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J Hasek
Academy of Sciences of the Czech Republic, Praha, Hlavni mesto Praha, Czech Republic
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Available from: Michal Cifra
Conference Paper:
Two-channel measurement of the ultra-weak photon emission from a yeast culture during its growth
Katerina Cervinkova
Michaela Nerudova
Michal Cifra
, Jiri Hasek,
Jan Vrba
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The paper deals with a measurement of the spontaneous ultra-weak photon emission from wild type cells of yeast Saccharomyces cerevisiae during their growth. The objective of the work was to obtain a temporal development of optical signals from yeast samples utilizing two photomultiplier tubes with different properties.
2014 24th International Conference Radioelektronika (RADIOELEKTRONIKA); 04/2014
Available from: Mark Rinnerthaler
Mmi1, the yeast homologue of Mammalian TCTP, associates with stress granules in heat-shocked cells and modulates proteasome activity.
Mark Rinnerthaler
Renata Lejskova
Tomas Grousl
Vendula Stradalova
Gino Heeren
Klaus Richter
Lore Breitenbach-Koller
Jan Malinsky
, Jiri Hasek,
Michael Breitenbach
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As we have shown previously, yeast Mmi1 protein translocates from the cytoplasm to the outer surface of mitochondria when vegetatively growing yeast cells are exposed to oxidative stress. Here we analyzed the effect of heat stress on Mmi1 distribution. We performed domain analyses and found that binding of Mmi1 to mitochondria is mediated by its central alpha-helical domain (V-domain) under all conditions tested. In contrast, the isolated N-terminal flexible loop domain of the protein always displays nuclear localization. Using immunoelectron microscopy we confirmed re-location of Mmi1 to the nucleus and showed association of Mmi1 with intact and heat shock-altered mitochondria. We also show here that mmi1Δ mutant strains are resistant to robust heat shock with respect to clonogenicity of the cells. To elucidate this phenotype we found that the cytosolic Mmi1 holoprotein re-localized to the nucleus even in cells heat-shocked at 40°C. Upon robust heat shock at 46°C, Mmi1 partly co-localized with the proteasome marker Rpn1 in the nuclear region as well as with the cytoplasmic stress granules defined by Rpg1 (eIF3a). We co-localized Mmi1 also with Bre5, Ubp3 and Cdc48 which are involved in the protein de-ubiquitination machinery, protecting protein substrates from proteasomal degradation. A comparison of proteolytic activities of wild type and mmi1Δ cells revealed that Mmi1 appears to be an inhibitor of the proteasome. We conclude that one of the physiological functions of the multifunctional protein module, Mmi1, is likely in regulating degradation and/or protection of proteins thereby indirectly regulating the pathways leading to cell death in stressed cells.
PLoS ONE 10/2013; 8(10):e77791. DOI:10.1371/journal.pone.0077791
· 3.53 Impact Factor
Available from: Petr Pompach
Heat Shock-Induced Accumulation of Translation Elongation and Termination Factors Precedes Assembly of Stress Granules in S. cerevisiae.
Tomas Grousl
Pavel Ivanov
Ivana Malcova
Petr Pompach
Ivana Frydlova
Renata Slaba
Lenka Senohrabkova
Lenka Novakova
, Jiri Hasek
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In response to severe environmental stresses eukaryotic cells shut down translation and accumulate components of the translational machinery in stress granules (SGs). Since they contain mainly mRNA, translation initiation factors and 40S ribosomal subunits, they have been referred to as dominant accumulations of stalled translation preinitiation complexes. Here we present evidence that the robust heat shock-induced SGs of also contain translation elongation factors eEF3 (Yef3p) and eEF1Bγ2 (Tef4p) as well as translation termination factors eRF1 (Sup45p) and eRF3 (Sup35p). Despite the presence of the yeast prion protein Sup35 in heat shock-induced SGs, we found out that its prion-like domain is not involved in the SGs assembly. Factors eEF3, eEF1Bγ2 and eRF1 were accumulated and co-localized with Dcp2 foci even upon a milder heat shock at 42°C independently of P-bodies scaffolding proteins. We also show that eEF3 accumulations at 42°C determine sites of the genuine SGs assembly at 46°C. We suggest that identification of translation elongation and termination factors in SGs might help to understand the mechanism of the eIF2α factor phosphorylation-independent repression of translation and SGs assembly.
PLoS ONE 02/2013; 8(2):e57083. DOI:10.1371/journal.pone.0057083
· 3.53 Impact Factor
Available from: Naseem Gaur
Vps Factors are Required for Efficient Transcription Elongation in Budding Yeast.
Naseem A Gaur
, Jiri Hasek,
Donna Garvey Brickner
Hongfang Qiu
Fan Zhang
Chi-Ming Wong
Ivana Malcova
Pavla Vasicova
Jason H Brickner
Alan Hinnebusch
[Show abstract]
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There is increasing evidence that certain Vps proteins, factors that mediate vesicular protein trafficking, have additional roles in regulating transcription factors at the endosome. We found that yeast mutants lacking the PI(3)P kinase Vps34 or its associated protein kinase Vps15 display multiple phenotypes indicating impaired transcription elongation. These phenotypes include reduced mRNA production from long or G+C-rich coding sequences (CDS) without affecting the associated GAL1 promoter activity, and a reduced rate of RNA polymerase II (Pol II) progression through lacZ CDS in vivo. Consistent with reported genetic interactions with mutations affecting the histone acetyltransferase complex NuA4, vps15Δ and vps34Δ mutations reduce NuA4 occupancy in certain transcribed CDS. vps15Δ and vps34Δ mutants also exhibit impaired localization of the induced GAL1 gene to the nuclear periphery. We found unexpectedly that, similar to known transcription elongation factors, these and several other Vps factors can be cross-linked to the CDS of genes induced by Gcn4 or Gal4 in a manner dependent on transcriptional induction and stimulated by Cdk7/Kin28-dependent phosphorylation of the Pol II CTD. We also observed co-localization of a fraction of Vps15-GFP and Vps34-GFP with nuclear pores at nucleus-vacuole (NV) junctions in live cells. These findings suggest that Vps factors enhance the efficiency of transcription elongation in a manner involving their physical proximity to nuclear pores and transcribed chromatin.
Genetics 01/2013; DOI:10.1534/genetics.112.146308
· 4.87 Impact Factor
Available from: Pavla Vasicova
Nuclear import of chromatin remodeler Isw1 is mediated by atypical bipartite cNLS and classical import pathway.
Pavla Vasicova
Vendula Stradalova
Petr Halada
, Jiri Hasek,
Ivana Malcova
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The protein Isw1 of Saccharomyces cerevisiae is an imitation-switch chromatin-remodeling factor. We studied the mechanisms of its nuclear import and found that the nuclear localization signal (NLS) mediating the transport of Isw1 into the nucleus is located at the end of the C-terminus of the protein (aa1079-1105). We show that it is an atypical bipartite signal with an unconventional linker of 19 aa (KRIR X(19) KKAK) and the only nuclear targeting signal within the Isw1 molecule. The efficiency of Isw1 nuclear import was found to be modulated by changes to the amino-acid composition in the vicinity of the KRIR motif, but not by the linker length. Live-cell imaging of various karyopherin mutants and in vitro binding assays of Isw1NLS to importin-α revealed that the nuclear translocation of Isw1 is mediated by the classical import pathway. Analogous motifs to Isw1NLS are highly conserved in Isw1 homologues of other yeast species, and putative bipartite cNLS were identified in silico at the end of the C-termini of ISWI proteins from higher eukaryotes. We suggest that the C-termini of the ISWI family proteins play an important role in their nuclear import.
Traffic 11/2012; DOI:10.1111/tra.12025
· 4.71 Impact Factor
Available from: Thomas Klaus Felder
Yno1p/Aim14p, a NADPH-oxidase ortholog, controls extramitochondrial reactive oxygen species generation, apoptosis, and actin cable formation in yeast.
Mark Rinnerthaler
Sabrina Büttner
Peter Laun
Gino Heeren
Thomas K Felder
Harald Klinger
Martin Weinberger
Klaus Stolze
Tomas Grousl
, Jiri Hasek, [......],
Ivana Frydlova
Andrea Klocker
Birgit Simon-Nobbe
Bettina Jansko
Hannelore Breitenbach-Koller
Tobias Eisenberg
Campbell W Gourlay
Frank Madeo
William C Burhans
Michael Breitenbach
[Show abstract]
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The large protein superfamily of NADPH oxidases (NOX enzymes) is found in members of all eukaryotic kingdoms: animals, plants, fungi, and protists. The physiological functions of these NOX enzymes range from defense to specialized oxidative biosynthesis and to signaling. In filamentous fungi, NOX enzymes are involved in signaling cell differentiation, in particular in the formation of fruiting bodies. On the basis of bioinformatics analysis, until now it was believed that the genomes of unicellular fungi like Saccharomyces cerevisiae and Schizosaccharomyces pombe do not harbor genes coding for NOX enzymes. Nevertheless, the genome of S. cerevisiae contains nine ORFs showing sequence similarity to the catalytic subunits of mammalian NOX enzymes, only some of which have been functionally assigned as ferric reductases involved in iron ion transport. Here we show that one of the nine ORFs (YGL160W, AIM14) encodes a genuine NADPH oxidase, which is located in the endoplasmic reticulum (ER) and produces superoxide in a NADPH-dependent fashion. We renamed this ORF YNO1 (yeast NADPH oxidase 1). Overexpression of YNO1 causes YCA1-dependent apoptosis, whereas deletion of the gene makes cells less sensitive to apoptotic stimuli. Several independent lines of evidence point to regulation of the actin cytoskeleton by reactive oxygen species (ROS) produced by Yno1p.
Proceedings of the National Academy of Sciences 05/2012; 109(22):8658-63. DOI:10.1073/pnas.1201629109
· 9.81 Impact Factor
Available from: Pavel Ivanov
Robust heat shock induces eIF2 alpha-phosphorylation-independent assembly of stress granules containing eIF3 and 40S ribosomal subunits in budding yeast, Saccharomyces cerevisiae
Tomás Grousl
Pavel Ivanov
Ivana Frýdlová
Pavla Vasicová
Filip Janda
Jana Vojtová
Katerina Malínská
Ivana Malcová
Lenka Nováková
Dana Janosková
Leos Valásek
, Jirí Hasek
[Show abstract]
[Hide abstract]
Environmental stresses inducing translation arrest are accompanied by the deposition of translational components into stress granules (SGs) serving as mRNA triage sites. It has recently been reported that, in Saccharomyces cerevisiae, formation of SGs occurs as a result of a prolonged glucose starvation. However, these SGs did not contain eIF3, one of hallmarks of mammalian SGs. We have analyzed the effect of robust heat shock on distribution of eIF3a/Tif32p/Rpg1p and showed that it results in the formation of eIF3a accumulations containing other eIF3 subunits, known yeast SG components and small but not large ribosomal subunits and eIF2alpha/Sui2p. Interestingly, under these conditions, Dcp2p and Dhh1p P-body markers also colocalized with eIF3a. Microscopic analyses of the edc3Deltalsm4DeltaC mutant demonstrated that different scaffolding proteins are required to induce SGs upon robust heat shock as opposed to glucose deprivation. Even though eIF2alpha became phosphorylated under these stress conditions, the decrease in polysomes and formation of SGs occurred independently of phosphorylation of eIF2alpha. We conclude that under specific stress conditions, such as robust heat shock, yeast SGs do contain eIF3 and 40S ribosomes and utilize alternative routes for their assembly.
Journal of Cell Science 07/2009; 122(Pt 12):2078-88. DOI:10.1242/jcs.045104
· 5.33 Impact Factor
Available from: Ivana Malcova-Janatova
Deregulation of DSE1 gene expression results in aberrant budding within the birth scar and cell wall integrity pathway activation in Saccharomyces cerevisiae.
Ivana Frydlová
Ivana Malcová
Pavla Vasicová
, Jirí Hasek
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[Hide abstract]
Strains of Saccharomyces cerevisiae lacking Isw2, the catalytic subunit of the Isw2 chromatin remodeling complex, show the mating type-independent activation of the cell wall integrity (CWI) signaling pathway. Since the CWI pathway activation usually reflects cell wall defects, we searched for the cell wall-related genes changed in expression. The genes DSE1, CTS1, and CHS1 were upregulated as a result of the absence of Isw2, according to previously published gene expression profiles (I. Frydlova, M. Basler, P. Vasicova, I. Malcova, and J. Hasek, Curr. Genet. 52:87-95, 2007). Western blot analyses of double deletion mutants, however, did not indicate the contribution of the chitin metabolism-related genes CTS1 and CHS1 to the CWI pathway activation. Nevertheless, the deletion of the DSE1 gene encoding a daughter cell-specific protein with unknown function suppressed CWI pathway activation in isw2Delta cells. In addition, the deletion of DSE1 also abolished the budding-within-the-birth-scar phenotype of isw2Delta cells. The plasmid-driven overexpression proved that the deregulation of Dse1 synthesis was also responsible for CWI pathway activation and manifestation of the budding-within-the-birth-scar phenotype in wild-type cells. The overproduced Dse1-green fluorescent protein localized to both sides of the septum and persisted in unbudded cells. Although the exact cellular role of this daughter cell-specific protein has to be elucidated, our data point to the involvement of Dse1 in bud site selection in haploid cells.
Eukaryotic Cell 03/2009; 8(4):586-94. DOI:10.1128/EC.00376-08
· 3.18 Impact Factor
Measurement of electrical oscillations and mechanical vibrations of yeast cells membrane around 1 kHz.
Frantisek Jelínek
Michal Cifra
Jirí Pokorný
Jan Vanis
Jan Simsa
, Jirí Hasek,
Ivana Frýdlová
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Fröhlich postulated coherent polar oscillations as a fundamental biophysical property of biological systems. Recently, Pelling et al. (2004, 2005) detected mechanical vibrations of yeast cell membrane with atomic force microscope (AFM) and analyzed by Fourier analysis in the frequency range 0.5-2 kHz with amplitudes of the order of 1 nm. This article describes the measurement of electric activity of yeast cells in the acoustic frequency range and of mechanical vibrations of cell membrane. Spectrum analyzer and electrically and electromagnetically screened box with point sensor and amplifiers fed by batteries were used for measurement of synchronized and non synchronized tubulin mutants of yeast cells. We show that the electric activity of synchronized cells in the M phase is greater that of non synchronized cells. That corresponds to the findings of Pohl et al. (1981). Obtained results of measurement of cell electric activity are in good agreement with AFM findings.
Electromagnetic Biology and Medicine 01/2009; 28(2):223-32. DOI:10.1080/15368370802710807
· 0.77 Impact Factor
Available from: Fan Zhang
Disrupting vesicular trafficking at the endosome attenuates transcriptional activation by Gcn4.
Fan Zhang
Naseem A Gaur
, Jiri Hasek,
Soon-ja Kim
Hongfang Qiu
Mark J Swanson
Alan G Hinnebusch
[Show abstract]
[Hide abstract]
The late endosome (MVB) plays a key role in coordinating vesicular transport of proteins between the Golgi complex, vacuole/lysosome, and plasma membrane. We found that deleting multiple genes involved in vesicle fusion at the MVB (class C/D vps mutations) impairs transcriptional activation by Gcn4, a global regulator of amino acid biosynthetic genes, by decreasing the ability of chromatin-bound Gcn4 to stimulate preinitiation complex assembly at the promoter. The functions of hybrid activators with Gal4 or VP16 activation domains are diminished in class D mutants as well, suggesting a broader defect in activation. Class E vps mutations, which impair protein sorting at the MVB, also decrease activation by Gcn4, provided they elicit rapid proteolysis of MVB cargo proteins in the aberrant late endosome. By contrast, specifically impairing endocytic trafficking from the plasma membrane, or vesicular transport to the vacuole, has a smaller effect on Gcn4 function. Thus, it appears that decreasing cargo proteins in the MVB through impaired delivery or enhanced degradation, and not merely the failure to transport cargo properly to the vacuole or downregulate plasma membrane proteins by endocytosis, is required to attenuate substantially transcriptional activation by Gcn4.
Molecular and Cellular Biology 10/2008; 28(22):6796-818. DOI:10.1128/MCB.00800-08
· 5.04 Impact Factor
Available from: Jan Vanis
Biophysical aspects of cancer--electromagnetic mechanism.
J Pokorný
, J Hasek,
J Vanis
F Jelínek
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[Hide abstract]
Hypothesis of coherent vibration states in biological systems based on nonlinear interaction between longitudinal elastic and electric polarization fields with metabolic energy supply was formulated by Frohlich. Conditions for excitation of coherent states and generation of electromagnetic fields are satisfied in microtubules which form electrical polar structures. Numerical models are used for analysis of Frohlich's vibration states in cells. Reduction of activity and of energy production in mitochondria, and disintegration of cytoskeleton structures by phosphorylation on the pathway of cancer trasformation can diminish excitation of the Frohlich's vibration states and of the generated electromagnetic field, which results in disturbances of the interaction forces between cells. Interaction forces between cancer cells may be smaller than interaction forces between healthy cells and cancer cells as follows from numerical models. Mechanism of malignity, i.e. local invasion, detachment of cancer cells, and metastasis, is assumed to depend on the electromagnetic field.
Indian journal of experimental biology 06/2008; 46(5):310-21.
· 0.75 Impact Factor
Conference Paper:
Ultra Low Frequency Yeast Cells Electric Activity
M. Cifra
J. Pokorny
F. Jelinek
, J. Hasek,
J. Simsa
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This paper presents results from the measurement of the electrical activity cold sensitive beta-tubulin mutant tub2-401 of yeast cells in frequency region 0.4 - 1.6 kHz. 25 measurements of both synchronized and nonsynchronized cells show that the synchronized cells have higher electrical activity which corresponds to the findings of Pohl et al. Local nanomechanical vibrations of yeast cell membrane have been measured by Pelling et al. with Atomic Force Microscopy in same frequency region. Mechanical oscillations of electrically polar structures in cell may give rise to electric oscillations we measured.
Microwave Techniques, 2008. COMITE 2008. 14th Conference on; 05/2008
Conference Paper:
Measurement of Electrical and Mechanical Oscillations of Yeast Cells Membrane in Acoustic Frequency Range
F. Jelinek
J. Pokorny
J. Vanis
, J. Hasek,
J. Simsa
[Show abstract]
[Hide abstract]
Frohlich postulated coherent polar oscillations as a fundamental biophysical property of biological systems. Pelling et al. detected recently mechanical vibrations of yeast cell membrane in the frequency range 0.5-2 kHz with atomic force microscope (AFM). This paper describes the measurement of electric activity of yeast cells at frequencies around 1 kHz and of mechanical vibrations of cell membrane. Synchronized and non- synchronized tubulin mutants of yeast cells were measured. The paper includes a description of the laboratory equipment with a platinum wire point sensor and a spectrum analyzer. Obtained results are in good agreement with AFM findings.
Microwave Techniques, 2008. COMITE 2008. 14th Conference on; 05/2008
Available from: Ivana Malcova-Janatova
Special type of pheromone-induced invasive growth in Saccharomyces cerevisiae.
Ivana Frýdlová
Marek Basler
Pavla Vasicová
Ivana Malcová
, Jirí Hasek
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[Hide abstract]
The ability to invade a solid substrate is an important phenomenon due to its connection with pathogenic activity of fungi. We report here on invasion displayed by MATalpha cells of Saccharomyces cerevisiae lacking Isw2p, a subunit of the ISW2 chromatin remodelling complex. We found that on minimal medium, where the isw2Delta MATalpha mutant is not invasive, additional absence of another ISW2 complex subunit, Dls1p or Dpb4p, promoted invasion. Our microarray data showed that derepression of MAT a-specific genes caused by absence of Isw2p is very low. Their expression is increased only by the autocrine activation of the mating pathway. Invasion of isw2Delta MATalpha cells thus resembles the pheromone-induced invasion, including dependence on Fig2p. We show here that another pheromone-induced protein, mating agglutinin Aga1p, can play a role in the agar adhesion necessary for invasion. In contrast with MAT a-cells invading agar under low alpha-pheromone concentration, the invasive growth of isw2Delta cells specifically requires Fus3 kinase. Its function in the invasion of isw2Delta MATalpha cells cannot be completely substituted by Kss1 kinase, which plays a basic role in invasive growth signalling. We suggest that partial dependence of the isw2Delta MATalpha invasion on Fus3p and Aga1p corresponds to a weaker pheromone response of this mutant.
Current Genetics 09/2007; 52(2):87-95. DOI:10.1007/s00294-007-0141-2
· 1.71 Impact Factor
Available from: Michal Cifra
Electrical Vibrations of Yeast Cell Membrane
Michal Cifra
J. Vanis
O. Kucera
, Jiri Hasek,
Ivana Frydlova
Frantisek Jelinek
Jaroslav Saroch
Jiri Pokorny
PIERS Online 01/2007; 3(8):1190-1194. DOI:10.2529/PIERS070220101159
Available from: Nicole Jaffrezic-Renault
Measurement of Electromagnetic Activity of Yeast Cells at 42 GHz
Jelinek F
Saroch J
Kucera O
, Hasek J,
Pokorny J
Jaffrezic-Renault N
Ponsonnet L
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[Hide abstract]
This paper discusses the possibility of using a device composed of a resonant cavity, preamplifiers, and a spectrum analyzer to detect electromagnetic emission of yeast cells at a frequency of about 42 GHz. Measurement in this frequency range is based on the Frohlich's postulate of coherent polar oscillations as a fundamental biophysical property of biological systems and on the experiments of Grundler and Keilmann who disclosed effects of exposure to the electromagnetic field at 42 GHz on the growth rate of yeast cells. This article includes a detailed description of the laboratory equipment and the methods used to evaluate the obtained results.
Available from: Oldřich Benada
The WD-40 repeat protein PkwA of Thermomonospora curvata is associated with rapid growth and is localized in the tips of growing hyphae
Katerina Petrícková
, Jirí Hasek,
Oldrich Benada
Miroslav Petrícek
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[Hide abstract]
The PkwA protein of the thermophilic actinomycete Thermomonospora curvata has already been reported as the first instance of a WD-40 module-containing protein of prokaryotic origin. This protein is composed of an N-terminal eukaryotic-type protein kinase domain and of seven C-terminal WD-40 repeats. PkwA is a peripheral membrane protein that is linked to the early exponential growth phase of the bacterium. Its intracellular concentrations are extremely low. We have shown that the protein forms high molecular weight complexes and is localized mainly in the tips of the young Thermomonospora vegetative hyphae.
FEMS Microbiology Letters 06/2006; 258(2):187-93. DOI:10.1111/j.1574-6968.2006.00215.x
· 2.72 Impact Factor
Yeast fluorescence microscopy.
Jirí Hasek
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Fluorescence microscopy is the essential technique for investigation of the intracellular distribution of macromolecules and various organelles also in yeast cells. In this chapter, detailed practical procedures for fluorescence microscopic observations developed or adopted in our laboratory are described. These include labeling of the cell wall and chitin, F-actin structures, nuclear and mitochondrial DNA, and two different procedures for investigation of yeast cells by immunofluorescence. In addition, our experience with multicolor labeling experiments is introduced and discussed.
Methods in Molecular Biology 02/2006; 313:85-96. DOI:10.1385/1-59259-958-3:085
· 1.29 Impact Factor
Available from: onlinelibrary.wiley.com
Interaction of Pik1p and Sjl proteins in membrane trafficking
Peter H Nguyen
, Jiri Hasek,
Sepp D Kohlwein
Carlos Romero
Jae H Choi
Ales Vancura
[Show abstract]
[Hide abstract]
Phosphatidylinositol (PtdIns) phosphates are involved in signal transduction, cytoskeletal organization, and membrane traffic. PtdIns 4-phosphate [PtdIns(4)P], produced in yeast by PtdIns 4-kinase (Pik1p), appears to regulate Golgi secretory function. PtdIns(4)P is also produced by dephosphorylation of phosphatidylinositol 4,5-bisphosphate [PtdIns(4,5)P2], catalyzed by one of the three yeast Sjl proteins, homologs of the mammalian synaptic vesicle-associated PtdIns(4,5)P2 5-phosphatase, synaptojanin. To determine whether Pik1p and Sjl proteins operate in the same pathway or regulate the same process, we used a genetic approach. Mutation in the PIK1 gene displays synthetic genetic interactions with deletions of individual SJL genes. Deletion of SJL3 gene is synthetically lethal with pik1ts, and deletions of SJL1 or SJL2 genes in pik1ts cells exacerbate the temperature sensitivity, neomycin sensitivity, and defect in invertase secretion. A diminished level of PtdIns(4)P and increased level of PtdIns(4,5)P2 in pik1(ts)sjl1delta and pik1(ts)sjl2delta cells, compared with pik1ts cells, indicate that PtdIns(4)P is specifically required for secretion. Collectively, our results suggest that Pik1p and the Sjl proteins coordinately function to regulate the dynamic phosphorylation-dephosphorylation of the polar heads of phosphoinositides, and this process appears to be important for membrane trafficking pathways.
FEMS Yeast Research 03/2005; 5(4-5):363-71. DOI:10.1016/j.femsyr.2004.09.007
· 2.44 Impact Factor
Available from: chir3.lf1.cuni.cz
Specification of the monoclonal antibody PK1 reactivity in Chinese hamster ovary cells.
J Hasek,
L Perinka
L Valásek
Folia biologica 02/2005; 51(2):50-1.
· 0.78 Impact Factor
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Ivana Malcova-Janatova
Academy of Sciences of the Czech Republic
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Pavla Vasicova
Academy of Sciences of the Czech Republic
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Jan Vanis
Academy of Sciences of the Czech Republic
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Michal Cifra
Institute of Photonics and Electronics (IPE)
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Alan G Hinnebusch
National Institutes of Health
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text:
Well, I wanted to have a good canidate for Bourbon Oak Chip aging and this seemed like the perfect base for it. I got this out of the Extreme Brewing book but for some reason I feel like there's something missing (also, I'm just going to use regular chocolate malt insted of "light").
Dogfish Head Indian Brown Ale
7 lbs Light Dry Malt Extract
10 oz British Amber Malt
10 oz 60 Lovibond Crystal Malt
8 oz Light chocolate Malt
2 oz Roasted Barley
½ oz Warrior hops (60 min)
8 oz Dark Brown Sugar (10 min)
1 oz Vanguard hops (end of boil)
Ringwood Ale/Wyeast 1187
Brewer for Hoppin' Frog Brewery.
"I am not an alcoholic, Im a drunk. An alcoholic means that I have a problem and when I drink all my problems go away."
I made this recipe last June and was unimpressed. It is nowhere close to the real thing. Mine was VERY roasty, almost salty, and one dimensional. The malt bill is wrong. If I were to do it again, I'd:
- Try to exchange the hops bill for what they actually say on the bottle (can't recall but I know it is different).
- exchange the Crystal 60L for 8 oz Special B,
- Might add a touch of Munich (1lb or less) and offset the LDME.
I very much liked the Ringwood yeast, but I don't think that's what they used in production. Just some thoughts, but if someone has a good recipe, I'd be interested to see it.
Awwww....bummer. I was hoping this would been a good one....but my Brewer-sense was tingling (either that or the Head and Soulders shampoo was working) so I knew something was off about the recipe.
Well, it looks like I'll just fall back to my original plan which was this:
Bell's Best Brown Ale (5 gallons, extract with grains)
OG = 1.058 FG = 1.013 IBUs = 30 ABV = 5.9%
6 lbs. Light malt extract
14 oz. Victory malt
14 oz. Special Roast malt
14 oz. Crystal malt (60° L)
2 oz. Chocolate malt
6.2 AAU Cascade hops (bittering hop)
(0.75 oz. of 8.3% alpha acid)
3.25 AAU Nugget hops (bittering hop)
(0.25 oz. of 13% alpha acid)
1.2 AAU Fuggle hops (flavor hop)
(0.25 oz. of 4.7% alpha acid)
2.4 AAU Fuggle hops (aroma hop)
(0.5 oz. of 4.7% alpha acid)
1 tsp. Irish moss for 45 min.
White Labs WLP001 (California Ale) yeast
Me and some buddies have tried multiple times to clone Indian Brown with not so great results. My one buddy made the recipe from the book to a T, and it definately got better with age. Like 6-12 months of age. Even then, it didn't taste too similar to the real thing, but it was good.
Mine was too grassy from the dry hops, but I did let them sit in the fermenter for 2 weeks. Also, the original recipe calls for coffee malt, which I couldn't find locally. They say light chocolate is a good substitute, but I couldn't find that either. I ended up with regular chocolate malt too.
I think your Bell's clone looks good. This thread got me thinking, so I bought a 6pack of Indian Brown Ale today. Hops are Liberty and Goldings as written on the bottle, which are different that the Vanguard and Warrior in the recipe. I'm going to try a tasting and see what comes to mind - I've wanted to clone this beer, and have been unsuccessful in finding anything on the internet except the book's recipe.

subset: commoncrawl


text:
iittala Sarjaton 10" Metsa Dinner Plate
iittala Sarjaton 10" Metsa Dinner Plate
Weights & Dimensions
|Product Type: Dinner plate
|Style: Modern
|Color: White
|Material: Porcelain
|Number of Items Included: 1
|Lead-Free: Yes
|Formal: Yes
|Shape: Round
|Pattern: Solid color
|Oven Safe: Yes
|Stove Safe: No
|Microwave Safe: Yes
|Dishwasher Safe: Yes
|Refrigerator Safe: Yes
|Freezer Safe: Yes
|Food Safe: Yes
|Stackable: Yes
|Commercial Use: Yes
|Country of Manufacture: Finland
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iittala Sarjaton 10" Metsa Dinner Plate
About the ManufacturerIn the quiet north, there is a country that specializes in aesthetics and functional design. In Finland, in a town called Iittala, style and high quality are traditions that remain relevant for generations to come. Whenever an object is produced in the iittala factory, it has to feel, last and work like iittala. The tactility and high quality of the materials can be felt with your bare hands. Objects like that, will work anytime, anywhere with anything. Objects like that will never be thrown away.
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Modern design with a great patern and color
Mark from Indianapolis, IN– Verified Buyer
The collection allows you to create your personal dinner set. The simple design and patterns, along with great colors and durability make these a timeless everyday addition.
OUR SKU # IIT2173Sarjaton 10" Metsa Dinner Plate is also sometimes listed under the following manufacturer numbers: SAR016810


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Hey Guys i have rooted my nokia x and now google play store running well on my device on the latest sowftware ver 188.8.131.52
now if i updated my software , that means i will lost my root ?????
Yes, you might loose root. But you'll be able to root again using Nokia X Manager.
However, if by update, you mean installing custom ROMs, then let me tell you that almost all of them are rooted.
XDA Developers was founded by developers, for developers. It is now a valuable resource for people who want to make the most of their mobile devices, from customizing the look and feel to adding new functionality.
Are you a developer?
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Learn how MacUpdate Desktop can install apps on MacUpdate with the simple click of the icon. Plus, keep all your apps updated.
MailMate is an ambitious, powerful email client for OS X. It is designed for and only supports IMAP while still working in full when offline. MailMate has state-of-the-art searching capabilities and correspondingly advanced smart mailboxes. Extensive keyboard control and dynamic signatures ensure a highly efficient workflow when reading and answering emails. Alternative message viewer layouts and a browser-inspired design ensure easy and rapid access to related messages.
- Universal mailboxes: One Inbox for all of your IMAP accounts.
- Extensive keyboard control: Switch mailboxes or move more...
What's New
Note: Now requires OS X 10.7 or later running on a 64-bit Intel processor.
- Bundles preferences pane which allows you to enable/disable integration with various applications. Currently, 19 bundles exist providing support for BBEdit, BusyCal, Due, EagleFiler, Evernote, OmniFocus, TextMate, Todoist, and more.
- Rules pane in the mailbox editor for any more...
- OS X 10.7 or later
- IMAP email accounts


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Your welcome! Yeah I am spending with his family and mine to I am just staying with him the whole time. Lol yeah I understand that I too have a short attention span lol. If an anime don't interest me I just tune out and just forget it and look for something ealse to watch.
I'll be okay. Thanks You're so lucky. I just have to spend Christmas with my family. I've watched a little Sailor Moon and Yu-Gi-Oh, but the others I haven't. I have a short attention span, so the really really long shows just don't interest me.
Oh sorry to hear that I hope you get well soon! I am good thanks I am looking foreword to Christmas brake! I am spending it with my bf Roger. Well my fave anime would be Naruto. Other then that I like romance anime. Anime that I am into so far would be naruto-bleach-death note-inuyasha-vampire knight-pokemon-sailor moon-Yugioh-Yu Yu Hakusho-Kenichi and some others.
I'm okay. I'm off school sick though and am bored. How are you? I mostly like action-y and comedy shows like Rune Soldier and Getbackers, among others. What about you, what are you into?
Hello Kimiko! How are you today? What kind of anime are you into? anyways I hope we can be friends.


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Jane has been a nice quiet guest and would love to have her back and hopefully next time will be with a little less noise which seems to have bothered her a bit but one gets used to it.
December is a noisy month with the celebration of various saints, fireworks and music as it always is this time of year but I like to think of it as "happy sound".
Fui muy afortunada de conocer a Jane. Su visita fue muy corta pero pudimos observar lo agradable que es conocer a personas como ella, sencilla, simpática, atenta y responsable. Nos encantaría volver a tenerla con nosotros.
Jane ha sido una excelente huesped, muy ocupada, tuve oportunidad de convivir con ella y de platicar sobre diversos temas, definitivamente es una huesped absolutamente recomendable y siempre será bienvenida en Palo Mulato.
Jane was a fine guest. She appreciated the accommodations and got a good night's sleep.
Jane is so cheerful, interesting and full of life. She came early enough (daylight) to get a bit of a tour of the property and meet the hens, she she took pictures of. She was well pleased to enjoy an evening fire, and in the morning, a cup of tea in the kitchen with the sun shining in. The house was left very neat.
Jane was great fun to have with us. It is nice to meet someone you can share that shares similarities with and to hear about her past and future adventure. Love to have her visit again!


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Cayenne River
From Wikipedia, the free encyclopedia
|This article does not cite any references or sources. (December 2009)
The Cayenne River is a 50 km-long river in French Guiana. Formed by the Cascades River and the Tonnégrande River, it flows into the Atlantic Ocean near the city of Cayenne, forming a large estuary, about 2 km long.
The river was prominently featured in both the movie and book Papillon by Henri Charrière.
|This French Guiana (Guyane) location article is a stub. You can help Wikipedia by expanding it.


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Post by Judy Dutton. Where do celebrities go for parenting advice? Well, if you ask Cindy Crawford, Glee producer Ryan Murphy, The Talk host Julie Chen, and many others in the Hollywood area, they'd swear by baby nurse Luiza DeSouza....Show More Summary
Cindy Crawford keeps getting hotter and hotter! The supermodel queen of the 90s was spotted doing a sensual photo shoot on the beach in Malibu on Sunday. [ Related: Cindy Crawford Takes Legal Action Against A Photoshopper ] She wore nothing but black panties under a sheer white mesh dress, and her boobs were completely visible! They are seriously [...]
Something tells me Mattel is creating another collector's item for their Barbie collection. Supermodel Cindy Crawford got her very own Barbie doll, and she's wearing designer Roberto Cavalli! RELATED: Cindy Crawford says unretouched photo was actually stolen and photoshopped. Cindy shared a picture of her doll in Instagram. Thanks @mattel for my very own @robertocavalli […]
Cindy Crawford recently shared a bit of wisdom with her fans on Instagram: "Love keeps us young!" She even proved her point by posting the perfect photo.The picture features Crawford's mother, Jennifer, moments after saying "I do" to her new husband.Crawford also offered a personal message to the couple, writing, "Congrats mom and Charlie!!! ? #newlyweds.
Model Bar Rafaeli has been gracing ad and magazine pages for seemingly forever, but the Israeli-born model is only 29. Since first appearing (...)
The legendary beauty's 13-year-old daughter looks just like her gorgeous mom (not that we're jealous or anything).
Who says that growing older means having to put away that sexy little swimsuit for good? Check out RadarOnline.com's gallery of 20 sexy stars who are growing older — and… READ ON
Plus-size model Tess Holliday is the latest woman to go Photoshop-free in her new campaign for retailer Torrid, a fashion line for sizes 12 to 28. But unlike Beyoncé and Cindy Crawford, Holliday purposely avoided Photoshop for the new ads. Show More Summary
Supermodel Christie Brinkley is sharing all her sexy-at-61 secrets. From diet to anti-aging tips, Brinkley is ready to tell all, reported Fox News. Talking with Dr. Mehmet Oz on his talk show, Christie also discussed the controversy that ensued when Cindy Crawford was revealed looking fabulous at 49 in lingerie. Show More Summary
Photographer John Russo today works and plays a long way from Ventnor, New Jersey. But as far as that famous \"leaked\" photo of Cindy Crawford from a 2013 December Marie Claire cover shoot is concerned, he wants everybody to know that...Show More Summary
So much for letting us believe that Crawford and her superior genes age like the rest of us.
Post by Liz Alterman. Remember those photos of Cindy Crawford that surfaced a few weeks ago? You know, the ones that made every woman feel better about her less-than-perfect stomach and upper thighs? Well, as much as we all might want...Show More Summary
Jessie James Decker announces second pregnancy, Melanie Griffith and Don Johnson visit Dakota Johnson on 'SNL,' Kate Middleton shows growing baby bump, and more celebrity news.
You betta work... on those C&D letters! The viral picture of Cindy Crawford's "unretouched" midsection is allegedly fake, and a lawyer for the photographer who took the original picture is threatening publishers with legal action if the supposedly doctored photos aren't taken down immediately. Show More Summary
Madonna, Beyoncé, and Cindy Crawford have been consistently mocked for supposed "unretouched" photos that make them look horrible. However, many are alleging that "unretouched" photos of Madonna and the other gals are Photoshopped to make them look worse. Show More Summary
Plus Cindy Crawford is not at all like us and Kim and Kanye have ruined Fashion Week for babies everywhere.
Last month, an "unphotoshopped" pic of Cindy Crawford leaked on Twitter and instantly went viral. The photo was allegedly an un-retouched original from an ad campaign the iconic model posed for in 2013. As the pic circulated on social...Show More Summary
Britney Spears' Weave Falls Out During A Show & She Doesn't Miss A Beat! Watch! [CLICK HERE] Cindy Crawford Is Taking Legal Action Against The Person Who Altered Her Viral 'Unretouched' Photo! [CLICK HERE] ?Girl Uses A Lip Plumping Gadget & Gets WAY More Than She Bargained For! [CLICK HERE] Nikki Reed & Ian Somerhalder Only Fight Over ONE Thing! Can [...]
Cindy Crawford has no trouble pushing 50 with her perfectly toned body. Photoshoppers HATE her! (via Rande Gerber) Remember a few weeks ago when we thought that supermodel Cindy Crawford was a human bound by the same laws of aging and...Show More Summary
In a case of "Don't believe everything you read on the Internet," those supposedly unretouched photos of Cindy Crawford in a bikini might have been retouched after all—to make her look worse. Read more...


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Kaytee Igloo - Assorted Colors
|Kaytee Animal Igloo comes in assorted colors and they're made of translucent plastic so you can see your pet inside its Igloo even when your pet thinks its hiding! Super Pet Igloos are constructed from durable plastic making them easy to clean, plus they're odor and stain resistant. Secure resting spot Made with durable plastic that's easy to clean Cool hideout with air vents Igloo's one piece design is easy to use and every Igloo has fresh air vents to ensure your pets health and well-being. Igloos make a "cool" hideout for guinea pigs, ferrets, dwarf rabbits, chinchillas and other furry friends.Comes in assorted colors -- please allow us to select one for you.
Kaytee Natural Timothy Hay
|Kaytee Natural Timothy Hay is a natural product with no preservatives or additives, and because timothy hay is lower in calcium, it may decrease the likelihood of urinary tract problems. For chinchillas, guinea pigs, rabbits, and other small animals. Nutritionally fortified Natural sun-cured timothy hay For small animals Timothy hay is a natural product and as such is subject to the capricious whims of mother nature. It can range in color from green to a yellowish brown - but do not fret! Both colors are nutritionally sound. In fact, brown hay is slightly higher in fiber than green, but this is the only difference. Since sun, rain and other weather conditions affect the color of the hay crop, Pet Mountain cannot guarantee one color or the other.Ingredients: Natural sun-cured timothy hayGuaranteed Analysis:Crude Protein (min.)..........6.0%Crude Fat (min.)..........2.0%Crude Fiber (max.).........32.0%Moisture (max.).........0.75%Calcium (min.)..........0.25%
CareFresh Complete Natural Paper Bedding for Small Pets
|CareFresh Complete Natural Paper Pet Bedding is the safest and healthiest bedding for your pet made from reclaimed pulp waste. This short fiber pulp is free of ink, dye, clay and other chemicals that can be used in the paper making process. 10 day odor control with Odor Stop baking soda formula 3X more absorbent than shavings 99% dust free for easy clean-up Chemical free pet bedding with no pine or cedar oils Compressed CareFresh Natural Bedding expands when removed from the packaging. For example 25.7 liter bag (1,571 cu. in.) expands to 60 liters (3,661 cu. in.). This natural pet bedding is also processed to remove harmful aromatic hydrocarbons that come in pine and cedar oils. This odor blocking bedding is made to suppress the formation of ammonia caused from pet urine. CareFresh Natural Pet Bedding is extremely absorbent, able to take on up to 3X its weight in fluids, including pet urine and water leaks! This bedding will last you twice as long as shavings, saving you time and money! Pet Mountain is proud to offer discount bulk pet products.Made in the USA.Old Packaging:60L UPC: 06638018025130L UPC: 066380004151


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Ariel Pink Brings "Round And Round" To Fallon
Ariel Pink's lack of live game's been in the news, but last night he got a band together and put his best skinny leg forward for a performance of "Round And Round" on Jimmy Fallon. It was his network television debut.
Before Today is out today 4AD.


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Michigan to get facelift
The two-mile Michigan International Speedway will be repaved after the Aug. 21 Pure Michigan 400. But unlike other projects on the circuit, drivers seem to approve of the facelift.
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Thursday Commentary
8:00 am
Thu April 12, 2012
The Fact Behind the Facts
As a budding journalist with his first front-page byline, Commentator Philip Gerard was understandably smug.
The piece he'd written, on a young man who saved his girlfriend from a burning car, had won him recognition--professionally and locally. But, years later, when one local gives him an essential detail about the piece--one Philip had missed--he starts to wonder about the fact behind the facts, and how to reach it.


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Phil Waterford didn't want to see people go without during the holiday season.
Jewel. James Taylor. Jack Johnson. Lucia Barragain?
Editor's note: The following is part of a series recapping Manteca history as the first decade of the 21st century draws to a close.
It is now legal for larger tractor-trailer truck combinations that almost exclusively serve major distribution centers such as Millard Refrigeration, Ford Motor Co. and ADPS Packaging to access Spreckels Park.
MRPS Hall in Manteca will be the new meeting place for the next Portuguese cultural class starting on Jan. 6.
The Dance and Cheer Stars' annual Christmas show "Jingle Bell Rock" was conducted at the Neil Hafley School gym last weekend.
South San Joaquin Irrigation District wants to be able to help farmers and urban users outside of its district boundaries if the opportunity arrives in 2010.
Destiny Harper wants a Pixie game for Christmas.
Phil Waterford knows that times are tough for families during this holiday season.
Editor's note: The following is part of a series recapping Manteca history as the first decade of the 21st century draws to a close.
The Manteca Bulletin will not be publishing a Christmas edition on Friday so employees and carriers can spend the holiday with their families.
Scenes from the Boys & Girls Club Christmas party.
Scenes from Wednesday's performance of "The Reason for the Season" that took place at Manteca's Fellowship Baptist Church.
It took less than a week for a jury to convict Michael Roessler in the murder of 51-year-old rural Manteca Michael Lawrence.
The Manteca Kindred Arts has been bringing top-notch musicians of national and international caliber to Manteca for nearly three decades. Perhaps less known is the fact the non-profit organization has been offering other entertainment options every year for the public's gastronomical pleasure, among other things.


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I lost my front sight!
Sarge was out hunting with a buddy of his, Ed, hunting doves. Now Ed is a darn good shot. And when the two of them go hunting it is neck and neck as to who takes the most shots. The birds were coming in fast and furious and the shooting was great. The birds were ahead of the score but there were so many of the them that the ground was littered with empty shells. Now for non-hunters that is called FUN. Even though you miss 4 times for every one time you hit it is still the best hunting around.
By lunch time the barrels were hot and the hunters were hungry. So they found a nice log laying under a shady tree and pulled out the sandwiches. Ed was ahead of Sarge by 3 birds and it looked like he was on a roll. If he kept on the way he was going he was going to limit out before Sarge by mid afternoon. Mummm good sandwiches thought Sarge.
Suddenly Ed shouts. "I LOST MY FRONT SIGHT!"
"Huh, What, Where, You lost your front sight???" asks Sarge "That is impossible you are shooting a shotgun. They do not have sights."
"Look!" Ed points, " My front sight is gone!"
"You mean that little bead thingy?" asks Sarge
"Yeah, my front sight, it is gone!" wailed Ed.
"Silly, that is not a front sight." stated Sarge. You are not suppose to be using that thing like a rifle.
"What am I going to do, I can't shoot a shotgun without a front sight." cried Ed.
"Ed calm down, Ed buddy, you are not suppose to be using that little bead as a sight. You just look at the bird and swing the gun, when the gun touches the bird or passes it you pull the trigger. Shotguns do not have sights on them. You know why they put that little bead on the front of the shotgun barrel?" asks Sarge.
"No, don't they put it on there for the shooter to use?" asks Ed.
"No No, Ed, you are not thinking right. They just put that little bead on the front of the barrel to make a hunter feel good. Really, if you had a shotgun and it didn't have a little bead on it and everyone else had beads on their shotguns, you would feel bad. Then you would call up the gun company and ask them why they didn't put a little bead on your shotgun. They would sighhhh and say OK send it in to the factory and they will put a bead on it. When you got the gun back and there was a little bead on the barrel you would feel good. Seee" says Sarge
"Well how am I going to hit anything without a front sight?" asks Ed.
"Ed,Ed,Ed just look at the bird and cover it with the end of the barrel and pull the trigger." states Sarge.
But poor Ed, the bead had probably fallen off sometime very early in the morning, or it came off in the gun case when he pulled it out, but for the rest of the day Ed couldn't hit anything. Because he didn't have any front sight. When he didn't know it was gone he just shot instinctively. And he hit on most every shot. That afternoon he got a lot of shooting in, but Sarge filled his bag limit first. Ed got only one more bird all afternoon and that one was coming straight at him and he shot in self defence. (Instinctively)
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Did he ever find out that you used your Leatherman on his bead?
What we have here is... failure- to communicate.
Mine has a laser on the end.
I just blind the bird and let it fly into a tree.
Are you refering to the Miss Me Bead? I know every time I tried to use that thing I missed.
Patron Member NRA
"I would not be an old man if I had not been an armed young man." JTJ
I was taught to respect my elders but they are getting harder to find.
Can't you just use the 1911 sight embedded in your fore head?
I suppose I could have but the doctor took it out so he could bandage me up. I did consider trading guns with my buddy but then he might have beat me in number of birds. LOL


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Surname used as a given name. Variation of Stirling. Influenced during the 1940s by actor Sterling Hayden, who played notable roles in the 1964 film "Dr. Strangelove or: How I Learned to Stop Worrying and Love the Bomb" and "The Godfather" in 1972. Stirling is occasionally used in Great Britain.
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Civil Rights Leader Wade Henderson Speaks Out for Marriage Equality in Maryland
January 12, 2012 by HRC staff
Post submitted by Charlie Joughin, HRC Press Secretary
Today our friend Wade Henderson, president of the Leadership Conference on Civil and Human Rights, wrote an op-ed for the Baltimore Sun urging African-Americans to stand behind the fight for marriage equality in Maryland.
In the piece, titled "Blacks should stand with gays on marriage equality," Mr. Henderson mentions the injustice of denying gay and lesbian families the security that comes with marriage, saying "Marriage equality is not only a matter of civil rights; it's one of human rights and basic dignity. To deny familial and spousal benefits to millions of gay and lesbian Americans — benefits that are taken as a given for straight Americans — is to undermine the basic family unit that we in the black community have worked so hard to preserve and advance."
We are so grateful to Wade Henderson for speaking up in this fundamental fight for fairness. As you may know, HRC has teamed up with a number of organizations to form Marylanders for Marriage Equality, a strong coalition working for marriage for committed and loving gay and lesbian couples. For more information on the campaign, visit www.marylandersformarriageequality.org.
Click here to read the op-ed on the Baltimore Sun website.
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February 18, 2015


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Ask the Experts
Touched penis after removing condom.Hi,I had sex with a prostitue on 20th july. I used condom and i am sure it was not broken or leaked. But after removing condom, which may have some vaginal fluid, immediately i touched my penis head and tip and kept hand there for about 2 mins. Now...
Response from Robert J. Frascino, M.D.
The Robert James Frascino AIDS FoundationHello,Your question is similar to many others already posted in the archives. Have a look. Your HIV-acquisition risk is so remote it's essentially nonexistent. Your fears are unwarranted. If you wish to get a definitive HIV-antibody test, you'll... Read more »
HIV tests and boils on penisHi Doc,Condom failure during a sexual encounter with Russian CSW in Thailand around 28 days back. I... Read more »
My finger touched womens vegina and fluidRespected Sir,I had 2 months before finger (unprotected) sex with one call girl( . Within few... Read more »
Condoms...a toilet. They can clog plumbing. Using a Male Condom: Put the condom on when your penis is erect -- but before it touches your partner's mouth, vagina, or rectum. Many couples use a condom too late, after some initial penetration. ... Read more »
Viral Load Tests...test, which can be "negative" for 2 to 6 months after HIV infection. (See Fact Sheet 102 for more information on HIV antibody testing.) For prognosis , viral load can help predict how long someone will stay healthy. The higher the viral load,... Read more »
Understanding HIV-Related Lab Tests II: Viral Load, Resistance and Tropism...Viral Load Test Viral load is the amount of HIV in your bloodstream. Viral loadcan be measured by two different lab tests: polymerase chain reaction (PCR) orbranched DNA (bDNA). It is best to stick with the same kind of test and notswitch... Read more »
Safer Sex Guidelines...tested, or they might have gotten the test too soon after they were exposed to HIV. Fact Sheet 102 has more information on HIV testing. What if Both People Are Already Infected? Some people who are HIV-infected don't see the need to follow... Read more »
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Please Stop the Nightmare! HELP!! (very long)
Posted by losangelesman, 2 Replies...and take my 2 previous test results from Urologist and go find an Infectious Disease Specialist at the best hospital in LA. Tested again for all STDs and PCR, DNA/RNA, T-Cell count, etc. Again all tests NEG and the T-Cells are normal (1000-ish) ... Read more »
Please Advice
Posted by Me_Me, 2 Replies...aware if either had a tear but collectively it seemed intact i.e. no water leakage. I did not perform oral sex neither did I touch her vagina with my penis. I also did not kiss her. In the bathroom, after removing the condom, I put water over... Read more »
Please Help me
Posted by Anonymous, 1 Reply...person. The only worry i have is Reused condom . There was no penetrative sex but I mastrubated wearing a condom & my penis touched her thighs for 2-3 minutes. I dont know the quality of condom and whether the condom was a reused condom. I... Read more »
need an answer
Posted by Anonymous, 4 Replies...these expensive tests and my doc says they were not needed medically - only mentally. still, i wonder...is pcr rna and pcr dna testing at the early stage as accurate as an antibody test at 3/6 months. can someone please tell me? i want to know... Read more »
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Rectangle Teak Wood Ridge Tree BoxItem # HN-DTH002
- Solid teak construction
- Naturally resistant to moisture, warping and rot
- Does not require a liner
- Stainless steel hardware
- Teak footings and drainage holes
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HBR's 10 Must Reads Boxed Set125.00You want the most important ideas on management all in one place. Now you can have them--in a set of HBR's 10 Must Reads, available as a 6 volume paperback...
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Column: The End of the Middle ManagerTechnology Magazine articleThe technology revolution has brought us a lot—dramatic improvement in what we know about customers and how we interact with them, markedly better information for making decisions, the ability to work through virtual teams scattered around the globe. But its unseen legacy might be something much more fundamental: It has changed the very nature of […]
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Students Will Change the B-SchoolsEthics ArticleWhat do business school students and graduates do? What is their modus operandi? What do they bring to the party? Who are they, really? The business world is currently going through a profound re-evaluation. As a result of the current economic crisis, fundamental assumptions about the purpose of business, and the roles of those who […]


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Embroidered Hoodies
Starting at $50.00
Help customers warm up to your business.
- Put your own brand on this comfy, breathable essential
- Design your hoodies in minutes or upload your own logo
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Gildan's dry blend adult full zip hooded sweatshirt. 9.3 oz., 50/50 cotton/polyester fleece. Air jet spun yarn creates a softer feel without pilling. Double needle stitching and athletic rib with Lycra. Pouch pockets and set-in sleeves. Size and Material
Gildan's dry blend adult full zip hooded sweatshirt. 9.3 oz., 50/50 cotton/polyester fleece. Air jet spun yarn creates a softer feel without pilling. Double needle stitching and athletic rib with Lycra. Pouch pockets and set-in sleeves. Size and Material
Share a review and see what other customers are saying.
Embroidered Hoodies is rated out of 5 by 91.
Rated 5 out of 5 by Mudd Butt Baits from A lot nicer then ones from major store Heavy duty zipper, thick material and nice stitching.
Date published: 2015-03-18
Rated 1 out of 5 by cwat1040 from Poor Quality We really wanted to love these! Initially (out of the box) they looked good/acceptable unfortunately, after one wash it was rendered completely useless. The quality just isn't there
Date published: 2015-03-02
Rated 4 out of 5 by Odd Job Bob from Great Hoodie This product washes so well, it's 2 years old, and still looks good...
Date published: 2015-03-01
Rated 5 out of 5 by alpc from Awesome Product I love this product, the embroidery from Vistprint is awesome!
Date published: 2015-03-01
Rated 3 out of 5 by bitoluck from Embroidered Hoodie this is a very high quality sweatshirt, but the embroidered logo tends to shrink during washing. The circular logo has become oblong. Other than that, it's great.
Date published: 2015-02-23
Rated 2 out of 5 by M Staheli from The stitched part wasn't finished! When I got this the logo looked like it got snagged halfway done and then they never finished it. The sweater is a nice sweater. I just wish the design wouldn't have gotten messed up. Im kind of frustrated 6 of my 8 shirts and this sweater were bad. I placed a huge order all of my paper marketing materials were awesome like they always are! This is the first time i've bought clothing from vistaprint. It was bad. I should have listened to the reviews. The quality is good just didn't get the logos right :/
Date published: 2015-02-14
Rated 2 out of 5 by Beverly Hills from Letter are too close to each other I have been working with Vistaprint about 2years now, but i am not satisfied with my order this time. Letters are close to each other and it doesn't look professional, and by that i mean letter look funny.!!! Again, it is not the first time ordering from Vistaprint but it is definitely the last time.
Date published: 2015-02-10
Rated 5 out of 5 by Tonyp from A great quality Product I had vista print make me one of these when they first started doing the hoodies about 2-3 years ago. It is still in great shape and I wear it all of the time. I just ordered another one in a different color and it feels nice and heavy. great product and quality work.
Date published: 2015-02-07


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Phil Lynch was having a bad day at the track. Through the first five races of the 2004 Breeders' Cup championships at Lone Star Park, in Grand Prairie, Texas, Lynch hadn't picked a winner, hadn't cashed a ticket. But suddenly he flashed to a famous Seinfeld episode in which the George Costanza character is lamenting his failed life
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Welsbach, Baron Carl Auer von (1858-1929), an Austrian chemist. During the 1880's in the laboratory of Robert Wilhelm Bunsen, inventor of the Bunsen burner, he did research on rare-earth elements and their compounds. Von Welsbach noted that some of the compounds burned brilliantly, and he began to use them in gaslight appliances he invented, including the gas mantle. He discovered neodymium and praseodymium (1885), two of the rare-earth elements. In 1907, von Welsbach and G. Urbain (working independently) discovered lutetium and isolated ytterbium, two more rare-earth elements.
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Love this comforter. I purchased it myself and absolutely loved it!!! For anyone looking for a sophisticated look, definitely consider this. The taupe is a little darker than the picture but I can tell the picture was taken in a bright room because when I open my curtains and let some light in, my comforter brightens up. Just wish they had the whole set still(window treatment, european shams, and camelot rug are all NA)
High quality for the price. Beautiful set!
It was ok. If your wanting something really beige this could be it. The picture is not true to the color u get. But it was just ok.
like it very much
I have the complete bedroom set. The curtains look great as does the bedspread. I am very pleased with it.
I sent it back, costing me $34. The bed skirt did not match the comforter! Still waiting for c/c to be credited the return.
I love the style of this set. It has a feminine look to it, but also appeals to the masculine. There are two things you may want to know about this set. 1. The embroidery on the bedskirt is beautiful, but I have black threads on the floor here and there from where the stitching loosened up in the back. You can pull them back up by stretching the fabric, but this becomes a real pain when vacuuming. 2. As much as I love this comforter we can't use it! It's EXTREMELY warm - more like hot - and we live in a climate where the weather can get cold. When I bought this I also bought the black lace curtain to go with the set. Now, it doesn't look like Touch of Class has it anymore which is a shame because I'm moving and will have two windows in my room instead of only one.
Beautiful bedding, fast shipping, wonderful costumer service..I will definately come back ! Thank you Touch of Class
Great price and quality. I would buy again.
This bedroom ensemble looks great on line and I loved it; however, don't be fooled by the color you see on line. On line it looks like a platinum or a very light taupe. It is NOT a light taupe, it is a dark taupe color. I was extremely disapointed when I received this. The taupe makes this look very dark. I am returning as I cannot live with the darkness of the taupe.
i love the design and color on this, but the king size comforter was too small, did not cover the entire mattress so had to buy blankets in same color to not have a "gap" in color ;(


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Draft Update
The first round of the NFL Draft gets underway tonight and it won't take long for the Minnesota Vikings to take center stage. The Vikes currently own the No. 3 overall selection in the draft. …26 Apr 12
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The first round of the NFL Draft gets underway tonight and it won't take long for the Minnesota Vikings to take center stage. The Vikes currently own the No. 3 overall selection in the draft. …26 Apr 12
The three-day NFL Draft begins tonight with the first round at Radio City Music Hall. The Vikings currently hold the third-overall pick, but general manager Rick Spielman says the team is listening to trade offers. …26 Apr 12
The Minnesota Vikings continue to analyze the NFL Draft and figure out what they want to do with the third-overall pick in tomorrow night's first round. General Manager Rick Spielman says there's a chance they …25 Apr 12
FOX Sports' Jay Glazer reports the Vikings have agreed to a one-year deal with wide receiver Jerome Simpson. The news cames on the same day ESPN reported the former Bengals wideout will receive a three-game …25 Apr 12
The bill for a new Vikings stadium was approved by the Senate Rules Committee yesterday. According to the "Minneapolis Star Tribune," the bill could be up for a vote by the full Senate by Thursday …24 Apr 12
A bill to put a Vikings stadium in Minneapolis cleared a Senate committee last night (Fri) where it had been stalled for weeks. It's a partial recovery for stadium backers, whose bill went down to …21 Apr 12
The "Star Tribune" reports NFL commissioner Roger Goodell will be at the state capitol today to lobby for a new stadium for the Vikings. According to the report, Goodell will meet with Republican and DFL …20 Apr 12
(Eden Prairie, MN) – Here is the 2012 schedule for the Minnesota Vikings: Friday, August 10th – at San Francisco, 8pm (PRESEASON) Friday, August 17th – vs. Buffalo, 7pm (PRESEASON) Friday, August 24th – vs. …19 Apr 12
It's Winthrop Day today (THU) on KNUJ as our 2014 Town Days Tour continues. Mike Lemmer is broadcasting from Eagle's Landing in Winthrop this afternoon as we celebrate the community. Mike's guests will include Winthrop …


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Latest Headlines
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Novo Nordisk speeds back to the FDA with its once-rejected insulin
After a surprise FDA rejection sent it back to the drawing board in 2013, Novo Nordisk is finally ready to resubmit Tresiba, a long-acting insulin with blockbuster potential.
Victoza powers Novo's strong year; CEO projects growth for 2015
Novo Nordisk CEO Lars Sorensen told investors today that 2014 had been a more challenging year than most, then rolled off a series of results that showed significant leaps in sales both in the U.S. and worldwide. He then waved off any worries about a price war in the U.S. being a significant issue for the world's largest maker of insulin products this year.
Novo Nordisk walks back some U.S. optimism for its next-gen insulin
Novo Nordisk is sounding a more cautious tone on Tresiba, its once-rejected new insulin. After hinting at an accelerated trip back to the FDA for the long-acting diabetes treatment, the Danish drugmaker now says it could be another few years before the injection is ready for another shot at approval.
Novo finds new way to sell Tresiba in EU while awaiting another shot in U.S.
Novo Nordisk is hustling to get work done so that it can resubmit its blockbuster hopeful Tresiba to the FDA next year with an eye on getting into the market by 2016, three years after the FDA squelched its plans with its initial denial. In the meantime, Novo is finding new ways to combo Tresiba up and sell it in Europe where it is approved.
Novo's next-gen insulin clears a pediatric hurdle amid renewed U.S. hope
Novo Nordisk bolstered its better-late-than-never case to win FDA approval for a new long-acting insulin, as the drug, Tresiba, met its main goals in a study on children with Type 1 diabetes.
Novo Nordisk accepts a $90K slap on the hand for delaying news of Tresiba setback
Danish financial regulators took a dim view of Novo Nordisk's decision to spend the weekend pondering the FDA's February 2013 snub to Tresiba before spreading the news of the agency's complete response letter and the major delay that would be triggered by its demand for a new study. And today Novo said it had decided to accept a $90,000 fine to close the books on the incident, not the least bit chastened at the slap on the hand.
Novo chief Sorensen preps for showdown as Lilly's dulaglutide nears diabetes market
The executive team here at the rapidly expanding headquarters of Novo Nordisk doesn't pull many punches. CEO Lars Rebien S ø rensen is a competitive sort who tends to be blunt, aggressive and forward-thinking; projecting a confident attitude that is reflected by the rest of the execs and right down the line to 40,000 rank-and-file employees around the world.
Novo shoots for 2016 Tresiba launch as Q2 results come in strong
Diabetes market giant Novo Nordisk surprised investors today with second-quarter earnings that came in above expectations, and the news that it plans to report critical clinical trial data on its long-acting insulin treatment, Tresiba, in mid-2015, setting the drug up for a 2016 approval by the FDA.
Novo picks up the pace with its next-gen insulin, eyeing a 2016 U.S. debut
Last year's surprising FDA rejection blew a hole in Novo Nordisk's plans to capitalize on a new long-acting insulin, but the Danish drugmaker said its follow-on study is coming through ahead of schedule, clearing the way for a 2016 launch.
With new hopes for German pricing, Novo Nordisk decides to roll out Tresiba there
Novo Nordisk has decided to play ball in Germany. The Danish drugmaker now plans to launch its brand-new diabetes drug Tresiba in that country, despite a notorious pricing policy that Novo feared would interfere with its success.


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I give the shooting experience 2 stars for the following:
Firtst there was a problem with the booking whereby the event had to be reschedule.
So I had to do the shooting on the day we left Moscow, what was a bit inconvenient..
However the people of Moscow free tour did all there best to solve this problem.
Second I was very dissapointed that it was not possible to thake photo s at the shooting range, although this is mentioned in the description of the activity.
This makes it a bit expensive, because a good photo is surely what you need to brag with it at home!
Maybe its would be an idee to have a replica ak47 just for photo..
Pluspoint for the girl of Moscow free tour who guide us to the range, and the guy who assist the shooting itself. They where very friendly, enjoyable and helpful.
Doing what: Moscow Shooting Experience: Shoot an AK-47 at the Central Shooting Club
Especially our guide was awesome. She interested with us about any issue. Thank you for all.
Doing what: Moscow Shooting Experience: Shoot an AK-47 at the Central Shooting Club
Stuart F
United Kingdom
excellent guide, excellent knowledge. This is a must do.
Doing what: Moscow Metro Tour
I absolutely recommend this tour. Our guide was really cool. She picked us up on time and managed everything perfectly. It´s a little bit expensive, but it is worth it.
Doing what: Moscow Shooting Experience: Shoot an AK-47 at the Central Shooting Club
This trip was well worth the money. Great to see the beauty of the stations.
Doing what: Moscow Metro Tour
It brought an insight to me to understand the kind of communism in Russia. During the tour we didn't merely talk about communism, but also other terms regarding to Russia. A great thanks again to Elena, our tour guide, we had a great time.
Doing what: Communist Moscow: Evening Walking Tour
Anyone into architecture, definitely should take this tour. We were guided to various subway stations and none of them were alike. Each had it's own uniqueness, and own culture. Thanks a lot to Elena, our tour guide. She guided us in such a way which stirred my excitement about Russia even more.
However, the information on my voucher was misleading. It stated that the meeting point was:
"Biblioteka Lenina metro. KutafiaTower of the Moscow Kremlin"
As a matter of fact, Biblioteka Lenina Metro (the entrance where the sign is) and Kutafia Tower were two different places. The two were separated by a broad street, and not exactly across each other.
Doing what: Moscow Metro Tour
Tour guide, a young woman...arrived late, she did not speak English very well, nor could she understand English very well, we tried to communicate...but it did not go very far, so that was frustrating.....she didn't quite know what to do with us...took us to some places where it was the dark,some back streets... but it wasn't very interesting...there was extra time at the end, disappointment!
Doing what: Moscow at Night: Small-Group Walking Tour with Annushka Tram
Our Guide made a Great JOB we had a wonderful experience I strongly recommend to enjoy this tour in Moscow. My only problema was to find the meeting point. Really our tour guide put passion to the explanation to each detail an she speaks many languages including spanish!! A summary in one Word "GREAT"
Doing what: Moscow Metro Tour
Great overall experience. The guide took me across the city and gave me a good perspective on the history and things to see and do.
Doing what: Moscow Walking Tour: Architecture, History and Culture from a Local's Perspective
This walking tour was very informative. Our guide, Irina, was very knowledgable and had delightful positive energy. I was pleasantly surprised that we walked and visited so many places. Make sure you have comfortable walking shoes on, as there is alot of walking.
Doing what: Communist Moscow: Evening Walking Tour
Great tour with a very informative guide who spoke very good english.
Doing what: Early-Admission Kremlin Tour in Moscow: Armory, Diamond Fund Exhibition and Assumption Cathedral
Great tour guide Olaf (not sure of the spelling). Very resourceful and passionate person. He offered lots of ver useful information about different historical topics about Russia. Pleasant and very decent too. The tour itself was lovely covering the most important parts of Moscow. Thanks
Doing what: Kremlin Small-Group Tour
My walking tour was immensely interesting and the guide was knowledgeable and thorough in her commentary. I was leaving Moscow that day and did not have time to pursue independent tours of some of the sites seen on the walking tour but the guides recommendations and tips for best times to visit sites would have been invaluable should I have been able to stay longer in the city.
Doing what: Small-Group Moscow City Walking Tour
My wife and I arrived in Moscow and by the time we checked into our hotel we had to rush to the Biblioteka imeni Lenin Metro Station meeting point. There were other words but we thought being a Metro Tour the logical meeting point would have been at the entrance to the metro station. The instructions given on the booking printout gave the metro station as the primary followed by a tower of some sort. We were told that we were mere metres away from the tower when we went back to our hotel and was told by the concierge. For visitors to the big city and having to familiarize with the directions and names I think proper instructions in Bold should have been provided. The tour would have probably been a good tour but we can't give any ratings as we missed it.
Doing what: Moscow Metro Tour
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FDA approves Boston Scientific atrial fibrillation deviceMarch 16, 2015
The approval follows three advisory panel reviews and a complete response letter.
Device maker pays feds $2.8M over physician false claimsFebruary 12, 2015
Medtronic, as part of the settlement, will pay $602,000 to a whistleblower to settle charges it caused physicians to submit false claims to federal health programs.
J&J pulls morcellator from marketJuly 31, 2014
The company is calling the move a withdrawal, as opposed to a recall.
GE buys interest in DNAJanuary 06, 2014
The finance, electric and broadcasting company has taken a $1-billion interest in DNA technology.
Covidien buys firm armed with missile techDecember 09, 2013
Covidien is buying ingestible-camera company Given Imaging.
Report: J&J nearing hip settlementNovember 13, 2013
The current number is reported to hover around $4 billion.
FDA exempts most mobile apps from scrutinySeptember 24, 2013
The agency issued final guidance on mobile apps, saying it will focus only on a subset that present the greatest risk to patients if they don't work correctly.
"Sensor you swallow" passes psych testAugust 07, 2013
Proteus Digital Health's ingestible sensor shows utility as an adherence tool for those on psych meds, a study suggests.
Mayo device helps monitor heart patients from afarMay 22, 2013
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J&J consolidates, rebrands Ethicon unitsMay 08, 2013
Driven by consolidation in the healthcare industry, J&J is reuniting Ethicon, Inc. and Ethicon Endo-Surgery to present surgeons and health systems with a single face.
Abbott closes Q1 with sales boostApril 17, 2013
The company expects emerging markets to rise in importance and says it's scoping out possible device acquisitions.
Senate vote puts ACA's device tax in doubtMarch 22, 2013
In a big win for the device industry, Senators voted overwhelmingly on a symbolic— but politically potent—motion to scrap the 2.3% device excise tax.
WebMD digs deeper into the everydayMarch 21, 2013
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Growth in devices, online video to drive media this year, buyers sayJanuary 04, 2013
What changes in marketing or audience viewing habits might impact pharma's media investment this year? Media buyers weigh in on the hot channel trends.
DePuy coaches joint pain sufferers not to delay surgeryDecember 05, 2012
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Baxter to buy hemodialysis giant Gambro AB for $4 billionDecember 04, 2012
After almost a year of negotiations, Baxter has reached a $4 billion deal for Swedish firm Gambro AB as it makes a play for the kidney dialysis market.
Company news: MedMira; FDA device IDJuly 05, 2012
Diagnostics firm MedMira gets a contract from the US Army to develop a screening tool, and the FDA proposes some new device guidelines.
Endo rebrands to move beyond roots in pain drugsMarch 02, 2012
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Sanofi contest gooses diabetes tech innovationFebruary 23, 2012
Sanofi is re-upping its social media-driven Data Design Diabetes Innovation Challenge contest, which last year kicked up a wealth of innovative ideas and buzz for the price of $200,000 in prize money and a few salaries.
FDA to IOM: More work? No thanksAugust 11, 2011
The FDA is moving cautiously on a call by the Institute of Medicine to scrap the approvals process for many medical devices.
FDA guidance exempts most pharma apps from scrutinyJuly 19, 2011
FDA issued draft guidance on mobile medical apps, focusing narrowly on those programs that augment regulated medical devices or turn mobile devices into medical devices.
Mobile pill cap takes social approach to raising complianceJuly 07, 2010
A company that markets a pill cap with a built-in cellular connection puts a social twist on getting patients to take their meds.
Insulin pen makers roll out new promosMay 05, 2010
Sanofi-Aventis US is rolling out a consumer campaign for its Lantus SoloStar pen aimed at getting people with type 2 diabetes to think differently about insulin, while Novo Nordisk revs up another racing-themed social media effort for its FlexPen.
Bayer glucose meter aimed at gamersApril 27, 2010
Bayer Diabetes Care partnered with Nintendo on a glucose meter for kids that incorporates gaming.
Did you miss January's Top 40 Healthcare Transformers issue? Read how these inventors, strategists, entrepreneurs and wonks are challenging, disrupting and otherwise transforming the healthcare business. And join us April 30 to honor them at the Transforming Healthcare Dinner. Click here.
MMM Articles
- US pharma market value projected to be $550 billion in five years
- Lawmakers propose allowing pharma companies to share some off-label information
- Biosimilar uptake will require companies to tell a trustworthy backstory
- Everyday Health buys rare-disease agency Cambridge BioMarketing
- Apple's ResearchKit: Five Guidelines for Pharma


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House and Senate leaders said July 31 they have reached an agreement on funding the government into the first quarter of fiscal 2013.
Congressional leaders have agreed to cap spending under the continuing resolution at $1.047 trillion. It's the amount agreed to in the Budget Control Act in August 2011.
A continuing resolution keeps the government funding at current levels, if a formal appropriations bill has not been signed into law.
The fiscal year begins Oct. 1.
Reid said the agreement will provide stability for agencies in the coming months.
House Majority Leader John Boehner (R-Ohio) said members will draft legislation that the both chambers can pass and that President Barack Obama will sign into law.
The White House applauded the agreement.
"The agreement reached by House and Senate leadership to fund the government through the first quarter of 2013 is a welcome development," the White House said in a statement. "We are encouraged that both sides have agreed to resolve this issue without delay."
About the Author
Matthew Weigelt is a freelance journalist who writes about acquisition and procurement.
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Unisys Federal Systems' Venkatapathi Puvvada, this year's Eagle award winner for industry, stresses the importance of community.
Experts tell committee that business models, not technology, are the main obstacle to better sharing across systems.
Protests have delayed SEWP V, but industry experts say the contract vehicle offers agencies service and value that are hard to find.
The power of the individual informs FCW's coverage each and every day, but with the Federal 100, we take time to really spotlight and celebrate it. Get to know each of this year's winners.
The latest version of the Cybersecurity Information Sharing Act purportedly protects companies from liability and individuals from government intrusion.
Popular CIO is headed for the private sector.
The Census Bureau hasn't established a time frame for its cloud computing plans, including testing for scalability, security, and privacy protection, as well as determining a budget for cloud services.
President Obama's proposal to boost government coordination with the private sector got a warm welcome in the House Homeland Security Committee.
The spy agency wants to better integrate cybersecurity into its traditional human intelligence operations.
To RBB in Salt Lake: Is Salt Lake City ready to absorb 16,033 middle and high end jobs lost as a direct result of Sequestration??? Or a state GSP hit of S1,612,000,000.00? Take that much money out of your economy and see how hard it is even to get a job at a fast food business or retail store. Your housing market would tank also due to all the defaults! This would be the result of sequestration FY2013 budgets according to the Fuller Report: "The Economic Impact of the Budget Control Act of 2011 on DOD & non-DOD Agencies" Just a thought. Be careful what you ask for.
What is the point of having an opposition party if they do not stand up when the have a chance. We are running insane deficits year after year and neither party has the guts to say enough is enough. Boehner needs to be removed if the Republicans are to have any hope of ever being a party of principle. Otherwise, we simply have Democrats and Democrats light.
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The art itself is pretty bad in that page, but comparing with to the image of Rokudo above the elder brother's eyes are almost certainly the Sharingan. There's only two 'rings' around the pupil and if you look closely you can spot three telltale dots for the 'tomoes'.
One thing that's been puzzling me for over a year is what exactly happened to this great powerful Senju clan that's supposedly been ruling Konoha for generations. Tsunade's the only Senju we know of. None of the village elders(who apparently served the will of Senju) are Senju and neither is Danzo. Where the heck are they now?
Answer: Kishi just pulled the Senju-Uchiha clan rivalry out of his ass without properly considering what he had(or hadn't) revealed about the village hierarchy. But it'd be nice to at least have a shot of some Senju elders to explain this huge plot hole.


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27'''' Coronado
Anyone know where I can get the plans, specs for my boat. Need to know lines, electrical, etc.
Also I need a dingy 2 place fiberglas
27'''' Coronado
go to www.concentric.net/~Cs-cb/Sailing
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This Dinner Table Proves That People Will Drag Race Anything
Let's be honest, when it takes ten minutes just to get a lousy milkshake in the drive-thru, McDonald's isn't fast food. This is. Behold! The racing dinner table. »
Let's be honest, when it takes ten minutes just to get a lousy milkshake in the drive-thru, McDonald's isn't fast food. This is. Behold! The racing dinner table. » 12/30/14 6:30pm 12/30/14 6:30pm
Time for your weekly edition of the Deadspin Funbag. Got something on your mind? Email the Funbag. Today, we're covering partners, air beds, office superpowers, poop, and more. » 8/26/14 3:38pm 8/26/14 3:38pm
What if I told you that mozzarella sticks never had to end? That for $10, you could eat for free (for $10) for the rest of your natural life? That there exists a spot in the space-time continuum in which it is always Friday? That there are free refills on all Slushes™ excluding Red Bull® branded items?… » 7/18/14 9:20am 7/18/14 9:20am
If the world needed any more proof that Americans are some fattie mcfatties, we present the French-Fry Holder. This $10 device fits in cup holders and holds a standard cardboard container of french fries. It even has a small holder for ketchup, for those fattersons that need to add a little more flavor (and sodium) to… » 4/30/08 3:40pm 4/30/08 3:40pm
Many people may not realize that there's a significant geographic bias against the northern border states by Drive-In-Style fast food chains, but this map clearly shows one exists. The map shows states with the most Sonic Drive-Ins (red), followed by those with fewer than 125 stores (purple) and those with no stores… » 11/16/07 3:30pm 11/16/07 3:30pm
Getting treatment you'd only expect in Sadr City or Walter Reed, Marine Sergeant Michael Hutchinson ran into a fast food restaurant to grab some forgotten fries and returned to find an angry man, Mr. Glenn Merrill of Bedford, trying to move the Marine's Explorer (oh yeah, and the Marine's daughter was in the car).… » 3/22/07 12:20pm 3/22/07 12:20pm