text:
Are you Andrew J Connolly?Claim your profile
Publications (45)342.37 Total impact
Article: Enhanced Caspase Activity Contributes to Aortic Wall Remodeling and Early Aneurysm Development in a Murine Model of Marfan Syndrome.[Show abstract] [Hide abstract]
ABSTRACT: Rupture and dissection of aortic root aneurysms remain the leading causes of death in patients with the Marfan syndrome, a hereditary connective tissue disorder that affects 1 in 5000 individuals worldwide. In the present study, we use a Marfan mouse model (Fbn1(C1039G/+)) to investigate the biological importance of apoptosis during aneurysm development in Marfan syndrome.Arteriosclerosis Thrombosis and Vascular Biology 10/2014; DOI:10.1161/ATVBAHA.114.304364 · 5.53 Impact Factor
Article: Clonal analysis reveals nerve-dependent and independent roles on mammalian hind limb tissue maintenance and regeneration[Show abstract] [Hide abstract]
ABSTRACT: The requirement and influence of the peripheral nervous system on tissue replacement in mammalian appendages remain largely undefined. To explore this question, we have performed genetic lineage tracing and clonal analysis of individual cells of mouse hind limb tissues devoid of nerve supply during regeneration of the digit tip, normal maintenance, and cutaneous wound healing. We show that cellular turnover, replacement, and cellular differentiation from presumed tissue stem/progenitor cells within hind limb tissues remain largely intact independent of nerve and nerve-derived factors. However, regenerated digit tips in the absence of nerves displayed patterning defects in bone and nail matrix. These nerve-dependent phenotypes mimic clinical observations of patients with nerve damage resulting from spinal cord injury and are of significant interest for translational medicine aimed at understanding the effects of nerves on etiologies of human injury.Proceedings of the National Academy of Sciences 06/2014; 111(27). DOI:10.1073/pnas.1410097111 · 9.81 Impact Factor
Article: Assessment of Elastin Deficit in a Marfan Mouse Aneurysm Model Using an Elastin-Specific Magnetic Resonance Imaging Contrast Agent[Show abstract] [Hide abstract]
ABSTRACT: -Ascending aortic dissection and rupture remain a life-threatening complication in patients with Marfan syndrome (MFS). The extracellular matrix provides strength and elastic recoil to the aortic wall, thereby preventing radial expansion. We have previously shown that ascending aortic aneurysm formation in Marfan mice (Fbn1(C1039G/+)) is associated with decreased aortic wall elastogenesis and increased elastin breakdown. In this study, we test the feasibility of quantifying aortic wall elastin content using magnetic resonance imaging (MRI) with a gadolinium-based elastin-specific contrast agent (ESMA) in Fbn1(C1039G/+) mice. -Ascending aorta elastin content was measured in 32-week-old Fbn1(C1039G/+) mice and wild-type (WT) (n=9 and n=10, respectively) using 7T MRI with a T1-mapping sequence. Significantly lower enhancement (i.e., lower R1 values, where R1=1/T1) was detected post-ESMA in Fbn1(C1039G/+) compared to WT ascending aortas (1.15±0.07 vs. 1.36±0.05, p<0.05). Post-ESMA R1 values correlated with ascending aortic wall gadolinium content directly measured by inductively coupled mass spectroscopy (p=0.006). -Herein, we demonstrate that MRI with ESMA accurately measures elastin bound gadolinium within the aortic wall and detects a decrease in aortic wall elastin in Marfan mice compared to WT controls. This approach has translational potential for non-invasively assessing aneurysm tissue changes and risk, as well as monitoring elastin content in response to therapeutic interventions.Circulation Cardiovascular Imaging 05/2014; 7(4). DOI:10.1161/CIRCIMAGING.114.001658 · 5.80 Impact Factor
Circulation Journal 10/2013; 78(2). DOI:10.1253/circj.CJ-13-0708 · 3.69 Impact Factor
Article: Femtosecond plasma mediated laser ablation has advantages over mechanical osteotomy of cranial bone[Show abstract] [Hide abstract]
ABSTRACT: BACKGROUND: Although mechanical osteotomies are frequently made on the craniofacial skeleton, collateral thermal, and mechanical trauma to adjacent bone tissue causes cell death and may delay healing. The present study evaluated the use of plasma-mediated laser ablation using a femtosecond laser to circumvent thermal damage and improve bone regeneration. METHODS: Critical-size circular calvarial defects were created with a trephine drill bit or with a Ti:Sapphire femtosecond pulsed laser. Healing was followed using micro-CT scans for 8 weeks. Calvaria were also harvested at various time points for histological analysis. Finally, scanning electron microscopy was used to analyze the microstructure of bone tissue treated with the Ti:Sapphire laser, and compared to that treated with the trephine bur. RESULTS: Laser-created defects healed significantly faster than those created mechanically at 2, 4, and 6 weeks post-surgery. However, at 8 weeks post-surgery, there was no significant difference. In the drill osteotomy treatment group, empty osteocyte lacunae were seen to extend 699 ± 27 µm away from the edge of the defect. In marked contrast, empty osteocyte lacunae were seen to extend only 182 ± 22 µm away from the edge of the laser-created craters. Significantly less ossification and formation of irregular woven bone was noted on histological analysis for drill defects. CONCLUSIONS: We demonstrate accelerated bone healing after femtosecond laser ablation in a calvarial defect model compared to traditional mechanical drilling techniques. Improved rates of early regeneration make plasma-mediated ablation of the craniofacial skeleton advantageous for applications to osteotomy. Lasers Surg. Med. © 2012 Wiley Periodicals, Inc.Lasers in Surgery and Medicine 12/2012; 44(10). DOI:10.1002/lsm.22098 · 2.61 Impact Factor
[Show abstract] [Hide abstract]
ABSTRACT: Pluripotent cells represent a powerful tool for tissue regeneration, but their clinical utility is limited by their propensity to form teratomas. Little is known about their interaction with the surrounding niche following implantation and how this may be applied to promote survival and functional engraftment. In this study, we evaluated the ability of an osteogenic microniche consisting of a hydroxyapatite-coated, bone morphogenetic protein-2-releasing poly-l-lactic acid scaffold placed within the context of a macroenvironmental skeletal defect to guide in vivo differentiation of both embryonic and induced pluripotent stem cells. In this setting, we found de novo bone formation and participation by implanted cells in skeletal regeneration without the formation of a teratoma. This finding suggests that local cues from both the implanted scaffold/cell micro- and surrounding macroniche may act in concert to promote cellular survival and the in vivo acquisition of a terminal cell fate, thereby allowing for functional engraftment of pluripotent cells into regenerating tissue.Proceedings of the National Academy of Sciences 11/2012; 109(50). DOI:10.1073/pnas.1218052109 · 9.81 Impact Factor
Article: Microfluidic Single-Cell Analysis Shows That Porcine Induced Pluripotent Stem Cell-Derived Endothelial Cells Improve Myocardial Function by Paracrine Activation[Show abstract] [Hide abstract]
ABSTRACT: Rationale: Induced pluripotent stem cells (iPSCs) hold great promise for the development of patient-specific therapies for cardiovascular disease. However, clinical translation will require preclinical optimization and validation of large-animal iPSC models. Objective: To successfully derive endothelial cells from porcine iPSCs and demonstrate their potential utility for the treatment of myocardial ischemia. Methods and Results: Porcine adipose stromal cells were reprogrammed to generate porcine iPSCs (piPSCs). Immunohistochemistry, quantitative PCR, microarray hybridization, and angiogenic assays confirmed that piPSC-derived endothelial cells (piPSC-ECs) shared similar morphological and functional properties as endothelial cells isolated from the autologous pig aorta. To demonstrate their therapeutic potential, piPSC-ECs were transplanted into mice with myocardial infarction. Compared with control, animals transplanted with piPSC-ECs showed significant functional improvement measured by echocardiography (fractional shortening at week 4: 27.2±1.3% versus 22.3±1.1%; P<0.001) and MRI (ejection fraction at week 4: 45.8±1.3% versus 42.3±0.9%; P<0.05). Quantitative protein assays and microfluidic single-cell PCR profiling showed that piPSC-ECs released proangiogenic and antiapoptotic factors in the ischemic microenvironment, which promoted neovascularization and cardiomyocyte survival, respectively. Release of paracrine factors varied significantly among subpopulations of transplanted cells, suggesting that transplantation of specific cell populations may result in greater functional recovery. Conclusions: In summary, this is the first study to successfully differentiate piPSCs-ECs from piPSCs and demonstrate that transplantation of piPSC-ECs improved cardiac function after myocardial infarction via paracrine activation. Further development of these large animal iPSC models will yield significant insights into their therapeutic potential and accelerate the clinical translation of autologous iPSC-based therapy.Circulation Research 07/2012; 111(7):882-93. DOI:10.1161/CIRCRESAHA.112.269001 · 11.09 Impact Factor
Article: Cardiac pressure overload hypertrophy is differentially regulated by β-adrenergic receptor subtypes.[Show abstract] [Hide abstract]
ABSTRACT: In isolated myocytes, hypertrophy induced by norepinephrine is mediated via α(1)-adrenergic receptors (ARs) and not β-ARs. However, mice with deletions of both major cardiac α(1)-ARs still develop hypertrophy in response to pressure overload. Our purpose was to better define the role of β-AR subtypes in regulating cardiac hypertrophy in vivo, important given the widespread clinical use of β-AR antagonists and the likelihood that patients treated with these agents could develop conditions of further afterload stress. Mice with deletions of β(1), β(2), or both β(1)- and β(2)-ARs were subjected to transverse aortic constriction (TAC). After 3 wk, β(1)(-/-) showed a 21% increase in heart to body weight vs. sham controls, similar to wild type, whereas β(2)(-/-) developed exaggerated (49% increase) hypertrophy. Only when both β-ARs were ablated (β(1)β(2)(-/-)) was hypertrophy totally abolished. Cardiac function was preserved in all genotypes. Several known inhibitors of cardiac hypertrophy (FK506 binding protein 5, thioredoxin interacting protein, and S100A9) were upregulated in β(1)β(2)(-/-) compared with the other genotypes, whereas transforming growth factor-β(2), a positive mediator of hypertrophy was upregulated in all genotypes except the β(1)β(2)(-/-). In contrast to recent reports suggesting that angiogenesis plays a critical role in regulating cardiac hypertrophy-induced heart failure, we found no evidence that angiogenesis or its regulators (VEGF, Hif1α, and p53) play a role in compensated cardiac hypertrophy. Pressure overload hypertrophy in vivo is dependent on a coordination of signaling through both β(1)- and β(2)-ARs, mediated through several key cardiac remodeling pathways. Angiogenesis is not a prerequisite for compensated cardiac hypertrophy.AJP Heart and Circulatory Physiology 06/2011; 301(4):H1461-70. DOI:10.1152/ajpheart.00453.2010 · 4.01 Impact Factor
Article: Pretreatment with angiotensin-converting enzyme inhibitor improves doxorubicin-induced cardiomyopathy via preservation of mitochondrial function[Show abstract] [Hide abstract]
ABSTRACT: Doxorubicin is a widely used chemotherapy drug, but its application is associated with cardiotoxicity. Free radical generation and mitochondrial dysfunction are thought to contribute to doxorubicin-induced cardiac failure. Angiotensin-converting enzyme inhibitors are commonly used as cardioprotective agents and have recently been shown in clinical studies to be efficacious in the prevention of anthracycline-induced heart failure. This study evaluated a mechanism for these protective effects by testing the ability of the angiotensin-converting enzyme inhibitor enalapril to preserve mitochondrial function in a model of chronic doxorubicin treatment in rats. Sprague Dawley rats were divided into 3 groups and followed for a total of 10 weeks: (1) control-untreated, (2) doxorubicin treated, and (3) doxorubicin + enalapril treated. Doxorubicin was administered via intraperitoneal injection at weekly intervals from weeks 2 to 7. Enalapril was administered in the drinking water of the doxorubicin + enalapril group for the study duration. Doxorubicin treatment produced a significant loss in left ventricular contractility (P < .05), decrease in mitochondrial function via impairment of state-3 respiration, decrease in the cytosolic fraction of adenosine triphosphate, and up-regulation of free radical production. Enalapril significantly attenuated the decrease in percent fractional shortening (P < .05) and prevented the doxorubicin-associated reduction in respiratory efficiency and cytosolic adenosine triphosphate content (P < .05). Enalapril also abolished the robust doxorubicin-induced increase in free radical formation. Administration of enalapril attenuates doxorubicin-induced cardiac dysfunction via preservation of mitochondrial respiratory efficiency and reduction in doxorubicin-associated free radical generation.The Journal of thoracic and cardiovascular surgery 11/2010; 142(2):396-403.e3. DOI:10.1016/j.jtcvs.2010.07.097 · 3.41 Impact Factor
Article: Influences of Aortic Motion and Curvature on Vessel Expansion in Murine Experimental Aneurysms[Show abstract] [Hide abstract]
ABSTRACT: To quantitatively compare aortic curvature and motion with resulting aneurysm location, direction of expansion, and pathophysiological features in experimental abdominal aortic aneurysms (AAAs). MRI was performed at 4.7 T with the following parameters: (1) 3D acquisition for vessel geometry and (2) 2D cardiac-gated acquisition to quantify luminal motion. Male 24-week-old mice were imaged before and after AAA formation induced by angiotensin II (AngII)-filled osmotic pump implantation or infusion of elastase. AngII-induced AAAs formed near the location of maximum abdominal aortic curvature, and the leftward direction of expansion was correlated with the direction of suprarenal aortic motion. Elastase-induced AAAs formed in a region of low vessel curvature and had no repeatable direction of expansion. AngII significantly increased mean blood pressure (22.7 mm Hg, P<0.05), whereas both models showed a significant 2-fold decrease in aortic cyclic strain (P<0.05). Differences in patterns of elastin degradation and localization of fluorescent signal from protease-activated probes were also observed. The direction of AngII aneurysm expansion correlated with the direction of motion, medial elastin dissection, and adventitial remodeling. Anterior infrarenal aortic motion correlated with medial elastin degradation in elastase-induced aneurysms. Results from both models suggest a relationship between aneurysm pathological features and aortic geometry and motion.Arteriosclerosis Thrombosis and Vascular Biology 11/2010; 31(2):270-9. DOI:10.1161/ATVBAHA.110.216481 · 6.34 Impact Factor
Article: In vivo quantification of murine aortic cyclic strain, motion, and curvature: implications for abdominal aortic aneurysm growth.[Show abstract] [Hide abstract]
ABSTRACT: To develop methods to quantify cyclic strain, motion, and curvature of the murine abdominal aorta in vivo. C57BL/6J and apoE(-/-) mice underwent three-dimensional (3D) time-of-flight MR angiography to position cardiac-gated 2D slices at four locations along the abdominal aorta where circumferential cyclic strain and lumen centroid motion were calculated. From the 3D data, a centerline through the aorta was created to quantify geometric curvature at 0.1-mm intervals. Medial elastin content was quantified with histology postmortem. The location and shape of abdominal aortic aneurysms (AAAs), created from angiotensin II infusion, were evaluated qualitatively. Strain waveforms were similar at all locations and between groups. Centroid motion was significantly larger and more leftward above the renal vessels than below (P < 0.05). Maximum geometric curvature occurred slightly proximal to the right renal artery. Elastin content was similar around the circumference of the vessel. AAAs developed in the same location as the maximum curvature and grew in the same direction as vessel curvature and motion. The methods presented provide temporally and spatially resolved data quantifying murine aortic motion and curvature in vivo. This noninvasive methodology will allow serial quantification of how these parameters influence the location and direction of AAA growth.Journal of Magnetic Resonance Imaging 10/2010; 32(4):847-58. DOI:10.1002/jmri.22331 · 2.57 Impact Factor
Article: The role of recipient mast cells in acute and chronic cardiac allograft rejection in C57BL/6-KitW-sh/W-sh mice[Show abstract] [Hide abstract]
ABSTRACT: Mast cells are hypothesized to promote rejection and adverse remodeling in cardiac allografts. In contrast, it has been reported that mast cells may enhance cardiac allograft survival in rats. We used C57BL/6-Kit(W-sh/W-sh) mast cell-deficient and corresponding wild-type mice to investigate possible contributions of recipient mast cells to acute or chronic cardiac allograft rejection. FVB (H-2(q); acute rejection), or C-H-2(bm12)KhEg (H-2(bm12); chronic rejection) donor hearts were heterotopically transplanted into C57BL/6-Kit(W-sh/W-sh) (H-2(b)) or C57BL/6-Kit(+/+) (H-2(b)) mice. The degree of acute rejection was assessed at 5 days and chronic rejection, at 52 days. In the acute rejection model, donor heart vascular cell adhesion molecule-1 (VCAM-1) expression was significantly lower in C57BL/6-Kit(W-sh/W-sh) than in wild-type recipients; however, acute rejection scores, graft survival, inflammatory cells, or cytokine expression did not differ significantly. In the chronic rejection model, the number of mast cells/mm(2) of allograft tissue was significantly increased 52 days after transplantation in allografts transplanted into C57BL/6-Kit(+/+) but not C57BL/6-Kit(W-sh/W-sh) mice; however, no substantial differences were noted in graft coronary artery disease, graft inflammatory cells, or levels of graft tissue expression of cytokines or adhesion molecules. Cardiac allografts undergoing chronic rejection in wild-type C57BL/6-Kit(+/+) mice exhibit increased numbers of mast cells, but acute or chronic cardiac allograft rejection can develop in C57BL/6-Kit(W-sh/W-sh) mice even though these recipients virtually lack mast cells. These findings indicate that recipient mast cells are not required for acute or chronic cardiac allograft rejection in the models examined.The Journal of heart and lung transplantation: the official publication of the International Society for Heart Transplantation 10/2009; 29(4):401-9. DOI:10.1016/j.healun.2009.08.019 · 5.61 Impact Factor
[Show abstract] [Hide abstract]
ABSTRACT: Teratoma formation is a critical obstacle to safe clinical translation of human embryonic stem (ES) cell-based therapies in the future. As current methods of isolation are unable to yield 100% pure population of differentiated cells from a pluripotent donor source, potential development of these tumors is a significant concern. Here we used non-invasive reporter gene imaging to investigate the relationship between human ES cell number and teratoma formation in a xenogenic model of ES cell transplantation. Human ES cells (H9 line) were stably transduced with a double fusion (DF) reporter construct containing firefly luciferase and enhanced green fluorescent protein (Fluc-eGFP) driven by a human ubiquitin promoter. Immunodeficient mice received intramyocardial (n = 35) or skeletal muscle (n = 35) injection of 1 x 10(2), 1 x 10(3), 1 x 10(4), 1 x 10(5) or 1 x 10(6) DF positive ES cells suspended in saline for myocardium and Matrigel for skeletal muscle. Cell survival and proliferation were monitored via bioluminescence imaging (BLI) for an 8 week period following transplantation. Mice negative for Fluc signal after 8 weeks were followed out to day 365 to confirm tumor absence. Significantly, in this study, a minimum of 1 x 10(5) ES cells in the myocardium and 1 x 10(4) cells in the skeletal muscle was observed to be requisite for teratoma development, suggesting that human ES cell number may be a critical factor in teratoma formation. Engraftment and tumor occurrence were also observed to be highly dependent on ES cell number. We anticipate these results should yield useful insights to the safe and reliable application of human ES cell derivatives in the clinic.Cell cycle (Georgetown, Tex.) 09/2009; 8(16):2608-12. DOI:10.4161/cc.8.16.9353 · 5.24 Impact Factor
[Show abstract] [Hide abstract]
ABSTRACT: To evaluate the feasibility of reporter gene imaging in implanted human mesenchymal stem cells (MSCs) in porcine myocardium by using clinical positron emission tomography (PET)-computed tomography (CT) scanning. Animal protocols were approved by the Institutional Administrative Panel on Laboratory Animal Care. Transduction of human MSCs by using different doses of adenovirus that contained a cytomegalovirus (CMV) promoter driving the mutant herpes simplex virus type 1 thymidine kinase reporter gene (Ad-CMV-HSV1-sr39tk) was characterized in a cell culture. A total of 2.25 x 10(6) transduced (n = 5) and control nontransduced (n = 5) human MSCs were injected into the myocardium of 10 rats, and reporter gene expression in human MSCs was visualized with micro-PET by using the radiotracer 9-(4-[fluorine 18]-fluoro-3-hydroxymethylbutyl)-guanine (FHBG). Different numbers of transduced human MSCs suspended in either phosphate-buffered saline (PBS) (n = 4) or matrigel (n = 5) were injected into the myocardium of nine swine, and gene expression was visualized with a clinical PET-CT. For analysis of cell culture experiments, linear regression analyses combined with a t test were performed. To test differences in radiotracer uptake between injected and remote myocardium in both rats and swine, one-sided paired Wilcoxon tests were performed. In swine experiments, a linear regression of radiotracer uptake ratio on the number of injected transduced human MSCs was performed. In cell culture, there was a viral dose-dependent increase of gene expression and FHBG accumulation in human MSCs. Human MSC viability was 96.7% (multiplicity of infection, 250). Cardiac FHBG uptake in rats was significantly elevated (P < .0001) after human MSC injection (0.0054% injected dose [ID]/g +/- 0.0007 [standard deviation]) compared with that in the remote myocardium (0.0003% ID/g +/- 0.0001). In swine, myocardial radiotracer uptake was not elevated after injection of up to 100 x 10(6) human MSCs (PBS group). In the matrigel group, signal-to-background ratio increased to 1.87 after injection of 100 x 10(6) human MSCs and positively correlated (R(2) = 0.97, P < .001) with the number of administered human MSCs. Reporter gene imaging in human MSCs can be translated to large animals. The study highlights the importance of co-administering a "scaffold" for increasing intramyocardial retention of human MSCs.Radiology 04/2009; 252(1):117-27. DOI:10.1148/radiol.2513081616 · 6.21 Impact Factor
The Journal of Heart and Lung Transplantation 02/2009; 28(2). DOI:10.1016/j.healun.2008.11.810 · 5.61 Impact Factor
[Show abstract] [Hide abstract]
ABSTRACT: Abegrin is a monoclonal antibody to human integrin alphavbeta3, a cell adhesion molecule highly expressed on actively angiogenic endothelium and glioblastoma multiforme tumor cells. The purpose of this study was to evaluate the efficacy of a novel 90Y-Abegrin radioimmunotherapeutic agent in murine xenograft glioblastoma models with noninvasive in vivo molecular imaging modalities. A s.c. U87MG human glioblastoma xenograft model was used to determine maximum tolerated dose (MTD), biodistribution, dose response, and efficacy of 90Y-Abegrin. Antitumor efficacy was also characterized in an orthotopic U87MG and in a HT-29 colorectal cancer model, a low integrin-expressing carcinoma. Small-animal positron emission tomography imaging was used to correlate histologic findings of treatment efficacy. MTD and dose response analysis revealed 200 microCi per mouse as appropriate treatment dose with hepatic clearance and no organ toxicity. 90Y-Abegrin-treated U87MG tumor mice showed partial regression of tumor volume, with increased tumor volumes in 90Y-IgG, Abegrin, and saline groups. 18F-FDG imaging revealed a reduction of cell proliferation and metabolic activity whereas 18F-FLT reflected decreased DNA synthesis in the 90Y-Abegrin group. Ki67 analysis showed reduced proliferative index and quantitative terminal deoxynucleotidyl transferase dUTP nick-end labeling-positive analysis revealed increased DNA fragmentation and apoptosis in 90Y-Abegrin animals. CD31 and 4',6-diamidino-2-phenylindole staining showed increased vascular fragmentation and dysmorphic vessel structure in 90Y-Abegrin animals only. Orthotopic U87MG tumors treated with 90Y-Abegrin displayed reduced tumor volume. HT-29 tumors showed no significant difference among the various groups. Radioimmunotherapy with 90Y-labeled Abegrin may prove promising in the treatment of highly vascular, invasive, and heterogeneous malignant brain tumors.Clinical Cancer Research 12/2008; 14(22):7330-9. DOI:10.1158/1078-0432.CCR-08-0797 · 8.19 Impact Factor
Article: Multimodal evaluation of in vivo magnetic resonance imaging of myocardial restoration by mouse embryonic stem cells.[Show abstract] [Hide abstract]
ABSTRACT: Mouse embryonic stem cells have demonstrated potential to restore infarcted myocardium after acute myocardial infarction. Although the underlying mechanism remains controversial, magnetic resonance imaging has provided reliable in vivo assessment of functional recovery after cellular transplants. Multimodal comparison of the restorative effects of mouse embryonic stem cells and mouse embryonic fibroblasts was performed to validate magnetic resonance imaging data and provide mechanistic insight. SCID-beige mice (n = 55) underwent coronary artery ligation followed by injection of 2.5 x 10(5) mouse embryonic stem cells, 2.5 x 10(5) mouse embryonic fibroblasts, or normal saline solution. In vivo magnetic resonance imaging of myocardial restoration by mouse embryonic stem cells was evaluated by (1) in vivo pressure-volume loops, (2) in vivo bioluminescence imaging, and (3) ex vivo TaqMan (Roche Molecular Diagnostics, Pleasanton, Calif) polymerase chain reaction and immunohistologic examination. In vivo magnetic resonance imaging demonstrated significant improvement in left ventricular ejection fraction at 1 week in the mouse embryonic stem cell group. This finding was validated with (1) pressure-volume loop analysis demonstrating significantly improved systolic and diastolic functions, (2) bioluminescence imaging and polymerase chain reaction showing superior posttransplant survival of mouse embryonic stem cells, (3) immunohistologic identification of cardiac phenotype within engrafted mouse embryonic stem cells, and (4) polymerase chain reaction measuring increased expressions of angiogenic and antiapoptotic genes and decreased expressions of antifibrotic genes. This study validates in vivo magnetic resonance imaging as an effective means of evaluating the restorative potential of mouse embryonic stem cells.The Journal of thoracic and cardiovascular surgery 11/2008; 136(4):1028-1037.e1. DOI:10.1016/j.jtcvs.2007.12.053 · 3.41 Impact Factor
Article: Immunosuppressive therapy mitigates immunological rejection of human embryonic stem cell xenografts.[Show abstract] [Hide abstract]
ABSTRACT: Given their self-renewing and pluripotent capabilities, human embryonic stem cells (hESCs) are well poised as a cellular source for tissue regeneration therapy. However, the host immune response against transplanted hESCs is not well characterized. In fact, controversy remains as to whether hESCs have immune-privileged properties. To address this issue, we used in vivo bioluminescent imaging to track the fate of transplanted hESCs stably transduced with a double-fusion reporter gene consisting of firefly luciferase and enhanced GFP. We show that survival after transplant is significantly limited in immunocompetent as opposed to immunodeficient mice. Repeated transplantation of hESCs into immunocompetent hosts results in accelerated hESC death, suggesting an adaptive donor-specific immune response. Our data demonstrate that transplanted hESCs trigger robust cellular and humoral immune responses, resulting in intragraft infiltration of inflammatory cells and subsequent hESC rejection. Moreover, we have found CD4(+) T cells to be an important modulator of hESC immune-mediated rejection. Finally, we show that immunosuppressive drug regimens can mitigate the anti-hESC immune response and that a regimen of combined tacrolimus and sirolimus therapies significantly prolongs survival of hESCs for up to 28 days. Taken together, these data suggest that hESCs are immunogenic, trigger both cellular and humoral-mediated pathways, and, as a result, are rapidly rejected in xenogeneic hosts. This process can be mitigated by a combined immunosuppressive regimen as assessed by molecular imaging approaches.Proceedings of the National Academy of Sciences 10/2008; 105(35):12991-6. DOI:10.1073/pnas.0805802105 · 9.81 Impact Factor
Article: Insulin resistance is associated with a modest increase in inflammation in subcutaneous adipose tissue of moderately obese women.[Show abstract] [Hide abstract]
ABSTRACT: We have previously described differences in adipose cell size distribution and expression of genes related to adipocyte differentiation in subcutaneous abdominal fat obtained from insulin-sensitive (IS) and -resistant (IR) persons, matched for degree of moderate obesity. To determine whether other biological properties also differ between IR and IS obese individuals, we quantified markers of inflammatory activity in adipose tissue from overweight IR and IS individuals. Subcutaneous abdominal tissue was obtained from moderately obese women, divided into IR (n = 14) and IS (n = 19) subgroups by determining their steady-state plasma glucose (SSPG) concentrations during the insulin suppression test. Inflammatory activity was assessed by comparing expression of nine relevant genes and by immunohistochemical quantification of CD45- and CD68-containing cells. SSPG concentrations were approximately threefold higher in IR than in IS individuals. Expression levels of CD68, EMR1, IL8, IL6 and MCP/CCL2 mRNAs were modestly but significantly increased (p < 0.05) in IR compared with IS participants. Results of immunohistochemical staining were consistent with gene expression data, demonstrating modest differences between IR and IS individuals. Crown-like structures, in which macrophages surround single adipocytes, were rarely seen in tissue from either subgroup. A modest increase in inflammatory activity was seen in subcutaneous adipose tissue from IR compared with equally obese IS individuals. Together with previous evidence of impaired adipose cell differentiation in IR vs equally obese individuals, it appears that at least two biological processes in subcutaneous adipose tissue characterize the insulin-resistant state independent of obesity per se.Diabetologia 10/2008; 51(12):2303-8. DOI:10.1007/s00125-008-1148-z · 6.88 Impact Factor
Article: In vivo imaging of embryonic stem cells reveals patterns of survival and immune rejection following transplantation.[Show abstract] [Hide abstract]
ABSTRACT: Embryonic stem cell (ESC)-based transplantation is considered a promising novel therapy for a variety of diseases. This is bolstered by the suggested immune-privileged properties of ESCs. In this study, we used in vivo bioluminescent imaging (BLI) to non-invasively track the fate of transplanted murine ESCs (mESCs), which are stably transduced with a double fusion reporter gene consisting of firefly luciferase (FLuc) and enhanced green fluorescent protein (eGFP). Following syngeneic intramuscular transplantation of 1 x 10(6) mESCs, the cells survived and differentiated into teratomas. In contrast, allogeneic mESC transplants were infiltrated by a variety of inflammatory cells, leading to rejection within 28 days. Acceleration of rejection was observed when mESCs were allotransplanted following prior sensitization of the host. Finally, we demonstrate that the mESC derivatives were more rapidly rejected compared to undifferentiated mESCs. These data show that mESCs do not retain immune-privileged properties in vivo and are subject to immunological rejection as assessed by novel molecular imaging approaches.Stem cells and development 06/2008; 17(6):1023-9. DOI:10.1089/scd.2008.0091 · 4.15 Impact Factor
Top Journals
Stanford UniversityPalo Alto, California, United States
Stanford Medicine
Stanford, California, United States
- • Department of Pathology
- • Department of Developmental Biology
- • Division of Cardiovascular Medicine
Cardiovascular Research FoundationNew York, New York, United States
University of California, San Francisco
San Francisco, CA, United States
- • Department of Pathology
- • Division of Hospital Medicine


text:
I am so going to do this. I have quite a few shirts that no longer fit me and this would be a great way to re-purpose them. No-sew cardigan tutorial. No Sewing Cardigans, Diy Crafts, Cardigans Tutorials, Old Shirts, Diy Cardigans, Diy Clothing, Nosew Cardigans, T Shirts, Longer Fit
Photo 2 by Easily Dunn on Flickr
Use old shirts that no longer fit in this no-sew cardigan tutorial - SO EASY. #diy #cardigan
old t shirts that no longer fits into a no-sew cardigan tutorial - Easy and Love not throwing out my favs. :)
#sewing #DIY #craft #homemade Have shirts or jumpers that no longer fit? Turn them into cardigans.
Also on these boards
Related Pins
Men`s Shirt--would I be able to do this? How big would the guy have to be if my boobs are supposed to be the size of his neck?!
Cardigan conversion - great way to save sweaters from the goodwill bag! I tried this, and I get compliments on it all the time.
DIY Tutorial: Clothes Refashion / DIY No Sew, One Shoulder Shirt - Bead&Cord
Too small sweater - made into a cardigan... Wow I would have never thought of doing something like this, but I think it's a great idea! I am going to try!
tried it with an oldish shirt, and i loved it so i made a nice one. pretty easy (coming from a girl who doesn't sew), just takes a little time.
Easy upcycle: Cut open the back of an existing or thrifted top, replace with doilies, sew back together.
Super easy shirt. Just a couple seams and thats it. I was thinking of adding a bandeau at bottom so it's more mini muffin top friendly. Making it out of two silk scarfs would be awesome too.
Talk2TheTrees: How To Make An Easy AND Versatile Shirt (For Cheap!)

subset: commoncrawl


text:
Pool depth wrong but they say can't have top edge lower?
Hi. We are getting a liner pool with concrete cantelever edge, and that concrete will go right up to our existing raised paver patio off our house. The pool company knew this, but now that the concrete is starting, it turns out that we need a drain the entire length of the concrete up against our paver patio because of the angle of the concrete. Supposedly if the pool were 2 inches deeper (it's really the top of the pool being 2 inches lower in the ground that is the point) this would not be an issue. But the pool company is saying that they could not have gone deeper with it because it would create issues with the liner. I was wondering if anyone in the know out there can tell me if this is them covering up a mistake that at this point would be very costly to fix (they have the pool dug, metal walls in with concrete around the outside edge over all piping), or if there really is a legitimate reason?
I figure if it was purposeful then they'd have told us the day they dug, and they did not. Now I am spending a lot of money for how an inground pool looks to have to stare at a very long ugly drain every day. I'm quite depressed overall about this. Thanks for any help or insights!
The rule of thumb for slope on a deck is 1/4" per foot. if your pool is 6' from the patio then the slope would be 1 1/2".
Saying they couldn't lower the pool 2" more is pretty dumb. Sounds like they screwed up. Keep in mind that deck-0-drain is common for providing drainage.
How much slope are you dealing with now?
The deck has to slope away from the pool. If you have an existing deck that is attached to the house, they slope away from the house. This is to keep runoff from going back in your house or in the pool. So where the two decks meet is a low spot. This is where you need the channel drain to take the runoff away. Pretty standard.


text:
O Lord and Vivifier,
Your grace has achieved for us
all that You had spoken and promised.
Grant us access to the place of Your peace.
For You are our Vivifier,
You are our Consoler,
You are our life Remedy,
You are our Standard of Victory.
Blessed are we, O Lord,
because we have known You!
Blessed are we,
because we have believed in You!
Blessed are we,
because we bear Your wounds
and the sign of Your Blood on our contenances!
Blessed are we,
because You are our great hope!
Blessed are we,
because You are our God forever!
Four things to know about your candle on Catholic Online:
Visit, we beseech Thee, O Lord, this dwelling, and drive far from it all snares of the enemy; let Thy holy Angels dwell herein, to preserve us in peace; and let Thy blessing be upon us forever. Through Christ our Lord. Amen. continue readingMore Prayer of the Day
In the centuries old Catholic tradition, holy cards or prayer cards are small, devotional cards for the use of the faithful. Prayer cards have an endless amount of use and make wonderful gifts and keepsakes.
A Prayer For Children Holy Card
Prayer Cards
23rd Psalm Holy Card
Prayer Cards
A Christmas Blessing Holy Card
Prayer Cards
25th Jubilee Holy Card
Prayer Cards
A Marriage Prayer Holy Card
Prayer Cards
50th Jubilee Holy Card
Prayer Cards
5-Way / Chalice
Prayer Cards
A Marriage Blessing Holy Card
Prayer Cards


text:
Pronunciation: ghaytanHa'
Added on: 2012-11-29in: Add categories to ghaytanHa'
ghaytanHa' is pending pronunciation in:
- Klingon[tlh] Do you know how to pronounce ghaytanHa'?
- Random word: pep
by Google Translate English translation:
ghaytanHa' = ghaytanHa '
Modify word:
· Add languages
· Add categories
Report word:
Is there anything wrong with this word?
Please report it here.


text:
Member Since: 10/25/08Send This Person a Message Give this person a Gift Add This Person as a Friend
Undisputed PL Love DoctorBlessed to have him as a PL Friend Honest likes butter brickle great sense of humor Respectful Caring Professional True Hero Special Brother in Blue InsideOut Chief Dennis is a true proffesional with a big heart ONE OF THE BEST courage and confidence Hes often seen here displaying his knowledge Many consider UncD to be one of PLs role models your ears should be OPEN When this man speaks Displays Sincerity Integrity and Confidence Funniest Guy on Policelink
You can reach this milestone by accruing more than 50 reputation points in the last 7 days
You can reach this milestone by completing the quiz Would You Pass the Police Exam?
You can reach this milestone by completing the quiz Good Cop / Bad Cop
You can reach this milestone by completing Stew Smith's Fitness Challenge
You can reach this milestone by starting a group that is one of the top 100 most active groups.
You can reach this milestone by inviting at least 5 friends who join PoliceLink.
The first 1,000 PoliceLink members to earn one star are considered Founding Members
This user is a PoliceLink Forums Moderator
You can reach this milestone by being one of the top 100 users on the Reputation Leaderboard.
Only sworn LEOs are eligible to receive access to restricted areas.
uncledennis1: Profile Photos
uncledennis1: Friends
uncledennis1: Groups
- Job Openings
- Retired Officers Group Life after the Job
- Christian Knights of the Rose KOR
- The Warrior Code
- Missouri Police Departments
- Brotherhood of the Badge
- New York City Police
- Military Parents
- Cops Helping Cops
- Security officers vs Cops Why
- Small Town Cops
- Army Military Police Officers
- Midwest Security Officers
- International Police Collectors Group
- Reserve Police and Deputies
- Fallen Heroes
- Military Family
- Reserve Officers
- Thank a Solider Thank a Vet
- Security Officers or LEOs You be the Judge
- Death Penalty for cop killers
- Losing a loved one to death
- Officer Down Group Line of Duty Deaths
- The Army Service Group
- Missouri LEO
- The Coliseum Fight Club Part 2
uncledennis1: Quiz Results
2nd Amendment Shootout taken about 5 years ago
Pro 2nd Amendment
Copper or Hosehead taken almost 6 years ago
LEO All the Way
uncledennis1: Gifts
- Location:
- Independence, MO
- Service:
- Police Officer / Deputy Sheriff / Other Sworn Position
- Status:
- Retired (has access to L.E. restricted areas)
- Relationship Status:
- Engaged
- Hobbies:
- Football, Boxing & Golf. NYPD collector. NYPD antique holsters
- Favorite Movies:
- French Connection
- Favorite TV Shows:
- two and ahalf men
- Favorite Music:
- Rock 60's & 70's
uncledennis1: References
Chuck mac
City Of New York, Police OfficerSummary: Dennis brings a lot of experience to PL. He has a heart of gold and is a ver good friend. I would of taken him as a partner any day.
Shane "coshane220"
PoliceLink , FriendSummary: I am proud to have Shetland as one of my friends on PoliceLink. His experience in LawEnforcement makes him more than qualified to be a mentor to anybody wanting to get into this field.
Jim Bragg
G'ville PD, Just a brother LEOSummary: Shetland IS a cops cop who can lead or who can work with anyone to get the job done. An excellant example of a real Police Officer.
Johnny Etter,Jr.
N/A, N/ASummary: Uncledennis is a amazing person and I am glad to have him as a friend on policelink
Leah Carp
PL friends, sergeantSummary: Uncle D has been my friend for a few years. He is a great supporter and a wealth of information. He is honest, a person I would trust with my life! Thank you Uncle D for your service, you are amazing in my eyes!
uncledennis1: Activity
I have seen applicants in their 50's. Don't over think it. Get in shape.
Dave. Tell us how you really feel.
They will follow up on the assault charge. Once you sign the waiver they are entitled to all documents and can speak to whomever they wish. Be hone...
"Thanks bro. Hope all is well. Shetland"
Comment Wall
Add this person as a friend to write on their Comment Wall.
5 months ago
6854 Comments
Hey Boss. How's things?
5 months ago
43090 Comments
Just stopping by to say Hi. Have a safe and great week.
5 months ago
29142 Comments
Hope all is going well for you. Need to get some yard work done on my days off as the leaves are falling now. Not looking forward to the up coming Winter months.Take care.
5 months ago
3194 Comments
5 months ago
52186 Comments
What up Brother? Go KC
Have a blessed Sunday my friend.Take care.
6 months ago
11320 Comments
Dennis, Hope all is well with you. Take care, be safe & have a great weekend !! Bob P. PS. Take a gallon of Butter Brickle ice cream out of petty cash for the weekend.
6 months ago
43090 Comments
Stopping by to say Hi. Hope all is well. have a great weekend!
6 months ago
5992 Comments
6 months ago
3194 Comments
It's October and Halloween draws near - sooooooooooo I'm just saying BOO TO YOU a bit early.
Have a terrific week my friend.Take care and stay in touch.
Hello..........have a great weekend.
7 months ago
43014 Comments
Enjoy the holiday and stay safe.
7 months ago
5992 Comments


text:
Follow these tips to make renting a breeze.
Are you searching for apartments, vacation rentals or houses for rent in Dalhart? If so, you've definitely come to the right place. RealRentals.com showcases Dalhart houses for rent - posted by property managers, real estate agents, and private homeowners. Hundreds of new rental houses are added daily, so you're sure to find a great Dalhart rental house in no time. Do you have a house, or multiple houses for rent in Dalhart? Post a FREE Rental Listing and start advertising your Dalhart house, condo, townhouse, loft, patio home, duplex, mobile home, cabin or cottage - today!


text: I Dare You Not to Smile at This Huge Collection of Laughs! [VIDEO] By Laura Peterson September 9, 2011 10:44 AM Share on Facebook Share on Twitter I would normally save a find like this for a Monday, but I couldn't resist. I dare you to keep a straight face through this video. Filed Under: babies, laughing, laughter Category: Lifestyle, Video Share on Facebook Share on Twitter More Leave a comment Send to a friend! Print this page Pin it! Reddit This! Share on Tumblr! Best of Mix108 Recommended For You Around the Web


text:
By clicking the "Submit" button, you agree to Avvo's Terms of Use. Please see our Community Guidelines and Privacy Policy for information on posting to Avvo and how we collect, use, and share information you provide to us.
25700 Science Park Dr Ste 270Beachwood, OH 44122
Licensed for 39 years in OH
Go back to Lester's profile.


text:
Komodo dragon bites 2 in officeFebruary 07,2013
Komodo dragon
bites 2 in office
JAKARTA, Indonesia Two people have been hospitalized after being attacked by a giant Komodo dragon that wandered into the office of a wildlife park in eastern Indonesia.
An official at Komodo National Park, Heru Rudiharto, said Wednesday the 6½-foot-long Komodo dragon attacked a park ranger after walking into the office on Tuesday.
It then attacked another park employee who came to help him. Both were badly bitten and were evacuated to a hospital on Bali Island.
Rudiharto said the park ranger also was attacked by a Komodo dragon in 2009.
Endangered Komodo dragons can grow longer than 10 feet. Fewer than 4,000 are believed to be alive. They are found in the wild primarily on the eastern Indonesian islands of Komodo, Padar and Rinca.MORE IN Wire NewsNORMAN, Okla. Full StorySTOCKHOLM Swedish poet and Nobel Literature Prize winner Tomas Transtromer has died at age 83,... Full StorySANAA, Yemen As airstrikes in Yemen intensified on their second day Friday, Egypt and Saudi... Full Story
- Most Popular
- Most Emailed


text:
Show Biz Team Building
View Listing
Bridgestone Racing Academy
League of Rock
Canadian Outback Adventures & Events
Sound Team Building Events
Summit Team Building
Learn2 Solutions
The Aussie X
Air Combat Zone
The Winter Sports Challenge
Arctic Kingdom
Eagle's Flight
Coast Hotels Vancouver, BC, Canada
Pan Pacific Vancouver Vancouver, BC, Canada
Meetings + Conventions Calgary Calgary, AB, Canada
MPI Appoints New Canadian Sales Leader
Industry Insider: 2015 – Another Year of Challenges?
Unconventional Wisdom: Are Trade Shows Passé?
CAEM Celebrates Stars of the Show
Asked + Answered – Trade Shows NOT Passé
Fort Myers' Property Unveils New Meeting Facilities
Travel Executives say Canadian Business Market Remains Steady
San Francisco Preparing for WEC
Tourism Toronto Head Heading South to Brand USA
Scottsdale Resort Getting Makeover
— Sandy Biback
Yeah, 2 of the smartest people in the tradeshow industry have responded....
— Serge Micheli
Dear Sandy:
In your blog post, you voice your opinion that the typical...
— Lori Smith
Hi Stuart,
Thank you for your clarification. I will amend the article.
All the...
— Tamer Mecky
Congratulations to all the winners and fellow CEM Graduates.
— Stuart Taylor
Hello! As the representative for the MPI chapters in Canada, I wanted...
PHOTOS: Speakers' Spotlight Showcase
PHOTOS: CanSPEP Annual Conference
PHOTOS: Quebec Day
PHOTOS: MPI BC Education Conference
PHOTOS: CAEM Awards Gala


text:
CINCINNATI -- Two students were involved in a rape at Lighthouse Community School in Madisonville, authorities said Friday.
Both minors, a male and female student were in a room on campus alone when the assault happened, according to Superintendent Daniel Trujillo. The school immediately told authorities what happened, and called the students' parents.
Cincinnati police officers took both students in for questioning.
It was not immediately clear if the victim was physically injured.


text:
Dr. Eric E. Kline, MD
Urological Surgery
- 2525 NE 139th St Ste 220
Vancouver, WA 98686
- 2 Locations
- (360) 882-2778
Dr. Eric E. Kline graduated from the University of New Mexico School of Medicine in 2004. He works in Vancouver, WA and 1 other location and specializes in Urological Surgery. Dr. Kline is affiliated with Legacy Salmon Creek Medical Center and Peacehealth Southwest Medical Center. He speaks English and Spanish.
Gender: Male
Languages Spoken: English, Spanish
Urological Surgery
Medical School: University of New Mexico School of Medicine; Graduated 2004
*This physician has multiple office locations. See Office Information for details.


text:
About us
Contact us
Tubas / Salfit
Syria rebels in push to capture air base
Published Thursday 03/01/2013 (updated) 05/01/2013 21:29
Smoke rises from a Syrian military air base during fighting with Free
Syrian Army fighters at Taftanaz near the northern province of Idlib
Jan. 2, 2013. (Reuters/Ismail Al-Taftanazi, Shaam News Network, HO)
Yara Bayoumy
AZAZ, Syria (Reuters) -- Rebels battled on Thursday to seize an air base in northern Syria, part of a campaign to limit the air power that has given President Bashar Assad the upper hand.
More than 60,000 people have been killed in the 21-month-old uprising and civil war, the United Nations said this week, sharply raising the death toll in a conflict that shows no sign of ending.
After dramatic advances over the second half of 2012, the rebels now hold wide swathes of territory in the north and east, but are limited in exerting control because they cannot protect towns and villages from Assad's helicopters and jets.
Hundreds of fighters from rebel groups were attempting to storm the Taftanaz air base, near the northern highway that links Syria's two main cities, Aleppo and the capital Damascus.
Rebels have been besieging air bases across the north in recent weeks, in the hope this will reduce the government's power to carry out air strikes and resupply loyalist-held areas.
A rebel fighter speaking from near the Taftanaz base overnight said the base's main sections were still in loyalist hands but insurgents had managed to infiltrate and destroy a helicopter and a fighter jet on the ground.
The northern rebel Idlib Coordination Committee said the rebels had detonated a car bomb inside the base.
The government's SANA news agency said the base had not fallen and that the military had "strongly confronted an attempt by the terrorists to attack the airport from several axes, inflicting heavy losses among them and destroying their weapons and munitions."
Rami Abdulrahman, head of the opposition-aligned Syrian Observatory for Human Rights which monitors the conflict from Britain, said as many as 800 fighters were involved in the assault, including Islamists from Jabhat al-Nusra, a powerful group that Washington considers terrorists.
Taftanaz is mainly a helicopter base, used for missions to resupply army positions in the north, many of which are cut off by road because of rebel gains, as well as for dropping crude "barrel bombs" of explosives on rebel-controlled areas.
'What is the fault of the children?
Near Minakh, another northern air base that rebels have surrounded, close to the Turkish boder, government forces have retaliated by regularly shelling and bombing nearby towns.
In the town of Azaz, where the bombardment has become a near nightly occurrence, shells hit a family house overnight. Zeinab Hammadi said her two wounded daughters, aged 10 and 12, had been rushed across the border to Turkey, one with her brain exposed.
"We were sleeping and it just landed on us in the blink of an eye," she said, weeping as she surveyed the damage.
Family members tried to salvage possessions from the wreckage, men lifting out furniture and children carrying out their belongings in tubs.
"He (Assad) wants revenge against the people," said Abu Hassan, 33, working at a garage near the destroyed house. "What is the fault of the children? Are they the ones fighting?"
In addition to their tenuous grip on the north, the rebels also hold a crescent of suburbs on the edge of Damascus, which have come under bombardment by government forces that control the center of the capital.
Opposition activists said on Wednesday that dozens of people were incinerated in an inferno caused by an air strike on a petrol station in a Damascus suburb where residents were lining up for precious fuel.
The civil war in Syria has become the longest and bloodiest of the conflicts that rose out of uprisings across the Arab world in the past two years.
Assad's family has ruled for 42 years since his father seized power in a coup. The war pits rebels, mainly from the Sunni Muslim majority, against a government supported by members of Assad's Shiite-derived Alawite minority sect and some members of other minorities who fear revenge if he falls.
The West, most Sunni-ruled Arab states and Turkey have called for Assad to leave power. He is supported by Russia and Shiite Iran.
1 ) munir / gaza
03/01/2013 15:41
why is every article from reuters beirut sectarian ..btw forget fsa alqaida propganda..or journalism.but why are all the reporters based from beirut.. they all work for the hariris al-mustaqbal movmnt??
Note: Comments will be reviewed for appropriate content.
Click here for more details.
Arab leaders agree on joint military force
Joint military action, Yemen to dominate Arab summit
Syrian army turns to women on Damascus front lines
About us
Ma'an Images
Contact us
All Rights Reserved © Ma'an News Agency 2005 - 2014


text:
- Consumer Assistance
- Renew or Apply for a License
- Boards
- News/Media
- About Us
- DORA Home
We're redesigning the DORA website and we need your help. Please take our survey now!
At the present time, continuing education is not required to maintain or renew your license.
Adobe Acrobat Reader is required in order to view and print many of these materials. If you do not have Adobe Acrobat Reader, download the latest free version directly from the Adobe website, or convert a document to text.


text:
AD Tag – GNN
2AD – grey matter book ad
3Ad – caverns of blood
4AD – Brain Damage
Ad – Sam Hain
AD – Zoe Suckers Novel Ad
AD – level 33
New Ad – Wil Jackson
ad space
Ad – Scares that Care Weekend
Social Arena Banner
Subscribe and Follow
zergnet widget
News header
springboard widget – move reviews under
Film Reviews header
podcasts header
interviews header
Interview: Todd Lincoln - Director (V/H/S: Viral, The Apparition, Gorgeous Vortex)March 26th, 2015Interviewed by: Todd Martin
Exclusive Interview: Michael Berryman - Wednesday, March 25th at 9pm ESTMarch 23rd, 2015Interviewed by: Mike Joy
book reviews header
Book Review: The Pendle Curse - Author Catherine CavendishReviewed by: Dave Gammon
Book Review: Mutations - Authors E.L. Loraine and Tim FrasierReviewed by: Todd Martin
Film Review: Detention (2010)
SYNOPSIS: A  group of high school students are sent to Detention. Abandoned and locked in, images of Ghosts appear as they attempt to escape, only to find themselves connected to a past horrific death and made responsible for it with their lives. REVIEW: Director – James D.R. Hickox Starring – David Carradine, Billy Aaron Brown, Thomas Calabro Ah, the prank. Where would horror movies be without it? From Prom Night, Terror Train to Sorority Row, the … Continue reading


text:
When to start my Zinnias
I'm trying to figure out when to sow my Zinnias indoors. I start most of my seeds indoors (never did Zinnias yet) from February and on. I'm in Zone 5 and usually plant outside later in May to be safe.
I've read that starting Zinnias should be 3 to 4 weeks prior to my last frost date. It doesn't seem like they would be very tall to plant outside if germination takes a week or so. ??
What would you recommend I do? Sow them in March? April?
I've found that lately when I direct sow my Zinnia mixes, I don't get a good germination rate, bugs eat the new leaves, etc. So I was hoping by starting them inside I could actually have some Zinnias this year! :) They're my favorite annual flower and I'm trying a bunch of specific varieties, so I need a little help.
I have read a lot of the other posts, but there's a lot to sift through, and this was one question I still had that didn't get answered by reading them. :)
Thanks in advance.
This would probably be much too early to start zinnias indoors in Zone 5. I have actually had some zinnias start to bloom indoors in 5 weeks from their sowing date. I start my fancier zinnias indoors to get them beyond the cutworm susceptibility stage before I set them in the garden. I start those zinnias in 3-inch square pots. I plant one seed per pot or thin to one seedling per pot.
In two or three weeks I re-pot them in 5-inch square pots, where they can mature enough to be immune from cutworm damage.
I direct sow the majority of my zinnias and take some losses from cutworms, slugs, snails, and other critters. A Bt spray will get some of the small roving cutworms.
According to my records, I sow zinnias indoors under lights on 3/31.
I use diluted fish emulsion when they have their true leaves.
I grow large zinnias such as 'uproar rose' and 'giant wine'
and have them in quart size containers by the time they go outside end of May(hardened off,of course).
I grew two different Zahara zinnias last year and I think they were a good size when I put them in the ground.
Very helpful information. Thank you, both!
Zenman - You spiked my curiosity with your labeling system. What is the C6, TOM, PEP and the various numbers all mean? I log things in my books on when I sow my seeds and their germ. date, but I was curious as to how you organize/categorize your plantings/seedlings.
People think I'm so organized and experienced, but if they only saw and read what all of you do....wow! :)
Alicia, please note that zenman has a lighting system set up for his seedlings. This is important if you are going to grow quality plants inside.
I agree with the others....between 4 to 6 weeks is more than enough time to produce stocky little plants. (The smaller your seedling containers are, the sooner they will need to go into the ground.) They're fast little things, believe me.
I use cell packs and liner trays for the most part. I have some that are 18 to the sheet and others that are 24.
Yeah, I have the lights, heating mats and everything already. I grew about 100 different tomato plants last year and 50 'Red Elite' Geraniums and numerous other flowers/plants. :) Overall, they did well. I ended up giving a lot of the tomato plants away. I didn't think they'd do as well as they did. :)
The only thing I will do differently this year is not use the plugs in a couple of the seed-starting supplies I have. I did not like those at all. I got very long, stringy roots. I transplanted them, of course, once they got so big, but I felt they were pricey for what they produced as far as plants. I have pictures of the ones I started in dirt and the ones I started in the plugs, and the plugs had MUCH smaller plants. And they were the same type, same sow date, same light, etc. This year I'll use plain old seed-starting mix in the holes where the plugs go. And then I also use flats, cell packs, homemade newspaper pots and other misc. pots for starting seeds. I'm also buying two more stands with lights on each shelf this weekend, which will give me eight more shelves of growing!
I'll go off of the 4-6 weeks with the Zinnias, then. I just wanted to be certain I was going to have nice, sturdy plants to plant outside and wasn't sure of timing. You read so many things online, so it's always nice to see what others actually have done.
I do have a hardening off system also. :) I have a baker's rack outside that I took two clear shower liners and attached them together and place over it. Then I put my flowers in pots on the baker's rack for the summer. It works out well also. I just need to create something else until my husband builds me my small greenhouse. :)
How do you harden off your plants??
Thanks for your advice!
Inserting link to view a few pics I took last year of my seed-sowing and the long root on the one plug, etc. I didn't want to insert them in the post because my pics are too large for viewing that way.
Here is a link that might be useful: Seed-starting pics
"You spiked my curiosity with your labeling system. What is the C6, TOM, PEP and the various numbers all mean? I log things in my books on when I sow my seeds and their germ. date, but I was curious as to how you organize/categorize your plantings/seedlings."
Since I use small plastic labels, there isn't a lot of room on the label for a lengthy description, so I use short codes. I did a stint in the military, so I adopted the military/European convention for recording dates in the dd/mm/yy format, with just a "dot" separating the fields. TOM stands for tomato, PEP stands for pepper, and my zinnias get a letter-number code.
C6 was the 6th designated breeder specimen in the year "C", which was 2009. I actually started numbering my selected breeder zinnias in 2006 as 1,2,3,.. but by the end of the year realized that plain numbers were going to become unwieldy, so in 2007 I added the prefix "A" to all of my breeder codes in that year, with B for 2008, C for 2009, and D for 2010. All of my breeder specimens designated in 2011 will have a prefix of "E".
I keep a journal that records more detailed descriptions for each code. All of the seedlings with a C6 label were seeds from the specimen designated as C6. Only a few of those seedlings turned out well enough to be selected as breeders in 2010, with codes beginning with a "D". My journal entries make note of the female parent, so I have a maternal "blood-line" family tree for my zinnias. In many cases the male zinnia parent is not known. When it is, I make note of it in the journal.
In a truly scientific breeding program, both parents would be recorded to produce a complete genealogy, but even when I isolate a breeder seedhead with a "hairnet", I select pollen for it based on breeding objectives. For example, a spider flowered female would be pollinated with other spider flowered specimens or possibly with selfed pollen or possibly with interesting out-crosses like a good bi-colored specimen or some other likely-seeming cross.
Since zinnias are Composites, each petal is botanically a "flower" all by itself, and you could pollinate the stigma on each petal with different male pollen, if you were inclined to keep records at the petal-individual-seed level. Even if you were motivated to do that, it would be very difficult to devise a labeling system for individual zinnia petals. I do my zinnia breeding for fun, so I pretty much "take the path of least resistance".
ZM - I'm so impressed with all that you do! It truly inspires me. I would have to do a lot of research and ask a gazillion questions, but perhaps I will try to breed my own specimen someday. It sounds like you do quite well with that. And I really am quite serious in doing it because my brother and his wife just lost their baby before Christmas, and I would love to grow and name a flower after her, even if it's just for us to grow; not so much for registry. I looked into naming a flower after someone, and it's thousands of dollars, so forget that. :) But my family counts on me for growing them flowers, doing their landscaping, planting advice, so I thought that would be something very special that I could do for them.
Well, I appreciate all the guidance you've offered, and I prefer your labeling system over mine. :) I have a chart that I record things on that I print out each spring, and then I just label each pot or grouping, but it's hard to fit it all on the plant marker, so I find that your method would be much better.
Happy planting!


text:
Home Concert Tickets Rock and Pop Naughty Boy
Naughty Boy Tickets
Find the Naughty Boy tickets you're looking for on NME ticket exchange, the world's largest ticket marketplace. Select an event to view the range of tickets available or view the Naughty Boy tour on a map to find an event near you. You can also sell your spare Naughty Boy Concert Tickets on NME ticket exchange, and listing tickets is free. All Naughty Boy tickets are covered by the NME ticket exchange Ticket guarantee!


text:
Succulent roast also makes an excellent next-day soup
Chances are that the very first Easters were celebrated with lamb, since that was the staple meat in the Middle East at that time. But in North America, it's more likely we will be serving a ham with our family dinner.
Colic: Lots of theories, not many answersAbout a year ago, I was surprised to read that an elderly elephant at our local zoo had to be euthanized after a prolonged episode of colic.
Eyes front, towel on: Locker-room etiquette for the faint of heartExtend gym etiquette to locker-room
KitchenWise: Recipe for fresh and creamy asparagus soup with tarragonWhen shopping for asparagus, people often focus on the wrong thing — how thick the stalks are. They think thinner is better. Truth is, asparagus can be delicious regardless of how thick it is.
For Easter colour, crunch and flavour, look to the sideRecipes for Carrot-Fingerling Saute, Speedy Collard Ribbons, Quick-Roasted Beet and Carrot Jewels, Greek Potatoes, Orange Blossom Loukoumades
How soup becomes dinner and dinner becomes terrificRecipe for Ginger-Basil Turkey Meatball Soup
10 fresh ways to use maple syrup (that don't involve pancakes and waffles)Pancakes, waffles and French toast? They're fine, but they're also just the start.
Celebrate spring with an easy-to-assemble, delicious brunchRecipes for Sweet Miso 'Smoked' Salmon, Shaved Asparagus Salad, Breakfast Devilled Eggs, Roasted Squash and Apple Salad with Blood Orange Dressing
Mathematician reveals formula to find a perfect mateReject first 37per cent of candidates
Canadian maple syrup producers on tap for warmer days to get sap flowingGolden, amber, dark or very dark: Which maple syrup suits your taste?
Passover recipes combine tradition and fresh flavoursRecipes for Matzo Granola, Gefilte Fish in White Wine-Herb Broth, Red Wine and Honey Brisket, Passover Pear Cake
Function meets cool rebel fashion in fall-winter collection for RudsakToronto Fashion Week watch
Easter Dinner: Recipe for roasted ducks with citrus-tomato chutneyFor Easter dinner, how about trying something new, such as this duo of roasted ducks?


text:
Story Time Is Back - New Schedule and Time
Children's Story Time is back - with a new time and schedule. As we head through winter and into spring, Story Time will generally be happening two times a month on Wednesdays at 1:00 PM. Here's a list of upcoming dates:
Wednesday Mar 11 1:00 PM
Wednesday Mar 25 1:00 PM
Wednesday Apr 8 1:00 PM
Wednesday Apr 22 1:00 PM
We're looking forward to seeing you here!
Spanish Storytime Events
In addition to our regular Wednesday afternon story times, Carthage Public Library has received a grant to offer bilingual Spanish-English storytimes. Funding is provided through a Racing To Read LSTA grant from the Missouri State Library and the Institute of Museum and Library Services. These special children's story times are coordinated by Pat Wakefield. Here's a list of upcoming dates.
Tuesday Mar 10 9:00 AM
Tuesday Mar 17 9:00 AM
Tuesday Mar 24 9:00 AM
Tuesday Mar 31 9:00 AM
"Paws For Books" R.E.A.D. Dogs March Schedule
Our "Paws For Books" R.E.A.D. dogs will be visiting the Children's Area twice in March:
Wednesday afternoon, March 11, 2015, 1:00PM to 2:00PM (please note this last minute time change) Emmit & Truman with Sarah Saladin
Saturday morning, March 21, 2015, 10 AM 'till Noon - Merry with Peggy Potter
For more information, call 417-237-7040 during normal operating hours.
Teen Movie Day Featuring Mockingjay Part 1, Monday, March 16, 2015 2:00 PM - 4:00 PM
During Spring Break, Carthage Public Library will be having a special Teen Movie Day on Monday, March 16, 2015. We'll be showing the film Mockingjay Part 1 from 2:00 PM to 4:00 PM. This event is geared towards teens ages 13 to 17. Drinks and snacks will be provided. See you there!
Lots Of Changes, More Coming Soon
Carthage Public Library welcomes Sheila Denefrio, our new teen services person. You can find her in the teen area in the afternoons most weekdays, but you can reacher her anytime by sending firstname.lastname@example.com an email. We've completely rearranged to teen department upstairs with better seating and shelving. We're also rolling out new updated laptops for teens to use, and other technology. Lots more to come.
Join Us On Instagram
Check our our Instagram account at http://instagram.com/cplteenscene/


text:
Easy going,God fearing, originally from texas, i been in Atlanta for 4 years. I have 2 kids 1 boy and 1 girl that I love dearly, I'm pretty much an open book and will try anything as long as it's legal. I'm big on family and I would love to have that one woman to call my own. I've been on the dating scene and the pickings are slim so I'm ready for something real someone that can be a friend and possibly more and willing to advance in life mentally spiritually emotionally and physically
Join OkCupid
Find better matches with our advanced
Find better matches with our advanced
matching system
—% Match
—% Enemy
37 • Atlanta, GA • Man
I'm looking for
- Women
- Ages 18–38
- Located anywhere
- Who are single
- For new friends, long-term dating
My details
- Last online
- Yesterday – 7:13pm
- Orientation
- Straight
- Ethnicity
- Black
- Height
- 6′ 8″ (2.04m)
- Body type
- Athletic
- Diet
- —
- Smokes
- No
- Drinks
- Socially
- Drugs
- Never
- Religion
- Christianity
- Sign
- —
- Education
- —
- Job
- Sales / Marketing
- Income
- $40,000–$50,000
- Status
- Single
- Type
- Monogamous
- Offspring
- Has kids
- Pets
- —
- Speaks
- English
Similar Users
My self-summary
Write a little about yourself. Just a paragraph will do.
What I'm doing with my life
Don't overthink this one; tell us what you're doing day-to-day.
I'm really good at
Go on, brag a little (or a lot). We won't judge.
Telling the truth
The first things people usually notice about me
I'm an empty essay… fill me out!
My smile and my height
Favorite books, movies, shows, music, and food
Help your potential matches find common interests.
The six things I could never do without
Think outside the box. Sometimes the little things can say a lot.
I spend a lot of time thinking about
Global warming, lunch, or your next vacation… it's all fair game.
Making my next move my best move
On a typical Friday night I am
Netflix and takeout, or getting your party on — how do you let loose?
Depends on how I feel
The most private thing I'm willing to admit
I'm an empty essay… fill me out!
Get to know me and find out
You should message me if
Offer a few tips to help matches win you over.
You believe in organic chemistry and you know what you want
Connect your existing OkCupid account
Sign in
Welcome Back!
Restore your account to continue meeting new people.
Reset your password
We'll email you a link to reset it.
Email sent!
An email is on its way to . If you don't see it, try checking your Spam folder.


text:
Walnut Street Power/Lite Nightstand - WhiteItem # HN-FUB518
- Sturdy solid poplar frame
- Beautiful white finish
- Solid iron drawer pulls with a nickel finish
- Built-in power outlet and night-light
- Dimensions: 23 x 14.5W x 24H inches
Similar Kids Nightstands
Madison 3 Drawer Nightstand - Natural White
Lake House 2 Drawer Nightstand
Avenue 2 Drawer Nightstand
Schoolhouse 1 Drawer Nightstand - Pecan
Schoolhouse 1 Drawer Nightstand - White
Highlands 1 Drawer Nightstand
Reviewed by 1 customer
- Attractive Design
- Good Height
- Good Surface Space
- Sturdy
Best Uses
- Bedside Surface
Comments about Walnut Street Power/Lite Nightstand - White:
My wife and I shopped for awhile, this is very nice and arrived very quickly. Very happy with the purchae.
My Reward points
when you join
My Hayneedle Rewards
Write a review
for each review
for reviews including an
image or video
Customer Q & A
Enter your question, and one of our Customer Care experts will respond via email and also post the answer here.
Your Valid Email
Your Question


text:
Based on 5 reviews
Help make it easier for other Avvo users to choose the right lawyer by sharing your experience with this attorney. It's fast, simple, and safe.
Lawyers: Use the Peer Endorsements section to provide input about other attorneys.
While going through a DIVORCE I had worked with two other seperate divorce attorneys. They both showed frustration with the depth of my divorce and suggested I find another Attorney.
I then approached Mr. Hutterli with my situation. After he consulted with my prior attorney, Mr. Hutterli agreed to take my case.
Mr. Hutterli was then confronted by my wifes attorney, which my wife had chose a $400.oo P/H Attorney on Kruz Way in Lake Oswego and inturn runs his own law firm.
Mr. Hutterli's fees was barely over half of my wifes attorneys. Mr. Hutterly left no stones unturned and done the job succesfully to where I was able to settle my divorce with just a small fraction of the $100,000.00 that was being asked for by my wife of 6 and 1/2 years.
It is a pleasure to know a reasonably priced Attorney can KICK A--!! Through Mr. Hutterli's unfailing efforts he done just that! Thanks Conrad! (-:
As a client, Conrad was engaged in the the transfer of Real Estate involving multiple parties. Conrad's expertise identified the core issues involved with the transaction and how they could best be dealt with. With his assistance, the parties needs were satisfied and the transaction brought to a close.
I've worked with Conrad on a variety of items and every time I ask his opinion I'm impressed. He quickly sees through the BS and gives cogent, smart, easy advise. The kind of advise you want from a friend... and an attorney... not legal mumbo jumbo.
Conrad is a very professional attorney. He kept me informed through the whole process and I felt I could not be in better hands to go through my very long divorce. I would recommend him highly to anyone in need of an attorney.
Mr. Hutterli is a thoughtful, competent, and thorough lawyer. I very much appreciate how Mr. Hutterli made clear my rights and responsibilities thoughout the legal proceedings. His deep knowledge of marital law, and his thoughtful demeaner enabled me to go through this process and I learned much. I am particularly pleased at how clear he made particular aspects of marital law for me, and that his billing system is simple, clear, and accurately reflects his initial fee costs to me from the outset.


text:
Meet Your Deadlines: West Virginia AST Act Challenges
Schoenbrod: SCOTUS Ruling Helps EPA Deal With a "Stupid Statute"
Hot Topics for Waste-to-Energy Investors and Developers
Even assuming that the "significant nexus" test from Justice Kennedy's concurring opinion in Rapanos defines waters of the United States subject to Clean Water Act jurisdiction, the question remains what establishes a...more
In Sierra Club v ICG Hazard , LLC, 2015 WL 643382, the United States Court of Appeals for the Sixth Circuit held that a Kentucky coal mining company could not be sued by an environmental group for discharging selenium into...more
Environmental and Policy Focus: California lawmakers introduce sweeping new measures to increase renewable energy, cut fossil fuel use - San Jose Mercury News - Feb 10:
Last Tuesday, California lawmakers unveiled a...more
In 2008, EPA made a preliminary determination to use its residual designation authority (RDA) under the Clean Water Act to designate stormwater discharges from two or more acres of impervious surfaces in the Lower Charles...more
The Sixth Circuit recently ruled that facilities holding a Clean Water Act ("CWA") Section 402 general permit – one of two types of National Pollutant Discharge Elimination System ("NPDES") permits – may avail themselves of...more
There are a handful of legal and regulatory issues on the horizon in 2015 that are worth watching. Some of these issues, like the U.S. Environmental Protection Agency's (EPA's) Cooling Water Rule and the U.S. Army Corps of...more
"Waters of the United States" or "WOTUS" in the esoteric taxonomy of the Clean Water Act ("CWA"), is a term with which many are becoming increasingly familiar. This deceptively simple phrase is anything but simple in its...more
On Tuesday, Chief Judge Robert Chambers ruled that Fola Coal Company violated the Clean Water Act by discharging mine waste with sufficiently high levels of conductivity to cause or materially contribute to impairment of...more
Recently, the Wisconsin Supreme Court held that the pollution exclusion applies to well water contamination arising from the application of manure to nearby farmland, relieving a general liability insurer of its duty to...more
EPA Seeks Additional Comment On NPDES Electronic Reporting Rule: On December 1, 2014, the U.S. Environmental Protection Agency (EPA) requested additional comment on the proposed National Pollutant Discharge Elimination System...more
On April 21, 2014, the U.S. Environmental Protection Agency (EPA) and the U.S. Army Corps of Engineers (Corps) jointly released a new Proposed Rule – Definition of Waters of the U.S. Under the Clean Water Act – that would...more
It was inevitable I suppose. In a town where every policy discussion reaffirms the lampooning it receives in HBO's "Veep," it was only a matter of time until the EPA/Army Corps' proposed rule on the scope of jurisdiction...more
A landfill developer has asked the Supreme Court to review a decision of the Fifth Circuit holding that a jurisdictional determination by the U.S. Army Corps of Engineers is not final agency action subject to judicial review....more
Federal -
- Definition of "Waters of the United States" Under the Clean Water Act, 33 C.F.R. Part 328 -
- In connection with its proposed rule regarding the definition of...more
The Science Advisory Board has at last released its peer review of EPA's draft report on Connectivity of Streams and Wetlands to Downstream Waters: A Review and Synthesis, the technical support for the proposed rule on...more
Environmental and Policy Focus - Groups sue EPA over failure to reduce air pollution: KPCC - Oct 17:
Environmental groups have filed a lawsuit in U.S. District Court in San Francisco against the U.S. Environmental...more
The last thing that public agency leaders want to hear is that looming changes may make operating and building new public infrastructure more challenging and expensive. Unfortunately, proposed changes by the Environmental...more
The U.S. Environmental Protection Agency ("EPA") announced that it has extended the comment period on a proposed rule that has the potential to significantly expand the authority of the EPA and the U.S. Army Corps of...more
In This Issue:
- Food and Drug Administration Not Required to Ban Antibiotics Given to Food-Producing Animals
- U.S. Department of Agriculture Expands Crop Insurance under 2014 Farm Bill
Last year, the D.C. Circuit Court of Appeals ruled that EPA has authority to withdraw its approval for the specification of sites for the disposal of fill material, even after the Army Corps has issued a permit for the...more
While the Deepwater Horizon oil spill has largely disappeared from the news headlines, for the parties involved in the litigation, the legal machinations, particularly with respect to liability, have a long way still to...more
The Science Advisory Board has now provided its advice to EPA and the ACOE concerning their proposed rule clarifying the definition of "waters of the United States" under the Clean Water Act. In a brief letter that can only...more
This monthly publication provides information on compliance deadlines, regulatory developments, and other developments in federal, Maine, Massachusetts, and New Hampshire environmental and land use laws.
NEW REGULATORY...more
On September 3, 2014, the United States Court of Appeals for the Ninth Circuit issued its opinion in Alaska Community Action on Toxics v. Aurora Energy Services, LLC, holding that the Clean Water Act General Permit for...more
On September 16, 2014, the U.S. Environmental Protection Agency (EPA) released its Final 2012 Effluent Guidelines Program Plan (Final 2012 Plan) and the Preliminary 2014 Effluent Guidelines Program Plan (Preliminary 2014...more
Back to Top
Copyright ©


text:
accessibility & help
© Info
We provide short-term loans of wheelchairs and other equipment at almost 1,000 outlets in the UK.
Order a mobility aid from us.
The types of mobility aids we provide include:
order a mobility aid
You need to contact your local Red Cross office to find equipment near you. To see your office's contact details, simply type your postcode into our easy search tool.
This service is run by volunteers, so we do request donations to help us continue providing this valuable service. A refundable deposit may also be required.
Visit our independent living online shop or get in touch with your local Red Cross branch office to check whether they sell equipment.
We need your help to loan the right wheelchairs to people in crisis.
Donate now >
Helping people through difficult times
© British Red Cross 2015
All images © British Red Cross 2015
unless otherwise stated
British Red Cross, UK Office, 44 Moorfields, London EC2Y 9AL Phone: 0844 871 11 11. Fax: 020 7562 2000.
The British Red Cross Society, incorporated by Royal Charter 1908, is a charity registered in England and Wales (220949) and Scotland (SC037738)
Print this page


text:
valentine LLx Theme\Template by werksmannschaft valentine is a theme/template for Lightning Launcher. Please install either Lightning Launcher or Lightning Launcher eXt … 1,000+ downloads, Rating 4.26 (47) Free
Footplate2 by ZT.art Do your fingers react faster than your brain? Footplate will tell you the answer! It`s an easy game that everyone can p … 100,000+ downloads, Rating 4.18 (2434) Free
You Player Pro New by You Apps SoftDeV You Player Pro, la nueva generación de reproductores de video con características sociales comienza aquí! Estamos orgull … 100,000+ downloads, Rating 4.65 (21289) Free
Google Play Movies & TV by Google Inc. Google Play Movies & TV allows you to watch movies and TV shows purchased or rented on Google Play. Watch your favorite … 500,000,000+ downloads, Rating 3.63 (356897) Free
Alice Memo Color Write Note by kids photop Alice in Wonderland extremely popular by anime or comics. Alice which jumped out of anime Alice in Wonderland the simp … 1,000+ downloads, Rating 4.09 (11) Free
Hanuman Chalisa Story by Vegantaram The App for All Hanuman Bhakts. In the Hanuman Chalisa Story app read chalisa with hindi meaning and also available stor … 1,000+ downloads, Rating 4.65 (43) Free
¡Hola! - Learn Spanish by movin'App *** CREATED BY NATIVE SPANISH SPEAKERS *** Planning to visit Spain? Need a handy offline English-Spanish dictionary? Jus … 100,000+ downloads, Rating 4.02 (476) Free
FarmVille 2: Country Escape Updated by Zynga Escape to the world of farming, friends and fun! Go on farm adventures to collect rare goods and craft new recipes. Rais … 10,000,000+ downloads, Rating 4.17 (1223044) Free
O4Me – For you, Near You New by Marugal Labs Discover exciting offers & deals in shops & businesses near you, in your city.O4Me helps you discover shops you never kn … 10+ downloads, Rating 5.00 (3) Free
Gilmar Nascimento by Márcio Heli & Diogo Said Versão Mobile do App Gilmar Nascimento que reúne informações, projetos, fotos e uma área para você amigo falar conosco e … 10+ downloads, Rating 5.00 (3) Free
Winter Snowmobile 3D Simulator by Digital Toys Studio <b>Get behind wheels of extreme ATV vehicles enjoy climbing on frozen mountains and ice covered land in Winter Snowmobil … 50,000+ downloads, Rating 3.08 (838) Free
Chanakya Arthashastra by geekaap3 Kautilya's Arthashastra is an excellent treatise on statecraft, economic policy and military strategy. It is said to hav … 1,000+ downloads, Rating 4.43 (46) Free
Restaurant Status New by Pillion Inc. Restaurant Status is first cool application which lets you know your favourite restaurant status is now opened or closed … 5+ downloads, Rating 5.00 (3) Free
iCam Madrid by ipdroid Control the state of the roads with your smartphone or Android tablet in real time. See the roads index to locate the t … 10,000+ downloads, Rating 3.74 (78) Free
Examination of the heart by Mufeed Ali Alaradi The Cardiovascular examination is a portion of the physical examination that involves evaluation of the cardiovascular s … 1,000+ downloads, Rating 3.61 (28)


text:
CoAP Proxy Encode-To Option
|Document type:
|Expired Internet-Draft (individual)
|Document stream:
|No stream defined
|Last updated:
|2012-07-30 (latest revision 2012-01-24)
|Intended RFC status:
|Other versions:
|(expired, archived): plain text, pdf, html
|Stream State:
|No stream defined
|Document shepherd:
|No shepherd assigned
|IESG State:
|Responsible AD:
|Send notices to:
|No addresses provided
This Internet-Draft is no longer active. A copy of the expired Internet-Draft can be found here:
The scope of this document is to propose a new option to be used in conjunction with the CoAP proxy mechanisms. Several content types can be converted stateless into each other. The conversion may result in much more efficient representation of the resource. But currently no mechanism exists to indicate the proxy to perform the compression.
Guido Moritz <firstname.lastname@example.com>
(Note: The e-mail addresses provided for the authors of this Internet-Draft may no longer be valid)


text:
REF with door dispenser - to panel or not to panel?
We are planning a kitchen remodel and are wondering if you anyone has had experience with the water dispenser on refs with wood paneling. Does the water dripping (it always does at some point) ruin the wood over time? We are considering the GE monogram or Sub Zero. I like Kitchenaid too. I will never buy a VIking again because of past exp. I read bad things and good things about all of these brands. Price isn't an issue really, we just want a quality product. If we don't get the water dispenser (which my H really wants) what other option is there besides filling a pitcher all the time. Which I suppose I wouldn't have any problem with. I appreciate any comments!
My personal opinion is that if you are going to put cabinet grade paneling on a fridge, why would you ruin the look with an in door water dispenser?
There are options for that to be inside the fridge, if you must have it on the fridge.
Other options are a water dispenser at the sink, which to me is a much better option. Less problems with fridge units that don't have anything coming out of the door compared to those that do.
And any time you can buy things in a separate fashion you will also get better quality out of those items. Meaning if you buy a fridge with a water dispenser it is not as good as just a fridge or just a water dispenser, there are compromises that are always made when you combine any two or more items together.
My personal opinion, panels look best on appliances that are fully integrated to really look like part of the cabinetry. If you want an external ice/water dispenser - which I can totally understand, it was a requirement for us - then accept that the fridge is going to look like a fridge and don't bother dressing it up. It's really not that embarrassing, we've all got fridges!
If water is the only concern, then I'd do something at the sink like Phil suggests.


text:
LOS ANGELES (AP) — Two men charged with the severe beating of a San Francisco Giants fan at Dodger Stadium are suspected of assaulting three other Giants fans at the opening day game, a law enforcement official said Monday.
Detectives believe the other people were approached by suspects Louie Sanchez and Marvin Norwood on the west side of the stadium on March 31.
Eyewitnesses told police they saw Sanchez assault at least one of those unidentified men, said the official, who spoke on condition of anonymity because the investigation is ongoing.
Sanchez has been charged with misdemeanor assault in connection with that incident. Detectives believe both he and Norwood were involved in the other possible assaults, but no charges have been filed.
Sanchez also is accused of misdemeanor battery on a woman at the game. The official said the woman was wearing a Giants shirt and Sanchez threw something at her during the game.
Sanchez and Norwood were charged Friday with felony mayhem and assault charges in the beating of Bryan Stow, a paramedic from Santa Cruz who remains hospitalized with a brain injury.
Sanchez, 29, and Norwood, 30, made their first court appearance Monday. Their arraignment was continued until Aug. 10.
The Los Angeles Police Department has asked other possible victims to come forward, but made no further public statements Monday about the case.
The defendants were arrested Thursday in Rialto, about 55 miles east of Los Angeles. Their capture led to the exoneration of Giovanni Ramirez, a man police previously labeled as the prime suspect.
Court documents state that Norwood and Sanchez each inflicted great bodily injury on Stow, "causing him to become comatose due to brain injury and to suffer paralysis."
The mayhem count in the written complaint also alleged they "did cut and disable the tongue, and put out an eye," but district attorney spokeswoman Sandi Gibbons said that was legal language and that Stow had not had his eye or tongue taken out.
The case centers on incriminating statements the men have made, the official said.
Some people initially reported the suspects bragged about the incident to co-workers, though some are now backing off those statements, the official said.
Cell phone towers and photographs confirm that Norwood and Sanchez were at the game, the official said.
Attorney Gilbert Quinones, who represents Sanchez, acknowledged his client was at the stadium with his family but insisted he did not participate in the attack on Stow.
"He doesn't fit the profile of someone who would commit this type of crime," Quinones told reporters after his client appeared in court.
Quinones said he could not comment on the possibility of his client being involved in other assaults.
Norwood's public defender, Lee Rosen, made an unsuccessful request for his client's $500,000 bail to be reduced to $100,000. He did not immediately address the media.
Court documents state that police found five firearms, including an assault rifle, at Norwood's home. The document also states that Sanchez told witnesses not to provide information about the beating.
Both suspects were kept in Los Angeles police custody over the weekend. They appeared in court in their civilian clothes and not the jailhouse jumpsuits usually worn by prisoners.
Norwood is an apprentice carpenter, and Sanchez works at a car auction house in Fontana.
The attack on Stow reverberated throughout California and the nation as police and the Dodgers, whose financial woes have also brought national attention, sought to ease fears about violence at the storied stadium.
Stow, the father of two children, appeared to mouth his last name and might have tried to give a thumbs-up, according to a family blog post last week.
Court records show Norwood was sentenced in 2006 to three years' probation and served 118 days in jail after pleading guilty to one felony count of inflicting corporal injury on a spouse or cohabitant.
In 2003, Sanchez pleaded guilty to one felony count of inflicting corporal injury on a spouse or cohabitant, and the following year he pleaded no contest to one misdemeanor count of carrying a loaded firearm in a public place.
Sanchez is due back in court Aug. 1 for a bail hearing.
Watkins can be reached at www.twitter.com/thomaswatkins
Suspects in Stow Beating Accused of Other Assaults
Documents state that Norwood and Sanchez each inflicted great bodily injury on Stow
LOS ANGELES (AP) — Two men charged with the severe beating of a San Francisco Giants fan at Dodger Stadium are suspected of assaulting three other Giants fans at the opening day game, a law enforcement official said Monday.
Featured Careers & Jobs in EMS
Fire EMS Blogs
Blogger Browser
Today's Featured Posts
EMS Airway Clinic
Innovation & Advancement
This is the seventh year of the EMS 10 Innovators in EMS program, jointly sponsored by Physio-Control and JEMS.
More >
Press Conference, East Village Explosion and Collapse
Fire is contained to four buildings; 12 people have been injured.
Watch It >
D.C. Mayor Adds Ambulances to Peak Demand Period
10 additional ambulances will be on the streets from 11 a.m. to 11 p.m.
Watch It >
Utah Commission Privatizes Ambulance Service
Mayors in Iron County loose management fight.
Watch It >
Ambulance Delay Raises Concerns over Response Times
Officers give up after waiting 20 minutes for an ambulance.
Watch It >
Patient Carry during Snowstorm
Firefighters, medics and officers lend a hand in Halifax.
More >
Terror Attack in Tunisia
19 people killed outside of a museum.
More >
JEMS TV Product Innovations at EMS Today 2015: BoundTree CombiCarrier II
TheCombiCarrier II Backboard/Split Litter by Hartwell Medical
Watch It >
JEMS TV Product Innovations at EMS Today 2015: Knox MedVault
Controlled substance security
Watch It >
JEMS TV Product Innovations at EMS Today 2015: Hartwell CombiCarrier II
Versatile Spinal Immobilization Device
Watch It >
JEMS TV Product Innovations at EMS Today 2015: BoundTree Medstorm
Radiotransparent and radiotranslucent "one-pad system"
Watch It >