text: Home > Medical > Depression > Suicide Updated 23 June 2014 Suicide threats not just passing phase Suicidal tendencies in adolescents are often misinterpreted as a "passing phase" or common characteristics of adolescent development. Education about suicide can be life-saving. 0 Suicidal tendencies in adolescents are often misinterpreted as a "passing phase" or common characteristics of adolescent development. Some parents dismiss suicide threats as manipulative tactics, born out of immaturity. Education about suicide can be life saving. According to the South African Depression and Anxiety Group (SADAG) statistics suggest that only one in ten children who make suicide threats will carry them out. They warn, however, that it cannot be predicted who will, or under what circumstances these threats may actually lead to a suicide attempt. As a child gets older, suicide attempts become increasingly serious and at the same time, more likely to succeed. It is often difficult to recognise suicidal ideation. It is rare for children to discuss their suicidal thoughts with others. In those children who do not verbalise their self-destructive thoughts, suicidal tendencies are best recognised by marked mood and behavioural changes that may have been observed by family and friends. According to the SADAG, behavioural changes to look out for in troubled teens include: declining school performance extreme behaviour and mood change (persistent depression) loss of previous interests risk-taking behaviour drug or alcohol use social withdrawal a break in a key relationship From our sponsor Lack of sex contributes to depression in SA women SA's rising temperatures raise mental health concerns Read Health24's Comments Policy Comment on this story 0 comments Add your comment Thank you, your comment has been submitted. Logout Comment 0 characters remaining Share on Facebook Logout Comment 0 characters remaining Share on Facebook Loading comments... Ask the Expert Depression expert Michael Simpson has been a senior psychiatric academic, researcher, and Professor in several countries, having worked at London University in the UK; McMaster University in Canada; Temple University in Philadelphia, USA.; and the University of Natal in South Africa. Ask a question Questions posted to Depression expert questions answered by the expert Q: Nuzak: Is it possible that Nuzak could be the cause of my profound sweating on my head? posted on 30-03-2015 Q: i'm just so overwhelmed by life. Could this be why I am depressed? posted on 29-03-2015 Q: Is this combination of prescribed chronic medicines healthy? posted on 27-03-2015 Q: How effective is of valerian root? posted on 25-03-2015 Q: valerian root effectiveness posted on 25-03-2015 Q: I love kids but don't want to be touched posted on 23-03-2015 Q: Must i go for brain scan? posted on 22-03-2015 Q: If an individual is diagnosed with major depression, what effect domestic violence have on them? posted on 19-03-2015 Q: Can i use Nuzac together with Purata? I suffer heavy leg cramps and think this may be the reason? posted on 18-03-2015 Q: Should I seek help? posted on 17-03-2015 1 comment Q: what to try posted on 16-03-2015 1 comment Q: STRES posted on 16-03-2015 Q: Surgery and bad smoking habit posted on 15-03-2015 Q: will this feelings subside posted on 15-03-2015 Q: Can I use duromain with seredep ? posted on 12-03-2015 load more × Still have a question? Get free advice from our panel of experts Your question More detail Your name (optional) The information provided does not constitute a diagnosis of your condition. You should consult a medical practitioner or other appropriate health care professional for a physical exmanication, diagnosis and formal advice. Health24 and the expert accept no responsibility or liability for any damage or personal harm you may suffer resulting from making use of this content. I agree * You must accept our condition To prevent spam please solve this simple math problem: Forum Rules


text:
Skip the Line: Alhambra and Generalife Gardens Half-Day Tour
Read what other Viator.com travelers think about the Skip the Line: Alhambra and Generalife Gardens Half-Day Tour. What they loved, what they liked and what they think could be improved, it's all here to help you make the most of your next trip.
Click here for tour details, schedule and pricing
Great tour our guide knew a lot and expained evrything in detail. Was very helpful and Al hambra was a great experience. Should definitely take this tour. Highly recommended
This is the Must See when visiting Granada. You can't escape the views of this beautiful palace as you peruse around the city but you must do this tour to understand the splendor of this landmark. The tour is about 3 hours long and you should be in good shape to walk the entire time. Alberto was our tour guide and he was fantastic. We were visiting from the states and his English was impeccable and knowledge of the history unquestionable. We really enjoyed our time!
It's worth the money. Very convenient because You don't need to queue for tickets which is always a long queue. You may end up not getting one.
The tour guide was very knowledgeable and very thorough. We covered almost every part of Alhambra. My complain was that I was not picked up at my hotel.
Enrique L. was a great guide. The Alhambra and Generalife Gardens speak for themselves as a worthy destination. It is very crowded and difficult to book on your own, so I highly recommend this tour. It's worth every penny for the history, and ease of travel to and from the site.
This tour was amazing!
We loved our tour. Our tour guide was Antonio and he was fantastic. We will never forget him or the Alhambra. It was the best it could be.
So much to see. Expert information
This was a great way to see the Alhambra with English speaking guide explaining all we needed to know. No fighting with audio guides no intense swatting up weeks before. No queueing for tickets. A thoroughly professional tour through headphones and a walkie talkie type system
Well worth the money and so nice to be met/ picked up. Our guide Marco was very nice, knowledgable with the right blend of humour thrown in.
I would definitely recommend using this service, we would have missed so much detail if we had done this on our own.
Excellent tour. Guide spoke very well in two languages making us feel very at ease. Interesting place to visit and learn about its history.
We had a lovely time. Our tour guide, Esther was very knowledgable and friendly.
It was excellent and well worth the money. The guide was very knowledgeable. The only note I would add is that don't plan for them to start on time.
Our guide Marco is brilliant!
Tour was excellent. Our guide was very knowledgeable and fun.
The Alhambra is definitely worth seeing.
At night it's just spectacular.
Special Offer

subset: commoncrawl


text:
TagsView all tags
About unapproved sentences
You may see some sentences in red. These sentences are not approved by Tatoeba's community. They raise copyright issues or are otherwise problematic. If you are a contributor, please avoid translating them.
- date unknown
You will find this book very interesting.
- date unknown
linked to #219789
- sacredceltic
- Oct 22nd 2010, 19:21
linked to #580157
- paula_guisard
- Oct 14th 2011, 02:22
linked to #1174761
- soweli_Elepanto
- Aug 15th 2012, 22:01
linked to #1777143
- soweli_Elepanto
- Aug 15th 2012, 22:01
linked to #1777144
Report mistakes
Do not hesitate to post a comment if you see a mistake!
NOTE: If the sentence does not belong to anyone and you know how to correct the mistake, feel free to correct it without posting any comment. You will have to adopt the sentence before you can edit it.


text:
Survivor recalls Dachau, where SS terror began 80 years agotags: Nazi Germany, anniversaries, SS, Dachau, concentration camps
Max Mannheimer will never forget the words of his block leader when he entered the gates of Dachau concentration camp on 6 August 1944. "You're veterans at this by now," said the prisoner, a communist. "You know that the most important thing is not to draw attention to yourselves if you want to survive."
Behind Max, then aged 24, and his younger brother Edgar had lain a long and gruelling trudge through Nazi concentration camps, including Auschwitz and Theresienstadt, and the Warsaw ghetto, during which the siblings had lost their entire family, most of them in Auschwitz, simply for being Jewish.
In Dachau, Mannheimer was assigned the prisoner number 87098. "It was the last camp number I would ever have," the 93-year-old said. "But I took the block leader's message on board: 'You've got this far, just keep your head down, as the SS will pounce on you for the smallest violation'." He was liberated nine months later by US troops from a Dachau sub-camp, where one of his last jobs had been to cart the corpses of prisoners into the mortuary. Stricken with typhus, he had been reduced to skin and bones, weighing just 47kg. "I was a skeleton," he said. "I cried with both joy and despair."...
comments powered by Disqus
- Egyptian 'Mona Lisa' A Fake
- The Story Behind 'Woman in Gold': Nazi Art Thieves and One Painting's Return
- Scott Walker, Allergic to Dogs, May Run Against Political History
- Russian History Receives a Makeover That Starts With Ivan the Terrible
- Parsing Ronald Reagan's Words for Early Signs of Alzheimer's
- Joan Waugh on Grant's and Lee's 'gentlemen's agreement' ending the Civil War
- Charlatan or Sage? Contested Legacy of the late Dr. Ben, a Father of African Studies
- Historians make it easy for visitors to DC to understand the history of the Mall
- History's Grandin Wins Bancroft Prize for "The Empire of Necessity"
- Nobel prize-winning scientist writes a history of science


text:
OP Senior Member
Thanks Meter: 32
235 posts
Join Date:Joined: Aug 2011
i have a p500 and i am always changing roms and the last rom i have flashed recently is rc5 4.4 rom from jenkins. i don't remember but once i flashed a new rom a few month ago, i recognized that the proximity sensor does not work during calls. that means the screen does not go off in calls and my ear touches the screen and menus. so i searched a lot in internet and forums and found some solution for it but none of them fixed it. the last solution was in this link: http://forum.xda-developers.com/show....php?t=2353972 but the problem is that there is not such file sys/class/sensors... so what should i do? i have to mention that i have tested the proximity sensor with an app named androsensor and it shows that the proximity works well and detects my finger. so any suggestion or guide could be useful. please help me because this problem irritates me a lot.
Last edited by omidreza; 4th May 2014 at 12:25 PM.


text:
Call for Music Writers... Rock, Indie, Hip-hop, R&B, Electronic, Americana, Metal, World and More
At the outset, it looked like there was no hope for a kid hamlet, let alone a nation.
© 1999-2015 PopMatters.com. All rights reserved.PopMatters.com™ and PopMatters™ are trademarksof PopMatters Media, Inc.PopMatters is wholly independently owned and operated.


text:
Excerpts from Wendy's forthcoming book: Spiral Up!™
- Spiral Up! Introduction
- ENERGY: The 1st Co-Creative Power
- MIND: The 2nd Co-Creative Power
- FEELINGS: The 3rd Co-Creative Power
- SPEECH: The 4th Co-Creative Power
- INSPIRED ACTION: The 5th Co-Creative Power
Meet Wendy
Wendy Wallbridge, principal of On Your Mark Corporate Coaching & Consulting, Inc. is a pioneer in the coaching field. Recognized for her groundbreaking coaching techniques she has been interviewed by members of the print media including Fortune Magazine, the San Francisco Chronicle and the New York Times. In 1993 Wendy founded On Your Mark Corporate Coaching when she saw the need for individuals to do work that was both meaningful to them and met the changing needs in the marketplace. Today On Your Mark works with Fortune 100 leaders and teams seeking breakthrough outcomes and increased productivity in the face of significant, accelerating change. On Your Mark's clients have included Apple, Intel, Hewlett Packard, ABC-Disney, Wells Fargo, Oracle, Texas Instruments, Genentech, NetApp, VMware and McAfee Security, among others. Wendy is an intuitive coach … [Keep Reading...]
What Participants Are Saying About Spiral Up!™:
"taught me the skills to envision, name, and manifest meaningful work"
Wendy taught me the skills and tools to envision, name, and manifest meaningful work in the world work aligned with my spiritual values, and also in tune with the realities and the fun of tangible worldly, accomplishment and success. After working with Wendy in a group setting for 6 months, I see, speak, and act […]
More Testimonials...
- I realized that I could be the person I want to be
- I want all my friends and my sister to take Wendy's class
- I can claim my power instead of waiting to be chosen
- I can be successful without trying to be a man
- "a lifetime of old beliefs that needed reassessment!"


text:
In India, it has become easy to attack cultural artefacts: Salman Rushdie
- Ban Salman Rushdie at Jaipur Literature Festival, say Muslim groups, but Sanjay Roy says no 'hijacking' event
- Exclusive: At Idea Exchange, Pak High Commissioner Salman Bashir says UN mission played 'important role'
- Keep away controversial authors from Jaipur Literature Festival, demands Darul Uloom Deoband
Top Stories
- Supreme Court strikes down Section 66A, says it violates right to speech
- Pakistan Day: PM greets, MoS VK Singh tweets #disgust
- DK Ravi's death: Govt calls in CBI, tells court he had a 'relationship' with batchmate
- Mufti Mohammad Sayeed says will take Army into confidence on AFSPA
- 1987 Hashimpura massacre: The photographs that stand witness
"It is time we stop listening to people who didn't like the book, but to those who liked it. Books survive if enough people care about them,'' Rushdie said.
His relationship with India ''damaged'' because he wasn't allowed to visit the country for nine years, Rushdie is not surprised the movie will release in India. ''It is normal for films to release,'' he said. ''It will be a great moment when India will not feel the need for a censor board. What is wrong with the emergency? The Indian Express reported it,'' Rushdie said. Deepa Mehta added: ''There is nothing controversial about history.''
How did the collaboration go between the friends who worked together on the film Rushdie wrote the screenplay, Mehta directed it ''it is not always easy to work with friends, but we liked each other's work,'' was the common refrain.
Drawing on his bitter experiences at the Jaipur Literary Festival last year, which he was not allowed to attend because of protests from Muslims groups, Rushdie said: ''In India, it has become easy to attack cultural artifacts. People believe their identity is not defined by what you love, but by what you hate or are offended by.'' ''It is a spreading problem,'' he said.
And why does it happen? ''Because the people are apathetic and the state does not protect; the state should tell people some things are valuable. When it doesn't do this, smaller groups are allowed to get away with protests.''
''I was upset when India banned the Satanic Verses the first in the world to do so. Thankfully in this age you cannot ban books, because you can download them!''
''It is bad the fear of religious reprisal effecting freedom of speech,'' Rusdie said, while Mehta added: ''To me, the word 'controversy' is demeaning. My work is judged not on merit, but by what people think!'' ''People define their identity not by what the love, but by what they hate or are offended by,'' said Rushdie. ''In fact, I always thought that Satanic Verses was the least political of my books. Shame was directly confrontational.''
As both Rushdie and Mehta have looked down the barrel of public anger for their choice of topics, they have distinct views about how their work is received. Mehta I have never felt like a victim. Yes, there was anger and a touch of self pity at times.'' Rushdie was more forthright: ''People who want to limit free speech always speak about the dangers of speaking freely.'' He added: ''We live in an age of victim culture.''
Rushdie criticised the then Indian government for banning Satanic Verses: ''The ban was a moment of spinelessness, but it wasn't the only such moment. At the time of the ban, there wre no copies available in India!''
And India continues to influence their work. ''My stories have always come from India. India has broken my heart. The film Midnight's Children is my love letter to India,'' says Deepa Mehta.
Rushdie said: ''I was once worried that I was losing my grip over India. Midnight's Children was born from that need to reclaim. People loved this book and reclaimed me.''


text:
In the 50's I had to go to New York City to buy magic. There were very few stores, lousy instructions and no reviews to speak of. Today you can price...Read More ->
You know there are magic stores and then there are great magic stores. Well MJM Magic is even better than a great magic store. I use to shop over at...Read More ->


text:
Prepare and practice the speech because that is the best way to ease nervous tension. Find out about the audience, their backgrounds, requirements and concerns. Read the speech several times to be comfortable with the structure and the content. Remove big words and convoluted concepts because if you have trouble pronouncing or understanding something during practice, it can only get worse in front of a crowd.
How to Ease Nervous Tension While Giving a Speech
Lack of sleep, dry throat, sweaty hands, grasping the podium and fiddling with the tie are all signs of nervousness. According to a Toastmasters International manual on using gestures, you can control mental and emotional nervous energy through intense preparation and practice. Use gestures, eye contact and body movement to harness the physical nervous energy and channel it into a powerful presentation. You can never eliminate nervous tension, but you can control it and make those butterflies in your stomach fly in formation.
Visualize yourself giving the speech. Communication consultant and author Nick Morgan recommends that you create a detailed mental film of you giving the speech. Close your eyes and replay it in your mind. Visit the speech venue to help in this visualization.
Arrive early and mingle with the audience. This will ease some of the nervous tension for both you and your audience.
Stretch and breathe deeply just before the speech. Some speakers yawn and smile broadly before a speech because it relaxes the face muscles and works off some of the nervous energy. Some recommend imagining your audience in (or out of) various articles of clothing, but this might not always be a relaxing experience. Take small sips of water, but avoid ice-cold water because that could dry out your throat.
Memorize the first minute of your speech. Even if you are reading from your notes the rest of the way, commit the first 100 words to memory. Establish eye contact with the audience and smile. Use humor sparingly in the introduction because the audience might not share your sense of humor.
Use natural gestures. Do not try to memorize hand and body movements because you might look stilted. Body movement burns nervous energy but over-gesturing could distract the audience. Do not take halting steps, shuffle or rock on your feet because these are outward signs of nervousness. Everything you do on stage must have a purpose.
Focus on your audience. Stop worrying about how you look and do not over-analyze audience reaction. Do not let distractions affect you because you could lose your train of thought and ruin the rest of your speech. If you miss a word or even a paragraph, do not apologize and start over because the chances are your audience never caught it.
Relax because your audience wants you to succeed. Imagine your listeners secretly cheering for you during the speech and applauding you at the end of the speech.
- 1
- Photo Credit Jupiterimages/Goodshoot/Getty Images
You May Also Like
How to Not Get Nervous When Giving a Speech
Some people become very anxious at the thought of public speaking. Standing in front of a large crowd of spectators can be...
Tips on Not Getting Nervous During a Presentation
How to Not Be Nervous During a Presentation. For most people, ... Delivering a speech or presentation can be daunting to the...
How to Not Be Nervous During a Presentation
How to Not Be Nervous When Singing. Stage fright is a very real emotion for entertainers. ... Avoid items and topics that...
How to To Tell A Joke Before A Public Speech
How to To Tell A Joke Before A Public Speech. Public speaking is a source of great anxiety, or even fear, ......
How to Give an Inspiring Speech
The prospect of giving a speech can make even the most experienced speech writer nervous. Nonetheless, speeches can be powerful motivational tools...
How to Stop Blushing When Presenting
How to Give a Presentation Without Being Nervous. Just the thought of having to give a presentation for a class, ... How...
How to Overcome Anxiety in Public Speaking
Proven exercises can help to ease your public speaking stress and tension. ... often experiences symptoms such as shaking ... to overcome...
How to Ease Anxiety
Anxiety is one of the more common conditions for many Americans. Typical symptoms include a shortness of breath, a fast pulse rate...
How to Give a Speech
How to Give a Speech. Public speaking does not come easily to everyone. ... Remember, most people get nervous before going in...
Why Do People Get Nervous When Speaking in Public?
Strategies for overcoming fear of public speaking include practicing your speech in front of family members or friends and mentally trying to...
Good Presentations: What to Do When Nervous
How to Give a Good Presentation. Good Presentations: Personal Style 2. ... The question is not what to do if you're nervous...


text:
Record: 2-5-1, Finished 11th in APFA
APFA Season Summary
Scored 49 points (6.1/g), 10th of 14 in the APFA.
Allowed 63 points (7.9/g), 10th.
Differential of -14 points (-1.8/g), 9th.
Expected W-L: 2.8-5.2.
Coached by
Guil Falcon
Our primary support comes from users like you. Sponsor this page for $5. Your message will replace this ad.


text:
Are you Jongsun Kim?Claim your profile
Publications (37)121.46 Total impact
Article: Protection of Mice against Pandemic H1N1 Influenza Virus Challenge after Immunization with Baculovirus-expressed Stabilizing Peptide fusion Hemagglutinin protein.[Show abstract] [Hide abstract]
ABSTRACT: Current influenza vaccines are produced in embryonated chicken eggs. However, egg-based vaccines have various problems. To address these problems, recombinant protein vaccines have been developed as new vaccine candidates. Unfortunately, recombinant proteins frequently encounter aggregation and low stability during their biogenesis. It has been previously demonstrated that recombinantly expressed proteins can be greatly stabilized with high solubility by fusing stabilizing peptide (SP) derived from the c-terminal acidic tail of human synuclein (ATS). To investigate whether SP fusion proteins can induce protective immunity in mice, we produced influenza HA and SP fusion protein using baculovirus expression system. In in vitro tests, SP-fused recombinant HA1 (SP-rHA1) was shown to be more stable than recombinant HA1 (rHA1). Mice were immunized intramuscularly with baculovirus-expressed rHA1 protein or SP-rHA1 protein (2ug/mouse) formulated with aluminum hydroxide. Antibody responses were determined by ELISA and hemagglutination inhibition assay. We observed that SP-rHA1 immunization elicited HA-specific antibody response that was comparable to rHA1 immunization. These results indicate that fusion of SP to rHA1 does not negatively affect the immunogenicity of the vaccine candidate. Therefore, it is possible to apply SP fusion technology to develop stable recombinant protein vaccines with high solubility.
Article: A Comparative Study of the Effects of Inhibitory Cytokines on Human Natural Killer Cells and the Mechanistic Features of Transforming growth factor-beta[Show abstract] [Hide abstract]
ABSTRACT: The major factors and mechanisms by which natural killer (NK) cells are inhibited in cancer patients have not yet been well defined. In this study, we conducted a comparative analysis of the effects of TGF- β, IL-10, and IL-4 on primary NK cells, and it was demonstrated that (1) TGF-β most potently inhibited the overall function of NK cells. (2) It appears that TGF-β reduced the tyrosine phosphorylation of Syk and the expression of c-myc. (3) It was also found that the IL-2-induced promoter-binding activities of C-myb, AP-1, CREB, and AR were also completely suppressed upon TGF-β treatment. Interestingly, TGF-β also completely suppressed other transcription factors, which are constitutively activated. Among these factors, we further confirmed roles of AP-1 in NK-92 cell activation through c-jun and MEK1 inhibitor assay. Our study provides insight into the effects of TGF-β in modulating NK cell functions.Cellular Immunology 07/2014; DOI:10.1016/j.cellimm.2014.05.001 · 1.87 Impact Factor
[Show abstract] [Hide abstract]
ABSTRACT: Natural killer (NK) cells are lymphocytes of the innate immune system and have the ability to kill tumor cells and virus-infected cells without prior sensitization. Malignant tumors and viruses have developed, however, strategies to suppress NK cells to escape from their responses. Thus, the evaluation of NK cell activity (NKA) could be invaluable to estimate the status and the outcome of cancers, viral infections, and immune-mediated diseases. Established methods that measure NKA, such as (51)Cr release assay and CD107a degranulation assay, may be used to determine NK cell function, but they are complicated and time-consuming because they require isolation of peripheral blood mononuclear cells (PBMC) or NK cells. In some cases these assays require hazardous material such as radioactive isotopes. To overcome these difficulties, we developed a simple assay that uses whole blood instead of PBMC or isolated NK cells. This novel assay is suitable for high-throughput screening and the monitoring of diseases, because it employs serum of ex vivo stimulated whole blood to detect interferon (IFN)-γ secreted from NK cells as an indicator of NKA. After the stimulation of NK cells, the determination of IFNγ concentration in serum samples by enzyme-linked immunosorbent assay (ELISA) provided a swift, uncomplicated, and high-throughput assay of NKA ex vivo. The NKA results microsatellite stable (MSS) colorectal cancer patients was showed significantly lower NKA, 263.6±54.5pg/mL compared with healthy subjects, 867.5±50.2pg/mL (p value <0.0001). Therefore, the NKA could be utilized as a supportive diagnostic marker for microsatellite stable (MSS) colorectal cancer.Biochemical and Biophysical Research Communications 03/2014; DOI:10.1016/j.bbrc.2014.02.040 · 2.28 Impact Factor
Article: Susceptibility of CD24(+) ovarian cancer cells to anti-cancer drugs and natural killer cells[Show abstract] [Hide abstract]
ABSTRACT: Natural killer cells are lymphocytes of the innate immune system that play a key role in the direct elimination of transformed or virus-infected cells. Recently, it has been reported that NK cells can attack cancer cells with stem cell-like properties. In this study, we isolated ovarian cancer cell lines CAOV3 and TOV21G with and without CD24, which has been reported as an ovarian cancer stem cell marker, and compared their drug resistance and susceptibility to NK cell lysis. The isolated CD24(+) CAOV3 and TOV21G cells were more resistant to cisplatin and doxorubicin anti-cancer drugs. Also, CD24(+) CAOV3 and TOV21G cells were more susceptible to NK cell lysis compared with CD24(-) cells. In order to identify reasons for the differing NK cell susceptibility, we examined NK cell-killing mechanisms against CD24(+) cancer cell lines by analyzing NKG2D ligands, MHC class I molecules, and natural cytotoxic receptor ligands expression on target cells. Consistently, CD24(+) CAOV3 and TOV21G cells showed up-regulated NKG2D ligands and down-regulated MHC class I molecule expression. These findings show that CD24(+) ovarian cancer cell lines are more resistant to antitumor drugs but are more susceptible to NK cell lysis; thus, NK cell immunotherapy might be useful in eliminating ovarian cancer stem cells and preventing tumor recurrence and metastasis.Biochemical and Biophysical Research Communications 09/2012; 427(2):373-8. DOI:10.1016/j.bbrc.2012.09.067 · 2.28 Impact Factor
Article: The chaperone activity of α-synuclein: Utilizing deletion mutants to map its interaction with target proteins[Show abstract] [Hide abstract]
ABSTRACT: α-Synuclein is the principal component of the Lewy body deposits that are characteristic of Parkinson's disease. In vivo, and under physiological conditions in vitro, α-synuclein aggregates to form amyloid fibrils, a process that is likely to be associated with the development of Parkinson's disease. α-Synuclein also possesses chaperone activity to prevent the precipitation of amorphously aggregating target proteins, as demonstrated in vitro. α-Synuclein is an intrinsically disordered (i.e., unstructured) protein of 140 amino acids in length, and therefore studies on its fragments can be correlated directly to the functional role of these regions in the intact protein. In this study, the fragment containing residues 61-140 [α-syn(61-140)] was observed to be highly amyloidogenic and was as effective a chaperone in vitro as the full-length protein, while the N- and C-terminal fragments α-syn(1-60) and α-syn(96-140) had no intrinsic chaperone activity. Interestingly, full-length fibrillar α-synuclein had greater chaperone activity than nonfibrillar α-synuclein. It is concluded that the amyloidogenic NAC region (residues 61-95) contains the chaperone-binding site which is optimized for target protein binding as a result of its β-sheet formation and/or ordered aggregation by α-synuclein. On the other hand, the first 60 residues of α-synuclein modulate the protein's chaperone-active site, while at the same time protecting α-synuclein from fibrillation. On its own, however, this fragment [α-syn(1-60)] had a tendency to aggregate amorphously. As a result of this study, the functional roles of the various regions of α-synuclein in its chaperone activity have been delineated.Proteins Structure Function and Bioinformatics 05/2012; 80(5):1316-25. DOI:10.1002/prot.24028 · 3.34 Impact Factor
Article: Expression Patterns of α-Synuclein in Human Hematopoietic Cells and in Drosophila at Different Developmental Stages[Show abstract] [Hide abstract]
ABSTRACT: α-Synuclein, a presynaptic protein of the central nervous system, has been implicated in the synaptic events such as neuronal plasticity during development and learning, and neuronal degeneration under pathological conditions. As an effort to understand the biological function of α-synuclein, we examined the expression patterns of α-synuclein in various human hematopoietic cells, and in Drosophila at different developmental stages. The α-synuclein was ubiquitously expressed in all the tested hematopoietic cells including T cells, B cells, NK cells, and monocytes, as well as in the lymphoma cell lines, Jurkat and K562. A potential α-synuclein homologue was also expressed in Drosophila, and its expression appeared to be temporally and spatially regulated during development. Our data suggest that α-synuclein may function in invertebrates as well as in vertebrates and its function may not be restricted to the neuron. KeywordsDevelopment-Gene Expression-NACP-Neurodegenerative Disease-Synuclein-SynucleinMolecules and Cells 04/2012; 10(1):65-70. DOI:10.1007/s10059-000-0065-x · 2.24 Impact Factor
[Show abstract] [Hide abstract]
ABSTRACT: Although previous studies on hexokinase (HK) II indicate both the N- and C-terminal halves are catalytically active, we show in this study the N-terminal half is significantly more catalytic than the C-terminal half in addition to having a significantly higher Km for ATP and Glu. Furthermore, truncated forms of intact HK II lacking its first N-terminal 18 amino acids (delta18) and a truncated N-terminal half lacking its first 18 amino acids (delta18N) have higher catalytic activity than other mutants tested. Similar results were obtained by PET-scan analysis using (18)FFDG. Our results collectively suggest that each domain of HK II possesses enzyme activity, unlike HK I, with the N-terminal half showing higher enzyme activity than the C-terminal half.BMB reports 07/2009; 42(6):350-5. DOI:10.5483/BMBRep.2009.42.6.350 · 1.99 Impact Factor
Article: On the mechanism of internalization of alpha-synuclein into microglia: roles of ganglioside GM1 and lipid raft.[Show abstract] [Hide abstract]
ABSTRACT: ALpha-synuclein (alpha-syn) has been known to be a key player of the pathogenesis of Parkinson's disease and has recently been detected in extracellular biological fluids and shown to be rapidly secreted from cells. The penetration of alpha-syn into cells has also been observed. In this study, we observed that dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol, a glucosyltransferase inhibitor, and proteinase K inhibited the internalization of extracellular monomeric alpha-syn into BV-2 cells, and the addition of monosialoganglioside GM1 ameliorated the inhibition of alpha-syn internalization in dl-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol-treated BV-2 cells. Furthermore, inhibition of clathrin-, caveolae-, and dynamin-dependent endocytosis did not prevent the internalization of alpha-syn, but disruption of lipid raft inhibited it. Inhibition of macropinocytosis and disruption of actin and microtubule structures also did not inhibit the internalization of alpha-syn. In addition, we further confirmed these observations by co-culture system of BV-2 cells and alpha-syn-over-expressing SH-SY5Y cells. These findings suggest that extracellular alpha-syn is internalized into microglia via GM1 as well as hitherto-unknown protein receptors in clathrin-, caveolae-, and dynamin-independent, but lipid raft-dependent manner. Elucidation of the mechanism involved in internalization of alpha-syn should be greatly helpful in the development of new treatments of alpha-syn-related neurodegenerative diseases.Journal of Neurochemistry 06/2009; 110(1):400-11. DOI:10.1111/j.1471-4159.2009.06150.x · 4.24 Impact Factor
[Show abstract] [Hide abstract]
ABSTRACT: Natural cytotoxicity receptors (NCRs) are major activating receptors involved in NK cytotoxicity. NCR expression varies with the activation state of NK cells, and the expression level correlates with NK cells' natural cytotoxicity. In this study, we found that Gö6983, a PKC inhibitor, induced a remarkable increase of NCR expression on primary NK cells, but other PKC inhibitors and NK cell stimulators such as IL-2 and PMA, did not. Gö6983 increased the expression of NCR in a time- and concentration-dependent manner. Furthermore, Gö6983 strongly upregulated the surface expression of death ligands FasL and TRAIL, but not cytotoxic molecules perforin and granzyme B. Unlike two other NK stimulating molecules, IL-2, and PMA, Gö6983 did not induce NK cell proliferation. Up-regulation of NCRs and death ligands on NK cells by Gö6983 resulted in a significant enhancement of NK cytotoxicity against various cancer cell lines. Most importantly, administration of Gö6983 effectively inhibited pulmonary tumor metastasis in mice in a dose-dependent manner. These results suggest that Gö6983 functions as an NK cell activating molecule (NKAM); this NKAM is a novel anti-cancer and anti-metastasis drug candidate because it enhances NK cytotoxicity against cancer cells in vivo as well as in vitro.Cancer Immunology and Immunotherapy 04/2009; 58(10):1691-700. DOI:10.1007/s00262-009-0680-0 · 3.64 Impact Factor
Article: Identification of the amino acid sequence motif of alpha-synuclein responsible for macrophage activation.[Show abstract] [Hide abstract]
ABSTRACT: Alpha-synuclein (Syn) is implicated in the pathogenesis of PD and related neurodegenerative disorders. Recent studies have also shown that alpha-synuclein can activate microglia and enhance dopaminergic neurodegeneration. The mechanisms of microglia activation by alpha-synuclein, however, are not well understood. In this study, we found that not only alpha-synuclein but also beta- and gamma-synucleins activated macrophages (RAW 264.7) in vitro. Macrophages treated with synuclein proteins secreted TNF-alpha in a dose-dependent manner. Synuclein family proteins also increased mRNA transcription of COX-2 and iNOS. Two alpha-synuclein deletion mutants, SynDeltaNAC and Syn61-140, activated macrophages, while deletion mutants Syn1-60 and Syn96-140 did not significantly activate them. Finally, we demonstrated that macrophage activation by alpha-synuclein was accompanied by phosphorylation of ERK. These results suggest that synuclein family proteins can activate macrophages, and that macrophage activation needs both the N-terminal and C-terminal domains of alpha-synuclein, but not the central NAC region.Biochemical and Biophysical Research Communications 04/2009; 381(1):39-43. DOI:10.1016/j.bbrc.2009.02.002 · 2.28 Impact Factor
Article: Expression of human interferon alpha-1 with enhanced stability via the tagging system of a stabilizing peptide.[Show abstract] [Hide abstract]
ABSTRACT: Human interferon alpha-1 (hIFNA1) is one of several interferon alpha subtypes that have been studied and commercialized to treat various viral diseases including hepatitis B and C as well as malignant melanoma. Protein aggregation has been problematic for every step in commercial production, from purification to the packaging and delivery of pharmaceutical proteins. In a previous study, we demonstrated that a stabilizing peptide from the C-terminal acidic tail of alpha-synuclein (ATS) could be used as an effective fusion tag to increase the stability of target proteins such as human growth hormone (hGH) and granulocyte colony-stimulating factor (G-CSF). In this study, we applied this ATS fusion system to hIFNA1 in order to protect against the aggregation of hIFNA1 by environmental stresses, since hIFNA1 aggregates elicit an undesirable immune response in humans. As expected, ATS-fused hIFNA1 (hIFNA1-ATS) protein showed enhanced stability against thermal stress, agitation stress, and repetitive freeze/thawing stress in comparison with native hIFNA1. More importantly, hIFNA1-ATS fusion protein appeared to be 1.6 times more active than hIFNA1 in a cell anti-proliferation assay. Furthermore, the solubility of hIFNA1-ATS appeared to be 1.7 times higher than that of native protein. Our results suggest that the ATS-tag system could be a useful means for protecting hIFNA1 protein from aggregation by various external stresses and could be used to increase the solubility of protein.Protein Expression and Purification 11/2008; 63(2):140-6. DOI:10.1016/j.pep.2008.09.016 · 1.43 Impact Factor
[Show abstract] [Hide abstract]
ABSTRACT: alpha-Synuclein is the major amyloidogenic component observed in the Lewy bodies of Parkinson's disease. Amyloid fibrils of alpha-synuclein prepared in vitro were instantaneously disintegrated by dequalinium (DQ). Double-headed cationic amphipathic structure of DQ with two aminoquinaldinium rings at both ends turned out to be crucial to exert the disintegration activity. The defibrillation activity was shown to be selective toward the fibrils of alpha-synuclein and Abeta40 while the other beta2-microglobulin amyloid fibrils were not susceptible so much. Besides the common cross beta-sheet conformation of amyloid fibrils, therefore, additional specific molecular interactions with the target amyloidogenic proteins have been expected to be involved for DQ to exhibit its defibrillation activity. The disintegrating activity of DQ was also evaluated in vivo with the yeast system overexpressing alpha-synuclein-GFP. With the DQ treatment, the intracellular green inclusions turned into green smears, which resulted in the enhanced cell death. Based on the data, the previous observation that DQ led to the predominant protofibril formation of alpha-synuclein could be explained by the dual function of DQ showing both the facilitated self-oligomerization of alpha-synuclein and the instantaneous defibrillation of its amyloid fibrils. In addition, amyloidosis-related cytotoxicity has been demonstrated to be amplified by the fragmentation of mature amyloid fibrils by DQ.Biochimica et Biophysica Acta 11/2008; 1780(10):1156-61. DOI:10.1016/j.bbagen.2008.07.006 · 4.66 Impact Factor
Article: Down syndrome critical region 2 protein inhibits the transcriptional activity of peroxisome proliferator-activated receptor beta in HEK293 cells[Show abstract] [Hide abstract]
ABSTRACT: Down syndrome is mainly caused by a trisomy of chromosome 21. The Down syndrome critical region 2 (DSCR2) gene is located within a part of chromosome 21, the Down syndrome critical region (DSCR). To investigate the function of DSCR2, we sought to identify DSCR2-interacting proteins using yeast two-hybrid assays. A human fetal brain cDNA library was screened, and DSCR2 was found to interact with a member of the nuclear receptor superfamily, peroxisome proliferator-activated receptor beta, (PPARbeta). A co-immunoprecipitation assay demonstrated that DSCR2 physically interacts with PPARbeta in mammalian HEK293 cells. DSCR2 also inhibited the ligand-induced transcriptional activity of PPARbeta. Furthermore, PPARbeta also decreased the solubility of DSCR2, which increased levels of insoluble DSCR2.Biochemical and Biophysical Research Communications 10/2008; 376(3):478-82. DOI:10.1016/j.bbrc.2008.09.017 · 2.28 Impact Factor
Article: Amino-acid sequence motifs for PKC-mediated membrane trafficking of the inhibitory killer Ig-like receptor.[Show abstract] [Hide abstract]
ABSTRACT: Activation-induced upregulation of inhibitory killer Ig-like receptor (KIR) is regulated by protein kinase Cs (PKCs). Conventional PKCs increase KIR expression on the post-transcriptional level by increasing the recycling of surface molecules and endoplasmic reticulum (ER)-Golgi processing. PKCdelta plays a role in the secretion of cytoplasmic KIR through lytic granules. In this study, we identified amino acid sequence motifs associated with PKC-mediated KIR membrane trafficking by systematic mutagenesis. Mutations of Y(398) and HLWC(364) completely inhibited the PMA-induced increase of KIR molecules at surface as well as total protein levels, indicating that these are associated with ER-Golgi processing and sorting to plasma membrane through lytic granules. Mutations of Y-based motif, including Y(398), acidic region (PE(394)), dileucine motif-like region (IL(423)) and PKC-phosphorylatable S(415) caused a blockade of surface KIR endocytosis after PKC stimulation. Mutation of T(145) caused an accumulation of mutant proteins in late endosomes and lysosomes after PKC activation, suggesting that T(145) might be related to the recovery of endocytosed KIR to the surface membrane. We also demonstrated that PKCs could directly phosphorylate the KIR cytoplasmic tail by means of western blot and in vitro kinase assay, implying that phosphorylation status of KIR cytoplasmic tail can direct the fate of surface KIR molecules. Taken together, various sequence motifs are implicated in the PKC-mediated post-transcriptional upregulation of KIR, and each of these motifs work in different steps after PKC activation.Immunology and Cell Biology 02/2008; 86(4):372-80. DOI:10.1038/icb.2008.5 · 4.21 Impact Factor
Article: A soluble and heat-resistant form of hantavirus nucleocapsid protein for the serodiagnosis of HFRS[Show abstract] [Hide abstract]
ABSTRACT: Hemorrhagic fever with renal syndrome (HFRS) is a rodent-borne viral zoonosis characterized by fever, hemorrhagic manifestations, and renal disorder. The causative agent of HFRS has been identified as a hantavirus. Hantavirus nucleocapsid proteins have recently been shown to be immunodominant antigens in HFRS, inducing an early and long-lasting immune response, and their amino termini are sensitive tools for the detection of specific antibodies in HFRS patient sera. Previous work has demonstrated that the introduction of the acidic tail of alpha-synuclein (ATS) into heat-labile proteins protects them from heat-induced aggregation. In this study, the ATS peptide was introduced into the N-terminal antigenic portion of the nucleocapsid proteins (amino acid residues 1-70) of the Hantaan virus (HTNV-delta N) and Seoul virus (SEOV-delta N). The recombinant ATS-HTNV-delta N and ATS-SEOV-delta N fusion proteins were heat-resistant, and the proteins purified by heat treatment were immunoreactive to sera from patients with HFRS. Compared with sera from patients with leptospirosis and scrub typhus, sera from patients with HFRS showed much higher reactivity in ATS-HTNV-delta N- or ATS-SEOV-delta N-based IgG ELISAs. Immunoblotting analysis revealed that only sera from patients with HFRS specifically recognized the ATS-HTNV-delta N and ATS-SEOV-delta N, indicating that the ATS-HTNV-delta N and ATS-SEOV-delta N were highly purified species without any other immunoreactive proteins as contaminants. These data demonstrate that the ATS-HTNV-delta N and ATS-SEOV-delta N fusion proteins offer a safe and inexpensive source of pure and specific antigen for large-scale diagnosis and seroepidemiological studies of HFRS, and that ATS-fusion technology can also be utilized to solubilize other antigens that could be used for large-scale diagnosis and seroepidemiological studies of other diseases.Journal of Virological Methods 02/2008; 147(1):1-9. DOI:10.1016/j.jviromet.2007.08.001 · 1.88 Impact Factor
Article: Crystallization and preliminary crystallographic analysis of the fourth FAS1 domain of human BigH3.[Show abstract] [Hide abstract]
ABSTRACT: The protein BigH3 is a cell-adhesion molecule induced by transforming growth factor-beta (TGF-beta). It consists of four homologous repeat domains known as FAS1 domains; mutations in these domains have been linked to corneal dystrophy. The fourth FAS1 domain was expressed in Escherichia coli B834 (DE3) (a methionine auxotroph) and purified by DEAE anion-exchange and gel-filtration chromatography. The FAS1 domain was crystallized using the vapour-diffusion method. A SAD diffraction data set was collected to a resolution of 2.5 A at 100 K. The crystal belonged to space group P6(1) or P6(5) and had two molecules per asymmetric unit, with unit-cell parameters a = b = 62.93, c = 143.27 A, alpha = beta = 90.0, gamma = 120.0 degrees.Acta Crystallographica Section F Structural Biology and Crystallization Communications 11/2007; 63(Pt 10):893-5. DOI:10.1107/S1744309107039358 · 0.57 Impact Factor
Article: Activation-induced upregulation of inhibitory killer Ig-like receptors is regulated by protein kinase C.[Show abstract] [Hide abstract]
ABSTRACT: Inhibitory killer Ig-like receptor (KIR) expression was upregulated by protein kinase C (PKC) activation in stable Jurkat clones that express KIR or CD8KIR fusion proteins. PKC-induced KIR upregulation was mediated by the cytoplasmic tail of KIR and regulated at the post-transcriptional level. PKC inhibition, metabolic labeling and colocalization studies demonstrated that the activation of the conventional PKCs upregulated surface and cellular KIR levels by stimulating the maturation processes in endoplasmic reticulum-Golgi and by promoting the recycling of surface KIR through sorting endosomes. Similar studies also revealed that KIR was secreted to plasma membrane through lytic granules in a PKCdelta-dependent manner. Consequently, PKCdelta inhibition caused the formation of giant perinuclear granules, which trapped KIR and FasL as well as CPE and Lamp1.Immunology and Cell Biology 01/2007; 85(3):220-8. DOI:10.1038/sj.icb.7100031 · 4.21 Impact Factor
[Show abstract] [Hide abstract]
ABSTRACT: Lewy bodies (LBs) are pathological hallmarks of Parkinson disease (PD) but also occur in Alzheimer disease (AD) and dementia of LBs. Alpha-synuclein, the major component of LBs, is observed in the brain of Down syndrome (DS) patients with AD. Dyrk1A, a dual specificity tyrosine-regulated kinase (Dyrk) family member, is the mammalian ortholog of the Drosophila minibrain (Mnb) gene, essential for normal postembryonic neurogenesis. The Dyrk1A gene resides in the human chromosome 21q22.2 region, which is associated with DS anomalies, including mental retardation. In this study, we examined whether Dyrk1A interacts with alpha-synuclein and subsequently affects intracellular alpha-synuclein inclusion formation in immortalized hippocampal neuronal (H19-7) cells. Dyrk1A selectively binds to alpha-synuclein in transformed and primary neuronal cells. Alpha-synuclein overexpression, followed by basic fibroblast growth factor-induced neuronal differentiation, resulted in cell death. We observed that accompanying cell death was increased alpha-synuclein phosphorylation and intracytoplasmic aggregation. In addition, the transfection of kinase-inactive Dyrk1A or Dyrk1A small interfering RNA blocked alpha-synuclein phosphorylation and aggregate formation. In vitro kinase assay of anti-Dyrk1A immunocomplexes demonstrated that Dyrk1A could phosphorylate alpha-synuclein at Ser-87. Furthermore, aggregates formed by phosphorylated alpha-synuclein have a distinct morphology and are more neurotoxic compared with aggregates composed of unmodified wild type alpha-synuclein. These findings suggest alpha-synuclein inclusion formation regulated by Dyrk1A, potentially affecting neuronal cell viability.Journal of Biological Chemistry 12/2006; 281(44):33250-7. DOI:10.1074/jbc.M606147200 · 4.60 Impact Factor
Article: Calpain-resistant fragment(s) of alpha-synuclein regulates the synuclein-cleaving activity of 20S proteasome[Show abstract] [Hide abstract]
ABSTRACT: Alpha-synuclein is a pathological component of Parkinson's disease by participating in Lewy body formation. Imbalance in protein turnover could result in the abnormal protein aggregation responsible for eventual neuronal cell death. This in vitro digestion study showed that both m-calpain and 20S proteasome preferentially hydrolyzed the N-terminal half of alpha-synuclein, which made the hydrophobic NAC and following acidic C-terminal region resistant against the proteolyses. Since the acidic C-terminal region contains the PEST segment-a protein degradation signal enriched with amino acids of proline (P), glutamate (E), serine (S), and threonine (T)-, the PEST segment has not been processed or even required for the proteolyses. Alpha-synuclein would be recognized primarily by m-calpain since the common substrate was processed by m-calpain five times more effectively than 20S proteasome with k(cat)/K(m) of 1.64 x 10(4)M(-1)s(-1) and 0.32 x 10(4) M(-1)s(-1), respectively. The N-terminally truncated protease-resistant C-terminal fragment of alpha-syn61-140 was demonstrated to stimulate the 20S proteasome-mediated breakdown of alpha-synuclein and its mutant forms of Ala53Thr and Ala30Pro. The stimulation for Ala53Thr, however, was noticeably less efficient than those for the other proteins, which might support the previous observation of the prolonged intracellular life span of Ala53Thr by 1.5-fold compared to that of wild-type form. We have hypothesized that the N-terminally truncated C-terminal fragment derived from the abundant alpha-synuclein through intracellular proteolyses could be involved in various physiological or pathological effects which might be related to the formation of abnormal protein aggregation and subsequent neuronal degeneration by influencing the intracellular protein turnover or directly participating in the aggregate formation.Archives of Biochemistry and Biophysics 12/2006; 455(1):40-7. DOI:10.1016/j.abb.2006.08.019 · 3.04 Impact Factor
Article: Amino acid sequence motifs and mechanistic features of the membrane translocation of alpha-synuclein.[Show abstract] [Hide abstract]
ABSTRACT: Many lines of evidence suggest that alpha-synuclein can be secreted from cells and can penetrate into them, although the detailed mechanism is not known. In this study, we investigated the amino acid sequence motifs required for the membrane translocation of alpha-synuclein, and the mechanistic features of the phenomenon. We first showed that not only alpha-synuclein but also beta- and gamma-synucleins penetrated into live cells, indicating that the conserved N-terminal region might be responsible for the membrane translocation. Using a series of deletion mutants, we demonstrated that the 11-amino acid imperfect repeats found in synuclein family members play a critical role in the membrane translocation of these proteins. We further demonstrated that fusion peptides containing the 11-amino acid imperfect repeats of alpha-synuclein can transverse the plasma membrane, and that the membrane translocation efficiency is optimal when the peptide contains two repeat motifs. alpha-Synuclein appeared to be imported rapidly and efficiently into cells, with detectable protein in the cytoplasm within 5 min after exogenous treatment. Interestingly, the import of alpha-synuclein at 4 degrees C was comparable with the import observed at 37 degrees C. Furthermore, membrane translocation of alpha-synuclein was not significantly affected by treatment with inhibitors of endocytosis. These results suggest that the internalization of alpha-synuclein is temperature-insensitive and occurs very rapidly via a mechanism distinct from normal endocytosis.Journal of Neurochemistry 05/2006; 97(1):265-79. DOI:10.1111/j.1471-4159.2006.03731.x · 4.24 Impact Factor
Top Journals
Yonsei University Hospital
Sŏul, Seoul, South Korea
- • Surgery
- • Department of Internal Medicine
Yonsei University
Sŏul, Seoul, South Korea
- • Department of Microbiology
- • Department of Forensic Medicine and Brain Korea 21 Project for Medical Science
- • Department of Biology
- • Institute for Immunology and Immunological Diseases
Inha University
Chemulpo, Incheon, South Korea
- Department of Biochemistry


text:
Beco Gemini
I love the beco gemini. I've tried the k'tan wrap and the baby bjorn but both were uncomfortable and baby didn't like it. With the beco, i can wear baby facing in or out. We can go for long walks both enjoying the activity without feeling pain or discomfort. I'm fairly petite 5'2 so smtg to keep in mind as my hubby has tried it and it doesn't seem to fit him as well.
I love, love, love my Beco Gemini! Love that I can use it from newborn until toddler ages and all the positions I can use.
sahm of two girls and a boy. 2009. 2010. 2014
I bough the Beco Soleil after I started to hate the bjorn, and my son was too awkward in the ktan (was great when he was first born) And I have loved it since day 1. It is the absolute best purchase of my mommyhood!


text:
Early offense leads Bucs over Dodgers
July 24, 2014 PITTSBURGH — Clint Hurdle isn't sure the Pittsburgh Pirates need an ace to make it to the postseason for the second straight year. more »»
Pirates get back to winning ways
July 23, 2014 PITTSBURGH — Clint Hurdle watched rookie Gregory Polanco scuffle for the better part of two weeks before giving the talented but raw right fielder a needed night off on Monday. more »»
Bucs' home win streak snapped
July 22, 2014 PITTSBURGH — A sharp Hyun-Jin Ryu and some clutch hitting enabled the Los Angeles Dodgers to overcome the absence of two top sluggers. more »»
How sweep it is
July 21, 2014 PITTSBURGH — Taking the extra base was the key for the Pittsbur'gh Pirates in completing a three-game sweep of the Colorado Rockies. more »»
Pirates' resiliency leads to come from behind victory
July 19, 2014 PITTSBURGH — Travis Snider's pinch-hit double in the eighth scored Neil Walker to spark the Pittsburgh Pirates to a 4-2 victory over the Colorado Rockies on Friday night. more »»
Pirates pass first half test, but there is work still ahead
July 18, 2014 During any given school year between first grade and senior year of high school, I stressed out at least four times every 10 weeks. more »»
Reds top Pirates
July 14, 2014 CINCINNATI — Kris Negron waited a long time for his first major league home run. When it came, he was in a hurry to celebrate with his teammates. more »»
Pirates blow 4-run lead in 6-5 loss to Reds
July 12, 2014 CINCINNATI — Brayan Pena's pinch-hit single in the eighth inning completed another late rally by the Reds, who overcame more injuries on Friday and beat the Pittsburgh Pirates 6-5 to keep their... more »»
Volquez deals Cards a 9-1 loss
July 11, 2014 ST. LOUIS (AP) — Pittsburgh Pirates right-hander Edinson Volquez was expecting some pearls of wisdom from pitching coach Ray Searage before Thursday night's start at St. Louis. more »»
Cards on verge of sweep as Lynn baffles Pirates
July 10, 2014 By R.B. FALLSTROM Associated Press ST. LOUIS — Lance Lynn reached double figures in wins before the All-Star break for the third straight year and the S. more »»
Adams walks off Bucs
July 8, 2014 ST. LOUIS — After beating the team he grew up rooting for with his first career game-winning hit, Matt Adams couldn't resist a celebratory fist pump just a few steps out of the batter's bo. more »»
Locked down
July 7, 2014 PITTSBURGH — Jeff Locke spent a lot of time with A.J. Burnett last year, and then faced his mentor in a pitching matchup in Pittsburgh. more »»
McCutchen gets starting nod
July 7, 2014 NEW YORK — The trade that put Jeff Samardzija on a postseason contender cost him a chance to pitch in his first All-Star gam. more »»
McCutchen, Watson, Harrison make 2014 All-Star roster
July 6, 2014 Rosters for the MLB All-Star game on Tuesday, July 15 at Target Field, Minneapolis (x-inactive; r-replacement). more »»
Barmes placed on 15-day disabled list
July 6, 2014 PITTSBURGH (AP) — The Pittsburgh Pirates have placed shortstop Clint Barmes on the 15-day disabled list Sunday with a left groin strain. Barmes' move to the DL is retroactive to June 30. more »»
Philadelphia Freedom
July 5, 2014 PITTSBURGH — When Andrew McCutchen stepped to the plate in the eighth inning, he wasn't looking for a home run but he thought he had one to complete a cycle. more »»
Polanco rattles Arizona
July 3, 2014 PITTSBURGH — The first slump of Gregory Polanco's major league career didn't last very long. more »»
July 2, 2014 PITTSBURGH — Starling Marte swung so miserably at the first two sliders he saw from Arizona Diamondbacks closer Addison Reed in the ninth inning that the Pittsburgh left fielder knew more were on th... more »»


text:
Is this product automatic defrost?
i just love this freezer comparing to my old freezer this freezer does not freezer up as much so in that case i will not have to defrost as much. I like that it is stainless steel and fits nicely in my small condo kitchen. I did not want the regular old white freeze so when a friend suggest that i check out Overstock i did and i found what i was looking thanks overstock....
SPT 3.0-cubic-foot Upright Freezer
This freestanding commercial upright freezer from SPT features a flush-back design, making it perfect for smaller kitchens. The door can be installed to open from the right or the left, and the four drawers make organizing a snap.
- Details
- Shipping & Returns
- Reviews
- Enhance your kitchen decor with a 3.0-cubic-foot upright freezer
- Freezer's compact, flush back design is perfect for with tight spaces
- Reversible doors offer versatility
- Features four pull-out drawers, adjustable thermostat, lock, and key
- 3.1 cubic-foot. net capacity
- Manual defrost
- Lock and key
- Reversible door
- 4 pull-out drawers
- Top 3 baskets: 14 inches wide x 12.5 inches deep x 5 inches high
- Bottom basket: 14 inches wide x 6.5 inches deep x 7 inches high
- Ice cube tray
- Mechanical adjustable thermostat
- Flush back design for space saving
- Front leveling legs
- Recessed handle
- Flush back design
- Freestanding application
- UL approved and Energy Star rated
- Input voltage: 115V / 60Hz Amperage 1.2A
- Temperature range: 5 to 11 degrees Fahrenheit
- Type direct cooling
- Net capacity: 3.0 cubic feet / 87 liters
- Energy consumption: 299k Wh/year
- Noise level: 40 dBA
- Refrigerant R134a Climate Class
- Measures 19.88 inches wide x 20.67 inches deep x 33.46 inches high
- Package dimension: 22.3 inches wide x 22.7 inches deep x 34.9 inches high
- Net weight: 66 pounds
- Gross weight: 70 pounds
|Cubic Feet
|Up to 5 cu ft
|Product Features
|Energy Star Compliant Variable Temperature Control
|Manufacturer Warranty
|1 year Limited Manufacturer
|Model Number
Customer Reviews
- Disclosures & Guidelines
- Filter By:
- Most Helpful
- Most Recent
Works but rattles.
I have to keep playing with the feet and door to make the noises stop.Read More
Love my new freezer
i just love this freezer comparing to my old freezer this freezer does not freezer up as much so in that case i will not have to defrost as much. I like that it is stainless steel and fits nicely in my small condo kitchen. I did not want the regular old white freeze so when a friend suggest that i check out Overstock i did and i found what i was looking thanks overstock.Read More
Fits my needs. I just wanted a little more space then what I had with my side by side.Read More
A Pleasant Surprise
I was so surprised to find this freezer after having looked for several years. I wanted a freezer with a stainless steel door, to match my other appliances. I also wanted the door facing front, as opposed to being on top. Well, here came SPT to the rescue. It provided everything I had been looking for and I couldn't be happier. Overstock delivered it quickly, and it was packed tight. I would recommend this to anyone looking for an extra freezer for the home.Read More
Very Happy with product!
Very happy with my purchase. This was exactly what I was looking for and I went by the reviews when purchasing it. Delivery was right on time and they left the unit outside by the garage. It was packed nicely and easy to use. Just plug in. If you are looking for a small freezer, this is it! Was very happy with Overstock customer service also. They were able to accommodate all of my needs.Read More
SPT 3.0 freezer
Have in my Laundry room and since it is stainless, it looks very nice. Plugged in and started right up. Keeps extras frozen just like large freezer. Really nice quality. Am hoping I won't have to defrost it too often though, as it is not self-defrosting.Read More
Needs defrosting
Holds a lot I either I did not read description right or it's not there but it's not self defrost. It defrosts quickly holds a lot other then the defrosting great product.Read More
So Far So Good
I've had this about a month now. Shipping was wonderful. love the extra storage it gives me. I miss looked at the picture and thought it was a top loading freezer (again my bad) but dosent bother me as it's actually easier then a top loading.Read More
Freezer functions well
This freezer functions well and provides us with the extra freezer space that desired. Unfortunately, the temperature control is on the rear of the unit and not inside so any tweaks are not simple.Read More
Exactly what I was looking for
I searched every major home improvement store and found this exact freezer at Home Depot.com. Just out of curiosity, I searched Overstock.com to see if they had it and they did. I have had such a great experience with everything I have purchased thus far from Overstock.com that I decided to order it from them. I received the freezer in two days and am absolutely thrilled with it. It's everything I wanted and more. I've received several compliments on how "pretty" it is and I can't believe how much food I can fit into it. I wish I would have purchased this years ago and so far, it's working perfectly! I couldn't be happier with my purchase!Read More
Questions & Answers
Hello juliabee, this product only features a manual defrost system. If you have any additional questions please feel free to let us know. Thank you for shopping with us!
How cold will this freezer get to?
Hello okayidontknow, the temperature range on this freezer is 5-11°F. Hope this information was helpful. Thank you for shopping with us!
How long is the cord?
Hello carrie313, The power cord is 5 ft and 6 inches. Thank you for shopping with us.
What is the model number of this freezer?
Thank you for contacting us diski54. The model number is:UF-311S. Please let us know if you have any further questions.
I live in a non doorman, non elevator building in nyc. Does the free shipping include delivery to my apt door (2 flights up)? If not, how much add'l cost should I expect?
Hello sheshbot, The free shipping does not include delivery to a 2nd floor apartment. When you order this item, you can request "White Glove" shipping. The best thing to do, is to call Overstock.com so that the proper shipping is set up that will be the most convenient for you and also the most cost saving. You can reach Overstock.com at: 800-843-2446. Thank you for shopping Overstock.com, let us know if you have any additional questions.
what color is this? it looks like a bronze color. or is it stainless steel?
Thank you for your interest in the SPT 3.0-cubic-foot Upright Freezer. After looking over the reviews that have been left by customers, it would be stainless steel.
If the item arrives and it does not work, who is responsible for the return shipping?
We will be responsible for the return shipping.
Shopping Guides
New Trends in Kitchen Appliances
Updating your kitchen can refresh your whole home; whether you're doing a kitchen-wide renovation or just updating some of the basics, kitchen appliances are a good place to start. Kitchen appliances include everything from toasters to freezers, coffee pots to blenders, hand mixers to Panini presses; no matter how big or small you want to start, check out the new trends in kitchen appliances and get going on your perfect kitchen.
Treadmills vs Elliptical Trainers
New variations of tried-and-true exercise machines are always being introduced to the market as the technology of exercise equipment improves. Two exercise machines that have gained popularity are elliptical trainers and treadmills. A major advantage to elliptical trainers is that they possess little risk of injury to the knees, hips, back or ankles. Meanwhile, treadmills are home gym machines on which you can run, walk or jog. The treadmill has been in the marketplace for a long time and is a long-standing piece of standard equipment for most exercise enthusiasts. Elliptical Machines: Elliptical machines present a lower risk of injury to the body. This home gym machine allows the individual to stand in an upright position while supporting her upper body by holding onto the handrails. The individual is able to pick out what muscles to train by shifting the strength on the legs or the arms. This provides for a more flexible workout machine. The machine bears its name because it works to copy the foot's elliptical motion as well as the rotation of the hip during walking or running. Advantages of Elliptical Machines: Since elliptical machines work the upper and lower body, they help you reach your target heart rate more optimally, providing a better cardiovascular workout overall. Individuals can burn a larger number of calories in a shorter period of time. Muscles that benefit from this machine include the legs, shoulders, chest, biceps, triceps and the back. Elliptical machines are also relatively easy to operate and are energy efficient because they do not use much electricity. Treadmills: Traditional treadmills were developed to help you walk, run or jog. Treadmills differ in advantages as there are some machines that have programs that can be customized based on your weight and fitness level. You can also manually control the program to increase or decrease the speed, the incline or the duration of the workout. Walking on a traditional treadmill, however, creates more impact on the knees and hips and may be a cause of injury for some users. Advantages of Treadmills: Some treadmill models have a cushioned surface to support the knees and other parts of the body. With these models, there is a lower risk of injury and an increased level of comfort for the user. Moreover, treadmills provide you with the option to run, jog or walk in the comfort of the gym. Also, you can burn calories by increasing the heart rate, providing a good cardiovascular workout. Buy Home Gym Machines Back to Guides Directory Buy Home Gym Machines
Refrigerator Fact Sheet
In the world of large appliances, there are many different sizes and types of refrigerators available. Because there are so many options, it is often hard to decide which would be best for your particular situation. A refrigerator is a large appliance that you will use for years and years to come, so consider the following when deciding the type and size of your refrigerator.
Types of Refrigerators
Of all the large appliances in your kitchen, your refrigerator is definitely one of the most important. If you're in the market for a new refrigerator, chances are you want to make sure you buy the right refrigerator for your home and family. There are several different kinds of refrigerators, and each one has its pros and cons. Learn more about the different types of refrigerators below.
Green Appliances Buying Guide
If you want to go green, having an environmentally friendly home is important; but with the choices out there, how do you really know if you are buying the most eco-friendly appliances and housewares? Sure, they may advertise themselves as green, but there are some things to keep in mind before simply believing them. This green appliances buying guide will help you know what to look for in your washers, refrigerators and other large appliances.
Popular LG Appliances
LG is known most widely for electronics, but consumers have been enjoying LG appliances for many years. From LG washers and dryers to LG microwaves, the company has spent years developing a line of large appliances that are energy efficient, durable, user-friendly and affordable, and the current line of LG appliances is better than ever.
Tips on Keeping Your Laptop Cool
If you use your laptop computer for long periods at a time, especially with it sitting on the couch, bed or your lap, its base can get very hot. Although most laptops have a built-in fan, it may not be enough to keep your computer and sensitive components cool. You may need to find ways to keep your laptop cool to prevent the development of heat-related problems, such as unexpected shutdowns. Using computer accessories, like PC stands, and following a few simple tips can help get air flowing underneath your laptop.
Special Features of LG Appliances
When you think of appliances that feature unique style and a reputation for quality, LG appliances are probably not far from your mind. LG home appliances always seem to blend great features with a sleek and modern look. With any appliances for your home, however, you're probably looking for performance as well as style. To help you in your search for quality machines for your home, here are some of the highlighted features that LG appliances have to offer.
How to Clean Garbage Disposals
Did you know that odors can build up in garbage disposals around the blades, under the rubber rims and in the drain and pipes? Most people clean their kitchen sinks regularly but forget about one of the most important parts of kitchen sink, the garbage disposal. It can be tricky to reach into the disposal to remove the offending smelly residue, but with a little effort, you can quickly restore freshness to your drains. Using simple methods, you can degrease and deodorize garbage disposals while sharpening the blades to reduce future odor accumulation.
Tips on Using Beer Glasses
Enjoying a cold beer is a delight, and the right beer glasses can make the experience even better. Different types of beer are traditionally paired with different meals and even changes in the weather, but more important than knowing the "right" beer is choosing the appropriate beer glasses. Different beers have different qualities, and enjoying them from the proper barware can enhance the beer drinking experience.
How to Shop for a Refrigerator
As the most frequently used appliance in your kitchen, your refrigerator is the centerpiece of the entire room. You'll likely keep your new refrigerator for ten years or more. So, the more you know about selecting the right fridge, the more you're likely to enjoy using it and save money on energy costs. Here are some steps to follow to make sure you cover the important points in choosing your new refrigerator.
FAQs about Food Storage
Whether you are setting up an emergency supply of food or simply stocking your pantry with kitchen storage items, it's important to know how long the different foods in your kitchen storage last as well as methods to increase their shelf life. Preparing foods as directed and storing them properly not only maintains the quality of the product; it also helps you avoid food poisoning. Read on for answers to your questions about food storage.
Home Appliances Buying Guide
The convenience and comfort that comes from large appliances are a significant part of nearly every modern home. This home appliance buying guide from Overstock.com is designed to help you know just what to look for when shopping for large appliances for your home.
Designing Your Home with GE Appliances
The quality of the appliances that you use in your home can have a significant impact on your quality of life. And having large appliances that last will save you money in the long run. That's why GE large appliances find their way into so many homes. GE appliances have the iconic GE style and offer the latest technology available in large appliances for the home. This guide will give you some ideas for using GE appliances in your home and the benefits that come with them.
How to Find Large Appliances to Fit Your Life
Large appliances help make maintaining a home much easier and more convenient. But whether you have just bought a house and are looking to outfit it with large appliances or you're upgrading what you already have, you may be unsure about which ones are right for your home. These tips will help you find the right appliances for you and your lifestyle.
Tips on Baking with Pecans
Everyone knows pecan pie is delicious, but did you also know pecans contain oleic acid; are rich in vitamin E, thiamin, magnesium, and copper; and are a good source of fiber? Here are some more helpful tips about pecans and some recipes that will make you want to dig out your favorite baking tools and celebrate the pecan every day of the year.
How to Cut Your Power Bill in the Kitchen
Electricity costs are constantly on the rise, but the kitchen is an excellent place to cut down on your power bill. With just a few simple steps, you can easily save money every month. Plus, you can also decrease your footprint on the environment. Check out these money-saving steps that will teach you how to cut your power bill in the kitchen.
Top 5 Advantages of Stainless Steel Appliances
Restaurants and commercial kitchens have been using stainless steel appliances and equipment for decades because stainless steel boasts a long life span, is easy to maintain and is among the easiest materials to keep clean. When you're outfitting your kitchen with new small and large appliances, you'll find that there are at least five advantages of choosing stainless steel for your refrigerator, oven range, dishwasher and other household appliances.
FAQs about Silicone Bakeware
Bakeware has come a long way over the past few years. Metal and glass have given up their spots as the state-of-the-art of baking in lieu of more temperature-stable silicone bakeware. Silicone bakeware is nonstick, flexible, and cooks very evenly, and it does it all using less oil, butter and shortening.
Tips on Teething Pain Relief
Seeing your baby in pain can be heartbreaking, and teething is painful for most babies. While some babies hardly shed a tear when those pearly whites show up, most do suffer some amount of discomfort. When babies begin teething, the gums become very sensitive. Not only is teething uncomfortable for your baby, but the pain caused by infant teething can interrupt sleep schedules and interfere with eating, making teething woes even worse. Luckily, there are some things you can do to bring your child relief from baby teething pain. Read on to learn about the best methods for relieving teething pain.
Shipping & Returns
Standard Return Policy:
Items must be returned in new or unused condition and contain all original materials included with the shipment. More Details
FINAL SALE EXCLUSION: Items marked as FINAL SALE are not returnable unless the problem you experience is the result of our error.
Shipping Information:
Our standard shipping method is ground shipping. We also offer expedited shipping for most items. Your final
shipping cost and estimated delivery timeframe will be available at checkout.
Standard shipping details
Expedited shipping details
* For your protection, all orders are screened for security purposes. If your order is selected for review, our Loss Prevention Team may contact you by phone or email. There may be a two business day delay to process your order.
** Most Oversize orders are delivered within 1-4 weeks. Some orders may take 6 weeks to be delivered.


text:
Clocking In
You have clocked in to MySA.com's business news blog about San Antonio and South Texas businesses and business people.
Cullen/Frost declares dividend
Cullen/Frost Bankers Inc. declared a second-quarter cash dividend of 51 cents a share, a penny increase from the company's previous dividend for holders of its common shares. The dividend is payable June 13 to shareholders of record on May 30. Cullen/Frost is the parent of Frost Bank, the largest regional bank based in San Antonio. The company's board […] [Read More]
Revenue on the rise for local entrepreneurs
About 70 percent of San Antonio-area entrepreneurs who responded to a recent survey said they booked higher than average revenue in the last six months. The survey included 94 San Antonio-area companies with at least $1 million in annual revenue. "The results from the survey underscore that San Antonio businesses are thriving despite the current […] [Read More]
Faith Family Clinic awarded $35,000 for chronic disease management
Faith Family Clinic, a San Antonio medical home for the working uninsured, has been awarded a $35,000 grant by the CVS Caremark Charitable Trust to fund a chronic disease management program. The grant will allow the clinic to more closely monitor patients with chronic conditions, such as diabetes, hypertension and high cholesterol. The program aims […] [Read More]
SPBS acquires locally based Southern Medical Services
The clinical equipment service company SPBS has acquired a San Antonio medical equipment repair company. The locally based Southern Medical Services is now part of SPBS' portfolio. Before the acquisition, the companies worked side by side for more than 30 years. As a result of the merger, SPBS will provide stronger repair and maintenance services, […] [Read More]
Diversity council recognizes CST Brands chief
The National Diversity Council ranked Kim Bowers, chairman and CEO of locally based CST Brands Inc., among its list of female executives, influencers and achievers impacting the oil and gas industry. Earlier this week, the organization released its Top 50 Most Powerful Women in Oil and Gas of the year and recognized Bowers in her role […] [Read More]
Job fair for teachers and administrators
The Archdiocese of San Antonio's Department of Catholic Schools is hosting a job fair for teachers and administrators Saturday morning. The fair is from 9 a.m. to noon at the St. Luke Catholic School Parish Hall, 4603 Manitou Drive. Interested applicants are asked to bring resumes and copies of a completed application as interviews will […] [Read More]
James Avery expands to Frisco
James Avery found a new home this week in Frisco. The family-owned, Kerrville-based jewelry retailer opened a store there on Tuesday at 2601 Preston Road in the Stonebriar Centre, it announced in a news release. Less than a mile away, James Avery operates another store on Preston Road, though within Plano city limits, according to its […] [Read More]
Walmart looking to hire nearly 100 workers
Walmart on Tuesday announced it will hire up to 95 employees to staff its new Neighborhood Market opening this summer on the West Side. The company plans to fill both part- and full-time positions, with the majority set to start in June to prepare for the store's grand opening at Military Drive West and Hunt […] [Read More]


text:
Was £12.00
Now £6.00
Now £3.60
70% Off
Save a total of £8.40
Out of stock
Ocean Pals
Item No.1710101634
Go on an undersea adventure with Moon Dough Ocean Pals. Little land lubbers can get a taste of life under the sea using Moon Dough's clever ' push and pop ' moulds. The easy-to-use tools help even little fingers create underwater scenes packed with octopuses, whales and turtles. Colourful, squidgy and cleverly designed never to dry out - Moon Dough provides hours of fun.
This item is available for
UK Home Delivery Free (UK orders over £30) or £3.99
Delivered within 4 working days
Next or Named Day Delivery
7 days a week between 8am - 6pm
Monday to Friday - order by 7pm - £4.99
Saturday & Sunday - order by 6pm - £4.99
Next or Named Evening Delivery
7 days a week between 6pm - 10pm
Monday to Sunday - order by 10pm - £6.99
Collect from store the next day - FREE
Order before 7pm for next day collection after 12pm midday
Show participating stores
Full Delivery details
- Ages 3+
- Includes: 3 Push 'n' Pop tools 1 mould 2 colours of Moon Dough
- Moon Dough never dries out
- Please note: Moon Dough is not edible


text:
Tony-nominated lyricist and playwright to speak at Morningside College
Tony-nominated lyricist and playwright Bill Russell will return to Morningside College on Tuesday, Oct. 21, to talk about the revival of his musical "Side Show" on Broadway in New York City.
Faculty member and students presents research in California
Jessica Pleuss, assistant professor of psychology at Morningside College, and two students recently presented research at the Association for Psychological Science's annual convention in San Francisco.
Morningside College will host district FFA soil judging contest on Sept. 30
High school students from approximately 40 school districts will participate in the Northwest Iowa District Future Farmers of America (FFA) soil judging contest on Tuesday, Sept. 30, at Morningside College.
Morningside College students go "Into the Streets" for a day of community service
More than 800 Morningside College students, faculty, and staff will leave the classroom to go "Into the Streets" for a morning of community service on Wednesday, Oct. 1.
Morningside College announces new faculty members in the arts and the humanities
Morningside College recently announced three new faculty members in the arts and the humanities.


text:
John Clayton (sportswriter)
|John Travis Clayton
May 11, 1954
Braddock, Pennsylvania
|Churchill Area High School
Duquesne University
|National Football League analyst
Sports radio host
John Travis Clayton (born May 11, 1954) is a National Football League (NFL) writer and reporter for ESPN. He is also a senior writer for ESPN.com.
Early career[edit]
Clayton began covering sports while still a student at Churchill Area High School. Starting with the Pittsburgh Steelers' 1972 training camp, he covered the team in twice-weekly dispatches in the St. Marys, Pennsylvania Daily Press. He later wrote for Steel City Sports, a weekly publication in Pittsburgh. In 1975, Steel City Sports changed into Score! Pittsburgh and Clayton was a staff writer, covering the Steelers. He also served as a stringer for a number of radio networks, including AP Radio, and covered games, providing the network with sound clips from locker room interviews after games involving Pittsburgh's professional sports teams.
Clayton graduated from Duquesne University in 1976, and later worked for The Pittsburgh Press. He had done part-time work for the paper while attending college.
In May 1978, Clayton was sent to cover a Steelers mini-camp in place of the Press' regular Steelers beat writer, Glenn Sheeley. While there he discovered and reported a rules violation which would cost the team a draft pick. The affair was dubbed "Shouldergate" by Clayton.[1] Clayton became persona non grata for some time in his hometown for his role in the affair.[2]
Clayton eventually worked his way up to become the Steelers beat writer at the Press, before leaving the paper in 1986.[3] He moved across the country and began covering the Seattle Seahawks for The News Tribune in Tacoma, Washington.[3] It was at this time that he began appearing in NFL segments on Seattle sports radio station KJR (AM) on host Nanci Donnellan's program "The Fabulous Sports Babe". When Donnellan's show was picked up by ESPN for national syndication, Clayton came along as an NFL correspondent.
In 1995, Clayton joined ESPN as a reporter and later added to his duties a weekly radio show during the NFL offseason. He hosted the show with former NFL quarterback Sean Salisbury; the show included "Four Downs," a debate with Salisbury over current NFL issues. Their debates often became quite heated, with Salisbury referring to Clayton as the "Cryptkeeper" and "Mr. Peabody", mocking his geeky and "eggheaded" appearance and voice, and Clayton responding by calling Salisbury "Mr. Backup" based on his limited playing time during his NFL career. There is debate as to seriousness of the animosity between Salisbury and Clayton.
Radio programs[edit]
Clayton remained a frequent contributor to KJR (AM), and hosted its "Sports Saturday" show on Saturday mornings. He is a regular caller to sports-talk radio stations around the country. Because of the transition to all-sports of KIRO (AM) Seattle, Clayton moved his show to the new ESPN station.
Awards and honors[edit]
Clayton received the Dick McCann Memorial Award from the Pro Football Hall of Fame in 2007.[4] This distinction puts him in the "writer's wing" of the Hall of Fame.[5]
He was also inducted into the sports hall of fame of his alma mater, Duquesne University, in 2001.[6]
On February 8, 2015 Clayton wrote an ESPN.com article claiming "Bill Belichick wasn't suspended for spying on practices."[7] Clayton's claim of New England Patriots coach Bill Belichick spying on practices is a widely discredited claim[8] by John Tomasse of the Boston Herald. The Boston Herald later issued a public apology. Clayton had previously written two articles[9][10] acknowledging that the rumor of taping practices was false before publishing the article on 8 February 2015 claiming it was true.
Clayton later added a correction but did not acknowledge that his original text incorrectly stated Bill Belichick was guilty of the infraction of taping practices. Clayton's correction instead claimed he reported the details of the punishment incorrectly, when he had actually reported the infraction incorrectly. Clayton's edit added, "A Feb 8 story on ESPN.com incorrectly reported details around New England Patriots coach Bill Belichick's NFL punishment in 2007." The original article did not make any statement about the details for New England Patriots coach Bill Belichick's punishment, and a correction was not necessary. Despite claiming to correct just the punishment details in the article, Clayton did correct the text to remove the false claim that Belichick taped practices.[7]
- ^ Clayton, John (June 1, 1978). "Steelers' Secret Slips Out". Pittsburgh Press. pp. C–10. Retrieved April 2, 2010.
- ^ McHugh, Roy (June 5, 1978). "To Report Or Not – That Is The Question". Pittsburgh Press. pp. C–1. Retrieved April 2, 2010.
- ^ a b "ESPN Reporter/Pittsburgh Native John Clayton". Duquesne University. March 27, 2007. Retrieved April 2, 2010.
- ^ "Clayton is 2007 McCann Award winner". Pro Football Hall of Fame. July 10, 2007. Retrieved March 30, 2010.
- ^ "John Clayton bio". ESPN. Retrieved May 4, 2011.
- ^ "Sports Hall Of Fame: Year of Induction List". Duquesne University. Retrieved March 30, 2010.
- ^ a b Clayton, John. "2015 Offseason off to rocky start". Espn.com. ESPN. Retrieved 8 February 2015.
- ^ Allen, Bruce. "Boston Herald apologizes to Patriots- Is it enough". bostonsportsmedia.com. Boston Sports Media Watch. Retrieved 14 May 2008.
- ^ Clayton, John. "After the big meeting, 5 things we learned about spygate". ESPN.com. ESPN. Retrieved 13 May 2008.
- ^ Clayton, John. "Minus a whistle-blower, Spygate will expire quietly". ESPN.com. ESPN. Retrieved 8 May 2008.
External links[edit]


text:
well i have only a few herbs...
Submitted by Key Largo 1948 on Mon, 06/18/2012 - 13:28. Permalink
.. on my balcony, but they smelled divinely this morning when i watered them. Also cosmoses from seeds to remind me of my paternal grandmother - mint and chives are for the maternal one.
Ron Paul ... forever.
- Login to post comments


text:
All What's New
- Today
- Past 7 Days
- Past Month
- Past Year
- Specific Date Range
- Feature Story (4)
- Press Release (1)
- Date
Latin America: A thirsty region with abundant water sources
On World Water Day we invite you to discover the most important challenges that a region like Latin America faces, through cartoons drawn for the World Bank Group by artists from around the world.&nbs... Show More +
Why do women earn less than men?
Potential actionsAlthough the winds are favorable with respect to women's labor market participation in Latin America, more actions should be taken to reduce the wage gap and favor women's school... Show More +
Multifaceted climate change has to be attacked on several fronts
The die is cast. If we do not act now, rising temperatures will endanger crops, freshwater reserves, energy security and even our health. We talked to World Bank environmental specialist Daniel Mira-S... Show More +
Brazil, Colombia and Peru are among the countries of the world with the most water
Protecting a non-renewable resourceJust 2.5% of the world's water can be used. This water is found in rivers, lakes and snow peaks, among other locations. As demand for water increases, cities are for... Show More +
Breaking the Cycle of Chronic Poverty in Latin America and the Caribbean
New analysis highlights need for better coordination and a focus on behavioral barriers alongside effective social and economic policies.LIMA, Peru, March 9, 2015 – One out of every five Latin America... Show More +


text:
Charlie Banana® Diaper Pail
by Charlie Banana
(Item #1343404)
Baby Pink
Perfect to finish your Charlie Banana Collection. This hanging diaper pail is made of a waterproof outer layer, which is ideal for storing your dirty cloth diapers or clothes.
More Colors white turquoise black royal blue hot pink
"I needed a dress for my brother-in-laws wedding and I had to be able to nurse in it easily. My daughter is only 4 months old so I knew she would be on and off for the entire day. The selection of dresses was outstanding and I was able to get free shipping at the time. This encouraged me to order three dresses and pick one that I liked the best. Two of the three dresses looked really nice on me"
Amberly in Peabody, MA
Copyright © 2015 Figure 8 Maternity. All Rights Reserved.


text:
today's highlight.
the manager telling the guys they need to listen to to what i tell them when i say how i want something done! now i have no authority to make anyone do anything and i am not in any position of authority in the shop. but the manager knows i make sure things are done correctly and with the companies and the customers best interests in mind at all times.
i told the other mechanic to remove the starter off a small trenching machine and carry it down to my brothers shop, (we do alot of business with my brothers shop for starters, alternators, batteries and electrical components) and get the starter drive replaced. he took it down there and replaced it with a brand new $250 starter! which could have been repaired for about $50-60 instead. the starter worked fine except for the starter drive. he tried to say it was faster to just buy the new one than wait for it to be repaired. if he had taken if oof when i suggested instead of dragging around and got it down to my brothers shop, it would have been repaired by this afternoon. besides, we had another trencher if we needed to rent one and we could still have gotten the unit back in service by late tomorrow morning. the manager wasn't happy in the least as i wasn't either. this was a clear waste of money IMO. the manager was mainly upset because he took it upon himself to make the decision instead of doing it the way i suggested, andif he has any question about what i suggest that he can take up with him and let him make a determination on the best course of action.
now even though i am not in a position of authority, i am given a lot of leeway and a lot of trust to get things done, and they know i make sure things are taken care of and done properly. i many times work unsupervised when working outside the shop and i am allowed to use my best judgement to get things done. i don't tell people at work what to do, or issue orders, i simply point out things that need to be done and how they should be taken care of. whether they get completed is not my affair and i allow people to make their own choices. i know what i need to get done and how it needs to be done and that's what i worry about.
My work partner sometimes talks about his adult son that still lives with him and his wife. The kid is in his mid to late twenties.
Today we found ourselves near his house at lunch time so, he invited me to have lunch at his house.
He took me inside and showed me around the kitchen before disappearing into the bathroom.
So, now I'm standing in the kitchen alone and it occurred to me that if the son were to discover a stranger in the home, it could be tragic. I started imagining what I could do if that happened. Then, a giant man (looked to be about seven feet tall) turned a corner and faced me. I started splainin' real quick.
"Christopher? I work with your father. We came home for lunch."
He wasn't concerned at all. He just said "okay" and went back to his room.
I don't scare easily, but that scared the crap outta me.
My partner later told me the "boy" is 67. Semantics
No offense and none taken (̿▀̿ ̿Ĺ̯̿̿▀̿ ̿)̄
Seems like a large 'man' in his 20's shouldn't be locked up in his room all day. Isn't there a school or job that would like his presence?
Sent from my iPad using Firearms Talk
He could be the next "Herman Munster" if the program ever came back.
Highlight of the day, finding the bottle of NyQuil, fighting off one heck of a head cold.
I guess the "Boy" doesn't play sports. That much "tall" can't be coached. If he had any athletic ability he'd have been playing Left Tackle in the NFL...
Sent from my iPad using Firearms Talk
highlight of my day at work!
got lots of time driving and doing pick-ups and deliveries. was suppose to spend the day at the shop ordering parts and billing out work orders. but i'll do that tomorrow. plus i have several peices of equipment that i got parts in the other day that i need to tackle. tomorrow is going to be busy, i can already tell.
Highlight of the day was the neighbor across the street gave VikingGal and me her member pass to Filoli. http://filoli.org/ where we spent the day touring the extensive gardens and the massive Georgian mansion. It is simply a staggeringly beautiful estate. Love the gardens.


text:
Just inOn sale
- Under $50
- $50-100
- $100-250
- $250-500
- $500-1,000
- Over $1,000
- Jewelry
- Rings
Alexis Bittar
- Alexis Bittar
- Alexis Bittar Orbiting Leather Ring$195 couture.zappos.comDraw people into your galaxy of style with the Alexis Bittar™ Orbiting Leather Ring. Woven black leather. Polished metal bands. Sized ring. Imported. Face Height : 1 ⁄ 4 in. Face Width : 1 in. Face Length : 3 ⁄ 5 in. Weight: 0.2 oz.
- Pre-owned Alexis Bittar RingsGoldtone and silvertone metal baroque ring set with a large, green stone. In a pouch. Brand : ALEXIS BITTAR.
- Alexis Bittar Fine Silver Juniper Marquis Pavé Ring$2,415 liberty.co.ukWith clusters of sparkling brown diamonds and green tourmaline, this Alexis Bittar ring shimmers in any light. Wear during the day to create a focal point or in the evening to add sheer glamour. A timeless statement that will be a permanent favourite in your collection. FEATURES: Rich pavé of adriatic quartz. COMPOSITION & SIZE: Oxidized sterling silver ,Adriatic quartz, Brown diamond, Tsavorite, Green tourmaline. 4.5cm x 1.5cm.


text: Tankless water heaters are quickly becoming the most popular water heaters in the United States, and it's not hard to see why. These compact units can be installed almost anywhere in the home and don't need a pilot light constantly burning. Unlike tanked water heaters, which keep a large tank of water hot all day long, tankless heaters only heat water when an appliance or shower calls for hot water. This makes tankless heaters efficient and eco-friendly.


text:
P-Mation #41: A-Door-Able
Blue boy meets his lovely neighbor!4.46 / 5.00 10,082 Views
Otherworld - Quiet Luke
in da othawurld4.40 / 5.00 7,223 Views
Joe vs the Environment
In collaboration with Earth Hour 2015 and Pocoyo4.07 / 5.00 2,757 Views
Nice story and reminds me of the Terra+Edgar+Banon/Sabin/Locke scenario system. And..
@Z-10 First of all, it was Locke, and second of all, he was lying.
Defies the story.
In 2 Cyan said that Celes was naked in the next room.
Uhm what the crap? Sabin killed by a cat? must've been a kitten spawned from the deepest darkest parts of Hell itself to have killed Sabin >_>. and UGH, Celese and Oomaro?!?!?! UGH. Though I did like the Mortal Kombat fight.
when the girl fell down and farted
i fell from my chair when i heard the noise it was so funny what the gaurds said i cant stop laughing
My Favorite was Edgar's veiwpoint.
I loved how you spoofed/made a tribute to FFX. The way Gau and Umero's speech got worse when other characters showed up. The Mortal Kombat inclusion.
All of it was great.
...what exactly did happen to that cat? *eye twitch*