hotel. He refused to give any service probably partly because he didn't want to do that partly because we are Asian. As he mentioned, this is Europe, it is no need for him to take care of our requests. However, no hotel in Greece has done anything like that to us before that. We stayed in 3 hotels (2 in Athen and 1 in Nafplion) in Greece before that night. We asked boiled water from all hotels and they were all very kind to serve us. Of course, we couldn't accept his explaination and the unhappy quarrel happened between us afterward. Not only didn't this staff appologize to us, but also he was fully out of control. He shouted to us that "In such price, what kind of service can you expect?". Meanwhile, He threaten us that he would call the police and he plied with us when we wanted to go back to our room.
TripAdvisor Reviews Apollo Hotel Athens
Travel Blogs from Athens
Dining at Alatsi Restaurant
... am after quality and taste and I am extremely picky! Cretan cuisine is popular worldwide and happens to be one of my favorite.
LOCATION: The restaurant is located behind the Hilton on the narrow side street of Mihalakopoulou overlooking the small park. If you come by train, get off at Evagelismos metro station.
AMBIENCE: A pleasant and relaxed ambience, casual interior with a separate protected patio for smokers. If you are ...
More in 48 hrs than most ppl see in their lives
... Amanda and I patted each other on the back for beating these people here and actually enjoying 45 minutes or so without the pandemonium that comes with massive throngs of tourists.
We walked down the north slope of the Acropolis and ended up finding our way to the Agora. When we were up at the top, this was one of the places the tour guide had pointed out, mentioning that the Acropolis was the religious center and that the Agora was the political ...
Trip to Delphi
... seem to be into solar energy here, every apartment rooftop, no matter how derelict, seemed to have as many solar panels as Sky dishes, if not more. We travelled into quite a mountainous region, through a Greek ski resort town to where Delphi is located - it is astounding how far people must have trekked to spend time at a sanctuary for their gods. Delphi ...
It's Everybody's birthday
... attempted to get in another when he dropped his keys down a drain. So we're standing in the middle of the road with cabs now banking up behind us, horns beeping. An executive decision was needed so I wrenched open the door of another cab and said " we're going with you." He didn't know where he was going either but we eventually made it back to the hotel. Slept like logs. Anyway on to Athens. Dubai airport is a futuristic nightmare, ...
Day 21, Topdeck day 16. Patras to Athens
... in the bar until dinner. Dinner was fairly average, buffet style. There were cannelloni things that seriously tasted like a McDonald's cheeseburger! After dinner we had drinks on the terrace bar. This was the night that Lisa got a little drunk and sprayed a fire extinguisher in our bedroom. I can tell you, that **** doesn't taste ...


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Convert Lat Long to UTM
Type the latitude and longitude values to convert into UTM (Universal Transverse Mercator) coordinate system.
Lat Long0,0
Map Mouse Over Location0,0
What is UTM?
UTM is the abbreviation for the Universal Transverse Mercator which identifies a location independently from the vertical position.
- Easting: the x coordinate
- Northing: the y coordinate
- You can press ENTER to calculate UTM in the lat long input box.
- If you click on the UTM easting and UTM northing input box, it will auto select the value.
You can also get the lat long and the UTM values by clicking on the map.
Recent Comments
Nice one, super
Very Good to use also in norwegian as a tool in rescue mission
Very good
upto 20 meter resolution .. good to use as base map.
What is the datum in this converting (from lat/long to UTM)? Use datum = ETRS89 or datum=WGS 84?
very nice map
Super Easy to use. Loving the new click on the image to get Easting and Northing option.
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subset: commoncrawl


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Jackson News
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What would have done??
This is a family matter, but one that is causing some marriage problems. We have a family member that we have had some conflict with the past 6 years, but we still get along and are civil to each other. We recently received a graduation announcement from this family with no note of a graduation party...only the commencemnet announcement. I checked with a few other family members thinking they were having a party later, but they all told us they rc'd an extra note inside the commencement announcement regarding a party. I first thought possibly it fell out when I opened the envelope, but the party note was the size of a post card. I realize this was very possibly an oversight, but seeing that we have had conflicts in the past, I did not feel right calling and asking about it. I sent a card and money to the graduate and we did not attend the party and now these family members are very cold to my husband and I. Did we do the correct thing? Thanks for your input.
I don't understand yet, either. 1) Have they told you they're mad because you didn't attend the party? 2) if yes, did you tell them that you didn't get an invitation? 3) if yes, what did they say?
You say they know why you didn't attend (the party??? or the ceremony???), and they ddn't come forward with any explanation. That sounds as though they didn't hear it from you. Is all of this conversation happening throughout the family but not between you (and husband) and the person in question?
Why not just call and say you feel some tension and try to get to the bottom of the apparent hurt feelings (theirs and yours)?


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Patriots avoid upset, beat Midgets in OT
One team had the No. 2 ranking in Class A girls basketball. The other only had one win on the season.
Nevertheless, the one-win Dickinson High girls basketball team pushed the second-ranked Bismarck Century into overtime.
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Hoaxes Throughout History
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Hoaxes of 2014
Hoaxes of 2014
Mothers Against Videogame Addiction and Violence — Is your child receiving poor grades in school in every subject except Computer Education? Is your child spending a lot of time in their bedroom and at home? Then he or she may be suffering from videogame addiction. Mothers Against Videogame Addiction and Violence (MAVAV) is a group that's trying to educate parents about this growing threat. Check out some of the articles on their site, such as "E3: The Evil Entertainment Expo" or "EverQuest: A Threat to Society?" I could imagine a group…
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Why Do Some People Take Longer For Antibodies To Appear For Hepatitis C
Here's a taste of what TheBody.com has to offer on this topic:
Ask the Experts
Hepatitis C window periodHi, please could you tell me the correct window period for Hepatitis C, I have received conflicting answers from different people and different websites of how long it would normally take for antibodies to show up in a blood test? Some say 6-12...
Response from Barbara McGovern, M.D.
Tufts University School of Medicine...The reason it is confusing is that the majority of people do develop antibodies by 12 weeks...However, there is a small minority of people that develop antibodies between 12 and 24 weeks. Rarely, does a Read more »
Reactive Hepatitis C ABHi,Thank you for taking my question. What would cause a person to have reactive hepatitis C... Read more »
Hepatitis C occupational exposureThank you for your initial response. I have a follow up question. Originally after the spit in... Read more »
FAQs for the Public: Hepatitis C... Overview What is hepatitis? What is the difference between hepatitis A, hepatitis B, and hepatitis C? What is hepatitis C? Statistics How common is acute hepatitis C in the United States? How common is chronic Read more »
Boomers May Be Last Boom of Hepatitis C... A new study of U.S. blood donors shows a "strikingly lower prevalence" of hepatitis C virus (HCV) compared with 1992-93, according to lead researcher Dr. Edward Murphy of the University of California-San Francisco. Read more »
Hepatitis C and HIV... What Is Hepatitis C? How Is It Diagnosed? How Is Hep C Treated? Can Hep C Be Prevented? Hep C and HIV Together The Bottom Line Read more »
Rapid Liver Fibrosis Occurring More Frequently Among HIV-Infected Men Newly Coinfected With Hepatitis C...Mt. Sinai School of Medicine in Manhattan. My presentation is describing accelerated liver fibrosis in the outbreak of acute hepatitis C in HIV-infected men, 1 which had not been previously described. These were otherwise pretty healthy men... Read more »
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Hepatitis C
Posted by hopefulindy, 1 Reply...have been exposed to Hep C. If someone is infected with Hep c and HIV at the same time would that slow the production of HIV antibodies? That is what I am being told by my health care provider but he doesn't seem to know his ass from a whole in... Read more »
That Coinfection Debate
Posted by Anonymous, 1 Reply...also many who say that it will. Even Dr. Bob has seems confused in his opinion on the matter: 4/10/06 "Even coinfection with hepatitis C would not affect results out this far" However I also specifically remember him telling another person... Read more »
Infectious or Intoxication Diseases?
Posted by Anonymous...AIDS, Hepatitis C, BSE: Infectious or Intoxication Diseases? Claus Khnlein, Germany English translation and English edit by Jrgen Faas, Kathy Read more »
When should I test?
Posted by Shauna99, 7 RepliesI recently got out of a relationship with a guy who was a former IV drug user. We practiced safe sex almost every time, but there was one or two times when it just happened. We last had sex about 2 months ago. I need to know when I should be... Read more »
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See the last paragraph. Apple and Goodle MUST approve BlackBerry's BBM application on their OS or else...
June 13, 2013 at 12:59 pm PT
As the European Union's antitrust investigation into Google's search business draws closer to settlement, another probe of another of the company's businesses appears to be in the offing.
The European Commission, the EU's antitrust watchdog, is reportedly investigating claims that Google used anticompetitive means to boost its Android operating system's market share. According to documents cited by the Financial Times, which broke the story, the probe was inspired by the allegations of rivals like Microsoft and Nokia, which claim Google has been licensing Android to mobile device manufacturers below cost and making demands about the placement of its various services on their handsets. Filed in April, the complaint accuses Google of using Android "as a deceptive way to build advantages for key Google apps in 70 percent of the smartphones shipped today."
Sources described the probe as informal, and it's unclear if anything will come of it. That said, there's plenty of precedent for such preliminary efforts to expand into full-blown investigations if the agency finds merit in the allegations. And given criticism of the EC's provisional antitrust settlement with Google, EU competition commissioner Joaquín Almunia will likely be reviewing the probe's findings with a very keen eye.
Google declined comment on the probe, but issued a statement generally disputing the allegations leveled against it. "Android is an open platform that fosters competition. Handset makers, carriers and consumers can decide how to use Android, including which applications they want to use."
BlackBerry is open while the others want to control your lives...
In the current "scandal" that everything we do is spied on by gov, Blackberry is the only company in the world you can trust!
7 billion humans around the world will come to the same conclusion. Security was a primary and privacy were the reasonsI left iOS and Google Android.
BBM Text, Voice and Video on iOS and Android will create a huge global social network that BlackBerry can monetized over time like BlackBerry Money in Indonesia.


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Mercury Q&AAsk Your Question
All 2005 Mercury Monterey questions
no heat in van blowing cold air out vents
car has bad knock. some steering wheel vibration when breaking. check engine light is now on. also the car continues to cruse at a low rate of 5 or 10 miles an hour. what can I expect the repairs to be done and pricing. thank you.
battery keeps dying & buttons in front up top that control the psd stoped workin
I've had the van for a year (bought used in Oct13 its now Oct14) I've replaced the batter...
I just bought a 05 mercury Monterey starts fine but sounds really sluggish
At idle I'm kinda leaning towards the converter being plugged up or egr may be bad dash says ...
Drivers side Damper motor removal .
The van has dual climate control and rear air . I took battery cable off for ten minutes to see i...
malfunctioning radio and cd player after battery replaced
every time vehicle is started
why would it shut off going down road
i was told it was fuel pump done that and still shutting off
How to remove dashboard
Trying to retrieve gift card that fell between front windshield and dashboard
Noise when it first starts and goes away in a spit second
When the car sets overnight and is started, there's a sound like a power steering pump that i...
Both PSD's will not open, by hand or power
No codes in my husband's scanner. The doors make locking sounds when we hit the power buttons...
Misfire cylinder 4, it can get pretty severe. Violently shaking the van.
We change the oil and it goes away for a few months. We change the synthetic oil about every 2 mo...
The passenger front power window stop working
The driver side window works fine
what is causing noises up front underneath when i go over bumps?
i hear clanking noises when i go over bumps. it sounds like it is up front. mechanics have trie...
brake pedal squeaks when pressed on 2005 mercury monterey
brakes are good. the sound is just a pain! sounds like something needs WD 40 but where.
05 mercury monterey p1336 what else can cause
Replaced both the syncronizer camshaft sensor, and crankshaft sensor.
This week's top 10
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ESPN Speed World
Release History
|ESPN Speed World
|Sony Imagesoft
|North America
|01st January 1994
Game Overview
ESPN Speed World is a sports game developed by Park Place Productions and released on Sega Genesis.
Similar Games
- NBA Jam (2.05m)
- NBA Jam Tournament Edition (1.11m)
- J-League Pro Striker 2 (0.04m)
- Barkley: Shut Up and Jam! (0.00m)
- Skitchin' (0.00m)
- Davis Cup Tennis (0.00m)
- James 'Buster' Douglas Knockout Boxing (0.00m)
- NBA Showdown '94 (0.00m)
- Barkley: Shut Up and Jam 2 (0.00m)
- Slam Dunk: Kyougou Makkou Taiketsu! (0.00m)
- Devil's Course (0.00m)
- Jammit (0.00m)
- NCAA Football (0.00m)
- Bass Masters Classic (0.00m)
- Speedball 2: Brutal Deluxe (0.00m)
- Dick Vitale's Awesome Baby! College Hoops (0.00m)
- Jennifer Capriati Tennis (0.00m)
- NFL '95 (0.00m)
- Bass Masters Classic: Pro Edition (0.00m)
- Sports Talk Baseball (0.00m)
External Links
- ESPN Speed World on CoolROM


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MPs are great at either saying nothing using lots of words, or saying plenty and over-complicating things in the hope we miss the prominent information…. but nothing gets past clever, dedicated, intelligent campaigners
Archive for 'Energy, Equity & Environment'
MPs are great at either saying nothing using lots of words, or saying plenty and over-complicating things in the hope we miss the prominent information…. but nothing gets past clever, dedicated, intelligent campaigners
Crossposted from the World Wildlife Fund
Thank you!
Hundreds of thousands of you drew the line, and told UK oil company Soco to leave Virunga and it worked! Theyve committed to end their operations in Virunga, Africas oldest national park and remain out of all World Heritage Sites.
Each and every one of the[…]
This short video was made by some of my favorite eco warriors from the island of Malta. Although just a mere dot in the Mediterranean Malta is making its mark on the global stage in permaculture and innovative ways of growing food organically and sustainably not least because of is rocky landscape and its people's[…]Read more →
The Breaking the Frame gathering, which took place on 2-5th May 2014 is a step towards creating a new politics of technology. The gathering brought together campaigns on the technology politics of food, energy/climate/ environment, work/economics/austerity, the military, the internet, surveillance health and gender, as well as trade unionists, radical scientists, artists and developers of[…]Read more →
I was asked to livestream the Powershift 2014 conference learning more about other groups working on the threat of Climate Change. I am encouraged that various groups are continuing to link up, to fight against the corporate propaganda. One good lesson reiterated today. Instead of concentrating on convincing sceptics of the fight against Ecocide, attention[…]Read more →
The Occupy London Energy, Equity & Environment working group (EEE) decided towards the beginning of this year to focus on fracking and food.
On April 5th, discussion moved to the Transatlantic Trade and Investment Partnership (TTIP) and how this proposed deal to hand further power to corporations will affect fracking, food, and so much[…]
The question was asked about this new collaboration between Shell and Nerc. Good or bad?
Shelling out for NERC? Comments from a couple of the Economics WG members.
From Clive Menzies.
It's not an area I've researched in depth but Shell has a pretty appalling environmental track record.
The positive spin is to[…]
Fossil Free Nativity celebrates C of E disinvestment in 2014.
by Westley Ingram.
In 2014 the Church of England is considering disinvestment from fossil fuels. This is part of a campaign to get Churches and other great institutions to withdraw their support from businesses whose business is ecocide.
This is good news! Despite a disappointing[…]
From the farmyard to fracking, and beyond
By Mandy Pins Perkins. Occupy Bournemouth and Balcombe Community Protection Camp Protector.
We're blessed to be staying on an organic dairy farm at present, where wildlife and fauna seem to literally sing with joy every moment of the day. Yesterday afternoon, I felt called to go outside to[…]


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Clarion Hotel Marina & Conference Center
30 Lake Shore Drive East
Dunkirk, NY 14048
Phone: (716) 366-8350
Fax: (716) 366-8899
Entertainment Venues
Mickey Rats Beach Club & Captain Kidds Restaurant
Located by the beach, Mickey Rats Beach Club & Captain Kidds Restaurant is a perfect getaway for the weekend. Captain Kidds is the restaurant that has a diverse menu but is especially known for its seafood. Overlooking the beach, you can enjoy some great fish, a cool summer drink and good live music. The wraps and burritos are also a must-try. You can even host your own party or luncheon here as this place provide space for banquets and private events. Enjoy beach volleyball, tunes of the DJ and dancing at Mickey Rats which is the nightclub. Great beverages, spirits and a perky crowd defines this club where the fun never stops. So make sure you put this place in your weekend agenda.
8934 Lake Shore Road
Angola, NY 14006
+1 716 549 5782
Earn valuable points toward free nights.


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Latest exercise equipment Stories
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2014 Deep Research Report on Global and China Treadmill Industry is the new market research report added to the online library of DeepResearchReports.com. Dallas,
Anyone who watches ABC's "Extreme Weight Loss" wonders if there are any tips or tricks not shown on camera.
Athletes Can Replace Chest Strap Monitoring with Mio LINK for $99, New Device Transmits Heart Rate Data to Mio GO App to Create Virtual Outdoor Interactive Exercise Experience for Training Indoors
New LifeTrak Products Enable Consumers to Sync Data via Bluetooth® Smart to Track ECG-Accurate Heart Rate, Automatic Sleep Detection, Steps and Calorie Burn, with Smartphone Notifications - All From
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- The practice of two or more parties jointly purchasing all or part of a butchered cow and dividing the meat between them.


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Winnipeg Free Press - PRINT EDITION
'Likely' tornado rips through Pipestone
HEAVY storms -- including what a meteorologist says was likely a tornado -- ripped through southwestern Manitoba Saturday evening, with reports of overturned campers and a demolished arena.
The path of destruction started in the Pipestone area and continued east towards Hartney.
"The radar indication at that time showed a rotation indicative of a possible tornado," said Rob Paola, severe weather meteorologist for Environment Canada. "Nothing is confirmed yet, but it's quite likely there was a tornado in Pipestone."
Paola said the Winnipeg weather office has received reports half the town's arena was flattened and numerous homes and the town hall suffered roof damage. As well, several campers were overturned.
Heavy rain, hail and more reports of damage came in from the Hartney area, Paola said.
The storm system was weakening as the night progressed, but Environment Canada was expecting severe weather in the Morden area last night.
Sheila Campbell, who along with her husband, Kendon, operates a cattle farm north of nearby Reston, said they were working the fields Saturday evening and tried to return home amid the chaotic weather.
"We tried to get to the house but we couldn't because of the flood" on the road to their house, she said Saturday evening. "The water coming down the field was just like a river."
The hail was intense, she added. "It wasn't really big, but it lasted for about 15 minutes. It just kept coming and coming."
"It was like a huge snowstorm where there'd be like five inches of snow on the ground. It's still there three hours later."
She said the hay crop on their 600-acre operation, along with many crops in the area, is likely destroyed.
-- staff
Republished from the Winnipeg Free Press print edition July 14, 2013 0
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text:
In a silver stain method for protein in gels utilizing treatment with a reducing agent followed by treatment with a silver salt and actuating irradiation, the improvement comprising the use of a reducing agent consisting essentially of dithiothreitol in an amount effective to stain the protein but keep background staining to a minimum.
1. In a silver stain method for protein in gels utilizing treatment with a reducing agent followed by treatment with a silver salt and actuating irradiation, the improvement comprising
the use of a reducing agent consisting essentially of dithiothreitol in an amount effective to stain the protein but keep background staining to a minimum.
2. The improvement of claim 1 wherein the dithiothreitol is used in a concentration of from about 2.0 to about 10.0 μg/ml and the protein in gel being treated is soaked therein for a time of about 15 to about 45 minutes.
3. The improvement of claim 2 wherein the concentration is from 4.0 to 6.0 μg/ml and the time is 25 to 35 minutes.
4. The improvement of claim 3 wherein the concentration is about 5.0 μg/ml and the time is about 30 minutes.
5. In a silver stain method for protein in gels utilizing treatment with a reducing agent followed by treatment with a silver salt, the improvement comprising
the use of a reducing agent consisting essentially of dithiothreitol in an amount effective to stain the protein but keep background staining to a minimum.
6. The improvement of claim 5 wherein the dithiothreitol is used in a concentration of from about 2.0 to about 10.0 μg/ml and the protein in gel being treated is soaked therein for a time of about 15 to about 45 minutes.
7. The improvement of claim 6 wherein the concentration is from 4.0 to 6.0 μg/ml and the time is 25 to 35 minutes.
8. The improvement of claim 7 wherein the concentration is about 5.0 μg/ml and the time is about 30 minutes.
1. Field of the Invention
This invention relates to improved ultra-sensitive metallic silver stains for proteins/polypeptides, especially when fixed in synthetic gels, particularly polyacrylamide gels.
2. Description of the Prior Art
Detection and characterization of polypeptides is of fundamental importance to many areas of biology and clinical medicine. In some endeavors, such as genetic screening for mutational events, monitoring for patho-physiologic changes in disease states, and the diagnosis of genetic diseases, the efficiency of the search is directly proportional to the number of polypeptides that can be detected and characterized in cellular extracts of body fluids. Additionally polypeptides, hormones, etc., that are present in trace amounts are often of great importance for various medical reasons.
Electrophoresis (defined generally as the movement of charged particles in solution under the influence of an electrical field), is a primary laboratory detection and characterization technique, especially useful for polypeptides and other macromolecules, such as nucleic acids. It is also useful in separating small particles such as viruses, cells, sub-cellular organelles and organic molecules such as steroids and amino acids.
Continuing developments in two-dimensional gel electrophoresis have provided the capability of resolving thousands of polypeptides from complex biological mixtures. However, the inability to detect polypeptides present in low concentration has limited the application of this technology, particularly in clinical screening for pathological states, endocrinology, mammalian metabolism, developmental biology, and immunology.
Because the improved gel electrophoretic techniques greatly increase polypeptide resolution, visual detection methods employing conventional polypeptide dyes are no longer adequate.
The most commonly used conventional polypeptide stain is Coomassie Blue, which may be considered as a prototype. Dyes of this type are mainly dependent upon the electrostatic attraction between dye and polypeptide, stabilized by van der Wall's forces. In fact, Coomassie Blue and a variety of other dyes exhibit particular affinities for polypeptides of specific charge. Coomassie Blue, an acidic dye, stains basic polypeptides most intensely, while crystal violet is the most effective stain for acidic polypeptides. Other dyes for which quantitative aspects of staining have been investigated include Amido Black, Fast Green, and Fe2+ -bathophen-nanthroline sulfonate. In contrast, the Remazol Brilliant Blue R method depends on a covalent bond between dye and polypeptide. With Coomassie Blue, linearity has been found, by staining for 30 minutes in 1.5 mm diameter gels, in the polypeptide concentration range of 0.05-2 μg using the parameter of relative spot area. Staining for 60 rather than 30 minutes may result in an increase in the slope of the area/concentration relationship and nonlinearity due to saturation above 1 μg. Fluorescamine can react with terminal and ε-amino groups of polypeptides in gels to achieve a sensitivity at least equal to that of Coomassie Blue, with linearity from 1 to 7 μg "per spot". MDPF (2-methoxy-2-4-diphenyl-3(2H)-furanone) may be used to label polypeptides fluorescently prior to electrophoresis, with linearity from 10 ng to 10 μg of protein. However fluorescent staining of polypeptides prior to electrophoresis may alter their electrophoretic patterns.
An assortment of other techniques which do not require modification of proteins/polypeptides prior to electrophoresis also exist. These include densitometric scanning for absorbance at 280 nm, binding of radiolabelled or fluorescent ligands such as concanavalin A to glycoproteins, binding of antisera to polypeptides at the gel surface, and staining of specific polypeptide moieties including carbohydrate sidechains with PAS, sulfhydryl groups with 5,5'-dithiobis (2-nitrobenzoic acid), copper polypeptides with cyanide-tetrazolium, cadmium polypeptides with dipyridyl-ferrous iodide and Ca2+ -polypeptides with 45 Ca autoradiography.
Radioactive detection techniques offer a higher degree of sensitivity than the stains but are often impractical to use. In vivo radiolabelling may alter cellular metabolism and it may be impossible to label certain human polypeptides. In vitro radiolabelling has the disadvantage that it might alter the electrophoretic mobility of polypeptides. Furthermore, radioactive reagents sometimes prove too expensive and long exposure to detect trace polypeptide may result in the problem of "autoradiographic spreading".
The above staining methods, moreover, are difficult to perform, hazardous, time consuming, and unless the polypeptides are heavily labeled, lack the sensitivity to detect proteins present in low or trace concentrations. A problem arises, for example, with body fluids, such as cerebrospinal and amniotic fluids, which are often difficult to obtain in quantity and frequently contain certain abundant proteins which cause distortion of electrophoretic patterns when sufficient sample is analyzed to observe specific trace polypeptides.
Recently, highly sensitive silver strain methods for polypeptides in polyacrylamide gels have been developed. These methods have the disadvantages of being too wasteful of silver and/or being too complicated, and in most instances are less sensitive or reproducible than the improved method of this invention, although more sensitive than non-silver stains.
[Ref. 1] Kerenyi and Gallyas, in "A Highly Sensitive Method for Demonstrating Proteins in Electrophoretic, Immuno-electrophoretic and Immuno-diffusion Preparations", Clin. Chim. Acta, 38, 465-467 (1972) disclose a silver stain for proteins in agar gel in which the gel is immersed in potassium ferrocyanide, and then in a two solution developer containing sodium carbonate and water in the first solution and ammonium nitrate, silver nitrate, tungsto-silicic acid, and formaldehyde in the second solution. The possibility of using polyacrylamide gel is mentioned.
[Ref. 2] Kereyni and Gallyas, in "Uber Probleme der Quantitiven Auswertung der mit Physikalischer Entwicklung Versilberten Agarelektrophoretogramme", Clin. Chim. Acta, 47, 425-436 (1973) continued the study of the silver stain disclosed in 1972, above. Artifacts developed during the staining, whose avoidance is discussed.
[Ref. 3] Veerheecke, in "Agargel Electrophoresis of Unconcentrated Cerebrospinal Fluid", J. Neurol., 209, 59-63 (1975) discloses silver staining in agar gel utilizing two solutions after immersion of the protein-containing gel in potassium ferrocyanide. The first solution contains sodium carbonate in water, the second solution contains ammonium nitrate, water, formaldehyde, and tungsto-silicic acid as well as silver nitrate. The results reported are mixed, although generally favorable. Mention is made that the method of Kerenyi and Gallyas (1972), supra, of which this was a replication, did not appear to have found widespread acceptance, possibly because discrete bands in the gamma region of the electropherogram could not be detected and because numerous artifacts were experienced. Veerheecke himself experienced difficulties with bands in several regions.
[Ref. 4] Karcher, Lowenthal and Van Soom, in "Cerebrospinal Fluid Proteins Electrophoresis without Prior Concentration", Acta neurol. Belg., 79, 335-337 (1979), disclose silver staining utilizing two solutions after immersion of the protein-containing gel in potassium ferrocyanide. The first solution contains sodium carbonate, the second solution contains ammonium nitrate, water, formaldehyde, and tungsto-silicic acid as well as silver nitrate. The disclosure concludes that the stain is comparable to that obtained for conventional electrophoresis staining with amido-black, working with concentrated cerebrospinal fluid.
[Ref. 5] Switzer, Merril and Shifrin, in "A Highly Sensitive Silver Strain for Detecting Proteins and Peptides in Polyacrylamide Gels", Anal. Biochem, 98, 231-237 (1979), discloses a silver stain in which the proteins are fixed by soaking of the gel in various methanol/acetic acid mixtures for at least 2.5 hours, soaking the gel in a paraformaldehyde solution for 0.5 hours, placing the gel in a cupric nitrate/silver nitrate solution for at least 0.5 hours, placing the gel in a diammine solution (a mixture of silver nitrate, NaOH, NH4 OH, and ethanol) for 10 min., and twice reducing the gel strain with formaldehyde and citric acid. The stain was found to be 100 times more sensitive than Coomassie Blue and comparable to autoradiography.
[Ref. 6] Merril, Switzer, and Van Keuren, in "Trace Polypeptides in Cellular Extracts and Human Body Fluids Detected by Two-Dimensional Electrophoresis and a Highly Sensitive Silver Stain", Proc. Natl. Acad. Sci. U.S.A., 76, 4335-4339 (1979), utilized the stain disclosed by Reference 5, above. Some potential clinical applications were demonstrated as well as that the stain was more sensitive than Coomassie Blue, and less expensive and more rapid than autoradiography.
[Ref. 7] Oakley, Kirsch and Morris, in "A Simplified Ultrasensitive Silver Stain for Detecting Proteins in Polyacrylamide Gels", Anal. Biochem. 105, 361-363 (1980), discloses an adaptation of the silver stain first disclosed in Reference 5, above. The disclosed process utilizes (1) soaking the gel in glutaraldehyde for 30 minutes, (2) rinsing and soaking the gel in water for at least 2 hours, (3) adding ammoniacal silver solution (a mixture of NH4 OH, NAOH, and AgNO3), (4) transferring the gel to a mixture of citric acid and formaldehyde, and (6) washing in water for at least 1 hour. The stated advantages are simplification of the original procedure, elimination of the cupric-silver nitrate step, and reduction of the amount of silver required.
[Ref. 8] Nerril, Donau and Goldman, in Anal. Biochem., 110:201-207 (1981) and [Ref. 9] Merril, Goldman, Sedman and Ebert, in Science, 211:1437-1438 (1981), both disclose silver stains particularly useful for detecting proteins in trace quantities and for staining two-dimensional electrophoresced gels. However, there appears to be considerable variation in sensitivity from gel to gel and some proteins which stain well with Coomassie Blue do not stain with these silver stains. Further, these stain methods require a source of activating irradiation such as high intensity light.
[Ref. 10] Sun and Green, in Cell 9:511-521 (1976) have found that treating tissues with 2-mercaptoethanol (HSCH2 CH2 OH) immediately prior to silver impregnation in histological stains gave much more reproducible results. Mercaptoethanol has known use as a water-soluble reducing agent and as a non-nitrogenous sulfhydryl reagent in the investigation of proteins [see the "Condensed Chemical Dictionary" 6th ed., Reinhold Pub., N.Y., 1961].
It has been found that the use of dithiothreitol to treat gels prior to silver nitrate treatment, in an otherwise conventional ultrasensitive silver stain, results in staining patterns that are more consistently reproducible and desireably more closely resemble those obtained with Coomassie Blue. For example, in using the sensitive silver stain procedure of Reference 9, dithiothreitol treatment replaces the dichromate step. An important and completely unexpected further result of the use of dithiothreitol, is that the need for activating light irradiation is generally eliminated.
Dithiothreitol [D,L-theo-1,4-dimercapto-2,3-butanediol; Cleland's reagent; theo-2,3-dihydroxy1,4-dithiolbutane] is mentioned in The Merck Index, 9th ed., Merck & Co., Inc., Rahway, N.J., U.S.A. (1976) as being used as a protective agent for SH groups, and is mentioned in Aldrichimica Acta, 4:33 (1971) as a thiol reagent. This compound is listed in the 1981-1982 Aldrich Catalog Handbook of Fine Chemicals, Aldrich Chemical Co., Milwaukee, Wis., U.S.A. (1980).
As employed in this invention, dithiothreitol acts as a reducing agent to effect "photoreversal" and avoid silver staining of non-proteins. Dithiothreitol has been found to be an unexpected and superior replacement for known silver stain oxidizing agents such as potassium ferricyanide and dichromates. Functionally related reducing agents such as 2-mercaptoethanol (see Reference 10) and chloral hydrate have similar effects. However, when these functionally related reducing agents were tried, it was found that only the dithiothreitol of this invention was effective at very low concentrations, and only these concentrations were sufficiently low to keep background staining to a minimum.
It is not known why the use of a known reducing agent in place of a known oxidizing agent should yield an improved result, contrary to what might have been expected. Another unexpected result, is that the light irradiation required in prior art silver stains discussed above, may be completely eliminated. This greatly reduces the equipment needed to perform the silver stains, although there may be some instances in which light irradiation is useful in the improved silver stains of this invention.
The improved silver stains according to this invention are performed sequentially as follows:
Step 1. Prefix the gel in 50% methanol/10% acetic acid for 30 minutes, followed by 5% methanol/7% acetic acid for 30 minutes.
Step 2. Fix the gel for 30 minutes in 10% glutaraldehyde.
Step 3. Rinse the gel in distilled water. It is most convenient to soak the gel in a large volume of water overnight, followed by a fresh water rinse the next day for 30 minutes. Alternatively, the gel may be washed in running deionized water, or several changes of water, for 2 hours.
Step 4. Soak the gel in a reducing agent consisting essentially of 5.0 μg/ml dithiothreitol for 30 minutes. This step, which replaces treatment with oxidizing agents such as the dichromates disclosed in the prior art, is the critical improvement of this invention over the prior art.
Step 5. Pour off the dithiothreitol solution, and without rinsing, add 0.1% silver nitrate and soak for 30 minutes. This step is another improved aspect of this invention, in that no actuating irradiation, such as exposure to intense light, is necessary or preferred.
Step 6. Rinse the gel once rapidly with distilled water and then twice rapidly with developer. Soak in developer (50 μl of 37% formaldehyde in 100 ml 3% sodium carbonate) until the desired level of staining is attained. Staining is stopped by adding 5 ml of 2.3 M citric acid directly to the developer and agitating for 10 minutes. This solution is then discarded and the gel is washed several times in distilled water over a 30 minute period. For storage, it is preferred to soak the gel for 10 minutes in 0.03% sodium carbonate (to prevent bleaching), and then to seal the gel in plastic bags, cellophane, or the like.
Gentle but thorough agitation is highly recommended throughout the above procedure. The times in the above procedures are approximate and times of 10 minutes may vary by �5 minutes, times of 30 minutes�10 minutes and times of 2 hours�30 minutes, unless otherwise indicated. The quantities and identities of reagents used in the above procedure are not critical and may be varied as disclosed in the art, with the notable exception of the dithiothreitol of Step 5. The use of the dithiothreitol constitutes one of the two critical improvements of this invention. As disclosed herein, substitution for the dithiothreitol by functionally related compounds such as 2-mercaptoethanol or chloral hydrate does not yield satisfactory status, because only dithiothreitol is effective at sufficiently low concentrations as to keep background staining to a minimum. The concentration of the dithiothreitol as used in Step 4, therefore also constitutes a critical aspect of this invention. A dithiothreitol concentration of from about 2.0 to about 10.0 μg/ml is permissible, with 4.0 to 6.0 μg/ml being preferred and about 5.0 μg/ml being optimum. The soaking with the dithiothreitol solution may be for about 15 to about 45 minutes, with 25 to 35 minutes being preferred and about 30 minutes being optimum.
Preferably, the gels are fixed and stained in polyethylene containers which have been cleaned with nitric acid. The same container may be used throughout the procedure. Typically, for gels of 1 mm�9 mm�13 cm dimensions, all volumes are 100 ml, except for the 10% glutaraldehyde which is 50 ml. These volumes should be adjusted accordingly if different size gels are used. Particular attention should be paid to the volumes of the carbonate and citric acid solutions, which must be balanced to bring pH to about 7. If the pH remains too high, the reaction will not stop, and if the pH falls too low, the gel will bleach.
All gel compositions suitable for use in electrophoresis may be used in this invention. Typical gels are polyacrylamide, agarose, and cellulose acetate. Polyacrylamide is preferred, and it may be assumed that all references to "gel" denote a 10% polyacrylamide gel, unless indicated otherwise.
As with other silver stain methods, the gels utilized are fragile. They should be handled only with rinsed plastic gloves to avoid stained fingerprints on the gel. Another source of contamination that can cause spurious staining is dust in the gel solutions. It is advisable that all solutions be filtered through microporous filters prior to use.
In the course of replicating published silver stains for gels, disclosed in the discussed references, it was found that certain proteins fail to show any staining, while the staining of other proteins varies considerable from gel to gel. For example, D. discoideum spore coat protein sp 96 strains with Coomassie Blue but not with the silver staining methods of References 7 or 8. This protein failed to stain with either silver stain in at least twenty attempts. When molecular weight marker proteins are stained, both silver stains showed reduced sensitivity to insulin B chain, and in this gel the method of Reference 8 showed little sensitivity to cytochrome C. Occasionally, the method Reference 7 has been found to give a staining pattern with insulin B chain and bovine serum albumin which consists of a ring staining surrounding an unstained band. In some cases with this procedure conalbumin exhibits negative staining, in that an unstained area considerably lighter than the background is found in place of a stained band. The procedure of Reference 8 could not be replicated in its staining of whole cell proteins. A few bands were extremely dark, while the bulk of the cellular proteins stain considerably less well than with Coomassie Blue.
Variable and unreliable staining reactions have been observed with histological silver stains; however, Reference 10 discloses that treating tissues with 2-mercaptoethanol immediately prior to silver impregnation gives much more reproducible results, and is discussed previously.
Examples of actual procedures according to this invention that were utilized in preparing silver stains are as follows:
1. 50% methanol, 10% acetic acid--30 min.
2. 5% methanol, 7% acetic acid--30 min.
3. 10% glutaraldehyde--30 min.
4. deionized water, several changes--2 hrs. to overnight.
5. 5 μg/ml dithiothreitol--30 min.
6. 0.1% silver nitrate--30 min.
7. rinse rapidly once with distilled water, then twice rapidly with a small amount of developer.
8. developer--until staining reaches desired intensity.
9. add 5 ml 2.3 M citric acid directly to the developer (solution will fizz)--10 min.
10. several rinses in distilled water over a 30 min. period.
11. 0.03% sodium carbonate (to prevent bleaching)--10 min.
The developer was 50 μl 37% formaldehyde in 100 ml 3% sodium carbonate.
Using the above stain, it was found that sp 96 (see above) stained well, as did all of the molecular weight marker proteins. The total cellular protein pattern resembled that of Coomassie Blue. The stain was found to be highly reproducible.
Among the advantages of the improved silver stains of this invention, are: (1) they use significantly less silver than the prior art stains; (2) they do not require special high-intensity light sources; (3) unlike ammoniacal silver stains, they do not employ unstable or potentially explosive solutions; and (4) there is virtually no surface deposition of silver, substantially reducing background staining.
The invention method is at least as sensitive as the previous published methods. Sensitivities of selected proteins are: 0.042 ng/mm2 for bovine serum albumin, 0.083 ng/mm2 for ovalbumin, and 0.17 ng/mm2 for cytochrome C. These values compare favorably to those of References 7 and 8.
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Tru Def Shows His Inner Royalty
By Jeremy Hallock
By James Khubiar
Why You Should Be Excited About Dallas Music
By Observer Staff
Daniel Markham's Pretty Bitchin'
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Tags: Keep Tampa Bay Beautiful, America Recycles Day
America Recycles Day is coming up in November. Sustainable Living covers recycling here in the Tampa Bay area with three guests that are involved with local recycling, plus they're Keep Tampa Bay Beautiful partners, one of the best local environmental stewardship groups. Our guests were Keep Tampa Bay Beautiful Development and Community Relations Director Pat DePlasco, the Director for Hillsborough County's Solid Waste Department Kim Byers and the Gove...Be the first to comment
Poll suggests Greenlight Pinellas transit referendum could lose big listen10/27/14 Seán Kinane
WMNF Drive-Time News Monday Listen to this entire show:
Tags: Greenlight Pinellas, Pinellas County, elections, ballot initiatives, transit, Transportation
Greenlight Pinellas could be heading toward a blowout defeat; a new poll released late Friday by St. Pete Polls finds that Pinellas County voters reject the proposed sales tax increase for transit by a twelve point margin.
WMNF News interviewed Matt Florell, president of St. Pete Polls.
Their current poll shows the Greenlight Pinellas referendum being opposed by 54% of likely voters.
Tuesday we'll hear t...Be the first to comment
Political Science professor discusses the three proposed amendments to the Florida Constitution listen10/27/14 Rob Lorei
Radioactivity: Live Call-In (Monday) Listen to this entire show:
Tags: Frank Alcock, Florida Constitution, New College
Today on Radioactivity, Rob Lorei speaks to Frank Alcock an associate professor of political science at New College about the three proposed amendments to the Florida Constitution that are on the ballot this November.
FMI: www.flamendments.comBe the first to comment
Bill Clinton urges Democrats to vote as he stumps for Charlie Crist in Tampa listen10/27/14 Samuel Johnson
WMNF Drive-Time News Monday Listen to this entire show:
Tags: Charlie Crist, Bill Clinton, Rick Scott, election, Democrats, Tampa, Hillary Clinton, kathy castor, Bill Nelson, Annette Taddeo, VOTE, Florida
Bill Clinton urges Tampa to the vote out and vote for Charlie Crist for governor. The ex-president stumped for and with other Democrats Saturday at Cyrus Greene Park in Tampa. Bill Clinton's visit brought star appeal and high approval ratings to Crist's campaign.
Bill Clinton followed a line-up of Democrats which among others included Representative Kathy Castor, Senator Bill Nelson, Charlie Crist's running mate Annette Taddeo and Charlie Crist himself. The message from all the speakers w...Be the first to comment
Coalition of Immokalee Workers unveils Fair Food Label listen10/24/14 Seán Kinane
WMNF Drive-Time News Friday Listen to this entire show:
Tags: CIW, Coaliton of Immokalee Workers, labor, unions, farmworkers, fair food, food
If you want to buy tomatoes that have been grown with the rights of farmworkers in mind, you now have a label to look for.
Friday the Coalition of Immokalee Workers unveiled a green Fair Food Label.
WMNF's Seán Kinane interviewed Gerardo Reyes Chavez, a member of the Coalition of Immokalee Workers.
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Poynter president on why they're hosting African journalists when USF St. Pete would not listen10/24/14 Seán Kinane
WMNF Drive-Time News Friday Listen to this entire show:
Tags: Ebola, Media, Poynter Institute, USF, USF St. Pete, St. Petersburg, Africa, Journalism
This week the University of South Florida at St. Petersburg decided to rescind its invitation to a group of African journalists because of student and faculty fears of Ebola; but the nearby Poynter Institute decided to host the journalists instead.
To find out what was behind those decisions and how the media is covering the Ebola outbreak, WMNF spoke with Tim Franklin, president of the Poynter Institute.
<iframe width="100%" height="166" scrolling="no" frameborder="no" src="https://w....Be the first to comment
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Is it just me not clicking all of the discussions here or is Salt MIA lately?
I just started peeking in here not that long ago and have appreciated his vast knowledge, but felt too bold emailing him inquiring about his absence. Hopefully, it's just an extended summer vacation-last post I could find was July 28, so it's just a month now.
This post was edited by olychick on Thu, Aug 28, 14 at 17:45
I emailed him & am happy to report he replied with this happy news...getting PAID to do what we all love to do here! Wishing him the BEST in his new endeavor!
Hi Rosemarie,
All's well, thanks for asking. I'm just getting paid to do identifications now, so don't have the time to do the freebies much any more. If I ever decide to stop you'll probably see me on Gardenweb more often.
Pass this along if you wouldn't mind.


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The Mysterious Cities of Gold - Part 2
View Single Post
May 24 2013, 10:40 PM
Location: England
Re: The Mysterious Cities of Gold - Part 2
T J wrote:
Now we're getting somewhere! I have a feeling they are about the get their new medallion center pieces, here's hoping!
That should make things interesting ......
Esteban: Yay!, we've found another city of gold. Oh, wait. We don't have the sun part of the medallions anymore. We can't open the doors.
Zares: What? You stupid kids! You had me chase you all the way to Asia for nothing.
In other news, Mysterious Cities of Gold is getting a Wii U / and 3DS game.
- based on the anime of the same name
- coming from Neko Entertainment
- being developed under the supervision of Flaoua
- switch between three heroes (Esteban, Zia and Tao)
- features a top-down view
- due out this Fall
And the subbed MKVs for episodes 06 and 07 are now available.
View Public Profile
Send a private message to Stephen!
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Description: Elevated view of many horse-drawn vehicles on a main street in an unidentified rural village, perhaps at a farmer's fair. A crowd of men is standing outsid...
Description: East Water Street from Kirby House. View of buildings and street. Street car in the distance.
Date: 1948-06-12
Description: Chester Roberts, Milwaukee, campaigning for State Treasurer at the Republican State Convention.
Date: 1936
Description: Label submitted to the state of Wisconsin for trademark registration. "Five O'clock Club Beer, A. Gettelman Brewing Company." The label features the silhou...
Date: date unknown
Description: Quarter-length portrait of Rezeau Blanchard Brown, Milwaukee gas engineer.
Date: 1905 ca.
Description: Exterior of classical-style building. Facade has fluted columns, decorated capitals, ornate railings, and a wide stairway leading to the entrance. A man is...
Description: A view of Commission Row, known for its fresh produce markets, looking northwest on N. Broadway between E. Buffalo and E. Chicago Streets.
Date: 1985-02-21
Description: The Auer Avenue School's safety cadet team standing in front of the school building.
Description: Polland at the Mid-Summer Festival on the Milwaukee lakefront, with the boys of Nick Ehr family in the foreground. This was just after the successful Zeidl...
Date: 1849
Description: This map shows lot and block numbers, wards, local streets, railroads, some landowners' names, Milwaukee River, Menomonee River, and part of Lake Michigan....
Description: Runner reaching first base with coach signaling for him to stop. Defensive players and the umpire look on. A portion of the crowd in the stands is visible...
Date: 1958-01-30
Description: Milwaukee Braves General Manager John Quinn (left) and manager Fred Haney (right) accept awards at the 5th Annual Diamond Dinner, hosted by the Milwaukee C...
Date: 1916 ca.
Description: The first aid department at a Pawling & Harnischfeger plant. A woman sitting at the desk working on the "daily report first aid room." On the left is a sin...
Date: 1914
Description: Quarter-length side-profile studio portrait of Lewis Sherman, Milwaukee physician and pharmacist.
Date: 1915 before
Description: View looking east from Third Street, down the avenue. Buildings line the road, with signs and advertisements. Roads and sidewalks are crowded with horse-...
Date: 1958-11-05
Description: Women campaign workers call voters to support the Labor Political League and vote for candidates sympathetic to Labor issues.
Date: 1900 ca.
Description: Exterior of building. Caption below gives the name of the building and the line "Totally Destroyed by Fire June 4 '05." A group of people stand outside th...
Date: 1940-01-01
Description: Engine #934 of the Chicago, Milwaukee, St. Paul and Pacific Railroad Company.
Date: 1955-07-12 ca.
Description: Comedic actor Joe E. Brown holding a baseball, with an unidentified man standing behind him..
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text:
Lego Doctor Who Is Officially Happening in 2015
Lego Doctor Who set confirmed for later this year!
Lego Doctor Who set confirmed for later this year!
Lego Doctor Who sets are still under review.
Lego Doctor Who another brick closer as first set enters review phase.
Lego sorts out licence rights on Doctor Who.
Costume still nowhere to be seen.
And Paul McGann.
Now you can kill him over and over.
Peg Soldier, Cybermen, Bearded Doctor and Cybermats.
Your very own pet Bitey.
The Flesh Doctor, The Astronaut, River Song and more.
Stetsons are cool.
More pics and details on the the Doctor Who construction sets.
Well, it's basically Lego Doctor Who without the licence.
Silurian Warrior, Angel Bob, Roman Auton, Amy Pond, The Eleventh Doctor (with Fez!) and Cyberman Pandorica Guard coming soon.
Three new action figure sets this December at Forbidden Planet.


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Sears 1/2 hp Garage door
I had a thunder storm and the following morning the garaage door open, but when I press to shut the light blinked. There is no light present in the senors. Could someone tell me if its the sensor or the circut board. Help!
Thank you in advance.
Sounds like your logic board might be going on the fritz (power sureg??). If you think you might want to replace it yourself, I can tell you where NOT to buy it. I recently bought one from Ontario Supply in New York from their web site for $60. Unfortunately, it has turned out to be defective and they won't refund my purchase price even though I sent the defective unit back at my own cost. So, don't buy from them, they don't deal fairly in my opinion. I think you're best off just calling a local repair service and let them replace the board. If you replace the board yourself and it does not work, you're just going to end up calling a repair service anyway. Ontario Supply operates under the following web sites:
In my opinion, you should avoid this company


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Cupertino News
Local news for Cupertino, CA continually updated from thousands of sources on the web.
City of Cupertino Hosts Earth & Arbor Day Festival
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Jay Z's streamin' biz takes dead aim at Apple
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Cupertino International Poetry Cantos
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From Palo Alto to State Bird
Thursday Mar 26 | Palo Alto Weekly | Comment?
Minerva Surgical Inc. - Medical Equipment - Deals and Alliances Profile - New Report Available
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Senate approves water legislation to address drought, flooding
Wednesday Mar 25 | Los Angeles Times | Comment?
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Water district board to consider recycled water for Apple Campus 2 and other South Bay sites
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Steve Wozniak shares thoughts on robot apocalypse and a potential Apple car
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Great teachers: In Cupertino and San Jose, Mr. Garland and Mrs. Davies changed lives
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Descriptions of House District 17A and 17B in west central Minn.
WILLMAR -- The Minnesota legislative districts have changed in west central Minnesota as part of the 2012 special redistricting decision issued today. House District 17A includes all of Swift County and parts of Chippewa, Kandiyohi and Renville counties. District 17B covers the city of Willmar and several other towns and townships in Kandiyohi County.
House District 17A:
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Top Linksmain detailsbiographyby votesphoto galleryquotes
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Helen North Beardsley (Character)
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- Yours, Mine & Ours (2005) Played by Rene Russo (as Helen North)
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The Nadowli Kaleo District is one of the Eleven (11) Districts in the Upper West Region of Ghana. Nadaowl District is located in the heart of the Upper West region of Ghana. It lies between latitude 11' 30' and 10' 20' north and longitude 3' 10' and 2'10' west. The Administrative capital of the District is Nadowli. The District shares boundaries with Jirapa District to the North, to the South with Wa west, to the East with Daffiama Bussie Issa District and to the West with Ivory Coast.
The Nadowli –Kaleo Distrit Assembly as part of measures to improve security in the district has embarked on measures to open additional police stations in some selected communities in the district.
One hundred low tension electricity poles have been procured and distributed to some selected communities in the Nadowli-Kaleo District.
The Ghana Social Opportunities Project (GSOP) continues to impact positively in the lives of many deprived communities in Ghana.
Residents of Sombo and Nator in the Nadowli Kaleo District in the Upper West Region will soon be relieved of their water challenges following measures to increase water supply in the two communities.
The Nadowli/Kaleo district assembly is likely to sit for an adhoc meeting soon following the appointment of Mr. John Bosco Bomansan as the new DCE for the assembly.


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Bayou City History
A blog about Houston's past with J.R. Gonzales
Posts filtered on Category
Dylan & Petty: A night of confessions
Ben DeSoto : Chronicle file Bob Dylan and Tom Petty onstage at the Southern Star Amphitheater (Astroworld), June 20, 1986. Fulfilling a request by BCH reader intrepid, here's a June 1986 picture of a plugged-in Bob Dylan onstage at the Southern Star Amphitheater. Dylan teamed up with Tom Petty and the Heartbreakers that summer for […] [Read More]
The Shamrock Hotel (Part 5 of 5)
Buster Dean : Chronicle file Bystanders watch as the Shamrock Hilton's demolition enters its ninth day, June 1987. Check-out time. Our story comes to an end. In 1985, the Shamrock property was sold to the Texas Medical Center. As Stephen Fox notes in the Handbook of Texas, the hotel's size, its location and the growth […] [Read More]
The Shamrock Hotel (Part 4 of 5)
Seems most people I talk to about the Shamrock have fond memories of its massive pool. In keeping with that theme, here's a pool-related picture. Story Sloane Gallery Bathing beauties at the Shamrock, 1950s. Chronicle file From the April 7, 1963, edition of the Houston Chronicle: "The Shamrock Hilton Corkettes will be one of the […] [Read More]
The Shamrock Hotel (Part 3 of 5)
Chronicle file Hotel magnate Conrad Hilton took over the mortgage payments and operations of the Shamrock Hotel in late 1954. From the Feb. 17, 1956, edition of the Houston Chronicle: "Sign men are changing the name of the Shamrock Hotel to that of Shamrock Hilton. First they put up the Hilton. Then they started on […] [Read More]
The Shamrock Hotel (Part 2 of 5)
"I tell you, you've never seen anything like it," said Houston Chronicle reporter Martin Dreyer. "There was glitter, glitter, everywhere," the Houston Post reported. "There has been nothing like the Shamrock opening in all of Houston's turbulent, fast-paced history," wrote Houston Press reporter Ben Kaplan. Here are some more little tidbits from the chaotic celebrations, […] [Read More]
The Shamrock Hotel (Part 1 of 5)
Chronicle file United Fund of Houston campaign, Oct. 6, 1952 Once upon a time — 59 years ago to be exact — Houston and Hollywood society turned out for a celebration. Actually, "celebration" may be understating it a bit. This was a bash — or "pandemonium" as the Houston Press described it. The occasion? The […] [Read More]
Alfred the Great
When looking through old newspapers, it's hard to ignore a picture of a chimp in a top hat. Here's what the Houston Post wrote in its March 2, 1913, edition when Alfred the Great — "The Highest Salaried Animal in the World" — visited Houston: "Coming to the Majestic this week is the greatest living […] [Read More]
When the Stones rolled into town
Buster Dean : Chronicle file The Rolling Stones perform at the Sam Houston Coliseum, July 19, 1978. You may recall that one of the first blog posts at Bayou City History 2.0 was about a radio spot advertising the Rolling Stones' July 11, 1966, show at the Sam Houston Coliseum. Well, while digging through the […] [Read More]
Actress refuses to get upstaged by hecklers
Houston Press headline, March 13, 1913 Sometimes we tend to think of the "good ol' days" as being just that — good or genteel. Public rudeness? That's a modern-day malady, some might say. That, of course, isn't true, as this March 13, 1913, Houston Press article indicates. Noted stage actress Valeska Suratt was in Houston […] [Read More]
One movie, two theaters
Newspaper advertisement from July 6, 1919. While researching something recently, I came across a peculiar item that ran in the Houston Press. In the July 3, 1919, edition a columnist noted that Charlie Chaplin's upcoming film Sunnyside would play at two theaters in Houston. It was apparently the first time in Houston history that two […] [Read More]
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Funny animal antics and post-Apocalyptical dread abound in Neathery's web comic series. Read More »
Home » Tag Archives: funny animals
Tag Archives: funny animals
Graphic Novel Review: Dungeon Twilight – Volume Three: The New Centurions by Sfar, Trondheim, Kerascoet, and Obion
Sfar and Trondheim's comic funny animal fantasy epic continues. Read More »
Graphic Novel: Dungeon: The Early Years — Volume Two: Innocence Lost by Joann Sfar, Lewis Trondheim and Christophe Blain
Both a send-up and a celebration of swashbuckling fantasy. Read More »


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RE: What To Do With Retired Admirals & Generals?
By CDRSalamander
Let a thousand voices sing. The good Dr.'s points are fine for a pre-IT world – but the educated citizen can find out all they want now days via the intertubes. After awhile, those retired officers that only seem to chirp up during election cycles and then sound like a talking-point on loop lose their credibility. The marketplace of ideas can be efficient like that
The modern version of this came with retired Admiral William Crowe, who stood next to then Gov. Clinton in the middle of the the '92 election and gave him the top cover he needed at the right point – got an appt. at the Court of St. James as a thanks and we were off to the races. Say what you want about the politics of it – and you know me and USNI, I would prefer if you didn't – but at least ADM Crowe was a class act about it all. I don't think you can say that about much of what we have seen this decade.
The key is for others to speak up in response as much as needed – and for other retired officers to counter the excess and call their peers to account. There was a lot of credibility lost in the last election – I think the excess will take care of itself as we go forward. The best antidote for speech you think is wrong is more speech; just be an adult about it.
There is also a line of thought that not all opinion is political – though it may go against a politician's opinion.
To wander into my point – I will, ahem, form up with FOD on this one subject as I read Dr. Snider's 'lil paper as a discussion of a "Food Trough" problem; but I don't think some pre-fall of the Berlin Wall central registry idea is the solution. Don't make people qualify to keep their 1st Amendment rights; they earned it.
Now, a cooling off period of say 5 years after retirement for all GOFO or SES from working for any privately or publicly held company or subsidiary that conducts more than $500 million annually, adjusted yearly for inflation, with the DoD …. now were talking about sump'n!
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UDF bid to address pro-minority tilt
In an apparent move to get over the blues caused by the induction of the Indian Union Muslim League's fifth Minister into the Cabinet, Chief Minister Oommen Chandy appears to be reaching out to the Sree Narayana Dharma Paripalana Yogam (SNDP) by extending several sops to the Ezhava community.
Some of the recent Cabinet decisions are quite significant for being specific in their content. It gives clear indications about the UDF's line of thinking, particularly that of the Congress, coming as it does in the backdrop of the greater Hindu unity move spearheaded by the SNDP Yogam and the Nair Service Society against the perceived pro-minority tilt of the ruling coalition.
The most important of the decisions is the one taken by Wednesday's Cabinet to include the teachings of Sree Narayana Guru in the school curriculum up to Standard XII, excluding Standards IV and VI. This would be included in the Malayalam and social science textbooks in these ten classes.
The Chief Minister, at his customary post-Cabinet press briefing, said the government had accepted the M.K. Sanoo committee's recommendations after the Education Department and the State Council for Educational Research and Training scrutinised it.
The curriculum committee would examine the proposal and take necessary action to include it from the next academic year, he said.
Another decision taken by the Cabinet a couple of weeks ago was to sanction a Rs.3-crore grant for the proposed Sivagiri Convention Centre at Varkala. A month earlier, the Cabinet went the extra mile to de-notify three important temples in Malabar from the purview of the Malabar Devaswom Board.
The operative part of this decision is to hand over the administration of the Sreekanteswaram temple, Kasaba, in Kozhikode; Jaganatha temple, Thalassery; and the Sundaresha temple, Thallappu, in Kannur to managing committees.
The three temples, among the few revenue earning temples in Malabar, were actually notified temples under the Hindu Religious and Endowment Act prior to being brought under the MDB that came up later, testifying to their historical importance.
The Left Democratic Front government had earlier made moves to de-notify these temples but the process could not be completed as it went out of power. The UDF government apparently had capitalised on this by de-notifying the three temples.
Another major opportunity for the government will come by when the question of constituting the Travancore Devaswom Board is taken up soon.
According to reliable information, Subhash Vasu, who is being backed by the SNDP, has emerged as the frontrunner for the TDB president's post. Several names are doing the rounds but going by the current decision-making methods, the SNDP may have its way, sources said.
Keywords: Oommen Chandy, Ezhava community, Sree Narayana Guru


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CineformA high-quality video production codec that works on both Mac and PCs and can scale from standard-definition to 4k film resolution. Cineform is a wavelet codec (not a DCT codec) that is often used as a digital intermediary codec for editing video captured in formats like HDV that, because of the way they're compressed, are difficult to edit on many systems.
See also "A Complete Guide to Video Codecs" on WebVideoUniverse.com.
Perceptual computing is the ability for a computer to recognize what is going on around it. More specifically, the computer can perceive the... Read More »Apple Pay Promises to Strengthen Payment Security
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