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Transferrin recycling and dextran transport to lysosomes is differentially affected by bafilomycin, nocodazole, and low temperature
- Günther Baravalle,
- Daniela Schober,
- Marlis Huber,
- Nora Bayer,
- Robert F. Murphy,
- Renate Fuchs
- … show all 6 hide
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The effects of bafilomycin, nocodazole, and reduced temperature on recycling and the lysosomal pathway have been investigated in various cultured cell lines and have been shown to vary dependent on the cell type examined. However, the way in which these treatments affect recycling and transport to lysosomes within the same cell line has not been analyzed. In the current study, we used fluorophore-labeled transferrin and dextran as typical markers for the recycling and the lysosomal pathways, respectively, to explore the morphology and the intravesicular pH of endocytic compartments in HeLa cells. The V-ATPase inhibitor bafilomycin selectively inhibited the transport of marker destined for lysosomal degradation in early endosomes, whereas the transport of transferrin to the perinuclear recycling compartment (PNRC) still occurred. The kinetics of transferrin acidification was found to be biphasic, indicative of fast and slow recycling pathways via early endosomes (pH 6.0) and PNRC (pH 5.6), respectively. Furthermore, the disruption of microtubules by nocodazole blocked the transport of transferrin to the PNRC in early endosomes and of lysosome-directed marker into endosomal carrier vesicles. In contrast, incubation at 20°C affected the lysosomal pathway by causing retention of internalized dextran in late endosomes and a delay in transferrin recycling. Taken together, these data clearly demonstrate, for the first time, that the transferrin recycling pathway and transport of endocytosed material to lysosomes are differentially affected by bafilomycin, nocodazole, and low temperature in HeLa cells. Consequently, these treatments can be applied to investigate whether internalized macromolecules such as viruses follow a recycling or degradative pathway.
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- Title
- Transferrin recycling and dextran transport to lysosomes is differentially affected by bafilomycin, nocodazole, and low temperature
- Journal
Cell and Tissue Research
Volume 320, Issue 1 , pp 99-113
- Cover Date
- 2005-04-01
- 10.1007/s00441-004-1060-x
- Print ISSN
- Online ISSN
- 1432-0878
- Publisher
- Springer-Verlag
- Additional Links
- Keywords
- Endocytic pathways
- Bafilomycin
- Nocodazole
- Low temperature
- Endosomal pH
- HeLa Ohio cells (Wisconsin strain)
- Industry Sectors
- Authors
- Günther Baravalle (1)
- Daniela Schober (1)
- Marlis Huber (1)
- Nora Bayer (1) (2)
- Robert F. Murphy (3)
- Renate Fuchs (1)
- Author Affiliations
- 1. Department of Pathophysiology, Center for Physiology and Pathophysiology, Medical University of Vienna, Währinger Gürtel 18–20, 1090, Vienna, Austria
- 2. PerkinElmer Life and Analytical Sciences Vienna, Vienna, Austria
- 3. Departments of Biological Sciences and Biomedical Engineering, Carnegie Mellon University, 4400 Fifth Avenue, Pittsburgh, Pa., USA


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tag:blogger.com,1999:blog-6246681883258645640.post1608590818068116437..comments2015-03-30T16:48:21.246-07:00Comments on Enjoying the Small Things: *happies*Kellehttp://email@example.com:blogger.com,1999:blog-6246681883258645640.post-83000166549425394502013-02-14T11:39:09.789-08:002013-02-14T11:39:09.789-08:00The game ZINGzo is awesome for sight words!The game ZINGzo is awesome for sight words!Jasminehttp://email@example.com:blogger.com,1999:blog-6246681883258645640.post-1629561820981103612013-02-11T20:39:22.850-08:002013-02-11T20:39:22.850-08:00Short & Sweet, hope you feel better and have f...Short & Sweet, hope you feel better and have fun at the par-tay tomorrow.

I'm off to watch my guilty pleasure of MobWives... you know, where every other word if the F bomb :)

Olivia {i am still learning}http://firstname.lastname@example.com:blogger.com,1999:blog-6246681883258645640.post-39359816589220238212013-02-11T06:11:39.700-08:002013-02-11T06:11:39.700-08:00sight word bingo rocks! Make some bingo cards to g...sight word bingo rocks! Make some bingo cards to go with those flashcards, kicking up the fun!Kerryhttp://firstname.lastname@example.com:blogger.com,1999:blog-6246681883258645640.post-74927271145678013832013-02-11T05:16:59.298-08:002013-02-11T05:16:59.298-08:00I completely understand the not feeling well. I am...I completely understand the not feeling well. I am thirteen weeks and still have the horrible first trimester symptoms!

I am loving Nella's little pig tales and Lainey's eagerness to learn. I have a three year old little boy who is reading and writing and soaks it all up like a sponge.

Hope you're feeling better! xx Lianehttp://firstname.lastname@example.com:blogger.com,1999:blog-6246681883258645640.post-19361756380504333092013-02-11T04:01:41.154-08:002013-02-11T04:01:41.154-08:00Love this Love this kimberlyfranzenhttp://email@example.com:blogger.com,1999:blog-6246681883258645640.post-34528192035350656622013-02-11T03:53:19.015-08:002013-02-11T03:53:19.015-08:00Lovely post. We too are practicing sight words. Wa...Lovely post. We too are practicing sight words. Washi tapped homemade cards are all over our dining room wall*~*Simply.Kim*~*http://firstname.lastname@example.com:blogger.com,1999:blog-6246681883258645640.post-40454599972550875752013-02-10T18:37:31.341-08:002013-02-10T18:37:31.341-08:00Beautiful post! And what a lovely little fairy gar...Beautiful post! And what a lovely little fairy garden :)

Sheree
thehartungs.blogspot.ca SHhttp://firstname.lastname@example.com:blogger.com,1999:blog-6246681883258645640.post-5389217896859882742013-02-10T09:44:10.801-08:002013-02-10T09:44:10.801-08:00Hi Kelle Am sitting here in beautiful manzanillo m...Hi Kelle
Am sitting here in beautiful manzanillo mexico and thinking about you and the upcoming birth of your baby. Im also thinking about you because my husband and I recently purchased a new slr canon rebel recommended by my photojournalist son and LOL we are both struggling to learn. But when I see your amazing photos that so beautifully capture "the moments" of your life I feel encouraged to lol keep reading the manual. Hoping to get the hang of it while here in mexico for the month. Such color, character and beauty here. And will be thinking of you and your family as you bring another baby into your beautiful blended family. Best wishes, kkstylehttp://email@example.com:blogger.com,1999:blog-6246681883258645640.post-28948015302093124782013-02-10T08:25:15.598-08:002013-02-10T08:25:15.598-08:00I love reading your posts....I love your happiness...I love reading your posts....I love your happiness and positivity. I think that most people realize that you aren't trying to portray a perfect life but who needs to read more negativity from this world? (forgive me, I'm still thinking of the post from a few weeks ago about instagram). You inspire me to live life with gusto and truly enjoy life. I am reading your book right now so I am in a "gushing" mood right now. AND, I'm so excited to read the news of your new little one arriving!!! Thanks for being a ray of sunshine and goodness.MGhttp://email@example.com:blogger.com,1999:blog-6246681883258645640.post-26244882431971943472013-02-10T05:48:49.583-08:002013-02-10T05:48:49.583-08:00So . . . who was she talking to?! I'm guessing...So . . . who was she talking to?! I'm guessing whoever it was, that was the best conversation of their day. :)Leighhttp://firstname.lastname@example.com:blogger.com,1999:blog-6246681883258645640.post-29192942789316447502013-02-09T19:19:18.333-08:002013-02-09T19:19:18.333-08:00Just in case you didn't already amaze me in so...Just in case you didn't already amaze me in so many ways...how in the world can you sit Indian style on concrete at 8+ months pregnant?!? You truly are superhuman!Kristahttp://email@example.com:blogger.com,1999:blog-6246681883258645640.post-50319889027065498182013-02-09T18:40:33.680-08:002013-02-09T18:40:33.680-08:00Adore your kids! If I got a random phone call from...Adore your kids! If I got a random phone call from a cute 3 year old, it'd make my entire weekend. Jennifer Goodhttp://email@example.com:blogger.com,1999:blog-6246681883258645640.post-7312781553685861822013-02-09T10:38:14.740-08:002013-02-09T10:38:14.740-08:00they are sponges at that age. i remember going ov...they are sponges at that age. i remember going over memory bible verses with my big chick. she could remember entire chapters! holy cow! hope you are feeling better today. *oh and i wrote about my valentine's party dream i had of you the other day on my blog. too weird!Farmgirl Paintshttp://email@example.com:blogger.com,1999:blog-6246681883258645640.post-89078717100095404562013-02-09T08:45:44.867-08:002013-02-09T08:45:44.867-08:00Kelle.... I am sorry that you are not feeling well...Kelle....
I am sorry that you are not feeling well!! I'm getting over a cold, myself. I enjoyed one week--one freakin' week--free of feeling sick, and now.... Something else!! Yuck. :-(
Nella has a little Valentine's Day birthday party planned? Fun!! For her, or Baby Boy? Do tell!! Do tell!! ;)
I love, love, love Lainey's classroom Valentine box!! It is a birdhouse!! Very creative!! ;-D
That third picture of Nella!! I cannot get over her facial expression!! It is like: "Who, me? A blueberry thief? I didn't do it!!"!! Too cute!! ;)
"Nella just ran off with my phone, and I heard her push a bunch of numbers and someone said hello. She's babbling from the hallway, so this is my cue before international cell phone charges show up on our bill.". Crack. Me. Up!! ;-D
--Raelyn

Raelynhttp://email@example.com:blogger.com,1999:blog-6246681883258645640.post-15054956362964407702013-02-09T08:01:56.718-08:002013-02-09T08:01:56.718-08:00End -of-preggo body aches. A very faint memory. Ho...End -of-preggo body aches. A very faint memory. Hope you feel better today. Sweet post. Thanks you for sharing!
Cheers,
CarylCaryl Rosehttp://email@example.com:blogger.com,1999:blog-6246681883258645640.post-27307272454998792662013-02-09T07:55:17.976-08:002013-02-09T07:55:17.976-08:00Snuggle up on that couch and get yourself a comfor...Snuggle up on that couch and get yourself a comforting cozy cup of something soothing. And rest up, soon to be Mamma of 3 :)
Meanwhile, your everyday happies make the rest of us happy too! Love your everyday photos.
C xbestof2sisters.comhttp://firstname.lastname@example.com:blogger.com,1999:blog-6246681883258645640.post-91024693153269375052013-02-09T07:37:43.003-08:002013-02-09T07:37:43.003-08:00Happies to you Kelle!! Have a great weekend with ...Happies to you Kelle!! Have a great weekend with your girls...I have a feeling baby boy just might join the party!! All the best to you!
MoniqueMoniquehttp://firstname.lastname@example.com:blogger.com,1999:blog-6246681883258645640.post-8616711775810165042013-02-09T07:21:53.499-08:002013-02-09T07:21:53.499-08:00My happies for the weekend is seeing this NEMO sno...My happies for the weekend is seeing this NEMO snowstorm come to an end! I can only hope that the rest of New England can clean out and get back to have a Happy weekend. I am sure there are many Happies about this snow that we got if you are a snowmobiler or skier, Thanks for making me think Happy thoughts and not UGGGS... Love to see those sweet girls pics.Tammiehttp://email@example.com:blogger.com,1999:blog-6246681883258645640.post-16101578437653457422013-02-09T06:39:44.356-08:002013-02-09T06:39:44.356-08:00Love that first pic. I'm getting so excited fo...Love that first pic. I'm getting so excited for your baby Kelle! Feels like a good friend is about to have a baby :) xoDanielehttp://firstname.lastname@example.com:blogger.com,1999:blog-6246681883258645640.post-90614580703491850162013-02-09T03:29:00.300-08:002013-02-09T03:29:00.300-08:00Feel better! I live in Northern Indiana (MI borde...Feel better! I live in Northern Indiana (MI border) and the drearies have taken over! We are supposed to have sun today so my fingers are crossed! I CAN'T WAIT TO SEE PICS OF THE VALENTINE BIRTHDAY PARTY!! Thanks for sharing your inspiring words, wisdom, and kindness with the world!Sarahttp://email@example.com:blogger.com,1999:blog-6246681883258645640.post-74607796966047846382013-02-09T01:24:56.058-08:002013-02-09T01:24:56.058-08:00<3 <3 <3<3 <3 <3Scrap Piehttp://firstname.lastname@example.com:blogger.com,1999:blog-6246681883258645640.post-28162463830893720692013-02-08T19:04:59.241-08:002013-02-08T19:04:59.241-08:00And ALL adorable little happies! Feel better soon ...And ALL adorable little happies! Feel better soon - lot of love your way!christina vegahttp://firstname.lastname@example.com:blogger.com,1999:blog-6246681883258645640.post-8082086195724312182013-02-08T18:48:44.931-08:002013-02-08T18:48:44.931-08:00OH I pray you feel better soon!! Have a Blessed we...OH I pray you feel better soon!! Have a Blessed weekend!!!Elmahttp://email@example.com:blogger.com,1999:blog-6246681883258645640.post-17710487396509909252013-02-08T18:41:55.448-08:002013-02-08T18:41:55.448-08:00That is very good advice. I hope you feel better s...That is very good advice. I hope you feel better soon. I feel sort of bad today too. It isn't really sickness. Just sadness I think. I can't get it to go away. Darn it.

I hope your weekend is filled with unlimited happy moments and 0 international calls. Life with Kaishonhttp://firstname.lastname@example.com:blogger.com,1999:blog-6246681883258645640.post-39555675098436741542013-02-08T18:33:25.767-08:002013-02-08T18:33:25.767-08:00So funny to see the term "happies" on yo...So funny to see the term "happies" on your blog! I often use that term on my own, I love listing my random happies. I always think of it as a silly word, but I love it...it makes me smile. Angelahttp://email@example.com


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I'm an attractive lady looking for someone to appreciate my assets and tolerate my shortcomings. Would love to meet someone to go dancing with or maybe take dance lessons if need be. I'm not much for sitting on the beach and soaking up the sun, however, I love walking along the water's edge later in the day, before the sun goes down.
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First Trailer for HBO's Aaron Sorkin Drama 'The Newsroom' Released (Video)
Jeff Daniels stars as an outspoken anchor in the drama that explores the inner-workings of a 24-hour cable news network.
First, he showed you behind the scenes of The West Wing, then he won an Oscar for his account of the creation of Facebook. Now, Aaron Sorkin is revealing the gritty side of The Newsroom.
STORY: Jane Fonda Joins Aaron Sorkin's HBO Drama
HBO has released the first full-length trailer for Sorkin's new Jeff Daniels starrer The Newsroom, which explores the inner-workings of a 24-hour cable news network.
The hourlong drama stars Daniels as Will, the Keith Olbermann of news anchors, and follows him as he becomes unraveled and does what ever he can to hold on to his audience.
Emily Mortimer, Alison Pill, John Gallgher Jr., Olivia Munn, Dev Patel, Thomas Sadoski and Sam Waterston star. Jane Fonda recurs as the CEO of the cable news network's parent company whose ideals often clash with the news outlet she oversees.
STORY: HBO Gives Series Order to Aaron Sorkin Project
"Progress is slow, but I'm in it for the long haul," Will says after expressing a string of less-than-popular political views.
Sorkin, who won an Academy Award for penning the screenplay forThe Social Network, will executive produce and write after spending time with high-profile cable news personalities like Olbermann and Chris Matthews to understand the world he planned to re-create.
Scott Rudin (The Social Network) and Alan Poul (Six Feet Under, The Big C) serve as executive producers, withGreg Mottola directing the pilot.
Newsroom becomes the latest in Sorkin's behind-the-scenes TV takes, following his exploration of a sports talk show in SportsNight and focus on sketch comedy in Studio 60 on the Sunset Strip.
Check out the promo below.
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Defconn is a popular South Korean hip hop singer and well-known television host. Born as Yoon Dae Joon on January 6, 1977, he is known for his mostly comical lyrics and his distinctive Afro-like hairstyle. Watch the music videos for his hit songs "King Wang Zzang" (featuring Heechul), "Rapper's Breakup" and "Stage of Friendship."